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    <name>A0A8C4KV31_EQUAS</name>
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        <submittedName>
            <fullName evidence="6">PWWP domain containing 3B</fullName>
        </submittedName>
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    <gene>
        <name evidence="6" type="primary">PWWP3B</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the PWWP3A family.</text>
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        <property type="gene ID" value="ENSEASG00005000744.2"/>
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        <property type="protein sequence ID" value="ENSEASP00005059672.1"/>
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        <property type="term" value="C:extracellular exosome"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="PWP3A_3B_4"/>
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        <property type="entry name" value="PWP3A_3B_4_N"/>
    </dbReference>
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        <property type="entry name" value="PWWP DOMAIN-CONTAINING DNA REPAIR FACTOR 3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <begin position="5"/>
            <end position="99"/>
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            <begin position="388"/>
            <end position="467"/>
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        <location>
            <begin position="540"/>
            <end position="680"/>
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            <begin position="101"/>
            <end position="137"/>
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            <begin position="270"/>
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    <accession>A0A8C4KVR6</accession>
    <name>A0A8C4KVR6_EQUAS</name>
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            <fullName evidence="4">Cofilin 2</fullName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
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                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Guo"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Gao"/>
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                <person name="Li"/>
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                <person name="Li"/>
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                <person name="Hou"/>
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                <person name="Liu"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
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                <person name="Yue"/>
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                <person name="J."/>
                <person name="Liu"/>
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                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
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                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
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                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
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                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <text evidence="6">Could be involved in the unfolded protein response (UPR) pathway.</text>
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            <topology evidence="1">Single-pass membrane protein</topology>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="HERPUD1/2"/>
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    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
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    <dbReference type="PANTHER" id="PTHR12943:SF5">
        <property type="entry name" value="HOMOCYSTEINE-RESPONSIVE ENDOPLASMIC RETICULUM-RESIDENT UBIQUITIN-LIKE DOMAIN MEMBER 2 PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="HOMOCYSTEINE-RESPONSIVE ENDOPLASMIC RETICULUM-RESIDENT UNIQUITIN-LIKE DOMAIN HERPUD PROTEIN FAMILY MEMBER"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0834">Unfolded protein response</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="293"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="10">
        <location>
            <begin position="10"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="86"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="106"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="145"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023230"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005142.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="405" mass="44897" checksum="C4A1FD16556DF6CA" modified="2023-09-13" version="2">MDQNGMEIPVTLIIKAPNQKYSDQTISCFLNWTVGKLKTHLSNVYPSKPLTKDQRLVYSGRLLPDHLQLKDILRKQDEYHMVHLVCTSRTPPSSPKSSTSKESHEALASSSNSSSDQSESTTPSSSQEPLSLAASSSSEGLRQRTLPQAQTDPAQSPQFPYVMQGNVDNQLPGQAIPAGFPVYPGFSPLQMLWWQQMYAHQYYMQYQAAVSAQATSNASPAEPAASQPLNLAHVPGEEPPPAPNLVAQENRPMNENVQMNAQGGPVLNEEDFNRDWLDWMYTFSRAAILLSIVYFYSSFSRFIMVMGAMLLVYLHQAGWFPFRQEGQQQAPNNNAEVNNDGQNANNLELEEMERLMDDGVEDESGEDAGEDASALQRPGLMASAWSFITTFFTSLIPEGPPQVAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L894</accession>
    <name>A0A8C4L894_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">ATP-dependent RNA helicase TDRD9</fullName>
            <ecNumber evidence="4">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Tudor domain-containing protein 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">TDRD9</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with piRNA-associated proteins PIWIL1 and PIWIL4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="4"/>
    <dbReference type="Ensembl" id="ENSEAST00005006241.2">
        <property type="protein sequence ID" value="ENSEASP00005005712.2"/>
        <property type="gene ID" value="ENSEASG00005004203.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157035"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071547">
        <property type="term" value="C:piP-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009566">
        <property type="term" value="P:fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007141">
        <property type="term" value="P:male meiosis I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034587">
        <property type="term" value="P:piRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141196">
        <property type="term" value="P:transposable element silencing by piRNA-mediated DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141006">
        <property type="term" value="P:transposable element silencing by piRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20431">
        <property type="entry name" value="Tudor_TDRD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000073">
        <property type="entry name" value="ATP-dependent RNA helicase TDRD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001113">
        <property type="entry name" value="ATP-dependent RNA helicase TDRD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000012">
        <property type="entry name" value="putative ATP-dependent RNA helicase TDRD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000946">
        <property type="entry name" value="putative ATP-dependent RNA helicase TDRD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.90:FF:000016">
        <property type="entry name" value="Tudor domain containing 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047384">
        <property type="entry name" value="Tudor_TDRD9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF113">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE TDRD9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="12" id="KW-0744">Spermatogenesis</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="22">
        <location>
            <begin position="132"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="23">
        <location>
            <begin position="367"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="21">
        <location>
            <begin position="934"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="36"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00062441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00081664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005712.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1371" mass="154906" checksum="E558DDB388CA74A9" modified="2023-09-13" version="2">MLRKLTVDQINDWFTIGKTVANVELLGAPPAFPAEAARDEAQRRAAGPGSAQAQAQRAVSGRPLSQKSSEMEYINKYRQLEVQQFDMYGSEQSTSGPGPRPPLAKLSNVTCIPETTYKYPDLPINRCKEEVISLIESNSVVIIHGATGSGKSTQLPQYILDHYIQRSAYCNIVVTQPRKIGASSIARWISRERAWALGGLVGYQVGLEKIATGDTKLIYMTAGVLLQKLVSAKSLMEFTHVFIDEVHERTEEMDFLLLVVRKLLRTNSRFVKVVLMSATINCKDFADYFAVPVQNKMNPAYVFEVEGKPHSIEEYYLNDLEHVHHSRLSPHLLEEPVITKDIYEVAVSLIQMFDDLDMKESGKKTWSGAQFVSERSSVLVFLPGLGEINYMHELLTNMVHKRLQVYPLHSSVTLEEQNNVFLSPVPGYRKIILSTNIAESSVTVPDVKYVIDFCLTRTLVCDEDTNYQSLRLSWASKTSCNQRKGRAGRVSKGYCYRLIHKDFWDNSIPDHVVPEMLRCPLGSTILKVKLLDMGEPRALLATALSPPSLSDIERTILLLKEVGALAVSGQREDENPHDGELTFLGRVLAQLPVNQQLGKLIVLGHVFGCLDECLIIAAALSLKNFFAMPFRQHLDGYRNKVNFSGNSKSDCIALIEAFKTWQTCRQRGELRHPKDELDWGWLNYIQIKRIREVAELYEELKNRISQFNMHVDSRRPVMDQEYIYKQRFILQVVLAGAFYPNYFTFGPPDEEMAVRELAGKDPKTTVVLKHIPPYGFLYYKQLQSLFRQCGQVKSIVFDGSKAFVEFARNPTERFKTLPAVYMAIKMSQLKVSLELSVHSAEEIEGKVQGGTVSKLRNTRVNVDFQKQTVDALQVSLDTSDRPRTTTDLLLTIDVTEVVEVGHFWGYRIDEKNSEILRKLTAEINRLKLVPLPIHPHPDLVCLAPFSEFDRESYFRAQILYVSGNSAEVFFVDYGNRAHVELALLMEIPCQLLELPFQALEFKICKMRPSAKSLVCGEHWSGAASRRFASLVSGCTLLVKVFSVVHNVLHVDVYRYSGVQDTISIRDVLIKEGYAELAEESFESKQSHEVLKSLFSKSVENVTDISVPSPVKDDEKYLIRILLESFSSNKLGTPNCKAILHGPFNPYELKCHSLTRISKFRCVWIEKESINSVIISDTPEDLHQRMLVAASLSVNATGSTMLLRETSLMPHIPGLPALLSMLFAPVMELRVDRDGKCYTGVLCGLGWNPSTGAPVLPEHDIELAFDVQFSVEDIVEINILRAAINKLVCDGPNGSKYLGPERIAQLQDNARQKLLGLFCQLKPRERIVPNWHEKPYEWNQVDPKLVMEQADQESSRGNTFLYQLHKLIVLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L8Q1</accession>
    <name>A0A8C4L8Q1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acetyl-CoA acyltransferase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ACAA2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L8Q1"/>
    <dbReference type="Ensembl" id="ENSEAST00005008055.2">
        <property type="protein sequence ID" value="ENSEASP00005007382.2"/>
        <property type="gene ID" value="ENSEASG00005005395.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234626"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000010">
        <property type="entry name" value="Acetyl-CoA acetyltransferase (Thiolase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919:SF107">
        <property type="entry name" value="ACETYL-COA ACETYLTRANSFERASE, CYTOSOLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18919">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="8">
        <location>
            <begin position="4"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="9">
        <location>
            <begin position="271"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="6">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="349"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007382.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="394" mass="41568" checksum="241DCE0F3ED4F45C" modified="2023-09-13" version="2">RMSVFIVAAKRTPFGAYGGLLKDFTAIDLTEFAAKAALSAGKVSPEIVDSVIVGNVLQSSSDAIYVARHVGLRVGIPIATPALTVNRLCGSGFQSIVNGCQEICVKDAEVVLCGGTENMSQAPFCVRNVRFGTRLGSDLKLEDTLWTSLTDTHVQLPMAITAENLAVKHKISREDCDKYALQSQQRWKAANDAGYFNNEMAPIEVKTKKGKQIMQVDEHARPQTTLEQLSKLPPVFKKEGTVTAGNASGVSDGAGAVIIASEDAVKKHNFTPLARIVGYFVSGCDPSLMGIGPVPAINGALKKTGLSLKDMDLVEVNEAFAPQYLAVEKSLNLDPSKANVNGGAIALGHPLGGSGSRITAHLVHELRRRGGKYAVGSACIGGGQGIAVIIENTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L962</accession>
    <name>A0A8C4L962_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tropomyosin alpha-1 chain</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the tropomyosin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L962"/>
    <dbReference type="Ensembl" id="ENSEAST00005008018.2">
        <property type="protein sequence ID" value="ENSEASP00005007350.2"/>
        <property type="gene ID" value="ENSEASG00005031100.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234542"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000005">
        <property type="entry name" value="Tropomyosin alpha-1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000533">
        <property type="entry name" value="Tropomyosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19269">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00261">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00194">
        <property type="entry name" value="TROPOMYOSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
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        <location>
            <begin position="81"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00009036"/>
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        <source>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Google" id="ProtNLM"/>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Huang"/>
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                <person name="Zhao"/>
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                <person name="Wang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Yang"/>
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                <person name="Ju"/>
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                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="G."/>
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                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
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                <person name="S."/>
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                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022796"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008741.2"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="78" mass="8784" checksum="B9B4B64FD61AB9A3" modified="2023-09-13" version="2">MKKRETPGKHQFSVDLTCEGWYNAVTQGLTKLGEVQFDIDLPSKKVRIDCEYSVDSLLETLRQTGKSVLESSLFFLGA</sequence>
</entry>
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    <accession>A0A8C4LCV2</accession>
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                <person name="Yu"/>
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                <person name="Jin"/>
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                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
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                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
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                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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        <property type="entry name" value="COX7a"/>
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        <property type="entry name" value="Cyt_c_oxidase_su7a_sf"/>
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    <dbReference type="InterPro" id="IPR003177">
        <property type="entry name" value="Cytc_oxidase_su7a_met"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10510">
        <property type="entry name" value="CYTOCHROME C OXIDASE POLYPEPTIDE 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10510:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 7A-RELATED PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02238">
        <property type="entry name" value="COX7a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037710">
        <property type="entry name" value="COX7A-rel_mt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81419">
        <property type="entry name" value="Mitochondrial cytochrome c oxidase subunit VIIa"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009331"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="116" mass="12924" checksum="00D68C77E112BBBF" modified="2023-09-13" version="1">MYYKFSGFTQKLAGAWASEAYSPQVRGLKPLVSTEAPPIIFATPTKLTSDSTAYDYAGRNKVPELQRLFQKSDGVPIHLKRGLPDQMLYRTTMALTLGGTIYCLIALYMASQPRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LD22</accession>
    <name>A0A8C4LD22_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Keratin 35</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">KRT35</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LD22"/>
    <dbReference type="Ensembl" id="ENSEAST00005010097.2">
        <property type="protein sequence ID" value="ENSEASP00005009283.2"/>
        <property type="gene ID" value="ENSEASG00005006641.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161787"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000002">
        <property type="entry name" value="Type I keratin 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF193">
        <property type="entry name" value="KERATIN, TYPE I CUTICULAR HA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="6">
        <location>
            <begin position="97"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="101"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="207"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="292"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009283.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="455" mass="50379" checksum="4F790877C851544F" modified="2023-09-13" version="2">MASKCLKASFSSGSLKVPGGAGGGSARMSTMYSSSSCRLPSLSRGARSFSACSVGLGKSSCRAASCLPALCLPSGGFATSLSIGGGWFGEGILTGNEKETMQSLNDRLASYLEKVRQLEQENASLESRIREWCEQQVPFLCPDYQSYFRTIEEIQKKTLCTKAENAKLVVQIDNAKLAADDFRTKYEMEVSMRQLVESDMNGLRRILDDLTLCKADLEAQVESLKEELLCLKKNHEEEVNSLRCQLGDRLNVEVDAAPPVDLNRVLDEMRCQYETLVENNRRDAEDWFDTQTEELNRQVVSSSEQLQSCQAEIIELRRTVNALEIELQAQHSTRDALESTLAETEARYSSQLAQVQGLISNVEEQLSEIRADLERQNQEYQVLLDVKARLESEINTYRGLLESEDCKLPCNPCTPDHSPSKSCLPCLPAASCGPGAARPTCRPRPICVPCPGGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LDQ8</accession>
    <name>A0A8C4LDQ8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">2-amino-3-carboxymuconate-6-semialdehyde decarboxylase</fullName>
            <ecNumber evidence="4 12">4.1.1.45</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Picolinate carboxylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ACMSD</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Converts alpha-amino-beta-carboxymuconate-epsilon-semialdehyde (ACMS) to alpha-aminomuconate semialdehyde (AMS). ACMS can be converted non-enzymatically to quinolate (QA), a key precursor of NAD, and a potent endogenous excitotoxin of neuronal cells which is implicated in the pathogenesis of various neurodegenerative disorders. In the presence of ACMSD, ACMS is converted to AMS, a benign catabolite. ACMSD ultimately controls the metabolic fate of tryptophan catabolism along the kynurenine pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2-amino-3-carboxymuconate 6-semialdehyde + H(+) = 2-aminomuconate 6-semialdehyde + CO2</text>
            <dbReference type="Rhea" id="RHEA:16557"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:77634"/>
            <dbReference type="ChEBI" id="CHEBI:77803"/>
            <dbReference type="EC" id="4.1.1.45"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Secondary metabolite metabolism; quinolate metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily. ACMSD family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.45" evidence="4 12"/>
    <dbReference type="RefSeq" id="XP_014704981.1">
        <property type="nucleotide sequence ID" value="XM_014849495.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LDQ8"/>
    <dbReference type="Ensembl" id="ENSEAST00005011412.2">
        <property type="protein sequence ID" value="ENSEASP00005010488.2"/>
        <property type="gene ID" value="ENSEASG00005007438.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106836535"/>
    <dbReference type="KEGG" id="eai:106836535"/>
    <dbReference type="CTD" id="130013"/>
    <dbReference type="GeneTree" id="ENSGT00490000043417"/>
    <dbReference type="OrthoDB" id="191270at2759"/>
    <dbReference type="UniPathway" id="UPA00270"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001760">
        <property type="term" value="F:aminocarboxymuconate-semialdehyde decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006569">
        <property type="term" value="P:L-tryptophan catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904985">
        <property type="term" value="P:negative regulation of quinolinate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019748">
        <property type="term" value="P:secondary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000029">
        <property type="entry name" value="2-amino-3-carboxymuconate-6-semialdehyde decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032465">
        <property type="entry name" value="ACMSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240">
        <property type="entry name" value="2-AMINO-3-CARBOXYLMUCONATE-6-SEMIALDEHYDE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21240:SF27">
        <property type="entry name" value="2-AMINO-3-CARBOXYMUCONATE-6-SEMIALDEHYDE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04909">
        <property type="entry name" value="Amidohydro_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="9 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="13">
        <location>
            <begin position="3"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005871"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012365"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021214"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005010488.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="336" mass="37736" checksum="85554442255A8015" modified="2023-09-13" version="2">MKIDIHSHILPKEWPDLKKKFGYGGWVQLQHHSKGEAKMVKDGQVFRVVQENCWDPEVRIREMDKTGVTAQALSTVPVMFSYWAKPQDTLDLCQLLNNDLAATVASHPRRFVGLGTLPMQAPELAIKEMERCVNELGFPGVQIGSHVNEWDLNAQELFPVYAAAEKLNCSLFVHPWDMQMDGRMAKYWFPWLVGMPAETTTAICSMIMGGVFEKFPKLKVCYAHGGGSFPFTVGRISHGFSMRPDLCAQDNPTNPKKYLGSFYTDSLVHDPLALKLLTDVIGKDKVILGTDYPFPLGELEPGKLIESIEEFDAETKDKLKAGNALAFLGLERKLFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LFC1</accession>
    <name>A0A8C4LFC1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">rRNA adenine N(6)-methyltransferase</fullName>
            <ecNumber evidence="14">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">TFB2M</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>adenosine in rRNA + S-adenosyl-L-methionine = N(6)-methyladenosine in rRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:58728"/>
            <dbReference type="Rhea" id="RHEA-COMP:15198"/>
            <dbReference type="Rhea" id="RHEA-COMP:15199"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74449"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT. In this complex TFAM recruits POLRMT to the promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Interacts with mitochondrial RNA polymerase POLRMT. Interacts with TFAM.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 14">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="14"/>
    <dbReference type="RefSeq" id="XP_014695589.1">
        <property type="nucleotide sequence ID" value="XM_014840103.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LFC1"/>
    <dbReference type="Ensembl" id="ENSEAST00005008576.2">
        <property type="protein sequence ID" value="ENSEASP00005007857.1"/>
        <property type="gene ID" value="ENSEASG00005005729.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106830524"/>
    <dbReference type="KEGG" id="eai:106830524"/>
    <dbReference type="CTD" id="64216"/>
    <dbReference type="GeneTree" id="ENSGT00950000183142"/>
    <dbReference type="OMA" id="IFEVPWT"/>
    <dbReference type="OrthoDB" id="9895503at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034246">
        <property type="term" value="F:mitochondrial transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006391">
        <property type="term" value="P:transcription initiation at mitochondrial promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000209">
        <property type="entry name" value="rRNA adenine N(6)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF13">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027833">
        <property type="entry name" value="MtTFB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13 14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 14" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 13 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 13 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034057220" description="rRNA adenine N(6)-methyltransferase" evidence="15">
        <location>
            <begin position="16"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA adenine methylase transferase N-terminal" evidence="16">
        <location>
            <begin position="95"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00062295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="398" mass="45899" checksum="4D90B28262C1B81D" modified="2022-01-19" version="1">MWVPVAGLPPRLTLSALTGACRLCILGSGAARRKDVLARDRRGLSDFHPQLLPSLSFGDSPSWVTKCRLEPRRYITSPRLAETLVQVLRGGREKTCQLFLECNPGPGILTQALLENGARVIALESDRTFIPHLESLGKNLNGKLEVVHCDFFKLDPTNCGTVKPPIMVSKMLFQNLGIQALPWSKGIPLKVIGIFPIKNERKTLWKLLHDLYSCTSIYRYGRVELNMFISEKQYQKLMANPKNPNLYQALSVLWQVACEIKLLHMEPWSSFDIYTQDGQLEKPKRRESLEEVQQNLCLIQLTPRRTLFTENLTPINYDVFFHMLKQCFMKRNAKLIDHLHSLSPVDAMDILKKIKKKEKTKITNLYPRDFKQLFETIECSKDYTCKWLYDDFLEDRIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LGV7</accession>
    <name>A0A8C4LGV7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">phosphatidylinositol-3,4-bisphosphate 4-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.66</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">INPP4B</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
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                <person name="M."/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1D-myo-inositol 3,4-bisphosphate + H2O = 1D-myo-inositol 3-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:43388"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:83241"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Signal transduction; phosphatidylinositol signaling pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inositol 3,4-bisphosphate 4-phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.66" evidence="3"/>
    <dbReference type="RefSeq" id="XP_070360920.1">
        <property type="nucleotide sequence ID" value="XM_070504819.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070360921.1">
        <property type="nucleotide sequence ID" value="XM_070504820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LGV7"/>
    <dbReference type="Ensembl" id="ENSEAST00005012066.2">
        <property type="protein sequence ID" value="ENSEASP00005011096.2"/>
        <property type="gene ID" value="ENSEASG00005007803.2"/>
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    <dbReference type="GeneTree" id="ENSGT00940000157587"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:phosphatidylinositol-3,4-bisphosphate 4-phosphatase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Type II inositol 3,4-bisphosphate 4-phosphatase"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="C2_domain_sf"/>
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        <property type="entry name" value="INPP4"/>
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    <proteinExistence type="inferred from homology"/>
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            <begin position="20"/>
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        <source>
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        <source>
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        <source>
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        </source>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011096.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="924" mass="104907" checksum="310B4956C1C4D53B" modified="2023-09-13" version="2">MEIKEEGASEEGQHFLPTPANDTGDFQFTSIQKTPNEPQLEFILACKDLVAPVRDRKLNTLVQISVIHPAEQSLTRYSSTEIVEGTRDPLFLTGVTFPSDYPIYEETKIKLTVYDVKDKSQDTVRTSVLLEHKDPLPEVGRSFLGYASFKVGELLKSKEQLLALSLRTSDGGKVVGTIEVSLVKMGEIEDGEADHITTDVQGQKCALVCECTAPESVSGKDNLPFLNAVLKNPVCKLYRFPTSDNKWMRIREQMSESILSFHIPKELISLHIKEDLCRNQELKELGELSPHWDNLRKNVLTHCDQMVNMYQGILKELSKETGSSFKSSSSKGERTLEFVPVNLHLQRMHVHSPHLKDALYDVITVGAPAAHFQGFKNGGLRKLLHRFETERRNTEYQFIYYSPENTAKAKEVLSNINQLQPLIATYADLLLNSASQHSPDSLKNSLKMLSEKTELFVHAFKDQLVRSALLALYTARPGGILKKPPSPKNSTEESSPQDPPPPMKRQDSIPHHSDYDEEEWDRVWANVGKSLNCIIAMVDKLIERDGGSGGSSSSSKNEEKDPSLVDSIITHPREDWYEQLYPLILTLKDCMGEVVNRAKQSLTFVLLQELAYSLPQCLMLTLRRDIVFSQALAGLVCGFIIKLHTSLHDPGFLQQLHTVGLVVQYEGLLSTYSDEIGMLEDMAVGISDLKKVTFKITEAKSNDVLPVITGRREHYVVEVKLPARMFESLPLQIKEGQLLHVYPVLFNVGINEQQTLAERFGDVSLQESINQENFELLKEYYTIFMEKMPPDYISHFQEQNDLKGLLENLHQNIQAKKRKNVEIMWLAATICRKLNGIRFTCCKSAKDRTSMSVTLEQCSILRDEHQLHKDFFIRALDCMRREGCRIENVLKNIKCRKYAFNMLQLMAFPKYYRPPEGTYGKADT</sequence>
</entry>
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        <submittedName>
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        <name evidence="2" type="primary">LAS1L</name>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="G."/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
                <person name="J."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="RIBOSOMAL BIOGENESIS PROTEIN LAS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15002:SF0">
        <property type="entry name" value="RIBOSOMAL BIOGENESIS PROTEIN LAS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04031">
        <property type="entry name" value="Las1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="184"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="506"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="694"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="513"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="556"/>
        </location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="563"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009843.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="741" mass="85150" checksum="325766B927D9CCB8" modified="2023-09-13" version="2">MDRVWSAWCGKCVQERGTSPLWAQRIVVAWLSKAEWDQVTVYLFSDDHKLQRYALNRITVWRSRLGNELPLAVASTADLVRCKLMDVTGGLGTDELRLLYGMALVRFVNLISERKTKFVKLPLKFLAQEVNIPDWIVELRHELTHKKMPHINDCRRGCYFVLDWLQKTYWCRQLENSLRETWELEEDREETDEEDQEDDKNIVVEDVTEQRPEPKDEAKGAELDVRADGDSEGNKEVDSHWEKTLRHKELYERARELLVSYEEEQFKVLEKFRHLPQAIKAWNNLSPPVECILAELKGITWENREAVLDAFLDDGFLIPTFEQLAALQIDYEENVDLNDILVPKPFSQFWQPLLRGLHSQTFTQALLERMLSELPALGDSGIRPTYILRWTLELIVANTKTGRNARRFSASQWEARRSWRLFNCSASLDWPRVVESCLGSPCWASPQLLQLVFKAMGQVLPDGEQEKLLRICSIYNQSGENSLVQEGSEASPIGKSPYTLDNLYWSLKPDGASSGPEGEAQQQEEQGSLNDVKKAEEEEKEVLEDQVEEEEEENDDQEKWKEETEDEEEEEEDDDDDDDDDDDDIDDEEDRMEVGPFSMGEESPTAENARLLAQKRGALQGSAWQVSSEDVRWDIFPLGRMPGQTEDPAELMLENYDTMYLLDQPVLEQRLEPPTCKTGHLGSKACAGLHALPRNSPLDHRRGSPWVIREPGTKQPLLRGSAGNTKEPVQRACTFRNAGPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LHZ6</accession>
    <name>A0A8C4LHZ6_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">EH domain binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">EHBP1</name>
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    <organism evidence="9 10">
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            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
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                <person name="Zhang"/>
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                <person name="Yin"/>
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            <dbReference type="Ensembl" id="ENSEASP00005016136.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="577" mass="65615" checksum="8C71BD4CA3E30D02" modified="2023-09-13" version="2">MDICIDLQTNYAELVLDVGRVTLGEKNRNKMKDRQLRKKQNDKISRAVCAELNSGGGVIKAEIENENYSFKTDGIGLDLENSFGNLLPCVRKYLDFMQQDNYFLIFVKSWSSEIPGLKIVTLSSNLYKRDITSAKVMDPIAALEFLKDRKERGEVLSPKPLSPPKRLCFGVQEESNIEAAAADFFNRMELRYKEKLTFTESTHVEMKQFSTEKWFQRIKEILPQYVSAFANTDGGYLFIGVSEDKQVIGLRAESSDLDKWKGEIETSIRKLPVYHFCGRNREIKYSHKFLGVYDEGHLCGYVCALKIQRFCCAVFAKEPDSWHVEDNRVRQMTVNEWIRFMVDAEPSEEGPHFSSLFEEMGLQRSRLSPIPRSQPVDNCKHLESWEQRCHLPVPSEKTTYVPEALYMKLFLQHEGLAQVVHKAVGSVHQGTLIFSRSWSLDLGLQENENVICDALLISKDCPPVLYTFLRVLDEGLKSYSIQTALTLKQKLAKIGGYTGKVCILTKTLSLSEESSTSTEGSAGLHHGSSLPALYPTPYNCITSETMKDLKKALFVVLKRQRSSSEQFASEVSQHLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LV62</accession>
    <name>A0A8C4LV62_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Alanine aminotransferase 1</fullName>
            <ecNumber evidence="12">2.6.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Glutamate pyruvate transaminase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Glutamic--alanine transaminase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Glutamic--pyruvic transaminase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">GPT</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-alanine + 2-oxoglutarate = pyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:19453"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="EC" id="2.6.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.2" evidence="12"/>
    <dbReference type="RefSeq" id="XP_070338019.1">
        <property type="nucleotide sequence ID" value="XM_070481918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LV62"/>
    <dbReference type="SMR" id="A0A8C4LV62"/>
    <dbReference type="Ensembl" id="ENSEAST00005017123.2">
        <property type="protein sequence ID" value="ENSEASP00005015744.2"/>
        <property type="gene ID" value="ENSEASG00005010803.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106841923"/>
    <dbReference type="CTD" id="2875"/>
    <dbReference type="GeneTree" id="ENSGT00940000155265"/>
    <dbReference type="UniPathway" id="UPA00528">
        <property type="reaction ID" value="UER00586"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004021">
        <property type="term" value="F:L-alanine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042853">
        <property type="term" value="P:L-alanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1970:FF:000001">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000236">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000345">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045088">
        <property type="entry name" value="ALAT1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751">
        <property type="entry name" value="ALANINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751:SF308">
        <property type="entry name" value="ALANINE AMINOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="19">
        <location>
            <begin position="110"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00025708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00026106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00059280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00076222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005015744.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="496" mass="54983" checksum="58953254B44BAAF7" modified="2023-09-13" version="2">MALRASDHSQAAMNRLKEKVLTLDTMNPCVRRVEYAVRGPIVLRALELEQELRQGIKKPFTEVIRANIGDAQAMGQKPITFLRQVLALCIHPDLLNSPNFPEDAKRRAEHILQACGGHSLGAYSVSSGIQLIREDVARYIQQRDGGIPADPNNIFLSTGASDAIVTVLKLLVAGEGHTRTGVLIPIPQYPLYSATLAELNAVQVDYYLDEERAWALDVAELRRALCQARDHCHPRALCVINPGNPTGQVQPRECIEAVIRFAFEEQLFLMADEVYQDNVYAEGSQFHSFKKVLMEMGPPYAAQQELASFHSVSKGYMGECGFRAGYVEVVNMDAAVQQQMQKLMSVRLCPPVPGQALLHVVVSPPAPSDPSFAQFQAERQAVLAELAAKAKLTEQVFNEAPGIHCNPVQGAMYSFPRVQLPPRAVLRAQELGLAPDMFFCMCLLEETGICVVPGSGFGQREGTYHFRMTILPPVEKLRSLLEKLSQFHAKFTRQYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LVH2</accession>
    <name>A0A8C4LVH2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Proteasome activator complex subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Proteasome activator 28 subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PSME2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of PSME1 and PSME2, which forms a hexameric ring.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PA28 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LVH2"/>
    <dbReference type="Ensembl" id="ENSEAST00005018716.2">
        <property type="protein sequence ID" value="ENSEASP00005017230.2"/>
        <property type="gene ID" value="ENSEASG00005011933.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183098"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
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    </evidence>
    <sequence length="72" mass="7994" checksum="6710809504D1DD53" modified="2023-09-13" version="2">MAAVKTPSRELKNAEEPFNNVSPVLLKSLVEKPKGRTEVPNHLLESKIYAKLLNNKVIQAKPGILHFGGYQV</sequence>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Yang"/>
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                <person name="Li"/>
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                <person name="Liu"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="Ji"/>
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                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="Li"/>
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                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <location>
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                <person name="X. and Zhong"/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000057">
        <property type="entry name" value="Short stature homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
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    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
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    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
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    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052631">
        <property type="entry name" value="Paired_homeobox_Bicoid"/>
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    <dbReference type="PANTHER" id="PTHR46255">
        <property type="entry name" value="SHORT STATURE HOMEOBOX"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR46255:SF1">
        <property type="entry name" value="SHORT STATURE HOMEOBOX PROTEIN 2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
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    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
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    <keyword evidence="3 6 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 6 7" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="9">
        <location>
            <begin position="140"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="10">
        <location>
            <begin position="315"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="6">
        <location>
            <begin position="142"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="28"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="59"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
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            <begin position="95"/>
            <end position="111"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="120"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50803"/>
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            <dbReference type="Ensembl" id="ENSEASP00005017901.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="333" mass="35111" checksum="96768BCCB8091900" modified="2023-09-13" version="2">MEELTAFVSKSFDQKVKEKKEAITYREVLESGPLRGAKEPTGCAEPCRDDRSSPAVRAAGGGGGGGGGGGSGGGGGGGGAGGGGAGGGAGGGRSPVRELDMGAAERSREPGSPRLTEVSPELKDRKEDAKGMEDEGQTKIKQRRSRTNFTLEQLNELERLFDETHYPDAFMREELSQRLGLSEARVQVWFQNRRAKCRKQENQLHKGVLIGAASQFEACRVAPYVNVGALRMPFQQDSHCNVTPLSFQVQAQLQLDSAVAHAHHHLHPHLAAHAPYMMFPAPPFGLPLATLAADSASAASVVAAAAAAKTTSKNSSIADLRLKAKKHAAALGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M3X6</accession>
    <name>A0A8C4M3X6_EQUAS</name>
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        <submittedName>
            <fullName evidence="8">NALCN channel auxiliary factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">NALF2</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NALF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4M3X6"/>
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        <property type="gene ID" value="ENSEASG00005012674.2"/>
    </dbReference>
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        <property type="component" value="Chromosome X"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015275">
        <property type="term" value="F:stretch-activated, monoatomic cation-selective, calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055288">
        <property type="entry name" value="NALCN_aux_factor_1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15819:SF8">
        <property type="entry name" value="NALCN CHANNEL AUXILIARY FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15819">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN FAM155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="67"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="112"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029445"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005018372.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="460" mass="51115" checksum="16689020D4D59312" modified="2023-09-13" version="2">SPRAACAAICCCCCCWAPRPSDKPCADSERAQRWRLSLASLLFFTVLLADHLWLCAGARPRARELSSAMRPPWGAGRERQPVPPRSVLPLSPPPPSAPPGTCGPRYSNLTKAAPAAGPGPDCGGVPEPTGLDAACTKLQSLQRLFEPTTPAPPLRPPDSPSRAPAEFPSAKKNLLKGHFRNFTLSFCDTYTVWDLLLGMDRPDSLDCSLDTLLGDLLAVVASPGSGAWEVCSNCIEAYQRLDRHAQEKYDEFDLVLHKYLQAEEYSIRSCTKGCKAVYKAWLCSEYFSVTQQECQRWVPCKQYCLEVQTRCPFILPDNEEMVYGGLPGFICTGLLDTSPRRPETKCCDVQWVSCESEKKKFKESEAPKTHHQQFHHSYFHHYHQQYHHYHPRHDPPGRISHKPSLLPVSGGSRLSPSRIRLYVLVLMLLHTMVSFSSNQGGGGLGLEALPALDEGLTREE</sequence>
</entry>
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    <accession>A0A8C4M8J1</accession>
    <name>A0A8C4M8J1_EQUAS</name>
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            <fullName evidence="20">Long-chain-fatty-acid--CoA ligase ACSBG1</fullName>
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        <alternativeName>
            <fullName evidence="21">Acyl-CoA synthetase bubblegum family member 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">ACSBG1</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="S."/>
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                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="X."/>
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                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
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                <person name="G."/>
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                <person name="Pan"/>
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                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
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                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Catalyzes the conversion of fatty acids such as long-chain and very long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation. Can activate diverse saturated, monosaturated and polyunsaturated fatty acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(E)-hexadec-2-enoate + ATP + CoA = (2E)-hexadecenoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:36139"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
            <dbReference type="ChEBI" id="CHEBI:72745"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15421"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57560"/>
            <dbReference type="ChEBI" id="CHEBI:83139"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>hexadecanoate + ATP + CoA = hexadecanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:30751"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Microsome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the ATP-dependent AMP-binding enzyme family. Bubblegum subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.3" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4M8J1"/>
    <dbReference type="Ensembl" id="ENSEAST00005024868.2">
        <property type="protein sequence ID" value="ENSEASP00005022917.2"/>
        <property type="gene ID" value="ENSEASG00005015616.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160380"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031957">
        <property type="term" value="F:very long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051384">
        <property type="term" value="P:response to glucocorticoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000038">
        <property type="term" value="P:very long-chain fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd05933">
        <property type="entry name" value="ACSBG_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000021">
        <property type="entry name" value="Long-chain-fatty-acid--CoA ligase ACSBG1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000023">
        <property type="entry name" value="Long-chain-fatty-acid--CoA ligase ACSBG1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF93">
        <property type="entry name" value="ACYL-COA SYNTHETASE BUBBLEGUM FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23562">
        <property type="entry name" value="AMP-binding_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0492">Microsome</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="25">
        <location>
            <begin position="116"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00026121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00038034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00040478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00043191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00045256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00049177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005022917.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="728" mass="81197" checksum="09C486D085F85B41" modified="2023-09-13" version="2">MPEVLLLRSASSILRTVFLSRLPPGGPGCVHKLSLNLRDQQGLRRNVGSFLTDGQTLSKASPRHAPEFSSPEKATDARLDSPEEALWTTRADGRVHLRMDPSCPQPPYTVHRMFYEALDKYGDLSALGFKRQGTWEYISYSQYYLLARKAAKGFLKLGLERAHSVAILGFNSPEWFFSAVGTVFAGGIVTGIYTTSSPEACQYIAQDCHANVIIVDTQKQLEKILKIWKNLPHLKAVVTYREPPPKKMANVYTMEEFMEVGNEVPEEALDTIIDAQQPNQCCVLVYTSGTTGNPKGVMLSQDNITWTARYGSQAGDIQPAEVQQEVVVSYLPLSHIAAQIYDLWTGIQWGAQVCFAEPDALKGSLVNTLREVEPTSHMGVPRVWEKIMERIQEVAAQSGFIWRKMLLWAMSVNLEQNLTCPGSDLKPFTTRLADYLVLAKVRQALGFARCQKNFYGAAPMTVETQNFFLGLNIRLYAGYGLSETSGPHFMSSPYNYRLYSSGKVVPGCQVKLVNQDAEGIGEICLWGRTIFMGYLNMEDKTCEALDADGWLHTGDAGRLDADGFLYITGRLKELIITAGGENVPPVPIEEAVKTELPIISNAMLIGDQRKFLSMLLTLKCTLDPDTSDPTDNLTEWAVEFCQRVGSKATTVSEIVQKKDEAVYQAIEEGIQRVNMNAAAQPYHIQKWAILERDFSISGGELGPTMKLKRLIVLEKYKDIIDSFYQEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M8L7</accession>
    <name>A0A8C4M8L7_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Semaphorin 3G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SEMA3G</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014724266.1">
        <property type="nucleotide sequence ID" value="XM_014868780.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M8L7"/>
    <dbReference type="Ensembl" id="ENSEAST00005020554.2">
        <property type="protein sequence ID" value="ENSEASP00005018940.2"/>
        <property type="gene ID" value="ENSEASG00005013045.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106848695"/>
    <dbReference type="KEGG" id="eai:106848695"/>
    <dbReference type="CTD" id="56920"/>
    <dbReference type="GeneTree" id="ENSGT00940000157677"/>
    <dbReference type="OrthoDB" id="9988752at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030517">
        <property type="term" value="P:negative regulation of axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05871">
        <property type="entry name" value="Ig_Sema3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000015">
        <property type="entry name" value="Semaphorin 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000030">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1680.10:FF:000001">
        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
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            <begin position="1"/>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M9M1</accession>
    <name>A0A8C4M9M1_EQUAS</name>
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        <submittedName>
            <fullName evidence="8">Tigger transposable element derived 4</fullName>
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        <name evidence="8" type="primary">TIGD4</name>
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        <name type="scientific">Equus asinus</name>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="Huang"/>
                <person name="J."/>
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                <person name="Min"/>
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                <person name="J."/>
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                <person name="Liu"/>
                <person name="Q."/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="HTH_Tnp_Tc5"/>
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        <location>
            <begin position="12"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="HTH CENPB-type" evidence="7">
        <location>
            <begin position="75"/>
            <end position="146"/>
        </location>
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    <feature type="DNA-binding region" description="H-T-H motif" evidence="4">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="437"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MAT9</accession>
    <name>A0A8C4MAT9_EQUAS</name>
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        <submittedName>
            <fullName evidence="2">Negative elongation factor complex member B</fullName>
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        <name evidence="2" type="primary">NELFB</name>
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        <name type="synonym">Equus africanus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Huang"/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
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                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MAT9"/>
    <dbReference type="Ensembl" id="ENSEAST00005025858.2">
        <property type="protein sequence ID" value="ENSEASP00005023836.2"/>
        <property type="gene ID" value="ENSEASG00005016044.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012665"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032021">
        <property type="term" value="C:NELF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008283">
        <property type="term" value="P:cell population proliferation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000737">
        <property type="term" value="P:negative regulation of stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034244">
        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048863">
        <property type="term" value="P:stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010405">
        <property type="entry name" value="COBRA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13503:SF3">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13503">
        <property type="entry name" value="NEGATIVE ELONGATION FACTOR COMPLEX MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06209">
        <property type="entry name" value="COBRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="599"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005023836.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="627" mass="69697" checksum="8028B0DA5F2A509F" modified="2023-09-13" version="2">AELEGAGERSAGGPRGPGERAAGGPAAAPGERGGDGAPSRAAAGASAMFAGLQDLGVANGEDLKETLTNCTEPLKAIEQFQTENGVLLPSLQSALPFLDLHGTPRLEFHQSVFDELRDKLLERVSAIASEGKAEERYKKLEDLLEKSFSLVKMPSLQPVVMCVMKHLPKVPEKKLKLVMADKELYRACAVEVKRQIWQDNQALFGDEVSPLLKQYILEKESALFSTELSVLHNFFSPSPKTRRQGEVVQKLTQMVGKNVKLYDMVLQFLRTLFLRTRNVHYCTLRAELLMSLHDLDVGDICSVDPCHKFTWCLDACIRERFVDSKRARELQGFLDGVKKGQEQVLGDLSMILCDPFAINTLSLSTVRHLQELVGQETLPRDSPDLLLLLRLLALGQGAWDMIDSQVFKEPKMEVELITRFLPTLMSFVVDDHTFNVDQKLPAEEKAPVTYPNTLPESFTKFLQEQRMACEVGLYYVLHITKQRNKNALLRLLPGLVETFGDLAFGDIFLHLLTGNLALLADEFALEDFCSSLFDGFLLTASPRKENVQRHVLRLLVHLHHRVAPSKLQALQKALEPTGQSGEAVKELYSQLGEKLEQLDHQKPSPAQTAETPALELPLPTVPAPAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MBB5</accession>
    <name>A0A8C4MBB5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="29">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="34" type="primary">LYN</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="34 35" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="25 29">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
            <topology evidence="5">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="29">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="29"/>
    <dbReference type="RefSeq" id="XP_044600129.1">
        <property type="nucleotide sequence ID" value="XM_044744194.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MBB5"/>
    <dbReference type="Ensembl" id="ENSEAST00005026155.2">
        <property type="protein sequence ID" value="ENSEASP00005024115.1"/>
        <property type="gene ID" value="ENSEASG00005016369.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106841654"/>
    <dbReference type="KEGG" id="eai:106841654"/>
    <dbReference type="CTD" id="4067"/>
    <dbReference type="GeneTree" id="ENSGT00940000158011"/>
    <dbReference type="OMA" id="TGNMGCI"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046875">
        <property type="term" value="F:ephrin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141038">
        <property type="term" value="F:phosphatidylinositol 3-kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140031">
        <property type="term" value="F:phosphorylation-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097110">
        <property type="term" value="F:scaffold protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030546">
        <property type="term" value="F:signaling receptor activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001782">
        <property type="term" value="P:B cell homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042100">
        <property type="term" value="P:B cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050853">
        <property type="term" value="P:B cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038159">
        <property type="term" value="P:C-X-C chemokine receptor CXCR4 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071300">
        <property type="term" value="P:cellular response to retinoic acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097028">
        <property type="term" value="P:dendritic cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030222">
        <property type="term" value="P:eosinophil differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030218">
        <property type="term" value="P:erythrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015908">
        <property type="term" value="P:fatty acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002774">
        <property type="term" value="P:Fc receptor mediated inhibitory signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002431">
        <property type="term" value="P:Fc receptor mediated stimulatory signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002244">
        <property type="term" value="P:hematopoietic progenitor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038043">
        <property type="term" value="P:interleukin-5-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031663">
        <property type="term" value="P:lipopolysaccharide-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030889">
        <property type="term" value="P:negative regulation of B cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050859">
        <property type="term" value="P:negative regulation of B cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070373">
        <property type="term" value="P:negative regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070667">
        <property type="term" value="P:negative regulation of mast cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002762">
        <property type="term" value="P:negative regulation of myeloid leukocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034136">
        <property type="term" value="P:negative regulation of toll-like receptor 2 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034144">
        <property type="term" value="P:negative regulation of toll-like receptor 4 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150076">
        <property type="term" value="P:neuroinflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002576">
        <property type="term" value="P:platelet degranulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902993">
        <property type="term" value="P:positive regulation of amyloid precursor protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000670">
        <property type="term" value="P:positive regulation of dendritic cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
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            <taxon>Eutheria</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="2">Forms a complex composed of PDGFRL, TNK2 and GRB2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MDT9"/>
    <dbReference type="Ensembl" id="ENSEAST00005027295.2">
        <property type="protein sequence ID" value="ENSEASP00005025142.2"/>
        <property type="gene ID" value="ENSEASG00005017112.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106828208"/>
    <dbReference type="KEGG" id="eai:106828208"/>
    <dbReference type="CTD" id="5157"/>
    <dbReference type="GeneTree" id="ENSGT00390000017153"/>
    <dbReference type="OrthoDB" id="9864753at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005017">
        <property type="term" value="F:platelet-derived growth factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000223">
        <property type="entry name" value="Platelet-derived growth factor receptor beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000907">
        <property type="entry name" value="Platelet-derived growth factor receptor-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001395">
        <property type="entry name" value="Platelet-derived growth factor receptor-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042495">
        <property type="entry name" value="PDGFRL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15360">
        <property type="entry name" value="PLATELET-DERIVED GROWTH FACTOR RECEPTOR LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15360:SF1">
        <property type="entry name" value="PLATELET-DERIVED GROWTH FACTOR RECEPTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21339">
        <property type="entry name" value="VEGFR-1-like_Ig-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040325140" description="Platelet-derived growth factor receptor-like protein" evidence="11">
        <location>
            <begin position="22"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="272"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="20"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025142.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="375" mass="42081" checksum="15480B5663700015" modified="2023-09-13" version="2">MKVWLLLGLLLVHVALEDVTGQHPPKNKRPKEPGENRIKPTNKKVKPKIPKIKDRDSADSMPKSQSIMIQMLDKGRFQKPAATLSLMAGQTLELRCKGNRIGWSYPAYLDTFKDSRLSVKQQERYGQLTLVNSTAADTGEFSCWGQLCNGYICRRDEAKTGSTYIFFTEKGELFVPSPSYFDVVYLNPDRQAVVPCRVTVLSAKVTLHREFPAKEIPANGTDIVYDMKRGFVYLHPHSDHQGVVYCKAEAGGKSQISVKYQLLYVEVPSGPPSTTILASSNKVKGGDDISVLCTVLGEPDVEVEFRWIYPGQKDERPVRIQDTWRLIHRGLAHTTRISQSVMTVEDFETIDAGYYICTAQNLRGQTTVATTVEFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MEL4</accession>
    <name>A0A8C4MEL4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RAD50-interacting protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">RAD50 interactor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RINT1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RINT1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014687360.1">
        <property type="nucleotide sequence ID" value="XM_014831874.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MEL4"/>
    <dbReference type="Ensembl" id="ENSEAST00005027749.2">
        <property type="protein sequence ID" value="ENSEASP00005025556.1"/>
        <property type="gene ID" value="ENSEASG00005017414.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106825221"/>
    <dbReference type="CTD" id="60561"/>
    <dbReference type="GeneTree" id="ENSGT00390000017006"/>
    <dbReference type="OMA" id="GMTWEVL"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070939">
        <property type="term" value="C:Dsl1/NZR complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060628">
        <property type="term" value="P:regulation of ER to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.670:FF:000003">
        <property type="entry name" value="RAD50-interacting protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.670">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042044">
        <property type="entry name" value="EXOC6PINT-1/Sec15/Tip20_C_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007528">
        <property type="entry name" value="RINT1_Tip20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13520:SF0">
        <property type="entry name" value="RAD50-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13520">
        <property type="entry name" value="RAD50-INTERACTING PROTEIN 1 RINT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04437">
        <property type="entry name" value="RINT1_TIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51386">
        <property type="entry name" value="RINT1_TIP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00061158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00073943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00079551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="792" mass="90263" checksum="3276B217E3A116C4" modified="2022-01-19" version="1">MLQAGEIGEAPATPSCSESGEERKNVEQKSDLNIAALVGNEPVGEGTDSGGLSSYVSAFIEKEVGNDLKSLKKLDKLIEQMTESKMQLEEQVLTISSEIPKRIQSALKNAEESEQFLHQFLEQEIHLSSSINSHLLTAQPWMEDLGAMINQIEEIERHLAYLKWISQIEELSDNIQQYLMTNNVPEAASTLVSMAELDIKLQESSCTHLLAFMRATVKFWHKILKDKLTSDFEEILAQLHWPFIAPSQSQTVGLSRPAGSSEIYSNLETLFCQLLKLQTSDELLTEPKQLPEKYSLPASPSVILPIQIMLTPLQKRFRYHFRGNRQTNVISKPEWYLAQVLLWIGNHTQFLDEKIQPILDKVGSAVSARLEFSRGLVMLVLEKLAADIPCLLYDDNLFCHLVDEVLLFERELHSVHGYPGTFANCMHILSEETCFQRWLTVERKFALQKMDSMLSSEAAWISQYKDITDVDEMKVPDCAETFMMLLLVITDRYKNLPTASRKLQFLELQKDLVDDFRIRLTQVMKEETRAPLGFRYCAILNAVNYISTVLADWADNVFFLQLQQAALEVFAENSALSKLQLGQLAAMESSVFDDMVNLLERLKHDMLTRQVDHVFREVKDAAKLYKKERWLSLPSQSEQAVMSLSSSACPLLLTLRDRLLQLEQQLCFSLFKIFWQMLVEKLDVHIYQEIILANHFNEGGAAQLQFDMTRNLFPLFSHYCKRPENYFKHVKEACIVLNLNVGSALLLKDVLQSASGQPPATAALNEVGIYKLAQQDVEILLNLRTNWPNTGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MGU7</accession>
    <name>A0A8C4MGU7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TNIK</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_014722309.1">
        <property type="nucleotide sequence ID" value="XM_014866823.3"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005027447.2">
        <property type="protein sequence ID" value="ENSEASP00005025280.1"/>
        <property type="gene ID" value="ENSEASG00005017205.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106847525"/>
    <dbReference type="CTD" id="23043"/>
    <dbReference type="GeneTree" id="ENSGT00950000183196"/>
    <dbReference type="OMA" id="MDTGTEY"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06637">
        <property type="entry name" value="STKc_TNIK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000003">
        <property type="entry name" value="TRAF2 and NCK-interacting protein kinase isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000006">
        <property type="entry name" value="TRAF2 and NCK-interacting protein kinase isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001180">
        <property type="entry name" value="CNH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051700">
        <property type="entry name" value="STE20_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47096">
        <property type="entry name" value="MISSHAPEN LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47096:SF1">
        <property type="entry name" value="MISSHAPEN LIKE KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00780">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00036">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50219">
        <property type="entry name" value="CNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="25"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="CNH" evidence="13">
        <location>
            <begin position="1047"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="284"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="398"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="540"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="601"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="814"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="908"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="933"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="317"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="652"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="720"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="775"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="814"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="834"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="908"/>
            <end position="917"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="987"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1360" mass="154819" checksum="23D3DD73B239C8DC" modified="2022-01-19" version="1">MASDSPARSLDEIDLSALRDPAGIFELVELVGNGTYGQVYKGRHVKTGQLAAIKVMDVTGDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKNPPGMDDQLWLVMEFCGAGSVTDLIKNTKGNTLKEEWIAYICREILRGLSHLHQHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDFKSDLWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPAPRLKSKKWSKKFQSFIESCLVKSHSQRPATEQLMKHPFIRDQPNDRQVRIQLKDHIDRTKKKRGEKDETEYEYSGSEEEEEENDSGEPSSILNLPGESTLRRDFLRLQLANKERSEALRRQQLEQQQRENEEHKRQLLAERQKRIEEQKEQRRRLEEQQRREKELRKQQEREQRRHYEEQMRREEERRRAEHEQEYIRRQLEEEQRQLEILQQQLLHEQALLLEYKRKQLEEQRQAERLQRQLKQERDYLVSLQHQRQEQRPVEKKPLYHYKEGMSPSEKPAWAKEVEERSRLNRQSSPAMPHKVANRISDPTLPPRSESFSISGVQPARTPPMLRPVDPQIPHLVAVKSQGPALTASQSVHEQPTKGLSGFQEALTVTSHRVEMPRQNSDPTSENPPLPTRIEKFDRSSWLRQEEDIPPKVPQRTTSISPALARKNSPGNGGALGPRLGSQPIRASNPDLRRTEPVLESPLQRTSSGSSSSSSTPSSQPSSQGGSQPGSQAGSSERTRVRANSKSEGSPVLPHEPSKVKPEESRDITRPSRPASYKKAIDEDLTALAKELRELRIEETNRPLKKVTDYSSSSEESESSDEEEEDGESEPHDGTVAVSDIPRLIPTGAPGSAEQYNVGMVGSHGLEPSHADTFSSSISREGTLMIRETSGEKKRSGHTDSNGFAGHINLPDLVQQSHSPAGTPTEGLGRVSTHPQEMDSGTEYGMGSSTKASFTPFVDPRVYQTSPTDEDEEDEESSAAALFTSELLRQEQAKLNEARKISVVNVNPTNIRPHSDTPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYNLINRRRFQQMDVLEGLNVLVTISGKKNKLRVYYLSWLRNRILHNDPEVEKKQGWITVGDLEGCIHYKVVKYERIKFLVIALKNAVEIYAWAPKPYHKFMAFKSFADLQHKPLLVDLTVEEGQRLKVIFGSHTGFHVIDVDSGNSYDIYIPSHIQGNITPHAIVILPKTDGMEMLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIHSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVFFMTLNRNSMMNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MK25</accession>
    <name>A0A8C4MK25_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SLC6A18</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014703973.2">
        <property type="nucleotide sequence ID" value="XM_014848487.3"/>
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    <dbReference type="Ensembl" id="ENSEAST00005029522.2">
        <property type="protein sequence ID" value="ENSEASP00005027191.2"/>
        <property type="gene ID" value="ENSEASG00005018374.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835879"/>
    <dbReference type="KEGG" id="eai:106835879"/>
    <dbReference type="CTD" id="348932"/>
    <dbReference type="GeneTree" id="ENSGT00940000158906"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031526">
        <property type="term" value="C:brush border membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140931">
        <property type="term" value="F:neutral L-amino acid:sodium:chloride symporter activity"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:renal amino acid absorption"/>
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        <property type="entry name" value="SNS_sf"/>
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        <property type="entry name" value="Na_transp"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="INACTIVE SODIUM-DEPENDENT NEUTRAL AMINO ACID TRANSPORTER B(0)AT3"/>
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    <dbReference type="Pfam" id="PF00209">
        <property type="entry name" value="SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00176">
        <property type="entry name" value="NANEUSMPORT"/>
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        <property type="entry name" value="ORPHTRNSPORT"/>
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    <dbReference type="SUPFAM" id="SSF161070">
        <property type="entry name" value="SNF-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NA_NEUROTRAN_SYMP_1"/>
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    <dbReference type="PROSITE" id="PS50267">
        <property type="entry name" value="NA_NEUROTRAN_SYMP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 9" id="KW-0769">Symport</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="115"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="179"/>
            <end position="198"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="210"/>
            <end position="233"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="253"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="290"/>
            <end position="311"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="402"/>
            <end position="422"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="443"/>
            <end position="462"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="474"/>
            <end position="497"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="518"/>
            <end position="540"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="567"/>
            <end position="593"/>
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    <feature type="binding site" evidence="8">
        <location>
            <position position="33"/>
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        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <label>1</label>
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    <evidence type="ECO:0000256" key="9">
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            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="616" mass="68659" checksum="9AB8FF94DF834D36" modified="2023-09-13" version="2">MALAPGPDPLGDGSGDERPKWDNKLQYLLSCVGFAVGLGNIWRFPYLCQTYGGGAFLIPYFIALAFEGIPLFHMELAIGQRLRKGSIGVWTTISPYLGGIGLGCFTVSSLIGIYYNTVLTWVLWYLLNSFQNPLPWSACPPDRNRTGFVEECQRSSSVSYFWYRQTLNVTADINDSGSIQWQLLVCLVASWAVVYLCIIRGIETTGKAIYFTALFPYLVLTIFLIRGLTLPGATEGLTYLFTPKMQILQNPRVWLDAATQIFFSLSLAFGGHIAFSSYNPPRNNCKKDAVTIALVNSMTSLYAAIIVFSVLGFKAANDHGRCLDRNILHLINAFDFPDQSISRDDYAAVLAHLNVTQPERVAGLGLESCRLEDFLDKSASGTGLAFIVFTEAILHMPGAPGWAVLFFGMLFTLGLSSMFGNMEAVITPLLDMGVMPRWVPKEVLTGVACLSCFLASIHFTLHSGNYWLEVFDRYAASLNLVIFAFLEVVGIVYVYGMNRFCDDVAWMTGRRPSLYWRVTWKVVSPLLLLTIFVAYIALLARVPPSYRAWNPQYDQFPTQQENVYPGWVQVTCVLLSFLPVLLVPAVALAQLLVRHRRKKKDALQDMHLKPQDSGAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MM80</accession>
    <name>A0A8C4MM80_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Potassium channel subfamily K member 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">TWIK-related arachidonic acid-stimulated potassium channel protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">KCNK4</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Cs(+)(in) = Cs(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78555"/>
            <dbReference type="ChEBI" id="CHEBI:49547"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>Rb(+)(in) = Rb(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78547"/>
            <dbReference type="ChEBI" id="CHEBI:49847"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="21">Homodimer; disulfide-linked. Forms heterodimers with other 2-pore domain K(+) channel subunits, such as KCNK2 and KCNK10.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Axon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 24">Belongs to the two pore domain potassium channel (TC 1.A.1.8) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044618053.1">
        <property type="nucleotide sequence ID" value="XM_044762118.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MM80"/>
    <dbReference type="Ensembl" id="ENSEAST00005031892.2">
        <property type="protein sequence ID" value="ENSEASP00005029346.1"/>
        <property type="gene ID" value="ENSEASG00005019945.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106833677"/>
    <dbReference type="KEGG" id="eai:106833677"/>
    <dbReference type="CTD" id="50801"/>
    <dbReference type="GeneTree" id="ENSGT00940000160310"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034705">
        <property type="term" value="C:potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098782">
        <property type="term" value="F:mechanosensitive potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022841">
        <property type="term" value="F:potassium ion leak channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071468">
        <property type="term" value="P:cellular response to acidic pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904551">
        <property type="term" value="P:cellular response to arachidonate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071260">
        <property type="term" value="P:cellular response to mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990478">
        <property type="term" value="P:response to ultrasound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030322">
        <property type="term" value="P:stabilization of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000106">
        <property type="entry name" value="Potassium channel subfamily K member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003280">
        <property type="entry name" value="2pore_dom_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008074">
        <property type="entry name" value="2pore_dom_K_chnl_TRAAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003:SF30">
        <property type="entry name" value="POTASSIUM CHANNEL SUBFAMILY K MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11003">
        <property type="entry name" value="POTASSIUM CHANNEL, SUBFAMILY K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01333">
        <property type="entry name" value="2POREKCHANEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01691">
        <property type="entry name" value="TRAAKCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 24" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12 24" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0630">Potassium</keyword>
    <keyword evidence="9" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="6" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 24 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 24" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="116"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="144"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="195"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="27">
        <location>
            <begin position="112"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel" evidence="27">
        <location>
            <begin position="206"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="311"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="25">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="25">
        <location>
            <begin position="346"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="25">
        <location>
            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006666"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022538"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022826"/>
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            <dbReference type="ARBA" id="ARBA00022958"/>
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            <dbReference type="ARBA" id="ARBA00023065"/>
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            <dbReference type="ARBA" id="ARBA00023157"/>
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            <dbReference type="ARBA" id="ARBA00023180"/>
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            <dbReference type="ARBA" id="ARBA00023273"/>
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            <dbReference type="ARBA" id="ARBA00034430"/>
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            <dbReference type="ARBA" id="ARBA00044657"/>
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            <dbReference type="ARBA" id="ARBA00044691"/>
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            <dbReference type="ARBA" id="ARBA00063569"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00068468"/>
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        <source>
            <dbReference type="RuleBase" id="RU003857"/>
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    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF07885"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005029346.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="421" mass="44981" checksum="82845E9A65747F98" modified="2022-01-19" version="1">MTAAPQEPPARPPQAGSGTGRAPGRAMRSTTLLALLALVLLYLVSGALVFQALEQPHEQQAQRELGEVREKFLRAHPCVSDPDLGLFIKEVADALGGGANPDTNSTSNSNHSAWDLGSAFFFSGTIITTIGYGNAALRTDAGRIFCIFYALVGIPLFGILLAGVGDRLGSSLRRGIGHIEAIFLKWHVPPELVRVLSAVLFLLVGCLLFVLTPTFVFCYVEGWSKLEAIYFVVVTLTTVGFGDYVAGASPNQNSAAYQPLVWFWILLGLAYFASVLTTIGNWLRVVSRRTRAEMGGLTAQAASWTGTVTARVTQRAGPAAPPPPEKERPPLPPLPCPAQPAGRPLSPAPPEKAEPPSPPTASALDYPSENLAFIDESSDTQSERGCALPRAPRGRRRPPNPPRKPARPRGPGRARDKGVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MRY4</accession>
    <name>A0A8C4MRY4_EQUAS</name>
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        <recommendedName>
            <fullName evidence="11">Neurexin 3</fullName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
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                <person name="X."/>
                <person name="Zhao"/>
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                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Y."/>
                <person name="Yang"/>
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                <person name="F."/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="G."/>
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                <person name="W."/>
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                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endomembrane system</location>
            <topology evidence="5">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the neurexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MRY4"/>
    <dbReference type="Ensembl" id="ENSEAST00005032328.2">
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        <property type="gene ID" value="ENSEASG00005020218.2"/>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="neurexin 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050372">
        <property type="entry name" value="Neurexin-related_CASP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036">
        <property type="entry name" value="PIKACHURIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15036:SF85">
        <property type="entry name" value="SP2353, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Laminin G" evidence="7">
        <location>
            <begin position="27"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="198"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MV60</accession>
    <name>A0A8C4MV60_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Rho GTPase activating protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ARHGAP4</name>
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            <taxon>Mammalia</taxon>
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    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="Y."/>
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                <person name="Jiang"/>
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                <person name="Wang"/>
                <person name="X."/>
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                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Yang"/>
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                <person name="Liu"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="11" key="3">
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                <person name="Li"/>
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                <person name="Liu"/>
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                <person name="X. and Zhong"/>
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    <sequence length="566" mass="66075" checksum="79596D83D8EBA64B" modified="2023-09-13" version="2">MSSHFLPIALTSGDKANDDSVKKDGPYKIFFKDLFLFKENEIAAKKKEKFMNRSMKVHQKSTFSSRMKSRSHLSQTVFYSETTGGSFEKFGLDPTLILRLTEGSDTKRTINEFITDQRNRFLLEFALLTKRNTIKSLEEHIATKEKQLMKAENKLQEDAMAFEEFLRENDQRSVDALKMAAQETINKLQMTAELKKASMEIQAVKSEMAKTEFLLREYMKYGFFLLKLSPKHWQIQQKMKRVQVSKSKENMNVTLPKILTKQDTKKKEGNIEEPRRTSFSEDYSLGKASQGKPHKKSTLILENKKSSLSNQAESISSEDSLEFFLDDDIDYDLEPELYFKEPEELLQVFTELEEQNLTLVQYSQDVDENLEDVNKREKFIQDKINNNIEFLLKHKKMLQANCVREEEKAAELELRSRLFSFGQFNSDAQEKLIESLSKKINQVYKVCIGDGEVGSLNPVQKLVKVESRLVELSDLIESIPKENVEAIERIKQKERRQKLREEKIKEKQKHQEERLKAALERAVAQPKKKLGRRLVYRSKPPSGNKQELFLVKDTRTKSLEEEYFFT</sequence>
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    <accession>A0A8C4MYQ1</accession>
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            <fullName evidence="13">GS homeobox 1</fullName>
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        <name evidence="13" type="primary">GSX1</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
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    <comment type="similarity">
        <text evidence="2">Belongs to the Antp homeobox family.</text>
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        <property type="entry name" value="HD"/>
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    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
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            <fullName evidence="6">Tumor necrosis factor alpha-induced protein 6</fullName>
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            <taxon>Vertebrata</taxon>
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            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">Interacts (via Link domain) with inter-alpha-inhibitor (I-alpha-I) component bikunin. Interacts with ITIH2/HC2; this interaction is required for transesterification of the HC to hyaluronan. Interacts (via Link and CUB domains) with ITIH1. Chondroitin sulfate may be required for the stability of the complex. Interacts (via Link domain) with various C-X-C and C-C chemokines including PF4, CXCL8, CXCL11, CXCL12, CCL2, CCL7, CCL19, CCL21, and CCL27; this interaction interferes with chemokine binding to glycosaminoglycans. Interacts (primarily via Link domain) with BMP2; this interaction is inhibited by hyaluronan. Interacts (via both Link and CUB domains) with TNFSF11. Interacts (via CUB domain) with FN1 (via type III repeats 9-14); this interaction enhances fibronectin fibril assembly. TNFAIP6 may act as a bridging molecule between FN1 and THBS1.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014831267.3"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:carboxylesterase activity"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:fibronectin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005540">
        <property type="term" value="F:hyaluronic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:1905590">
        <property type="term" value="P:fibronectin fibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030212">
        <property type="term" value="P:hyaluronan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050728">
        <property type="term" value="P:negative regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0090024">
        <property type="term" value="P:negative regulation of neutrophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045668">
        <property type="term" value="P:negative regulation of osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045671">
        <property type="term" value="P:negative regulation of osteoclast differentiation"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0001550">
        <property type="term" value="P:ovarian cumulus expansion"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903911">
        <property type="term" value="P:positive regulation of receptor clustering"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Hyaluronan proteoglycan link protein 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
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    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
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    <dbReference type="InterPro" id="IPR000538">
        <property type="entry name" value="Link_dom"/>
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    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
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    <dbReference type="InterPro" id="IPR052129">
        <property type="entry name" value="Spermadhesin-Link_domain"/>
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        <property type="entry name" value="CUBILIN-LIKE PROTEIN"/>
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    <dbReference type="PANTHER" id="PTHR46908:SF4">
        <property type="entry name" value="TUMOR NECROSIS FACTOR-INDUCIBLE GENE 6 PROTEIN"/>
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    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
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        <property type="entry name" value="Xlink"/>
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    <dbReference type="PRINTS" id="PR01265">
        <property type="entry name" value="LINKMODULE"/>
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        <property type="entry name" value="LINK"/>
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        <property type="entry name" value="C-type lectin-like"/>
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        <property type="entry name" value="LINK_2"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
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    <feature type="chain" id="PRO_5034428418" description="Tumor necrosis factor-inducible gene 6 protein" evidence="10">
        <location>
            <begin position="18"/>
            <end position="277"/>
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        <location>
            <begin position="36"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="11">
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            <begin position="135"/>
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            <begin position="58"/>
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    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00022801"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00064571"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00069597"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00077610"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00081068"/>
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        <source>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="277" mass="31116" checksum="20BE399D972CEF08" modified="2022-01-19" version="1">MIILIYVLVLLWEEAHGWGFKNGIFHNSIWLEQAAGVYHREARSGKYKLTYAEAKAVCEYEGGHLATYKQLEAARKIGFHVCAAGWMAKGRVGYPIVKPGPNCGFGKTGIIDYGIRLNRSERWDAYCYNPHAKECGGIFTDTKRIFKSPGFPNEYDDNQVCYWHIRLKYGQRIHLSFLDFDLEDDPGCLADYVEIYDSYDDVHGFVGRYCGDELPEDIISTGNVMTLKFLSDASVTAGGFQIKYVAVDPPSKPSQGKNTSTTSTGNKSFLAGRFTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N0D1</accession>
    <name>A0A8C4N0D1_EQUAS</name>
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        <recommendedName>
            <fullName evidence="12">Small ribosomal subunit protein uS13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">40S ribosomal protein S18</fullName>
        </alternativeName>
    </protein>
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        <name evidence="16" type="primary">RPS18</name>
    </gene>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the small ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014701814.1">
        <property type="nucleotide sequence ID" value="XM_014846328.3"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A8C4N0D1"/>
    <dbReference type="Ensembl" id="ENSEAST00005033245.2">
        <property type="protein sequence ID" value="ENSEASP00005030584.1"/>
        <property type="gene ID" value="ENSEASG00005020817.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106834600"/>
    <dbReference type="KEGG" id="eai:106834600"/>
    <dbReference type="CTD" id="6222"/>
    <dbReference type="GeneTree" id="ENSGT00390000012691"/>
    <dbReference type="OMA" id="SYKGVRH"/>
    <dbReference type="OrthoDB" id="1702480at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.50:FF:000002">
        <property type="entry name" value="40S ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.910.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="entry name" value="30s ribosomal protein s13, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_uS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="Rbsml_uS13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_uS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_uS13_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003140">
        <property type="entry name" value="PRK04053.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S13/40S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF42">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 15" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00035468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="152" mass="17719" checksum="4DAF0662C3F37F22" modified="2022-01-19" version="1">MSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELTEDEVERVITIMQNPRQYKIPDWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PFG2</accession>
    <name>A0A8C4PFG2_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Chromatin target of PRMT1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CHTOP</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PI52"/>
    <dbReference type="Ensembl" id="ENSEAST00005007416.2">
        <property type="protein sequence ID" value="ENSEASP00005006786.2"/>
        <property type="gene ID" value="ENSEASG00005034777.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002732"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.1000:FF:000008">
        <property type="entry name" value="40S ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028333">
        <property type="entry name" value="Ribosomal_uS17_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_uS17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032440">
        <property type="entry name" value="Ribosomal_uS17_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006345">
        <property type="entry name" value="PRK08572.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03630">
        <property type="entry name" value="uS17_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF52">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16205">
        <property type="entry name" value="Ribosomal_S17_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0164">Citrullination</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 16" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="9 16" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS17 N-terminal" evidence="18">
        <location>
            <begin position="63"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00035164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF16205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006786.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="216" mass="24496" checksum="40285881E7B436BB" modified="2023-09-13" version="2">GPPVNQSGAQGTAPNPAAPPPFVTSSLAPAQPIRKPGRRAAFLFFRRPGRWRTFSSPAPSSSQTERAYQKQPTIFQNKKRVLLGETAKEKLPRYYKNIGLGFKTPKEAIEGTYIDKKCPFTGNVSIRGRILSGVVTKMKMQRTIVIRRDYLHYIRKYNRFEKRHKNMSVHLSPCFRDVQIGDIVTVGECRPLSKTVRFNVLKVTKAAGTKKQFQKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PIW1</accession>
    <name>A0A8C4PIW1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Zinc finger protein 697</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ZNF697</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">RNA-interacting protein with a high number of miRNA targets. Acts as a damage-induced regulator of muscle remodeling by mediating the interferon gamma response in muscle cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PIW1"/>
    <dbReference type="Ensembl" id="ENSEAST00005009720.2">
        <property type="protein sequence ID" value="ENSEASP00005008920.2"/>
        <property type="gene ID" value="ENSEASG00005006409.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00820000127150"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000711">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000862">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000931">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001035">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001076">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001259">
        <property type="entry name" value="Zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000290">
        <property type="entry name" value="Zinc finger protein 697 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000320">
        <property type="entry name" value="Zinc finger protein 777"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381:SF467">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24381">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="194"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="266"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="294"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="322"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="358"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="442"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="470"/>
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        </location>
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    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="498"/>
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    </feature>
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        <location>
            <begin position="526"/>
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    </feature>
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        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="112"/>
            <end position="146"/>
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        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="45"/>
            <end position="55"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="67"/>
            <end position="76"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="85"/>
            <end position="99"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068327"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093286"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008920.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="550" mass="60716" checksum="A3402BE709F46AC7" modified="2023-09-13" version="2">MEQEDKQGLCEAQDSADTGMGSDFENLEDREEDPEESGTGSNPQDADKREGHPEQEMASSPEDEALREDSEERELVSDTCTDGLLSEEEGALPEEEDDQPGVAEMAMFPGLSESDSISRSPPEEEEESAGDNRLEEEAAQPSRAVLPWRRHLSLGTRHRGDKPAHRRFRRLHHPMAVDLGELDSLMASIMDAPTICPDCGESFSPGAAFLQHQRIHCLAEAAAAASLETFGFAGDCGAVVGMMGVGVAGGFGAGPALARPPREKPFRCGECGKGFSRNTYLTNHLRLHTGERPNLCADCGKSFSWRADLLKHRRLHTGEKPYPCPECGEAFSLSSHLLSHRRAHAAASGAGAAALRPFACGECGKGFVRRSHLANHQRIHTGEKPHGCGECGKRFSWRSDLVKHQRVHTGEKPYMCSECGETFSVSSHLFTHKRTHSGERPYVCRECGKGFGRNSHLVNHLRVHTGEKPFRCGQCEKRFSDFSTLTQHQRTHTGEKPYTCIECGKSFIQSSHLIRHRRIHTGNKPHKCAGCGKGFRYKTHLAQHQKLHLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PPK7</accession>
    <name>A0A8C4PPK7_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ATPase family AAA domain containing 3A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ATAD3A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
            <location evidence="2">Mitochondrion nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PPK7"/>
    <dbReference type="Ensembl" id="ENSEAST00005022978.2">
        <property type="protein sequence ID" value="ENSEASP00005021164.2"/>
        <property type="gene ID" value="ENSEASG00005014502.2"/>
    </dbReference>
    <dbReference type="KEGG" id="eai:106845231"/>
    <dbReference type="GeneTree" id="ENSGT00730000111059"/>
    <dbReference type="OMA" id="HKSITGG"/>
    <dbReference type="OrthoDB" id="199596at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19512">
        <property type="entry name" value="RecA-like_ATAD3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000470">
        <property type="entry name" value="ATPase family, AAA domain containing 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021911">
        <property type="entry name" value="ATAD3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23075:SF0">
        <property type="entry name" value="ATPASE FAMILY AAA DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23075">
        <property type="entry name" value="PUTATIVE ATP-ASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12037">
        <property type="entry name" value="ATAD3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="10" id="KW-1135">Mitochondrion nucleoid</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="343"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="85"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="188"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005021164.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="591" mass="66665" checksum="0BBA11F7D8C6CC4D" modified="2023-09-13" version="2">MSWLFGIKGPRGEGAGPPLSLPPAQPGAEGGGDRGAGDRPAPKDKWSNFDPTGLERAAKAARELEHSRHAKEALSLAQMQEQTLQLEQQSKLKEYEAAVEQLKSEQIRVQAEERRKTLNEETRQHQARAQYQDKLARQRYEDQLRQQQLLNEENLRKQEESVQKQEAIRRATVEREMELRHKNEMLRVEAEARARAKAERENADIIREQIRLKAAEHRQTILESIRTAGTLFGEGFRAFVTDWDKVTATVAGLTLLAVGVYSAKNATSVAGRYIEARLGKPSLVRETSRITVLEALRHPLQVSRRLLSKPQDALEGVVLSPSLEARVRDIAIATRNTKKNRSLYRNILMYGPPGTGKTLFAKKLALHSGMDYAIMTGGDVAPMGRDGVTAVHKVFDWASTSRRGLLLFVDEADAFLRKRATEKISEDLRATLNAFLHRTGQHSSKFMLVLASNQPEQFDWAINDRIDEMVSFDLPQLEERERLVRMYFDKHVLKPATEGKQRLKLAQFDYGKKCSEIARLTEGMSGREISQLAVAWQAMAYASEDGVLTEAMMDARVQDAIQQHQQKMQWLKAERAGLEGSQTLLPSARAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PTQ1</accession>
    <name>A0A8C4PTQ1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zinc finger protein 583</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ZNF583</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044615196.1">
        <property type="nucleotide sequence ID" value="XM_044759261.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PTQ1"/>
    <dbReference type="Ensembl" id="ENSEAST00005032690.2">
        <property type="protein sequence ID" value="ENSEASP00005030077.1"/>
        <property type="gene ID" value="ENSEASG00005020474.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106823985"/>
    <dbReference type="CTD" id="147949"/>
    <dbReference type="GeneTree" id="ENSGT00940000161925"/>
    <dbReference type="OMA" id="EYSKSWQ"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000224">
        <property type="entry name" value="Zinc finger protein 329"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000101">
        <property type="entry name" value="zinc finger protein 436 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000238">
        <property type="entry name" value="Zinc finger protein 485"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000200">
        <property type="entry name" value="zinc finger protein 510 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000737">
        <property type="entry name" value="Zinc finger protein 565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000826">
        <property type="entry name" value="Zinc finger protein 570"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 208-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="16">
        <location>
            <begin position="6"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="212"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="240"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="268"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="296"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="324"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="352"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="380"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="408"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="436"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="464"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="492"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="520"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="180"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="180"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="569" mass="65794" checksum="BD30CD9D5A188E78" modified="2022-01-19" version="1">MSKDLVTFGDVAVDFSQEEWQWLNSAQRNLYRKVMLENYRSLVSLGVSVSKPDVISLLEQGKEPWMVKKEGTRGTCPDWEYALKNNEFSSEQVIYEESSRVATMGRSHLSYSLDCTSWREDCKSEDWFKKQSGSQKVHSSQLVITHKSMPSKNPSTEYTKSWQTFHQDAIFAIKQSFPTKERVRKHEPQKRSYQKKPVQMKHKKVYVEKKLLKCNECEKVFNQSSSLTLHQRIHTGEKPYACVECGKTFSQSANLAQHKRIHTGEKPYECKECRKAFSQNAHLAQHQRVHTGEKPYQCKECKKAFSQIAHLTQHQRVHTGERPFECIECGKAFSNGSFLAQHQRIHTGEKPYVCNVCAKAFSHRGYLIVHQRIHTGERPYECKECRKAFSQYAHLAQHQRVHTGEKPYECKVCRKAFSQIAYLDQHQRVHTGEKPYECTECGKAFSNSSSLAQHQRSHTGEKPYMCKECRKTFSQNAGLAQHQRIHTGEKPYACNVCGKAFSYSGSLTLHQRIHTGERPYECKDCRKSFRQRAHLAHHERIHTMESFLTLSSPSPSMSSQLPRPLGFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I5M2</accession>
    <name>A0A9L0I5M2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Transcription factor A, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">TFAM</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Monomer; binds DNA as a monomer. Homodimer. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT. In this complex TFAM recruits POLRMT to the promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Upon metabolic stress, forms a complex composed of FOXO3, SIRT3, TFAM and POLRMT. Interacts with TFB1M and TFB2M. Interacts with CLPX; this enhances DNA-binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
            <location evidence="1">Mitochondrion nucleoid</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014708449.1">
        <property type="nucleotide sequence ID" value="XM_014852963.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0I5M2"/>
    <dbReference type="Ensembl" id="ENSEAST00005074712.1">
        <property type="protein sequence ID" value="ENSEASP00005035434.1"/>
        <property type="gene ID" value="ENSEASG00005032581.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106838734"/>
    <dbReference type="KEGG" id="eai:106838734"/>
    <dbReference type="CTD" id="7019"/>
    <dbReference type="GeneTree" id="ENSGT00440000039001"/>
    <dbReference type="OMA" id="YMQLAED"/>
    <dbReference type="OrthoDB" id="5550281at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
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        <location>
            <begin position="20"/>
            <end position="395"/>
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            <begin position="280"/>
            <end position="303"/>
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            <begin position="143"/>
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            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005034909.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="395" mass="43747" checksum="7E38FB3E3F91F2F9" modified="2023-09-13" version="1">MGLLLGLLLLGHLTVVTYGLPILEAPESVTGPWKGDVNIPCTYSPLQGYTQVLVKWLVQRGSDPVTIFLRDSSGDHIQQAKYRGRLHVNHGVPGDVSLQLNTLEMDDRSHYMCEVTWHNPDGNQVVRDKIIELRVQKLSVSKPTVTTGSGYGFTVPQGMRISLQCQARGSPPISYVWYKEQINNQEPIKVAALSTLLFKPAVVANSGSYFCTAKGRVGSEQRSDIVKLVVKDSSKILKTKTEAPTTMQSPLEVNPSWSWTTEVDGYLGETSAGSGKGLPMFAIILIISLCCMVVFTMASIMLCRKTSQQEHVYEVARVHAREANDSGETMRVTISASGCSREEPASWTLGNEYSEERCLSQESQIITRINNDYTHPLHTVPPDYELLATNRKRLC</sequence>
</entry>
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    <accession>A0A9L0ICX3</accession>
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        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
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        <name evidence="15" type="primary">LOC106836040</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="gene ID" value="ENSEASG00005025300.1"/>
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    <dbReference type="KEGG" id="eai:106836040"/>
    <dbReference type="GeneTree" id="ENSGT01010000222320"/>
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    <dbReference type="GO" id="GO:0005886">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15405">
        <property type="entry name" value="7tmA_OR8B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000004">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
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    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
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        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="90"/>
            <end position="119"/>
        </location>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005034897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="310" mass="34416" checksum="093A388D8F59EC64" modified="2023-09-13" version="1">MTAENSSVTEFILAGLTDQPGLQIPLFFLFLGFYMVTVVGNLTLITLIGLNSHLHTPMYFFLFNLSLIDFCYSTTITPKMLKSFVSKQNIILHAGCVTQLFFFCFLVISESFILSAMAYDRYVAICKPLVYTVTMSPQVCLRLVLGVYVMGFSGALAHTGSIASLTFCADNLVNHFMCDIFPLLELSCNSTYVHILVVFIFVTTDIGMPIVTIFISYGLILSSILHIHSTEGRSKAFSTCGSHIIVVSLFFGSGAFVYLKPPSILPLDQGKVFSLFYTIVVPMLNPLIYSLRNKDVKVALRKTLGRKIFS</sequence>
</entry>
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    <accession>A0A9L0IEA7</accession>
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        <submittedName>
            <fullName evidence="15">SLAM family member 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SLAMF6</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Liu"/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </evidence>
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        <source>
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        </source>
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    <accession>A0A9L0IFS5</accession>
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    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Protein-lysine methyltransferase that selectively mono-, di- and trimethylates 'Lys-165' of the translation elongation factors EEF1A1 and EEF1A2 in an aminoacyl-tRNA and GTP-dependent manner. EEF1A1 methylation by EEF1AKMT3 is dynamic as well as inducible by stress conditions, such as ER-stress, and plays a regulatory role on mRNA translation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:54192"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13826"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:54193"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-lysyl-[protein] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51736"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:13053"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:51737"/>
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    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6),N(6)-dimethyl-L-lysyl-[protein] + S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
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    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(6)-methyl-L-lysyl-[protein] + S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
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    <comment type="subunit">
        <text evidence="13">Interacts with members of the heat shock protein 70 and 90 families and of the TCP-1 chaperonin family, as well as with HSPD1, STIP1 and tubulin; at least some of these proteins may be methylation substrates.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
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            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="7">Belongs to the methyltransferase superfamily. METTL21 family.</text>
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        <property type="nucleotide sequence ID" value="XM_014844042.3"/>
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        <property type="entry name" value="EEF1A LYSINE METHYLTRANSFERASE 3"/>
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    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="Y."/>
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                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Liu"/>
                <person name="W."/>
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                <person name="J."/>
                <person name="Pan"/>
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                <person name="G."/>
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                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
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                <person name="X."/>
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                <person name="Yue"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="match status" value="1"/>
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            <begin position="7"/>
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        </location>
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        <location>
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        </location>
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        <location>
            <begin position="246"/>
            <end position="259"/>
        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF14916"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005036728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="259" mass="28779" checksum="8F78CED02EFC1328" modified="2023-09-13" version="1">MDTVSLEHQIQSVQRHISFLKKEQMALLRDLHLEILRLQKRCSELTHDLELREAQSHQQEAASRELESKCRALESQLAARAAANAELRREVAQREALVSALRCSLRAEERRFLDELRRRSHRTTVLGTELQKHTEAAAYLSCQLHAVRQRLQTPRPGPGAAAEPRPRRRAQRARRLPAPAAAAAEAAAKGPGRDWAAWDRAAGALDDADAMPDPALFLYARRPPRPSGRSPRQPPPQEPPDEAGPQPAPSPPGAPGDQE</sequence>
</entry>
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    <accession>A0A9L0IKR3</accession>
    <name>A0A9L0IKR3_EQUAS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="synonym">Equus africanus asinus</name>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="J."/>
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                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IKR3"/>
    <dbReference type="Ensembl" id="ENSEAST00005083279.1">
        <property type="protein sequence ID" value="ENSEASP00005041530.1"/>
        <property type="gene ID" value="ENSEASG00005030322.1"/>
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        <property type="component" value="Chromosome 26"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33426">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005041530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="207" mass="23476" checksum="7AD38165CDFB9852" modified="2023-09-13" version="1">MRPFSSMRSQMITEAGASSIRFPTFPALIRPFSSVNSLMITEDGAIGKRFPTFTAHIRLFSRVNSLVYDELPPILKRFPTFITLVRPFSSVNYLMITELGAKDKTFPTFTALIRPFSTMNPLMITEARAHGKRFPAFTALIRPFSCVSTLMITKAGPRSKRFPTFLAFIRPFSCVRSLMITEGRATSKRFHTDTTLVRLFSRMNSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0ILJ7</accession>
    <name>A0A9L0ILJ7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Bactericidal permeability-increasing protein</fullName>
            <shortName evidence="4">BPI</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106835090</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer. Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">The N- and C-terminal barrels adopt an identical fold despite having only 13% of conserved residues.</text>
    </comment>
    <comment type="domain">
        <text evidence="4">The N-terminal region may be exposed to the interior of the granule, whereas the C-terminal portion may be embedded in the membrane. During phagocytosis and degranulation, proteases may be released and activated and cleave BPI at the junction of the N- and C-terminal portions of the molecule, providing controlled release of the N-terminal antibacterial fragment when bacteria are ingested.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0ILJ7"/>
    <dbReference type="Ensembl" id="ENSEAST00005049377.1">
        <property type="protein sequence ID" value="ENSEASP00005041763.1"/>
        <property type="gene ID" value="ENSEASG00005035432.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286994"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
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    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001530">
        <property type="term" value="F:lipopolysaccharide binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006953">
        <property type="term" value="P:acute-phase response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050829">
        <property type="term" value="P:defense response to Gram-negative bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031663">
        <property type="term" value="P:lipopolysaccharide-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002281">
        <property type="term" value="P:macrophage activation involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043032">
        <property type="term" value="P:positive regulation of macrophage activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00025">
        <property type="entry name" value="BPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.15.10.10:FF:000001">
        <property type="entry name" value="phospholipid transfer protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.20.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017943">
        <property type="entry name" value="Bactericidal_perm-incr_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032942">
        <property type="entry name" value="BPI/LBP/Plunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001124">
        <property type="entry name" value="Lipid-bd_serum_glycop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017942">
        <property type="entry name" value="Lipid-bd_serum_glycop_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10504">
        <property type="entry name" value="BACTERICIDAL PERMEABILITY-INCREASING BPI PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10504:SF76">
        <property type="entry name" value="BACTERICIDAL PERMEABILITY-INCREASING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01273">
        <property type="entry name" value="LBP_BPI_CETP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02886">
        <property type="entry name" value="LBP_BPI_CETP_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00328">
        <property type="entry name" value="BPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00329">
        <property type="entry name" value="BPI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55394">
        <property type="entry name" value="Bactericidal permeability-increasing protein, BPI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0044">Antibiotic</keyword>
    <keyword evidence="4" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="2 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 5" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040376475" description="Bactericidal permeability-increasing protein" evidence="5">
        <location>
            <begin position="25"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="Lipid-binding serum glycoprotein N-terminal" evidence="6">
        <location>
            <begin position="33"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Lipid-binding serum glycoprotein C-terminal" evidence="7">
        <location>
            <begin position="271"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005041763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="457" mass="50565" checksum="5F5A4B311300EA26" modified="2023-09-13" version="1">MMARLYCVVVVLLLLAEVARFGEGASNPGLVARITRKGLKYVHERVAAALQKELSAIEVPDFLGSFQVGWFGRVSYESNGLRIQSLELRNSDLSLRPGRGVRASLFNNYVSVSGNWKVTTAFVILNGTFGLSVDGISISVSLNLGKDPSGRPTASVVHCDSSIDGIDINISGPLSRIQNFFHGIMEIYFKNILEQKICEMVRMSTTSRLEPYLRTLPVTSMIDQVAGIDYSLVGAPQVTSQCLDTPFKGEFFGRSWHSPVPFDAPPIRLPQEHDRMIYFAVSDFVFNTASRVYHQAGRMKFIIQNEHLKSLTKLANNESAKKWRKQYLNSSCQTSKSTSLTATVSCFASSFLLLLLQIPLDSSIALNTNSFRALIPQLAKLYPNMQLEFETSPESAPFLMFTPGNVTLLPVMDIQTFALPPNSSDRKPLFQLRVRTSISATIGVTSSRIIASLTSVR</sequence>
</entry>
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    <accession>A0A9L0IMF6</accession>
    <name>A0A9L0IMF6_EQUAS</name>
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            <fullName evidence="7">Transmembrane 4 L six family member 20</fullName>
        </submittedName>
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        <name evidence="7" type="primary">TM4SF20</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
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            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the L6 tetraspanin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014691446.3">
        <property type="nucleotide sequence ID" value="XM_014835960.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IMF6"/>
    <dbReference type="Ensembl" id="ENSEAST00005043731.1">
        <property type="protein sequence ID" value="ENSEASP00005042131.1"/>
        <property type="gene ID" value="ENSEASG00005028247.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106827862"/>
    <dbReference type="KEGG" id="eai:106827862"/>
    <dbReference type="CTD" id="79853"/>
    <dbReference type="GeneTree" id="ENSGT01030000234590"/>
    <dbReference type="OMA" id="LEGPLMC"/>
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        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045861">
        <property type="term" value="P:negative regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008661">
        <property type="entry name" value="L6_membrane"/>
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    <dbReference type="PANTHER" id="PTHR14198">
        <property type="entry name" value="TRANSMEMBRANE 4 L6 FAMILY MEMBER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14198:SF17">
        <property type="entry name" value="TRANSMEMBRANE 4 L6 FAMILY MEMBER 20"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF05805">
        <property type="entry name" value="L6_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="184"/>
            <end position="217"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006193"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005042131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="227" mass="24741" checksum="3AA7E09EBBF0507B" modified="2023-09-13" version="1">MTCCEGWTSCNGFSLLVLLVLGVILNAIPLIVSLVDADQVFQNPISCFEWWFPGIIGAGVMVIPATSMSLAARKRACCNSKVGMFLSSLLNIITVIGAVYCMLVSIQALAEGPLICNSQGNSTSSCEFSFKNLRDIHLESFNVQWFFNESCVTSTGFTNPTNNDIMASNWGKNSLNSTENKYRIIHFSVFLGLLLVGILELLFGLSQIVIGFLGCLCGVSKRRSRIV</sequence>
</entry>
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            <fullName evidence="4">Uncharacterized protein</fullName>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
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                <person name="J."/>
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                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IN31"/>
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        <property type="gene ID" value="ENSEASG00005007044.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013989"/>
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        <property type="component" value="Chromosome X"/>
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    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="PLEKHB1/2"/>
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        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14309:SF8">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY B MEMBER 2"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005042366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="136" mass="14855" checksum="B60B8FB1454C5B14" modified="2023-09-13" version="1">DGIVKSGWLLRQRRECQDVQPPDGKPKDCMLQILRRDGRTISLCSESTEDCLAQKFMLQDSRINTMAVASSPPPYTAYAAPTPEQAYGYGPYGGPANQVIICERYGDNDSDLTVGMLAGAATGMALGSLFWVFWSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IQU2</accession>
    <name>A0A9L0IQU2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Serine/threonine-protein kinase 3</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Mammalian STE20-like protein kinase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">STE20-like kinase MST2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">STK3</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:17990"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:46609"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="21">Homodimer; mediated via the coiled-coil region. Interacts with NORE1, which inhibits autoactivation. Interacts with and stabilizes SAV1. Interacts with RAF1, which prevents dimerization and phosphorylation. Interacts with RASSF1. Interacts (via SARAH domain) with isoform 1 of NEK2. Interacts with ESR1 only in the presence of SAV1. Interacts with PKB/AKT1. Forms a tripartite complex with MOBKL1B and STK38. Interacts with RASSF2 (via SARAH domain). Interacts with DLG5 (via PDZ domain 3). Interacts with LATS1; this interaction is inhibited in the presence of DLG5. Interacts with MARK3 in the presence of DLG5. Interacts with RASSF5; this interaction inhibits STK3 autoactivation through heterodimerization. Interacts (when phosphorylated) with SLMAP (via FHA domain); the interaction associates STK3 with the STRIPAK complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0IQU2"/>
    <dbReference type="Ensembl" id="ENSEAST00005073036.1">
        <property type="protein sequence ID" value="ENSEASP00005040146.1"/>
        <property type="gene ID" value="ENSEASG00005003082.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154984"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140537">
        <property type="term" value="F:transcription regulator activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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</entry>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Negative regulator of epidermal growth factor receptor (EGFR) signaling. Acts by promoting EGFR degradation in endosomes when not monoubiquitinated.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with TRIM3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lst-2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IR46"/>
    <dbReference type="Ensembl" id="ENSEAST00005080819.1">
        <property type="protein sequence ID" value="ENSEASP00005042583.1"/>
        <property type="gene ID" value="ENSEASG00005028915.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157217"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15731">
        <property type="entry name" value="FYVE_LST2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000092">
        <property type="entry name" value="Lateral signaling target protein 2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043269">
        <property type="entry name" value="FYVE_LST2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051118">
        <property type="entry name" value="LST-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46465">
        <property type="entry name" value="LATERAL SIGNALING TARGET PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46465:SF3">
        <property type="entry name" value="LATERAL SIGNALING TARGET PROTEIN 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="18">
        <location>
            <begin position="131"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00083237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005042583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="201" mass="22199" checksum="3E7B7D1FDFC3D310" modified="2023-09-13" version="1">MRARSGSTAGSSSSDKTGLDAVPAATREKIRSRFHGSHDLIHRLFVCISGVADQLQTNYASDLRSILKTLFEVMATKPETDDKEKLKKVTQTLRSAALEDCALCQETLSSSELATKTHDGDLEDPPEWVPDEACGFCTACKAPFTVIRRKHHCRSCGKIFCSRCSSHSAPLPRYGQVKPVRVCTHCYMFHVTPFYSDKAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0ISY3</accession>
    <name>A0A9L0ISY3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">aldehyde oxidase</fullName>
            <ecNumber evidence="5">1.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC106825581</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>an aldehyde + O2 + H2O = a carboxylate + H2O2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16829"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="EC" id="1.2.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>Mo-molybdopterin</name>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
        </cofactor>
        <text evidence="19">Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="19">Binds 2 [2Fe-2S] clusters.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the xanthine dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.3.1" evidence="5"/>
    <dbReference type="Ensembl" id="ENSEAST00005078895.1">
        <property type="protein sequence ID" value="ENSEASP00005040896.1"/>
        <property type="gene ID" value="ENSEASG00005027058.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183114"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004031">
        <property type="term" value="F:aldehyde oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.120:FF:000001">
        <property type="entry name" value="Aldehyde oxidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000015">
        <property type="entry name" value="Aldehyde oxidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.365.10:FF:000003">
        <property type="entry name" value="Aldehyde oxidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.50:FF:000001">
        <property type="entry name" value="Aldehyde oxidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.365.10:FF:000001">
        <property type="entry name" value="Xanthine dehydrogenase oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.365.10:FF:000004">
        <property type="entry name" value="Xanthine dehydrogenase oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.50:FF:000001">
        <property type="entry name" value="Xanthine dehydrogenase oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.43.10:FF:000001">
        <property type="entry name" value="Xanthine dehydrogenase/oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.465.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.120">
        <property type="entry name" value="[2Fe-2S]-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.50">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, a/b hammerhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.365.10">
        <property type="entry name" value="Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.50">
        <property type="entry name" value="CO dehydrogenase flavoprotein, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.43.10">
        <property type="entry name" value="Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002888">
        <property type="entry name" value="2Fe-2S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036884">
        <property type="entry name" value="2Fe-2S-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000674">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036856">
        <property type="entry name" value="Ald_Oxase/Xan_DH_a/b_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016208">
        <property type="entry name" value="Ald_Oxase/xanthine_DH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014313">
        <property type="entry name" value="Aldehyde_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008274">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046867">
        <property type="entry name" value="AldOxase/xan_DH_MoCoBD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037165">
        <property type="entry name" value="AldOxase/xan_DH_Mopterin-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005107">
        <property type="entry name" value="CO_DH_flav_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036683">
        <property type="entry name" value="CO_DH_flav_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016166">
        <property type="entry name" value="FAD-bd_PCMH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036318">
        <property type="entry name" value="FAD-bd_PCMH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016167">
        <property type="entry name" value="FAD-bd_PCMH_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016169">
        <property type="entry name" value="FAD-bd_PCMH_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002346">
        <property type="entry name" value="Mopterin_DH_FAD-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02969">
        <property type="entry name" value="mam_aldehyde_ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45444">
        <property type="entry name" value="XANTHINE DEHYDROGENASE"/>
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        <property type="entry name" value="Ald_Xan_dh_C"/>
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        <property type="entry name" value="CO_deh_flav_C"/>
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        <property type="entry name" value="Fer2"/>
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    <dbReference type="Pfam" id="PF01799">
        <property type="entry name" value="Fer2_2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF02738">
        <property type="entry name" value="MoCoBD_1"/>
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    <dbReference type="Pfam" id="PF20256">
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    <dbReference type="PIRSF" id="PIRSF000127">
        <property type="entry name" value="Xanthine_DH"/>
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    <dbReference type="SMART" id="SM01008">
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    </dbReference>
    <dbReference type="SMART" id="SM01092">
        <property type="entry name" value="CO_deh_flav_C"/>
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        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
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    <dbReference type="SUPFAM" id="SSF55447">
        <property type="entry name" value="CO dehydrogenase flavoprotein C-terminal domain-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF47741">
        <property type="entry name" value="CO dehydrogenase ISP C-domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54665">
        <property type="entry name" value="CO dehydrogenase molybdoprotein N-domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56176">
        <property type="entry name" value="FAD-binding/transporter-associated domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56003">
        <property type="entry name" value="Molybdenum cofactor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51387">
        <property type="entry name" value="FAD_PCMH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 19" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 18" id="KW-0274">FAD</keyword>
    <keyword evidence="8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 19" id="KW-0408">Iron</keyword>
    <keyword evidence="14 19" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="10 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 19" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="20">
        <location>
            <begin position="5"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding PCMH-type" evidence="21">
        <location>
            <begin position="236"/>
            <end position="418"/>
        </location>
    </feature>
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        <location>
            <position position="1269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
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            <label>1</label>
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        <location>
            <position position="49"/>
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        <location>
            <begin position="264"/>
            <end position="271"/>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="3">
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                <consortium name="Ensembl"/>
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        <property type="term" value="C:external side of plasma membrane"/>
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        <property type="entry name" value="Snake_toxin-like_sf"/>
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    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040348145" description="UPAR/Ly6 domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="2">
        <location>
            <begin position="19"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005044506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="124" mass="13723" checksum="79F7EE17715E94B5" modified="2023-09-13" version="1">MKGLLLLTLSSLVCWVSADIRCHSCYKVPVLGCVDRQSCRLEPGQQCLTTNVYLGKMRVFSNLRCGTPEEPCREAFYQTSHKLGLTYNTTCCNKDTCNSLAPRPTPALDLVLTSLAGLGLWLLH</sequence>
</entry>
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        <submittedName>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Laurasiatheria</taxon>
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            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
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                <person name="Ju"/>
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                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
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                <person name="Hou"/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
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                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IU65"/>
    <dbReference type="Ensembl" id="ENSEAST00005070748.1">
        <property type="protein sequence ID" value="ENSEASP00005040629.1"/>
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00980000198589"/>
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        <property type="component" value="Chromosome 26"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33426">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005040629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="173" mass="19533" checksum="C91CF70DEB520476" modified="2023-09-13" version="1">MDSKLLAYSKSLSTLLTFIGFLTKMNFLMYSKVWTTYEGFPTFLTFIELLSSVKSLMSSKVCTLPKGLPTCLTFIGFLTSVNSLMSSKLLMYSKGLPTFLTFIGLFTCMKSLMLSKFLEHRKGLPTFLTFIGFLTSMKFVMSNKVCTLSKGLPTFLTFIRFLTSMNSQMLTKC</sequence>
</entry>
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    <accession>A0A9L0IV82</accession>
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        <recommendedName>
            <fullName evidence="12">Gap junction protein</fullName>
        </recommendedName>
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    <gene>
        <name evidence="17" type="primary">GJA5</name>
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    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
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            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Fang"/>
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                <person name="Zhao"/>
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                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Zhuang"/>
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                <person name="Ju"/>
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                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
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                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
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                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 12">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 12">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 12">Cell membrane</location>
            <topology evidence="3 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the connexin family. Alpha-type (group II) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014722549.1">
        <property type="nucleotide sequence ID" value="XM_014867063.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014722551.1">
        <property type="nucleotide sequence ID" value="XM_014867065.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044614210.1">
        <property type="nucleotide sequence ID" value="XM_044758275.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070353790.1">
        <property type="nucleotide sequence ID" value="XM_070497689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IV82"/>
    <dbReference type="SMR" id="A0A9L0IV82"/>
    <dbReference type="Ensembl" id="ENSEAST00005042290.1">
        <property type="protein sequence ID" value="ENSEASP00005043534.1"/>
        <property type="gene ID" value="ENSEASG00005034147.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005047207.1">
        <property type="protein sequence ID" value="ENSEASP00005063054.1"/>
        <property type="gene ID" value="ENSEASG00005034147.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005052076.1">
        <property type="protein sequence ID" value="ENSEASP00005044794.1"/>
        <property type="gene ID" value="ENSEASG00005034147.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005057177.1">
        <property type="protein sequence ID" value="ENSEASP00005059193.1"/>
        <property type="gene ID" value="ENSEASG00005034147.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005063442.1">
        <property type="protein sequence ID" value="ENSEASP00005034606.1"/>
        <property type="gene ID" value="ENSEASG00005034147.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106847659"/>
    <dbReference type="KEGG" id="eai:106847659"/>
    <dbReference type="CTD" id="2702"/>
    <dbReference type="GeneTree" id="ENSGT01150000286930"/>
    <dbReference type="OMA" id="FIHIHYG"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014704">
        <property type="term" value="C:intercalated disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086076">
        <property type="term" value="F:gap junction channel activity involved in atrial cardiac muscle cell-AV node cell electrical coupling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086077">
        <property type="term" value="F:gap junction channel activity involved in AV node cell-bundle of His cell electrical coupling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086078">
        <property type="term" value="F:gap junction channel activity involved in bundle of His cell-Purkinje myocyte electrical coupling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055077">
        <property type="term" value="F:gap junction hemi-channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048844">
        <property type="term" value="P:artery morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060413">
        <property type="term" value="P:atrial septum morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003294">
        <property type="term" value="P:atrial ventricular junction remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097746">
        <property type="term" value="P:blood vessel diameter maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003161">
        <property type="term" value="P:cardiac conduction system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035050">
        <property type="term" value="P:embryonic heart tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030326">
        <property type="term" value="P:embryonic limb morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035922">
        <property type="term" value="P:foramen ovale closure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016264">
        <property type="term" value="P:gap junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003174">
        <property type="term" value="P:mitral valve development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003151">
        <property type="term" value="P:outflow tract morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003193">
        <property type="term" value="P:pulmonary valve formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098910">
        <property type="term" value="P:regulation of atrial cardiac muscle cell action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060371">
        <property type="term" value="P:regulation of atrial cardiac muscle cell membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098904">
        <property type="term" value="P:regulation of AV node cell action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098905">
        <property type="term" value="P:regulation of bundle of His cell action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086091">
        <property type="term" value="P:regulation of heart rate by cardiac conduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900825">
        <property type="term" value="P:regulation of membrane depolarization during cardiac muscle cell action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098906">
        <property type="term" value="P:regulation of Purkinje myocyte action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900133">
        <property type="term" value="P:regulation of renin secretion into blood stream"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060373">
        <property type="term" value="P:regulation of ventricular cardiac muscle cell membrane depolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060307">
        <property type="term" value="P:regulation of ventricular cardiac muscle cell membrane repolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003071">
        <property type="term" value="P:renal system process involved in regulation of systemic arterial blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086015">
        <property type="term" value="P:SA node cell action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003284">
        <property type="term" value="P:septum primum development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086005">
        <property type="term" value="P:ventricular cardiac muscle cell action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060412">
        <property type="term" value="P:ventricular septum morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.80:FF:000001">
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        <property type="entry name" value="Connexin"/>
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    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
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    <dbReference type="InterPro" id="IPR031862">
        <property type="entry name" value="Cx40_C"/>
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    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF13">
        <property type="entry name" value="GAP JUNCTION ALPHA-5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16791">
        <property type="entry name" value="Connexin40_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01135">
        <property type="entry name" value="CONNEXINA5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM01089">
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    <dbReference type="PROSITE" id="PS00407">
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    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0303">Gap junction</keyword>
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            <end position="41"/>
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            <begin position="43"/>
            <end position="76"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005044794.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="358" mass="40748" checksum="3F698E133CA0846A" modified="2023-09-13" version="1">MGDWSYLAEFLEEVHKHSTEIGKVWLTVLFIFRILVLVTAAESSWGDEQADFLCDTLQPGCENVCYDQAFPISHIRYWVLQVIFVSTPSLVYLFHTMHRVRMQEKQRQREAERAKHVQGAGSYEYPVAEKTALSCWEEGNEKIVLQGTLLNTYVCSILIRTTMEVAFIVGQYLLYGIFLDTLHVCRRSPCPHPVNCYVSRPTEKNVFIVFMLAVAGLSLFLSLVELYHLGWKRIRQRFARSRQGMAECQLPGPSAGIVQKCTPPLDFNQCLENGPGGKFFNPFSNKMASQQNIDNLATEQVRDQEQIPGEGFIHIRYAQKPEVPNGVSPGHRLPHCYQNDKPRLSKASSKARSDDLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IWK2</accession>
    <name>A0A9L0IWK2_EQUAS</name>
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        <recommendedName>
            <fullName evidence="9">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
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                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="G."/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
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                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="ENSEASG00005026567.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 8"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
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        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
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    <feature type="chain" id="PRO_5040304176" description="Amino acid permease/ SLC12A domain-containing protein" evidence="8">
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            <begin position="34"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
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            <begin position="67"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="224"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005042142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="295" mass="30855" checksum="FD3B747660480B31" modified="2023-09-13" version="1">MWASGRCPFWPGTQTSWLLASYFCSWLSSPVEVVSLPGSTTSSLPSVSSSSSSSSSWGLSWPTPHNWSGEEGGFAPFGFSGIMTGAATCFYAFAGFGAIAASSVEARNPKRTVPMALAISVGVVAGAYILVSTVLTLMVPWHNLDPDSALADAFHQQGHSWAAFIVVAGAICGSHGAPDPCPEHPPQHLPHATPELRDLAGLLHLVVDRTRGIFWLRHQAQQGEPAGAAQVDGHTQQPEDGASPAAPQPGTGPRAWPHGGANQSMRTTGASLPSPTFSGGQKNWQSLYLGQLGFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J3V6</accession>
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        <submittedName>
            <fullName evidence="19">ADAM metallopeptidase domain 15</fullName>
        </submittedName>
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    <gene>
        <name evidence="19" type="primary">ADAM15</name>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
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                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="entry name" value="disintegrin and metalloproteinase domain-containing protein 11"/>
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        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
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        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
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        <property type="entry name" value="ADAM_Cys-rich"/>
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        <property type="entry name" value="Disintegrin_dom_sf"/>
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        <property type="entry name" value="EGF"/>
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        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
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        <property type="entry name" value="Peptidase_M12B"/>
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        <property type="entry name" value="Peptidase_M12B_N"/>
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    <dbReference type="InterPro" id="IPR034027">
        <property type="entry name" value="Reprolysin_adamalysin"/>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Associates with TRAF1 and TRAF2, and probably also with TRAF3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014688664.1">
        <property type="nucleotide sequence ID" value="XM_014833178.3"/>
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    <dbReference type="SMR" id="A0A9L0J4E6"/>
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        <property type="protein sequence ID" value="ENSEASP00005048151.1"/>
        <property type="gene ID" value="ENSEASG00005028268.1"/>
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    <dbReference type="GeneID" id="106826046"/>
    <dbReference type="KEGG" id="eai:106826046"/>
    <dbReference type="CTD" id="51330"/>
    <dbReference type="GeneTree" id="ENSGT00390000001948"/>
    <dbReference type="OMA" id="HSDFCRG"/>
    <dbReference type="OrthoDB" id="9010896at2759"/>
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        <property type="component" value="Chromosome 14"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0097191">
        <property type="term" value="P:extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045773">
        <property type="term" value="P:positive regulation of axon extension"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:2001238">
        <property type="term" value="P:positive regulation of extrinsic apoptotic signaling pathway"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045765">
        <property type="term" value="P:regulation of angiogenesis"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0061041">
        <property type="term" value="P:regulation of wound healing"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:substrate-dependent cell migration, cell attachment to substrate"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="CDD" id="cd13413">
        <property type="entry name" value="TNFRSF12A"/>
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        <property type="entry name" value="tumor necrosis factor receptor superfamily member 12A"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR SUPERFAMILY MEMBER 12A"/>
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            <begin position="28"/>
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    <sequence length="129" mass="13629" checksum="98861350B45BFE2B" modified="2023-09-13" version="1">MIPGPLRPRLRFLVLGLGLALLRAAAGEPVPGTTPCSRGSSWSADLDKCMDCASCPARPHSDFCLGCAAAPPASFRLLWPILGGALSLALVLGLLSGFLVWRRCRRREKFTTPIEETGGEGCPGVALIQ</sequence>
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        <recommendedName>
            <fullName evidence="6">HMG box domain-containing protein</fullName>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Nucleus</location>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0030182">
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Transcription factor SOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
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    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
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    <dbReference type="InterPro" id="IPR050140">
        <property type="entry name" value="SRY-related_HMG-box_TF-like"/>
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    <dbReference type="PANTHER" id="PTHR10270">
        <property type="entry name" value="SOX TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10270:SF113">
        <property type="entry name" value="TRANSCRIPTION FACTOR SOX-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="6">
        <location>
            <begin position="49"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="4">
        <location>
            <begin position="49"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
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    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="115"/>
            <end position="258"/>
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    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="274"/>
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    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="358"/>
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        <location>
            <begin position="143"/>
            <end position="156"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="170"/>
            <end position="180"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="193"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
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    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="300"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="372"/>
            <end position="381"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005044255.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="406" mass="45027" checksum="645B7DA1AC24201D" modified="2023-09-13" version="1">MVQQAESLEAESNLPREALDTEEGEFMACSPVALDESDPDWCKTASGHIKRPMNAFMVWSKIERRKIMEQSPDMHNAEISKRLGKRWKMLKDSEKIPFIREAERLRLKHMADYPDYKYRPRKKPKMDPSAKPSASQSPEKSAAGGGGGGAGGGAGGAKTSKGSSKKCSKLKAPAAPAGQRRQGGRGQGGPARGLRRRGRRLRARQPARGRRGRRRRGQDGQVRVPGRRRRRRRRRRRAAAADQAGGGRGRRRAAARAAPAAAGAAAAAAAETLQCRQSARQPHAEQLGRVPRGREPLRRGAGRRHLGRRGRQPPLLQLQEHHQAAPAAARPARAVARVLALRLHLLVLLVQQQQRQQQQQQQQRRRRRRRPDVRPELEFLPKRAQRQRAAAGGRRGGREPVPVSGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J520</accession>
    <name>A0A9L0J520_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Oligodendrocyte transcription factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">OLIG2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J520"/>
    <dbReference type="Ensembl" id="ENSEAST00005065896.1">
        <property type="protein sequence ID" value="ENSEASP00005048386.1"/>
        <property type="gene ID" value="ENSEASG00005029582.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161651"/>
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        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070888">
        <property type="term" value="F:E-box binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:HMG box domain binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042552">
        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045665">
        <property type="term" value="P:negative regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048663">
        <property type="term" value="P:neuron fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048714">
        <property type="term" value="P:positive regulation of oligodendrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007423">
        <property type="term" value="P:sensory organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021522">
        <property type="term" value="P:spinal cord motor neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021530">
        <property type="term" value="P:spinal cord oligodendrocyte cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021794">
        <property type="term" value="P:thalamus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd18940">
        <property type="entry name" value="bHLH_TS_OLIG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000031">
        <property type="entry name" value="Oligodendrocyte transcription factor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050359">
        <property type="entry name" value="bHLH_transcription_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032658">
        <property type="entry name" value="Olig2_bHLH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19290:SF32">
        <property type="entry name" value="OLIGODENDROCYTE TRANSCRIPTION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="108"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005048386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="322" mass="32288" checksum="5A90DD1725F1DBC6" modified="2023-09-13" version="1">MDSDASLVSSRPSSPEPDDLFLPARSKGSGGSGFTGGTVSSSTPSDCPPELSAELRGAMGTAGAHPGDKLGGGGFKSSSSSTSSSTSSAAASSTKKDKKQMTEPELQQLRLKINSRERKRMHDLNIAMDGLREVMPYAHGPSVRKLSKIATLLLARNYILMLTNSLEEMKRLVSEIYGGHHAGFHPSACGGLAHSAPLPAATAHPAAAAHAAHHPAVHHPILPPAAAAAAAAAAAAAVSSASLPGSGLSSVGSIRPPHGLLKSPSAAAAAPLGGGGGGSGGSGGFQHWGGMPCPCSMCQVPPPHHHVSAMGTGKPAAPHLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J6I9</accession>
    <name>A0A9L0J6I9_EQUAS</name>
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        <recommendedName>
            <fullName evidence="11">Centrosomal protein CEP57L1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Centrosomal protein 57kDa-like protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Centrosomal protein cep57l1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Centrosomal protein of 57 kDa-related protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Cep57-related protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CEP57L1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Centrosomal protein which may be required for microtubule attachment to centrosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the translokin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J6I9"/>
    <dbReference type="Ensembl" id="ENSEAST00005066704.1">
        <property type="protein sequence ID" value="ENSEASP00005044860.1"/>
        <property type="gene ID" value="ENSEASG00005006465.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063695"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.90:FF:000005">
        <property type="entry name" value="centrosomal protein CEP57L1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051756">
        <property type="entry name" value="Centrosomal_MT-associated"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025913">
        <property type="entry name" value="Cep57_CLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024957">
        <property type="entry name" value="Cep57_MT-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19336:SF10">
        <property type="entry name" value="CENTROSOMAL PROTEIN CEP57L1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR19336">
        <property type="entry name" value="UNCHARACTERIZED DUF1167"/>
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        <property type="entry name" value="Cep57_MT_bd"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
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            <begin position="55"/>
            <end position="232"/>
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        <location>
            <begin position="190"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="15">
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            <begin position="239"/>
            <end position="255"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="15">
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            <begin position="410"/>
            <end position="438"/>
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    </feature>
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    </evidence>
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    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="476" mass="55186" checksum="CD2FF301A8ECED08" modified="2023-09-13" version="1">MDPELMHSIVGSYLKTPERVLVPSFTQNDQSAPTHHSVSLEVNLPKMLHSPNSQALILALKTLQEKIHRLDLERTQAEDNLNVLSREAAQYKKALENETNERNLAHQELIKQKKDITIQLSSAQSRCTLLEKQLEYTKRMVLNVEREKNMILEQQAQLQREKEQDHLKLQAKLEKLDVLEKECFKLTTTQKTAEDKIKYLEEKLKEEEHHRKLFQDKASELQTGLEINRILMSSVSNPKRSKEKKKSSKKTKLKRGPPQQIYSLFGSLPIVVEKSASASHSVKASRQNLLQTMQHYGPHILPKRAEVTEPRRLYKPTRSTSQRKAVPPDSGKSICICNNVSELLMAMQDELDQMSMKHQELLNQIKETESHSVFENAECELEHLVKKMEIKVEQISKLKKHQDNVRKLQQKVQNSKMSEASGIQQEDNYPNGSNNLKNSPRKCLLNNPLQKSSNFHPIRVRNLQMKLRRDDIMWEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J7B9</accession>
    <name>A0A9L0J7B9_EQUAS</name>
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        <submittedName>
            <fullName evidence="7">Peptidoglycan recognition protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PGLYRP2</name>
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    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.</text>
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        <property type="gene ID" value="ENSEASG00005026655.1"/>
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        <property type="component" value="Chromosome 10"/>
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        <property type="term" value="F:N-acetylmuramoyl-L-alanine amidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0051701">
        <property type="term" value="P:biological process involved in interaction with host"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:detection of bacterium"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:negative regulation of natural killer cell differentiation involved in immune response"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:negative regulation of type II interferon production"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:regulation of inflammatory response"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="PGRP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peptidoglycan recognition protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peptidoglycan recognition protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036505">
        <property type="entry name" value="Amidase/PGRP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002502">
        <property type="entry name" value="Amidase_domain"/>
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    <dbReference type="InterPro" id="IPR015510">
        <property type="entry name" value="PGRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006619">
        <property type="entry name" value="PGRP_domain_met/bac"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022:SF66">
        <property type="entry name" value="N-ACETYLMURAMOYL-L-ALANINE AMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11022">
        <property type="entry name" value="PEPTIDOGLYCAN RECOGNITION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Amidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ami_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="N-acetylmuramoyl-L-alanine amidase-like"/>
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    </dbReference>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040212111" evidence="4">
        <location>
            <begin position="23"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidoglycan recognition protein family" evidence="6">
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            <begin position="394"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetylmuramoyl-L-alanine amidase" evidence="5">
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            <begin position="407"/>
            <end position="546"/>
        </location>
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            <begin position="145"/>
            <end position="179"/>
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    </feature>
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    </feature>
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        <location>
            <begin position="567"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="587" mass="62554" checksum="9BCB88DDAF09EC55" modified="2023-09-13" version="1">MAGQGVLWVLLGLLLRLEPGTATLPLVMDSVIQALAELEQKAPATEASHTASAWLLSAQDSGPHDALHHFLLEGRRLKATELDPPPLSPELRGLAKEVARHGVRDGQEHGVVLAPDGSTVAVQPLLAGLEAGLQGRRAVSLPLESTATPPDARAPSPAAGGSVPDVRVPSPGCRDASADVTSTDARAVFPNARAADLGITATSPDVRPGSPDVPASSPGAKAESPTTVDSLLVVTLAKDLGLTFLQGPQTLSHPGLGSEGCWDQLSAPRAFTLLDPQASPLTTAFLNGALDGALLGDYLSRASEPRPPLSRLLSQYYGAGVAGDPGFRSNFRRQNGGALISVPTLTQQVWGALILLQRLEPAQAPQLQGLSPEQLVQVATHASTEFMEAFLGCPAIHPRCRWGAAPYQGRPTPLQLPLGFLYVHHTYVPAPPCTAFEHCARNMRSMQRFHQDTRGWGDIGYSFVVGSDGYVYEGRGWHWVGAHTRGHNSRGFGVAFVGNYTAELPTETALRTVRDVLPRCAVRAGLLRPSYRLLGHRQLVPTTCPGDALFDLLRTWPRFDANVKPRTARRASRRGPPAMILPATDLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J857</accession>
    <name>A0A9L0J857_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Coiled-coil domain containing 188</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CCDC188</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
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                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
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                <person name="D.D."/>
                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="Ig_sub"/>
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        <property type="entry name" value="Ig_V-set"/>
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        <property type="entry name" value="TCR_variable_region"/>
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        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 16"/>
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    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
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        <property type="entry name" value="V-set"/>
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        <property type="entry name" value="IG"/>
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        <property type="entry name" value="IGv"/>
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        <property type="entry name" value="IG_LIKE"/>
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    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
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    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
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        <location>
            <begin position="3"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
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    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005046025.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="120" mass="13472" checksum="59C42942F961ADF1" modified="2023-09-13" version="1">MKPILISVFVMIFPHRGARAQTVTQPEDRISVTEGAPVQVNCNYSYSGSPYLFWYVQYPKRGLQLLLKHTSGESIKGFTANLNNDEGSFHLKKPSAQEEDSAMYYCALSDTVTGFTREAE</sequence>
</entry>
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    <accession>A0A9L0JAK9</accession>
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        <recommendedName>
            <fullName evidence="5">KRAB domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JAK9"/>
    <dbReference type="Ensembl" id="ENSEAST00005049739.1">
        <property type="protein sequence ID" value="ENSEASP00005050209.1"/>
        <property type="gene ID" value="ENSEASG00005038441.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163313"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003655">
        <property type="entry name" value="aKRAB"/>
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    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
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        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF157">
        <property type="entry name" value="ZINC FINGER PROTEIN 525"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50806">
        <property type="entry name" value="KRAB_RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="10"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="1">
        <location>
            <begin position="13"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="62" mass="6779" checksum="932F1E54E3A5649C" modified="2023-09-13" version="1">MAASPSAQTRVSVTFDDVAVTFTQEEWGQLDLAQRTLYQEVMLENCGLLVSLGKVPIPPLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JHM7</accession>
    <name>A0A9L0JHM7_EQUAS</name>
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            <fullName evidence="15">E3 ubiquitin-protein ligase RNF135</fullName>
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        <alternativeName>
            <fullName evidence="17">RING finger protein 135</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">RING-type E3 ubiquitin transferase RNF135</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">RNF135</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Zhuang"/>
                <person name="G."/>
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                <person name="Y."/>
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                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
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                <person name="J."/>
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                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
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                <person name="G."/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 24">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer. Interacts (homodimer) with RIGI (double-stranded RNA-bound oligomeric form); involved in both RIGI ubiquitination, oligomerization into filaments associated with viral RNAs and the bridging of these filaments. Interacts with UBE2D3 and UBE2N; E2 ubiquitin ligases involved in RNF135-mediated ubiquitination of RIGI and activation of the RIG-I signaling pathway. Interacts with PCBP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Stress granule</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044601378.1">
        <property type="nucleotide sequence ID" value="XM_044745443.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JHM7"/>
    <dbReference type="Ensembl" id="ENSEAST00005056648.1">
        <property type="protein sequence ID" value="ENSEASP00005051853.1"/>
        <property type="gene ID" value="ENSEASG00005033664.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106844544"/>
    <dbReference type="KEGG" id="eai:106844544"/>
    <dbReference type="CTD" id="84282"/>
    <dbReference type="GeneTree" id="ENSGT00830000128449"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043021">
        <property type="term" value="F:ribonucleoprotein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140374">
        <property type="term" value="P:antiviral innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039529">
        <property type="term" value="P:RIG-I signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd12902">
        <property type="entry name" value="SPRY_PRY_RNF135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000059">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000545">
        <property type="entry name" value="E3 ubiquitin-protein ligase RNF135"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
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    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
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    <dbReference type="InterPro" id="IPR003879">
        <property type="entry name" value="Butyrophylin_SPRY"/>
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    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
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        <property type="entry name" value="E3_ubiq-ligase_TRIM/RNF"/>
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    <dbReference type="InterPro" id="IPR006574">
        <property type="entry name" value="PRY"/>
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        <property type="entry name" value="RNF135_SPRY_PRY_dom"/>
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        <property type="entry name" value="SPRY_dom"/>
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    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
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    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
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    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
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    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
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    <dbReference type="PANTHER" id="PTHR25465">
        <property type="entry name" value="B-BOX DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25465:SF41">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
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    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00589">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0391">Immunity</keyword>
    <keyword evidence="6" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="20">
        <location>
            <begin position="21"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="B30.2/SPRY" evidence="21">
        <location>
            <begin position="236"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="92"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="19">
        <location>
            <begin position="100"/>
            <end position="120"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
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    <evidence type="ECO:0000256" key="5">
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            <dbReference type="ARBA" id="ARBA00022490"/>
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    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
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            <dbReference type="ARBA" id="ARBA00022679"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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            <dbReference type="ARBA" id="ARBA00022786"/>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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            <dbReference type="ARBA" id="ARBA00022859"/>
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    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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            <dbReference type="ARBA" id="ARBA00063475"/>
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            <dbReference type="ARBA" id="ARBA00080069"/>
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    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080456"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
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            <dbReference type="PROSITE" id="PS50188"/>
        </source>
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    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005051853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="427" mass="47337" checksum="C93D742F9365C849" modified="2023-09-13" version="1">MAARDTGTAIPVWLAEDDLGCVICHELLTWPVTLPCGHSFCRHCLTRALGEGRERRHWSCPTCRESAAQQPQLRKNTLLQDLADKYREAARELEAGPGRTPSPAPRPAPGPPRRAAPPPAAAKKSITEVGPELVDLVEQLIDIVKNLQSQRHLPESGRDNELSSLGTAFSGGMDLSLASPKVVTSNTPERKVSDILHDLEEILGKLRENFTWKEALDSQTRVEVLEAPSSSSCPLPDQSRPTPKRASRFAQWAISPTFDLRSLSCSLGVSQDCRTVTVSHDSQSYPWSHERFATCQVLCSQAFSSGHQYWEVDTQQCSHWAVGVASWGMSRDHILGRTKDSWCVEWKGTSQLSVWHMVKETVLGSDRPKVVGIWLDLEEGKLAFYSVATQEQLLYECSISASSPLHPAFWLYGLHPGNSLTIRQVKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JHX3</accession>
    <name>A0A9L0JHX3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Collectin-12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Collectin placenta protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">COLEC12</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="15">The extracellular domain forms a stable trimer. The extracellular domain interacts with fibrillar amyloid-beta peptide.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044629497.1">
        <property type="nucleotide sequence ID" value="XM_044773562.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JHX3"/>
    <dbReference type="Ensembl" id="ENSEAST00005052896.1">
        <property type="protein sequence ID" value="ENSEASP00005052799.1"/>
        <property type="gene ID" value="ENSEASG00005002701.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106845108"/>
    <dbReference type="CTD" id="81035"/>
    <dbReference type="GeneTree" id="ENSGT00950000183074"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03590">
        <property type="entry name" value="CLECT_DC-SIGN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000017">
        <property type="entry name" value="collectin-12 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050111">
        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033989">
        <property type="entry name" value="CD209-like_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058762">
        <property type="entry name" value="COLEC12_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26004">
        <property type="entry name" value="COLEC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="10 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-0176">Collagen</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0430">Lectin</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="38"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="20">
        <location>
            <begin position="614"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="439"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="220"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="505"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="516"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="534"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="571"/>
            <end position="585"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00064704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00081384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="742" mass="81598" checksum="85581DF926797194" modified="2023-09-13" version="1">MKDDFAEEDEVQSFGYKRFDIQEGTQCTKCKNNWALKFSIILLYILCALLTITVAILGYKVVEKMDNVTGGMETSRQTYDDKLTAVESDLKKLGDQTGKKAISTNSELSTFRSDILDLRQQLREITEKTSRNKDTLEKLQASGDVLVDRQSQLKETLENNTFLITTVNKTLQAYNGYVTNLQQDTSVLQGNLQSQMYSHNVVIMNLNNLNLTQVQQRNLITNLQRSVDDTSQAIQRIKNDFQNLQQVFLQAKKDTDWLKEKVQSLQTLAANNSALAKANNDTLEDMNSQLSSFTGQMDNITTASQANEQNLKDLQDVHRDAENRMAIKFSQLEERFQLFETDIVNIISNISYTAHHLRTLTSNLNEVRTTCTDTLTKHTDDLTSLNNTLANIRLDSVSLRMQQDMMRSRLDTEVANLSVIMEEMKLVDSKHGQLIKNFTILQGPPGPRGPKGDRGQQGPPGPTGNKGQKGEKGEPGPPGPAGERGPIGPVGPPGERGGKGSKGLQGPKGSRGSPGKPGPQGPNGDPGPPGPPGKDGLPGPQGPPGFQGLQGTVGEPGVPGPRGLPGLPGVPGMPGPKGPPGPPGPSGAVVPQALQNEPTAAPEANGCPPHWKNFTDKCYYFSVEKEIFEDAKLFCEDKSSHLVFINTREEQQWIKKQMVGRESHWIGLTDSEHENEWKWLDGTSPEYKNWKAGQPDNWGHGHGPGEDCAGLIYAGQWNDFQCEDINNFICEKDRETVLSSAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JJP5</accession>
    <name>A0A9L0JJP5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Neurofilament heavy polypeptide</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">200 kDa neurofilament protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Neurofilament triplet H protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">NEFH</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms heterodimers with NEFL; which can further hetero-oligomerize (in vitro). Forms heterodimers with INA (in vitro).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JJP5"/>
    <dbReference type="Ensembl" id="ENSEAST00005069491.1">
        <property type="protein sequence ID" value="ENSEASP00005053546.1"/>
        <property type="gene ID" value="ENSEASG00005010080.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161893"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097418">
        <property type="term" value="C:neurofibrillary tangle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005883">
        <property type="term" value="C:neurofilament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099160">
        <property type="term" value="C:postsynaptic intermediate filament cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098685">
        <property type="term" value="C:Schaffer collateral - CA1 synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099184">
        <property type="term" value="F:structural constituent of postsynaptic intermediate filament cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990830">
        <property type="term" value="P:cellular response to leukemia inhibitory factor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033693">
        <property type="term" value="P:neurofilament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060052">
        <property type="term" value="P:neurofilament cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048936">
        <property type="term" value="P:peripheral nervous system neuron axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099170">
        <property type="term" value="P:postsynaptic modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902513">
        <property type="term" value="P:regulation of organelle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000010">
        <property type="entry name" value="neurofilament heavy polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010790">
        <property type="entry name" value="DUF1388"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23214:SF1">
        <property type="entry name" value="NEUROFILAMENT HEAVY POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23214">
        <property type="entry name" value="NEUROFILAMENT TRIPLET H PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07142">
        <property type="entry name" value="DUF1388"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 15" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="IF rod" evidence="18">
        <location>
            <begin position="97"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="458"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="101"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="178"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="311"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="459"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="495"/>
            <end position="1074"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00046768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00076316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005053546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1074" mass="117749" checksum="CFB1CF0624D5C4D2" modified="2023-09-13" version="1">MMSFSGADALLGAPFAPLHGGGSLHYALARKGGAGGVRSAAGSSSGFHSWARTSVSSVSASPGRFRGAGAASSTDSLDTLSNGPEGCVVAAAAARSEKEQLQALNDRFAGYIDKVRQLEAHNRSLEGEAAALRQQQAGRAAMGELYEREVREMRGAVLRLGAARGQLRLEQEHLLEDIAHVRQRLDDEARQREEAEAAARALARFAQEAEAARVELQKKAQALQEECGYLRRHHQEEVGELLGQIQGSGAAQAQAQAEARDALKCDVTSALREIRAQLEGHAVQTTLQSEEWFRVRLDRLSEAAKVNTDAMRTAQEEITEYRRQLQARTTELEALKGTKDSLERQRSELEDRHQADIASYQEAIQQLDAELRNTKWEMAAQLREYQDLLNVKMALDIEIAAYRKLLEGEECRIGFGPSPFPLPEVLPKIPPISTHIKVKSEEKIKVVEKSEKETVILEEQTEEIQVTEEVTEEEEKEAKEEEEEAKSPSAGEAASPEKEEAKSPEKAKSPVKEEAKSPAEVKSPEKAKSPAEVKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPEKARSPSVKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPEKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPEKAKSPVKEEAKSPEKAKSPVKEEAKSPEKAKSPEKAKSPEKAKSPEKEEAKSPEKAKSPEKEEAKSPEKAKSPVKEEAKSPEKAKSPVKEEIKSPEKVKSPVKEEAKAPEKEVPKKEEAKSPKKEEEKAPATPKTEEKKDSKKDEVPKKEAPKPEVQEKKEPAVEKPKESKAEAKKEEAEDKKAVTPEKEAAAKVKEEAKPKEKTEVAKKEPEKPKKEETPAAPEKKDAKEEKVTEAKKPEEKPKTEVQAKTEKAEKSSSTDQKDSRPPEDKATKGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JKQ7</accession>
    <name>A0A9L0JKQ7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSEASP00005052953.1"/>
        <property type="gene ID" value="ENSEASG00005036997.1"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Ig_V-set"/>
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        <property type="entry name" value="TCR_variable_region"/>
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    <dbReference type="PANTHER" id="PTHR19367:SF24">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 8-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
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        <property type="entry name" value="IG_LIKE"/>
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    <keyword evidence="3" id="KW-0675">Receptor</keyword>
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    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040352418" description="Ig-like domain-containing protein" evidence="6">
        <location>
            <begin position="21"/>
            <end position="113"/>
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        <location>
            <begin position="20"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="113" mass="12677" checksum="D263C7EBA315134A" modified="2023-09-13" version="1">MASVTILLLGMLFTVRGTRAQSVTQPDDHITVSEGNSLELRCNFSYSGTPYLFWYVQYPSQGLQLLLKDITRDTLVEGIKGFEAEFNKSETSFHLRKPSAHFSDSAVYFCAVS</sequence>
</entry>
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    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="J."/>
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                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">May be involved in modulating the expression of photoreceptor specific genes. Binds to the Ret-1 and Bat-1 element within the rhodopsin promoter.</text>
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    <comment type="subunit">
        <text evidence="2">Interacts with CRX.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JLT9"/>
    <dbReference type="Ensembl" id="ENSEAST00005062115.1">
        <property type="protein sequence ID" value="ENSEASP00005054189.1"/>
        <property type="gene ID" value="ENSEASG00005026320.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163572"/>
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        <property type="component" value="Chromosome 20"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000071">
        <property type="entry name" value="Retinal homeobox gene 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
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    </dbReference>
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        <property type="entry name" value="HD"/>
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    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043562">
        <property type="entry name" value="RAX/RAX2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46271">
        <property type="entry name" value="HOMEOBOX PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46271:SF2">
        <property type="entry name" value="RETINA AND ANTERIOR NEURAL FOLD HOMEOBOX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="25"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="27"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011785"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00019389"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005054189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="184" mass="19994" checksum="13D1F2133BA3DECA" modified="2023-09-13" version="1">MFLSTGEGPAAEGGAPGPGEEAPKKQHRRNRTTFTTYQLHQLERAFEASHYPDVYSREELAAKVHLPEVRVQVWFQNRRAKWRRQERLESGSGAVAAPRLPEAPALPFARPPAVPLPLEPWLGPGPPAGPGLPRLLGPGPGLQATFAPHAFGPAFSDGFALEEASLRLLAKEHAQALDRAWPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JNS8</accession>
    <name>A0A9L0JNS8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Rho GTPase-activating protein 36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ARHGAP36</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JNS8"/>
    <dbReference type="Ensembl" id="ENSEAST00005071588.1">
        <property type="protein sequence ID" value="ENSEASP00005050820.1"/>
        <property type="gene ID" value="ENSEASG00005014377.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153904"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902533">
        <property type="term" value="P:positive regulation of intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04376">
        <property type="entry name" value="RhoGAP_ARHGAP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000017">
        <property type="entry name" value="Rho GTPase activating protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041852">
        <property type="entry name" value="ARHGAP6_RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037863">
        <property type="entry name" value="RHOGAP6/36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12635:SF8">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12635">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN 6 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="5">
        <location>
            <begin position="195"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="458"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="493"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00055252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00070267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="516" mass="58864" checksum="AB0119E078170351" modified="2023-09-13" version="1">MNVLGGAPGHNPDRRTKMISIHSLSELERLKLQETAYHELVARHFLSEFKPDRALPIDRPNTLEKWFLILRGQQRVISLKTFGIRLEEVLVNELTRRKHLELRAEMQIEESTGQALGRRRGNVVQRMFGRIRRFFSRRRDEPSLPREFTRRGRRGAVSVDSLAELEDGALLLQTLQLSKISFPIGQRLLGSKRKMSLNPIAKQIPQVVEVCCNFIEKHGLSTVGIFTLEYSEERVRELREEFDQGLDIVLDDTQNVHDVAALLKEFFRDMKDSLLPDDLYMSFLLTATLKPQDQLSALQLLVYLMPPCHSDTLERLLKALHKITENCEDSIGFDGQLVSGNRMTSTNLALVFGSVLLKKGRFAKRESRKTRLGIDHYVASVNVVRAMIDNWDILFQVPPHIQRQVAKRVWKSSPEALDFIRRRNLRKIQSARIKMEEDALLSDPVESSAEARAAVLAQSKPFDEGSSEEPAVPPGTARSHDDEEGAGNPPILEQDRPLLRVPREKEAKTGIGYFFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JPD2</accession>
    <name>A0A9L0JPD2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E3 ubiquitin-protein ligase pellino homolog</fullName>
            <ecNumber evidence="3">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PELI3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pellino family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0JPD2"/>
    <dbReference type="Ensembl" id="ENSEAST00005082595.1">
        <property type="protein sequence ID" value="ENSEASP00005051847.1"/>
        <property type="gene ID" value="ENSEASG00005030333.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183050"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001237">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010804">
        <property type="term" value="P:negative regulation of tumor necrosis factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070534">
        <property type="term" value="P:protein K63-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008592">
        <property type="term" value="P:regulation of Toll signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006800">
        <property type="entry name" value="Pellino_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048334">
        <property type="entry name" value="Pellino_FHA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048335">
        <property type="entry name" value="Pellino_RING"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12098:SF9">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PELLINO HOMOLOG 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12098">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PELLINO-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04710">
        <property type="entry name" value="Pellino_FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20723">
        <property type="entry name" value="Pellino_RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038886">
        <property type="entry name" value="Pellino"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Pellino FHA" evidence="5">
        <location>
            <begin position="59"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Pellino RING" evidence="6">
        <location>
            <begin position="271"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="36"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR038886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005051847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="414" mass="44925" checksum="4DF42D832904A1D8" modified="2023-09-13" version="1">MVLEGNPEVGSPRTSDLRHPGNLPFALLHRLPLSGVKQPSHQGWGVSIQHPPGQPDQGPQLSPALSNRGQHSISYTLSRSHSVIVEYTHDSDTDMFQIGRSTENMIDFVVTDTSPGGGAAEGPSAQSTISRYACRILCDRRPPYTARIYAAGFDASSNIFLGERAAKWRTPDGLMDGLTTNGVLVMHPAGGFSEDSAPGVWREISVCGNVYTLRDSRSAQQRGKLVENESNVLQDGSLIDLCGATLLWRTPAGLLRAPTLKQLEAQRQEANAARPQCPVGLSTLAFPSPARGRTAPDKQQPWVYVRCGHVHGYHGWGCRRERGPQERECPLCRLVGPYVPLWLGQEAGLCLDPGPPSHAFAPCGHVCSEKTARYWAQTPLPHGTHAFHAACPFCGAWLTGEHGCVRLIFQGPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JQI4</accession>
    <name>A0A9L0JQI4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Transmembrane protease serine 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Matriptase-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">TMPRSS6</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">Interacts with HJV.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JQI4"/>
    <dbReference type="Ensembl" id="ENSEAST00005038495.1">
        <property type="protein sequence ID" value="ENSEASP00005051415.1"/>
        <property type="gene ID" value="ENSEASG00005018878.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160104"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033619">
        <property type="term" value="P:membrane protein proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060586">
        <property type="term" value="P:multicellular organismal-level iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097264">
        <property type="term" value="P:self proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000027">
        <property type="entry name" value="Transmembrane serine protease 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.960:FF:000004">
        <property type="entry name" value="Transmembrane serine protease 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000096">
        <property type="entry name" value="Transmembrane serine protease 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000097">
        <property type="entry name" value="Transmembrane serine protease 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.960">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF20">
        <property type="entry name" value="LOW QUALITY PROTEIN: TRANSMEMBRANE PROTEASE SERINE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50024">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 18" id="KW-0645">Protease</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 18" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="SEA" evidence="21">
        <location>
            <begin position="36"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="20">
        <location>
            <begin position="287"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="22">
        <location>
            <begin position="514"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="360"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="428"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="447"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="468"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="488"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00071698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00077327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005051415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="748" mass="82685" checksum="C573F8EA581170AF" modified="2023-09-13" version="1">GPRCWRLSRGFGPPCLRPGLILPVSSWAGYRAEVTVSQVYSGSLRVLNRHFSQDLARRESSAFRSETAKAQKMLKGLIASTRLGTYYNSSSVYSFGEGPFTCFFWFILQIPEHRRPMLSPEVVRALLVEELLSTANSSAPPPYLAEYELDPEGLVLLEASVKDLVALNSTLGCYRYSYVGQGQVLRLKGPDQLASSCLWHLQGPQDLMLKLRLEWKLADCRDRLAMYDVAGPLERKLITSVYGCSRQEPVVEVLASGAVMAVVWKKGLHSYQDPFVLSVQPVAFQACEVNLTLEGRLEPQGALRTPHFPSYYSPSTHCSWHLTVPSLEYGLALWFDAYALRRQKYGLPCTQGQWTIQNRRYPPPGAPSSPHRCPHLEPNSLTSEGSTGRERSTSTSLWGAFNPCSGCVSLICKGIPFCGGTPFPVGVCRATFQCQEDSTCVSLSRVCDQQPDCLNGSDEEQCQEGVPCGTFTFQCEDRSCVKKPNPQCDGQPDCRDGSDEQHCDCGLQGPSGRIVGGAVSSEGEWPWQASLQVRGRHICGGALIADRWVITAAHCFQEDSMASPALWTVFLGKVWQSSRWPGEVSFKVSRLVLHPYHEEDSHDYDVALLQLDHPVVRSAAVHPVCLPARSHFFEPGLHCWITGWGALREGGPTSNGLQKVDVQLIPQDLCSEAYRYRVTPRMLCAGYHKGKKDACQGDSGGPLVCREPSGRWFLAGLVSWGLGCGRPNYFGVYTRITGVIGWIQQVLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JRX7</accession>
    <name>A0A9L0JRX7_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">S100 calcium binding protein A13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">S100A13</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JRX7"/>
    <dbReference type="Ensembl" id="ENSEAST00005081176.1">
        <property type="protein sequence ID" value="ENSEASP00005055949.1"/>
        <property type="gene ID" value="ENSEASG00005030025.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161854"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
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    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:extracellular space"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:nucleolus"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017134">
        <property type="term" value="F:fibroblast growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050786">
        <property type="term" value="F:RAGE receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032730">
        <property type="term" value="P:positive regulation of interleukin-1 alpha production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd05022">
        <property type="entry name" value="S-100A13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013787">
        <property type="entry name" value="S100_Ca-bd_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639:SF57">
        <property type="entry name" value="PROTEIN S100-A13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639">
        <property type="entry name" value="S100 CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01023">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S100/CaBP-9k-type calcium binding subdomain" evidence="1">
        <location>
            <begin position="49"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005055949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="123" mass="13892" checksum="98899CC2E1A892B3" modified="2023-09-13" version="1">PLHHRAGPCACVYWSSILTGLYLGEAEKLSPPLLVGTLNPYLPGLEHSSGREGRKGSLSVNEFKELATQQLPHLLKDVGSLDEKMKSLDVNQDSELKFHEYWRLIGELAKEIRKEKAQEIRKK</sequence>
</entry>
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    <accession>A0A9L0JU11</accession>
    <name>A0A9L0JU11_EQUAS</name>
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        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC106829779</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JU11"/>
    <dbReference type="Ensembl" id="ENSEAST00005044321.1">
        <property type="protein sequence ID" value="ENSEASP00005053502.1"/>
        <property type="gene ID" value="ENSEASG00005031309.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154646"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20679">
        <property type="entry name" value="CYP4F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000005">
        <property type="entry name" value="cytochrome P450 4F22 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF198">
        <property type="entry name" value="CYTOCHROME P450 4F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="475"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="531" mass="60578" checksum="6F72EDF2247F511B" modified="2023-09-13" version="1">MPRLSLSWLGLGPLAASPWLLLLLVGASWLLARVLAWTYTFYDNCCRLRCFPQPPKRNWFWSHLGLVTHTEQGMRVVTQLVANYPKGFVTWLGPVIPLVTLCHPDMVRTVLSASAAIAPKDMVFYSFLKPWLGDGLLLSGGDKWSRHRRMLTPAFHFNILKTYVRIFNDSVNIMHAKWKCLASEGSAHLDMFEHISLMTLDSLQKCVFSFNSDCQEKPSEYISGVLELSALVRKRDEQIFLYMDSLYHLTSEGQRFRRVCRLVHDFTDSIIQERRRTLPDQGLDDLLKAKAKVKTLDFIDVLLLAKDEDGKELSDEDIRAEVDTFIDPNLSGCLSGHDTTASGLSWVLYNLARHPEHQERCRQEVRELLRDREPKEIEWDNLAQMPFLTMCIKESLRLHPPAVGTSQRCTQDIVLPDGRVIPKGVTCVISIFGTHHNPSVWPDAEVYDPFRFDPENTKERSPLAFIPFSAGPRNCIGQTFAMTEMKVVLALTLLRFRVLPAGEEPRRKPELILRAEGGLWLRVEPLSVGPQ</sequence>
</entry>
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    <accession>A0A9L0JV98</accession>
    <name>A0A9L0JV98_EQUAS</name>
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            <fullName evidence="11">Ephrin-A3</fullName>
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        <alternativeName>
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        <alternativeName>
            <fullName evidence="12">EPH-related receptor tyrosine kinase ligand 3</fullName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with EPHA8; activates EPHA8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14 15">Belongs to the ephrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044614304.1">
        <property type="nucleotide sequence ID" value="XM_044758369.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JV98"/>
    <dbReference type="Ensembl" id="ENSEAST00005039410.1">
        <property type="protein sequence ID" value="ENSEASP00005057236.1"/>
        <property type="gene ID" value="ENSEASG00005033980.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000161355"/>
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        <property type="component" value="Chromosome 25"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:ephrin receptor binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016525">
        <property type="term" value="P:negative regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902993">
        <property type="term" value="P:positive regulation of amyloid precursor protein catabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd10425">
        <property type="entry name" value="Ephrin-A_Ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000030">
        <property type="entry name" value="ephrin-A3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031328">
        <property type="entry name" value="Ephrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034252">
        <property type="entry name" value="Ephrin-A_Ecto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019765">
        <property type="entry name" value="Ephrin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001799">
        <property type="entry name" value="Ephrin_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304">
        <property type="entry name" value="EPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304:SF5">
        <property type="entry name" value="EPHRIN-A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00812">
        <property type="entry name" value="Ephrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01347">
        <property type="entry name" value="EPHRIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01299">
        <property type="entry name" value="EPHRIN_RBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EPHRIN_RBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040105000" description="Ephrin-A3" evidence="16">
        <location>
            <begin position="23"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Ephrin RBD" evidence="17">
        <location>
            <begin position="30"/>
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        </location>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00078736"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00084021"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00884"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005057236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="238" mass="26335" checksum="DF86A03C6384ED00" modified="2023-09-13" version="1">MAAAPLLLLLLLVPVPLLPLLAQGPGGALGNRHAVYWNSSNQHLRREGYTVQVNVNDYLDIYCPHYNSSGVGPGAGPGPGGGAEQYVLYMVSRTGYRTCNASQGFKRWECNRPHAPHSPIKFSEKFQRYSAFSLGYEFHAGHEYYYISTPTHNLHWKCLRMKVFVCCASTSHSGEKPVPTLPQFTMGPNVKINVLEDFEGENPQVPKLEKSISGTSPKREHLPLAMGIAFFLMTLLAS</sequence>
</entry>
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    <accession>A0A9L0JVN3</accession>
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        <submittedName>
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        </submittedName>
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        <name evidence="2" type="primary">TMEM89</name>
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        <name type="scientific">Equus asinus</name>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
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                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
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                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_014695828.1">
        <property type="nucleotide sequence ID" value="XM_014840342.3"/>
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        <property type="gene ID" value="ENSEASG00005036657.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="eai:106830665"/>
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    <dbReference type="OrthoDB" id="9833607at2759"/>
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        <location>
            <begin position="41"/>
            <end position="64"/>
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        <location>
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            <end position="134"/>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="207" mass="22852" checksum="041A5579D11780DA" modified="2023-09-13" version="1">MPHVFCSGFPHERPWGQLEPPASSLACLWGRGVCLLPGAQVPLALATAMLLARSFLLVLLLLAMPAPTHTWSRPLWYQVGLDLQPWGCQPDSMESCRESLGCPGHLMGLGENYVYPVAGVTATTTMMLMIAHVVQRRWRLRATKFEHPQVTTDPPGLWKQRTPISDRTLLLGVLHVLDALLVHIEGHLQSLATQQRTQIKGTPTQSG</sequence>
</entry>
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                <person name="Li"/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Yin"/>
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                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFB1 subunit family.</text>
    </comment>
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        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 1"/>
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    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
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</entry>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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    </evidence>
    <sequence length="151" mass="16563" checksum="09432A08B5A0E6A2" modified="2023-09-13" version="1">WGARVFKLPYGTGKGLILQKLNVERTQRIIARSQKIQAKEEGNAVPLCQAKPSPSFINLQASSPPATLLNIQTTKLRSRVNQKSKECLGLLECMYANLQLQTQLAQQQMAILENLQASMTQLATGGGESKNSSLPALAHNLLLNHLPQFSK</sequence>
</entry>
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    <accession>A0A9L0K2U1</accession>
    <name>A0A9L0K2U1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lysine-rich coiled-coil protein 1</fullName>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K2U1"/>
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        <property type="entry name" value="LYSINE RICH COILED-COIL 1"/>
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        <location>
            <begin position="105"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005055660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="23040" checksum="B598E7497E39B6CA" modified="2023-09-13" version="1">MENSMAYDSFQHELEDYIRKQKARGLQPEHYFRKVTEDSGYRERGHPAPRPLRLEQSLFRSSHRFPGSYQRLRTVESQLRPRSKIHHSRQILESLNHCQKNHDNFLRNPWLPRPGGEAQVERKRKHRADGDSHKENRGRAQAEPGSAEKPKYSKKSNQDPVTTKEGRRSGREKKRSAKESPRETDLWDEAILGSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K356</accession>
    <name>A0A9L0K356_EQUAS</name>
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        <recommendedName>
            <fullName evidence="9 11">Nucleoside diphosphate-linked moiety X motif 6</fullName>
            <shortName evidence="11">Nudix motif 6</shortName>
            <ecNumber evidence="11">3.6.1.-</ecNumber>
        </recommendedName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
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                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 11">May contribute to the regulation of cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 11">Monomer and homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the Nudix hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="11"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:ADP-ribose diphosphatase activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0047884">
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:NADH pyrophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045786">
        <property type="term" value="P:negative regulation of cell cycle"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd04670">
        <property type="entry name" value="NUDIX_ASFGF2_Nudt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000062">
        <property type="entry name" value="Nucleoside diphosphate-linked moiety X motif 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000027">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.100:FF:000001">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.100">
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    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR003293">
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        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE-LINKED MOIETY X MOTIF 6"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NUDIXFAMILY"/>
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        <property type="entry name" value="NUDIX_BOX"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="12">
        <location>
            <begin position="138"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00057091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368106"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005055780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="313" mass="34947" checksum="82B8F85AC26CBFBE" modified="2023-09-13" version="1">MPPLPSGVCWRTVLARAHRARLSSGSRGASGDAGCAQDHPAVVGELRGELDRFGGVSVRLGALDRLDAAAFQRGLQAAIQQWRSEGRIAVWLHIPILQSRFIAPAASLGFCFHHAESDSSTLTLWLGEGPSRLPGYATHQVGVAGAVFDENTRKILVVQDRNKLKNMWKFPGGLSEPGEDIGDTAVREVFEETGIKSEFRSLLSIRQQHTNPGAFGKSDMYIICRLQPCSFTINFCQHECLRCEWMDLNDLVKTENTTPITSRVARLLLYGYREGFDKIDLTMEELPAVYTGLFYKLYHRKLPDKYKTMTLMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K450</accession>
    <name>A0A9L0K450_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Xylosyltransferase 2</fullName>
            <ecNumber evidence="9">2.4.2.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Xylosyltransferase II</fullName>
        </alternativeName>
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    <gene>
        <name evidence="29" type="primary">XYLT2</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
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        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="25">Catalyzes the first step in the biosynthesis of chondroitin sulfate, heparan sulfate and dermatan sulfate proteoglycans, such as DCN. Transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>UDP-alpha-D-xylose + L-seryl-[protein] = 3-O-(beta-D-xylosyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50192"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12567"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132085"/>
            <dbReference type="EC" id="2.4.2.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Glycan metabolism; chondroitin sulfate biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Glycan metabolism; heparan sulfate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Golgi apparatus membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the glycosyltransferase 14 family. XylT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.26" evidence="9"/>
    <dbReference type="RefSeq" id="XP_014689238.1">
        <property type="nucleotide sequence ID" value="XM_014833752.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K450"/>
    <dbReference type="Ensembl" id="ENSEAST00005040611.1">
        <property type="protein sequence ID" value="ENSEASP00005057122.1"/>
        <property type="gene ID" value="ENSEASG00005018617.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106826421"/>
    <dbReference type="KEGG" id="eai:106826421"/>
    <dbReference type="CTD" id="64132"/>
    <dbReference type="GeneTree" id="ENSGT00940000158326"/>
    <dbReference type="OMA" id="WENAHDI"/>
    <dbReference type="OrthoDB" id="2019572at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:manganese ion binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030158">
        <property type="term" value="F:protein xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050650">
        <property type="term" value="P:chondroitin sulfate proteoglycan biosynthetic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:glycosaminoglycan biosynthetic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030210">
        <property type="term" value="P:heparin proteoglycan biosynthetic process"/>
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    </dbReference>
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        <property type="entry name" value="Glyco_trans_14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043538">
        <property type="entry name" value="XYLT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024448">
        <property type="entry name" value="XylT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46025:SF1">
        <property type="entry name" value="XYLOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46025">
        <property type="entry name" value="XYLOSYLTRANSFERASE OXT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02485">
        <property type="entry name" value="Branch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12529">
        <property type="entry name" value="Xylo_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="22" id="KW-0464">Manganese</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0964">Secreted</keyword>
    <keyword evidence="16" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Xylosyltransferase C-terminal" evidence="28">
        <location>
            <begin position="519"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="39"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="27">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00010195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00011972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF12529"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005057122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="865" mass="96849" checksum="E0848989B6E50042" modified="2023-09-13" version="1">MVASARVQKLVRRYKLAIATALAILLLQGLVVWSFSGLEEDEPGEKGRQRKPRPLDPGEGSKDTDSSAGRRGSAGRRHGRWRGRAESPGVPVAKVVRAVTSRHRASRRVPPAPPPEAPGRQNLSGAAAGEALVGAAGFPPHGDTGSVEGAPQPTDNGFTPKCEIVGKDALSALARASSKQCQQEIANVVCLHQAGNLMPKAVPRHCQLAGKMSPGIQWDEVQAQQPVDGPPVRIAYMLVVHGRAIRQLKRLLKAVYHKQHFFYIHVDKRSNYLHREVVELARQYDNVRVTPWRMVTIWGGASLLRMYLRSMQDLLEVPGWAWDFFINLSATDYPTRTNEELVAFLSKNRDKNFLKSHGRDNSRFIKKQGLDRLFHECDSHMWRLGERQIPAGIVVDGGSDWFVLTRSFVEYVVYTDDPLVAQLRQFYTYTLLPAESFFHTVLENSPACESLVDNNLRVTNWNRKLGCKCQYKHIVDWCGCSPNDFKPQDFLRLQQVSRPTFFARKFESTVNQEVLEILDFHLYGSYPPGTPALKAYWENTYDMADGPSGLSDVMLTAYTAFARLSLRHAATAASPLATPLCRFEPRGLPSSVHLYFYDDHFQGYLVTQAVQPSAQGPAETLEMWLMPQGSLKLLGHSDQASRLQSLEVGTEWDPKERIFRNFGGLLGPLDEPMAMQRWARGPNLTATVVWIDPTYVVATSYDIAVDTETEVTQYKPPLSRPLRPGAWTVRLLQFWEPLGEIRFLVLPLTFNRKLPLRKDDASWLHAGPPHNEYMEQSFQGLSGILNLPQPELVEEAARRHVELTGPALEAWTDGELSSFWSVAGLCAMGPSACPSLELCRLTSWSSLFPDPKSELGPVKADGRLR</sequence>
</entry>
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    <name>A0A9L0K5R7_EQUAS</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="8" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_V-set"/>
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        <property type="entry name" value="TCR_variable_region"/>
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    <dbReference type="PANTHER" id="PTHR19367:SF24">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 8-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040460447" description="Ig-like domain-containing protein" evidence="6">
        <location>
            <begin position="21"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="21"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005057712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="117" mass="12907" checksum="0F04760AFF68379A" modified="2023-09-13" version="1">MFSVTILLLGMLLTVRGTGAQSVTQPNDHISVSEETSLELKCNYSSGNLYFFWYVQYPSQGLQLLLRYSSGPTLVNGIKGFEAELKKNETSFHLRKSSAHLGDSAVYFCAASDTVTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K7I1</accession>
    <name>A0A9L0K7I1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">UPAR/Ly6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123290289</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K7I1"/>
    <dbReference type="Ensembl" id="ENSEAST00005050034.1">
        <property type="protein sequence ID" value="ENSEASP00005057285.1"/>
        <property type="gene ID" value="ENSEASG00005027834.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154560"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033130">
        <property type="term" value="F:acetylcholine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030550">
        <property type="term" value="F:acetylcholine receptor inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0095500">
        <property type="term" value="P:acetylcholine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018363">
        <property type="entry name" value="CD59_antigen_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016054">
        <property type="entry name" value="LY6_UPA_recep-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051445">
        <property type="entry name" value="LY6H/LY6L_nAChR_modulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32217">
        <property type="entry name" value="LYMPHOCYTE ANTIGEN 6H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32217:SF5">
        <property type="entry name" value="LYMPHOCYTE ANTIGEN 6H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00021">
        <property type="entry name" value="UPAR_LY6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00983">
        <property type="entry name" value="LY6_UPAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040322358" description="UPAR/Ly6 domain-containing protein" evidence="9">
        <location>
            <begin position="21"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="10">
        <location>
            <begin position="20"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005057285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="139" mass="14603" checksum="E3C7C63CB4B2B15C" modified="2023-09-13" version="1">MKGIHLLLLAVLLCSQQALSLRCYSCADVENINKCLLIFSCAPTPSVCYKGSMTLTTATGETATINPKGCAPSCQEASQVMQLIANLSNPTGAFNLEVRDVACCEKDLCNGVAQVARSLWALAGGLLLSLGPALLWALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K8P3</accession>
    <name>A0A9L0K8P3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrator complex subunit 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">von Willebrand factor A domain-containing protein 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">INTS14</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Component of the Integrator complex, composed of core subunits INTS1, INTS2, INTS3, INTS4, INTS5, INTS6, INTS7, INTS8, INTS9/RC74, INTS10, INTS11/CPSF3L, INTS12, INTS13, INTS14 and INTS15. The core complex associates with protein phosphatase 2A subunits PPP2CA and PPP2R1A, to form the Integrator-PP2A (INTAC) complex. INTS14 is part of the tail subcomplex, composed of INTS10, INTS13, INTS14 and INTS15.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the Integrator subunit 14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014711655.1">
        <property type="nucleotide sequence ID" value="XM_014856169.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014711658.1">
        <property type="nucleotide sequence ID" value="XM_014856172.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044620623.1">
        <property type="nucleotide sequence ID" value="XM_044764688.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044620624.1">
        <property type="nucleotide sequence ID" value="XM_044764689.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044620625.1">
        <property type="nucleotide sequence ID" value="XM_044764690.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K8P3"/>
    <dbReference type="Ensembl" id="ENSEAST00005044799.1">
        <property type="protein sequence ID" value="ENSEASP00005061719.1"/>
        <property type="gene ID" value="ENSEASG00005006454.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106840594"/>
    <dbReference type="KEGG" id="eai:106840594"/>
    <dbReference type="CTD" id="81556"/>
    <dbReference type="GeneTree" id="ENSGT00390000009486"/>
    <dbReference type="OMA" id="QSSVVWI"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032039">
        <property type="term" value="C:integrator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034472">
        <property type="term" value="P:snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000137">
        <property type="entry name" value="Integrator complex subunit 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039841">
        <property type="entry name" value="INTS14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045814">
        <property type="entry name" value="IntS14_b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046471">
        <property type="entry name" value="IntS14_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13532">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13532:SF3">
        <property type="entry name" value="INTEGRATOR COMPLEX SUBUNIT 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19435">
        <property type="entry name" value="IntS14_b-barrel"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="IntS14_C"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="3"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 14 beta-barrel" evidence="10">
        <location>
            <begin position="209"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Integrator complex subunit 14 C-terminal" evidence="11">
        <location>
            <begin position="404"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016816"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029992"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061449"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065091"/>
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        <source>
            <dbReference type="Pfam" id="PF19435"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="518" mass="57442" checksum="76B29A94220EF083" modified="2023-09-13" version="1">MPTVVVMDVSLSMTRPVSLEGSEEYQRKHLAAHGLTMLFEHMATNYKLEFTALVVFSSLWELMVPFTRDYNTLQEALSNMDDYDKTCLESALVGVCNIVQQEWGGAIPCQVVLVTDGCLGIGRGSLRHSLATHSQRSENNRFPLPFPFPSKLYIMCMANLEELQSTDSLDCLERLIDLNNGEGQIFTIDGPLCLKNVQSMFGKLIDLAYTPFHAVLKCGHLTADVQVFPRPEPFVVDEEIDPVPKVINTDLEIVGFIDIADISSPPVLSRHLVLPIALNKEGDEVGTGITDDNEDENSANQIAGKIPNFCVLLHGSLKVEGMVAIVQLGPEWHGMLYSQADSKKKSNLMMSLFEPGPEPLPWLGKMAQLGPISDAKENPYGEDDNKSPFPLQPKNKRSYAQNVTVWIKPSGLQTDVQKILRNARKLPEKTQTFYKELNRLRKAALAFGFLDLLKGVADMLERECTLLPDTAHPDAAFQLTHAAQQLKLASTGTSEYAGYDHNITPLQTDFSGSSTERM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K931</accession>
    <name>A0A9L0K931_EQUAS</name>
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        <submittedName>
            <fullName evidence="16">Zinc finger protein 792</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ZNF792</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K931"/>
    <dbReference type="Ensembl" id="ENSEAST00005039240.1">
        <property type="protein sequence ID" value="ENSEASP00005061343.1"/>
        <property type="gene ID" value="ENSEASG00005021397.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162928"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002682">
        <property type="term" value="P:regulation of immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001158">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001882">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001174">
        <property type="entry name" value="zinc finger protein 527 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000281">
        <property type="entry name" value="Zinc finger protein 558 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000098">
        <property type="entry name" value="Zinc finger protein 614"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF68">
        <property type="entry name" value="ZINC FINGER PROTEIN 358-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040390813" evidence="14">
        <location>
            <begin position="29"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="302"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="330"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="358"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="386"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="414"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="442"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="470"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="498"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="526"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="549"/>
            <end position="581"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="601" mass="68255" checksum="A4C3E43977ED98F5" modified="2023-09-13" version="1">MWFLLAMTTGMFLPGLPWLTSFRSHVVAQLELGAEPWVPDRVDMTSAMARGLYGRLGSDFCCGTKCEGSPSEHSVSVERVSQNGSPKAALSAQKDYPCDTCGQHLKDIPHLAEHQATHPRQKPYVCEAYGRECRSKTNLPQQQMRENVDKPIRREEGRASLVKSCTDDTSKKPLTFREGGEDFVARSGFLQHQVTHCVEEPQQNTEGVGDFHTVQSHGKCSEFGNTFSNKPTLVQPQRTPAGERPYECSKCGIFFSHAAGLSQHQRDHNRGKPYECCECGKFFSQHSSLVKHQRVHTGESPHVCGECGKFFSRSSNLIQHKRVHTGERPYECSECGKFFSQRSNLIHHKRVHTGKSAHECSECGKSFNCNSSLIKHWRVHTGERPYKCNECGKFFSHIASLIQHQIVHTGERPFGCSECGKAFSRSSDLMKHRRVHTGERPYECTECGKLFSQSSSLNSHRRLHTGERPYRCPECGKFFNQSSSLNNHRRLHTGERPYECLECGKTFRQRSNLRQHQKVHKPDRPYTCNECGKAFSQRPTLIRHQKIHTRERSAEKVLPPPLTRRHTLERSSESSPYEGALSQKLSLVHPNIHAGEVPYQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KA21</accession>
    <name>A0A9L0KA21_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Small leucine rich protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SMLR1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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        <scope>IDENTIFICATION</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <property type="term" value="P:branching involved in ureteric bud morphogenesis"/>
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    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
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        <property type="term" value="P:cell proliferation in midbrain"/>
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        <property type="term" value="P:central nervous system morphogenesis"/>
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        <property type="term" value="P:cerebellum formation"/>
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        <property type="term" value="P:diencephalon development"/>
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        <property type="term" value="P:embryonic axis specification"/>
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        <property type="term" value="P:forebrain anterior/posterior pattern specification"/>
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        <property type="term" value="P:midbrain dopaminergic neuron differentiation"/>
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        <property type="term" value="P:positive regulation of hematopoietic stem cell proliferation"/>
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        <property type="entry name" value="PROTO-ONCOGENE WNT-1"/>
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    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
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    </evidence>
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    <sequence length="363" mass="40147" checksum="7CF5A80356460837" modified="2023-09-13" version="2">MGHWALLPGWVSATLLLALAALPAALAANSSGRWWGIVNVASSTNLLTDSKSLQLVLEPSLQLLSRKQRRLIRQNPGILHSVSGGLQSAVRECKWQFRNRRWNCPTASGPHLFGKIVNRGCRETAFIFAITSAGVTHSVARSCSEGSIESCTCDYRRRGPGGPDWHWGGCSDNIDFGRLFGREFVDSGEKGRDLRFLMNLHNNEAGRTTVFSEMRQECKCHGMSGSCTVRTCWMRLPTLRAVGDVLRDRFDGASRVLYGNRGSNRASRAELLRLEPEDPAHKPPSPHDLVYFEKSPNFCTYSGRLGTAGTAGRACNSSSPALDGCELLCCGRGHRTRTQRVTERCTFTERCNCTPLRVLRECL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KT73</accession>
    <name>A0A8C4KT73_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106830115</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KT73"/>
    <dbReference type="Ensembl" id="ENSEAST00005001375.2">
        <property type="protein sequence ID" value="ENSEASP00005001215.2"/>
        <property type="gene ID" value="ENSEASG00005001018.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286912"/>
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        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15952">
        <property type="entry name" value="7tmA_OR52E-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000006">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF77">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="115"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="219"/>
            <end position="243"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="263"/>
            <end position="282"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="294"/>
            <end position="316"/>
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    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="64"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022606"/>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363047"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
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    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001215.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="332" mass="37114" checksum="F7A9135803544356" modified="2023-09-13" version="2">MSKKEKQDSHIQMIFFNKIGKMFHTNTSTFHPDTFFLTGIPGLEGSHGWISLPFCSIYLMALLGNSTILLIIWSDRTLRDPMFYFLAILSAIDLALSTSSVPRVLGIFWFDAHEISFGACVAQMFLIHTFTGMESAVLLAMAFDRYVAICAPLHYTTVLTPRVLAGIAVGIIMRPILLMLPIVYLTHRLPFCGARIIAHSYCEHMGIAKLACANIRINAIYGLFVASFLILDVALVGISYAYILRAVFRLPSRDARHKALSTCGSHVGVMCIFYTPSLFSFLTHRFGKKIPGYVHILVANLYVVIPPALNPIIYGVRTKQIRERVICAFTLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KVM8</accession>
    <name>A0A8C4KVM8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dual-specificity kinase</fullName>
            <ecNumber evidence="2">2.7.12.1</ecNumber>
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    </protein>
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        <name evidence="15" type="primary">CLK1</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.</text>
    </comment>
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        <property type="gene ID" value="ENSEASG00005000996.2"/>
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    <dbReference type="GeneID" id="106823488"/>
    <dbReference type="KEGG" id="eai:106823488"/>
    <dbReference type="CTD" id="1195"/>
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        <property type="component" value="Chromosome 4"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:protein tyrosine kinase activity"/>
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    <dbReference type="GO" id="GO:0043484">
        <property type="term" value="P:regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="PKc_CLK1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Dual specificity protein kinase CLK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000220">
        <property type="entry name" value="dual specificity protein kinase CLK4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051175">
        <property type="entry name" value="CLK_kinases"/>
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    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
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    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646:SF4">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE CLK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45646">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE DOA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="278"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="20"/>
            <end position="71"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="139"/>
            <end position="162"/>
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    <feature type="region of interest" description="Disordered" evidence="13">
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            <begin position="195"/>
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            <begin position="34"/>
            <end position="45"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="143"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="217"/>
            <end position="229"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="230"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001627.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="601" mass="69944" checksum="9301E604B8638209" modified="2023-09-13" version="2">MGRDEAAPHYLTCFLRASQHGRREALPRSSSRLPAPDAAPAHSAPTRAVPAPPAGTRCSPRPSAETRSQRHRCFRAESRRCGCQLHFVVGARRGWPRLLRGRVANFVAFGLGSLAKTMRHSKRTYCPDWDEKDWDCGKWRSSSSHKRRKRSHSSARENKRCKYNHSKTSDSYYLESRSLNEKDYHSRRYIDEYRNDYNQGCEPGHRHRDHESRYQNHSSKSSGRSGRSSYKSKHRIHHSTSRHHSHGKSHRRKRTRSVEDDEEGHLICQSGDVLSARYEIVDTLGEGAFGKVVECIDHKAGGRHVAVKIVKNVDRYCEAARSEIQVLEHLNTTDPSSTFRCVQMLEWFEHHGHICIVFELLGLSTYDFIKENGFVPFRLDHIRKMAYQICKSVNFLHSNKLTHTDLKPENILFVQSDYTEAYNPKMKRDERTLINPDIKVVDFGSATYDDEHHSTLVSTRHYRAPEVILALGWSQPCDVWSIGCILIEYYLGFTVFPTHDSKEHLAMMERILGPLPKHMIQKTRKRKYFHHDRLDWDEHSSAGRYVSRRCKPLKEFMLSQDAEHELLFDLIEQMLEYDPAKRITLKEALKHPFFYPLKKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KWB0</accession>
    <name>A0A8C4KWB0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Galactokinase</fullName>
            <ecNumber evidence="4">2.7.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Galactose kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">GALK1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the transfer of a phosphate from ATP to alpha-D-galactose and participates in the first committed step in the catabolism of galactose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>alpha-D-galactose + ATP = alpha-D-galactose 1-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13553"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28061"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58336"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:13554"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GHMP kinase family. GalK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.6" evidence="4"/>
    <dbReference type="RefSeq" id="XP_014695325.1">
        <property type="nucleotide sequence ID" value="XM_014839839.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KWB0"/>
    <dbReference type="Ensembl" id="ENSEAST00005003424.2">
        <property type="protein sequence ID" value="ENSEASP00005003129.1"/>
        <property type="gene ID" value="ENSEASG00005002417.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106830305"/>
    <dbReference type="KEGG" id="eai:106830305"/>
    <dbReference type="CTD" id="2584"/>
    <dbReference type="GeneTree" id="ENSGT00950000183187"/>
    <dbReference type="OMA" id="VMPCAIN"/>
    <dbReference type="OrthoDB" id="275179at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004335">
        <property type="term" value="F:galactokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005534">
        <property type="term" value="F:galactose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019402">
        <property type="term" value="P:galactitol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033499">
        <property type="term" value="P:galactose catabolic process via UDP-galactose, Leloir pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061623">
        <property type="term" value="P:glycolytic process from galactose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000040">
        <property type="entry name" value="Galactokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.890:FF:000007">
        <property type="entry name" value="Galactokinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000705">
        <property type="entry name" value="Galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019741">
        <property type="entry name" value="Galactokinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019539">
        <property type="entry name" value="GalKase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006206">
        <property type="entry name" value="Mevalonate/galactokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00131">
        <property type="entry name" value="gal_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457:SF7">
        <property type="entry name" value="GALACTOKINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10457">
        <property type="entry name" value="MEVALONATE KINASE/GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10509">
        <property type="entry name" value="GalKase_gal_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000530">
        <property type="entry name" value="Galactokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00473">
        <property type="entry name" value="GALCTOKINASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00106">
        <property type="entry name" value="GALACTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="11" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Galactokinase N-terminal" evidence="18">
        <location>
            <begin position="19"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="16">
        <location>
            <begin position="108"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="17">
        <location>
            <begin position="292"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00055336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF10509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="392" mass="42085" checksum="B62836BDF03C4B52" modified="2022-01-19" version="1">MAASRQPQAGELLAEARRAFREEFGAEPELAVSAPGRVNLIGEHTDYNQGLVLPMALELLTVLVGSPRADGLVSLLTTSEDADEPRRLQFPLPTAQRSLEPGTPRWANYVKGVIQHYPAAPLPGFSAVVVSSVPLGGGLSSSASLEVATYTFLQQLCPDSGAVAARAQVCQRAEHSFAGVPCGIMDQLIALLGQKGHALLIDCRSLETSLVPLSDPKLAVLITNSNVRHSLGSSEYPLRRRQCEEVARALGKESLREVQLEELEAGRELVSKEGYRRARHVVGEIRRTAQAAAALSRGDYRAFGRLMVESHHSLRDDYEVSCPELDQLVEAALSAPGVYGSRMTGGGFGGCTVTLLEASTASQVMQHIQEQYSSTATFYLSQAADGAKVLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KWQ9</accession>
    <name>A0A8C4KWQ9_EQUAS</name>
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        <submittedName>
            <fullName evidence="10">Neuronal pentraxin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">NPTX2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014711521.2">
        <property type="nucleotide sequence ID" value="XM_014856035.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KWQ9"/>
    <dbReference type="Ensembl" id="ENSEAST00005001777.2">
        <property type="protein sequence ID" value="ENSEASP00005001584.2"/>
        <property type="gene ID" value="ENSEASG00005001282.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106840526"/>
    <dbReference type="KEGG" id="eai:106840526"/>
    <dbReference type="CTD" id="4885"/>
    <dbReference type="GeneTree" id="ENSGT01060000248591"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008306">
        <property type="term" value="P:associative learning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00152">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000012">
        <property type="entry name" value="neuronal pentraxin receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051360">
        <property type="entry name" value="Neuronal_Pentraxin_Related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030476">
        <property type="entry name" value="Pentaxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001759">
        <property type="entry name" value="PTX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277:SF1">
        <property type="entry name" value="NEURONAL PENTRAXIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19277">
        <property type="entry name" value="PENTRAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00354">
        <property type="entry name" value="Pentaxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00895">
        <property type="entry name" value="PENTAXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00159">
        <property type="entry name" value="PTX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00289">
        <property type="entry name" value="PTX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51828">
        <property type="entry name" value="PTX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040297785" evidence="8">
        <location>
            <begin position="18"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="Pentraxin (PTX)" evidence="9">
        <location>
            <begin position="222"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="123"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001584.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="430" mass="47040" checksum="36E03622B861C631" modified="2023-09-13" version="2">MLALLAAGVALAVAAGAQDGLSPGSRFVCTALPPEAARAGCPLPAMPMQGGALSPEEELRAAVLQLRETVVQQKETLGAQREAIRELTGKLARCEGLAGGKAPGRAAGKDTMGDLPRDPSHVVEQLSRSLQTLKDRLESLEHQLRVNVSNAALPSDFREMLQRRLAELERQLLHKVAELEDEKSLLHNETSAHRQKTESALNALLQRVTELERGNSAFKTPDAFKVSLPLRTNYLYGKIKKTLPELFAFTVCLWLRSSASPGIGTPFSYAVPGQANEIVLIEWGNNPIELLINDKVAQLPLFVSDGKWHHICVTWTTRDGMWEAFQDGEKLGTGENLAPWHPIKPGGVLILGQEQDTVGGRFDATQAFVGELSQFNIWDRVLRAQEIVNIANCSTNMPGNIIPWVDNNVDVFGGASKWPVETCEERLLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L192</accession>
    <name>A0A8C4L192_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Thrombospondin-4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">THBS4</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and is involved in various processes including cellular proliferation, migration, adhesion and attachment, inflammatory response to CNS injury, regulation of vascular inflammation and adaptive responses of the heart to pressure overload and in myocardial function and remodeling. Binds to structural extracellular matrix (ECM) proteins and modulates the ECM in response to tissue damage, contributing to cardioprotective and adaptive ECM remodeling. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors and protects myocardium from pressure overload. May contribute to spinal presynaptic hypersensitivity and neuropathic pain states after peripheral nerve injury. May play a role in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury in a NOTCH1-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Sarcoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
            <location evidence="3">Extracellular space</location>
            <location evidence="3">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the thrombospondin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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    <dbReference type="SMR" id="A0A8C4L192"/>
    <dbReference type="Ensembl" id="ENSEAST00005003005.2">
        <property type="protein sequence ID" value="ENSEASP00005002739.2"/>
        <property type="gene ID" value="ENSEASG00005002059.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155227"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016529">
        <property type="term" value="C:sarcoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048266">
        <property type="term" value="P:behavioral response to pain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071603">
        <property type="term" value="P:endothelial cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051451">
        <property type="term" value="P:myoblast migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016525">
        <property type="term" value="P:negative regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001938">
        <property type="term" value="P:positive regulation of endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090023">
        <property type="term" value="P:positive regulation of neutrophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034103">
        <property type="term" value="P:regulation of tissue remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048771">
        <property type="term" value="P:tissue remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd16080">
        <property type="entry name" value="TSP-4cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1080.10:FF:000004">
        <property type="entry name" value="Cartilage oligomeric matrix protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000025">
        <property type="entry name" value="Thrombospondin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000027">
        <property type="entry name" value="Thrombospondin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000002">
        <property type="entry name" value="Thrombospondin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1080.10:FF:000001">
        <property type="entry name" value="Thrombospondin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000262">
        <property type="entry name" value="Thrombospondin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000277">
        <property type="entry name" value="Thrombospondin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000123">
        <property type="entry name" value="Thrombospondin 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.10:FF:000001">
        <property type="entry name" value="thrombospondin-3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1080.10">
        <property type="entry name" value="TSP type-3 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003367">
        <property type="entry name" value="Thrombospondin_3-like_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017897">
        <property type="entry name" value="Thrombospondin_3_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008859">
        <property type="entry name" value="Thrombospondin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024665">
        <property type="entry name" value="TSP/COMP_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046970">
        <property type="entry name" value="TSP/COMP_CC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028974">
        <property type="entry name" value="TSP_type-3_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048287">
        <property type="entry name" value="TSPN-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10199">
        <property type="entry name" value="THROMBOSPONDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10199:SF92">
        <property type="entry name" value="THROMBOSPONDIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11598">
        <property type="entry name" value="COMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02412">
        <property type="entry name" value="TSP_3"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05735">
        <property type="entry name" value="TSP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00210">
        <property type="entry name" value="TSPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58006">
        <property type="entry name" value="Assembly domain of cartilage oligomeric matrix protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103647">
        <property type="entry name" value="TSP type-3 repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51234">
        <property type="entry name" value="TSP3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51236">
        <property type="entry name" value="TSP_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 23" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 22" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0339">Growth factor</keyword>
    <keyword evidence="19" id="KW-0497">Mitogen</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0703">Sarcoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 25" id="KW-0732">Signal</keyword>
    <keyword evidence="15" id="KW-0797">Tissue remodeling</keyword>
    <keyword evidence="18" id="KW-0834">Unfolded protein response</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040315797" description="Thrombospondin-4" evidence="25">
        <location>
            <begin position="27"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="26">
        <location>
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</entry>
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Liu"/>
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                <person name="Liu"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
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        <property type="entry name" value="FORKHEAD BOX PROTEIN P4"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00250">
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        <property type="match status" value="1"/>
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    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
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        </source>
    </evidence>
    <sequence length="686" mass="74549" checksum="B46F5506167D6025" modified="2023-09-13" version="2">MMVESASETIRSAPSGQNGVGSLSGQADGSGGAGAAGTAGGGAGRDAATGSDSNGEMSPAELLHFQQQQALQVARQFLLQQASGLSSPGNNDSKQSASAVQVPVSVAMMSPQMLTPQQMQQILSPPQLQALLQQQQALMLQQLQEYYKKQQEQLHLQLLTQQQAGKQQPKEALGNKQLAFQQQLLQMQQLQQQHLLNLQRQGLVSLQPNQASGPLQTLPQAAVCPSDLPQLWKGEGAPGQPAEDSVKQEGLDLTGTATTATSFAAPPKVSPPLSHHTLPNGQPTVLTPRRDSSSHEETPGSHPLYGHGECKWPGCETLCEDLGQFIKHLNTEHALDDRSTAQCRVQMQVVQQLEIQLAKESERLQAMMAHLHMRPSEPKPFSQPVTVSTADSFPDGLVHPPTSAAAPVTPLRPPGLSSASLHSGGPTRRRSSDKFCSPISSELAQNHEFYKNADVRPPFTYASLIRQAILETPDRQLTLNEIYNWFTRMFAYFRRNTATWKNAVRHNLSLHKCFVRVENVKGAVWTVDEREYQKRRPPKMTGSPTLVKNMISGLSYGALNASYQAALAESSFPLLNSPGMLNPSSASSLLPLSHDEVGAPVEPLPSNGSSSPPRLSPPQYRIPSVAATRCRSKRSLRRRRKTGGPVPHWGPPTPAPRGLRKTGTWRKSWHRERNCPKGLQGQAGQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L3S1</accession>
    <name>A0A8C4L3S1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">glycerol kinase</fullName>
            <ecNumber evidence="5">2.7.1.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">GK</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="5"/>
    <dbReference type="RefSeq" id="XP_014699049.1">
        <property type="nucleotide sequence ID" value="XM_014843563.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L3S1"/>
    <dbReference type="Ensembl" id="ENSEAST00005002493.2">
        <property type="protein sequence ID" value="ENSEASP00005002260.2"/>
        <property type="gene ID" value="ENSEASG00005000539.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106832708"/>
    <dbReference type="KEGG" id="eai:106832708"/>
    <dbReference type="CTD" id="2710"/>
    <dbReference type="GeneTree" id="ENSGT01000000214434"/>
    <dbReference type="OrthoDB" id="5422795at2759"/>
    <dbReference type="UniPathway" id="UPA00618">
        <property type="reaction ID" value="UER00672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07792">
        <property type="entry name" value="ASKHA_NBD_FGGY_GK1-3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000043">
        <property type="entry name" value="glycerol kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000033">
        <property type="entry name" value="glycerol kinase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000577">
        <property type="entry name" value="Carb_kinase_FGGY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042018">
        <property type="entry name" value="GK1-3_metazoan-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005999">
        <property type="entry name" value="Glycerol_kin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01311">
        <property type="entry name" value="glycerol_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000756">
        <property type="entry name" value="PRK00047.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF56">
        <property type="entry name" value="GLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000538">
        <property type="entry name" value="GlpK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00933">
        <property type="entry name" value="FGGY_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="9 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="16">
        <location>
            <begin position="13"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="17">
        <location>
            <begin position="282"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005002260.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="559" mass="61360" checksum="E3A561F1AD3142F1" modified="2023-09-13" version="2">MAATKKAVLGPLVGAVDQGTSSTRFLVFNSKTAELLSHHQVEIKQEFPREGWVEQDPKEILQSVYECIEKTCEKLGQLNIDISNIKAIGVSNQRETTVVWDKLTGEPLYNAVVWLDLRTQSTVENLSKRIPGNNNFVKSKTGLPLSTYFSAVKLRWLLDNVRKVQKAVEEDRALFGTIDSWLIWSLTGGANGGVHCTDVTNASRTMLFNIHSLEWDKELCEFFEIPMKVLPNVRSSSEIYGLMKISHSLKAGALEGVPISGCLGDQSAALVGQMCFQDGQAKNTYGTGCFLLCNTGRKCVFSEHGLLTTVAYKLGRDKPVYYALEGSVAIAGAVIRWLRDNLGIIQTSEEIEKLAKEVGTSYGCYFVPAFSGLYAPYWEPSARGIICGLTQFTNKCHIAFAALEAVCFQTREILDAMNRDCGIPLSHLQVDGGMTNNKILMQLQADILYIPVVKPSMPETTALGAAMAAGAAEGVGVWSLEPEDLSAVTMERFEPQINAEESEIRYSTWKKAVMKSMGWVTTQSSESGDPSIFCSLPLGFFIVSSMVMLIGARYISGIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L3Y1</accession>
    <name>A0A8C4L3Y1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L3Y1"/>
    <dbReference type="Ensembl" id="ENSEAST00005003745.2">
        <property type="protein sequence ID" value="ENSEASP00005003415.2"/>
        <property type="gene ID" value="ENSEASG00005005112.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154646"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="CDD" id="cd20679">
        <property type="entry name" value="CYP4F"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.10.630.10:FF:000005">
        <property type="entry name" value="cytochrome P450 4F22 isoform X2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cyt_P450"/>
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    <dbReference type="InterPro" id="IPR017972">
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    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF198">
        <property type="entry name" value="CYTOCHROME P450 4F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
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        <property type="entry name" value="P450"/>
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    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
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    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040403789" evidence="12">
        <location>
            <begin position="22"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="503"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004586"/>
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            <dbReference type="ARBA" id="ARBA00022617"/>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003415.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="559" mass="63501" checksum="1DFC649D85929859" modified="2023-09-13" version="2">MSVFAWSSPIIFFSWAPGARPSPCVPCRMPWLSLSWLGLRPVAASPWLLLLLVGASWLLARVLAWTYTFYDNCCRLRCFPQPPKWNWFLGHLGLVTPTEQGMRVVTQLVANYPQGFVTWLGPVIPLVNLCHPDMVRTVLSASAAIAPKDMVFYSFLKPWLGDGLLLSGGDKWSRHRRMLTPALHFNILKSYVRIFNDSVNIMHAKWKRLASEGSAHLDMFEHISLMTLDSLQKCVFSFNISTPSEYISAVLELSALVTKRNEQIFLYMDSLYHLTSEGQRFHRVCRLVHDFTDSIIQERRRTLPDQGLDDLLKAKAKVKTLDFIDVLLLAKDEDGKELSDEDIRAEADTFMFGGHDTTASGLSWVLYNLARHPEHQERCRQEVRDLLRDREPKEIEWDDLAQLPFLTMCIKESLRLHPPTVGISQRGTQDIVLPDGRVIPKGALNVKGRSILGKKRGPIFGTHHNPSVWPNPEVYDPFRFDPENTKERSPLAFIPFSAGPRNCIGQTFAMTEMKVVLALTLLSFRVLPAGEEPRRKPELILRAEGGLWLRVEPLSAGPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L7U2</accession>
    <name>A0A8C4L7U2_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ubiquitin associated and SH3 domain containing A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">UBASH3A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L7U2"/>
    <dbReference type="Ensembl" id="ENSEAST00005005571.2">
        <property type="protein sequence ID" value="ENSEASP00005005089.2"/>
        <property type="gene ID" value="ENSEASG00005003787.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160841"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 18"/>
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    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050860">
        <property type="term" value="P:negative regulation of T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="UBA_UBS3A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1240:FF:000016">
        <property type="entry name" value="Ubiquitin-associated and SH3 domain-containing protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000053">
        <property type="entry name" value="Ubiquitin-associated and SH3 domain-containing, A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
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    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051710">
        <property type="entry name" value="Phosphatase_SH3-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING BA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16469:SF7">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED AND SH3 DOMAIN-CONTAINING PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22562">
        <property type="entry name" value="UBA_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="UBA" evidence="4">
        <location>
            <begin position="19"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="626" mass="70208" checksum="48322FC85EF84AC9" modified="2023-09-13" version="2">MAAGETQLYAKVSNKLKGRSTPSLLDSLLGMGFPAHTVLKALAATGRKTVEEASDWLHCHCNDPSLDDPIPQEYALFLCPTGSLLEKLQEFWRESKRQCTKNRAHEVFPHITLCDFFTCEDQKVECLYEALKRAGDRILGSFPSVVPLVLHSSVSYLGFFVNDSPADVIREFAATFATEASVLAGRQPLPAPLCLPDFVDCTVKPCTKQLHLTLAHKFYPHHQRTLEQLARAIHPGHSCQWTAALYSRDMRFVHYQTLKALFQYKPQNERTSSRTVSAAARVRLGISHRTGCRGFLPENYTERASECDTWVKHRTYTFSLAVDLNSRKDGAAGSKRNGEFHPPQTSRSLSSVQALQATIVRKSVLVVRHGERVDQIFGKSWLQQCSTPDGKYYRPDLNFPCSLPRRSNGIKDFENDPPLSSCGIFQSRMAGEALLDSGIRITSVFASPALRCIQTAKHILEELKLEKRIKIRVEPGIFEWTKWEAGKAAPTLMSLEELREANFNVSMDYRPVLPLTSLMPAESYDEYVDRCAMSMERIVSICPEDGVILIVGHGSALDSCTRPLLGLPPRDCGDFAQLVRKIPSLGMCFCEENKEEGKWELVNPPVKTLTHGSNSAFNWRNWILGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L8P3</accession>
    <name>A0A8C4L8P3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Fc epsilon receptor Ia</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">FCER1A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
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                <person name="Zhang"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <property type="component" value="Chromosome 25"/>
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        <property type="entry name" value="Immunoglobulins"/>
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        <property type="entry name" value="HIGH AFFINITY IMMUNOGLOBULIN EPSILON RECEPTOR SUBUNIT ALPHA"/>
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        <property type="entry name" value="IG"/>
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    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040445800" evidence="5">
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            <begin position="25"/>
            <end position="235"/>
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    <feature type="transmembrane region" description="Helical" evidence="4">
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            <begin position="187"/>
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<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L8U6</accession>
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            <ecNumber evidence="8">2.3.1.38</ecNumber>
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        </recommendedName>
    </protein>
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        <name evidence="73" type="primary">FASN</name>
    </gene>
    <organism evidence="73 74">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="73 74" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="73" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="73" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="36">Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="43">
            <text>(2E)-butenoyl-[ACP] + NADPH + H(+) = butanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41812"/>
            <dbReference type="Rhea" id="RHEA-COMP:9627"/>
            <dbReference type="Rhea" id="RHEA-COMP:9628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78453"/>
            <dbReference type="ChEBI" id="CHEBI:78454"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="43">
            <dbReference type="Rhea" id="RHEA:41813"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="67">
            <text>(2E)-decenoyl-[ACP] + NADPH + H(+) = decanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41864"/>
            <dbReference type="Rhea" id="RHEA-COMP:9639"/>
            <dbReference type="Rhea" id="RHEA-COMP:9640"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78467"/>
            <dbReference type="ChEBI" id="CHEBI:78468"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="67">
            <dbReference type="Rhea" id="RHEA:41865"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="50">
            <text>(2E)-dodecenoyl-[ACP] + NADPH + H(+) = dodecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41880"/>
            <dbReference type="Rhea" id="RHEA-COMP:9643"/>
            <dbReference type="Rhea" id="RHEA-COMP:9644"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:65264"/>
            <dbReference type="ChEBI" id="CHEBI:78472"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="50">
            <dbReference type="Rhea" id="RHEA:41881"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="45">
            <text>(2E)-hexadecenoyl-[ACP] + NADPH + H(+) = hexadecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41912"/>
            <dbReference type="Rhea" id="RHEA-COMP:9651"/>
            <dbReference type="Rhea" id="RHEA-COMP:9652"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78481"/>
            <dbReference type="ChEBI" id="CHEBI:78483"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="45">
            <dbReference type="Rhea" id="RHEA:41913"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="46">
            <text>(2E)-hexenoyl-[ACP] + NADPH + H(+) = hexanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41832"/>
            <dbReference type="Rhea" id="RHEA-COMP:9631"/>
            <dbReference type="Rhea" id="RHEA-COMP:9632"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78458"/>
            <dbReference type="ChEBI" id="CHEBI:78459"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="46">
            <dbReference type="Rhea" id="RHEA:41833"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="60">
            <text>(2E)-octadecenoyl-[ACP] + NADPH + H(+) = octadecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41928"/>
            <dbReference type="Rhea" id="RHEA-COMP:9655"/>
            <dbReference type="Rhea" id="RHEA-COMP:9656"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78489"/>
            <dbReference type="ChEBI" id="CHEBI:78495"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="60">
            <dbReference type="Rhea" id="RHEA:41929"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="53">
            <text>(2E)-octenoyl-[ACP] + NADPH + H(+) = octanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41848"/>
            <dbReference type="Rhea" id="RHEA-COMP:9635"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78462"/>
            <dbReference type="ChEBI" id="CHEBI:78463"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="53">
            <dbReference type="Rhea" id="RHEA:41849"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="62">
            <text>(2E)-tetradecenoyl-[ACP] + NADPH + H(+) = tetradecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41896"/>
            <dbReference type="Rhea" id="RHEA-COMP:9647"/>
            <dbReference type="Rhea" id="RHEA-COMP:9648"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78475"/>
            <dbReference type="ChEBI" id="CHEBI:78477"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="62">
            <dbReference type="Rhea" id="RHEA:41897"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>(3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41808"/>
            <dbReference type="Rhea" id="RHEA-COMP:9626"/>
            <dbReference type="Rhea" id="RHEA-COMP:9627"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78451"/>
            <dbReference type="ChEBI" id="CHEBI:78453"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:41809"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>(3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41860"/>
            <dbReference type="Rhea" id="RHEA-COMP:9638"/>
            <dbReference type="Rhea" id="RHEA-COMP:9639"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78466"/>
            <dbReference type="ChEBI" id="CHEBI:78467"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:41861"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>(3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41876"/>
            <dbReference type="Rhea" id="RHEA-COMP:9642"/>
            <dbReference type="Rhea" id="RHEA-COMP:9643"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78470"/>
            <dbReference type="ChEBI" id="CHEBI:78472"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:41877"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>(3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41908"/>
            <dbReference type="Rhea" id="RHEA-COMP:9650"/>
            <dbReference type="Rhea" id="RHEA-COMP:9651"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78480"/>
            <dbReference type="ChEBI" id="CHEBI:78481"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:41909"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>(3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41828"/>
            <dbReference type="Rhea" id="RHEA-COMP:9630"/>
            <dbReference type="Rhea" id="RHEA-COMP:9631"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78457"/>
            <dbReference type="ChEBI" id="CHEBI:78458"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:41829"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>(3R)-hydroxyoctadecanoyl-[ACP] = (2E)-octadecenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41924"/>
            <dbReference type="Rhea" id="RHEA-COMP:9654"/>
            <dbReference type="Rhea" id="RHEA-COMP:9655"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78488"/>
            <dbReference type="ChEBI" id="CHEBI:78489"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:41925"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>(3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41844"/>
            <dbReference type="Rhea" id="RHEA-COMP:9634"/>
            <dbReference type="Rhea" id="RHEA-COMP:9635"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78461"/>
            <dbReference type="ChEBI" id="CHEBI:78462"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:41845"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>(3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:41892"/>
            <dbReference type="Rhea" id="RHEA-COMP:9646"/>
            <dbReference type="Rhea" id="RHEA-COMP:9647"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78474"/>
            <dbReference type="ChEBI" id="CHEBI:78475"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:41893"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="47">
            <text>3-oxobutanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxybutanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41804"/>
            <dbReference type="Rhea" id="RHEA-COMP:9625"/>
            <dbReference type="Rhea" id="RHEA-COMP:9626"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78450"/>
            <dbReference type="ChEBI" id="CHEBI:78451"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="47">
            <dbReference type="Rhea" id="RHEA:41805"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>3-oxodecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxydecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41856"/>
            <dbReference type="Rhea" id="RHEA-COMP:9637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9638"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78464"/>
            <dbReference type="ChEBI" id="CHEBI:78466"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="41">
            <dbReference type="Rhea" id="RHEA:41857"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="63">
            <text>3-oxododecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxydodecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41872"/>
            <dbReference type="Rhea" id="RHEA-COMP:9641"/>
            <dbReference type="Rhea" id="RHEA-COMP:9642"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78469"/>
            <dbReference type="ChEBI" id="CHEBI:78470"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="63">
            <dbReference type="Rhea" id="RHEA:41873"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="64">
            <text>3-oxohexadecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyhexadecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9650"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78478"/>
            <dbReference type="ChEBI" id="CHEBI:78480"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="64">
            <dbReference type="Rhea" id="RHEA:41905"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="55">
            <text>3-oxohexanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyhexanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41824"/>
            <dbReference type="Rhea" id="RHEA-COMP:9629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9630"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78456"/>
            <dbReference type="ChEBI" id="CHEBI:78457"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="55">
            <dbReference type="Rhea" id="RHEA:41825"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>3-oxooctadecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyoctadecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41920"/>
            <dbReference type="Rhea" id="RHEA-COMP:9653"/>
            <dbReference type="Rhea" id="RHEA-COMP:9654"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78487"/>
            <dbReference type="ChEBI" id="CHEBI:78488"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="38">
            <dbReference type="Rhea" id="RHEA:41921"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="65">
            <text>3-oxooctanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxyoctanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41840"/>
            <dbReference type="Rhea" id="RHEA-COMP:9633"/>
            <dbReference type="Rhea" id="RHEA-COMP:9634"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78460"/>
            <dbReference type="ChEBI" id="CHEBI:78461"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="65">
            <dbReference type="Rhea" id="RHEA:41841"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="59">
            <text>3-oxotetradecanoyl-[ACP] + NADPH + H(+) = (3R)-hydroxytetradecanoyl-[ACP] + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:41888"/>
            <dbReference type="Rhea" id="RHEA-COMP:9645"/>
            <dbReference type="Rhea" id="RHEA-COMP:9646"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78473"/>
            <dbReference type="ChEBI" id="CHEBI:78474"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="59">
            <dbReference type="Rhea" id="RHEA:41889"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>a (3R)-hydroxyacyl-[ACP] + NADP(+) = a 3-oxoacyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17397"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="1.1.1.100"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="40">
            <dbReference type="Rhea" id="RHEA:17399"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O</text>
            <dbReference type="Rhea" id="RHEA:13097"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9945"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78827"/>
            <dbReference type="EC" id="4.2.1.59"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:13098"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="56">
            <text>a 2,3-saturated acyl-[ACP] + NADP(+) = a (2E)-enoyl-[ACP] + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22564"/>
            <dbReference type="Rhea" id="RHEA-COMP:9925"/>
            <dbReference type="Rhea" id="RHEA-COMP:9926"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78784"/>
            <dbReference type="ChEBI" id="CHEBI:78785"/>
            <dbReference type="EC" id="1.3.1.39"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="56">
            <dbReference type="Rhea" id="RHEA:22566"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="54">
            <text>a fatty acyl-[ACP] + malonyl-[ACP] + H(+) = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:22836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9916"/>
            <dbReference type="Rhea" id="RHEA-COMP:14125"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78776"/>
            <dbReference type="ChEBI" id="CHEBI:138651"/>
            <dbReference type="EC" id="2.3.1.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="54">
            <dbReference type="Rhea" id="RHEA:22837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>acetyl-CoA + n malonyl-CoA + 2n NADPH + 2n H(+) = a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP(+).</text>
            <dbReference type="EC" id="2.3.1.85"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="48">
            <text>acetyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxobutanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41800"/>
            <dbReference type="Rhea" id="RHEA-COMP:9621"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9625"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78446"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78450"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="48">
            <dbReference type="Rhea" id="RHEA:41801"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="66">
            <text>butanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxohexanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41820"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9628"/>
            <dbReference type="Rhea" id="RHEA-COMP:9629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78454"/>
            <dbReference type="ChEBI" id="CHEBI:78456"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="66">
            <dbReference type="Rhea" id="RHEA:41821"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="61">
            <text>decanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxododecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41868"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9640"/>
            <dbReference type="Rhea" id="RHEA-COMP:9641"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78468"/>
            <dbReference type="ChEBI" id="CHEBI:78469"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="61">
            <dbReference type="Rhea" id="RHEA:41869"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="44">
            <text>dodecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxotetradecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41884"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9644"/>
            <dbReference type="Rhea" id="RHEA-COMP:9645"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:65264"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78473"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="44">
            <dbReference type="Rhea" id="RHEA:41885"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="58">
            <text>hexadecanoyl-[ACP] + H2O = hexadecanoate + holo-[ACP] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41932"/>
            <dbReference type="Rhea" id="RHEA-COMP:9652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78483"/>
            <dbReference type="EC" id="3.1.2.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="58">
            <dbReference type="Rhea" id="RHEA:41933"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="49">
            <text>hexadecanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxooctadecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41916"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9653"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78483"/>
            <dbReference type="ChEBI" id="CHEBI:78487"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="49">
            <dbReference type="Rhea" id="RHEA:41917"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>hexanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxooctanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41836"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9632"/>
            <dbReference type="Rhea" id="RHEA-COMP:9633"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="ChEBI" id="CHEBI:78459"/>
            <dbReference type="ChEBI" id="CHEBI:78460"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:41837"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="57">
            <text>holo-[ACP] + acetyl-CoA = acetyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41788"/>
            <dbReference type="Rhea" id="RHEA-COMP:9621"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78446"/>
            <dbReference type="EC" id="2.3.1.38"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="57">
            <dbReference type="Rhea" id="RHEA:41789"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="52">
            <text>holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="52">
            <dbReference type="Rhea" id="RHEA:41793"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="68">
            <text>octanoyl-[ACP] + malonyl-[ACP] + H(+) = 3-oxodecanoyl-[ACP] + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:41852"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9636"/>
            <dbReference type="Rhea" id="RHEA-COMP:9637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
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            <text>tetradecanoyl-[ACP] + H2O = tetradecanoate + holo-[ACP] + H(+)</text>
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        <reaction evidence="42">
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        <text evidence="1">Lipid metabolism; fatty acid biosynthesis.</text>
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    <dbReference type="PROSITE" id="PS52019">
        <property type="entry name" value="PKS_MFAS_DH"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="24" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="17" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="23" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="25" id="KW-0456">Lyase</keyword>
    <keyword evidence="26" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="22" id="KW-0520">NAD</keyword>
    <keyword evidence="18" id="KW-0521">NADP</keyword>
    <keyword evidence="21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="74" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0702">S-nitrosylation</keyword>
    <keyword evidence="14" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="79"/>
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    <feature type="domain" description="PKS/mFAS DH" evidence="72">
        <location>
            <begin position="916"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="domain" description="Carrier" evidence="70">
        <location>
            <begin position="2204"/>
            <end position="2281"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-terminal hotdog fold" evidence="69">
        <location>
            <begin position="916"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="region of interest" description="C-terminal hotdog fold" evidence="69">
        <location>
            <begin position="1064"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for dehydratase activity" evidence="69">
        <location>
            <position position="956"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for dehydratase activity" evidence="69">
        <location>
            <position position="1113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013191"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00013258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00018769"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022799"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
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            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
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            <dbReference type="ARBA" id="ARBA00022832"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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    <evidence type="ECO:0000256" key="21">
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    <evidence type="ECO:0000256" key="22">
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    <evidence type="ECO:0000256" key="23">
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    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
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    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
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    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
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    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023332"/>
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    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00023351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00023373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00023388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00023394"/>
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    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00023398"/>
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    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00023399"/>
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    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00023401"/>
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    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
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    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="ARBA" id="ARBA00044883"/>
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    </evidence>
    <evidence type="ECO:0000256" key="38">
        <source>
            <dbReference type="ARBA" id="ARBA00047300"/>
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    </evidence>
    <evidence type="ECO:0000256" key="39">
        <source>
            <dbReference type="ARBA" id="ARBA00047394"/>
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    </evidence>
    <evidence type="ECO:0000256" key="40">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="41">
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    </evidence>
    <evidence type="ECO:0000256" key="42">
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    </evidence>
    <evidence type="ECO:0000256" key="43">
        <source>
            <dbReference type="ARBA" id="ARBA00047500"/>
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    </evidence>
    <evidence type="ECO:0000256" key="44">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="45">
        <source>
            <dbReference type="ARBA" id="ARBA00047810"/>
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    </evidence>
    <evidence type="ECO:0000256" key="46">
        <source>
            <dbReference type="ARBA" id="ARBA00047897"/>
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    </evidence>
    <evidence type="ECO:0000256" key="47">
        <source>
            <dbReference type="ARBA" id="ARBA00047953"/>
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    </evidence>
    <evidence type="ECO:0000256" key="48">
        <source>
            <dbReference type="ARBA" id="ARBA00047961"/>
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    </evidence>
    <evidence type="ECO:0000256" key="49">
        <source>
            <dbReference type="ARBA" id="ARBA00048051"/>
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    </evidence>
    <evidence type="ECO:0000256" key="50">
        <source>
            <dbReference type="ARBA" id="ARBA00048281"/>
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    </evidence>
    <evidence type="ECO:0000256" key="51">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="52">
        <source>
            <dbReference type="ARBA" id="ARBA00048404"/>
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    </evidence>
    <evidence type="ECO:0000256" key="53">
        <source>
            <dbReference type="ARBA" id="ARBA00048420"/>
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    </evidence>
    <evidence type="ECO:0000256" key="54">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="55">
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    <evidence type="ECO:0000256" key="56">
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    <evidence type="ECO:0000256" key="57">
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    </evidence>
    <evidence type="ECO:0000256" key="58">
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    </evidence>
    <evidence type="ECO:0000256" key="59">
        <source>
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    <evidence type="ECO:0000256" key="60">
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    <evidence type="ECO:0000256" key="61">
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    <evidence type="ECO:0000256" key="68">
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    </evidence>
    <evidence type="ECO:0000256" key="69">
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    </evidence>
    <evidence type="ECO:0000259" key="70">
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            <dbReference type="PROSITE" id="PS50075"/>
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    </evidence>
    <evidence type="ECO:0000259" key="71">
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    <evidence type="ECO:0000259" key="72">
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    <evidence type="ECO:0000313" key="73">
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            <dbReference type="Ensembl" id="ENSEASP00005008118.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="74">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="2594" mass="281275" checksum="563A7A616CCE580E" modified="2023-09-13" version="2">MPSGGLGTALSLSLSEQLALTEPGGQLLQRMGGQCAEGWTQLSLLCDRAHHVALVGSACPGVKCGQCRLPVSLTERAAMEEVVIAGMSGRLPESENMQEFWANLIGGVDMVTDDDRRWKAGLYGLPRRSGKLKDLSKFDASFFGVHPKQAHTMDPQLRLLLEVTYEAIVDGGINPALLRGTHTGVWVGVSGSEASEALSRDPETLLGYSMVGCQRAMMANRLSFFFDLKGPSITLDTACSSSLLALHNAYQAIRSGECPAAIVGGINVLLKPNTSLQFMKLGMLSPEGACKSFDEAGNGYCRSEAVVAVLLTKKSLARRVYATILNSGTNTDGSKEQGVTFPSGDAQEQLMSSLYKPAGVAPESLEYLEAHGTGTRVGDPQELKGVIRALCATRQDPLLIGSTKSNMGHPEPASGLAALAKVLLSLEHGLWAPNLHFHSPNPEIPALRDGRLQVVDRPLPIRGGNVGINSFGFGGSNVHVILQPNSRLPLVPTPHVALPCLLRASGRTSEAVLGLLEQGRQHSQDLAFVSMLNDIAATPPSAMPFRGYAVLGGEGDAQEVQQVSTGQRPLWFICSGMGAQWRGMGLSLMRLGTFRDSILRSDKAVKPLGLQVSDLLLSADEATFDDTVPGFVSLTAIQIALIDLLTSMGLRPDGIIGHSLGEVACGYADGCVSQEEAILAAYWRGQCIKEANIPPGAMAAVGLSWEECKQRCPPGVVPACHNSKDTVTISGPQAAVSEFVEQLKQEGVFAKEVRTGGLAFHSYFMDSISHTLLQALKKVIREPRPRSARWLSTSIPEAQWQSSLARTFSAEYNVNNLVSPVLFQEALCHVPEHAVVLEIAPHALLQAVLKRGLKPSCTIVPLMKKDHRDNLEFFLSNVGRLHLMGIDVNPNGLFPPVEFPAPRGTPLISPHIKWDHSQTWYVPSAQDFPSGSSCSSSTVYNIDASPESPDHYLVDHRIDGRTLFPATGYLCLVWRTLAHALGENLEQMPVVFEDVTLYQATILPKTGTMPLEVRLLEASHTFEVSENGTLIVSGKVYQWDNPNPKLFDHRDSLDPADPTDPTATFHLAQGDVYKELRLRGYDYGPHFQGILKANLEGNTGQLLWKDNWVTFLDTMLQMSILGSAQRSLRLPTRIAAIHIDPATHQQKVYTLQGEAQAADLVVDSCLSSTVAGGAIISWLHTSLAPRRQQEQLAPTLEKFCFTPHVEGGCLAGSATLQEELQLCKGLAQALQVKVAQQGLKMVVPGLDGAQVPRESPQQGLPRLLAAACQLQLNGNLQLELGQVLAQESPLLCDDPLLSGLLDSPALKACVDTALENMASLRMKVVEVLAGDGHLYSRIPALLTTQPMLQLDYTATDRHAQALKAAQAKLQQHHVAQGQWDPMDPAPSSLGATDLLVCNCAVATLGDPATALGNMAAVLKEGGFLLLHTLLRGYPLGEMVAFLTCPEPQRGRQGLLSQDEWESLFAGASLRLVALKKSFYGSVLFLCRRPAPQDSPIFLPVEDMSFRWVDSLKNILADLSSRPVWLTAVSCSISGVIGMANCLRKEPGGHRIRCILVSNLSNTSPVPKMDPSSSELQKVLQGDLVMNVYRDGAWGAFRHFPLEQDQPEEQTEHAFINVLTRGDLSSIRWVCSPLRHAQHPGPGLQLCTVYYASLNFRDIMLATGKLSPDAIPGKWAARDCMLGMEFSGRDASGKRVMGLVPAEGLATSVLLSQDFLWDVPSCWTLEEAASVPVVYTTAYYSLVVRGRVRRGETVLIHSGSGGVGQAAIAIALSLGCRVFTTVGSAEKRAYLQARFPQLNDTSFANSRDTSFEQHVLRHTAGKGVDLVLNSLAEEKLQASVRCLAQHGRFLEIGKFDLSNNHPLGMAVFLKNVTFHGILLDALFDETSSDWQEVVALVQAGIQDGVVQPLKCTVFPKTQVEDAFRYMAQGKHIGKVVVQVREEEPEAVLQEVKPTRMVALSKTFFPAHKSYVVAGGLGGFGLELAQWLVLRGAQKLVLTSRSGIRTGYQAKQVREWRRQGVQVLVSTCNASSLDGARGLIAEAAQLGPVGGVFNLAMVLRDAMLENQTPELFQDVNKPKYNGTLNLDRVTRETCPELDYFVAFSSVSCGRGNAGQTNYGFANSTMERICEKRRHDGLPGLAVQWGAIGDVGIILEAMGTNDTVIGGTLPQRIASCLEVLDLFLNQPHPVLSSFVLAEKKAAAHSDSGSQQDLVKAVAHILGIQDLATINLDSSLVDLGLDSLMGIEVRQTLEREHDMVLSMRDIQQLTLRKLQELSSQTCMADELAAPTPKEDGLTRQQAQLNLSTLLVNPEAPTLTRLNSVQSSERPLFLVHPIEGSITVFHSLATKLSIPTYGLQCTKAAPLDSIQSLAAYYIECIRQVQPEGPYRIAGYSYGACVAFEMCSQLQAQQSSAPAHNSLFLFDGSHAYVLAYTQSYRAKLTPGCEAEAETEAMCFFIQQFTDAEHSRVLEALLPLKGLEERVVAAVDLIVQNHAGLDRRVLSFAARSFYYKLRAAEQYMPRATYHGNVTLLRAKTGSSYGEDLGADYNLSQVCDGKVSVHIIEGDHRTLLEGSGLESILSIIHSSLAEPRVSVREG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L9W8</accession>
    <name>A0A8C4L9W8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cytochrome b5 reductase 4</fullName>
            <ecNumber evidence="4">1.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Flavohemoprotein b5/b5R</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">cb5/cb5R</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">CYB5R4</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>2 Fe(III)-[cytochrome b5] + NADH = 2 Fe(II)-[cytochrome b5] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46680"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_014693556.1">
        <property type="nucleotide sequence ID" value="XM_014838070.3"/>
    </dbReference>
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        <property type="term" value="P:insulin secretion"/>
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        <property type="term" value="P:response to antibiotic"/>
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        <property type="term" value="P:superoxide metabolic process"/>
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    <dbReference type="CDD" id="cd06183">
        <property type="entry name" value="cyt_b5_reduct_like"/>
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    <dbReference type="CDD" id="cd06490">
        <property type="entry name" value="p23_NCB5OR"/>
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        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
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    <sequence length="520" mass="59257" checksum="9009F9FE911EB1D2" modified="2023-09-13" version="2">MLNVPSQSFPAPSSQQRVVAGGRSKVPLKQGRSLMDWIRLTKSGKDLTGLKGRLIEVTEEELKKHDKKDDCWICIRGFVYNVSPYMEYHPGGEDELMRAAGSDGTDLFDQVHRWVNYESMLKECLVGRMAVKPAAPKDYHEEKKVLNGMLPKSQATDTLAREGPSSPSYDWFQTDSLVTIVIYTKQKDITLDSVIVDHQDDSFRAETIIKDYSYLINIELSHAVQEDFSVRIVENVGKIEIVLQKKENTSWKCLGHPLENHNSLVPKKDAGLFYRQCQLISKEDVTHDTRLFCFMLPPSTHLQVPIGQHVYLKLTVTDTEIVKPYTPVSDSLLSEFKEPALPNNKYIYFLIKIYPAGLFTPELDHLQIGDFVFVSNPEGNFKTSQFQELEDLFLLAAGTGFTPMVKLLNYALTNIPSLRKVKLMFFNKTEDDIIWRSQLEELTFKDKRFDVEFVLSAPISEWNGKQGHVSPALLSEFLKRSSDKSKVLICICGPTPFTEQGIKFLHELNFSKDEIHSFTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LCS5</accession>
    <name>A0A8C4LCS5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">IF rod domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the intermediate filament family.</text>
    </comment>
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        <property type="component" value="Chromosome 13"/>
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        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Type I keratin 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF97">
        <property type="entry name" value="KERATIN, TYPE I CUTICULAR HA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 4" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="6">
        <location>
            <begin position="56"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="60"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="166"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="265"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009244.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="404" mass="45992" checksum="B740FA21F70793F4" modified="2023-09-13" version="2">MSYSCFLPSLSCRSTCSSRPCVPPSCHGCTLPGACNIPATVGSCSWFCEGSFNGNEKETMQFLNDRLASYLEKVRQLERENAELESRIRERCQQQEPEVCPNYQSYFRTIEELQQKILVSKSENARLVVQVDNAKLAADDFRTKYQTELGLRQLVESDINGLRRILDELTLCRSDLEAQVESLKEELISLKQNHEQEVNSLRSQLGDRLNVEVDAAPTVDLNRVLNETRSQYEALVETNRRDVEEWFIKQTEELNRQVVSSSEQLLSYQAEIIELRRTVNALEIELQAQHNLRDSLENTLTETEARYSSQLSQVQGLITNVESQLAEIRSDLERQNQEYQVLLDVRARLESEINTYRGLLESEDCKLPSNPCATTNAGDRSCITNPCTPCVPRPRCGPCNTFGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LCT7</accession>
    <name>A0A8C4LCT7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Serine beta-lactamase-like protein LACTB, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LACTB</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase S12 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014706276.2">
        <property type="nucleotide sequence ID" value="XM_014850790.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LCT7"/>
    <dbReference type="Ensembl" id="ENSEAST00005007991.2">
        <property type="protein sequence ID" value="ENSEASP00005007325.2"/>
        <property type="gene ID" value="ENSEASG00005005348.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837288"/>
    <dbReference type="KEGG" id="eai:106837288"/>
    <dbReference type="CTD" id="114294"/>
    <dbReference type="GeneTree" id="ENSGT00390000001062"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019216">
        <property type="term" value="P:regulation of lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.710.10:FF:000015">
        <property type="entry name" value="Serine beta-lactamase-like protein LACTB, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.710.10:FF:000016">
        <property type="entry name" value="serine beta-lactamase-like protein LACTB, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.710.10">
        <property type="entry name" value="DD-peptidase/beta-lactamase superfamily"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001466">
        <property type="entry name" value="Beta-lactam-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012338">
        <property type="entry name" value="Beta-lactam/transpept-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052794">
        <property type="entry name" value="Mito_Ser_Protease_LACTB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46520">
        <property type="entry name" value="SERINE BETA-LACTAMASE-LIKE PROTEIN LACTB, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46520:SF1">
        <property type="entry name" value="SERINE BETA-LACTAMASE-LIKE PROTEIN LACTB, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00144">
        <property type="entry name" value="Beta-lactamase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56601">
        <property type="entry name" value="beta-lactamase/transpeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Beta-lactamase-related" evidence="11">
        <location>
            <begin position="119"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-lactamase-related" evidence="11">
        <location>
            <begin position="309"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="65"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="244"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="251"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007325.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="556" mass="61759" checksum="2CEF3D12C49ADC22" modified="2023-09-13" version="2">MYRLLSAVTARATTPGGWAWGSGRRGAHQRAGLPPLGPGWVGGLGLGLGLALGVKLVGGLRGAAPSPLPTAPDPEASPQTEPLPPQELSLAPWSPQTPAPPHSRRFARAIESSRDLLHRIKDEVGAPGIVVGVSVDGTEVWSEGLGYADVENRVPCKPETVMRIASISKSLTMVALAKLWEAGKLDLDVPVQHYVPEFPEKEYEGEKVSVTTRLLISHLSGIRHYEKDMKKVKEEKAYKALKMMKGTMESDQERELKEKGGKSNEKNDFAKAKIEQDNEAKGRNSKPGKKKNDFEQGELYLKEKFENSIESLRLFKNDPLFFKPGSQFLYSTFGYTLLAAIVERASGYKYLDYMQKIFHDLDMLTTVQEENEPVIYNRARFYVYNKKRRLVNTPYVDNSYKWAGGGFLSTVGDLLKFGNAMLYGYQVGLFKNSNENLLPGYLRPETMVMIWTPVPNTEMSWDKEGKYAMAWGVVEKKQTYGSCRKQRHYASHTGGAVGASSVLLVLPEELDAETIHNKVPPRGIVVSIICNMQSVGLNSTALKIALEFDKDRSDIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LDD6</accession>
    <name>A0A8C4LDD6_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Myosin IE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">MYO1E</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LDD6"/>
    <dbReference type="Ensembl" id="ENSEAST00005010323.2">
        <property type="protein sequence ID" value="ENSEASP00005009503.2"/>
        <property type="gene ID" value="ENSEASG00005006777.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157461"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005903">
        <property type="term" value="C:brush border"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="C:clathrin-coated endocytic vesicle"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0016459">
        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:calmodulin binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:microfilament motor activity"/>
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        <property type="term" value="F:phosphatidylinositol binding"/>
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        <property type="term" value="P:actin filament organization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:endocytosis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:glomerular basement membrane development"/>
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        <property type="term" value="P:glomerular filtration"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001701">
        <property type="term" value="P:in utero embryonic development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048008">
        <property type="term" value="P:platelet-derived growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072015">
        <property type="term" value="P:podocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035166">
        <property type="term" value="P:post-embryonic hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001570">
        <property type="term" value="P:vasculogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01378">
        <property type="entry name" value="MYSc_Myo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.820:FF:000001">
        <property type="entry name" value="Myosin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.4820:FF:000004">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.530:FF:000007">
        <property type="entry name" value="Myosin IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000101">
        <property type="entry name" value="Slow myosin heavy chain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000072">
        <property type="entry name" value="Unconventional Myosin IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.720:FF:000010">
        <property type="entry name" value="Unconventional myosin-Ie"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.4820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.530">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.720">
        <property type="entry name" value="Myosin VI head, motor domain, U50 subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001609">
        <property type="entry name" value="Myosin_head_motor_dom-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010926">
        <property type="entry name" value="Myosin_TH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036072">
        <property type="entry name" value="MYSc_Myo1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140">
        <property type="entry name" value="MYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13140:SF341">
        <property type="entry name" value="UNCONVENTIONAL MYOSIN-IE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00063">
        <property type="entry name" value="Myosin_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06017">
        <property type="entry name" value="Myosin_TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00193">
        <property type="entry name" value="MYOSINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00242">
        <property type="entry name" value="MYSc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51456">
        <property type="entry name" value="MYOSIN_MOTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51757">
        <property type="entry name" value="TH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="8 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7 11" id="KW-0518">Myosin</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Myosin motor" evidence="14">
        <location>
            <begin position="19"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="domain" description="TH1" evidence="15">
        <location>
            <begin position="730"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="13">
        <location>
            <begin position="1051"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="region of interest" description="Actin-binding" evidence="11">
        <location>
            <begin position="569"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="919"/>
            <end position="1056"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="925"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="978"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="999"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="1035"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="112"/>
            <end position="119"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009503.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1088" mass="124633" checksum="143FE60DA35C18D3" modified="2023-09-13" version="2">MGSKGAYRYHWQSHNVKHSGVDDMVLLSKITENAIVENLKKRYMDDYIFTYIGSVLISVNPFKQMPYFGEKEIEMYQGAAQYENPPHIYALADSMYRNMIIDRENQCVIISGESGAGKTVAAKYIMSYISRVSGGGPKVQHVKDIILQSNPLLEAFGNAKTVRNNNSSRFGKYFEIQFSPGGEPDGGKISNFLLEKSRVVMRNPGERSFHIFYQLIEGASAEQKHSLGITSMDYYYYLSLSGSYKVDDIDDRREFQETLHAMNVIGIFAEEQTLVLQIVAGVLHLGNISFKEVGNYAAVESEEFLAFPAYLLGINQDRLKEKLTSRQMDSKWGGKSESIHVTLNVEQACYTRDALAKALHARVFDFLVDSINKAMEKDHEEYNIGVLDIYGFEIFQKNGFEQFCINFVNEKLQQIFIELTLKAEQEEYVQEGIKWTPIEYFNNKIVCDLIENKVNPPGIMSILDDVCATMHAVGEGADQTLLQKLQMQIGNHEHFNSWNQGFIIHHYAGKVSYDMDGFCERNRDVLFMDLIELMQSSELPFIKSLFPENLQADKKGRPTTAGSKIKKQANDLVNTLMKCTPHYIRCIKPNETKKPKDWEESRVKHQVEYLGLKENIRVRRAGYAYRRIFQKFLQRYAILTKATWPSWKGDEKQGVLHLLQSVNMDSDQFQLGRSKVFIKAPESLFLLEEMRERKYDGYARVIQKSWRKFVARKKYVQMREEASDLLLNKKERRRNSINRNFIGDYIGMEEHPELQQFVGKREKIDFADTVTKYDRRFKGVKRDLLLTPKCLYLIGREKVKQGPDKGLVKEVLKRRIEVERILSVSLSTMQDDIFILHEQEYDSLLESVFKTEFLSLLAKRYEEKTQKQLPLKFSNTLELKLKKENWGPWSAGGSRQVQFHQGFGDLAILKPSNKVLQVSIGPGLPRNSRPTRRNTTQNRSYPSGTQNANYPMRAAPPPPGYHQNGVIKNQFVPPSHAPGNQRSNQKSLYTSMARPPLPRQQSTGSDRVSQAPESLDFLKVPDQGAAGVRRQTTSRPPPAGGRPKPQPKPKPQVPQCKALYAYDAQDTDELSFNANDIIDIIKEAPPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LE85</accession>
    <name>A0A8C4LE85_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="34" type="primary">SCN5A</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="34 35" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="27">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Cell membrane</location>
            <location evidence="24">Sarcolemma</location>
            <location evidence="24">T-tubule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 27">Cell membrane</location>
            <topology evidence="3 27">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="26">Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.5/SCN5A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="27">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005011627.2">
        <property type="protein sequence ID" value="ENSEASP00005010688.2"/>
        <property type="gene ID" value="ENSEASG00005007552.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161691"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00024012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00060796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="RuleBase" id="RU361132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="Pfam" id="PF06512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="Pfam" id="PF11933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="Pfam" id="PF24609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005010688.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="2020" mass="228201" checksum="386A7BCE04A38ECF" modified="2023-09-13" version="2">MADFLLPRGTSSFRRFTRESLAAIEKRMAEKQARGLAASQESREGLPEEEAPRPQLDLQASKKLPDLYGNPPRELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPIRRAAVKILVHSLFSMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIVMAYTTEFVDLGNVSALRTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTALNDTNGSVEADGLVWESLDLYLNDPENYLLKNGTSDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMEMLKKEHEALTIRGVDTVSRSSLEMSPLAPVTTRERRSKRRKRMSSGTEECGDDRFPKSNSEDGPRAMNRLSISHGLSRTSMKQRSSRGSIFTFRRRDLGSETDFADDENSTAGDSESHRTSLLVPWPLRRPSALGQPSPGTSAPSHALNGKRNSTVDCNGVVSLLGAGDPEATSPGSRLLRPIMLERPPDTTTPSEEPGRPQMLSPQAPCADGFEEPGERQRALSAVSVLTSVLEELEESHRKCPPCWTRFAQRYLIWECCPLWMSIKQRVKFMVMDPFADLTITLCIVINTLFMALEHYNMTTEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSELRYRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFVKRTTWDFCCGLLQQRPQKPAALATRDELPSCIATSGSPQPPETEKAPPARKETRFEEGNRPGQGTPGDPEPVCVPIAMAESDTDDQEEEEENSLGTEEESSKQQESHPVSGGQEALPEPRAWSQVSETTSSEAYEARAAPAERRQQRQAEPPAPGCSETQEDSYSEGSTADMTNTADLMEQIPDLGEDVKDPEDCFTEGCVRRCPCCTVDTTQGPGKVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLISLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSDCESFNVTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGFEEQPQWEYNLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFLFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKVNILAKINLLFVAIFTGECIIKMTALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPSCDPNLPNSNGSRGNCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYSALSDFADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSATIIQRAFRRHLLQRSMKHASFLFRQQAGSSGLSEEDAPEQEGLIAYMMNENFSRRHGPPSSSSISSSSFPPSYDSVTRATSDNLQARVSDYSRSEDLADFPPSPDRDRESIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LEN7</accession>
    <name>A0A8C4LEN7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">AT-rich interactive domain-containing protein 3</fullName>
            <shortName evidence="9">ARID domain-containing protein</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ARID3B</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Transcription factor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8 9">Heterodimer with ARID3A. Interacts with unphosphorylated RB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LEN7"/>
    <dbReference type="Ensembl" id="ENSEAST00005007521.2">
        <property type="protein sequence ID" value="ENSEASP00005006885.2"/>
        <property type="gene ID" value="ENSEASG00005005082.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156052"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd16879">
        <property type="entry name" value="ARID_ARID3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.60:FF:000008">
        <property type="entry name" value="Putative AT-rich interactive domain-containing protein 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045147">
        <property type="entry name" value="ARI3A/B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023334">
        <property type="entry name" value="REKLES_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15348:SF3">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15348">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN ARID DOMAIN- CONTAINING PROTEIN DEAD RINGER PROTEIN B-CELL REGULATOR OF IGH TRANSCRIPTION BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51486">
        <property type="entry name" value="REKLES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="7 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ARID" evidence="11">
        <location>
            <begin position="221"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="REKLES" evidence="12">
        <location>
            <begin position="421"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="89"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="372"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="526"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="173"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="526"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006885.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="563" mass="61149" checksum="807B3C86722FCF7F" modified="2023-09-13" version="2">MEPQQQQKQQQQQQQQPPPQPPPAHLAALQMDTREKQGQQMREAQFLYAQKLVSQQTLFSATPGKPSGSPALGPLARVPQAPAVTRVFERSSVNSEPEEEEGCLEDEEGDDEVREVAEKEAQAASKYFHAQKLARQDPRAAPVSGLLPAPGLPPHGQQAKEDHTKDASKAPPSVSTAGQPSWNLDEQLKQNGGLAWSDDADGSRGREISRDFAKLYELDGDPERKEFLDDLFVFMQKRGTPINRIPIMAKQILDLYMLYKLVTEKGGLVEIINKKIWREITKGLNLPTSITSAAFTLRTQYMKYLYAYECEKKALSSPAELQAAIDGNRREGRRPSYSSSLFGYSPTAAAAGAPALLSPPKIRFPILGLGSSSGTNTSSPRISPATTLRKGDGVSVTTVPVPNRLAVPVTLAGQQATTRTATLEQLRERLESGEPPEKKTSRLSEEEQRLVQQAFQRNFFSMARQLPMKIRINGRAEDRSEASAAALNLTTSSIGSINMSVDIDGTTYTGVLFAQKPVVHLIAGSAPQSIGSSASSSSSSHCSPSPTSSRGTPSAEPSTSWSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LF50</accession>
    <name>A0A8C4LF50_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">tryptase</fullName>
            <ecNumber evidence="5">3.4.21.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PRSS33</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa, but with more restricted specificity than trypsin.</text>
            <dbReference type="EC" id="3.4.21.59"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.59" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4LF50"/>
    <dbReference type="Ensembl" id="ENSEAST00005009091.2">
        <property type="protein sequence ID" value="ENSEASP00005008342.2"/>
        <property type="gene ID" value="ENSEASG00005006015.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162519"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000039">
        <property type="entry name" value="Brain-specific serine protease 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253:SF58">
        <property type="entry name" value="SERINE PROTEASE 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24253">
        <property type="entry name" value="TRANSMEMBRANE PROTEASE SERINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040321530" description="tryptase" evidence="7">
        <location>
            <begin position="20"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="35"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00050838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00066748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008342.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="278" mass="29658" checksum="936E45F9FE4E43BB" modified="2023-09-13" version="2">MRGASCLQVLLLMLLGAAGTLQSAACGQPRVSSRIVGGQDARDGEWPWQASIQHRGAHVCGGSLIAPQWVLTAAHCFPRRALPSEYHVRLGALHLGAASPRALSAPVRRVLLPPDYSEDGARGDLALLQLRRPVPLSAQVQPVCLPEPGSRPPPGTPCWVTGWGSLHPGVPLPEWRPLQGVRVPLLDVRACDRLYHLGTNVPRAERIVLPGNLCAGYIEGHKDACQGDSGGPLTCVKSGRWVLVGVVSWGKGCALPNRPGVYTNVATYSPWIQAHLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LG46</accession>
    <name>A0A8C4LG46_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Solute carrier family 35 member F5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SLC35F5</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Putative solute transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC35F solute transporter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014714487.1">
        <property type="nucleotide sequence ID" value="XM_014859001.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LG46"/>
    <dbReference type="Ensembl" id="ENSEAST00005011487.2">
        <property type="protein sequence ID" value="ENSEASP00005010555.1"/>
        <property type="gene ID" value="ENSEASG00005007490.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106842465"/>
    <dbReference type="KEGG" id="eai:106842465"/>
    <dbReference type="CTD" id="80255"/>
    <dbReference type="GeneTree" id="ENSGT00390000005949"/>
    <dbReference type="OMA" id="MYGVYTI"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051:SF0">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35, MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="266"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="301"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="359"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="424"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="453"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005010555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="523" mass="58906" checksum="679E4A83562C5D34" modified="2022-01-19" version="1">MVPPRRHRGAGRPGVLSSSPPFRLRFAKFSGIALDLRKAFKIRLQMVCVFIMNRMNSQSSGFAQRRRMALGIVMLLVVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYLLGFIVWKPWRQQCTRGFRGKHATFFADAEGYFAACTTDTTMNSSLSEPLYVPVKFHDLPSEKPENTNVDAQKTPKKSRVRFSNIMEIRQLPSSHALDAKLSRMSHPAVKEQEPLLKTVGKLTATQVAKISFFFCFVWFLANFSYQEALSDTQVAIVNILSSTSGLFTLILAAVFPSNSGDRFTLSKLLAVILSIGGVVLVNLSGSEKSSGRDTIGSIWSLVGAMLYAVYIVMIKRKVDREDKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFEDFEFPNKVVLMCIIINGLIGTVLSEFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMKKVQFSWLFFAGAIPVFFSFFIATLLCHYNNWDPVMVGIRRVFAFICRKHRIQRVPEDSEQCESLIPMHGVSQEDGAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LK59</accession>
    <name>A0A8C4LK59_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Zinc finger protein 175</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ZNF175</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_044759757.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LK59"/>
    <dbReference type="Ensembl" id="ENSEAST00005013328.2">
        <property type="protein sequence ID" value="ENSEASP00005012269.2"/>
        <property type="gene ID" value="ENSEASG00005008565.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822344"/>
    <dbReference type="CTD" id="7728"/>
    <dbReference type="GeneTree" id="ENSGT00940000163075"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001882">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000384">
        <property type="entry name" value="Zinc finger protein 550"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000754">
        <property type="entry name" value="Zinc finger protein 585A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001396">
        <property type="entry name" value="Zinc finger protein 585A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000028">
        <property type="entry name" value="zinc finger protein 90 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050752">
        <property type="entry name" value="C2H2-ZF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384">
        <property type="entry name" value="FINGER PUTATIVE TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384:SF242">
        <property type="entry name" value="ZINC FINGER PROTEIN 628"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="15">
        <location>
            <begin position="98"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="349"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
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    <feature type="domain" description="C2H2-type" evidence="14">
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    <feature type="domain" description="C2H2-type" evidence="14">
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    <feature type="domain" description="C2H2-type" evidence="14">
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    <feature type="domain" description="C2H2-type" evidence="14">
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    <feature type="region of interest" description="Disordered" evidence="13">
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            <begin position="754"/>
            <end position="773"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
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            <begin position="19"/>
            <end position="41"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
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    <evidence type="ECO:0000256" key="1">
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    <evidence type="ECO:0000256" key="2">
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    <evidence type="ECO:0000256" key="12">
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    <evidence type="ECO:0000256" key="13">
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    <evidence type="ECO:0000259" key="14">
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    <evidence type="ECO:0000259" key="15">
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    <evidence type="ECO:0000313" key="16">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="784" mass="89935" checksum="FDAAE2F785435F74" modified="2023-09-13" version="2">MRRGDLWGLPGVSAEPLGQATSLRPQDLPSQPNLTSPSRSHWSLQMPTQFWAPGKSGVVSKRDREVKSRGDMSAGVNLPPRPQVLGPAEQDRSCEGSVSFEDVTMDFSREEWQQLDPAQRRLYQDVMLEIYSHLFSVGYHIPIPEIIFRMEKGKEPWIGKAKLLPHQRRQEGDLGLETPPQETSEKASFHNDMAVEVTRDRSWCSILRELWKDTDHTKRDQQNQNRSSDHGVFRSKKTLKTEKDCEYKDPGKISHVRPDHVSSQKRPHIRYSFAKSLKPNLEGNHQNQSNTTKPLDEIVGSGQLFTHSSSSASGKNIHTGENFCESNQCTKVRGHKQSLTQHQIQEKPDKCTECGRDFAQKSHLLEQQRFHSVENLQECSKCGKAFTPQPKLNVCLTDHTGHVPYICKECGKVFVQKSELSTHQKTHTRKKPHKCPECGKAFFQMLSLFRHQRTHTREKLYKCSECGKAFSQNSTLNIHQKIHTGEREYACSDCGKAFTQKSTLSLHQRIHSGEKSYVCIECGQAFIQKAHLTVHQRSHTGEKPFECHNCGKSFISKSQLDIHHRIHTGEKPYECNDCGKAFTQKSHLNIHQKIHTGERHHVCSACGKAFNQKSILSMHQRTHTGEKPYKCSDCGKAFTSKSQFREHQRIHTGEKPYVCTECGKAFNGRSNFHKHQITHTRERTYACYKCGNTFLQKSELITHQRIHIGEKPYECRDCGKSFSKKPQLRVHQRIHTGERPYACSKCGKTFNNRSNFNKHQTTHTKDKSYRSSYSAERLYPEINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LMA6</accession>
    <name>A0A8C4LMA6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106841863</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LMA6"/>
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        <property type="protein sequence ID" value="ENSEASP00005013162.2"/>
        <property type="gene ID" value="ENSEASG00005009189.2"/>
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    <dbReference type="GeneID" id="106841863"/>
    <dbReference type="KEGG" id="eai:106841863"/>
    <dbReference type="GeneTree" id="ENSGT01040000240406"/>
    <dbReference type="OrthoDB" id="9007674at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="FunFam" id="1.20.1070.10:FF:000005">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
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    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
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    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
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    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="27"/>
            <end position="50"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="62"/>
            <end position="80"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="100"/>
            <end position="122"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="134"/>
            <end position="156"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="198"/>
            <end position="217"/>
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        <location>
            <begin position="238"/>
            <end position="262"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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            <end position="293"/>
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        <location>
            <begin position="43"/>
            <end position="291"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005013162.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="313" mass="34783" checksum="B49488078EEB5652" modified="2023-09-13" version="2">MGQGNKSQIWVREFILLGLSSDWVTQVSLFVLILAMYVVTTVGNVLILALIRLDSRLHTPMYFFLSVLSFVDLCYSNSFAPQMLAHFLSAQKSIPFHSCVLQLYVSLALGGSEFFLLGAMAYDRYVAVCHPLHYIVIMHGGLCLGLAAGCLVAGFMNSLMETIITFRLPLCHNVINHFACEILAVLQLACVDISFNKIMVAISGFLLIMLPCSLVLFSYGRIVAAILRIRSTQGRRKAFGTCASHLTVVCMCFGAAIFTYLRPQSASSMEEEKMVALFYAVVAPMLNPLIYSLRNKEVMAALQKVSEKFRDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LVY8</accession>
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        <recommendedName>
            <fullName evidence="2">Protein KRI1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">KRI1</name>
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    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the KRI1 family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A8C4LVY8"/>
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        <property type="gene ID" value="ENSEASG00005010499.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 20"/>
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        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018034">
        <property type="entry name" value="Kri1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024626">
        <property type="entry name" value="Kri1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490:SF5">
        <property type="entry name" value="PROTEIN KRI1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490">
        <property type="entry name" value="ZINC FINGER, ZZ TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05178">
        <property type="entry name" value="Kri1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF12936">
        <property type="entry name" value="Kri1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kri1-like C-terminal" evidence="4">
        <location>
            <begin position="497"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="29"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="83"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="126"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="248"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="312"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="431"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="591"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="52"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="151"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="326"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12936"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LYQ6</accession>
    <name>A0A8C4LYQ6_EQUAS</name>
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            <fullName evidence="15">Stabilin-1</fullName>
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        <alternativeName>
            <fullName evidence="16">Fasciclin, EGF-like, laminin-type EGF-like and link domain-containing scavenger receptor 1</fullName>
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    </protein>
    <gene>
        <name evidence="24" type="primary">STAB1</name>
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    <organism evidence="24 25">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="13">Acts as a scavenger receptor for acetylated low density lipoprotein. Binds to both Gram-positive and Gram-negative bacteria and may play a role in defense against bacterial infection. When inhibited in endothelial tube formation assays, there is a marked decrease in cell-cell interactions, suggesting a role in angiogenesis. Involved in the delivery of newly synthesized CHID1/SI-CLP from the biosynthetic compartment to the endosomal/lysosomal system.</text>
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        <text evidence="14">Interacts with CHID1.</text>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
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    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00065563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005019098.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="2633" mass="281690" checksum="6E95AF47D371D926" modified="2023-09-13" version="2">MCDSRPQLGSMRTGPMVGDSAESRPGTVDLNTDELNNLRAHSPRRLPRLCPGQHARQPPSAMAGPRGLLLSLLAFCLAGSGFVMGQKVLSRRCDVKTKFVTHMPCTLCPIIKKQTCPPGWLREFPGKISQDCRYEVQLGDSLVSMSGCSLECWKDVVQKACCPGYWGSQCYECPGGAKTPCNGHGTCLDGIDRNGTCVCQENFSGSACQDCQDPNRFGPDCQSVCSCVHGVCRHGPLGDGSCLCFAGYTGPRCDQELPVCQALHCPQNAQCSAEAPTCSCLPGHTQQGSECRAPDPCRPSPCSPLAQCSVSPRGQAECRCPENYHGDGMVCLPQDPCTTNHGGCPSNSTLCLYQRPGKASCSCKPGLVSINHNASTGCFAYCLPNSCDRSATCQVTPDGKTSCVCKEGEVGDGRACYGHLLHEVQKASQTSTAFLRLRIALAMLDQGCREVLTTSGPFTVLVPSISSIFPRTMNASIAQQLCKQHIIAGQHILEELGTQHTHKWWTLAGQEITVTFNRFMRKYTYKYKDQPQQTFTIHKANYPAVNGIFHVVTALRWQPPTELPEDPKRTISQILASTEAFSRFETILENCGLPSILDGPGPFTVFAPSNEAVDRLRDGRLIYLFTAGLSKLQELVRYHIYSHGQLTIEKLISKGRVLTMANQVLAVNISEEGRILLGPEGVPLRRVDVLAANGVIHMLEGILLPPTILPILPKHCNEEQHKIVAGSCVDCQALNTSTCPPNSVMLDIFPEECVYTYDPSGLNVLKKGCARYCNQTILKPGCCKGFFGPDCAQCPGGFSNPCYGKGNCSDGVRGNGACLCFPDYKGIACHICSNPNKHGDQCQEDCGCVHGLCDNRPGSGGVCQSGTCAPGFSGRFCNESTGSCGPTEQAQHCHLHARCVTQGRVARCLCLDGFEGDGFSCTPSNPCSHPDRGGCSENAECVPGTMGTHHCTCHKGWSGDGRVCVAIDECELDARGGCHADALCSYVGPGQSRCTCKLGFAGDGYMCSPIDPCRAGNGGCHDLATCRAVGGGQRVCTCPPGYGGDGLSCYGDIFQELEANAHFSVFYQWIKSAGITLPADSRVTALVPSESAIRRLSPEDQAFWLQPRMLPQLVRAHFLQGSLSEEELARLGGQDVATLSPTTRWEIHNISGRVWVQNASVDVADLLATNGVLHVLSQVLLPPKGAMPVGQGLLQRLDSVPAFRLFRELLQHHGLVPQIEAATAYTIFVPTNHSLEVQGNRSSLDADTVRHHVVLGEALSVEALQRGGHRNSLLGPAHWLVFYNHSGQPEVNHVPLEGPLLDAPGGSLFGLSGVLTVGSSRCLHTHAEALREKCINCTRKFRCTQGFKLEDTPKKSCVYQSGYSFSQGCSYTCAKKIQVPDCCPGFFGTLCEPCPGGLGGVCSGHGQCQDRLLGSGECRCQEGFHGTACEMCELGRYGPNCTGVCDCAHGLCQEGLRGDGSCVCSVGWQGLRCDQKITGLQCPKKCDPNANCVQDSATAPACICAAGYSGNGIYCSEVDPCAQDHGGCSPHANCTKVAPGQRTCTCQDGYTGDGELCQEVNSCLVHHGGCHLHAECIPTGPQQVSCSCREGYSGDGIRTCELLDPCSQNNGGCSPYAVCKSIGDGQRTCTCDAAHTVGDGFSCRARVGLELLRDRHASFFSLHLLEYKELKGNGPFTIFVPHADLMTNLSQDELARIRAQRQLVFRYHVVGCRQLRSQELLEEGYVTTLSGHPLRFSEREGSIYINDYARVVSSDQQAVNGILHFIDRVLLPPDALHWEPDAAPIPRRNVTAAAESFGYKIFSSLVTVAGLLPLLQDGSHRPLTMLWPTDSALRALPRDQQAWLYHEDHRDKLAAILRGHVIRNVEALASDLPNLGPLRTMHGTPISFSCSRARPGELMVGEDDARIVQRHLPFEGGLAYGIDQLLEPPGLGARCDRFETRPLHLKICSICGLEPPCPQGSQEQGSPEACWRYYSKFWMSPPLYSLALRGIWPQPSLWGPPQGLGRGCHRNCVTTTWKPSCCPGHYGSECRACPGGASSPCSDHGVCMDGMSGSGQCRCRSGFAGTACELCAPGAFGPHCQACRCTSHGRCDEGLGGSGSCFCDEGWTGSRCEVQLELQPVCAPPCAPEAVCRVGNSCECSLGYEGDGHTCTVADLCQDGHGGCSEHANCSQVGTVVTCTCLPDYEGDGWSCRARDPCADGHRGGCSEHADCLNTGPNTRRCVCHAGYVGDGLQCLEEPEPPVDRCLGQPPPCHVDAVCTDLHFQEKRAGVFHLQATSGPYGLNFSEAEAACGAQGAVLASLPQLSAAQQLGFHLCLVGWLANGSAAHPVVFPAADCGDGQVGVVSLGTRGNLSERWDAYCYREQDVACQCRDGFVGDGTSVCNGKLLDVLATTANFSTFYGMLLGYANATPRGLDFLDFLDDELTYKTLFVPVNEGFVDNMTLSGPDLELHASNTTFLSTNASQGTMLPAHSGLSLVISDVGPDNSTWVPVAPGAVVVSRVIVWDIMAFNGIIHALASPLLAPPQPHAVVAPEAPSVAVGAGAVVATGALLGLVAGALYLRARGKATGFGFSAFQAEDDADDDFSPWQEGASPTLVSVPNPVFGSHDAFCEPFDDSLLEEDFPDTQRILAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M0W3</accession>
    <name>A0A8C4M0W3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phagophore assembly factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">PHAF1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PHAF1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4M0W3"/>
    <dbReference type="Ensembl" id="ENSEAST00005017195.2">
        <property type="protein sequence ID" value="ENSEASP00005015812.2"/>
        <property type="gene ID" value="ENSEASG00005010953.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153528"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005373">
        <property type="entry name" value="PHAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039156">
        <property type="entry name" value="PHAF1/BROMI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465:SF2">
        <property type="entry name" value="PHAGOSOME ASSEMBLY FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13465">
        <property type="entry name" value="UPF0183 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03676">
        <property type="entry name" value="PHAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005015812.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="423" mass="47056" checksum="3F1CA82B5A497F96" modified="2023-09-13" version="2">MLDLEVVPERSLGNEQWEFTLGMPLAQAVAILQKHCRIIKNVQVLYSEQSPLSHDLILNLTQDGIKLLFDAFNQRLKVIEVYDLTKVKLKYCGVHFNSQAIAPTIEQIDQSFGATHPGVYNSAEQLFHLNFRGLSFSFQLDSWTEAPKYEPNFAHGLASLQIPHGATVKRMYIYSGNSLQDTKAPGMPLSCFLGNVYAESVDVLRDGTGPSGLRLRLLAAGCGPGLLADAKMRVFERSVYFGDSCQDVLSMLGSPHKVFYKSEDKMKIHSPSPHKQVPSKCNDYFFNYFTLGVDILFDANTHKVKKFVLHTNYPGHYNFNIYHRCEFKIPLAIKKENADGQTETCTTYSKPRRPSSKYGLEMTEVSGVATNILPVLPLCIRTGPAVLHVYLAGCTPGDSSPLPHLPPGSGALSPLVIQIGRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M1Y2</accession>
    <name>A0A8C4M1Y2_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Neurofascin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">NFASC</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4M1Y2"/>
    <dbReference type="Ensembl" id="ENSEAST00005017620.2">
        <property type="protein sequence ID" value="ENSEASP00005016215.2"/>
        <property type="gene ID" value="ENSEASG00005007892.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157024"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098632">
        <property type="term" value="F:cell-cell adhesion mediator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd05731">
        <property type="entry name" value="Ig3_L1-CAM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05845">
        <property type="entry name" value="IgI_2_L1-CAM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05875">
        <property type="entry name" value="IgI_hNeurofascin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000057">
        <property type="entry name" value="neural cell adhesion molecule L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000363">
        <property type="entry name" value="neurofascin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000512">
        <property type="entry name" value="neurofascin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000574">
        <property type="entry name" value="neurofascin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001360">
        <property type="entry name" value="neurofascin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000005">
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            <begin position="1"/>
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            <begin position="25"/>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M1Y4</accession>
    <name>A0A8C4M1Y4_EQUAS</name>
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        <recommendedName>
            <fullName evidence="14">Ubiquinone biosynthesis monooxygenase COQ6, mitochondrial</fullName>
            <ecNumber evidence="14">1.14.15.45</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">2-methoxy-6-polyprenolphenol 4-hydroxylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Coenzyme Q10 monooxygenase 6</fullName>
            <ecNumber evidence="14">1.14.15.46</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 16" type="primary">COQ6</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">FAD-dependent monooxygenase required for two non-consecutive steps during ubiquinone biosynthesis. Required for the C5-ring hydroxylation during ubiquinone biosynthesis by catalyzing the hydroxylation of 4-hydroxy-3-(all-trans-decaprenyl)benzoic acid to 3,4-dihydroxy-5-(all-trans-decaprenyl)benzoic acid. Also acts downstream of COQ4, for the C1-hydroxylation during ubiquinone biosynthesis by catalyzing the hydroxylation of 2-methoxy-6-(all-trans-decaprenyl)phenol to 2-methoxy-6-(all-trans-decaprenyl)benzene-1,4-diol. The electrons required for the hydroxylation reaction are funneled indirectly to COQ6 from NADPH via a ferredoxin/ferredoxin reductase system composed of FDX2 and FDXR.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2-methoxy-6-(all-trans-decaprenyl)phenol + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = 2-methoxy-6-(all-trans-decaprenyl)benzene-1,4-diol + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:81295"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:50774"/>
            <dbReference type="ChEBI" id="CHEBI:64180"/>
            <dbReference type="EC" id="1.14.15.46"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>4-hydroxy-3-(all-trans-decaprenyl)benzoate + 2 reduced [2Fe-2S]-[ferredoxin] + O2 + 2 H(+) = 3,4-dihydroxy-5-(all-trans-decaprenyl)benzoate + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:81259"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:62793"/>
            <dbReference type="ChEBI" id="CHEBI:84503"/>
            <dbReference type="EC" id="1.14.15.45"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="14">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9. Interacts with COQ8B and COQ7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Mitochondrion inner membrane</location>
            <topology evidence="14">Peripheral membrane protein</topology>
            <orientation evidence="14">Matrix side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Cell projection</location>
        </subcellularLocation>
        <text evidence="14">Localizes to cell processes and Golgi apparatus in podocytes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the UbiH/COQ6 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.45" evidence="14"/>
    <dbReference type="EC" id="1.14.15.46" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4M1Y4"/>
    <dbReference type="Ensembl" id="ENSEAST00005018250.2">
        <property type="protein sequence ID" value="ENSEASP00005016794.1"/>
        <property type="gene ID" value="ENSEASG00005011638.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015152"/>
    <dbReference type="OMA" id="VKQMQVW"/>
    <dbReference type="UniPathway" id="UPA00232"/>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Ubi_Hdrlase_CS"/>
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        <property type="entry name" value="Ubi-OHases"/>
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    <dbReference type="PANTHER" id="PTHR43876">
        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
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        <property type="entry name" value="UBIQUINONE BIOSYNTHESIS MONOOXYGENASE COQ6, MITOCHONDRIAL"/>
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    </dbReference>
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        <property type="entry name" value="RNGMNOXGNASE"/>
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    <keyword evidence="5 14" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 14" id="KW-0831">Ubiquinone biosynthesis</keyword>
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        <location>
            <begin position="257"/>
            <end position="368"/>
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        <source>
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    </evidence>
    <sequence length="531" mass="57266" checksum="6979CF903257F071" modified="2022-01-19" version="1">MQPPILPAQGGKNRFLIGLFSSHVLVPVLRWRLKLQVIGGLCSGSASSGGPSGAPSAAQMGTMAARLAVSRWVAARAAARRGPLVSCRRWSGVATDTVYDVVVSGGGLVGAAMACALGYDVHFCDKKILLLEVGPKKVLEKLPETYSNRVSSISPGSATLLSSFGAWDHICNMRYRAFRRMQVWDACSEALIVFDKDNLDDMGYIVENDVIMHALTKQLEAVSDRVTVLYRSKAVGYTWPYSFPMADSSPWVQITLGDGSTLQTKLLIGADGHNSGVRQAAGIQNVSWNYDQSAVVATLHLSEATENNVAWQRFLPSGPIALLPLSDTLSSLVWSTSHEHAAELVSMDEEKFVDAINSAFWSDVNHTDFIDSAGTMLHYAIAFLKPTKVSARQLPPSVARVDAKSRALFPLGLGHAAEYVRPRLALIGDAAHRVHPLAGQGVNMGFGDISSLVHHLSTAAFNGKDLGSMSHLTGYETDRQRHNTALLAATDVLKRLYSTSATPLVLLRTWGLQATNAVSPLKEQIMAFASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M2N3</accession>
    <name>A0A8C4M2N3_EQUAS</name>
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        <recommendedName>
            <fullName evidence="5">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <topology evidence="1">Single-pass membrane protein</topology>
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        <property type="gene ID" value="ENSEASG00005014239.2"/>
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        <property type="component" value="Chromosome 8"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
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    <dbReference type="InterPro" id="IPR037958">
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    <dbReference type="InterPro" id="IPR050143">
        <property type="entry name" value="TRIM/RBCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24103">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13765">
        <property type="entry name" value="PRY"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SPRY"/>
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    <dbReference type="PRINTS" id="PR01407">
        <property type="entry name" value="BUTYPHLNCDUF"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="5">
        <location>
            <begin position="128"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="110"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="123"/>
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        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="321" mass="36906" checksum="CA4EADD84555A50E" modified="2023-09-13" version="2">MQKCNIKFAKYGNSHILQRSICSIIFLNFNIFDKFFLKCKPTSDSACSSSSPPSFLSLFYAPSVPLLLCPSHLLLLLHFYLSYTGSFLLPCLKKLNIRIIQNIKRKSKGFRRRNVTKTKRNRKDNERKHLEENTSQKHGGRLVIAVNVTLDAATAHPVLLLSEKGRRVTWQERCQDLPSSTQRFNSLPCVLGQLDISSGRWYWEVEVGEARSWDLGICRDNVTRKERVTMSPQNGFWAIRLYDGDYWALTSRVTLLTLQEKPLIVGIFLDYEAGDVSFYNMTDGSHIFTFPQNTFYGILRPLFRLWSSDSGSLTIVQVEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8C4M416_EQUAS</name>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
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                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
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                <person name="A."/>
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                <person name="M."/>
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                <person name="D.D."/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4M416"/>
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        <property type="gene ID" value="ENSEASG00005014341.2"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="term" value="C:ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
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        <property type="entry name" value="60S RIBOSOMAL PROTEIN L23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14108" checksum="EFAFACBE3285DBDA" modified="2022-01-19" version="1">MALKVKKEAPAPPKAESKAKALKAKKAVMKAQYPCKSTLRSNKLDHYALIKFPLNTESAMKKIDNNTTLCSLWMSRPTSTKQAVKKIYNVDMAKVNKIRPDGEKKTYVRLVPDYDALDVANKIGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M433</accession>
    <name>A0A8C4M433_EQUAS</name>
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        <submittedName>
            <fullName evidence="6">Cilia and flagella associated protein 65</fullName>
        </submittedName>
    </protein>
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        <name evidence="6" type="primary">CFAP65</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
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                <person name="S."/>
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                <person name="Liu"/>
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                <person name="J."/>
                <person name="Li"/>
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                <person name="Hou"/>
                <person name="M."/>
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                <person name="G."/>
                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="Yu"/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSEAST00005018611.2">
        <property type="protein sequence ID" value="ENSEASP00005017136.2"/>
        <property type="gene ID" value="ENSEASG00005011849.2"/>
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        <property type="component" value="Chromosome 19"/>
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        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097225">
        <property type="term" value="C:sperm midpiece"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007288">
        <property type="term" value="P:sperm axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052614">
        <property type="entry name" value="CFAP65"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056344">
        <property type="entry name" value="Ig_CFAP65-like_9th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056305">
        <property type="entry name" value="Ig_CFAP65_10th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057470">
        <property type="entry name" value="Ig_CFAP65_7th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057467">
        <property type="entry name" value="Ig_CFAP65_8th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46127">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46127:SF1">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24291">
        <property type="entry name" value="Ig_CFAP65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25249">
        <property type="entry name" value="Ig_CFAP65_7th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25248">
        <property type="entry name" value="Ig_CFAP65_8th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24816">
        <property type="entry name" value="Ig_CFAP65__9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49354">
        <property type="entry name" value="PapD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CFAP65 seventh Ig-like" evidence="5">
        <location>
            <begin position="311"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="CFAP65 eight Ig-like" evidence="4">
        <location>
            <begin position="416"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="CFAP65-like ninth Ig-like" evidence="3">
        <location>
            <begin position="532"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="domain" description="CFAP65 tenth Ig-like" evidence="2">
        <location>
            <begin position="718"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1071"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1157"/>
            <end position="1222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1341"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1165"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1185"/>
            <end position="1213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25248"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF25249"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1386" mass="156805" checksum="8266761FAD49BF38" modified="2023-09-13" version="2">VTWTSGTCTQVQHPRIRDIPLMPSHLYCLASAQHLAGELTISTLTTIPRAHMFMLTARERNDLTVLLLRPSFPLLDPPYDTRQTPSPPSCIEHILIWSEFGAVPGPKDPSTVPSCAWMKITQKATLLFWVTPNTCDVPPLKSTAMRLHFQPPTKQHLLPWSRLQIYRKEDCTCVPFWCAFCDTRVGGPHTIMHRLEHNNHPVPSSLVPQLFPAVSASEPSYRSLLLINRGSMLLTFNLAPKSSSDIFLRPSSGLVAPGAHQIFLVCTYPKGNAWKQHIFYLQFNFCPQYLKEVSMQSREEPLQLKLDTCRSVFFKPTWVGCSSTSLFTFRNPSRLPLEFEWRVSQQHRKMLAVQPARGLIQPNESLTLTWTFSPLEETKYLFQVGMWVWEAGLPPNTKPPATTHYMLRLVGMGITSSLSAKEKELDFGNMLVNSKQSRLLVLLNEGSCTLYYRLFLEQGSPEVVGDDPLALQLDRMEGSVPPRSQDTICLTACPKHRSQYSWTISYSPLSHRDNKVGEKQELCRVFLTAVYPLLSVLDACSMGSAEGITRKHLWRLFSLDTLNSYLERDPTPWELTYKVPTRHSTNQIPPVFTPLKLTFNFGAAPIEAPPSVVLLALKNSGVVPLDWAFLFPSDQQIDLELWAEQAEFDATELHQMRVQDNCIFSISPKAGSLSPGQEQIVEFKYSHLFIGTDRLPVLFKVSHGREILLNFIGVTVKLEQKYVHFTSTSHQFTPVPIGDTLPPRQIYELYNGGSVPVTYEIQTNILSQVQEKNFNHPIFCCLNPQGEIQPGTTARVLWIFSPIEAKTYSVDVPIHILGWNSAIICFQGVGYDPHVMGDTAPFHNISSWDDSSIPSRLMVPGQNVFLSQSHISLGNIPVQSKCSRLLFLNNVSKNETIAFVWQLRPLDFGEVSVSPMRGKVAPEESVPFVVTLKASVHASFYSVDLVCKVYQHELLKQYNKELQEWKDEKERQEVEFTITDKKVKRRAYCAACEPVRKYKTLPPIKNQQSLNRPASWNLKIPKEETSWPCPQPPSPGMLCLGLTARAHATDYFLANFFSDFPRHFLHRDLPKRKQPREESEPSEEESPDNWTHVSKQKKQLLVDCLTTIIRGLLEDKIFHEAVDQSLVEQIPYFCQFWSEQSARFMAKNSSLHLAPVLSPSSSSFEDSRSKEQEEDKLKPGKKEALEEEEEEEEETEEEEETGKEELEEEELEGNEEKLGWVGVGPTMQPMSQESLKWQWQQQLKVIVKEEQEREEKEAISRLPAFANLQEALLENMIQNILVEASRGEVVLTSRPRVIALPPLSLPRWGLCPLLLGSSPSEAGGWKNDIWCCSRGPRSPPPSLHPCCQGLRGAPAPEPGRRQGEGGGRSASSESIFAPSKRAPGQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M4Z1</accession>
    <name>A0A8C4M4Z1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Beta-galactoside alpha-2,6-sialyltransferase 1</fullName>
            <ecNumber evidence="16">2.4.3.1</ecNumber>
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        <alternativeName>
            <fullName evidence="20">CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1</fullName>
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        <alternativeName>
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        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Sialyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">ST6GAL1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a beta-D-galactoside + CMP-N-acetyl-beta-neuraminate = an N-acetyl-alpha-neuraminyl-(2-&gt;6)-beta-D-galactosyl derivative + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52104"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28034"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:136398"/>
            <dbReference type="EC" id="2.4.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.3.1" evidence="16"/>
    <dbReference type="RefSeq" id="XP_014698839.1">
        <property type="nucleotide sequence ID" value="XM_014843353.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014698841.1">
        <property type="nucleotide sequence ID" value="XM_014843355.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014698843.1">
        <property type="nucleotide sequence ID" value="XM_014843357.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044627201.1">
        <property type="nucleotide sequence ID" value="XM_044771266.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044627202.1">
        <property type="nucleotide sequence ID" value="XM_044771267.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070365349.1">
        <property type="nucleotide sequence ID" value="XM_070509248.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070365350.1">
        <property type="nucleotide sequence ID" value="XM_070509249.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070365351.1">
        <property type="nucleotide sequence ID" value="XM_070509250.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070365352.1">
        <property type="nucleotide sequence ID" value="XM_070509251.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070365353.1">
        <property type="nucleotide sequence ID" value="XM_070509252.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070365354.1">
        <property type="nucleotide sequence ID" value="XM_070509253.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070365355.1">
        <property type="nucleotide sequence ID" value="XM_070509254.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070365356.1">
        <property type="nucleotide sequence ID" value="XM_070509255.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070365357.1">
        <property type="nucleotide sequence ID" value="XM_070509256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M4Z1"/>
    <dbReference type="SMR" id="A0A8C4M4Z1"/>
    <dbReference type="Ensembl" id="ENSEAST00005022243.2">
        <property type="protein sequence ID" value="ENSEASP00005020484.1"/>
        <property type="gene ID" value="ENSEASG00005014107.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106832544"/>
    <dbReference type="KEGG" id="eai:106832544"/>
    <dbReference type="CTD" id="6480"/>
    <dbReference type="GeneTree" id="ENSGT00940000157053"/>
    <dbReference type="OMA" id="THPEQPF"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003835">
        <property type="term" value="F:beta-galactoside alpha-2,6-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006054">
        <property type="term" value="P:N-acetylneuraminate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097503">
        <property type="term" value="P:sialylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd23985">
        <property type="entry name" value="GT29_ST6GAL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000012">
        <property type="entry name" value="ST6 beta-galactoside alpha-2,6-sialyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012163">
        <property type="entry name" value="Sialyl_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46059">
        <property type="entry name" value="BETA-GALACTOSIDE ALPHA-2,6-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46059:SF2">
        <property type="entry name" value="BETA-GALACTOSIDE ALPHA-2,6-SIALYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005557">
        <property type="entry name" value="Sialyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="46"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="21">
        <location>
            <begin position="184"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00069321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00076526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00076676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00080062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR005557-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="406" mass="46561" checksum="56678EF038B9AEF7" modified="2022-01-19" version="1">MIHSSLKKKFSFCVLVFLLFAVICVWKEKKKGSYYESLKLQTKELQMPRSPEKRAIGSGSKFASSSSTQDPHRNTQGLSNPRSPAKAKPEGSFQVWNKDSSSKNLIPRLQKIWKNYLSMNKYKVSYKGPGPGVKFSADVLRCRLRDEVNVSMVEATDFPFNTSEWEGYLPMEDIRTKAGPWGKCAVVSSAGSLKSSQLGQEIDDHDAVMRFNGAPTASFQQDVGTKTTIRLMNSQLVTTEGRFLKDSLYNEGILIVWDPSVYHSDIPKWYKNPDYSFFDNYKSYRKLHPDQPFYILKPQMPWELWDIIQEISPEEIQPNPPSSGMLGIIIMMTLCDQVDIYEFLPSKRKTDVCYYYQKYFDTACTMGAYHPLLFEKNMVKHLNQGTDEDIYLFGKATLPGFRSIRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M836</accession>
    <name>A0A8C4M836_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">UDP-glucuronosyltransferase</fullName>
            <ecNumber evidence="13">2.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">UDP-glucuronosyltransferases catalyze phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase water solubility and enhance excretion. They are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor beta-D-glucuronoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132367"/>
            <dbReference type="ChEBI" id="CHEBI:132368"/>
            <dbReference type="EC" id="2.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Membrane</location>
            <topology evidence="13">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.17" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4M836"/>
    <dbReference type="Ensembl" id="ENSEAST00005023439.2">
        <property type="protein sequence ID" value="ENSEASP00005021601.2"/>
        <property type="gene ID" value="ENSEASG00005014770.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161263"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043541">
        <property type="term" value="C:UDP-N-acetylglucosamine transferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000094">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF24">
        <property type="entry name" value="UDP-GLUCURONOSYLTRANSFERASE 3A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="37"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="455"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005021601.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="490" mass="56155" checksum="62C70ED3406662DF" modified="2023-09-13" version="2">MNEKATKPEKHKSSKSKHSNKYKIIGTKRYCYHHANVNPCCMIFLTFLNICVFLGFKEEEKSYQAITWLPPEDYNKEFKKYFDFFLKEALYMELQGLQCSHLLKRNDIMDSLENENFDLLFVDAFDLCSPLVAEKLGKPFVSVLPTTFGYMDFGLPSPLSYVPVFNSLLSDRMGFWGRVKNFLMFFDFSVKQWQTHSTFDNIIKEHFPEGSRPVLSHLLKKAELWFPLLPNTVYVGGLMAKPVKAVPQEFENFIAKFGDSGFVLVALGSLISNYSSHELLKEMNSAFAHLPQGVIWRCKPSHWPKDISLAENVKIVDWLPQSDLLAHPRIRLFVTHGGLNSIMEAIQHGVPMVGIPLFGDQPENLFRIEAKKFGVSIQVKQIKAETLALKMKQVIEDKRYKSTAVAASIIRRSHPLTPSQRLVGWTNHILQTGGAAHLKPYSFQESWYQQYLLDVFLFLLVVTLGTMWLCGKLLGMVARWLCGARKLKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MAE2</accession>
    <name>A0A8C4MAE2_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="27">Glycine receptor alpha 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">GLRA3</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Postsynaptic cell membrane</location>
            <topology evidence="20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="24">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014697629.1">
        <property type="nucleotide sequence ID" value="XM_014842143.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MAE2"/>
    <dbReference type="SMR" id="A0A8C4MAE2"/>
    <dbReference type="Ensembl" id="ENSEAST00005026318.2">
        <property type="protein sequence ID" value="ENSEASP00005024265.1"/>
        <property type="gene ID" value="ENSEASG00005016461.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831746"/>
    <dbReference type="KEGG" id="eai:106831746"/>
    <dbReference type="CTD" id="8001"/>
    <dbReference type="GeneTree" id="ENSGT00940000158368"/>
    <dbReference type="OrthoDB" id="407674at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016935">
        <property type="term" value="C:glycine-gated chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016934">
        <property type="term" value="F:extracellularly glycine-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016594">
        <property type="term" value="F:glycine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022852">
        <property type="term" value="F:glycine-gated chloride ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd19009">
        <property type="entry name" value="LGIC_ECD_GlyR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19060">
        <property type="entry name" value="LGIC_TM_GlyR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000002">
        <property type="entry name" value="Glycine receptor alpha 1 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000003">
        <property type="entry name" value="Glycine receptor alpha 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008127">
        <property type="entry name" value="Glycine_rcpt_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008130">
        <property type="entry name" value="Glycine_rcpt_A3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01673">
        <property type="entry name" value="GLYRALPHA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01676">
        <property type="entry name" value="GLYRALPHA3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00236">
        <property type="entry name" value="NEUROTR_ION_CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14" id="KW-0868">Chloride</keyword>
    <keyword evidence="12" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="10 22" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="18 24" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 24" id="KW-0406">Ion transport</keyword>
    <keyword evidence="17" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="9 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 24" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 24" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034590188" evidence="24">
        <location>
            <begin position="34"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="255"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="318"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel ligand-binding" evidence="25">
        <location>
            <begin position="48"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Neurotransmitter-gated ion-channel transmembrane" evidence="26">
        <location>
            <begin position="260"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <begin position="235"/>
            <end position="240"/>
        </location>
        <ligand>
            <name>strychnine</name>
            <dbReference type="ChEBI" id="CHEBI:90700"/>
            <note>antagonist</note>
        </ligand>
    </feature>
    <feature type="site" description="Important for obstruction of the ion pore in the closed conformation" evidence="21">
        <location>
            <position position="294"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="171"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="22">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR608127-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR608127-52"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR608127-53"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU000687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF02931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF02932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005024265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="464" mass="53770" checksum="5A31F73B24E77C1C" modified="2022-01-19" version="1">MAHVRHFRTLVSGFYFWEAALLLSLVATKETDSARSRSAPMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVTCNIFINSFGSIAETTMDYRVNIFLRQKWNDPRLAYSEYPDDSLDLDPSMLDSIWKPDLFFANEKGANFHEVTTDNKLLRIFKNGNVLYSIRLTLTLSCPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQDEAPVQVAEGLTLPQFLLKEEKDLRYCTKHYNTGKFTCIEVRFHLERQMGYYLIQMYIPSLLIVILSWVSFWINMDAAPARVALGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEYAAVNFVSRQHKELLRFRRKRKNKTEAFALEKFYRFSDTDDEVRESRFSFTAYGMGPCLQAKDGMTPKGPNHPVQVMPKSPDEMRKVFIDRAKKIDTISRACFPLAFLIFNIFYWVIYKILRHEDIHQQQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MCK4</accession>
    <name>A0A8C4MCK4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger protein 770</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ZNF770</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MCK4"/>
    <dbReference type="Ensembl" id="ENSEAST00005026734.2">
        <property type="protein sequence ID" value="ENSEASP00005024649.2"/>
        <property type="gene ID" value="ENSEASG00005016754.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005061477.1">
        <property type="protein sequence ID" value="ENSEASP00005054552.1"/>
        <property type="gene ID" value="ENSEASG00005016754.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835153"/>
    <dbReference type="KEGG" id="eai:106835153"/>
    <dbReference type="CTD" id="54989"/>
    <dbReference type="GeneTree" id="ENSGT00940000161963"/>
    <dbReference type="OrthoDB" id="8113227at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002212">
        <property type="entry name" value="Zinc finger protein 672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000286">
        <property type="entry name" value="Zinc finger protein 770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000904">
        <property type="entry name" value="Zinc finger protein 770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000978">
        <property type="entry name" value="Zinc finger protein 770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001194">
        <property type="entry name" value="Zinc finger protein 770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001196">
        <property type="entry name" value="Zinc finger protein 770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001860">
        <property type="entry name" value="Zinc finger protein 770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002585">
        <property type="entry name" value="Zinc finger protein 770"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408:SF34">
        <property type="entry name" value="ZINC FINGER PROTEIN 672-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="81"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="482"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="510"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="640"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="668"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005024649.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="693" mass="80349" checksum="A20ACAF36E572F69" modified="2023-09-13" version="2">MMAENNLKMLKIQQCVVANKLPRNRPYICNICFKHFETPSKLARHYLIHTGQKPFECDVCHKTFRQLVHLERHQLTHNLPFKCSICQRHFKNLKTFVKHQQLHNETYQNDVKQVRRLLEAKQEKPLYGMYHTFSPEERWALHPCSKSDPAYNPTKKRKNIHACTICGKMFPSQSKLDRHALIHTGQRPFKCVLCSKSFRQSTHLKIHQLTHSEERPFQCCFCQKGFKIQSKLLKHKQIHTRNKTFQTLTLKVKSPESCPLPNKLNAKQDGFENGDIGESEENNQLDVHSIYIVPFQCPECEECFESEQIFNGHKCFPARGGKIPSRLKRSYNYKTIVKKILAKLKRAGGKKLDNFRSEKKVFKNSLLKNCDLISGEQSPEQTQRTFMGSLGKHGTYKTVGNKKKKTLTLPFSWQKHFQSQNMGKNLKGILSTENVLTMDNSVNNKDVSVYGSSGEEFCDNCEVLQCGFSLPSENIHAGHKMCPCDKCEKVFPSISKLQRHYLIHTGQRPFGCNVCGKSFRQSAHLKRHKLTHIDKIPYRRSLCQVEFENLNKLFIHPGDNVNYNASQQCQPLGFQKYEMSEPDQISEIKVKAESEDFILGTPFRNRQPYLSSALLESEQSHHSHCCSYPGRTERNDGLLYQCSVCSKSFRSPSKLERHYLIHAGQKPFECSVCGKTFRQAPHWKRHQLTHFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MJA8</accession>
    <name>A0A8C4MJA8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">GIT ArfGAP 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">GIT2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_014723252.1">
        <property type="nucleotide sequence ID" value="XM_014867766.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MJA8"/>
    <dbReference type="Ensembl" id="ENSEAST00005029761.2">
        <property type="protein sequence ID" value="ENSEASP00005027411.1"/>
        <property type="gene ID" value="ENSEASG00005018663.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106848071"/>
    <dbReference type="CTD" id="9815"/>
    <dbReference type="GeneTree" id="ENSGT00940000156383"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008277">
        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036465">
        <property type="term" value="P:synaptic vesicle recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd08847">
        <property type="entry name" value="ArfGap_GIT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000013">
        <property type="entry name" value="ARF GTPase-activating protein GIT1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000003">
        <property type="entry name" value="ARF GTPase-activating protein GIT2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.330:FF:000002">
        <property type="entry name" value="ARF GTPase-activating protein GIT2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.330">
        <property type="entry name" value="Nucleotidyltransferases domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
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    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
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    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047161">
        <property type="entry name" value="GIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022018">
        <property type="entry name" value="GIT1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013724">
        <property type="entry name" value="GIT_SHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46097:SF4">
        <property type="entry name" value="ARF GTPASE-ACTIVATING PROTEIN GIT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46097">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE INTERACTING ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12205">
        <property type="entry name" value="GIT1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08518">
        <property type="entry name" value="GIT_SHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00555">
        <property type="entry name" value="GIT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="10">
        <location>
            <begin position="1"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="7">
        <location>
            <begin position="166"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="377"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="433"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="504"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="385"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="433"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="505"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="520"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005027411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="709" mass="79040" checksum="86E519E27D797E3A" modified="2022-01-19" version="1">MSKRLRSSEVCADCSGPDPSWASVNRGTFICDECCSVHRSLGRHISQVRHLKHTPWPPTLLQMVETLYNNGANSIWEHSLLDPASIMSGRRKANPQDKVHPNKAEFIRAKYQMLAFVHRLPCRDDDSVTAKDLSKQLHSSVRTGNLETCLRLLSLGAQANFFHPEKGNTPLHVASKAGQILQAELLAVYGADPGTQDSSGKTPVDYARQGGHHELAERLVEIQYELTDRLAFYLCGRKPDHKNGQHFIIPQMADSSLDLSELAKAAKKKLQSLSNHLFEELAMDVYDEVDRRETDAVWLATQNHSTLVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSPLSSSKDNVELILKTVSNQHSVESQDNDQPDYDSVASDEDTDLEATASKANRQKLQTLQSENSNLRKQATTNIYQVQTGSEYTDTSNHSSLKRRPSARGSRPMSMYETGSGQKPYLPMGEVNHPEESRTRLQPFPAHIGRSALVTSSSSLPSFPSTLSWSRDESTRRTSRLEKQNSTPESDYDNTPNDTDPDDMGSSRKGRQRSMVWQGDGSVPDTAEAHTAPSPVLPSTEDVIRKTEQITKNIQELLRAAQENKHDSYIPCSERIHVAVTEMAALFPKKPKSDMVRTSLRLLTSSAYRLQSECKKTLPGDSSPPTDIQLVTQQVIQCAYDIAKAAKQLVTITTKENNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MK76</accession>
    <name>A0A8C4MK76_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thiamine transporter 1</fullName>
            <shortName evidence="3">ThTr-1</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Solute carrier family 19 member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SLC19A2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">High-affinity transporter for the intake of thiamine.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the reduced folate carrier (RFC) transporter (TC 2.A.48) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014700629.2">
        <property type="nucleotide sequence ID" value="XM_014845143.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MK76"/>
    <dbReference type="Ensembl" id="ENSEAST00005030422.2">
        <property type="protein sequence ID" value="ENSEASP00005028011.2"/>
        <property type="gene ID" value="ENSEASG00005019041.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106833799"/>
    <dbReference type="KEGG" id="eai:106833799"/>
    <dbReference type="CTD" id="10560"/>
    <dbReference type="GeneTree" id="ENSGT00950000183022"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015234">
        <property type="term" value="F:thiamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031923">
        <property type="term" value="P:pyridoxine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002666">
        <property type="entry name" value="Folate_carrier"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028338">
        <property type="entry name" value="ThTr-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10686">
        <property type="entry name" value="FOLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10686:SF19">
        <property type="entry name" value="THIAMINE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01770">
        <property type="entry name" value="Folate_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028739">
        <property type="entry name" value="Folate_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500794">
        <property type="entry name" value="Thiamine_transporter_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
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            <begin position="127"/>
            <end position="147"/>
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    <feature type="transmembrane region" description="Helical" evidence="4">
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            <begin position="159"/>
            <end position="180"/>
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    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="186"/>
            <end position="206"/>
        </location>
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            <begin position="293"/>
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        </location>
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            <begin position="330"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="359"/>
            <end position="377"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
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            <begin position="383"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="419"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR028739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005028011.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="495" mass="55119" checksum="89AD971E9EC9DC6C" modified="2023-09-13" version="2">MEVPGPVSRRAAAATMLLRTARVPRECWFLPTALLCAYGFFASLRPSEPFLTPYLLGPDKNLTEREVFNEIYPVWTYSYLVLLFPVFLATDYLRYKPVVLLQGLSLIVTWFMLLYAQGLLAVQFLEFFYGVATATEIAYYSYIYSVVDLSMYQKVTSYCRSATLVGFTVGSVLGQILVSVAGWSLFSLNVISLTCVSVAFAVAWFLPMPQKSLFFHHVPSACQGMNGIKVQNGGMVTDTSASNHLPGWEDIESKIPLNVEERPMEEPEPKPDHLLVLRVLWNDFSVCYSSRPLLCWSVWWALSTCGYFQVVNYTQGLWEVVMPSRDAAIYNGGVEAVSTLLGAVAVFAVGYIKISWSTWGEIALSLFSLLIAATVYIMDTAGTIWVCYASYVVFRIIYMLLITIATFQIAANLSMERYALVFGVNTFIALALQTLLTLIVVDASGLGLEITTQFLIYAGYFALIAVVFLVNGAVSVMKKYRKQEDPESSSQVTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MLG2</accession>
    <name>A0A8C4MLG2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Complement component C8 gamma chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">C8G</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis. The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways. The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. C8G, together with C8A and C8B, inserts into the target membrane, but does not form pores by itself. During MAC assembly, associates with C5b, C6 and C7 to form the C5b8 intermediate complex that inserts into the target membrane and traverses the bilayer increasing membrane rigidity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Heterotrimer of 3 chains: alpha (C8A), beta (C8B) and gamma (C8G); the alpha and gamma chains are disulfide bonded. Component of the membrane attack complex (MAC), composed of complement C5b, C6, C7, C8A, C8B, C8G and multiple copies of the pore-forming subunit C9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MLG2"/>
    <dbReference type="Ensembl" id="ENSEAST00005027818.2">
        <property type="protein sequence ID" value="ENSEASP00005025620.2"/>
        <property type="gene ID" value="ENSEASG00005017470.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000034309"/>
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        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072562">
        <property type="term" value="C:blood microparticle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005579">
        <property type="term" value="C:membrane attack complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001848">
        <property type="term" value="F:complement binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019841">
        <property type="term" value="F:retinol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006957">
        <property type="term" value="P:complement activation, alternative pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006958">
        <property type="term" value="P:complement activation, classical pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000015">
        <property type="entry name" value="Complement component C8 gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002968">
        <property type="entry name" value="A1-microglobln"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043245">
        <property type="entry name" value="C8G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022272">
        <property type="entry name" value="Lipocalin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47304">
        <property type="entry name" value="COMPLEMENT COMPONENT C8 GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47304:SF1">
        <property type="entry name" value="COMPLEMENT COMPONENT C8 GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01215">
        <property type="entry name" value="A1MCGLOBULIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00213">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0179">Complement alternate pathway</keyword>
    <keyword evidence="9" id="KW-0180">Complement pathway</keyword>
    <keyword evidence="7" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0683">Retinol-binding</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="18">
        <location>
            <begin position="151"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00093391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025620.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="305" mass="32969" checksum="8A34ED2B59B2D3C9" modified="2023-09-13" version="2">MGGAAGQAEGCGLRPGAGWALRHGSRALPRSAGSRSDVQDEAEFMRSLAGAGDPGPGRRFEEGGGLTWTSWPGFLGSTGQWTLTWELAIPPSASPSPVMVTAAAMLPPRTVLLTLLLAAVSLGQRAQRPPRPLSPISTIQPKANFDAQQFAGTWLLVAVASSCRFLQEQGHRAEATALHLAPQGTAMIASTFQKLDGICWQVRQLYRDMGGPGRFQLQARGARGAIDMVVGETDYRSFAILYLERARRLSVKLYTRSLPVSDTALSVFEQRVQGANLTEDHILFFPKYGFCEAADQFHVLDEVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MNK2</accession>
    <name>A0A8C4MNK2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dorsal root ganglia homeobox protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Paired-related homeobox protein-like 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">DRGX</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Transcription factor required for the formation of correct projections from nociceptive sensory neurons to the dorsal horn of the spinal cord and normal perception of pain.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with RGMB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014697057.2">
        <property type="nucleotide sequence ID" value="XM_014841571.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MNK2"/>
    <dbReference type="Ensembl" id="ENSEAST00005030988.2">
        <property type="protein sequence ID" value="ENSEASP00005028525.2"/>
        <property type="gene ID" value="ENSEASG00005019407.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831402"/>
    <dbReference type="KEGG" id="eai:106831402"/>
    <dbReference type="CTD" id="644168"/>
    <dbReference type="GeneTree" id="ENSGT00940000159008"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009593">
        <property type="term" value="P:detection of chemical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016048">
        <property type="term" value="P:detection of temperature stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021516">
        <property type="term" value="P:dorsal spinal cord development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050954">
        <property type="term" value="P:sensory perception of mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021559">
        <property type="term" value="P:trigeminal nerve development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000126">
        <property type="entry name" value="dorsal root ganglia homeobox protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050649">
        <property type="entry name" value="Paired_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329:SF542">
        <property type="entry name" value="DORSAL ROOT GANGLIA HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 12 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 12 13" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="15">
        <location>
            <begin position="31"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="16">
        <location>
            <begin position="204"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="12">
        <location>
            <begin position="33"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="88"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="220"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="89"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="110"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="251"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00078248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005028525.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="263" mass="28625" checksum="A957DDE6907905B0" modified="2023-09-13" version="2">MFYFHCPPQLEGTAPFGNHSTGDFDDGFLRRKQRRNRTTFTLQQLEALEAVFAQTHYPDVFTREELAMKINLTEARVQVWFQNRRAKWRKTERGASEQEPGAKEPMAEVTPPPVRNINSPPPGDQARSKKEALEAQQSLGRTVGPTGPFFPSCLPGTLLNTATYAQALSHVASLKGGPLCSCCVPDTMGLSFLPTYGCQSNRTASVAALRMKAREHSEAVLQSANLLGSSSSSPSPAAKPAPPDGSQDKTPPTKDHSEGEKSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MNL3</accession>
    <name>A0A8C4MNL3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 20">Protein kinase C</fullName>
            <ecNumber evidence="4 20">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="28" type="primary">PRKCQ</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="4 20"/>
    <dbReference type="RefSeq" id="XP_014696809.1">
        <property type="nucleotide sequence ID" value="XM_014841323.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070356975.1">
        <property type="nucleotide sequence ID" value="XM_070500874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MNL3"/>
    <dbReference type="Ensembl" id="ENSEAST00005030707.2">
        <property type="protein sequence ID" value="ENSEASP00005028276.1"/>
        <property type="gene ID" value="ENSEASG00005019221.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831239"/>
    <dbReference type="KEGG" id="eai:106831239"/>
    <dbReference type="CTD" id="5588"/>
    <dbReference type="GeneTree" id="ENSGT00940000157638"/>
    <dbReference type="OMA" id="EIWIELK"/>
    <dbReference type="OrthoDB" id="63267at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016235">
        <property type="term" value="C:aggresome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001772">
        <property type="term" value="C:immunological synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035739">
        <property type="term" value="P:CD4-positive, alpha-beta T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006509">
        <property type="term" value="P:membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046627">
        <property type="term" value="P:negative regulation of insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070233">
        <property type="term" value="P:negative regulation of T cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000563">
        <property type="term" value="P:positive regulation of CD4-positive, alpha-beta T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032740">
        <property type="term" value="P:positive regulation of interleukin-17 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032743">
        <property type="term" value="P:positive regulation of interleukin-2 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032753">
        <property type="term" value="P:positive regulation of interleukin-4 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000318">
        <property type="term" value="P:positive regulation of T-helper 17 type immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000570">
        <property type="term" value="P:positive regulation of T-helper 2 cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0032206">
        <property type="term" value="P:positive regulation of telomere maintenance"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0090330">
        <property type="term" value="P:regulation of platelet aggregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="CDD" id="cd20834">
        <property type="entry name" value="C1_nPKC_theta-like_rpt1"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd20837">
        <property type="entry name" value="C1_nPKC_theta-like_rpt2"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd05619">
        <property type="entry name" value="STKc_nPKC_theta"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.60.20:FF:000003">
        <property type="entry name" value="Protein kinase C delta"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.60.40.150:FF:000049">
        <property type="entry name" value="Protein kinase C delta type"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.60.20:FF:000008">
        <property type="entry name" value="Protein kinase C theta"/>
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        <property type="entry name" value="Protein kinase C, theta"/>
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        <property type="match status" value="2"/>
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    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
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    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
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    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
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    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR034668">
        <property type="entry name" value="nPKC_theta"/>
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    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014376">
        <property type="entry name" value="Prot_kin_PKC_delta"/>
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    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21494">
        <property type="entry name" value="PKC_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000551">
        <property type="entry name" value="PKC_delta"/>
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    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
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    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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        <property type="entry name" value="AGC_KINASE_CTER"/>
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        <property type="entry name" value="ZF_DAG_PE_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 20 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 20" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="26">
        <location>
            <begin position="159"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="26">
        <location>
            <begin position="231"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="380"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="27">
        <location>
            <begin position="635"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="21">
        <location>
            <position position="504"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <begin position="386"/>
            <end position="394"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22 23">
        <location>
            <position position="409"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022527"/>
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    <evidence type="ECO:0000256" key="23">
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MQ66</accession>
    <name>A0A8C4MQ66_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Inositol oxygenase</fullName>
            <ecNumber evidence="4 15">1.13.99.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 15">Myo-inositol oxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">MIOX</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 15">
            <text>myo-inositol + O2 = D-glucuronate + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23696"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
            <dbReference type="ChEBI" id="CHEBI:58720"/>
            <dbReference type="EC" id="1.13.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13 15">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="13 15">Binds 2 iron ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Polyol metabolism; myo-inositol degradation into D-glucuronate; D-glucuronate from myo-inositol: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the myo-inositol oxygenase family.</text>
    </comment>
    <dbReference type="EC" id="1.13.99.1" evidence="4 15"/>
    <dbReference type="RefSeq" id="XP_014720021.1">
        <property type="nucleotide sequence ID" value="XM_014864535.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MQ66"/>
    <dbReference type="SMR" id="A0A8C4MQ66"/>
    <dbReference type="Ensembl" id="ENSEAST00005032886.2">
        <property type="protein sequence ID" value="ENSEASP00005030247.2"/>
        <property type="gene ID" value="ENSEASG00005020592.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106846012"/>
    <dbReference type="KEGG" id="eai:106846012"/>
    <dbReference type="CTD" id="55586"/>
    <dbReference type="GeneTree" id="ENSGT00390000016211"/>
    <dbReference type="OrthoDB" id="5151075at2759"/>
    <dbReference type="UniPathway" id="UPA00111">
        <property type="reaction ID" value="UER00527"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008106">
        <property type="term" value="F:alcohol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050113">
        <property type="term" value="F:inositol oxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019310">
        <property type="term" value="P:inositol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007828">
        <property type="entry name" value="Inositol_oxygenase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12588:SF0">
        <property type="entry name" value="INOSITOL OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12588">
        <property type="entry name" value="MYOINOSITOL OXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05153">
        <property type="entry name" value="MIOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13 15" id="KW-0408">Iron</keyword>
    <keyword evidence="7 13 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="12">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="85"/>
            <end position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="141"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="220"/>
            <end position="221"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="253"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="14">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR607828-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR607828-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR607828-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030247.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="285" mass="33110" checksum="52A5ACB95DD5C06D" modified="2023-09-13" version="2">MKVIVGPDPSLVYRPDVDPETVKDKSSFRNYTSGPLLDRVFATYKLMHTHQTVDFVRKKHSQFGGFSYKKMTVLEAVDMLDRLVDESDPDVDFPNSFHAFQTAEGIRKAHPDKDWFHLVGLLHDLGKVLVLAGEPQWAVVGDTFPVGCRPQASVVFRDSTFQDNPDLQDPRYSTELGMYQPHCGLKNVLMSWGHDEYMYQMMKFNKFSLPLEAFYMIRFHSFYPWHTGGDYQQLCSEQDLAMLPWVQEFNKFDLYTKCPDLPDVDKLRPYYQGLIDKYCPGMLSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MRG0</accession>
    <name>A0A8C4MRG0_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Sucrase-isomaltase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">SI</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070365655.1">
        <property type="nucleotide sequence ID" value="XM_070509554.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005032085.2">
        <property type="protein sequence ID" value="ENSEASP00005029525.2"/>
        <property type="gene ID" value="ENSEASG00005020052.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106842405"/>
    <dbReference type="CTD" id="6476"/>
    <dbReference type="GeneTree" id="ENSGT00940000161633"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004558">
        <property type="term" value="F:alpha-1,4-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004574">
        <property type="term" value="F:oligo-1,6-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06602">
        <property type="entry name" value="GH31_MGAM_SI_GAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd14752">
        <property type="entry name" value="GH31_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00111">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000001">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000005">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1760:FF:000001">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000016">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.110.10:FF:000003">
        <property type="entry name" value="Maltase-glucoamylase, intestinal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1760">
        <property type="entry name" value="glycosyl hydrolase (family 31)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.110.10">
        <property type="entry name" value="Spasmolytic Protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030458">
        <property type="entry name" value="Glyco_hydro_31_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048395">
        <property type="entry name" value="Glyco_hydro_31_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030459">
        <property type="entry name" value="Glyco_hydro_31_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025887">
        <property type="entry name" value="Glyco_hydro_31_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000322">
        <property type="entry name" value="Glyco_hydro_31_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017957">
        <property type="entry name" value="P_trefoil_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000519">
        <property type="entry name" value="P_trefoil_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044913">
        <property type="entry name" value="P_trefoil_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762">
        <property type="entry name" value="ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22762:SF66">
        <property type="entry name" value="SUCRASE-ISOMALTASE, INTESTINAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13802">
        <property type="entry name" value="Gal_mutarotas_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01055">
        <property type="entry name" value="Glyco_hydro_31_2nd"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21365">
        <property type="entry name" value="Glyco_hydro_31_3rd"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00088">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00018">
        <property type="entry name" value="PD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57492">
        <property type="entry name" value="Trefoil"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00129">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00707">
        <property type="entry name" value="GLYCOSYL_HYDROL_F31_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00025">
        <property type="entry name" value="P_TREFOIL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51448">
        <property type="entry name" value="P_TREFOIL_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0765">Sulfation</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="domain" description="P-type" evidence="17">
        <location>
            <begin position="58"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="P-type" evidence="17">
        <location>
            <begin position="924"/>
            <end position="975"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005029525.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1824" mass="210013" checksum="AB24C1028C2E04A9" modified="2023-09-13" version="2">MAKKKFTPLEIILIVLFVIVTIIAIALIIVLATKTPAVEGVSTSTPATSTTTAYPGSENCPSELNDAINERINCIPEQFPTQALCASRGCCWRPWNDSVIPWCFFVDNHGYNVEEMTTNNAGLEARLNRIPSPTLYGDDINSVLLTTQSQTPNRFRFKITDPNNRRYEVPHQFVKEPTGTTDSETLYDVQVTENPFSIKVIRKSNNRTLFDTSIGPLVYSDQYLQISTRLPSEYIYGIGEHIHKRFRHDLYWKKWPLFTRDQLPGDNNNNLYGHQTFFMCIEDTSGKSFGVFLMNSNAMEIFIQPTPIVTYRVIGGILDFYIFLGDTPEQVVQQYQELIGLPAMPSYWSLGFQLSRWNYKSLDVVKEVVRRNREAGIPFDTQVTDIDYMEDKKDFTYDKVTFSGLPEFVQDLHDHGQKYVIILDPAISIDRRADGTAYEAYERGNAQKVWVNESDGTTAIIGEVWPGLTVYPDFTNPSCIDWWANECSIFHQEVPYDGIWIDMNEVSSFVQGSLKGCDVNKLNYPPFTPDILDKLLYSKTICMDAVQYWGKQYDVHSLYGYSMAIATEQAVQKVFPNKRSFILTRSTFAGSGSYAAHWLGDNTASWEQMEWSIAGMLEFGLFGMPLVGADICGFVADTTEELCRRWMQLGAFYPFSRNHNADGYVEQDPAFFGQDSLLVRSSKYYLNIRYSLLPFLYTLFYKAHKFGETVARPILHEFYEDTNSWIEDTQFLWGPALLITPVLKEGADTVSAYIPDATWYDYETGAKRPWRKQRVNMYLPADKIGLHLRGGYIIPTQEPAVTTNASRQNPLGLIVPLDENNTAKGDFFWDDGETKDTIENGNYILYTFSVSDNKLGIICTHSSYQEGTTLAFETIKILGLTETVTYVLVGEENQPTQAHSNFTYYPSNQSLLIYNLNFNLGRNFTVQWDQSFPENEKFTCYPDADIATEEKCRQRGCLWEPSSFGSRAPDCYFPREDNPYLVSSIQYSSMGVTADLQLNTAKAQINLPSEPVSTLRVEVKYHKNDMLQFKIYDAQNKRYEVPVPLNIPDTPTSTYENRLYDVEIKENPFGIQIRRRSTGTVIWDSQLPGFAFNDQFIQISTRLPSEYIYGFGEVEHTAFKRDLNWHTWGMFTRDQPPGYKLNSYGFHPYYMALEDESNAHGVFLLNSNGMDVTFQPTPALTYRIIGGILDFYMFLGPHPEVATKQYHEVIGQPVMPPYWSLGFQLCRYGYRNTSQVQQVYEEMVAARIPYDVQYTDINYMERQLDFTIGEAFSDLPQFVDRIRQEGMRYIIILDPAISGNETQPYPAFERGQEKDVFVKWPNTDEICWAKVWPDLPNITIDESLTEDEAVNASRAHAAFPDFFRNSTAQWWAKEILDFYNNKMKFDGLWIDMNEPSSFVNGTTTNQCRNEGLNYPPYFPELTKRTDGLHFRTLCMETEQILSDGSSVLHYDVHNLYGWSQVKPTYDALQRTTGKRGIVISRSTYPTAGRWGGHWLGDNYARWDNMDKSIIGMMEFSLFGISYTGADICGFFNDTEYQLCARWMQLGAFYPYSRNHNIANTRRQDPASWNETFAAMSRDILNVRYTLLPYFYTQLHEVHVQGGTVIRPLLHEFFNEKPTWDIFKQFLWGPAFMVTPVMEPNVDVVQGYVPNARWFDYHTGEDIGFRGNFHVFDAPLDKINLHVRGGHILPCQEPAQNTFYSRQNYMRLIVAADDNHTAQGSLFWDDGDTINTYERDLYFLIQFNYNHTTLTSTVLKNGYINRSEMRLGFINIWGKGKTPVQQVHLTYDENTYSLQFTQEADKEILNIDLRPNNFTLDEPIEIKWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MRL2</accession>
    <name>A0A8C4MRL2_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Major histocompatibility complex, class II, DO alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HLA-DOA</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the MHC class II family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MRL2"/>
    <dbReference type="Ensembl" id="ENSEAST00005033170.2">
        <property type="protein sequence ID" value="ENSEASP00005030512.2"/>
        <property type="gene ID" value="ENSEASG00005020773.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162112"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042613">
        <property type="term" value="C:MHC class II protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023026">
        <property type="term" value="F:MHC class II protein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019886">
        <property type="term" value="P:antigen processing and presentation of exogenous peptide antigen via MHC class II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002587">
        <property type="term" value="P:negative regulation of antigen processing and presentation of peptide antigen via MHC class II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045580">
        <property type="term" value="P:regulation of T cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd21004">
        <property type="entry name" value="IgC1_MHC_II_alpha_HLA_DO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000280">
        <property type="entry name" value="HLA class II histocompatibility antigen, DR alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.320.10">
        <property type="entry name" value="Class II Histocompatibility Antigen, M Beta Chain, Chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050160">
        <property type="entry name" value="MHC/Immunoglobulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014745">
        <property type="entry name" value="MHC_II_a/b_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001003">
        <property type="entry name" value="MHC_II_a_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944:SF44">
        <property type="entry name" value="HLA CLASS II HISTOCOMPATIBILITY ANTIGEN, DO ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19944">
        <property type="entry name" value="MHC CLASS II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00993">
        <property type="entry name" value="MHC_II_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00920">
        <property type="entry name" value="MHC_II_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0491">MHC II</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="214"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="13">
        <location>
            <begin position="105"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030512.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="254" mass="28384" checksum="C79076691BB7B331" modified="2023-09-13" version="2">MLEIDTVSSLSLALNSPNAADHMASYGPAFYQSYGASGQFSHEFDGEQLFSVELKKREAAWRLPEFGDLTRFDPQNGLASIAMIRVHLDVLVERSNRTRAINVPPRVTILPKSRVELGQPNVLICIVENIFPPVINITWLRNGQTITEGVAQTSFYSQPDHMFRKFHYLTFVPSADDFYDCKVEHWGLAEPLLRHWEPQVPILPPDITETLICVLGLAIGLVSFLVGTILLIISTCLSGAPRRRGPGRLGKESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MTE5</accession>
    <name>A0A8C4MTE5_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Elongation factor for RNA polymerase II 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ELL2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2 7">Belongs to the ELL/occludin family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A8C4MTE5"/>
    <dbReference type="Ensembl" id="ENSEAST00005031052.2">
        <property type="protein sequence ID" value="ENSEASP00005028584.2"/>
        <property type="gene ID" value="ENSEASG00005019439.2"/>
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    <dbReference type="GeneTree" id="ENSGT00940000154828"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 11"/>
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        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:snRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="RNA polymerase II elongation factor ELL2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2670">
        <property type="entry name" value="E3 ubiquitin-protein ligase"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR042065">
        <property type="entry name" value="E3_ELL-like"/>
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    <dbReference type="InterPro" id="IPR031176">
        <property type="entry name" value="ELL/occludin"/>
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    <dbReference type="InterPro" id="IPR019464">
        <property type="entry name" value="ELL_N"/>
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    <dbReference type="InterPro" id="IPR010844">
        <property type="entry name" value="Occludin_ELL"/>
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    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
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    <dbReference type="PANTHER" id="PTHR23288">
        <property type="entry name" value="OCCLUDIN AND RNA POLYMERASE II ELONGATION FACTOR ELL"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR23288:SF8">
        <property type="entry name" value="RNA POLYMERASE II ELONGATION FACTOR ELL2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ELL"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF07303">
        <property type="entry name" value="Occludin_ELL"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="occludin/ELL-like"/>
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    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51980">
        <property type="entry name" value="OCEL"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="OCEL" evidence="9">
        <location>
            <begin position="527"/>
            <end position="637"/>
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    <feature type="region of interest" description="Disordered" evidence="8">
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            <begin position="170"/>
            <end position="204"/>
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    <feature type="region of interest" description="Disordered" evidence="8">
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    <feature type="region of interest" description="Disordered" evidence="8">
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            <begin position="498"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="8">
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            <begin position="184"/>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MTN5</accession>
    <name>A0A8C4MTN5_EQUAS</name>
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        <recommendedName>
            <fullName evidence="12">UDP-glucuronosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.17</ecNumber>
        </recommendedName>
    </protein>
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        <name evidence="13" type="primary">LOC106840160</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor beta-D-glucuronoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132367"/>
            <dbReference type="ChEBI" id="CHEBI:132368"/>
            <dbReference type="EC" id="2.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Membrane</location>
            <topology evidence="12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.17" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4MTN5"/>
    <dbReference type="Ensembl" id="ENSEAST00005029557.2">
        <property type="protein sequence ID" value="ENSEASP00005027224.2"/>
        <property type="gene ID" value="ENSEASG00005025596.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153212"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000001">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000081">
        <property type="entry name" value="UDP-glucuronosyltransferase 2A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF12">
        <property type="entry name" value="UDP-GLUCURONOSYLTRANSFERASE 2B4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040536689" description="UDP-glucuronosyltransferase" evidence="12">
        <location>
            <begin position="24"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="493"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005027224.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="528" mass="60946" checksum="5FB4DAABCEA1F445" modified="2023-09-13" version="2">MSLKWIPLLLLLQLSCYFSPGSAGKVLVWPTEYSHWINMKTILDELVQRGHEVTVLTASASILVDPNKPSAIKFEIYPASLTKHDYEVIFKTLVDTWTRLPKDSFWTHFTLMQEIIWQFFDNVLKLCNDAVLNKKLIRKLQESKFDIIFADAVGLCSELLAELLQIPLVYSLRFSPGHTIEKYGGGLPFPPSYVPVVMSELSDQMTFMERVKNMIYVIYFDFWFQTFNEKKWDQFYSEVLGRATKLYELRRKAEMWLVRTYWDFEFPRPFLPHFQFIGGYHCKPAKSLPKEMEEFAQSSGENGIVVFTLGSMVSNMTEERANVIASAFAQIPQKVIWRYDGKKPDTLGPNTRLYKWIPQNDLLGHPKTKAFITHGGANGIYEAIYHGIPVVGIPLFADQPDNVAHMKTKGAAVRLDFDRMTSSDLLNALKTVIHDPSYKDNAMKLSRIQHDQPMKPLDRAVFWIEFVMRHKGAKHLRPASHDLTWFQYHSLDVIGFLLACVATTVFVIIKCLLCCWKFAKTGKKEKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MTS2</accession>
    <name>A0A8C4MTS2_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Serpin family A member 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SERPINA11</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MTS2"/>
    <dbReference type="Ensembl" id="ENSEAST00005034274.2">
        <property type="protein sequence ID" value="ENSEASP00005031532.2"/>
        <property type="gene ID" value="ENSEASG00005021471.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162010"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.39.10:FF:000003">
        <property type="entry name" value="alpha-1-antitrypsin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.497.10:FF:000001">
        <property type="entry name" value="Serine protease inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.310.10:FF:000001">
        <property type="entry name" value="Serpin family A member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.310.10">
        <property type="entry name" value="Serpins superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF154">
        <property type="entry name" value="SERPIN A11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040244876" evidence="5">
        <location>
            <begin position="20"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="6">
        <location>
            <begin position="59"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005031532.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="422" mass="46987" checksum="B71C32AD00A7E2D3" modified="2023-09-13" version="2">MGPPWLWLLETGILASVCCQPFPAHGDKSLGVPQAPRDQLSEPAPAYHKVTPAITHFALRLYKQLAAETPGNIFFSPVSISSTLALLSLGAQGDTPTQILEGLGFNLTETPEADIHRGFQSLIHTLNRPSPKHELKVGNSLFLDRQLKPRQHFLDAIRERYGAFAFSANFTDSATTGRQINDYVRKQTYGQVVDCLQEFTRDTLMVLVNYVFLKAKWKHPFNRYQTQKQESFSVDERTSLRIPMMHQKEMHRFLYDQDVACTVLQIEYSGNAQALLILPDPGKMEQVEVALQPETLRKWGQLLLPSLLDLHLPRFSISGSYNLEEILPHIGLTNIFNLEADLSGITGQLNKTISRVSHKAAVDMSERGTEAGAASGLLAQPQSLNATSAPHAHFNRPFLVLLWEVTTQSLLFLGKVVNPAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MV04</accession>
    <name>A0A8C4MV04_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MV04"/>
    <dbReference type="Ensembl" id="ENSEAST00005034205.2">
        <property type="protein sequence ID" value="ENSEASP00005031468.2"/>
        <property type="gene ID" value="ENSEASG00005021425.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000039859"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002352">
        <property type="entry name" value="Eosinophil_major_basic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050976">
        <property type="entry name" value="Snaclec"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22991">
        <property type="entry name" value="PROTEIN CBG13490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22991:SF40">
        <property type="entry name" value="PROTEIN CBG13490"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00770">
        <property type="entry name" value="EMAJORBASICP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040402506" description="C-type lectin domain-containing protein" evidence="3">
        <location>
            <begin position="21"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="4">
        <location>
            <begin position="177"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005031468.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="242" mass="26698" checksum="238AD44CF2F81F37" modified="2023-09-13" version="2">MKLPLLLALLFGTCEGGAFGLSLRRSETSNFENPLGDDTLPQDGEMPERGAMEAPMEGLTLLEGEEEGDSGSEVVPEEEALIKSVSVLEEVDKDFQCPKEKDTVKLEGIPGCKTCRFIVVMTARTYNQAPMSGLWLRLRPGVGRRAWMGCLWPLCHWLFSSLRTLGVSARCVNQGQVWIGGAVRGSGSCRRFYWLDGSAWDFWFWATGEPSASGGNCVSMYTQGGRWRLSQCDVVLPFVCSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MXF3</accession>
    <name>A0A8C4MXF3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Coiled-coil domain containing 88C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CCDC88C</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CCDC88 family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005033065.2">
        <property type="protein sequence ID" value="ENSEASP00005030414.2"/>
        <property type="gene ID" value="ENSEASG00005020710.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154785"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043296">
        <property type="term" value="C:apical junction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001965">
        <property type="term" value="F:G-protein alpha-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030165">
        <property type="term" value="F:PDZ domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003383">
        <property type="term" value="P:apical constriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044782">
        <property type="term" value="P:cilium organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030705">
        <property type="term" value="P:cytoskeleton-dependent intracellular transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001578">
        <property type="term" value="P:microtubule bundle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120197">
        <property type="term" value="P:mucociliary clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007026">
        <property type="term" value="P:negative regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031648">
        <property type="term" value="P:protein destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902691">
        <property type="term" value="P:respiratory basal cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060071">
        <property type="term" value="P:Wnt signaling pathway, planar cell polarity pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd22228">
        <property type="entry name" value="HkD_Daple"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000035">
        <property type="entry name" value="girdin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043936">
        <property type="entry name" value="HOOK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947">
        <property type="entry name" value="HOOK PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18947:SF31">
        <property type="entry name" value="PROTEIN DAPLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19047">
        <property type="entry name" value="HOOK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116907">
        <property type="entry name" value="Hook domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="8">
        <location>
            <begin position="11"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="222"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1417"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1756"/>
            <end position="1944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1958"/>
            <end position="2024"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="458"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="606"/>
            <end position="934"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="965"/>
            <end position="1013"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1045"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1136"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="1216"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="229"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1417"/>
            <end position="1428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="1433"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1451"/>
            <end position="1466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1517"/>
            <end position="1537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1573"/>
            <end position="1604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1795"/>
            <end position="1816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1879"/>
            <end position="1896"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030414.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="2024" mass="228201" checksum="5686C97819B66737" modified="2023-09-13" version="2">MDVTVSELMELFLQSPLVTWVKTFGPFGSGSQDNLTMYMDLADGIFLNQIMLQIDPRPTNQRINKHVNNDVNLRIQNLTILVRNIKTYYQEVLQQLIVMNLPNVLMIGRDPLSGKSMEEIKKVLLLVLGCAVQCERKEEFIERIKQLDIETQAGIVAHIQEVTHNQENVFDLQWLELPDVAPEELEALSRNMVFHLRRLIDERDECTELIVDLTQERDYLQAQHPPSPVKSSSSESTPSPTSSLSSEDKQHLAVELADTKARLRRVRQELEEKTEQLVDTRHEVDQLVLELQKVKQENIQLAADARSARAYRDELDSLREKANRVERLEMELVRCKEKLHDVDFYKARMEELREDNIILIETKAMLEEQLTAARARGDKVHELEKENLQLKSKLHDLELDRDTDKKRIEELLEENMVLEIAQKQSMNESAHLGWELEQLSKNAELSDASRKSFVFELNECASSRILKLEKENQSLQSTIQGLRDASLALEESSLKCGELEKENQQLSKKIEKLQTQLEREKQSNQDLETLSEELIKEKEQLQSDMETLKADRARQIKDLEQEKDHLNQTVWSLRERSQVSSEARVKDIEKENKVLHQTVTETSSKLSKLEFEKQQLHRDLEQVKEKVERAEELERELRRLEKENEQLAKKVTSLKTATEKVDALERESRGLALENRKLRKSLDTLQNVSVQLEGLERDNKQLDEENLELRRMVETMRFTSTKMAQIERENQELEREKEELRKNVELLKALSKKSERLELSYQSVSAENLRLQQSLESSGQKAQALERELGELEAENQVLQRDLEALRLSNKQLERSEKDRKALEQEVAQLEKDKKLLEKETKRLWQQVELKDAVLDDSTAKLSAAEKESRTLDKELARCRDAASKLKELEKDNRDLTKQVTMHTRTLTTLREDLVLEKLKSQQLTSELDKLSQELEKVGLHKELLLQDDNSNGDIKYKILEGRNESALKTTLAQKEEKIAFLEAQVEEKANLNHQLQNELEVVKKECEMLRQNQEEGTHLQNSFKHPVGTLVTGHQGREPWGPSHKEATMELLRVKDRVIDLERNNAALQAEKQLLKDQLQHLETQNVAFSSQILTLQKQSAFLQEHNTALQTQTAKLQVENSTLSSQSASLTAQYTLLQNQQTAKESENETLQKQQEQLSAAYEALLQDHEHLGALHERQSAEYEALIRQHSCLKTLHRNLELEHKELSERHSDMLKHKAELDELEKVLHTEREALQQEQRTNAIATSENQRLRGELDRVNFLHHQLKGEYEELHAHTKELKTSLNNSQLELNRWQARFDELKEQHQSMDISLTKLDNHCELLARLKGNLEEENHHLLSQIQLLSQQNQMLLEQNMESKEQYHEEQKQYIDKLNALRRQKEKLEEKIMDQYKFYDPAPKKKNHWIGAKALVKLIKPKKEGSRERLRSTTDGPPWPSESPDPASPSASQPLRSQSENPETTPSGSNGAEERDPHNGPVGKGPGDPKPKRGSPHGGSLDRTDASADLTLKPWPLELGSRTCSTSAITTTPSSSTPISRHPGRTKGYNSDDSLCEPSLELEFTNHRQYASRPGSLESSRNASSDSSPLNLKGSSDQLHGRSESFSSEDLIPSRDTATQPQDASAPGRTALGRHEYPPPRNGPLAQEGIQKRGVAQPHAGARPCSASPSSEMVTLEEFLEESNRSSPTPDTPGCRDELLSDYFRKATELPAIGGQSGPPTRKEGAKMPTSFVAPTIKMAINASEGKVLKPGQCVKSNFRAAEAEAPPSALPRQAQPPQSLSLGRPRQATMPPTSHTPASRSASLSRAFSLASADLLRASGPEACKQESPQKPGGPEASGGRETGSHTLQSPTPPSCHSLARERTPLVGKAGGSCQGPGPRSRPLDMRRFSLAPPKEERLAPLQQSATVPALAAGGGGGCGSNSQLQRFSSAAAPAARTKTQTPPYSGEVAAVAPVRVGLSLSEGDRVPGQGHGEGLPAKSPGRSPDVAPHASRGPEDFGRGSGSKSTPASPEPGGDQQTVWYEYGCV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PJI3</accession>
    <name>A0A8C4PJI3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">BMS1 ribosome biosis factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">BMS1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. BMS1 subfamily.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005010533.2">
        <property type="protein sequence ID" value="ENSEASP00005009700.1"/>
        <property type="gene ID" value="ENSEASG00005006894.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153195"/>
    <dbReference type="OMA" id="KLHVPMV"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01882">
        <property type="entry name" value="BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000105">
        <property type="entry name" value="BMS1 ribosome biogenesis factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037875">
        <property type="entry name" value="Bms1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030387">
        <property type="entry name" value="G_Bms1/Tsr1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF2">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN BMS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51714">
        <property type="entry name" value="G_BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Bms1-type G" evidence="12">
        <location>
            <begin position="80"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="399"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1220"/>
            <end position="1281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="416"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="436"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="508"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1220"/>
            <end position="1230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1231"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1248"/>
            <end position="1258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1281" mass="146092" checksum="86AC275C4D8A8893" modified="2022-01-19" version="1">METKDQKKHRKKNSGPKAEKKRKRHLQDLQLGDEEDARKRNPKAFAVQSAVRMARSFHRTQDLKTKKHHIPVVDRTPLEPPPIVVVVMGPPKVGKSTLIQCLIRNFTRQKLTEIRGPVTIVSGKKRRLTIIECGCDINVMIDLAKVADLVLMLIDASFGFEMETFEFLNICQVHGFPKIMGVLTHLDSFKYNKQLKKTKKRLKHRFWTEVYPGAKLFYLSGMVHGEYQNQEIHNLGRFITVMKFRPLTWQTSHPYILVDRMEDLTNPEDIRTNIKCDRKVSLYGYLRGAHLKNRSQIHMPGVGDFAVSDVSFLPDPCALPEQQKKRCLNEKEKLVYAPLSGVGGVLYDKDAVYVDLGGSHGFQESDEARPTHELVQSLISTHSTIDAKMASSRVTLFSDSKPLGSEDIDNQGLWMPKEEKQMDLKTGRVRRKAIFGDEEDESGDSDDEDDEEMSEGDSLENSSSDDETEKEEDAEMADKEYMTGKGVKRQRLEEMEEDVEVDLPAFADSDDDLERSSEEEEAGEADESSEEEDSTSEGERDILESKAAGEGSKAGLSDSLNPEKSLTMKKAILTTSDSGHCTAEEAFASEDESEESSSLSTEGEDSENEEVTRKKIPESSQVVSGQKLGLENLINETSDVEDLLREEESYKEENKFPTETSGALKWKEDLSRKAAEAFLRQQQATPNLRKLIYGTVTEDNEEDDDDTREELGGLFRVSQPARECKHKADSLDCSRFHVEAPHDWDLEEVMNSIRDCFVTGKWEEDKDAAKILAEDEELYGDFEDLETGDVHKGKSGPDTQIEDVEEEVKEEIDRSTEESAKKKHLDKKRKLKEMFDAEYDEGESTYFDDLKGEMQKQAQLNRAEFEDQDDEARVQYEGFRPGMYVRIEIENVPCEFVLNFDPHYPIILGGLGNSEGNVGYVQMRLKKHRWYKKILKSRDPIIFSVGWRRFQTIPLYYIEDHNGRQRLLKYTPQHMHCGATFWGPITPQGTGFLAVQSVSGVMPDFRIAATGVVLDLDKSIKIVKKLKLTGFPFKIFKNTSFIKGMFNSALEVAKFEGAVIRTVSGIRGQIKKALRAPEGAFRASFEDKLLMSDIVFMRTWYPVSIPAFYNPVTSLLKPVGEKDTWSGMRTTGQLRLAHGVKLKPNKDSLYKPIMRQKKHFNSLHIPKALQKALPFKNKPKTQAKAGKMPKDRLRPAVVREPHERKILALLDALSTVHSQKMKKAKVQQHLHNKEHFKAKQKEEEEKLKRQKDLRKKLFRIQGQKERRNQKSSLKGPEEQSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PJK1</accession>
    <name>A0A8C4PJK1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ras-related GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RRAGB</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014682139.1">
        <property type="nucleotide sequence ID" value="XM_014826653.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PJK1"/>
    <dbReference type="SMR" id="A0A8C4PJK1"/>
    <dbReference type="Ensembl" id="ENSEAST00005010658.2">
        <property type="protein sequence ID" value="ENSEASP00005009811.2"/>
        <property type="gene ID" value="ENSEASG00005006973.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106821786"/>
    <dbReference type="KEGG" id="eai:106821786"/>
    <dbReference type="CTD" id="10325"/>
    <dbReference type="GeneTree" id="ENSGT00950000183031"/>
    <dbReference type="OrthoDB" id="10020193at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11384">
        <property type="entry name" value="RagA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.190:FF:000002">
        <property type="entry name" value="Ras-related GTP-binding protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000276">
        <property type="entry name" value="Ras-related GTP-binding protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039397">
        <property type="entry name" value="RagA/B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF4">
        <property type="entry name" value="RAS-RELATED GTP-BINDING PROTEIN B"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10 12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU367014"/>
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    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009811.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="374" mass="43280" checksum="18B0C7E9CF8E87DF" modified="2023-09-13" version="2">MEESDSEKKMEKENLGPKMDPPIGEPEGSLGWVLPNTAMKKKVLLMGKSGSGKTSMRSIIFANYIARDTRRLGATILDRLHSLQINSSLSTYSLVDSVGNTKTFDVEHSHVRFLGNLVLNLWDCGGQDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNSPDAKIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECSCFRTSIWDETLYKAWSSIVYQLIPNVQQLEMNLRNFAEIIEADEVLLFERATFLVISHYQCKEQRDAHRFEKISNIIKQFKLSCSKLAASFQSMEVRNSNFAAFIDIFTSNTYVMVVMSDPSIPSAATLINIRNARKHFEKLERVDGPKQCLLMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PK97</accession>
    <name>A0A8C4PK97_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Myristoylated alanine-rich C-kinase substrate</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">MARCKS</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MARCKS family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PK97"/>
    <dbReference type="Ensembl" id="ENSEAST00005012957.2">
        <property type="protein sequence ID" value="ENSEASP00005011923.2"/>
        <property type="gene ID" value="ENSEASG00005008354.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111419"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032432">
        <property type="term" value="C:actin filament bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042585">
        <property type="term" value="C:germinal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005080">
        <property type="term" value="F:protein kinase C binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051764">
        <property type="term" value="P:actin crosslink formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021915">
        <property type="term" value="P:neural tube development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022008">
        <property type="term" value="P:neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002101">
        <property type="entry name" value="MARCKS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14353:SF9">
        <property type="entry name" value="MYRISTOYLATED ALANINE-RICH C-KINASE SUBSTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14353">
        <property type="entry name" value="MYRISTOYLATED ALANINE-RICH C-KINASE SUBSTRATE MARCKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02063">
        <property type="entry name" value="MARCKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00963">
        <property type="entry name" value="MARCKS"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00826">
        <property type="entry name" value="MARCKS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00827">
        <property type="entry name" value="MARCKS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="6" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="92"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="140"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="193"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011923.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="307" mass="30264" checksum="E190B5928F216631" modified="2023-09-13" version="2">MGAQFSKTAAKGEAAGERPGEAAVASSPSKANGQENGHVKVNGDASPAAAEPGAKEELQANGSAPAADKEEPAAAGSGAASPGAAEKDEAAAGEAAAEGEAAEPGSPTAAEGEAASAASSTSSPKAEDGATPSPSNETPKKKKKRFSFKKSFKLSGFSFKKNKKEAGEGAEAEGAAGASTESGKDEAAGGGTAAAAEAGAASGEQAAAPGEEAAAGEEGAAGGEPQEAKPEEAAVAPEKPAASEEAKAAEEPAKAEEKAEEAAASLRLILRKILHAIRSKFSCGFSSEVFKPEIRNQQCQITYRKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PLX6</accession>
    <name>A0A8C4PLX6_EQUAS</name>
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        <submittedName>
            <fullName evidence="2">Transmembrane protein 253</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">TMEM253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_044621212.1">
        <property type="nucleotide sequence ID" value="XM_044765277.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_044765278.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_044765279.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PLX6"/>
    <dbReference type="Ensembl" id="ENSEAST00005016193.2">
        <property type="protein sequence ID" value="ENSEASP00005014885.1"/>
        <property type="gene ID" value="ENSEASG00005010410.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106833306"/>
    <dbReference type="CTD" id="643382"/>
    <dbReference type="GeneTree" id="ENSGT00400000023212"/>
    <dbReference type="OMA" id="PRLWKVQ"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038874">
        <property type="entry name" value="TMEM253"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37359">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 253"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37359:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 253"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="133"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="204" mass="22146" checksum="542FE5A99E8EE05D" modified="2022-01-19" version="1">MEERAGQQEEERRSLRLEKLQHWAKHRQSGHLLLLAVSQLWLAVAVVPFAVSVACLNSACHMATALPLGPGALGLLTGTVTLELRRAPRFWKVRAMMIFNTFNLILGFIAVVVEVMKTALGPASTAPSQLAGLLVLELSAEAFTLGGVLVSAYSLFLLSQRKPGCCRSQSLHYQELQEGLSELEEVPDLENGPRVASTANGTNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PR72</accession>
    <name>A0A8C4PR72_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Torsin-3A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Torsin family 3 member A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TOR3A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="Liu"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
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                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ClpA/ClpB family. Torsin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PR72"/>
    <dbReference type="Ensembl" id="ENSEAST00005026770.2">
        <property type="protein sequence ID" value="ENSEASP00005024683.2"/>
        <property type="gene ID" value="ENSEASG00005016774.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182888"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001211">
        <property type="entry name" value="Torsin family 3 member A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049337">
        <property type="entry name" value="TOR1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010448">
        <property type="entry name" value="Torsin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10760">
        <property type="entry name" value="TORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10760:SF3">
        <property type="entry name" value="TORSIN-3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21376">
        <property type="entry name" value="TOR1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06309">
        <property type="entry name" value="Torsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="159"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="16"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005024683.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="397" mass="44924" checksum="D17D3073FA0E8809" modified="2023-09-13" version="2">MLRRPRSPLRLRLLLPLPGAPGHGGDAGPGPGAEKTGSRWAWPAFQGLRERLRAAGTLSRRYWALFSCRVWPEHCEEDEEAPAGPLAWGRALLGQQYLDILTTWYCSFQDCCDSGDCRISNNFTGLKSDLSVRLHGQHLAQELVLRTVRGYLELPRPDKALVLSFHGWSGTGKNFVARILAENLYRDGLRSDCVKVFIAMFHFPHPTSVDLYKEQLTGQIRKTQERCHQTLFIFDEAEKLHPGLLEALRPHLGRQAPENHRAQSPRTIFLFLSNLGGSVINEVVLRLLKAGRPREDVALGDLQPRLRAAVGEAPDSGFGHSCLVKENLIDFFVPFLPLEYRHVRLCARDAFLSQKLLYTEELLDEIAKMMVYVPKEEQLFSSQGCKSISQRINYFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PU15</accession>
    <name>A0A8C4PU15_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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            <location evidence="2 14">Cell membrane</location>
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        <property type="entry name" value="Olfactory receptor"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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        <property type="entry name" value="Olfact_rcpt"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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            <dbReference type="Ensembl" id="ENSEASP00005030042.2"/>
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            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
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            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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                <person name="Wang"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="J."/>
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                <person name="Liu"/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="2">Belongs to the NIPA family.</text>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="EmrE-like"/>
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    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
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    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR12570:SF1">
        <property type="entry name" value="MAGNESIUM TRANSPORTER NIPA2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="130"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="171"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="203"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="235"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="280"/>
            <end position="299"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00007230"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030700.1"/>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="360" mass="39160" checksum="C4D1C01353D72818" modified="2022-01-19" version="1">MSQGHGKYDFYIGLGLAMSSSIFIGGSFILKKKGLLRLARKGSMRAGQGGHAYLKEWLWWAGLLSMGAGEVANFAAYAFAPATLVTPLGALSVLVSAILSSYFLNERLNLHGKIGCLLSILGSTVMVIHAPKEEEIETLNEMSHKLGDPGFVVFATLVVIVSLILIFVVGPRHGQTNILVYITICSVIGAFSVSCVKGLGIAIKELFAGKPVLRHPLAWILLLSLIVCVSTQINYLNRALDIFNTSIVTPIYYVFFTTSVLTCSAILFKEWQDMPVDDVIGTLSGFFTIIVGIFLLHAFKDVSFSLASLPVSLRKDEKAVNGNLSNMYEVLNNNEESLTCGIEQHTSENISRRNGNLTAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PUR2</accession>
    <name>A0A8C4PUR2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Homeobox protein goosecoid</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GSC</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family. Bicoid subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014698673.1">
        <property type="nucleotide sequence ID" value="XM_014843187.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PUR2"/>
    <dbReference type="Ensembl" id="ENSEAST00005034298.2">
        <property type="protein sequence ID" value="ENSEASP00005031555.1"/>
        <property type="gene ID" value="ENSEASG00005021484.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106832421"/>
    <dbReference type="KEGG" id="eai:106832421"/>
    <dbReference type="CTD" id="145258"/>
    <dbReference type="GeneTree" id="ENSGT00940000160635"/>
    <dbReference type="OMA" id="QCSCVPA"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021904">
        <property type="term" value="P:dorsal/ventral neural tube patterning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030900">
        <property type="term" value="P:forebrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042474">
        <property type="term" value="P:middle ear morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048644">
        <property type="term" value="P:muscle organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030178">
        <property type="term" value="P:negative regulation of Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014036">
        <property type="term" value="P:neural crest cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023019">
        <property type="term" value="P:signal transduction involved in regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000210">
        <property type="entry name" value="homeobox protein goosecoid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051440">
        <property type="entry name" value="Goosecoid-like_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46643:SF2">
        <property type="entry name" value="HOMEOBOX PROTEIN GOOSECOID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46643">
        <property type="entry name" value="HOMEOBOX PROTEIN GOOSECOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="11">
        <location>
            <begin position="158"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="160"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="213"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="240"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005031555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="256" mass="27869" checksum="46BE1A5CB4C4D183" modified="2022-01-19" version="1">MPASMFSIDNILAARPRCKDSVLPVAPSAAAPVVFPALHGDSLYGAGGGASSDYGAFYPGPVAPGSAGLPAAVGGSRLGYNNYFYGQLHVQAAPVGPACCGAVPPLGAQQCSCVPTPSGYEGPGSVLVSPVPHQMLPYMNVGTLSRTELQLLNQLHCRRKRRHRTIFTDEQLEALENLFQETKYPDVGTREQLARKVHLREEKVEVWFKNRRAKWRRQKRSSSEESENAEKWNKTSSKASPEKREEEGKSDVDSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PV35</accession>
    <name>A0A8C4PV35_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Astrotactin-2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">ASTN2</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="23">Interacts with ASTN1; the interaction is not calcium-dependent.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the astrotactin family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005036031.2">
        <property type="protein sequence ID" value="ENSEASP00005033056.1"/>
        <property type="gene ID" value="ENSEASG00005022580.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003140"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043533">
        <property type="term" value="F:inositol 1,3,4,5 tetrakisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007158">
        <property type="term" value="P:neuron cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001764">
        <property type="term" value="P:neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000288">
        <property type="entry name" value="Astrotactin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040685">
        <property type="entry name" value="Annexin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045574">
        <property type="entry name" value="ASTN1_2_Fn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045575">
        <property type="entry name" value="ASTN_1_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040510">
        <property type="entry name" value="ASTN_2_hairpin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026995">
        <property type="entry name" value="Astrotactin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16592">
        <property type="entry name" value="ASTROTACTIN-1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16592:SF2">
        <property type="entry name" value="ASTROTACTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18411">
        <property type="entry name" value="Annexin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19743">
        <property type="entry name" value="ASTN1_2_fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19441">
        <property type="entry name" value="ASTN_1_2_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18577">
        <property type="entry name" value="ASTN_2_hairpin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="21" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0967">Endosome</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="18 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0653">Protein transport</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 27" id="KW-0732">Signal</keyword>
    <keyword evidence="10 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="27">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034847731" description="Astrotactin-2" evidence="27">
        <location>
            <begin position="50"/>
            <end position="1289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="205"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="381"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="28">
        <location>
            <begin position="801"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="297"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="329"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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    <evidence type="ECO:0000256" key="20">
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            <dbReference type="ARBA" id="ARBA00023180"/>
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            <dbReference type="ARBA" id="ARBA00061421"/>
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            <dbReference type="ARBA" id="ARBA00065119"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00071694"/>
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    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="28">
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            <dbReference type="SMART" id="SM00457"/>
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    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005033056.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1289" mass="142630" checksum="927E4B9C8DAF1506" modified="2022-01-19" version="1">MAAAGARRSPGPGLGLRGRLSLGFHPAPPPPPPLLLLLLLLLPPPPLLAGATAAASREPDSPCRLKTVTVSTLPALRESDIGWSGARAGAGTGTGAGAAAAAAASPGAAGSTGTAAESRLLLFVRNELPGRIAVQDDLDNTELPFFTLEMSGTAADISLVHWRQQWLENGTLYFHVSMSSSGQLARATAPTLQEPSEIVEEQMHILHISVMGGLIALLLLLLVFTVALYAQRRWQKRRRIPQKSASTEATHEIHYIPSVLLGPQARESFRSSRLQAHNSVIGVPIRETPILDDYDYEEEEDPPRRANHVSREDEFGSQVTHTLDSLGRPGEEKGDFEKKGGISFGRTKGMSGSEADDETQLTFYTEQYRSRRRSKGLLKSPVNKTALTLIAVSSCILAMVCGSQMSCPLTVKVTLHVPEHFIADGSSFVVSEGSYLDISDWLNPAKLSLYYQINATSPWVRDLCGQRTTDACEQLCDPETGECSCHEGYAPDPVHRHLCVRSDWGQSEGPWPYTTLERGYDLVTGEQAPEKILRSTFSLGQGLWLPVSKSFVVPPVELSINPLASCKTDVLVTEDPADVREEAMLSTYFETINDLLSSFGPVRDCSRNNGGCTRNFKCVSDRQVDSSGCVCPEELRPMKDGSGCYDHSKGIDCSDGFNGGCEQLCLQQTLPLPYDATSSTIFMFCGCVEEYKLAPDGKSCLMLSDVCEGPKCLKPDSKFNDTLFGEMLHGYNNRTQHVNQGQVFQMTFRENNFIKDFPQLADGLLVIPLPVEEQCRGVLSEPLPDLQLLTGDIRYDEAMGYPMVQQWRVRSNLYRVKLSTITLSAGFTNVLKILTKESSREELLSFIQHYGSHYIAEALYGSELTCIIHFPSKKVQQQLWLQYQKETTELGSKKELKSMPFITYLSGLLTAQMLSDDQLISGVEIRCEEKGRCPSTCHLCRRPGKEQLSPTPVLLEINRVVPLYTLIQDNGTKEAFKSALMSSYWCSGKGDVIDDWCRCDLSAFDASGLPNCSPLPQPVLRLSPTVEPSSTVVSLEWVDVQPAIGTKVSDYILQHKKVDEYTDTDLYTGEFLSFADDLLSGLGTSCVAAGRSHGEVPEVSIYSVIFKCLEPDGLYKFTLYAVDTRGRHSELSTVTLRTACPLVDDNKAEEIADKIYNLYNGYTSGKEQQTAYNTLMEVSASMLFRVQHHYNSHYEKFGDFVWRSEDELGPRKAHLILRRLERVSNHCSSLLRSAYIQSRVDTVPYLFCRSEEVRPAGMVWYSILKDTKITCEEKMVSMARNTYGESKGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I4R0</accession>
    <name>A0A9L0I4R0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">adenylate cyclase</fullName>
            <ecNumber evidence="5">4.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 22">
            <text>ATP = 3',5'-cyclic AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15389"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="EC" id="4.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.1" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0I4R0"/>
    <dbReference type="Ensembl" id="ENSEAST00005051892.1">
        <property type="protein sequence ID" value="ENSEASP00005034986.1"/>
        <property type="gene ID" value="ENSEASG00005036818.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154872"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006171">
        <property type="term" value="P:cAMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07302">
        <property type="entry name" value="CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1230:FF:000001">
        <property type="entry name" value="Adenylate cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001054">
        <property type="entry name" value="A/G_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018297">
        <property type="entry name" value="A/G_cyclase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45627:SF26">
        <property type="entry name" value="ADENYLATE CYCLASE TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00211">
        <property type="entry name" value="Guanylate_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00044">
        <property type="entry name" value="CYCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55073">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00452">
        <property type="entry name" value="GUANYLATE_CYCLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50125">
        <property type="entry name" value="GUANYLATE_CYCLASE_2"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="component" value="Chromosome 1"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="T cell receptor gamma constant 2"/>
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        <property type="entry name" value="Uncharacterized protein"/>
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        <property type="entry name" value="Immunoglobulins"/>
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        <property type="entry name" value="TRG_var/const_region"/>
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        <property type="entry name" value="IGc1"/>
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        <source>
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            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005036337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="334" mass="37945" checksum="D13289BFB1CF73EF" modified="2023-09-13" version="1">MSLLEAFTFLSFWAVGLGLTKVEQSQISISAEVKKSIDIHCKITSSNFEGEYIHWYRRKTYEALEHLIYVSSTKSPAQANLGGKKNKIEARKDSQTSTAILTVNFIENEDVAIYYCTGWYPHSSWIKIFGKGTKLVVTPSDISLPADISPKPTSFLPSIAEIKLHKAGTYLCLLEKFFPDVIKIYWKEKDGSTIVESQEGTTVKTNDTYMKFSWLTVTENSMDKEHKCIVQHENNKRGIDQEIPFPSIKKVVAAFDSTKTCVTDEISPLQLQLTSTSAYYTYLLLLLKSVIYLAIITFCLFRRTAVCGDGKSSNRRWRKKVNFSSSFIVRKRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I8T9</accession>
    <name>A0A9L0I8T9_EQUAS</name>
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        <recommendedName>
            <fullName evidence="14">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106835724</name>
    </gene>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014703783.1">
        <property type="nucleotide sequence ID" value="XM_014848297.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A9L0I8T9"/>
    <dbReference type="Ensembl" id="ENSEAST00005077321.1">
        <property type="protein sequence ID" value="ENSEASP00005036997.1"/>
        <property type="gene ID" value="ENSEASG00005034718.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106835724"/>
    <dbReference type="KEGG" id="eai:106835724"/>
    <dbReference type="GeneTree" id="ENSGT00940000161377"/>
    <dbReference type="OrthoDB" id="9975554at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15225">
        <property type="entry name" value="7tmA_OR10A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000151">
        <property type="entry name" value="olfactory receptor 10K1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="98"/>
            <end position="120"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="141"/>
            <end position="166"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="197"/>
            <end position="226"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="238"/>
            <end position="260"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005036997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="313" mass="34995" checksum="5CBAB75113990CE1" modified="2023-09-13" version="1">MERVNETLVAEFVFLGFSSLARMQLLLFIVFLLLYLFTLGTNTIIISTIVLDRALHNPMYFFLTVLSCSETCYTFVIVPKMLADLLAQKKTITFLGCAIQMFSFLFLGCSHSFLLAAMGYDRYMAICNPLQYTVLMGHGMCVGLVAAACACGFTVSLVTTSLVFYLPFHSSNQLHHFFCDISPVLKLASHHSRLSQLVIFMLGVCVLVIPLLLILVSYIHIISAILKISTSAGRYKTFSTCASHLIVVTVHYGCASFIYLRPKSNYSSSQDILISVSYTILTPLFNPMIYSLRNKEFKSAFRRVMGQTSCLVN</sequence>
</entry>
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    <accession>A0A9L0I983</accession>
    <name>A0A9L0I983_EQUAS</name>
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            <fullName evidence="15">Nectin-1</fullName>
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        <alternativeName>
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            <fullName evidence="18">Poliovirus receptor-related protein 1</fullName>
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            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nectin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0I983"/>
    <dbReference type="Ensembl" id="ENSEAST00005049139.1">
        <property type="protein sequence ID" value="ENSEASP00005037234.1"/>
        <property type="gene ID" value="ENSEASG00005003916.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156933"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001618">
        <property type="term" value="F:virus receptor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902414">
        <property type="term" value="P:protein localization to cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05890">
        <property type="entry name" value="IgC1_2_Nectin-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05886">
        <property type="entry name" value="IgV_1_Nectin-1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000268">
        <property type="entry name" value="Nectin cell adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000304">
        <property type="entry name" value="Nectin cell adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000427">
        <property type="entry name" value="Nectin cell adhesion molecule 1"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832:SF287">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13832">
        <property type="entry name" value="PROTEIN PHOSPHATASE 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005037421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="189" mass="21033" checksum="B1C3ABD67DE6FA99" modified="2023-09-13" version="1">MAMGKARLQSLFRGKLTCEGGSASLRSFIHPFRAYKTIEDDDLKFPLIYGEGKKARVMATIGVTRGLGDHDLKVHDSNIYIKPFLSSAPEVRIYDLSKYEHGADDVLILATDGLWDVLSNEEVAEAITQFLPNCDPDDPHRYTLAAQDLVMRARGVLKDRGWRISNDRLGSGDDISVYVIPLVHGNKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IE01</accession>
    <name>A0A9L0IE01_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">L-Fucosyltransferase</fullName>
            <ecNumber evidence="26">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">FUT2</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Catalyzes the transfer of L-fucose, from a guanosine diphosphate-beta-L-fucose, to the terminal galactose on both O- and N-linked glycans chains of cell surface glycoproteins and glycolipids and the resulting epitope regulates several processes such as cell-cell interaction including host-microbe interaction, cell surface expression and cell proliferation. Preferentially fucosylates gangliosides GA1 and GM1 in the antrum, cecum and colon and in the female reproductive organs. Fucosylated host glycoproteins or glycolipids mediate interaction with intestinal microbiota influencing its composition. Creates a soluble precursor oligosaccharide FuC-alpha ((1,2)Galbeta-) called the H antigen which is an essential substrate for the final step in the soluble ABO blood group antigen synthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>Lc4Cer + GDP-beta-L-fucose = alpha-L-fucosyl-(1-&gt;2)-beta-D-galactosyl-(1-&gt;3)-N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-beta-D-glucosyl-(1&lt;-&gt;1')-ceramide + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48792"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:90800"/>
            <dbReference type="ChEBI" id="CHEBI:90802"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:48793"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a beta-D-Gal-(1-&gt;3)-beta-D-GlcNAc-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Glc-(1&lt;-&gt;1')-Cer(d18:1(4E)) + GDP-beta-L-fucose = alpha-L-fucosyl-(1-&gt;2)- beta-D-galactosyl-(1-&gt;3)-N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-beta-D-galactosyl-(1-&gt;4)-beta-D-glucosyl-(1&lt;-&gt;1')-N-acylsphing-4-enine + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32175"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17292"/>
            <dbReference type="ChEBI" id="CHEBI:28743"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="2.4.1.69"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:32176"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a beta-D-galactosyl-(1-&gt;3)-N-acetyl-beta-D-glucosaminyl derivative + GDP-beta-L-fucose = an alpha-L-Fuc-(1-&gt;2)-beta-D-Gal-(1-&gt;3)-beta-D-GlcNAc derivative + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50664"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:133506"/>
            <dbReference type="ChEBI" id="CHEBI:133509"/>
            <dbReference type="EC" id="2.4.1.69"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:50665"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl derivative + GDP-beta-L-fucose = an alpha-L-Fuc-(1-&gt;2)-beta-D-Gal-(1-&gt;4)-beta-D-GlcNAc derivative + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50668"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:133507"/>
            <dbReference type="ChEBI" id="CHEBI:133510"/>
            <dbReference type="EC" id="2.4.1.344"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:50669"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a ganglioside GA1 + GDP-beta-L-fucose = a ganglioside Fuc-GA1 + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48320"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:88069"/>
            <dbReference type="ChEBI" id="CHEBI:90262"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:48321"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a ganglioside GD1b + GDP-beta-L-fucose = a ganglioside Fuc-GD1b + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48324"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:82939"/>
            <dbReference type="ChEBI" id="CHEBI:90265"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:48325"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>a ganglioside GM1 (d18:1(4E)) + GDP-beta-L-fucose = a ganglioside Fuc-GM1 (d18:1(4E)) + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42040"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:77709"/>
            <dbReference type="ChEBI" id="CHEBI:78607"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:42041"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a ganglioside GM1 + GDP-beta-L-fucose = a ganglioside Fuc-GM1 + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:82639"/>
            <dbReference type="ChEBI" id="CHEBI:90189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:48293"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a globoside GalGb4Cer (d18:1(4E)) + GDP-beta-L-fucose = a globoside Globo-H (d18:1(4E)) + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42044"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:62571"/>
            <dbReference type="ChEBI" id="CHEBI:62649"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:42045"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a lactoside III(4)-a-Fuc-Lc4Cer + GDP-beta-L-fucose = a lactoside IV(2),III(4)-a-[Fuc]2-Lc4Cer + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:62616"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:90811"/>
            <dbReference type="ChEBI" id="CHEBI:142612"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:62617"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a neolactoside nLc4Cer + GDP-beta-L-fucose = a neolactoside IV(2)-alpha-Fuc-nLc4Cer + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48800"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:90376"/>
            <dbReference type="ChEBI" id="CHEBI:90803"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:48801"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a neolactoside nLc4Cer(d18:1(4E)) + GDP-beta-L-fucose = a neolactoside IV(2)-alpha-Fuc-nLc4Cer(d18:1(4E)) + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48304"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17006"/>
            <dbReference type="ChEBI" id="CHEBI:28691"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:48305"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>beta-D-galactosyl-(1-&gt;3)-N-acetyl-D-galactosamine + GDP-beta-L-fucose = alpha-L-fucosyl-(1-&gt;2)-beta-D-galactosyl-(1-&gt;3)-N-acetyl-D-galactosamine + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:62964"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:84728"/>
            <dbReference type="ChEBI" id="CHEBI:546807"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:62965"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="5 26">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 26">Golgi apparatus</location>
            <location evidence="4 26">Golgi stack membrane</location>
            <topology evidence="4 26">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="26">Belongs to the glycosyltransferase 11 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="26"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0IE01"/>
    <dbReference type="Ensembl" id="ENSEAST00005045624.1">
        <property type="protein sequence ID" value="ENSEASP00005039144.1"/>
        <property type="gene ID" value="ENSEASG00005036881.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005049657.1">
        <property type="protein sequence ID" value="ENSEASP00005054905.1"/>
        <property type="gene ID" value="ENSEASG00005036881.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005060475.1">
        <property type="protein sequence ID" value="ENSEASP00005046843.1"/>
        <property type="gene ID" value="ENSEASG00005036881.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005061595.1">
        <property type="protein sequence ID" value="ENSEASP00005047571.1"/>
        <property type="gene ID" value="ENSEASG00005036881.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001450"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008107">
        <property type="term" value="F:galactoside 2-alpha-L-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006664">
        <property type="term" value="P:glycolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009312">
        <property type="term" value="P:oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd11301">
        <property type="entry name" value="Fut1_Fut2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002516">
        <property type="entry name" value="Glyco_trans_11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11927">
        <property type="entry name" value="GALACTOSIDE 2-L-FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11927:SF2">
        <property type="entry name" value="GALACTOSIDE ALPHA-(1,2)-FUCOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01531">
        <property type="entry name" value="Glyco_transf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 26" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 26" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 26" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 26" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 26" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 26" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00043729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00043736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00043755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00043824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00043835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00046221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00047716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU363129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="346" mass="39438" checksum="DC3018462CDF162E" modified="2023-09-13" version="1">MLSTQAPFFFPMGHFILFVFVASTIFHIQQRLAKIQRTGELVPAIRPALETMERTTTNQPPIPRGMWTINAIGRLGNQMGEYATLYALAKMNGRPAYIPAQMHNALAPIFRITLPVLHGDTASRVPWRNYHLNDWMEERYRHIPGDYVRLTGYPCSWTFYHHLRQEILQEFTLHDHVREEAQKYLRGLQVNGSRPSTFVGVHVRRGDYVRVMPQVWKGVVADRRYLEQALDWFRARYSSVVFVVTSNGMAWCQENIDASHGDVVFAGDGKEGSPAKDFALLTQCNHTVMTIGTFGIWAAYLAGGETIYLANYTLPDSPFLKVFKPEAAFLPEWIGIPADLSPLLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IEG6</accession>
    <name>A0A9L0IEG6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WD repeat domain phosphoinositide-interacting protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">WIPI2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Involved in an early step of the formation of preautophagosomal structures. Binds and is activated by phosphatidylinositol 3-phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases. Mediates ER-isolation membranes contacts by interacting with the ULK1:RB1CC1 complex and PtdIns3P. Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with TECPR1. Interacts with ATG16L1. Interacts with ATG5. Interacts with WIPI1. Interacts with WDR45. May interact with NUDC. Interacts with ULK1 and RB1CC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Preautophagosomal structure membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the WD repeat PROPPIN family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IEG6"/>
    <dbReference type="Ensembl" id="ENSEAST00005066248.1">
        <property type="protein sequence ID" value="ENSEASP00005039320.1"/>
        <property type="gene ID" value="ENSEASG00005021536.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 14"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="C:phagophore assembly site membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:cellular response to starvation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:protein localization to phagophore assembly site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000145">
        <property type="entry name" value="WD repeat domain phosphoinositide-interacting protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048720">
        <property type="entry name" value="PROPPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11227">
        <property type="entry name" value="WD-REPEAT PROTEIN INTERACTING WITH PHOSPHOINOSIDES WIPI -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21032">
        <property type="entry name" value="PROPPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0072">Autophagy</keyword>
    <keyword evidence="6" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0853">WD repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00056098"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00062217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="433" mass="47269" checksum="1CE3BB9E2E4745DC" modified="2023-09-13" version="1">MNLASQSGEAGAGQLLFANFNQDNTSLAVGSKSGYKFFSLSSVDKLEQIYECTDTEDVCIVERLFSSSLVAIVSLKAPRKLKVCHFKKGTEICNYSYSNTILAVKLNRQRLIVCLEESLYIHNIRDMKVLHTIRETPPNPAGLCALSINNDNCYLAYPGSATIGEVQVFDTINLRAANMIPAHDSPLAALAFDASGTKLATASEKGTVIRVFSIPEGQKLFEFRRGVKRCVSICSLAFSMDGMFLSASSNTETVHIFKLEAVKEKPQEEPTTWTGYFGKVLMASTSYLPSQVTEMFNQGRAFATVRLPFCGHKNICSLATIQKIPRLLVGASDGYLYMYNLDPQEGGECTLMKQHKLDGSMETTNEILDSASHDCPLVTPTYGAAGAHAPSSPTRLAYTDELGAAGSTCLEDEASALRLEEDSEHPPMILRTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IEM3</accession>
    <name>A0A9L0IEM3_EQUAS</name>
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        <submittedName>
            <fullName evidence="3">Serglycin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SRGN</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Perissodactyla</taxon>
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        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IEM3"/>
    <dbReference type="Ensembl" id="ENSEAST00005056840.1">
        <property type="protein sequence ID" value="ENSEASP00005039375.1"/>
        <property type="gene ID" value="ENSEASG00005024600.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000885"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042629">
        <property type="term" value="C:mast cell granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0140507">
        <property type="term" value="P:granzyme-mediated programmed cell death signaling pathway"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:maintenance of granzyme B location in T cell secretory granule"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:maintenance of protease location in mast cell secretory granule"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0030502">
        <property type="term" value="P:negative regulation of bone mineralization"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0001818">
        <property type="term" value="P:negative regulation of cytokine production"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007455">
        <property type="entry name" value="Serglycin"/>
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    <dbReference type="PANTHER" id="PTHR17178">
        <property type="entry name" value="SECRETORY GRANULE PROTEOGLYCAN CORE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17178:SF0">
        <property type="entry name" value="SERGLYCIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04360">
        <property type="entry name" value="Serglycin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040386037" evidence="2">
        <location>
            <begin position="28"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="156" mass="17213" checksum="20ECF1E0CCB43240" modified="2023-09-13" version="1">TMQVLLQGSRLVLALTLILVLSSSVQGFPVRRARYQWVRCSPDSNSANCIDERGPVFDLLPDEPNRILPPRTDPFSMTRSQNLNDVFPLSEDNSGSGFGSGSGSGSESSFLTDIEQEYQPADENNAFLYNFRSLEGNLPSDNQDLGQDGSEENFIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IFD4</accession>
    <name>A0A9L0IFD4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Aspartate--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="4">6.1.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Aspartyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">DARS1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>tRNA(Asp) + L-aspartate + ATP = L-aspartyl-tRNA(Asp) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19649"/>
            <dbReference type="Rhea" id="RHEA-COMP:9660"/>
            <dbReference type="Rhea" id="RHEA-COMP:9678"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homodimer. Part of a multisubunit complex that groups tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.12" evidence="4"/>
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        <property type="nucleotide sequence ID" value="XM_070507603.1"/>
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        <property type="gene ID" value="ENSEASG00005007349.2"/>
    </dbReference>
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    <dbReference type="CTD" id="1615"/>
    <dbReference type="GeneTree" id="ENSGT01030000234618"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04320">
        <property type="entry name" value="AspRS_cyto_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000013">
        <property type="entry name" value="Aspartate--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003483">
        <property type="entry name" value="PRK05159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450:SF1">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="ASPARTYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="15">
        <location>
            <begin position="165"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="470" mass="53493" checksum="01C73ACB2A30056F" modified="2023-09-13" version="1">MPSADASRKSQEKPREIMDAAEDFAKERYGVSSMIQSQEKPGKQCFLVLRQQQFNVQALVAVGDHASKQMVKFAANINKESIVDVEGVVRKVNQKIGSCTQQDVELHVQKIYVISLAEPRLPLQLDDAVRPEVEGEEEGRATVNQDTRLDNRVIDLRTSTSQAVFRLQSGICHLFRETLINKGFVEIQTPKIISAASEGGANVFTVSYFKNNAYLAQSPQLYKQMCICADFEKVFCIGPVFRAEDSNTHRHLTEFVGLDIEMAFSYHYHEVVEEIADTLVQIFKGLQERFQTEIQTVNKQFPCEPFKFLEPTLRLEYCEALAMLREAGIEMGDEEDLSTPNEKLLGRLVKEKYDTDFYILDKYPLAVRPFYTMPDPRNPKQSNSYDMFMRGEEILSGAQRIHDPQLLTERALHHGIDLEKIKAYIDSFRFGAPPHAGGGIGLERVTMLFLGLHNVRQTSMFPRDPKRLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IM31</accession>
    <name>A0A9L0IM31_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Histone H2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106837835</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
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                <person name="Yang"/>
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                <person name="Liu"/>
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                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <property type="project" value="UniProtKB-KW"/>
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            <begin position="20"/>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF00125"/>
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            <dbReference type="Ensembl" id="ENSEASP00005038123.1"/>
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</entry>
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                <person name="Liu"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Li"/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
                <person name="J."/>
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                <person name="Ji"/>
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                <person name="Jin"/>
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                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
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                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="5" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="similarity">
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        <property type="gene ID" value="ENSEASG00005038465.1"/>
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    <dbReference type="OMA" id="PPIIEEW"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="PANTHER" id="PTHR48168">
        <property type="entry name" value="RNA GUANINE-7 METHYLTRANSFERASE-ACTIVATING SUBUNIT-LIKE (PSEUDOGENE)-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="25"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="57"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005043198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="118" mass="14201" checksum="54F75397DE3DA1FD" modified="2023-09-13" version="1">MTDTSEAVPSFEEMFASRFTEDDKEYQEYLKRPPESPPIVEEWNSRAGGSHRNRGNRLQDNRQFRGRDGRRGWPSDNRSNQWHGRSWGNNYPQHRQEPYYPHQYGHCGYNQRPPYGYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IUB7</accession>
    <name>A0A9L0IUB7_EQUAS</name>
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        <submittedName>
            <fullName evidence="7">INO80 complex subunit C</fullName>
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    <gene>
        <name evidence="7" type="primary">INO80C</name>
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    <organism evidence="7 8">
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Mammalia</taxon>
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            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
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                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014684849.1">
        <property type="nucleotide sequence ID" value="XM_014829363.3"/>
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    <dbReference type="Ensembl" id="ENSEAST00005072100.1">
        <property type="protein sequence ID" value="ENSEASP00005044561.1"/>
        <property type="gene ID" value="ENSEASG00005016596.2"/>
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    <dbReference type="KEGG" id="eai:106823557"/>
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        <property type="term" value="C:MLL1 complex"/>
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        <property type="term" value="P:chromatin remodeling"/>
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        <property type="term" value="P:regulation of embryonic development"/>
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        <property type="entry name" value="INO80C/Ies6"/>
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        <property type="entry name" value="Vps72/YL1_C"/>
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        <property type="entry name" value="INO80 COMPLEX SUBUNIT C"/>
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        <property type="entry name" value="YL1_C"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="182"/>
            <end position="211"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Plays a role in the early steps of cytochrome c oxidase subunit II (MT-CO2/COX2) maturation and is required for the stabilization of COX20 and the newly synthesized MT-CO2/COX2 protein.</text>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005045156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="311" mass="33568" checksum="9A39007710AE35E8" modified="2023-09-13" version="1">MAGPLWRASAFVQRHRTGLLVGSCAGLFGAQISYHLFPDPVVQWLYQYWPQGQPAPLSPELQRLFQEVLQDMGVPSGHCYEAFTTFTFQPVSAGFPRLPAGAVVGIPASFLGGPVTHSDQPVIVHGHRVNWQSPADARLRDALTLSRDAQKFALAREVVYLESAAAALQALPAPACLAGTWALGVGAKHDLGLYGGPMNLRAAFNLVAAVAGFAAYAFSTDSLTHALEAWLDHLTASLSAAYARGGVEFNEKVLSGILALRGLLGWPGEKLYTASGNVAPRHWFRVKHLPYTTRRDSVLQLWRVMLTPGSS</sequence>
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    <name>A0A9L0IZJ9_EQUAS</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IZJ9"/>
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        <property type="protein sequence ID" value="ENSEASP00005045548.1"/>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
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                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JD80"/>
    <dbReference type="Ensembl" id="ENSEAST00005072496.1">
        <property type="protein sequence ID" value="ENSEASP00005050313.1"/>
        <property type="gene ID" value="ENSEASG00005037453.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00900000143813"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="82" mass="9485" checksum="DBA80BEB08B542A9" modified="2023-09-13" version="1">MCEIDSLASTFVPNLCGGPKGFPFSLKNFFFRLYEFRVSLNYLYAFIFSNSYDDGLSYFISSDFLLVLEGVFSFLSITFFFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JEW9</accession>
    <name>A0A9L0JEW9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Regulator of chromosome condensation</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Chromosome condensation protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">RCC1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP, and thereby plays an important role in RAN-mediated functions in nuclear import and mitosis. Contributes to the generation of high levels of chromosome-associated, GTP-bound RAN, which is important for mitotic spindle assembly and normal progress through mitosis. Via its role in maintaining high levels of GTP-bound RAN in the nucleus, contributes to the release of cargo proteins from importins after nuclear import. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with RAN. Interacts with KPNA3. Interacts (via N-terminus and RCC1 repeats) with KPNA4. Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JEW9"/>
    <dbReference type="Ensembl" id="ENSEAST00005069613.1">
        <property type="protein sequence ID" value="ENSEASP00005050883.1"/>
        <property type="gene ID" value="ENSEASG00005010059.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155543"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031492">
        <property type="term" value="F:nucleosomal DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043199">
        <property type="term" value="F:sulfate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007088">
        <property type="term" value="P:regulation of mitotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000008">
        <property type="entry name" value="regulator of chromosome condensation isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF9">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00625">
        <property type="entry name" value="RCC1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="21" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="19">
        <location>
            <begin position="92"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="22">
        <location>
            <begin position="94"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="19">
        <location>
            <begin position="143"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="19">
        <location>
            <begin position="195"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="19">
        <location>
            <begin position="248"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="19">
        <location>
            <begin position="316"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="19">
        <location>
            <begin position="370"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="19">
        <location>
            <begin position="421"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="56"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="76"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00054165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00064365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00080842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF25390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="478" mass="51862" checksum="807D7CBAFCAEE470" modified="2023-09-13" version="1">MTEETNHAGNIYYTICSLSKMVNMSLILYFFFFFFFHSHSGEKNLTTKLWQRDLDRNMPPKRIAKRRSPPEDALPKSKKAKVSHRSHSTEPGLVLTLGQGDVGQLGLGENVLERKKPALVAIPEDVVQAEAGGMHTVCLSKSGQVYSFGCNDEGALGRDTSVEGSEMVPGKVELQEKVVQVSAGDSHTAALTEDGRVFLWGSFRDNNGVIGLLEPMKKSMVPVQVQLSMPVVKVASGNDHLVMLTADGDLYTLGCGEQGQLGRVPELFANRGGRQGLERLLVPKCVMLKSRGSRGHVRFQDAFCGAYFTFAISHEGHVYGFGLSNYHQLGTPGTESCFVPQNLTSFKNSTKSWVGFSGGQHHTVCMDSEGRAYSLGRAEYGRLGLGEGAEEKSIPTLISRLPAVSSVACGASVGYAVTKDGRVFAWGMGTNYQLGTGQEEDAWSPVEMTGKQLENRVVLSVSSGGQHTVLLVKDKEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JGE6</accession>
    <name>A0A9L0JGE6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Histone-lysine N-methyltransferase SETD2</fullName>
            <ecNumber evidence="3">2.1.1.359</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="31">Lysine N-methyltransferase 3A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="32">Protein-lysine N-methyltransferase SETD2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">SET domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="38" type="primary">SETD2</name>
    </gene>
    <organism evidence="38 39">
        <name type="scientific">Equus asinus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="38 39" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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                <consortium name="Ensembl"/>
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        <reaction evidence="27">
            <text>L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
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    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>L-lysyl-[protein] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl-[protein] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
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    <comment type="catalytic activity">
        <reaction evidence="28">
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            <begin position="2438"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="33">
        <location>
            <begin position="1"/>
            <end position="11"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="33">
        <location>
            <begin position="17"/>
            <end position="30"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="33">
        <location>
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<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
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                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
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                <person name="Q."/>
                <person name="Ji"/>
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                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="2">
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            <begin position="358"/>
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    </feature>
    <feature type="domain" description="EamA" evidence="3">
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            <begin position="69"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="394"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="412"/>
            <end position="422"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005048530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="422" mass="46734" checksum="5E3851FE79296348" modified="2023-09-13" version="1">MKKHSARVAPLSACNSPVLTLTKVEGEERPRDSPGPAEAQAPAAGAEAGGRGSRRCWRCSRAQLKKIFWGVAVVLCVCSSWAGSTQLAKLTFRKFDAPFTLTWFATNWNFLFFPLYYAGHVCKSAEKQSVKQRYRECCRFFGDNGLTLKVFFTKAAPFGVLWTLTNYLYLHAIKKINTTDVSVLFCCNKAFVFLLSWIVLRDRFMGVRIVAAILAIAGIVMMTYADGFHSHSVIGIALVVGSASMSALYKVLFKLLLGSAKFGEAALFLSILGVFNILFITCIPVILYFTKVEYWSSFDDIPWGNLCGFSVLLLTFNIVLNFGIAVTYPTLMSLGIVLSVPVNAVVDHYTSKIVFNGVRVIAIIIIGLGFLLLLLPEEWDVWLIKLLTRLKVRKKEETAEGSADPGSGPQSKSRRARPSFAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JJH7</accession>
    <name>A0A9L0JJH7_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Activating transcription factor 7 interacting protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ATF7IP2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCAF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014705421.1">
        <property type="nucleotide sequence ID" value="XM_014849935.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014705422.1">
        <property type="nucleotide sequence ID" value="XM_014849936.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014705424.1">
        <property type="nucleotide sequence ID" value="XM_014849938.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044602803.1">
        <property type="nucleotide sequence ID" value="XM_044746868.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070341041.1">
        <property type="nucleotide sequence ID" value="XM_070484940.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070341042.1">
        <property type="nucleotide sequence ID" value="XM_070484941.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070341043.1">
        <property type="nucleotide sequence ID" value="XM_070484942.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070341044.1">
        <property type="nucleotide sequence ID" value="XM_070484943.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JJH7"/>
    <dbReference type="Ensembl" id="ENSEAST00005065040.1">
        <property type="protein sequence ID" value="ENSEASP00005052513.1"/>
        <property type="gene ID" value="ENSEASG00005026025.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106836761"/>
    <dbReference type="KEGG" id="eai:106836761"/>
    <dbReference type="CTD" id="80063"/>
    <dbReference type="GeneTree" id="ENSGT00530000063707"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026085">
        <property type="entry name" value="ATF7-int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031870">
        <property type="entry name" value="ATF7IP_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056565">
        <property type="entry name" value="Fn3_ATF7IP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23210">
        <property type="entry name" value="ACTIVATING TRANSCRIPTION FACTOR 7 INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23210:SF23">
        <property type="entry name" value="ACTIVATING TRANSCRIPTION FACTOR 7-INTERACTING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16788">
        <property type="entry name" value="ATF7IP_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16794">
        <property type="entry name" value="fn3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="580"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="274"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="412"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="464"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="687" mass="76677" checksum="2850504728D24706" modified="2023-09-13" version="1">MASPDRSKRKILKAKKTMPASCRKQVAILKKSKNVEALKTAATGSNVSSGNLNSSSGVISSKCKRSESDASSLDSNKNSVCSQESKVSSRNLIKPLEIVDSETRLNYVEEQIACPYQKPSKTIESLRKLFTQEAQDSQNTSENHLQCQANITRSFFEHEENCNLKCIWHPSTVLNGTVQMSEFTVTNTLDDKRIDKMVSYLETNSNFESRDKRQNDILCSDTCSLPLDKIPKLVNSVISSNCAADVLKIEESCRTCHSSIDYESMGSTCLSSLDTDSNNSHNQKKRMFSENKENVKRRKTSEQISENICVVLEKQTALLEQVKQLIRQEICSINYKLFDNRLKELTERIGKTQCRNKHEAIADALFAKIAKLQRRIRAVLSSQRNCLETNVLSSNIACKVANAEIMNLDMNPVSLKSPDERKTSVDSKPSNPSEEASEEINFSREHDEAVSESNNDDVMLISVESPNLTPPITSNPTDTGKITSGNSNHSPDAETEVMAVEKNKLDSVIDLTKEGPSDSTAVSPVSTLESPMKAVSISEERSPMAQNAAQVLESFEHLPPLPEPPPLLPELVDKIRDTLPPQKPELKVKRVLRPRGIALTWNITKINPKCAPVESYHLFLCHENPSNKLIWKKIGEIKALPLPMACTLSQFLTYNKYYFTVQSKDIFGRYGPFCDIKSIPGFSETLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JK83</accession>
    <name>A0A9L0JK83_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">X-ray repair cross complementing 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">XRCC2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_014684506.1">
        <property type="nucleotide sequence ID" value="XM_014829020.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JK83"/>
    <dbReference type="Ensembl" id="ENSEAST00005076363.1">
        <property type="protein sequence ID" value="ENSEASP00005050382.1"/>
        <property type="gene ID" value="ENSEASG00005001714.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106823383"/>
    <dbReference type="KEGG" id="eai:106823383"/>
    <dbReference type="CTD" id="7516"/>
    <dbReference type="GeneTree" id="ENSGT00390000020445"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033063">
        <property type="term" value="C:Rad51B-Rad51C-Rad51D-XRCC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042148">
        <property type="term" value="P:DNA strand invasion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19490">
        <property type="entry name" value="XRCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001507">
        <property type="entry name" value="X-ray repair cross complementing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030547">
        <property type="entry name" value="XRCC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46644">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46644:SF2">
        <property type="entry name" value="DNA REPAIR PROTEIN XRCC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="1">
        <location>
            <begin position="16"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="283" mass="32284" checksum="346E303CF422B5D5" modified="2023-09-13" version="1">MCSAFHRAESGTELLARLEGRSSLKEIEPYLFANEDSPVLGDILEFHGPEGTGKTEMLYHLTARCILPKSEGGLEVEVLFIDTDYHFDMLRLVTILEHRLSQSCEEVVKRCLGRFFLVYCSSSIQLLLTLHSLEAMFCGHPSLRLLIVDSLSAFYWIDRVNGGESVNLQESTLKKCSQFLEKLVKEYRLVLFATTQSIMRKTSHLREGPSSAFSHLSDAGVDYRPYLCKAWQQVVKHRIFFSKDDSKTSNQFSLVSRHLKSNNFKKHFFIIGESGVEFCSYVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JMS7</accession>
    <name>A0A9L0JMS7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitochondrial import inner membrane translocase subunit TIM22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TIMM22</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 9">Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the TIM22 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014682399.1">
        <property type="nucleotide sequence ID" value="XM_014826913.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JMS7"/>
    <dbReference type="Ensembl" id="ENSEAST00005041871.1">
        <property type="protein sequence ID" value="ENSEASP00005050465.1"/>
        <property type="gene ID" value="ENSEASG00005034936.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106821989"/>
    <dbReference type="KEGG" id="eai:106821989"/>
    <dbReference type="CTD" id="29928"/>
    <dbReference type="GeneTree" id="ENSGT00390000016067"/>
    <dbReference type="OMA" id="VNPNMAD"/>
    <dbReference type="OrthoDB" id="75343at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039175">
        <property type="entry name" value="TIM22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="69"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="194" mass="20075" checksum="828E931243747D6E" modified="2023-09-13" version="1">MAATAPSAGGSAPEAAGSAEAPLQYSLLLQHLVGDKRQPRLLEPGSLGGIPSPAKSEEQKMIERAMESCAFKAALACVGGFVLGGAFGVFTAGIDTNVGFDPKDPYRTPTAREVLKDMGQRGMSYAKNFAIVGAMFSCTECLVESYRGKSDWKNSVISGCITGGAIGFRAGLKAGVIGCGGFAAFSAAIDYYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JMX8</accession>
    <name>A0A9L0JMX8_EQUAS</name>
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        <submittedName>
            <fullName evidence="5">Ras homolog family member G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RHOG</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JMX8"/>
    <dbReference type="Ensembl" id="ENSEAST00005065085.1">
        <property type="protein sequence ID" value="ENSEASP00005051337.1"/>
        <property type="gene ID" value="ENSEASG00005030666.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155158"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903078">
        <property type="term" value="P:positive regulation of protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016601">
        <property type="term" value="P:Rac protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150052">
        <property type="term" value="P:regulation of postsynapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900027">
        <property type="term" value="P:regulation of ruffle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001179">
        <property type="entry name" value="Rho family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
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    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
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        <location>
            <begin position="109"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
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        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
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    </evidence>
    <sequence length="139" mass="15378" checksum="BA50CDABE160FE49" modified="2023-09-13" version="1">MQSIKCVVVGDGAVGKTCLLICYTTNAFPKEYIPTVFDNYSAQSAVDGRTVNLNLWDTAGQEEYDRLRTLSYPQTNVFVICFSIASPPSYENVRHKWHPEVCHHCPDQVGTDPASSAPQGAVTAAGPRSRQQRRQRSVC</sequence>
</entry>
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    <accession>A0A9L0JPS2</accession>
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        <recommendedName>
            <fullName evidence="11">Histone H2B</fullName>
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    </protein>
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        <name evidence="15" type="primary">LOC123282516</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
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                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
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                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
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                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the histone H2B family.</text>
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        <property type="gene ID" value="ENSEASG00005035303.1"/>
    </dbReference>
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        <property type="component" value="Chromosome X"/>
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        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="HFD_H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Histone H2B"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="H2A/H2B/H3"/>
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    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
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        <property type="entry name" value="Histone_H2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055333">
        <property type="entry name" value="HISTONE_H2B_site"/>
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    <dbReference type="PANTHER" id="PTHR23428">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HISTONEH2B"/>
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        <property type="entry name" value="H2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
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        <property type="entry name" value="HISTONE_H2B"/>
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    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
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    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040393918" description="Histone H2B" evidence="13">
        <location>
            <begin position="20"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="14">
        <location>
            <begin position="59"/>
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        </location>
    </feature>
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        <location>
            <begin position="22"/>
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            <begin position="26"/>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005051145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="162" mass="18242" checksum="12ACBE1848F2E0A0" modified="2023-09-13" version="1">MRHHLSAAAIVLLLQNCCSTNSDMAEPGSEPSSEESLGTTEPTEADPKSLKQKQPRRRRRRCRRCRRCRCLDSFATYFPRVLRRVHEGLSLSQEAVSVLDSFAKDIFERIAGEATCLVRSTKRSTISCREIQTAVRLLLPGDLGKHAVAEGTKALIRYISRR</sequence>
</entry>
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    <accession>A0A9L0JTI7</accession>
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        <submittedName>
            <fullName evidence="8">Tubulin tyrosine ligase like 7</fullName>
        </submittedName>
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        <name evidence="8" type="primary">TTLL7</name>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the tubulin--tyrosine ligase family.</text>
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    <dbReference type="RefSeq" id="XP_014685578.1">
        <property type="nucleotide sequence ID" value="XM_014830092.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JTI7"/>
    <dbReference type="Ensembl" id="ENSEAST00005054965.1">
        <property type="protein sequence ID" value="ENSEASP00005056621.1"/>
        <property type="gene ID" value="ENSEASG00005014170.2"/>
    </dbReference>
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    <dbReference type="KEGG" id="eai:106824033"/>
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    <dbReference type="GeneTree" id="ENSGT00940000159078"/>
    <dbReference type="OMA" id="MNCCRRI"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043014">
        <property type="term" value="F:alpha-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048487">
        <property type="term" value="F:beta-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070740">
        <property type="term" value="F:tubulin-glutamic acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000009">
        <property type="entry name" value="tubulin polyglutamylase TTLL5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12241:SF147">
        <property type="entry name" value="TUBULIN POLYGLUTAMYLASE TTLL7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="3" id="KW-0493">Microtubule</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="516"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="648"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="413"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="548"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="564"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="592"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="655"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005056621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="907" mass="105267" checksum="96A1C92793AEC298" modified="2023-09-13" version="1">MPSLPQERVIQGPSPLDLNTELPYQSTMKRKIRKKKKKGTITANVAGTKFEIVRLVIDEMGFMKTPDEDETSNLIWCDSAVQQEKIAELQNYQRINHFPGMGEICRKDFLARNMTKMIKSRPLDYTFVPRTWIFPAEYTQFQNYMKELKKKRKQKTFIVKPANGAMGHGISLIRNGDKLPSQDHLIVQEYIEKPFLMEGYKFDLRIYILITSCDPLKIFLYHDGLVRMGTEKYIPPNESNLTQLYMHLTNYSVNKHNERFERDETENKGSKRSIKWFTEFLQANQHDVAKFWSDISELVVKTLIVAEPHVLHAYRMCRPGQPPGSESVCFEVLGFDILLDRKLKPWLLEINRAPSFGTDQKIDYDVKRGVLLNALKLLNIRTSDKRRNLAKQKAEAQRRLYGQNSIKRLLPGSSDWEQQRHQLERRKEELKERLAQVRKQILKEEHENRHMGNYRRIYPPEDKTLLEKYENLLAVAFQTFLSGRAALFQRELNNPLKRMKEEDILDLLEQCEIDDEKLMGKTAKPRGPKPLSSMPESTEMMKRQKYCSSESSYDSSSSSSESDGSGKEEYENKRGEKQVTYDLKQSSHYKLIQQSSSIRRSVSCPRSISSQSPSSADNRPFSAQQMIASRPTSGSRSHSLNRASSYMRHLPHGSDASSANSQMSESLRQLRTKEQEDHLTSQTLFVLKDMKIRFPGKSDAESLLLIEDIIDNWKYHKTKVASYWLIKLDSAKQRKVLDIVKTSVRTILPRIWKVPDVEEVNLYRIFNRVFNRLLWSHGQGLWNCFCDSGSSWESIFSKSPEVVTPLQLQCCQRLVELCKQCLLVVYKYTTDTRGSLSGVGTDWGNSRHIDWTGLMLTKGFPEENRLYRYLLPGSTQFIMRTPTYSLKYSSPGMTRSNVLFTSRYGHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JTL7</accession>
    <name>A0A9L0JTL7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106825928</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cell membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044605891.2">
        <property type="nucleotide sequence ID" value="XM_044749956.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JTL7"/>
    <dbReference type="Ensembl" id="ENSEAST00005038161.1">
        <property type="protein sequence ID" value="ENSEASP00005052490.1"/>
        <property type="gene ID" value="ENSEASG00005023924.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106825928"/>
    <dbReference type="KEGG" id="eai:106825928"/>
    <dbReference type="GeneTree" id="ENSGT00940000163528"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15939">
        <property type="entry name" value="7tmA_OR4A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000007">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050427">
        <property type="entry name" value="Olfactory_Receptors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48002">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="24"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="269"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="317" mass="35604" checksum="942FC7F79FDE40F0" modified="2023-09-13" version="1">MEYSNNVTEFVLLGLTQSPEGQKVLFVAFLLIYIVTVVGNLLIIVTIIASQSLGAPMYFFLAYLSFIDTVYSTAIAPKMIVDLVYEKKTISFHACMTPVFIDHLFAGAEVILLVVMAYDRYVAICKPLHYLIIMNQQVCVVMLLVTWTGGFLHSLVQFLCIYQLPFCGPNVIDNFVCDMYPLLKLACTNTYLVGLSMIANGGAICTVIFFILLVSYGVILYSLKTQGLEGKSKAFYTCASHMTVVILFFVPCIFLYARPNSTFPIDKSMTLVLTFITPMLNPLIYTLRNTEMKNAMRKLWSKNVTLVGRRLYPSCIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JUT0</accession>
    <name>A0A9L0JUT0_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">H1.7 linker histone</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">H1-7</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_014705525.1">
        <property type="nucleotide sequence ID" value="XM_014850039.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JUT0"/>
    <dbReference type="Ensembl" id="ENSEAST00005037573.2">
        <property type="protein sequence ID" value="ENSEASP00005053782.1"/>
        <property type="gene ID" value="ENSEASG00005023523.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106836831"/>
    <dbReference type="KEGG" id="eai:106836831"/>
    <dbReference type="CTD" id="341567"/>
    <dbReference type="GeneTree" id="ENSGT00730000111596"/>
    <dbReference type="OrthoDB" id="9451724at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005053782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="262" mass="28969" checksum="F940F400E8F9B85D" modified="2023-09-13" version="1">MAEAAEPAGESQGAEVQSQRPMEKVLAVPLRRGPSSVLKVSQLLLRAIAAHKGLTLAVLKRELGNAGYQVRRKCSRPSSEAPGTEVKGTFLRVSGSDAAGYFRVWKSPKPKRRPGRPRLEEGVRAPRRAAQVPRSPRRRHARPRAARKAREMWRRSARANVKARKTRPRAKEARGKAMGEGRGRSTKEDIRPRTREEKRPSSKAREEKKQDPEKSGKRTIQKPTSGKTDPTCSGQGKTHDARSARTKTSTKCEGPRNAAGNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JUZ1</accession>
    <name>A0A9L0JUZ1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Kin of IRRE-like protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Kin of irregular chiasm-like protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Nephrin-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">KIRREL1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Interacts with TJP1/ZO-1 and with NPHS2/podocin (via the C-terminus). Interacts with NPHS1/nephrin (via the Ig-like domains); this interaction is dependent on KIRREL1 glycosylation. Homodimer (via the Ig-like domains). Interacts when tyrosine-phosphorylated with GRB2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014836876.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JUZ1"/>
    <dbReference type="Ensembl" id="ENSEAST00005043143.1">
        <property type="protein sequence ID" value="ENSEASP00005057121.1"/>
        <property type="gene ID" value="ENSEASG00005003497.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106828430"/>
    <dbReference type="CTD" id="55243"/>
    <dbReference type="GeneTree" id="ENSGT00940000155795"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045217">
        <property type="term" value="P:cell-cell junction maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003094">
        <property type="term" value="P:glomerular filtration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05759">
        <property type="entry name" value="IgI_2_KIRREL3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05898">
        <property type="entry name" value="IgI_5_KIRREL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000232">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000077">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000094">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000170">
        <property type="entry name" value="Kirre like nephrin family adhesion molecule 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013162">
        <property type="entry name" value="CD80_C2-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051275">
        <property type="entry name" value="Cell_adhesion_signaling"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640:SF14">
        <property type="entry name" value="KIN OF IRRE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11640">
        <property type="entry name" value="NEPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08205">
        <property type="entry name" value="C2-set_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="7 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 17" id="KW-0732">Signal</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040164419" description="Kin of IRRE-like protein 1" evidence="17">
        <location>
            <begin position="17"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="511"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="21"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="120"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="223"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="308"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="18">
        <location>
            <begin position="408"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="670"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00074376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00076239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005057121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JWX8</accession>
    <name>A0A9L0JWX8_EQUAS</name>
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        <recommendedName>
            <fullName evidence="20">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">GRIK5</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15 20">Postsynaptic cell membrane</location>
            <topology evidence="15 20">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Presynaptic cell membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.</text>
    </comment>
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    <dbReference type="Ensembl" id="ENSEAST00005080538.1">
        <property type="protein sequence ID" value="ENSEASP00005054547.1"/>
        <property type="gene ID" value="ENSEASG00005006176.2"/>
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        <property type="component" value="Chromosome 26"/>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:hippocampal mossy fiber to CA3 synapse"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0032983">
        <property type="term" value="C:kainate selective glutamate receptor complex"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
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    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0042734">
        <property type="term" value="C:presynaptic membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0015277">
        <property type="term" value="F:kainate selective glutamate receptor activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099507">
        <property type="term" value="F:ligand-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904315">
        <property type="term" value="F:transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0060079">
        <property type="term" value="P:excitatory postsynaptic potential"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0031630">
        <property type="term" value="P:regulation of synaptic vesicle fusion to presynaptic active zone membrane"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0035249">
        <property type="term" value="P:synaptic transmission, glutamatergic"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06394">
        <property type="entry name" value="PBP1_iGluR_Kainate_KA1_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Glutamate receptor ionotropic, kainate 1"/>
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        <property type="entry name" value="glutamate receptor ionotropic, kainate 4"/>
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        <property type="entry name" value="Putative glutamate receptor ionotropic kainate 1"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Periplasmic binding protein-like II"/>
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        <property type="entry name" value="ANF_lig-bd_rcpt"/>
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        <property type="entry name" value="Glu/Gly-bd"/>
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        <property type="entry name" value="Peripla_BP_I"/>
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        <property type="entry name" value="Lig_chan"/>
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    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
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        <property type="entry name" value="NMDARECEPTOR"/>
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        <property type="entry name" value="PBPe"/>
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        <property type="entry name" value="Periplasmic binding protein-like I"/>
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    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14 20" id="KW-0407">Ion channel</keyword>
    <keyword evidence="7 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 20" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="8 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 20" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="9 20" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
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    <keyword evidence="3 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 20" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
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            <begin position="627"/>
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    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="705"/>
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    <feature type="transmembrane region" description="Helical" evidence="20">
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            <begin position="886"/>
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    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="22">
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            <begin position="498"/>
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    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="23">
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    <feature type="region of interest" description="Disordered" evidence="21">
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    <feature type="region of interest" description="Disordered" evidence="21">
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    <feature type="region of interest" description="Disordered" evidence="21">
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    <feature type="region of interest" description="Disordered" evidence="21">
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            <begin position="1111"/>
            <end position="1188"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="21">
        <location>
            <begin position="50"/>
            <end position="69"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="21">
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            <begin position="937"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="21">
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            <begin position="976"/>
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    <feature type="binding site" evidence="17">
        <location>
            <position position="583"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="588"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="755"/>
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        <ligand>
            <name>L-glutamate</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="756"/>
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        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="804"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
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        </ligand>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="18">
        <location>
            <position position="734"/>
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    </feature>
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        <location>
            <begin position="816"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00034107"/>
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        <source>
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        <source>
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    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="1188" mass="130803" checksum="566B8D57434C9A6F" modified="2023-09-13" version="1">MCWGAARERVSVSSPSRALGVVDPARAAGGQAGGGLGGEKERWRWQWRRAGGGSSNGGGGGQQQEGGEWGPRQLRPHEEGRKMPAELLLLLIVAFASPSCQVLSSLRMAAILDDQTVCGRGERLALALAREQINGIIEVPAKARVEVDIFELQRDSQYETTDTMCQILPKGVVSVLGPSSSPASASTVSHICGEKEIPHIKVGPEETPRLQYLRFASVSLYPSNEDVSLAVSRILKSFNYPSASLICAKAECLLRLEELVRGFLISKETLSVRMLDDSRDPTPLLKEIRDDKVSTIIIDANASISHLILRKASELGMTSAFYKYILTTMDFPILHLDGIVEDSSNILGFSMFNTSHPFYPEFVRSLNMSWRENCEASTYPGPALSAALMFDAVHVVVSAVRELNRSQEIGVKPLACTSANIWPHGTSLMNYLRMVEYDGLTGRVEFNSKGQRTNYTLRILEKSRQGHREIGVWYSNRTLAMNATTLDINLSQTLANKTLVVTTILENPYVMRRPNFQALSGNERFEGFCVDMLRELAELLRFRYRLRLVEDGLYGAPEPNGSWTGMVGELINRKADLAVAAFTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCVLFLAARLSPYEWYNPHPCLRARPHILENQYTLGNSLWFPVGGFMQQGSEIMPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPVESADDLADQTNIEYGTIHAGSTMTFFQNSRYQTYQRMWNYMQSKQPSVFVKSTEEGIARVLNSRYAFLLESTMNEYHRRLNCNLTQIGGLLDTKGYGIGMPLGSPFRDEITLAILQLQENNRLEILKRKWWEGSRCPKEEDHRAKGLGMENIGGIFVVLICGLIIAVFVAVMEFIWSTRRSAESEEVSVCQEMLQELRHAVSCRKTSRSRRRRRPGGPSRALLSLRAVREMRLSNGKLYSAGAGGDAGGAHGGPQRLLDDPGPPGGARPPAPPLHPRARLPGVPAHPGAAGLRGRRPPARPGRPRRGHQPAPAAAGPRRSPRAGRARVTTGGAERAPGLTAGTGPAPGPGRPHFPSGLGTGRTPQFCLRFPVKSNARLRGLPAPPSGLARGCRGRRTPPPTADKRSAGTGDSRARGLRGPFLEAESASGSALVQTAGPSPLTFSQDRRRLRQRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JZM8</accession>
    <name>A0A9L0JZM8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Filaggrin</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Metazoa</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
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                <person name="X."/>
                <person name="Zhao"/>
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                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="J."/>
                <person name="Pan"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
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                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
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                <person name="J."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <property type="term" value="F:structural molecule activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:establishment of skin barrier"/>
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        <property type="entry name" value="Filaggrin"/>
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    <dbReference type="InterPro" id="IPR052503">
        <property type="entry name" value="S100-fused_Epidermal_Struct"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22571:SF51">
        <property type="entry name" value="FILAGGRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22571">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03516">
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        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00487">
        <property type="entry name" value="FILAGGRIN"/>
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            <end position="692"/>
        </location>
    </feature>
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            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="114"/>
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            <begin position="139"/>
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    </feature>
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            <begin position="190"/>
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    </feature>
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        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="241"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="317"/>
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        </location>
    </feature>
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            <begin position="342"/>
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            <begin position="403"/>
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            <begin position="683"/>
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    </feature>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Guo"/>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Gao"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JZS8"/>
    <dbReference type="Ensembl" id="ENSEAST00005069971.1">
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        <property type="component" value="Chromosome 14"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20255">
        <property type="entry name" value="CASIMO1_SMIM22"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SIM_Modulators"/>
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        <property type="entry name" value="SMIM5/18/22"/>
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    <dbReference type="PANTHER" id="PTHR36982">
        <property type="entry name" value="CLCA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36982:SF3">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15831">
        <property type="entry name" value="SMIM5_18_22"/>
        <property type="match status" value="1"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="84"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005054600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="109" mass="11923" checksum="7DBE9AF87DC3CF82" modified="2023-09-13" version="1">MMPARPSCPRKRGGTQDPPAEMGLSVEDLETTAQEVLGKLRSRQPFQSKWDTAAFIILLVFISAVLLLLLLAGIHCCCCTCCSRGPPRSQKVSPKKESRRGVDNLALEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K0V3</accession>
    <name>A0A9L0K0V3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Zinc finger protein 75A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZNF75A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K0V3"/>
    <dbReference type="Ensembl" id="ENSEAST00005083329.1">
        <property type="protein sequence ID" value="ENSEASP00005058398.1"/>
        <property type="gene ID" value="ENSEASG00005037347.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162456"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000720">
        <property type="entry name" value="zinc finger protein 18 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001005">
        <property type="entry name" value="Zinc finger protein 75A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001261">
        <property type="entry name" value="Zinc finger protein 75a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF142">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF57.1-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="14">
        <location>
            <begin position="68"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="KRAB" evidence="14">
        <location>
            <begin position="141"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="308"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="336"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="364"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="392"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="249"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="258"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005058398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="415" mass="48444" checksum="D1F21B9C19A5811C" modified="2023-09-13" version="1">MGMCQDEEFWNTYQCQQEYLSKNSHKETEPVCKRAVPAHQILASPEQTNTKDWTVAPELVLPESQSLLTFEEVAMYFSQEEWELLDPTQKALYNDVMQENYETLISLAVLAHQILASPEQTNTKDWTVAPELVLPESQSLLTFEEVAMYFSQEEWELLDPTQKALYNDVMQENYETLISLALFVLPKPKVISCLEQGEEPWVQGSPEFKDCPRELPTGLKLKNDTENQQPICLSDLEIQEPEDIVSKKARAKVPQKTMGKENHGDTHRGGKWHQDLPQKERMKVSPWKEELLRLIDLHKKERAEDKPFKCQECRKSFRVSSDLIKHQRIHTEEKPYKCQQCDKRFRWSSDLNKHLTTHQGIKPYKCSSCGKSFSQSSNLHTHQRTHTGEKPFTCHECGKKFSQNYHLTKHRRTHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K4D0</accession>
    <name>A0A9L0K4D0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Arachidonate 5-lipoxygenase-activating protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">MK-886-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ALOX5AP</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotrimer. Interacts with LTC4S and ALOX5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Endoplasmic reticulum membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">The C-terminal part after residue 140 is mostly disordered.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the MAPEG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014687434.1">
        <property type="nucleotide sequence ID" value="XM_014831948.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K4D0"/>
    <dbReference type="Ensembl" id="ENSEAST00005065214.1">
        <property type="protein sequence ID" value="ENSEASP00005056195.1"/>
        <property type="gene ID" value="ENSEASG00005022395.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106825257"/>
    <dbReference type="KEGG" id="eai:106825257"/>
    <dbReference type="CTD" id="241"/>
    <dbReference type="GeneTree" id="ENSGT00940000158706"/>
    <dbReference type="OMA" id="NAPWHTQ"/>
    <dbReference type="OrthoDB" id="8659873at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
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        <property type="term" value="F:arachidonate binding"/>
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        <property type="term" value="F:enzyme activator activity"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:glutathione transferase activity"/>
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    <dbReference type="GO" id="GO:0004464">
        <property type="term" value="F:leukotriene-C4 synthase activity"/>
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        <property type="term" value="P:cellular response to calcium ion"/>
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        <property type="term" value="P:leukotriene production involved in inflammatory response"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0070207">
        <property type="term" value="P:protein homotrimerization"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="Leukotriene C4 synthase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Membrane associated eicosanoid/glutathione metabolism-like domain"/>
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    <dbReference type="InterPro" id="IPR001446">
        <property type="entry name" value="5_LipOase_AP"/>
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    <dbReference type="InterPro" id="IPR018295">
        <property type="entry name" value="FLAP/GST2/LTC4S_CS"/>
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        <property type="entry name" value="MAPEG"/>
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        <property type="entry name" value="MAPEG-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001129">
        <property type="entry name" value="Membr-assoc_MAPEG"/>
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    <dbReference type="PANTHER" id="PTHR10250:SF2">
        <property type="entry name" value="ARACHIDONATE 5-LIPOXYGENASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MICROSOMAL GLUTATHIONE S-TRANSFERASE"/>
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    <dbReference type="Pfam" id="PF01124">
        <property type="entry name" value="MAPEG"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="5LPOXGNASEAP"/>
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        <property type="entry name" value="MAPEG domain-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS01297">
        <property type="entry name" value="FLAP_GST2_LTC4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 11" id="KW-0434">Leukotriene biosynthesis</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="67"/>
            <end position="93"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="118"/>
            <end position="139"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010459"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022751"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU369123"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005056195.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="161" mass="18197" checksum="70BAF80A24FC7AE3" modified="2023-09-13" version="1">MDQETVGNVVLLAIVTLISVIQNGFFAHKVEHESKTQNGRSFQRTGTLAFERVYTANQNCVDAYPTFLVMLWSAGLLCSQVPAAFAGLMYLFVRQKYFVGYLGERTQSTPGYIFGKRIILFLFLMSLAGIFNYYLILFFGSDFENYIKTITTTISPLLLIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K554</accession>
    <name>A0A9L0K554_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Solute carrier organic anion transporter family member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SLCO6A1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the organo anion transporter (TC 2.A.60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K554"/>
    <dbReference type="Ensembl" id="ENSEAST00005061843.1">
        <property type="protein sequence ID" value="ENSEASP00005057487.1"/>
        <property type="gene ID" value="ENSEASG00005033983.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106831161"/>
    <dbReference type="KEGG" id="eai:106831161"/>
    <dbReference type="CTD" id="133482"/>
    <dbReference type="GeneTree" id="ENSGT01150000286985"/>
    <dbReference type="OrthoDB" id="5062115at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015347">
        <property type="term" value="F:sodium-independent organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043252">
        <property type="term" value="P:sodium-independent organic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000363">
        <property type="entry name" value="Solute carrier organic anion transporter family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000384">
        <property type="entry name" value="Solute carrier organic anion transporter family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002350">
        <property type="entry name" value="Kazal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036058">
        <property type="entry name" value="Kazal_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004156">
        <property type="entry name" value="OATP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00805">
        <property type="entry name" value="oat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388">
        <property type="entry name" value="ORGANIC ANION TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11388:SF95">
        <property type="entry name" value="SOLUTE CARRIER ORGANIC ANION TRANSPORTER FAMILY MEMBER 6A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07648">
        <property type="entry name" value="Kazal_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03137">
        <property type="entry name" value="OATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100895">
        <property type="entry name" value="Kazal-type serine protease inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51465">
        <property type="entry name" value="KAZAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="217"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="255"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="303"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="404"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="439"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="560"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="595"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="647"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="Kazal-like" evidence="12">
        <location>
            <begin position="478"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU362056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005057487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="701" mass="77317" checksum="EF557C70E0672216" modified="2023-09-13" version="1">MDKALVDAEPKATQAQRPESTTPRESRKAKKRPQRLLTVPASLIKLRSFSKEGNEVEAPLPARKKRRERKESSEGSCGWGCVVIPICQRFNNIYCFLIFYCILVTSQGIVFGLIDLSIDSFEKDNQLKTIESLMLSLGYDISSCLVVVFVAYYGGRGKILRWITASSFLIGFGSLLFAFPYFSGEKLPLNVETEDICQKMKIIRTCGKTSSSFKSKYVSFFILGQFVQGIAGIPLYVLGVVFLENNVAAHSSGTYLGILEASEIFGYALSYATGASVIKASKNSTSEENVEDSRDNLYWLKTWWIHFVFVSLIAWSALIPLSCFPNNVRGTAKIKAGKRKQPKDQEFGTSIKDLFATVWVLMKNSALVCLSLSRASDSLVMIGVSEFLPIYIENQFMLTRSDATTLAGLVLIPAGAVGHLLGGVIVSKLQMSYKGLMKFMIVTSAISLVLLAFIIFVRCDPIPFAGISEDYGGTGQLGNLTAPCNSHCGCSSTVYSAICGRDDIGYFSPCFAGCTNFKALGNQKAYYNCSCIKEGLTTSDGQGDFIDARRGTCDAKCYKLPLFIVFVFSTIVFAAFSGVPNILAIVRIVSDKQRAMALGMAYVILRIFGSIPGPLVFKIIRETSCIFRDVGRCGNLGSCWIYNRTKMAYLLVGVCFLCKVFTIFFTAIALRLQKHLPKENSEILPTLVKHLKVKKREKSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K6U7</accession>
    <name>A0A9L0K6U7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Proenkephalin-A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PENK</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Neuropeptide that competes with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Chromaffin granule lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the opioid neuropeptide precursor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K6U7"/>
    <dbReference type="Ensembl" id="ENSEAST00005040662.1">
        <property type="protein sequence ID" value="ENSEASP00005061196.1"/>
        <property type="gene ID" value="ENSEASG00005030368.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106841663"/>
    <dbReference type="KEGG" id="eai:106841663"/>
    <dbReference type="CTD" id="5179"/>
    <dbReference type="GeneTree" id="ENSGT00950000183149"/>
    <dbReference type="OrthoDB" id="9928775at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043679">
        <property type="term" value="C:axon terminus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034466">
        <property type="term" value="C:chromaffin granule lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001515">
        <property type="term" value="F:opioid peptide activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031628">
        <property type="term" value="F:opioid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002118">
        <property type="term" value="P:aggressive behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001662">
        <property type="term" value="P:behavioral fear response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038003">
        <property type="term" value="P:G protein-coupled opioid receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007626">
        <property type="term" value="P:locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009617">
        <property type="term" value="P:response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019233">
        <property type="term" value="P:sensory perception of pain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001964">
        <property type="term" value="P:startle response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019226">
        <property type="term" value="P:transmission of nerve impulse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006024">
        <property type="entry name" value="Opioid_neupept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000703">
        <property type="entry name" value="Proenkphlin_A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11438">
        <property type="entry name" value="PROENKEPHALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11438:SF3">
        <property type="entry name" value="PROENKEPHALIN-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01160">
        <property type="entry name" value="Opiods_neuropep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01028">
        <property type="entry name" value="OPIOIDPRCRSR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01029">
        <property type="entry name" value="PENKAPRCRSR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0257">Endorphin</keyword>
    <keyword evidence="10" id="KW-0527">Neuropeptide</keyword>
    <keyword evidence="7" id="KW-0555">Opioid peptide</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040487124" description="Proenkephalin-A" evidence="12">
        <location>
            <begin position="21"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="266" mass="30244" checksum="C4841E42554872EA" modified="2023-09-13" version="1">MARFLRLCTWLLALGPGLLATVRAECSQDCATCSYFLARPTDINPLACTLECEGKLTSLKTWETCKELLQLSKLELPQGGTSALRESSKQEESHLLAKKYGGFLKRYGGFMKKMDELYPVEPEEEANGGEILAKRYGGFMKKDAEEDAALGNSSDLLKELLATGDNRDSSHHQESSDDEEVSKRYGGFMRGLKRSPQLEDQAKELQKRYGGFMRRVGRPEWWMDYQKRYGGFLKRFADSLPSDEEGESYSKEVPEMEKRYGGFMRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K762</accession>
    <name>A0A9L0K762_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">MAPK regulated corepressor interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MCRIP1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Stress granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MCRIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K762"/>
    <dbReference type="Ensembl" id="ENSEAST00005079267.1">
        <property type="protein sequence ID" value="ENSEASP00005058212.1"/>
        <property type="gene ID" value="ENSEASG00005029020.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161850"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029428">
        <property type="entry name" value="MCRIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14799">
        <property type="entry name" value="FAM195"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="143"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="176"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="243"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005058212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="258" mass="28734" checksum="CC8821BFFDAA9E39" modified="2023-09-13" version="1">MARFPDEAGGGAHTQVCCSGRGPATLTREGGRQWCGAWRLCLGVFSGPPGPCLASSTALCCWQLFRSRSRFRNLIGCKYWAVESPKDSEYAREVESKKLHSIPKEHGMTTNRIIDSNSGIFHCHFSRQQEPHGYQGIQELSRGMQRPRGSGSGVQGPDTPAMTSSPVSRVVYNGKRNSSPRSPPNSSEIFTPAHEENVRFIYEAWQGVERDLRSQMSGSERGLVEEYVEKVPNPSLKTFKPIDLSDLKRRNTQDTKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KB40</accession>
    <name>A0A9L0KB40_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">T-complex protein 1 subunit eta</fullName>
            <shortName evidence="14">TCP-1-eta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 14">CCT-eta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CCT7</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the chaperonin-containing T-complex (TRiC), a hexadecamer composed of two identical back-to-back stacked rings enclosing a protein folding chamber. Each ring is made up of eight different subunits: TCP1/CCT1, CCT2, CCT3, CCT4, CCT5, CCT6A/CCT6, CCT7, CCT8. Interacts with PACRG. Interacts with DLEC1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Heterooligomeric complex that forms two stacked rings.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014692119.1">
        <property type="nucleotide sequence ID" value="XM_014836633.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0KB40"/>
    <dbReference type="Ensembl" id="ENSEAST00005058832.1">
        <property type="protein sequence ID" value="ENSEASP00005059632.1"/>
        <property type="gene ID" value="ENSEASG00005015184.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106828291"/>
    <dbReference type="KEGG" id="eai:106828291"/>
    <dbReference type="CTD" id="10574"/>
    <dbReference type="GeneTree" id="ENSGT00550000074832"/>
    <dbReference type="OMA" id="HRKGNTW"/>
    <dbReference type="OrthoDB" id="1935484at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044297">
        <property type="term" value="C:cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032212">
        <property type="term" value="P:positive regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03340">
        <property type="entry name" value="TCP1_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000045">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000022">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000049">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000006">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012720">
        <property type="entry name" value="Chap_CCT_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02345">
        <property type="entry name" value="chap_CCT_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="524"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00093427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00093487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU365042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005059632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="543" mass="59430" checksum="E0D4842FB46AA230" modified="2023-09-13" version="1">MMPTPVILLKEGTDSSQGIPQLVSNITACQVIAEAVRTTLGPRGMDKLIVDGRGKATISNDGATILKLLDVVHPAAKTLVDIAKSQDAEVGDGTTSVTLLAAEFLKQVKPYVEEGLHPQIIIRAFRTATQLAVNKIKEIAVTVKKEDKVEQRKLLEKCAMTALSSKLISQQKAFFAKMVVDAVIMLDDLLQLKMIGIKKVQGGALEESQLVAGVAFKKTFSYAGFEMQPKKYNNPMIALLNVELELKAEKDNAEIRVHTVEDYQAIVDAEWNILYDKLERIHHSGAKVVLSKLPIGDVATQYFADRDMFCAGRVPEEDLKRTMMACGGSIQTSVNALSADVLGHCQVFEETQIGGERYNFFTGCPKAKTCTIILRGGAEQFMEETERSLHDAIMIVRRAIKNDSVVAGGGAIEMELSKYLRDYSRTIPGKQQLLIGAYAKALEIIPRQLCDNAGFDATNILNKLRARHAQGGMWYGVDINNEDIADNFEAFVWEPAMVRINALTAASEAACLIVSVDETIKNPRSTVEAPPAAGRGRGRGRPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KBU4</accession>
    <name>A0A9L0KBU4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Taxilin gamma</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TXLNG</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the taxilin family.</text>
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    <dbReference type="RefSeq" id="XP_044620262.1">
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        <property type="protein sequence ID" value="ENSEASP00005062288.1"/>
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        <property type="component" value="Chromosome Y"/>
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        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="P:regulation of cell cycle"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Taxilin_fam"/>
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        <property type="entry name" value="GAMMA-TAXILIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TAXILIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF09728">
        <property type="entry name" value="Taxilin"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="138"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="188"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="358"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="456"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005062288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="548" mass="62539" checksum="FBE5DDC0F85A4CEE" modified="2023-09-13" version="1">MRRLDVGVQGRGARPCEVPCPLSGRLFGCCRHLMATRVEEAARGRGGGAEEAIEAGRGGRRRSPRQKFVIGTMEEAGMCGLGVKADMLCNSQSNDILQRQDSNCGGTRNKHSLEDEGSDFITKNRSLVSPACCTQELREEIPGREARTDPPDGQQDSECNRNKDKTLGKEVLLLMQALNTLSTPEEKLAALCKKYADLLEESRNVQKQMKILQKKQAQIVKEKVHLQSEHSKAILARSKLESLCRELQRHNKTLKEENMQQAREEEERRKEATAHFQITLNEIQAQLEQHDIHNAKLRQENIELGEKLKKLIEQYALREEHIDKVFKHKELQQQLVDAKLQQTTQLIKEADEKHQREREFLLKEATESRHKYEQMKQQEVQLKQQLSLYMDKFEEFQTTMAKSNELFTTFRQEMEKMTKKIKKLEKETIIWRTKWENNNKALLQMAEEKTVRDKEYKAFQIKLERLEKLCRALQTERNELNEKVEVLKEQASGRVAGADLAPPGIQPCAALASPGELNTASARALRAPLEAVLQAASLGTGPGIESVD</sequence>
</entry>
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    <accession>A0A9L0KHM5</accession>
    <name>A0A9L0KHM5_EQUAS</name>
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        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSEAST00005072492.1">
        <property type="protein sequence ID" value="ENSEASP00005062307.1"/>
        <property type="gene ID" value="ENSEASG00005037281.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154455"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
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        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:adaptive immune response"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367:SF43">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 6-4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040303395" description="Ig-like domain-containing protein" evidence="6">
        <location>
            <begin position="21"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="5"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005062307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="126" mass="13508" checksum="BE7DB42145D76FD9" modified="2023-09-13" version="1">MNSAPGLVTVVLLMLGQTHGESVTQMEGQVTLSEGASLTVNCTYSATGYPILYWYVQYLGEGPQLLLRAAKANEKGKNKDFEATYNTETTSFHLEKASVQESDSAMYYCAVSDTVTGAAGGAEHKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KII2</accession>
    <name>A0A9L0KII2_EQUAS</name>
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        <recommendedName>
            <fullName evidence="4">Metallothionein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
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                <person name="J."/>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
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                <person name="S."/>
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                <person name="Wang"/>
                <person name="X."/>
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                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Metallothioneins have a high content of cysteine residues that bind various heavy metals.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the metallothionein superfamily. Type 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KII2"/>
    <dbReference type="Ensembl" id="ENSEAST00005064714.1">
        <property type="protein sequence ID" value="ENSEASP00005062668.1"/>
        <property type="gene ID" value="ENSEASG00005036572.1"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Metallothionein Isoform II"/>
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        <property type="entry name" value="Metalthion_dom_sf"/>
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        <property type="entry name" value="Metalthion_vert"/>
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        <property type="entry name" value="METALLOTHIONEIN"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00007283"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022851"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000621"/>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005062668.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="83" mass="8747" checksum="956DFEB9812E4098" modified="2023-09-13" version="1">MLKARAEEFEIDSAISGLSHHFISMDPNCSCTTSESCICTSCKKSCSSCCPVGCAKCAQGCVCKGYRTSAAAVPDVWVSLLPM</sequence>
</entry>
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    <accession>A0A9L0KK02</accession>
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        <recommendedName>
            <fullName evidence="3">Transmembrane protein 19</fullName>
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    </protein>
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            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM19 family.</text>
    </comment>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353:SF5">
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="311"/>
            <end position="335"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="377"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="516"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="553"/>
            <end position="573"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="611"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="41"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="94"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
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                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="G."/>
                <person name="Liu"/>
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                <person name="Liu"/>
                <person name="W."/>
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                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="C."/>
                <person name="Jin"/>
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                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="component" value="Chromosome X"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Melanoma-associated antigen C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1210:FF:000001">
        <property type="entry name" value="melanoma-associated antigen D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1200">
        <property type="entry name" value="MAGE homology domain, winged helix WH1 motif"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1210">
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    </dbReference>
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        <property type="entry name" value="MAGE"/>
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    <dbReference type="InterPro" id="IPR021072">
        <property type="entry name" value="MAGE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041898">
        <property type="entry name" value="MAGE_WH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041899">
        <property type="entry name" value="MAGE_WH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002190">
        <property type="entry name" value="MHD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11736:SF67">
        <property type="entry name" value="MELANOMA-ASSOCIATED ANTIGEN B6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11736">
        <property type="entry name" value="MELANOMA-ASSOCIATED ANTIGEN MAGE ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01454">
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MAGE" evidence="2">
        <location>
            <begin position="107"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="33"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000395.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="314" mass="35014" checksum="7FCFF9260C7B5645" modified="2023-09-13" version="2">MPRGQKSKLRAREKRRQARGETQSLRGAQATGAAAEESPSSPVSWGTPPSSPAAGTPQEPQGAPVTSSPDAGVSCPRSDEGAKSQDEQNASTSQAATSTQSTHRDPLTRKVSMLVQFLLEKYKMKEPIMQAALLKIVNKKYKEHFPEILRRSSEHLELVFGLELKEVDPSNHVYILVSKLGLLGNESLSSDKGLPKTSLLMTLLGVIFMKGNRATEEEIWEFLNVLGVYAGRKHLIFGEPRKLITKDFVQEKYLEYRQVPNSDPPCYEFLWGPRAYAETNKMKVLEVLAKINDTVPTAFPNLYEEALRDEEERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KU37</accession>
    <name>A0A8C4KU37_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Malonate--CoA ligase ACSF3, mitochondrial</fullName>
            <ecNumber evidence="8">6.2.1.76</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Acyl-CoA synthetase family member 3</fullName>
        </alternativeName>
    </protein>
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        <name evidence="13" type="primary">ACSF3</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the initial reaction in intramitochondrial fatty acid synthesis, by activating malonate and methylmalonate, but not acetate, into their respective CoA thioester. May have some preference toward very-long-chain substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>malonate + ATP + CoA = malonyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:32139"/>
            <dbReference type="ChEBI" id="CHEBI:15792"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.76"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:32140"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>tetracosanoate + ATP + CoA = tetracosanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:33639"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:31014"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:33640"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.76" evidence="8"/>
    <dbReference type="RefSeq" id="XP_014692059.1">
        <property type="nucleotide sequence ID" value="XM_014836573.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014692060.1">
        <property type="nucleotide sequence ID" value="XM_014836574.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KU37"/>
    <dbReference type="Ensembl" id="ENSEAST00005001093.2">
        <property type="protein sequence ID" value="ENSEASP00005000947.1"/>
        <property type="gene ID" value="ENSEASG00005000813.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106828244"/>
    <dbReference type="KEGG" id="eai:106828244"/>
    <dbReference type="CTD" id="197322"/>
    <dbReference type="GeneTree" id="ENSGT00940000157000"/>
    <dbReference type="OMA" id="KGKWFKT"/>
    <dbReference type="OrthoDB" id="2962993at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090409">
        <property type="term" value="F:malonyl-CoA synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031957">
        <property type="term" value="F:very long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090410">
        <property type="term" value="P:malonate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd05941">
        <property type="entry name" value="MCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000031">
        <property type="entry name" value="Acyl-CoA synthetase family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000030">
        <property type="entry name" value="Acyl-CoA synthetase family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201:SF8">
        <property type="entry name" value="ACYL-COA SYNTHETASE FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="11">
        <location>
            <begin position="49"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="12">
        <location>
            <begin position="487"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00078916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="585" mass="65682" checksum="36C41CB0A3270479" modified="2022-01-19" version="1">MPPHVVMSFRRLVWALASHRPAPLRHEGIRPLHAAPAACSDRSAPVFTRALAFGDRVALVDQHGHHTYKDLYHRSLCLSREICRLRECAGGDLQEERISFMCSNDVSYVVAQWASWMSGGIAVPLYRKHPQAELEYFIQDSRSSVVLAGQEYVELLSPVVRKLGVPLLPLTPTVYDGVAEEAGDGQLPERDWRDRGAMIIYTSGTTGRPKGVLSTHYNIRAVVTGLVHKWGWTKDDVILHVLPLHHVHGVVNKLLCPLWVGATCVMLPNFNAQLVWEKFLSSETPRINVFMAVPTIYTKLMDYYDKHFTQPHVQDFVRAVCEEKIRLMVSGSAALPLPVLEKWKNITGHTLLERYGMTEIGMALSNPLTAARLPGSVGTPLPGVEVRIVSENPQKEGYPYILHAEGNEKETKVTPGFKEKEGELLVRGPSVFREYWDKPEETKNAFTSDGWFKTGDTAVFKDGRYWIRGRTSVDIIKSGGYKISALEVERLLLAHPSITDVAVIGVPDMTWGQRVTAVVTLQEGHSLSHRELKEWARDVMAPYVVPSELLLVEEIPRNQMGKVNKRDLVRHFYPCNQGAPEAGRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KZA4</accession>
    <name>A0A8C4KZA4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106830125</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KZA4"/>
    <dbReference type="Ensembl" id="ENSEAST00005001450.2">
        <property type="protein sequence ID" value="ENSEASP00005001281.1"/>
        <property type="gene ID" value="ENSEASG00005001071.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286912"/>
    <dbReference type="OMA" id="PYCRTRT"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15951">
        <property type="entry name" value="7tmA_OR52R_52L-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000006">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF39">
        <property type="entry name" value="OLFACTORY RECEPTOR FAMILY 52 SUBFAMILY X MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="62"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="43"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="314" mass="34933" checksum="B77A5034CDFBBCD0" modified="2022-01-19" version="1">MSLSNDTNLHPSTFVLLGIPGMEHLHVWIGIPFCSMYLVAVVGNVTILAVVRAERSLHEPMFLFLCMLSVTDLVLSTSTLPRMLCLFWFGAHDIAFDACLAQMFFIHSFTTMESGFFLAMAVDRYVAICDPLHHTTILTHARITIMGIIVVTRGVAFFSPHPILLKQLPYCRTRIIAHTYCEFMAVVKLACVDTGATTRYSLSVASIIGSCDAILIAVSYAFILRSVLRLPSREASFKALGTCGSHVCVILVFYSTAGFSIFTHRFGKNVPAHIHIFIANMYLLVPPFLNPIVYGVRTKKIREHVLRALMVKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KZP9</accession>
    <name>A0A8C4KZP9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome b-c1 complex subunit 2, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Complex III subunit 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Core protein II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Ubiquinol-cytochrome-c reductase complex core protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106841746</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="15">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 11 subunits. The complex is composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein UQCRFS1, 2 core protein subunits UQCRC1/QCR1 and UQCRC2/QCR2, and 6 low-molecular weight protein subunits UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and subunit 9, the cleavage product of Rieske protein UQCRFS1. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and cytochrome c oxidase (complex IV, CIV), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)). Interacts with RAB5IF. Interacts with STMP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the peptidase M16 family. UQCRC2/QCR2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KZP9"/>
    <dbReference type="Ensembl" id="ENSEAST00005004040.2">
        <property type="protein sequence ID" value="ENSEASP00005003681.2"/>
        <property type="gene ID" value="ENSEASG00005002601.2"/>
    </dbReference>
    <dbReference type="KEGG" id="eai:106841746"/>
    <dbReference type="GeneTree" id="ENSGT00940000154915"/>
    <dbReference type="OrthoDB" id="6369905at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000018">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000026">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF226">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="16">
        <location>
            <begin position="49"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="17">
        <location>
            <begin position="199"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00041778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00064731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003681.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="453" mass="48133" checksum="EE90014A79A9FDB4" modified="2023-09-13" version="2">MKLLSRAGSLSRFYSLKVAPKVKATAAPAGVPPHPQDLEFTKLPNGLVIASLENYAPASRIGLFIKAGSRYEDSNNLGTSHLLRLASSLTTKGASSFKITRGIEAVGGTLSVTSTRENMAYTVECLRDDIDILMEFLLNVTTSPEFRRWEVAALQSQLRIDKAVAFQNPQAHVIENLHAAAYRNALANSLYCPDYRIGKVTPDELHHYVQNHFTSARMALVGLGVSHPVLKQVAEQFLNMRGGLGLSGAKARYRGGEIREQNGDSLVHAALVAESAAIGSAEANAFSVLQHVLGAGPHVKRGSNATNSLYQAVAKGTNQPFDVSAFNASYSDSGLFGFYTISQSAAAGDVIKAAYNQVKKIAQGNLSSADVQAAKNKLKAGYLMSVESSEGFLDEVGSQALAAGSYTPPSTVLQQIDSVADADIINAAKKFVSGQKSMAASGNLGHTPFVDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KZR1</accession>
    <name>A0A8C4KZR1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106836041</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KZR1"/>
    <dbReference type="Ensembl" id="ENSEAST00005003292.2">
        <property type="protein sequence ID" value="ENSEASP00005003013.2"/>
        <property type="gene ID" value="ENSEASG00005002311.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106836041"/>
    <dbReference type="KEGG" id="eai:106836041"/>
    <dbReference type="GeneTree" id="ENSGT01040000240383"/>
    <dbReference type="OrthoDB" id="6151005at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000004">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="42"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="145"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="211"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="56"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003013.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="324" mass="36158" checksum="3A6BD32B97B9B5EB" modified="2023-09-13" version="2">MGFFSPILQGPNLRKMAAENHSTVTEFILGGLTNRPELQLPLFLLFLGIYSVTMIGNLGMFTLICLNAQLHTPMYYFLSNLSLVDLCYSSVITPKMLVNFVSEKNVISYAGCMSQLYFFLVFVIAECYMLTVMAYDRYVAICSPLLYNIIVSHQVCTLLVTAVYAIGFIGSAIETGLMLKLSYCELFISHYFCDILPLMKLSCSSTYDIEMVVFFLAGFDIIVTSLTVLVSYVFILSSILHISTTEGRAKAFSTCSSHVIAVGIFYGSTAFMYLKPSTASSLAQENVASVFYTTIIPMLNPLIYSLRNKEVKAAVQKTLRRKMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L2M6</accession>
    <name>A0A8C4L2M6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ubiquitin carboxyl-terminal hydrolase 34</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Deubiquitinating enzyme 34</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ubiquitin thioesterase 34</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Ubiquitin-specific-processing protease 34</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">USP34</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Ubiquitin hydrolase that can remove conjugated ubiquitin from AXIN1 and AXIN2, thereby acting as a regulator of Wnt signaling pathway. Acts as an activator of the Wnt signaling pathway downstream of the beta-catenin destruction complex by deubiquitinating and stabilizing AXIN1 and AXIN2, leading to promote nuclear accumulation of AXIN1 and AXIN2 and positively regulate beta-catenin (CTNBB1)-mediated transcription. Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with AXIN1 and AXIN2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="Ensembl" id="ENSEAST00005003735.2">
        <property type="protein sequence ID" value="ENSEASP00005003406.2"/>
        <property type="gene ID" value="ENSEASG00005002220.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158659"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02659">
        <property type="entry name" value="peptidase_C19C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000014">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056850">
        <property type="entry name" value="ARM_UBP34_24_USP9X_Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021905">
        <property type="entry name" value="DUF3517"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF827">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25010">
        <property type="entry name" value="ARM_UBP24_USP9X-Y"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12030">
        <property type="entry name" value="DUF3517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="15">
        <location>
            <begin position="1895"/>
            <end position="2240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="112"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="503"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="550"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1459"/>
            <end position="1478"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="3333"/>
            <end position="3446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="115"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="510"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="525"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="560"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="575"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="591"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="610"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="659"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="1467"/>
            <end position="1477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="3337"/>
            <end position="3348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00082193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003406.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="3543" mass="403499" checksum="456E6E4788494E04" modified="2023-09-13" version="2">MCENCADLVEVLNEISDIEGGDGLQLRKEHTLKIFAYINSWTQRQCLCCFKEYKHLEIFNQVVCALINLVIAQVQVLRDQLCKHCTTVNIDSTWQDENNQVEEPLNIERQCNEGSTERQKSIEKKSNSTRICNRTEEESSKSSDPFSLWSTDEKEKLLLCVAKIFQIQFPLYTAYKHNTHPTIEDISTQESNILGAFCDMNDVEVPLHLLRYVCLFCGKNGLSLMKDCFEYGTPETLPFLIAHAFITVVSNIRIWLHIPAVMQHIIPFRTYVIRYLCKLSDQELRQSAARNMADLMWSTVKEPLDTTLCFDKESLDLAFKYFMSPTLTMRLAGLSQITNQLHTFNDVCNNESLVSDTETSIAKELADWLISNNVVEHIFGPNLHIEIIKQCQVILNFLAAEGRLSTQHIDCIWAAAQLKHCSRYIHDLFPSLIKNLDPVPLRHLLNLVSALEPSVHTEQTLYLASMLIKALWNNALAAKAQLSKQSSFASLLNTNLPIGNKKEEEELRRAAPSPWSPAASPQSSDNSDTHQSGGSDIEMDEQLINRTKHVQQRLSDTEESMQGSSDETANSAEDGSSGPGSSSGHSDGSSNEVNSSHASQSAGSPGSEVQSEDIADIEALKEEDEDDDHGHNPPKSSCGTDLRNRKLESQAGICLGDSQGPSERSGTSNGTGKDLVFNTESLPSVDNRIRMLDACSHSEDPENDISGEMNAAHIAQASQESCITRTGDFLGETIGNELFNCRQFIGPQHHHHHHHHHHHHDGHMVDDMLSADDVSCSSSQVSAKSEKNMADFDGEESGCEEELVQINSHAELTSHLQQHLPNLASIYHEHLSQGPAVHKHQFNSNAVTDINLDNVCKKGNTLLWDIVQDDDAVNLSEGLINEAEKLLCSLVCWFTDRQIRMRFIEGCLENLGNNRSVVISLRLLPKLFGTFQQFGSSYDTHWITMWAEKELNMMKLFFDNLVYYIQAVREGRQKHALYSHSAEVQVRLQFLTCVFSTLGSPDHFRLSLEQVDILWHCLVEDSECYDDALHWFLNQVRSKDQHAMGMETYKHLFLEKMPQLKPETISMTGLNLFQHLCNLARLATSAYDGGSNSELCGMDQFWGIALRAQSGDVSRAAIQYINSYYINGKTGLEKEQEFISKCMESLMIASSSLEQESHSSLTVIERGLLMLKTHLEAFRRRFAYHLRQWQIEGTGISSHLKALSDKQSLPLRVVCQPAGLPDKMTIEMYPSDQVADLRAEVTHWYENLQKEQINQQAQLQEFGQSSRKGEFPGGLMGPVRMISSGHELTTDYDEKALHELGFKDMQMVFVSLGAPRRERKGEGVQLPASCLPPPQKDNIPMLLLLQEPHLTTLFDLLEMLASFKPPSGKVAVEDSESLRCEELHLHAENLSRRVWELLMLLPTCPNMLMAFQNISEEQSNDGLNWKELLKIKSAHKLLYALEIIEALGKPNRRIRRESTGSYSDLYPDSDDSSEDQVENSKNSWSCKFVAAGGLQQLLEIFNSGILEPKEQESWTVWQLDCLACLLKLICQFAVDPSDLDLAYHDVFAWSGIAESHRKRTWPGKSRKAAGDHAKGLHIPRLTEVFLVLVQGTSLIQRLMSVAYTYDNLAPRVLKAQSDHRSRHEVSHYSMWLLVSWAHCCSLVKSSLADSDHLQDWLRKLTLLIPETAVRHESCNGLYKLSLSGLDGGDSINRSFLLLAASTLLKFLPDAQALKPIRIDDYEEEPMLKPGCKEYFWLLCKLVDNIHIKDASQTTLLDLDALARHLADCIRSREILDHQDGNIEDDGLTGLLRLATSVIKHKPPFKFSREGQEFLRDIFNLLFLLPSLKDRQQPKCKSHSSRAAAYDLLVEMVKGSVENYRLIHNWVMAQHMQSAHAPYKWDYWPHEDVRAECRFVGLTNLGATCYLASTIQQLYMIPEARQAVFTAKYSEDMKHKTTLLELQKMFTYLMESECKAYNPRPFCKTYTMDKQPLNTGEQKDMTEFFTDLITKIEEMSPELKNTVKSLFGGVITNNVVSLDCEHVSQTAEEFYTVRCQVADMKNIYESLDEVTIKDTLEGDNMYTCSHCGKKVRAEKRACFKKLPRILSFNTMRYTFNMVTMMKEKVNTHFSFPLRLDMTPYTEDFLMGKSDRKEGFKEVSDHSKDTESYEYDLIGVTVHTGTADGGHYYSFIRDIVNPLAYKNNKWYLFNDAEVKPFDSAQLASECFGGEMTTKTYDSVTDKFMDFSFEKTHSAYMLFYKRMEPEEENGKDYKFDVSSELLEWIWHDNMQFLQDKNIFEHTYFGFMWQLCSCIPSTLPDPKAVSLMTAKLSTSFVLETFIHSKEKPTMLQWIELLTKQFNNSQAACEWFLDRMADDDWWPMQILIKCPNQIVRQMFQRLCIHVIQRLRPVHAHLYLQPGMEDGSDDMDASVEDIGGRSCVTRFVRTLLLIMEHGVKPHSKHLTEYFAFLYEFAKMGEEESQFLLSLQAISTMVHFYMGTKGPENPQVEVLSEEEGEEEEEEEDILSLAEEKYRPAALEKMIALVALLVEQSRSERHLTLSQTDMAALTGGKGFPFLFQHIRDGINIRQTCNLIFSLCRYNNRLAEHIVSMLFTSIAKLTPEAANPFFKLLTMLMEFAGGPPGMPPFASYILQRIWEVIEYNPSQCLDWLAVQTPRNKLAHSWVLQNMENWVERFLLAHNYPRVRTSAAYLLVSLIPSNSFRQMFRSTRSLHIPTRDLPLSPDTTVVLHQVYNVLLGLLSRAKLYVDAAVHGTTKLVPYFSFMTYCLISKTEKLMFSTYFMDLWNLFQPKLSEPAIATNHNKQALLSFWYNVCADCPENIRLIVQNPVVTKNIAFNYILADHDDQDVVLFNRGMLPAYYGILRLCCEQSPAFTRQLASHQNIQWAFKNLTPHASQYPGAVEELFNLMQLFIAQRPDMREEELEDIKQFKKTTISCYLRCLDGRSCWTTLISAFRILLESDEDRLLVVFNRGLILMTESFNTLHMMYHEATACHVTGDLVELLSIFLSVLKSTRPYLQRKDVKQALIQWQERIEFAHKLLTLLNSYSPPELRNACIDVLKELVLLSPHDFLHTLVPFLQHNHCTYHHSNIPMSLGPYFPCRENIKLIGGKSNIRPPRPELNMCLLPTMVETSKGKDDVYDRMLLDYFFSYHQFIHLLCRVAINCEKFTETLVKLSVLVAYEGLPLHLALFPKLWTELCQTQSAMSKNCIKLLCEDPVFAEYIKCILMDERTFLNNNIVYTFMTHFLLKVQGQVFSEANCANLISTLITNLINQYQNLQSDFTNRIEISKASASLNGDLRALALLLSVHTPKQLNPALIPTLQELLSKCRTCLQQRNSLQEQEAKERKTKDDEGATPVKRRRVSSDEEHTVDSCISDLKTETREALTPTSTSDNETRDSSIIDPGTEQDLPSPENSSVKEYRMEVPSSFSEDIRSQHAEQSNNGKFEDCKEFKDLPCSKDPNLAEEESEFPSTSISAVLSDLADLRSCDGPALPSQDPEAALSLSCGHSRGLFSHMQQHDILDTLCRTIESTIHVVTRISGKGNQAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L514</accession>
    <name>A0A8C4L514_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Microtubule crosslinking factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MTCL1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005004261.2">
        <property type="protein sequence ID" value="ENSEASP00005003881.2"/>
        <property type="gene ID" value="ENSEASG00005002944.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000175881"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016327">
        <property type="term" value="C:apicolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016328">
        <property type="term" value="C:lateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097427">
        <property type="term" value="C:microtubule bundle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001578">
        <property type="term" value="P:microtubule bundle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049885">
        <property type="entry name" value="MTCL1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027882">
        <property type="entry name" value="SOGA1/2-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027881">
        <property type="entry name" value="SOGA_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15742">
        <property type="entry name" value="GIRDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15742:SF3">
        <property type="entry name" value="MICROTUBULE CROSS-LINKING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11365">
        <property type="entry name" value="SOGA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14818">
        <property type="entry name" value="SOGA1-2-like_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SOGA coiled-coil" evidence="7">
        <location>
            <begin position="502"/>
            <end position="596"/>
        </location>
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    <feature type="domain" description="SOGA coiled-coil" evidence="7">
        <location>
            <begin position="631"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="domain" description="SOGA 1/2-like coiled-coil" evidence="8">
        <location>
            <begin position="1267"/>
            <end position="1320"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
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            <begin position="194"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="735"/>
            <end position="797"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1096"/>
            <end position="1143"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1385"/>
            <end position="1419"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1518"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
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            <begin position="1563"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1699"/>
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    <feature type="region of interest" description="Disordered" evidence="6">
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            <begin position="1747"/>
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        <location>
            <begin position="1818"/>
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        <location>
            <begin position="1905"/>
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            <begin position="334"/>
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    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="433"/>
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    <feature type="coiled-coil region" evidence="5">
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            <begin position="517"/>
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            <begin position="572"/>
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    <feature type="coiled-coil region" evidence="5">
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            <begin position="1288"/>
            <end position="1315"/>
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        <location>
            <begin position="1"/>
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            <begin position="15"/>
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            <begin position="282"/>
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            <begin position="1842"/>
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            <dbReference type="ARBA" id="ARBA00004370"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF11365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14818"/>
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    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003881.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="2012" mass="221862" checksum="F11785414A0AD3B8" modified="2023-09-13" version="2">MAPRAAARRTRSRSRPASTTATITPTRWPSGGGCTVHPRPPDRSSRTCTAGPPRPAQPRPPPPRAEPRRPPPRARPAARGQGAALAGAPGRARPPLPAQRRRPGREGQAAAGARGPGQRAAPRPLRAPGGQRARRPAEPLPRAGKPLPPGAEPPPTAAKGRKAKRGSGTLPARAAGLPTPAASVPSVILAVTSAAGSPAPGSRISHTDSSSDLSDCPSEPLSDEQRLLPAASSDAESGTGSSDREPLRGAPTASPAARGAPPGSPEPSALLAVPTASGVCLGGRSSPGGVPAASSAPGLAEDAGERVLLEQTLPGNPKEPGSGDQPQLVPAVEEEELLREMEELRSENDYLKDELDELRAEMEEMRDSYLEEDVYQLQELRRELDRANKNCRILQYRLRKAEQKSLKVAETGQVDGELIRSLEQDLKVAKDVSVRLHHELETVEEKRAKAEDENEVLRQQMIEVEISKQTLQNELERLKESSLKRRGGRETYKEKKAFNQDDSADLKCQLQFAKEEGSLMRKKMAKLGKEKDELEQELQKYKALYGDVDSPLPTGEAGGPPSTREAELKLRLKLVEEEANILGRKIVELEVENRGLKAEMEDLRGQYEREGPGRDHIPSIPTSPFGDSLESSTELRRHLQFVEEEAELLRRSISEIEDHNRQLTHELSKFKFELRPEPGWLAEKGAGSGVPLQEELKSARLQINELSGKVLKLQYENRVLLSNVQRYDLAAHLGLQAPSPRDSDADSDAGKKESDGEEGRPPQPRREGPIGGESDSEEMFEKTSGFGSGKPSEASEPCVAELLRAREDSECLGSIRHEAERLERTMERLISDTEGFAADAGLQGLQGEGDQGEGGKNEPQLLGTINARMKAFRKELQAFLEQVNHIGDGLRERLEGLSPLPHLTESSSFLSTVTSVSRDSPIGTLGKELVPDLQSKLRDQMEWQLGPERGDEPEHLRLRAARELHRRADGDVGSCRLGGQSCFSPELRGPPVLPEQSVWIEELQGQLAQATRLHQEETEKFTNKIHKMEEEHLYALRWKELEMHSLALQNTLHERTWSDERNLMQQELRSLKQNIFLFYVKLRWLLKHWRQGKQMEEEGEDFTETEHPETLPRLGELGVQGDPKGDGPDREDDSPGCGLALGEHSPRSAVQMGERGPRLQPADGGQLHRQVVENQQLFGALKSLLEDLRSELREDEHARLRLQQQYASDKAAWDVEWAMLKCRLEQLEEKTEKNLGESGPSADSKGAFKKEREIHQKLLADSHGLVMDLRWQIHHSEKNWHREKVELLDRLDRDRQEWEQHKKELLWRIEQLQKENSPRRGGSFLCDQKEGNIRPFAHPGSPRVPRPLGLWPCSDTDSVPFDDRPLSKLKESDRCSARENLYLDTLSLDDEPEEPPARGPQREFGNCLPEEEENPKGNLQRAVSVSSMSEFQRLMDVSPFLPEKGLPAASGKEDVTPPLSPDDLKYIEEFNSKSWDERPLDPWADRTEAGRVGSEAGAEPIPDSSWYLTTSITMTTDTMTSPEHCQKQPLRSHVRAEPSGVRVLHSPPAVRRMDSIVVAGGEVQGRPEPECPFSTSRARGGTGDTKGVSSEHMLGRWPGVSSRHPRDYVEGGLRPLDSTLCTPLGFASPLHSLEMSKNMSDDMKEVAFSVRNAICSGPTEPLVRDMACQTNGSRTTGTQTIQTISVGLQTEALRGSAVTSSPHKCLTPKAGGGATPASSPSRGLRSRQVAPAIEKVQAKFERTCCSPKYGSPKLQRKPLPKADQPNNRTSPGLAQKGCSESAWARSTTTRESPVHTAINDGLSSLFNIIDHSPVVQDPFQKGMRAGSRSCSAEPRPELGLGQETSPTSRGQSPSPIGVGSETCREEGGEGTPVRQDLSAPPGYTLTENVARILNKKLLEHALKEERRQAPHGHPSLNSDSHMGEAVKAEPGSIEELPCSALAPTLEPCFSRPERPAHRRPPSRWAPHPPRPHWLSRPENPIPWRSSAMRSRPGRSSTREDASLHGLSWNNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L6D9</accession>
    <name>A0A8C4L6D9_EQUAS</name>
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        <recommendedName>
            <fullName evidence="6">Keratin, type I cytoskeletal 20</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Cytokeratin-20</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Keratin-20</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">KRT20</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterotetramer of two type I and two type II keratins. Associates with KRT8.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L6D9"/>
    <dbReference type="Ensembl" id="ENSEAST00005005407.2">
        <property type="protein sequence ID" value="ENSEASP00005004941.2"/>
        <property type="gene ID" value="ENSEASG00005003710.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161855"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000002">
        <property type="entry name" value="Type I keratin 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF167">
        <property type="entry name" value="KERATIN, TYPE I CYTOSKELETAL 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 9" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="12">
        <location>
            <begin position="76"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="80"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="186"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005004941.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="430" mass="49097" checksum="41DDF90E08C07372" modified="2023-09-13" version="2">MDFTGRSLHRSVSSSSRGPALSVSSSMYRLGGTQHLRVAPSVYGGAGGHGTRISTSHRVVSYGGDLSGGDLLVGNEKMAMQNLNDRLASYLEKVRSLEQSNLNLEVQIRQWYETNAPSASRDYSAYYKQIEDLRNQIKDAQLKNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLHRVLDDLTLRKTDLEIQIEELKKDLILLQKEHQEEVNSLRRQLGNTVSVEVDAAPGLNLSAIMDEMRQKYEAMAQENLRKAKEQFELQIETLQQEVIGTSKDLKETEGRIKELRRTYQNLEIDLQSYLSKKDSLERTLAETKARYGDKLATIQALLSTLEFQLMQIRTDAERQNNEYSILLDIKTRLEQEIATYRRLLEGEDVIIADYRSSHQEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L6L9</accession>
    <name>A0A8C4L6L9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Neogenin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">NEO1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Multi-functional cell surface receptor regulating cell adhesion in many diverse developmental processes, including neural tube and mammary gland formation, myogenesis and angiogenesis. Receptor for members of the BMP, netrin, and repulsive guidance molecule (RGM) families. Netrin-Neogenin interactions result in a chemoattractive axon guidance response and cell-cell adhesion, the interaction between NEO1/Neogenin and RGMa and RGMb induces a chemorepulsive response.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. DCC family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005007802.2">
        <property type="protein sequence ID" value="ENSEASP00005007147.2"/>
        <property type="gene ID" value="ENSEASG00005005240.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156684"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097708">
        <property type="term" value="C:intracellular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098797">
        <property type="term" value="C:plasma membrane protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070700">
        <property type="term" value="F:BMP receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039706">
        <property type="term" value="F:co-receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060586">
        <property type="term" value="P:multicellular organismal-level iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007520">
        <property type="term" value="P:myoblast fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048681">
        <property type="term" value="P:negative regulation of axon regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050709">
        <property type="term" value="P:negative regulation of protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030513">
        <property type="term" value="P:positive regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099550">
        <property type="term" value="P:trans-synaptic signaling, modulating synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05722">
        <property type="entry name" value="IgI_1_Neogenin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05723">
        <property type="entry name" value="IgI_4_Neogenin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000004">
        <property type="entry name" value="DCC isoform 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000316">
        <property type="entry name" value="Neogenin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000777">
        <property type="entry name" value="Neogenin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000101">
        <property type="entry name" value="Neogenin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000106">
        <property type="entry name" value="Neogenin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000133">
        <property type="entry name" value="Neogenin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000189">
        <property type="entry name" value="Neogenin isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000216">
        <property type="entry name" value="neogenin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000187">
        <property type="entry name" value="neogenin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010560">
        <property type="entry name" value="Neogenin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF14">
        <property type="entry name" value="NEOGENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06583">
        <property type="entry name" value="Neogenin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01832">
        <property type="entry name" value="VEGFRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <keyword evidence="4 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040296916" description="Neogenin" evidence="16">
        <location>
            <begin position="34"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="1097"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="52"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="152"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="243"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="341"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="461"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="561"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="656"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="761"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="860"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="961"/>
            <end position="1058"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1053"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1131"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1167"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1228"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1283"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1080"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1184"/>
            <end position="1199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1283"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1323"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="1359"/>
            <end position="1368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00055647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007147.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1454" mass="159151" checksum="3F85D7AC9DCF0FBF" modified="2023-09-13" version="2">MAAERGARRLLGTSSFWLCCLLLLGRRAPGAAAARSGSPPQTKGSGVRTFTPFYFLVEPVDTLSSRGSSVILNCSAYSEPSPKIEWKKDGTFLNLVSDDRRQLLPDGSLFITNVVHAKHNKPDEGYYQCVATVENLGTIVSRTAKLTVASLPRFASQPEPSSVYAGNSAVLNCEVNADLVPFVRWEQNRQPLLLDDRVIKLPSGTLVISNVTERDGGLYRCVVESGGPPKYSDEAELKVLPDPEVTSNLVFLKQPSSLVRVIGQSAVLPCVALGLPTPTIRWMKNEEALDTESSERLVLLAGGSLEISDVTEDDAGTYFCIADNGNETIEAQAELTVQAQPEFLKQPTNIYAHESMDIIFECEVTGKPAPTVKWVKNGDMVIPSDYFKIVKEHNLQVLGLVKSDEGFYQCIAENDVGNAQAGAQLIILEHDVAIPTLPPTSLTSATTDHLAPATTGPLPSAPRDVVASLVSTRFIKLTWRTPASDPHGDNLTYSVFYTKEGIARERVENTSHPGEMQVTIQNLMPATVYIFRVMAQNKHGSGESSAPLRVETQPEVQLPGPAPNIRAYATSPTSITVTWETPLSGNGEIQNYKLYYMEKGTDKEQDVDVSSHSHTINGLKKYTEYSFRVVAYNKHGPGVSTQDVAVRTLSDVPSAAPQNLSLEVRNSKSIMIHWQPPPPATQNGQITGYKIRYRKASRKSDVTETLVTGTQLSQLIEGLDRGTEYNFRVAALTINGTGPATDWLSAETFESDLDETRVPDVPSSLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPSSHYVITLKAFNNVGEGIPLYESAVTRPHTVPDPTPMMPPVGVQASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGLKANTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPRTIIVNWQPPSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSKGMGPMSEAVQFRTPKASGSSGKGSRLPDLGSDYKPPMSGSNSPHGSPTSPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSKDVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSYRGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQPVISAHPIHSLDNPHHHFHSSSLASPARSHLYHPGSPWPIGTSMSLSDRANSTESIRNTPSTDTMPASSSQTCCTDHQDPEGATSSSYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPPGPPTYDPTLPSTPLLSQQALNHHLHSVKTASIGTLGRSRPPMPVVVPSAPDVQETTRMLEDSESSYEPDELTKEMAHLEGLMKDLNAITTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L6X0</accession>
    <name>A0A8C4L6X0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Carbonic anhydrase-related protein 11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">CA-RP XI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CA11</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Does not have a catalytic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alpha-carbonic anhydrase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L6X0"/>
    <dbReference type="Ensembl" id="ENSEAST00005006905.2">
        <property type="protein sequence ID" value="ENSEASP00005006314.2"/>
        <property type="gene ID" value="ENSEASG00005004677.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162098"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004089">
        <property type="term" value="F:carbonate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03121">
        <property type="entry name" value="alpha_CARP_X_XI_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.200.10:FF:000002">
        <property type="entry name" value="Carbonic anhydrase-related protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.200.10">
        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041878">
        <property type="entry name" value="Alpha_CARP_X/XI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036398">
        <property type="entry name" value="CA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF93">
        <property type="entry name" value="CARBONIC ANHYDRASE-RELATED PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="10">
        <location>
            <begin position="26"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="292"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="312"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006314.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="321" mass="35367" checksum="BB42957B682AFCCE" modified="2023-09-13" version="2">SALPAPPPASLSPSPAYIGPAPDPEDWWSYKDNLQGNFVPGPPFWGLVNAAWSLCAVGKRQSPVDVELKRVLYDPFLPPLRLSTGGEKLRGTLYNTGRHVSFLPAARPVVNVSGGPLLYSHRLSELRLLFGARDGAGSEHQINHQGFSAEVQLIHFNQELYGNLSAASRGPNGLAILSLFVNVAGSSNPFLSRLLNRDTITRISYKNDAYFLQDLSLELLFPESFGFITYQGSLSTPPCSETVTWILIDRALNITSLQMHSLRLLSQNPPSQIFQSLSGNARPLQPLAHRALRGNRDPRHPERRCRGPNYRLHVDGAPHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LBB7</accession>
    <name>A0A8C4LBB7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Metallothionein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MT3</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Metallothioneins have a high content of cysteine residues that bind various heavy metals.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the metallothionein superfamily. Type 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LBB7"/>
    <dbReference type="SMR" id="A0A8C4LBB7"/>
    <dbReference type="Ensembl" id="ENSEAST00005007256.2">
        <property type="protein sequence ID" value="ENSEASP00005006635.1"/>
        <property type="gene ID" value="ENSEASG00005004901.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182967"/>
    <dbReference type="OMA" id="CEDSCKC"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016234">
        <property type="term" value="C:inclusion body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046870">
        <property type="term" value="F:cadmium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030295">
        <property type="term" value="F:protein kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071276">
        <property type="term" value="P:cellular response to cadmium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071280">
        <property type="term" value="P:cellular response to copper ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071294">
        <property type="term" value="P:cellular response to zinc ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071585">
        <property type="term" value="P:detoxification of cadmium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010273">
        <property type="term" value="P:detoxification of copper ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006112">
        <property type="term" value="P:energy reserve metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033210">
        <property type="term" value="P:leptin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030517">
        <property type="term" value="P:negative regulation of axon extension"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000376">
        <property type="term" value="P:positive regulation of oxygen metabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030949">
        <property type="term" value="P:positive regulation of vascular endothelial growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032095">
        <property type="term" value="P:regulation of response to food"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006829">
        <property type="term" value="P:zinc ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.10.10:FF:000001">
        <property type="entry name" value="Metallothionein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.10.10">
        <property type="entry name" value="Metallothionein Isoform II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017854">
        <property type="entry name" value="Metalthion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023587">
        <property type="entry name" value="Metalthion_dom_sf_vert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000006">
        <property type="entry name" value="Metalthion_vert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018064">
        <property type="entry name" value="Metalthion_vert_metal_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23299">
        <property type="entry name" value="METALLOTHIONEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23299:SF18">
        <property type="entry name" value="METALLOTHIONEIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00131">
        <property type="entry name" value="Metallothio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00860">
        <property type="entry name" value="MTVERTEBRATE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57868">
        <property type="entry name" value="Metallothionein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00203">
        <property type="entry name" value="METALLOTHIONEIN_VRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 5" id="KW-0480">Metal-thiolate cluster</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="68" mass="6885" checksum="59801B9CD1E52CE6" modified="2022-01-19" version="1">MDPETCPCPTGGSCTCSGSCKCEGCKCTSCKKSCCSCCPAECEKCAKDCVCKGGEGAEAEAEKCSCCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LBI9</accession>
    <name>A0A8C4LBI9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha</fullName>
            <ecNumber evidence="10">2.7.1.137</ecNumber>
            <ecNumber evidence="8">2.7.1.153</ecNumber>
            <ecNumber evidence="9">2.7.1.154</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="30">Phosphoinositide 3-kinase-C2-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="38" type="primary">PIK3C2A</name>
    </gene>
    <organism evidence="38 39">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="38 39" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="38" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="38" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4-bisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18373"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57658"/>
            <dbReference type="ChEBI" id="CHEBI:58178"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.154"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:18374"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12709"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58088"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.137"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:12710"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate) + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.153"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:21293"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
            <location evidence="4">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus</location>
            <location evidence="6">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.137" evidence="10"/>
    <dbReference type="EC" id="2.7.1.153" evidence="8"/>
    <dbReference type="EC" id="2.7.1.154" evidence="9"/>
    <dbReference type="RefSeq" id="XP_014703349.1">
        <property type="nucleotide sequence ID" value="XM_014847863.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044608018.1">
        <property type="nucleotide sequence ID" value="XM_044752083.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070347337.1">
        <property type="nucleotide sequence ID" value="XM_070491236.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005010496.2">
        <property type="protein sequence ID" value="ENSEASP00005009666.1"/>
        <property type="gene ID" value="ENSEASG00005006116.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835478"/>
    <dbReference type="KEGG" id="eai:106835478"/>
    <dbReference type="CTD" id="5286"/>
    <dbReference type="GeneTree" id="ENSGT00940000157813"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005942">
        <property type="term" value="C:phosphatidylinositol 3-kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016303">
        <property type="term" value="F:1-phosphatidylinositol-3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046934">
        <property type="term" value="F:1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035005">
        <property type="term" value="F:1-phosphatidylinositol-4-phosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030276">
        <property type="term" value="F:clathrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090050">
        <property type="term" value="P:positive regulation of cell migration involved in sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd04012">
        <property type="entry name" value="C2A_PI3K_class_II"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="C2B_PI3K_class_II"/>
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    </dbReference>
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        <property type="entry name" value="PI3Ka_II"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd05176">
        <property type="entry name" value="PI3Kc_C2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07289">
        <property type="entry name" value="PX_PI3K_C2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.70:FF:000007">
        <property type="entry name" value="phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha"/>
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    <dbReference type="FunFam" id="2.60.40.150:FF:000116">
        <property type="entry name" value="Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta"/>
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        <property type="entry name" value="Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta"/>
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    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
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    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase, accessory domain (PIK)"/>
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        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
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        <property type="entry name" value="C2_dom"/>
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    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037705">
        <property type="entry name" value="PI3-kinase_C2-alpha_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015433">
        <property type="entry name" value="PI3/4_kinase"/>
    </dbReference>
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        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
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    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
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    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002420">
        <property type="entry name" value="PI3K-type_C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001263">
        <property type="entry name" value="PI3K_accessory_dom"/>
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    <dbReference type="InterPro" id="IPR042236">
        <property type="entry name" value="PI3K_accessory_sf"/>
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    <dbReference type="InterPro" id="IPR000341">
        <property type="entry name" value="PI3K_Ras-bd_dom"/>
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    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
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    <dbReference type="InterPro" id="IPR042133">
        <property type="entry name" value="PX_PI3K_C2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048:SF28">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL 4-PHOSPHATE 3-KINASE C2 DOMAIN-CONTAINING SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10048">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00792">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00794">
        <property type="entry name" value="PI3K_rbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00613">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00142">
        <property type="entry name" value="PI3K_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00144">
        <property type="entry name" value="PI3K_rbd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00145">
        <property type="entry name" value="PI3Ka"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51547">
        <property type="entry name" value="C2_PI3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00915">
        <property type="entry name" value="PI3_4_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51546">
        <property type="entry name" value="PI3K_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51545">
        <property type="entry name" value="PIK_HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="20" id="KW-0007">Acetylation</keyword>
    <keyword evidence="19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="25" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="15" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="12" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="21" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="18" id="KW-0418">Kinase</keyword>
    <keyword evidence="22" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="23" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="39" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PI3K-RBD" evidence="36">
        <location>
            <begin position="424"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="C2 PI3K-type" evidence="37">
        <location>
            <begin position="685"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="domain" description="PIK helical" evidence="35">
        <location>
            <begin position="864"/>
            <end position="1040"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="34">
        <location>
            <begin position="1108"/>
            <end position="1386"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="33">
        <location>
            <begin position="1425"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="32">
        <location>
            <begin position="1558"/>
            <end position="1681"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="617"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="31">
        <location>
            <begin position="631"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00006209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00012013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00012073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00029297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00069404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00079346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS51545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="36">
        <source>
            <dbReference type="PROSITE" id="PS51546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="37">
        <source>
            <dbReference type="PROSITE" id="PS51547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="39">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1689" mass="190859" checksum="4A8DD6426CAE9DF9" modified="2022-01-19" version="1">MAQISSNNGFKQCSSSHLELTRTKDVDKAEALQMEAEALAKLQKDRRVTDNQRGFELSSSTRQKAQVYNKQDYDLMVFPESDTQKRAVDIDVEKLTQAELEKLLLDDSFETRTAPVLPVTPVLSPSFSAQLYLRPTIQRGQWPPGLSGPSTYTLPSIYQSTYSSKQTSFQNGFNPRMPTFPSTEPIYLSLPGQSPYFSYPLTPATHFHPQASLPICRPLVSPDMAKLFDKIASTSEFLKNGKARTDLEITNSKATISNLQVSPKSEDISKFDWLDLDPLSKPKVDSVEVLDHEEEKNAPGLLAEDPWDAVLLEERSLASCHLERKVNGKSLSGATVTRSQSLNIRTTQLTKVQGQMSQKDSNGTSSLPTGSSLLQEIEVQNEEVAAFCQSITTLKINFPYTNHYTNPGYLLSPVTVQRNICGENASVKISIEIEGFQLPVTFTCDVSSTVEIVIMQALCWVHDDLNQVDVGSYVLKVCGQEEVLQNNHCLGSHEHIQNCRKWDTEIKLQLLTFSSMCQNLARTAEDDETPVDLNKYLHQIEKPYKEVMTRHPVEELLDSYHNQVELALQIENQHRAVDQVIKAVRKICSALDGVETLAITESVKKLKRAVNLPRSKSANMTSLSGREDTSKSSTRGSLNSENPVQVSMDQLTAAIYDLLRLHASSGGSPADCTQSNRNIKEAWTTTEQLQFTIFAAHGISSNWVSNYEKYYLICSLSYNGKDLFKPIQSKKVGTYKNFFYLIKWDELIIFPIQISQLPLECLLHLTLFGILNQSSGSSPDSNKQRKGPEALGKVSLPLFDFKRFLTCGTKLLYLWTSSHTNPVPGTVPKKGYVMERIVLQVDFPSPTFDIVYTTPQVDRSIIQQHNLETLENDIKGKLLDILHRDSSLGLSKEDKAFLWEKRYYCFKHPNCLPKVLASAPNWKRVNLAKTYSLLQEWPPLHPLTALELLDSKFADQEVRSLAVTWIEAISDDELTDLLPQFVQALKYEIYLNSSLVRFLLSRALGNIQIAHNLYWLLRDALHDAQFGARYEHVLGALLSVGGKGLREELLKQTKLVQLLGGVAEKVRQASGSARQVVLQRSMERVQSFFLKNKCRLPLNPSLVAKELNIKSCSFFSSNAVPLKVTMVNADPMGEEINVMFKVGEDLRQDMLALQMIKIMDKIWLKEGLDLRMVIFKCLSTGRDRGMVELVPASDTLRKIQVEYGVTGSFKDKPLAEWLRKYNPSEEEYEKASENFIYSCAGCCVATYVLGICDRHNDNIMLRSTGHMFHIDFGKFLGHAQMFGSFKRDRAPFVLTSDMAYVINGGEKPTIRFQLFVDLCCQAYNLIRKQTNLFLNLLSLMIPSGLPELTSIQDLKYVRDALQPQTTDAEATIFFTRLIESSLGSIATKFNFFIHNLAQLRFSGLPSNDEPILSFSPKTYSFRQDGRIKEVSVFTYHKKYNPDKHYIYVVRILREGQLEPSFVFRTFDEFQELHNKLSIIFPLWKLPGFPNRMVLGRTHIKDVAAKRKIELNSYLQSLMSASTDVAECDLVCTFFHPLLRDEKAEGIARSADAGPFSPTPGQIGGAVKLSVSYRNGTLFIMVMHIKDLVTEDGADPNPYVKTYLLPDTHKTSKRKTKISRKTRNPTFNEMLVYSGYSKETLRQRELQLSVLSAESLRENFFLGGITLPLKDFNLSKETVKWYQLTAATYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LCG8</accession>
    <name>A0A8C4LCG8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ankyrin repeat and death domain containing 1A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ANKDD1A</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
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                <person name="Yin"/>
                <person name="Q."/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006249.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="606" mass="68073" checksum="CC5CEA28C0F31795" modified="2023-09-13" version="2">MLHTATSCWPPFLGLAVVLIFMGSTIGCPARCECSAQNKSVSCHRRRLIAIPEGIPIETKILDLSKNRLKSVNPEEFISYPLLEEIDLSDNIIANVEPGAFNNLFNLRSLRLKGNRLKLVPLGVFTGLSNLTKLDISENKIVILLDYMFQDLHNLKSLEVGDNDLVYISHRAFSGLLSLEQLTLEKCNLTAVPTEALSHLRSLISLHLKHLNINNMPVYAFKRLFHLKHLEIDYWPLLDMMPANSLYGLNLTSLSITNTNLSTVPFLAFKHLVYLTHLNLSYNPISTIEAGMFSDLIRLQELHIVGAQLRTIEPHSFQGLHFLRVLNVSQNLLETLEENVFSSPRALEVLSINNNPLACDCRLLWILQRHPTLQFGGQQPMCAGPDTIRERSFKDFHSTALSFYFTCKKPKIRDKKLQHLLVDEGQTVQLECSADGDPQPVISWVTPRRRFITTKSNGRATVLGDGTLEIRFAQDQDSGMYVCIASNAAGNDTFTASLTVKGFASDRFLYANRTPMYMTDSNDTIFNGTNANTFSLDLKTILVSTAMGCFTFLGVVLFCFLLLFVWSRGKGKHKNSIDLEYVPRKNNGAVVEGEVAGPRRFNMKMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LDA3</accession>
    <name>A0A8C4LDA3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arpin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ARPIN</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Arpin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044617253.1">
        <property type="nucleotide sequence ID" value="XM_044761318.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LDA3"/>
    <dbReference type="Ensembl" id="ENSEAST00005009843.2">
        <property type="protein sequence ID" value="ENSEASP00005009037.1"/>
        <property type="gene ID" value="ENSEASG00005006475.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831986"/>
    <dbReference type="KEGG" id="eai:106831986"/>
    <dbReference type="CTD" id="348110"/>
    <dbReference type="GeneTree" id="ENSGT00530000064251"/>
    <dbReference type="OMA" id="QTVAFWI"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033058">
        <property type="term" value="P:directional locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051126">
        <property type="term" value="P:negative regulation of actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030336">
        <property type="term" value="P:negative regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000393">
        <property type="term" value="P:negative regulation of lamellipodium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018889">
        <property type="entry name" value="Arpin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31199">
        <property type="entry name" value="ARPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31199:SF1">
        <property type="entry name" value="ARPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10574">
        <property type="entry name" value="UPF0552"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="203"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="211"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="226" mass="25016" checksum="1957EB11F373E304" modified="2022-01-19" version="1">MSRIYRDDALRNKPVRSARLPGAWAPAAHQRGNGVLLEGDLVDVSRHSISDAHGRKERYYVLYIRPSCIHRRKFDPKGNEIEPNFSATRKVNTGFLMSSYKEEAKGDTDRLTPEALKGLVNKPELLALTESLTPEQTVAFWMLESEMEAMELELGAGVRLKTRGDGPFLDSLAKLEAGTVTKCNFAGDGKTGASWTDNIMARKSSEGAAVESREQGDGAEDKEWDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LL02</accession>
    <name>A0A8C4LL02_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">IF rod domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106835268</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LL02"/>
    <dbReference type="Ensembl" id="ENSEAST00005013829.2">
        <property type="protein sequence ID" value="ENSEASP00005012723.2"/>
        <property type="gene ID" value="ENSEASG00005008871.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154026"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030280">
        <property type="term" value="F:structural constituent of skin epidermis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031424">
        <property type="term" value="P:keratinization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000004">
        <property type="entry name" value="Keratin, type II cytoskeletal 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032444">
        <property type="entry name" value="Keratin_2_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003054">
        <property type="entry name" value="Keratin_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616">
        <property type="entry name" value="GATA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616:SF51">
        <property type="entry name" value="IF ROD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16208">
        <property type="entry name" value="Keratin_2_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01276">
        <property type="entry name" value="TYPE2KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="7">
        <location>
            <begin position="126"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="335"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005012723.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="480" mass="53269" checksum="96DB9EF2F31C8AA5" modified="2023-09-13" version="2">MTCGSARFSSCSGVRTSSSCVATQGYSSGRTFSCSSACGPRPGRCCITAAPYRGISCYRGLASGFGSRSVCGGVRAGSSGRSFGYRSGGLCGLSAPSITTVSVNESLLVPLNLEMDPNAQGVKQKEKEQIKSLNNKFAAFIDKVRFLEQQNKLLQTKWQFYQNRKCCDSNLEPLFSGYIETLRREAERVEADSGRLASELNHVQEVLEGYKKKYEEEVTLRTTAENEFVRIKQEVNGVYVRKGDLEANAHSLVEEISFLKSLYEEEIRVLQAHISDTSVIVKMDNSRDLNMDSILAEIKAHYDDIASRSRAEAESWYRGKYEEIKATVVRHGETLRRTKEEINELNRVIQRLTAEINNAKSQNSKLEAAVSQAEQQGEVALTDARRKLAGLEEALQKAKQDMARLLREYQEVLSSKLGLDIEIATYRRLLEGEEQRWVQPARLGVSNSQGGVVCGDLSTTRTCTIKSRGVGICGSSYKKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LLT6</accession>
    <name>A0A8C4LLT6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="19">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">FRK</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 19">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts (via the SH3-domain) with PTEN. Interacts with RB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="19"/>
    <dbReference type="RefSeq" id="XP_014721638.1">
        <property type="nucleotide sequence ID" value="XM_014866152.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LLT6"/>
    <dbReference type="SMR" id="A0A8C4LLT6"/>
    <dbReference type="Ensembl" id="ENSEAST00005012979.2">
        <property type="protein sequence ID" value="ENSEASP00005011944.1"/>
        <property type="gene ID" value="ENSEASG00005008367.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106847040"/>
    <dbReference type="KEGG" id="eai:106847040"/>
    <dbReference type="CTD" id="2444"/>
    <dbReference type="GeneTree" id="ENSGT00940000156987"/>
    <dbReference type="OMA" id="SSHEGWW"/>
    <dbReference type="OrthoDB" id="4062651at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002753">
        <property type="term" value="P:cytoplasmic pattern recognition receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045824">
        <property type="term" value="P:negative regulation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd05068">
        <property type="entry name" value="PTKc_Frk_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10369">
        <property type="entry name" value="SH2_Src_Frk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11845">
        <property type="entry name" value="SH3_Src_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000630">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000217">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000037">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000044">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035805">
        <property type="entry name" value="SH2_Frk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 18 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 18 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 16" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="3 17" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 19" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="SH3" evidence="22">
        <location>
            <begin position="51"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="21">
        <location>
            <begin position="125"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="243"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="271"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="515" mass="59047" checksum="A1C3A5B988B16C17" modified="2022-01-19" version="1">MGNVCLRLRAYLEPCLPCLFQEADKPVVIENPGARSLPDAPQPPSQEPERSHGHYFVALFDYQARTAEDLSFRAGDKLQVLDASHEGWWFARHLEKRGDGSGQQLQGYIPSNYVAEDRSLQAEPWFFGAIKRADAEKQLLYSENQTGAFLIRESESQKGEFSLSVLHGRVVKHYRIRRLDEGGFFLTRRQTFSTLNEFVSHYMKTSDGLCVKLGKPCLKVEVPAPFDLSYKTVDQWEIDRNSVQLLKRLGSGQFGEVWEGLWNNTTPVAVKTLKPGSMDPNDFLREAQIMKNLRHPKLIQLYAVCTLEDPIYIITELMRHGSLQEYLQNDAGSKIHLPQQVDMAAQVASGMAYLESQNYIHRDLAARNVLVGEHNIYKVADFGLARVFKVDNEDIYESKHEIKLPVKWTAPEAIRSNKFSIKSDVWSFGILLYEIITYGKMPYSGMTGAQVIQMLGQNYRLPQPSNCPLQFYNIMLECWNAEPKERPTFETLHWKLEDYFETDSSSYSDTENFVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LMA3</accession>
    <name>A0A8C4LMA3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Urocortin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">UCN</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sauvagine/corticotropin-releasing factor/urotensin I family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014705853.1">
        <property type="nucleotide sequence ID" value="XM_014850367.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LMA3"/>
    <dbReference type="Ensembl" id="ENSEAST00005014475.2">
        <property type="protein sequence ID" value="ENSEASP00005013329.1"/>
        <property type="gene ID" value="ENSEASG00005009290.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837043"/>
    <dbReference type="KEGG" id="eai:106837043"/>
    <dbReference type="CTD" id="7349"/>
    <dbReference type="GeneTree" id="ENSGT00940000154473"/>
    <dbReference type="OMA" id="QNRIVFD"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000252">
        <property type="term" value="P:negative regulation of feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051461">
        <property type="term" value="P:positive regulation of corticotropin secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010996">
        <property type="term" value="P:response to auditory stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001964">
        <property type="term" value="P:startle response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018446">
        <property type="entry name" value="Corticotropin-releasing_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000187">
        <property type="entry name" value="CRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003620">
        <property type="entry name" value="Urocortin_CRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15035">
        <property type="entry name" value="CORTICOLIBERIN/UROCORTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15035:SF11">
        <property type="entry name" value="UROCORTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00473">
        <property type="entry name" value="CRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01612">
        <property type="entry name" value="CRFFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00039">
        <property type="entry name" value="CRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00511">
        <property type="entry name" value="CRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0027">Amidation</keyword>
    <keyword evidence="4" id="KW-0372">Hormone</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034828596" evidence="6">
        <location>
            <begin position="25"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Corticotropin-releasing factor" evidence="7">
        <location>
            <begin position="85"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005013329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="126" mass="14075" checksum="F091A087DCEEC70B" modified="2022-01-19" version="1">MRQAGRAALLAALLLLAQLRSGSSQWSPEVTEAGVQDPSLRWIPGVRNQSSGARALLLLLAERFPRRAGPRRWVSGTAGERPRRDDPPLSIDLTFHLLRTLLELARTQSQRERAEQNRIIFDSVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LNH4</accession>
    <name>A0A8C4LNH4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Nipsnap homolog 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NIPSNAP2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NipSnap family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044603689.1">
        <property type="nucleotide sequence ID" value="XM_044747754.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LNH4"/>
    <dbReference type="Ensembl" id="ENSEAST00005013815.2">
        <property type="protein sequence ID" value="ENSEASP00005012712.2"/>
        <property type="gene ID" value="ENSEASG00005008881.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831836"/>
    <dbReference type="KEGG" id="eai:106831836"/>
    <dbReference type="CTD" id="2631"/>
    <dbReference type="GeneTree" id="ENSGT00950000183018"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000003">
        <property type="entry name" value="Protein NipSnap homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000007">
        <property type="entry name" value="protein NipSnap homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012577">
        <property type="entry name" value="NIPSNAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051557">
        <property type="entry name" value="NipSnap_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21017">
        <property type="entry name" value="NIPSNAP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21017:SF14">
        <property type="entry name" value="PROTEIN NIPSNAP HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07978">
        <property type="entry name" value="NIPSNAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0072">Autophagy</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="NIPSNAP" evidence="6">
        <location>
            <begin position="187"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005012712.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="286" mass="33536" checksum="81639FA2D505AF46" modified="2023-09-13" version="2">MAARVLYAHGAAAAGGLLRRAAPCNLLPGLRRLTSSDNRPREDSWLKSLFVRKVDPRKDAHSNLLAKKETSSLYKLQFHNVKPECLDAYNKICQEVLPKVHEDKHYPCTLVGTWNTWYGEQDQAVHLWRYEGGYPALTEVMNKLREDQEFVEFRKARSNMLLSRKNQLLLEFSFWNEPVPRSGPNIYELRSYQLRPGTMIEWGNYWARAIRFRQDSNEAVGGFFSQIGQLYMVHHLWAYKDLQIREDIRNAAWHKHGWEELVYYTVPLIQEMESRIMIPLKTSPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LPM0</accession>
    <name>A0A8C4LPM0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome b-c1 complex subunit 1, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Complex III subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Core protein I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Ubiquinol-cytochrome-c reductase complex core protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">UQCRC1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 11 subunits. The complex is composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein UQCRFS1, 2 core protein subunits UQCRC1/QCR1 and UQCRC2/QCR2, and 6 low-molecular weight protein subunits UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and subunit 9, the cleavage product of Rieske protein UQCRFS1. The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and cytochrome c oxidase (complex IV, CIV), resulting in different assemblies (supercomplex SCI(1)III(2)IV(1) and megacomplex MCI(2)III(2)IV(2)). Interacts with UQCC6. Interacts with STMP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peptidase M16 family. UQCRC1/QCR1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070348421.1">
        <property type="nucleotide sequence ID" value="XM_070492320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LPM0"/>
    <dbReference type="Ensembl" id="ENSEAST00005015020.2">
        <property type="protein sequence ID" value="ENSEASP00005013835.2"/>
        <property type="gene ID" value="ENSEASG00005009425.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106830662"/>
    <dbReference type="CTD" id="7384"/>
    <dbReference type="GeneTree" id="ENSGT00940000158931"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000016">
        <property type="entry name" value="Cytochrome b-c1 complex subunit 1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000001">
        <property type="entry name" value="Mitochondrial-processing peptidase subunit beta, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF116">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="18">
        <location>
            <begin position="118"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="19">
        <location>
            <begin position="270"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00061208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00076278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00081098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00082886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005013835.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="540" mass="58909" checksum="3581CAF0847408C8" modified="2023-09-13" version="2">MSVGKGGRADRTSKQPRVNILDPRQGAGESQVCGTAPAPRAPPRLLYACVALPSADWSLKMAASAVCRAACCGTRVLLHTCRSPALLRSPALRGTATFAQALQSVPETQVSLLDNGLRVASEQSSQPTCTVGVWIDVGSRYETEKNNGAGYFVEHLAFKGTKNRPGNALEKEVESIGAHLNAYSTREHTAYYIKALSKDLPKAVELLADIVQNCSLEDSQIEKERDVILQELQENDASMRDVVFDYLHATAFQGTPLAQAVEGPSENVRKLSRADLTEYLSRHYKAPRMVLAAAGGVEHRQLLDLAQKHFSSISGTYTEDAVPTLAPCRFTGSEIRHRDDALPLAHVAIAVEGPGWANPDNVALQVANAIIGHYDCTYGGGTHLSSPLASVAVANKLCQSFQTFSICYAETGLLGAHFVCDRMSIDDMMFFLQGQWMRLCTSATESEVVRGKNILRNALVSHLDGTTPVCEDIGRSLLTYGRRIPLAEWESRIAEVDASVVREVCSKYLYDQCPAVAGFGPIEQLPDYNRIRSGMFWLRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LSA0</accession>
    <name>A0A8C4LSA0_EQUAS</name>
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        <submittedName>
            <fullName evidence="16">Leucine rich repeat containing 52</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LRRC52</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014696644.1">
        <property type="nucleotide sequence ID" value="XM_014841158.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LSA0"/>
    <dbReference type="SMR" id="A0A8C4LSA0"/>
    <dbReference type="Ensembl" id="ENSEAST00005016297.2">
        <property type="protein sequence ID" value="ENSEASP00005014978.2"/>
        <property type="gene ID" value="ENSEASG00005010476.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831130"/>
    <dbReference type="KEGG" id="eai:106831130"/>
    <dbReference type="CTD" id="440699"/>
    <dbReference type="GeneTree" id="ENSGT00940000156906"/>
    <dbReference type="OrthoDB" id="4691307at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099104">
        <property type="term" value="F:potassium channel activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005249">
        <property type="term" value="F:voltage-gated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051649">
        <property type="term" value="P:establishment of localization in cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000015">
        <property type="entry name" value="Leucine rich repeat containing 38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051432">
        <property type="entry name" value="KCNMA1_auxiliary"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000372">
        <property type="entry name" value="LRRNT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46473">
        <property type="entry name" value="GH08155P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46473:SF6">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00013">
        <property type="entry name" value="LRRNT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
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    <keyword evidence="7" id="KW-0677">Repeat</keyword>
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    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040440927" evidence="14">
        <location>
            <begin position="24"/>
            <end position="315"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="245"/>
            <end position="273"/>
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    <feature type="domain" description="LRRNT" evidence="15">
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            <begin position="25"/>
            <end position="57"/>
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            <dbReference type="ARBA" id="ARBA00004162"/>
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    <sequence length="315" mass="35127" checksum="2F1E6167926119B6" modified="2023-09-13" version="2">MSLASSPGPGWLLFSFGMGLVSGSKCPNNCECQAQEVICTGLELTEYPPDIPLDTRRLFLDDNCITFLPAMTLGLLSDLVYLDCQNNLIQEVMDYTFIGVFKLIYLNLSYNNLTLISPYSFSVLSNLVQLNISNNPHLLSLNKYTFANTSSLRYLDLRNTGLQTLDHAAFHNILTLHTVYLSGNPWKCNCSFLDFTIFLIVSDLDHPDDANATCVEPTELAGWPITQVGNPLRYMCITHLDRQDYIFLLLIGFCIFSAGTVAAWLTGVCAVLYQNALRKSSEDEAEDEHGQRVEVSRTIFPHSADSGHDGFPQLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LSW1</accession>
    <name>A0A8C4LSW1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Sodium channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">SCN8A</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.</text>
    </comment>
    <comment type="function">
        <text evidence="21">Pore-forming subunit of a voltage-gated sodium channel complex assuming opened or closed conformations in response to the voltage difference across membranes and through which sodium ions selectively pass along their electrochemical gradient. Contributes to neuronal excitability by regulating action potential threshold and propagation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 23">Cell membrane</location>
            <topology evidence="2 23">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Axon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.6/SCN8A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005013536.2">
        <property type="protein sequence ID" value="ENSEASP00005012468.2"/>
        <property type="gene ID" value="ENSEASG00005008696.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156263"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031402">
        <property type="term" value="F:sodium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005248">
        <property type="term" value="F:voltage-gated sodium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0086010">
        <property type="term" value="P:membrane depolarization during action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13433">
        <property type="entry name" value="Na_channel_gate"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Sodium channel protein"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Sodium channel protein"/>
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        <property type="entry name" value="Voltage-dependent sodium channel"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="4"/>
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    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.20.5.1190">
        <property type="entry name" value="iswi atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
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        <property type="entry name" value="Ion_trans_dom"/>
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    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
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    <dbReference type="InterPro" id="IPR058542">
        <property type="entry name" value="IQ_SCN5A_C"/>
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    <dbReference type="InterPro" id="IPR008054">
        <property type="entry name" value="Na_channel_a8su"/>
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    <dbReference type="InterPro" id="IPR001696">
        <property type="entry name" value="Na_channel_asu"/>
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    <dbReference type="InterPro" id="IPR044564">
        <property type="entry name" value="Na_chnl_inactivation_gate"/>
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    <dbReference type="InterPro" id="IPR010526">
        <property type="entry name" value="Na_trans_assoc_dom"/>
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    <dbReference type="InterPro" id="IPR024583">
        <property type="entry name" value="Na_trans_cytopl"/>
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    <dbReference type="InterPro" id="IPR043203">
        <property type="entry name" value="VGCC_Ca_Na"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037:SF23">
        <property type="entry name" value="SODIUM CHANNEL PROTEIN TYPE 8 SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10037">
        <property type="entry name" value="VOLTAGE-GATED CATION CHANNEL CALCIUM AND SODIUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24609">
        <property type="entry name" value="IQ_SCN5A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06512">
        <property type="entry name" value="Na_trans_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11933">
        <property type="entry name" value="Na_trans_cytopl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00170">
        <property type="entry name" value="NACHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01667">
        <property type="entry name" value="NACHANNEL8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="19 23" id="KW-0407">Ion channel</keyword>
    <keyword evidence="13 23" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 23" id="KW-0915">Sodium</keyword>
    <keyword evidence="4 23" id="KW-0894">Sodium channel</keyword>
    <keyword evidence="17 23" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 23" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10 23" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="128"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="199"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="387"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="764"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="794"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="874"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="961"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1209"/>
            <end position="1227"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1248"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1279"/>
            <end position="1302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1323"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1448"/>
            <end position="1474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1533"/>
            <end position="1551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1563"/>
            <end position="1581"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1593"/>
            <end position="1613"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1650"/>
            <end position="1678"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1753"/>
            <end position="1776"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="25">
        <location>
            <begin position="131"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="domain" description="Voltage-gated Na+ ion channel cytoplasmic" evidence="27">
        <location>
            <begin position="545"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="25">
        <location>
            <begin position="763"/>
            <end position="993"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium ion transport-associated" evidence="26">
        <location>
            <begin position="1001"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="25">
        <location>
            <begin position="1207"/>
            <end position="1483"/>
        </location>
    </feature>
    <feature type="domain" description="Ion transport" evidence="25">
        <location>
            <begin position="1532"/>
            <end position="1786"/>
        </location>
    </feature>
    <feature type="domain" description="SCN5A-like C-terminal IQ motif" evidence="28">
        <location>
            <begin position="1898"/>
            <end position="1924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="28"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="446"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="567"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1117"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1932"/>
            <end position="1990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="28"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
        <location>
            <begin position="474"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="24">
        <location>
            <begin position="489"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="501"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="585"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="24">
        <location>
            <begin position="1948"/>
            <end position="1959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="24">
        <location>
            <begin position="1961"/>
            <end position="1990"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00058180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00061447"/>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU361132"/>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF00520"/>
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    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF06512"/>
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    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF11933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF24609"/>
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    </evidence>
    <evidence type="ECO:0000313" key="29">
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            <dbReference type="Ensembl" id="ENSEASP00005012468.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="1990" mass="226320" checksum="2FB14E5C548BF374" modified="2023-09-13" version="2">MAARLLAPPGPDSFKPFTPESLANIERRIAESKLKKPPKADGSHREDDEDSKPKPNSDLEAGKSLPFIYGDIPQGLVAVPLEDFDPYYLTQKTFVVLNRGKTLFRFSATPALYILSPFNLIRRIAIKILIHSVFSMIIMCTILTNCVFMTFSNPPEWSKNVEYTFTGIYTFESLVKIIARGFCIDGFTFLRDPWNWLDFSVIMMAYITEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVVWPINFNESYLENGTKGFDWEEYINNKTNFYTVPGMLEPLLCGNSSDAGQCPEGYQCMKAGRNPNYGYTSFDTFSWAFLALFRLMTQDYWENLYQLTLRAAGKTYMIFFVLVIFVGSFYLVNLILAVVAMAYEEQNQATLEEAEQKEAEFKAMLEQLKKQQEEAQAAAMATSAGTVSEDAIEEEGEEGGGSPRSSSELSKLSSKSAKERRNRRKKRKQKELSEGEEKGDPEKVFKSESEDGMRRKGFRLPDNRIGRKFSIMNSLLSIPGSPFLSRHNSKSSIFSFRGPGRFRDPGSENEFADDEHSTVEESEGRRDSLFIPIRARERRSSYSGYSGYSQGSRSSRIFPSLRRSVKRNSTVDCNGVVSLIGGPGSHIGGRLLPEVKIDKAATDHSATTEIEIKKKGPGSLLVSMDQLASYGRKDRINSILSVVTNTLVEELEESQRKCPPCWYKFANTFLIWECHPYWIKLKEIVNLIVMDPFVDLAITICIVLNTLFMAMEHHPMTPQFEHVLAVGNLVFTGIFTAEMFLKLIAMDPYYYFQEGWNIFDGFIVSLSLMELSLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINQDCELPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDGEMNNLQISVIRIKKGVAWIKLKVRAFMQAHFKQREADEVKPLDELYEKKANCIANHTGADIHRNGDFQKNGNGTTSGIGSSVEKYIIDEDHMSFINNPNLTVRVPIAVGESDFENLNTEDVSSESDPEGSKDKLDDTSSSEGSTIDIKPEVEEVPVEQPEEYLDPDACFTEGCVQRFKCCQVNIEEGLGKSWWILRKTCFLIVEHNWFETFIIFMILLSSGALAFEDIYIEQRKTIRTILEYADKVFTYIFILEMLLKWTAYGFVKFFTNAWCWLDFLIVAVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYHYCFNETSEIRFEIEIVNNKTDCEKLMEGNNTEIRWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRKPDEQPKYEDNIYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKIQGIIFDFVTQQAFDIVIMMLICLNMVTMMVETDTQSKQMENILYWINLVFVIFFTCECVLKMFALRHYYFTIGWNIFDFVVVILSIVGMFLADIIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYVKHEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLLPILNRPPDCSLDKEHPGSGFKGDCGNPSVGIFFFVSYIIISFLIVVNMYIAIILENFSVATEESADPLSEDDFETFYEIWEKFDPDATQFIEYCKLADFADALEHPLRVPKPNTIELIAMDLPMVSGDRIHCLDILFAFTKRVLGDSGELDILRQQMEERFVASNPSKVSYEPITTTLRRKQEEVSAVVLQRAYRGHLARRGFICKKTTSNKLENGGTHREKKESTPSTASLPSYDSVTKPEKEKQQRAEEGRRERAKRQKEVRESKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LV73</accession>
    <name>A0A8C4LV73_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">SCAN domain containing 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SCAND3</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014697980.1">
        <property type="nucleotide sequence ID" value="XM_014842494.3"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005019062.2">
        <property type="protein sequence ID" value="ENSEASP00005017557.1"/>
        <property type="gene ID" value="ENSEASG00005012137.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831983"/>
    <dbReference type="KEGG" id="eai:106831983"/>
    <dbReference type="CTD" id="114821"/>
    <dbReference type="GeneTree" id="ENSGT00940000160807"/>
    <dbReference type="OMA" id="VWSQLFK"/>
    <dbReference type="OrthoDB" id="10060419at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045787">
        <property type="term" value="P:positive regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050679">
        <property type="term" value="P:positive regulation of epithelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000001">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057560">
        <property type="entry name" value="Znf_SCAND3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913">
        <property type="entry name" value="EPM2A-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913:SF19">
        <property type="entry name" value="LOW QUALITY PROTEIN: ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23663">
        <property type="entry name" value="Znf_SCAND3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="4">
        <location>
            <begin position="52"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="5">
        <location>
            <begin position="363"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="570"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="709"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="246"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="541"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="709"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005017557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1326" mass="151835" checksum="D5B87DF2DC544B2F" modified="2022-01-19" version="1">MAVASRALPALAGQASEEQGEIIKVKVKEEDHVWDQESYLQKNLSDNRELSRQRFRQFCYHETPGPREALSQLRELCSQWLSPEIHTKEQILELLVLEQFLTILPEELQAWVREHNPKSGEEVVTVLEDLERELDEPRQQVPQGTYGQEVPMEEMTPLDTARESLGTQLQSMEDRMECESPEPRSLQDNGSFLWLSMMSQSMGDDNLSSLDTNEAEIEPENMREKFFRSLALLLENKSNNTKIFSKAKYCQLIREVKEAKAKAKKESVDYRRLARFDVILVQGNEKLIEAINGETDKIRYYLHSEDLFDILHDTHLSIGHGGRTRMEKELQAKYKNITKEVIMLYLTLCKLCQQKNSKLKKVLASKPIKEVHSRCQVDLIDMQLNPDGEYKFIMHYQDLRTKLSFLRSLKSKRPKEVAHALLDIFTIIGAPSVLQSDNGREFSSQIVSELSNIWPELKIVHGKPQPCQSQSSMNQINEDIQNRIVSWMQTNNSSHWAEFLWFIQMIQNQPYHRGMQQTPYEGTFNSEAKLGLSHSQLTEELVTSLNTENELEQANKELENTLRAQYEENIETGTDSSDSDENLSVTPKVVKKTPPESRLNFSSCVVCEKECTGAHSCISCDRNVHAFCGVPSQHETEGYHPRITCSLCYETTTMKRKHDGIQRSLTVQPSKMLKPSETPFSSEKVGDWMAKQASLDFFVKKRHTCSEYGSSGKRNGNNGNHPEEVKTKRVHTSFTRKYDPSYIEFGFVAVIDGEVLKPQCIICGDVLANEAMKPSKLKRHLYSKHKEISSQPKEFFERKSSELKSQPKQVFNVSHINISALRASYKVALPVAKSKTPYTIAETLVKDCIKEVCLEMLGESAAKKVAQVPLSNDTIARRIQELANDMEDQLIEQIKLAKYFSLQLDECRDIANMIILLVYVRFEHDDDIKEEFFFSASLPTNITSSELYEAIKNYIVNKCGLEFKWCVGVCSDGAASMTGKHSEVVTQIKALAPQCRITHCFIHRESLAMKKISAELNSVLTDIVKIVNYVKSNALNSRLFSLLCDNMETDHKQLLLHAEIRWLSRGKVLSRMFEIRNELLVFLQSKKPVWSQLFKDVNWTARLAYLSDIFSIFNDLNVSMQGKNATCFSMADKIEGQKQKLGAWKNRVSTDCYDMFHNLTTVINEVGHDLDIAHLRKVIKEHLTNLLDCFELYFPSKEDPRIGNSWIQNPFLSSKDNLNLTITLQDKLLKLATDEGLKMNFENTASLASFWINVKNEYPELAEIALKTLLLFPSTYLCETGFSTLSVIKTKHRNSLNIHYPLRVALSSIQPRLDKLTSKKQAHLSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LXS1</accession>
    <name>A0A8C4LXS1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Thyroid hormone receptor interactor 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">TRIP10</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the FNBP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044609119.1">
        <property type="nucleotide sequence ID" value="XM_044753184.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LXS1"/>
    <dbReference type="Ensembl" id="ENSEAST00005018305.2">
        <property type="protein sequence ID" value="ENSEASP00005016848.2"/>
        <property type="gene ID" value="ENSEASG00005011533.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106832731"/>
    <dbReference type="CTD" id="9322"/>
    <dbReference type="GeneTree" id="ENSGT00950000183047"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07653">
        <property type="entry name" value="F-BAR_CIP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11628">
        <property type="entry name" value="HR1_CIP4_FNBP1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11911">
        <property type="entry name" value="SH3_CIP4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000002">
        <property type="entry name" value="Formin-binding protein 1-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000017">
        <property type="entry name" value="Formin-binding protein 1-like isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057871">
        <property type="entry name" value="HR1_CIP4_FNBP1L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011072">
        <property type="entry name" value="HR1_rho-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057870">
        <property type="entry name" value="HR1_TOCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF17">
        <property type="entry name" value="CDC42-INTERACTING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25610">
        <property type="entry name" value="HR1_TOCA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51860">
        <property type="entry name" value="REM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 16 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="11" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="14" id="KW-0458">Lysosome</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 15" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="F-BAR" evidence="20">
        <location>
            <begin position="1"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="REM-1" evidence="21">
        <location>
            <begin position="337"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="19">
        <location>
            <begin position="485"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="281"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="424"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="124"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="341"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="289"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="314"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="441"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="474"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005016848.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="546" mass="62569" checksum="E4AB19F25129E73D" modified="2023-09-13" version="2">MDWGTELWDQFEVLERHTQWGLDLLDRYAKFVKERTEVEQAYAKQLRNLVKKYLPKRPVKDDPESKFSQHQSFVQILQEVNDFAGQRELVAENLSVRVCLELAKYSQEMKQERKMHFQEGRRAQQQLESGFKQLENSKRKFERDCREAEKAAQTAERLDQDINATKADVEKAKQQAHLRSHMAEESKNEYAAQLQRFNRDQAHFYFSQMPQIFDKLQDMDERRATHLGAGYGLLSDAELQVVPIIAKCLEGMKVAADAVDAKHDSQVLIELHKSGFARPGDVEFEDFSQPMNRAPSDSSLGTPSDGRPELRGPGRSRAKRWPFSKKNKTVVTEDFSHLPPEQQRKRLQQQLEGRNRELQKEIDQREALKKMKDVYEKTPQMGDPASLEPRITETLNNIERLKLEVQKYEAWLAEAESRVLSNRGDSLGRHARPPDPPASAPPDSGSNNNGSQDNKESSEEPPSEEGQDAPIYTEFDEDFEEEPASPIGHCVAIYHFEGSSEGTISMAEGEDLSLMEEDKGDGWTRVRRKQGGEGYVPTSYLRVTLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M7Q9</accession>
    <name>A0A8C4M7Q9_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 391</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZNF391</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014691038.1">
        <property type="nucleotide sequence ID" value="XM_014835552.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014691040.1">
        <property type="nucleotide sequence ID" value="XM_014835554.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044633011.1">
        <property type="nucleotide sequence ID" value="XM_044777076.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044633012.1">
        <property type="nucleotide sequence ID" value="XM_044777077.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070371554.1">
        <property type="nucleotide sequence ID" value="XM_070515453.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070371555.1">
        <property type="nucleotide sequence ID" value="XM_070515454.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070371556.1">
        <property type="nucleotide sequence ID" value="XM_070515455.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070371557.1">
        <property type="nucleotide sequence ID" value="XM_070515456.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070371558.1">
        <property type="nucleotide sequence ID" value="XM_070515457.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070371559.1">
        <property type="nucleotide sequence ID" value="XM_070515458.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070371561.1">
        <property type="nucleotide sequence ID" value="XM_070515460.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070371562.1">
        <property type="nucleotide sequence ID" value="XM_070515461.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070371563.1">
        <property type="nucleotide sequence ID" value="XM_070515462.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070371564.1">
        <property type="nucleotide sequence ID" value="XM_070515463.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070371565.1">
        <property type="nucleotide sequence ID" value="XM_070515464.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070371566.1">
        <property type="nucleotide sequence ID" value="XM_070515465.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070371567.1">
        <property type="nucleotide sequence ID" value="XM_070515466.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070371568.1">
        <property type="nucleotide sequence ID" value="XM_070515467.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M7Q9"/>
    <dbReference type="Ensembl" id="ENSEAST00005020708.2">
        <property type="protein sequence ID" value="ENSEASP00005019076.1"/>
        <property type="gene ID" value="ENSEASG00005030925.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005038707.1">
        <property type="protein sequence ID" value="ENSEASP00005052566.1"/>
        <property type="gene ID" value="ENSEASG00005030925.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005044789.1">
        <property type="protein sequence ID" value="ENSEASP00005035067.1"/>
        <property type="gene ID" value="ENSEASG00005030925.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005049791.1">
        <property type="protein sequence ID" value="ENSEASP00005061118.1"/>
        <property type="gene ID" value="ENSEASG00005030925.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005055785.1">
        <property type="protein sequence ID" value="ENSEASP00005043352.1"/>
        <property type="gene ID" value="ENSEASG00005030925.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005069209.1">
        <property type="protein sequence ID" value="ENSEASP00005049349.1"/>
        <property type="gene ID" value="ENSEASG00005030925.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106827622"/>
    <dbReference type="KEGG" id="eai:106827622"/>
    <dbReference type="CTD" id="346157"/>
    <dbReference type="GeneTree" id="ENSGT00940000153104"/>
    <dbReference type="OMA" id="QPYRCTQ"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000029">
        <property type="entry name" value="GLI family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000198">
        <property type="entry name" value="zinc finger protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001100">
        <property type="entry name" value="Zinc finger protein 184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000737">
        <property type="entry name" value="Zinc finger protein 565"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000953">
        <property type="entry name" value="Zinc finger protein 691"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050717">
        <property type="entry name" value="C2H2-ZF_Transcription_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196">
        <property type="entry name" value="ODD-SKIPPED - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14196:SF12">
        <property type="entry name" value="ZINC FINGER PROTEIN 208-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="137"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="277"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="305"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="333"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="63"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005019076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="358" mass="41008" checksum="8E5AD455C04BEB2A" modified="2022-01-19" version="1">MESLRGNTAQGPTNEEGCKSEDQLSRQTKCHVQKKSSFEKRTIRKVSMTLKEIFTRERGPESSEFSLSSKLHTQQKIPKGTRSLISRKNSKVNSNLIKHQKLFSQKKLCKCNECGKAFSYQSNLIVHSRIHDGEKPFECNECGKTFSRSTHLIEHQRTHTGEKPYECSECGKAFSRSTHLSLHQRIHTGEKPYECRECGKAFSRSTNLSQHQRTHTQEKPYKCNDCGKAFSDRSTIIQHQRIHTGENPYECSECGKAFSWISSLIEHQRTHTGENPYECSDCGKVFSRSSSLIEHQRIHTGEKPHECRECGKGFSRSSSLIIHQRTHTGEKPYKCNDCGKAFSQSSTLIRHQQLHSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M901</accession>
    <name>A0A8C4M901_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Toll-interacting protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TOLLIP</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the signaling pathway of IL-1 and Toll-like receptors. Inhibits cell activation by microbial products. Recruits IRAK1 to the IL-1 receptor complex. Inhibits IRAK1 phosphorylation and kinase activity. Connects the ubiquitin pathway to autophagy by functioning as a ubiquitin-ATG8 family adapter and thus mediating autophagic clearance of ubiquitin conjugates. The TOLLIP-dependent selective autophagy pathway plays an important role in clearance of cytotoxic polyQ proteins aggregates. In a complex with TOM1, recruits ubiquitin-conjugated proteins onto early endosomes. Binds to phosphatidylinositol 3-phosphate (PtdIns(3)P).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tollip family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044605820.1">
        <property type="nucleotide sequence ID" value="XM_044749885.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M901"/>
    <dbReference type="Ensembl" id="ENSEAST00005024206.2">
        <property type="protein sequence ID" value="ENSEASP00005022309.1"/>
        <property type="gene ID" value="ENSEASG00005015243.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106825297"/>
    <dbReference type="KEGG" id="eai:106825297"/>
    <dbReference type="CTD" id="54472"/>
    <dbReference type="GeneTree" id="ENSGT00390000013104"/>
    <dbReference type="OMA" id="IYIQIFD"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:protein-containing complex"/>
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        <property type="term" value="F:kinase binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:molecular adaptor activity"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:Toll-like receptor binding"/>
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        <property type="term" value="F:ubiquitin binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:innate immune response"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:interleukin-1-mediated signaling pathway"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0036010">
        <property type="term" value="P:protein localization to endosome"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04016">
        <property type="entry name" value="C2_Tollip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14363">
        <property type="entry name" value="CUE_TOLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000036">
        <property type="entry name" value="Toll-interacting protein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000055">
        <property type="entry name" value="Toll-interacting protein-like Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041799">
        <property type="entry name" value="TOLIP_CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037301">
        <property type="entry name" value="Tollip_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16461">
        <property type="entry name" value="TOLL-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR16461:SF5">
        <property type="entry name" value="TOLL-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="8" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="11">
        <location>
            <begin position="29"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="domain" description="CUE" evidence="12">
        <location>
            <begin position="229"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009278"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053704"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072084"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005022309.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="274" mass="30219" checksum="98E0D6DE3B6E343D" modified="2022-01-19" version="1">MATTVSTQRGPVYIGELPQDFLRILPTQQQQQIQLDAQAAQQLQFGGTVGTVGRLSITVVQAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIQCTVPPGVDSFYLEIFDERAFSMDDRIAWTHVTIPESLKQGKVLDEWYSLSGRQGDDKEGMINLVLSYTSLPAAMMMPPQPVVLMPTVYQQGVGYVPLTGVPAVCSPGMLPVALPPAAVSAQPRCNEEDLKAIQDMFPNMDREVIRSVLEAQRGNKDAAINSLLQMGEES</sequence>
</entry>
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    <accession>A0A8C4M9E6</accession>
    <name>A0A8C4M9E6_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Arkadia (RNF111) N-terminal like PKA signaling regulator 2N</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ARK2N</name>
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    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014828966.3"/>
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        <property type="nucleotide sequence ID" value="XM_044774664.2"/>
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        <property type="nucleotide sequence ID" value="XM_070513115.1"/>
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        <property type="protein sequence ID" value="ENSEASP00005023783.1"/>
        <property type="gene ID" value="ENSEASG00005016164.2"/>
    </dbReference>
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    <dbReference type="KEGG" id="eai:106823359"/>
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    <dbReference type="GeneTree" id="ENSGT00390000016167"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903901">
        <property type="term" value="P:negative regulation of viral life cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019046">
        <property type="term" value="P:release from viral latency"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029306">
        <property type="entry name" value="RNF111_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051073">
        <property type="entry name" value="ZNRF3_Arkadia_E3_ligases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16200">
        <property type="entry name" value="RING ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15303">
        <property type="entry name" value="RNF111_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="E3 ubiquitin-protein ligase Arkadia N-terminal" evidence="4">
        <location>
            <begin position="86"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="62"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="197"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="87"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="203"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005023783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="404" mass="43438" checksum="517717DAA647E505" modified="2022-01-19" version="1">MKMEEAVGKVEELIESEVPPKTSEKETAKEEDGSVELEAQVQKDAVVDSTVLSSMPCLLMELRRDSSESQLASTESDKPTTGRVYESDSSNHCMLSPSSSGHLADSDTLSSAEENEPSQAETAVEGDPSGVSGATVGRKSRRSRSESETSTMAAKKNRQSSDKQNGRVAKVKGHRSQKHKERIRLLRQKREAAARKKYNLLQDSSTSDSDLTCDSSTSSSDDDEEVSGSSKTITAEIPDGPPVVAHYDMSDTSSDPEVVNVDNLLAAAVVQEHSNSVGGQDTGATWRTSGLLEELNAEAGHLDPGFLASDKTSAGNAPLNEEINIASSDSEVEIVGVQEHARCVHPRGGVIQSVSSWKHGSGTQYVSSRQTQSWTAVTPQQTWASPAEVVDLTLDEDSRRKYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MGW2</accession>
    <name>A0A8C4MGW2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">THO complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">THOC2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="6">Component of the THO subcomplex, which is composed of THOC1, THOC2, THOC3, THOC5, THOC6 and THOC7. The THO subcomplex interacts with DDX39B to form the THO-DDX39B complex which multimerizes into a 28-subunit tetrameric assembly. Component of the transcription/export (TREX) complex at least composed of ALYREF/THOC4, DDX39B, SARNP/CIP29, CHTOP and the THO subcomplex; in the complex interacts with THOC1, THOC3, THOC5, THOC7 and DDX39B. TREX seems to have a dynamic structure involving ATP-dependent remodeling. Interacts with POLDIP3 and ZC3H11A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the THOC2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070359346.1">
        <property type="nucleotide sequence ID" value="XM_070503245.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005027732.2">
        <property type="protein sequence ID" value="ENSEASP00005025539.2"/>
        <property type="gene ID" value="ENSEASG00005017399.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106829908"/>
    <dbReference type="CTD" id="57187"/>
    <dbReference type="GeneTree" id="ENSGT00710000106792"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000445">
        <property type="term" value="C:THO complex part of transcription export complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040007">
        <property type="entry name" value="Tho2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021418">
        <property type="entry name" value="THO_THOC2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021726">
        <property type="entry name" value="THO_THOC2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032302">
        <property type="entry name" value="THOC2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21597:SF0">
        <property type="entry name" value="THO COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21597">
        <property type="entry name" value="THO2 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11262">
        <property type="entry name" value="Tho2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11732">
        <property type="entry name" value="Thoc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16134">
        <property type="entry name" value="THOC2_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="THO complex subunit 2 N-terminal" evidence="11">
        <location>
            <begin position="11"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunit 2 N-terminal" evidence="11">
        <location>
            <begin position="420"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunitTHOC2 N-terminal" evidence="10">
        <location>
            <begin position="568"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="domain" description="THO complex subunitTHOC2 C-terminal" evidence="9">
        <location>
            <begin position="873"/>
            <end position="1173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1184"/>
            <end position="1426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1466"/>
            <end position="1620"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="296"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="896"/>
            <end position="959"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1218"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1251"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1265"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1294"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1353"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1416"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1476"/>
            <end position="1531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1551"/>
            <end position="1609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1610"/>
            <end position="1620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
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            <dbReference type="Pfam" id="PF16134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MHI7</accession>
    <name>A0A8C4MHI7_EQUAS</name>
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        <submittedName>
            <fullName evidence="12">Neuronal PAS domain protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NPAS2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSEAST00005029114.2">
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        <property type="gene ID" value="ENSEASG00005018286.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 6"/>
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        <property type="term" value="C:CLOCK-BMAL transcription complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045739">
        <property type="term" value="P:positive regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051775">
        <property type="term" value="P:response to redox state"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="CDD" id="cd19737">
        <property type="entry name" value="bHLH-PAS_NPAS2_PASD4"/>
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        <property type="entry name" value="Circadian locomoter output cycles protein"/>
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        <property type="entry name" value="circadian locomoter output cycles protein kaput"/>
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        <property type="entry name" value="Circadian locomoter output cycles protein kaput"/>
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        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="9"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MKG0</accession>
    <name>A0A8C4MKG0_EQUAS</name>
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        <recommendedName>
            <fullName evidence="5">Cilia-and flagella-associated protein 96</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CFAP96</name>
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    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CFAP96 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MKG0"/>
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        <property type="protein sequence ID" value="ENSEASP00005026959.1"/>
        <property type="gene ID" value="ENSEASG00005018367.2"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00390000010980"/>
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    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 27"/>
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    <dbReference type="GO" id="GO:0097731">
        <property type="term" value="C:9+0 non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029358">
        <property type="entry name" value="CFAP96"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31144">
        <property type="entry name" value="UPF0602 PROTEIN C4ORF47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31144:SF1">
        <property type="entry name" value="UPF0602 PROTEIN C4ORF47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15239">
        <property type="entry name" value="CFAP96-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="310" mass="34691" checksum="79352875ECFE2261" modified="2022-01-19" version="1">MPAEGGKTDMERIGLFSEMEYVTVGDKYVSSFNRPFNEAASKNRQMLPGGSKEMSNLQAGYFDPHFVRIFEGEGYVNLNQVRRRRMMEEAKKNLSKAFLPSSGEKKPCGLGSYYGTIGGPVPFFSAQSKPREKYVVPGKNLYTSPGKKGTGYGYANITIGKQFSHSADFYDAPRLNYKKENEKHHDLLKGTPFKLNLYPREYFDSNPYLSEKPLPPLKKAEKREVLASPFKPCSPGKKAGGMKAGTFDPYPSHSADPYMVKSTNQISSKDVKIFHPPGGPKSRPIESIMTLNVKRALNMKNYKTASVQSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MMI7</accession>
    <name>A0A8C4MMI7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Mitogen-activated protein kinase kinase kinase 4</fullName>
            <ecNumber evidence="4">2.7.11.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">MAPK/ERK kinase kinase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">MAP3K4</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="4"/>
    <dbReference type="Ensembl" id="ENSEAST00005031450.2">
        <property type="protein sequence ID" value="ENSEASP00005028941.2"/>
        <property type="gene ID" value="ENSEASG00005019701.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106842047"/>
    <dbReference type="KEGG" id="eai:106842047"/>
    <dbReference type="CTD" id="4216"/>
    <dbReference type="GeneTree" id="ENSGT00880000138034"/>
    <dbReference type="OrthoDB" id="1043025at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060718">
        <property type="term" value="P:chorionic trophoblast cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019100">
        <property type="term" value="P:male germ-line sex determination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001890">
        <property type="term" value="P:placenta development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900745">
        <property type="term" value="P:positive regulation of p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032206">
        <property type="term" value="P:positive regulation of telomere maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010225">
        <property type="term" value="P:response to UV-C"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06626">
        <property type="entry name" value="STKc_MEKK4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000122">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045801">
        <property type="entry name" value="MEKK4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016:SF32">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19431">
        <property type="entry name" value="MEKK4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="1339"/>
            <end position="1597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="426"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1149"/>
            <end position="1227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1241"/>
            <end position="1270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="453"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1186"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="1248"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="1368"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00060115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00069057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005028941.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1604" mass="181159" checksum="8BF7019B65388885" modified="2023-09-13" version="2">MREAAAAAELVPPPASAVTPAAAMEEPPPPPPLEPEPEPESERCRSARQECTLGDSACKNSESDPEDFPDETSTENLYGASPPSTPRQMKRMSTKHQRNNVGRPAGRSNLKEKMNASNQPPHKDTGKTMENVEEYSYKQEKKIRAALRTTERDHKKNVQCSFMLDSVGGSLPKKSIPDVDLNKPYLSLGCSNAKLPVSVPMPIARTARQTSRTDCPADRLKFFETLRLLLKLTSVSKKKDREQRGQENTSAFWFNRSNELIWLELQAWHAGRTINDQDLFLYTARQAIPDIINEILTFKVNYESFAFVRNGASFNGTSVEGQCKALHGTRVMGYSTYHEHLQRQRVSFEQVKRIMELLEYIEALYPSLQALQKDYEKYAAKDFQDRVQALCLWLNITKDLNQKLRIMGTVLGIKNLSDIGWPVFEIPSPRPSKGNEPEDEGDDAEGELKELESSTDESEEEQISDPKVPETKQPLDNSFSIQSQDCVPKKLKRLESEDDPVGWGAPDCSTEAGFSRHCLTSIYRPFVDKALKQMGLRKLILRLHKLMDGSLQRARIALVKSDRPVEFSEFPDPMWGSDYVQLSRTPPSSEQKCSTVSWDELRSMDLPSFEPAFLVLCRVLLNVIHECLKLRLEQRPAGEPSLLSIKQLVRECKEVLKGGLLMKQYYQFMLHEVLHDLEKTDCNIDAFEEDLHKMLMVYFDYMRSWIQMLQQLPQASHSLKNLLEEEWNFTKEITHYIRGGEAQAGKLFCDIAGMLLKSTGSFLEFGLQESCAEFWTSADDSSASDEIRRSVIEISRALKELFHEARERASKALGFAKMLRKDLEIAAEFILSAPVRDLLEVLKSKQYVKVQIPGLENLQVFVPDSLAEEKSIILQLLNAAAGKDCSKDSDDVLIDAYLLLTKHSDRTRDSGDSWATWEAQPVKIVPQVETVDTLRSMQVDNVLLVVMQSAHLIIQRKAFQQSIEGLMTLRQEQTSSQPVIAKALQQLKNDALELCNRISDAIDRVDHMFTSEFDAEVDESESVTLQQYYREAMIQGYNFGFEYHKEVVRLMSGEFRQKIGDKYISFARKWMNYVLTKCESGRGTRPRWATQGFDFLQAIEPAFISALPEDDFLSLQALMNECIGHVIGKPHSPVTGLYLAIHRNSPRPVKVPRCHSDPPNPHLIIPTPEGFSTRSVPSDARSHGSPAAAAVAAAAATAGRPGPAGSDSAPPKPISSAHETRGSSVPENDRLASIAAELQFRSLSRHSSPTEERDEPAYPKGDSSGSARRSWELRTLISQTKDTASKQGPIEAIQKSVRLFEEKRYREMRRKNIIGQVCDTPKSYDNVMHVGLRKVTFKWQRGNKIGEGQYGKVYTCISVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSRLGLQEHVIRLYSKQITVAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKDFLSHCLESEPRMRWTASQLLDHSFVKVCTDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MNK3</accession>
    <name>A0A8C4MNK3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">STARD3 N-terminal like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">STARD3NL</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the STARD3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MNK3"/>
    <dbReference type="Ensembl" id="ENSEAST00005027945.2">
        <property type="protein sequence ID" value="ENSEASP00005025740.2"/>
        <property type="gene ID" value="ENSEASG00005017566.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155476"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140284">
        <property type="term" value="C:endoplasmic reticulum-endosome membrane contact site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0030301">
        <property type="term" value="P:cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099044">
        <property type="term" value="P:vesicle tethering to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019498">
        <property type="entry name" value="MENTAL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051869">
        <property type="entry name" value="STARD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46121:SF1">
        <property type="entry name" value="STARD3 N-TERMINAL-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46121">
        <property type="entry name" value="STEROIDOGENIC ACUTE REGULATORY PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10457">
        <property type="entry name" value="MENTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51439">
        <property type="entry name" value="MENTAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="55"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="123"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="MENTAL" evidence="7">
        <location>
            <begin position="48"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025740.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="227" mass="25963" checksum="866E045D57CC2D0A" modified="2023-09-13" version="2">MNPLPQNMENALTGSQSSHASLRDVHSINPTQLMARIESYEGREKKGISDVRRTFCLFVTFDLLFVTLLWIIELNVNGGIENTLEKEVVQYDYYSSYFDIFLLAVFRFKVLILAYAVCRLRHWWAIALTTAVTSAFLLAKVILSKLFSQGAFGYVLPIISFILAWIETWFLDFKVLPQEAEEENRLLIVQDASERAALIPGGLSDGQFYSPPESEAEMRKLRPRKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MP01</accession>
    <name>A0A8C4MP01_EQUAS</name>
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        <recommendedName>
            <fullName evidence="17">FRAS1-related extracellular matrix protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Protein QBRICK</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">FREM1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Extracellular matrix protein that plays a role in epidermal differentiation and is required for epidermal adhesion during embryonic development.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with FREM2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FRAS1 family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005030872.2">
        <property type="protein sequence ID" value="ENSEASP00005028426.2"/>
        <property type="gene ID" value="ENSEASG00005019331.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156990"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097094">
        <property type="term" value="P:craniofacial suture morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000081">
        <property type="entry name" value="FRAS1 related extracellular matrix 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2030:FF:000015">
        <property type="entry name" value="FRAS1-related extracellular matrix protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038081">
        <property type="entry name" value="CalX-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003644">
        <property type="entry name" value="Calx_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039005">
        <property type="entry name" value="CSPG_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045658">
        <property type="entry name" value="FRAS1-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051561">
        <property type="entry name" value="FRAS1_ECM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45739:SF7">
        <property type="entry name" value="FRAS1-RELATED EXTRACELLULAR MATRIX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45739">
        <property type="entry name" value="MATRIX PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16184">
        <property type="entry name" value="Cadherin_3"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03160">
        <property type="entry name" value="Calx-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19309">
        <property type="entry name" value="Frem_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141072">
        <property type="entry name" value="CalX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51854">
        <property type="entry name" value="CSPG"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0084">Basement membrane</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0430">Lectin</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 21" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040449684" description="FRAS1-related extracellular matrix protein 1" evidence="21">
        <location>
            <begin position="24"/>
            <end position="2181"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="19">
        <location>
            <begin position="298"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="19">
        <location>
            <begin position="415"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="19">
        <location>
            <begin position="523"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="19">
        <location>
            <begin position="644"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="19">
        <location>
            <begin position="778"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="19">
        <location>
            <begin position="889"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="19">
        <location>
            <begin position="1026"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="19">
        <location>
            <begin position="1149"/>
            <end position="1256"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="19">
        <location>
            <begin position="1277"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="19">
        <location>
            <begin position="1395"/>
            <end position="1487"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="19">
        <location>
            <begin position="1508"/>
            <end position="1598"/>
        </location>
    </feature>
    <feature type="repeat" description="CSPG" evidence="19">
        <location>
            <begin position="1630"/>
            <end position="1726"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="22">
        <location>
            <begin position="2062"/>
            <end position="2169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1872"/>
            <end position="1938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="1879"/>
            <end position="1892"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00058451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00065340"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00074560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00081243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005028426.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="2181" mass="243986" checksum="55B3125102ED86C6" modified="2023-09-13" version="2">MSSLSWGGLNALLLLLLLLPGWASPTFVSTNRGVRVVKGSSAFLSGDDLKFAIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYVHNGCPLLHEDTVKLRLYRFTETDTFMETFILRVYLLEPDCNIIRMSNNVLEVSEFYGLSRAIDKNLLRFDYDRTASLECTVRLDPLGTLLPAHGQMVLVEPQPGEPRGDQPQSFFPESQLGTELKCPGGSCTLGLKKIGSLKVSCEEFLLMGLRYQHMDPPSPNIDYIAIQLDLTDSRSKIVYKSESAWLPVYIRAGIPNQIPRAAFMAMFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPPQGYVTHLWDHTRPVSSFTWKDLSDLQIAYQPPNSSHSERRHYEVELEVYDFFFERSAPIAVHISIRTADTNAPRVSWNTGLNLLEGQSRAITWEQFQIVDNDDIRAVRLVTVDGLQHGRLTLRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDGHHSIRHKFPINILPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDMDSSDDYIFFNITKPPQAGEIMKKPGPGLIGYPVPGFLQKDLFNGIIYYRHFGGEIFEDSFEFVLWDSHEPPNLSVPQVVTIHITPVDDQLPKEAPGVSRHLVVKETEVAYITKKHLHFIDMESYDRELLYTITMPPFFSFSHRHSDAGKLFMVDSIPKLTKNPAAPGLRSFTQHAVNYMKVAYMPPMQDIGPHLQRVQFAFSVSNQHGGTLHGICFNITILPVDNQVPEVFTNPLRVAEGGQCIVSTEHILVSDVDTQLDSIYLSLQRPLPHGRVELNGFPLNTGGTFSWGDLHTLKVRYQHDGSEVLQDDIHLEVTDGTNSAEFVLHVEVLPVNDEPPILKADLTPMMHCSEGNEVIITSEYIFATDIDSDDLKLMFVIARGPQHGVVRRAGVTVDQFSQGDVISGAVTYKHTGGEIGLVPCFDTITLVVSDGGAGPFVNGCCYNGPQPSVPVHDSFPVYDLNITVHPVDNQPPSVAIGTMFVVDEGFSAALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENTLPSAGFEKSNMGISIASFQWKDMKALHINYVQSRHLRMEPTADQFMVYVTDGKQRSLETTFYVIINPTNDEAPDFVVQNFTVCEGQMKELDSSIISAADLDIPKDPLLFSITHKPRHGFLINGVFSKDFPRSKQPANLDQKHELIHNFSMELLQNGMRLIYVHDDSESLADDFTIQLSDGKHKILKTILVEVIPVNDEKPMLSKKAEIVMNMGETRVIPSAVLSAIDGDSPREKIYYLFERLPQNGQLQLKIGRDWVPLYPGLNCTQEEVDLNLLRYTHTGAVASQSQDSFTFSLWDGNNRSPAFDCHIIINDMGKGDIVILARPLVVSKGDRGWLTSATLLAVDGADKPEELLYVITSPPRHGRVEYARHPGVPITSFSQMDIAGRTVCYVHNSKAAVSSDTFRFTVSNGLQTKPGVFEITLETVDRALPVVTRNKGLRLAEGATGLLSPHLLQLTDPDTPAQNLTFLLAQLPQHGQLYLWGTVLLQHNFTQQDVDSGNVAYRHLGGDSQIDRFTFVATDGTNQGFMVDGRVWQEPVSFTIQVDQLDKTAPHITHLHSPSQVGLLKNGCYGIYITSHVLKASDPDTEDNQIIFKILRGPQHGHLENTTTGEFIQEKFSQKDLNSKTILYIINPSLEINSDVMEFQIMDPTGNSATPQILELKWSHVEWSQTEYEICENMGMLPLEITRRGYSMDSAFVSVKVNQVSATVGKDFTLTSSKLIQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKAAVKILDSKGGRCHPSYSFNPNKHSAWEKGSWRPLSPESSSLTNSGSSHLERRRLPSSKQLAVTRGDTLQGFDSTNPPTMKLRTRGNGKTVHPSSVYRNGTDIIYHYHGIVSLKLEDDSSPTHRRKAKVSIISQPQKTVKVAELPQADKVESTTDSHFPRQDQLPSFPKNCTLELEGLFHFEESLQKLYKCDGVAWKAWSSQTKGVEAKSCPAGWHHHSGYCHSLITEPKGTWTTAARACREHHLGSLVTVLSRQHMRWLWSISGRKSFWIGLNDRARAGHWEWIGGETVTFTNWRRGPPQHSKSGKNCVLVQRQGKWQTKNCRKGKRHNYVCSRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MPV9</accession>
    <name>A0A8C4MPV9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">B-cell CLL/lymphoma 6 member B protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Bcl6-associated zinc finger protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">BCL6B</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Associates with BCL6 through the BTB domain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014716960.1">
        <property type="nucleotide sequence ID" value="XM_014861474.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MPV9"/>
    <dbReference type="SMR" id="A0A8C4MPV9"/>
    <dbReference type="Ensembl" id="ENSEAST00005028518.2">
        <property type="protein sequence ID" value="ENSEASP00005026262.2"/>
        <property type="gene ID" value="ENSEASG00005017913.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106844166"/>
    <dbReference type="KEGG" id="eai:106844166"/>
    <dbReference type="CTD" id="255877"/>
    <dbReference type="GeneTree" id="ENSGT00940000159844"/>
    <dbReference type="OrthoDB" id="5560627at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000097">
        <property type="entry name" value="B-cell CLL/lymphoma 6 member B protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000065">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000105">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000289">
        <property type="entry name" value="B-cell CLL/lymphoma 6, member B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001344">
        <property type="entry name" value="Zinc finger protein 16 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF25">
        <property type="entry name" value="ZGC:110075 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="12">
        <location>
            <begin position="43"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="329"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="357"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="385"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="413"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="441"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="147"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="215"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="152"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="167"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="231"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00065558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026262.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="480" mass="51375" checksum="4AF42EEA73E5D0E6" modified="2023-09-13" version="2">MACVAMGSTATAEGALGYVREFTRHSSDVLGNLNELRLRGILTDVTLLVGGQPLRAHKAVLIACSGFFYSIFRGRAGVGVDVLSLPGGPEAGGFAPLLDFMYTSRLRLSPATAPAVLAAATYLQMEHVVQACHRFIQASYEPLGISLRPMEAEPPTPPTVPPPGSPRRSEGHPDPPTESRSCSQGPPSPGSPDPKACNWKKYKFIVLNSQTSQAGSLAGEKSSGQLCPQAGLPSGDEASSSSSSSEEGPIPGPQSRLSPTAATLQFKCGAPINTSHLLAPRAPETTGSPSGQARPPPGSEFFSCQNCEAVAGCSSGLDPLAPGDQDKPYKCQLCRSAFRYKGNLASHRTVHTGEKPYHCSICGARFNRPANLKTHSRIHSGEKPYKCETCGSRFVQVAHLRAHVLIHTGEKPYPCPTCGTRFRHLQTLKSHVRIHTGEKPYHCDPCGLHFRHKSQLRLHLRQKHGAATNTKVHYHILGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MR50</accession>
    <name>A0A8C4MR50_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Repulsive guidance molecule BMP co-receptor b</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RGMB</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the repulsive guidance molecule (RGM) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044635996.1">
        <property type="nucleotide sequence ID" value="XM_044780061.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070373800.1">
        <property type="nucleotide sequence ID" value="XM_070517699.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070373801.1">
        <property type="nucleotide sequence ID" value="XM_070517700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MR50"/>
    <dbReference type="Ensembl" id="ENSEAST00005029991.2">
        <property type="protein sequence ID" value="ENSEASP00005027615.1"/>
        <property type="gene ID" value="ENSEASG00005018795.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106847723"/>
    <dbReference type="CTD" id="285704"/>
    <dbReference type="GeneTree" id="ENSGT00950000183112"/>
    <dbReference type="OMA" id="CHEKMLV"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015026">
        <property type="term" value="F:coreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010604">
        <property type="term" value="P:positive regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1000.10:FF:000001">
        <property type="entry name" value="Repulsive guidance molecule BMP co-receptor a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1000.10">
        <property type="entry name" value="Mog1/PsbP, alpha/beta/alpha sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040287">
        <property type="entry name" value="RGM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009496">
        <property type="entry name" value="RGM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010536">
        <property type="entry name" value="RGM_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31428:SF5">
        <property type="entry name" value="REPULSIVE GUIDANCE MOLECULE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31428">
        <property type="entry name" value="RGM DOMAIN FAMILY MEMBER DRAG-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06534">
        <property type="entry name" value="RGM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06535">
        <property type="entry name" value="RGM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Repulsive guidance molecule N-terminal" evidence="13">
        <location>
            <begin position="97"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Repulsive guidance molecule C-terminal" evidence="12">
        <location>
            <begin position="202"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="164"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="164"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF06535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005027615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="480" mass="51981" checksum="4233FD309A0C1788" modified="2022-01-19" version="1">MMRKKRKRGASPGPCRSHGPGPATAPAPPPSPEPTRSAWTGMGLRAAPSCAAAASAAAAGAEQRRRPRLCPPPLALLLLLLSLGLLHAGDCQQPAQCRIQKCTTDFVSLTSHLNSAIDGFDSEFCKALRAYAGCTQRTSKACRGNLVYHSAVLGISDLMSQRNCSKDGPTSSTNPEVTHDPCNYHSHTGAREHRGGDQTPPNYLFCGLFGDPHLRTFKDHFQTCKVEGAWPLIDNNYLSVQVTNVPVVSGSSATATNKITIIFKAHRECTDQKVYQAVTDDLPAAFVDGSTSGGDGDTKSLRIVERESGRYVEMHARYIGTTVFVRQLGRYLTLAIRMPEDLAMSYEESQDLQLCVNGCPLGERIDDGQGQVSAILGHTLPRTSLTQAWPGYTLETANAQCHEKMPVKDIYFQSCVFDLLTTGDANFTAAAHSALEDVEALHPRKERWHIFPSSSRGTPRGGSRLSVSLGLTCLILIVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MTW4</accession>
    <name>A0A8C4MTW4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Fibulin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">FBLN1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homomultimerizes and interacts with various extracellular matrix components.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Secreted</location>
            <location evidence="1 12">Extracellular space</location>
            <location evidence="1 12">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the fibulin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MTW4"/>
    <dbReference type="Ensembl" id="ENSEAST00005034703.2">
        <property type="protein sequence ID" value="ENSEASP00005031869.2"/>
        <property type="gene ID" value="ENSEASG00005021752.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156642"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016504">
        <property type="term" value="F:peptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00017">
        <property type="entry name" value="ANATO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000341">
        <property type="entry name" value="Fibulin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000078">
        <property type="entry name" value="Fibulin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000104">
        <property type="entry name" value="Fibulin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000108">
        <property type="entry name" value="Fibulin-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000139">
        <property type="entry name" value="Fibulin-1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.10.25.10:FF:000010">
        <property type="entry name" value="Pro-epidermal growth factor"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="9"/>
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    <dbReference type="InterPro" id="IPR000020">
        <property type="entry name" value="Anaphylatoxin/fibulin"/>
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        <property type="entry name" value="cEGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
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    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
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    <dbReference type="InterPro" id="IPR017048">
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        <property type="entry name" value="Fibulin_C"/>
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        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
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    <dbReference type="InterPro" id="IPR052235">
        <property type="entry name" value="Nephronectin_domain"/>
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    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
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    <dbReference type="PANTHER" id="PTHR24050:SF29">
        <property type="entry name" value="FIBULIN-1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR24050">
        <property type="entry name" value="PA14 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01821">
        <property type="entry name" value="ANATO"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="cEGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
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    <dbReference type="Pfam" id="PF22914">
        <property type="entry name" value="Fibulin_C"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
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        <property type="match status" value="8"/>
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        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01177">
        <property type="entry name" value="ANAPHYLATOXIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ANAPHYLATOXIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
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    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="4"/>
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    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 13" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="5 12" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040480726" description="Fibulin-1" evidence="14">
        <location>
            <begin position="33"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphylatoxin-like" evidence="15">
        <location>
            <begin position="39"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Anaphylatoxin-like" evidence="15">
        <location>
            <begin position="80"/>
            <end position="113"/>
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    <feature type="domain" description="Anaphylatoxin-like" evidence="15">
        <location>
            <begin position="115"/>
            <end position="147"/>
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        <location>
            <begin position="362"/>
            <end position="404"/>
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    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="405"/>
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    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="447"/>
            <end position="486"/>
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    <feature type="domain" description="EGF-like" evidence="16">
        <location>
            <begin position="487"/>
            <end position="530"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
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            <dbReference type="ARBA" id="ARBA00006127"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00021554"/>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="709" mass="78033" checksum="C6CFBABDB7D726B5" modified="2023-09-13" version="2">MERAPPSPPPAPLPLPLLLLGGLSLLAARAGADVPLEACCADGHRMATQHRDCSLPYASESKECRMVQEQCCHNQLEELHCATGINLANEQDSCTTPYSDNASLEAMFVKRCCHCCLLGRAAQAQGQSCEYNLMVGYQCGLVFRACCVKGQETADFAPGDVGDLQETAKISEVKEEQEDPYLNDRCRGGGPCKQQCRDTGEEVVCSCFVGYQLLPDGVSCEDINECITGSHNCRLGENCINTVGSFRCQRESSCGTGYELTENNDCKDIDECESGIHNCLPDFICQNTLGSFRCRPKLQCKSGFIQDALGNCIDINECLSISAPCPVGQTCINTEGSYTCQKNVPNCGRGYHLNDEGTRCVDVDECSPPAEPCGPGHRCVNSPGSFRCECKAGYYFDGISRTCIDINECRRYPGRLCGHKCENTPGSYFCSCTVGFRLLADGRSCEDMNECNSSPCSQECANVYGSYQCYCRRGYQLSDVDGVTCEDIDECALPTGGHICSYRCINFPGSFQCSCPSSGYRLAPNGRNCQDIDECVTGIHNCSVNETCFNIQGGFRCLAFECPENYRRSADTLRQEKTDTVRCIKSCRPNDVTCVLDPVHTISHTVVSLPTFREFTRPEEIIFLRAITPAYPANHADIIFDITEGNLRDSFDIIKRYMDGMTVGVVRQVRPIVGPFHAVLKLEMNYVVGGVVSHRNVVNVHIFVSEYWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MU46</accession>
    <name>A0A8C4MU46_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">NEK1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4MU46"/>
    <dbReference type="Ensembl" id="ENSEAST00005034266.2">
        <property type="protein sequence ID" value="ENSEASP00005031526.2"/>
        <property type="gene ID" value="ENSEASG00005021225.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158460"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000242">
        <property type="term" value="C:pericentriolar material"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd08218">
        <property type="entry name" value="STKc_Nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000097">
        <property type="entry name" value="Probable serine/threonine-protein kinase nek1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000172">
        <property type="entry name" value="serine/threonine-protein kinase Nek1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051131">
        <property type="entry name" value="NEK_Ser/Thr_kinase_NIMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899">
        <property type="entry name" value="CAMK FAMILY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44899:SF4">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE NEK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="4"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="332"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="562"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="628"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="850"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1100"/>
            <end position="1154"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="585"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="570"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="850"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010886"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
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            <dbReference type="ARBA" id="ARBA00022527"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00047899"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005031526.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1242" mass="140671" checksum="D4468B216997E3EC" modified="2023-09-13" version="2">MEKYVRLQKIGEGSFGKAVLVKSTEDERQYVIKEINISRMSNKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTIQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIISGSFPPVSLHYSYDLRSLLSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGAQPIPAKRPASGQSLASVVPAPKITKPAAKYGVPLTYKKYGDKKLHEKKPLQKHKQAHQTPVKKVNPGEERRKMFEEPARKGRLEFIEKEKKQKEQISLIKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFFGSGGAVAPSPFPSRGQYEHYHAIFDQMQQQRAQNNEAKWKGETRGRRLPERQKGQLAVERAKQVEEFLQRKREARQNKARAEGHMGILQNLAAMYGGRSSSSRGGKPRNKEEEVYLARLRQIRLQNFNERQQIRAKLRGEKAEADSSEGQEGSEDADTRRKKVEALKAQASARAAVLKEQLERKRKEAYEREKKVWEEHLVAKGVKSSDMSPPSGQHEAGGSPSKQQMRSVISVTSALKEVGMDGSVTDTQETSEEMQKTNNAISSKREILRRLNQHLKAQEDETGKQCRPDTCEIIVHEEAKEHEKEKSVSSDRKKWEAGGQLVIPLDELTLDTSFSATEKHTVGEVIKLDPGGSPRRVWGKSPTNSVLKILGEAELQLQTELLEITAVGSEISPDGENYKPFITGEEKVKSISLEMNPLATVDSSVETNSPKFNEASPQTSLKSEGNLDEPDDLETEVLQEPSGVNKDGSLPCTVIDVWISEIKETKETDRLEDRINIQQNEVSEDGILRNLDQLSEVHMEPGIDDSQHSKCDVDKSMQPEPFFHKMVHSEHLNVVSQIQSILCSPEESFPLRSRSDSPPKSKNKNSLLIGLSTGLFDANNPKMLRTCSLPDLSKLFGTLMDVPTVGDVRQDNLEIDEIEDEHVKDGPSDSEDIVFEETDTDLQELQASMEQLLREQPGEEDSEEEESVLKNSDIEQTANGTELADEDDNPSSESALNEEWHSDNSDGEITSECEYDSVFNHLEELRLHLEQEIGFEKFFEVYEKIKAIHEDEDENIEISSTVVQNILGNEHQHLYAKILHLVMADGAYQEDNDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MWQ6</accession>
    <name>A0A8C4MWQ6_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cysteine rich secretory protein LCCL domain containing 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CRISPLD1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
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        <property type="nucleotide sequence ID" value="XM_014853744.3"/>
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    <dbReference type="Ensembl" id="ENSEAST00005032722.2">
        <property type="protein sequence ID" value="ENSEASP00005030107.2"/>
        <property type="gene ID" value="ENSEASG00005020269.2"/>
    </dbReference>
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    <dbReference type="KEGG" id="eai:106839179"/>
    <dbReference type="CTD" id="83690"/>
    <dbReference type="GeneTree" id="ENSGT00940000156473"/>
    <dbReference type="OrthoDB" id="414826at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060325">
        <property type="term" value="P:face morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="CAP_CRISPLD1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cysteine-rich secretory protein LCCL domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.130.20:FF:000001">
        <property type="entry name" value="Cysteine-rich secretory protein LCCL domain-containing 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.20">
        <property type="entry name" value="LCCL-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018244">
        <property type="entry name" value="Allrgn_V5/Tpx1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051957">
        <property type="entry name" value="CRISP-LCCL_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004043">
        <property type="entry name" value="LCCL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036609">
        <property type="entry name" value="LCCL_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31331:SF9">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN LCCL DOMAIN-CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31331">
        <property type="entry name" value="LCCL DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G08630)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03815">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00603">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69848">
        <property type="entry name" value="LCCL domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01010">
        <property type="entry name" value="CRISP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50820">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="LCCL" evidence="6">
        <location>
            <begin position="289"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="LCCL" evidence="6">
        <location>
            <begin position="390"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="500" mass="56773" checksum="63D83068E156AA61" modified="2023-09-13" version="2">MKCTAREWLRVTTVLFMARAIPAMVVPNATLLEKLLEKYMDEDGEWWTAKQRGKRAITNDDMQSILDLHNKLRSQVYPTASNMEYMTWDVELERSAESWAENCLWEHGPSSLLPSIGQNLGAHWGRYRPPTFHVQAWYDEVKDFSYPYEHECNPYCPFRCSGPVCTHYTQVVWATSSRIGCAINLCHNMNIWGQIWPKAVYLVCNYSPKGNWWGHAPYKHGRPCSACPPSFGGGCRENLCYKEGSDRYYTPQEEETNEIERQQSQVHDAHVRAIADDSNRNEVISTQQMSQIVSCEIRLRDQCKGTTCNRYECPAGCLDSKAKVIGSVHYEMQSSICRAAIHYGIIDNDGGWVDITRQGRKHYFIKSKRNGVQTVGKYQSANSFTVSKVTVQAVTCETTVEQLCPFHKPASHCPRVYCPRNCMQANPHYARVIGTRVYSDLSSICRAAVHAGVVQNQGGYVDVMPVDKRKTYTASFQNGIFSESLQNPPGGKAFRVFAVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MXR3</accession>
    <name>A0A8C4MXR3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ankyrin repeat and SOCS box containing 18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ASB18</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
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    <dbReference type="AlphaFoldDB" id="A0A8C4MXR3"/>
    <dbReference type="Ensembl" id="ENSEAST00005032044.2">
        <property type="protein sequence ID" value="ENSEASP00005029485.2"/>
        <property type="gene ID" value="ENSEASG00005020036.2"/>
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        <property type="component" value="Chromosome 19"/>
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        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd03723">
        <property type="entry name" value="SOCS_ASB4_ASB18"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
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    <dbReference type="Gene3D" id="1.10.750.20">
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        <property type="entry name" value="Ankyrin_rpt"/>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
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    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
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        <property type="entry name" value="SOCS_box-like_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR24118:SF76">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 18"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR24118">
        <property type="entry name" value="POTE ANKYRIN DOMAIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
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    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
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    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
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        <property type="entry name" value="Ankyrin repeat"/>
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        <property type="entry name" value="SOCS box-like"/>
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        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="ANK_REPEAT"/>
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    </dbReference>
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        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="80"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="112"/>
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            <begin position="179"/>
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    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="253"/>
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        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="3">
        <location>
            <begin position="366"/>
            <end position="418"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
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    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005029485.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
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    <sequence length="427" mass="45873" checksum="AC957688B2B62893" modified="2023-09-13" version="2">MTMASADCLPDYPLDSDLVKRLKSALDASDEERVSDLLCTEVEQVDAIIEMANDDWMKDPSAQLPPGVLLGLWTLEYKRELTTPLCIAAARGHTACVSHLLARGADPDASPGGRGALHEACLGGHTACAQLLLQHRADPDLLSAEGLAPLHLCRTAASLGCAQALLEHGASVQRAGGSGGDTPLHVAAQHGLDEHARLYLGRGAPVDARNGRGETALGAACGAARWPDEHARCLRLCALLLRRGAAVDPRDEDERSPLHQACGRARPDLASLLLRHGADAGALDYGGASPLARALQTAACAPRAAAPRTVQALLNYGSPTVWPDAFPKVLKTCASAPEVVEVLFNSYPQLRVSESWKEAIPEEVFEMHQPFYKSFFALAHTPRCLQHLCRCAIRKLFGKNCFYFIPLLPLPKSLQNYLLLEPEGVLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N0F4</accession>
    <name>A0A8C4N0F4_EQUAS</name>
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        <recommendedName>
            <fullName evidence="17">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="17">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">SRMS</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Non-receptor tyrosine-protein kinase which phosphorylates DOK1 on tyrosine residues. Also phosphorylates KHDRBS1/SAM68 and VIM on tyrosine residues. Phosphorylation of KHDRBS1 is EGF-dependent. Phosphorylates OTUB1, promoting deubiquitination of RPTOR.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 17">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts (via the SH2 and SH3 domains) with DOK1. Interacts with KHDRBS1/SAM68 and VIM.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein kinase superfamily. Tyr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="17"/>
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    <dbReference type="Ensembl" id="ENSEAST00005036932.2">
        <property type="protein sequence ID" value="ENSEASP00005033881.2"/>
        <property type="gene ID" value="ENSEASG00005023170.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161518"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031669">
        <property type="term" value="P:cellular response to nutrient levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000458">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000053">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.505.10:FF:000078">
        <property type="entry name" value="Tyrosine-protein kinase"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050198">
        <property type="entry name" value="Non-receptor_tyrosine_kinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24418">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014697409.1">
        <property type="nucleotide sequence ID" value="XM_014841923.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PFD9"/>
    <dbReference type="Ensembl" id="ENSEAST00005001035.2">
        <property type="protein sequence ID" value="ENSEASP00005000897.1"/>
        <property type="gene ID" value="ENSEASG00005000776.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831622"/>
    <dbReference type="KEGG" id="eai:106831622"/>
    <dbReference type="GeneTree" id="ENSGT01150000286905"/>
    <dbReference type="OMA" id="PNMLLGF"/>
    <dbReference type="OrthoDB" id="5969463at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000013">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF126">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="103"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="145"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="46"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="339" mass="37747" checksum="30550D35687F5CD8" modified="2022-01-19" version="1">MGEDGNTSTFNISYTNFFLVGFPGLHERRPLLVLPLTFLYVTIISANGLVIHTVVGQRNLHQPMYMLIALLLAVNICAATAVMPKMLEGFVHYANPISLHGCLAQMFFIYFTLLLDYNLLLAMALDRYMAICHPLRYTDLMTSRLLGMLAILALTRSLGVAVPLVVLTSRAQFCRTAVIRHFTCEYIALLSIACGDLTFNNRLGLAMRLVTVTFDLALLGTSYTRIIYVAFRISSGKARAKALHTCGSHFLVILTIYLSGLSTSIVFRVTKTVSQDVQNLLSAIYLLLPGALNPVIYGVRTREIQEHVEKMLCGKESAQEVREKPKRLQNRRVDGKLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PFF8</accession>
    <name>A0A8C4PFF8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ferritin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Delivery to lysosomes is mediated by the cargo receptor NCOA4 for autophagic degradation and release of iron.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited. Interacts with NCOA4; NCOA4 promotes targeting of the iron-binding ferritin complex to autolysosomes following starvation or iron depletion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the ferritin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PFF8"/>
    <dbReference type="Ensembl" id="ENSEAST00005001170.2">
        <property type="protein sequence ID" value="ENSEASP00005001020.2"/>
        <property type="gene ID" value="ENSEASG00005000860.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182841"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01056">
        <property type="entry name" value="Euk_Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.10:FF:000024">
        <property type="entry name" value="Ferritin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431:SF37">
        <property type="entry name" value="FERRITIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="4 15" id="KW-0409">Iron storage</keyword>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="16">
        <location>
            <begin position="11"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <property type="term" value="P:determination of pancreatic left/right asymmetry"/>
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        <property type="term" value="P:epithelial cilium movement involved in determination of left/right asymmetry"/>
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        <property type="term" value="P:flagellated sperm motility"/>
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        <property type="term" value="P:heart looping"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:inner dynein arm assembly"/>
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        <property type="term" value="P:protein localization to cilium"/>
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        <property type="entry name" value="CCDC40"/>
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        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 40"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <begin position="252"/>
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            <begin position="434"/>
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        <location>
            <begin position="518"/>
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        <location>
            <begin position="686"/>
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        <location>
            <begin position="754"/>
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    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="870"/>
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    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1003"/>
            <end position="1062"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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            <begin position="37"/>
            <end position="55"/>
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            <begin position="125"/>
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            <begin position="197"/>
            <end position="206"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PJ44</accession>
    <name>A0A8C4PJ44_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TSPAN2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">May play a role in signalling in oligodendrocytes in the early stages of their terminal differentiation into myelin-forming glia and may also function in stabilizing the mature sheath.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014848244.3"/>
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    <dbReference type="Ensembl" id="ENSEAST00005009606.2">
        <property type="protein sequence ID" value="ENSEASP00005008818.1"/>
        <property type="gene ID" value="ENSEASG00005006328.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835682"/>
    <dbReference type="KEGG" id="eai:106835682"/>
    <dbReference type="CTD" id="10100"/>
    <dbReference type="GeneTree" id="ENSGT00940000157504"/>
    <dbReference type="OMA" id="VQVKPTC"/>
    <dbReference type="OrthoDB" id="5870230at2759"/>
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        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043209">
        <property type="term" value="C:myelin sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014002">
        <property type="term" value="P:astrocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014005">
        <property type="term" value="P:microglia development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042552">
        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048709">
        <property type="term" value="P:oligodendrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03151">
        <property type="entry name" value="CD81_like_LEL"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF155">
        <property type="entry name" value="TETRASPANIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="221" mass="24305" checksum="220DD4ED48277F05" modified="2022-01-19" version="1">MGRFRGGLRCIKYLLLGFNLLFWLAGSAVIAFGLWFRFGGTMKDFSSEDKSPEYFYMGLYVLVGAGALMMAVGFFGCCGATRESQCVLGSFFTCLLVIFAAEVTTGVFAFIGKDVAIRHVQTMYEEAYNGYLRDREKGNGTLITFHSTFQCCGKESSEQVQPTCPKELLGHKNCINEIETTISVKLQLIGIVGIGIAGFTILGMIFSMVLCCAIRNSRDVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PJ46</accession>
    <name>A0A8C4PJ46_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tropomodulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PLE3"/>
    <dbReference type="Ensembl" id="ENSEAST00005015620.2">
        <property type="protein sequence ID" value="ENSEASP00005014376.1"/>
        <property type="gene ID" value="ENSEASG00005010009.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160631"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005865">
        <property type="term" value="C:striated muscle thin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030239">
        <property type="term" value="P:myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051694">
        <property type="term" value="P:pointed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000006">
        <property type="entry name" value="Tropomodulin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004934">
        <property type="entry name" value="TMOD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901">
        <property type="entry name" value="TROPOMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901:SF15">
        <property type="entry name" value="TROPOMODULIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03250">
        <property type="entry name" value="Tropomodulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="281" mass="31867" checksum="04F469761748B4EC" modified="2022-01-19" version="1">MALPFQKELEKYKNIDEDELLGKLSEEELKQLENVLDDLDPESAMLPAGFRQKDQTQKAATGPFDREHLLMYLEKEALEQKDREDFVPFTGEKKDVVKGEKVKPVFEEPPNPTNVEVSLQQMKANDPSLQEVNLNNIKSIPIPTLKEFAKALETNTHVKKFSLAATRSNDPVAVAFANMLKVNKTLKSLNIESNFITGTGILALVEALRENDTLTEIKIDNQRQQLGTAVEMEIAQMLEENSRILKFGYQFTKQGPRTRVAAAITKNNDLVRKKRVEGDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PLP7</accession>
    <name>A0A8C4PLP7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="23 26">Anti-Muellerian hormone type-2 receptor</fullName>
            <ecNumber evidence="3 26">2.7.11.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24 26">Anti-Muellerian hormone type II receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25 26">MIS type II receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">AMHR2</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21 26">On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for anti-Muellerian hormone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[receptor-protein] + ATP = O-phospho-L-seryl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18673"/>
            <dbReference type="Rhea" id="RHEA-COMP:11022"/>
            <dbReference type="Rhea" id="RHEA-COMP:11023"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[receptor-protein] + ATP = O-phospho-L-threonyl-[receptor-protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:44880"/>
            <dbReference type="Rhea" id="RHEA-COMP:11024"/>
            <dbReference type="Rhea" id="RHEA-COMP:11025"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.30"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="26">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="26">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="22">Interacts with type I receptor ACVR1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 26">Membrane</location>
            <topology evidence="1 26">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 26">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.30" evidence="3 26"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4PLP7"/>
    <dbReference type="Ensembl" id="ENSEAST00005015646.2">
        <property type="protein sequence ID" value="ENSEASP00005014401.2"/>
        <property type="gene ID" value="ENSEASG00005010020.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160885"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990272">
        <property type="term" value="F:anti-Mullerian hormone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042562">
        <property type="term" value="F:hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005026">
        <property type="term" value="F:transforming growth factor beta receptor activity, type II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007548">
        <property type="term" value="P:sex differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd23616">
        <property type="entry name" value="TFP_LU_ECD_AMHR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000487">
        <property type="entry name" value="Anti-Muellerian hormone type-2 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000012">
        <property type="entry name" value="Anti-Muellerian hormone type-2 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000349">
        <property type="entry name" value="Anti-Muellerian hormone type-2 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015771">
        <property type="entry name" value="Anti-muellerian_hrmn_rcpt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000333">
        <property type="entry name" value="TGFB_receptor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255:SF49">
        <property type="entry name" value="ANTI-MUELLERIAN HORMONE TYPE-2 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23255">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA RECEPTOR TYPE I AND II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037392">
        <property type="entry name" value="AMHRII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 26" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 26" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 26" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18 26" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 26 28" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 26" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 26" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16 26" id="KW-0675">Receptor</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 26" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 29" id="KW-0732">Signal</keyword>
    <keyword evidence="5 26" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 28" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="29">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040130762" description="Anti-Muellerian hormone type-2 receptor" evidence="29">
        <location>
            <begin position="19"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="146"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="30">
        <location>
            <begin position="204"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00057747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00065680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00072617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00075133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00083687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="PIRNR" id="PIRNR037392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014401.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="541" mass="58861" checksum="0DD5E384DDF8E671" modified="2023-09-13" version="2">MMLGTLGLWALLPTAVQAPPSTRTCVFFEAPGVRGSTKTLGKLLDAGPGPPRVIRCLYSRCCFGIWNLTQDRAQVEMQGCRDSDEPGCESPDCDPSPRAHPSPSSTLFTCSCGTDFCNANYSHLPPPGSPGTLGSQGLQAVAGESIWMALVLLGLFLLLLLLLGSIILVLLQRKASRVRGGPEPEPEPDSGRDWSTELPELPELCFSQVIREGGHAVVWAGQLQGELVAIKAFPLRAVAQFQAERALYELPGLQHDHIVRFITASRGGPGPPSCGPLLVLELHPKGSLCHYLTQHTSDWGSSLRMALSLAQGLAFLHEERWQDGQYKPGIAHRDLSSQNVLIREDGSCAIGDLGLALVLPGLTQPPAWAPTQPQGPAAIMEAGTQRYMAPELLDKTLDLQDWGMALRRADVYSLALLLWEILSRCLDLRPDSRPPPFQLAYEAELGSTPTTCELWTLAVEERRRPYIPSTWHCFATDPGGLRELLEDCWDADPEARLTAECVQQRLAALAQPQEAHSFPEGCPPPCPEDCLSTPPHCHSPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PLW0</accession>
    <name>A0A8C4PLW0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Gap junction protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">GJD4</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">A connexon is composed of a hexamer of connexins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 9">Cell membrane</location>
            <topology evidence="2 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the connexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PLW0"/>
    <dbReference type="Ensembl" id="ENSEAST00005016080.2">
        <property type="protein sequence ID" value="ENSEASP00005014786.1"/>
        <property type="gene ID" value="ENSEASG00005010318.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286949"/>
    <dbReference type="OMA" id="CTRPPCT"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005243">
        <property type="term" value="F:gap junction channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019570">
        <property type="entry name" value="Connexin_CCC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984">
        <property type="entry name" value="CONNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11984:SF3">
        <property type="entry name" value="GAP JUNCTION DELTA-4 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01089">
        <property type="entry name" value="Connexin_CCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00408">
        <property type="entry name" value="CONNEXINS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0303">Gap junction</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin N-terminal" evidence="12">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Connexin cysteine-rich" evidence="13">
        <location>
            <begin position="131"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="204"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="292"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="354" mass="38178" checksum="944BF9C90F283A2B" modified="2022-01-19" version="1">MERLDLLGFLIVTLNCNVTIVGKIWLLLTMLLRMAVVVLAGAPVYQDEQERFVCNTLQPGCANVCYDVFSPVSHLRFWLIQSVSVLLPSAVFSVYVLHRGVVLAARGEARNLDVPDFSCGYMVHLFLRTLTEAAFGALHYLLFGFLVPKRFSCTRPPCTSVVDCYVSRPTEKSIMMLFLWAVSVLSLLLSVADLACSARRRMRRGSGRGCGAARAPRGQGLAGGRSPGHVARAGEWEGGAVPALPARWAGGEGAHSPTGRSVSGLMELPDEDESEAMSLASDKPARAGPEPGSRHHGEASQDLRSEGLAGSEEPPPAPRSRLARRCLSSPLQPPARPGPAGSVPVLRTRRSEWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PM15</accession>
    <name>A0A8C4PM15_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">OSBPL9</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070366035.1">
        <property type="nucleotide sequence ID" value="XM_070509934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PM15"/>
    <dbReference type="Ensembl" id="ENSEAST00005017126.2">
        <property type="protein sequence ID" value="ENSEASP00005015747.2"/>
        <property type="gene ID" value="ENSEASG00005010962.2"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00940000154690"/>
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        <property type="component" value="Chromosome 5"/>
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        <property type="term" value="C:cytosol"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:sterol binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Oxysterol-binding protein"/>
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    </dbReference>
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        <property type="entry name" value="Oxysterol-binding protein"/>
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        <property type="entry name" value="Oxysterol-binding protein-like"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3 6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="112"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="584"/>
            <end position="611"/>
        </location>
    </feature>
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        <location>
            <begin position="156"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
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            <begin position="217"/>
            <end position="232"/>
        </location>
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        <location>
            <begin position="239"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023055"/>
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            <dbReference type="ARBA" id="ARBA00023121"/>
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    </evidence>
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            <dbReference type="RuleBase" id="RU003844"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU003845"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="639" mass="71507" checksum="DA324835F6EE0942" modified="2023-09-13" version="2">MAFLLATCGGLDSGFVPSVQDFDKKLTEADAYLQILIEQLKLFDDKLQNCKDDEQRKKIETLKETTNSMVESIKHCIVLLQIAKDQCNAEKHADGIISTINPVDAIYQPSPLEPVINTMPSQTALPPEPAQLCKSEQRPSSLPVGPVLATLGHHQTPTPNSTGSGHSPPSSSLTSPSHVNLSPNTVPEFSYSSSEDEFYDADEFHQSGSSPKRLIDSSGSASVLTHSSSGNSLKRPDTTESLNSSISNGTSDADLFDSHDDRDDEGEAGSVEEHKSVIMHLLSQVRLGMDLTKVVLPTFILERRSLLEMYADFFAHPDLFVSISDQKDARDRMVQVVKWYLSAFHAGRKGSVAKKPYNPILGEIFQCHWTLPNDTEENVELVAEGPVPWVSKNSVTFVAEQVSHHPPISAFYAECFNKKIQFNAHIWTKSKFLGMSIGVHNIGQGCVSCLDHDEHYILTFPNGYGRSILTVPWVELGGECNISCSKTGYSANIVFHTKPFYGGKKHRITAEIFSPNDKKSFCSIEGEWNGVMYAKYATGENAVFIDTKKLPIIKKKVRKLEDQNEYESRCLWKDVTFNLKIRDIDAATEAKHRLEERQRAEARERKEKEIQWETRLFHEDGECWVYDEPLLKRLGAAKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PPM5</accession>
    <name>A0A8C4PPM5_EQUAS</name>
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        <recommendedName>
            <fullName evidence="2">Leucine-rich repeat-containing protein 42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LRRC42</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LRRC42 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PPM5"/>
    <dbReference type="Ensembl" id="ENSEAST00005023096.2">
        <property type="protein sequence ID" value="ENSEASP00005021272.2"/>
        <property type="gene ID" value="ENSEASG00005014568.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002727"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039631">
        <property type="entry name" value="LRRC42"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31994">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31994:SF3">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="394"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005021272.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="428" mass="48277" checksum="6C338555A4124398" modified="2023-09-13" version="2">MSCYLNSENHLDPGPIYVRENGQLHMVNLALDGVRSSLQKPRPFRLFPKGFSVELCMNREDDTAQKEKTDHFIFTYTREGNLRYSAKSLFSLVLGFISDNVDHIDSLIGFPEQIAEKLFSAAEARQKFTEPGAGLRALQKFTEAYGSLVLCSLCLRNRYLVISEKLEEIKSFRELTCLDLSCCKLGDEHELLEHLTNEALSSVTQLHLKDNCLSDAGVRKMTAPVRVMKRGLENLTLLDLSCNPEITDAGIGYLFSFRKLNCLDISGTGIKDITAIKHKLQTHIGLVHSKVPLKEFDHSNCKTEGWADQIVLQWERVTSEAVKLQETSEPRAAAQRFYGKRARTEAPGKCPLADTHMNSSEKLQFCKEKAADCHGPLLKCEAVLSQESKKSKKRAFEEPEKEQSNSSQSSKQKYVCLAVEDWDLLNSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PPX1</accession>
    <name>A0A8C4PPX1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 20">protein kinase C</fullName>
            <ecNumber evidence="4 20">2.7.11.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">PRKCD</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
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                <person name="S."/>
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                <person name="Y."/>
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                <person name="Q."/>
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                <person name="Wang"/>
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                <person name="Guo"/>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="4 20"/>
    <dbReference type="RefSeq" id="XP_044610519.1">
        <property type="nucleotide sequence ID" value="XM_044754584.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044610524.1">
        <property type="nucleotide sequence ID" value="XM_044754589.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070348845.1">
        <property type="nucleotide sequence ID" value="XM_070492744.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PPX1"/>
    <dbReference type="Ensembl" id="ENSEAST00005023757.2">
        <property type="protein sequence ID" value="ENSEASP00005021896.2"/>
        <property type="gene ID" value="ENSEASG00005014952.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822994"/>
    <dbReference type="KEGG" id="eai:106822994"/>
    <dbReference type="CTD" id="5580"/>
    <dbReference type="GeneTree" id="ENSGT00940000155327"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036019">
        <property type="term" value="C:endolysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004699">
        <property type="term" value="F:diacylglycerol-dependent, calcium-independent serine/threonine kinase activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043560">
        <property type="term" value="F:insulin receptor substrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030296">
        <property type="term" value="F:protein tyrosine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042100">
        <property type="term" value="P:B cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904385">
        <property type="term" value="P:cellular response to angiotensin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070301">
        <property type="term" value="P:cellular response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071447">
        <property type="term" value="P:cellular response to hydroperoxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034644">
        <property type="term" value="P:cellular response to UV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090398">
        <property type="term" value="P:cellular senescence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016064">
        <property type="term" value="P:immunoglobulin mediated immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030837">
        <property type="term" value="P:negative regulation of actin filament polymerization"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051490">
        <property type="term" value="P:negative regulation of filopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034351">
        <property type="term" value="P:negative regulation of glial cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050728">
        <property type="term" value="P:negative regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046627">
        <property type="term" value="P:negative regulation of insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090331">
        <property type="term" value="P:negative regulation of platelet aggregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042119">
        <property type="term" value="P:neutrophil activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141212">
        <property type="term" value="P:phospholipase C/protein kinase C signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001235">
        <property type="term" value="P:positive regulation of apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000304">
        <property type="term" value="P:positive regulation of ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000753">
        <property type="term" value="P:positive regulation of glucosylceramide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042307">
        <property type="term" value="P:positive regulation of protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000755">
        <property type="term" value="P:positive regulation of sphingomyelin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032930">
        <property type="term" value="P:positive regulation of superoxide anion generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043687">
        <property type="term" value="P:post-translational protein modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070528">
        <property type="term" value="P:protein kinase C signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023021">
        <property type="term" value="P:termination of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd20834">
        <property type="entry name" value="C1_nPKC_theta-like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20837">
        <property type="entry name" value="C1_nPKC_theta-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000360">
        <property type="entry name" value="Protein kinase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000003">
        <property type="entry name" value="Protein kinase C delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000049">
        <property type="entry name" value="Protein kinase C delta type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000008">
        <property type="entry name" value="Protein kinase C theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000150">
        <property type="entry name" value="Protein kinase C, theta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027436">
        <property type="entry name" value="PKC_delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014376">
        <property type="entry name" value="Prot_kin_PKC_delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24351">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21494">
        <property type="entry name" value="PKC_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000551">
        <property type="entry name" value="PKC_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF501104">
        <property type="entry name" value="Protein_kin_C_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16 20 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 20 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 20" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="9 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2" evidence="25">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="27">
        <location>
            <begin position="158"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="27">
        <location>
            <begin position="230"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="26">
        <location>
            <begin position="376"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="28">
        <location>
            <begin position="631"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="321"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="21">
        <location>
            <position position="500"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <begin position="382"/>
            <end position="390"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="22 23">
        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRNR" id="PIRNR000551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000551-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR000551-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005021896.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="703" mass="80465" checksum="226BEB6E46B556EB" modified="2023-09-13" version="1">MAPFLRIAFNSYELGSLQAEDEASQPFCAVKMKEALSTDRGKTLVQKKPTMYPEWKSTFDAHIYEGRVIQIVLMRAAEEPVSEVTVGVSVLAERCKKNNGKAEFWLDLQPQAKVLMSVQYFLEDIDCKQSVRAEDEAKFPTMNRRGAIKQAKIHYIKNHEFIATFFRQPTFCSVCKDFVWGLNKQGYKCRQCNAAIHKKCIDKIIGRCTGTAANSRDTIFQKERFNIDMPHRFKVYNYMSPTFCDHCGSLLWGLVKQGLKCEDCGMNVHHKCQKKVANLCGINQKLLAEALNQVSQRPTRKSESGPPEHFGIYQNFEKKPGVSGDDAPGEAKAHFSQESPISPQGLRMEPSLPPLSNHSTYGKIWEGSNRCNIENFTFHKVLGKGSFGKVLLAELKGKKEFFAIKALKKDVVLIDDDVECTMVEKRVLALAWENPFLTHLLCTFQTKDHLFFVMEFLNGGDLMYHIQDKGRFELYRATFYAAEIICGLQFLHNKGIIYRDLKLDNVMLDQDGHIKIADFGMCKENIFGEKQASTFCGTPDYIAPEILQGLKYSFSVDWWSFGVLLYEMLIGQSPFHGDDEDELFESIRMDTPHYPRWITKESKDILEKLLERDPTKRLGVTGNIKAHSFFKTINWTLLEKRAVEPPFKPKVKSPGDYSNFDQEFLNEKPRLSYSDKNLIDSMDQAAFAGFSFVNPKFERLLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IAG2</accession>
    <name>A0A9L0IAG2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="4">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MINDY2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Hydrolase that can specifically remove 'Lys-48'-linked conjugated ubiquitin from proteins. Has exodeubiquitinase activity and has a preference for long polyubiquitin chains. May play a regulatory role at the level of protein turnover.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the MINDY deubiquitinase family. FAM63 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0IAG2"/>
    <dbReference type="Ensembl" id="ENSEAST00005041193.1">
        <property type="protein sequence ID" value="ENSEASP00005037146.1"/>
        <property type="gene ID" value="ENSEASG00005006799.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016607"/>
    <dbReference type="OMA" id="WKTENTP"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016807">
        <property type="term" value="F:cysteine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140934">
        <property type="term" value="F:histone deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071795">
        <property type="term" value="F:K11-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990380">
        <property type="term" value="F:K48-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036435">
        <property type="term" value="F:K48-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071796">
        <property type="term" value="F:K6-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007518">
        <property type="entry name" value="MINDY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033979">
        <property type="entry name" value="MINDY_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18063">
        <property type="entry name" value="NF-E2 INDUCIBLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18063:SF8">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE MINDY-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04424">
        <property type="entry name" value="MINDY_DUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="MINDY deubiquitinase" evidence="6">
        <location>
            <begin position="250"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="562"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="55"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="566"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="605"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005037146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="629" mass="67934" checksum="96B0726157AC861A" modified="2023-09-13" version="1">MESGPESLQPLENGVAAVPAPGTGSPQEGRQETRLAAGDGPGVWAAECDGGNGQGAAAARRSLPKSGSPAGSPPIPGPCSSFSGSDLKESDLASPAAQKALLGGQHKVTASPETAEAGAGHGLGPAGDAGARPDPTGTCRAESAAAGSEEPSSAGGLSSSCSDPSPPGESPSLDSLESFSNLHSFPSSSEFNSEEGAENRVPEEEEEEEGAAVLPGAVPLCKGEEEGEEEEGEAAQVLAASKERFPGQSVYHIKWIQWKEENTPIITQNENGPCPLLAILNVLLLAWKVKLPPMMEIITAEQLMEYLGDYMLDAKPKEISEIQRLNYEQNMSDAMAILHKLQTGLDVNVKFTGVRVFEYTPECIVFDLLDIPLYHGWLVDPQIDDIVKAVGNCSYNQLVEKIISCKQSDNSELVSEGFVAEQFLNNTATQLTYHGLCELTSTVQEGELCVFFRNNHFSTMTKYKGLLYLLVTDQGFLTEEKVVWESLHNVDGDGNFCDSEFHLRPPSDPETVYRGQQDQIDQDYLMALSLQQEQQSQEINWEQIPEGISDLELAKKLQEEEDRRASQYYQEQEQAAAAAASAATQAQPSQPAQASPSSGRQSGNSERKRKEPREKDKEKEKEKNSCVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IB97</accession>
    <name>A0A9L0IB97_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 12">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="4 12">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">UGDH</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the formation of UDP-alpha-D-glucuronate, a constituent of complex glycosaminoglycans. Required for the biosynthesis of chondroitin sulfate and heparan sulfate. Required for embryonic development via its role in the biosynthesis of glycosaminoglycans. Required for proper brain and neuronal development.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>UDP-alpha-D-glucose + 2 NAD(+) + H2O = UDP-alpha-D-glucuronate + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
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    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
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        <text evidence="3">Homohexamer.</text>
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        <text evidence="2 12">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
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            <begin position="20"/>
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<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the adaptor protein complex 4 (AP-4). Adaptor protein complexes are vesicle coat components involved both in vesicle formation and cargo selection. They control the vesicular transport of proteins in different trafficking pathways. AP-4 forms a non clathrin-associated coat on vesicles departing the trans-Golgi network (TGN) and may be involved in the targeting of proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system. It is also involved in protein sorting to the basolateral membrane in epithelial cells and the proper asymmetric localization of somatodendritic proteins in neurons. AP-4 is involved in the recognition and binding of tyrosine-based sorting signals found in the cytoplasmic part of cargos, but may also recognize other types of sorting signal.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Adaptor protein complex 4 (AP-4) is a heterotetramer composed of two large adaptins (epsilon-type subunit AP4E1 and beta-type subunit AP4B1), a medium adaptin (mu-type subunit AP4M1) and a small adaptin (sigma-type AP4S1).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014857403.3"/>
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        <property type="nucleotide sequence ID" value="XM_014857412.3"/>
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        <property type="nucleotide sequence ID" value="XM_044765449.2"/>
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        <property type="nucleotide sequence ID" value="XM_044765450.2"/>
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        <property type="entry name" value="NOELIN"/>
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        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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        </source>
    </evidence>
    <sequence length="510" mass="58053" checksum="F08B9784BD93D3E6" modified="2023-09-13" version="1">MSVPLLKIGVVLSTMAMITNWMSQTLPSLVGLNTTKLSAASGGTLDRSTGVLPTNPEESWQVYSSAQDSEGRCICTVVAPQQTMCSRDARTKQLRQLLEKVQNMSQSIEVLDRRTQRDLQYVEKMENQMKGLESKFKQVEESHKQHLARQFKAIKAKMDELRPLIPVLEEYKADAKLVLQFKEEVQNLTSVLNELQEEIGAYDYDELQSRVSNLEERLRACMQKLACGKLTGISDPVTVKTSGSRFGSWMTDPLAPEGDNRVWYMDGYHNNRFVREYKSMIDFMTTDNFTSHRLPHPWSGTGQVVYNGSIYFNKFQSHIVIRFDLKTETILKTRSLDYAGYNNMYHYAWGGHSDIDLMVDENGLWAVYATNQNAGNIVISKLDPDLLAGAADLEHKLPQAQCRGSLHHLRDPLRHQRLLGGHQGPLRLPDQRLDLRVHRHPLPEQVLPHLHVGLQPQGPRPVRVEQRPPDPLQRHPLPCHPVRRVVVASARKPRVRGLPHVEIPVKQLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IFW9</accession>
    <name>A0A9L0IFW9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106845698</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014719553.1">
        <property type="nucleotide sequence ID" value="XM_014864067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IFW9"/>
    <dbReference type="Ensembl" id="ENSEAST00005052541.1">
        <property type="protein sequence ID" value="ENSEASP00005036670.1"/>
        <property type="gene ID" value="ENSEASG00005029729.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106845698"/>
    <dbReference type="KEGG" id="eai:106845698"/>
    <dbReference type="GeneTree" id="ENSGT01120000271834"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000004">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="196"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="237"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005036670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="309" mass="35262" checksum="A02975D46EDDE54C" modified="2023-09-13" version="1">MEMENATLLTEFILSGLIYQHKWHIPLFLVFLVIYFTTIVGNLVLIALVWNDPQLHIPMYLFLGSLAFVDACLSSAVTSKMLVNFFATNKMISFSECLIQIFFFGIGVTTECFLLAAMAYDRYVAICQPLLYPVIMTNRLCIRLLVLSFFGGLLNVTTHVGLIFRLTFCKYNIIHHFYCDIMPLFKISCTDPSINFLMVFIFAGSIQVFTILTVLVSYTLVLFTILKRKSVQGFRKAFSTCGAHLLSVPLYYGPLAFRYVRPGSAQADDQDMVDSLFYTIIIPLLNPIIYSLRNKKVIDSLVKMLKRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IG80</accession>
    <name>A0A9L0IG80_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Geranylgeranyl transferase type-2 subunit beta</fullName>
            <ecNumber evidence="3 13">2.5.1.60</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">RABGGTB</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of Rab proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX, such as RAB1A, RAB3A, RAB5A and RAB7A.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Catalyzes the transfer of a geranylgeranyl moiety from geranylgeranyl diphosphate to both cysteines of proteins with the C-terminal sequence -XXCC, -XCXC and -CCXX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 13">
            <text>geranylgeranyl diphosphate + L-cysteinyl-[protein] = S-geranylgeranyl-L-cysteinyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11537"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:86021"/>
            <dbReference type="EC" id="2.5.1.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotrimer composed of RABGGTA, RABGGTB and CHM; within this trimer, RABGGTA and RABGGTB form the catalytic component B, while CHM (component A) mediates peptide substrate binding. The Rab GGTase dimer (RGGT) interacts with CHM (component A) prior to Rab protein binding; the association is stabilized by geranylgeranyl pyrophosphate (GGpp). The CHM:RGGT:Rab complex is destabilized by GGpp. Interaction of RABGGTB with prenylated PTP4A2 precludes its association with RABGGTA and inhibits enzyme activity. Interacts with CHODL. Interacts with non-phosphorylated form of RAB8A; phosphorylation of RAB8A at 'Thr-72' disrupts this interaction.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.60" evidence="3 13"/>
    <dbReference type="RefSeq" id="XP_014704990.1">
        <property type="nucleotide sequence ID" value="XM_014849504.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IG80"/>
    <dbReference type="Ensembl" id="ENSEAST00005047953.1">
        <property type="protein sequence ID" value="ENSEASP00005039893.1"/>
        <property type="gene ID" value="ENSEASG00005017201.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106836537"/>
    <dbReference type="KEGG" id="eai:106836537"/>
    <dbReference type="CTD" id="5876"/>
    <dbReference type="GeneTree" id="ENSGT00950000183128"/>
    <dbReference type="OrthoDB" id="5428259at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005968">
        <property type="term" value="C:Rab-protein geranylgeranyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004663">
        <property type="term" value="F:Rab geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02894">
        <property type="entry name" value="GGTase-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000004">
        <property type="entry name" value="Geranylgeranyl transferase type-2 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026873">
        <property type="entry name" value="Ptb1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF11">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE-2 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5 13" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="14">
        <location>
            <begin position="18"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00062019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="331" mass="36978" checksum="13404B00B55B707B" modified="2023-09-13" version="1">MGTPQKDVIIKSDAPDTLLLEKHADYIASYGSKKDDYEYCMSEYLRMSGIYWGLTVMDLMGQLHRMNREEILTFIKSCQHECGGISASIGHDPHLLYTLSAVQILTLYDSINVIDVNKVVEYVQSLQKEDGSFAGDTWGEIDTRFSFCAVATLALLGKLDAINVEKAIEFVLSCMNFDGGFGCRPGSESHAGQIYCCTGFLAITSQLHQVNSDLLGWWLCERQLPSGGLNGRPEKLPDVCYSWWVLASLKIIGRLHWIDREKLRSFILACQDEETGGFADRPGDMVDPFHTLFGIAGLSLLGEEQIKPVSPVFCMPEEVLRRMNVQPELVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IGM4</accession>
    <name>A0A9L0IGM4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Hedgehog-interacting protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">HHIP</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">Interacts with all three hedgehog family members, SHH, IHH and DHH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HHIP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070360937.1">
        <property type="nucleotide sequence ID" value="XM_070504836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IGM4"/>
    <dbReference type="Ensembl" id="ENSEAST00005043665.1">
        <property type="protein sequence ID" value="ENSEASP00005036203.1"/>
        <property type="gene ID" value="ENSEASG00005001609.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106829161"/>
    <dbReference type="CTD" id="64399"/>
    <dbReference type="GeneTree" id="ENSGT00940000158660"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000026">
        <property type="entry name" value="hedgehog-interacting protein-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000020">
        <property type="entry name" value="Latent-transforming growth factor beta-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013111">
        <property type="entry name" value="EGF_extracell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018143">
        <property type="entry name" value="Folate_rcpt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012938">
        <property type="entry name" value="Glc/Sorbosone_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011041">
        <property type="entry name" value="Quinoprot_gluc/sorb_DH_b-prop"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19328">
        <property type="entry name" value="HEDGEHOG-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19328:SF27">
        <property type="entry name" value="HEDGEHOG-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07974">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03024">
        <property type="entry name" value="Folate_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07995">
        <property type="entry name" value="GSDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50952">
        <property type="entry name" value="Soluble quinoprotein glucose dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 16" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 17" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040404179" description="Hedgehog-interacting protein" evidence="17">
        <location>
            <begin position="18"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="18">
        <location>
            <begin position="635"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="639"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="657"/>
            <end position="666"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005036203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="682" mass="76886" checksum="9166FE7E96CF1646" modified="2023-09-13" version="1">MLKMLSFKLLLLAVALGFFEGDAKFGERSEGSGARRRRCLNGNPPKRLKRRDRRMMSQLELLSGGEMLCGAFYPRLSCCLRSDSPGLGRLDNKIFSVTNNTECGKLLEEIKCALCSPHSQSLFHSPEREALERDLELPLLCKDYCKEFFYTCRGHIPGFLQTTADEFCFYYARKDGGLCFPDFPRKQIRGPASNYLDQMEEYDKVEEMSRKHKHNCFCIQEVVSGLRQPVSALHSGDGSHRLFILEKEGYVKILTPEGEIFKEPYLDIHKLVQSGIKGGDERGLLSLAFHPNYKKNGKLYVSYTTNQERWAIGPHDHILRVVEYTVSRKNPHQVDLRTARVFLEVAELHRKHLGGQLLFGPDGFLYIILGDGMITLDDMEEMDGLSDFTGSVLRLDVDTDMCSVPYSIPRSNPHFNSTSQPPEVFAHGLHDPGRCAVDRHPTDININLTILCSDSNGKNRSSARILQIIKGKDYESEPSLLEFKPFSNGPLVGGFVYRGCQSERLYGSYVFGDRNGNFLTLQQSPVSKQWQEKPLCLGNSGSCRGYFSGHILGFGEDELGEVYILSSSKSMTQTHNGKLYKIVDPKRPLMPEECRAMVQPAQTLTSECSRLCRNGYCTPTGKCCCSPGWEGDFCRIAKCEPACRHGGVCVRPNKCLCKKGYLGPQCEQVDRNIRRVTRTSPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IK09</accession>
    <name>A0A9L0IK09_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable E3 SUMO-protein ligase RNF212</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Probable E3 SUMO-protein transferase RNF212</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">RING finger protein 212</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">RNF212</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">SUMO E3 ligase that acts as a regulator of crossing-over during meiosis: required to couple chromosome synapsis to the formation of crossover-specific recombination complexes. Localizes to recombination sites and stabilizes meiosis-specific recombination factors, such as MutS-gamma complex proteins (MSH4 and MSH5) and TEX11. May mediate sumoylation of target proteins MSH4 and/or MSH5, leading to enhance their binding to recombination sites. Acts as a limiting factor for crossover designation and/or reinforcement and plays an antagonist role with CCNB1IP1/HEI10 in the regulation of meiotic recombination.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044623952.1">
        <property type="nucleotide sequence ID" value="XM_044768017.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IK09"/>
    <dbReference type="Ensembl" id="ENSEAST00005065674.1">
        <property type="protein sequence ID" value="ENSEASP00005037393.1"/>
        <property type="gene ID" value="ENSEASG00005036465.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106825433"/>
    <dbReference type="CTD" id="285498"/>
    <dbReference type="GeneTree" id="ENSGT00740000115581"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000795">
        <property type="term" value="C:synaptonemal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019789">
        <property type="term" value="F:SUMO transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051026">
        <property type="term" value="P:chiasma assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006311">
        <property type="term" value="P:meiotic gene conversion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016925">
        <property type="term" value="P:protein sumoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16746">
        <property type="entry name" value="RING-HC_RNF212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000839">
        <property type="entry name" value="Ring finger protein 212"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042123">
        <property type="entry name" value="Zip3/RNF212-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22663:SF21">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE RNF212-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22663">
        <property type="entry name" value="RING FINGER PROTEIN NARYA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14634">
        <property type="entry name" value="zf-RING_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0469">Meiosis</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="11">
        <location>
            <begin position="7"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="164"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00059057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00074434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00080634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005037393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="285" mass="31062" checksum="016C85535A957A79" modified="2023-09-13" version="1">MASWVFCNRCFQPPRQTACFSLTNCGHVYCEGCLGKGKKDECVICKVPCRTVLLSKHTDSDIQALFMGIDGLCKKYSKETSQISEFQEKHRKRLLTFYREKISKLEDSLRKSVLRIQELQSMRSSQQTVFSTIRNSVSTPSAQPHGCPFLPPDSSASERVESMEVDLTPSPLRKPEIAAGPTRISLISPPQDGRMGSVSHGPQHLGLTPGHSSMPQALRIPSLQIPCKGRSHTPDPQAPGRAGSGGSPSLGGPRAPAPRQPISISGLLQRRHAGSAAVRGFAHER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IKM7</accession>
    <name>A0A9L0IKM7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Ectonucleoside triphosphate diphosphohydrolase 5</fullName>
            <ecNumber evidence="15">3.6.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Guanosine-diphosphatase ENTPD5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Uridine-diphosphatase ENTPD5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">ENTPD5</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Hydrolyzes nucleoside diphosphates with a preference for GDP, IDP and UDP compared to ADP and CDP. In the lumen of the endoplasmic reticulum, hydrolyzes UDP that acts as an end-product feedback inhibitor of the UDP-Glc:glycoprotein glucosyltransferases. UMP can be transported back by an UDP-sugar antiporter to the cytosol where it is consumed to regenerate UDP-glucose. Therefore, it positively regulates protein reglucosylation by clearing UDP from the ER lumen and by promoting the regeneration of UDP-glucose. Protein reglucosylation is essential to proper glycoprotein folding and quality control in the ER.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>ADP + H2O = AMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61436"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:61437"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>CDP + H2O = CMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:64880"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="3.6.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:64881"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>GDP + H2O = GMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22156"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:22157"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>IDP + H2O = IMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35207"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58280"/>
            <dbReference type="EC" id="3.6.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:35208"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>UDP + H2O = UMP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:64876"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="3.6.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:64877"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a ribonucleoside 5'-diphosphate + H2O = a ribonucleoside 5'-phosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36799"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.6.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:36800"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Monomer; active form. Homodimer; disulfide-linked. Homodimers are enzymatically inactive.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 29">Belongs to the GDA1/CD39 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.6" evidence="15"/>
    <dbReference type="RefSeq" id="XP_014691148.2">
        <property type="nucleotide sequence ID" value="XM_014835662.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044629921.1">
        <property type="nucleotide sequence ID" value="XM_044773986.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070370135.1">
        <property type="nucleotide sequence ID" value="XM_070514034.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070370136.1">
        <property type="nucleotide sequence ID" value="XM_070514035.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070370137.1">
        <property type="nucleotide sequence ID" value="XM_070514036.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070370138.1">
        <property type="nucleotide sequence ID" value="XM_070514037.1"/>
    </dbReference>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
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                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
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                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="ERp29"/>
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        <property type="entry name" value="ERp29_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036356">
        <property type="entry name" value="ERp29_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012883">
        <property type="entry name" value="ERp29_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12211">
        <property type="entry name" value="ENDOPLASMIC RETICULUM PROTEIN ERP29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12211:SF0">
        <property type="entry name" value="ENDOPLASMIC RETICULUM RESIDENT PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07749">
        <property type="entry name" value="ERp29"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF07912">
        <property type="entry name" value="ERp29_N"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF47933">
        <property type="entry name" value="ERP29 C domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
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        </location>
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    <feature type="chain" id="PRO_5040499019" description="Endoplasmic reticulum resident protein 29" evidence="3">
        <location>
            <begin position="33"/>
            <end position="255"/>
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    </feature>
    <feature type="domain" description="ERp29 N-terminal" evidence="5">
        <location>
            <begin position="33"/>
            <end position="149"/>
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    </feature>
    <feature type="domain" description="Endoplasmic reticulum resident protein 29 C-terminal" evidence="4">
        <location>
            <begin position="150"/>
            <end position="245"/>
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            <dbReference type="Pfam" id="PF07912"/>
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        </source>
    </evidence>
    <sequence length="255" mass="28546" checksum="234AD4D76DFB967A" modified="2023-09-13" version="1">MAAAMSYAASLSPLFPLLLFLLLLSAPHGGSGLHTKGALPLDTVTFYKFVLVKFDTQYPYGEKQDEFKRLAENSASSDDLLVAEVGISDYGDKLNMELSEKYKLDKENYPVFYLFRDGDFENPILYNGVIKVGAIQRWLKGQGVYLGMPGCLPAYDALAGEFIRASGMEARQALLKLGQDNLASVKETEKKWAEQYLKIMGKVLDQGEDFPASEMTRITKLIEKNKMSDGKKEELQKSLNILTAFQKKEAEKEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IM40</accession>
    <name>A0A9L0IM40_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ALF transcription elongation factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">AFF1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AF4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IM40"/>
    <dbReference type="Ensembl" id="ENSEAST00005079263.1">
        <property type="protein sequence ID" value="ENSEASP00005038133.1"/>
        <property type="gene ID" value="ENSEASG00005012760.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182974"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="C:super elongation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="Gene3D" id="6.10.250.2670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007797">
        <property type="entry name" value="AF4/FMR2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043640">
        <property type="entry name" value="AF4/FMR2_CHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043639">
        <property type="entry name" value="AF4_int"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10528">
        <property type="entry name" value="AF4/FMR2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10528:SF6">
        <property type="entry name" value="AF4_FMR2 FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05110">
        <property type="entry name" value="AF-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18875">
        <property type="entry name" value="AF4_int"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18876">
        <property type="entry name" value="AFF4_CHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AF4/FMR2 C-terminal homology" evidence="6">
        <location>
            <begin position="1004"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="59"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="109"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="237"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="412"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1148"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="59"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="115"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="283"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="311"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="439"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="467"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="486"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="498"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="517"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="570"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="589"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="600"/>
            <end position="610"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="5">
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            <begin position="613"/>
            <end position="622"/>
        </location>
    </feature>
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        <location>
            <begin position="703"/>
            <end position="719"/>
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    </feature>
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        <location>
            <begin position="741"/>
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        <location>
            <begin position="782"/>
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        <location>
            <begin position="837"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="869"/>
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    </feature>
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        <location>
            <begin position="974"/>
            <end position="987"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1166"/>
            <end position="1183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF18876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1266" mass="136782" checksum="A513371D91F49BC3" modified="2023-09-13" version="1">MGDVARRTTSKCVRIFPPYGLEIQSASSGQSCLAQNNKHSKLGLYNEDRNLLRIREKERRNQEAHQEKEAFPEKIPLFGEPYKTEKGDELSSRIQNMLGNYEEVKELLSTKSHPHRLDASENRLGKPRYPLFPEKGSSIPSHSFHTSVHHQPVNTPASGPLPVGNISHNPKMAQPRMEPMPSLHVKSYGAPDSQHLPQDRLGQEAYGSTHHKKGDRRADGDHCASMADLAPERELSPLFSSLPSPVPPLSPIHSSQQALPRTQGSTKVHSSNNNNKGYCPAKSPKDLAVKVRDKDPPPDGLVAVASLGVAPPQPPSQTFPPPPLPSKSIAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSVEQTYSNEAHCVEEILKEMTHSWPPPLTAIHTPSTAEPSKFPFPTKDSQHISSATQNQKQYDTSSKTHTNSQQGTSMLEDDLQLSDSEDSDGEQTPEKPPSTSAPPSAPQSLPAASASAHSSSAESESTSDSDSSSDSESESSSSDSEENEPLEAPAPEPEPPTTNKWQLDNWLTKVSQPAAPPEAPGGTEPPPRHLDGKGQGGDGGGPAGREHSDSKDPPPKSSSRAPRVPSEGPHAGKRSSQKSPAHQEPPQRQTVGTKQPKKAGKASGPAEPRAGLQVESEPGAPPRGPAEQPAKDKPRVKTKGRPRAADAGEPPEQAEPRPAAPAAGERRKHRSGPAGPLKVPSGPEPPKDRVEARSPEHFALVPLAQSQGPPRGGGRTSGCRRAVVVQEERRKDKDKLPVPLRDTRLLSPLRDTPAPQSLVVKITLELLSRVPPPPGKGSRPKKPEDRQAPPGKKLDAERRSSDNSGKLAKKRKGEAERDHDSKKVRLEKEIKSQSSSSSSHKESSKTKTPRPSSEPSKKEMLPPQLVSSSSSSSQKPAKPAQKRPRREAEPSGQDPPKSASSAKGSHKDSSVPKHRKVEGKGSESSTEHKGSSGDIANPFPVPSLPNGNSKPGKPQMKFDKQQADFYMKEAKKLKIKAETTTDKVGKAFKYAEAALSLIECGIAMESEGPAAKSAYSVYSETVDLINFVLSLKSFSDVTTPLPGKIFAVLCMRCQSILNMAMFRCKKDTAIKYSRTLNEHFKISSKVAQAPSPCVARSTGTPSPLSPMPSPASSIGSQSSAGSVGSSGMPATISTPVTIQNMTSSYVTITSHILTAFDLWEQAEFLTRNNKEFFAQLSTSERALALNSSLEDLVRYTRQGFQRLKQVTKTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IP73</accession>
    <name>A0A9L0IP73_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Translation machinery-associated protein 16</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="J."/>
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                <person name="S."/>
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                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the biogenesis of the 60S ribosomal subunit in the nucleus.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Associates with pre-60S ribosomal particles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TMA16 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IP73"/>
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        <property type="protein sequence ID" value="ENSEASP00005042766.1"/>
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        <property type="component" value="Chromosome 13"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Translation machinery-associated 16 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.170">
        <property type="entry name" value="Translation machinery-associated protein 16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021346">
        <property type="entry name" value="Tma16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038356">
        <property type="entry name" value="Tma16_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13349:SF2">
        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11176">
        <property type="entry name" value="Tma16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="94"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="97"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="110"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005042766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="214" mass="24541" checksum="B999895401A00348" modified="2023-09-13" version="1">MCDVTQPKAPKGKTVGWEKKVIHPYSRKADQIMREAHRQEKNERLKNEEELRLNLVDEKLQWFQNHFDPQKVGYSKKDACELTDRCLNRFNSEQEQTELHNSIKDRQGGRAVPRRQSASARRSVSNSGYEGYKDACGLEVPDAVNADNLKTLREQDFDLKKLPSTTMRKTCANDALPKKCWKKTVTTVDKDLEELELKDDSALNALDAEMTSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IPY4</accession>
    <name>A0A9L0IPY4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Gamma-glutamylcyclotransferase</fullName>
            <ecNumber evidence="5">4.3.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CHAC2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3 5">Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>glutathione = L-cysteinylglycine + 5-oxo-L-proline</text>
            <dbReference type="Rhea" id="RHEA:47724"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58402"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
            <dbReference type="EC" id="4.3.2.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the gamma-glutamylcyclotransferase family. ChaC subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.7" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0IPY4"/>
    <dbReference type="Ensembl" id="ENSEAST00005043255.1">
        <property type="protein sequence ID" value="ENSEASP00005039841.1"/>
        <property type="gene ID" value="ENSEASG00005024339.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003855"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003839">
        <property type="term" value="F:gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061928">
        <property type="term" value="F:glutathione specific gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.10:FF:000003">
        <property type="entry name" value="Gamma-glutamylcyclotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006840">
        <property type="entry name" value="ChaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192">
        <property type="entry name" value="CATION TRANSPORT PROTEIN CHAC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192:SF2">
        <property type="entry name" value="GLUTATHIONE-SPECIFIC GAMMA-GLUTAMYLCYCLOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04752">
        <property type="entry name" value="ChaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00045227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="172" mass="19694" checksum="508852E8594ED521" modified="2023-09-13" version="1">MWVFGYGSLIWKVDFPYQDKVVGYITGFSRRFWQGSTDHRGIPGKGCVWGVAYRLPVGKEEEVKSYLDFREKGGYRTITVSFYPKESTTEPFTVLLYIGTHDNPNYLGPAPLEDIAEQIFHAAGPSGKNSEYLFELANSMRNLVPEDADEHLFSLEKLVKERLERKQNLSCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IQQ6</accession>
    <name>A0A9L0IQQ6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SH3-containing GRB2-like protein 3-interacting protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Endophilin-3-interacting protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SGIP1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May function in clathrin-mediated endocytosis. Has both a membrane binding/tubulating activity and the ability to recruit proteins essential to the formation of functional clathrin-coated pits. Has a preference for membranes enriched in phosphatidylserine and phosphoinositides and is required for the endocytosis of the transferrin receptor. May also bind tubulin. May play a role in the regulation of energy homeostasis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with proteins essential or regulating the formation of functional clathrin-coated pits. Interacts with CANX. Interacts with AP2A1. Interacts with EPS15. Interacts with SH3GL3. Interacts with AMPH. Interacts with ITSN1 (via SH3 domains). Interacts with and REPS1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <location evidence="2">Clathrin-coated pit</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IQQ6"/>
    <dbReference type="Ensembl" id="ENSEAST00005044726.1">
        <property type="protein sequence ID" value="ENSEASP00005040106.1"/>
        <property type="gene ID" value="ENSEASG00005007954.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156301"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048260">
        <property type="term" value="P:positive regulation of receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd09266">
        <property type="entry name" value="SGIP1_MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1170:FF:000005">
        <property type="entry name" value="SH3-containing GRB2-like protein 3-interacting protein 1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018808">
        <property type="entry name" value="Muniscin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037984">
        <property type="entry name" value="SGIP1_MHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF8">
        <property type="entry name" value="F-BAR DOMAIN ONLY PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10291">
        <property type="entry name" value="muHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0168">Coated pit</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MHD" evidence="11">
        <location>
            <begin position="583"/>
            <end position="851"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="62"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="109"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="170"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="340"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="16"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="272"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="362"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="376"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="402"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="461"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="481"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="525"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003346"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
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            <dbReference type="ARBA" id="ARBA00014150"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00023176"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030485"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005040106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="852" mass="91810" checksum="111C60B8F44B8036" modified="2023-09-13" version="1">MMEGLKKRTRKAFGIRKKEKDTDSTGSPDRDGIQGKKKTQKTQLLLTSCFWLRALSLTLFQPSPHEPPYSNKAECAREGGKKVSKKSNGAPNGFYAEIDWERYNSPELDEEGYSIRPEEPGSTKGKHFYSSSESEEEEESHKKFNIKIKPLQSKDILKHAATVDELKASIGNIALSPSPVRKSPRRSPGAIKRNLSSEEVARPRRSTPTPELISKKPPDDTVALAPLFGPPLESAFDEQKTEVILDQPEIWGSGQPINPSVESPKLTRPFPTGTPPPLPPKNVPATPPRTGSPLTVGPGSDQSATEVKIEKLPSINDLDSIFGPVLSPKSVAVNAEEKWVHFSDTSPEHVTPELSPREKVVSPPAASDNPDDSPAPGSPGPPGPPGPPGPPRSVPSPLNLEEVQKKVTEQTFIKDDYLETISSPKDFGLGQRATPPPPPPPTYRTVVSSPGPGSGSGTGTASGASSPARPATPLVPCSSTTPPPPPPRPPSRPKLPPGKPGVGDVPRPFSPPRHSSSPPPIAPLARAESTSSISSTNSLSAATTPTVENEQPSLVWFDRGKFYLTFEGSSRGPSPLTMGAQDTLPVAAAFTETVNAYFKGADPSKCIVKITGEMVLSFPAGITRHFANNPSPAALTFRVINFSRLEHVLPNPQLLCCDNTQNDANTKEFWVNMPNLMTHLKKVSEQKPQATYYNVDMLKYQVSAQGIQSTPLNLAVNWRCEPSSTDLRIDYKYNTDAMTTAVALNNVQFLVPIDGGVTKLQAVLPPAVWNAEQQRILWKIPDISQKSENGGVGSLLARFQLSEGPSKASPLVVQFTSEGSTLSGCDIELVGAGYRFSLIKKRFAAGKYLADN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IRD7</accession>
    <name>A0A9L0IRD7_EQUAS</name>
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        <recommendedName>
            <fullName evidence="11">GPN-loop GTPase</fullName>
            <ecNumber evidence="11">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GPN1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
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                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
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                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
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                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII). May act at an RNAP assembly step prior to nuclear import. Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding proteins, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. May be involved in nuclear localization of XPA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Binds to RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterodimer with GPN3. Binds to RNA polymerase II (RNAPII). Interacts directly with RNAPII subunits RPB4 and RPB7 and the CTD of RPB1. Interacts with XPA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0IRD7"/>
    <dbReference type="Ensembl" id="ENSEAST00005070598.1">
        <property type="protein sequence ID" value="ENSEASP00005043449.1"/>
        <property type="gene ID" value="ENSEASG00005009082.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183172"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17870">
        <property type="entry name" value="GPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000696">
        <property type="entry name" value="GPN-loop GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030230">
        <property type="entry name" value="Gpn1/Npa3/XAB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF8">
        <property type="entry name" value="GPN-LOOP GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    <sequence length="267" mass="29352" checksum="09084C50C66D2479" modified="2023-09-13" version="1">MAAPAAAAEPQASGGPRPLMCLLVLGMAGSGKTTFVQRLTGHLHSQGSPPYVINLDPAVHEVPFPANIDIRDTVKYKEVMKQYGLGPNGGIVTSLNLFATRFDQVMKFIEKAQNISKYVLIDTPGQIEVFTWSASGTIITEALASSFPTIVIYVMDTSRSTNPVTFMSNMLYACSILYKTKLPFIVVMNKTDIIDHSFAVEWMQDFEAFQDALNQETTYVSNLTRSMSLVLDEFYSSLRVVGVSAVLGTGLDEFFVQVTSAAEEYER</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IV30</accession>
    <name>A0A9L0IV30_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myostatin</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
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                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
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                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IV30"/>
    <dbReference type="Ensembl" id="ENSEAST00005070621.1">
        <property type="protein sequence ID" value="ENSEASP00005041621.1"/>
        <property type="gene ID" value="ENSEASG00005038681.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040444184" description="Myostatin" evidence="2">
        <location>
            <begin position="20"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005041621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="56" mass="6139" checksum="B69B161BD1709045" modified="2023-09-13" version="1">MLALTRILLLILQMQVVTELKTEQDPNCSEPDVEGVSPPAVGAQTPMDADKQAIYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IWI6</accession>
    <name>A0A9L0IWI6_EQUAS</name>
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        <recommendedName>
            <fullName evidence="4">NTF2-related export protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">NXT1</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Has a role in nuclear-cytoplasmic transport of proteins and mRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
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    <dbReference type="RefSeq" id="XP_044604382.1">
        <property type="nucleotide sequence ID" value="XM_044748447.2"/>
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    <dbReference type="AlphaFoldDB" id="A0A9L0IWI6"/>
    <dbReference type="SMR" id="A0A9L0IWI6"/>
    <dbReference type="Ensembl" id="ENSEAST00005046212.1">
        <property type="protein sequence ID" value="ENSEASP00005037574.1"/>
        <property type="gene ID" value="ENSEASG00005033641.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005054995.1">
        <property type="protein sequence ID" value="ENSEASP00005045249.1"/>
        <property type="gene ID" value="ENSEASG00005033641.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005056893.1">
        <property type="protein sequence ID" value="ENSEASP00005046376.1"/>
        <property type="gene ID" value="ENSEASG00005033641.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005062205.1">
        <property type="protein sequence ID" value="ENSEASP00005053964.1"/>
        <property type="gene ID" value="ENSEASG00005033641.1"/>
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        <property type="component" value="Chromosome 15"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00780">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.50:FF:000006">
        <property type="entry name" value="NTF2-related export protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045875">
        <property type="entry name" value="NTF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032710">
        <property type="entry name" value="NTF2-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002075">
        <property type="entry name" value="NTF2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018222">
        <property type="entry name" value="Nuclear_transport_factor_2_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12612">
        <property type="entry name" value="NUCLEAR TRANSPORT FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02136">
        <property type="entry name" value="NTF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54427">
        <property type="entry name" value="NTF2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50177">
        <property type="entry name" value="NTF2_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NTF2" evidence="5">
        <location>
            <begin position="16"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU369002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50177"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005045249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
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            <fullName evidence="20">NAD(P)H oxidoreductase RTN4IP1, mitochondrial</fullName>
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        <alternativeName>
            <fullName evidence="21">Reticulon-4-interacting protein 1</fullName>
        </alternativeName>
    </protein>
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        <name evidence="23" type="primary">RTN4IP1</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">NAD(P)H oxidoreductase involved in the ubiquinone biosynthetic pathway. Required for the O-methyltransferase activity of COQ3. Able to catalyze the oxidoreduction of 3-demethylubiquinone into 3-demethylubiquinol in vitro. However, it is unclear if 3-demethylubiquinone constitutes a substrate in vivo. May also play a role in the regulation of retinal ganglion cell (RGC) neurite outgrowth, and hence in the development of the inner retina and optic nerve. Appears to be a potent inhibitor of regeneration following spinal cord injury.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>3-demethylubiquinone-10 + NADH + 2 H(+) = 3-demethylubiquinol-10 + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:83243"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64182"/>
            <dbReference type="ChEBI" id="CHEBI:231824"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>3-demethylubiquinone-10 + NADPH + 2 H(+) = 3-demethylubiquinol-10 + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:83247"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:64182"/>
            <dbReference type="ChEBI" id="CHEBI:231824"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 3-demethylubiquinone + NADH + 2 H(+) = a 3-demethylubiquinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:83235"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="Rhea" id="RHEA-COMP:19654"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="ChEBI" id="CHEBI:231825"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a 3-demethylubiquinone + NADPH + 2 H(+) = a 3-demethylubiquinol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:83239"/>
            <dbReference type="Rhea" id="RHEA-COMP:10914"/>
            <dbReference type="Rhea" id="RHEA-COMP:19654"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:84422"/>
            <dbReference type="ChEBI" id="CHEBI:231825"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; ubiquinone biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with RTN4, UQCRC1 and UQCRC2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014716312.1">
        <property type="nucleotide sequence ID" value="XM_014860826.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IXD4"/>
    <dbReference type="Ensembl" id="ENSEAST00005042688.1">
        <property type="protein sequence ID" value="ENSEASP00005045641.1"/>
        <property type="gene ID" value="ENSEASG00005006435.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106843686"/>
    <dbReference type="CTD" id="84816"/>
    <dbReference type="GeneTree" id="ENSGT00880000138028"/>
    <dbReference type="OMA" id="TSWQALK"/>
    <dbReference type="OrthoDB" id="48317at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
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                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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    <sequence length="226" mass="24484" checksum="0AEAA2311BD60997" modified="2023-09-13" version="1">MSFSESGRCSVPLRRRLGTPVPLARPAFPAFTHGGGWGEGEVDEEEGCDQVARDLRAEFSAGASSEPRKGSLLPRDGDGSPVLPDKRNGIFSAEAGGRARARRWPVQVLSILCSLLFAILLAFLLAIAYLVVKELHAENLKKEADVDTGLLGFWTLLIISLTAGFSCCSFSWTVTYFDSFEPGMFPPTPLSPARFKKLTGHSFHMGYSMAILNGIVAALTVAWCLM</sequence>
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                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
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                <person name="Jordana"/>
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                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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        <text evidence="13">Cytochromes P450 are a group of heme-thiolate monooxygenases.</text>
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    <comment type="catalytic activity">
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            <text>an organic molecule + reduced [NADPH--hemoprotein reductase] + O2 = an alcohol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:142491"/>
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    </comment>
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        <cofactor evidence="1 11 13">
            <name>heme</name>
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        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Microsome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.1" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0J6L5"/>
    <dbReference type="Ensembl" id="ENSEAST00005058333.1">
        <property type="protein sequence ID" value="ENSEASP00005045652.1"/>
        <property type="gene ID" value="ENSEASG00005004861.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161670"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008392">
        <property type="term" value="F:arachidonate epoxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019373">
        <property type="term" value="P:epoxygenase P450 pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20670">
        <property type="entry name" value="CYP2G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000001">
        <property type="entry name" value="Cytochrome P450, family 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008067">
        <property type="entry name" value="Cyt_P450_E_grp-I_CYP2A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF424">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01684">
        <property type="entry name" value="EP450ICYP2A"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 11 12" id="KW-0349">Heme</keyword>
    <keyword evidence="8 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 13" id="KW-0492">Microsome</keyword>
    <keyword evidence="9 12" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="11">
        <location>
            <position position="428"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU368053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005045652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="483" mass="55809" checksum="0FBC8952F57EDA97" modified="2023-09-13" version="1">QILEFISSWSIILQMSKIRLVEVWGVLPRITKSLCLFLNSLSNTMTHLYLREKYGPVFTVYMGPRPVVVLCGHEAVKEALVDQADNFSGRGELATIERNFQGHGLAMANGERWRILRRFSLTILRDFGMGKRSIEERIQEEAGFLLKELRKTKGAPIDPTFFLSRTVSNVISAIVFGSRFDYEDKQFQKLMQMINESFTEMSTPWAQLYDMYSGIMQYLPGRHNHIYYLIEELKDFIASRVKINEASLDPQNPRDFIDCFLIKMHQDKSNPYTEFNFKNLVLTTLNLFFAGTETVSSTLRYGLLLMMKYPEVEAKIHEEIEQVIGSRRIPSVDDRVKMPYTDAVIHEIQRLTDIVPMGVPHNVIRDTHFRGYFLPKGTDVFPLLGSVLKDPKYFRYPDAFYPQHFLDDQGHFKKNEAFVPFSSGKRICLGEALARMELFLYFTSILQNFSLRSLVPPADIDITPKVSGFGNIPPTYELCFMVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J9F8</accession>
    <name>A0A9L0J9F8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">SH3 domain-binding glutamic acid-rich-like protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SH3BGRL3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Could act as a modulator of glutaredoxin biological activity. May play a role in cytoskeleton organization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Ruffle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SH3BGR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J9F8"/>
    <dbReference type="Ensembl" id="ENSEAST00005051347.1">
        <property type="protein sequence ID" value="ENSEASP00005046642.1"/>
        <property type="gene ID" value="ENSEASG00005038458.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157260"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd03030">
        <property type="entry name" value="GRX_SH3BGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000132">
        <property type="entry name" value="SH3 domain-binding glutamic acid-rich-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006993">
        <property type="entry name" value="Glut_rich_SH3-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051033">
        <property type="entry name" value="SH3BGR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12232">
        <property type="entry name" value="SH3 DOMAIN-BINDING GLUTAMIC ACID-RICH-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12232:SF3">
        <property type="entry name" value="SH3 DOMAIN-BINDING GLUTAMIC ACID-RICH-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04908">
        <property type="entry name" value="SH3BGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040125652" description="SH3 domain-binding glutamic acid-rich-like protein 3" evidence="15">
        <location>
            <begin position="31"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="85"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00040886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005046642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="297" mass="32492" checksum="C9C0440A3B7979D7" modified="2023-09-13" version="1">VERKCGENLLSGAVPVLVCLLSCGVSHVWVSPEGVGRGSVERGSLAWELQVWKQHKPSKPSCATCSRGSEVGSGVQQTRARLRSRLLPNLRPCPPGLSRHGVSRQPQTVSPRSGEGRRGRYCEGLGSWARPAPEREGGRRRAGRGRSSHAPAARLPAPPIGPESSDSRAEQEGNRSRARRRRLQAVQLPLPPPVRLSARPSTRSMSGLRVYSTSVTGSREIKSQQSEVTRILDGKRIQYQLVDISQDNALRDEMRALAGNPKATPPQIVNGDQYCGDYELFVEAVEQNTLQEFLKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J9U1</accession>
    <name>A0A9L0J9U1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Solute carrier family 35 member C1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SLC35C1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Putative transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TPT transporter family. SLC35E subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044605708.1">
        <property type="nucleotide sequence ID" value="XM_044749773.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J9U1"/>
    <dbReference type="Ensembl" id="ENSEAST00005070510.1">
        <property type="protein sequence ID" value="ENSEASP00005050061.1"/>
        <property type="gene ID" value="ENSEASG00005014922.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106843984"/>
    <dbReference type="KEGG" id="eai:106843984"/>
    <dbReference type="CTD" id="55343"/>
    <dbReference type="GeneTree" id="ENSGT00390000013315"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005457">
        <property type="term" value="F:GDP-fucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042351">
        <property type="term" value="P:'de novo' GDP-L-fucose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0036085">
        <property type="term" value="P:GDP-fucose import into Golgi lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0042352">
        <property type="term" value="P:GDP-L-fucose salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="213"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="311"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="9">
        <location>
            <begin position="85"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="25"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00093775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="409" mass="44370" checksum="189EA9D491F81E5A" modified="2023-09-13" version="1">MGRGRRGWHGAGARGREQEVVSRGRGPGRGAAGAALGGAGGRPVRSRRAPLKRSRILHMALMGASDPTGEAEASEEKPFLLRAVQIALVVSLYWVTSISMVFLNKYLLNSPSLRLDTPIFVTFYQCLVTTLLCKGLSALAACCPGAVDFPSLRLDLRVARSILPLSVVFIGMITFNNLCLKYVGVAFYNVGRSLTTVFNVLLSYLLLKQTTSFYALLACGIIIGGFWLGVDQEGAEGTLSWTGTVFGVLASLCVSLNAIYTKKVLPAVDGSIWRLTFYNNVNACVLFLPLLLLLGELQTLRNFSQLGSAHFWGMMTLGGLFGFAIGYVTGLQIKFTSPLTHNVSGTAKACAQTVLAVLYYEDTKSFLWWTSNMMVLGGSSAYTWVRGWEMKKVQEEPSPKEEEKSGVGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JCU7</accession>
    <name>A0A9L0JCU7_EQUAS</name>
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        <recommendedName>
            <fullName evidence="15">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106843448</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
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    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Cell membrane</location>
            <topology evidence="2 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JCU7"/>
    <dbReference type="Ensembl" id="ENSEAST00005070141.1">
        <property type="protein sequence ID" value="ENSEASP00005051131.1"/>
        <property type="gene ID" value="ENSEASG00005033654.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106843448"/>
    <dbReference type="KEGG" id="eai:106843448"/>
    <dbReference type="GeneTree" id="ENSGT00940000163392"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0004930">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15911">
        <property type="entry name" value="7tmA_OR11A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000010">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF378">
        <property type="entry name" value="OLFACTORY RECEPTOR 11A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="92"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="201"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="274"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="43"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    <sequence length="320" mass="35806" checksum="2EED75DF7AFCB975" modified="2023-09-13" version="1">MEIVSIRNQTITEFVLLGFSDVAELHLLFFIVFTLIYTSIIIGNMLIIVAVVSSPRLHTPMYFFLVNLSFLEILYTSTVVPKMLEGFLQEAAISVAGCLLQFFIFGSLATAECVLLAVMAYDRYLAICHPLHYPLLMRPRWCLGLVVIAWLSGFMLDGLAVALIAQLRFCGPNHIDHFYCDFMPLMGLACSDPSIAQMATFILSVVCLTVPFGLILTSYARIVVAVLRVPAGASRRKAFSTCSSHLAVVSTFYGTLIALYIAPSAVHSQIFSKVFALLYTVFTPLFNPVIYTLRNKEVHQALRRILYISKRKHLIEGGWW</sequence>
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                <person name="Guo"/>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <text>a primary alcohol + NAD(+) = an aldehyde + NADH + H(+)</text>
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    <comment type="subcellular location">
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    <comment type="similarity">
        <text evidence="11">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
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        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
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    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
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    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
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    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR43880">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880:SF1">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 1A"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ADH_ZINC"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="12">
        <location>
            <begin position="73"/>
            <end position="422"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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            <dbReference type="ARBA" id="ARBA00013190"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    <evidence type="ECO:0000256" key="5">
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            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049243"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00829"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005053006.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="424" mass="45026" checksum="E9BB550DCC900CD5" modified="2023-09-13" version="1">MGVASRPQMTRVELGITGANKTSVQSICLHPQEGIPQGFVRLLACRENGIMSTAGKVIKCKAAVLWEQKKPFSIEEVEVAPPKAHEVRIKMVAAGICRSDDHVVSGTLVAPLPVIAGHEAAGIVESIGEGVTTVRPGDKVIPLFTPQCGKCSVCKHPEGNLCLKNLSTPRGTMQDGTSRFTCRGKPIHHFLGISTFSQYTVVDEISVAKIDAASPLEKVCLIGCGFSTGYGSAVKVAKVTQGSTCAVFGLGGVGLSVIMGCKAAGAARIIGVDINKDKFEKAKEVGATECINPQDFEKPIQEVLTEMSNGGVDFSFEVIGRLETMVAALSCCQEAYGVSVIVGVPPDSQNLSMNPMLLLSGRTWKGAIFGGFKSKDSVPKLVADFMAKKFALDPLITHVLPFEKINEGFDLLRSGKSIRTILTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JIM8</accession>
    <name>A0A9L0JIM8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">AP2-associated protein kinase 1</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Adaptor-associated kinase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">AAK1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Preferentially, may phosphorylate substrates on threonine residues. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <location evidence="1">Clathrin-coated pit</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Presynapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0JIM8"/>
    <dbReference type="Ensembl" id="ENSEAST00005075883.1">
        <property type="protein sequence ID" value="ENSEASP00005049045.1"/>
        <property type="gene ID" value="ENSEASG00005007366.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155968"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14037">
        <property type="entry name" value="STKc_NAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000072">
        <property type="entry name" value="AP2 associated kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051744">
        <property type="entry name" value="AP2_assoc_SerThr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47907:SF5">
        <property type="entry name" value="AP2 ASSOCIATED KINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47907">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="46"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="327"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="576"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="665"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="837"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="923"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="436"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="449"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="488"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="511"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="529"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="617"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="673"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="845"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="932"/>
            <end position="945"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00054570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005049045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="962" mass="104241" checksum="7A6711EBD44E1B3D" modified="2023-09-13" version="1">MKKFFDSRREQGCSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINNVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAVARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKIITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECPIPNVQNSPIPAKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPAPQQPPSTPAQGLPTQAQATPQHQQQLFLKQQQQQQQQQQQPPPPSQQPAGTFYQQQQQAQAQQFQAVHPATQQPAIAQFPVVSQGSSQQQLMQNFYQQQQQQQQQQQLATTLHQQQLLTQQAALQQKTAVAAVQQPQAQQTTAPQPPPAQEPAIQAPVRQQPKVQTTPPPAIQGQKLGSLTPPSSKTQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRTSQQNVYNPSESSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKHPEKLGGSAESLIPGFQPTQGDAFATASFPAGAAEKRKGGQTVDPSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDYSLLSNPTADLLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGGHSRNSSGSSESSLPNLARSLLLVDQLIDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JK30</accession>
    <name>A0A9L0JK30_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Zinc finger and BTB domain containing 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ZBTB9</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014701790.1">
        <property type="nucleotide sequence ID" value="XM_014846304.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JK30"/>
    <dbReference type="Ensembl" id="ENSEAST00005050914.1">
        <property type="protein sequence ID" value="ENSEASP00005053564.1"/>
        <property type="gene ID" value="ENSEASG00005036178.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005065671.1">
        <property type="protein sequence ID" value="ENSEASP00005054988.1"/>
        <property type="gene ID" value="ENSEASG00005036178.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106834588"/>
    <dbReference type="KEGG" id="eai:106834588"/>
    <dbReference type="CTD" id="221504"/>
    <dbReference type="GeneTree" id="ENSGT00940000162408"/>
    <dbReference type="OMA" id="PGGWCIR"/>
    <dbReference type="OrthoDB" id="6601382at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000145">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF13">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="9">
        <location>
            <begin position="48"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="399"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="207"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="321"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="216"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005053564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="461" mass="49076" checksum="22BEA7D041D88017" modified="2023-09-13" version="1">MDTSTPLPPVAPSPNFSPAPRTIQIEFPQHSSLLLEALNRHRLEGKFCDVSLLVQGRELRAHKAVLAAASPYFHDKLLLRDAPRLTLPSVIEADAFEGLLQLIYSGRLRLPLDALPAHLLVASGLQMWQVVDQCSEILRDLETSGGGLSTPGSTACHTLLSTTSSPGGWCISSSSFQTEVQSSTSTQSLVGGEGSELEDVLQIQIEEEEEEDQGSAAPSQTPQSQRASGDFPRPHGSQPLPVSSTPRRLPEGESAPLEPPAPQTARPLKVFYIKQEPFEPKEEKSGGGTQSGAAEEETKVFAGGGSEGNGELAFLLPSAAGAASGGAGSSGKPVDLHGNEIVSGSGGPGGAGQAVHGPVKLGGAPPADGKRFGCLCGKRFAVKPKRDRHIMLTFSLRPFGCGICNKRFKLKHHLTEHMKTHAGALHACPHCGRQFRVHAYFLRHQDLCKGQGWATAHWTYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JU42</accession>
    <name>A0A9L0JU42_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA polymerase II-associated factor 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PAF1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PAF1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014715713.1">
        <property type="nucleotide sequence ID" value="XM_014860227.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JU42"/>
    <dbReference type="Ensembl" id="ENSEAST00005073375.1">
        <property type="protein sequence ID" value="ENSEASP00005053532.1"/>
        <property type="gene ID" value="ENSEASG00005012805.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106843276"/>
    <dbReference type="KEGG" id="eai:106843276"/>
    <dbReference type="CTD" id="54623"/>
    <dbReference type="GeneTree" id="ENSGT00940000166250"/>
    <dbReference type="OMA" id="LVCRIKY"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007133">
        <property type="entry name" value="RNA_pol_II-assoc_Paf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23188">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23188:SF12">
        <property type="entry name" value="RNA POLYMERASE II-ASSOCIATED FACTOR 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03985">
        <property type="entry name" value="Paf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="361"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="361"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="375"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="389"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="442"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="458"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="506"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005053532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="524" mass="59384" checksum="1CF0F5C8CDF04D76" modified="2023-09-13" version="1">MAPTIQTQAQREDGHRPNSHRTLPERSGVVCRVKYCNSLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVLLDPADEKLLEEEIQAPTSSKRSQQHAKVVPWMRKTEYISTEFNRYGISNEKPEVKIGVSVKQQFTEEEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMDYAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRAKAGVQSGTNALLVVKHRDMNEKELEAQEARKAQLENHEPEEEEEEEMETEEKEAGGSDDEREKDSSSEKEGSEDERSGSESEREEGDRDEASDKSGSGEEESSEDEARAARDKEEIFGSDADSEDDADSDEEDRGRARGSDDSDSGSDGGGQRSRSASPFPSGSEHSAQDGSEAAASDSSEADSDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JVW8</accession>
    <name>A0A9L0JVW8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Thyrotropin-releasing hormone-degrading ectoenzyme</fullName>
            <ecNumber evidence="18">3.4.19.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Pyroglutamyl-peptidase II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">TRH-specific aminopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Thyroliberinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">TRHDE</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Specific inactivation of TRH after its release.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Release of the N-terminal pyroglutamyl group from pGlu-|-His-Xaa tripeptides and pGlu-|-His-Xaa-Gly tetrapeptides.</text>
            <dbReference type="EC" id="3.4.19.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="24">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="24">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.6" evidence="18"/>
    <dbReference type="RefSeq" id="XP_014682596.1">
        <property type="nucleotide sequence ID" value="XM_014827110.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JVW8"/>
    <dbReference type="Ensembl" id="ENSEAST00005081800.1">
        <property type="protein sequence ID" value="ENSEASP00005056603.1"/>
        <property type="gene ID" value="ENSEASG00005021964.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822089"/>
    <dbReference type="KEGG" id="eai:106822089"/>
    <dbReference type="CTD" id="29953"/>
    <dbReference type="GeneTree" id="ENSGT00940000155878"/>
    <dbReference type="OMA" id="HKRLVMG"/>
    <dbReference type="OrthoDB" id="6750768at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016920">
        <property type="term" value="F:pyroglutamyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000006">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000005">
        <property type="entry name" value="Aminopeptidase N-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000008">
        <property type="entry name" value="Thyrotropin-releasing hormone-degrading ectoenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000007">
        <property type="entry name" value="thyrotropin-releasing hormone-degrading ectoenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF294">
        <property type="entry name" value="THYROTROPIN-RELEASING HORMONE-DEGRADING ECTOENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9 24" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="29">
        <location>
            <begin position="188"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="27">
        <location>
            <begin position="410"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="28">
        <location>
            <begin position="720"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="118"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="26">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="23">
        <location>
            <position position="483"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="482"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="486"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="505"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="25">
        <location>
            <position position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00055732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00066574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00072445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00076496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00079759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00083577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005056603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1066" mass="121653" checksum="FF203CB8253B1DAD" modified="2023-09-13" version="1">MALDGERGEQEEEKKKKKKKKKRKKKKEEEEGAEKSSSPFAATMGENDAALRAGGRGLSDPWADSVGVRPCTTERHITVHKRLVLAFAVSIVALLAVTMLAVLLSLRFDECGARAAPGADGGPTGFPARGGNESLPGSARRHPHAGGDPGQPEAGEAAPGTPSAQPPSEEEREQWQPWTQLRLSGYLKPLHYNLMLTAFMENFTFSGEVNVEIACRNATRYVVLHASRVAVEKVQVAEDRVAGAVPVAGFFLYPQTQVLVVVLNRTLDAQRNYNLKIIYNALIENELLGFFRSSYVLHGERRFLGVTQFSPTHARKAFPCFDEPIYKATFKISIKHQATYLSLSNMPVETSVFEEDGWVTDHFSQTPLMSTYYLAWAICNFTYRETTTKSGVVVRLYARPDAIRRGSGDYALHITKRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSIFVEQRILLDPSVSSISYLLDVTMVIIHEICHQWFGDLVTPVWWEDVWLKEGFAHYFEFVGTDYLYPGWNMEKQRFLTDVLHEVMLLDGLASSHPVSQEVRQAADIDRVFDWIAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYGNAARNDLWNALSEALKRNGKYVNIQEVMDQWTLQMGYPVITILGNTTAENRIIITQQHFIYDISAKTKALELQNNSYLWQIPLTIVVGNRSHVSSEAIIWVSNKSEHHRITSLDKGSWLLGNINQTGYFRVNYDLRNWRLLIDQLIRNHEILSVSNRAGLIDDAFSLARAGYLPQNIPLEVIRYLSEEKDFLPWHAASRALYPLDKLLDRMEKYNVFNEYILKQVATTYIKLGWPKNNFNGSLVQASYQHEELRREVIMLACSFGNKHCHQQASTLISDWISSNRNRIPLNVRDIVYCTGVSLLDEDVWEFIWMKFHSTTAVSEKKILLEALTCSDDRNLLNRLLNLSLNSEVVLDQDAIDVIIHVARNPHGRDLAWKFFRDKWKILNTRYGEALFMNSKLISGVTEFLNTEGELKELKTFMKSYDGVAAASFSRAVETVEANVRWKMLYQDELFQWLGKALRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JXG3</accession>
    <name>A0A9L0JXG3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14">Multifunctional fusion protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="13">Interleukin-1</fullName>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="14">Interleukin-1 beta</fullName>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="14" type="primary">IL1B</name>
        <name evidence="16" type="synonym">LOC106838557</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12 14">Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Monomer. In its precursor form, weakly interacts with full-length MEFV; the mature cytokine does not interact at all. Interacts with integrins ITGAV:ITGBV and ITGA5:ITGB1; integrin-binding is required for IL1B signaling. Interacts with cargo receptor TMED10; the interaction is direct and is required for the secretion of IL1B mature form. Interacts with HSP90AB1; the interaction facilitates cargo translocation into the ERGIC. Interacts with HSP90B1; the interaction facilitates cargo translocation into the ERGIC.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Monomer. Interacts with MEFV.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cytoplasm</location>
            <location evidence="14">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Lysosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 14">Secreted</location>
            <location evidence="2 14">Extracellular exosome</location>
        </subcellularLocation>
        <text evidence="14">The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. Mature form is secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. In contrast, the precursor form is not released, due to the presence of an acidic region that is proteolytically removed by CASP1 during maturation. The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="14">IL1B production occurs in 2 steps, each being controlled by different stimuli. First, inflammatory signals, such as LPS, stimulate the synthesis and promote the accumulation of cytosolic stores of pro-IL1B (priming). Then additional signals are required for inflammasome assembly, leading to CASP1 activation, pro-IL1B processing and eventually secretion of the active cytokine. IL1B processing and secretion are temporarily associated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the IL-1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014708229.1">
        <property type="nucleotide sequence ID" value="XM_014852743.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JXG3"/>
    <dbReference type="Ensembl" id="ENSEAST00005082327.1">
        <property type="protein sequence ID" value="ENSEASP00005057971.1"/>
        <property type="gene ID" value="ENSEASG00005030429.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106838557"/>
    <dbReference type="KEGG" id="eai:106838557"/>
    <dbReference type="GeneTree" id="ENSGT00950000182943"/>
    <dbReference type="OrthoDB" id="9449069at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005149">
        <property type="term" value="F:interleukin-1 receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019221">
        <property type="term" value="P:cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001660">
        <property type="term" value="P:fever generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051781">
        <property type="term" value="P:positive regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033092">
        <property type="term" value="P:positive regulation of immature T cell proliferation in thymus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23296">
        <property type="entry name" value="beta-trefoil_IL1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000027">
        <property type="entry name" value="Interleukin-1 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020877">
        <property type="entry name" value="IL-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000975">
        <property type="entry name" value="IL-1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003502">
        <property type="entry name" value="IL-1_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008996">
        <property type="entry name" value="IL1/FGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10078:SF30">
        <property type="entry name" value="INTERLEUKIN-1 BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10078">
        <property type="entry name" value="INTERLEUKIN-1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00340">
        <property type="entry name" value="IL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02394">
        <property type="entry name" value="IL1_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00262">
        <property type="entry name" value="IL1HBGF"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00264">
        <property type="entry name" value="INTERLEUKIN1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01359">
        <property type="entry name" value="INTRLEUKIN1B"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01357">
        <property type="entry name" value="INTRLEUKN1AB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00125">
        <property type="entry name" value="IL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50353">
        <property type="entry name" value="Cytokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00253">
        <property type="entry name" value="INTERLEUKIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0202">Cytokine</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 13" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="9 14" id="KW-0458">Lysosome</keyword>
    <keyword evidence="10 13" id="KW-0497">Mitogen</keyword>
    <keyword evidence="7 13" id="KW-0666">Pyrogen</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Interleukin-1 propeptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU364119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005057971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="268" mass="30310" checksum="B052449A5C7EA70E" modified="2023-09-13" version="1">MAAVPDTSDMMTYCSGNENDLFFEEDGPKQMKGSFQDLDLSSTGDGGIQLQFSHQLYNKTFKHVVSIIVAMEKLKKIPVPCSQAFQDDDLRSLFSVIFEEEPIICDNWDDDYVCDAAVHSVNCRLRDIYHKSLVLSGACELQAVHLNGENTNQQVVFCMSFVQGEEETDKIPVALGLKEKNLYLSCGMKDGKPTLQLETVDPNTYPKRKMEKRFVFNKMEIKGNVEFESAMYPNWYISTSQAEKKPVFLGNTRGGRDITDFIMEITSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JYK3</accession>
    <name>A0A9L0JYK3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">V-type proton ATPase subunit C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ATP6V1C1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 10">Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex. The V1 complex consists of three catalytic AB heterodimers that form a heterohexamer, three peripheral stalks each consisting of EG heterodimers, one central rotor including subunits D and F, and the regulatory subunits C and H. The proton translocation complex V0 consists of the proton transport subunit a, a ring of proteolipid subunits c9c'', rotary subunit d, subunits e and f, and the accessory subunits ATP6AP1/Ac45 and ATP6AP2/PRR.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">V-ATPase is a heteromultimeric enzyme made up of two complexes: the ATP-hydrolytic V1 complex and the proton translocation V0 complex. The V1 complex consists of three catalytic AB heterodimers that form a heterohexamer, three peripheral stalks each consisting of EG heterodimers, one central rotor including subunits D and F, and the regulatory subunits C and H. The proton translocation complex V0 consists of the proton transport subunit a, a ring of proteolipid subunits c9c'', rotary subunit d, subunits e and f, and two accessory subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasmic vesicle</location>
            <location evidence="6">Clathrin-coated vesicle membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasmic vesicle</location>
            <location evidence="7">Secretory vesicle</location>
            <location evidence="7">Synaptic vesicle membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the V-ATPase C subunit family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014827689.3"/>
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        <property type="nucleotide sequence ID" value="XM_014827771.3"/>
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        <property type="nucleotide sequence ID" value="XM_070481433.1"/>
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        <property type="gene ID" value="ENSEASG00005001297.2"/>
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    <dbReference type="GeneTree" id="ENSGT00390000004263"/>
    <dbReference type="OMA" id="VMIWIHV"/>
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        <property type="component" value="Chromosome 12"/>
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        <property type="term" value="C:clathrin-coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000221">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V1 domain"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:synaptic vesicle lumen acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14785">
        <property type="entry name" value="V-ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="V-type proton ATPase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000002">
        <property type="entry name" value="V-type proton ATPase subunit C"/>
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    </dbReference>
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        <property type="entry name" value="V-type proton ATPase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Vacuolar atp synthase subunit c, domain 1"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR004907">
        <property type="entry name" value="ATPase_V1-cplx_csu"/>
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        <property type="entry name" value="Vac_ATP_synth_c_sf"/>
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        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT C 1"/>
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        <property type="entry name" value="Vacuolar ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="5 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005054190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="382" mass="44000" checksum="E93B80D11B60CB29" modified="2023-09-13" version="1">MTEFWLISAPGEKTCQQTWEKLHAATTKNNNLAISSKFNIPDLKVGTLDVLVGLSDELAKLDAFVEGVVKKVAQYMADVLEDSKDKVQENLLANGVDLVTYITRFQWDMAKYPIKQSLKNISEIIAKGVTQIDNDLKSRASAYNNLKGNLQNLERKNAGSLLTRSLAEIVKKDDFVLDSEYLITLLVVVPKLNHNDWIKQYETLADMVVPRSSNVLSEDQDSYLCNVTLFRKAVDDFRHKARENKFIVRDFQYNEEEMKADKEEMNRLSTDKKKQFGPLVRWLKVNFSEAFIAWIHVKALRVFVESVLRYGLPVNFQAMLLQPNKKTMKKLREVLYELYKHLDSSAAAIIDAPMDIPGLNLSQQEYYPYVYYKIDCNLLEFK</sequence>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="Guo"/>
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                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Fang"/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
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                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_044613284.1">
        <property type="nucleotide sequence ID" value="XM_044757349.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JZ27"/>
    <dbReference type="Ensembl" id="ENSEAST00005069402.1">
        <property type="protein sequence ID" value="ENSEASP00005058389.1"/>
        <property type="gene ID" value="ENSEASG00005037754.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 24"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903580">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000183">
        <property type="term" value="P:rDNA heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018379">
        <property type="entry name" value="BEN_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033583">
        <property type="entry name" value="BEND3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28665">
        <property type="entry name" value="BEN DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28665:SF1">
        <property type="entry name" value="BEN DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10523">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM01025">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51457">
        <property type="entry name" value="BEN"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BEN" evidence="2">
        <location>
            <begin position="242"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="BEN" evidence="2">
        <location>
            <begin position="387"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="BEN" evidence="2">
        <location>
            <begin position="548"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="domain" description="BEN" evidence="2">
        <location>
            <begin position="715"/>
            <end position="816"/>
        </location>
    </feature>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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        </source>
    </evidence>
    <sequence length="828" mass="94364" checksum="D1A200F8529B6CF8" modified="2023-09-13" version="1">MNATEFSEDVEEVLKNNTVKVETEAEDAALDCSVNSRSAEKHPLDSVFTALQDSSKRKQPGSDGQPDSVPSVKRRRLIPEALLAGMRNRENSSPCQGNGEQAGRGKNSGSVWPGEEEPCNDVTTPSYKKPLYGISHKIMEKKNPPMGDALNTYELFEKANAGNSPSPLRLLNEPQKRDCGSATVATDGDPNIYFLIQKMFYMLNTLSSNMSQLHSKVDLLSLEVSRIKKQVSPTEMVAKFQPPPEYQLTAAELKQIVDQSLSGGDLACRLLVQLFPELFSDVDFSRGCSACGFAAKRKLESLHLQLIRNYVEVYYPSVKDTAVWQAECLPQLNDFFSRFWAQREMEDSQPGGQVASFFEPEQVDAGHFLDNKDQEEALSLDRSSTIASDHVVDTQDLTELLDEASSPGEFAVFLLHRLFPELFDHRKLGEQYSCYGDGGKQELDPQRLQIIRNYTEIYFPDMQEEEAWLQQCAQRINDELEGLGLDAGSEGEPPRDDCYDSSSLPDDISVVKVEDSFEGERPGRRSKKIWLVPIDFEKLEIPQPDFEVPGADCLLSKEQLRSIYESSLSIGNFASRLLVHLFPELFTHENLRKQYNCSGSLGKKQLDPSRIKLIRHYVQLLYPRAKNDRVWTLEFVGKLDERCRRRDTEQRRSYQQQRKVHVPGPECRDLASYAINPERFREEFEGPPLPPERSSKDFCKIPLDELVVPSPDFPVPSLYLLSDKEVREIVQQSLSVGNFAARLLVRLFPELFTAENLRLQYNHSGACNKKQLDPTRLRLIRHYVEAVYPVEKMEEVWHYECIPSIDERCRRPNRKKCDILKKAKKVEK</sequence>
</entry>
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        <name type="scientific">Equus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="X."/>
                <person name="Zhao"/>
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                <person name="Wang"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Li"/>
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                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
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                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
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                <person name="Jin"/>
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                <person name="Yue"/>
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                <person name="Liu"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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            <authorList>
                <consortium name="Ensembl"/>
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            <end position="49"/>
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    <feature type="transmembrane region" description="Helical" evidence="1">
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            <dbReference type="SAM" id="Phobius"/>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
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    <sequence length="101" mass="11484" checksum="59A84278EF795FB3" modified="2023-09-13" version="1">MISDDEHLFVCLLATLISSLEKCLFMSSAHFLSRLFVLLLLSCVSSLYIMEINPLSDKWLVNIFSQLMGCFFVSILFSLALKKLFSLMKSHLFILSIVSLN</sequence>
</entry>
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    <accession>A0A9L0K0G9</accession>
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            <taxon>Equidae</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="X. and Zhong"/>
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            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="component" value="Chromosome 12"/>
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        <property type="entry name" value="Protein tyrosine phosphatase type IVA 3"/>
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    </dbReference>
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        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
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    <dbReference type="PANTHER" id="PTHR23339">
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    </dbReference>
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            <end position="110"/>
        </location>
    </feature>
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        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005055827.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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            <fullName evidence="29">ornithine decarboxylase paralog</fullName>
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        <name type="scientific">Equus asinus</name>
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    <reference evidence="33 34" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="G."/>
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                <person name="J."/>
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                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="25">Antizyme inhibitor (AZI) protein that positively regulates ornithine decarboxylase (ODC) activity and polyamine uptake. AZI is an enzymatically inactive ODC homolog that counteracts the negative effect of ODC antizymes (AZs) OAZ1, OAZ2 and OAZ3 on ODC activity by competing with ODC for antizyme-binding. Inhibits antizyme-dependent ODC degradation and releases ODC monomers from their inactive complex with antizymes, leading to formation of the catalytically active ODC homodimer and restoring polyamine production. Participates in the morphological integrity of the trans-Golgi network (TGN) and functions as a regulator of intracellular secretory vesicle trafficking.</text>
    </comment>
    <comment type="function">
        <text evidence="24">Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. Polyamines are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication to apoptosis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="27">Monomer. Interacts with OAZ1, OAZ2 and OAZ3; this interaction disrupts the interaction between the antizyme and ODC1. Does not form a heterodimer with ODC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell projection</location>
            <location evidence="9">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasmic granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Endoplasmic reticulum-Golgi intermediate compartment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">cis-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="26">Belongs to the Orn/Lys/Arg decarboxylase class-II family. ODC antizyme inhibitor subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K552"/>
    <dbReference type="Ensembl" id="ENSEAST00005060638.1">
        <property type="protein sequence ID" value="ENSEASP00005060479.1"/>
        <property type="gene ID" value="ENSEASG00005011775.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182995"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990005">
        <property type="term" value="C:granular vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042978">
        <property type="term" value="F:ornithine decarboxylase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042177">
        <property type="term" value="P:negative regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006591">
        <property type="term" value="P:ornithine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902269">
        <property type="term" value="P:positive regulation of polyamine transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033387">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098629">
        <property type="term" value="P:trans-Golgi network membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00622">
        <property type="entry name" value="PLPDE_III_ODC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.37.10:FF:000009">
        <property type="entry name" value="antizyme inhibitor 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000005">
        <property type="entry name" value="Ornithine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="entry name" value="Lyase, Ornithine Decarboxylase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022657">
        <property type="entry name" value="De-COase2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002433">
        <property type="entry name" value="Orn_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482:SF4">
        <property type="entry name" value="ANTIZYME INHIBITOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11482">
        <property type="entry name" value="ARGININE/DIAMINOPIMELATE/ORNITHINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01182">
        <property type="entry name" value="ORNDCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="Alanine racemase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00879">
        <property type="entry name" value="ODR_DC_2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="22" id="KW-0966">Cell projection</keyword>
    <keyword evidence="14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="23" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="17" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="20" id="KW-0456">Lyase</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="16" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0813">Transport</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Orn/DAP/Arg decarboxylase 2 N-terminal" evidence="32">
        <location>
            <begin position="156"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="Orn/DAP/Arg decarboxylase 2 N-terminal" evidence="32">
        <location>
            <begin position="234"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00037173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00058216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00061098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00062007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00071561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00081150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00081984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005060479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="600" mass="65424" checksum="85F7DE7C10D68210" modified="2023-09-13" version="1">MRFKASAGSGSGSLGERAPRAPMLTGPRPLLPPHSRGLGSATATPPRGVAQVSRSPATPPSSTPVSGPREGGRGLCVAYFLRRQRRRQQQQQQQQQLRLHPAHRLLLLGMAGYLSETDFVMVEEGFSTRDLLEELTLGASQATTDEVAAFFVADLGAVVRKHFCFLKCLPRVRPFYAVKCNSSLGVLKVLAELGLGFSCANKHPLLKPLLPDLHPHCSKESPLTNVPSDRHDPAEMELVQRIGVPASKIIYANPCKQIAHIKYAAKHGVRLLSFDNEIELAKVVKSHPSARMVLCIATDDSHSLSRLSFKFGASLKSCRHLLENAKKSHVEVVGVSFHIGSDCPDPQAYAQSIADARLVFEIGAELGHRMHILDLGGGFPGVEGTKVRFEEITSVINSALDLYFPEDCGVDILAKLGRYYVTSAFTLAISIIAKREVLLDQPSREEENSSAPKTIVYHLHEGVYGIFNSVLFDNTCPTPILQKKPSTEQPLYSSSLWGPAVDGCDCVAEGLWLPQLHVGDWLVFEHMGAYTVGMGSRLGETQTCRVTYAMSRLAWEALRGQLLPAEQDEDAEGVCKPLSCGWEITDTLCVGPVFTPASIM</sequence>
</entry>
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    <accession>A0A9L0K8G2</accession>
    <name>A0A9L0K8G2_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ELKS/RAB6-interacting/CAST family member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ERC1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Presynapse</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K8G2"/>
    <dbReference type="Ensembl" id="ENSEAST00005078148.1">
        <property type="protein sequence ID" value="ENSEASP00005061639.1"/>
        <property type="gene ID" value="ENSEASG00005018526.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00650000093320"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <keyword evidence="6 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
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            <end position="1112"/>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K8P7</accession>
    <name>A0A9L0K8P7_EQUAS</name>
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        <submittedName>
            <fullName evidence="8">Ninjurin 2</fullName>
        </submittedName>
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    <gene>
        <name evidence="8" type="primary">NINJ2</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Zhang"/>
                <person name="R."/>
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                <person name="J."/>
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                <person name="X."/>
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                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
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            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="2">Belongs to the ninjurin family.</text>
    </comment>
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        <property type="gene ID" value="ENSEASG00005018495.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 22"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:tissue regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007007">
        <property type="entry name" value="Ninjurin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12316:SF24">
        <property type="entry name" value="NINJURIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NINJURIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04923">
        <property type="entry name" value="Ninjurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="153"/>
            <end position="173"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="188" mass="20467" checksum="508886A2FBEB78E1" modified="2023-09-13" version="1">IVGLSCQLCALSHPKKAAETQTGKPGIAHAVCYRHPVSVKALESSKMESEREIINLQPGSPDSRSQPVNLNHYATKKSVAESMLDAALFMTNATRLKAVLEQGPSSNYYSTLVTLISISLLLQVVIGILLVVIARLNLNEVEKQWRLNQLNNAATTLIFITVVINVFITAFGAHKTGFLASRTSRNPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K9F6</accession>
    <name>A0A9L0K9F6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">USP6 N-terminal-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC123277938</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="S."/>
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                <person name="Y."/>
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                <person name="Q."/>
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                <person name="Q."/>
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                <person name="X."/>
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                <person name="Y."/>
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                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Acts as a GTPase-activating protein for RAB5A and RAB43. Involved in receptor trafficking. In complex with EPS8 inhibits internalization of EGFR. Involved in retrograde transport from the endocytic pathway to the Golgi apparatus. Involved in the transport of Shiga toxin from early and recycling endosomes to the trans-Golgi network. Required for structural integrity of the Golgi complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with EPS8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K9F6"/>
    <dbReference type="Ensembl" id="ENSEAST00005044320.1">
        <property type="protein sequence ID" value="ENSEASP00005059027.1"/>
        <property type="gene ID" value="ENSEASG00005036214.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161238"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000010">
        <property type="entry name" value="Putative USP6 N-terminal-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.750:FF:000001">
        <property type="entry name" value="TBC1 domain family member 10A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000019">
        <property type="entry name" value="USP6 N-terminal like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF25">
        <property type="entry name" value="RAB-GAP TBC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="12">
        <location>
            <begin position="100"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="355"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="426"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="438"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005059027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="457" mass="52015" checksum="BE44D3E0E977CD64" modified="2023-09-13" version="1">MKEDPETLLALERAKIIAQYDQGRQAGVPVDPQEDPELPLFKVTDRLGFLHEKELPSLTTQEAKHKRRDIRRADKWWLKMLRDWGQYQGSKKMHKRVFKGIPPQVRGRVWSLLLDLEKVKEENKGKYERMKEQARLFSRDIKQIDLDVNRTFRNHIMYWDRYGIKQQALFHVLAAYSVYNTEVGYCQGMSEVAAVLLMFLGEEDAFWALAQLMVSERHAMHGFFVPGFPKLLRFQAHHEQVLTKGLPKLKQHLDEEHMCTGIYTAKWFLQCFIGRTPFSLTLKLWDTYILEGERVLTAMAYTVLKLHKKRLLKLPLEGLREFLQDGLCQPWALEDDAVLKHLRASMVELQRMKCDLPPPAKPEEFPTLPLGLERASPEPRPVLASPGCEKLPSEAGLTLPHLPVEHERPGPSPAQVVPEAEEPQNEGASTGPTAAHTSLGAPRGSGSGLPAPGPGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KBR5</accession>
    <name>A0A9L0KBR5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ATP synthase lipid-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">ATPase protein 9</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">ATPase subunit c</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ATP5MC1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the ATPase C chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014689393.1">
        <property type="nucleotide sequence ID" value="XM_014833907.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014689395.1">
        <property type="nucleotide sequence ID" value="XM_014833909.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0KBR5"/>
    <dbReference type="Ensembl" id="ENSEAST00005067007.1">
        <property type="protein sequence ID" value="ENSEASP00005062902.1"/>
        <property type="gene ID" value="ENSEASG00005032484.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106826529"/>
    <dbReference type="KEGG" id="eai:106826529"/>
    <dbReference type="CTD" id="516"/>
    <dbReference type="GeneTree" id="ENSGT00940000159720"/>
    <dbReference type="OrthoDB" id="438052at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033177">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, proton-transporting domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18182">
        <property type="entry name" value="ATP-synt_Fo_c_ATP5G3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.20.10:FF:000003">
        <property type="entry name" value="Atp synthase f complex subunit mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.20.10">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01396">
        <property type="entry name" value="ATP_synth_c_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000454">
        <property type="entry name" value="ATP_synth_F0_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020537">
        <property type="entry name" value="ATP_synth_F0_csu_DDCD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038662">
        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031:SF56">
        <property type="entry name" value="ATP SYNTHASE F(0) COMPLEX SUBUNIT C1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10031">
        <property type="entry name" value="ATP SYNTHASE LIPID-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00124">
        <property type="entry name" value="ATPASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00605">
        <property type="entry name" value="ATPASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0138">CF(0)</keyword>
    <keyword evidence="7 17" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="10 17" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 17" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="70"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="108"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="18">
        <location>
            <begin position="71"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005062902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="137" mass="14377" checksum="78C9A93E7BBA4D2E" modified="2023-09-13" version="1">MQTTGALLIPPALVRCCTRGLIRPVSASFLSRPEIPSNQQPSYSSSPLQVARREFQTSVVSRDIDTAAKFIGAGAATVGVAGSGAGIGTVFGSLIIGYARNPSLKQQLFSYAILGFALSEAMGLFCLMVAFLILFAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KCL4</accession>
    <name>A0A9L0KCL4_EQUAS</name>
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        <submittedName>
            <fullName evidence="3">Ubiquitin conjugating enzyme E2 V2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">UBE2V2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_014844861.3"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A9L0KCL4"/>
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        <property type="protein sequence ID" value="ENSEASP00005063130.1"/>
        <property type="gene ID" value="ENSEASG00005024827.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="eai:106833588"/>
    <dbReference type="CTD" id="7336"/>
    <dbReference type="GeneTree" id="ENSGT00740000115534"/>
    <dbReference type="OMA" id="NWSREST"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
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        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="C:UBC13-MMS2 complex"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000729">
        <property type="term" value="P:DNA double-strand break processing"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042275">
        <property type="term" value="P:error-free postreplication DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000781">
        <property type="term" value="P:positive regulation of double-strand break repair"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902523">
        <property type="term" value="P:positive regulation of protein K63-linked ubiquitination"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd23807">
        <property type="entry name" value="UEV_UBE2V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000012">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 variant 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="2">
        <location>
            <begin position="45"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005063130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="180" mass="20044" checksum="012B9405C9FA3BBB" modified="2023-09-13" version="1">MTRAGKPWGGRGDTLPEVTRDVAVRASGCVGLQEKMAVSTGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTNYENRIYSLKVECGPKYPEAPPSVRFVTKINMNGINNSSGMVDARSIPVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEGQTYNN</sequence>
</entry>
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    <accession>A0A9L0KE66</accession>
    <name>A0A9L0KE66_EQUAS</name>
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        <recommendedName>
            <fullName evidence="16 19">Protein disulfide-isomerase</fullName>
            <ecNumber evidence="6 19">5.3.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">P4HB</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="S."/>
                <person name="Zhang"/>
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                <person name="Q."/>
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                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>Catalyzes the rearrangement of -S-S- bonds in proteins.</text>
            <dbReference type="EC" id="5.3.4.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Melanosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the protein disulfide isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.4.1" evidence="6 19"/>
    <dbReference type="RefSeq" id="XP_014719859.1">
        <property type="nucleotide sequence ID" value="XM_014864373.3"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A9L0KE66"/>
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        <property type="gene ID" value="ENSEASG00005005814.2"/>
    </dbReference>
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    <dbReference type="KEGG" id="eai:106845915"/>
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    <dbReference type="OMA" id="FFGMKKD"/>
    <dbReference type="OrthoDB" id="72053at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02961">
        <property type="entry name" value="PDI_a_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02995">
        <property type="entry name" value="PDI_a_PDI_a'_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02982">
        <property type="entry name" value="PDI_b'_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02981">
        <property type="entry name" value="PDI_b_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000023">
        <property type="entry name" value="Protein disulfide-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000030">
        <property type="entry name" value="Protein disulfide-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000110">
        <property type="entry name" value="Protein disulfide-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000027">
        <property type="entry name" value="protein disulfide-isomerase A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005788">
        <property type="entry name" value="PDI_thioredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005792">
        <property type="entry name" value="Prot_disulphide_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01130">
        <property type="entry name" value="ER_PDI_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01126">
        <property type="entry name" value="pdi_dom"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929">
        <property type="entry name" value="PROTEIN DISULFIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18929:SF101">
        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
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            <location evidence="1">Cytosol</location>
        </subcellularLocation>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="P53-induced death domain protein 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
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    <dbReference type="InterPro" id="IPR000488">
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    <dbReference type="InterPro" id="IPR025307">
        <property type="entry name" value="FIIND_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021861">
        <property type="entry name" value="THO_THOC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13265:SF1">
        <property type="entry name" value="CASPASE RECRUITMENT DOMAIN-CONTAINING PROTEIN 8"/>
        <property type="match status" value="1"/>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="531" mass="59781" checksum="0F37C7DD6ABC3124" modified="2023-09-13" version="1">MSCGARVAGSSEDHSASSEEKDCPTPNLDTSKDSKSGEDSSSQSSSESESDNEEPKPEKTIIQKVVTEHSPGPRTVVFMKDGELGESAAEEASAQGEEWEDKESRTDHFNSEFNKVQPYCEHCNAESKHHEQVTPRLLSRGQFLLKVDTEGIYQCTETGLIFEVNRKVDIKYCVLSWSKYADLVVKPWAVSGPLFDVKCDPACLTSIQFPHSLCLGHHDANMTFKVLHVKSSGALLESTVDHSATHVKWHVSSLSPVGPVMQSEETVYHHGAVILYKAIDHNPSLSFRVYIATNNESFIKDISKSIKHSSKKFMKIDKPPVCQKLLQNGKKYRLISEPEAEITPEEIEFVDGSLLKLKSYIEVYLEQPVEFKLLLVEMDSEEIVWKAKLRECDWIQHDQNRNISKSSMSGNRRRKLSSSLPDEEAYDKRMKQVDTSDGVKARSLLTDAQLITLAGKLGKEWTEVAMASLALDTRDIDDIQEKKDVTIYNFKVLKKWQEKEQGNATAQNLYNCLKNVPVEVQDMLKGFLRGT</sequence>
</entry>
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    <accession>A0A8C4KUX9</accession>
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        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
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        <name evidence="14" type="primary">LOC106830187</name>
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        <name type="scientific">Equus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Zhao"/>
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                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="G."/>
                <person name="Liu"/>
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                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
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                <person name="W."/>
                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Ji"/>
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                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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    <comment type="subcellular location">
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Olfactory receptor"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="entry name" value="Olfact_rcpt"/>
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        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
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        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF24">
        <property type="entry name" value="OLFACTORY RECEPTOR FAMILY 52 SUBFAMILY AC MEMBER 1"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
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        <location>
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            <end position="42"/>
        </location>
    </feature>
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        <location>
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        </location>
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    </feature>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
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    <accession>A0A8C4KVZ6</accession>
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        </lineage>
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    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Interacts with NFKB1, RELA and RELB; in the nucleus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NF-kappa-B inhibitor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KVZ6"/>
    <dbReference type="Ensembl" id="ENSEAST00005001997.2">
        <property type="protein sequence ID" value="ENSEASP00005001795.2"/>
        <property type="gene ID" value="ENSEASG00005001439.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153695"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000091">
        <property type="entry name" value="NF-kappa-B inhibitor delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124">
        <property type="entry name" value="ANKYRIN REPEAT FAMILY A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24124:SF7">
        <property type="entry name" value="NF-KAPPA-B INHIBITOR DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="244"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="277"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="11">
        <location>
            <begin position="403"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00063832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001795.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="473" mass="50401" checksum="58C0572CC4B0F945" modified="2023-09-13" version="2">MPGDPPSPPCPPTSGLGDLEVSRSERSHCPSQTVKKLLEEQRRRQQQPDAGGVPGQFPPPLAQPLTPSVNEAEAGHTHFPAHQETVGSGLGSLAPPTGFPDWDPNTHAAYTDSPYSYPAAAAENFLTSDFYPPSDPGRPCPFPLGMEEPLDTRLYAEPSLPQAGSWRGSGLPSGPPQLPPVVTGPSLDAARAHMLALGPQQLLAQDEEGDTLLHLFAARGLRWAAYAAAEILQVYRHLDIREHKGKTPLLVAAAANQPLIVEDLLNLGAEPNAADHQGRSVLHVAATYGLPGVLSAVINSGVRVDLEARDFEGLTPLHTAILALNVAMHPPDLCPRVLSTQARDRLACVQMLLHMGADHTSQEIKSNKTVLHLAVQAANPTLVQLLLELPRGDLRAFVNMKAHGNTALHMAAALPPGPPQEAIVRRLLAAGADPTLRNLENEQPVHLLRPGPGPEGLRQLLKRSRVAPPGLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KWN6</accession>
    <name>A0A8C4KWN6_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">SH2 domain containing adaptor protein B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SHB</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_014716624.2">
        <property type="nucleotide sequence ID" value="XM_014861138.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KWN6"/>
    <dbReference type="Ensembl" id="ENSEAST00005000954.2">
        <property type="protein sequence ID" value="ENSEASP00005000821.1"/>
        <property type="gene ID" value="ENSEASG00005000727.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106843895"/>
    <dbReference type="KEGG" id="eai:106843895"/>
    <dbReference type="CTD" id="6461"/>
    <dbReference type="GeneTree" id="ENSGT00940000161591"/>
    <dbReference type="OMA" id="RIMTELQ"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036464">
        <property type="term" value="C:cytoplasmic ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001784">
        <property type="term" value="F:phosphotyrosine residue binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030159">
        <property type="term" value="F:signaling receptor complex adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042100">
        <property type="term" value="P:B cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048514">
        <property type="term" value="P:blood vessel morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071425">
        <property type="term" value="P:hematopoietic stem cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900194">
        <property type="term" value="P:negative regulation of oocyte maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045931">
        <property type="term" value="P:positive regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045624">
        <property type="term" value="P:positive regulation of T-helper cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd10389">
        <property type="entry name" value="SH2_SHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000021">
        <property type="entry name" value="Putative SH2 domain-containing adapter protein F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051846">
        <property type="entry name" value="SH2_domain_adapters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035045">
        <property type="entry name" value="SHB_SH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15127">
        <property type="entry name" value="HEAVYWEIGHT, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15127:SF31">
        <property type="entry name" value="SH2 DOMAIN-CONTAINING ADAPTER PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="SH2" evidence="6">
        <location>
            <begin position="409"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="142"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="225"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="142"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="249"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="306"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="318"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="508" mass="54864" checksum="3D3C4AB813FD3B04" modified="2022-01-19" version="1">MAKWLNKYFSLGNSKTKNPPQPPRPDYREQRRRGERPSQPPQAAPQAPAAASASCGPAAASCFSASSGSLPDESGSTSDLIRAYRAQKERDFEDPYNGPGSSLRKLRAMCRLDYCGGGGEPGGGQRAFSASSASGAAGCCCASSGAGAAASSSSSSGSPHLYRSSSERRPATPAEVRYISPKHRLIKVESAAGGAGDPPGGACSGGRTWSPTACGGKKLLNKCAASAAEESGAGKKDKVTIADDYSDPFDAKNDPKSKAGKGESAGYMEPYEAQRIMTEFQRQESVRSQHKSIQLYDTPYEPEGQSVDSDSESTVSPRLRESKLPQDDDRPADEYDQPWEWNRVTIPALAAQFNGNEKRQSSPSPSRDRRRQLRAPGGGFKPIKHGSPEFCGALGERVDPAVPLEKQIWYHGAISRGDAENLLRLCKECSYLVRNSQTSKHDYSLSLKSNQGFMHMKLTKTKEKYVLGQNSPPFDSVPEVIHYYTTRKLPIKGAEHLSLLYPVAVRTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KZU3</accession>
    <name>A0A8C4KZU3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">A-type potassium channel modulatory protein DPP6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Dipeptidyl aminopeptidase-like protein 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Dipeptidyl peptidase 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Dipeptidyl peptidase VI</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">DPP6</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">Homodimer (in vitro). Interacts with KCND2. Identified in a complex with KCND2 and KCNIP2. Forms an octameric complex composed of four DPP6 subunits bound to the KCND2 tetramer. Interacts with KCND3; this interaction modulates the channel gating kinetics namely channel activation and inactivation kinetics and rate of recovery from inactivation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S9B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KZU3"/>
    <dbReference type="Ensembl" id="ENSEAST00005002364.2">
        <property type="protein sequence ID" value="ENSEASP00005002143.2"/>
        <property type="gene ID" value="ENSEASG00005001682.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156280"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015459">
        <property type="term" value="F:potassium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.140.10.30:FF:000001">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000003">
        <property type="entry name" value="Dipeptidyl peptidase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.140.10.30">
        <property type="entry name" value="Dipeptidylpeptidase IV, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002469">
        <property type="entry name" value="Peptidase_S9B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050278">
        <property type="entry name" value="Serine_Prot_S9B/DPPIV"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731:SF20">
        <property type="entry name" value="DIPEPTIDYL AMINOPEPTIDASE-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11731">
        <property type="entry name" value="PROTEASE FAMILY S9B,C DIPEPTIDYL-PEPTIDASE IV-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00930">
        <property type="entry name" value="DPPIV_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Dipeptidylpeptidase IV N-terminal" evidence="18">
        <location>
            <begin position="192"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="17">
        <location>
            <begin position="640"/>
            <end position="845"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF00930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005002143.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="862" mass="97182" checksum="D554C52F22AA9B99" modified="2023-09-13" version="2">MASLYQRFTGKINTSRSFPAPPEASHLLGGQGPEEDGAGPKPLGAQGPAAAPRERGGGGAGGRPRFQYQARSDCDDEDELVGSNPPQRNWKGIAIALLVILVICSLIVTSVILLTPAEDNSLSQKKKVTVEDLFSEDFRIHNPEAKWISDKEFIYREQKGNVILRNVETNMSTVLIEGKKIESLRAIRYEISPDKEYALFSYNVEPIYQHSYTGYYVLSKIPHGDPQSLDPPEVSNAKLQYAGWGPKGQQLIFIFENNIYYCAHVGKQAIRVVSTGKEGVIYNGLSDWLYEEEILKTHIAHWWSPDGTRLAYATINDSRVPVMELPTYTGSIYPTVKPYHYPKAGCENPSISLHVIGLNGPTHDLEMMPPDDPRMREYYVTMVKWATSTKVAVNWLSRAQNVSILTLCDATTGVCTKKHEDESEAWLHRQNEEPVFSQDGRKFFFVRAIPQGGQGKFYHITVSSSQPNSSNDNIQSITSGDWDVTKILSYDEKRNKIYFLSTEDLPRRRQLYSASTVGNFNRQCLSCDLVDNCTYFSASFSHSMDFFLLKCEGPGVPTVTVHNTTDKKKIFDLETNEHVQKAVNDRQMPKVEYRKIEIDDYNLPVQILKPATFTDTAHYPLLLVVDGTPGSQSVAEKFEVTWETVMVSSHGAVVVKCDGRGSGFQGTKLLHEVRRRLGSLEEKDQMEAVRTMLKEQYIDKTRVAVFGKDYGGYLSTYILPAKGENQGQIFTCGSALSPITDFKLYASAFSERYLGLHGLDNRAYEMTKVAHRVSALEEQQFLIIHATADEKIHFQHTAELITQLIKGKANYSLQIYPDESHYFNSASLKQHLYRSIINFFVECFRIQDKLPTATAKEEEEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L0I4</accession>
    <name>A0A8C4L0I4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Sodium/potassium-transporting ATPase subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">ATP1A4</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Cell membrane</location>
            <topology evidence="1 20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L0I4"/>
    <dbReference type="Ensembl" id="ENSEAST00005004220.2">
        <property type="protein sequence ID" value="ENSEASP00005003841.2"/>
        <property type="gene ID" value="ENSEASG00005002539.2"/>
    </dbReference>
    <dbReference type="KEGG" id="eai:106835754"/>
    <dbReference type="GeneTree" id="ENSGT00940000162378"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120200">
        <property type="term" value="C:rod photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005890">
        <property type="term" value="C:sodium:potassium-exchanging ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097225">
        <property type="term" value="C:sperm midpiece"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005391">
        <property type="term" value="F:P-type sodium:potassium-exchanging transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051649">
        <property type="term" value="P:establishment of localization in cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009566">
        <property type="term" value="P:fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030641">
        <property type="term" value="P:regulation of cellular pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd02608">
        <property type="entry name" value="P-type_ATPase_Na-K_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000106">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000001">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000004">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000095">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050510">
        <property type="entry name" value="Cation_transp_ATPase_P-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005775">
        <property type="entry name" value="P-type_ATPase_IIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01106">
        <property type="entry name" value="ATPase-IIC_X-K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294:SF3">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00121">
        <property type="entry name" value="NAKATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 20" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 20" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0915">Sodium</keyword>
    <keyword evidence="19" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7" id="KW-0740">Sodium/potassium transport</keyword>
    <keyword evidence="14" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 20" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="306"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="336"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="789"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="817"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="858"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="926"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="966"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="998"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="22">
        <location>
            <begin position="57"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022607"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
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            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
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            <dbReference type="Ensembl" id="ENSEASP00005003841.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1036" mass="115367" checksum="C084C3C82E001E0C" modified="2023-09-13" version="2">MEFGKTRGRVTPQELNPSSGPKPKRVPKMVGRRKKEKVKRKKNKANIEELKKEVVMDDHKLTLEELSRRYSVDLTKGLRPEKAQEILDRDGPNTLTPPPTTPEWVKFCKQLFGGFSILLWTGAILCFVAYGIQTYFNEEPTKDNLYLGIVLAVVVFITGCFSYYQEAKSSKIMESFRSMVPQQALVIRGGEKMQINVQNVVVGDLVEVKGGDRIPADIRLISAQGCKVDNSSLTGESEPQSRFPHFTHENLLETRNICFFSTNCVEGTARGIVIATGDSTVMGRIASLTSGLAVGQTPIATEIKHFIHIITMVAVFLGVSFFGLSLMLGYSWLKAVIFLIGIIVANVPEGLLVTVTVCLTLTAKRMAKKNCLVKNLEAVETLGSTSIICSDKTGTLTQNRMTVAHLWFDKTTYEANTSEEQTVNTFAKDSDTWFILARIAGLCNRADFKPDQETLPIAKRATTGDASESALLKFIEQSYSSVKEMREKSPKVAEIPFNSTNKYQISIHLREDSSQTHVLLMKGAPERILAFCSSYLLNGQEYSMDEEMKEAFQNAYLELGGLGERVLGFCFLNLPNTFSKGFKFNTDEVNFPMENFCFVGLISMIDPPRPAVPEAVCKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGTETAEDTTAQLKVHVRQIDSRDVKAIVVHGSDLKDMSSVQLDEILRNYTEIVFARTSPQQKLIIVEGCQRQGNVVAATGDGVNDSPALKKADIGIAMGVTGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLLFIILGIPLPLGTITILCIDLGTDMVPAISLAYESAESDIMKRAPRDPKNDKLVNYRLIGMAYGQIGMIQALAGFFTYFVILAENGFKPADLLGIRHDWENSYVNDLEDSYGQQWTFEQRKILEFTCQTGFFVSIVIVQWADLIICKTRRNSVFQQGMKNKILIFGILEETFLAAFLCYTPGMDMALRMYPLKIFWWLCAIPYSILIFIYDEVRKFAIRNHPGGWLERETYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L101</accession>
    <name>A0A8C4L101_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Transmembrane protease serine 2</fullName>
            <ecNumber evidence="18">3.4.21.122</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">TMPRSS2</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>The enzyme cleaves angiotensin-converting enzyme 2 (EC 3.4.17.23) and cleaves influenzea A and B virus and coronavirus spike glycoproteins at arginine residues.</text>
            <dbReference type="EC" id="3.4.21.122"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">The catalytically active form interacts with ACE2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="21">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.122" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4L101"/>
    <dbReference type="Ensembl" id="ENSEAST00005005717.2">
        <property type="protein sequence ID" value="ENSEASP00005005220.2"/>
        <property type="gene ID" value="ENSEASG00005003908.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155207"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000027">
        <property type="entry name" value="Transmembrane serine protease 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF30">
        <property type="entry name" value="TRANSMEMBRANE SERINE PROTEASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15494">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0720">Serine protease</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="13" id="KW-0865">Zymogen</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="24">
        <location>
            <begin position="176"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="23">
        <location>
            <begin position="272"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="20">
        <location>
            <begin position="151"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00064428"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00066608"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00071696"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005220.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="508" mass="55739" checksum="4702C5F485592D30" modified="2023-09-13" version="2">HLQPSKYLPLPDAADPVGMALNSGSPPGVGPYYENHGYQPESFYSRQPPVAPGAYGAYEAEYYPPTVPQYSPRVLTQASTPVNHTQPKLLSETVCTSKTKKVLCILLNLGLLLVALVVAAILLWKFVENKCLGSRIECGNSGICISASQWCDGILHCPNGEDENRCVRLYGLNFILQVYSSQRKSWHPVCEDDWSESYGRAACRDMGYGNSFYSSQGIKDDSGATSFMKLNTSADNIDLYKKLYHSDVCSSKKVVSLRCIECGVSSKQQSRIVGGANAALGEWPWQVSLHVQDVHVCGGSIITPEWIVTAAHCVEPPLNSPQYWTAFAGILSQSLMFYGNGHRVGKVIAHPNYDSKTKNNDIALMKLEAPLTFNDRVKPVCLPNPGMRLEPKQSCWISGWGATYEKGKTSDLLNAAMVPLIEPEQCNNKFIYNNLITPAMICAGYLEGNVDSCQGDSGGPLVTLKSSIWWLIGDTSWGSGCAKINRPGVYGNVTVFTDWIYQEMRANS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L153</accession>
    <name>A0A8C4L153_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ATP binding cassette subfamily G member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ABCG4</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014711161.1">
        <property type="nucleotide sequence ID" value="XM_014855675.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014711162.1">
        <property type="nucleotide sequence ID" value="XM_014855676.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070346209.1">
        <property type="nucleotide sequence ID" value="XM_070490108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L153"/>
    <dbReference type="Ensembl" id="ENSEAST00005005782.2">
        <property type="protein sequence ID" value="ENSEASP00005005280.2"/>
        <property type="gene ID" value="ENSEASG00005003953.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106840303"/>
    <dbReference type="KEGG" id="eai:106840303"/>
    <dbReference type="CTD" id="64137"/>
    <dbReference type="GeneTree" id="ENSGT00940000157853"/>
    <dbReference type="OrthoDB" id="66620at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034041">
        <property type="term" value="F:ABC-type sterol transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071403">
        <property type="term" value="P:cellular response to high density lipoprotein particle stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990830">
        <property type="term" value="P:cellular response to leukemia inhibitory factor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033344">
        <property type="term" value="P:cholesterol efflux"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045542">
        <property type="term" value="P:positive regulation of cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010875">
        <property type="term" value="P:positive regulation of cholesterol efflux"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03213">
        <property type="entry name" value="ABCG_EPDR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000267">
        <property type="entry name" value="ATP-binding cassette, sub-family G (WHITE), member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013525">
        <property type="entry name" value="ABC2_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043926">
        <property type="entry name" value="ABCG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050352">
        <property type="entry name" value="ABCG_transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48041">
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
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    <keyword evidence="10 12" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="396"/>
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            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
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            <dbReference type="Ensembl" id="ENSEASP00005005280.2"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L4Q1</accession>
    <name>A0A8C4L4Q1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">GPI alpha-1,4-mannosyltransferase I, catalytic subunit</fullName>
            <ecNumber evidence="13">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">GPI mannosyltransferase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PIGM</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
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                <person name="J."/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11 13">Catalytic subunit of the glycosylphosphatidylinositol-mannosyltransferase I complex which catalyzes the transfer of the first mannose, via an alpha-1,4 bond from a dolichol-phosphate-mannose (Dol-P-Man) to the glucosaminyl acyl phosphatidylinositol (GlcN-(acyl)PI) intermediate to generate alpha-D-Man-(1-&gt;4)-alpha-D-GlcN-(1-&gt;6)-(1-radyl,2-acyl-sn-glycero-3-phospho)-2-acyl-inositol and participates in the sixth step of the glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Endoplasmic reticulum membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the PIGM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4L4Q1"/>
    <dbReference type="Ensembl" id="ENSEAST00005004624.2">
        <property type="protein sequence ID" value="ENSEASP00005004219.2"/>
        <property type="gene ID" value="ENSEASG00005003195.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017728"/>
    <dbReference type="UniPathway" id="UPA00196"/>
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        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990529">
        <property type="term" value="C:glycosylphosphatidylinositol-mannosyltransferase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180041">
        <property type="term" value="F:dol-P-Man:GlcN-acyl-PI alpha-1,4-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007704">
        <property type="entry name" value="PIG-M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886:SF0">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12886">
        <property type="entry name" value="PIG-M MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05007">
        <property type="entry name" value="Mannosyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13" id="KW-0328">Glycosyltransferase</keyword>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="333"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="389" mass="45415" checksum="C15A6EAAC085999D" modified="2023-09-13" version="2">MVPTKHWGEWFLNLRVSPLGVFGVAFLARVALVFYGVFQDRTLLVRYTDIDYQVFTDAARFITEGRSPYLRVTYRYTPLLGWLLTPNIYLNELFGKFLFISCDLLTAFLQYRLLLLKGLGRRQACGYCVFWLLNPLPMAVSSRGSADAIVASLVLMALYLIEKRLVACAAVFYGFAVHMKIYPVTYILPIALHLLPERDNDEGLRQSRYSFKARLCEFLKRLCNRAVLLFVGVAGLTFFALSFGFYYKYGWEFLQHTYLYHLTRRDIRHNFSPYFYMLYLTAESKWSFSLGIAAFLPQFILLSAVSFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLVRMPWKRAVVLLLLWFIGQALWLAPAYVLEFQGQNTFLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L5Z0</accession>
    <name>A0A8C4L5Z0_EQUAS</name>
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        <submittedName>
            <fullName evidence="3">ENTH domain containing 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ENTHD1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
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                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="Ji"/>
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                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
                <person name="J."/>
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                <person name="A."/>
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                <person name="M."/>
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                <person name="D.D."/>
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            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="ENTH DOMAIN-CONTAINING PROTEIN 1"/>
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        <location>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="465"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="614" mass="68038" checksum="4F353C5D293FF43B" modified="2023-09-13" version="2">MAFRRQVKNFVKNYSDAEIKVREATSNDPWGPSSSLMLDISDLTFNTISLSEIMNMLWQRLKDHGKNWRHVYKSLTLMDYLIKNGSRKVIQHCREGFCNLQTLKDFQHVDEAGKDQGYYIREKSKQVITLLMDEQLLHKEREIACRTRRRTSYSMTFPKRVPGTGNSPTACASAPTPEIPASERKHKLLKVARLCNKKNASKAGLKQQQCQDAQLPSGTALSQETLPFKINAWKSTEDLMLFCEDDPQPLLPAIPPAILSPTTWLSEGQADICHLWDADAVSPPSFVLSEKSPSLQTNVSLDKKSESTITNTVIENPLQTPLEKQSAAKSFETLTTLPAFWPSNQEELISPNLRLSKSDSTFYNQASVETLYVSPSFNVFDPVKEMVINKDCQKPAESSVVQMEEENLKTLTAWVSTASEGTSSFSTLSMSSPDSASPGKSVHLLPPILARPSFWTLSHQTSSSASFKDKDKTTSAHHPFAPGGPVSSDEEENDNLNLLEILPDNSDSAQKKSHISSSNWVEFSTQNVDPFTSMSCSGFQTSKGRPREPEAKNSIKGLLGEVKNAIVKLHEDLSMVIQELNVINSHLVSMGGASPQISRSLQLPQSSEGGSDPV</sequence>
</entry>
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    <accession>A0A8C4L628</accession>
    <name>A0A8C4L628_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Acyl-CoA thioesterase 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ACOT9</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
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                <person name="Zhao"/>
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                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
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                <person name="Yang"/>
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                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
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                <person name="Liu"/>
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                <person name="J."/>
                <person name="Pan"/>
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                <person name="G."/>
                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
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                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl coenzyme A hydrolase family.</text>
    </comment>
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    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00390000005330"/>
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        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="BFIT_BACH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000012">
        <property type="entry name" value="Acyl-coenzyme A thioesterase 9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000016">
        <property type="entry name" value="Acyl-coenzyme A thioesterase 9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033120">
        <property type="entry name" value="HOTDOG_ACOT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12655:SF0">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51770">
        <property type="entry name" value="HOTDOG_ACOT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="HotDog ACOT-type" evidence="9">
        <location>
            <begin position="86"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="HotDog ACOT-type" evidence="9">
        <location>
            <begin position="289"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01106"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="439" mass="50118" checksum="1C349B2E0A9AA9F5" modified="2023-09-13" version="2">MRRAALRLCTLNKGLLTPARGLTQGSQNPEKQNVFHILEVRDKLREIVGASTNWRDHVKAMEERKLLQSFLAKSQEGLPPRRMKDSYIEVLLPLGSQPELREKYLTVQNTVRFGRILEDLDSLGVLVCYMHTRIHSAKMSPLSIVTALVDKIDLCKKNLSPEQDIKFSGHVSWVGKTSMEVKMRMFQLHGNEFCPVLDATFVMVARDSENKGPAFVNPLIPESPEEEELFRQGELNKGRRVAFSSTSLLKMAPSAEERTTIHEMFLSTLDPKTISFRSRILPPNAMWMENSKLKSLEICHPQERNIFNRIFGGFLMRKAYELGWATACNFGGSRPFVVAVDDIMFQKPVEVGSLLFLSSQVCFTQGNYIQVRVHSEVASLQNKEHMTTNVFHFTFMSEKEVPLVCPRTYGESMLYLDGQRHFNSMSLPVNLRKDFLVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L697</accession>
    <name>A0A8C4L697_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106837803</name>
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    <organism evidence="17 18">
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        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
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                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Cell membrane</location>
            <topology evidence="2 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L697"/>
    <dbReference type="Ensembl" id="ENSEAST00005004544.2">
        <property type="protein sequence ID" value="ENSEASP00005004143.2"/>
        <property type="gene ID" value="ENSEASG00005003141.2"/>
    </dbReference>
    <dbReference type="KEGG" id="eai:106837803"/>
    <dbReference type="CTD" id="8590"/>
    <dbReference type="GeneTree" id="ENSGT01140000282520"/>
    <dbReference type="OrthoDB" id="5967130at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15224">
        <property type="entry name" value="7tmA_OR6B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF359">
        <property type="entry name" value="ODORANT RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="62"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="278"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="42"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005004143.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="327" mass="36113" checksum="25FE4BEB3FFDB4BB" modified="2023-09-13" version="2">MEQRNQSGRVSEFVLLGFPAPVPLRTLLFALSLLAYVLVLTENTLIIMAVRNHPTLHKPMYFFLANMSFLEIWYVTVTIPKMLAGFTGSKQGHGQLISFEGCMTQLYFFLGLGCTECVLLAVMAYDRYVAICHPLHYPVIVSGRLCVQLATGSWAGGFGISMVKVFLISRLSYCGPNIINHFFCDVSPLLNLSCTDMSTAELTDFVLAIFILLGPLSVTGASYMAITHAVMHIPSAAGRHKAFSTCASHLTVVVIFYAASIFIYARPKALSAFDTNKLVSVLYAVIVPLLNPIIYCLRNQEVKRALRRTLHLYQGQDAKPGKISRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L6P8</accession>
    <name>A0A8C4L6P8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Vesicular glutamate transporter 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="28">Solute carrier family 17 member 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="33" type="primary">SLC17A7</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>3 Na(+)(out) + phosphate(out) = 3 Na(+)(in) + phosphate(in)</text>
            <dbReference type="Rhea" id="RHEA:71255"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>K(+)(in) + H(+)(out) = K(+)(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:29467"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>L-glutamate(out) = L-glutamate(in)</text>
            <dbReference type="Rhea" id="RHEA:66336"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>phosphate(in) = phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:32823"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Synaptic vesicle membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Synapse</location>
            <location evidence="22">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="26">Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L6P8"/>
    <dbReference type="Ensembl" id="ENSEAST00005007395.2">
        <property type="protein sequence ID" value="ENSEASP00005006769.2"/>
        <property type="gene ID" value="ENSEASG00005004995.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159110"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044300">
        <property type="term" value="C:cerebellar mossy fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060076">
        <property type="term" value="C:excitatory synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048786">
        <property type="term" value="C:presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008068">
        <property type="term" value="F:extracellularly glutamate-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140788">
        <property type="term" value="F:L-glutamate uniporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005326">
        <property type="term" value="F:neurotransmitter transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140787">
        <property type="term" value="F:phosphate ion uniporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005436">
        <property type="term" value="F:sodium:phosphate symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007616">
        <property type="term" value="P:long-term memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098700">
        <property type="term" value="P:neurotransmitter loading into synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001504">
        <property type="term" value="P:neurotransmitter uptake"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055062">
        <property type="term" value="P:phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050803">
        <property type="term" value="P:regulation of synapse structure or activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044341">
        <property type="term" value="P:sodium-dependent phosphate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035249">
        <property type="term" value="P:synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097401">
        <property type="term" value="P:synaptic vesicle lumen acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd17382">
        <property type="entry name" value="MFS_SLC17A6_7_8_VGluT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000004">
        <property type="entry name" value="vesicular glutamate transporter 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000005">
        <property type="entry name" value="vesicular glutamate transporter 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF29">
        <property type="entry name" value="VESICULAR GLUTAMATE TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0868">Chloride</keyword>
    <keyword evidence="16" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="20" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="19" id="KW-0407">Ion channel</keyword>
    <keyword evidence="14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15 31" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0532">Neurotransmitter transport</keyword>
    <keyword evidence="6" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0915">Sodium</keyword>
    <keyword evidence="17" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="10" id="KW-0769">Symport</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="7" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="8 31" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 31" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="140"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="283"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="381"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="417"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="456"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="480"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="513"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="31">
        <location>
            <begin position="545"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="32">
        <location>
            <begin position="139"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="574"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="30">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="30">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="30">
        <location>
            <begin position="597"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="30">
        <location>
            <begin position="608"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="30">
        <location>
            <begin position="627"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
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            <dbReference type="ARBA" id="ARBA00023053"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024167"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
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            <dbReference type="ARBA" id="ARBA00034102"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00035839"/>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00036616"/>
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    </evidence>
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    <evidence type="ECO:0000256" key="26">
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            <dbReference type="ARBA" id="ARBA00038044"/>
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    </evidence>
    <evidence type="ECO:0000256" key="27">
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            <dbReference type="ARBA" id="ARBA00039390"/>
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    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00041351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00047912"/>
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    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006769.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="637" mass="69134" checksum="17FDAAA94F2FAC47" modified="2023-09-13" version="2">MRGAGSSRDGRGLSPPRPAPDAECSGSPPAASTRRLLPPERSCGAGRAGADPGDPDAAARARGRGDQRGVPAAGAATMEFRQEEFRKLAGRALGRLHRLLEKRQEGAETLELSADGRPVTTQTRDPPIVDCTCFGLPRRYIIAIMSGLGFCISFGIRCNLGVAIVSMVNNSTTHRGGHVVVQKAQFNWDPETVGLIHGSFFWGYIVTQIPGGFICQKFAANRVFGFAIVATSTLNMLIPSAARVHYGCVIFVRILQGLVEGVTYPACHGIWSKWAPPLERSRLATTAFCGSYAGAVVAMPLAGVLVQYSGWSSVFYVYGSFGIFWYLFWLLVSYESPALHPSISEEERKYIEDAIGESAKLMNPVTKFNTPWRRFFTSMPVYAIIVANFCRSWTFYLLLISQPAYFEEVFGFEISKVGLVSALPHLVMTIIVPIGGQIADFLRSRRIMSTTNVRKLMNCGGFGMEATLLLVVGYSHSKGVAISFLVLAVGFSGFAISGFNVNHLDIAPRYASILMGISNGVGTLSGMVCPIIVGAMTKHKTREEWQYVFLIASLVHYGGVIFYGVFASGEKQPWAEPEEMSEEKCGFVGHDQLAGSDESDMEDEAEPPGAPPAPPPSYGATHSTVQPPRPPPPVRDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LB91</accession>
    <name>A0A8C4LB91_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">E3 ubiquitin-protein ligase AMFR</fullName>
            <ecNumber evidence="12">2.3.2.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Autocrine motility factor receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">AMFR</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-S-ubiquitinyl-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.36" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4LB91"/>
    <dbReference type="Ensembl" id="ENSEAST00005007217.2">
        <property type="protein sequence ID" value="ENSEASP00005006599.2"/>
        <property type="gene ID" value="ENSEASG00005004875.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156482"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036513">
        <property type="term" value="C:Derlin-1 retrotranslocation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904288">
        <property type="term" value="F:BAT3 complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990381">
        <property type="term" value="F:ubiquitin-specific protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034450">
        <property type="term" value="F:ubiquitin-ubiquitin ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007249">
        <property type="term" value="P:canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038061">
        <property type="term" value="P:non-canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044314">
        <property type="term" value="P:protein K27-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000638">
        <property type="term" value="P:regulation of SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14421">
        <property type="entry name" value="CUE_AMFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16455">
        <property type="entry name" value="RING-H2_AMFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000026">
        <property type="entry name" value="E3 ubiquitin-protein ligase AMFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000149">
        <property type="entry name" value="E3 ubiquitin-protein ligase AMFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040675">
        <property type="entry name" value="AMFR_Ube2g2-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003892">
        <property type="entry name" value="CUE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057992">
        <property type="entry name" value="TPR_SYVN1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067:SF5">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE AMFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15067">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02845">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18442">
        <property type="entry name" value="G2BR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25563">
        <property type="entry name" value="TPR_SYVN1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00546">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51140">
        <property type="entry name" value="CUE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="245"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="CUE" evidence="19">
        <location>
            <begin position="360"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="408"/>
            <end position="484"/>
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    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="502"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="457"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="469"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="513"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    <evidence type="ECO:0000256" key="11">
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            <dbReference type="ARBA" id="ARBA00034438"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069722"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
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            <dbReference type="ARBA" id="ARBA00076914"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006599.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="548" mass="62910" checksum="CF37853205BC436B" modified="2023-09-13" version="2">MLVAKLIQCIVFGPLRVSERQHLKDKFWNFIFYKFIFIFGVLNVQTVEEVVMWCLWFAGLVFLHLMVQLCKDRFEYLSFSPTTPMSSHGRVACDLCYLLSAVDLLCPVFELQAPIMHTVLCFVAAQSLLVTVRTAHVILRYVIHLWDLNHEGTWEGKGTYVYYTDFVMELTLLSLDLMHHIHMLLFGNIWLSMASLVIFMQLRYLFHEVQRRIRRHKNYLRVVGNMEARFAVATPEELAVNNDDCAICWDSMQAARKLPCGHLFHNSCLRSWLEQDTSCPTCRMSLNIADNNRAREDHQGENLDENLVPVAAAEGRPRLNQHNHFFHFDGSRIASWLPSFSVEVMHTTNILGITQASNSQLNAMAHQIQEMFPQVPYHLVLQDLQLTRSVEITTDNILEGRIQVPFPTQRSESIRPALNSPVERPNGDLEEGEPSAQTERVPLDLSPRLEEALDSMSEVEAEPSEGEDFEARGSRFSKSADERQRMLVQRKDDLLQQARKRFLNRSSEDDSASESCLPSEGATSDPVTLRRRMLAAAAERRLQKQQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LCB8</accession>
    <name>A0A8C4LCB8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Diencephalon/mesencephalon homeobox protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Orthodenticle homolog 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Paired-like homeobox protein DMBX1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">DMBX1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Homodimer or heterodimer. Forms heterodimers with OTX2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044626650.1">
        <property type="nucleotide sequence ID" value="XM_044770715.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LCB8"/>
    <dbReference type="Ensembl" id="ENSEAST00005007241.2">
        <property type="protein sequence ID" value="ENSEASP00005006622.2"/>
        <property type="gene ID" value="ENSEASG00005004889.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822591"/>
    <dbReference type="KEGG" id="eai:106822591"/>
    <dbReference type="CTD" id="127343"/>
    <dbReference type="GeneTree" id="ENSGT00940000155505"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008343">
        <property type="term" value="P:adult feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008344">
        <property type="term" value="P:adult locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048589">
        <property type="term" value="P:developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000125">
        <property type="entry name" value="diencephalon/mesencephalon homeobox protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052488">
        <property type="entry name" value="DMBX_homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003654">
        <property type="entry name" value="OAR_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46639">
        <property type="entry name" value="DIENCEPHALON/MESENCEPHALON HOMEOBOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46639:SF2">
        <property type="entry name" value="DIENCEPHALON_MESENCEPHALON HOMEOBOX PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03826">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50803">
        <property type="entry name" value="OAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="64"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="OAR" evidence="15">
        <location>
            <begin position="355"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="66"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="123"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="153"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="173"/>
            <end position="186"/>
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</entry>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">PPIases accelerate the folding of proteins during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
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        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
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        <property type="component" value="Chromosome 13"/>
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    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="4"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
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    <dbReference type="InterPro" id="IPR051989">
        <property type="entry name" value="FKBP-like_isomerase"/>
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        <property type="entry name" value="PPIase_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46046:SF3">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46046">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF13202">
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    <dbReference type="Pfam" id="PF00254">
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    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
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        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 13" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 13" id="KW-0697">Rotamase</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040137534" description="peptidylprolyl isomerase" evidence="14">
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            <begin position="27"/>
            <end position="582"/>
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            <begin position="62"/>
            <end position="150"/>
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    <feature type="domain" description="PPIase FKBP-type" evidence="15">
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            <begin position="399"/>
            <end position="486"/>
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            <begin position="497"/>
            <end position="532"/>
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    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="542"/>
            <end position="577"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="582" mass="64364" checksum="AD8B7DCB9F4F15D7" modified="2023-09-13" version="2">MFREGPPSHTLLRLPLLQLLLLLVQAVGRGLGRANPAGGPLEDVVIERYHIPRACPREVQRGDYVRYHYNGTFEDGKKFDSSYDRTTLVAIVVGVGRLITGMDRGLMGMCVSERRRLIVPPHLGYGSIGVAGLIPPDATLYFDVVLLDVWNKADTVQVSTLLRPPHCPRMVQDSDFVRYHYNGTLLDGTAFDTSYSRGGTYDTYVGSGWLIKGMDQGLLGMCPGERRKIIIPPFLAYGEKGYGTVIPAHASLVFHVLLIDVHNPKDTVQLETLELPPGCVRRAVAGDFMRYHYNGSLMDGTLFDSSYSRNHTYNTYVGQGYIIPGMDQGLQGACVGERRRITIPPHLAYGENGTGDKIPGSAVLVFDVHVIDFHNPADPVEVTTLSRPPEPCNETAQPGDFVRYHYNCSLLDGTRLFSSHDYEVPQEATLGANKVIEGLDKGLQGMCVGERRQLVVPPHLAHGESGARGVPGSAVLLFEVELVSREEGLPAGYLFVWHEDPPANLFEDLDLNKDGEVPPEEFSTFIKAQVSEGKGRLMPGQDPEKIIGDMFQNQDRNQDGKITAEELKLKSDEDQERVHEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LD73</accession>
    <name>A0A8C4LD73_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Mitochondrial dicarboxylate carrier</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Solute carrier family 25 member 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">SLC25A10</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
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                <person name="J."/>
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                <person name="R."/>
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                <person name="M."/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Catalyzes the electroneutral exchange or flux of physiologically important metabolites such as dicarboxylates (malonate, malate, succinate), inorganic sulfur-containing anions, and phosphate, across mitochondrial inner membrane. Plays an important role in gluconeogenesis, fatty acid metabolism, urea synthesis, and sulfur metabolism, particularly in liver, by supplying the substrates for the different metabolic processes. Regulates fatty acid release from adipocytes, and contributes to systemic insulin sensitivity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(S)-malate(in) + phosphate(out) = (S)-malate(out) + phosphate(in)</text>
            <dbReference type="Rhea" id="RHEA:71607"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(S)-malate(in) + succinate(out) = (S)-malate(out) + succinate(in)</text>
            <dbReference type="Rhea" id="RHEA:29327"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(S)-malate(in) + sulfate(out) = (S)-malate(out) + sulfate(in)</text>
            <dbReference type="Rhea" id="RHEA:71615"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>malonate(out) + (S)-malate(in) = malonate(in) + (S)-malate(out)</text>
            <dbReference type="Rhea" id="RHEA:71611"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:15792"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>malonate(out) + phosphate(in) = malonate(in) + phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:71623"/>
            <dbReference type="ChEBI" id="CHEBI:15792"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>malonate(out) + succinate(in) = malonate(in) + succinate(out)</text>
            <dbReference type="Rhea" id="RHEA:71667"/>
            <dbReference type="ChEBI" id="CHEBI:15792"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>succinate(out) + phosphate(in) = succinate(in) + phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:71627"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>sulfate(out) + phosphate(in) = sulfate(in) + phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:71631"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 24">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LD73"/>
    <dbReference type="Ensembl" id="ENSEAST00005008680.2">
        <property type="protein sequence ID" value="ENSEASP00005007958.2"/>
        <property type="gene ID" value="ENSEASG00005005784.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156783"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015140">
        <property type="term" value="F:malate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046166">
        <property type="term" value="P:glyceraldehyde-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000043">
        <property type="entry name" value="mitochondrial dicarboxylate carrier isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050391">
        <property type="entry name" value="Mito_Metabolite_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45618">
        <property type="entry name" value="MITOCHONDRIAL DICARBOXYLATE CARRIER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="9" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="11 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 23 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 24" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="23">
        <location>
            <begin position="8"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="23">
        <location>
            <begin position="101"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="23">
        <location>
            <begin position="197"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00051541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00057945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00072782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00076498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007958.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="297" mass="32388" checksum="C711B060850FCE35" modified="2023-09-13" version="2">MAAEARVSRWYFGGLASCGAACCTHPLDLLKVHLQTQQEVKMRMTGMALQVVRSDGVLALYNGLSASLCRQMTYSLTRFAIYETVRDHVTAGSQGPPPFYKKVLLGSISGCIGGFVGTPADMVNVRMQNDMKLPKNQRRNYAHALDGLYRVAREEGLKKLFSGATMASSRGVFVTVGQLSCYDQAKQLVLSTGYLSDSIVTHFVASFIAAAGDPPHPTQGGCATFLCQPLDVLKTRLMNSKGEYRGVFHCAVETAKLGPLAFYKGLLPAAIRLMPHTVLTFVFLEQLRKHFGIKVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LFS5</accession>
    <name>A0A8C4LFS5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Inner nuclear membrane protein Man1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">LEM domain-containing protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LEMD3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Can function as a specific repressor of TGF-beta, activin, and BMP signaling through its interaction with the R-SMAD proteins. Antagonizes TGF-beta-induced cell proliferation arrest.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with SMAD1, SMAD2, SMAD3 and SMAD5. Binds to both phosphorylated and unphosphorylated R-SMADS.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014694075.2">
        <property type="nucleotide sequence ID" value="XM_014838589.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LFS5"/>
    <dbReference type="Ensembl" id="ENSEAST00005012294.2">
        <property type="protein sequence ID" value="ENSEASP00005011307.2"/>
        <property type="gene ID" value="ENSEASG00005007930.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106829473"/>
    <dbReference type="KEGG" id="eai:106829473"/>
    <dbReference type="CTD" id="23592"/>
    <dbReference type="GeneTree" id="ENSGT00940000157643"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032926">
        <property type="term" value="P:negative regulation of activin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030512">
        <property type="term" value="P:negative regulation of transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006998">
        <property type="term" value="P:nuclear envelope organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12286">
        <property type="entry name" value="RRM_Man1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1180:FF:000001">
        <property type="entry name" value="inner nuclear membrane protein Man1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000176">
        <property type="entry name" value="Inner nuclear membrane protein Man1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.40:FF:000001">
        <property type="entry name" value="LEM domain containing 2, isoform CRA_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1180">
        <property type="entry name" value="MAN1, winged-helix domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052277">
        <property type="entry name" value="INM_ESCRT-Associated"/>
    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
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    <dbReference type="PANTHER" id="PTHR13428:SF10">
        <property type="entry name" value="INNER NUCLEAR MEMBRANE PROTEIN MAN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13428">
        <property type="entry name" value="INNER NUCLEAR MEMBRANE PROTEIN MAN1 LEM DOMAIN CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF03020">
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        <property type="entry name" value="MSC"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00540">
        <property type="entry name" value="LEM"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF63451">
        <property type="entry name" value="LEM domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50954">
        <property type="entry name" value="LEM"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="635"/>
            <end position="652"/>
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            <begin position="6"/>
            <end position="50"/>
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            <begin position="19"/>
            <end position="348"/>
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            <begin position="164"/>
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    <evidence type="ECO:0000313" key="15">
        <source>
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    <evidence type="ECO:0000313" key="16">
        <source>
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    <sequence length="911" mass="98247" checksum="C23EE302E8F5E1F3" modified="2023-09-13" version="2">MAAAAASAPQQLSDEELFSQLRRYGLSPGPVTESTRPVYLKKLKKLREEEQQQHRPGGRGSKTRNSNNNNTAAATGAAAGPAGAAAGLGGRPAPGDLSSLRTAGGLGRSAAAGPESPLGGLGAAAAAPTAGSKVLLGFSSDESDAEASPRDQAGGGGGRRDRAPLQPRALRPPAAPLAAGEGTHSTPAERRKPHSWWGARRPAAPETPAGRDGAAEGEGGAGEDGGDRDPEAEQPPWASRAVNGSRLLPYSCRENYSDSEEEDDADVASARQVLKDDPLSRPRPRRGHSKPLSPPPARAAGGRAEPSAPGGGGVALSDRAAAAGGLDRGRGLEEAAAAAAEQGGGCEPLDAGLVPRFRVTAKKLAPLLPPPAADADPASDPSTGSLLKTNNHVGGGAFGADAPRIFPNSLPPGAAAAASSSLRINHANHTGANHTYLKNAYGKPKLSEPEEELLQQFKREEVSPTGGFSAHYLSMFLLTAACLFFLILGLTYLGMRGTGVAEDGGLSIKNPFDETFGKIQESKKNLMMNTLYKLHDRLAQLAGDHECGSSSQRTLSVQEAAAYLKDLGSEYEDVFNTSLKWILENGKDVGIRCVGYGPEEELRNITDVQFLQSTRPQMSFWCRFRRAFITVTHRLLLLCLGVVMVCVVLRYMKYRWTKEEEETRQMYDMVVKIIDVLRSHNEACQENKDLQPYMPIPHVRDSLIQPHDRKKMKKVWDRAVDFLAANESRVRTETRRIGGADFLVWRWIQPSASCDKILVIPSKVWQGQAFHLDRRNSPPNSLTPCLKIRNMFDPVMEIGDQWHLAIQEAILEKCSDNDGIVHIAVDKNSREGCVYVKCLSPEYAGKAFKALHGSWFDGKLVTVKYLRLDRYHHRFPQALTCNTPLKPSNTHMNSMSHLRLRTGLANSQVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LFU3</accession>
    <name>A0A8C4LFU3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cytoplasmic polyadenylation element binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CPEB2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM CPEB family.</text>
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        <property type="protein sequence ID" value="ENSEASP00005007384.2"/>
        <property type="gene ID" value="ENSEASG00005005397.2"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0000900">
        <property type="term" value="F:mRNA regulatory element binding translation repressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008135">
        <property type="term" value="F:translation factor activity, RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032869">
        <property type="term" value="P:cellular response to insulin stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000766">
        <property type="term" value="P:negative regulation of cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd19757">
        <property type="entry name" value="Bbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12724">
        <property type="entry name" value="RRM1_CPEB2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12726">
        <property type="entry name" value="RRM2_CPEB2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000008">
        <property type="entry name" value="Cytoplasmic polyadenylation element-binding 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.640.40:FF:000001">
        <property type="entry name" value="Cytoplasmic polyadenylation element-binding 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000009">
        <property type="entry name" value="cytoplasmic polyadenylation element-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.40">
        <property type="entry name" value="Cytoplasmic polyadenylation element-binding protein, ZZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032296">
        <property type="entry name" value="CEBP_ZZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038446">
        <property type="entry name" value="CEBP_ZZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034819">
        <property type="entry name" value="CPEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
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        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12566">
        <property type="entry name" value="CYTOPLASMIC POLYADENYLATION ELEMENT BINDING PROTEIN CPEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12566:SF8">
        <property type="entry name" value="CYTOPLASMIC POLYADENYLATION ELEMENT-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16366">
        <property type="entry name" value="CEBP_ZZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16367">
        <property type="entry name" value="RRM_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
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        <location>
            <begin position="781"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="889"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="120"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="171"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="486"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="559"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="208"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="219"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
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            <begin position="283"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="319"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="353"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="382"/>
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        </location>
    </feature>
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        <location>
            <begin position="393"/>
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    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="501"/>
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            <end position="582"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007384.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1038" mass="110063" checksum="6E51DBAA31A419A7" modified="2023-09-13" version="2">MRDFGFGVLRTAPLRSSSPRSLFSGGAYCAYAAGPAAAAAPLPSATPFGPLSPPPLPVTGFSEGASPFSIPLGCGGAGSSAAGASSSSPFLAHQQTMQDELLLGLTQQPARPLSGAAAAEKLPNHHPGGGTNAGVTHLLPSQDFKPSLHHPSSSSASSCCCCRTSSPQDFSKRQQQQLSSQKRKEFSPPHLPHPPDSKQPPLHCPGRFSPPPPPPSPLLQPAQLAQRPQQQQQQQQQFSLPHPQHLPPQDFAPRQRPADLTPLPQLQPSPPAAPRRRHGGAGSPRKSPAAGEGSAAEPPNAGLAPSTPPVNPAPGSMESPNHPLLNSPSNLLPGGALGSGAFSSLQSPDLPHPGGGVGSGGGGPPGGGGGGGSASPPPPLPGFGTPWSVQTASPPPQPQPQPPPQPQPQPPPPQQPPQPPQPGSSTATPGGGGGGSLSAMPPPSPDSENGFYPGLPSSMNPAFFPSFSPVSPHGCAGLSVPASGGGGGGGGFGGPFSAAAVPPPPPPAMNLPQQQPPPPAAPQQPQSRRSPVSPQLQQQHQAAAAAFLQQRNSYNHHQPLLKQSPWSNHQSSGWGTGSMSWGAMHGRDHRRTGNMGIPGTMNQISPLKKPFSGNVIAPPKFTRSTPSLTPKSWIEDNVFRTDNNSNTLLPLQVRSSLQLPAWGSDSLQDSWCTAAGTSRIDQDRSRMYDSLNMHSLENSLIDIMRAEHDPLKGRLSYPHPGTDNLLMLNARSYGRRRGRSSLFPIDDGLLDDGHNDQVSVLNSPTCYSAHQNGERIERFSRKVFVGGLPPDIDEDEITASFRRFGPLVVDWPHKAESKSYFPPKGYAFLLFQEESSVQALIDACIEEDGKLYLCVSSPTIKDKPVQIRPWNLSDSDFVMDGSQPLDPRKTIFVGGVPRPLRAVELAMIMDRLYGGVCYAGIDTDPELKYPKGAGRVAFSNQQSYIAAISARFVQLQHGDIDKRVEVKPYVLDDQMCDECQGARCGGKFAPFFCANVTCLQYYCEFCWANIHSRAGREFHKPLVKEGADRPRQIHFRWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LIV9</accession>
    <name>A0A8C4LIV9_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Carboxypeptidase A2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CPA2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LIV9"/>
    <dbReference type="Ensembl" id="ENSEAST00005013720.2">
        <property type="protein sequence ID" value="ENSEASP00005012631.1"/>
        <property type="gene ID" value="ENSEASG00005008812.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160121"/>
    <dbReference type="OMA" id="WSYDSGI"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.340:FF:000001">
        <property type="entry name" value="Carboxypeptidase A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000001">
        <property type="entry name" value="Carboxypeptidase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.340">
        <property type="entry name" value="Metallocarboxypeptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057247">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036990">
        <property type="entry name" value="M14A-like_propep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003146">
        <property type="entry name" value="M14A_act_pep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705:SF71">
        <property type="entry name" value="CARBOXYPEPTIDASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11705">
        <property type="entry name" value="PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02244">
        <property type="entry name" value="Propep_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00765">
        <property type="entry name" value="CRBOXYPTASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00631">
        <property type="entry name" value="Zn_pept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00133">
        <property type="entry name" value="CARBOXYPEPT_ZN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M14" evidence="12">
        <location>
            <begin position="145"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005012631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="419" mass="47731" checksum="1B90F8AE4873A2BC" modified="2022-01-19" version="1">MDQPQPWFLQIKFRSDTEFWTLGRPLTMRLILFFGALFGYTYCRQTFVGHQILEMIPRNEEQIKNLTELEAEEHLQGSSPTIPGEIAHVRVPFSSIQAVKSFLESQETPYSVTIEDVQALLVKENEEMLFNLKTRERNGNFNFEAYHTFKEISEELDNLVAEHPGLLSKVNIGYSFEKRPLSVLKIHSTGGHKRAFWINARIHGREWITQATALWTANQIASDYGNDPSLTSILDAVDIFLLPVANPDGYVFSQTKKTWSEIPDRDCVGVDLNRNWDAHFGGPGASNDPCSHGYHGPSANSEVEVKSIVDLIKRHGNFKAFITLHSYSQLLVFPYGYTCSRPDNFNELVNFFHFYPSDQASGGSIDWACDFGIKYSFAFELRDTGCYGFLLPADQILPTAKETWLGLKTILEHVRDHPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LJJ3</accession>
    <name>A0A8C4LJJ3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Keratin 82</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">KRT82</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014702961.2">
        <property type="nucleotide sequence ID" value="XM_014847475.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LJJ3"/>
    <dbReference type="Ensembl" id="ENSEAST00005014089.2">
        <property type="protein sequence ID" value="ENSEASP00005012961.2"/>
        <property type="gene ID" value="ENSEASG00005009060.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835258"/>
    <dbReference type="KEGG" id="eai:106835258"/>
    <dbReference type="CTD" id="3888"/>
    <dbReference type="GeneTree" id="ENSGT00940000161849"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030280">
        <property type="term" value="F:structural constituent of skin epidermis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031424">
        <property type="term" value="P:keratinization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000004">
        <property type="entry name" value="Keratin, type II cytoskeletal 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032444">
        <property type="entry name" value="Keratin_2_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003054">
        <property type="entry name" value="Keratin_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616">
        <property type="entry name" value="GATA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616:SF12">
        <property type="entry name" value="KERATIN, TYPE II CUTICULAR HB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16208">
        <property type="entry name" value="Keratin_2_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01276">
        <property type="entry name" value="TYPE2KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="7">
        <location>
            <begin position="118"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="327"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005012961.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="518" mass="57261" checksum="F4DE3045906CEFCF" modified="2023-09-13" version="2">MSCRSFQQGSRCGSRNFSSNSAVLPRMVTHYAVSKGPFQLGGSGGLRALGCLGSRSLCNVGFGRPRVASRCSLPGFGYRLGATCGPSACIAPVTINESLLVPLELEIDPTVQQVKRDEKEQIKCLNNRFASFINKVRFLEQKNKLLETKWNFMQQQRCCQSNIEPIFEAYICALRRQLDCVAGDRARLESELCSLQDTLEGYKKKYEEELALRPCAENEFVALKKDVDTAFLTKADLETNVEALVQEIDFLKSLYEEEICLLQSQISETSVIVKMDNSRELDVDGIIAEIKAQYDEIASRSKAEAEAWYQCQYEELRLTAGNHCDNLRTRKNEILEMNKLIQRLQQDIENVKAQRCKLEATVTQAEQQGEAALTDAKCKLAGLEEALQKAKQDMACLLREYQEVMNSKLGLDIEIATYRRLLEGEEHRLCEGIGPMNISVSSSKGAILYEPCVVSTPVLRTEYCPGGAGVLKSSGSCSIVGTGELYIPCEPQGPLGCGSRRGPSTKLGAGGSSSCHKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LJS4</accession>
    <name>A0A8C4LJS4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106843452</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 14">Cell membrane</location>
            <topology evidence="3 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LJS4"/>
    <dbReference type="Ensembl" id="ENSEAST00005013447.2">
        <property type="protein sequence ID" value="ENSEASP00005012382.2"/>
        <property type="gene ID" value="ENSEASG00005030724.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106843452"/>
    <dbReference type="KEGG" id="eai:106843452"/>
    <dbReference type="GeneTree" id="ENSGT01050000244828"/>
    <dbReference type="OrthoDB" id="9830543at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15227">
        <property type="entry name" value="7tmA_OR14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000037">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="235"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005012382.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="312" mass="35025" checksum="05A0EBD9BCEEF807" modified="2023-09-13" version="2">MANFTTMNGFLLMGFSAKPELEILCASLFLVSYLVALTGNILIITATSMDDSLYSPMYFFLKHLSFLDLCYISVTVPRYICNSFMHNGNISLWECILQCFAFTLCGCAEMAMLTVMSYDRYVAICLPLHYEIIMNFNTCIHGILAVWISGVISGVMHTAATFSIHFCGTKIIHQFFCDVPQILKLSCSNEYVSELGVIVFLSLVAFLCFICIGLSYMHIFCTVLQIPSAEGRAKAFSTCVPHLSVVILFISTGVFAFLKPHSDSPTALDFLLSVFYTVLPPTLNPMIYSLRNKAMKTALRKVFKRRKAYFFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LKH8</accession>
    <name>A0A8C4LKH8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Nuclear receptor coactivator 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">NCOA6</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
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            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="302"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="302"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014524.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="339" mass="39968" checksum="C9399B000495F5DB" modified="2023-09-13" version="2">MFLQPTRISQGSAKDQPENLRSSQDFQMPWKQEAEFIKRDKAARVIQKAWKSFLNVAVFQHFKSLINIRRQGEPRHIMRYINPKEAELLDAAAGIHVRFRLGGVKFPPEIYYKIFTHRHIEDLCANSPRDYTKLPVKHTSHIKSNNPQEEDQSGWYRRTENNGWRPVSDRFWMSTEHVMMGDEKESEFHFSKLKRRQDMEKKRKIRKIEWMRQMYHAGSLEAKSTNRETLGLIHTATKGLIRAIEDGGIDSVMEWEVDEVLNWTNTLNFDEYIANWKETATSNSSDNFKSFRFDQAQKNIDDYEDLSEDEMETSADTSHRNSFDEPNFTRLTPDSTYGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LPW7</accession>
    <name>A0A8C4LPW7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pre-rRNA-processing protein TSR1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TSR1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Required during maturation of the 40S ribosomal subunit in the nucleolus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. TSR1 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LPW7"/>
    <dbReference type="Ensembl" id="ENSEAST00005014490.2">
        <property type="protein sequence ID" value="ENSEASP00005013342.1"/>
        <property type="gene ID" value="ENSEASG00005009302.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153195"/>
    <dbReference type="OMA" id="MNLPRFK"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030387">
        <property type="entry name" value="G_Bms1/Tsr1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF1">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22298">
        <property type="entry name" value="Tsr1_G-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51714">
        <property type="entry name" value="G_BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Bms1-type G" evidence="8">
        <location>
            <begin position="103"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="22"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="68"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00040070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005013342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="826" mass="94448" checksum="E0BE3E73608C8FC8" modified="2022-01-19" version="1">MGNRKRSIWPGSSAVSVHANGGMAAHRSGPLKQQNKPHKGGRHRGRGSAQRDGKGRLALKTLSKKLKKELSRVDQRHRASQLRKQKKEAVLAEKRQLGSKDGSPHQVLVVPLHNRISLTEAFRLLQDRDTGTVHLNEWGSSHGFMLLCPRLKHRWFFTSARPGDLHTVLDMAKVADTILFLLDPLEGWDSTGDYCLSCLFAQGLPTYTLAVQGVSGLPPKKQIDARKKLSKVVEKRFPDDKFLLLDTHQEAGMLLRQLANQKQRHLAFRDRRAYLLAHAADFVPGEENNLVGTLKISGYVRGQTLNVNSLLHIIGHGDFQMKQIDAPMDPFPLHPRVIKSWKDPGMAMEICAMDAVADMEEDVKVLMKADPDKQESLQTEVIPDPMEGEQTWPTEEELSEANDFLKERSKVVKKVPKGTSSYQAEWILDEDGESGGEGDEYDDIEQEDFMEEESQDESSEKEEEEEYETMTIGESVRDDLYDEKVDEEAEEKMLEKYKQERMEEMFPDEVDTPRDVAARIRFQKYRGLKSFRTSPWDPKENLPQDYARIFQFQNFTSTRRRIFKEIEEKELEGAEVGWYVTLHVSEVPVSVVEHFKRGAPLIAFSLLPHEQKMSVLNMVVSRHPGNTEPVKAKEELIFHCGFRRFRASPLFSQHTAADKHKFQRFLTADVALVVTVYAPITFPPASVLLFKQNSNGMHSLIATGYLLSVDPDRMVIKRVVLSGHPFKIFTKMAVVRYMFFSREDVLWFKPVELRTKWGRRGHIKEPLGTHGHMKCSFDGKLKSQDTVLMNLYKRVFPKWTYDPYVPEPVPWVKSGISSALPEVDME</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LTD3</accession>
    <name>A0A8C4LTD3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LTD3"/>
    <dbReference type="Ensembl" id="ENSEAST00005018209.2">
        <property type="protein sequence ID" value="ENSEASP00005016756.2"/>
        <property type="gene ID" value="ENSEASG00005033832.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163708"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042605">
        <property type="term" value="F:peptide antigen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04983">
        <property type="entry name" value="IgV_TCR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051006">
        <property type="entry name" value="TCR_variable_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19343">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19343:SF13">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-1279">T cell receptor</keyword>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="21"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005016756.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="112" mass="12308" checksum="05BB2C8251CB2B95" modified="2023-09-13" version="2">MDKPLSLLILWLQLDWVSSKQQVEQSPEALSVREGDSLALNCSYTDSAIYSLQWFRQDPGKGLASLLLIQSNQREQISGRMKASLDESSRHSALHIAASQPGDSATYLCAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LXH2</accession>
    <name>A0A8C4LXH2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106845117</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4LXH2"/>
    <dbReference type="Ensembl" id="ENSEAST00005016309.2">
        <property type="protein sequence ID" value="ENSEASP00005014988.2"/>
        <property type="gene ID" value="ENSEASG00005010487.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164247"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060816">
        <property type="term" value="P:random inactivation of X chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058896">
        <property type="entry name" value="RNF6/12_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF1">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25914">
        <property type="entry name" value="RNF6_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="E3 ubiquitin-protein ligase RNF6/12 N-terminal" evidence="12">
        <location>
            <begin position="17"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="67"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="104"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="407"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="502"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="104"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="135"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="159"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="207"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="407"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="502"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014988.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="619" mass="68895" checksum="5047C4774AC081C7" modified="2023-09-13" version="2">MENSDSDDGGGPAAQSGTEMDRLAHEDDFYRFVNNLSEEDYKLMRDNNLLGSPGESTEEELLRRLQLIKENSPQTSHDNTGGEDSLDNVSNGDYVKDWLNSFEPTENMTSGQRESQSWREVSQIHPKNDDFRFSSEMNFNLNNESPNPENDYAPSTRLPRGEENSQRQVENPRSESTRRRPSRSERSTTEALMEVPPTRGQRRARSRSPDGLRTRARTESWSPLKSPPQYEIFQRFCHRTPSQTFEPPLVNETQRFSRTQHRETLRQQITGLELQNRGLFATSGTRNAIQGERSPDTTSMNGESWELRQINPTIPFELEVGHVHPGAYPHRDSTASRTQLTSETPNNTITLESEHGGLGCMSSHCEQADARAYVSPVRIPVHRISNTGLNDTIPVAVQSTLSQTMTGFSDSSNLTDSYSDLEPSVSPPSQNMERADSLIGTDDSAATSSSGSNPYPCCDSHSTSTLTACSSCAYISSSSCTSICSSCDENSEISSLLFEGSDIGSLSSDSPSETRQESRQMTPITFDESDSWSSLNEDRFFLLNEDDYQSTGLTVAQTDNLALRSFSENNPSKSCSICITEYTERIVNYASYLAPMNIMSTVSLAGWLRTRPVLFVAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M1S3</accession>
    <name>A0A8C4M1S3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">N-acylneuraminate cytidylyltransferase</fullName>
            <ecNumber evidence="5">2.7.7.43</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">CMP-N-acetylneuraminic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CMAS</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the activation of N-acetylneuraminic acid (NeuNAc) to cytidine 5'-monophosphate N-acetylneuraminic acid (CMP-NeuNAc), a substrate required for the addition of sialic acid. Has some activity toward NeuNAc, N-glycolylneuraminic acid (Neu5Gc) or 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an N-acylneuraminate + CTP = a CMP-N-acyl-beta-neuraminate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11344"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:60073"/>
            <dbReference type="ChEBI" id="CHEBI:68671"/>
            <dbReference type="EC" id="2.7.7.43"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-sugar metabolism; N-acetylneuraminate metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer; the active enzyme is formed by a dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CMP-NeuNAc synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.43" evidence="5"/>
    <dbReference type="RefSeq" id="XP_014687932.1">
        <property type="nucleotide sequence ID" value="XM_014832446.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M1S3"/>
    <dbReference type="Ensembl" id="ENSEAST00005022041.2">
        <property type="protein sequence ID" value="ENSEASP00005020301.2"/>
        <property type="gene ID" value="ENSEASG00005013969.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106825570"/>
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        <property type="term" value="F:N-acylneuraminate cytidylyltransferase activity"/>
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        <property type="entry name" value="N-ACYLNEURAMINATE CYTIDYLYLTRANSFERASE"/>
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    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
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    <keyword evidence="7" id="KW-0808">Transferase</keyword>
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            <begin position="1"/>
            <end position="42"/>
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            <dbReference type="ARBA" id="ARBA00032878"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="434" mass="48443" checksum="2AE50C522404B611" modified="2023-09-13" version="2">MDSVEKGAATSVSNPRGRPSRGRPPKLQRNSRGGQGRGVEKPPHLAALILARGGSKGIPLKNIKHLAGVPLIGWVLRAALDSGVFQSVWVSTDHDEIENVAKQFGAQVHRRSSETSKDSSTSLDAIIEFLNFHNEVDIVGNIQATSPCLHPTDLQKVAEMIREEGYDSVFSVVRRHQFRWSEIQKGVREVTEPLNLNPAKRPRRQDWDGELYENGSFYFAKRHLIEMGYLQGGKMAYYEMRAEHSVDIDVDIDWPIAEQRVLRYGYFGKEKLKEIKLLVCNIDGCLTNGHIYVSGDQKEIISYDIKDAIGISLLKKSGIEVRLISERACSKQTLSSLKLDCKMEVNVPDKLAVVDEWRKEMGLCWKEVAYLGNEVSDEECLKKAGLSGVPADACSTAQKAVGYICKSNGGRGALREFAEHIFLLMEKVINSCQK</sequence>
</entry>
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    <name>A0A8C4M4Y0_EQUAS</name>
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        <recommendedName>
            <fullName evidence="2">DUF4629 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSEASG00005013799.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 6"/>
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        <property type="entry name" value="C2orf78-like"/>
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        <property type="entry name" value="DUF4629"/>
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    <dbReference type="PANTHER" id="PTHR31466">
        <property type="entry name" value="GENE 5591-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31466:SF1">
        <property type="entry name" value="RIKEN CDNA 4930433I11 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15442">
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4629" evidence="2">
        <location>
            <begin position="448"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="423"/>
            <end position="777"/>
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    </feature>
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        <location>
            <begin position="807"/>
            <end position="843"/>
        </location>
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        <location>
            <begin position="388"/>
            <end position="403"/>
        </location>
    </feature>
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        <location>
            <begin position="493"/>
            <end position="503"/>
        </location>
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        <location>
            <begin position="599"/>
            <end position="615"/>
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    </feature>
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        <location>
            <begin position="707"/>
            <end position="721"/>
        </location>
    </feature>
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            <end position="753"/>
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            <begin position="768"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="825"/>
            <end position="843"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020014.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="872" mass="93618" checksum="195A74DF67519B37" modified="2023-09-13" version="2">MLLLRHCQKNFQNPSLLGTPNSLQLSLPVVSDAASLAGSVCNFSRVSAPAVSSGWLLPSASGTSSQPLMGSAYLYQHSSTTMLSGVTGQSQSSTSAASYPGLFEWGVPGSTAQESSSLGDCTLTVTGHDTTVSSMSMAAHYDRTSGANNPVPLYPSLSTSLVQGTPSQVPNQGHSLSLPYQEGSQVYYYGHGTLGPLLSGELGPCLQSYGSVSYTGAGASAPQPEMVLVLKEIQPTHVLPPASTSGVYYACPTPPHTNGLPLETFLGLQPPSQTFCLQQTPEFPKSCSSRNTQMLQSNPPHEPADISLTASLQTSNSNLLALSPAPKNWDEFNTNLSKPLDPDQIPIENQDPLPHPLEIPDILQLLACIDPLGPEDHPASENADLGKSSLSLEGQGTLENGTEASGGFADIATLMEDGHLPQLFDPLKDLDQPQGPELIQSSGRKAASDNTRKNKHQASEPLDGAPKAKIQPKDPECLSGGEGLICNAAASDRAPENEAEHSNSKPQKAAPSRISTTKSHGQERTKKTRGNNPKKAGESRQSGTEVRVEEKPMMPRMKRRKNQPELSQETFKRPRTSLGQHMLESVQVFHALGRKSEKNTGPSSSRALGTSSNPKHPQPCPAIKPWLDKPLEGQCPEETQVKAQKAESSAEMECPPPSRDELPPPGKVRLVPLPFLSVDKPPARPVPRRPQALASHRPAVADPARPASTNSAQPTAVNSTHPAPASLTGPARPARPITTNPARPGWTNPTRPSGPQCPASRPAPYRTASCTSLQQEPVPTAVPMLQAPPKPPAQYLLEDFSKQPIPWRKPVIPGPVMSEPITQEQRPEREAMKRQAQQEREKAAKYTSWGKVQFFIEREKEMEISRYYGYAI</sequence>
</entry>
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    <accession>A0A8C4M6J0</accession>
    <name>A0A8C4M6J0_EQUAS</name>
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        <recommendedName>
            <fullName evidence="2 10">AP-2 complex subunit mu</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">AP2M1</name>
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    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
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    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="X."/>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <location evidence="10">Coated pit</location>
            <topology evidence="10">Peripheral membrane protein</topology>
            <orientation evidence="10">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4M6J0"/>
    <dbReference type="SMR" id="A0A8C4M6J0"/>
    <dbReference type="Ensembl" id="ENSEAST00005022707.2">
        <property type="protein sequence ID" value="ENSEASP00005020923.2"/>
        <property type="gene ID" value="ENSEASG00005014278.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159223"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097718">
        <property type="term" value="F:disordered domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005048">
        <property type="term" value="F:signal sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903077">
        <property type="term" value="P:negative regulation of protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098884">
        <property type="term" value="P:postsynaptic neurotransmitter receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097494">
        <property type="term" value="P:regulation of vesicle size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048488">
        <property type="term" value="P:synaptic vesicle endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd09251">
        <property type="entry name" value="AP-2_Mu2_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14836">
        <property type="entry name" value="AP2_Mu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1170:FF:000008">
        <property type="entry name" value="AP-2 complex subunit mu isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000002">
        <property type="entry name" value="AP-2 complex subunit mu, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043532">
        <property type="entry name" value="AP2_Mu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043512">
        <property type="entry name" value="Mu2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00990">
        <property type="entry name" value="CLAT_ADAPTOR_M_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00991">
        <property type="entry name" value="CLAT_ADAPTOR_M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0168">Coated pit</keyword>
    <keyword evidence="5 10" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="7 11" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="12">
        <location>
            <begin position="172"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="345"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="347"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="356"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4,5-trisphosphate)</name>
            <dbReference type="ChEBI" id="CHEBI:57836"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR005992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020923.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="437" mass="49925" checksum="FB0FBC75FF6F9E6B" modified="2023-09-13" version="2">MIGGLFIYNHKGEVLISRVYRDDIGNRRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHVKRSNIWLAAVTKQNVNAAMVFEFLYKMCDVMAAYFGKISEENIKNNFVLIYELLDEILDFGYPQNSETGALKTFITQQGIKSQHQTKEEQSQITSQVTGQIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M6S6</accession>
    <name>A0A8C4M6S6_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">IQ motif containing F1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">IQCF1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M6S6"/>
    <dbReference type="Ensembl" id="ENSEAST00005020278.2">
        <property type="protein sequence ID" value="ENSEASP00005018687.2"/>
        <property type="gene ID" value="ENSEASG00005012881.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004641"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000014">
        <property type="entry name" value="IQ motif containing F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.190:FF:000015">
        <property type="entry name" value="IQ motif containing F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039887">
        <property type="entry name" value="IQCF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21633:SF14">
        <property type="entry name" value="IQ DOMAIN-CONTAINING PROTEIN F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21633">
        <property type="entry name" value="IQ MOTIF CONTAINING F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005018687.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="207" mass="24210" checksum="8BDD68BFF2041935" modified="2023-09-13" version="2">VEKDQPKKINEHTKDEPQEKGELNGLSSAPLTSEKNMEANPMNMVETKRVDDANKTPREITGNQNKPPTSDPEVVKIQAWWRGTLVRRTLLHAALRALIIQNWWRQKLVKLLEDKRRMALETFIQREWAAVRLQSWVRMWRIHRRYCCLLNAARIIQAYWRCHSCATQGFIKGHYRVTANQLHLELEILLGSEPCIVTECIPLPIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MBK1</accession>
    <name>A0A8C4MBK1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C-type lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106834107</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MBK1"/>
    <dbReference type="Ensembl" id="ENSEAST00005023363.2">
        <property type="protein sequence ID" value="ENSEASP00005021525.2"/>
        <property type="gene ID" value="ENSEASG00005014731.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008117"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03593">
        <property type="entry name" value="CLECT_NK_receptors_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013600">
        <property type="entry name" value="Ly49_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052013">
        <property type="entry name" value="Mouse_KLRs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033992">
        <property type="entry name" value="NKR-like_CTLD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46329">
        <property type="entry name" value="KILLER CELL LECTIN-LIKE RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46329:SF1">
        <property type="entry name" value="KILLER CELL LECTIN-LIKE RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08391">
        <property type="entry name" value="Ly49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0430">Lectin</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="76"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="13">
        <location>
            <begin position="191"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022889"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022968"/>
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    </evidence>
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    </evidence>
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    </evidence>
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    <evidence type="ECO:0000313" key="15">
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MBN3</accession>
    <name>A0A8C4MBN3_EQUAS</name>
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        <recommendedName>
            <fullName evidence="3">Tudor domain-containing protein 5</fullName>
        </recommendedName>
    </protein>
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        <name evidence="14" type="primary">TDRD5</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Required during spermiogenesis to participate in the repression transposable elements and prevent their mobilization, which is essential for the germline integrity. Probably acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for chromatoid body (CB) assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDRD5 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014845287.3"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_044758256.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MBN3"/>
    <dbReference type="Ensembl" id="ENSEAST00005026578.2">
        <property type="protein sequence ID" value="ENSEASP00005024501.2"/>
        <property type="gene ID" value="ENSEASG00005016637.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106833925"/>
    <dbReference type="KEGG" id="eai:106833925"/>
    <dbReference type="CTD" id="163589"/>
    <dbReference type="GeneTree" id="ENSGT00940000159902"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
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    <dbReference type="GO" id="GO:0033391">
        <property type="term" value="C:chromatoid body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071546">
        <property type="term" value="C:pi-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030719">
        <property type="term" value="P:P granule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007286">
        <property type="term" value="P:spermatid development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141196">
        <property type="term" value="P:transposable element silencing by piRNA-mediated DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd09985">
        <property type="entry name" value="LOTUS_1_TDRD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09975">
        <property type="entry name" value="LOTUS_2_TDRD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09976">
        <property type="entry name" value="LOTUS_3_TDRD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20419">
        <property type="entry name" value="Tudor_TDRD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000051">
        <property type="entry name" value="Tudor domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.610:FF:000005">
        <property type="entry name" value="Tudor domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.610:FF:000007">
        <property type="entry name" value="Tudor domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.610:FF:000011">
        <property type="entry name" value="tudor domain-containing protein 5 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.610">
        <property type="entry name" value="LOTUS domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041966">
        <property type="entry name" value="LOTUS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025605">
        <property type="entry name" value="OST-HTH/LOTUS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037982">
        <property type="entry name" value="TDRD5_LOTUS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050621">
        <property type="entry name" value="Tudor_domain_containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948">
        <property type="entry name" value="TUDOR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948:SF19">
        <property type="entry name" value="TUDOR DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12872">
        <property type="entry name" value="OST-HTH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0744">Spermatogenesis</keyword>
    <feature type="domain" description="HTH OST-type" evidence="13">
        <location>
            <begin position="7"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="HTH OST-type" evidence="13">
        <location>
            <begin position="127"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="HTH OST-type" evidence="13">
        <location>
            <begin position="296"/>
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    </feature>
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        <location>
            <begin position="538"/>
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            <begin position="947"/>
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            <begin position="883"/>
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            <begin position="961"/>
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    </evidence>
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    </evidence>
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    </evidence>
    <sequence length="1047" mass="117044" checksum="F6899BDBD7B8287E" modified="2023-09-13" version="2">MSEQERMQECLRKEIRSLLISTKDGLTPQQLEKEYLSMVGNHLPLRILGYRSTMELVLDMPDVVSVCPCGDGTVILKAIPDESTKGIASLVAKQRSSHKVRNSVQKGRASVCSGPSSRPRVPYRGRVPPILPAVVKSELKDLLAFSPVLLSDFEKAFAKRFGRSFQYVQYGFLSMFEVLNAASDVISVEQTRGGSLLMLKKSVSEEKQRGWPAGKIFTQPFRVKKQGSYPTGSPVAKTRFSQPTSNVEPPKQVLNVEKASKSNVVETSRLNHTEKLNQLENTFKSVIAQIGPGGTINPELKDKIKFVVSKFPEGLLISKLLGEFEVHFKEQLSPKKLGFLNVTELVGALSDILHVEFREGEQDLLVFDADMKPLPPDGAVSSVRNSCLVQSDKKTEAKACVSSPPRNSLSTAAVKATTWDCPSKNLEEQKQKICKKPNLVVKPLQLQVEISKSQLNLAMANHDIPPDAVRDKKLCRLPPLDTSTLVGVFVEYVISPSQFYIRIYSRDSSEKLEDMMIEMRRCYSNQLVSDRYAMPEYFIQPGHLCCVKISEDKWWYRVIIHRVLGKQEVEVFYPDFGNIGTVQKSALRFLKCCYTKLPAQAIPCSLAWVRPVEEHWTSRAVLQFQKLCGLKPLVGVVDEYVDGILNIFLCDTSSNEDVYFHHVLRLEGHAIVCRENVPSKGFRELNPLALYTESSAGPEDVGLTDLGYLSQQHYFHEDREISPQSKESELYTLQDIKDEKAFSRLKSESKEPKDSEFSSLKTQDKSCEEDPKWCIPRLKDPKEENEDEIPTGMPCLESVTIDDDIWDENWLLLQTKMGNGGDAVSHLFTSGLDGKKPYPSCKEMPPKDWCFSMPKDMWDDSWQPSGLVNGMKVEVQKPEGLGTQENSTDTARSQKQPDLKSSSDSSTLPQLEEFYISLIRSQQSAEGDQVEPNDVQNQEKQIQLSTAALSSAPTVVDSPENHSSSVESSPGSLKNEDFSSSPAIPVFKDKSEGAMGQLSLILSPQHQIPQKLYIPRSTATAALGAAARLATSRSLLHWYPSVKRMDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MFU2</accession>
    <name>A0A8C4MFU2_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ECRG4 augurin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ECRG4</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="J."/>
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                <person name="R."/>
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                <person name="R."/>
                <person name="Yu"/>
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                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the augurin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014699039.1">
        <property type="nucleotide sequence ID" value="XM_014843553.3"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSEAST00005028301.2">
        <property type="protein sequence ID" value="ENSEASP00005026067.1"/>
        <property type="gene ID" value="ENSEASG00005017784.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106832696"/>
    <dbReference type="KEGG" id="eai:106832696"/>
    <dbReference type="CTD" id="84417"/>
    <dbReference type="GeneTree" id="ENSGT00390000000145"/>
    <dbReference type="OMA" id="WLNRGRN"/>
    <dbReference type="OrthoDB" id="8915498at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090398">
        <property type="term" value="P:cellular senescence"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007417">
        <property type="term" value="P:central nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070314">
        <property type="term" value="P:G1 to G0 transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028173">
        <property type="entry name" value="Augurin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31613">
        <property type="entry name" value="AUGURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31613:SF2">
        <property type="entry name" value="AUGURIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15187">
        <property type="entry name" value="Augurin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="43"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011014"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="181" mass="20433" checksum="FED1FC6741D7B4D2" modified="2022-01-19" version="1">MLGAPSRRPLSPAPSPPAQRLTVRAFPSCPLGAAMASSSARPAVLAMTALALLLLLCLGPGGISGNKLKLMLQKREAPAPTKTPVAVDESKAKEFLRSPKRQRRQLWDRSRPEVQQWYQQFLYMGFDEAKFEDDITYWLNRDRNGHDYDDYHQRHYDEDAAIGPRSPDSFRHGASVNYDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MHE4</accession>
    <name>A0A8C4MHE4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">DNA polymerase alpha subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">POLA2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Accessory subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the DNA polymerase alpha subunit B family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014700341.1">
        <property type="nucleotide sequence ID" value="XM_014844855.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MHE4"/>
    <dbReference type="Ensembl" id="ENSEAST00005028955.2">
        <property type="protein sequence ID" value="ENSEASP00005026666.2"/>
        <property type="gene ID" value="ENSEASG00005018030.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106833586"/>
    <dbReference type="KEGG" id="eai:106833586"/>
    <dbReference type="CTD" id="23649"/>
    <dbReference type="GeneTree" id="ENSGT00390000016784"/>
    <dbReference type="OrthoDB" id="336885at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005658">
        <property type="term" value="C:alpha DNA polymerase:primase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.530:FF:000001">
        <property type="entry name" value="DNA polymerase alpha subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.60:FF:000002">
        <property type="entry name" value="DNA polymerase alpha subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.60:FF:000003">
        <property type="entry name" value="DNA polymerase alpha subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.530">
        <property type="entry name" value="DNA polymerase alpha-primase, subunit B, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043034">
        <property type="entry name" value="DNA_pol_alpha_B_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016722">
        <property type="entry name" value="DNA_pol_alpha_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054300">
        <property type="entry name" value="OB_DPOA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013627">
        <property type="entry name" value="Pol_alpha_B_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23061">
        <property type="entry name" value="DNA POLYMERASE 2 ALPHA 70 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23061:SF12">
        <property type="entry name" value="DNA POLYMERASE ALPHA SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22062">
        <property type="entry name" value="OB_DPOA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08418">
        <property type="entry name" value="Pol_alpha_B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018300">
        <property type="entry name" value="DNA_pol_alph_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase alpha subunit B N-terminal" evidence="11">
        <location>
            <begin position="6"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase alpha subunit B OB" evidence="12">
        <location>
            <begin position="217"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="10">
        <location>
            <begin position="342"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="107"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR018300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF08418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026666.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="598" mass="66522" checksum="90E4551B33635447" modified="2023-09-13" version="2">MAVSAQRLLEELQIFGQDCEEELIEKLLELCVYYGQKEEEMVDELIAFCTSTHKANITSETLNSFEHEFLSKRLSKARHSASKDSGHAGARDIVSIQELIEVEEEEETLLNSYTTPSKGSQKRAVITPESSLTKRRVSARSPHQLLSPSSFSPSATPSQKYNSRNNRGEVVTSFGSAQGMSWSGRGGAGNISLKVVGCPESLTGSYKSMFQKLPDIREVLTCRIEELGSELKEHYKIEAFTPVLAPAQEPVILLGQIGCDSNGKLNSKSVILEGDREHSSGAQIPVDLSELKEYSLFPGQVVIMEGINTTGRKLVATKLYEGVPLPFLQPTEEDRDFEQSMVLVACGPYTTSDSITYEPLLDLIAIINRDRPDVCVLFGPFLDAKHEQVESCLLTSPFEDVFKQCLRTIIEGTRSSGSHLVFVPSLRDVHHEPVYPQPPFSYSELPQEDKKRVHFLSEPCSFSINGVIFGLTSTDLLFHMGAEEISSSSGTSDRFSRILKHILTQRSYYPLYPPQEDMAIDYENFYAYAQLPVTPDVFIIPSELRYFVKDILGCVCVNPGRLTKGQVGGTFGRLYLRRQTAEGEGRRSPCVAAQVVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MIV6</accession>
    <name>A0A8C4MIV6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123278018</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MIV6"/>
    <dbReference type="Ensembl" id="ENSEAST00005028698.2">
        <property type="protein sequence ID" value="ENSEASP00005026425.2"/>
        <property type="gene ID" value="ENSEASG00005034029.1"/>
    </dbReference>
    <dbReference type="KEGG" id="eai:106844114"/>
    <dbReference type="GeneTree" id="ENSGT01140000282552"/>
    <dbReference type="OMA" id="MRLMKEV"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15943">
        <property type="entry name" value="7tmA_OR5AP2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="199"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="244"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="279"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="48"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026425.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="317" mass="35737" checksum="9010FAD4E443E352" modified="2023-09-13" version="2">MIGYMKEVQHRNQTEVTEFILLGLSDNSDLQVVLFGLFLLIYMATLVGNLGMIVLIKIDPCLHTPMYLFLSSLSFVDASYSSSVTPKMLMNLVAENKAISFNGCAAQFYFFGSFLGTECFLLAMMAYDRYAAIWNPLLYPVLMSERICFLLIATSFLASFGNAAIHTGMTFRLSFCGSNKINHFYCDTPPLLKLSCSEIHINGLVIMAFSSFNVISCVVIVLISYLCILIAILRMPSLESRHKVFSTCTSHLMAVTIFFGTILFMYLRPTSSYSMEQDKVVSVFYTVVIPMLNPLIYSLKNKDVKWALKKILQKHIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4ML79</accession>
    <name>A0A8C4ML79_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Fucosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">FUT7</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>an N-acetyl-alpha-neuraminyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl derivative + GDP-beta-L-fucose = an alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;4)-[alpha-L-Fuc-(1-&gt;3)]-beta-D-GlcNAc derivative + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56076"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:136545"/>
            <dbReference type="ChEBI" id="CHEBI:139509"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:56077"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Golgi apparatus</location>
            <location evidence="12">Golgi stack membrane</location>
            <topology evidence="12">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyltransferase 10 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4ML79"/>
    <dbReference type="Ensembl" id="ENSEAST00005027714.2">
        <property type="protein sequence ID" value="ENSEASP00005025522.2"/>
        <property type="gene ID" value="ENSEASG00005017390.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005058753.1">
        <property type="protein sequence ID" value="ENSEASP00005036054.1"/>
        <property type="gene ID" value="ENSEASG00005017390.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005078688.1">
        <property type="protein sequence ID" value="ENSEASP00005060317.1"/>
        <property type="gene ID" value="ENSEASG00005017390.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106845523"/>
    <dbReference type="KEGG" id="eai:106845523"/>
    <dbReference type="CTD" id="2529"/>
    <dbReference type="GeneTree" id="ENSGT00940000161618"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017083">
        <property type="term" value="F:4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002361">
        <property type="term" value="P:CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006672">
        <property type="term" value="P:ceramide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007566">
        <property type="term" value="P:embryo implantation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002522">
        <property type="term" value="P:leukocyte migration involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002523">
        <property type="term" value="P:leukocyte migration involved in inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097022">
        <property type="term" value="P:lymphocyte migration into lymph node"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903238">
        <property type="term" value="P:positive regulation of leukocyte tethering or rolling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902624">
        <property type="term" value="P:positive regulation of neutrophil migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060353">
        <property type="term" value="P:regulation of cell adhesion molecule production"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046626">
        <property type="term" value="P:regulation of insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000389">
        <property type="term" value="P:regulation of neutrophil extravasation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001807">
        <property type="term" value="P:regulation of type IV hypersensitivity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072678">
        <property type="term" value="P:T cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11660:FF:000001">
        <property type="entry name" value="alpha-(1,3)-fucosyltransferase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11660">
        <property type="entry name" value="Glycosyl transferase family 10, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055270">
        <property type="entry name" value="Glyco_tran_10_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031481">
        <property type="entry name" value="Glyco_tran_10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001503">
        <property type="entry name" value="Glyco_trans_10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038577">
        <property type="entry name" value="GT10-like_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929">
        <property type="entry name" value="ALPHA- 1,3 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11929:SF12">
        <property type="entry name" value="ALPHA-(1,3)-FUCOSYLTRANSFERASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17039">
        <property type="entry name" value="Glyco_tran_10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00852">
        <property type="entry name" value="Glyco_transf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 12" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase N-terminal" evidence="14">
        <location>
            <begin position="71"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Fucosyltransferase C-terminal" evidence="13">
        <location>
            <begin position="192"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025522.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="367" mass="42270" checksum="53A28B69645ED1E6" modified="2023-09-13" version="2">MAVSVWSLGNPELGGWGLWLSPRRQPLFAWHGPTQRLRALVSLAGVALLMALWLLWLLQSSPGGAPMPQPTLTILIWHWPFTYPPPELPSNTCTSYGVARCHLSTNRSLLATADAVVFHHRELRDGWIRLPLAERPRGQPWVWASMESPSNSHGVSDFRGIFNWVLSYRRDSDIFVPYGRLEPREGPAPPLPPKDRVAAWVVSNFRERQRRVQLYRQLAPHLQVDVFGRANRRPLCASCLLPTVSRYRFYLSFENSQHRDYITEKLWRNALAAGAVPVVLGPPRATYEAFVPPDAFVHVDDFGSARELAAFLTGMNESSYRRYFAWRDRLRVRLVEDWRERFCAICARYPHLPRSQVYQDLQGWFQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MP60</accession>
    <name>A0A8C4MP60_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
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                <person name="S."/>
                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
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                <person name="Wang"/>
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                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MP60"/>
    <dbReference type="Ensembl" id="ENSEAST00005029081.2">
        <property type="protein sequence ID" value="ENSEASP00005026786.2"/>
        <property type="gene ID" value="ENSEASG00005018270.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026786.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="52" mass="5613" checksum="657860B75153F8E2" modified="2023-09-13" version="2">VLAEVGWPQPRVLPGLSAPLSGSLSSPGYTTNLQMFICGIPQRMSLPSSFHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MPH5</accession>
    <name>A0A8C4MPH5_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ST8SIA6</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MPH5"/>
    <dbReference type="SMR" id="A0A8C4MPH5"/>
    <dbReference type="Ensembl" id="ENSEAST00005031666.2">
        <property type="protein sequence ID" value="ENSEASP00005029129.1"/>
        <property type="gene ID" value="ENSEASG00005019834.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106834428"/>
    <dbReference type="KEGG" id="eai:106834428"/>
    <dbReference type="CTD" id="338596"/>
    <dbReference type="GeneTree" id="ENSGT01030000234535"/>
    <dbReference type="OMA" id="NNVEPHR"/>
    <dbReference type="OrthoDB" id="10264956at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003828">
        <property type="term" value="F:alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001835">
        <property type="term" value="P:blastocyst hatching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001574">
        <property type="term" value="P:ganglioside biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd23991">
        <property type="entry name" value="GT29_ST8SIA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000001">
        <property type="entry name" value="ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050943">
        <property type="entry name" value="Glycosyltr_29_Sialyltrsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012163">
        <property type="entry name" value="Sialyl_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11987:SF29">
        <property type="entry name" value="ALPHA-2,8-SIALYLTRANSFERASE 8F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005557">
        <property type="entry name" value="Sialyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034895868" evidence="13">
        <location>
            <begin position="26"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="187"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR005557-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005029129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="399" mass="45228" checksum="A06DBB250E6CA557" modified="2022-01-19" version="1">MRPGGALLALLGSLLLLLLLRLLWCSTDAPARSRLLAEESREATHGTPAALRTLRSPATPLPRARNSTYLNKKSLKLTERCKNLQDGFETLSNKTKKGYSEDDYLRIVTNIQSCPWKRQVEEYENFRAKLASCCDAVQNFIVSQNNTPVGTNMSYEVESKKEILIRERIFNMFPVSQPFTETPYNQCAVVGNGGILNESLCGTEIDESDFVFRCNLPPTTGNVSKDVGSKTNLVTVNPSIIKLKYGNLKEKKAVFLEDIATYGDAFLLLPAFSFRANTGASFKVYDTLKEFKARQKVIFFHPKYLKNLALFWRTKGVTEYRLSSGLMITSVAVELCENVKLYGFWPFSKTVEDTPVSHHYYDNNLPKRGFHEMPKEYRQILQLHVKGILKLQFSKCEIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MRF7</accession>
    <name>A0A8C4MRF7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Zinc finger protein 536</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ZNF536</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Transcriptional repressor that negatively regulates neuron differentiation by repressing retinoic acid-induced gene transcription. Binds and interrupts RARA from binding to retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. Recognizes and binds 2 copies of the core DNA sequence 5'-CCCCCA-3'.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005033483.2">
        <property type="protein sequence ID" value="ENSEASP00005030796.2"/>
        <property type="gene ID" value="ENSEASG00005020978.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156397"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000075">
        <property type="entry name" value="Putative zinc finger protein 536"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000591">
        <property type="entry name" value="Zinc finger protein 536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000615">
        <property type="entry name" value="Zinc finger protein 536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000625">
        <property type="entry name" value="Zinc finger protein 536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051967">
        <property type="entry name" value="Krueppel_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45925">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45925:SF2">
        <property type="entry name" value="ZINC FINGER PROTEIN 536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16606">
        <property type="entry name" value="zf-C2H2_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13909">
        <property type="entry name" value="zf-H2C2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="158"/>
            <end position="180"/>
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    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="274"/>
            <end position="302"/>
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    <feature type="domain" description="C2H2-type" evidence="16">
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            <begin position="345"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="373"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="631"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="751"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="779"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="650"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="802"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="849"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="936"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1129"/>
            <end position="1259"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="675"/>
            <end position="696"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="15">
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            <begin position="855"/>
            <end position="877"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="950"/>
            <end position="961"/>
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            <begin position="1133"/>
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            <begin position="1160"/>
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            <begin position="1191"/>
            <end position="1211"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    <evidence type="ECO:0000256" key="2">
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    <evidence type="ECO:0000256" key="11">
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            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057406"/>
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    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00068635"/>
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    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030796.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1300" mass="141052" checksum="ACC003073606A649" modified="2023-09-13" version="1">MEETSLCLGVSSAAPEAESHLSGPVLNGQYAMSQKLHQITSQLSHAFPELHPRPNPEEKTPAPLEEKAHVPMSGQPMGSQMALLANQLGRDVDTSLNGRVDLQQFLNGQNLGIMSQMSDIEDDARKNRKYPCPLCGKRFRFNSILSLHMRTHTGEKPFKCPYCDHRAAQKGNLKIHLRTHKLGNLGKGRGRVREENRLLHELEERAILRDKQMKSSLLQPRPDLKPLPHAQQAPLATCNLALPANHSVPDVANPVPSPKPASVQEDPVAPAAGFRCTFCKGKFKKREELDRHIRILHKPYKCTLCDFAASQEEELISHVEKAHITAESAQGQGPSSGGEQSANEFRCEVCGQVFSQAWFLKGHMRKHKDSFEHCCQICGRRFKEPWFLKNHMKVHLNKLSVKSKSPSDPELPVPMGGMSQEAHANLYSRYLSCLQSGFMAPDKAGLSEPSQLYSKGELPVKEKEVLGKLLSPISGMAHSVPEGDKHSLLGCLNLVPPLKSSCMERLQAAAKAAEMDPVNSYQAWQLMARGMAMEHGFLSKEHQLQRNHEDTLANAGVLFDKEKREYVLVGADGSKQKMPADLVHSTKVGNPRDLPNKLDPLEGSRDFLSHGLNQALEYNLQGPGNMKEKPTECPDCGRVFRTYHQVVVHSRVHKRDRKGDEDGLQVGLDERRGSGSDQESQSVSRSTTPGSSNVTEESGAGGGLSQTGSAQEDSPHPSSPSSSDIGEEAGRSTGVQQPALLRDRSLGSAMKDCPYCGKTFRTSHHLKVHLRIHTGEKPYKCPHCDYAGTQSASLKYHLERHHRERQNGAGPLSGQPPNPEHRDELSSKTALFIRPDILRGAFKGLPGMDFRGGPASQQWTSGVLPSGDHSGQATGGSSEAPADALKAADLPSKSTHFSEIGRAYQNIVSNGVNFQGSLQAFMDSFVLSSLKKEKDMKDKGLSDPPSTKVHGADGSEEKAGGKAALRKLEKSQYEPLDLSVRPDAASLPGSAVTVQDSVAWHGCLFCAFTTSSLELMALHLQANHLGKAKRKDSVVGVTVNCKDQAREASRMPLLSSVQSSKEMALSHVTAPLDSASEKMAQGQVKETLGEQKSGTWPGHVDPAFCNFPSDFYKQFGVYSGVVGSGVPSSCPNKEPDGKAHLEDDAPILIPETTNKTTTDDLSDIASSEDMDSSKGENNDEEDIETEPEIMSKPLSALSKDSSSDGGDSLLPTGTPQPAQGLVSPLPQVAEKQWHGPGLLPAQDPSVGLPKPERGPQGLEKPMNMLSVLRAYSSDGLAAFNGLASSTANSGCIKRPDLCGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MRJ3</accession>
    <name>A0A8C4MRJ3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Leucine rich repeat transmembrane neuronal 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LRRTM2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Postsynaptic cell membrane</location>
            <topology evidence="11">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the LRRTM family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014709318.1">
        <property type="nucleotide sequence ID" value="XM_014853832.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MRJ3"/>
    <dbReference type="SMR" id="A0A8C4MRJ3"/>
    <dbReference type="Ensembl" id="ENSEAST00005033283.2">
        <property type="protein sequence ID" value="ENSEASP00005030617.1"/>
        <property type="gene ID" value="ENSEASG00005020838.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106839221"/>
    <dbReference type="KEGG" id="eai:106839221"/>
    <dbReference type="CTD" id="26045"/>
    <dbReference type="GeneTree" id="ENSGT00940000160581"/>
    <dbReference type="OMA" id="WPLGARM"/>
    <dbReference type="OrthoDB" id="2325980at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098982">
        <property type="term" value="C:GABA-ergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099634">
        <property type="term" value="C:postsynaptic specialization membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060291">
        <property type="term" value="P:long-term synaptic potentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002091">
        <property type="term" value="P:negative regulation of receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051965">
        <property type="term" value="P:positive regulation of synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050808">
        <property type="term" value="P:synapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000005">
        <property type="entry name" value="leucine-rich repeat transmembrane neuronal protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050467">
        <property type="entry name" value="LRFN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842:SF22">
        <property type="entry name" value="INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN COMPLEX ACID LABILE SUBUNIT ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45842">
        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13855">
        <property type="entry name" value="LRR_8"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00019">
        <property type="entry name" value="LEURICHRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034360856" evidence="14">
        <location>
            <begin position="34"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="423"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="516" mass="59127" checksum="743A5D6D396BDB80" modified="2022-01-19" version="1">MGLHFKWPLGAPMLAAIYAMSMVLKMLPALGMACPPKCRCEKLLFYCDSQGFHSVPNATDQGSLGLSLRHNRITELERDQFASFSQLTWLHLDHNQISTVKEDAFQGLYKLKELILSSNKIFYLPNTTFTQLINLQNLDLSFNQLSSLHPELFYGLRKLQTLHLRSNSLRTIPVRLFWDCRSLEFLDLSTNRLRSLARNGFAGLIKLRELHLEHNQLTKINFAHFLRLSSLHTLFLQWNKISNLTCGMEWTWGTLEKLDLTGNEIKAIDLTVFETMPNLKILLMDNNKLNSLDSKILNSLRSLTTVGLSGNLWECSPRICALASWLGSFQGRWEHSILCHSPDHTQGEDILDAVHGFQLCWNLSTTVTAMTTTYKDPTTEYTKRISSSSYHVGDKEIPTTAGMAVTTEEHFPEPDNAIFTQRVITGTMALLFSFFFIIFIVFISRKCCPPTLRRIRQCSMIQNHRQLRSQTRLHMSNMSDQGPYNEYEPTHEGPFIIINGYGQCKCQQLPYKECEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MSF5</accession>
    <name>A0A8C4MSF5_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">BCR activator of RhoGEF and GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">BCR</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendritic spine</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="ENSEASP00005030330.2"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:Schaffer collateral - CA1 synapse"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:cellular response to lipopolysaccharide"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0060216">
        <property type="term" value="P:definitive hemopoiesis"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0048872">
        <property type="term" value="P:homeostasis of number of cells"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0042472">
        <property type="term" value="P:inner ear morphogenesis"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
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        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:macrophage migration"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:modulation of chemical synaptic transmission"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="active breakpoint cluster region-related protein isoform X1"/>
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    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
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            <begin position="92"/>
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        <location>
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    </feature>
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        <location>
            <begin position="200"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="372"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="453"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030330.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1283" mass="143910" checksum="4934FAD682B3CD91" modified="2023-09-13" version="2">MVDPVGFAEAWKAQFPDSEPPRMELRSVGDIEQELERCKASIRRLEQEVNQERFRMIYLQTLLAKEKKSYDRQRWGFRRAAQPPDGAAEPRAPAPRPLPAPADGADPPPAEEPEVRPDGEGSPGKARPAVARRPGAPAPADRDDRGPPTSVAALRSNFERIRKGHGQTGAAEPEKPFYVNVEFHHERGLVKVNDKEVSDRISSLGSQAMQMERKKSQHGAGPGLGDAPRPSYRGRSSESSSCGLDGDYEDAELNPRFLKDNLINANGGSRPPWPPLEYQPYQSIYVGGMMVEGEGKGPLLRSQSTSEQEKRLTWPRRSCSPRSFEDSGGGYTPDCSSNENLTSSEEDFSSGQSSRVSPSPTTYRMFRDKSRSPSQNSQQSLDSSSPPTPQCQKRHRQGQVVVSEATIVGVRKTGQIWPSDGDSLSGRLPQDGAFHGDTDASFGTSPGYGCAADRAEEQRRHQEGLPYIDDSPSSSPHLSSKGRGSRDALASGALESTKAGELDLEKGLEMRKWVLSGILASEETYLSHLEALLLPMKPLKAAATTSQPVLTSQQIETIFFKVPELYEIHKEFYDGLFPRVQQWSHQQRVGDLFQKLASQLGVYRAFVDNYGVAMETAEKCCQANAQFAEISENLRARSNKDAKDQTTKNSLETLLYKPVDRVTRSTLVLHDLLKHTPCSHPDHPLLQDALRISQNFLSSINEEITPRRQSMTVKKGEHRQLLKDSFMVELVEGARKLRHVFLFTDLLLCTKLKKQSGGKTQQYDCKWYIPLTDLSFQTVDESEAAPNIPLVLDEELEAMKIKISQIKSDIQREKRANKGSKAIERLKKKLSEQESLLLLMSPSMAFRVHSRNGKSYTFLISSDYERAEWRENIREQQKKCFKSFSLTSVELQMLTNSCVKLQTVHCIPLTINKEDDESPGLCGFLNVIVHSATGFKQSSNLYCTLEVDSFGYFVNKAKTRVYRDTAEPNWNEEFEIELEGSQTLRILCYEKCYNKTKIAKEDGETTDRIMGKGQVQLDPQALQDRDWQRTVIAMNGIEVKLSVKFTSREFSLKRMPSRKQTGVFGVKIAVVTKRERSKVPYIVRQCVEEIERRGMEEVGIYRVSGVATDIQALKAAFDVNNKDVSVMMSEMDVNAIAGTLKLYFRELPEPLFTDEFYPNFAEGIALSDPVAKESCMLNLLLSLPEANLLTFLFLLDHLKRVAEKETVNKMSLHNLATVFGPTLLRPSEKESKLPANPSQPISMTDSWSLEVMSQVQVLLYFLQLEAIPAPDSKRQSILFSTEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MV40</accession>
    <name>A0A8C4MV40_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Ferritin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Delivery to lysosomes is mediated by the cargo receptor NCOA4 for autophagic degradation and release of iron.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited. Interacts with NCOA4; NCOA4 promotes targeting of the iron-binding ferritin complex to autolysosomes following starvation or iron depletion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the ferritin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MV40"/>
    <dbReference type="Ensembl" id="ENSEAST00005030237.2">
        <property type="protein sequence ID" value="ENSEASP00005027843.2"/>
        <property type="gene ID" value="ENSEASG00005018932.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182841"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01056">
        <property type="entry name" value="Euk_Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.10:FF:000024">
        <property type="entry name" value="Ferritin heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014034">
        <property type="entry name" value="Ferritin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431:SF37">
        <property type="entry name" value="FERRITIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00540">
        <property type="entry name" value="FERRITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 14 15" id="KW-0408">Iron</keyword>
    <keyword evidence="4 15" id="KW-0409">Iron storage</keyword>
    <keyword evidence="9" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="16">
        <location>
            <begin position="11"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601519-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU361145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005027843.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="133" mass="15641" checksum="68876BE69B0CB586" modified="2023-09-13" version="2">VTTAFPSQVRQNYHQDSEAAINRQINLELHVSCVYLSMSFYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDQDDWENGLKAMECALHLEKNVNESLLELHKLATDKNDPHFNVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MWV3</accession>
    <name>A0A8C4MWV3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Acrosin</fullName>
            <ecNumber evidence="3 14">3.4.21.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ACR</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 14">Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heavy chain (catalytic) and a light chain linked by two disulfide bonds. Forms a heterodimer with SERPINA5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.10" evidence="3 14"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4MWV3"/>
    <dbReference type="Ensembl" id="ENSEAST00005032796.2">
        <property type="protein sequence ID" value="ENSEASP00005030167.2"/>
        <property type="gene ID" value="ENSEASG00005020536.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162430"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007340">
        <property type="term" value="P:acrosome reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000060">
        <property type="entry name" value="Acrosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000002">
        <property type="entry name" value="Transmembrane protease serine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012267">
        <property type="entry name" value="Pept_S1A_acrosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF8">
        <property type="entry name" value="ACROSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001141">
        <property type="entry name" value="Acrosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 14 17" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14 17" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040457826" description="Acrosin" evidence="19">
        <location>
            <begin position="20"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="20">
        <location>
            <begin position="43"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="323"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="380"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="330"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="15">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="15">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="15">
        <location>
            <position position="241"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain (between light and heavy chains)" evidence="16">
        <location>
            <begin position="25"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain (between light and heavy chains)" evidence="16">
        <location>
            <begin position="29"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="74"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="178"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="210"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="237"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR001141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001141-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001141-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030167.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="402" mass="44279" checksum="79D27D07BDB5911D" modified="2023-09-13" version="2">MVEMLPTAVLLVLAVSVVANDNITCDGPCGLRFRQNLQGTLRIIGGQDAALGAWPWMVSLQVFTYHNKRRYHACGGTLLNSHWLVTAAHCFRTKKKAYDWRLIFGAREIQYGSNKPVKPPLQERRVEKIIIHENYSPRSEANDIALLKITPPVPCGHFIGPGCLPQFKAGPPRVPQTCWVAGWGFLKENARKTSPILQEAPVELIDLDLCNSTQWYNGRIRSTNVCAGYPQGKIDTCQGDSGGPLMCRDSMENAYVVVGVTSWGVGCARAKRPGVYTATWPYLNWIASKIGSNALHMIQLGTPPPPTTQALPARLPSIQPIHPLWSFQHPPQPPPPGSPAPKPQPPAPPPPPHQTSTKPPQALSFAKRLQQLIEDLKGKSFSNAKRYYEMETTDLPELTAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N5L0</accession>
    <name>A0A8C4N5L0_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Cholinergic receptor nicotinic alpha 4 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">CHRNA4</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Synaptic cell membrane</location>
            <topology evidence="14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014704619.2">
        <property type="nucleotide sequence ID" value="XM_014849133.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4N5L0"/>
    <dbReference type="Ensembl" id="ENSEAST00005036941.2">
        <property type="protein sequence ID" value="ENSEASP00005033889.2"/>
        <property type="gene ID" value="ENSEASG00005023176.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106836321"/>
    <dbReference type="KEGG" id="eai:106836321"/>
    <dbReference type="CTD" id="1137"/>
    <dbReference type="GeneTree" id="ENSGT00940000159329"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005892">
        <property type="term" value="C:acetylcholine-gated channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034703">
        <property type="term" value="C:cation channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098878">
        <property type="term" value="C:neurotransmitter receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015464">
        <property type="term" value="F:acetylcholine receptor activity"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
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        <text evidence="7">Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G-protein-mediated release of inositol phosphates and activation of MAP kinases.</text>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00070073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005033124.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="192" mass="22413" checksum="521527EA0AFB667E" modified="2023-09-13" version="2">MLRGMYLTRNGNLQRRHTMKEAKDMKNKLGIFRRRNESPGAQPAVKADKVMKSFKPTSEEALKWGESLEKLLLHKYGLAVFQAFLRTEFSEENLEFWLACEDFKKVKSQSKMASKAKKIFAEYIAIQACKEVNLDSYTREHTKDNLQSVTRGCFDLAQKRIFGLMEKDSYPRFLRSDLYLDLINQKKMSPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PF26</accession>
    <name>A0A8C4PF26_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Endoplasmic reticulum membrane sensor NFE2L1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Nuclear factor erythroid 2-related factor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Nuclear factor, erythroid derived 2, like 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">NFE2L1</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type III membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the bZIP family. CNC subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044600273.1">
        <property type="nucleotide sequence ID" value="XM_044744338.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070339191.1">
        <property type="nucleotide sequence ID" value="XM_070483090.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070339193.1">
        <property type="nucleotide sequence ID" value="XM_070483092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PF26"/>
    <dbReference type="Ensembl" id="ENSEAST00005000838.2">
        <property type="protein sequence ID" value="ENSEASP00005000712.1"/>
        <property type="gene ID" value="ENSEASG00005000634.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106826334"/>
    <dbReference type="CTD" id="4779"/>
    <dbReference type="GeneTree" id="ENSGT00950000182892"/>
    <dbReference type="OMA" id="PEGNQEH"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14720">
        <property type="entry name" value="bZIP_NFE2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.880.10:FF:000001">
        <property type="entry name" value="Nuclear factor erythroid 2-related factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.880.10">
        <property type="entry name" value="Transcription factor, Skn-1-like, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004826">
        <property type="entry name" value="bZIP_Maf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047167">
        <property type="entry name" value="NFE2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008917">
        <property type="entry name" value="TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24411:SF31">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MEMBRANE SENSOR NFE2L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24411">
        <property type="entry name" value="NUCLEAR FACTOR ERYTHROID 2-RELATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03131">
        <property type="entry name" value="bZIP_Maf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47454">
        <property type="entry name" value="A DNA-binding domain in eukaryotic transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="25" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="20" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="21" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="19" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="18" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="628"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
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            <begin position="108"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="441"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="564"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="25">
        <location>
            <begin position="646"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="447"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="572"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00030985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00031659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="746" mass="81872" checksum="7EFC0971AE2D23A6" modified="2022-01-19" version="1">MLSLKKYLTEGLLQFTILLSLIGVRVDVDTYLTSQLPPLREIILGPSSAYTQTQFHNLRNTLDGYGIHPKSIDLDNYFTARRLLSQVRALDRFQVPTTEVNAWLVHRDPEGSVSGSQPSSALALESSSGLQDVTGPDNGVRESETEQGFSEDLEDLGAVAPPVSGDLTKEDIDLIDILWRQDIDLGAGREIFDYSHRQKEQDVDKELQDGAEQEDTWPGEGAEALARNLLVDGETGESFPAQFPADISSITEAVPSESEPPGLQNNLLSPLLTGTESPFDLEQQWQDLMSIMEMQAMEVNTSTSEILYNAPPGDPLSTNYSLAPNTPINQNVSLHQASLGGCSQDFSLFSPEVESLPVAGSSTLLPLAPSNSTSLNSTFGSTNLAGLFFPPQLNGTANDTAGPELPDPLGGLLDEAMLDEISLMDLAIEEGFNPVQASQLEEEFDSDSGLSLDSSHSPSSLSSPEGSSSSSSSSSSSSSSSSSASSSASSSFSEEGAVGYSSDSETLDLEEAEGAVGYQPEYSKFCRMSYQDPSQLSCLPYLEHVGHNHTYNMAPSALDSADLPPPSTVKKGSKEKQADFLDKQMSRDEHRARAMKIPFTNDKIINLPVEEFNELLSKYQLSEAQLSLIRDIRRRGKNKMAAQNCRKRKLDTILNLERDVEDLQRDKARLLQEKVEFLRSLRQMKQKVQSLYQEVFGRLRDENGRPYSPSQYALQYAGDGSVLLIPRTLADQQARRQERKPKDRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PGI3</accession>
    <name>A0A8C4PGI3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Actin binding LIM protein family member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ABLIM3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PGI3"/>
    <dbReference type="Ensembl" id="ENSEAST00005004172.2">
        <property type="protein sequence ID" value="ENSEASP00005003803.2"/>
        <property type="gene ID" value="ENSEASG00005002886.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182850"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030032">
        <property type="term" value="P:lamellipodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd09327">
        <property type="entry name" value="LIM1_abLIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09328">
        <property type="entry name" value="LIM2_abLIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09329">
        <property type="entry name" value="LIM3_abLIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09330">
        <property type="entry name" value="LIM4_abLIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000003">
        <property type="entry name" value="actin-binding LIM protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000004">
        <property type="entry name" value="actin-binding LIM protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000007">
        <property type="entry name" value="actin-binding LIM protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000024">
        <property type="entry name" value="actin-binding LIM protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.950.10:FF:000001">
        <property type="entry name" value="actin-binding LIM protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.950.10">
        <property type="entry name" value="Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032402">
        <property type="entry name" value="AbLIM_anchor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051618">
        <property type="entry name" value="Actin-binding_LIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003128">
        <property type="entry name" value="Villin_headpiece"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036886">
        <property type="entry name" value="Villin_headpiece_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24213">
        <property type="entry name" value="ACTIN-BINDING LIM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24213:SF0">
        <property type="entry name" value="ACTIN-BINDING LIM PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16182">
        <property type="entry name" value="AbLIM_anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02209">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00153">
        <property type="entry name" value="VHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47050">
        <property type="entry name" value="VHP, Villin headpiece domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51089">
        <property type="entry name" value="HP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0440">LIM domain</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="21"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="81"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="149"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="HP" evidence="11">
        <location>
            <begin position="615"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="372"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="441"/>
            <end position="475"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="9">
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            <begin position="380"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
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            <begin position="406"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
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            <begin position="454"/>
            <end position="467"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
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        </source>
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        </source>
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            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PH55</accession>
    <name>A0A8C4PH55_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">FERM domain containing kindlin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">FERMT2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the kindlin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070369748.1">
        <property type="nucleotide sequence ID" value="XM_070513647.1"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSEAST00005005687.2">
        <property type="protein sequence ID" value="ENSEASP00005005192.2"/>
        <property type="gene ID" value="ENSEASG00005003881.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106826765"/>
    <dbReference type="CTD" id="10979"/>
    <dbReference type="GeneTree" id="ENSGT00390000013444"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17181">
        <property type="entry name" value="FERM_F0_KIND2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17184">
        <property type="entry name" value="FERM_F1_KIND2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01237">
        <property type="entry name" value="PH_fermitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000037">
        <property type="entry name" value="Fermitin family homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000057">
        <property type="entry name" value="Fermitin family homolog 2 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000035">
        <property type="entry name" value="Fermitin family homolog 2 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037843">
        <property type="entry name" value="Kindlin/fermitin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040790">
        <property type="entry name" value="Kindlin_2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037837">
        <property type="entry name" value="PH_Kindlin/fermitin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16160">
        <property type="entry name" value="FERMITIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16160:SF11">
        <property type="entry name" value="FERMITIN FAMILY HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18124">
        <property type="entry name" value="Kindlin_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="5" id="KW-0965">Cell junction</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="380"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005192.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="680" mass="77790" checksum="46F07D1FE3712ADD" modified="2023-09-13" version="2">MALDGIRMPDGCYADGTWELSVHVTDLSRDVTLRVTGEVHIGGVMLKLVEKLDVKKDWSDHALWWEKKRTWLLKTHWTLDKYGIQADAKLQFTPQHKLLRLQLPNMKYVKVKVNFSDRVFKAVSDICKTFNIRHPEELSLLKKPRDPTKKKKKKLDDQSEDEALELEGPLITPGSGSIYSSPGLYSKTMTPTYDAHDGSPLSPTSAWFGDSALSEGNPGILAVSQPITSPEILAKMFKPQALLDKAKINQGWLDSSRSLMEQDVKENEALLLRFKYYSFFDLNPKYDAIRINQLYEQAKWAILLEEIECTEEEMMMFAALQYHINKLSIMTSENHLNNSDKEVDEVDAALSDLEITLEGGKTSTILGDITSIPELADYIKVFKPKKLTLKGYKQYWCTFKDTSISCYKSKEESSGTPAHQMNLRGCEVTPDVNISGQKFNIKLLIPVADGMNEIWLRCDNEKQYAHWMAACRLASKGKTMADSSYNPEVQNILSFLKMQHLNPDPQFIPEQITADINPECLVSPRYLKKYKNKQITARILEAHQNVAQMSLIEAKMRFIQAWQSLPEFGITHFIARFQGGKKEELIGIAYNRLIRMDAGTGDAIKTWRFSNMKQWNVNWEIKMVTVEFADEVRLSFICTEVDCKVVHEFIGGYIFLSTRAKDQNESLDEEMFYKLTSGWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PHS9</accession>
    <name>A0A8C4PHS9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">dual-specificity kinase</fullName>
            <ecNumber evidence="2">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">DYRK3</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4PHS9"/>
    <dbReference type="Ensembl" id="ENSEAST00005007182.2">
        <property type="protein sequence ID" value="ENSEASP00005006567.2"/>
        <property type="gene ID" value="ENSEASG00005004855.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159878"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902751">
        <property type="term" value="P:positive regulation of cell cycle G2/M phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035617">
        <property type="term" value="P:stress granule disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14224">
        <property type="entry name" value="PKc_DYRK2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000112">
        <property type="entry name" value="Putative dual specificity tyrosine-phosphorylation-regulated kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000127">
        <property type="entry name" value="Putative dual specificity tyrosine-phosphorylation-regulated kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.10.30">
        <property type="entry name" value="DYRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042521">
        <property type="entry name" value="DYRK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF35">
        <property type="entry name" value="DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION-REGULATED KINASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="14">
        <location>
            <begin position="189"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="80"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="218"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006567.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="567" mass="63815" checksum="CF6D68B19FB430D6" modified="2023-09-13" version="2">MKWIKKLGDGVCDTFMMIDETKCPPCSNVLCNPSEPPPPRRLNITAEQLTRDHTQRLLNGGEMKVEQLFQEFGTRRSATFQSDGINDSEKCSPPVSQGKSSDSLNMVKSSSSSKASKAVPLTPEQALKQYKHHLTAYEKLEIINYPEIYFVGPNAKKRHGVIGGPNNGGYDDADGAYIHVPRDHLAYRYEVLKIIGKGSFGQVARVYDHKLRQYVALKMVRNEKRFHRQAAEEIRILEHLKKQDKTGSMNVIHMLESFTFRNHVCMAFELLSIDLYELIKKNKFQGFSIQLVRKFAQSILQSLDALHKNKIIHCDLKPENILLKHHGRSATKVIDFGSSCFEYQKLYTYIQSRFYRAPEIILGSRYSTPIDIWSFGCILAELLTGQPLFPGEDEGDQLACMMELLGMPPAKLLEQSKRVKYFINSKGLPRYCSVTTQADGRVVLVGGRSRRGKKRGPPGSKDWVTALKGCDDYLFIEFLKRCLHWDPSARLTPAQALRHPWISKSAPRPPTIEKVSGKRVVNPTNAFQGLGSKLPPVVGIANKLKANLMSESHGGIPLCSVLPKLMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PIH9</accession>
    <name>A0A8C4PIH9_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Inositol 1,4,5-triphosphate receptor associated 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">IRAG1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PIH9"/>
    <dbReference type="Ensembl" id="ENSEAST00005008829.2">
        <property type="protein sequence ID" value="ENSEASP00005008095.2"/>
        <property type="gene ID" value="ENSEASG00005005874.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106833097"/>
    <dbReference type="CTD" id="10335"/>
    <dbReference type="GeneTree" id="ENSGT00530000063722"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019934">
        <property type="term" value="P:cGMP-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060087">
        <property type="term" value="P:relaxation of vascular associated smooth muscle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008677">
        <property type="entry name" value="MRVI1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15352:SF2">
        <property type="entry name" value="INOSITOL 1,4,5-TRIPHOSPHATE RECEPTOR ASSOCIATED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15352">
        <property type="entry name" value="LYMPHOID-RESTRICTED MEMBRANE PROTEIN, JAW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05781">
        <property type="entry name" value="MRVI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="853"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="35"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="166"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="476"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="705"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="744"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="787"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="68"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="112"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="255"/>
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    </feature>
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        <location>
            <begin position="277"/>
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        </location>
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        <location>
            <begin position="708"/>
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            <begin position="790"/>
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    </feature>
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        <location>
            <begin position="801"/>
            <end position="825"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="911" mass="98417" checksum="B999B376A06A9ADA" modified="2023-09-13" version="2">MVKAPQSEERLARGGTGNNSVLACGAQASRSIFGADAAEVPGTRGHSRQEAAMPHIPEDEEPPGEPQAAQSPAGQGSPTAGISCSPPAIVLTGDATSPEEETDKNLANRAPSPHRRLSHRHLKVSTASLTSVDPAGHVIDLVNDQLPDISISEEDKKKNLALLEEAKSVSERFLTRRGRKSRSSPGDSPAAVSPNLSPGASPVSSRSNSLTVPTPPGLDVCSGPQSPLPGAPPQKGNEAEVSSPLFGEPNVPKGLADRKENDQRKVSQGRLTPRSPTAEKSKEIAIEQKENFDPLQHPGAAPKGPASGTGSGGKMALNSPQPGPAESELGKPLLKAAREGNPLPRSPVPGSGGAAPTAPQGKGTAGEPTGSKVGSKGEPRPPVSRPPLLRGVSWDSGPEEPGPRLQKVLAKLPLAEEEKRFAGKAGSKLAKAPGLKDFQIQVQPVRMQKLTKLREEHILLRNQNLVGLKLPELSEAAEQEKGLPSELSPATEEEETKSGLEVMPNISDMLLRKLRVHKSLPGSAPPLTEKEVENVFVQLSLAFRNDSYTLESRIHQAERERNLTEENTEKELENFKASITSSASLWHHCEHRETYQKLLEDIAVLHRLAARLSSRAEMVGAVRQEKRMSKATEVMMQYVENLKRTYEKDHAELMEFKKLANQNSSRSCGPSEDGVPRTARSMSLTLGKNMPRRRVSVAVVPKFNALNLPGQSPSSSPIPSLPALSESANGKGNLPVTSALPALLENGKTNGDPDCEASAPLPTPSCLEEISQEAKARMEEEAYNKGYQEGLKKTKELQDLKEEEEEQKSESPEEAEEAEETEEEEKDQRSSKLEELVHFLQVLYPKLCQHWRVIWMMAAAMLVLTIVLGLYNSYNSCMEQADGPFGRSTCSAAQRDSWWSSGLQQEQPTEQ</sequence>
</entry>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TMA7 family.</text>
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        <property type="gene ID" value="ENSEASG00005008276.2"/>
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        <property type="entry name" value="TMA7"/>
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        <property type="entry name" value="TRANSLATION MACHINERY-ASSOCIATED PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09072">
        <property type="entry name" value="TMA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006631"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="65" mass="7378" checksum="0FA6A88961C29868" modified="2022-01-19" version="1">MSSTRRPAAKVLMKQPKQQAKEMDEDDKALEQEQKEERKKFEELKATAKQKAPMATAGTKKSGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PQ16</accession>
    <name>A0A8C4PQ16_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zinc finger protein 717</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PQ16"/>
    <dbReference type="Ensembl" id="ENSEAST00005024057.2">
        <property type="protein sequence ID" value="ENSEASP00005022172.2"/>
        <property type="gene ID" value="ENSEASG00005015144.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153505"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000666">
        <property type="entry name" value="RB-associated KRAB zinc finger protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000003">
        <property type="entry name" value="Zinc finger protein 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001157">
        <property type="entry name" value="Zinc finger protein 793"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002196">
        <property type="entry name" value="zinc finger protein 850-like isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050758">
        <property type="entry name" value="Znf_C2H2-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234:SF10">
        <property type="entry name" value="RIKEN CDNA 6720489N17 GENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="13">
        <location>
            <begin position="8"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="261"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="456"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="147"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="148"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="177"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005022172.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="533" mass="60599" checksum="7A9F86B9EAF40A03" modified="2023-09-13" version="2">NVPLQGLVSFEDVAVHFTWEEWQDLDDAQRTLYKDVMLETYSSLASLGHCITKPEVIVKLEQDAEPWTVEAPPNQSLPGGSMRENYGRRLWQAVITNSKTSTKERTDLGKTCNLSSICISKLIINSGNCSGMKPEEFNAFENTFLPRAPDEMPAGEKAEDCPVTGESLRSAEHPSHPQETQTVQQASAFSGRGRGFNKEAVVLTHRARMGGTACRYNGHWQACDESALIAQERRHVGEDRDRCNEWGKTVCEKPTQLKKTFQCNVCGKTFYKKSNLTKHQRTHTGEKPYACDECGKSFYQKPALSVHQRIHRETPHECSGCGKTFHKKSALTAHQRTHTGEKPYECKECGKSFGHRPALTVHQRTHTRDKPYKCNECGKSFCVKPKLTVHLRLHTGEKPYECEECGKTFYQKSKLTVHQRTHTGEKPYKCNECRKTFCEKSTLNRHQRTHTGEKPYGCKECRKTFYQKSALTVHQRTHTGEKPYECNECGKSFCQKSHLSKHLRTHTGTNLPHQKKKKCIYASMQGPASWHSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PQ78</accession>
    <name>A0A8C4PQ78_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">A-kinase anchoring protein 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">AKAP13</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005024468.2">
        <property type="protein sequence ID" value="ENSEASP00005022549.2"/>
        <property type="gene ID" value="ENSEASG00005015400.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154146"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005078">
        <property type="term" value="F:MAP-kinase scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071875">
        <property type="term" value="P:adrenergic receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071356">
        <property type="term" value="P:cellular response to tumor necrosis factor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="C1_AKAP13"/>
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        <property type="entry name" value="RhoGEF"/>
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        <property type="entry name" value="Rho guanine nucleotide exchange factor 2"/>
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        <property type="entry name" value="Rho guanine nucleotide exchange factor 2"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="entry name" value="C1-like_sf"/>
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        <property type="entry name" value="DBL_dom_sf"/>
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        <property type="entry name" value="PH-like_dom_sf"/>
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        <property type="entry name" value="Rho_GEF"/>
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    <dbReference type="PANTHER" id="PTHR13944:SF18">
        <property type="entry name" value="A-KINASE ANCHOR PROTEIN 13"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AGAP007712-PA"/>
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        <property type="entry name" value="DBL homology domain (DH-domain)"/>
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        <property type="entry name" value="ZF_DAG_PE_2"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="13">
        <location>
            <begin position="1836"/>
            <end position="1883"/>
        </location>
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    <feature type="domain" description="DH" evidence="12">
        <location>
            <begin position="2037"/>
            <end position="2234"/>
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            <begin position="2274"/>
            <end position="2376"/>
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            <begin position="303"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
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            <begin position="376"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
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            <begin position="556"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
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            <begin position="629"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="781"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="918"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1184"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1253"/>
            <end position="1281"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1448"/>
            <end position="1525"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1615"/>
            <end position="1661"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
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            <begin position="1739"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
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            <begin position="1800"/>
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            <begin position="2489"/>
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            <begin position="2390"/>
            <end position="2421"/>
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            <begin position="303"/>
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            <begin position="562"/>
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            <begin position="640"/>
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            <begin position="2802"/>
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    </evidence>
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            <dbReference type="SAM" id="Coils"/>
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    <evidence type="ECO:0000256" key="10">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="11">
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            <dbReference type="PROSITE" id="PS50003"/>
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            <dbReference type="PROSITE" id="PS50081"/>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005022549.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="2857" mass="310280" checksum="293A309F6AC222A8" modified="2023-09-13" version="2">MKLNPQQAPLYGDCVVTVLLAEEDKVEDDVVFYLVFSGSTLHHCTSTRKVSSDTLETIAPGHDCCETVKVLLCASKEGLPVFVVAEEDFQFIQDEAYDAAQFLATSAGNQQALNFTRFLDRSGPPSTDVNSLDEKVALAFRHLKLPAEWNVLGTDQTLHDGGPRETLMHFAVRLGLLRLTWFLLQKPGGRGALSIHNQEGATPVSLALERGYHQLHQLLTEENAGEPDSWSSLSYEIPYGDCSVRHHRELDIYTLTSESESHHEPPVPADSCTGHIFKLMNIQQQLMKTNLKQMDNLVPLMVTTQDPSSLPSAQDTDGQFLPCASEPTDNQRLSSPEDTESSSCCQASTVGQVESSCDLSRVVEEENTDCSCRKENKGVQRRGDEAEPTPAVDSGTVSDPDSCLQSMPECGGKGTEGLPSCGIRNEETGTKSAVAAAQESPSSGDRVLQGDVVHPGTAQCFSAGEPAVSSTADASVPETAGERERGLMNPDATVQKNVLEGGESTKERLENSPVSTAGASDVRVTSKPVDKASVANCVSATGSLDGNKPAESLFAFSNGETSTEKTAETEASRSCEESADDPVGQNLPVIPAAAKDKISDGFQPDTPLASLCEAVSPSHLTVCRPQKDVMPNQKSETHSSHVQSQNSQSPICATTGDDKLCVASSCPQDTVTSTGVLAAKRGDGVVTQPESTTTGLPNTHLPPAICSEGPQANTATPDPVRDTQKPVDFCPLEVWDKEDQGKDLKLETSLTNMLEVALHPHAVVPKAEKELVPDQAVTSDRTFSLAGSPGSESVTKDDALSLGPSQKEKGTATPELHTATDGRDGSDGDSNDPDKQSLEDSAAGLSPSCTAPDLQPTMGNTSPIGLGGEHEGPCLSAAPEVLNIEEGTDSSLLHVSKATSASDSILTEEGKSLVVLEGSAAQGQGDKDKAVACSSIKEDALSSDTLQEEHRTPPPGQEAPGSRGEPSSAVCAEDRALQQGNSLGTPSACLHAEAKHNKEVAPPVSLLTEGGAAQSLVPPGASLADTNQEALGAEQSSSPLLPGLLPHGSQALNCSRSVGVKNTQSQGKNTACEVSGNVALDVAVVNAPQDTPGARRKDVSHNTQDIPIPKVSLSQEKNMILGLQGAVSDKGVTDLPGAATPEMVPLGWEKGKQEGADSSCGVGDSKEARMDGEARHVLLQPIAKELPKETGLSSSDDKAPSQERGASPLPCTVAAEATHLPQRANSIEEAASRIVDAVIEQVRASGALVTEGEISHMSLSSPSETGPETEQLESASPGKVSALLPGETPQMGSIRGEAPGNRAGCFAGREEPEEMILPVRGPKPATEMPDTKAEDEVDFLSNTRESSVSEEVATGNITAAPKMKQGPKTQAINRESWCAIEPCPDAASLLASARSPECENFLDVGLGRECASKQGVLKRQSGSDSDLFHSPSEEVDSIIFSKPEEEQLGCDITGSSSSTDDTASLDRHSSHGSDISLPQISHLSRSRDQQCLDGSHGHGVGAEGRESESEPAGSGEMEEEEMDSITEVPANCSVLRSSMRSLSPFRRHSWGPGKNAASDAEMNHRSSMRVLGDVVRRPPIHRRSMSWCPSGVQYSAALSPDFNYRSFSLEGLTGGAGVGNKPASSLEVSSADTRELRHPFSGEERGDSLMSLSEEDLESGQREHRVFDQQACHRSKKQGFNYCTSAISSPLTKSVSLMTISHPGLDTQQQMQPARRISFSVSISPLLLKSKTLFSLGSSYSSDEDEELRNSRPFHSTSANLTESITEENYGFLPPSPSKKDFERTRGTKVSRTFSYIKNKMSSSKKSKEKEKEKDKIKEKEKDSKEKEKDKKALNGHAFSSIPVVGPISCSQCLKPFTNKDAYTCATCGVFVHKGCRESLASCAKVRMKQPKGSLQAHDTSSLPTVIMRNKSSQPKERPRSAVLLADETTAAPMFTNRRSQQSVSLSKSVSIQNIAGVGHDENISNTWKFLSHSTDSLNKISKVNESTESLTDEGTDMNEGQLMGDFEIESKQLEAESWSRIVDSKFLKQQKKDVVKRQEVIYELMQTELHHVRTLKIMSDVYSRGMMTDLLFEQQMVEKLFPCLDELISIHSQFFQRILERKKESLVDKSEKNFLIKRMGDVLVNQFSGENAERLKKTYGKFCGHHNQSVNYFKDLYTKDKRFQAFVKKKMSSSVVRRLGIPECILLVTQRITKYPVLFQRILQCTKDNEVEQEDLAQSLSLVKDVIGAVDSKVASYEKKVRLNEIYTKTDSKSIMRMKSGQMFAKEDLKRKKLVRDGGVFLKSTTGRLKEVQAVLLTDILVFLQEKDQKYIFASLDQKSTVISLKKLIVREVAHEEKGLFLISMGMKDPEMVEVHASSKEERNSWIQIIQDTINTLNRDEDEGIPSENEEEKKLLDTKARELKEQLQQKDQQILLLLEEKEMIFRDMTECSTPLPDDCSPTHSSRTLFRSNTEEALKGGPLMKSAINEVEILQGLVSGSLGGTLGQAVSSPVEQEGTVGPVSLPRRAETFGGFDSHQMNASKGGEKEEGDDGQDLRRTESDSGLKKGGNANLVFMLKRNNEQVVQSVVHLHELLSSLQGVVLQQDSYIEDQKLMLSERALARSLSRPSSLVEQEKQRSLEKQRQDLANLQRQQAQHAEERRRREREWEACERALQEREARLAQREEALQRGQRDLERERAELQQRKGAYQGDLERLRAAQRQLEREQEQLRRDAERPRLPERDVGQVSHQHSKLLRIPSFLPNPEECPLPSAPSIAKSGSLDSELSVSPKRNSISRTPKDKGPFHILSSTSQTSKVPEGPSQTPAASSSCSSTRLFGLAKPKDKKDKKKKSKGGRSQPGDGPASEGPVEGEEIFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PRH6</accession>
    <name>A0A8C4PRH6_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Desmoglein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">DSG2</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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    <keyword evidence="4" id="KW-0165">Cleavage on pair of basic residues</keyword>
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    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="21"/>
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    <sequence length="1107" mass="121361" checksum="6B867FDE75AF9F87" modified="2023-09-13" version="2">MARRAEACLLLVLICCNFGNGLHLEVFNTRNENELLSKHTHLVRQKRAWITAPVALREGEDLSKKNPIAKIHSDIAEEKGLKITYKYTGKGITEPPFGVFVFNKDTGDLNITRILDREETPYFLLMGYALDVRGNNLEKPLELRIKVLDINDNEPVFTQAVFAGSIEELSAAHTLVMTVIATDADEPNTMNSKISYRIVSQEPASSPVFHLNKDTGEIYTTSVALDREEHSSYTLTVEARDGNGQITDKPVTQAQVQIRILDVNDNIPVAEKETYEGTVEENKANVEVIRIKVFDADEIGSDNWLANFTFASGNEKGYFHIETDTQTNEGIVTLIKEVDYEEIKNLNFSIIVTNKAAFHKSVKNKYKPISIPITVKVKNVKEGIHFKSSTISIRTSESMDKASLSRIIGKFEAFDEDTGKLAQVRYAKFEDIDNWISVNSVTSEIRLVKIPDFESRYVHNGTYTAKIVAISEDYPRKTITGTIVITVEDINDNCPTLINPVQTVCDDVQHVNVTAQDLDGYPYSWPFSFSIIDTPAGMAERWKIVHQESTSVLLQQSERKLGRSEIQFLISDRQGFTCPEKQTLDLTVCNCLDGGGCVEALHDSYVGLGPAAIALIIFALLLLLLVPLLLLMCHCGEGAKGFTPIPGTIEMLHPWNNEGAPPEDKVVSSFVAADHGVHVSNGVGGVTTKTITKGSSSSSYTKGHEVDGRWKERRRLISVDPPQVTGLTRVIDSETITTTRTIGASRDMTGAEAAAVALSDEFLRNYFTEKAASYTEEDDVHLGKDCLLVYSQEETESLHGSIGCCSFIEGELDDGFLDDLGIKFKTLAEVCVGRKINIDMQIEQKPKPVRETGVKTASHSLYEQTKVNSENASSSGSSFQVPEHLHEANAEKVTQEVVTERSVSSRQGQKVPRPLPDPLPPGNVILKETSYATGSTMPPSTMILGPSHPQGLIVTERVYAPASTLVDQHYASEGNVTVTERIIQPNGGVSGPLEGTQQLPDAHYVMVRERESFLAPSPAVQPTLTMPSVAVGQNVTVTERVLTPASALQSGFQIPAETSVTARKTVVSGAGVTGPPPNFSLEESGHSHSTVTTSSTRVSKHSTVQHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PRK2</accession>
    <name>A0A8C4PRK2_EQUAS</name>
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        <submittedName>
            <fullName evidence="8">Formin 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">FMN1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the formin homology family. Cappuccino subfamily.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005026906.2">
        <property type="protein sequence ID" value="ENSEASP00005024795.2"/>
        <property type="gene ID" value="ENSEASG00005016817.2"/>
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<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I5U7</accession>
    <name>A0A9L0I5U7_EQUAS</name>
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        <recommendedName>
            <fullName evidence="8">Ubiquitin-conjugating enzyme E2 U</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">E2 ubiquitin-conjugating enzyme U</fullName>
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        <alternativeName>
            <fullName evidence="11">Ubiquitin carrier protein U</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ubiquitin-protein ligase U</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">UBE2U</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the covalent attachment of ubiquitin to other proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSEASP00005035532.1"/>
        <property type="gene ID" value="ENSEASG00005008168.2"/>
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        <property type="component" value="Chromosome 16"/>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="UBCc_UBE2U"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ubiquitin-conjugating enzyme E2 U isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ub_conjugating_enzyme"/>
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        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="14">
        <location>
            <begin position="37"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="339"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="364"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="12">
        <location>
            <position position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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            <dbReference type="ARBA" id="ARBA00022786"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00043952"/>
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            <dbReference type="ARBA" id="ARBA00076315"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00077510"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00082135"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="413" mass="47555" checksum="CEA0B33FB325A116" modified="2023-09-13" version="1">MSVAWVWRVTPKPQTPASESPRGAWGQILCCLIMHCRAYFLLEKDFLELKENNYPGIAAFPVSEDLLEWEAEIEGLQNTIWHGLPFQLTINFTPEYNFVPPTVKFRTIPFHPNVDLYTGRPCIDFLDNPDKWSTSYTLSSILLTLQVMLSNPVLENPVNLEAAQILIKNEHMYSLIVLRLFDQPVQYLLPVAPSAHPPSVWTGVLSWYPGEPPACLWAWSSCSLPRLPLGWFAPSFGWSTSRSFLVVKDDYPELRKDPDKFIRSIKVVSFDDYYKTWSGIATSKPTDYYRTPLFEDQNFIAKYYKWKKMELKHPKEWSLKYAAARARLARENRGPYRANYPTQRSHLCPTPVQLLPDSQTETDSLTKSDETEEGWKNEALLDANNTNESWEDEVEDLVAWTNTLDANALEDSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I9I6</accession>
    <name>A0A9L0I9I6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="12">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">FKBP6</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the FKBP6 family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="12"/>
    <dbReference type="RefSeq" id="XP_070341494.1">
        <property type="nucleotide sequence ID" value="XM_070485393.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0I9I6"/>
    <dbReference type="Ensembl" id="ENSEAST00005043396.1">
        <property type="protein sequence ID" value="ENSEASP00005036736.1"/>
        <property type="gene ID" value="ENSEASG00005016112.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837095"/>
    <dbReference type="KEGG" id="eai:106837095"/>
    <dbReference type="CTD" id="8468"/>
    <dbReference type="GeneTree" id="ENSGT00940000158514"/>
    <dbReference type="OrthoDB" id="8116123at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045171">
        <property type="term" value="C:intercellular bridge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000795">
        <property type="term" value="C:synaptonemal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034587">
        <property type="term" value="P:piRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045070">
        <property type="term" value="P:positive regulation of viral genome replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141196">
        <property type="term" value="P:transposable element silencing by piRNA-mediated DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000160">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.40:FF:000030">
        <property type="entry name" value="Peptidylprolyl isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042282">
        <property type="entry name" value="FKBP6/shu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46674">
        <property type="entry name" value="INACTIVE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46674:SF1">
        <property type="entry name" value="INACTIVE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE FKBP6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0469">Meiosis</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="12" id="KW-0697">Rotamase</keyword>
    <keyword evidence="8" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="7" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="13">
        <location>
            <begin position="43"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005036736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="316" mass="36013" checksum="8E12EA28277855DC" modified="2023-09-13" version="1">MGGSAPGQSPYQRLSQRMLDISGDRGVLKDVIREGAGELVTPDASVLVKYSGYLEHMDKPFDSNCFRKTPRLMKLGEDITLWGMELGLLSMRRGELARFLFKPTYAYGTLGCPPLIPPNTTVLFEIELLDFLDSAESDKFCALSAEQQDQFPLQKVLKVAATEREFGNYLFRQNRFYDAKVRYKRALLLLHRRSAPPEEQHLVEAAKLLVLLNLSFTYLKLERPTTALRYGEQALVIDQKNAKALFRCGQACLLMTEYQKARDFLVQAQKAQPFNHDINNELKKLASCYKDYTDKEKEMCHRMFAPCDDDSAVGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I9P4</accession>
    <name>A0A9L0I9P4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Golgi phosphoprotein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">GOLPH3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">Golgi stack membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GOLPH3/VPS74 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0I9P4"/>
    <dbReference type="Ensembl" id="ENSEAST00005067722.1">
        <property type="protein sequence ID" value="ENSEASP00005037449.1"/>
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        <property type="entry name" value="Golgi phosphoprotein 3"/>
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        <property type="entry name" value="yeast vps74-n-term truncation variant domain like"/>
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        <property type="entry name" value="GPP34-like"/>
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        <property type="entry name" value="GOLGI PHOSPHOPROTEIN 3"/>
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        <property type="entry name" value="TRANS-GOLGI PROTEIN GMX33"/>
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        <property type="entry name" value="GPP34"/>
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    <keyword evidence="4" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
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        <location>
            <begin position="1"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="14"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004344"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004546"/>
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            <dbReference type="ARBA" id="ARBA00007284"/>
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        <source>
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    <sequence length="288" mass="32544" checksum="DADC33ADBB4FBF61" modified="2023-09-13" version="1">MTSLTQRSSGLVQRRTEASRSAADKERAAGGGGGGGEDDAQSRRDEQDDDDKGDSKETRLTLMEEGYTSFWNDCISSGLRGCMLIELALRGRLQLEACGMRRKSLLTRKVICKSDAPTGDVLLDEALKHVKETQPPETVQNWIELLSGETWNPLKLHYQLRNVRERLAKNLVEKGVLTTEKQNFLLFDMTTHPLTNNNIKQRLIKKVQEAVLDKWVNDPHRMDKRLLALIYLAHASDVLENAFAPLLDEQYDLATKRVRQLLDLDPEVECLKANTNEVLWAVVAAFTK</sequence>
</entry>
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    <accession>A0A9L0I9X0</accession>
    <name>A0A9L0I9X0_EQUAS</name>
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        <recommendedName>
            <fullName evidence="8">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
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                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Guo"/>
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                <person name="Yang"/>
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                <person name="Zhuang"/>
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                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the serpin family.</text>
    </comment>
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        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:extracellular space"/>
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        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
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        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
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        <property type="entry name" value="Serpin_fam"/>
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        <property type="entry name" value="Serpin_sf"/>
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    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF165">
        <property type="entry name" value="ALPHA-1-ANTITRYPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
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        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Serpin" evidence="8">
        <location>
            <begin position="47"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="392"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
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        </source>
    </evidence>
    <sequence length="423" mass="46386" checksum="DE0AA5FF0044E804" modified="2023-09-13" version="1">MPSSITWCLLLLAGLCCLVSGSLYTSSHDEQPPPLHSLGRKLSNFAFSLYRVLAAQSNTTNILFPQLSITTTIVLISLGAKGDTHTQILQTLDFNCKDTFEIYIGLPHFVNRLHQPRQSLLMSTDNILVIDKNLKKVGTFLVDARELFHPEEQISEYAQKTTGRKLVDLINDLDKNTALAPVNYIFFHGKGRDDCDAGRSIKADFQVDENATITVPMVKRIGDFFLHWTFELSSFVLLQRYTGSLFTFLIWEKGSERKHFQCSETPLFSNLRDSSANIHVLQLSLSGTYHLKTILGKLGITKAFSLEADLSGITEEVPLTVSKVSLSLYWTGSEGRAEEAASALLVSQGPPPGQTPRPLCHLCAGPSLLGLDGPVFTKTGLGSGLSEHFGPLSPLTLGDPGESLSPSEPGTSAEMPFLQENCR</sequence>
</entry>
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    <accession>A0A9L0ID00</accession>
    <name>A0A9L0ID00_EQUAS</name>
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        <recommendedName>
            <fullName evidence="27">Peroxisomal multifunctional enzyme type 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="29">17-beta-hydroxysteroid dehydrogenase 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">D-bifunctional protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">Multifunctional protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">HSD17B4</name>
    </gene>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="26">Bifunctional enzyme acting on the peroxisomal fatty acid beta-oxidation pathway. Catalyzes two of the four reactions in fatty acid degradation: hydration of 2-enoyl-CoA (trans-2-enoyl-CoA) to produce (3R)-3-hydroxyacyl-CoA, and dehydrogenation of (3R)-3-hydroxyacyl-CoA to produce 3-ketoacyl-CoA (3-oxoacyl-CoA), which is further metabolized by SCPx. Can use straight-chain and branched-chain fatty acids, as well as bile acid intermediates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oyl-CoA + NAD(+) = 3alpha,7alpha,12alpha-trihydroxy-24-oxo-5beta-cholestan-26-oyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:47088"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58507"/>
            <dbReference type="ChEBI" id="CHEBI:59807"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:47089"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oyl-CoA = (24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-en-26-oyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:18933"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:59807"/>
            <dbReference type="ChEBI" id="CHEBI:59879"/>
            <dbReference type="EC" id="4.2.1.107"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:18935"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(2E)-hexadecenedioyl-CoA + H2O = (3R)-hydroxyhexadecanedioyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:40255"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:77075"/>
            <dbReference type="ChEBI" id="CHEBI:77079"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:40256"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>(2E)-octenoyl-CoA + H2O = (3R)-hydroxyoctanoyl-CoA</text>
            <dbReference type="Rhea" id="RHEA:40187"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:62242"/>
            <dbReference type="ChEBI" id="CHEBI:74279"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:40188"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>(3R)-3-hydroxydecanoyl-CoA + NAD(+) = 3-oxodecanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45832"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62548"/>
            <dbReference type="ChEBI" id="CHEBI:74272"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:45833"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(3R)-3-hydroxydecanoyl-CoA = (2E)-decenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:45992"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:61406"/>
            <dbReference type="ChEBI" id="CHEBI:74272"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:45994"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(3R)-hydroxyhexadecanedioyl-CoA + NAD(+) = 3-oxohexadecanedioyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40263"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:77079"/>
            <dbReference type="ChEBI" id="CHEBI:77081"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:40264"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(3R)-hydroxyhexadecanoyl-CoA + NAD(+) = 3-oxohexadecanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40243"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57349"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:74278"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:40244"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(3R)-hydroxyhexadecanoyl-CoA = (2E)-hexadecenoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:39159"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
            <dbReference type="ChEBI" id="CHEBI:74278"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="22">
            <dbReference type="Rhea" id="RHEA:39161"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(3R)-hydroxyoctanoyl-CoA + NAD(+) = 3-oxooctanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40191"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62619"/>
            <dbReference type="ChEBI" id="CHEBI:74279"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:40192"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>a (3R)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32711"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57319"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="1.1.1.n12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:32712"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a (3R)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O</text>
            <dbReference type="Rhea" id="RHEA:26526"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57319"/>
            <dbReference type="ChEBI" id="CHEBI:58856"/>
            <dbReference type="EC" id="4.2.1.119"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="23">
            <dbReference type="Rhea" id="RHEA:26528"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014699560.1">
        <property type="nucleotide sequence ID" value="XM_014844074.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0ID00"/>
    <dbReference type="Ensembl" id="ENSEAST00005051348.1">
        <property type="protein sequence ID" value="ENSEASP00005038794.1"/>
        <property type="gene ID" value="ENSEASG00005014918.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106833039"/>
    <dbReference type="KEGG" id="eai:106833039"/>
    <dbReference type="CTD" id="3295"/>
    <dbReference type="GeneTree" id="ENSGT00940000158343"/>
    <dbReference type="OMA" id="GKTRWQR"/>
    <dbReference type="OrthoDB" id="3592703at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018812">
        <property type="term" value="F:3-hydroxyacyl-CoA dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033989">
        <property type="term" value="F:3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016616">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03448">
        <property type="entry name" value="HDE_HSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05353">
        <property type="entry name" value="hydroxyacyl-CoA-like_DH_SDR_c-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1050.10:FF:000004">
        <property type="entry name" value="Hydroxysteroid 17-beta dehydrogenase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4290:FF:000001">
        <property type="entry name" value="Peroxisomal multifunctional enzyme type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000013">
        <property type="entry name" value="Peroxisomal multifunctional enzyme type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.129.10:FF:000019">
        <property type="entry name" value="peroxisomal multifunctional enzyme type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000185">
        <property type="entry name" value="peroxisomal multifunctional enzyme type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1050.10">
        <property type="entry name" value="SCP2 sterol-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057326">
        <property type="entry name" value="KR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002539">
        <property type="entry name" value="MaoC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054357">
        <property type="entry name" value="MFE-2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051687">
        <property type="entry name" value="Peroxisomal_Beta-Oxidation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003033">
        <property type="entry name" value="SCP2_sterol-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036527">
        <property type="entry name" value="SCP2_sterol-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024">
        <property type="entry name" value="DEHYDROGENASES, SHORT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45024:SF2">
        <property type="entry name" value="SCP2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01575">
        <property type="entry name" value="MaoC_dehydratas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22622">
        <property type="entry name" value="MFE-2_hydrat-2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02036">
        <property type="entry name" value="SCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00822">
        <property type="entry name" value="PKS_KR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55718">
        <property type="entry name" value="SCP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ketoreductase" evidence="31">
        <location>
            <begin position="10"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00050743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00051981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00052006"/>
        </source>
    </evidence>
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</entry>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
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                <person name="Fang"/>
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                <person name="Zhao"/>
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                <person name="Wang"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Gao"/>
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                <person name="G."/>
                <person name="Zhang"/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
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                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Nucleus</location>
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        <text evidence="3">Belongs to the DACH/dachshund family.</text>
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        <property type="entry name" value="DNA-bd_dom_put_sf"/>
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    <dbReference type="InterPro" id="IPR037000">
        <property type="entry name" value="Ski_DNA-bd_sf"/>
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            <begin position="20"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IGA8</accession>
    <name>A0A9L0IGA8_EQUAS</name>
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        <submittedName>
            <fullName evidence="5">Coiled-coil domain containing 166</fullName>
        </submittedName>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Sun"/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Yu"/>
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                <person name="Yin"/>
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                <person name="Liu"/>
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        <location>
            <begin position="294"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF14988"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005036073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="398" mass="45192" checksum="C1886FC14E84720A" modified="2023-09-13" version="1">MAPKRRRGPSTGRRPGAAKEGAEAPLSERAQYLQREYKLLSEQLDACEQRVDQVLRENSFLDREAQRLREENRLYTSYVSARAQRCAKAIVRLDEQNRVDLTQVHWERGELASHYHGREDGVRAQLLEMEARAAQMARQVQELQPYKELQLEQLARIRALERELLHMRVEHTQLLHRVKRRFTEDKAAFEREARQRVQSLARRAEREAARALLAHTQAIKADNARLRQELLRLLQRAQLLHDTRRQLLEQREKLRREHEDTRDLARVHGWLRRGPGGPPLWQPLPVASRPASFASPTGPSRAASRSPSNAASRVPSLLSSRMASGVPSLISSRVGSRVPSLPLSKASSRVPSLTLSRLASRVSSRSSLRAASQNILLSAKPVLGGGSSYPRVEGHHEH</sequence>
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            <fullName evidence="2">Mitochondrial inner membrane protease ATP23 homolog</fullName>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Liu"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
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                <person name="Li"/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
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                <person name="Pan"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="8" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the peptidase M76 family.</text>
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        <property type="project" value="GOC"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:mitochondrial protein processing"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Peptidase_M76_ATP23"/>
    </dbReference>
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        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21711:SF0">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE ATP23 HOMOLOG"/>
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    </dbReference>
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        <property type="entry name" value="Peptidase_M76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="38"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
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        <location>
            <begin position="114"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009915"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00016555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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            <taxon>Equus</taxon>
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    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="X."/>
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                <person name="S."/>
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                <person name="Y."/>
                <person name="Liu"/>
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                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Xu"/>
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                <person name="Liu"/>
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                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
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                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Golgi apparatus membrane</location>
            <topology evidence="1 11">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the sulfotransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0II02"/>
    <dbReference type="Ensembl" id="ENSEAST00005050430.1">
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160563"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
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    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008146">
        <property type="term" value="F:sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030166">
        <property type="term" value="P:proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018011">
        <property type="entry name" value="Carb_sulfotrans_8-10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005331">
        <property type="entry name" value="Sulfotransferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12137:SF6">
        <property type="entry name" value="CARBOHYDRATE SULFOTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Wang"/>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
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                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
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                <person name="J."/>
                <person name="Pan"/>
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                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <text evidence="11">Ferric-chelate reductases reduce Fe(3+) to Fe(2+) before its transport from the endosome to the cytoplasm.</text>
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    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
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    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="2">Multi-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="3">Belongs to the FRRS1 family.</text>
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        <property type="gene ID" value="ENSEASG00005015739.2"/>
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        <property type="entry name" value="Reeler_sf"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
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    <feature type="chain" id="PRO_5040126185" description="Ferric-chelate reductase 1" evidence="14">
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    </evidence>
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            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0ILC4</accession>
    <name>A0A9L0ILC4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Shisa family member 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SHISA9</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Regulator of short-term neuronal synaptic plasticity in the dentate gyrus. Associates with AMPA receptors (ionotropic glutamate receptors) in synaptic spines and promotes AMPA receptor desensitization at excitatory synapses.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell projection</location>
            <location evidence="10">Dendritic spine membrane</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the shisa family. SHISA9 subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSEASP00005041750.1"/>
        <property type="gene ID" value="ENSEASG00005029432.1"/>
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    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
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        <property type="term" value="C:AMPA glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0032591">
        <property type="term" value="C:dendritic spine membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:postsynaptic density"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:postsynaptic membrane"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:regulation of short-term neuronal synaptic plasticity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Shisa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053891">
        <property type="entry name" value="Shisa_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31774:SF1">
        <property type="entry name" value="PROTEIN SHISA-9"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR31774">
        <property type="entry name" value="PROTEIN SHISA-9-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF13908">
        <property type="entry name" value="Shisa_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0770">Synapse</keyword>
    <keyword evidence="2 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="193"/>
            <end position="216"/>
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    <feature type="domain" description="Shisa N-terminal" evidence="15">
        <location>
            <begin position="113"/>
            <end position="163"/>
        </location>
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        <location>
            <begin position="1"/>
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        <location>
            <begin position="358"/>
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        <location>
            <begin position="418"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="439"/>
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        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00029429"/>
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            <dbReference type="ARBA" id="ARBA00038448"/>
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            <dbReference type="SAM" id="Phobius"/>
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            <dbReference type="Pfam" id="PF13908"/>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005041750.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="449" mass="49019" checksum="B6FA5CA70BC7F067" modified="2023-09-13" version="1">MCGPRRLAAPGSSLGSFGRASRGGCSGSSGGRAAEPGLGDTMRRVLRLLLGCFLTELCARVCRAQERAGHGQLAQLGGVLVLAGGNRSGAASGEAGEGTGVSDAPPTRAPTPDFCRGYFDVMGQWDPPFNCSSGDFIFCCGTCGFRFCCTFKKRRLNQSTCTNYDTPLWLNTGKPPARKDDPLHDPTKDKTNLIVYIICGVVAVMVLVGIFTKLGLEKAHRPQREHMSRALADVMRPQGHCNTDHMERDLNIVVHVQHYENMDTRTPINNLHATQMNNTVPTSPLLQQMGHPHSYPNLGQISNPYEQQPPGKELNKYASLKAVADKVSDDFYSKRRHLAELAAKGNLPLHPVRVEDEPRAFSPEHGPAKQNGQKSRTSKMPPHPLAYNSTTNFKGWDPNEQSLRRQAYGNKGKLGTAESGSSDPLGTRTQHYPPPQPYFITNSKTEVTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IMF3</accession>
    <name>A0A9L0IMF3_EQUAS</name>
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        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
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                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="Gao"/>
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                <person name="Li"/>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
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                <person name="Zhang"/>
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                <person name="C."/>
                <person name="Jin"/>
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                <person name="Yue"/>
                <person name="G."/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the polycystin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IMF3"/>
    <dbReference type="Ensembl" id="ENSEAST00005071952.1">
        <property type="protein sequence ID" value="ENSEASP00005041327.1"/>
        <property type="gene ID" value="ENSEASG00005038052.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 28"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:detection of mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="PKD1_2_channel"/>
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        <property type="entry name" value="Polycystin"/>
    </dbReference>
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        <property type="entry name" value="Polycystin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10877">
        <property type="entry name" value="POLYCYSTIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10877:SF136">
        <property type="entry name" value="POLYCYSTIN-1-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PKD_channel"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Polycystin_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
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            <end position="346"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="358"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="389"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="Polycystin" evidence="9">
        <location>
            <begin position="128"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Polycystin cation channel PKD1/PKD2" evidence="8">
        <location>
            <begin position="329"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="187"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF20519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005041327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="433" mass="49407" checksum="AC5648B9C39914B5" modified="2023-09-13" version="1">MLSLCRELYRKREVVFLTLLSSLMLNRMPWLHKEKEQQTKRILALWAKCSSSLPGSRDKNNPIYVAPAMNSPVKRLERPLKDKQLFKLTGDIVVQILFLILLVTVAYSAQNSSRFYLHQAIQKSFSHGFSEIKLLEHFYPWANRTLLPNLYGDYSGFITDGNSFLLGNVLLRQIRIPGAILFPPGVSPQEQVRPSHQDQEDKENYGVNWGPPETNITKSDSMWHYQNQETLGGYPIQGEFATYSGGGYVVRLGRNSSTANRVLQHLEQSRWLDRCTRSLFVEFVVFNANVNLFCVVTLILESNNMGAFFTSVRLDTLRSLQTSKKDFAWSVVSQVIYYLLVCYYAFMQFNLLFGWSISDYRTFFSSAVTTVGLLMGISHHKEVIALDPVLGFFLILTSVILMVLVIINLFVSAILMAFGKERRSLKVGNSMAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IMQ4</accession>
    <name>A0A9L0IMQ4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein-serine O-palmitoleoyltransferase porcupine</fullName>
            <ecNumber evidence="10">2.3.1.250</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PORCN</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[Wnt protein]-L-serine + (9Z)-hexadecenoyl-CoA = [Wnt protein]-O-(9Z)-hexadecenoyl-L-serine + CoA</text>
            <dbReference type="Rhea" id="RHEA:45336"/>
            <dbReference type="Rhea" id="RHEA-COMP:11170"/>
            <dbReference type="Rhea" id="RHEA-COMP:11171"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:61540"/>
            <dbReference type="ChEBI" id="CHEBI:85189"/>
            <dbReference type="EC" id="2.3.1.250"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the membrane-bound acyltransferase family. Porcupine subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.250" evidence="10"/>
    <dbReference type="RefSeq" id="XP_070358332.1">
        <property type="nucleotide sequence ID" value="XM_070502231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IMQ4"/>
    <dbReference type="Ensembl" id="ENSEAST00005050443.1">
        <property type="protein sequence ID" value="ENSEASP00005039050.1"/>
        <property type="gene ID" value="ENSEASG00005029924.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106827434"/>
    <dbReference type="CTD" id="64840"/>
    <dbReference type="GeneTree" id="ENSGT01030000234564"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032281">
        <property type="term" value="C:AMPA glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990698">
        <property type="term" value="F:palmitoleoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009100">
        <property type="term" value="P:glycoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030258">
        <property type="term" value="P:lipid modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099072">
        <property type="term" value="P:regulation of postsynaptic membrane neurotransmitter receptor levels"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061355">
        <property type="term" value="P:Wnt protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049941">
        <property type="entry name" value="LPLAT_7/PORCN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906">
        <property type="entry name" value="PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13906:SF12">
        <property type="entry name" value="PROTEIN-SERINE O-PALMITOLEOYLTRANSFERASE PORCUPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="197"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00040371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="461" mass="52314" checksum="8A15ABFB275A8144" modified="2023-09-13" version="1">MATFSRQEFFQQLLQGCLLPTAQQGLDQIWLLLAICLACRLLWRLGLPSYLKHASTVAGGFFSLYHFFQLHMVWVVLLSLLCYLVLFLCRHSSHRGVFLSVTILIYLLMGEMHMVDTVTWHKMRGAQMIVAMKAVSLGFDLDRGEVGAVPSPVEFMGYLYFVGTIVFGPWISFHSYLQAVQGRPLSRQWLQKVARSLALALLCLVLSTCVGPYLFPYFIPLDGDRLLRNKKRKARGTMVRWLRAYESAVSFHFSNYFVGFLSEATATLAGAGFTEEKDHLEWDLTVSKPLNVELPRSMVEVVTSWNLPMSYWLNNYVFKNALRLGTFSAVLVTYAASALLHGFSFHLAAVLLSLAFITYVEHVLRKRLARILSACVLSKRCPPDCSHQHRLGLGVRALNLLFGALAIFHLAYLGSLFDVDVDDTTEEQGYGMAYTVHKWSELSWASHWVTFGCWIFYRLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0ITK0</accession>
    <name>A0A9L0ITK0_EQUAS</name>
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        <recommendedName>
            <fullName evidence="12">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="12">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">USP2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the peptidase C19 family. USP2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="12"/>
    <dbReference type="RefSeq" id="XP_014711185.1">
        <property type="nucleotide sequence ID" value="XM_014855699.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070346185.1">
        <property type="nucleotide sequence ID" value="XM_070490084.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070346186.1">
        <property type="nucleotide sequence ID" value="XM_070490085.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070346187.1">
        <property type="nucleotide sequence ID" value="XM_070490086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0ITK0"/>
    <dbReference type="Ensembl" id="ENSEAST00005051649.1">
        <property type="protein sequence ID" value="ENSEASP00005040419.1"/>
        <property type="gene ID" value="ENSEASG00005003919.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106840315"/>
    <dbReference type="CTD" id="9099"/>
    <dbReference type="GeneTree" id="ENSGT00940000161289"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048511">
        <property type="term" value="P:rhythmic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000024">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF17">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
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    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 12" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="USP" evidence="14">
        <location>
            <begin position="268"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="80"/>
            <end position="112"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="206"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037943"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50235"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="606" mass="68105" checksum="789B8648873DB60D" modified="2023-09-13" version="1">MSQLSSTLKRYTESARYTDAPYAKSGYGTYTPSSYGANLAASFLEKEKLGFKPGPPTSLLTRPRTYGPSSILDYDRSRPLLRPDVIGGGKRPESQTWGTERPSGSGLSGGSGFPYGVTNNSLSYLPVNAHNQGVTLTQKKSNSQSDLARDFSSLRTSDSYRIDPGNLGRSPMLARTRKELCALQGLYQAASRSEYLSDYLENYGRKGSAPQVPSQTPPSRVPEVLSPTYRPSGRYTLWEKGKGQGPGSSRSSSPGRDTMNSKSAQGLAGLRNLGNTCFMNSILQCLSNTRELRDYCLQRLYMRDLSHSSNAHTALMEEFAKLIQTIWTSSPNDVVSPSEFKTQIQRYAPRFVGYNQQDAQEFLRFLLDGLHNEVNRVTVRPKSNPENLDHLPDDEKGRQMWRKYLEREDSRIGDLFVGQLKSSLTCTDCGYCSTVFDPFWDLSLPIAKRGYPEVTLMDCMRLFTKEDVLDGDEKPTCCRCRARKRCIKKFSIQRFPKILVLHLKRFSESRIRTSKLTTFVNFPLRDLDLREFASENTNHAVYNLYAVSNHSGTTMGGHYTAYCRSPVTGEWHTFNDSSVTPMSSSQVRTSDAYLLFYELASPPSRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IVS3</accession>
    <name>A0A9L0IVS3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ankyrin repeat and SOCS box protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ASB4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ankyrin SOCS box (ASB) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014708814.1">
        <property type="nucleotide sequence ID" value="XM_014853328.3"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSEAST00005065710.1">
        <property type="protein sequence ID" value="ENSEASP00005041876.1"/>
        <property type="gene ID" value="ENSEASG00005020898.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106838925"/>
    <dbReference type="KEGG" id="eai:106838925"/>
    <dbReference type="CTD" id="51666"/>
    <dbReference type="GeneTree" id="ENSGT00940000158874"/>
    <dbReference type="OMA" id="PIQYVLK"/>
    <dbReference type="OrthoDB" id="539213at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031462">
        <property type="term" value="C:Cul2-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031466">
        <property type="term" value="C:Cul5-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001214">
        <property type="term" value="P:positive regulation of vasculogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03723">
        <property type="entry name" value="SOCS_ASB4_ASB18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.750.20:FF:000001">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000193">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000271">
        <property type="entry name" value="Ankyrin repeat and SOCS box containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.750.20">
        <property type="entry name" value="SOCS box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036036">
        <property type="entry name" value="SOCS_box-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171:SF10">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 29-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24171">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 39-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00969">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158235">
        <property type="entry name" value="SOCS box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="74"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="106"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="174"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="10">
        <location>
            <begin position="251"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="SOCS box" evidence="11">
        <location>
            <begin position="379"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005041876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="426" mass="48290" checksum="14F5C9C9E69B8101" modified="2023-09-13" version="1">MDGAAAPRTKSAAAKLVKRNFLEALKLNDFGKLKAILIQRQIDVDTVFEVEDENMVLASYKQGYWLPSYKLKSSWATGLHLSVLFGHVECLLVLLDHNATINCRPNGKTPLHVACEMANLDCVKILCDRGAKLNCYSLSGHTALHFCTTPSSVLCAKQLVWRGANVNMKTNNQDEETPLHTAAHFGLAELVAFYVEHGAIVDSVNAHMETPLAIATYWALRFKEQEYSRDHHLVCRMLLDYNAEVNARDDDFKSPLHKAAWNCDHVLMHMMLEAGAEANLMDINGCAAIQYVLKVTSVRPAAQPEICYQLLLNHGAARIYPPQFHKVIQACHSYPRAIEVVVNAYEHIRWNVKWKRAIPDDDLERHWDFYHSLFTVCSNSPRTLMHLSRCAIRKTLHSRCHRVIPLLSLPLSLKKYLLLEPEGIIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IW02</accession>
    <name>A0A9L0IW02_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Speedy/RINGO cell cycle regulator family member A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">SPDYA</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J2G6"/>
    <dbReference type="Ensembl" id="ENSEAST00005072893.1">
        <property type="protein sequence ID" value="ENSEASP00005046558.1"/>
        <property type="gene ID" value="ENSEASG00005030511.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005046558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="90" mass="9793" checksum="B1D264361CCF1713" modified="2023-09-13" version="1">MASMSPPSAWPYVQDGPETRQDLCQPPEPLEPCIHSHAHMEECLSAGLQGPHPHLLVHSCVSSLLLDFGCPPNCHISSYLVLSPSPSPIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J3R5</accession>
    <name>A0A9L0J3R5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J3R5"/>
    <dbReference type="Ensembl" id="ENSEAST00005074006.1">
        <property type="protein sequence ID" value="ENSEASP00005044657.1"/>
        <property type="gene ID" value="ENSEASG00005028543.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286907"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033691">
        <property type="term" value="F:sialic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051036">
        <property type="entry name" value="SIGLEC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12035:SF139">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12035">
        <property type="entry name" value="SIALIC ACID BINDING IMMUNOGLOBULIN-LIKE LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-0430">Lectin</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040451816" description="Ig-like domain-containing protein" evidence="9">
        <location>
            <begin position="20"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="33"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="154"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="251"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005044657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="468" mass="50867" checksum="1D5393C2AE9A27E0" modified="2023-09-13" version="1">MLLLLLLLLLLLWPPLLWAAEWGKQSWDGWPGPRARDLRYQLQVQESVTVQAGLCVSVACTFSYPSDEWSDAAPAYGYWFREGTNTDRGAPAKTQGRFHLLQDIRNYSCSLDIRDAQRGDEGKYFFRVERGSYVRYNYKENQLFVHVQALTETPDIHIQETLEAGNPKNITCTAPWACERGTPPTFSWVGVALTSQGPKIPRTSVLTLTPQPQDHGSNLTCRVTLPGADVSTERTIQLNVSYAPQSLTINPGLSVLSLLPGPETLGNGSSLPVQEGQSLRLVCVANSNPPAMLSWTRGSLRLSSSSPSNPGVLELPRVELGHHGKYVCRAQHLLGSLQASLSLFVTNGENTHTSPPGSWPPHPTAGGPLFISVPGAPQNMTVHAFRGNSTGRRGLGGSEVSEVYSGDRVEVGQNALTLHPFSSSASVLPPPIPWQGHWEFLLDPSLSPLVASLSRPSLSPLGPMTSFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J788</accession>
    <name>A0A9L0J788_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial</fullName>
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        <alternativeName>
            <fullName evidence="22">Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Short-chain 3-hydroxyacyl-CoA dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">HADH</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Mitochondrial fatty acid beta-oxidation enzyme that catalyzes the third step of the beta-oxidation cycle for medium and short-chain 3-hydroxy fatty acyl-CoAs (C4 to C10). Plays a role in the control of insulin secretion by inhibiting the activation of glutamate dehydrogenase 1 (GLUD1), an enzyme that has an important role in regulating amino acid-induced insulin secretion. Plays a role in the maintenance of normal spermatogenesis through the reduction of fatty acid accumulation in the testes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(3S)-3-hydroxybutanoyl-CoA + NAD(+) = acetoacetyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30799"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57316"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(3S)-hydroxydecanoyl-CoA + NAD(+) = 3-oxodecanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31187"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62548"/>
            <dbReference type="ChEBI" id="CHEBI:62616"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(3S)-hydroxyhexadecanoyl-CoA + NAD(+) = 3-oxohexadecanoyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31159"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57349"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:62613"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57318"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="1.1.1.35"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Homodimer. Interacts with GLUD1; this interaction inhibits the activation of glutamate dehydrogenase 1 (GLUD1).</text>
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            <location evidence="1">Mitochondrion matrix</location>
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        <text evidence="3">Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.</text>
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        <property type="entry name" value="3-OHacyl-CoA_DH"/>
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        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT H"/>
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        <property type="entry name" value="ATP_synt_H"/>
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            <begin position="144"/>
            <end position="166"/>
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            <begin position="1"/>
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            <begin position="106"/>
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            <begin position="22"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
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            <begin position="106"/>
            <end position="116"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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            <dbReference type="ARBA" id="ARBA00008328"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050759.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="189" mass="20974" checksum="1C29A6DF0D4D43F5" modified="2023-09-13" version="1">RQKLTKLTRFGSSQSSPHNPKGRAGAPARVGPGRARSWAARSRCACSVPTARPAPRAAEHLRLGPAARPGHRAPGHDGALLRPPGHHLHHVLGPHRHRRALVRAERTQPRVRDARGPGHPAAPPLRCRERWPEARPAPPPPPRVIITMLVATAVCCYLFWLIAILAQLNPLFGPQLKNETIWYVRFLWE</sequence>
</entry>
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    <name>A0A9L0JD72_EQUAS</name>
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        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="14" type="primary">LOC106835784</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
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            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="14" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="gene ID" value="ENSEASG00005033633.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="7tmA_OR10A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="153"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="206"/>
            <end position="230"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="251"/>
            <end position="269"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="281"/>
            <end position="301"/>
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    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="50"/>
            <end position="299"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JEN9</accession>
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        <recommendedName>
            <fullName evidence="10">FAS-associated factor 2</fullName>
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            <fullName evidence="11">UBX domain-containing protein 8</fullName>
        </alternativeName>
    </protein>
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        <name evidence="14" type="primary">FAF2</name>
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            <taxon>Mammalia</taxon>
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    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Plays an important role in endoplasmic reticulum-associated degradation (ERAD) that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway. Involved in inhibition of lipid droplet degradation by binding to phospholipase PNPL2 and inhibiting its activity by promoting dissociation of PNPL2 from its endogenous activator, ABHD5 which inhibits the rate of triacylglycerol hydrolysis. Involved in stress granule disassembly: associates with ubiquitinated G3BP1 in response to heat shock, thereby promoting interaction between ubiquitinated G3BP1 and VCP, followed by G3BP1 extraction from stress granules and stress granule disassembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Identified in a complex that contains SEL1L, OS9, FAF2/UBXD8, UBE2J1/UBC6E and AUP1. Interacts with YOD1. Interacts (via N-terminus) with UBQLN2 (via C-terminus). Interacts with PNPLA2 and UBAC2. Interacts with ZFAND2B; probably through VCP. Interacts with LMBR1L.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lipid droplet</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014682301.1">
        <property type="nucleotide sequence ID" value="XM_014826815.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JEN9"/>
    <dbReference type="SMR" id="A0A9L0JEN9"/>
    <dbReference type="Ensembl" id="ENSEAST00005044690.1">
        <property type="protein sequence ID" value="ENSEASP00005051649.1"/>
        <property type="gene ID" value="ENSEASG00005012004.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106821922"/>
    <dbReference type="CTD" id="23197"/>
    <dbReference type="GeneTree" id="ENSGT00940000157197"/>
    <dbReference type="OMA" id="ILIRHQW"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035473">
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0055102">
        <property type="term" value="F:lipase inhibitor activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035617">
        <property type="term" value="P:stress granule disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd02991">
        <property type="entry name" value="UAS_ETEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14414">
        <property type="entry name" value="UBA_FAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16120">
        <property type="entry name" value="UBX_UBXN3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000101">
        <property type="entry name" value="FAS-associated factor 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000066">
        <property type="entry name" value="FAS-associated factor 2 isoform X2"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.10.8.10:FF:000043">
        <property type="entry name" value="Fas-associated factor family member 2"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049483">
        <property type="entry name" value="FAF1_2-like_UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006577">
        <property type="entry name" value="UAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054109">
        <property type="entry name" value="UBA_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050730">
        <property type="entry name" value="UBX_domain-protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322:SF1">
        <property type="entry name" value="FAS-ASSOCIATED FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23322">
        <property type="entry name" value="FAS-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21021">
        <property type="entry name" value="FAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22566">
        <property type="entry name" value="UBA_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00789">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00594">
        <property type="entry name" value="UAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50033">
        <property type="entry name" value="UBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UBX" evidence="13">
        <location>
            <begin position="357"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="299"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="303"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    <evidence type="ECO:0000256" key="3">
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            <dbReference type="ARBA" id="ARBA00004502"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00055454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005051649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="445" mass="52623" checksum="A6C7FC018E566ED9" modified="2023-09-13" version="1">MAAPEDRDLTQEQTEKLLQFQDLTGIESMDQCRHTLEQHNWNIEAAVQDRLNEQEGVPSVFNPPPSRPLQVNTADHRIYSYVVSRPQPRGLLGWGYYLIMLPFRFTYYTILDIFRFALRFIRPDPRSRVTDPVGDIVSFMHSFEEKYGRAHPVFYQGTYSQALNDAKRELRFLLVYLHGDDHQDSDEFCRNTLCAPEVISLINTRMLFWACSTNKPEGYRVSQALRENTYPFLAMIMLKDRRMTVVGRLEGLIQPDDLINQLTFIMDANQTYLVSERLEREERNQTQVLRQQQDEAYLASLRADQEKERKKREERERKRRKEEEVQQQKLAEERRRRNLQEEKERKLECLPPEPSPDDPESVKIIFKLPNDSRVERRFHFSQSLTVIHDFLFSLKESPEKFQIEANFPRRVLPCVPSEEWPNPPTLQEAGLSHTEVLFVQDLTDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JKU7</accession>
    <name>A0A9L0JKU7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein 4.1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">4.1R</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Band 4.1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Erythrocyte membrane protein band 4.1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">EPB41</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Protein 4.1 is a major structural element of the erythrocyte membrane skeleton. It plays a key role in regulating membrane physical properties of mechanical stability and deformability by stabilizing spectrin-actin interaction. Recruits DLG1 to membranes. Required for dynein-dynactin complex and NUMA1 recruitment at the mitotic cell cortex during anaphase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_070366656.1">
        <property type="nucleotide sequence ID" value="XM_070510555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JKU7"/>
    <dbReference type="Ensembl" id="ENSEAST00005075061.1">
        <property type="protein sequence ID" value="ENSEASP00005049815.1"/>
        <property type="gene ID" value="ENSEASG00005010160.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106839131"/>
    <dbReference type="CTD" id="2035"/>
    <dbReference type="GeneTree" id="ENSGT00940000157833"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13184">
        <property type="entry name" value="FERM_C_4_1_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17105">
        <property type="entry name" value="FERM_F1_EPB41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000001">
        <property type="entry name" value="Erythrocyte membrane protein band 4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000001">
        <property type="entry name" value="Erythrocyte membrane protein band 4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000002">
        <property type="entry name" value="Erythrocyte protein band 4.1-like 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008379">
        <property type="entry name" value="Band_4.1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021187">
        <property type="entry name" value="EPB4.1_FERM_F1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007477">
        <property type="entry name" value="SAB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF12">
        <property type="entry name" value="PROTEIN 4.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05902">
        <property type="entry name" value="4_1_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08736">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04382">
        <property type="entry name" value="SAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002304">
        <property type="entry name" value="Membrane_skeletal_4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01195">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="9" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FERM" evidence="20">
        <location>
            <begin position="211"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="518"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="710"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="49"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="584"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="710"/>
            <end position="720"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00054563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00078357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005049815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="861" mass="96565" checksum="9FEFDC254EC5F2D5" modified="2023-09-13" version="1">MTTEKSLVAEAENSQHQEQNEEGEGATNSGQQETRLEETSQAAAEGDNQCEQKLKTSNGDTPTHEDLTKNKERTSENRGLSRLFSSLLKRPKSQVSEEEGKEVESGTEKGEGGQREREFGTSIDEEIILKAPIAAPEPELKTDPSLDLHSLSSAETQPAQEERREDPDFETKEEEGLEECSKIEVKEESPESKAERELKASQKSIRRHRNMHCKVSLLDDTVYECVVEKHAKGQDLHKRVCEHLNLLEEDYFGLAIWDNTTSKTWLDPAKEIKKQVRGVPWNFTFNVKFYPPDPAQLTEDITRYYLCLQLRQDIVAGRLPCSFATLALLGSYTVQSELGDYDPELHGADYVSDFKLAPNQTKELEEKVMELHKSYRSMTPAQADLEFLENAKKLSMYGVDLHKAKDLEGVDIILGVCSSGLLVYKDKLRINRFPWPKVLKISYKRSSFFIKIRPGEQEQYESTIGFKLPSYRAAKKLWKVCVEHHTFFRLTSTDTIPKSKFLALGSKFRYSGRTQAQTRQASALIDRPAPHFERTASKRASRSLDGAAAVHSADRSPRPTSAPAIAQSQVTEGAPVKKAGVSKAQKETAEAEVKGEEVPPEQAEPEPTEVQKVEKTHIEVTVPTSNGDQTQKLAEKTEDLIRMRKKKRERLDGENIYIRHSNLMLEDLGKSQEEIKKHHASISELKKNFMESVPEPRPSEWDKRLSTHSPFRTPNINGQIPTGEGPPLVKTQTVTISDTANAVKSEIPTKDVPIVHTETKTITYEAAQTDDSNGDLDPGVLLSAQTITSETTSSTTTTQITKTVKGGISETRIEKRIVITGDADIDHDQVLVQAIKEAKEQHPDMSVTKVVVHQETEISEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JKX9</accession>
    <name>A0A9L0JKX9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Metallothionein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MT1X</name>
        <name evidence="7" type="synonym">LOC106822242</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Metallothioneins have a high content of cysteine residues that bind various heavy metals.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the metallothionein superfamily. Type 1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallothionein superfamily. Type 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JKX9"/>
    <dbReference type="Ensembl" id="ENSEAST00005056984.1">
        <property type="protein sequence ID" value="ENSEASP00005050617.1"/>
        <property type="gene ID" value="ENSEASG00005030801.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182967"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071276">
        <property type="term" value="P:cellular response to cadmium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071280">
        <property type="term" value="P:cellular response to copper ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036018">
        <property type="term" value="P:cellular response to erythropoietin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071294">
        <property type="term" value="P:cellular response to zinc ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010273">
        <property type="term" value="P:detoxification of copper ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.10.10:FF:000001">
        <property type="entry name" value="Metallothionein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.10.10">
        <property type="entry name" value="Metallothionein Isoform II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017854">
        <property type="entry name" value="Metalthion_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023587">
        <property type="entry name" value="Metalthion_dom_sf_vert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001008">
        <property type="entry name" value="Metalthion_mollusc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000006">
        <property type="entry name" value="Metalthion_vert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018064">
        <property type="entry name" value="Metalthion_vert_metal_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23299">
        <property type="entry name" value="METALLOTHIONEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23299:SF59">
        <property type="entry name" value="METALLOTHIONEIN-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00131">
        <property type="entry name" value="Metallothio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00875">
        <property type="entry name" value="MTMOLLUSC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57868">
        <property type="entry name" value="Metallothionein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00203">
        <property type="entry name" value="METALLOTHIONEIN_VRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0104">Cadmium</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0480">Metal-thiolate cluster</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="71" mass="6978" checksum="C6A12E6B6BB44887" modified="2023-09-13" version="1">MDPNCSCVAGESCTCAGSCKCKQCRCASCKKTLISAALSSPGCCSCCPVGCAKCAQGCVCKGASDKCSCCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JLH9</accession>
    <name>A0A9L0JLH9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JLH9"/>
    <dbReference type="Ensembl" id="ENSEAST00005040745.1">
        <property type="protein sequence ID" value="ENSEASP00005053238.1"/>
        <property type="gene ID" value="ENSEASG00005024355.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 6"/>
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        <property type="term" value="C:immunoglobulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd04980">
        <property type="entry name" value="IgV_L_kappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001378">
        <property type="entry name" value="Immunoglobulin kappa variable 4-1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ig-like_dom"/>
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    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
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    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050150">
        <property type="entry name" value="IgV_Light_Chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23267">
        <property type="entry name" value="IMMUNOGLOBULIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-1280">Immunoglobulin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="5">
        <location>
            <begin position="5"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00043265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005053238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="198" mass="21434" checksum="6257E9C23DE90F81" modified="2023-09-13" version="1">MVPRPQFLILLVFWVSGVCGHIVMTQSPGSLAVSVEDSVTINCKSSQSLFGSSNQKNYLAWYQQKPGQAPKLLIYWASSRASGVSDQFSGSGSGTDFTLTISSLQAEDVAVYCCQQEYSSPPTVLQPQRQTSSPKPWVSESLLHQLLLSCTCLRGPSAIRKAMSPVLIVEECVSLMECLSRLACSASFAEGSHSMKTL</sequence>
</entry>
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    <accession>A0A9L0JNS5</accession>
    <name>A0A9L0JNS5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 177</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123285332</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="J."/>
                <person name="Fang"/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the early steps of cytochrome c oxidase subunit II (MT-CO2/COX2) maturation and is required for the stabilization of COX20 and the newly synthesized MT-CO2/COX2 protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM177 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JNS5"/>
    <dbReference type="Ensembl" id="ENSEAST00005074818.1">
        <property type="protein sequence ID" value="ENSEASP00005054048.1"/>
        <property type="gene ID" value="ENSEASG00005024571.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010354"/>
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        <property type="component" value="Chromosome 4"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026620">
        <property type="entry name" value="TMEM177"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR21824:SF4">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00003998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005054048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="312" mass="33728" checksum="A7A4CD07B0B90CC4" modified="2023-09-13" version="1">MAGPLWRASAFVQRHRTGLLVGSCAGLFGAQISYHLFPDPVVQWLYQYWPQGQPAPLSPELQRLFQEVLQDMGVPSGHCYEAFTTFTFQPVSAGFPRLPAGAVVGIPASFLGGPVTHSDQPVIIHGHRVNWQSPSGARLRDALTLSRDAQKFALAREVVYLESAAAAALQALPAPACLAGTWALGVGAKHALGLYGGPMNLRAAFNLVAAVAGFVAYAFSTDSLTHALEAWLDRRTASLSAAYARGGVEFYEKVLSGNLALRGLLGRPGEKLYTPSGNVAPRHWFRIKHLPYTTRRDSVLQLWRATLTPGRS</sequence>
</entry>
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    <accession>A0A9L0JPZ3</accession>
    <name>A0A9L0JPZ3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106841236</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Cell membrane</location>
            <topology evidence="2 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JPZ3"/>
    <dbReference type="Ensembl" id="ENSEAST00005069005.1">
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    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 14" id="KW-0297">G-protein coupled receptor</keyword>
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    <sequence length="310" mass="35137" checksum="E7521AF8A13F7868" modified="2023-09-13" version="1">MKNKTMLTEFILLGLTDAPEMQVVVFTFLLLAYLLSIIGNLTILILTLLDPHLQTPMYFFLRNFSFLEISFTNIFIPRVLISITTGNKSISFAGCFTQYFFAIFLGATEFYLLAAMSYDRYVAVCKPLHYTTMMSNRVCTQLVLCSWLAGLMVIIVPITLMSQQDFCASNRLNHYFCDYEPLRELSCSDTSLIEKIVFSVASVTLVATLVLVVLSYTFIIRTILKLPSAQQRTKAFSTCSSHMIVITLSYGSCFFVYLKPSTKEPGTFNKGVALLFTSVAPLLNPFIYTLRNQQVKQAFKDTVQKLMNLY</sequence>
</entry>
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    <accession>A0A9L0JQ07</accession>
    <name>A0A9L0JQ07_EQUAS</name>
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        <recommendedName>
            <fullName evidence="3 9">MICOS complex subunit MIC13</fullName>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
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                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Fang"/>
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                <person name="Zhao"/>
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                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Guo"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
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                <person name="Zhuang"/>
                <person name="G."/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the mitochondrial contact site and cristae organizing system (MICOS) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the MICOS complex subunit Mic13 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014843135.3"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="Mic13"/>
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        <property type="entry name" value="MICOS COMPLEX SUBUNIT MIC13"/>
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    </dbReference>
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        <property type="entry name" value="QIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00006771"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00018172"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="118" mass="13224" checksum="AE3D23844A161B34" modified="2023-09-13" version="1">MVPRVWSLMRFLLKGSVAGVAVYLVYDQELLGPSEKTQAVLQKAEEVVPPAVYGFSQYVCDQTGLKVPQLPAPPKFNFHLRDSWNSGIMTVMSALSVAPSKACEYSKEGWQYLKERIK</sequence>
</entry>
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    <accession>A0A9L0JQA9</accession>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name evidence="2" type="primary">LOC106848519</name>
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            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="J."/>
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                <person name="Zhao"/>
                <person name="Z."/>
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                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
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                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JQA9"/>
    <dbReference type="Ensembl" id="ENSEAST00005051543.1">
        <property type="protein sequence ID" value="ENSEASP00005055506.1"/>
        <property type="gene ID" value="ENSEASG00005001317.2"/>
    </dbReference>
    <dbReference type="KEGG" id="eai:106848519"/>
    <dbReference type="GeneTree" id="ENSGT00420000030724"/>
    <dbReference type="OrthoDB" id="8989119at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005055506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="268" mass="29530" checksum="5B7BAC921AC26680" modified="2023-09-13" version="1">MCQALVLSILHKFTHFTVTIVLRPRNYFHSHFTDLETKAQVFWASACVHTLIIMRLGVGLNSGACVGPVDINHVPWGWAFVHQAFTKPHLARMCGWPGTHSSVKGLAHGSLCSCAVRAGRGGSGEASPRLSFTECPEGSVTSLNPRWDLHRGSVSVPFHRGRPRGPERLGNLPEGTHAGEGLSQVGFQLQASISEAKATAMPHFLDWFVPVYLVISVLILVGFGACIYYFEPGLQEAHKWRMQRPLVDRDLRKTLMVRDNLAFGGPEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JVK0</accession>
    <name>A0A9L0JVK0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106836013</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014704108.1">
        <property type="nucleotide sequence ID" value="XM_014848622.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JVK0"/>
    <dbReference type="Ensembl" id="ENSEAST00005074734.1">
        <property type="protein sequence ID" value="ENSEASP00005054047.1"/>
        <property type="gene ID" value="ENSEASG00005029375.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106836013"/>
    <dbReference type="KEGG" id="eai:106836013"/>
    <dbReference type="GeneTree" id="ENSGT01040000240383"/>
    <dbReference type="OMA" id="FNVINHY"/>
    <dbReference type="OrthoDB" id="9440924at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15406">
        <property type="entry name" value="7tmA_OR8D-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000004">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005054047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="310" mass="34707" checksum="CDBE3D5D6A84B4BB" modified="2023-09-13" version="1">MALGNHSTVTEFILAGLTEEPELQVPLLLLFLGIYVVTVMGNLGMITLIGLSSHLHTPMYYFLSSLSFTDLCHSTVITPKMLVNFVTEKNVISYPQCMTQLYFFLVFAISECYMLAAMAYDRYVAICSPLLYNVIMSHQVCFSLILGVYIIGLICAFAHTGCMLRVNFCKFYVINHYFCDLLPLLKLSCSSTDVNELLILCVGTFNIFAPSLTILSSYIFIISRILHIHSTEGRSKAFSTCSSHMLAVLLFFGSCAFMYLQPSSVGSMDQGKVSSVFYTIVVPMLNPLIYSLRNKDVNVALKKMLKSRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JWP3</accession>
    <name>A0A9L0JWP3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Zinc finger and BTB domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">ZBTB1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Acts as a transcriptional repressor. Represses cAMP-responsive element (CRE)-mediated transcriptional activation. In addition, has a role in translesion DNA synthesis. Requires for UV-inducible RAD18 loading, PCNA monoubiquitination, POLH recruitment to replication factories and efficient translesion DNA synthesis. Plays a key role in the transcriptional regulation of T lymphocyte development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044629671.1">
        <property type="nucleotide sequence ID" value="XM_044773736.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070369870.1">
        <property type="nucleotide sequence ID" value="XM_070513769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JWP3"/>
    <dbReference type="Ensembl" id="ENSEAST00005065331.1">
        <property type="protein sequence ID" value="ENSEASP00005053540.1"/>
        <property type="gene ID" value="ENSEASG00005031939.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106840112"/>
    <dbReference type="CTD" id="22890"/>
    <dbReference type="GeneTree" id="ENSGT00940000157501"/>
    <dbReference type="OMA" id="EDDWPEA"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18192">
        <property type="entry name" value="BTB_POZ_ZBTB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000109">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF42">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="20">
        <location>
            <begin position="24"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="216"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="578"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="21">
        <location>
            <begin position="606"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="160"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="265"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    <evidence type="ECO:0000256" key="8">
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            <dbReference type="ARBA" id="ARBA00022782"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059022"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
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            <dbReference type="ARBA" id="ARBA00074852"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
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    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005053540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="644" mass="73753" checksum="53EE40B6D23354B3" modified="2023-09-13" version="1">MAKPSHSSYVLQQLNNQREWGFLCDCCIAIDDIYFQAHKAVLAACSSYFRMFFMNHQHSTAQLNLSNMKISAECFDLILQFMYLGKIMTAPSSFEQFKVAMNYLQLYNVPDCLEDIQDADCSSSKCSSSASSKQNSKMIFGVRMYEDTVARNGNEANRWCAEPSSTVNTPHNREPDEESLQLGNFPEPLFDVCKKSSVSKLSTPKERVSRRFGRSFTCDSCGFGFSCEKLLDEHVLTCTNRHSYQNTRSYHRIVDIRDGKDSNIKAEFGEKDSSKTFSAQTDKYRGDTSQAADDSTSTTGSRKSSTVESELASEEKSRAAERKRIIIKMEPEDIPTDELKDFNIIKVTDKDCNESTDNDELEDEPEEPFYRYYVEEDVSIKKSGRKTLKPRMSINADERGGLENLRPPNNSSPVQEDTENASCELCGLTITEEDLSSHYLAKHIENICACGKCGQILVKGRQLQEHAQRCGEPQDLTMNGLGNTEEKMDMEENPDEQSEIRDMFVEMLDDFRDSHFQINSIQKKQLFKHSACPFRCPNCGQRFETENLVVEHMSSCLDQDMFKSAILEENERDHRRKHFCNLCGKGFYQRCHLREHYTVHTKEKQFVCQTCGKQFLRERQLRLHNDMHKGMASGEIGPSKLLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JZ64</accession>
    <name>A0A9L0JZ64_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">UPAR/Ly6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LY6D</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JZ64"/>
    <dbReference type="Ensembl" id="ENSEAST00005049551.1">
        <property type="protein sequence ID" value="ENSEASP00005057803.1"/>
        <property type="gene ID" value="ENSEASG00005030371.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111514"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030098">
        <property type="term" value="P:lymphocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23542">
        <property type="entry name" value="TFP_LU_ECD_Ly6D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000003">
        <property type="entry name" value="lymphocyte antigen 6E isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018363">
        <property type="entry name" value="CD59_antigen_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016054">
        <property type="entry name" value="LY6_UPA_recep-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042339">
        <property type="entry name" value="Ly6D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035076">
        <property type="entry name" value="Toxin/TOLIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16982">
        <property type="entry name" value="LYMPHOCYTE ANTIGEN 6D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16982:SF2">
        <property type="entry name" value="LYMPHOCYTE ANTIGEN 6D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00087">
        <property type="entry name" value="Toxin_TOLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00134">
        <property type="entry name" value="LU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00983">
        <property type="entry name" value="LY6_UPAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="10">
        <location>
            <begin position="139"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005057803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="246" mass="26119" checksum="84FECF69571656EC" modified="2023-09-13" version="1">MQGRGWKIRDSMIQERTEGWREEDVAVSCRVSGGLCPQPGEGEADPCPGRRRPEAEVPPAGRARQVLGRGCGPARVGQVPVERCCLADCPPPPLLPTPVPAIRPPKRRGPHCSQRRPEMKTALLLLVALAVAAGPAQALRCHVCSNSMNCKKPQTCSAGSRYCRTMSKMEPLSGNLVEKDCVESCTPTHGLQSQVSRGTAVTLCCQEDLCNENLQSSAPARTLPTGATLSLALALGLVALILGPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K3C1</accession>
    <name>A0A9L0K3C1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mRNA(cytosine(6666)) deaminase</fullName>
            <ecNumber evidence="4">3.5.4.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">mRNA(cytosine(6666)) deaminase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">APOBEC2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Probable C to U editing enzyme whose physiological substrate is not yet known. Does not display detectable apoB mRNA editing. Has a low intrinsic cytidine deaminase activity. May play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>cytidine(6666) in apoB mRNA + H2O + H(+) = uridine(6666) in apoB mRNA + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:21772"/>
            <dbReference type="Rhea" id="RHEA-COMP:13888"/>
            <dbReference type="Rhea" id="RHEA-COMP:13889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="3.5.4.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytidine and deoxycytidylate deaminase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.36" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0K3C1"/>
    <dbReference type="Ensembl" id="ENSEAST00005038058.1">
        <property type="protein sequence ID" value="ENSEASP00005056842.1"/>
        <property type="gene ID" value="ENSEASG00005037408.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156616"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004126">
        <property type="term" value="F:cytidine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016554">
        <property type="term" value="P:cytidine to uridine editing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016556">
        <property type="term" value="P:mRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044029">
        <property type="term" value="P:positive regulation of gene expression via chromosomal CpG island demethylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000031">
        <property type="entry name" value="Probable C-&gt;U-editing enzyme APOBEC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013158">
        <property type="entry name" value="AID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050610">
        <property type="entry name" value="APOBEC_Cyt_Deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13857:SF4">
        <property type="entry name" value="C-U-EDITING ENZYME APOBEC-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13857">
        <property type="entry name" value="MRNA EDITING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08210">
        <property type="entry name" value="APOBEC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="13">
        <location>
            <begin position="64"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005056842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="205" mass="23206" checksum="2A97ED19A2F747DD" modified="2023-09-13" version="1">MAQKEESPEAAAPTSQNGEDLEDLDDPEKLKELIELPPFEIVTGERLPVNFFKFQFRNVEYSSGRNKTFLCYVVEAQSKGGQVQASRGYLEDEHAAAHAEEAFFNTIMPAFDPALRYNVTWYVSSSPCAACADRIIKTLSKTKNLRLLILVGRLFMWEEPEIQAALKKLKEAGCRLRIMKPQDFEYVCLLPPRDSSDMYGHPGHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K4Z0</accession>
    <name>A0A9L0K4Z0_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transducer of ERBB2, 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">TOB2</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BTG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014719871.2">
        <property type="nucleotide sequence ID" value="XM_014864385.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044634976.1">
        <property type="nucleotide sequence ID" value="XM_044779041.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070362915.1">
        <property type="nucleotide sequence ID" value="XM_070506814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K4Z0"/>
    <dbReference type="Ensembl" id="ENSEAST00005038350.1">
        <property type="protein sequence ID" value="ENSEASP00005034844.1"/>
        <property type="gene ID" value="ENSEASG00005031738.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005053460.1">
        <property type="protein sequence ID" value="ENSEASP00005058423.1"/>
        <property type="gene ID" value="ENSEASG00005031738.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005058105.1">
        <property type="protein sequence ID" value="ENSEASP00005056405.1"/>
        <property type="gene ID" value="ENSEASG00005031738.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005058419.1">
        <property type="protein sequence ID" value="ENSEASP00005042378.1"/>
        <property type="gene ID" value="ENSEASG00005031738.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005069862.1">
        <property type="protein sequence ID" value="ENSEASP00005049961.1"/>
        <property type="gene ID" value="ENSEASG00005031738.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005072862.1">
        <property type="protein sequence ID" value="ENSEASP00005035380.1"/>
        <property type="gene ID" value="ENSEASG00005031738.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106845928"/>
    <dbReference type="KEGG" id="eai:106845928"/>
    <dbReference type="CTD" id="10766"/>
    <dbReference type="GeneTree" id="ENSGT00940000154208"/>
    <dbReference type="OMA" id="NGGISYM"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042809">
        <property type="term" value="F:nuclear vitamin D receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045671">
        <property type="term" value="P:negative regulation of osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030316">
        <property type="term" value="P:osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045778">
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        <property type="project" value="Ensembl"/>
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    <dbReference type="PANTHER" id="PTHR17537">
        <property type="entry name" value="TRANSDUCER OF ERBB2 TOB"/>
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        <property type="entry name" value="BTG"/>
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        <property type="entry name" value="PAM2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00310">
        <property type="entry name" value="ANTIPRLFBTG1"/>
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        <property type="entry name" value="BTG_1"/>
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    </dbReference>
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        <property type="entry name" value="BTG_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anti-proliferative protein" evidence="4">
        <location>
            <begin position="40"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Anti-proliferative protein" evidence="5">
        <location>
            <begin position="86"/>
            <end position="105"/>
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    </feature>
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            <begin position="144"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="191"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="145"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="194"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01203"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005056405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="345" mass="36658" checksum="49CACD5B6F008900" modified="2023-09-13" version="1">MQLEIKVALNFIISYLYNKLPRRRADLFGEELERLLKKKYEGHWYPDKPLKGSGFRCVHIGEIVDPVVELAAKRSGLAVEDVRANVPEELSVWIDPFEVSYQIGEKGAVKVLYLDDSEGCVAPELDKEIKSSFNPDAQVFVPIGSQDSSLSNSPSPSFGQSPSPTFIPRSAQPITFTTASFAATKFGSTKMKKGGGAASGGGVAGSGSGGQQPSQQQPRMARSPTNSLLKHKSLSLSVHSLNFITASPAPQSQLSPNAKEFVYNGAGSPSLFFDGADGQGSGTPASFGGSGAGTCNSSSFDVAQVFGGGTNSLFLEKTPFVEGLSYNLNTMQYPSQPFQPVVLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K8R8</accession>
    <name>A0A9L0K8R8_EQUAS</name>
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        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106848129</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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    <comment type="subcellular location">
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            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
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            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="gene ID" value="ENSEASG00005038014.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 20"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15234">
        <property type="entry name" value="7tmA_OR7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48001">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
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    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
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    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="26"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="140"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="197"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="237"/>
            <end position="260"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="312" mass="34141" checksum="EFF75A44968FA9B1" modified="2023-09-13" version="1">MEAGNHTEVSEFLLLGLSEDPELQSLLFGVSLSMYLVTVLGNLLIILAVSSDSRLHTPMYFFLSNLSFVDICFISTTIPKMLVNIQAQSTDISYIGCLAQVCFSTIFAGMDNCLLSVMAYDRFVAICHPLHYMVIMNPRLCGLLVLMSWFIILSVSLIDILLMMQLTFCIGTEIPNFFCELVQVLRVACSDTLINNIFLYVATALLGVFPLTGIIFSYSQIVFSLMRMSSAGGKYKAFSTCGSHLSAVTLFYGTGLGVYLSSAVTHSSQRSSIASVMYTVVTPMLNPFIYSLRNKDVKGALGRLLGSVASSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K979</accession>
    <name>A0A9L0K979_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K979"/>
    <dbReference type="Ensembl" id="ENSEAST00005069894.1">
        <property type="protein sequence ID" value="ENSEASP00005058947.1"/>
        <property type="gene ID" value="ENSEASG00005024868.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000163997"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
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    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
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        <property type="entry name" value="Butyrophilin subfamily 1 member A1"/>
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        <property type="entry name" value="Immunoglobulins"/>
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        <property type="entry name" value="BTN3A2-like_Ig-C"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR24100:SF133">
        <property type="entry name" value="BUTYROPHILIN-LIKE PROTEIN 10 PSEUDOGENE-RELATED"/>
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    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="17"/>
            <end position="138"/>
        </location>
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    <feature type="domain" description="Ig-like" evidence="7">
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        <source>
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        </source>
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            <dbReference type="Ensembl" id="ENSEASP00005058947.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="269" mass="29343" checksum="59D6ABAABAAB52A5" modified="2023-09-13" version="1">MAKTHGQDAFRPIGSVPLLLLQLLSGRKADFEVLGPSEPVWAMVGGNVDLPCYLSPNISAEDMELRWYRDQPSPAVHVRRKGKDVPEEQMALYQGRTTFLKDGVAQGRASVRIHNVTTFDNGMFHCQFNDSTVSAEATLWLTVAGLGSEPRIQVQVDQDGVVRAQCTSAGWYPEPQVEWRDFQGQMLPSETNVSVLPTTGLWAVTSSLTVPNKAVGGLACSVSSPTFPEKKVSRHHLSGECSVLTSKALSTGARVPGCQDIRRREPQGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K9K6</accession>
    <name>A0A9L0K9K6_EQUAS</name>
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        <recommendedName>
            <fullName evidence="11">ATP-dependent RNA helicase DHX29</fullName>
            <ecNumber evidence="11">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">DEAH box protein 29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 15" type="primary">DHX29</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-binding RNA helicase involved in translation initiation. Part of the 43S pre-initiation complex that is required for efficient initiation on mRNAs of higher eukaryotes with structured 5'-UTRs by promoting efficient NTPase-dependent 48S complex formation. Specifically binds to the 40S ribosome near the mRNA entrance. Does not possess a processive helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Part of the 43S pre-initiation complex (PIC) that contains at least Met-tRNA, EIF1, EIF1A (EIF1AX or EIF1AY), EIF2S1, EIF2S2, EIF2S3, EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L, EIF3M, DHX29 and the 40S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="11"/>
    <dbReference type="Ensembl" id="ENSEAST00005081448.1">
        <property type="protein sequence ID" value="ENSEASP00005062044.1"/>
        <property type="gene ID" value="ENSEASG00005013287.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106843359"/>
    <dbReference type="KEGG" id="eai:106843359"/>
    <dbReference type="CTD" id="54505"/>
    <dbReference type="GeneTree" id="ENSGT00940000157286"/>
    <dbReference type="OMA" id="SWFANMS"/>
    <dbReference type="OrthoDB" id="5600252at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043024">
        <property type="term" value="F:ribosomal small subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008494">
        <property type="term" value="F:translation activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042255">
        <property type="term" value="P:ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd17975">
        <property type="entry name" value="DEXHc_DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000325">
        <property type="entry name" value="ATP-dependent RNA helicase DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000500">
        <property type="entry name" value="ATP-dependent RNA helicase DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000002">
        <property type="entry name" value="Putative ATP-dependent RNA helicase DHX36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03068">
        <property type="entry name" value="DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034730">
        <property type="entry name" value="DHX29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056328">
        <property type="entry name" value="DSRM_DHX29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059023">
        <property type="entry name" value="RNA_hel_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056890">
        <property type="entry name" value="UBA_DHX29-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF264">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24385">
        <property type="entry name" value="DSRM_DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26026">
        <property type="entry name" value="RNA_hel_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24899">
        <property type="entry name" value="UBA_DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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            <begin position="1"/>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KAI9</accession>
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        <name evidence="8" type="primary">CBLN4</name>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="A."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C1q_dom"/>
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        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
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        <property type="entry name" value="CEREBELLIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="COMPLEMNTC1Q"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="chain" id="PRO_5040300438" evidence="6">
        <location>
            <begin position="28"/>
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    </feature>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KBM1</accession>
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        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
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                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Pan"/>
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                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="protein sequence ID" value="ENSEASP00005062213.1"/>
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        <property type="component" value="Chromosome 19"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:olfactory receptor activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="entry name" value="Olfact_rcpt"/>
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        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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        <property type="entry name" value="OLFACTORYR"/>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
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    <keyword evidence="7 12" id="KW-0297">G-protein coupled receptor</keyword>
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    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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            <begin position="30"/>
            <end position="54"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="66"/>
            <end position="85"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="105"/>
            <end position="124"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="136"/>
            <end position="162"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="201"/>
            <end position="229"/>
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    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
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            <begin position="46"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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        <source>
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            <dbReference type="Ensembl" id="ENSEASP00005062213.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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        <submittedName>
            <fullName evidence="9">PDS5 cohesin associated factor B</fullName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        <text evidence="2">Belongs to the PDS5 family.</text>
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    <dbReference type="Ensembl" id="ENSEAST00005041186.1">
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        <property type="component" value="Chromosome 9"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0051301">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="CDD" id="cd19953">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.25.10.10:FF:001146">
        <property type="entry name" value="PDS5 cohesin associated factor B"/>
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    <dbReference type="FunFam" id="1.25.10.10:FF:000064">
        <property type="entry name" value="Sister chromatid cohesion protein PDS5 homolog A"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039776">
        <property type="entry name" value="Pds5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663">
        <property type="entry name" value="ANDROGEN INDUCED INHIBITOR OF PROLIFERATION AS3 / PDS5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12663:SF1">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN PDS5 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20168">
        <property type="entry name" value="PDS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1246"/>
            <end position="1570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1266"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1285"/>
            <end position="1296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1301"/>
            <end position="1313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1325"/>
            <end position="1343"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1354"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1374"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
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    <feature type="compositionally biased region" description="Basic residues" evidence="8">
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            <begin position="1439"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1471"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1484"/>
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        <location>
            <begin position="1508"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1552"/>
            <end position="1562"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00006254"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1570" mass="176540" checksum="405FCCFE1F447277" modified="2023-09-13" version="1">MWLSAKVKEAAEAEEALTAAALAASAATAAARPWGCVRARRGGAGRGGSPAGGGRAQARGAVWSAGGAVRPRRALPAPGCCPRRFDWSSRRRALGAILKRRGGGTAGRLDAAATADPGEQRSERCSEAGTRRGKNISIMAHSKARTNDGKITYPPGVKEISDKISKEEMVRRLKMVVKTFMDMDQDSEEEKELYLNLALHLASDFFLKHPDKDVRLLVACCLADIFRIYAPEAPYTSPDKLKDIFMFITRQLKGLEDTKSPQFNRYFYLLENIAWVKSYNICFELEDSNEIFTQLYRTLFSVINNGHNQKVHMHMVDLMSSIICEGDTVSQELLDTVLVNLVPAHKNLNKQAYDLAKALLKRTAQAIEPYITNFFNQVLMLGKTSISDLSEHVFDLILELYNIDSHLLLSVLPQLEFKLKSNDNEERLQVVKLLAKMFGAKDSELASQNKPLWQCYLGRFNDIHVPIRLECVKFASHCLMNHPDLAKDLTEYLKVRSHDPEEAIRHDVIVSIVTAAKKDILLVNDHLLNFVRERTLDKRWRVRKEAMMGLAQIYKKYALQSAAGKDAAKQISWIKDKLLHIYYQNSIDDRLLVERIFAQYMVPHNLETTERMKCLYYLYATLDLNAVKALNEMWKCQNLLRHQVKDLLDLIKQPKTDASVKAIFSKVMVITRNLPDPGKAQDFMKKFTQVLEDDEKIRKQLEVLVSPTCSCKQAEGCVREITKKLGNPKQPTNPFLEMIKFLLERIAPVHIDTESISALIKQVNKSIDGTADDEDEGVPTDQAIRAGLELLKVLSFTHPISFHSAETFESLLACLKMDDEKVAEAALQIFKNTGSKIEEDFPHIRSALLPVLHHKSKKGPPRQAKYAIHCIHAIFSSKETQFAQIFEPLHKSLDPSNLEHLITPLVTIGHIALLAPDQFAAPLKSLVATFIVKDLLMNDRLPGKKTTKLWVPDEEVSPETMVKVSNMNENNHSKSGTSTLRLLTTILHSDGDLTEQGKISKPDMSRLRLAAGSAIVKLAQEPCYHEIITLEQYQLCALAINDECYQVRQVFAQKLHKGLSRLRLPLEYMAICALCAKDPVKERRAHARQCLVKNINVRREYLKQHAAVSEKLLSLLPEYVVPYTIHLLAHDPDYVKVQDIEQLKDVKECLWFVLEILMAKNENNSHAFIRKMVENIKQTKDAQGPDDAKMNEKLYTVCDVAMNIIMSKSTTYSLESPKDPVLPARFFTQPDKNFSNTKNYLPPEMKSFFTPGKPKTTNVLGAVNKPLSSAGKQSQTKSSRMETVSNASSSSNPSSPGRIKGRLDSSEMDHSENEDYTMSSPLPGKKSDKRDDSDLVRSELDKPRSRKKTPVTDSEEKLGMDDLTKLVQEQKPKGSQRGRKRGHTASESDEQQWSEEKRLKEDILENEDEQNSPPKKGKRGRPPKPLGGGTPKEEPTIKTSKKGSKKKSGPSAAEEEEEEEKQSGNTEQKSKSKQHRTSRRAQQRAESPETSAVESTQSTPQKGRGRPSKTPSPSQPKKNVRVGRSKQAATKENDSSEEVDVFQGSSPVNDDIPQEETEEEEVSTVNVCVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KFN1</accession>
    <name>A0A9L0KFN1_EQUAS</name>
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        <recommendedName>
            <fullName evidence="3">Protein phosphatase 4 regulatory subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">LOC123280820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0KFN1"/>
    <dbReference type="Ensembl" id="ENSEAST00005076675.1">
        <property type="protein sequence ID" value="ENSEASP00005061432.1"/>
        <property type="gene ID" value="ENSEASG00005025372.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00960000192205"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008287">
        <property type="term" value="C:protein serine/threonine phosphatase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039918">
        <property type="entry name" value="PPP4R4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21467">
        <property type="entry name" value="PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 4 PPP4R4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21467:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="63" mass="7262" checksum="1ABAB3818FDA2867" modified="2023-09-13" version="1">MHPPPPGAAMDFSQNSLFGYMEDLQELTIIERPVRRSLKTPEEIERLTVDEDLSDTERAVYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KGU0</accession>
    <name>A0A9L0KGU0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106842280</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cell membrane</location>
            <topology evidence="2 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014714179.2">
        <property type="nucleotide sequence ID" value="XM_014858693.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0KGU0"/>
    <dbReference type="Ensembl" id="ENSEAST00005066459.1">
        <property type="protein sequence ID" value="ENSEASP00005060705.1"/>
        <property type="gene ID" value="ENSEASG00005035512.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106842280"/>
    <dbReference type="KEGG" id="eai:106842280"/>
    <dbReference type="GeneTree" id="ENSGT00940000153255"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15420">
        <property type="entry name" value="7tmA_OR2A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000008">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="57"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="236"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="40"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005060705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="310" mass="34942" checksum="51D48C2B295822C9" modified="2023-09-13" version="1">MGSNQSWVTEFVLVGFELSADVEVLLLWVFSLFYIFSLLANGVILGLICLDPSLHTPMYFFLSHLAIIDMSYASSNVPKMLANLVKHNRTISFVPCIMQTFLYLGFAATECLILVVMSYDRFVAICHPLQYTVIMNWRVCTVLAITSWSCGFILALVHAILLLRLPFCGSREVNHLFCEILSVLKLACVDTWINQVVILAASVFVLVGPLCLMLVSYMRIVWAILKIQSKEGRTKAFSTCSSHLCVVGLFFGIAMVVYMVPDSNQQEEQEKILSLFHSLFNPMLNPLIYSLRNAQVKGALYRALQKKRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KT79</accession>
    <name>A0A8C4KT79_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Shortage in chiasmata 1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KT79"/>
    <dbReference type="Ensembl" id="ENSEAST00005001257.2">
        <property type="protein sequence ID" value="ENSEASP00005001102.2"/>
        <property type="gene ID" value="ENSEASG00005000898.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013037"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039991">
        <property type="entry name" value="SHOC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35668">
        <property type="entry name" value="PROTEIN SHORTAGE IN CHIASMATA 1 ORTHOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35668:SF1">
        <property type="entry name" value="PROTEIN SHORTAGE IN CHIASMATA 1 ORTHOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17825">
        <property type="entry name" value="DUF5587"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="705"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="705"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="717"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001102.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1040" mass="118496" checksum="697F4CB007B5E64C" modified="2023-09-13" version="2">MYQAQLEYESCSSPIVLIKEKSANDHLSLPRKSASVADLCFSDEDISVKNLMREEIPKNDLELVHRIIQNEENNDCLELGCTVPSIESSSPSKMEKASFKNGEKQENDLDLLSDFIMLRNKYRTCTSNTEVTDSDEKNEFRDKEEHSLTLQEESPIVCTDKTLEKINQERRADNVIEIQASDSQCQAYCLLEAAATPIFKKLACLCTLPVANWEFATVVFDQTRFLLKEQQKVISDAIHQGTNDEREMTFKHAALLHLLVTIRDLLLTCSLDTALGYLSNAKDIYKSILGSYLDDIWRQLAIVQFIREKNPETNYKIQELQCQILNWMQSEQQIQVLIIIRMDSDGEKHSLIKILDKMEGLTLTTLHSNRRKDFLESEAVLNGLSSCVVVHNQYIGADFPWSRFSFVVEYNYVENSCWTKHCEKLNIPYMAFKAILPDTVVKSSTLLDRFGGFLLEIQIPYVFFASEGLLNTPEILQVLESNYNITLVERCCSEALKLFGGTERYVVVTIDEHTAVILQDLEELNCEKASDNVITRLMALSFQYSYCWIILYTKETLNSEYHLTEKTLHHLALIYAALGSFGLKPEELDVKLIIAPGVEETALIIRQIADHNLMTSKRDPYEWLDKSWLEVSPSKEEMYLLDFPCINPLVAQLMLNKGPSLDWILLATLGQLQELLPEVPEKVLKHFCGITSLFKINSSSVRKSPQISSLQENGNQTSTFTSQTSASGSSDSVIQEHNKYDQYSDLGETVHDDTNTASNDNSSLMALRDMPCISPPVASYNQTSYWKDSSCNPNMVQNNPFLINRESRNVAGNSFLNQNGSESDVFSLGLTQMNGETIISPIDTQRRLTSNFINYPKKGTHEAKGHINKEVSAPLFSLEGSQSPLHWNFKENVWEQQNHSFNLQYGAERTTCGKWYSQKDNLFTNQQRCLSDELDSFIRESSHTGTKNTFWRELPSVPSLDSFCASDSNVNPKEFNSLYFYQRAGKCLGQKRHLESSSDSGDKKSLTDFMCSQLPQFKKRRLVYEKIPGRADGQTRLRFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KU40</accession>
    <name>A0A8C4KU40_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">T-cell surface glycoprotein CD8 alpha chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CD8A</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="15">Forms disulfide-linked heterodimers with CD8B at the cell surface. Also forms homodimers in several cell types including NK-cells or peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KU40"/>
    <dbReference type="Ensembl" id="ENSEAST00005002423.2">
        <property type="protein sequence ID" value="ENSEASP00005002197.1"/>
        <property type="gene ID" value="ENSEASG00005001721.2"/>
    </dbReference>
    <dbReference type="KEGG" id="eai:106838579"/>
    <dbReference type="GeneTree" id="ENSGT00940000156588"/>
    <dbReference type="OMA" id="KCKCIRP"/>
    <dbReference type="OrthoDB" id="9906515at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044853">
        <property type="term" value="C:plasma membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023024">
        <property type="term" value="F:MHC class I protein complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045065">
        <property type="term" value="P:cytotoxic T cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002456">
        <property type="term" value="P:T cell mediated immunity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000956">
        <property type="entry name" value="T-cell surface glycoprotein CD8 alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015468">
        <property type="entry name" value="CD8_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10441">
        <property type="entry name" value="CD8 ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10441:SF2">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD8 ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0564">Palmitate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 18" id="KW-0732">Signal</keyword>
    <keyword evidence="4 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034088074" description="T-cell surface glycoprotein CD8 alpha chain" evidence="18">
        <location>
            <begin position="22"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="11"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="143"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="144"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
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    <evidence type="ECO:0000256" key="11">
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    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005002197.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="244" mass="26990" checksum="80986D226B0D0F7B" modified="2022-01-19" version="1">MAEPGTFLLLPLALLLHAVLALGSNAFRMTQPEGPPQPGKTLNLRCQVLLSNQAPGCSWLYQPPGPAARPVFLMYITKGRIKPAEDLDTKKFSGERIQDAVFGLTLHHFSEKDQGYYFCSVLSNSIIYFSPFVPVFLPAKPTTTPAPRPPTPRPPMRAATNASQPVTRRPETCRPAKGSPVGKKWLDFDCDIYIWAPLAGTCAVLLLSLIITIICNHRNRRRVCKCPRPLVRPAGKPTTSERYV</sequence>
</entry>
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            <fullName evidence="10">Ig-like domain-containing protein</fullName>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Eutheria</taxon>
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            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
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                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L336"/>
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        <property type="gene ID" value="ENSEASG00005003469.2"/>
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        <property type="component" value="Chromosome 25"/>
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        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001916">
        <property type="term" value="P:positive regulation of T cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21029">
        <property type="entry name" value="IgC1_CD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000254">
        <property type="entry name" value="Antigen-presenting glycoprotein CD1d1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.500.10:FF:000002">
        <property type="entry name" value="Antigen-presenting glycoprotein CD1d1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF160">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16497">
        <property type="entry name" value="MHC_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG_LIKE"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040320137" description="Ig-like domain-containing protein" evidence="9">
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            <begin position="19"/>
            <end position="339"/>
        </location>
    </feature>
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            <begin position="301"/>
            <end position="322"/>
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    </feature>
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        <location>
            <begin position="185"/>
            <end position="294"/>
        </location>
    </feature>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="339" mass="38426" checksum="C741BB41A66BC4AF" modified="2023-09-13" version="2">MWFLQLSLLVILLPGCDNKDGFQEPISFEIIRIHTFYSRSWVQTLGSIWVDELQTHKWDSNSGNYIFLWPWSKGNFSNEEWIEQEKLCSTFSIQLIRAFQNRASQWQLEYPFEVQTTAGYELRFGETSVGFIRVAYQGSDFISFQNTSWLPSPQGGSRAQDACRLFNLNQVGLEIADRFLSDTCPRLLLGILDAGKADLQRQVKPEAWLSTGPSSGPGHLTLVCHVSGFHPKPIWVMWMRGEQEHPGTQRSDILPNADGTWYLRKSLDVEAIEAAGLSCRVRHSSLGGQDIILSWENDSSMGLIFAAVIVSLVLLTGLAFWLRKRRKPCATPRTPLPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L6D2</accession>
    <name>A0A8C4L6D2_EQUAS</name>
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        <recommendedName>
            <fullName evidence="6">rRNA biogenesis protein RRP36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RRP36</name>
    </gene>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
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                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the 90S pre-ribosome involved in the maturation of rRNAs. Required for early cleavages of the pre-RNAs in the 40S ribosomal subunit maturation pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Associates with 90S and pre-40S pre-ribosomal particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Nucleus</location>
            <location evidence="1 6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the RRP36 family.</text>
    </comment>
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    <keyword evidence="4 6" id="KW-0698">rRNA processing</keyword>
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            <begin position="225"/>
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        <location>
            <begin position="232"/>
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        </location>
    </feature>
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    <sequence length="251" mass="28956" checksum="0073F2463AEEE269" modified="2023-09-13" version="2">MPRASARSVAAAGFPRGARDGGEDCGSPGPGDLQKGTSNMSFEELLELQNQVGTKTYKQLVAGKSTKKQGSRPPVQNACVADKHRPLEMSAKVRVPFLRQVVPISKKVARDPRFDDLSGEYNPEVFDKTYQFLNDIRAKEKELVKKQLKKHRSGEEHEKLQQLLHRMEQQEMAQQERRRQQELHLALKQQRRAQAQQGHRPYFLKKSEQRQLALAEKFKELKRSKKLESFLSRKRRRNAGKDRRHLPLGKE</sequence>
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    <accession>A0A8C4L6D4</accession>
    <name>A0A8C4L6D4_EQUAS</name>
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            <fullName evidence="14">Protein S100</fullName>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Facilitates the differentiation and the cornification of keratinocytes.</text>
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        <text evidence="5">Homodimer; disulfide-linked.</text>
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    <comment type="subcellular location">
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        </subcellularLocation>
        <subcellularLocation>
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    <comment type="similarity">
        <text evidence="4 14">Belongs to the S-100 family.</text>
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        <property type="term" value="F:calcium ion binding"/>
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    <dbReference type="GO" id="GO:0048306">
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    <dbReference type="GO" id="GO:0044548">
        <property type="term" value="F:S100 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
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    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
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    <dbReference type="InterPro" id="IPR001751">
        <property type="entry name" value="S100/CaBP7/8-like_CS"/>
    </dbReference>
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        <property type="entry name" value="S100_Ca-bd_sub"/>
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    <dbReference type="InterPro" id="IPR028482">
        <property type="entry name" value="S100A11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639:SF60">
        <property type="entry name" value="PROTEIN S100-A11"/>
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    </dbReference>
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        <property type="entry name" value="S100 CALCIUM-BINDING PROTEIN"/>
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        <property type="entry name" value="S100_CABP"/>
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    <keyword evidence="8 14" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
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            <begin position="55"/>
            <end position="90"/>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="105" mass="11700" checksum="E153D73894F162A7" modified="2023-09-13" version="2">AAKMSSPTETERCIESLIAVFQRYAGKDGNSSKLSKTEFLTFMNRELAAFTKNQKDPGVLDRMMKKLDLNADGQLDFQEFLNLIGGLAIACHESFLKSAHSQKIL</sequence>
</entry>
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    <accession>A0A8C4L7K0</accession>
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        <submittedName>
            <fullName evidence="2">Transmembrane protein 74B</fullName>
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        <name evidence="2" type="primary">TMEM74B</name>
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            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
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                <person name="J."/>
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                <person name="A."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_044748244.2"/>
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        <property type="nucleotide sequence ID" value="XM_044748245.2"/>
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    <dbReference type="GeneTree" id="ENSGT00530000063880"/>
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        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029695">
        <property type="entry name" value="TMEM74-like"/>
    </dbReference>
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        <property type="entry name" value="TRANSMEMBRANE PROTEIN 74"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR16125:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 74B"/>
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        <property type="entry name" value="Neurensin"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="84"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005004728.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
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    <accession>A0A8C4L8Q8</accession>
    <name>A0A8C4L8Q8_EQUAS</name>
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        <submittedName>
            <fullName evidence="17">Solute carrier family 17 member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SLC17A3</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>3 Na(+)(out) + phosphate(out) = 3 Na(+)(in) + phosphate(in)</text>
            <dbReference type="Rhea" id="RHEA:71255"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Sodium/anion cotransporter family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014851580.3"/>
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    <dbReference type="GeneTree" id="ENSGT00940000162523"/>
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        <property type="component" value="Chromosome 8"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:perinuclear region of cytoplasm"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008514">
        <property type="term" value="F:organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019534">
        <property type="term" value="F:toxin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015143">
        <property type="term" value="F:urate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008308">
        <property type="term" value="F:voltage-gated monoatomic anion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046415">
        <property type="term" value="P:urate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990961">
        <property type="term" value="P:xenobiotic detoxification by transmembrane export across the plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd17318">
        <property type="entry name" value="MFS_SLC17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000003">
        <property type="entry name" value="Solute carrier family 17 member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000060">
        <property type="entry name" value="Solute carrier family 17 member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050382">
        <property type="entry name" value="MFS_Na/Anion_cotransporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662:SF134">
        <property type="entry name" value="SODIUM-DEPENDENT PHOSPHATE TRANSPORT PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11662">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="337"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="371"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="395"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="429"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="464"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="16">
        <location>
            <begin position="41"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00008586"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00035839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007365.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="499" mass="54527" checksum="CA4F14D1881EDFF7" modified="2023-09-13" version="2">MATMTELSSTEEKNKYSQDTQVDEKLILRKAPSLCSTRYGIAFLAHLCNFTIMAQNVIMNISMVTMVNSTGHQSQFNNSTEGPPVDSFGGPNNAPKSLPAGKAPVYDWSPQIQGIIFGSINYGMLLTLVPGGYLAGRVGTKRVLGFSLFGSSILSLFTPLAADLGLVFLIATRLLQGLTQGSVFGGQFALWERWGPPHERSRLCSFALSGMIMGAFTAILLGGVISQALGWPFVFYIFGGIGCVCCLLWFVLVYDDPVSHPWINVTEKEYIISSLAQQVSSSKQSLPIKAVVRSLPFWSICFCSFSHLWLINIFIVYTPTYISSVFHVNIRDNGFLSALPFIVAWVIGVLGGHLADFLLTKNFRIVTVRKIATVLGILPPSAFLVALPYLTSSYITTMTLLTLACGLSSLCQTGVYINALDIAPRYSSFLTGAARGFAQIAAVLAPTVSGFLLSQDSEFGWRNVFLLMFAINLLGLLIYLIFGEGDVQDWAKERKLTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L922</accession>
    <name>A0A8C4L922_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Mitochondrial fission regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MTFR1L</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Mitochondrial protein required for adaptation of miochondrial dynamics to metabolic changes. Regulates mitochondrial morphology at steady state and mediates AMPK-dependent stress-induced mitochondrial fragmentation via the control of OPA1 levels.</text>
    </comment>
    <comment type="function">
        <text evidence="7">Plays a role in mitochondrial aerobic respiration. Regulates mitochondrial organization and fission.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the MTFR1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014710504.1">
        <property type="nucleotide sequence ID" value="XM_014855018.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014710505.1">
        <property type="nucleotide sequence ID" value="XM_014855019.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044625970.1">
        <property type="nucleotide sequence ID" value="XM_044770035.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044625971.1">
        <property type="nucleotide sequence ID" value="XM_044770036.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070366757.1">
        <property type="nucleotide sequence ID" value="XM_070510656.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070366758.1">
        <property type="nucleotide sequence ID" value="XM_070510657.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070366759.1">
        <property type="nucleotide sequence ID" value="XM_070510658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L922"/>
    <dbReference type="Ensembl" id="ENSEAST00005006158.2">
        <property type="protein sequence ID" value="ENSEASP00005005636.1"/>
        <property type="gene ID" value="ENSEASG00005004168.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106839939"/>
    <dbReference type="KEGG" id="eai:106839939"/>
    <dbReference type="CTD" id="56181"/>
    <dbReference type="GeneTree" id="ENSGT00950000183215"/>
    <dbReference type="OMA" id="LRHKWKP"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009060">
        <property type="term" value="P:aerobic respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007972">
        <property type="entry name" value="Mtfr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14215:SF3">
        <property type="entry name" value="MITOCHONDRIAL FISSION REGULATOR 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14215">
        <property type="entry name" value="PROTEIN OF UNKNOWN FUNCTION DUF729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05308">
        <property type="entry name" value="Mito_fiss_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="292" mass="32098" checksum="0DA0EF65CA05EE80" modified="2022-01-19" version="1">MSEMEANVTIPIWQNKPHGAARSVVRRIGTNLPLKPCPRASFETLPNISDLCLRDVPPVPTLADIAWIAADEEETYARVRSDTRPLRHTWKPSPLIVMQRNASVPNLRGSEERLLALKKPALPALSRTTELQDELSHLRSQIAKIVAGDAASASLTPDFLSPGSSNVSSPLPCFGSSFHSTTSFVISDITEENEVEVPELPSVPLLCSASPECCKPEHKATCSSSEEDDCVSLSKASSFADMMGILKDFHRMKQSQDLNRSLLKEEDPAVLISEVLRRKFALKEEDISRKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L9L7</accession>
    <name>A0A8C4L9L7_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="23">STEAP3 metalloreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">STEAP3</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>2 Cu(+) + NADP(+) + H(+) = 2 Cu(2+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:71771"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
            <dbReference type="ChEBI" id="CHEBI:49552"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:71773"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>2 Fe(2+) + NADP(+) + H(+) = 2 Fe(3+) + NADPH</text>
            <dbReference type="Rhea" id="RHEA:71767"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:71769"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endosome membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the STEAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L9L7"/>
    <dbReference type="Ensembl" id="ENSEAST00005005180.2">
        <property type="protein sequence ID" value="ENSEASP00005004727.2"/>
        <property type="gene ID" value="ENSEASG00005003559.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008042"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008823">
        <property type="term" value="F:cupric reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000051">
        <property type="entry name" value="STEAP2 metalloreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051267">
        <property type="entry name" value="STEAP_metalloreductase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239">
        <property type="entry name" value="DUDULIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239:SF8">
        <property type="entry name" value="METALLOREDUCTASE STEAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="11" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="16" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0410">Iron transport</keyword>
    <keyword evidence="17 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="252"/>
            <end position="280"/>
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    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="305"/>
            <end position="326"/>
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    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="359"/>
            <end position="379"/>
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    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="391"/>
            <end position="412"/>
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    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="432"/>
            <end position="454"/>
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    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="22">
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            <begin position="30"/>
            <end position="117"/>
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    <feature type="domain" description="Ferric oxidoreductase" evidence="21">
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            <begin position="261"/>
            <end position="405"/>
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            <dbReference type="ARBA" id="ARBA00004337"/>
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    <evidence type="ECO:0000256" key="4">
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            <dbReference type="ARBA" id="ARBA00007729"/>
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    <evidence type="ECO:0000256" key="5">
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            <dbReference type="ARBA" id="ARBA00049387"/>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="488" mass="54386" checksum="1482A24D826041B6" modified="2023-09-13" version="2">TSGEMDKPLISRRLVDSDGSLAEAPSEAPKVGILGSGDFARSLTTRLVGSGFGVVVGSRNPKRMAGLFPSAAQVTFQEEAVGSPEVIFVAMFREHYSSLCSLSSQLAGKILVDVSNPTEQEHLQHRESNAEYLASLFPTCSVVKAFNVISAWTLQSGPRDGNRQVPICSDQPEAKHTVSEMVHAMGFTPVDMGSLVSAREVEAMPLRLLPGWKVPALLTLGLLVFFYAYNFVRDVLHPYLQEGKNKFYKLPVSVVNTTLPCVAYVLLSLVYLPGVLAAALQLRRGTKYRRFPDWLDHWLQHRKQIGLLSFFCAALHALYSFCLPLRRSHRYDLVNLAVKQVLANKSHLWVEEEVWRMEIYVSLGVLALGTLSLLAVTSLPSIANSLNWREFSFVQSTLGFVALVLSTLHTLTYGWTRAFEESRYKFYLPPTFTLTLLVPCVVILARGLFLLPCVSRRLSKIRRGWEKDGAIKFTLPMDHTLAQKTSHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LAT7</accession>
    <name>A0A8C4LAT7_EQUAS</name>
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        <recommendedName>
            <fullName evidence="9">Protein Dok-7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Downstream of tyrosine kinase 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">DOK7</name>
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    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Forms a heterotetramer composed of 2 DOK7 and 2 MUSK molecules which facilitates MUSK trans-autophosphorylation on tyrosine residue and activation. Interacts (via IRS-type PTB domain) with MUSK (via cytoplasmic part); requires MUSK phosphorylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Synapse</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_044767908.2"/>
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        <property type="protein sequence ID" value="ENSEASP00005006000.2"/>
        <property type="gene ID" value="ENSEASG00005004429.2"/>
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    <dbReference type="GeneTree" id="ENSGT00390000015386"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:neuromuscular junction"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007167">
        <property type="term" value="P:enzyme-linked receptor protein signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007528">
        <property type="term" value="P:neuromuscular junction development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099645">
        <property type="term" value="P:neurotransmitter receptor localization to postsynaptic specialization membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035022">
        <property type="term" value="P:positive regulation of Rac protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904395">
        <property type="term" value="P:positive regulation of skeletal muscle acetylcholine-gated channel clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016601">
        <property type="term" value="P:Rac protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14677">
        <property type="entry name" value="PH_DOK7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13165">
        <property type="entry name" value="PTB_DOK7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Docking protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="protein Dok-7 isoform X2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037746">
        <property type="entry name" value="Dok-7"/>
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    <dbReference type="InterPro" id="IPR037747">
        <property type="entry name" value="Dok-7_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037748">
        <property type="entry name" value="Dok-7_PTB"/>
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    <dbReference type="InterPro" id="IPR002404">
        <property type="entry name" value="IRS_PTB"/>
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    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21636">
        <property type="entry name" value="PROTEIN DOK-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21636:SF2">
        <property type="entry name" value="PROTEIN DOK-7"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF02174">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01244">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51064">
        <property type="entry name" value="IRS_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="PH" evidence="12">
        <location>
            <begin position="4"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="IRS-type PTB" evidence="13">
        <location>
            <begin position="105"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="250"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="367"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="500"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="572"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="263"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="285"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="458"/>
            <end position="478"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="583"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055944"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00063999"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071504"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00077462"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006000.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="601" mass="62884" checksum="CF95024B14EA55D7" modified="2023-09-13" version="2">MTEAALVEGQVKLRDGKKWKSRWLVLRKPSPVADCLLMLAYKDKSERAKGLRERSSLTLEDICGLEPGLPYEGLAHTLAIICLSQAVMLGFDSREAMCAWDARIRYALGEVHRFHVTVAPGTKLESGPATLHLCNDILVVARDVPPAVTGQWKLSDLRRYGAVPNGFIFEGGTRCGYWAGVFFLSSAEGEQISFLFDCIVRGISPTKGPFGLRPVLPDPSPGGPATVEERVAQEALEALQLEKRLSLLSHSCRPGSAGDDRSLSSSSSEASHSDVSAGSRLTAWPEQSSSSAGTSQEGPGMAAAQAPGEAALGASRPPPKPLRPRQLQEVGRQSSSDSGIATGSHSSYSGSFSSCAGSSLDVWRAGDDFGSLLSLPPMPGAPEPSLCACPPGAAEYQVPGVPRPHYDTPRSLRHGPRDQPPATQGRPGDAAAGDLGSQTSAGCPSGWLGARRRGQATEAPGGEAALPSPAPSEPWEAGVSPAGPPAAFFSVCPVCGGLKGAAASPPGLPMTHSGALGPESPRSEAPTYVNIPASPSSKKQLHYMGLELQEASTGVRGAGTSRYAQIDITATEAAHRAGARHAQSREERLPELEQRRKGAPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LDE2</accession>
    <name>A0A8C4LDE2_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ankyrin repeat and kinase domain containing 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">ANKK1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LDE2"/>
    <dbReference type="Ensembl" id="ENSEAST00005010257.2">
        <property type="protein sequence ID" value="ENSEASP00005009439.2"/>
        <property type="gene ID" value="ENSEASG00005006737.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162060"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010564">
        <property type="term" value="P:regulation of cell cycle process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF175">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Protein kinase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="306"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="339"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="372"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="405"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="438"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="471"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="504"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="537"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="570"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="603"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="636"/>
            <end position="668"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009439.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="710" mass="78498" checksum="9A66F844A21EEFFB" modified="2023-09-13" version="2">MEKIKFQHIVSIYGVCRKPLGIVMEFMANGSLEKMLSTHCLCWQLKFRIIHETSLAMNFLHSIQPPLLHLDLKPGNILLDSNMHVKISDFGLSKWMEQSTRMQYIERSALRGTLSYIPPEMFVETNKAPGPKYDVYSFGIVIWELLTQQKPYSGFSMMTIIIRVATGGRPSLQPVSEEWPGEAQQMVDLMKRCWDQDPKKRPCFPDITVETDMLLSLLQSTVADPESEALARKVSCKPSLCRSWEVRAVAGHGLWHVLRMRAQVSVEVNQELTDIGNSGDYLKRVLQLSDSGSLVPSDEELRIYENKVTPLHFLVAQGSMEQVKLLLAHEVDVDCQTACGYTPLLIAAQDQQPDLCTLLLEHGANANLADEDDWAPLHFAAQNGDDRTARLLLDHGAHVDAQEHEGWTPLHLAAQNNFENVARLLVSRQADPNLHEAEGKTPLHVAAYFGHVNLVKLLTGQGAELDARQRNLRTPLHLAVERGKVRAIQHLLKSGAAPNALDQRSYSPLHIAAARGKYLVCKMLLRYGASLELPTQQGWTPLHLAAYKGHLEIIRLLAESHADLGAPGGMNWTPLHLAARHGAEVVVSALLQCGADPNAAEQSGWTPLHLAVQRGTFLSVVNLLEHRADVHARNKVGWTPAHLAALQGNMAILKVLVKAGAQLDIQDGMGCTPLQLALRSQKQGIVPFLEGKEPLLAVLGSDESGAQMDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LJ82</accession>
    <name>A0A8C4LJ82_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Exosome complex component RRP4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Exosome component 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Ribosomal RNA-processing protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">EXOSC2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Component of the RNA exosome core complex (Exo-9), composed of EXOSC1, EXOSC2, EXOSC3, EXOSC4, EXOSC5, EXOSC6, EXOSC7, EXOSC8 and EXOSC9; within the complex interacts with EXOSC4 and EXOSC7. The catalytically inactive RNA exosome core complex (Exo-9) associates with the catalytic subunit EXOSC10/RRP6. Exo-9 may associate with DIS3 to form the nucleolar exosome complex, or DIS3L to form the cytoplasmic exosome complex. Exo-9 is formed by a hexameric base ring consisting of the heterodimers EXOSC4-EXOSC9, EXOSC5-EXOSC8 and EXOSC6-EXOSC7, and a cap ring consisting of EXOSC1, EXOSC2 and EXOSC3. The RNA exosome complex associates with cofactors C1D/RRP47, MPHOSPH6/MPP6 and MTREX/MTR4. Interacts with GTPBP1. Interacts with ZFP36L1 (via N-terminus).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRP4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014718939.1">
        <property type="nucleotide sequence ID" value="XM_014863453.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LJ82"/>
    <dbReference type="Ensembl" id="ENSEAST00005013189.2">
        <property type="protein sequence ID" value="ENSEASP00005012141.2"/>
        <property type="gene ID" value="ENSEASG00005008475.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106845391"/>
    <dbReference type="KEGG" id="eai:106845391"/>
    <dbReference type="CTD" id="23404"/>
    <dbReference type="GeneTree" id="ENSGT00940000153596"/>
    <dbReference type="OrthoDB" id="1650at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071034">
        <property type="term" value="P:CUT catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22525">
        <property type="entry name" value="KH-I_Rrp4_eukar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05789">
        <property type="entry name" value="S1_Rrp4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000022">
        <property type="entry name" value="Exosome complex component RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000038">
        <property type="entry name" value="Exosome complex component RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025721">
        <property type="entry name" value="Exosome_cplx_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026699">
        <property type="entry name" value="Exosome_RNA_bind1/RRP40/RRP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048565">
        <property type="entry name" value="S1_RRP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321:SF4">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21321">
        <property type="entry name" value="PNAS-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14382">
        <property type="entry name" value="ECR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15985">
        <property type="entry name" value="KH_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21266">
        <property type="entry name" value="S1_RRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110324">
        <property type="entry name" value="Ribosomal L27 protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exosome complex component N-terminal" evidence="14">
        <location>
            <begin position="26"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="RRP4 S1" evidence="16">
        <location>
            <begin position="75"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="15">
        <location>
            <begin position="169"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00071123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF14382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF15985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF21266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005012141.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="293" mass="32644" checksum="40ECC81838139D7A" modified="2023-09-13" version="2">MAMEMRLPVARKPLSESLGRESKKHLVVPGDTITTDTGFMRGHGTYMGEEKLIASVAGSVERVNKLICVKALKTRYNGEVGDIVVGRITEVQQKRWKVETNSRLDSVLLLSSMNLPGGELRRRSAEDELAMRGFLQEGDLISAEVQAVFSDGAVSLHTRSLKYGKLGQGVLVQVSPSLVKRQKTHFHDLPCGASVILGNNGFIWIYPTPEHKEEDAGGFIANLEPVSLADREVISRLRNCIVSLVTQRMMLYDTSILYCYEASLPHPIKDILKPEIMEEIVLETRQRLLEQEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LKM8</accession>
    <name>A0A8C4LKM8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Gamma-tubulin complex component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TUBGCP5</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation. The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the gamma-tubulin ring complex (gTuRC) consisting of TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6 and gamma-tubulin TUBG1 or TUBG2. TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6 assemble in a 5:5:2:1:1 stoichiometry; each is associated with a gamma-tubulin, thereby arranging 14 gamma-tubulins in a helical manner. Gamma-tubulin at the first position is blocked by TUBGCP3 at the last position, allowing 13 protafilaments to grow into a microtubule. The gTuRC (via TUBGCP3 and TUBGCP6) interacts with ACTB and MZT1; the interactions form a luminal bridge that stabilizes the initial structure during complex assembly. The gTuRC (via TUBGCP2) interacts with MZT2A/MZT2B and CDK5RAP2 (via CM1 motif); the interactions play a role in gTuRC activation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070353226.1">
        <property type="nucleotide sequence ID" value="XM_070497125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LKM8"/>
    <dbReference type="Ensembl" id="ENSEAST00005011744.2">
        <property type="protein sequence ID" value="ENSEASP00005010801.2"/>
        <property type="gene ID" value="ENSEASG00005007587.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106823935"/>
    <dbReference type="CTD" id="114791"/>
    <dbReference type="GeneTree" id="ENSGT00940000155962"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051011">
        <property type="term" value="F:microtubule minus-end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22572">
        <property type="entry name" value="GCP5_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1900:FF:000005">
        <property type="entry name" value="Gamma-tubulin complex component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059169">
        <property type="entry name" value="GCP5_N_ext"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF33">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="11">
        <location>
            <begin position="272"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="10">
        <location>
            <begin position="717"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="152"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="522"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="532"/>
            <end position="544"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00093416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005010801.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1025" mass="118263" checksum="9AFA2D42C0FB66BC" modified="2023-09-13" version="2">MARPCLLWSRLDRQQEQDVRELVRRVAGLRDEADPDFQLALHFVLSNFRFHHFLDVNSHKVEKTIEGIYEKFIIHSDLSKAASWKRLTEEFLNASLPSIKEIKTDAHYSILSLLLCLSDSPSNSNYVETPRDKEVEKKDDFDWGKYLMEGEEIDLGPNADTPNWSEESEGEDDQQPLSREDSGIQVDRTPLEAQDQNRKPGPSVNWKVDEPDARSWLEQHVIRQYWTARSSRFPHSLHLHSNLAAVWDQHLYSSDPLYVPDDRVFVTEMQVIRETLWLLSGVKKLFIFQLIDGKVTVRNNIIVTHLTHSCLRSVLEQIAAYGQVVFRLQEFIDEVMGHSSESMLPGNGSIPKKSTEPPFRTYQAFMWALYKYFISFKEELTEIEKCIISNDTTITLAIVVDKLSPRLAQLKVLHKVFSTGVAEVPPDTRNVVRASHLLNTLYKAILEYDNVGEASEQTVSLLFSLWVETVRPYLQTVDEWIAHGHLCDCAREFIIQRNKNVPVNHRDFWYATYTLYSVSEKTENEEKMSDNASASSGSDQGPSSRQHTMVSFLKPVLKQIIMAGKSMQLLKNLQCAESTTCQATARDAERKSLYTLFLESVQSRLRHGEEPTAQVLTEQQETKGNLIKIQSIAERHLELDDVHDPLLAINFARLYLEQSDFHEKFAGGDICVDRSSESVTCQTFELTLRSCLYPHIDKQYLDCCGNLMQTLKKDYRLVEYLQAMRNFFLMEGGDTMYDFYTSIFDKIREKETWQNVSFLNVQLQEAVGQRYPEDSSRLSITFENVDTAKKKLPVHILDGLTLSYKVPWPVDIVISLECQKIYNQVFLLLLQIKWAKYSLDVLLFGELASTAEKPQLKEEVLCEQDTAAQFGPQKEPVRQQIHRMFLLRVKLMHFVNSLHNYIMTRILHSTGLEFQHQVEEAKDLDQLIKIHYRYLSTIHDRCLLREKVSFVKEAIMKVLNLALMFAEGWQAGLGAWQMESIEKMESDFKNCHMFLVTILNKAVCRGSFPHLESLALSLMAGMEQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LLV2</accession>
    <accession>A0A8C4MP84</accession>
    <name>A0A8C4LLV2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Protein BEX1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106823046</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the BEX family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014683987.1">
        <property type="nucleotide sequence ID" value="XM_014828501.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LLV2"/>
    <dbReference type="Ensembl" id="ENSEAST00005011497.2">
        <property type="protein sequence ID" value="ENSEASP00005010563.2"/>
        <property type="gene ID" value="ENSEASG00005019756.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005031537.2">
        <property type="protein sequence ID" value="ENSEASP00005029021.2"/>
        <property type="gene ID" value="ENSEASG00005019756.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005073014.1">
        <property type="protein sequence ID" value="ENSEASP00005040555.1"/>
        <property type="gene ID" value="ENSEASG00005019756.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106823046"/>
    <dbReference type="KEGG" id="eai:106823046"/>
    <dbReference type="GeneTree" id="ENSGT00940000153412"/>
    <dbReference type="OrthoDB" id="9833968at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007623">
        <property type="entry name" value="BEX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021156">
        <property type="entry name" value="TF_A-like/BEX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19430:SF3">
        <property type="entry name" value="PROTEIN BEX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19430">
        <property type="entry name" value="PROTEIN BEX1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04538">
        <property type="entry name" value="BEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008633">
        <property type="entry name" value="BEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005010563.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="15300" checksum="2B80DE66882E13C1" modified="2023-09-13" version="2">MESKEEQVVKNIDMKNTNQENEKKDEKEQDANKGEHLALPLETGECCVPRGNRGQFHVRQSILQYRWDMIQRLEEPQARMREENMERIREEVRQLREKLGEKQLSHCLRAVSTDPPHHDHHDEFCLMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LLZ4</accession>
    <name>A0A8C4LLZ4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">tissue kallikrein</fullName>
            <ecNumber evidence="8">3.4.21.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">KLK7</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.35"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family. Snake venom subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.35" evidence="8"/>
    <dbReference type="RefSeq" id="XP_014722410.1">
        <property type="nucleotide sequence ID" value="XM_014866924.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LLZ4"/>
    <dbReference type="Ensembl" id="ENSEAST00005015213.2">
        <property type="protein sequence ID" value="ENSEASP00005014004.1"/>
        <property type="gene ID" value="ENSEASG00005009761.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106847578"/>
    <dbReference type="KEGG" id="eai:106847578"/>
    <dbReference type="CTD" id="5650"/>
    <dbReference type="GeneTree" id="ENSGT01020000230389"/>
    <dbReference type="OMA" id="QECSKVY"/>
    <dbReference type="OrthoDB" id="10061449at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001533">
        <property type="term" value="C:cornified envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097209">
        <property type="term" value="C:epidermal lamellar body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002803">
        <property type="term" value="P:positive regulation of antibacterial peptide production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000021">
        <property type="entry name" value="Kallikrein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000010">
        <property type="entry name" value="Kallikrein related peptidase 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF45">
        <property type="entry name" value="KALLIKREIN-7"/>
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    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0720">Serine protease</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
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        <location>
            <begin position="1"/>
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        </location>
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        <location>
            <begin position="23"/>
            <end position="257"/>
        </location>
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    <feature type="domain" description="Peptidase S1" evidence="11">
        <location>
            <begin position="34"/>
            <end position="254"/>
        </location>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    <name>A0A8C4LPI1_EQUAS</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="J."/>
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                <person name="J."/>
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                <person name="X."/>
                <person name="Zhao"/>
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                <person name="Liu"/>
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                <person name="Wang"/>
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                <person name="Wang"/>
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                <person name="Li"/>
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                <person name="Zhang"/>
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                <person name="J."/>
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                <person name="J."/>
                <person name="Liu"/>
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                <person name="Wu"/>
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                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <location>
            <begin position="137"/>
            <end position="157"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014945.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="299" mass="32161" checksum="B3ABF4A57059CA99" modified="2023-09-13" version="2">MPIRKGPYQYRNPVIIQRWCCVLHDPDTVGEEEETVLTHAPHDQKFAQEEEEVGNFVEDRHSAGEGEKQEEGVPGRGAEVGAVDGNLQGGIPVEEFDKLLQTPEAAFKTGHEELGKPACTFQALIQVLQHHSGDLHNGQNEGAKGQGACVRPAEGGEEREGRQVVWLVEGPVVGGEGPSQRDLAQGQHEVGQPEEHEDIEDLQAQQGPVVARLPAIEGELAVGAGAQVGFVGGGESLREGRRAGSQHRGQGFRVALGIKAGSGRPWCGTGVMGIRIKSRDWGFKIGAYKRGSRSNGRDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LPI4</accession>
    <name>A0A8C4LPI4_EQUAS</name>
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        <recommendedName>
            <fullName evidence="11">G protein-coupled receptor kinase</fullName>
            <ecNumber evidence="11">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">GRK3</name>
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    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
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                <person name="Wang"/>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Q."/>
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                <person name="Y."/>
                <person name="Ju"/>
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                <person name="Pan"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
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                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
                <person name="Q."/>
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                <person name="C."/>
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                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[beta-adrenergic receptor] + ATP = [beta-adrenergic receptor]-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19429"/>
            <dbReference type="Rhea" id="RHEA-COMP:11222"/>
            <dbReference type="Rhea" id="RHEA-COMP:11223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.15"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:19430"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Presynapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.</text>
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    <dbReference type="EC" id="2.7.11.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_044631602.1">
        <property type="nucleotide sequence ID" value="XM_044775667.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LPI4"/>
    <dbReference type="Ensembl" id="ENSEAST00005014968.2">
        <property type="protein sequence ID" value="ENSEASP00005013787.2"/>
        <property type="gene ID" value="ENSEASG00005008029.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106841709"/>
    <dbReference type="KEGG" id="eai:106841709"/>
    <dbReference type="CTD" id="157"/>
    <dbReference type="GeneTree" id="ENSGT00940000157699"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0047696">
        <property type="term" value="F:beta-adrenergic receptor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004703">
        <property type="term" value="F:G protein-coupled receptor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002029">
        <property type="term" value="P:desensitization of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01240">
        <property type="entry name" value="PH_GRK2_subgroup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08747">
        <property type="entry name" value="RGS_GRK2_GRK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05633">
        <property type="entry name" value="STKc_GRK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000118">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000084">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000068">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000239">
        <property type="entry name" value="GPCR_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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    <dbReference type="Pfam" id="PF00169">
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    <dbReference type="PRINTS" id="PR00717">
        <property type="entry name" value="GPCRKINASE"/>
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    <dbReference type="SMART" id="SM00233">
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    <dbReference type="SMART" id="SM00315">
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    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
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    <dbReference type="SMART" id="SM00220">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
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    <dbReference type="SUPFAM" id="SSF56112">
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    <dbReference type="SUPFAM" id="SSF48097">
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    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="54"/>
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        <location>
            <begin position="191"/>
            <end position="453"/>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8C4LR53_EQUAS</name>
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        <submittedName>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1 3">Belongs to the 14-3-3 family.</text>
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        <property type="entry name" value="14-3-3"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="X. and Zhong"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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            <begin position="149"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="11">Cell autonomous antagonist of the canonical Wnt signaling pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with DVL1, DVL2, DVL3 and PPP2R3A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the NKD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044617192.1">
        <property type="nucleotide sequence ID" value="XM_044761257.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M4T8"/>
    <dbReference type="Ensembl" id="ENSEAST00005023098.2">
        <property type="protein sequence ID" value="ENSEASP00005021274.2"/>
        <property type="gene ID" value="ENSEASG00005014569.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822753"/>
    <dbReference type="KEGG" id="eai:106822753"/>
    <dbReference type="CTD" id="85407"/>
    <dbReference type="GeneTree" id="ENSGT00440000033589"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0030165">
        <property type="term" value="F:PDZ domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0003401">
        <property type="term" value="P:axis elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060026">
        <property type="term" value="P:convergent extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043410">
        <property type="term" value="P:positive regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000096">
        <property type="term" value="P:positive regulation of Wnt signaling pathway, planar cell polarity pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000166">
        <property type="entry name" value="Naked cuticle homolog 1 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040140">
        <property type="entry name" value="Nkd-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22611">
        <property type="entry name" value="PROTEIN NAKED CUTICLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22611:SF2">
        <property type="entry name" value="PROTEIN NAKED CUTICLE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0519">Myristate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="91"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="192"/>
            <end position="222"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="400"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="448"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="100"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="452"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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    <evidence type="ECO:0000256" key="8">
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00065729"/>
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    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367060"/>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50222"/>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005021274.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="470" mass="52032" checksum="160E9FC5F46452AF" modified="2023-09-13" version="2">MGKLHSKPAAVCKRRESPEGDSFAVSAAWARKGIEEWIGRQRCPGGSSGPRQLRAAGTVGRGTRELVGEVFRETLSEEEEEDFRLEVALPPEKPGALASGDEKRMEKSSDSCPGSRKQLKFEELQCDVSVEEDSRQEWTFTLYDFDNNGKVTREDITSLLHTIYEVVDSSVNHSPTSSKTLRVKLSVAPDGSQSKKSILLHHPDLQSTRPRAETKPTEELRSWERKQRAPLRFQGDSRLEQPGCSHRCVDENIERRNHYLDLAGVENYTSQFGAGSPSVAQKSELPPRACNPARSRSHEPEAIHVPHRKPQGTDPASFHFLDTPFAKVSEVQQRLRGTQDGSKHFVRSPKAQGKSVGVGHVARGARSKPPLGPAIPVVSPSAPLATSPALLPALAPLGHRKPKHRAKESQQGCRGLQGPLAPGGAVVGRDHVRELPTVVVYESQAGQAVQRHEHHHHHEHHHHYHHFYQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M5T6</accession>
    <name>A0A8C4M5T6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Calbindin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CALB1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with RANBP9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the calbindin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014708892.1">
        <property type="nucleotide sequence ID" value="XM_014853406.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M5T6"/>
    <dbReference type="Ensembl" id="ENSEAST00005022170.2">
        <property type="protein sequence ID" value="ENSEASP00005020421.2"/>
        <property type="gene ID" value="ENSEASG00005014050.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106838976"/>
    <dbReference type="KEGG" id="eai:106838976"/>
    <dbReference type="CTD" id="793"/>
    <dbReference type="GeneTree" id="ENSGT00950000183108"/>
    <dbReference type="OrthoDB" id="428774at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098982">
        <property type="term" value="C:GABA-ergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098686">
        <property type="term" value="C:hippocampal mossy fiber to CA3 synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099567">
        <property type="term" value="F:calcium ion binding involved in regulation of postsynaptic cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099534">
        <property type="term" value="F:calcium ion binding involved in regulation of presynaptic cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005499">
        <property type="term" value="F:vitamin D binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007626">
        <property type="term" value="P:locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007616">
        <property type="term" value="P:long-term memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072205">
        <property type="term" value="P:metanephric collecting duct development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072286">
        <property type="term" value="P:metanephric connecting tubule development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072221">
        <property type="term" value="P:metanephric distal convoluted tubule development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035502">
        <property type="term" value="P:metanephric part of ureteric bud development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900271">
        <property type="term" value="P:regulation of long-term synaptic potentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010842">
        <property type="term" value="P:retina layer formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007614">
        <property type="term" value="P:short-term memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd16176">
        <property type="entry name" value="EFh_HEF_CB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000108">
        <property type="entry name" value="Calbindin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000147">
        <property type="entry name" value="Calbindin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000054">
        <property type="entry name" value="Calbindin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051001">
        <property type="entry name" value="Calbindin_Ca-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029634">
        <property type="entry name" value="Calbindin_six-EFh_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19972">
        <property type="entry name" value="CALBINDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19972:SF14">
        <property type="entry name" value="CALBINDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00036">
        <property type="entry name" value="EF-hand_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0848">Vitamin D</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="11"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="98"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="142"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="186"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020421.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="261" mass="29961" checksum="8303AEBEC1431E0B" modified="2023-09-13" version="2">MAESHLQSSLITASQFFEIWLHFDADGSGYLEGKELQNLIQELQQARKKAGLELSPEMKTFVDHYGQKDDGKIGIVELAHVLPTEENFLLLFRCQQLKSCEEFMKTWRKYDTDHSGFIETEELKNFLKDLLEKANKTVDDTKLAEYTDLMLKLFDSNNDGKLELTEMARLLPVQENFLLKFQGVKMCGKEFNKAFELYDQDGNGYIDENELDALLKDLCEKNKQDLDINNIPTYKKSIMALSDGGKLYRTDLALILCAGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M7A3</accession>
    <name>A0A8C4M7A3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Heat shock 70 kDa protein 12A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HSPA12A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Adapter protein for SORL1, but not SORT1. Delays SORL1 internalization and affects SORL1 subcellular localization.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with SORL1 (via cytosolic C-terminus); this interaction affects SORL1 internalization and subcellular localization.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044606109.1">
        <property type="nucleotide sequence ID" value="XM_044750174.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M7A3"/>
    <dbReference type="Ensembl" id="ENSEAST00005023058.2">
        <property type="protein sequence ID" value="ENSEASP00005021234.2"/>
        <property type="gene ID" value="ENSEASG00005014531.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106836416"/>
    <dbReference type="CTD" id="259217"/>
    <dbReference type="GeneTree" id="ENSGT00940000154551"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051170">
        <property type="term" value="P:import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001889">
        <property type="term" value="P:liver development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042116">
        <property type="term" value="P:macrophage activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd11735">
        <property type="entry name" value="ASKHA_NBD_HSP70_HSPA12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000061">
        <property type="entry name" value="Heat shock protein family A (Hsp70) member 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000011">
        <property type="entry name" value="Heat shock protein family A (Hsp70) member 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026685">
        <property type="entry name" value="HSPA12A_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187">
        <property type="entry name" value="ALPHA KINASE/ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14187:SF46">
        <property type="entry name" value="HEAT SHOCK 70 KDA PROTEIN 12A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="17"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064411"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00070031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005021234.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="676" mass="75032" checksum="5447E8A86C0C376E" modified="2023-09-13" version="2">MADKEAGGSDTGPRETAPTSAYSSPARSLGDTGITPLSPSHVVNDADSNVSEQQTFLVVVAIDFGTTSSGYAYSFTKEPECIHVMRRWEGGDPGVSNQKTPTTILLTPERKFHSFGYAARDFYHDLDPNEAKQWLYLEKFKMKLHTTGDLTMDTDLTAANGKNVKALEIFAYALQYFKEQALKELSDQAGSDFENSDVRWVITVPAIWKQPAKQFMRQAAYQAGLASPENSEQLIIALEPEAASIYCRKLRLHQMIELSSKAAVNGYSSSDTVGAGFAQAKEHVRRNRQSRTFLVENVIGEIWSELEEGDKYVVVDSGGGTVDLTVHQIRLPEGHLKELYKATGGPYGSLGVDYEFEKLLCKIFGEDFIEQFKIKRPAAWVDLMIAFESRKRAAAPDRTNPLNITLPFSFIDYYKKFRGHSVEHALRKSNVDFVKWSSQGMLRMSPDAMNALFKPTIDSIIEHLRDLFQKPEVSTVKFLFLVGGFAEAPLLQQAVQAAFGDKCRIIVPQDVGLTILKGAVLFGLDPTVIKVRRSPLTYGVGVLNRYVEGKHPPEKLLVKDGTRWCTDVFDKFISADQSVALGELIKRSYTPAKPSQLVIIINIYSSEHDDVSFITDPGVKKCGTLRLDLTGTSGTAVPPRREIQTLMQFGDTEIKATAIDIATSKSVKVGIDFLNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M9A0</accession>
    <name>A0A8C4M9A0_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Undifferentiated embryonic cell transcription factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">UTF1</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M9A0"/>
    <dbReference type="Ensembl" id="ENSEAST00005023941.2">
        <property type="protein sequence ID" value="ENSEASP00005022064.2"/>
        <property type="gene ID" value="ENSEASG00005015070.2"/>
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    <dbReference type="GeneTree" id="ENSGT00390000014419"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0071837">
        <property type="term" value="F:HMG box domain binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044822">
        <property type="entry name" value="Myb_DNA-bind_4"/>
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    <dbReference type="Pfam" id="PF13837">
        <property type="entry name" value="Myb_DNA-bind_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like DNA-binding" evidence="2">
        <location>
            <begin position="65"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005022064.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="341" mass="36720" checksum="DF4EDF665BF8CCC8" modified="2023-09-13" version="2">MLLRPRRPPPPAPPLPSPSPARRDSEPRPAGEAPGTPPGRPASPGALAAPASPGSPVSPGSAQRTPWSAHETELLLGTLLQPAVWRALLLDRRRALPIYRRVSAALARQQVRRTPAQCRRRYKFLKDKLRDAHGQPPGPFDAQIRQLMGLLGDDGRKRGRRRSPGHRRGRRPAPDHPLRVPDAPPLLAARDSDADPAWTLRFNPFPPKSAETPHTPGSPTVLSTLSPVPGRPEDHAPFRAPSSPTPTPDPAWEDTDSPPGRPDDHAPPQPAPPSLNAALLQTLGHLGDIVAILGPLRDQLLTLNQHVEQLRGSFDQTVSLAVGFILGSAAAERGILADPRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M9U6</accession>
    <name>A0A8C4M9U6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Nardilysin</fullName>
            <ecNumber evidence="17">3.4.24.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">N-arginine dibasic convertase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Nardilysin convertase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">NRDC</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Cleaves peptide substrates on the N-terminus of arginine residues in dibasic pairs. Is a critical activator of BACE1- and ADAM17-mediated pro-neuregulin ectodomain shedding, involved in the positive regulation of axonal maturation and myelination. Required for proper functioning of 2-oxoglutarate dehydrogenase (OGDH).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Hydrolysis of polypeptides, preferably at -Xaa-|-Arg-Lys-, and less commonly at -Arg-|-Arg-Xaa-, in which Xaa is not Arg or Lys.</text>
            <dbReference type="EC" id="3.4.24.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with BACE1 and NRG1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.61" evidence="17"/>
    <dbReference type="RefSeq" id="XP_014688569.1">
        <property type="nucleotide sequence ID" value="XM_014833083.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M9U6"/>
    <dbReference type="Ensembl" id="ENSEAST00005022592.2">
        <property type="protein sequence ID" value="ENSEASP00005020810.2"/>
        <property type="gene ID" value="ENSEASG00005011045.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106826012"/>
    <dbReference type="KEGG" id="eai:106826012"/>
    <dbReference type="CTD" id="4898"/>
    <dbReference type="GeneTree" id="ENSGT00940000155026"/>
    <dbReference type="OrthoDB" id="4953at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000005">
        <property type="entry name" value="nardilysin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000017">
        <property type="entry name" value="nardilysin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000019">
        <property type="entry name" value="nardilysin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.830.10:FF:000027">
        <property type="entry name" value="nardilysin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001431">
        <property type="entry name" value="Pept_M16_Zn_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050626">
        <property type="entry name" value="Peptidase_M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032632">
        <property type="entry name" value="Peptidase_M16_M"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690:SF18">
        <property type="entry name" value="INSULIN-DEGRADING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43690">
        <property type="entry name" value="NARDILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16187">
        <property type="entry name" value="Peptidase_M16_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00143">
        <property type="entry name" value="INSULINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="22">
        <location>
            <begin position="284"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="23">
        <location>
            <begin position="438"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 middle/third" evidence="24">
        <location>
            <begin position="629"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="23">
        <location>
            <begin position="915"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="56"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="133"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="140"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00055893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00064814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00066846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00074237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00082392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF16187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020810.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1227" mass="140232" checksum="7F09A33542F0CA1E" modified="2023-09-13" version="2">MLGRVRVAAVCAAGRKLCEAGRELGALWRIEARGPREDSAAARPFSNLIMPGRNKAKSTCSCPDLQPGGQDLGESGRVARLGADESEEEGRRVSINNAGDPEIVKSPSDPKQYRYIKLRNGLQALLISDLSNMEGKMGDATDEEEEEEDEEEEEEEDDDDDDEDSGAEIEDDDEEGFDDEDEFDDDDERDDDDLDTEDNELEELEERAEARKKTTEKQQSQSLFLLWSKLTDILWFKSTFTKISSTLLVETRNLYGVVGAESRSAPVQHLAGWQEEEQQGETDTVLSAAALCVGVGSFADPDDLPGLAHFLEHMVFMGSLKYPDENGFDAFLKKHGGSDNASTDCERTVFQFDVQRKYFKEALDRWAQFFIHPLMIRDAIDREVEAVDSEYQLARPSDANRKEMLFGSLARPGHPMGKFFWGNAETLKHEPKRNNTDTHARLREFWMRYYSAHYMTLVVQSKETLDTLEKWVTEIFSQIPNNGLPKPNFGHLTDPFDTPAFNKLYRVVPIRKIHALTITWALPPQQQHYRVKPLHYISWLVGHEGKGSILSYLRKKCWALALFGGNGETGFEQNSTYSVFSISITLTDEGYEHFYEVAHTVFQYLKMLQKLGPEKRIFEEIQKIEDNEFHYQEQTDPVEYVENMCENMQLYPLQDFLTGDQLLFEYKPEVIAEALNQLVPPKANLVLLSGANEGKCDLKEKWFGTQYCMEDVENSWAELWKTNFELNPDLHLPAENKYIATDFMLKAFDCPETEYPVKIVNTPQGCLWYKKDNKFKIPKAYIRFHLISPLIQKSAANVVLFDIFVNILTHNLAEPAYEADVAQLEYKLVAGEHGLIIRVKGFNHKLPLLFQLIIDYLAEFSSTPAVFTMITEQLKKTYFNILIKPETLAKDVRLLILEYARWSMIDKYRALMDGLSLESLLSFVKEFKSQLFVEGLVQGNVTSTESMDFLKYVVDKLNFMPLEQEMPVQFQVVELPSGHHLCKVRALNKGDANSEVTVYYQSGTRSLREYTLMELLVMHMEEPCFDFLRTKQTLGYHVYPTCRNTSGILGFSVTVGTQATKYNSEVVDKKIEEFLSNFEEKIENLTEDAFNTQVTALIKLKECEDTHLGEEVDRNWNEVVTQQYLFDRLAHEIEALKSFSKSDLVNWFKAHRGPGCKMLGVHVVGFGKYELEEDGNPSGEDSNSSCEVMQLTYLPTSPLLADCTVPITDIRAFTSTLNLLPYHKIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4ME42</accession>
    <name>A0A8C4ME42_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106833350</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014700028.1">
        <property type="nucleotide sequence ID" value="XM_014844542.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4ME42"/>
    <dbReference type="Ensembl" id="ENSEAST00005027706.2">
        <property type="protein sequence ID" value="ENSEASP00005025515.1"/>
        <property type="gene ID" value="ENSEASG00005030090.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106833350"/>
    <dbReference type="KEGG" id="eai:106833350"/>
    <dbReference type="GeneTree" id="ENSGT00940000162052"/>
    <dbReference type="OMA" id="FTYLWPP"/>
    <dbReference type="OrthoDB" id="6151005at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15226">
        <property type="entry name" value="7tmA_OR4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000012">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050427">
        <property type="entry name" value="Olfactory_Receptors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48002">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="312" mass="35293" checksum="D3B35A706C0746E8" modified="2022-01-19" version="1">MEEANQSVVSEFIFRGLCHSREFQTFLLLPFSILYLMTIVGNLLVVSLIIRDPHLHSPMYFLLANLSFVDFCLSSVTTPKLTTDFLKDNKTISFQGCMSQILCVHFFGGGEMVLLVTMAYDRYVAICKPLHYSSIMNRQKCILLVLISWIIGFVHAMSQLAMILELPFCGPRIVDSFFCDIPLVIKLACMDTHTLGILINADSGLLATSCFIILLISYTYILVTVCLHSKDGASKALSTCTSHITVVVLFFGPCIFIYLWPLSITWVDKFLAVFYTVITPLLNPAIYTLRNKEIKNAIKRLKNQHIDSRDKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4ME79</accession>
    <name>A0A8C4ME79_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Solute carrier family 25 member 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phosphate carrier protein, mitochondrial</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Phosphate transport protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC106833192</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Inorganic ion transporter that transports phosphate or copper ions across the mitochondrial inner membrane into the matrix compartment. Mediates proton-coupled symport of phosphate ions necessary for mitochondrial oxidative phosphorylation of ADP to ATP. Transports copper ions probably in the form of anionic copper(I) complexes to maintain mitochondrial matrix copper pool and to supply copper for cytochrome C oxidase complex assembly. May also play a role in regulation of the mitochondrial permeability transition pore (mPTP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>phosphate(in) + H(+)(in) = phosphate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29939"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:29941"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with PPIF; the interaction is impaired by CsA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4ME79"/>
    <dbReference type="Ensembl" id="ENSEAST00005023064.2">
        <property type="protein sequence ID" value="ENSEASP00005021240.2"/>
        <property type="gene ID" value="ENSEASG00005014552.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008708"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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    <proteinExistence type="inferred from homology"/>
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    <sequence length="332" mass="36453" checksum="D70D08C83E31795D" modified="2023-09-13" version="2">MCEAGGCGAPTAGRSGPRPGRPWAAADEPGYEYGSRRYLLLCGLGGMLSCGLTHTAIVPLDLVKCRMQVDPGKYSGILSGFGVTLRDDGLRGLARGWAPTFLGYSLQGLFKFGLYEVFKIHYAERLGPERAYAWRTGLYLAASASAEFFADVALAPMEAVKVRIQTQPGFARTLRAAAPRMYGEEGLRAFYKGVAPLWLRQIPYTMMKFACFERTVEALYTFVVPKPQSQCSKAEQLAVTFVAGYIAGVFCAVVSHPADSVVSVLNKEKGSTAFGVLRRLGFWGVWKGLFARIIMIGTLTALQWFIYDSVKVYFKLPRPPVPQPPESLKEQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MGD3</accession>
    <name>A0A8C4MGD3_EQUAS</name>
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        <submittedName>
            <fullName evidence="5">SKI like proto-onco</fullName>
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    <gene>
        <name evidence="5" type="primary">SKIL</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SKI family.</text>
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        <property type="component" value="Chromosome 5"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:blastocyst formation"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:extrinsic apoptotic signaling pathway via death domain receptors"/>
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        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070306">
        <property type="term" value="P:lens fiber cell differentiation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002260">
        <property type="term" value="P:lymphocyte homeostasis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030512">
        <property type="term" value="P:negative regulation of transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902043">
        <property type="term" value="P:positive regulation of extrinsic apoptotic signaling pathway via death domain receptors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902231">
        <property type="term" value="P:positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd21084">
        <property type="entry name" value="DHD_Sno"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Putative ski oncogene"/>
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    </dbReference>
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        <property type="entry name" value="SKI-like oncogene a"/>
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        <property type="entry name" value="SAND domain-like"/>
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    <dbReference type="InterPro" id="IPR010919">
        <property type="entry name" value="SAND-like_dom_sf"/>
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        <property type="entry name" value="SKI/SNO/DAC"/>
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    <dbReference type="InterPro" id="IPR037000">
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    <dbReference type="InterPro" id="IPR023216">
        <property type="entry name" value="Tscrpt_reg_SKI_SnoN"/>
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    <dbReference type="PANTHER" id="PTHR10005">
        <property type="entry name" value="SKI ONCOGENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10005:SF3">
        <property type="entry name" value="SKI-LIKE PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF08782">
        <property type="entry name" value="c-SKI_SMAD_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02437">
        <property type="entry name" value="Ski_Sno_DHD"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM01046">
        <property type="entry name" value="c-SKI_SMAD_bind"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63763">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="c-SKI SMAD4-binding" evidence="4">
        <location>
            <begin position="261"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="418"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="537"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="459"/>
            <end position="473"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009513"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SMART" id="SM01046"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025293.2"/>
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        <source>
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        </source>
    </evidence>
    <sequence length="683" mass="77212" checksum="176FAB641E13AA92" modified="2023-09-13" version="2">MENLQTNFPLVQGSNKKLNGMGDDGSPPVKKMMTDIHANGKMMVNKMPTVKKEHLDDYEAPMETDGEHVKRTCASVPEPLHLNPSLKHTLAQFHLSSQSSLGGPAAFSARYSQESMSPTVFLPLPSPQVLPGPLLIPSDSSTELTQTLLEGESISCFQVGGEKRLCLPQVLNSVLREFSLQQINTVCDELYIYCSRCTSDQLHILKVLGILPFNAPSCGLITLTDAQRLCNALLRPRTFPQNGSILPAKNSLAQLKETGSAFEVEHECLGKCQGLFAPQFYVQPDAPCIQCLECCGMFAPQTFVMHSHRSPDKRTCHWGFESAKWHCYLHVNQKYLGTPEEKKLKKILEEMKEKFSVRNGKRTQSKANAPSGMELQSWYPVIKQEGDHVSQTHSFLPSYYLYMCDKVVAPNVSLTSAVSQSKEVAKTEASRPVPRQSEKPESSGKRQKTVSYPDVSLEEQEKMDLKTSRELCGRLDPSISSNSTSKKKPESTTCNLVRDTTKAGIDHDALVSSPLLVKDVICDDDKGKIMEEVMRTYVKQQEKLNSILQKKQQLQMEVEMLNSSKAMKELTEEQQNLQKELESLQNEHTQRMEEFYVEQKDLEKKLEQVMKQKCTCDSNLEKDKEAEYAAQLSELRQRLDHAEADRQELQDELRQEREARQKLEMMIKELKLQILKSSKTAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MIK6</accession>
    <name>A0A8C4MIK6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">FAD-dependent oxidoreductase domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">FOXRED2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Probable flavoprotein which may function in endoplasmic reticulum associated degradation (ERAD). May bind non-native proteins in the endoplasmic reticulum and target them to the ubiquitination machinery for subsequent degradation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with SEL1L. May interact with OS9 and DNAJC10. Interacts with TXNDC16.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the FOXRED2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MIK6"/>
    <dbReference type="Ensembl" id="ENSEAST00005030187.2">
        <property type="protein sequence ID" value="ENSEASP00005027793.2"/>
        <property type="gene ID" value="ENSEASG00005018907.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00640000091519"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000143">
        <property type="entry name" value="FAD-dependent oxidoreductase domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050982">
        <property type="entry name" value="Auxin_biosynth/cation_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539:SF23">
        <property type="entry name" value="FAD-DEPENDENT OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43539">
        <property type="entry name" value="FLAVIN-BINDING MONOOXYGENASE-LIKE PROTEIN (AFU_ORTHOLOGUE AFUA_4G09220)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13738">
        <property type="entry name" value="Pyr_redox_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040416635" description="FAD-dependent oxidoreductase domain-containing protein 2" evidence="12">
        <location>
            <begin position="27"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00068354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005027793.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="677" mass="76097" checksum="E0C10C819CBF5D22" modified="2023-09-13" version="1">MGLSAAAPLWGPPGLLLTIALHPALSLRPAKASAPPHRDYCVLGAGPAGLQMAYFLQRAGRDYAVFERAPEPGSFFTRYPRHRKLISINKRYTGKVNAEFNLRHDWNSLLSHDPRLLFRHYSRAYFPDAGDMVRYLRDFADRLRLHVLYNTTIAHVTLDKDPRAWNGHYFILTDQKGQAYRCSVLLVATGLSVPNQVDFPGSEYVEGYESVSVDPMDFVGQNVLILGRGNSAFETAENIFDVTNFIHMLSRSRVRLSWATHYVGDLRAINNGLLDTYQLKSLDGLLESDLTNLALVKDREGRFHVTLKYLLEETNQTADAITLPQDDNDNFAMRDAYDRVIRCLGWNFDFSIFNKSLRLSSGGEFSKKYPLVKASYESKGSRGLFVLGTASHSVDYRKSAGGFIHGFRYTVRAVHRLLEHRHHGVPWPSTEHPITQLTSSIVRRVNEASGLYQMFTVLADVVLLKEGATAFEYLEEFPMQMLAQLETITGRSAKHGLFVINMEYGRNFSGPDKDVFFYDRSVGHIEDAWQSNFLHPVIYYYRHLPTEQEVRFRPADWPLPRPTAIHHIRGGFPDRLDGPSGAHSTSEALPGELLRHRFAKLLCRVLFPVRPNAPEAAALLPAGVPENAGACGQRAPSSAWSRERAPAGLCRCGQAPEGQHPGSLETSLTLREAIDRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MM83</accession>
    <name>A0A8C4MM83_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Glutamate receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="primary">GRIN2C</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="26">Receptor for glutamate that functions as a ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18 26">Postsynaptic cell membrane</location>
            <topology evidence="18 26">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2C/GRIN2C subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MM83"/>
    <dbReference type="Ensembl" id="ENSEAST00005030591.2">
        <property type="protein sequence ID" value="ENSEASP00005028165.2"/>
        <property type="gene ID" value="ENSEASG00005019087.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156964"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017146">
        <property type="term" value="C:NMDA selective glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004972">
        <property type="term" value="F:NMDA glutamate receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904315">
        <property type="term" value="F:transmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097553">
        <property type="term" value="P:calcium ion transmembrane import into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033058">
        <property type="term" value="P:directional locomotion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060079">
        <property type="term" value="P:excitatory postsynaptic potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042177">
        <property type="term" value="P:negative regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050885">
        <property type="term" value="P:neuromuscular process controlling balance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903539">
        <property type="term" value="P:protein localization to postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048167">
        <property type="term" value="P:regulation of synaptic plasticity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06378">
        <property type="entry name" value="PBP1_iGluR_NMDA_NR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13718">
        <property type="entry name" value="PBP2_iGluR_NMDA_Nr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000026">
        <property type="entry name" value="Glutamate ionotropic receptor NMDA type subunit 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000020">
        <property type="entry name" value="Glutamate receptor ionotropic, NMDA 2B, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000007">
        <property type="entry name" value="Putative glutamate receptor ionotropic NMDA 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019594">
        <property type="entry name" value="Glu/Gly-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001508">
        <property type="entry name" value="Iono_Glu_rcpt_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015683">
        <property type="entry name" value="Ionotropic_Glu_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001320">
        <property type="entry name" value="Iontro_rcpt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018884">
        <property type="entry name" value="NMDAR2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18966">
        <property type="entry name" value="IONOTROPIC GLUTAMATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00060">
        <property type="entry name" value="Lig_chan"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10613">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10565">
        <property type="entry name" value="NMDAR2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00177">
        <property type="entry name" value="NMDARECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00918">
        <property type="entry name" value="Lig_chan-Glu_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00079">
        <property type="entry name" value="PBPe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 26" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="17 26" id="KW-0407">Ion channel</keyword>
    <keyword evidence="10 26" id="KW-0406">Ion transport</keyword>
    <keyword evidence="16 26" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15 26" id="KW-0628">Postsynaptic cell membrane</keyword>
    <keyword evidence="13 26" id="KW-0675">Receptor</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 26" id="KW-0732">Signal</keyword>
    <keyword evidence="9 26" id="KW-0770">Synapse</keyword>
    <keyword evidence="4 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 26" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040548011" description="Glutamate receptor" evidence="26">
        <location>
            <begin position="20"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="554"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="597"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="626"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="815"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor C-terminal" evidence="28">
        <location>
            <begin position="439"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="domain" description="Ionotropic glutamate receptor L-glutamate and glycine-binding" evidence="29">
        <location>
            <begin position="447"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="920"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="1016"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="27">
        <location>
            <begin position="928"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="27">
        <location>
            <begin position="968"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="27">
        <location>
            <begin position="1016"/>
            <end position="1038"/>
        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="510"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="515"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="686"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="687"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="728"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="24">
        <location>
            <position position="485"/>
        </location>
    </feature>
    <feature type="site" description="Crucial to convey clamshell closure to channel opening" evidence="24">
        <location>
            <position position="659"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with the cone snail toxin Con-ikot-ikot" evidence="24">
        <location>
            <position position="692"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="742"/>
            <end position="797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00060923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PIRSR" id="PIRSR601508-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU367118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="SMART" id="SM00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="SMART" id="SM00918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005028165.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1226" mass="133486" checksum="92556693183D5DC4" modified="2023-09-13" version="2">MGGALGPALLLTSLLGAWAGPGPGQREQTVTVAVVFGSSGLPQAQARTRLTPQSFLDLPLEIQPLTVGVNNTNPSSLLTQICGLLGAAHVHGIVFEDNVGTEAVAQILDFISSQTHVPILSISGGSAVVLTPKEPGSAFLQLGVSLEQQLQVLFKVLEEYDWSAFAVITSLHPGHALFLDGVRAVADASYVGWRLLDVLTLELGPGGPRARTQRLLRQVDAPVLVAYCSREEAEVLFAEAAQAGLVGPGHVWLVPNLALGSTDAPPASFPVGLISVVTESWRLSLRQKVRDGVAILALGAHGYRRQHGALPAPAGDCRSHPGPVSAAREAFYRHLLNVTWEGRDFSFSPGGYLAQPTMVVIALNRHRLWEMVGRWDHGILHMKYPVWPRYSASLQPVVDSRHLTVATLEERPFVIVESPDPGSGSCVPNTVPCRRQSNHTFSGDAAPYTKLCCKGFCIDILKKLARVVKFSYDLYLVTNGKHGKRVRGVWNGMIGEVYYKRADMAIGSLTINEERSEIVDFSVPFVETGISVMVARSNGTVSPSAFLEPYSPSVWVMMFVMCLTVVAITVFMFEYFSPVSYNQNLTSGKKSGGPSFTIGKSVWLLWALVFNNSVPIENPRGTTSKIMVLVWAFFAVIFLASYTANLAAFMIQEQYIDTVSGLSDKKFQRPQDQYPPFRFGTVPNGSTERNIRSNYRDMHTHMVKFNQRSVEDALTSLKMGKLDAFIYDAAVLNYMAGKDEGCKLVTIGSGKVFATTGYGIAMQKDSHWKRAIDLALLQFVGDGETQKLETVWLSGICQNEKNEVMSSKLDIDNMAGVFYMLLVAMGLALLVFAWEHLVYWKLRHSVPNTSHLDFLLAFSRGIYSCFSGVQSLASPARPPSPDLTADSAQASVLKMLQAARDMVATAGVSSSLDRATRTIESWGGGRRAPPPPACPGPRPPTPGPPPEPSPTGWGPPGGDRAAQGHGAPQPPGRPPTPGPPLPDVSRVPGRRAWEARWPVRARRGGWHISTSERRALPDRPLSPEHCHYSSFPRADRSGRPFLPLFPEPPEPEDLPLLGPEQLARREALLHAAWTQGPRPRHASLPSSVAEAFARPSSPPAGCVHRACGRLAQAQSMRLPTYREACQDGVWVGVPAWPHRQHACLHAHAHLPLCWRAVCPHLPPCASHSPWLAGAWGPPGHRSRTLGLSTGYRDSGELEEVSRVTCGTHAFQGPCTWRRISSLESEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MNG2</accession>
    <name>A0A8C4MNG2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Steroid hormone receptor ERR1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Estrogen-related receptor alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Nuclear receptor subfamily 3 group B member 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">ESRRA</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="17">Binds DNA as a monomer or a homodimer. Interacts (via the AF2 domain) with coactivator PPARGC1A (via the L3 motif); the interaction greatly enhances transcriptional activity of genes involved in energy metabolism. Interacts with PIAS4; the interaction enhances sumoylation. Interacts with MAPK15; promotes re-localization of ESRRA to the cytoplasm through a XPO1-dependent mechanism then inhibits ESRRA transcriptional activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 21">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 21">Belongs to the nuclear hormone receptor family. NR3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MNG2"/>
    <dbReference type="SMR" id="A0A8C4MNG2"/>
    <dbReference type="Ensembl" id="ENSEAST00005031878.2">
        <property type="protein sequence ID" value="ENSEASP00005029333.2"/>
        <property type="gene ID" value="ENSEASG00005019938.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160341"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
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        <property type="term" value="F:protein domain specific binding"/>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Steroid hormone receptor ERR1"/>
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        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
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        <property type="entry name" value="Retinoid X Receptor"/>
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        <property type="entry name" value="Est_rcpt/est-rel_rcp"/>
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        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
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    <sequence length="506" mass="54143" checksum="0217181736D9E261" modified="2023-09-13" version="2">MRTAGPAPAVSWRKQSRKRPRCPFVSYKPPAAPPSEGTRRGGAARPEEAAEEGPPAAPGSPRRSVPLGPHACPPALPEPQVTSAMSSQVVGIEPLYIKAEPASPDSPKGSSETETEPPVALASGPAPTRCLPGHKEEEDGEGAGPGEQGGGKLVLSSLPKRLCLVCGDVASGYHYGVASCEACKAFFKRTIQGSIEYSCPASNECEITKRRRKACQACRFTKCLRVGMLKEGVRLDRVRGGRQKYKRRPEVDPLPFPGPFPAGPLAVAGGPRKTAPVNALVSHLLVVEPEKLYAMPDPAGPDGQLPAVATLCDLFDREIVVTISWAKSIPGFSSLSLSDQMSVLQSVWMEVLVLGVAQRSLPLQDELAFAEDLVLDEEGARAAGLGELGAALLQLVRRLQALRLEREEYVLLKALALANSDSVHIEDAEAVEQLREALHEALLEYEAGRAGPGGGAERRRAGRLLLTLPLLRQTAGKVLAHFYGVKLEGKVPMHKLFLEMLEAMMD</sequence>
</entry>
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    <name>A0A8C4MQU5_EQUAS</name>
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        <submittedName>
            <fullName evidence="19">Transient receptor potential cation channel subfamily C member 7</fullName>
        </submittedName>
    </protein>
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        <name evidence="19" type="primary">TRPC7</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <reaction evidence="15">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
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            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
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            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="component" value="Chromosome 12"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:inositol 1,4,5 trisphosphate binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:store-operated calcium channel activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006828">
        <property type="term" value="P:manganese ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051480">
        <property type="term" value="P:regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007338">
        <property type="term" value="P:single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="short transient receptor potential channel 6 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000041">
        <property type="entry name" value="Transient receptor potential cation channel subfamily C member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013555">
        <property type="entry name" value="TRP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005463">
        <property type="entry name" value="TRPC7_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002153">
        <property type="entry name" value="TRPC_channel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00870">
        <property type="entry name" value="trp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10117:SF9">
        <property type="entry name" value="SHORT TRANSIENT RECEPTOR POTENTIAL CHANNEL 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10117">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
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                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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</entry>
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                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="GeneTree" id="ENSGT00940000162193"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="entry name" value="PH-EXOC1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Exocyst complex component 1 like"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.30.29.90">
        <property type="match status" value="1"/>
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        <property type="entry name" value="Sec3-PIP2_bind"/>
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    <dbReference type="Pfam" id="PF15277">
        <property type="entry name" value="Sec3-PIP2_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Exocyst complex component Sec3 PIP2-binding N-terminal" evidence="2">
        <location>
            <begin position="30"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00072567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005027700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="172" mass="20250" checksum="E5B4FDF1C96034A8" modified="2022-01-19" version="1">MSSLVKEDLEKKLFKPLSQNLYEFIEIEFSVQDRYYLCVSVTKNEEVQIIMVKHYRIGLDEKYEVTKKWSLNDLQMIDGKEADTDNPFFDLHFKKVYSLEAYSCASKYAFARTVNKLNHAYLKKDLQIVNFDSTYINDDSIWSSNNKDCLVLMRICFYAFNLVCLSLCPLPL</sequence>
</entry>
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    <name>A0A8C4MRT6_EQUAS</name>
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        <recommendedName>
            <fullName evidence="3">Adhesion G protein-coupled receptor B N-terminal domain-containing protein</fullName>
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    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
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            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR043838">
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        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
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        <property type="entry name" value="TSP1_rpt_sf"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
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    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040106658" description="Adhesion G protein-coupled receptor B N-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="28"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Adhesion G protein-coupled receptor B N-terminal" evidence="3">
        <location>
            <begin position="38"/>
            <end position="215"/>
        </location>
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        <location>
            <begin position="142"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030952.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="344" mass="36299" checksum="4B64FAF65C5FDB6B" modified="2023-09-13" version="2">MRGQAAAPGPLWILGLLLLLLGRRARAASGADVGPGSEPCATLVQGKFFGYFSAAAVFPANASRCSWTLRNPDPRRYTLYMKVAKAPVPCSGPGRVRTYQFDSFLESTRTYLGVESFDEVLRLCDPSAPLAFLQASKQFLQMRHQQPPRDGDPGPPAGPPGPGDDFSVEYLVVGNRNPSRAACQMLCRWLDACLAGSRSSHPCGIMQTPCACLGGDTGDPASSPLVPRGDVCLRDGMAGGPENCLTSLTQDRGGDGAPGGWKLWSLWGECTRDCGGGLQTRTRTCLPAKRERERRGDGTQREGSHGGQTGVGARLGALVPGFPTVQWGQVAVVLGSPAPTVAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MSY9</accession>
    <name>A0A8C4MSY9_EQUAS</name>
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        <submittedName>
            <fullName evidence="18">Potassium inwardly rectifying channel subfamily J member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">KCNJ4</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family.</text>
    </comment>
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    </dbReference>
    <dbReference type="RefSeq" id="XP_044635678.1">
        <property type="nucleotide sequence ID" value="XM_044779743.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_044779749.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_044779758.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070362974.1">
        <property type="nucleotide sequence ID" value="XM_070506873.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070362975.1">
        <property type="nucleotide sequence ID" value="XM_070506874.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070362976.1">
        <property type="nucleotide sequence ID" value="XM_070506875.1"/>
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        <property type="gene ID" value="ENSEASG00005018770.2"/>
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    <dbReference type="Ensembl" id="ENSEAST00005038511.1">
        <property type="protein sequence ID" value="ENSEASP00005050228.1"/>
        <property type="gene ID" value="ENSEASG00005018770.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005040289.1">
        <property type="protein sequence ID" value="ENSEASP00005043387.1"/>
        <property type="gene ID" value="ENSEASG00005018770.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005049016.1">
        <property type="protein sequence ID" value="ENSEASP00005060119.1"/>
        <property type="gene ID" value="ENSEASG00005018770.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005059160.1">
        <property type="protein sequence ID" value="ENSEASP00005062757.1"/>
        <property type="gene ID" value="ENSEASG00005018770.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005063961.1">
        <property type="protein sequence ID" value="ENSEASP00005045525.1"/>
        <property type="gene ID" value="ENSEASG00005018770.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005069797.1">
        <property type="protein sequence ID" value="ENSEASP00005054196.1"/>
        <property type="gene ID" value="ENSEASG00005018770.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005078374.1">
        <property type="protein sequence ID" value="ENSEASP00005053136.1"/>
        <property type="gene ID" value="ENSEASG00005018770.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005080760.1">
        <property type="protein sequence ID" value="ENSEASP00005057126.1"/>
        <property type="gene ID" value="ENSEASG00005018770.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005082017.1">
        <property type="protein sequence ID" value="ENSEASP00005051941.1"/>
        <property type="gene ID" value="ENSEASG00005018770.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837617"/>
    <dbReference type="KEGG" id="eai:106837617"/>
    <dbReference type="CTD" id="3761"/>
    <dbReference type="GeneTree" id="ENSGT01030000234586"/>
    <dbReference type="OMA" id="GGIIQMM"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005242">
        <property type="term" value="F:inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030165">
        <property type="term" value="F:PDZ domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="ATP-sensitive inward rectifier potassium channel 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000001">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003273">
        <property type="entry name" value="K_chnl_inward-rec_Kir2.3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF53">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005465">
        <property type="entry name" value="GIRK_kir"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01326">
        <property type="entry name" value="KIR23CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0630">Potassium</keyword>
    <keyword evidence="3 14" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0702">S-nitrosylation</keyword>
    <keyword evidence="4 14 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 14" id="KW-0813">Transport</keyword>
    <keyword evidence="6 14" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="57"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="16">
        <location>
            <begin position="22"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="17">
        <location>
            <begin position="184"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="site" description="Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium" evidence="13">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR005465-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005027566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="444" mass="49503" checksum="EBCDAD49C7802323" modified="2022-01-19" version="1">MHGHSRNGQAHVPRRKRRNRFVKKNGQCNVYFANLSNKSQRYMADIFTTCVDTRWRYMLMIFSAAFLVSWLFFGLLFWCIAFFHGDLEASPAVAAAGAPVGSGGAAPVAPKPCIMHVNGFLGAFLFSVETQTTIGYGFRCVTEECPLAVIAVVVQSIVGCVIDSFMIGTIMAKMARPKKRAQTLLFSHHAVISVRDGKLCLMWRVGNLRKSHIVEAHVRAQLIKPYMTQEGEYLPLDQRDLNVGYDIGLDRIFLVSPIIIVHEIDEDSPLYGMGKEELESEDFEIVVILEGMVEATAMTTQARSSYLASEILWGHRFEPVVFEEKSHYKVDYSRFHKTYEVAGTPCCSARELQESKITVLPAPPPPPSAFCYENELALMSQEEEEMEEEAAAAAAVAAGLGLEAGSKEEAGIIRMLEFGSHLDLERMQATLPLDNISYRRESAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MUY4</accession>
    <name>A0A8C4MUY4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 242</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TMEM242</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Scaffold protein that participates in the c-ring assembly of mitochondrial ATP synthase (F(1)F(0) ATP synthase or complex V) by facilitating the membrane insertion and oligomer formation of the subunit c/ATP5MC3. Participates in the incorporation of the c-ring into vestigial complexes. Additionally influences the incorporation of subunits MT-ATP6, MT-ATP8, ATP5MJ, and ATP5MK in the ATP synthase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM242 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MUY4"/>
    <dbReference type="Ensembl" id="ENSEAST00005030160.2">
        <property type="protein sequence ID" value="ENSEASP00005027770.2"/>
        <property type="gene ID" value="ENSEASG00005018892.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008642"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009792">
        <property type="entry name" value="TMEM242"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13141">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13141:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07096">
        <property type="entry name" value="DUF1358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005027770.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="127" mass="12947" checksum="D2F04C2EED0F5B5A" modified="2023-09-13" version="2">MEAAGAGSGEPAPRLEAPGSAEDRLFLVKGGIFLGTVAAAGMLAGFVTTLSLAKKKSPEWFNKGSVATAALPESGSSLALRALGWGSLYAWCGVGVISFAVWKALGVHSVSNCSPVMGVMFVLLYVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MWA9</accession>
    <name>A0A8C4MWA9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">Cyclin-dependent kinase-like 5</fullName>
            <ecNumber evidence="7">2.7.11.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="33" type="primary">CDKL5</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="26">Mediates phosphorylation of MECP2. May regulate ciliogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="27">Interacts with MECP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell junction</location>
            <location evidence="4">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4MWA9"/>
    <dbReference type="Ensembl" id="ENSEAST00005035892.2">
        <property type="protein sequence ID" value="ENSEASP00005032933.2"/>
        <property type="gene ID" value="ENSEASG00005022515.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157355"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097542">
        <property type="term" value="C:ciliary tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005922">
        <property type="term" value="C:connexin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032839">
        <property type="term" value="C:dendrite cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007154">
        <property type="term" value="P:cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045773">
        <property type="term" value="P:positive regulation of axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902017">
        <property type="term" value="P:regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050773">
        <property type="term" value="P:regulation of dendrite development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099175">
        <property type="term" value="P:regulation of postsynapse organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:001093">
        <property type="entry name" value="Cyclin-dependent kinase-like 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000127">
        <property type="entry name" value="Putative cyclin-dependent kinase-like 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.80">
        <property type="entry name" value="Gap junction channel protein cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000500">
        <property type="entry name" value="Connexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017990">
        <property type="entry name" value="Connexin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013092">
        <property type="entry name" value="Connexin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038359">
        <property type="entry name" value="Connexin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF111">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE-LIKE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00029">
        <property type="entry name" value="Connexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00206">
        <property type="entry name" value="CONNEXIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00037">
        <property type="entry name" value="CNX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00407">
        <property type="entry name" value="CONNEXINS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0965">Cell junction</keyword>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="23" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="21" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="17" id="KW-0303">Gap junction</keyword>
    <keyword evidence="15" id="KW-0418">Kinase</keyword>
    <keyword evidence="20 30" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="31" id="KW-0732">Signal</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="13 30" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19 30" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="31">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040507325" description="Cyclin-dependent kinase-like 5" evidence="31">
        <location>
            <begin position="21"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="32">
        <location>
            <begin position="103"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="388"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="470"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="733"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="407"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="470"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="495"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="522"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="561"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="582"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="637"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="767"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="816"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="857"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="29">
        <location>
            <begin position="905"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="951"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="29">
        <location>
            <begin position="968"/>
            <end position="979"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004610"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
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    <evidence type="ECO:0000256" key="6">
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            <dbReference type="ARBA" id="ARBA00006485"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00012425"/>
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    <evidence type="ECO:0000256" key="11">
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    <evidence type="ECO:0000256" key="12">
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    <evidence type="ECO:0000256" key="28">
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    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
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    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005032933.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1031" mass="116296" checksum="240B72875EAD7D57" modified="2023-09-13" version="2">MWLSVIFIFRILLLGTAVESAWDDEQFGFRCNTRQPGCENVCYDKSFPISHVCFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEEHKVAQTNGVNVEMHLQEIEIKKMKYGIEEHGKETHEIVAIKKFKDSEENEEVKETTLRELKMLRTLKQENIVELKEAFRRRGKLYLVFEYVEKNMLELLEEMPNGVPPEKVKSYIYQLIKAIHWCHKNDIVHRDIKPENLLISHNDVLKLCDFGFARNLSEGNNANYTEYVATRWYRSPELLLGAPYGKSVDMWSVGCILGELSDGQPLFPGESEIDQLFTIQKVLGPLPSEQMKLFYSNPRFHGLRFPAVNHPQSLERRYLGILNSVLLDLMKNLLKLDPADRYLTEQCLNHPTFQTQRLLDRSPSRSAKRKPYHVESSTLSNRNQASKSAALQSHHRSNSKDIQNLSVGLPRADEGLPANESFLNGNLAGATLSPMHTKTYQASSQPGSTSKDLTNNNMPHLLSPKEAKSKTEFDFNIDPKPSEGPGTKYLKSSSRSQQNRHSFMESSQSKAGTLQPSEKQSRHSYIDTIPQSSRSPSYRTKAKSHGALSDSKSVSNLSEARAQITESNTSRYFPSSCLDLNSPTSPTPTRHSDTRTLLSPSGRNNRSEGTLDSRRTTTRHSKTMEELKLPEHMDSSHSHSLSAPHESFSYGLGYTSPFSSQQRPHRHSMYVTRDKVRAKGLDGSLSVGQGMAARANSLQLLSPQPGEQLPPEMTVARSSVKESSREGPSSFHTRQKSEGGSYHDPHSDDGTAPKENRHLYNDPVPRRVGSFYRVPSPRPDNSFHENNVSTRVSSLPSESGSGTNHSKRQPAFDPWKSPENISHSEQLKEKEKQGFFRSMKKKKKKSQTVPNSDGPDLLTLQKAIHSASTPSSRPKEWRPEKISDLQTQSQPLKSLRKLLHLSSTSNHPASSDPRFQPLAAQQTKNSFSEIRIHPLSQASGGSSNIRQEPTPKGRPALQLPGLAVPRDDIRVPNFPQAAHDANYRSKRRVSSKKGSSSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N3J9</accession>
    <name>A0A8C4N3J9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
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                <person name="G."/>
                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
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                <person name="Yue"/>
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                <consortium name="Ensembl"/>
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                <consortium name="Ensembl"/>
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        <property type="entry name" value="KRAB"/>
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        <location>
            <begin position="40"/>
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        <name evidence="31" type="primary">CREBBP</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="Ji"/>
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                <person name="Jin"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="Amills"/>
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                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <text>(S)-lactoyl-CoA + L-lysyl-[protein] = N(6)-[(S)-lactoyl]-L-lysyl-[protein] + CoA + H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:231527"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:chromatin DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043993">
        <property type="term" value="F:histone H3K18 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044017">
        <property type="term" value="F:histone H3K27 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043426">
        <property type="term" value="F:MRF binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120300">
        <property type="term" value="F:peptide lactyltransferase (CoA-dependent) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:transcription coactivator activity"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0001223">
        <property type="term" value="F:transcription coactivator binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0048511">
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd05495">
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        <property type="match status" value="1"/>
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    </dbReference>
    <dbReference type="CDD" id="cd15557">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="RING_CBP-p300"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd02337">
        <property type="entry name" value="ZZ_CBP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Coactivator CBP, KIX domain"/>
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    <dbReference type="Gene3D" id="1.20.1020.10">
        <property type="entry name" value="TAZ domain"/>
        <property type="match status" value="2"/>
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        </source>
    </evidence>
    <sequence length="2452" mass="266194" checksum="F600A7A7CCAE54DB" modified="2023-09-13" version="2">MAENLLDGPPNPKRAKLSSPGFSANDSTDFGSLFDLENDLPDELIPNGGELSLLNSGNLVPDAASKHKQLSELLRGGSGSTINPGIGNVSASSPVQQGLGGQAQGQPNSANLGSLGAMGKSPLNQGDSSAPSLPKQAASTSGPTPPASQALNPQAQKQVGLVTSSPATSQTGPGICMNANFNQTHPGLLNSNSGHSLMNQAQQGQAQVMNGSLGAAGRGRGAGMPYPAPAMQGATSSVLAETLTQVSPQMASHAGLNTAQAGGMTKMGMTGNTSPFGQPFSQTGGQQMGAPGVNPQLPSKQSMVNSLPPFSTDIKNASVTNVPNMSQMQTAVGIVPTQAIATGPTADPEKRKLIQQQLVLLLHAHKCQRREQANGEVRACSLPHCRTMKNVLNHMTHCQAGKACQVAHCASSRQIISHWKNCTRHDCPVCLPLKNASDKRNQQTILGSPASGIQNTIGSVGTGQQNATSLSNPNPIDPSSMQRAYAALGLPYLNQPQTQLQPQVPGQQPAQPQTHQQMRTLNPLGNNPMNIPAGGITTDQQPPNLISESALPTSLGATNPLMNDGSNSGNIGTLSTIPTAAPPSSTGVRKGWHEHVTQDLRSHLVHKLVQAIFPTPDPAALKDRRMENLVAYAKKVEGDMYESANSRDEYYHLLAEKIYKIQKELEEKRRSRLHKQGILGNQPALPAPGAQPPGIPQAQPVRPPNGPMPLPVNRMPVSQGMNSFNPMPLGNVQLPQAPMGPRAASPMNHTVPMNSMGSVPGMAISPSRMPQPPNMMGGHANNMMAQAPAQNQFLPQNQFPSPSGALSVNSVGMGQPAAQTGVSQGQVPGAALPNPLNMLGPQGSQLPCPPVTQSPLHQTPPPASTAAGMPSLQHPTAPGMTPPQPAAPTQPSTPVSSSGQTPTPTPGSVPSASQTQSTPTVQAAAQAQVTPQPQTPVQPPSVATPQSSQQQPTPVHAQPPGTPLSQPAASIDNRVPTPSSVASAETNSQQPGPDVPMLEMKAEVKTEDTEPDASESKGEPGSAMMEEDLQGSSQVKEETDTAEQKSEPMEVDEKKPEVKVEAKEEEESSANGAVSQSTSPSQPRRKIFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDIVKNPMDLSTIKRKLDTGQYQEPWQYVDDVWLMFNNAWLYNRKTSRVYKFCSKLAEVFEQEIDPVMQSLGYCCGRKYEFSPQTLCCYGKQLCTIPRDAAYYSYQNSSPKYGLLADRYHFCEKCFTEIQGENVTLGDDPSQPQTTISKDQFEKKKNDTLDPEPFVDCKECGRKMHQICVLHYDIIWPSGFVCDNCLKKTGRTRKENKFSAKRLQTTRLGNHLEDRVNKFLRRQNHPEAGEVFVRVVASSDKTVEVKPGMKSRFVDSGEMSESFPYRTKALFAFEEIDGVDVCFFGMHVQEYGSDCPPPNTRRVYISYLDSIHFFRPRCLRTAVYHEILIGYLEYVKKLGYVTGHIWACPPSEGDDYIFHCHPPDQKIPKPKRLQEWYKKMLDKAFAERIIHDYKDIFKQATEDRLTSAKELPYFEGDFWPNVLEESIKELEQEEEERKKEESTAASETTEGSQGDSKNAKKKNNKKTNKNKSSISRANKKKPSMPNVSNDLSQKLYATMEKHKEVFFVIHLHAGPVINTLPPIVDPDPLLSCDLMDGRDAFLTLARDKHWEFSSLRRSKWSTLCMLVELHTQGQDRFVYTCNECKHHVETRWHCTVCEDYDLCINCYNTKSHTHKMVKWGLGLDDEGSSQGEPQSKSPQESRRLSIQRCIQSLVHACQCRNANCSLPSCQKMKRVVQHTKGCKRKTNGGCPVCKQLIALCCYHAKHCQENKCPVPFCLNIKHKLRQQQIQHRLQQAQLMRRRMATMNTRNVPQQSLPSPTSAPPGTPTQQPSTPQTPQPPAQPQPSPVSMSPASFPSVARTQPPTTVSTGKPTNQVPAPPPPAQPPPAAVEAARQIEREAQQQQHLYRVNINNGMPPGRTGMVTPVSQMAPVGLNVPRPNQVSGPVMPNLPPGQWQQAPIPQQQPMPGMPRPVIPMQAQPAVAGPRMSNVPPPRSISPGALQDLLRTLKSPSSPQQQQQVLNILKSNPQLMAAFIKQRTAKYVASQPGLQPQPGLQAQPGLQPQPGLHQQPGLQNLNAMQASGPRPGVPPQQQAMGGLNPQGQALNIMNPGHNPSMASMNPQYREMLRRQLLQQQQQQQQQQQQQQQQGSAGMAGGIAGHGQFQQPQGPGGYPPAMQQQRMQQHLPIQGSSMGQMAAQMGQLGQIGQPGLGADSTPNIQQALQQRILQQQQMKQQIGSPGQPNPMSPQQHMLSGQPQASHLPGQQMATSLSSQVRSPAPVQSPRPQSQPPHSSPSPRIQPQPSPHHVSPQTGSPHPGLAVTMASSIDQGHLGNPEQSAMLPQLNTPNRNALSSELSLVGDTTGDTLEKFVEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PGE9</accession>
    <name>A0A8C4PGE9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ATP-binding cassette sub-family F member 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ABCF2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PGE9"/>
    <dbReference type="Ensembl" id="ENSEAST00005003366.2">
        <property type="protein sequence ID" value="ENSEASP00005003079.2"/>
        <property type="gene ID" value="ENSEASG00005002063.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00630000089910"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000467">
        <property type="entry name" value="ATP-binding cassette sub-family F member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000104">
        <property type="entry name" value="ATP-binding cassette sub-family F member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF15">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="88"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="398"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="589"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="164"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="592"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="609"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003079.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="638" mass="72553" checksum="0E3266E9776BEACB" modified="2023-09-13" version="2">MPSDLAKKKAAKKKEAAKARQRPRKGHEENGDAVTEPQVAEEKNEEANGRETTEVDLLTKELEDFEMKKAAARAVTGVLASHPNSTDAHIINLSLTFHGQELLSDTKLELNSGRRYGLIGLNGIGKSMLLSAIGKREVPIPEHIDIYHLTREMPPSDKTPLQCVMEVDTERAMLEREAERLAHEDAECEKLLELYERLEELDADKAEMRASRILHGLGFTPAMQRKKLKDFSGGWRMRVALARALFIRPFMLLLDEPTNHLDLDACVWLEEELKTFKRILVLVSHSQDFLNGVCTNIIHMHNKKLKYYTGNYDQYVKTRLELEENQMKRFHWEQDQIAHMKNYIARFGHGSAKLARQAQSKEKTLQKMMASGLTERVVSDKTLSFYFPPCGKIPPPVIMVQNVSFKYTKDGPCIYNNLEFGIDLDTRVALVGPNGAGKSTLLKLLTGELLPTDGMIRKHSHVKIGRYHQHLQEQLDLDLSPLEYMMKCYPEIKEKEEMRKIIGRYGLTGKQQVSPIRNLSDGQKCRVCLAWLAWQNPHMLFLDEPTNHLDIETIDALADAINEFEGGMMLVSHDFRLIQQDLFSRLHKKSGSVRSRQSPSGLETSWPIRSTSSPSWWARSPSSPRGLTTCEPSPRPGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PGP5</accession>
    <name>A0A8C4PGP5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Opsin-3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Encephalopsin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Panopsin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">OPN3</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="19">Interacts with MC1R; the interaction results in a decrease in MC1R-mediated cAMP signaling and ultimately a decrease in melanin production in melanocytes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="nucleotide sequence ID" value="XM_070501624.1"/>
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        <property type="gene ID" value="ENSEASG00005003173.2"/>
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    <dbReference type="GeneTree" id="ENSGT01150000286935"/>
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        <property type="component" value="Chromosome 30"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005502">
        <property type="term" value="F:11-cis retinal binding"/>
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        <property type="term" value="F:all-trans retinal binding"/>
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        <property type="term" value="F:photoreceptor activity"/>
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        <property type="term" value="P:cellular response to UV-A"/>
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        <property type="term" value="P:keratinocyte differentiation"/>
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        <property type="term" value="P:negative regulation of apoptotic process"/>
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        <property type="term" value="P:negative regulation of melanin biosynthetic process"/>
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        <property type="term" value="P:positive regulation of D-glucose import"/>
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        <property type="entry name" value="7tmA_Encephalopsin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Opsin 3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="GPCR_opsins"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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    <dbReference type="InterPro" id="IPR027430">
        <property type="entry name" value="Retinal_BS"/>
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    <dbReference type="PANTHER" id="PTHR24240">
        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="7tm_1"/>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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        <property type="entry name" value="OPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0157">Chromophore</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11 23" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="18" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="15 23" id="KW-0675">Receptor</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="6" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="17 23" id="KW-0807">Transducer</keyword>
    <keyword evidence="7 23 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 25" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="78"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="157"/>
            <end position="176"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="202"/>
            <end position="223"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="257"/>
            <end position="280"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="292"/>
            <end position="311"/>
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    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="26">
        <location>
            <begin position="58"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1"/>
            <end position="25"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="24">
        <location>
            <begin position="1"/>
            <end position="18"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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            <dbReference type="ARBA" id="ARBA00022925"/>
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            <dbReference type="ARBA" id="ARBA00023040"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00023139"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00079531"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00083377"/>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="402" mass="44955" checksum="095CE513AE1C6EF2" modified="2023-09-13" version="2">MYAGNRSGGQGSWEGGAAAGAEDPGPAGPLSPAPLFSPGTYERLALLLGCLGLLGVGNNLLVLVLYSKFPRLRTPTHLLLVNISLSDLLVALFGVTFTFVSCLRNGWVWDAVGCAWDGFSSSLCGIVSITTLTVLAYERYIRVVHARVINFSWAWRALTYIWLYSLAWSGAPLLGWNRYILDIHGLGCAVDWKSKDANDSTFVLFLFLGCLVVPMGVIAHCYGHILYSIRMLRCVEDLQTIQVMKILRYEKKLAKMCFFMIFTFLIFWMPYIVICFLVANGYGHLVTPTVSIVSYLFAKSSTIYNPIIYIFTIRKFRRSLSQLLCFRLLRCQRPAKDQPPVGSEMQIRPIVMSQKDGDRPKKKVTFNSSSIIFIITSDESLSVHDSDKINGSKVEVIQVRPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PGW6</accession>
    <name>A0A8C4PGW6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Kinesin-like protein KIF21B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">KIF21B</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Plus-end directed microtubule-dependent motor protein which displays processive activity. Is involved in regulation of microtubule dynamics, synapse function and neuronal morphology, including dendritic tree branching and spine formation. Plays a role in lerning and memory. Involved in delivery of gamma-aminobutyric acid (GABA(A)) receptor to cell surface.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with TRIM3; the interaction positively affects motility of KIF21B. Interacts with GABARAP and GABA(A) receptor subunits: GABRG2, GABRA1 and GABRA2. May interact with GABA(A) receptor subunits: GABRB2 and GABRB3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Growth cone</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005004468.2">
        <property type="protein sequence ID" value="ENSEASP00005004078.2"/>
        <property type="gene ID" value="ENSEASG00005003075.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159650"/>
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        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005875">
        <property type="term" value="C:microtubule associated complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01372">
        <property type="entry name" value="KISc_KIF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22262">
        <property type="entry name" value="Rcc_KIF21B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000131">
        <property type="entry name" value="Kinesin family member 21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000158">
        <property type="entry name" value="Kinesin family member 21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000490">
        <property type="entry name" value="Kinesin family member 21B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000337">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000208">
        <property type="entry name" value="kinesin-like protein KIF21B isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056533">
        <property type="entry name" value="KIF21A/B_hel_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056532">
        <property type="entry name" value="KIF21A/B_hel_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027640">
        <property type="entry name" value="Kinesin-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969">
        <property type="entry name" value="CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47969:SF32">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF21B ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23203">
        <property type="entry name" value="KIF21A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23204">
        <property type="entry name" value="KIF21A_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25764">
        <property type="entry name" value="KIF21A_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 22" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="13 23" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="17" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="14 22" id="KW-0505">Motor protein</keyword>
    <keyword evidence="11 22" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 21" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Kinesin motor" evidence="25">
        <location>
            <begin position="80"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="21">
        <location>
            <begin position="1377"/>
            <end position="1416"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="21">
        <location>
            <begin position="1573"/>
            <end position="1612"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="21">
        <location>
            <begin position="1614"/>
            <end position="1655"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="21">
        <location>
            <begin position="1656"/>
            <end position="1686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="582"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="624"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="904"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="958"/>
            <end position="978"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="1271"/>
            <end position="1324"/>
        </location>
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    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="450"/>
            <end position="477"/>
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    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="510"/>
            <end position="577"/>
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    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="710"/>
            <end position="892"/>
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    </feature>
    <feature type="coiled-coil region" evidence="23">
        <location>
            <begin position="1007"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="24">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="24">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="24">
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            <begin position="582"/>
            <end position="605"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="24">
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            <begin position="650"/>
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            <begin position="920"/>
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            <begin position="1271"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="binding site" evidence="22">
        <location>
            <begin position="159"/>
            <end position="166"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
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            <dbReference type="ARBA" id="ARBA00004489"/>
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    <evidence type="ECO:0000256" key="4">
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    <evidence type="ECO:0000256" key="6">
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    <evidence type="ECO:0000256" key="11">
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    <evidence type="ECO:0000256" key="12">
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    <evidence type="ECO:0000256" key="14">
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    <evidence type="ECO:0000256" key="23">
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    <evidence type="ECO:0000256" key="24">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="25">
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    <evidence type="ECO:0000313" key="26">
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    <evidence type="ECO:0000313" key="27">
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PGW7</accession>
    <name>A0A8C4PGW7_EQUAS</name>
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            <fullName evidence="13">Amino acid transporter heavy chain SLC3A1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Neutral and basic amino acid transport protein</fullName>
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        <alternativeName>
            <fullName evidence="15">Solute carrier family 3 member 1</fullName>
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        <alternativeName>
            <fullName evidence="14">b(0,+)-type amino acid transporter-related heavy chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SLC3A1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Disulfide-linked heterodimer composed of the catalytic light subunit SLC7A9 and the heavy subunit SLC3A1. The heterodimer is the minimal functional unit. Assembles in non-covalently linked heterotetramers (dimers of heterodimers) and higher order oligomers; the oligomerization is mediated by SLC3A1 likely to prevent degradation in the endoplasmic reticulum and facilitate heteromer trafficking to the plasma membrane. Disulfide-linked heterodimer composed of the catalytic light subunit SLC7A13 and the heavy subunit SLC3A1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014688954.2">
        <property type="nucleotide sequence ID" value="XM_014833468.3"/>
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        <property type="nucleotide sequence ID" value="XM_044772337.2"/>
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        <property type="nucleotide sequence ID" value="XM_044772338.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_044772339.2"/>
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        <property type="term" value="C:brush border membrane"/>
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        <property type="entry name" value="Maltase A3, isoform A"/>
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        <property type="entry name" value="Solute carrier family 3 (amino acid transporter heavy chain), member 1"/>
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        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Golgi alpha-mannosidase II"/>
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        <property type="entry name" value="Oligo-1,6-glucosidase, Domain 2"/>
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        <property type="entry name" value="GH"/>
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    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
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    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
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    <dbReference type="InterPro" id="IPR045857">
        <property type="entry name" value="O16G_dom_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357">
        <property type="entry name" value="ALPHA-AMYLASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10357:SF179">
        <property type="entry name" value="NEUTRAL AND BASIC AMINO ACID TRANSPORT PROTEIN RBAT"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Alpha-amylase"/>
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        <property type="entry name" value="Aamy"/>
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    <keyword evidence="7" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 18" id="KW-0472">Membrane</keyword>
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    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="88"/>
            <end position="110"/>
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            <begin position="125"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="685" mass="78895" checksum="052F0A35AF5AC626" modified="2023-09-13" version="2">MAEDKSKKDSIGMSIKGGQTNNGFVQNEDILERDPDPDSPADSPQHTALHILGPTEPDLKDIRPYAGMPKEVLFQFSHQARYRVPREIIFWLTVASVLVLTGATIAIIAISPKCLDWWQAGPMYQIYPRSFKDSDKDGNGDLKGIQDKLDYIATLNIKTIWITSFYKSSLKDFRHGVEDFRDIDPIFGTMEDFENLLAAIHDKGLKLIIDFIPNHTSDKHPWFQLSRTRTGKYTDYYIWHDCTQENGITKPPNNWLSVYGNSSWHFDEVRNQCYFHQFMKEQPDLNFRSSDVQEEIKEIIQFWLAKGVDGFSFDAVKFLLEAKHLRDEPQVNKTQMPDTVTHYAELYHDFTTTQVGMHDIVRSFRQTMNQYSSEPGRYRFMGTEANGERIDRTMMYYGLPFIEEADFPFNDYLTRLETPSGSSVFEIITSWMENMPEGKWPNWMISGPDNARLMSRLGKEYVNVMNMLVFTLPGTPITYYGEEIGMGNILATNLNESYNVDTLFSKSPMQWDNSSNAGFSEGNHTWLPTSSDYHTVNVDVQKTQPTSALKLYQELSLLHANELLLSRGWFCHLRNDSHSVMYTRELDGIDTVFLVVLNFGESSLLNLKEMISDIPTRVRIRLSTNSAYKRSEVDTSAIVLDKGEGHILEYNTKNLLHHQTAFRDRCFISNRACYSSVFNILYSLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PH25</accession>
    <name>A0A8C4PH25_EQUAS</name>
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        <submittedName>
            <fullName evidence="2">NADH:ubiquinone oxidoreductase subunit V3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">NDUFV3</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <property type="component" value="Chromosome 18"/>
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        <property type="term" value="C:mitochondrial inner membrane"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042775">
        <property type="term" value="P:mitochondrial ATP synthesis coupled electron transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026193">
        <property type="entry name" value="NDUFV3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17117:SF3">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17117">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15880">
        <property type="entry name" value="NDUFV3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="397"/>
        </location>
    </feature>
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        <location>
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        </location>
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        <location>
            <begin position="543"/>
            <end position="568"/>
        </location>
    </feature>
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        <location>
            <begin position="215"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="251"/>
        </location>
    </feature>
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        <location>
            <begin position="289"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005008.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="610" mass="66607" checksum="458855D7C88F8E3F" modified="2023-09-13" version="2">MPGDTQPNDHFDVPAATLPVRRRVRIDQPACDWPRHQGAWSRSGRGAGAAWVRAQAQGSARPCPACDWCIWPRGRGRGRAVVVTRCRFPAPPLSLPVIGALVPGGRTSGGGARAVVTSVRARPPPRPARRPARPRVTVMAASLLLRQGRSRVLKTIFLEARVFRGLASTVSLSAESGKSEKGQPPNLKKQSPPKNVVEPKERGRLLATPAAAELSHSLSSPRSYPSVASEGGMVASPKPDDHRLFTDEGVPKRLSRKTLVEFPQKVPSPFRKQGSDPEALQQSRRGTDDSSSSSSSSSDSESDEEGDSSGADPQVTSKGKGRFPKPEGSHSFDSRAPKTAMSTKEKARSQQPPPDLPSPERPHQAKKKGTTAKLLEDRNGPKPAVPKSQVAGEFMKQNVKEKQLQKVFRSNEIEKERQKPFEVKKILSDHTKSGLSTQPNGGPAPTQLTEETTAGRQLRATAPEAREGGLEKQVPDPDGERAFPLFKKEDLGKQVIGTVKAKEEILEDQVPIKNLKPVPIHNKDVFDEKTAVLKLEEKGEIIEDSATQLGDQDDTQEPAQAAVPTEPFDNTTYKNLQHHDYNTFTFLDLNLELSKFRMPQPSSGRESPRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PHB4</accession>
    <name>A0A8C4PHB4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">4-hydroxyphenylpyruvate dioxygenase like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">HPDL</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 4HPPD family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014688791.1">
        <property type="nucleotide sequence ID" value="XM_014833305.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PHB4"/>
    <dbReference type="Ensembl" id="ENSEAST00005006063.2">
        <property type="protein sequence ID" value="ENSEASP00005005546.2"/>
        <property type="gene ID" value="ENSEASG00005004103.2"/>
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    <dbReference type="GeneID" id="106826134"/>
    <dbReference type="KEGG" id="eai:106826134"/>
    <dbReference type="CTD" id="84842"/>
    <dbReference type="GeneTree" id="ENSGT00530000063474"/>
    <dbReference type="OrthoDB" id="414569at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:4-hydroxymandelate synthase activity"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:4-hydroxyphenylpyruvate dioxygenase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0009072">
        <property type="term" value="P:aromatic amino acid metabolic process"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="CDD" id="cd07250">
        <property type="entry name" value="HPPD_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08342">
        <property type="entry name" value="HPPD_N_like"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005956">
        <property type="entry name" value="4OHPhenylPyrv_dOase"/>
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    <dbReference type="InterPro" id="IPR041735">
        <property type="entry name" value="4OHPhenylPyrv_dOase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041736">
        <property type="entry name" value="4OHPhenylPyrv_dOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11959:SF10">
        <property type="entry name" value="4-HYDROXYPHENYLPYRUVATE DIOXYGENASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="VOC" evidence="7">
        <location>
            <begin position="7"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="VOC" evidence="7">
        <location>
            <begin position="160"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005546.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="371" mass="38960" checksum="D6E502B0B71E3FD7" modified="2023-09-13" version="2">MAAPARSLCHIAFHVPAGQPLAQDLQRIFGFQPLAAREADGWLQLALRSGDAVFLVNEGGGRGEPLYGLDPRHAVPSATNLCFDVADVGAAARALASRGCSVPVAPVSVRDAQGTATYAVVSSPAGNLSLTLLERAGFRGPFLPGFGPVPSATGPGLVSHVDHLTLACTPGSSPTLMRWFHDCLGFRHLPLSPGEDPELGLEVEAGSGRGGLRLTALQTPPGSAVPTLLLAESLLGATDGQDQIEQFLAQHRGPGLQHVGLYTPNILKATERVAVAGGQLLTPPSAYYLQPGKERQILAAGHEPSLLARQGILLDGDKGKFLLQVFTKSLFAEDTFFLELIQRQGATGFGQGNIRALWQSVQEQAARGQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8C4PKH0_EQUAS</name>
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        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
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        <name evidence="16" type="primary">LOC106843459</name>
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    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 14">Cell membrane</location>
            <topology evidence="3 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PKH0"/>
    <dbReference type="Ensembl" id="ENSEAST00005013433.2">
        <property type="protein sequence ID" value="ENSEASP00005012368.2"/>
        <property type="gene ID" value="ENSEASG00005025959.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244828"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15227">
        <property type="entry name" value="7tmA_OR14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000037">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="57"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005012368.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="308" mass="34418" checksum="E3530C3A5F376F2A" modified="2023-09-13" version="2">MANFTTLKGFLLMGFSAKPEIEIVCASMFLVLYLVALTGNILIITATSIDDSLHSPLYFFLKHLSFLDLCYISVTVPRYICNSFMHNGNISLWECILQCFAFTLCGSSEMAMLTVMSYDRYVAICLPLRYEIIMNVSTCIHGILAVWISGVISGVMHTAATFSIHFCGANIIHQFFCDVPQLLKFSCSNEYVGELGVILFLSLVAFLCFICIGLSYMHIFSTVLQIPSAEGRAKAFSTCLPHLSVVMLFLSTGVFDFLKPHSDSPTALDFLLTVFYTVLPPALNPMIYSLRNKAMKTALRKVFKRRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PMJ3</accession>
    <name>A0A8C4PMJ3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Centrosomal protein of 97 kDa</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Leucine-rich repeat and IQ domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CEP97</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as a key negative regulator of ciliogenesis in collaboration with CCP110 by capping the mother centriole thereby preventing cilia formation. Required for recruitment of CCP110 to the centrosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PMJ3"/>
    <dbReference type="Ensembl" id="ENSEAST00005017576.2">
        <property type="protein sequence ID" value="ENSEASP00005016175.2"/>
        <property type="gene ID" value="ENSEASG00005011204.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106838877"/>
    <dbReference type="KEGG" id="eai:106838877"/>
    <dbReference type="CTD" id="79598"/>
    <dbReference type="GeneTree" id="ENSGT00910000144283"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902018">
        <property type="term" value="P:negative regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000165">
        <property type="entry name" value="Centrosomal protein of 97 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000199">
        <property type="entry name" value="centrosomal protein of 97 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF2">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 97 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="649"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="715"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="585"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="649"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="720"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="736"/>
            <end position="762"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00076677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005016175.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="860" mass="95861" checksum="B11E1D855D548E93" modified="2023-09-13" version="2">MAVARVDAALPPGDGSVVNWSGQGLQKLSPNLPCEADIHTLILDKNQIIKLENLEKCRRLIQLSVANNRLVRMMGVAKLTQLRVLNLPHNSIGYVEGLKELVHLEWLNLAGNNLKAMEQINSCAALQHLDLSDNNIPQIGDLSKLLSLKTLLLHGNIITSLRMAPAYLPRSLAILSLAENEVRDLNEISFLASLTELEQLSIMNNPCVMATPSIPGFDYRPYIVSWCLNLRVLDGYVISQKESLKAEWLYSQGKGRAYRPGQHIQLVQYLATVCPLTSTLGLQTAEDAKLEKILSKQRFHQRQLMNQSQNEELSPLAPVETRAPLVPEHSSPVQDCQIVQESEPVIQVNSWVGISSDDDQLYGVKNNFPASVHTARYSRNDLRLEDIQTDEDKLSCSLLSSESTFMPVASGLSPVSPTVELRLQGINLGLEDDGVADESVKGLGNQDFDKEEEKAFWAAKENSVQAMKSPISTEVNEKDGPLPCPEPTVTSAVLKDDTHSLTSSPESVGHNVFHTQPDGEEIMSQTASEKLPCRVLTQRCVALGQDKVALQKLNEAATKLQACWRGFYARNYNPQAKDVRYEIRLRRMQEHIVCLTDEIRRLRKERDEERIKKFVQEEAVRFLWNQVRSLQAWQQTVDQRLSSWHSDVPPLSSTLVPSKPPSFTQSQESSSDHNSDWFIAPDEAPQEKSLREFPDSGFHSSLTEQIHCLQDSLDFEKSSTEGSESSIMGNSVDTVKYGKESDVGDVSEEHGEWSKESSNDEQDKSLLEQYLTSVQQLEDADERTNFDEGTGDSKLYIACSPEKLDVLSDIASVDDTHGLSPALQAEISQTPENCKLNAEVQGQQSECESTFQVLHVGVIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PN56</accession>
    <name>A0A8C4PN56_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Argonaute RISC catalytic component 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">AGO3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the argonaute family. Ago subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PN56"/>
    <dbReference type="Ensembl" id="ENSEAST00005018986.2">
        <property type="protein sequence ID" value="ENSEASP00005017483.2"/>
        <property type="gene ID" value="ENSEASG00005012089.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155256"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016442">
        <property type="term" value="C:RISC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070578">
        <property type="term" value="C:RISC-loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090624">
        <property type="term" value="F:endoribonuclease activity, cleaving miRNA-paired mRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035198">
        <property type="term" value="F:miRNA binding"/>
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    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
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        <property type="term" value="P:miRNA-mediated gene silencing by inhibition of translation"/>
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        <property type="term" value="P:mRNA catabolic process"/>
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        <property type="term" value="P:positive regulation of gene expression"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:regulation of stem cell proliferation"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0070922">
        <property type="term" value="P:RISC complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="CDD" id="cd02846">
        <property type="entry name" value="PAZ_argonaute_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04657">
        <property type="entry name" value="Piwi_ago-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein argonaute-2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Protein argonaute-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014811">
        <property type="entry name" value="ArgoL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032472">
        <property type="entry name" value="ArgoL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032473">
        <property type="entry name" value="Argonaute_Mid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032474">
        <property type="entry name" value="Argonaute_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
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    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
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    <dbReference type="InterPro" id="IPR045246">
        <property type="entry name" value="Piwi_ago-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08699">
        <property type="entry name" value="ArgoL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16488">
        <property type="entry name" value="ArgoL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16487">
        <property type="entry name" value="ArgoMid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16486">
        <property type="entry name" value="ArgoN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01163">
        <property type="entry name" value="DUF1785"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="PAZ" evidence="7">
        <location>
            <begin position="230"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="8">
        <location>
            <begin position="518"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008201"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005017483.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="793" mass="89567" checksum="C3F83C905307FF6B" modified="2023-09-13" version="2">MEIGSAGPVGAQPLLMVPRRPGYGTMGKPIKLLANCFQVEIPKIDVYLYEVDIKPDKCPRRVNREVVDSMVQHFKVTIFGDRRPVYDGKRSLYTANPLPVATTGVDLDVTLPGEGGKDRPFKVSIKFVSRVSWHLLHEVLTGRTLPEPLELDKPISTNPVHAVDVVLRHLPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQAEGSHVSGQSNGRDPQALAKAVQIHQDTLRTMYFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PPN7</accession>
    <name>A0A8C4PPN7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein polybromo-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">BRG1-associated factor 180</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">PBRM1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Required for the stability of the SWI/SNF chromatin remodeling complex SWI/SNF-B (PBAF). Acts as a negative regulator of cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the SWI/SNF-B (PBAF) chromatin remodeling complex, at least composed of SMARCA4/BRG1, SMARCB1/BAF47/SNF5, ACTL6A/BAF53A or ACTL6B/BAF53B, SMARCE1/BAF57, SMARCD1/BAF60A, SMARCD2/BAF60B, perhaps SMARCD3/BAF60C, SMARCC1/BAF155, SMARCC2/BAF170, PBRM1/BAF180, ARID2/BAF200 and actin. Interacts with PHF10/BAF45A. Interacts with acetylated 'Lys-14' of histone H3 (H3K14ac), and may also interact with other acetylated or methylated Lys residues on histone H3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005023170.2">
        <property type="protein sequence ID" value="ENSEASP00005021344.2"/>
        <property type="gene ID" value="ENSEASG00005013418.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003017"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04717">
        <property type="entry name" value="BAH_polybromo"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05524">
        <property type="entry name" value="Bromo_polybromo_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05517">
        <property type="entry name" value="Bromo_polybromo_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05520">
        <property type="entry name" value="Bromo_polybromo_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05518">
        <property type="entry name" value="Bromo_polybromo_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05515">
        <property type="entry name" value="Bromo_polybromo_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05526">
        <property type="entry name" value="Bromo_polybromo_VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21984">
        <property type="entry name" value="HMG-box_PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000009">
        <property type="entry name" value="Protein polybromo-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000011">
        <property type="entry name" value="Protein polybromo-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000013">
        <property type="entry name" value="Protein polybromo-1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000006">
        <property type="entry name" value="protein polybromo-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000010">
        <property type="entry name" value="protein polybromo-1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000015">
        <property type="entry name" value="protein polybromo-1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000002">
        <property type="entry name" value="protein polybromo-1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.490:FF:000003">
        <property type="entry name" value="protein polybromo-1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001025">
        <property type="entry name" value="BAH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037968">
        <property type="entry name" value="PBRM1_BD5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037382">
        <property type="entry name" value="Rsc/polybromo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062:SF19">
        <property type="entry name" value="PROTEIN POLYBROMO-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062">
        <property type="entry name" value="SWI/SNF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01426">
        <property type="entry name" value="BAH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
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        <property type="entry name" value="BAH"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="BROMO"/>
        <property type="match status" value="6"/>
    </dbReference>
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        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
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        <property type="match status" value="6"/>
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        <property type="entry name" value="HMG-box"/>
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    <dbReference type="PROSITE" id="PS00633">
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    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
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            <begin position="64"/>
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            <begin position="1379"/>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PS85</accession>
    <name>A0A8C4PS85_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C-X-C motif chemokine 16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Transmembrane chemokine CXCL16</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">CXCL16</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intercrine alpha (chemokine CxC) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PS85"/>
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        <property type="protein sequence ID" value="ENSEASP00005026210.1"/>
        <property type="gene ID" value="ENSEASG00005017868.2"/>
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        <property type="component" value="Chromosome 13"/>
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    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005041">
        <property type="term" value="F:low-density lipoprotein particle receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005044">
        <property type="term" value="F:scavenger receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030307">
        <property type="term" value="P:positive regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034612">
        <property type="term" value="P:response to tumor necrosis factor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034341">
        <property type="term" value="P:response to type II interferon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010818">
        <property type="term" value="P:T cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026296">
        <property type="entry name" value="CXCL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048585">
        <property type="entry name" value="CXCL16_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14385:SF0">
        <property type="entry name" value="C-X-C MOTIF CHEMOKINE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14385">
        <property type="entry name" value="CXC CHEMOKINE LIGAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20902">
        <property type="entry name" value="CXCL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="5" id="KW-0202">Cytokine</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15" id="KW-0732">Signal</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034843736" description="C-X-C motif chemokine 16" evidence="15">
        <location>
            <begin position="26"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="domain" description="C-X-C motif chemokine 16" evidence="16">
        <location>
            <begin position="23"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="105"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20902"/>
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    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026210.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="247" mass="27133" checksum="B11D6BC474EE1FD1" modified="2022-01-19" version="1">MRRRRGPLPFELLLLLALLTLQGDGNEGSIAGSCRCNTRFPSDSPPAVHIMKHFRKQLRGYDRCGSYVRFQLHSRSVCGGSKDQWVHELISCFDRKECGRGNLGRVAHREHLPPPSTQAPEPTERASSDVVTPAQTYLPSTLQPIQQSTRPAATPSLDKNLIHANETTASTLGHSLVSAPEAGENQKQLKEHVGSAIGTSVTVAVPSLLAIVFFLTGVLLYVLCKKREKTLRPSPDLQAHYERVAPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PSW8</accession>
    <name>A0A8C4PSW8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Host cell factor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">C1 factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">HCFC1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044619047.1">
        <property type="nucleotide sequence ID" value="XM_044763112.2"/>
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    <dbReference type="Ensembl" id="ENSEAST00005030761.2">
        <property type="protein sequence ID" value="ENSEASP00005028328.2"/>
        <property type="gene ID" value="ENSEASG00005019244.2"/>
    </dbReference>
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    <dbReference type="KEGG" id="eai:106825608"/>
    <dbReference type="CTD" id="3054"/>
    <dbReference type="GeneTree" id="ENSGT00940000161383"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0071339">
        <property type="term" value="C:MLL1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044545">
        <property type="term" value="C:NSL complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031490">
        <property type="term" value="F:chromatin DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001835">
        <property type="term" value="P:blastocyst hatching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043254">
        <property type="term" value="P:regulation of protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019046">
        <property type="term" value="P:release from viral latency"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000443">
        <property type="entry name" value="host cell factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000259">
        <property type="entry name" value="Host cell factor 1 (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000008">
        <property type="entry name" value="host cell factor 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000015">
        <property type="entry name" value="host cell factor 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043536">
        <property type="entry name" value="HCF1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059124">
        <property type="entry name" value="Kelch_HCF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46003">
        <property type="entry name" value="HOST CELL FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46003:SF3">
        <property type="entry name" value="HOST CELL FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13854">
        <property type="entry name" value="Kelch_HCF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="3" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="19">
        <location>
            <begin position="1948"/>
            <end position="2064"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="407"/>
            <end position="435"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="18">
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            <begin position="1307"/>
            <end position="1377"/>
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    <feature type="region of interest" description="Disordered" evidence="18">
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            <begin position="1447"/>
            <end position="1482"/>
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    <feature type="region of interest" description="Disordered" evidence="18">
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            <begin position="1558"/>
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    <feature type="region of interest" description="Disordered" evidence="18">
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            <begin position="2052"/>
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            <begin position="413"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="1311"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="1341"/>
            <end position="1364"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1450"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
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        <source>
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    <evidence type="ECO:0000256" key="6">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00074287"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081526"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50853"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005028328.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="2093" mass="214439" checksum="EEFDCD74B2661C73" modified="2023-09-13" version="2">MASAVSPANSPAVLLQPRWKRVVGWSGPVPRPRHGHRAVAIKELIVVFGGGNEGIVDELHVYNTATNQWFIPAVRGDIPPGCAAYGFVCDGTRLLVFGGMVEYGKYSNDLYELQASRWEWKRLKAKTPKNGPPPCPRLGHSFSLVGNKCYLFGGLANDSEDPKNNIPRYLNDLYILELRPGSGVVAWDIPITYGVLPPPRESHTAVVYTEKDNKKSKLVIYGGMSGCRLGDLWTLDIETLTWNKPSLSGVAPLPRSLHSATTIGNKMYVFGGWVPLVMDDVKVATHEKEWKCTNTLACLNLDTMAWETILMDTLEDNIPRARAGHCAVAINTRLYIWSGRDGYRKAWNNQVCCKDLWYLETEKPPPPARVQLVRANTNSLEVSWGAVATADSYLLQLQKYDIPATAATATSPTPNPVPSVPTNPPKSPAPAAAAPAVQPLTQVGITLLPQAAAAPPTTTTIQVLPTVPGSSISVPTAARTQGVPAVLKVTGPQATTGTPLVTMRPASQAGKAPVTVTSLPAGVRMVVPTQSAQGTVIGSSPQMSGMAALAAAAAATQKIPPSSAPTVLSVPAGTTIVKTVAVTPGTTTLPATVKVASSPVMVSNPATRMLKTAAAQVGTSVSSAANTSTRPIITVHKSGTVTVAQQAQVVTTVVGGVTKTITLVKSPISVPGGSALISNLGKVMSVVQTKPVQTSAVTGQASTGPVTQIIQTKGPLPAGTILKLVTSADGKPTTIITTTQASGAGTKPTILGISSVSPSTTKPGTTTIIKTIPMSAIITQAGATGVTSSPGIKSPITIITTKVMTSGTGAPAKIITAVPKIATGHGQQGVTQVVLKGAPGQPGTILRTVPMGGVRLVTPVTVSAVKPAVTTLVVKGTTGVTTLGTVTGTVSTSLAGAGGHSTSASLATPITTLGTIATLSSQVINPTAITVSAAQTTLTAASGLTTPTITMQPVSQPTQVTLITAPSGVEAQPVHDLPVSILASPTTEQPTATVTIADSGQGDVQPGTVTLVCSNPPCETHETGTTNTATTTVVANLGGHSQPAQVQFVCDRQEAAASLVTSPVGQQNGSVVRVCSNPPCETHETGTTSTATTATSNMAGQHGCSNPPCETHETGTTSTATTAMSTIGTGQQRDARHAYVASTAPAVVRVSLASGASQGAQGSVKPSCQTCQTSATSTTMTVMATSAPLLGPSLVLEAGGHSTTFVQLAPVSSQVRPSGLGGKDSPIAGLGQLVSAGHQLEAHHTHTTNTPTTARSTMGAAELGEAQGTLIPVYESSSGAAVTVTALEALLCPSATVVQVCSNPPCETHETGTTNTATTSSAGSAQRVCSNPPCETHETGTTHTPTTATSSGAAGQPEGGQQPPAGHPCETHQTTSTGTTMSVSVGALLPATTPSPRTLESTLEVAAPPTVTPQAGVSLLAPFPTQRVCSNPPCETHETGTTHTATTVTSNMSSNQDPPPATSDQGEVESTQSDSVNLTSSSAITTTVSSTLTRAVTTVTQSTPVPGPSVPKISSMTETTPGALTTEVPIPATITVTIANTETSDMPFSAVDILQPPEELQASPGPRQQLPPRQLLQPASTPLMGESTEVLSASQTPELQAAVDLSSTGDSSSGQEPASSAVVATVVVQPPPPTQSEVDQLSLPQELMAEAQAGTTTLMVTGLTPEELAVTAAAEAAAQAAATEEAQALAIQAVLQAAQQAVMAGTGEPMDTSEAAAAVTQAELGHLSAEGQEGQATTIPIVLTQQELAALVQQQQQLQEAQAQQQHHHLPTEALAPADSLNDPTIESNCLNELAGAVPSTVALLPSTATESLAPSNTFVAPQPVVVASPAKLQAAATLTEVANGIESLGVKPDLPPPPSKAPVKKENQWFDVGVIKGTNVMVTHYFLPPDDAVPSDDDSGTVPDYNQLKKQELQPGTAYKFRVAGINACGRGPFSEISAFKTCLPGFPGAPCAIKISKSPDGAHLTWEPPSVTSGKIIEYSVYLAIQSSQAGGEPKSSTPAQLAFMRVYCGPSPSCLVQSSSLSNAHIDYTTKPAIIFRIAARNEKGYGPATQVRWLQETSKDSSGAKPASKRPMSSPEMKSAPKKSKADGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PTC2</accession>
    <name>A0A8C4PTC2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Neuroendocrine convertase 1</fullName>
            <ecNumber evidence="6">3.4.21.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Prohormone convertase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Proprotein convertase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">PCSK1</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of protein hormones, neuropeptides and renin from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.</text>
            <dbReference type="EC" id="3.4.21.93"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasmic vesicle</location>
            <location evidence="4">Secretory vesicle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase S8 family. Furin subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.93" evidence="6"/>
    <dbReference type="RefSeq" id="XP_014722994.1">
        <property type="nucleotide sequence ID" value="XM_014867508.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PTC2"/>
    <dbReference type="Ensembl" id="ENSEAST00005031005.2">
        <property type="protein sequence ID" value="ENSEASP00005028541.2"/>
        <property type="gene ID" value="ENSEASG00005019297.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106847921"/>
    <dbReference type="KEGG" id="eai:106847921"/>
    <dbReference type="CTD" id="5122"/>
    <dbReference type="GeneTree" id="ENSGT00940000157385"/>
    <dbReference type="OrthoDB" id="300641at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034774">
        <property type="term" value="C:secretory granule lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030133">
        <property type="term" value="C:transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030070">
        <property type="term" value="P:insulin processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043043">
        <property type="term" value="P:peptide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.250.3320:FF:000001">
        <property type="entry name" value="neuroendocrine convertase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000054">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.850:FF:000001">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000010">
        <property type="entry name" value="Putative neuroendocrine convertase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3320">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.850">
        <property type="entry name" value="Peptidase S8, pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022005">
        <property type="entry name" value="Proho_convert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032815">
        <property type="entry name" value="S8_pro-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038466">
        <property type="entry name" value="S8_pro-domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF14">
        <property type="entry name" value="NEUROENDOCRINE CONVERTASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12177">
        <property type="entry name" value="Proho_convert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16470">
        <property type="entry name" value="S8_pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="17" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 21 22" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 21 22" id="KW-0645">Protease</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 21 22" id="KW-0720">Serine protease</keyword>
    <keyword evidence="10 24" id="KW-0732">Signal</keyword>
    <keyword evidence="14" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040511553" description="Neuroendocrine convertase 1" evidence="24">
        <location>
            <begin position="28"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="25">
        <location>
            <begin position="460"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="622"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="629"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="643"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="20 21">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="20 21">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="20 21">
        <location>
            <position position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00015312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005028541.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="754" mass="84061" checksum="8CA78197FEA53CA3" modified="2023-09-13" version="2">MERRAWTLQCTAFALFCAWCALNSVKAKRQFVNEWAAEIPGGPEAASAIAEELGYDLLGQIGSLENHYLFKHKNHPRRSRRSAFHITKRLSDDDRVIWAEQQYEKERSKRSILRDSALNLFNDPMWNQQWYLQDTRMTAALPKLDLHVIPVWQKGITGKGVVITVLDDGLEWNHTDIYDNYDPEASYDFNDNDHDPFPRYDPTNENKHGTRCAGEIAMQANNHKCGVGVAYNSKVGGIRMLDGIVTDAIEASSIGFNPGHVDIYSASWGPNDDGKTVEGPGRLAQKAFEYGVKQGRQGKGSIFVWASGNGGRQGDNCDCDGYTDSIYTISISSASQQGLSPWYAEKCSSTLATSYSSGDYTDQRITSADLHNDCTETHTGTSASAPLAAGIFALALEANPNLTWRDMQHLVVWTSEYDPLANNPGWKKNGAGLMVNSRFGFGLLNAKALVDLAEPRTWSSVPEKKECVVKDNDFEPRALTANGEVIIEIPTRACEGQENAIKSLEHVQFEATIEYSRRGDLHVTLTSAAGTSTVLLAERERDTSPNGFKNWDFMSVHTWGENPIGTWTLRITDMSGRMQNEGRIVNWKLILHGTSSQPEHMKQPRVYTSYNTVQNDRRGVEKMVDSGEEQPTQENLTTNPLASKSPSGSSSVGGRRAELAEGAPSEAMLRLLQSAFSKNPAAQQSPKKSPSAKLNIPYENFYQALEKLNKPSQLKDSEDSLYNDYVDVFYNTKPYKHRDDRLLQALVDILREEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PTW3</accession>
    <name>A0A8C4PTW3_EQUAS</name>
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        <submittedName>
            <fullName evidence="4">WWC family member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">WWC3</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PTW3"/>
    <dbReference type="Ensembl" id="ENSEAST00005033124.2">
        <property type="protein sequence ID" value="ENSEASP00005030468.2"/>
        <property type="gene ID" value="ENSEASG00005020725.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00410000025556"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:kinase binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:signaling adaptor activity"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:cell migration"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="term" value="P:negative regulation of cell population proliferation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of hippo signaling"/>
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    </dbReference>
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        <property type="term" value="P:negative regulation of organ growth"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="C2_Kibra"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037771">
        <property type="entry name" value="C2_WWC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051105">
        <property type="entry name" value="WWC/KIBRA_Hippo_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057747">
        <property type="entry name" value="WWC1_hairpin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14791">
        <property type="entry name" value="BOMB/KIRA PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14791:SF25">
        <property type="entry name" value="PROTEIN WWC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25802">
        <property type="entry name" value="WWC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2" evidence="3">
        <location>
            <begin position="622"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="384"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="448"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="528"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="597"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="758"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="807"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="870"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="255"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="470"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="605"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="833"/>
            <end position="851"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030468.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1114" mass="125240" checksum="D8C25C4A1CB0FC34" modified="2023-09-13" version="2">MDHINKLTQIEDPREQWRREQERMLKEYLIVAQEALNAKKEIYQIKQQRFELAQEEYQQLHKMCEDDSRSYASSFSGYSTNTKYDPHQIKAEIASRRDRLSRLKRELTQMKQELQYKEKGVETLQEIDRKMSSAHTNYKLDEAQAIMSELRSIKKAICTGEKERRDLMQSLAKLTDGFKNSFSLTDPLQEFPHNPGALGDSLPQQFCDAGSQTDIIGEVRSPGGRAECAKREKGCFNVAITHLFVFDDKTRLVDRVRLNWQYEEARKRVSNIQQQLAQLDTESWPGMAEADRDRLQLIKEKEALLQELQLIVQQRRPAEDVARLEEERRRLEEEIQRARATSVQGATERILLQEKRNCLLMQLEEATRLTSYLQSQLKSLSASTLTVSSGSSRGSLASSRGSLASSRGSLSSVSFTDIYGLPQYEKPDTECGQLLRFDLIPFDSLGRDATFSDPPGPSGLHKQRRSLDTPQSLASLSSRSSLSSLSPPSSPLDTPFLPASRDSPLAQLGDSFEVSGLGTLDRLRAQASALGDEDSPGTASLQPHGFSGDGEGLRERGPQVAAIPTGATVTLREDSAKRLERRARRVSACLSDYSLASDSGVFEPPPKRSEDAEESARGDVCDNEGPQIHVGFLHDSASECLLVNVLQLRNFAGLVVKEDCKIHIRVYLPPLNSGMPNTYCSKALEFQAPLVFNEVFRIPVHSSMSMLKSLQLYVCSVNQQLQEELLGIAQINLADCDGPSELQLRWYSVQVFTSPEPPRARESVRAGEGSAWDPARAAPGKTDAVTVLLARTTAQLQAVERELAEEQAKLESTEEGVLEMARKEEQAEAASERSWQADSVDSGCSSCTQTSPPHPEPCCVGVDSIHGHTFAGQTDPYSPGKRQPPPLKVDKETNTEDLFPEEVASLPKERPSRRARGSPFVRSSTIVRSQTFSPGARSQYVCRLYRSDSDSSTLPRKSPFVRNTLERRTLRYKQSCRSSVAELMARTSLDLELDLQASRTRQRQLNEEICTLRELRQRLEDAQLRGQTDLPHWVLRDERFRSLLKEAERQTRQTKLDFHQEQAAEKMLKKASKGVCQLRGQNHKEPIQVQTFREKIAFFTRPRISIPPLPADDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PVQ9</accession>
    <name>A0A8C4PVQ9_EQUAS</name>
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        <recommendedName>
            <fullName evidence="12">Glucoside xylosyltransferase 1</fullName>
            <ecNumber evidence="11">2.4.2.42</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Glycosyltransferase 8 domain-containing protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">GXYLT1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Glycosyltransferase which elongates the O-linked glucose attached to EGF-like repeats in the extracellular domain of Notch proteins by catalyzing the addition of xylose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>3-O-(beta-D-glucosyl)-L-seryl-[EGF-like domain protein] + UDP-alpha-D-xylose = 3-O-[alpha-D-xylosyl-(1-&gt;3)-beta-D-glucosyl]-L-seryl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56064"/>
            <dbReference type="Rhea" id="RHEA-COMP:14610"/>
            <dbReference type="Rhea" id="RHEA-COMP:14611"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:140575"/>
            <dbReference type="ChEBI" id="CHEBI:140576"/>
            <dbReference type="EC" id="2.4.2.42"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.42" evidence="11"/>
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    <dbReference type="KEGG" id="eai:106845838"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
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    </evidence>
    <sequence length="440" mass="50543" checksum="BE7A7515F59A0096" modified="2023-09-13" version="2">MRRYLRIVGLCLACGFCSLLYAFSQLAVSLEEGAGGGGGKPQAGAASWLAGGGRGAAGGADSAGPAAHPGRSDRCKDFSLCYWNPYWMLPSDVCGMNCLWEAALRYNLKIQPVERMHLAVVACGERLEETITMLKSALIFSIKPLQFHIFAEDQLHDSFKGTLDSWSFLQTFNYSLYPITFPSENAVEWKKLFKPCASQRLFLPLILKEVDSLLYVDTDILFLRPVDDIWSLLKKFNSTQIAAMAPEHEEPRIGWYNRFARHPYYGKTGVNSGVMLMNMTRMRRKYFKNDMTTVRLRWGDILMPLLKKYKLNITWGDQDLLNIIFFHNPESLFVFPCQWNYRPDHCIYGSNCQEAEEEGIFILHGNRGVYHDDKQPAFRAIYEALRNCSFEDDNIRSLLKPLELELQKTVHTYCGKIYKIFIKQLAKSLRDRFDRPPKER</sequence>
</entry>
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    <name>A0A9L0I7E2_EQUAS</name>
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        <recommendedName>
            <fullName evidence="6">Cilia- and flagella-associated protein 263</fullName>
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        <name evidence="9" type="primary">CFAP263</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
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                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CFAP263 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0I7E2"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:cilium assembly"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="CC_CF"/>
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    <dbReference type="InterPro" id="IPR025254">
        <property type="entry name" value="CCDC113/CCDC96_CC"/>
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    <dbReference type="PANTHER" id="PTHR15654:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15654">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 113-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13870">
        <property type="entry name" value="CCDC113_CCDC96_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="3 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCDC113/CCDC96 coiled-coil" evidence="8">
        <location>
            <begin position="181"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="101"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="257"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005035721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="371" mass="43057" checksum="A7BDB48AAAD39331" modified="2023-09-13" version="1">MAGEACETPVSELQVEDENELPVIQLCGLVEELSYGNSALKTETEMFEKYYNKLEPRDQRLPPLSEIKISGAEFAQLRGRRRSKSRIGIDRALGLSVDQKCELVQKELEDTKEEIRHMRANAERDMQHHEAIIEEAEIRWAEVQREVHEFEKDVVKTISKKKGSILATQKVMKYIEDMNRRRDSIKDKLRLKNVSLKVQRKKVLSQLRQKEEVGEALHDVDFQQLKIENAQFLEAIETRNQELIQLKLASGNTLQVLNTYKSKLQRAMEMSSSLNKEILLRNELLEKIERETIQVEEDRAKAEALNKKLRKQLAEFRAPQVMGYVREKILNGDLEKSIKMWERKVEIAEGVCLGPSNLLLPRMFFLCHSGT</sequence>
</entry>
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    <accession>A0A9L0I847</accession>
    <name>A0A9L0I847_EQUAS</name>
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            <fullName evidence="5">Eukaryotic translation initiation factor 3 subunit M</fullName>
            <shortName evidence="5">eIF3m</shortName>
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        <name evidence="5 7" type="primary">EIF3M</name>
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    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
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                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CSN7/EIF3M family. CSN7 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eIF-3 subunit M family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044607623.1">
        <property type="nucleotide sequence ID" value="XM_044751688.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0I847"/>
    <dbReference type="SMR" id="A0A9L0I847"/>
    <dbReference type="Ensembl" id="ENSEAST00005068021.1">
        <property type="protein sequence ID" value="ENSEASP00005036672.1"/>
        <property type="gene ID" value="ENSEASG00005013531.2"/>
    </dbReference>
    <dbReference type="GeneID" id="123278586"/>
    <dbReference type="CTD" id="10480"/>
    <dbReference type="GeneTree" id="ENSGT00390000004456"/>
    <dbReference type="OMA" id="VCLKALW"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03012">
        <property type="entry name" value="eIF3m"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045237">
        <property type="entry name" value="COPS7/eIF3m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027528">
        <property type="entry name" value="eIF3m"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040750">
        <property type="entry name" value="eIF3m_C_helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350">
        <property type="entry name" value="COP9 SIGNALOSOME COMPLEX SUBUNIT 7/DENDRITIC CELL PROTEIN GA17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15350:SF2">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18005">
        <property type="entry name" value="eIF3m_C_helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="initiator methionine" description="Removed" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="domain" description="PCI" evidence="6">
        <location>
            <begin position="180"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="modified residue" description="N-acetylserine" evidence="5">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005036672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="374" mass="42503" checksum="63736CA2B093D794" modified="2023-09-13" version="1">MSVPAFIDISEEDQAAELRAYLKSKGAEISEENSEGGLHVDLAQIIEACDVCLKEDDKDVESVMNSVVSLLLILEPDKQEALIESLCEKLVKFREGERPSLRLQLLSNLFHGMDKNTPVRYTVYCSLIKVAASCGAIQYIPTELDQVRKWISDWNLTTEKKHTLLRLLYEALVDCKKSDAASKVMVELLGSYTEDNASQARVDAHRCIVRALKDPNAFLFDHLLTLKPVKFLEGELIHDLLTIFVSAKLASYVKFYQNNKDFIDSLGLLHEQNMAKMRLLTFMGMAVENKEISFDTMQQELQIGADDVEAFVIDAVRTKMVYCKIDQTQRKVVVSHSTHRTFGKQQWQQLYDTLNAWKQNLNKVKNSLLSLSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IBJ9</accession>
    <name>A0A9L0IBJ9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">G protein subunit alpha o1</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Forms a complex with GNB1 and GNG3. Interacts with RGS14. Interacts with RGS16. Interacts with RGS19. Interacts (when palmitoylated) with ADGRG3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the G-alpha family. G(i/o/t/z) subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IBJ9"/>
    <dbReference type="Ensembl" id="ENSEAST00005049753.1">
        <property type="protein sequence ID" value="ENSEASP00005038247.1"/>
        <property type="gene ID" value="ENSEASG00005028877.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155883"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051430">
        <property type="term" value="F:corticotropin-releasing hormone receptor 1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031821">
        <property type="term" value="F:G protein-coupled serotonin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031852">
        <property type="term" value="F:mu-type opioid receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007188">
        <property type="term" value="P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007212">
        <property type="term" value="P:G protein-coupled dopamine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00066">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002307">
        <property type="entry name" value="Guanine nucleotide-binding protein G(k) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000020">
        <property type="entry name" value="Guanine nucleotide-binding protein G(o) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001408">
        <property type="entry name" value="Gprotein_alpha_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218:SF363">
        <property type="entry name" value="G PROTEIN SUBUNIT ALPHA O1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10218">
        <property type="entry name" value="GTP-BINDING PROTEIN ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00318">
        <property type="entry name" value="GPROTEINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00441">
        <property type="entry name" value="GPROTEINAI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51882">
        <property type="entry name" value="G_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0519">Myristate</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="125"/>
            <end position="126"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="150"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="175"/>
            <end position="179"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="244"/>
            <end position="247"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="328" mass="38124" checksum="0D0B588F13F6E268" modified="2023-09-13" version="1">MAERRPPTQETQHSTRPWSRQEKRKRDVRRKFTISCRLLCREQTETVKVGLKEEVWDGPARFTATSHRWDSPLSQADAKMVCDVVSRMEDTEPFSAELLSAMMRLWGDSGIQECFNRSREYQLNDSAKYYLDSLDRIGAADYQPTEQDILRTRVKTTGIVETHFTFKNLHFRLFDVGGQRSERKKWIHCFEDVTAIIFCVALSGYDQVLHEDETTNRMHESLKLFDSICNNKWFTDTSIILFLNKKDIFEEKIKKSPLTICFPEYTGPSAFTEAVAYIQAQYESKNKSTHKEIYTHVTCATDTNNIQFVFDAVTDVIIAKNLRGCGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IDA4</accession>
    <name>A0A9L0IDA4_EQUAS</name>
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        <recommendedName>
            <fullName evidence="24">Voltage-dependent L-type calcium channel subunit alpha</fullName>
        </recommendedName>
    </protein>
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        <name evidence="28" type="primary">CACNA1F</name>
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    <organism evidence="28 29">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1F gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, and by benzothiazepines. Activates at more negative voltages and does not undergo calcium-dependent inactivation (CDI), due to incoming calcium ions, during depolarization.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="21">Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. Interacts (via IQ domain) with CABP4; in a calcium independent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 24">Membrane</location>
            <topology evidence="1 24">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1F subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSEASP00005035755.1"/>
        <property type="gene ID" value="ENSEASG00005037675.1"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="term" value="C:voltage-gated calcium channel complex"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050908">
        <property type="term" value="P:detection of light stimulus involved in visual perception"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="InterPro" id="IPR050599">
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    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
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    <keyword evidence="9 22 24" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 24" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="3 24" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15 23" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="6 26" id="KW-0812">Transmembrane</keyword>
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    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="17" id="KW-0844">Vision</keyword>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00054074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00061406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00062383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU003808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="SMART" id="SM01062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005035755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1974" mass="220089" checksum="8D0BA4A68E4709DF" modified="2023-09-13" version="1">MSASEGGKDTTPEPSPANGAGPGPEWGLCPEPPASGGETGGASGLGTPKRRTQHSKHKTVAVASAQRSPRALFCLTLANPLRRSCISIVEWKPFDILILLTIFANCVALGVYIPFPEDDSNTANHNLEQVEYVFLVIFTVETVLKIVAYGLVLHPSAYIRNGWNLLDFIIVVVGLFSVLLEQGPGRPGDAPHTGGKPGGFDVKALRAFRVLRPLRLVSGVPSLHIVLNSIMKALVPLLHIALLVLFVIIIYAIIGLELFLGRMHKTCYFLGSDVEAEEDPSPCASSGSGRACTLNQTECRGRWAGPNGGITNFDNFFFAMLTVFQCVTMEGWTDVLYWMQDAMGYELPWLYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLRGYLDWITQAEELDIEDPSADGSVVEEGRAGHRPQLAELTNRRRGHLRWFSHSTRSTHSTSSHASLPASDTGSMAETPGDEEEEEGALASCTLCLNKIMKTRVCRRLRRANRGLRARCRRAVKSNTCYWAVLLLVFLNTLTIASEHHGQPVWLTQTQEYANKVLLCLFTVEMLLKLYGLGPSTYVSSFFNRFDCFVVCGGILETTLVEVGAMQPLGISVLRCVRLLRIFKVTRHWASLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDQTHTKRSTFDTFPQALLTVFQILTGEDWNVVMYDGIMAYGGPFFPGMLVCVYFIILFICGNYILLNVFLAIAVDNLASGDTGTEKNKGREKSTEENPHQENGELVSGGENEEEEGAKNEGADMEEEEEEEEEEEEQEEGGAGRVELLQEVVPKEKVVPIPEGSAFFCLSQTNPLRRACHALIHHHVFTNLILVFIILSSVSLAAEDPIRAHSFRNHILGYFDYAFTSIFTVEILLKMTVFGAFLHRGSFCRNWFNLLDLLVVSVSLISFGIHSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFACIGVQLFKGKFYSCTDEAKHTPQECKGSFLVYPDGDVSRPLVRERLWVNSDFNFDNVLSAMMALFTVSTFEGWPALLYKAIDAHAEDKGPIYNYRVEISVFFIVYIIIIAFFMMNIFVGFVIITFRAQGEQEYQNCELDKNQRQCVEYALKAQPLRRYIPKNPHQYRVWATVNSAAFEYLMFLLILLNTVALAMQHYEQTAPFNYAMDILNMVFTGLFTVEMVLKIIAFKPKHYFTDAWNTFDALIVVGSIVDIAVTEVNNGGHLGESSEDSSRISITFFRLFRVMRLVKLLSKGEGIRTLLWTFIKSFQALPYVALLIAMIFFIYAVIGMQMFGKVALQDGTQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLASLPGSRCDPESDFSPGEEFTCGSNFAIAYFISFFMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPGAKGRIKHLDVVALLRRIQPPLGFGKLCPHRVACKRLVAMNMPLNSDGTVTFNATLFALVRTSLKIKTEGNLEQANQELRIVIKKIWKRMKQKLLDEVIPPADEEEVTVGKFYATFLIQDYFRKFRRRKEKGLLGTEAPPSTSSALQAGLRSLQDLGPEIRQALTCDTDEEEEEEGQEGEEEEDEKDSQTYKASMGSQPPSRRSSVISVSLPAGDRLPDSLSLAPSDDDGGAPNSRQSSVPQTGSHTHRRGSGVLIFTIPEEGSSQPKGTKGQEKQDEEEEVPSQLSYLDEQEGTALRPILLPPHRPQRYVDGHHVPRRRLLPPTPAGRKPSFTIQCLRRQGSCEDLPIPGTYHRGRNSGPSRAQGSWATPPQRGRLLYAPLLLVEEGAAGEGYLGKSSGPLCTFTCLHVPGTHSDPSHGKRGSADSLVEAVLISEGLGLFARDPRFVALAKQEIADACRLTLDEMDSAASDLLAQGTSSLYSDEESILSRFDEEDLGDEMACVHAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IH74</accession>
    <name>A0A9L0IH74_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glucose-6-phosphatase</fullName>
            <ecNumber evidence="11">3.1.3.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">G6PC3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. May form with the glucose-6-phosphate transporter (SLC37A4/G6PT) a ubiquitously expressed complex responsible for glucose production through glycogenolysis and gluconeogenesis. Probably required for normal neutrophil function.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the glucose-6-phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.9" evidence="11"/>
    <dbReference type="RefSeq" id="XP_044600700.1">
        <property type="nucleotide sequence ID" value="XM_044744765.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IH74"/>
    <dbReference type="Ensembl" id="ENSEAST00005039284.1">
        <property type="protein sequence ID" value="ENSEASP00005039527.1"/>
        <property type="gene ID" value="ENSEASG00005010158.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106836447"/>
    <dbReference type="KEGG" id="eai:106836447"/>
    <dbReference type="CTD" id="92579"/>
    <dbReference type="GeneTree" id="ENSGT00950000183150"/>
    <dbReference type="OMA" id="KKWCSRA"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004346">
        <property type="term" value="F:glucose-6-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015760">
        <property type="term" value="P:glucose-6-phosphate transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03381">
        <property type="entry name" value="PAP2_glucose_6_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000018">
        <property type="entry name" value="Glucose-6-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016275">
        <property type="entry name" value="Glucose-6-phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12591">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12591:SF2">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000905">
        <property type="entry name" value="Glucose-6-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 11" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="285"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="308"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="15">
        <location>
            <begin position="53"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00055989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000905-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000905-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="346" mass="38678" checksum="899DB49362C31206" modified="2023-09-13" version="1">MESAMSAGIAVAEALQNQLPWLENVWLWVNFLGDPKSLFLFYFPAAYYASRRVGIAVLWISLIAEWLNLVFKWLLFGDRPFWWVHESGYYSQAPAQVHQFPSSCETGPGSPSGHCMITGAALWPIMTAISSQMATRTHSRWVRMIPSLAYITFLLAVGLSRVFLLAHFPHQVLAGLITGAVLGWLMAPQVPMERELSFYGLTSLALLLGTSLIYWTLFTLGLDLSWSISLASKWCERPEWVHVDTRPFASLSRDSGSALGLGIALHSPCYAQVRRAYLGNGQKIACFVLAMGLLGPLDWLGYTPQISIFYIVNFLKYTLWPCLVLALVPWVVHMFSAQEAPPIRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IHZ9</accession>
    <name>A0A9L0IHZ9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DAZ-associated protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Deleted in azoospermia-associated protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proline-rich transcript in brain protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">DAZAP2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
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                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="37" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="37" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24 39">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="25">Heterodimer and heterotetramer with ligand GAS6. Interacts with CBL, GRB2, LCK, NCK2, PIK3R1, PIK3R2, PIK3R3, PLCG1, SOCS1 and TNS2. Part of a complex including AXL, TNK2 and GRB2, in which GRB2 promotes AXL recruitment by TNK2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014861013.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A9L0IJU8"/>
    <dbReference type="Ensembl" id="ENSEAST00005073838.1">
        <property type="protein sequence ID" value="ENSEASP00005041163.1"/>
        <property type="gene ID" value="ENSEASG00005006349.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106843809"/>
    <dbReference type="CTD" id="558"/>
    <dbReference type="GeneTree" id="ENSGT00940000160232"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004714">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001779">
        <property type="term" value="P:natural killer cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001818">
        <property type="term" value="P:negative regulation of cytokine production"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006909">
        <property type="term" value="P:phagocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030168">
        <property type="term" value="P:platelet activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051249">
        <property type="term" value="P:regulation of lymphocyte activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd20966">
        <property type="entry name" value="IgI_1_Axl_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05075">
        <property type="entry name" value="PTKc_Axl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000865">
        <property type="entry name" value="AXL receptor tyrosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000089">
        <property type="entry name" value="Tyrosine-protein kinase receptor TYRO3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000662">
        <property type="entry name" value="Tyrosine-protein kinase receptor UFO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000810">
        <property type="entry name" value="Tyrosine-protein kinase receptor UFO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000509">
        <property type="entry name" value="Tyrosine-protein kinase receptor UFO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000696">
        <property type="entry name" value="tyrosine-protein kinase receptor UFO isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF323">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR UFO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 28 30" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0221">Differentiation</keyword>
    <keyword evidence="20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="22" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0391">Immunity</keyword>
    <keyword evidence="23" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="29" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18 32" id="KW-0472">Membrane</keyword>
    <keyword evidence="29" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 28 30" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-0675">Receptor</keyword>
    <keyword evidence="38" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 33" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 32" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17 32" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="19" id="KW-0829">Tyrosine-protein kinase</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="33">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040380337" description="Tyrosine-protein kinase receptor UFO" evidence="33">
        <location>
            <begin position="21"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="434"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="35">
        <location>
            <begin position="20"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="35">
        <location>
            <begin position="132"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="36">
        <location>
            <begin position="220"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="36">
        <location>
            <begin position="329"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="34">
        <location>
            <begin position="520"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="810"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="852"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="27">
        <location>
            <position position="656"/>
        </location>
    </feature>
    <feature type="binding site" evidence="30">
        <location>
            <position position="551"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="28">
        <location>
            <position position="660"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="661"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <position position="674"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00051243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00064126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00068587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PIRSR" id="PIRSR000615-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="36">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005041163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="38">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="878" mass="96489" checksum="58B897027344E0A7" modified="2023-09-13" version="1">MGRVPLVWCLVLCCWGCVTPKGTQAEANPFVGSLGNITGARGLMGALRCELQVQGEPPEVTWLRDGQVLELADSTQTQVPLGEDGQDDWKVVSQLRISSLQLSDAGWYQCAVVLGGKTFVSQPGYVGLEGLPYFLEEPEDRAVTANTPFNLSCRAQGPPEPVDLLWLQDTVSLAPATGHGPQHMLRVPGLNKTSSFSCEAHNAKGVTTSRTATIKVLPQRPRDLHLVSSQPTELEVAWTPGLSGIYPLTHCTLQAVLSDDGVGIWLGEPDPPEEPLTLQASVPPHRIRLGSLHPHTPYHIRVACASTQGPSPWTHWLPVETSEGVPLGPPENVSAMRNGSQALVRWQEPRAPLQGTLLGYRLAYRGQDTPEVLMDIGLKREVTLELRGDGTVPNLTVCVAAYTAAGDGPWSLPVLLEPWRPVSEPPAPAFSWPWWYVLLGAVVAAACVLILALFLVHRRKKETRYGEVFEPTVERGELVVRYRVRKSYSRRTTEATLNSLGISEELKEKLRDVMVDRHKVALGKTLGEGEFGAVMEGQLNQDDSILKVAVKTMKIAICTRSELEDFLSEAVCMKEFDHPNVMRLIGVCFQGSEREGFPAPVVILPFMKHGDLHSFLLYSRLGDQPVFLPTQMLVKFMADIASGMEYLSTKRFIHRDLAARNCMLNENMSVCVADFGLSKKIYNGDYYRQGRIAKMPVKWIAIESLADRVYTSKSDVWSFGVTMWEIATRGQTPYPGVENSEIYDYLRQGNRLKQPVDCLDGLYVLMSRCWELNPRDRPSFAELREDLENTLKALPPAQEPDEILYVNMDEGVGHPEPLGAAGGADPPTQPDPKDSCSCLTAAEVHPAGRYVLCPSTAPGPTLPADRGSPASPGQEDGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IJW9</accession>
    <name>A0A9L0IJW9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 19">Calpain-3</fullName>
            <ecNumber evidence="13 19">3.4.22.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">CAPN3</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Calcium-regulated non-lysosomal thiol-protease. Proteolytically cleaves CTBP1. Mediates, with UTP25, the proteasome-independent degradation of p53/TP53.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 19">
            <text>Broad endopeptidase activity.</text>
            <dbReference type="EC" id="3.4.22.54"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer; via EF-hand domain 4. Interacts with TTN/titin. Interacts with CMYA5; this interaction, which results in CMYA5 proteolysis, may protect CAPN3 from autolysis. Interacts with SIMC1. Interacts with UTP25; the interaction is required for CAPN3 translocation to the nucleolus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the peptidase C2 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.54" evidence="13 19"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0IJW9"/>
    <dbReference type="Ensembl" id="ENSEAST00005054505.1">
        <property type="protein sequence ID" value="ENSEASP00005038060.1"/>
        <property type="gene ID" value="ENSEASG00005013499.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156092"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00214">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16190">
        <property type="entry name" value="EFh_PEF_CAPN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000555">
        <property type="entry name" value="Calpain-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000065">
        <property type="entry name" value="calpain-3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.380:FF:000002">
        <property type="entry name" value="calpain-3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033883">
        <property type="entry name" value="C2_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022682">
        <property type="entry name" value="Calpain_domain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022683">
        <property type="entry name" value="Calpain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032100">
        <property type="entry name" value="Calpain_u2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054069">
        <property type="entry name" value="CAPN3/13-like_C_EFh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029531">
        <property type="entry name" value="CAPN3_PEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF329">
        <property type="entry name" value="CALPAIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01067">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16648">
        <property type="entry name" value="Calpain_u2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21875">
        <property type="entry name" value="CAPN13-like_C_EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00720">
        <property type="entry name" value="calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 19" id="KW-0106">Calcium</keyword>
    <keyword evidence="4 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 18 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 18 19" id="KW-0645">Protease</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 18 19" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="21">
        <location>
            <begin position="74"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="22">
        <location>
            <begin position="716"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="22">
        <location>
            <begin position="781"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="607"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="616"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="active site" evidence="17 18">
        <location>
            <position position="129"/>
        </location>
    </feature>
    <feature type="active site" evidence="17 18">
        <location>
            <position position="334"/>
        </location>
    </feature>
    <feature type="active site" evidence="17 18">
        <location>
            <position position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00057973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU367132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="815" mass="93921" checksum="41A50FB160FE7CDE" modified="2023-09-13" version="1">MPTVISASVAPRTGAEPMSPGPVAHPAQGSTTESGGGNPSGNYSAIISRNFPIIGVKEKTFEKLHKKCLEKNVLYVDPEFPPDETSLFYSQKFPIQFVWKRPPEICKNPRFIIGGANRTDICQGDLGDCWFLAAIACLTLNERLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFFEIKEAPRDMYRIMKKAIERGSLMGCSIDDGTNMTYGTSPSGLKMGDLIARMVRNMDNSRLRDSDLIPRDSDDRPTRTIVPVQYETRMACGLVRGHAYSVIGLEEAWFKNEKVKLVRLRNPWGQVEWNGSWSDGWKDWSFVDKEEKARLQHQVTEDGEFWMSYDDFIYHFTKLEICNLTADALESDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPEDSEVICSFLVALMQKNRRKNRKLGANLFTIGFAIYEVPKEMHGNRQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPVPIIFVSDRANSNKELGVDQESEEGKDKTSPDKQERSPQPQPGITDQESEEQQQFYNIFRQIAGDDMEISADELKNILNTVVNRHKDLKTQGFTLESCRSMIALMDTDGSGRLNLQEFHHLWNKIKTWQKIFKHYDTDQSGTINSYEMRNAVNDAGFHLNRQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IL48</accession>
    <name>A0A9L0IL48_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Plasminogen activator</fullName>
            <ecNumber evidence="13">3.4.21.68</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">PLAT</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.</text>
            <dbReference type="EC" id="3.4.21.68"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the peptidase S1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.68" evidence="13"/>
    <dbReference type="RefSeq" id="XP_014693260.1">
        <property type="nucleotide sequence ID" value="XM_014837774.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IL48"/>
    <dbReference type="Ensembl" id="ENSEAST00005048043.1">
        <property type="protein sequence ID" value="ENSEASP00005037793.1"/>
        <property type="gene ID" value="ENSEASG00005012551.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106828955"/>
    <dbReference type="KEGG" id="eai:106828955"/>
    <dbReference type="CTD" id="5327"/>
    <dbReference type="GeneTree" id="ENSGT00940000158930"/>
    <dbReference type="OMA" id="WCYIFKA"/>
    <dbReference type="OrthoDB" id="6020543at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071396">
        <property type="term" value="P:cellular response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901701">
        <property type="term" value="P:cellular response to oxygen-containing compound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031639">
        <property type="term" value="P:plasminogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048008">
        <property type="term" value="P:platelet-derived growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014909">
        <property type="term" value="P:smooth muscle cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00061">
        <property type="entry name" value="FN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00108">
        <property type="entry name" value="KR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000043">
        <property type="entry name" value="Plasminogen activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000483">
        <property type="entry name" value="Tissue-type plasminogen activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.20.10:FF:000001">
        <property type="entry name" value="Urokinase-type plasminogen activator"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.20.10">
        <property type="entry name" value="Plasminogen Kringle 4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000083">
        <property type="entry name" value="Fibronectin_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000001">
        <property type="entry name" value="Kringle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013806">
        <property type="entry name" value="Kringle-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018056">
        <property type="entry name" value="Kringle_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038178">
        <property type="entry name" value="Kringle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050127">
        <property type="entry name" value="Serine_Proteases_S1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026280">
        <property type="entry name" value="Tissue_plasm_act"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264:SF42">
        <property type="entry name" value="TISSUE-TYPE PLASMINOGEN ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24264">
        <property type="entry name" value="TRYPSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00039">
        <property type="entry name" value="fn1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00051">
        <property type="entry name" value="Kringle"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001145">
        <property type="entry name" value="Tissue_plasm_act"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00018">
        <property type="entry name" value="KRINGLE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00058">
        <property type="entry name" value="FN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00130">
        <property type="entry name" value="KR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57440">
        <property type="entry name" value="Kringle-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01253">
        <property type="entry name" value="FN1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51091">
        <property type="entry name" value="FN1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00021">
        <property type="entry name" value="KRINGLE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50070">
        <property type="entry name" value="KRINGLE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 16 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 17" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 18" id="KW-0420">Kringle</keyword>
    <keyword evidence="12 13" id="KW-0617">Plasminogen activation</keyword>
    <keyword evidence="6 13 19" id="KW-0645">Protease</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 13 19" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040430533" description="Plasminogen activator" evidence="20">
        <location>
            <begin position="30"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-I" evidence="24">
        <location>
            <begin position="42"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="21">
        <location>
            <begin position="85"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="22">
        <location>
            <begin position="129"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Kringle" evidence="22">
        <location>
            <begin position="218"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="23">
        <location>
            <begin position="315"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Important for binding to annexin A2" evidence="15">
        <location>
            <begin position="45"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="14">
        <location>
            <position position="361"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="14">
        <location>
            <position position="410"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="14">
        <location>
            <position position="517"/>
        </location>
    </feature>
    <feature type="site" description="Important for binding to LRP1" evidence="15">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="site" description="Important for single-chain activity" evidence="15">
        <location>
            <position position="468"/>
        </location>
    </feature>
    <feature type="site" description="Important for single-chain activity" evidence="15">
        <location>
            <position position="516"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="44"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="89"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16 17">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16 17">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="130"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="151"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="182"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="219"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="240"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="271"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="disulfide bond" description="Interchain (between A and B chains)" evidence="16">
        <location>
            <begin position="303"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="346"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="354"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="448"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="480"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="513"/>
            <end position="541"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR001145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001145-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001145-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001145-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005037793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="566" mass="63974" checksum="190723A1E6CD8B46" modified="2023-09-13" version="1">MKNKMKRELLCVLLLCGALFILPSQETHTHTLFRRGARSYRVTCRDEKTQMTYRQHESWLRPMLRGNRVEYCWCDSGRPQCHSVPVKGCSEPRCFNGGTCWQALYFSDFVCQCPEGFVGKRCEIDASATCYEDQGVTYRGTWSTAESGSECVNWNSSVLALKPYNGRRPDAIRLGLGNHNYCRNPDQDSKPWCYVFKAGKYSSEPCSTPSCSKEKNGDCYTGRGLAYRGTHSLTTSGSSCLQWNSMTLIGKVYTAWMTNAQALGLGKHNYCRNPDGDAKPWCHVLKDRKLTWEYCDVPQCSTCGLRQYTQPQFRIKGGLYADIASHPWQAAIFAKNRKTPGERFLCGGILISSCWLLSAAHCFQERFPPHHLKVVLGRTYRLVPGEEEQKFEVEKYIVHQEFDDDTYDNDIALLQLKSDSMQCAQESDTVRTVCLPEADLQLPDWTECELSGYGKHEATSPFYSERLKEAHVRLYPASRCTSQYLSNRTITDNMLCAGDTRTGGNQANLHDACQGDSGGPLVCMQDNHMTLVGIISWGLGCGRKDVPGVYTKVTNYLAWIQDNMRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0ILM2</accession>
    <name>A0A9L0ILM2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Serine/threonine-protein kinase VRK1</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Vaccinia-related kinase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">VRK1</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with HDAC1, KAT2B, SETDB1, KDM3A and KDM4A. Associates with the nucleosome through interactions with nucleosome DNA, histone H2A and histone H2B; the interaction with H2A and H2B is mediated by the nucleosome acidic patch, a cluster of negatively charged residues of H2A and H2B forming a cleft within the nucleosome core.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Cajal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="RefSeq" id="XP_070370383.1">
        <property type="nucleotide sequence ID" value="XM_070514282.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0ILM2"/>
    <dbReference type="Ensembl" id="ENSEAST00005074806.1">
        <property type="protein sequence ID" value="ENSEASP00005038675.1"/>
        <property type="gene ID" value="ENSEASG00005021560.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106832620"/>
    <dbReference type="CTD" id="7443"/>
    <dbReference type="GeneTree" id="ENSGT00940000155554"/>
    <dbReference type="OMA" id="MHSTGYV"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14122">
        <property type="entry name" value="STKc_VRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000348">
        <property type="entry name" value="serine/threonine-protein kinase VRK1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 20 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8 20 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 21" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="37"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="360"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="375"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00061699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00062338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00073147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00081230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="396" mass="45365" checksum="C3D8F572475673F3" modified="2023-09-13" version="1">MPRVKAAQAVRQGPAKRRLAEHFAAGEIITDLTKKEWKLGLPVGQGGFGCIYLADMNSSKSVGNDAPCVVKVEPSDNGPLFTELKFYQRAAKPEQIQKWIRTHKLKYLGVPKYWGSGLHDKNGKSYRFMIMDRFGSDLQKIYEANAKRFSRKTVLQLSLRILDILEYIHEHEYVHGDIKASNLLLSHKNPDQVYLVDYGLAYRYCPEGIHKEYKEDPKRCHDGTIEFTSIDAHNGVAPSRRGDLEILGYCMIQWLSGHLPWEDNLKDPNYVRDSKIRYRENIAGLMDKCFPEKNKPDEIAKYMETVKLLDYAEKPLYQNLRDILLQGLKAIGSKDDGKLDFSVVENGGLKTKTITKKRKKEIEGSTESIVEDMECSNTQTEEATQTRSKTRKRVQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IMA5</accession>
    <name>A0A9L0IMA5_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Phosphoinositide interacting regulator of transient receptor potential channels</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PIRT</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
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                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Yang"/>
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                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="Zhang"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="MBDa"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyl-CpG-binding" evidence="3">
        <location>
            <begin position="28"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-CpG binding protein 2/3 C-terminal" evidence="2">
        <location>
            <begin position="102"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16564"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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        </source>
    </evidence>
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            <fullName evidence="2">26S proteasome complex subunit SEM1</fullName>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Wu"/>
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                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
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    <comment type="similarity">
        <text evidence="1 2">Belongs to the DSS1/SEM1 family.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007834">
        <property type="entry name" value="DSS1_SEM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16771">
        <property type="entry name" value="26 PROTEASOME COMPLEX SUBUNIT DSS1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR16771:SF0">
        <property type="entry name" value="26S PROTEASOME COMPLEX SUBUNIT SEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05160">
        <property type="entry name" value="DSS1_SEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DSS1_SEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005042789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="70" mass="8202" checksum="72528CA40DEE24A5" modified="2023-09-13" version="1">PLSRPCTHDLLCDQRWVRSVTVAVPDWAGLDEDEDAHVWEDNWDDDNVEDDFSNQLRAELEKHGYKMETS</sequence>
</entry>
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    <accession>A0A9L0ISL8</accession>
    <name>A0A9L0ISL8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TNF receptor-associated factor</fullName>
        </recommendedName>
    </protein>
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        <name evidence="11" type="primary">TRAF1</name>
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        <name type="scientific">Equus asinus</name>
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        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Zhang"/>
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                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Adapter molecule that regulates the activation of NF-kappa-B and JNK. Plays a role in the regulation of cell survival and apoptosis. The heterotrimer formed by TRAF1 and TRAF2 is part of a E3 ubiquitin-protein ligase complex that promotes ubiquitination of target proteins, such as MAP3K14. The TRAF1/TRAF2 complex recruits the antiapoptotic E3 protein-ubiquitin ligases BIRC2 and BIRC3 to TNFRSF1B/TNFR2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TNF receptor-associated factor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044634862.1">
        <property type="nucleotide sequence ID" value="XM_044778927.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_044778928.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_044778929.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044634865.1">
        <property type="nucleotide sequence ID" value="XM_044778930.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070374958.1">
        <property type="nucleotide sequence ID" value="XM_070518857.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_070518858.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070374960.1">
        <property type="nucleotide sequence ID" value="XM_070518859.1"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_070518860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0ISL8"/>
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    <dbReference type="Ensembl" id="ENSEAST00005057909.1">
        <property type="protein sequence ID" value="ENSEASP00005040074.1"/>
        <property type="gene ID" value="ENSEASG00005021967.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106824204"/>
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    <dbReference type="GeneTree" id="ENSGT00940000161076"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031996">
        <property type="term" value="F:thioesterase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043122">
        <property type="term" value="P:regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001236">
        <property type="term" value="P:regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03779">
        <property type="entry name" value="MATH_TRAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000053">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000001">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012227">
        <property type="entry name" value="TNF_rcpt-assoc_TRAF_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049342">
        <property type="entry name" value="TRAF1-6_MATH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037306">
        <property type="entry name" value="TRAF1_MATH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032070">
        <property type="entry name" value="TRAF_BIRC3-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF96">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21355">
        <property type="entry name" value="TRAF-mep_MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16673">
        <property type="entry name" value="TRAF_BIRC3_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015614">
        <property type="entry name" value="TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MATH" evidence="10">
        <location>
            <begin position="265"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="186"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR015614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005040074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="415" mass="45742" checksum="B65C3649180181C1" modified="2023-09-13" version="1">MASGSASSPRPAPDENEFPFGCPPTVCEDPSEPRALCCAACLSENSRNGEDRVCLKCRGDDSQSVGPGSLVSQEKDLPEVAEAGVGCPFAGVGCSFKGSPKFMQEHEVASQATHLNLVLGFMKQWKAQLGSGLGSGPMALEQNLSDLQLQAAVEVAGDLEVDCYRAPCSESQEELALQHFMKEKLLAELEGKLRVFENIVAVLNKEVEASHLALAASIHQSQLDREHILSLEQRVLELQQTLAQKDQALGKLEQSLRLMEEASFDGTFLWKITNVTRRCHESACGRTVSLFSPAFYTAKYGYKLCLRLYLNGDGTGKRTHLSLFIVIMRGEYDALLPWPFRNKVTFMLIDQNNREHAIDAFRPDLNSASFQRPQSETNVASGCPLFFPLSRLQSPKHAYVKDDTMFLKCIVETSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IUR3</accession>
    <name>A0A9L0IUR3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C-X-C motif chemokine</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CXCL11</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the intercrine alpha (chemokine CxC) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014721299.1">
        <property type="nucleotide sequence ID" value="XM_014865813.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IUR3"/>
    <dbReference type="Ensembl" id="ENSEAST00005049160.1">
        <property type="protein sequence ID" value="ENSEASP00005043848.1"/>
        <property type="gene ID" value="ENSEASG00005024870.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106846798"/>
    <dbReference type="KEGG" id="eai:106846798"/>
    <dbReference type="CTD" id="6373"/>
    <dbReference type="GeneTree" id="ENSGT00530000064263"/>
    <dbReference type="OMA" id="ANKGQRC"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008009">
        <property type="term" value="F:chemokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048248">
        <property type="term" value="F:CXCR3 chemokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070098">
        <property type="term" value="P:chemokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042119">
        <property type="term" value="P:neutrophil activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030593">
        <property type="term" value="P:neutrophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051281">
        <property type="term" value="P:positive regulation of release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010818">
        <property type="term" value="P:T cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00273">
        <property type="entry name" value="Chemokine_CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.40:FF:000004">
        <property type="entry name" value="C-X-C motif chemokine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039809">
        <property type="entry name" value="Chemokine_b/g/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001089">
        <property type="entry name" value="Chemokine_CXC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018048">
        <property type="entry name" value="Chemokine_CXC_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033899">
        <property type="entry name" value="CXC_Chemokine_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015:SF191">
        <property type="entry name" value="C-X-C MOTIF CHEMOKINE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00048">
        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00437">
        <property type="entry name" value="SMALLCYTKCXC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00199">
        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00471">
        <property type="entry name" value="SMALL_CYTOKINES_CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="4 8" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040537203" description="C-X-C motif chemokine" evidence="8">
        <location>
            <begin position="22"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="9">
        <location>
            <begin position="27"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005043848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="100" mass="11042" checksum="49E0A1EF1A5F3F20" modified="2023-09-13" version="1">MCVKGMSTVLAVIFCAMIVQGFPMFKGGRCLCIGPGVKAVKVADIEKVSIIYPSNNCDKIELIITLKAHKGQRCLNPKSKQANAIIKKAERRNFLKYQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J426</accession>
    <name>A0A9L0J426_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Zinc finger protein GLIS2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">GLI-similar 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Neuronal Krueppel-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">GLIS2</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A9L0J426"/>
    <dbReference type="Ensembl" id="ENSEAST00005039474.1">
        <property type="protein sequence ID" value="ENSEASP00005047123.1"/>
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        <property type="component" value="Chromosome 14"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:nervous system development"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000019">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000310">
        <property type="entry name" value="GLIS family zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000357">
        <property type="entry name" value="GLIS family zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GLIS family zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000532">
        <property type="entry name" value="GLIS family zinc finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF8">
        <property type="entry name" value="GLIS FAMILY ZINC FINGER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="16" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 21" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="202"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="23">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="263"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="23">
        <location>
            <begin position="293"/>
            <end position="317"/>
        </location>
    </feature>
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        <location>
            <begin position="39"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="84"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
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            <end position="487"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="459"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022782"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
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        </source>
    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005047123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="524" mass="55682" checksum="A7553DF3A4F2F35A" modified="2023-09-13" version="1">MHSLDEPLDLKLSITKLRAAREKRERTLSAVRHRALHRELGLVDDSPTPGSPGSPPSGFLLNPKFPEKVEGRFSAAPLVDLSLSPPSGLDSPNGSSSLSPERQGNGDLPAVPTAPDFQPLRYLDGVPSSFQFFLPLGSGGALHLPASSFLTPAKDKCLSPELPLPKQLVCRWAKCNQLFELLQDLVDHVNDYHVKPEKDAGYCCHWEGCARHGRGFNARYKMLIHIRTHTNEKPHRCPTCSKSFSRLENLKIHNRSHTGEKPYVCPYEGCNKRYSNSSDRFKHTRTHYVDKPYYCKMPGCHKRYTDPSSLRKHIKAHGHFVSHEQQELLQLRPPPKPPLPTPDGSPYVSGAQIIIPNPAALFGGPGLPGLPLPLAPGPLDLSALACANGGGGGGAGGMGPGLPGPVLPLNLAKNPLLPSPFGAGGLGLPVVSLLAGSAGGKAEGEKGCGAGSARALGVEGHKTPLERTESSHSRPSPDGLPLLPGTVLDLSTGVNSAASSPEALTPGWVVIPPGSVLLKPAVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J4J5</accession>
    <name>A0A9L0J4J5_EQUAS</name>
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        <submittedName>
            <fullName evidence="9">Neuropeptide Y</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NPY</name>
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    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="Liu"/>
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                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasmic vesicle</location>
            <location evidence="6">Secretory vesicle</location>
            <location evidence="6">Neuronal dense core vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NPY family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J4J5"/>
    <dbReference type="Ensembl" id="ENSEAST00005066834.1">
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        <property type="gene ID" value="ENSEASG00005025389.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156475"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098982">
        <property type="term" value="C:GABA-ergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098992">
        <property type="term" value="C:neuronal dense core vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005184">
        <property type="term" value="F:neuropeptide hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031841">
        <property type="term" value="F:neuropeptide Y receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021954">
        <property type="term" value="P:central nervous system neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021987">
        <property type="term" value="P:cerebral cortex development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007631">
        <property type="term" value="P:feeding behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007218">
        <property type="term" value="P:neuropeptide signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008217">
        <property type="term" value="P:regulation of blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099538">
        <property type="term" value="P:synaptic signaling via neuropeptide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00126">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001955">
        <property type="entry name" value="Pancreatic_hormone-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020392">
        <property type="entry name" value="Pancreatic_hormone-like_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10533">
        <property type="entry name" value="NEUROPEPTIDE Y/PANCREATIC HORMONE/PEPTIDE YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10533:SF5">
        <property type="entry name" value="PRO-NEUROPEPTIDE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00159">
        <property type="entry name" value="Hormone_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00278">
        <property type="entry name" value="PANCHORMONE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00309">
        <property type="entry name" value="PAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00265">
        <property type="entry name" value="PANCREATIC_HORMONE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50276">
        <property type="entry name" value="PANCREATIC_HORMONE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0027">Amidation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040234004" evidence="8">
        <location>
            <begin position="29"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005048064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="134" mass="14791" checksum="E3491A234A8284A0" modified="2023-09-13" version="1">MLGSKRLGLSGLTLALSLLVCLGALAEAYPSKPDNPGEDAPAEDLARYYSALRHYINLITRQRYGKRSSPETLISDLLMRESTENVPRTRYEKAPVGRCCGAQGSQGREIRGLPREEVLEWGLPDQVGFGQKWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J4X6</accession>
    <name>A0A9L0J4X6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="9">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">USP26</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14 15">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="9"/>
    <dbReference type="RefSeq" id="XP_044620067.1">
        <property type="nucleotide sequence ID" value="XM_044764132.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J4X6"/>
    <dbReference type="Ensembl" id="ENSEAST00005040666.1">
        <property type="protein sequence ID" value="ENSEASP00005058720.1"/>
        <property type="gene ID" value="ENSEASG00005025491.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005080225.1">
        <property type="protein sequence ID" value="ENSEASP00005044315.1"/>
        <property type="gene ID" value="ENSEASG00005025491.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106842126"/>
    <dbReference type="CTD" id="83844"/>
    <dbReference type="GeneTree" id="ENSGT00940000161929"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02257">
        <property type="entry name" value="Peptidase_C19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000124">
        <property type="entry name" value="ubiquitin carboxyl-terminal hydrolase 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.180:FF:000001">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000234">
        <property type="entry name" value="Ubiquitin specific peptidase 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.180">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 26/29/37, pleckstrin homology-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032069">
        <property type="entry name" value="USP37-like_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038093">
        <property type="entry name" value="USP37-like_PH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF711">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16674">
        <property type="entry name" value="UCH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="11">
        <location>
            <begin position="293"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="597"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="787"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="787"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="802"/>
            <end position="821"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005044315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="917" mass="104918" checksum="E2A258C2082F52CB" modified="2023-09-13" version="1">MAPLIVHGFAQIWSGNIRMSKTKEAYIRTVEEKKKVILVVSFNTGEYTTFQLVNIKNVVFRSYGENENQLNLIFQNDDFLFIEKLSLRDGKELKMFLESFRKNNQLPMRPDRVGGVFASRTIQKEINKTSFHKVCKKSSSGSYETGEGTGIPDLQKMPLFTSKLPTRTCEELLENRCEKGKRKLSSASETNEDIPKQSNSIRNKKSKTSLLRYVSHNEKKKLRLKESRENKKCGFSLKSNFTGNPYIDGTSLPRILYQKMYLAFLLEPKYSEDEPEWQKLKMSFEIYPEKVWEGLPNLGNTCYMNAVLQSLFSIPSFADDLLNQGFPWSKIPLDVLSMCLAQLLVLKDIYNIKIKEKLLMSIKKIISAVAKIFSGNIQNDAHEFLGHCLDQMKENIGRINTIWKARIESEEENSPQQVFASSAATEVLVCPVVTNFEFELLRSIICKACGRVVLRTEVHNYLSVNLPQGPKAVPSSIQSTFDLFFGVEELEYKCEKCEHRRSVAVHKFSRLPRVLIIHLKRYSFNEFCSLRKDDQEIIISKYLKLSSHCNESTKPPLPLNKNAHIRDLQILKIFQKIHSEAISSLTASTKLTLESKSSLTPHAGSGKESEPQKYQILNKGTSREQQQKDLGKYSKLNIIESKLLNSGHGTIIEKELLAASLMMGLEDTSFSLTHEGEGKSHSGPDTRVHPQEVLEHLKLKKYEKTRMFVDFESATETTKDFSEDKKARIPEGYPKVAKQIQQCKRMRIYEQALRQALLESLPEPDAQWHTEKLRRPTEVTLQEANVSSLGALGSNKNPGNKDVLDKEKTETKAKKPKRNAEIRDRHAYQLIGVVSHLGQTPDSGHYISDAYDFERQVWFTYNDLQVSSIQEALMQEARLCTGYIFFYMHNEIFEELLGREEKFRSSNAKAGETPQGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J524</accession>
    <name>A0A9L0J524_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Neuropilin and tolloid like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">NETO2</name>
    </gene>
    <organism evidence="16 17">
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
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                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="Liu"/>
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                <person name="Xu"/>
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                <person name="Liu"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
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        <property type="entry name" value="SUPPRESSOR OF CYTOKINE SIGNALING 3"/>
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    <sequence length="229" mass="25117" checksum="C36B3791DD159A6C" modified="2023-09-13" version="1">MVTHSKFPAAGMSRPLDTSLRLKTFSSKSEYQLVVNAVRKLQESGFYWSAVTGGEANLLLSAEPAGTFLIRDSSDQRHFFTLSVKTQSGTKNLRIQCEGGSFSLQSDPRSTQPVPRFDCVLKLVHHYMPPPGAPSLPSPPTEPSSSPSSEVPDQPPAQPLPGNPPRRAYYIYSGGEKIPLVLSRPLSSNVATLQHLCRKTVNGHLDSYEKVTQLPGPIREFLDQYDAPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
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    <name>A0A9L0J689_EQUAS</name>
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        <recommendedName>
            <fullName evidence="3 12">Eukaryotic translation initiation factor 2A</fullName>
            <shortName evidence="12">eIF-2A</shortName>
        </recommendedName>
    </protein>
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        <name evidence="15" type="primary">EIF2A</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 12">Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the WD repeat EIF2A family.</text>
    </comment>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:ribosome binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097193">
        <property type="term" value="P:intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001243">
        <property type="term" value="P:negative regulation of intrinsic apoptotic signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009967">
        <property type="term" value="P:positive regulation of signal transduction"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042255">
        <property type="term" value="P:ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="Eukaryotic translation initiation factor 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Eukaryotic translation initiation factor 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011387">
        <property type="entry name" value="TIF2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013979">
        <property type="entry name" value="TIF_beta_prop-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR13227">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR13227:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2A"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF08662">
        <property type="entry name" value="eIF2A"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
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    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 12" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 12" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="6" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Translation initiation factor beta propellor-like" evidence="14">
        <location>
            <begin position="216"/>
            <end position="411"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="433"/>
            <end position="534"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="494"/>
            <end position="507"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="517"/>
            <end position="529"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00003993"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009573"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013819"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR017222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005045527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="580" mass="64544" checksum="671EC032B9A455C1" modified="2023-09-13" version="1">MAPSTPLLTVRGSEGLYMVNGPPHFTESTVLPRESGKNCKVYTFSKDGTLFAWGDGEKINIINVTNKRLLHSFNLPKAVCLEFSPKNTVLATWQPYTTSKDGTAGIPNLQLYDVKTGTCLKSFNQKKMQNWCPSWSEDETICARNVNNEVHFFESNNFNTITNKLHLQKISDFVLSPGPQPYKVAVYVPGSKGAPSFVRLYQYPNFDGPHAALANKSFFKADKVTMLWNKKATAVLVIASTDVDKTGASYYGEQTLHYIATNGESAVVQLPKNGPIYDVVWNSSSAEFCAVYGFMPAKATIFNLKCDPVFDFGTGPRNAAYYSPHGHILVLAGFGNLRGQMEVWDVKNYKLISKPVASDSTYFAWCPDGEHILTATCAPRLRVNNGYKIWHYTGSVLHKYDVPSNGELWQVSWQPFLDGIFPAKTITYQAVPSDVPSEEPKVATAYRPPALRNKPITNSKLHEDEPPQNMKPQPGNDKPLSKTALKNQRKHEAKKAAKQEARSDKSPDLAPAPAPQSTPRNTLSHSTSGDPEIDRKIKNLRKKLKAIEQLKEQAATGKQLEKNQVFSAFFTWACSHYHPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JA30</accession>
    <name>A0A9L0JA30_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein BTG1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">B-cell translocation gene 1 protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">BTG1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Anti-proliferative protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BTG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070363151.1">
        <property type="nucleotide sequence ID" value="XM_070507050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JA30"/>
    <dbReference type="SMR" id="A0A9L0JA30"/>
    <dbReference type="Ensembl" id="ENSEAST00005061890.1">
        <property type="protein sequence ID" value="ENSEASP00005046080.1"/>
        <property type="gene ID" value="ENSEASG00005038206.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106831164"/>
    <dbReference type="CTD" id="694"/>
    <dbReference type="GeneTree" id="ENSGT00950000182952"/>
    <dbReference type="OMA" id="SHWFPDK"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.90:FF:000003">
        <property type="entry name" value="BTG1 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.90">
        <property type="entry name" value="Anti-proliferative protein, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002087">
        <property type="entry name" value="Anti_prolifrtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033332">
        <property type="entry name" value="BTG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036054">
        <property type="entry name" value="BTG-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22978">
        <property type="entry name" value="B-CELL TRANSLOCATION GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22978:SF30">
        <property type="entry name" value="PROTEIN BTG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07742">
        <property type="entry name" value="BTG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00310">
        <property type="entry name" value="ANTIPRLFBTG1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00099">
        <property type="entry name" value="btg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160696">
        <property type="entry name" value="BTG domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00960">
        <property type="entry name" value="BTG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01203">
        <property type="entry name" value="BTG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anti-proliferative protein" evidence="5">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="Anti-proliferative protein" evidence="6">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00033398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS01203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005046080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="171" mass="19209" checksum="EFED8BE09013BE60" modified="2023-09-13" version="1">MHPFYTRAATMIGEIAAAVSFISKFLRTKGLTSERQLQTFSQSLQELLAEHYKHHWFPEKPCKGSGYRCIRINHKMDPLIGQAAQRIGLSSQELFRLLPSELTLWVDPYEVSYRIGEDGSICVLYEASPAGGSTQNSTNVQMVDSRISCKEELLLGRTSPSKNYNMMTVSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JDA6</accession>
    <name>A0A9L0JDA6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Coiled-coil domain-containing protein 39</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CCDC39</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Required for assembly of dynein regulatory complex (DRC) and inner dynein arm (IDA) complexes, which are responsible for ciliary beat regulation, thereby playing a central role in motility in cilia and flagella. Probably acts together with CCDC40 to form a molecular ruler that determines the 96 nanometer (nm) repeat length and arrangements of components in cilia and flagella. Not required for outer dynein arm complexes assembly.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CCDC39 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JDA6"/>
    <dbReference type="Ensembl" id="ENSEAST00005039433.1">
        <property type="protein sequence ID" value="ENSEASP00005051169.1"/>
        <property type="gene ID" value="ENSEASG00005016830.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015010"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060285">
        <property type="term" value="P:cilium-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060287">
        <property type="term" value="P:epithelial cilium movement involved in determination of left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036159">
        <property type="term" value="P:inner dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033290">
        <property type="entry name" value="CCDC39"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18962">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18962:SF0">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24161">
        <property type="entry name" value="CCDC39"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="806"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="852"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="17"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="164"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="394"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="469"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="600"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="669"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="807"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="857"/>
            <end position="878"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005051169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="878" mass="102880" checksum="260F94C7A3E3202D" modified="2023-09-13" version="1">MSSEFLAELHWENGFAIPVANQENKMLEDQLSKLQDERSSLKDQLRDYEDRINAMTSHFKNVKQEFSFTQSLCKAREHEIESEEHFKIIAERELGRVKDEIQRLEDEMVSIREKKSDKENNIFKTTQKLDGLKRQMNWDQQALEAWLEESAHKDSDALTLQKYAQQDDNKIRALTLQLERLTLECNQRRKVLDNELTETLSVQLELDKAAQDFRKIHNERQELIKQWENTIEQMQKRDKDIDECALLDSLKATVNRSSSDLEALRKNISKVKKDILEETTRLHKIKNHNQIAKDKLKHITEKTMSIEEKATNLEDMLKEEERGVKEVEVQLNIVKDVLFKKVQELQAETTKEKAVVSEIEGTRSSLKHLNHQLHKLDFETLKQQEIMYSQDFYIQQVERRMSRLKGEINSEEKQALEAKVVELKKTFEDKKSTVGLLETQIKKLHNDLYFMKKSNSKNHDEKQLLMTKINELNLFNDRSEKELNEAKALKQDLMIEDNLLKLEVKRTREMLHSKAEEVLSLEKRKQQIRTAMEERTEEIKVHKTMLASQIRYVDQERQNISAEFHERLSKIDKLKNRYEILTVVMLPPEGEEEKTQAYYVIKAAQEKEELQREGDSLDAKINKAEKEIYALENTLQVLNSCNNNYKQSFKKVTPSSDEYELKIHLEEQKRAADEKYRYKQRQIRELQEDIQSMENTLEVIEHLTNNIKEKLSEKQAYSFQLRRETEEQKPKLERVTKQCAKLTKEIRRFKDTKDETLEEQDINLREVKQFHKIIDEMLVDVTEQNAEIRIILQTYFQQNGLELPAASTKGSRQSSRSPSQTSLLSARSSRSTSTSASQASIKVLELNFPTSTSLMGSSARPTSASSSSSNGKSKMSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JEL3</accession>
    <name>A0A9L0JEL3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Zinc finger and BTB domain-containing protein 7B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Krueppel-related zinc finger protein cKrox</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">T-helper-inducing POZ/Krueppel-like factor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Zinc finger protein Th-POK</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">ZBTB7B</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="17">Homodimerizes. Interacts with NCL, NEDD4 and YBX1. Interacts with HNRNPU (via RNA-binding RGG-box region); the interaction facilitates the recruitment of long non-coding RNA Blnc1 by ZBTB7B. Interacts with HDAC4 and HDAC5; the interaction allows the recruitment of HDAC4 and HDAC5 on CD8 loci for deacetylation and possible inhibition of CD8 genes expression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014692563.1">
        <property type="nucleotide sequence ID" value="XM_014837077.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044613710.1">
        <property type="nucleotide sequence ID" value="XM_044757775.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044613711.1">
        <property type="nucleotide sequence ID" value="XM_044757776.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070353777.1">
        <property type="nucleotide sequence ID" value="XM_070497676.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070353778.1">
        <property type="nucleotide sequence ID" value="XM_070497677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JEL3"/>
    <dbReference type="Ensembl" id="ENSEAST00005077788.1">
        <property type="protein sequence ID" value="ENSEASP00005050778.1"/>
        <property type="gene ID" value="ENSEASG00005019461.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106828512"/>
    <dbReference type="KEGG" id="eai:106828512"/>
    <dbReference type="CTD" id="51043"/>
    <dbReference type="GeneTree" id="ENSGT00940000160219"/>
    <dbReference type="OMA" id="SPEHHEL"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18327">
        <property type="entry name" value="BTB_POZ_ZBTB7B_ZBTB15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000115">
        <property type="entry name" value="Zinc finger and BTB domain containing 7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000572">
        <property type="entry name" value="Zinc finger and BTB domain containing 7C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000673">
        <property type="entry name" value="Zinc finger and BTB domain-containing 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000089">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000202">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF4">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING PROTEIN 7B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="24">
        <location>
            <begin position="34"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="25">
        <location>
            <begin position="348"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="25">
        <location>
            <begin position="376"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="25">
        <location>
            <begin position="404"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="25">
        <location>
            <begin position="432"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="171"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="458"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="23">
        <location>
            <begin position="182"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="23">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="23">
        <location>
            <begin position="510"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00062741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00070668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00078335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00081658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00083478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="541" mass="58245" checksum="D6C910AFA41EEB9C" modified="2023-09-13" version="1">MGSPEDDLIGIPFPEHSSELLSCLNEQRQLGHLCDLTIRTQGLEYRTHRAVLAACSHYFKKLFTEGGGGAVMGAGGGGTAAGGAGAGVCELDFVGPEALGALLEFAYTATLTTSSANMPAVLQAARLLEIPCVIAACMEILQVSGLEAPSPDEDDCERARQYLEAFATATASGVPNGEDSPPQVPLPPPPPPPPPPRPVARRSRKPRKAFLQTKGARANHLVPEVPTVPTHPLTYEEEEVAGKVGSSGGSGLVDSYSPPTGTASPPEGPLSYEPYEGEEEEEELVYPPAYGLAQSGGPPLSPEELGSDEDAIDPDLMAYLSSLHQDALAPGLDGQDKLVRKRRSQMPQECPVCHKIIHGAGKLPRHMRTHTGEKPFACEVCGVRFTRNDKLKIHMRKHTGERPYSCPHCPARFLHSYDLKNHMHLHTGDRPYECHLCHKAFAKEDHLQRHLKGQNCLEVRTRRRRKDDAPPHYPPPSAATPSPAGLDLSNGHLDTFRLSLARFWEQSAPTGPPVSTPGPPDDDEEEGAPTTPQAEGAMESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JER1</accession>
    <name>A0A9L0JER1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Placenta-expressed transcript 1 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PLET1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
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            <text>[G-protein-coupled receptor] + ATP = [G-protein-coupled receptor]-phosphate + ADP + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:11260"/>
            <dbReference type="Rhea" id="RHEA-COMP:11261"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.16"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0JW81"/>
    <dbReference type="Ensembl" id="ENSEAST00005047775.1">
        <property type="protein sequence ID" value="ENSEASP00005054297.1"/>
        <property type="gene ID" value="ENSEASG00005012304.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158544"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047696">
        <property type="term" value="F:beta-adrenergic receptor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004703">
        <property type="term" value="F:G protein-coupled receptor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05630">
        <property type="entry name" value="STKc_GRK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000009">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000074">
        <property type="entry name" value="G protein-coupled receptor kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000239">
        <property type="entry name" value="GPCR_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355:SF15">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24355">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE/RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00717">
        <property type="entry name" value="GPCRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RGS" evidence="13">
        <location>
            <begin position="59"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="186"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="14">
        <location>
            <begin position="449"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="311"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600239-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005054297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="649" mass="73365" checksum="0A658CDA29288DD8" modified="2023-09-13" version="1">MELENIVANTVLLKAREGGGGNRKGKSKKWRQMLQFPHISQCEELRHSLERDYHSLCERQPIGRLLFREFCATRPELTRCIAFLDGVAEYEVTPDEKRKACGQRLMQNFLSHTGPDLIPEVPRQLVTNCTQRLEQGPCKDLFQELTRLTHEYLSVAPFADYLDSIYFNRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGEAMALNEKQILEKVNSRFVVSLAYAYETKDALCLVLTLMNGGDLKFHIYHMGQAGFPEARAVFYAAEICCGLEDLHRERIVYRDLKPENILLDDHGHIRISDLGLAVHVPEGQTIKGRVGTVGYMAPEVVKNERYTFSPDWWALGCLLYEMIAGQSPFQQRKKKIKREEVERLVKEVQEEYSERFSPQARSLCSQLLCKDPAERLGCRGGSAREVKEHPLFKKLNFKRLGAGMLEPPFKPDPQAIYCKDVLDIEQFSTVKGVELEPTDQDFYQKFATGSVPIPWQNEMVETECFQELNVFGLDGSVPPDLDWKGQPPAPPKKGLLQRFFSRQVTPSSVPGPPAWLQGTVGGRQSWCPRALGVGLLQSCQWCPGSLASSPTLAGLTASFLFPEVSSVGSPWVGLTARPWGATGSPSMAEARCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JXN9</accession>
    <name>A0A9L0JXN9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Speckle targeted PIP5K1A-regulated poly(A) polymerase</fullName>
            <ecNumber evidence="6">2.7.7.19</ecNumber>
            <ecNumber evidence="7">2.7.7.52</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">RNA-binding motif protein 21</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">U6 snRNA-specific terminal uridylyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">TUT1</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>RNA(n) + UTP = RNA(n)-3'-uridine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14785"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17348"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173116"/>
            <dbReference type="EC" id="2.7.7.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="24">Associates with the cleavage and polyadenylation specificity factor (CPSF) complex. Interacts with CPSF1 and CPSF3; the interaction is direct. Interacts with PIP5K1A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DNA polymerase type-B-like family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="6"/>
    <dbReference type="EC" id="2.7.7.52" evidence="7"/>
    <dbReference type="RefSeq" id="XP_014689801.1">
        <property type="nucleotide sequence ID" value="XM_014834315.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JXN9"/>
    <dbReference type="Ensembl" id="ENSEAST00005040761.1">
        <property type="protein sequence ID" value="ENSEASP00005057914.1"/>
        <property type="gene ID" value="ENSEASG00005007531.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106826814"/>
    <dbReference type="KEGG" id="eai:106826814"/>
    <dbReference type="CTD" id="64852"/>
    <dbReference type="GeneTree" id="ENSGT00940000159914"/>
    <dbReference type="OrthoDB" id="2274644at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140767">
        <property type="term" value="F:enzyme-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="X."/>
                <person name="Guo"/>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="Q."/>
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                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <name evidence="25" type="primary">TRAF2</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 19">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TNF receptor-associated factor family. A subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0K3Y0"/>
    <dbReference type="Ensembl" id="ENSEAST00005048533.1">
        <property type="protein sequence ID" value="ENSEASP00005059513.1"/>
        <property type="gene ID" value="ENSEASG00005017474.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156621"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035631">
        <property type="term" value="C:CD40 receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990604">
        <property type="term" value="C:IRE1-TRAF2-ASK1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097057">
        <property type="term" value="C:TRAF2-GSTP1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002947">
        <property type="term" value="C:tumor necrosis factor receptor superfamily complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005174">
        <property type="term" value="F:CD40 receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046625">
        <property type="term" value="F:sphingolipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031996">
        <property type="term" value="F:thioesterase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043120">
        <property type="term" value="F:tumor necrosis factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007249">
        <property type="term" value="P:canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160162">
        <property type="term" value="P:CD27 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023035">
        <property type="term" value="P:CD40 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071732">
        <property type="term" value="P:cellular response to nitric oxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097400">
        <property type="term" value="P:interleukin-17-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048255">
        <property type="term" value="P:mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043124">
        <property type="term" value="P:negative regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038061">
        <property type="term" value="P:non-canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001238">
        <property type="term" value="P:positive regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032743">
        <property type="term" value="P:positive regulation of interleukin-2 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002726">
        <property type="term" value="P:positive regulation of T cell cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903265">
        <property type="term" value="P:positive regulation of tumor necrosis factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097300">
        <property type="term" value="P:programmed necrotic cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070534">
        <property type="term" value="P:protein K63-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002637">
        <property type="term" value="P:regulation of immunoglobulin production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046328">
        <property type="term" value="P:regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043254">
        <property type="term" value="P:regulation of protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023019">
        <property type="term" value="P:signal transduction involved in regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042110">
        <property type="term" value="P:T cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905669">
        <property type="term" value="P:TORC1 complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905670">
        <property type="term" value="P:TORC2 complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033209">
        <property type="term" value="P:tumor necrosis factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03778">
        <property type="entry name" value="MATH_TRAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16639">
        <property type="entry name" value="RING-HC_TRAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000035">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000189">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000263">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000291">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000035">
        <property type="entry name" value="TNF receptor-associated factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012227">
        <property type="entry name" value="TNF_rcpt-assoc_TRAF_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049342">
        <property type="entry name" value="TRAF1-6_MATH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037305">
        <property type="entry name" value="TRAF2_MATH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027133">
        <property type="entry name" value="TRAF2_RING-HC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049441">
        <property type="entry name" value="TRAF2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032070">
        <property type="entry name" value="TRAF_BIRC3-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF21">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21355">
        <property type="entry name" value="TRAF-mep_MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21341">
        <property type="entry name" value="TRAF2_zf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16673">
        <property type="entry name" value="TRAF_BIRC3_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02176">
        <property type="entry name" value="zf-TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015614">
        <property type="entry name" value="TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57953">
        <property type="entry name" value="Trimerization domain of TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="18" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 19 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="13" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="14 19 20" id="KW-0862">Zinc</keyword>
    <keyword evidence="12 20" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="85"/>
            <end position="123"/>
        </location>
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            <begin position="176"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="TRAF-type" evidence="24">
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            <begin position="228"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="23">
        <location>
            <begin position="417"/>
            <end position="562"/>
        </location>
    </feature>
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            <begin position="176"/>
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    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="20">
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            <begin position="228"/>
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        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="331"/>
            <end position="393"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006608"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="ARBA" id="ARBA00022703"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022786"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022990"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR015614"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005059513.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="567" mass="63229" checksum="C046E6B5E7F1FB38" modified="2023-09-13" version="1">MGPREMCFQGCAGNKALECPRRRLGRSSWVSSFIMSWLGSRAWQFTGVAALMAAASVTPPGSLDLLQPGFSKTLLGTKLEDKYLCSACKNVLRRPFQAQCGHRYCSYCLTSILSSGPQNCAACVHEGIYEEGISILESSSAFPDNAARREVESLPAVCPSDGCTWKGTLKEYESCHEGHCPFMLTECPACKGLVRLGEKEHHLEHECPERSLSCRHCRAPCCWADMKAHHDVCPKFPLTCDGCGKKKISREKFQDHVRTCGKCRVPCRFHAVGCPEMVESEKQQEHEAKWLQEHLAMLLGVLLDTKHLLGDSSSFLGQSQVGNPGAEVSKAAELLQRCEALERKTATFENIVCVLNREVERVAMTAEACGRQHRLDQDRIEALSNKVQQLERSIGLKDLAMADLEQKVHEMEASTFDGVFIWKISDFTRKRQEAVAGRTPAIFSPAFYTSRYGYKMCLRIYLNGDGTGRGTHVSLFFVVMKGPNDALLRWPFNQKVTLMLLDQNNREHVIDAFRPDVTSSSFQRPVSDMNIASGCPLFCPVSKMEAKNSYVRDDAIFIKAIVDLTGL</sequence>
</entry>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
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        <name evidence="3" type="primary">SFT2D3</name>
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    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K5Y4"/>
    <dbReference type="Ensembl" id="ENSEAST00005061687.1">
        <property type="protein sequence ID" value="ENSEASP00005060754.1"/>
        <property type="gene ID" value="ENSEASG00005038488.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="158"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
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            <begin position="237"/>
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            <begin position="1"/>
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            <begin position="241"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="320"/>
            <end position="331"/>
        </location>
    </feature>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005060754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="449" mass="44944" checksum="C51B07FED10A846B" modified="2023-09-13" version="1">MATARVAGAVRADAGAPSPRGSLWHPFTAGRRAGTNPGRTKTTPRETQLRARRPAQRAAPPLPPPGPSGTGSSAPPPPRGHPVQDGGPAPPAAGVPGTGESRRAGSRGAGRGARGGERAGGGMAGPRGPALDVGAEPAEPSAAAGPACLPSVTRAQRAGGASGVCLLLAALCFGLAALYAPALLLRARKFALLLVWARRWRWRAGALLRGGAACGRLPARRGGAVAARAALRGRAGRHAVRGAGPAQHGAHGAGRMRAGGRPVGAAPRPAALGHGLRAASRPRAAGLGRPPRQGAARVRGPGTASRQGRGAGRRARTRNRPGERTRSRPSGEDAVPTPGRGSGAGASCARAGAQAVGLERLSGSPAVARPEAAPGIGWTCRDADDELLLRSPRARTELSGNSGSAALTGRPVPLPDSSPVTAVAECSSLATVSRCLLRLLESIFPHSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K600</accession>
    <name>A0A9L0K600_EQUAS</name>
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        <recommendedName>
            <fullName evidence="16">Integrin-linked kinase-associated serine/threonine phosphatase 2C</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ILKAP</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Zhao"/>
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                <person name="Wang"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
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                <person name="Hou"/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
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                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Protein phosphatase that may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. Selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. Inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the PP2C family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0K600"/>
    <dbReference type="Ensembl" id="ENSEAST00005071877.1">
        <property type="protein sequence ID" value="ENSEASP00005060896.1"/>
        <property type="gene ID" value="ENSEASG00005007403.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157403"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 17" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
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            <begin position="23"/>
            <end position="470"/>
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            <begin position="186"/>
            <end position="468"/>
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            <begin position="105"/>
            <end position="170"/>
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            <begin position="134"/>
            <end position="148"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
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            <dbReference type="ARBA" id="ARBA00004496"/>
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            <dbReference type="ARBA" id="ARBA00006702"/>
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            <dbReference type="ARBA" id="ARBA00013081"/>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00072449"/>
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            <dbReference type="RuleBase" id="RU003465"/>
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            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="470" mass="52229" checksum="BCA94322923A4150" modified="2023-09-13" version="1">MPTLCQAWFYLLLIKVSSQAYAAMRETENKYMIWQMVFTGQEEKQVRSRGQSNVVEAGCRFRLWAPHLLEQYGGCHIRHSERELQWHQGSVPGVCHGKEAQRGPLLFDDLPPASSTDSGSGGPLLFDDLPPASSGDSGSLDTSISQMVKNEGKGAKRKTSEEEKNGSEELVEKKVCKASSAIFALKGYVAERKGEREEMQDAHVILNDITEECKPPSSLITRVSYFAVFDGHGGIRASKFAAQNLHQNLIRKFPKGDVISVEKTVKRCLLDTFKHTDEEFLKQASSQKPAWKDGSTATCVLAVDNILYIANLGDSRAILCRYNEESQKHAALSLSKEHNPTQYEERMRIQKAGGNVRDGRVLGVLEVSRSIGDGQYKRCGVTSVPDIRRCQLTPNDRFILLACDGLFKVFTPEEAVNFILSCLEDEKIQSREGKPAVDARYEAACNRLANKAVQRGSADNVTVMVVRIGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KB30</accession>
    <name>A0A9L0KB30_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ubiquitin-ribosomal protein eS31 fusion protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RPS27A</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the 40S subunit of the ribosome. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Part of the 40S ribosomal subunit. Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">In the N-terminal section; belongs to the ubiquitin family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014843008.3"/>
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    <dbReference type="SMR" id="A0A9L0KB30"/>
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        <property type="protein sequence ID" value="ENSEASP00005058480.1"/>
        <property type="gene ID" value="ENSEASG00005038395.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106832295"/>
    <dbReference type="KEGG" id="eai:106832295"/>
    <dbReference type="CTD" id="6233"/>
    <dbReference type="GeneTree" id="ENSGT00910000144152"/>
    <dbReference type="OMA" id="GVFMAFH"/>
    <dbReference type="OrthoDB" id="428577at2759"/>
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        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01803">
        <property type="entry name" value="Ubl_ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000008">
        <property type="entry name" value="Ubiquitin-40S ribosomal protein S27a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_eS31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038582">
        <property type="entry name" value="Ribosomal_eS31_euk-type_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
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    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
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    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
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    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="UBQ"/>
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    <dbReference type="SUPFAM" id="SSF54236">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="13">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005058480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="156" mass="17965" checksum="617BC63DF3A904F7" modified="2023-09-13" version="1">MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGAKKRKKKSYTTPKKNKHKRKKVKLAVLKYYKVDENGKISRLRRECPSDECGAGVFMASHFDRHYCGKCCLTYCFNKPEDK</sequence>
</entry>
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    <accession>A0A9L0KBH5</accession>
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        <recommendedName>
            <fullName evidence="8">PDZ and LIM domain protein 7</fullName>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Wang"/>
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                <person name="Y."/>
                <person name="Liu"/>
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                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
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                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KBH5"/>
    <dbReference type="Ensembl" id="ENSEAST00005045074.1">
        <property type="protein sequence ID" value="ENSEASP00005058610.1"/>
        <property type="gene ID" value="ENSEASG00005012418.2"/>
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    <dbReference type="GeneID" id="106827152"/>
    <dbReference type="KEGG" id="eai:106827152"/>
    <dbReference type="CTD" id="9260"/>
    <dbReference type="GeneTree" id="ENSGT00940000159626"/>
    <dbReference type="OrthoDB" id="5911912at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:filamentous actin"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:nucleoplasm"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="C:Z disc"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:actin binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061061">
        <property type="term" value="P:muscle structure development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09452">
        <property type="entry name" value="LIM1_Enigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09456">
        <property type="entry name" value="LIM2_Enigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09458">
        <property type="entry name" value="LIM3_Enigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06753">
        <property type="entry name" value="PDZ_PDLIM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000019">
        <property type="entry name" value="LIM domain binding 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000010">
        <property type="entry name" value="PDZ and LIM domain protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000014">
        <property type="entry name" value="PDZ and LIM domain protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000020">
        <property type="entry name" value="PDZ and LIM domain protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050604">
        <property type="entry name" value="PDZ-LIM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF0">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 9" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDZ" evidence="12">
        <location>
            <begin position="10"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="11">
        <location>
            <begin position="281"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="11">
        <location>
            <begin position="340"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="11">
        <location>
            <begin position="400"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="81"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="176"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00039368"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005058610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="458" mass="49885" checksum="09C3947DA08F0D2D" modified="2023-09-13" version="1">MDSFKVVLDGPAPWGFRLQGGKDFNVPLSISRLTPGGKAAQAGVAVGDWVLSIDGENAGGLTHIEAQNKIRACGERLSLGLSRAQPAQSKPQKALAPAADPPRYTFAPSASLNKTARPFGAPLLADSAPQQNGQPLRPLVPDASKQRLMEDTEDWRPRPGTGQSRSFRILAHLTGTEFMQDPDEEHLKKSSQVPRTEAPTPASATPQEPWPGPTTPSPTSRPPWAVDPAFAERYAPDKTSTVLTRHSQPATPTPLQNRTSIVQAAAGGGTGGGSGNNGKTPVCHQCHKVIRGRYLVVLGHAYHPEEFVCSQCGKVLEEGGFFEEKGAIFCPPCYDVRYAPSCAKCKKKITGEIMHALKMTWHVHCFTCAACKTPIRNRAFYMEEGAPYCERDYEKMFGTKCRGCDFKIDAGDRFLEALGFSWHDTCFVCAICQINLEGKTFYSKKDKPLCKSHAFSHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KBL8</accession>
    <name>A0A9L0KBL8_EQUAS</name>
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        <recommendedName>
            <fullName evidence="13">Pro-cathepsin H</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">CTSH</name>
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    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Fang"/>
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                <person name="Zhao"/>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
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                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="Liu"/>
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                <person name="M."/>
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                <person name="X. and Zhong"/>
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Important for the overall degradation of proteins in lysosomes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-|-Xaa bonds) as well as an endopeptidase.</text>
            <dbReference type="EC" id="3.4.22.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044619664.2">
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                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014704089.2">
        <property type="nucleotide sequence ID" value="XM_014848603.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0KHP9"/>
    <dbReference type="Ensembl" id="ENSEAST00005043929.1">
        <property type="protein sequence ID" value="ENSEASP00005061065.1"/>
        <property type="gene ID" value="ENSEASG00005036312.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106835976"/>
    <dbReference type="KEGG" id="eai:106835976"/>
    <dbReference type="GeneTree" id="ENSGT01010000222320"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15405">
        <property type="entry name" value="7tmA_OR8B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000004">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
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    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="27"/>
            <end position="48"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="91"/>
            <end position="109"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="130"/>
            <end position="151"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="200"/>
            <end position="225"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
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    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="310" mass="34695" checksum="C8855943CE0E02F7" modified="2023-09-13" version="1">MARGNGSFITQFILVGLTDQPDLKLPLFFLFLVMYIVTVLGNMGLITIIGLNSHLHTPMYFFLFNLSFIDLSYSSTFTPKMLVNFIFKQNIISYMGCMTQLYFFCFFSISECYVLTSMAYDRYVAIRKPLLYSVVMSPKVCSSLMLGSYFMAFSGATAHTGCMLRLTFCDANTINHYLCDIFPLLQLSCTSTYVNELEVFIVGGINVIVPSVAIFVSYGFILSSILHISSTEGRSKAFGTCSSHIIAVSLFFGSAAFMYLKPSSAVSMGEGKISSVFYTNTVPLMNPLIYSLRNKDVKIALRKTLSMRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="11">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="12" type="primary">LOC106829233</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A8C4KRK9"/>
    <dbReference type="Ensembl" id="ENSEAST00005000518.2">
        <property type="protein sequence ID" value="ENSEASP00005000427.2"/>
        <property type="gene ID" value="ENSEASG00005000407.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00990000203527"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:cellular response to fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0042593">
        <property type="term" value="P:glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002385">
        <property type="term" value="P:mucosal immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045776">
        <property type="term" value="P:negative regulation of blood pressure"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0046676">
        <property type="term" value="P:negative regulation of insulin secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019228">
        <property type="term" value="P:neuronal action potential"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002879">
        <property type="term" value="P:positive regulation of acute inflammatory response to non-antigenic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032722">
        <property type="term" value="P:positive regulation of chemokine production"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002720">
        <property type="term" value="P:positive regulation of cytokine production involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904457">
        <property type="term" value="P:positive regulation of neuronal action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003062">
        <property type="term" value="P:regulation of heart rate by chemical signal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046885">
        <property type="term" value="P:regulation of hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046626">
        <property type="term" value="P:regulation of insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd15170">
        <property type="entry name" value="7tmA_FFAR2_FFAR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000173">
        <property type="entry name" value="Free fatty acid receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013312">
        <property type="entry name" value="GPR40-rel_orph"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45822">
        <property type="entry name" value="FREE FATTY ACID RECEPTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45822:SF6">
        <property type="entry name" value="FREE FATTY ACID RECEPTOR 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01904">
        <property type="entry name" value="GPR40FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="54"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="134"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="190"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="263"/>
            <end position="281"/>
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    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="33"/>
            <end position="278"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023224"/>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000427.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="290" mass="32401" checksum="6C60722464C82B46" modified="2023-09-13" version="2">MTMDTSPDQSFSPGNHWLFFSVYLFSFLVGLPLNLVALVIFVGKLRRRPVAVDVLLLNLTLSDLLLLLFLPFRMAEAASDMHWPLPFILCPFSGSLFFTTIYLTSLFLAAVSIERFLSVAYPLWYKTRPRLGQAGLVSGACWLLAGAHCSVVYIIEFSGNSSMGQGKNGTCYLEFREDQLAILLPVRLEMAVVLFGVPLLITSYCYGHLVWLLGRGASHHRRRRVAGLVAATLLNFLVCFAPYNMSHVVGYVQGKSPTWRSYVLLLSTLNSCVDPLVYYFSSSGFQADFH</sequence>
</entry>
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            <fullName evidence="15">2',5'-phosphodiesterase 12</fullName>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Eutheria</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <reaction evidence="1">
            <text>Exonucleolytic cleavage of poly(A) to 5'-AMP.</text>
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        </reaction>
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    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CCR4/nocturin family.</text>
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    <dbReference type="EC" id="3.1.13.4" evidence="5"/>
    <dbReference type="RefSeq" id="XP_014724211.1">
        <property type="nucleotide sequence ID" value="XM_014868725.3"/>
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        <property type="protein sequence ID" value="ENSEASP00005000723.2"/>
        <property type="gene ID" value="ENSEASG00005000644.2"/>
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        <property type="component" value="Chromosome 21"/>
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        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004535">
        <property type="term" value="F:poly(A)-specific ribonuclease activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0140374">
        <property type="term" value="P:antiviral innate immune response"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0071359">
        <property type="term" value="P:cellular response to dsRNA"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0035457">
        <property type="term" value="P:cellular response to interferon-alpha"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0071346">
        <property type="term" value="P:cellular response to type II interferon"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000958">
        <property type="term" value="P:mitochondrial mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045070">
        <property type="term" value="P:positive regulation of viral genome replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044528">
        <property type="term" value="P:regulation of mitochondrial mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="2',5'-phosphodiesterase 12"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050410">
        <property type="entry name" value="CCR4/nocturin_mRNA_transcr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005135">
        <property type="entry name" value="Endo/exonuclease/phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048821">
        <property type="entry name" value="PDE12-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121:SF37">
        <property type="entry name" value="2',5'-PHOSPHODIESTERASE 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12121">
        <property type="entry name" value="CARBON CATABOLITE REPRESSOR PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03372">
        <property type="entry name" value="Exo_endo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21171">
        <property type="entry name" value="PDE12-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="2',5'-phosphodiesterase 12-like N-terminal" evidence="19">
        <location>
            <begin position="161"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Endonuclease/exonuclease/phosphatase" evidence="18">
        <location>
            <begin position="299"/>
            <end position="599"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="89"/>
            <end position="115"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="203"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="203"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00083541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF03372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF21171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000723.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="609" mass="67160" checksum="75110287662AC422" modified="2023-09-13" version="2">MWRLPGARAALRGVRTAVERHSRAEAATETGTGAMERAVVRCVPSEPKLSLSFALADGSHKNMQRDQSEPLGRVLSRIATNALKGHAKAAAAKKSRKNRPNASGGVACAGPGSEPAAACEPVVKLYYREEAVAEDVLNVDAWQDGAVLQIGDVKYKVERNPPAFTELQLPRYIMAGFPVCPKLSLEFGDPASSLFRWYKEAKPGAAEPEGGGPSSLSPSSSSPGWTETGVDERVYTPSNADIGLRLKLHCTPGNGQRFGPSRELESVCLVEAGPGTCTFDHRHLYTKKVTDDALIRTVSYNILADTYAQTEFSRTVLYPYCAPYALELDYRQNLIQKELTGYNADLICLQEVDRSVFTDSLVPALEAFGLEGVFRIKQHEGLATFYRKSKFSLLSQHDISFHEALESDPLHKELLEKLVLYPSAQERVLQRSSVLQVSVLQSRQDSSKKICVANTHLYWHPKGGYIRLIQMAVALAHIRHVSCDLYPGIPVIFCGDFNSTPSTGMYHFVINGNIPEDHEDWASNGEEERCNMSLAHFFKLKSACGEPAYTNYVGGFHGCLDYIFIDLNALEVEQVIPLPSHEEVTTHQALPSVSHPSDHIALVCDLKWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KT23</accession>
    <name>A0A8C4KT23_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Lectin, mannose binding 2 like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LMAN2L</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
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                <person name="M."/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Endomembrane system</location>
            <topology evidence="11">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="ENSEASG00005001367.2"/>
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        <property type="component" value="Chromosome 6"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="entry name" value="Vesicular integral-membrane protein VIP36"/>
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        <property type="entry name" value="ConA-like_dom_sf"/>
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        <property type="entry name" value="Intracellular_Lectin-GPT"/>
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        <property type="entry name" value="Lectin_leg"/>
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        <property type="entry name" value="VESICULAR MANNOSE-BINDING LECTIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="VIP36-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF03388">
        <property type="entry name" value="Lectin_leg-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
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        <property type="entry name" value="L_LECTIN_LIKE"/>
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    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5" id="KW-0430">Lectin</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="340"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="L-type lectin-like" evidence="13">
        <location>
            <begin position="66"/>
            <end position="302"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004194"/>
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    </evidence>
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    <sequence length="376" mass="43078" checksum="194320E6F1307874" modified="2023-09-13" version="2">MAWTTRRVFSEGTAVGKMAASLGSSGWWQRWRRRLSARDGSRMLLLLLLLGSGQGPRQVGAGQTFEYLKREHSLSKPYQGVGTSSSSLWNLMGNAMVMTQYIRLTPDMQSKQGALWNRVPCFLRDWELQVHFKIHGQGKKNLHGDGLAIWYTKDRMQPGPVFGNMDKFVGLGVFVDTYPNEEKQQEAQKRRYSPGVQRVFPYISAMVNNGSLSYDHERDGRPTELGGCTAIVRNLHYDTFLVIRYVKRHLTIMMDIDGKHEWRDCIEVPGVRLPRGYYFGTSSITGDLSDNHDVISLKLFELTVERTPEEERLHRDVFLPSVDNMKLPEMTAPLPPLSGLALFLIVFFSLVFSVFAIVIGIILYNKWQEQSRKRFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KV41</accession>
    <name>A0A8C4KV41_EQUAS</name>
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            <fullName evidence="12">Nuclear factor of activated T-cells, cytoplasmic 1</fullName>
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            <fullName evidence="13">NFAT transcription complex cytosolic component</fullName>
        </alternativeName>
    </protein>
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        <name evidence="16" type="primary">NFATC1</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells. Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. NFATC proteins bind to DNA as monomers. Interacts with HOMER2 and HOMER3; this interaction may compete with calcineurin/PPP3CA-binding and hence prevent NFATC1 dephosphorylation and activation. Interacts with TLE6/GRG6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044599946.1">
        <property type="nucleotide sequence ID" value="XM_044744011.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KV41"/>
    <dbReference type="Ensembl" id="ENSEAST00005002871.2">
        <property type="protein sequence ID" value="ENSEASP00005002611.2"/>
        <property type="gene ID" value="ENSEASG00005001978.2"/>
    </dbReference>
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    <dbReference type="CTD" id="4772"/>
    <dbReference type="GeneTree" id="ENSGT00940000157616"/>
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        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033173">
        <property type="term" value="P:calcineurin-NFAT signaling cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07881">
        <property type="entry name" value="RHD-n_NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000040">
        <property type="entry name" value="Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.340:FF:000001">
        <property type="entry name" value="Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.340">
        <property type="entry name" value="Rel homology domain (RHD), DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002909">
        <property type="entry name" value="IPT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008366">
        <property type="entry name" value="NFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032397">
        <property type="entry name" value="RHD_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011539">
        <property type="entry name" value="RHD_DNA_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037059">
        <property type="entry name" value="RHD_DNA_bind_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR12533">
        <property type="entry name" value="NFAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12533:SF5">
        <property type="entry name" value="NUCLEAR FACTOR OF ACTIVATED T-CELLS, CYTOPLASMIC 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16179">
        <property type="entry name" value="RHD_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00554">
        <property type="entry name" value="RHD_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01789">
        <property type="entry name" value="NUCFACTORATC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00429">
        <property type="entry name" value="IPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50254">
        <property type="entry name" value="REL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RHD" evidence="15">
        <location>
            <begin position="410"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="199"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="754"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="804"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="203"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="231"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="822"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="844"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="854"/>
            <end position="868"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00064204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005002611.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="943" mass="101374" checksum="0A35398AD8B77767" modified="2023-09-13" version="2">MPSTSFPGPSKFPLGPPAAVCGSGETLRPAPPAGGNMKSAEEEHYSYASSSIGSGLPLSTAHPSLPAPCHDLQTVAPGISAVASASHPSGYGGAVDSGPSGYFLSSSNVRPNGAPALESPRIEITSYLGLHHNNNQFFHDVEVEDVVPNSKRSPSTATLNLPNLEAYRDPSCLSPASSLSSRSCNSEASSYESNYSYPYASPQTSPWQSPCVSPKTTDPEEGYPRGLGACSLLSSPRHSPSTSPRASVTEESWLGARTSRPSSPCNKRKYGLNGRQLSCSPHHSPTPSPHSSPRVSVTDDTWLGNSTQYTSSAIVAAINALSTDSSLDLGDGVPVKTRKTALDHSPSVALKVEPAGEDLGTTPPTSDFLPEEFSSFQHIRKGAFCDQYLSVPQHPYQWARPRSPTSYTSPSLPALDWQLPSHSGPYELRIEVQPKSHHRAHYETEGSRGAVKASAGGHPSVQLHGYLESEPLTLQLFIGTADDRLLRPHAFYQVHRITGKTVSTTSHEVILSNTKVLEIPLLPENNMRAIIDCAGILKLRNSDIELRKGETDIGRKNTRVRLVFRVHIPQPNGRTLSLQVASNPIECSQRSAQELPLVEKQSLSSCPVLGGKKMVLSGHNFLQDSKVVFVEKAPDGHHIWEMEAKTDRDLCKPNSLVVEIPPFRNQRITSPVQVNFYVCNGKRKRSQYQHFTYLPANVPIIKTEPTDDYEPALTCGPMSQGLSPLPKPCYSQQLAMPPDPGSCLVAGFPPCPQRGSMMSPPPSASPKLHDLSSAPYSKGMASPGHSHLGLQRPAGGVLAVQEAPRPVGVHPGSPQQPPPALLQPQVSPQLSSSCSPGRQPALCPNSSSSPLPSGTQEPTCLQSCSPTRPSVMGHQQHPPPKVPRNESAATQPLPLPEVREDSNHSLAPIPVTVKREPQELDQLYLDDVNEIIRNDLSSTNVHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KVR2</accession>
    <name>A0A8C4KVR2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Rho GTPase-activating protein 33</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Rho-type GTPase-activating protein 33</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Sorting nexin-26</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Tc10/CDC42 GTPase-activating protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ARHGAP33</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">May be involved in several stages of intracellular trafficking. Could play an important role in the regulation of glucose transport by insulin. May act as a downstream effector of RHOQ/TC10 in the regulation of insulin-stimulated glucose transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Specifically interacts with CDC42 and RHOQ/TC10 through its Rho-GAP domain. Interacts with NEK6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the PX domain-containing GAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KVR2"/>
    <dbReference type="Ensembl" id="ENSEAST00005001868.2">
        <property type="protein sequence ID" value="ENSEASP00005001675.2"/>
        <property type="gene ID" value="ENSEASG00005001352.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155110"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150052">
        <property type="term" value="P:regulation of postsynapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04384">
        <property type="entry name" value="RhoGAP_CdGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11835">
        <property type="entry name" value="SH3_ARHGAP32_33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000002">
        <property type="entry name" value="rho GTPase-activating protein 32 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000030">
        <property type="entry name" value="rho GTPase-activating protein 32 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1520.10:FF:000095">
        <property type="entry name" value="Rho GTPase-activating protein 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051576">
        <property type="entry name" value="PX-Rho_GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15729">
        <property type="entry name" value="CDC42 GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15729:SF11">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 14" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="204"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="17">
        <location>
            <begin position="333"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="569"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="829"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="879"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1108"/>
            <end position="1280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="590"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="640"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="694"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="769"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="918"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="962"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1002"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1168"/>
            <end position="1182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="1267"/>
            <end position="1280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008795"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00053764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00065948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001675.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1280" mass="136855" checksum="66EC46D27C2DCC91" modified="2023-09-13" version="2">MGWRGTDPRTRPKEKVGVTGARSTDSLDGPGEGSVQPLPPTGGPSVKGKPGKRLSAPRGPFPRLADCAHFHYENVDFGHIQLLLSPEREGPNFSGENELVFGVQVTCQGRSWPVLRSYDDFRSLDAHLHRCIFDRRFSCLPELPPPPEGARAAQMLVPLLLQYLETLSGLVDSNLNCGPVLTWMELDNHGRRLLLSEEASLNIPAVAAAHVVKRYTAQAPDELSFEVGDIVSVIDMPPTEDRSWWRGKRGFQVGFFPSECVELFTERPGPGLKGDADGPPCGVPAPQGISSLTSAVPRPRGKLAGLLRTFMRSRPSRQRLRQRGILRQRVFGCDLGEHLSNSGQDVPQVLRCCSEFIEAHGVVDGIYRLSGVSSNIQRLRHEFDSERIPELSGPAFLQDIHSVSSLCKLYFRELPNPLLTYQLYGKFSEAMSVPGEEERLVRVHDVIQQLPPPHYRTLEYLLRHLARMARHSANTSMHARNLAIVWAPNLLRSMELESVGLGGAAAFREVRVQSVVVEFLLTHVDVLFSDTFTSAGLDPAGRCLLPRPKSLAGSGPSTRLLTLEEAQARTQGRLGTPTEVTAPKTPASPVERRKRERGEKQRKPGGSSWKTFFALGRGPSIPRKKPLPWLGGTRAPPQPSGSRPDTVTLRSAKSEESLSSQASGAGLQRLHRLRRPHSSTDAFPVGPAPAGSCESLSSSSSSSSSSSSSSSSSGSSAAGLEALSGSPSHRTSAWLDDGDELDFSPPHCLEGLRGLDFDPLTFRCSSPTPGDPAPPASPAPPAPASAFPPRAPPQALSPRGPASPASPTALDISEPLAVSVPPAVLELLGAGGTPASVTPTPALSPSPGLRPHLIPLLLRGAEAQLSDTCQQEICSKLALPGPRGAQGQHGPGVDSPLLPPPLSLLRPGGAPPPPPKNAARLMALALAERAQQVAQRQSQQEHGSTPAAPCSPFRRSLSLEVGGEPPGTSGTGPTPHSLAHPRAWAPGPPPSLPRQPTDGSLVRSQRPTGTSRRGLRGPAQVPTPSCFSSAPRECLPPFLGVPKPGLYPLGSPPFQPCSPAPAWRSPLGGPAPLDRGENLYYEIETGEGSYPGPTRSWSPFRSMPPDRLNASYGMLGQSPPPHRSPDFLLGYPPPPSCFPHDHLGYSAPQHPARRPTRPEPLYVNLALGPRGPSPASSSSSSPPAHPRSRSDPGPPAPRLPQKQRAPWGPHTAHRVPGPWGPPEPLLLYRAAPPAYGRGGEHHRGSLYRNGGQGREGAGPPPPYPTPSWSLHSEGQTRSYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KZ26</accession>
    <name>A0A8C4KZ26_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070344688.1">
        <property type="nucleotide sequence ID" value="XM_070488587.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KZ26"/>
    <dbReference type="Ensembl" id="ENSEAST00005001355.2">
        <property type="protein sequence ID" value="ENSEASP00005001197.2"/>
        <property type="gene ID" value="ENSEASG00005026061.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106823620"/>
    <dbReference type="GeneTree" id="ENSGT00950000182718"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15413">
        <property type="entry name" value="7tmA_OR8K-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="92"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="41"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001197.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="318" mass="36308" checksum="058C7A5C21BCEBAB" modified="2023-09-13" version="2">MEKHNQTMLNEFILVGITDNPELQAPLFGLFLIIYVISVVGNLGMIILTKTDSRLQTPMYFFLRHLAFTDLGYSTTVGPKMLVNFVVDHNTISYYFCATQLAFFSMFILSELFILSAMSYDRYVAICSPLLYTVIMSQRVCRLLVAIPYLYSTFVSLLVTIKIFNLSFCGYNVIHHFYCDSLPLLSLLCSNTSEIEMIILILAGFDLISFLIVLVSYLLILRAVLRMNSAEGRRKAFSTCGSHLTVVTVFYGTLIFIYTQPKSTHFFDTDKVASIFYTLIIPMLNPLIYSLRNTDVKYAVNKLWKSYAISFLKVHCSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L195</accession>
    <name>A0A8C4L195_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Alpha-galactosidase</fullName>
            <ecNumber evidence="15">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">NAGA</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Required for the breakdown of glycolipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Cleavage of non-reducing alpha-(1-&gt;3)-N-acetylgalactosamine residues from human blood group A and AB mucin glycoproteins, Forssman hapten and blood group A lacto series glycolipids.</text>
            <dbReference type="EC" id="3.2.1.49"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a globoside IV3GalNAc-Gb4Cer + H2O = N-acetyl-alpha-D-galactosamine + a globoside Gb4Cer</text>
            <dbReference type="Rhea" id="RHEA:48412"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:40356"/>
            <dbReference type="ChEBI" id="CHEBI:88167"/>
            <dbReference type="ChEBI" id="CHEBI:90400"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:48413"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a neolactoside IV(3)-alpha-GalNAc,IV(2)-alpha-Fuc-nLc4Cer(d18:0) + H2O = a neolactoside IV(2)-alpha-Fuc-nLc4Cer(d18:0) + N-acetyl-alpha-D-galactosamine</text>
            <dbReference type="Rhea" id="RHEA:49304"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:40356"/>
            <dbReference type="ChEBI" id="CHEBI:91118"/>
            <dbReference type="ChEBI" id="CHEBI:91119"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:49305"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a neolactoside IV(3)-alpha-GalNAc,IV(2)-alpha-Fuc-nLc4Cer(d18:1(4E)) + H2O = a neolactoside IV(2)-alpha-Fuc-nLc4Cer(d18:1(4E)) + N-acetyl-alpha-D-galactosamine</text>
            <dbReference type="Rhea" id="RHEA:48212"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28471"/>
            <dbReference type="ChEBI" id="CHEBI:28691"/>
            <dbReference type="ChEBI" id="CHEBI:40356"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:48213"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 15">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the glycosyl hydrolase 27 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4L195"/>
    <dbReference type="Ensembl" id="ENSEAST00005001330.2">
        <property type="protein sequence ID" value="ENSEASP00005001172.2"/>
        <property type="gene ID" value="ENSEASG00005000986.2"/>
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    <dbReference type="GeneTree" id="ENSGT00390000008751"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
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        <property type="project" value="GOC"/>
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    <dbReference type="GO" id="GO:0004557">
        <property type="term" value="F:alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008456">
        <property type="term" value="F:alpha-N-acetylgalactosaminidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019377">
        <property type="term" value="P:glycolipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0016139">
        <property type="term" value="P:glycoside catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0009311">
        <property type="term" value="P:oligosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14792">
        <property type="entry name" value="GH27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000025">
        <property type="entry name" value="Alpha-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000070">
        <property type="entry name" value="Alpha-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
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    <dbReference type="InterPro" id="IPR002241">
        <property type="entry name" value="Glyco_hydro_27"/>
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    <dbReference type="InterPro" id="IPR000111">
        <property type="entry name" value="Glyco_hydro_27/36_CS"/>
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    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035373">
        <property type="entry name" value="Melibiase/NAGA_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452">
        <property type="entry name" value="ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452:SF25">
        <property type="entry name" value="ALPHA-N-ACETYLGALACTOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16499">
        <property type="entry name" value="Melibiase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17450">
        <property type="entry name" value="Melibiase_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00740">
        <property type="entry name" value="GLHYDRLASE27"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00512">
        <property type="entry name" value="ALPHA_GALACTOSIDASE"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-0458">Lysosome</keyword>
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    <keyword evidence="16" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040490162" description="Alpha-galactosidase" evidence="16">
        <location>
            <begin position="18"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha galactosidase A C-terminal" evidence="17">
        <location>
            <begin position="311"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036913"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048850"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU361168"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF17450"/>
        </source>
    </evidence>
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            <dbReference type="Ensembl" id="ENSEASP00005001172.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="411" mass="46557" checksum="9C0434CE8F201C6C" modified="2023-09-13" version="1">MLLKTVLLLALVDHVLLLENGLLQKPPMGWLAWERFRCNIDCDEDPKNCISERLFMEMADRLAQDGWRDLGYTYLNIDDCWIGGRDAKGRLVPDPKRFPNGIDFLADYAHSLGLKLGIYSDLGNFTCMGYPGTTLDKVVQDAQTFAEWKVDMLKLDGCFSTPEERAKGYPKMAAALNATGRPIAFSCSWPAYEGGLPPQVNYSLLADICNLWRNYDDIQDSWMSVLSILNWFVEHQDILQPVAGPGHWNDPDMLLVGNYGLSFEQAQSQMALWTVLAAPLFMSTDLRTISAQNMDILQNPLMIKINQDPLGIQGRRILMEKSHIEVFMRPLANEASALVFFSRRTDMPYRYHSSLTHLNFTGSNVYEAQNVYTGDIISGLRAETNFTVVINPSGVVMWYLYPVRKLGMPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L403</accession>
    <name>A0A8C4L403_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ataxin 7 like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">ATXN7L2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_044749595.2"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSEAST00005004316.2">
        <property type="protein sequence ID" value="ENSEASP00005003934.1"/>
        <property type="gene ID" value="ENSEASG00005002984.2"/>
    </dbReference>
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    <dbReference type="CTD" id="127002"/>
    <dbReference type="GeneTree" id="ENSGT00940000159736"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052237">
        <property type="entry name" value="Ataxin-7-like_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013243">
        <property type="entry name" value="SCA7_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15117">
        <property type="entry name" value="ATAXIN 7 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15117:SF5">
        <property type="entry name" value="ATAXIN-7-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08313">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51505">
        <property type="entry name" value="SCA7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCA7" evidence="2">
        <location>
            <begin position="262"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="491"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="563"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="399"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="493"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="573"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="601"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="753" mass="80193" checksum="B822F29EAE8B1E4B" modified="2022-01-19" version="1">MAVRERAAAAMAALERRVPSLDDFAGQSWSSWVERADLLVTDGAELEESNKSTKKLDAMTLIKEDMSIFGHCPAHDDFYLVVCNHCSQVVKPQAFQKHCERRHGPLSKLYARAPPPPPAPASSQKCHVVNGQGPACRAPGSTKTSSREKGQGSRSRGHQPPEKTQKDNLCLFVPVVNLEKMSSLPKPDGHGIRVAPPSAFLSQPGGLTKDSPGKNPMAPPSKEPPGRESIEMNPSEGPSHRTEGSPTEKEASGVRLPPKTHRKMARKECDLNRQCGVINPETKKICTRLLTCKIHSVHQRREVQGRAKDFDVLVAELKANSRKGESPKEKSPGRKEPALERPSQEPPSSVPVVAAAAVPSSTFSARAKQTYPYCALPRSRASSESELDDDGPCGGDGDPGLFPFPLPRGGAQASSEESEEEGTSDDLHLPPDCHYATRPPRPQAFCTFGSRLVSPGCYVFSRRLDRFCSALSSMLERHLSSHMWKKIPPAAEPPSHLVSSSLSAPLSPSSTGSCPRLPGPTPRPACPASTPPTKDSLVPSYPAGSPSVAAACSQAECMGGSQAITSPLPANTPSPSFSKLPPSKASKSSKGKDGAEVEAPSRKRKLSPGPTTFKRTCIMEPTGKGKPGCRGLSTKTKTALGMGLNGTVGPRVKRAGPLDCRGSPHPPPTPVKASQLDNRGAAGHPAKALPTNCLSEEEVAKKRKNLATYCRPVKAKHCQAGTPADAACSVRRKKPGPALAFEEKCSTLKSKAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L440</accession>
    <name>A0A8C4L440_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Voltage-dependent P/Q-type calcium channel subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">CACNA1A</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="22">Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1A gives rise to P and/or Q-type calcium currents.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
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            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Membrane</location>
            <topology evidence="22">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1A subfamily.</text>
    </comment>
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        <property type="component" value="Chromosome 20"/>
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        <property type="term" value="C:cell projection"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="GOC"/>
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        <property type="term" value="C:voltage-gated calcium channel complex"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:syntaxin binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:cellular response to amyloid-beta"/>
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    <dbReference type="GO" id="GO:0007268">
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    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
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    <dbReference type="GO" id="GO:0007204">
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    <feature type="transmembrane region" description="Helical" evidence="25">
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    <feature type="transmembrane region" description="Helical" evidence="25">
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    <feature type="transmembrane region" description="Helical" evidence="25">
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        <ligand>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00037936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU003808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="SMART" id="SM01062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005376.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="2513" mass="282217" checksum="20C80D08747EF823" modified="2023-09-13" version="2">MARFGDEMPARYGGGGSGAAAGVVVGAGGGRGAGGSRQGGQPGAQRMYKQSMAQRARTMALYNPIPVRQNCLTVNRSLFLFSEDNVVRKYAKKITEWPYPFPKPGGATIIANCIVLALEQHLPDDDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFAFHKGSYLRNGWNVMDFVVVLTGILATVGTEFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIFAIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPYWEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYMEWISKAEEVILAEDETDVEQRLPFDALRRATIKKSKTDLLHPEEAEDQLADIASVGSPFARASIKSAKLENSTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALNTLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSSFNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTNFDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVSPLSAANMSIAVKEQQKNQKPAKSVWEQRTSEMRKQNLLASREALYSEMDPDERWKAAYARHLRPDMKTHLDRPLVVDPQENRNNNTNKSRAAEPAGESRARAPSRADDLLRKPRPHERARDAGRPRVPGRAPGLDGRPRARARAQPPGALRPRVGPARARGRPGAAGGLGGRSERGGPGDAPRRRAPRPGGSRDGRGGSPRTGADGEPGAEDKAERRADGEGPEGGERRRRHRHGPAAHNADARREDRERRHRRRRENQGPGVPVPGPNLSTTRPIQQDLGRPDPPLAEDIDNMKNNKLATAEAAGPHASLGPAGPPPSPAKMGNSTEPGVAAATPQNTASRRAPNNPGTPCNPGPPRTPENSLIVTNPSSTQSNSAKTARRPDHTAVDIPPACPPPLSHTVVQVNKNANPDPLPKKEEEKKEEEDDPGEDGPKPMPPYSSMFILSTTNPLRRLCHYILNLRYFEMCILMVIAMSSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQGAYFRDLWNILDFIVVSGALVAFAFTGNSKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHCTDESKEFEKDCRGKYLLYEKNEVKARDREWKKYEFHYDNVLWALLTLFTVSTGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQYRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFTSLFSLECLLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNNFINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIGIDVEDEDSDEDDFQITEHNNFRTFFQALMLLFRSATGEAWHNIMLSCLSGKPCDKNSGILTPECGNEFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAAWGRMPYPDMYQMLRHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALDIKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKIYAAMMIMEYYRQSKAKKLQAMREEQNRTPLMFQRMEPPSPTQEGGPSQNALPSTQLDPGGGLMAHDSGLKESPSWVTQRAQEMFQKTGTWSPERGPPADMPHSQPDSQSVEMREMGRDGYSDSEHYLPMEGQARAASMPRLPAENQPLTGPPACPPQTISDTSPMKRSASVLGPKARRLDDYSLERVPPEENQRHHQRRRDRGHRASERSLGRYTDVDTGLGTDLSMTTQSGDLPPKERDQERGRPKDRKHRQHHHHHHHHHPPPADKERFAPERPEHGRPRARDQRWSRSPLNIWYQHSAAGPPPAPPDPLHPPAARVLLPRGPAGRRRGPPAAASSSSPRRRGRPGRSPARAEVPGARGRARARGAAARGGPRQQPLARDGAAGPGRSQSPRACRHGGARWPAAEGPPGARLHGYYRGSDYDEAEGPGGRRGRAPGRALRRRPPPRRSSSPRTPRPRAPRRLPGAARPAPPERLLPGARRRPAPRPRRPQRPARSLQRDRRRRLVLAPRTPPGPARAPGSPRAPDSAGSPGRTRRCPRRGPQGRTNHTAKAFELTQPFLENLGENGNLNKKKKTFLKEKRGEKNRFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L6A1</accession>
    <name>A0A8C4L6A1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">inorganic diphosphatase</fullName>
            <ecNumber evidence="4">3.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PPA2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4L6A1"/>
    <dbReference type="Ensembl" id="ENSEAST00005006616.2">
        <property type="protein sequence ID" value="ENSEASP00005006041.2"/>
        <property type="gene ID" value="ENSEASG00005004477.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017004"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071344">
        <property type="term" value="P:diphosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051881">
        <property type="term" value="P:regulation of mitochondrial membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00412">
        <property type="entry name" value="pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.80.10:FF:000005">
        <property type="entry name" value="Pyrophosphatase (inorganic) 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.80.10">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008162">
        <property type="entry name" value="Pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036649">
        <property type="entry name" value="Pyrophosphatase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10286">
        <property type="entry name" value="INORGANIC PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00719">
        <property type="entry name" value="Pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50324">
        <property type="entry name" value="Inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00387">
        <property type="entry name" value="PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="85"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006041.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="388" mass="43621" checksum="966FB438A297C557" modified="2023-09-13" version="2">MIVRNTQGRYHVPLQREHVRARRDTCALLGCTGQGFVEDWHSRRSLTVSCCTSGQGHVFARRPDGYATGTDAVVCARPPSLHVGRDLAQRRTERGPSGPHPYRERPVRTAGAGGAVRDAGAEDAAGQYVSPFHDIPLKVDSTENLFNMVVEVPRWTNAKMEIATEEPLNPIKQDLKDGKPRFVANIFPHKGYIWNYGALPQTWEDPHRKEKSTDCCGDNDPIDVCEIGSKVLSRGEVIRVKILGILALIDQSETDWKLIAINVNDPEASKFHDIDDVKKYKPGYLEATLNWFRFYKVPEGKPENRFAFNGEFKDKAFALGVIKSTHECWRALLMKTCDGGAINCTNVQLCDSPFRCTQEEARSLVESMSSSVNKDGEEDEQMWHFLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L7L7</accession>
    <name>A0A8C4L7L7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">CCA tRNA nucleotidyltransferase 1, mitochondrial</fullName>
            <ecNumber evidence="22">2.7.7.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">TRNT1</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <reaction evidence="21">
            <text>a tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
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            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
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        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:14434"/>
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    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a tRNA with a 3' CCA end + 2 CTP + ATP = a tRNA with a 3' CCACCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:76235"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="Rhea" id="RHEA-COMP:18655"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
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            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="ChEBI" id="CHEBI:195187"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
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    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 24">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family.</text>
    </comment>
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    <dbReference type="AlphaFoldDB" id="A0A8C4L7L7"/>
    <dbReference type="SMR" id="A0A8C4L7L7"/>
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        <property type="protein sequence ID" value="ENSEASP00005004752.2"/>
        <property type="gene ID" value="ENSEASG00005003577.2"/>
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        <property type="component" value="Chromosome 21"/>
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        <property type="term" value="C:cytosol"/>
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        <property type="entry name" value="PolyA_pol_RNAbd"/>
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                <person name="Liu"/>
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        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="21781" checksum="C400463FC6F2A183" modified="2022-01-19" version="1">MAGLVPRPAKGYRVVLLGSVAVGKTALATQFACGSFPEQCEPSVEELFSKVIEVNAAPALLEIVDTVGAEHLVTLKDLYIKNSDGFVVLYSVCSEASFEAVRPLRERMGRLRGPKAVPLVLVGTKADLDAERQVLTAQGRALAREWRCPFLEVTAKSKLMVDQVFTQVVREMEALAPPEEEVPAVPTNAQDTWPSERFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LBY3</accession>
    <name>A0A8C4LBY3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">N-sulfoglucosamine sulfohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SGSH</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sulfatase family.</text>
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        <property type="nucleotide sequence ID" value="XM_014863856.3"/>
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        <property type="gene ID" value="ENSEASG00005005113.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106845569"/>
    <dbReference type="KEGG" id="eai:106845569"/>
    <dbReference type="CTD" id="6448"/>
    <dbReference type="GeneTree" id="ENSGT00390000013080"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
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    <dbReference type="GO" id="GO:0043202">
        <property type="term" value="C:lysosomal lumen"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016250">
        <property type="term" value="F:N-sulfoglucosamine sulfohydrolase activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008484">
        <property type="term" value="F:sulfuric ester hydrolase activity"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:determination of adult lifespan"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:glycosaminoglycan catabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:heparan sulfate proteoglycan catabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061744">
        <property type="term" value="P:motor behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd16027">
        <property type="entry name" value="SGSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000026">
        <property type="entry name" value="N-sulphoglucosamine sulphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032506">
        <property type="entry name" value="SGSH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024607">
        <property type="entry name" value="Sulfatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000917">
        <property type="entry name" value="Sulfatase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-SULFATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43108:SF6">
        <property type="entry name" value="N-SULPHOGLUCOSAMINE SULPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16347">
        <property type="entry name" value="SGSH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00884">
        <property type="entry name" value="Sulfatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SULFATASE_2"/>
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    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040433402" evidence="6">
        <location>
            <begin position="21"/>
            <end position="523"/>
        </location>
    </feature>
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        <location>
            <begin position="55"/>
            <end position="348"/>
        </location>
    </feature>
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            <begin position="439"/>
            <end position="492"/>
        </location>
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    </evidence>
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        </source>
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        </source>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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</entry>
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    <accession>A0A8C4LCM0</accession>
    <name>A0A8C4LCM0_EQUAS</name>
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        <submittedName>
            <fullName evidence="8">SURP and G-patch domain containing 2</fullName>
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    <gene>
        <name evidence="8" type="primary">SUGP2</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="H."/>
                <person name="Guo"/>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014865162.3"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
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    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="SUGP1/2"/>
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    <dbReference type="InterPro" id="IPR035967">
        <property type="entry name" value="SWAP/Surp_sf"/>
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    <dbReference type="PANTHER" id="PTHR23340">
        <property type="entry name" value="ARGININE/SERINE RICH SPLICING FACTOR SF4/14"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23340:SF2">
        <property type="entry name" value="SURP AND G-PATCH DOMAIN-CONTAINING PROTEIN 2"/>
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    <dbReference type="Pfam" id="PF01585">
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SURP motif" evidence="6">
        <location>
            <begin position="786"/>
            <end position="829"/>
        </location>
    </feature>
    <feature type="domain" description="G-patch" evidence="7">
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            <begin position="1019"/>
            <end position="1065"/>
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            <begin position="696"/>
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            <begin position="998"/>
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    <evidence type="ECO:0000313" key="9">
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    <sequence length="1090" mass="120751" checksum="8F364B7C78165AEB" modified="2023-09-13" version="2">MAARRIAQETFDAVLQEKANRYHMDPSSEAVGEALQFKAQDLLRTVPRARADMYDDIHSDSRYTLGGSVAHSRETAREGLRSDVFPGPSFRSSNPSVSDDNYFRKECGRDLEFSHPDSRDQVFGHRKLGHFRSQDWKFALRGSWEQDFGHPVSQESSWSQEYNFGPSALLGDFGSSRLIEKECLEKESRDFDVDRPGEADSVLRGSGQVQGRGQGLNIIDQEGALLGKGESQGLLTPKVGVGKLVTLRSVSMKKVPTINRITPKTQGTNQIQKTTPSPDVTLGTNPGTEDIQFPTQKIPLGLNLKDIRLPRRKMSFDLIDKSDVFSRFGIEIIKWAGFHTIKDDVKFSQLFQTLFELETETCAKMLASFKCSLKPEHRDFCFFTIKFLKHSALKTPRVDNEFLNMLLDKGAVKTKNCFFEIIKPFDKYIMRLQDRLLKSVTPLLMACNAYELSVKMKTLSNPLDLAVALETTNSLCRKSLALLGQTFSLASSFRQEKILEAVGLQDIAPSPAAFPNFEDSTLFGREYIDHLKAWLVSSGCPLQVKKAEPEPTQDEKTVPPTKPEIEAKAPCGLSDAVPQRADHKVVDTIDQLVTRVVGGSLSPKERALLKEDPAYWFLSDDSSLEYKYYKLKLAETQRMSQTLPGVDQKPMAAECAVRAMLYARAVRSLKKRLLPGRRRGLLRAQGLRGWKMRRATTGTQTLLSSGTRLKHHSRQATGSLQGKLPQPDGNDTAKDCPPDPAGPSSRDPSPEASGPSPKPGGMDVSEAPQTSSPCLSAEIDTKTMETAEKLAKFVAQVGPEIEQFSIENSTDNPDLWFLHDQNSSAFKFYRKKVFELCPSICFTSSPLNLHAGESTLERVEGEEEFEDEPLQHEAELESPEVMPEEEEEDDDEEEDDDEEGGDGALAPGGASRPAGGAAKSEGSEGSPTADGLPSEVAEDGPAGTPALSQASSGACFPRKRVSSKSLKVGMIPAPKRLCLIQEPKVHEPVRIAYDRPRGRPMSKKKKSKDFDFAQQKLTDKNLGFQMLQKMGWKEGHGLGSCGKGIREPVSVGTASEGEGLGADGQEHKEDTFDVFRQRMMQMYRHKRANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LE42</accession>
    <name>A0A8C4LE42_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Kinesin light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">KLC2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Kinesin is a microtubule-associated force-producing protein that play a role in organelle transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Oligomeric complex composed of two heavy chains and two light chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the kinesin light chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LE42"/>
    <dbReference type="Ensembl" id="ENSEAST00005007266.2">
        <property type="protein sequence ID" value="ENSEASP00005006645.2"/>
        <property type="gene ID" value="ENSEASG00005004656.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161973"/>
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        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000003">
        <property type="entry name" value="kinesin light chain isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002151">
        <property type="entry name" value="Kinesin_light"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015792">
        <property type="entry name" value="Kinesin_light_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783">
        <property type="entry name" value="KINESIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45783:SF2">
        <property type="entry name" value="KINESIN LIGHT CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00381">
        <property type="entry name" value="KINESINLIGHT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01160">
        <property type="entry name" value="KINESIN_LIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 10" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="250"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="292"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="154"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="506"/>
            <end position="555"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="567"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="95"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
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            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
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            <begin position="546"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="609"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004245"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00009622"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006645.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="630" mass="69461" checksum="2663764BD9751F42" modified="2023-09-13" version="2">MDSSERGGAAMATMVLPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLVAHEAGDAEPGSQERCVLLRRSLEAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQQKLQRSEQAVAQLEEEKQHLLFMSQIRKLDDDTSPNEEKGDVPKDSLDDLFPNEEEQGPAPSPGGGDVAAQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYATRLGPDDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNGDNKPIWMHAEEREESKDKRRDSTPYGEYGSWYKACKVDSPTVNTTLRSLGALYRRQGKLEAAHTLEDCASRSRKQGLDPASQTKVVELLKDGSGVRGDRRGSRDVAGGARSESDLEEAGPAAEWSGDGSGSLRRSGSFGKLRDALRRSSEMLVKKLQGGSPQESPNPRMKRASSLNFLNKSVEEPVQPGGTGLSDSRTLSSSSMDLSRRSSLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LFI6</accession>
    <name>A0A8C4LFI6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Homeobox protein goosecoid-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Homeobox protein goosecoid-like</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GSC2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">May have a role in development. May regulate its own transcription. May bind the bicoid consensus sequence TAATCC.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family. Bicoid subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LFI6"/>
    <dbReference type="Ensembl" id="ENSEAST00005009264.2">
        <property type="protein sequence ID" value="ENSEASP00005008499.2"/>
        <property type="gene ID" value="ENSEASG00005006115.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162536"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000223">
        <property type="entry name" value="Goosecoid homeobox 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051440">
        <property type="entry name" value="Goosecoid-like_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46643:SF1">
        <property type="entry name" value="HOMEOBOX PROTEIN GOOSECOID-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46643">
        <property type="entry name" value="HOMEOBOX PROTEIN GOOSECOID-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="123"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="125"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="97"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="26"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00078927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008499.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="204" mass="21662" checksum="50589CC2C1188842" modified="2023-09-13" version="2">MAAAGGAASRRGPGQPCPFSIEHILSSLPERSAPARAARPPQPAGRQSPAESGEREAPEAAPCACCCCCGPRAAPRGPSEAAAGLGARLPWPLRLGPSAPLPLAAPTGGSGALPGAGGPGPQRRTRRHRTIFSEEQLQALEALFVQNQYPDVGTRERLAGRIRLREERVEVWFKNRRAKWRHQKRASASARLLPGPKKPPKESC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LI70</accession>
    <name>A0A8C4LI70_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lactotransferrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">LTF</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. Shows a preferential cleavage at -Arg-Ser-Arg-Arg-|- and -Arg-Arg-Ser-Arg-|-, and of Z-Phe-Arg-|-aminomethylcoumarin sites.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the transferrin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LI70"/>
    <dbReference type="Ensembl" id="ENSEAST00005012516.2">
        <property type="protein sequence ID" value="ENSEASP00005011514.2"/>
        <property type="gene ID" value="ENSEASG00005008098.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156055"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042581">
        <property type="term" value="C:specific granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001530">
        <property type="term" value="F:lipopolysaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140912">
        <property type="term" value="F:membrane destabilizing activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019731">
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8C4LJS9_EQUAS</name>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Guo"/>
                <person name="Y."/>
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                <person name="Zhao"/>
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                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
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                <person name="Y."/>
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                <person name="Wang"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </evidence>
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    </evidence>
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                <person name="X. and Zhong"/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <source>
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    </evidence>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4LKP2"/>
    <dbReference type="Ensembl" id="ENSEAST00005014478.2">
        <property type="protein sequence ID" value="ENSEASP00005013332.2"/>
        <property type="gene ID" value="ENSEASG00005009288.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158519"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14153">
        <property type="entry name" value="PK_KSR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000107">
        <property type="entry name" value="kinase suppressor of Ras 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000034">
        <property type="entry name" value="Kinase suppressor of Ras 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000031">
        <property type="entry name" value="Kinase suppressor of Ras 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000010">
        <property type="entry name" value="Putative kinase suppressor of Ras 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025561">
        <property type="entry name" value="KSR_SAM-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046861">
        <property type="entry name" value="SAM_KSR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046933">
        <property type="entry name" value="SAM_KSR1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050167">
        <property type="entry name" value="Ser_Thr_protein_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257:SF775">
        <property type="entry name" value="KINASE SUPPRESSOR OF RAS 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23257">
        <property type="entry name" value="SERINE-THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13543">
        <property type="entry name" value="SAM_KSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20406">
        <property type="entry name" value="SAM_KSR1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="20">
        <location>
            <begin position="412"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="657"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="239"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="496"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="259"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="496"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="507"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="521"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="541"/>
            <end position="553"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005013332.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="940" mass="106354" checksum="F7010281BC31EF6D" modified="2023-09-13" version="2">MDEENMTKSEEQQPLSLQKALQQCELVQNMIDLSISNLEGLRTKCATSNDLTQKEIRTLESKLVKYFSRQLSCKKKVALQERNAELDGFPQLRHWFRIVDVRKEVLEEITPGQLSLEDLLEMTDEQVCETVEKYGANREECARLNASLSCLRKVHMSGGNLSKQDWTIQWPTTETGKENNPVCPPEPTPWIRTHLSQSPRVQSKCVQHYCHASPTPGAPVYTHVDRLTVDAYPGLCPPPPLESGHRSLPPSPRQRHAVRTPPRTPNIVTTVTPPGTPPMRRKNKLKPPGTPPPSSRKLIHLIPGFTALHRSKSHEFQLGHRIDEAHTPKAKKKSKPLNLKIHSSVGSCENIPSQQRSPLLSERSLRSFFVGHAPFLPSTPPVHTEANFSANTLSVPRWSPQIPRRDLGNSIKHRFSTKYWMSQTCTVCGKGMLFGLKCKNCKLKCHNKCTKEAPPCHLLIIHRGARLVRTESVPCDINNPLRKPPRYSDLHISQTLPKTNKINKPDSSSNPSSTTSSTPSSPAPPLPPSATPPSPLHPSPQCTRQQKNFSLPGTSVLGETQRVRRWRDVVPVPETPTRAPQVILHPVTSNPILEGNPLLQIEVEPTSENEEGHDEAEESEDDFEEMNLSLLSARSFPRKASQTSIFLQEWDIPFEQLEIGELIGKGRFGQVYHGRWHGEVAIRLIDIERDNEEQLKAFKREVMAYRQTRHENVVLFMGACMSPPHLAIITSLCKGRTLYSVVRDAKIVLDVNKTRQIAQEIVKGMGYLHAKGILHKDLKSKNVFYDNGKVVITDFGLFSISGVLQAGRREDKLRIQNGWLCHLAPEIIRQLSPDTEEDKLPFSKHSDVFALGTIWYELHAREWPFKTQPAEAIIWQMGTGMKPNLSQIGMGKEISDILLFCWAFEQEERPTFTKLMDMLEKLPKRNRRLSHPGHFWKSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LL28</accession>
    <name>A0A8C4LL28_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Plexin-B1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">PLXNB1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Receptor for SEMA4D. Plays a role in GABAergic synapse development. Mediates SEMA4A- and SEMA4D-dependent inhibitory synapse development. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the plexin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014682108.2">
        <property type="nucleotide sequence ID" value="XM_014826622.3"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="GTPase Activation - p120gap, domain 1"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="entry name" value="PLEXIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PLEXIN-B1"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="TIG_plexin"/>
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        <property type="match status" value="3"/>
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        <property type="entry name" value="E set domains"/>
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    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040376709" description="Plexin-B1" evidence="18">
        <location>
            <begin position="20"/>
            <end position="2137"/>
        </location>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="689"/>
            <end position="838"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="853"/>
            <end position="884"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1378"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1886"/>
            <end position="1910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="822"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1890"/>
            <end position="1899"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010297"/>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
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            <dbReference type="ARBA" id="ARBA00057668"/>
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    <evidence type="ECO:0000256" key="15">
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    <evidence type="ECO:0000256" key="16">
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            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005013008.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="2137" mass="233519" checksum="6B221ED06A3E7A89" modified="2023-09-13" version="2">MPALGPALLQALWAGWVLTLQPPPPAAFTPNGTQLQHMARDPTSGTLYLGATNFLFQLSPGLQLEATVSTGPVLDSRDCLPPVMPDECPQAQPTNNLNQLLLVSPGALLVCGSVHQGVCEQRRLGQLGQLLLRPERPGDTQYVAANDPAVSTVGLVAQGLAGEPLLFVGRGYTSRGVGGGIPPITTRALRPLDPQAAFSYEETAKLAVGRLSEYSHHFVSAFAHGASAYFLFLRRDLQAQSRAFRAYVSRVCLRDQHYYSYVELPLACQGGRYGLIQAAAVATSGEVARREVLFAAFSSASPPAVGRPPSAAAGASGASALCAFPLDEVDHLANRTRDACYTREGRAEDGAEVAYIEYDVNSDCAQLPVDTLDAYPCGSDHTPSPMASRVPLEATPVLEWPGVQLTAVAVTMEDGHTIAFLGDSQGQLHRVFLGPGSDGHPYSTQSIQQGSAVSRDLTFDGAFEHLYVMTQSTLLKVPVASCAQHLDCASCLAHRDPYCGWCVLLGRCSRRSECSRGQGPERWLWSFQPELGCLQVADMSPANMSREERREVFLSVPDLPPLWPGESYSCHFGEHQSPALLTSSGVMCPSPDPSEAPALPRGDDHVSVSVELRFGTVMIAKASLSFYDCVAVTKLRPSAQCQACVSSRWGCNWCVWQHLCTHKASCDAGPMVVSQQSLLLSPAPPAGVAPTPLPPAAPKATVIPAPDTLPVEHDAPSTATASDIPPRARPSLLSPWGPWAGPGPIPASASTESPLHEELPPPSPPNRPGTTVTVPTDFGPMATPEDLLASHPTPSDAAALPPAPADPGPEALPSVIPLDQHPGTAPATTFPGATGSTKPALDWLMREVGELPEADEWTGGDTPAFSTSTLLSGDGDSAEHEGPPAPLILLSSLDYQYDTPGLWELEEVTWGASSCPCVESIQGSMLMPVHVEREVRLLGRNLHLFQDSPGDNECVMELEGHEVVVEAQVKCEPPPDTRCHVTCQQHQLSYEALQPELRVGLFLRRAGHLRVDSADGLHVVLYDCSVGHEDCSRCQTAMPQYGCVWCKGERPRCVAQEACAEAEAVATQCPAPLIHSVEPLTGPIDGGTRVTIRGSNLGQHLQDVQDTVRVAGIPCAVDAREYEVSSSLVCITEASGEEVADAVTVEVLGRGRGISEHDFAYQDPKVHSIFPARGPRAGGTRLTLHGSKLLTGRLEDIRVVVGDQPCHLLLEQQAEQLQCETSPHPAPAMLPVAVWFGATERRLQHSQFEYTSDPNITSAGPTKSFLSGGREIWVRGQNLDVVQMPRIRVTVALRALQLSQGLGRRRRVIPETPCSPGASCGSHHFEEPCHVNSSQLITCRTPALPVLPEDPWVRVEFILDSLVFDFATLNPTPFSYEADPTLQPLNPEDPTTPFRHKPGSVLSVEGENLDLAMSKEEVVAMIGDGPCVVKTLTRHHLYCEPPVDQPLPRHHALREAPDTLPEFTVQMGNLRFSLGHVQYDGESPVAFPMAAQVGLGVGTSLLALGVIIIVLMYRRKSKQALRDYKKVQIQLENLESSVRDRCKKEFTDLMTEMTDLTSDLLGSGIPFLDYKVYAERVFFPGHQESPLHRDLGVPESRRPTVEQGLGQLSNLLNSKLFLTKFIHTLESQRTFSARDRAYVASLLTVALHGKLEYFTDILRTLLSDLVAQYVAKNPKLMLRRTETVVEKLLTNWMSICLYTFVRDSVGEPLYMLFRGIKHQVDKGPVDSVTGKAKYTLNDNRLLREDVEYRPLTLNALLAVGPGAGEAQGVPVKVLDCDTISQAKEKMLDQLYKGMPLAQRPDPRTLDVEWRSGVAGHLILSDEDVTSEVQGLWRRLNTLQHYKVPDGATVALVPCLTKHVLRENQDYVPGERTPMLEDIDEGGIRPWHLVKPSEEPEPPRPRRGSLRGGERERAKAIPEIYLTRLLSMKGTLQKFVDDLFQVILSTSRPVPLAVKYFFDLLDEQAQQHGISDQDTVHIWKTNSLPLRFWINIIKNPQFVFDVQTSDNMDAVLLVIAQTFMDACTLADHKLGRDSPINKLLYARDIPRYKRMVERYYADIRQTIPASDQEMNSILAELSRNYSGDLGARVALHELYKYINKYYDQIITALEEDGTAQKMQLGYRLQQIAAAVENKVTDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LNK8</accession>
    <name>A0A8C4LNK8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Sterile alpha motif domain containing 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SAMD3</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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                <person name="Huang"/>
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                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014711651.1">
        <property type="nucleotide sequence ID" value="XM_014856165.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LRL4"/>
    <dbReference type="Ensembl" id="ENSEAST00005017346.2">
        <property type="protein sequence ID" value="ENSEASP00005015958.1"/>
        <property type="gene ID" value="ENSEASG00005011090.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106840591"/>
    <dbReference type="KEGG" id="eai:106840591"/>
    <dbReference type="GeneTree" id="ENSGT00940000162730"/>
    <dbReference type="OMA" id="LWNPPEV"/>
    <dbReference type="OrthoDB" id="10042731at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990080">
        <property type="term" value="F:2-phenylethylamine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001594">
        <property type="term" value="F:trace-amine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001662">
        <property type="term" value="P:behavioral fear response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007635">
        <property type="term" value="P:chemosensory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007606">
        <property type="term" value="P:sensory perception of chemical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd15312">
        <property type="entry name" value="7tmA_TAAR2_3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000030">
        <property type="entry name" value="trace amine-associated receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050569">
        <property type="entry name" value="TAAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009132">
        <property type="entry name" value="TAAR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249">
        <property type="entry name" value="HISTAMINE RECEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24249:SF220">
        <property type="entry name" value="TRACE AMINE-ASSOCIATED RECEPTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01830">
        <property type="entry name" value="TRACEAMINER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="34"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="104"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="149"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="205"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="294"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="50"/>
            <end position="313"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005015958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="347" mass="38998" checksum="3D8416D32F3C2DD8" modified="2022-01-19" version="1">MNSPDIGNTQEVQFCFALVNNSCPRNVRSVLSVCAMYVVMIGAIVMTMLGNLVVIISIAHFKQLHSPTNFLILSMATTDFLLSCVVMPFSMIRSIESCWYFGDLFCKVHSCCDIMLCTTSIFHLCFISVDRYYAVCDPLHYVTKITIPVIEVFLFISWSIPIFFALGLVFSELNVIGAEDFVAAIDCTGLCVLIFNKLWGVLASFIAFFLPGTVMMGIYIYIFTVAQKHAKQIGTGPLMKQVRSESKMKAASKKESKATKTLGIVVGVFVLCWLPFFVLTITDPFINFTTPEDLYNVFLWLGYFNSTFNPIIYGMFYPWFRRALRVIVSGRVFCPDSSTLSLFPAHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LSI8</accession>
    <name>A0A8C4LSI8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">MAGUK p55 subfamily member 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Discs large homolog 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Protein MPP3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">MPP3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Participates in cell spreading through the phosphoinositide-3-kinase (PI3K) pathway by connecting CADM1 to DLG1 and the regulatory subunit of phosphoinositide-3-kinase (PI3K). Stabilizes HTR2C at the plasma membrane and prevents its desensitization. May participates in the maintenance of adherens junctions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with HTR2C; this interaction stabilizes the receptor at the plasma membrane and prevents the desensitization of the HTR2C receptor-mediated calcium response. Interacts with HTR2A. Interacts with HTR4. Interacts (via PDZ domain) with CADM1 (via C-terminus)Interacts (via PDZ domain) with CADM1; this interaction connects CADM1 with DLG1. Interacts (via Guanylate kinase-like domain) with PALS1. Interacts with DLG1 (via N-terminus); this interaction connects CADM1 with DLG1 and links CADM1 with the regulatory subunit of phosphoinositide-3-kinase (PI3K) by forming a multiprotein complex and participates in cell spreading.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Adherens junction</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LSI8"/>
    <dbReference type="Ensembl" id="ENSEAST00005015800.2">
        <property type="protein sequence ID" value="ENSEASP00005014542.2"/>
        <property type="gene ID" value="ENSEASG00005010126.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157190"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06799">
        <property type="entry name" value="PDZ_MPP3-MPP4-MPP7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12039">
        <property type="entry name" value="SH3_MPP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000215">
        <property type="entry name" value="MAGUK p55 subfamily member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000046">
        <property type="entry name" value="MAGUK p55 subfamily member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.650">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014775">
        <property type="entry name" value="L27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004172">
        <property type="entry name" value="L27_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036892">
        <property type="entry name" value="L27_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050716">
        <property type="entry name" value="MAGUK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035604">
        <property type="entry name" value="MPP3_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23122">
        <property type="entry name" value="MEMBRANE-ASSOCIATED GUANYLATE KINASE MAGUK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02828">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00569">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101288">
        <property type="entry name" value="L27 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51022">
        <property type="entry name" value="L27"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="L27" evidence="19">
        <location>
            <begin position="6"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="L27" evidence="19">
        <location>
            <begin position="61"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="18">
        <location>
            <begin position="137"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="16">
        <location>
            <begin position="226"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="17">
        <location>
            <begin position="377"/>
            <end position="562"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00066116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00079626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00080907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014542.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="577" mass="65412" checksum="468FFDC13D78E3FD" modified="2023-09-13" version="2">MPVLSEDSGLHETLALLTSQLRPDSNHKEEMGFLRDVFSEKSLSYLMKIHEKLRYYETQSPTPVLHSAVALAEDVMEELQAASAHSDERELLQLLSTPHLRAMLMVHDTVAQKNFDPVLPPLPDNIEEDFEEESVKIVRLVKNKEPLGATIRRDEHSGAVVVARIMRGGAADRSGLVHVGDELREVNGIAVLHKRPDEISQILAHSQGSITLKIIPATQEEDRLKESKVFMRALFHYNPREDRAIPCQEAGLPFQRRQILEVVSQDDPTWWQAKRVGDTNLRAGLIPSKQFQERRLSYRRATGTLPSPQSLKKPTTCDCEGYLRGHYVAGLRRSFRLGRRERLGSPQEGKTASVAESPELLTYEEVTRYQHQPGERPRLVVLIGSLGARLHELKQKVVAENPQHFGVAVPHTTRPRKSHEKEGVEYHFVSKQAFEADLHHNRFLEHGEYKENLYGTSLDAIQAVMAKNKVCLVDVEPDALQQLRTSEFKPYVVFVKPAIQEKRKTPPMSPACEDTASPLDEEQQEMVASAAFIEQHYGHLVDAVLVKEDLQSAHSQLRVLLEKLSQDSHWVPISWVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LTE5</accession>
    <name>A0A8C4LTE5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Kelch domain-containing protein 8B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">KLHDC8B</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in pinching off the separated nuclei at the cleavage furrow and in cytokinesis. Required for mitotic integrity and maintenance of chromosomal stability. Protects cells against mitotic errors, centrosomal amplification, micronucleus formation and aneuploidy. Plays a key role of midbody function involving abscission of the daughter cells during cytokinesis and appropriate chromosomal and nuclear segregation into the daughter cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Midbody</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014695884.1">
        <property type="nucleotide sequence ID" value="XM_014840398.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LTE5"/>
    <dbReference type="Ensembl" id="ENSEAST00005017458.2">
        <property type="protein sequence ID" value="ENSEASP00005016062.2"/>
        <property type="gene ID" value="ENSEASG00005011155.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106830682"/>
    <dbReference type="KEGG" id="eai:106830682"/>
    <dbReference type="CTD" id="200942"/>
    <dbReference type="GeneTree" id="ENSGT00940000160815"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110070">
        <property type="term" value="C:cellularization cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045171">
        <property type="term" value="C:intercellular bridge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902410">
        <property type="term" value="P:mitotic cytokinetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140014">
        <property type="term" value="P:mitotic nuclear division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098813">
        <property type="term" value="P:nuclear chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000053">
        <property type="entry name" value="Kelch domain-containing protein 8B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051746">
        <property type="entry name" value="Kelch_domain_containing_8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46260:SF2">
        <property type="entry name" value="KELCH DOMAIN-CONTAINING PROTEIN 8B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46260">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005016062.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="361" mass="38280" checksum="A195FF07A5C4B8ED" modified="2023-09-13" version="2">MATGGSRAFAWQVFPPMPTCRVYGTVAYQDGHLLVLGGCGQAGLPLDTAETLDMASHTWLALAPLPTPRAGAAAVVLGKQVLVMGGVDEGQSPVAAVEAFLADEGRWERRATLPQAAMGVATVERDGMVYALGGMGPDTAPQAQVRVYEPRGDCWLSLPSMPTPCYGASTFLHGNKIYVLGAPIPLAGGRQGKLPVTAFEAFDLEARTWTRHPSLPSRRAFAGCAMAEGSVFSLGGLQQPGPHNFYSRPHFVNTVEMFDLEHGSWTKMPRSLRMRDKRADFVVGSLGNHIVAIGGLGNQPCPLGSVEGFSLARRRWEALPAMPTARCSCSSLQAGHRLFVIGGVAQGPSQAVEALCLRDGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LU51</accession>
    <name>A0A8C4LU51_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Developmental pluripotency associated 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DPPA2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-glutaminyl-[protein] + L-lysyl-[protein] = [protein]-L-lysyl-N(6)-5-L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:54816"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10207"/>
            <dbReference type="Rhea" id="RHEA-COMP:14005"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="ChEBI" id="CHEBI:138370"/>
            <dbReference type="EC" id="2.3.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="23">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="23">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Tetramer of two A chains (F13A1) and two B (F13B) chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transglutaminase superfamily. Transglutaminase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.13" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4LV18"/>
    <dbReference type="Ensembl" id="ENSEAST00005019000.2">
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        <property type="gene ID" value="ENSEASG00005012064.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244939"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003810">
        <property type="term" value="F:protein-glutamine gamma-glutamyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072378">
        <property type="term" value="P:blood coagulation, fibrin clot formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000978">
        <property type="entry name" value="coagulation factor XIII A chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001301">
        <property type="entry name" value="Coagulation factor XIII A chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.260.10:FF:000001">
        <property type="entry name" value="Protein-glutamine gamma-glutamyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000171">
        <property type="entry name" value="protein-glutamine gamma-glutamyltransferase 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
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    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
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    <dbReference type="InterPro" id="IPR050779">
        <property type="entry name" value="Transglutaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002931">
        <property type="entry name" value="Transglutaminase-like"/>
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    <dbReference type="InterPro" id="IPR023608">
        <property type="entry name" value="Transglutaminase_animal"/>
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    <dbReference type="InterPro" id="IPR013808">
        <property type="entry name" value="Transglutaminase_AS"/>
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    <dbReference type="InterPro" id="IPR008958">
        <property type="entry name" value="Transglutaminase_C"/>
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    <dbReference type="InterPro" id="IPR036238">
        <property type="entry name" value="Transglutaminase_C_sf"/>
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    <dbReference type="InterPro" id="IPR001102">
        <property type="entry name" value="Transglutaminase_N"/>
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    <dbReference type="PANTHER" id="PTHR11590:SF42">
        <property type="entry name" value="COAGULATION FACTOR XIII A CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="E set domains"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF49309">
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    </dbReference>
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    </dbReference>
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    <keyword evidence="14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="9 23" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="8 23" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Transglutaminase-like" evidence="25">
        <location>
            <begin position="307"/>
            <end position="400"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="active site" evidence="22">
        <location>
            <position position="315"/>
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        <location>
            <position position="374"/>
        </location>
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        <location>
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        </location>
    </feature>
    <feature type="binding site" evidence="23">
        <location>
            <position position="437"/>
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        <ligand>
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        <location>
            <position position="439"/>
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        <ligand>
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        </ligand>
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        <location>
            <position position="486"/>
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        <ligand>
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        </ligand>
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        <location>
            <position position="491"/>
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        <ligand>
            <name>Ca(2+)</name>
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        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00005968"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <property type="entry name" value="TCR_variable_region"/>
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    <dbReference type="PANTHER" id="PTHR19367:SF41">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-1279">T cell receptor</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040477836" description="Ig-like domain-containing protein" evidence="6">
        <location>
            <begin position="21"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="21"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005016810.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="124" mass="13716" checksum="D539DD7DC573601C" modified="2023-09-13" version="2">MLSAICSVLVIFLIFRGTNGDSVNQTEGPVTLSEGASLTLNCTYQTYSSPLLLWYIQYLKPPQLLLKSSTENQRTENHFQATLIMSDSSFHLRKPSVQMSDSAVYYCALRDTVRGISQSLGSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M027</accession>
    <name>A0A8C4M027_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">FYVE, RhoGEF and PH domain-containing protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Rho/Rac guanine nucleotide exchange factor FGD1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Zinc finger FYVE domain-containing protein 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">FGD1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Ruffle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4M027"/>
    <dbReference type="Ensembl" id="ENSEAST00005020571.2">
        <property type="protein sequence ID" value="ENSEASP00005018956.2"/>
        <property type="gene ID" value="ENSEASG00005013013.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159438"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046847">
        <property type="term" value="P:filopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd15741">
        <property type="entry name" value="FYVE_FGD1_2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01219">
        <property type="entry name" value="PH1_FGD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13236">
        <property type="entry name" value="PH2_FGD1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000061">
        <property type="entry name" value="FYVE, RhoGEF and PH domain containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000279">
        <property type="entry name" value="FYVE, RhoGEF and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000013">
        <property type="entry name" value="FYVE, RhoGEF and PH domain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000102">
        <property type="entry name" value="FYVE, RhoGEF and PH domain-containing protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
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    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
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    <dbReference type="InterPro" id="IPR035941">
        <property type="entry name" value="FGD1-4_PH2"/>
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    <dbReference type="InterPro" id="IPR035939">
        <property type="entry name" value="FGD1_PH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051092">
        <property type="entry name" value="FYVE_RhoGEF_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673">
        <property type="entry name" value="FACIOGENITAL DYSPLASIA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12673:SF79">
        <property type="entry name" value="FYVE, RHOGEF AND PH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DH" evidence="20">
        <location>
            <begin position="372"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="19">
        <location>
            <begin position="589"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="domain" description="FYVE-type" evidence="21">
        <location>
            <begin position="729"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="19">
        <location>
            <begin position="829"/>
            <end position="929"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="701"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="932"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="125"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="173"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="199"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="233"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="273"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="285"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="316"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="335"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053932"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00080752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00081027"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005018956.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="969" mass="107227" checksum="12E1F82C1B90A01C" modified="2023-09-13" version="2">MHGHRAPGGAGPSESEHPTTNPPGATPPACANSDPGASEPGLPVPRGSGSAFGGPLDPQFAGPSDASLGAAPGPRVLPCGPSPQHHRALRFSYHLEGSQPRPGLHQGNRILVKSLSLEPGQSLEPHPEGPQRLRSDPGPPTETPSQRPSPLKRAPGPKPQVPPKPSYLQMPRMPLPPEPIPPPPSRPLPADPRVAKGLAPRSEASPSSAAVSSLIEKFEREPVIVTSDRPTPGLCPGPTEPAMLPQPPPQPPVPQLPEGEASRCLFLLAPGPRDGEKVPNRDSGIDSISSPSNSEETCFVSDDGPPSHSLCPGPPALASVPVALADPYRPGSQEVDSDLEEEDDEEEDEKDREIPVPPMERQESVELTVQQKVFHIANELLQTEKAYVSRLHLLDQVFCARLLEEARNRSSFPADVVHGIFSNICSIYCFHQQFLLPELEKRMEEWDRYPRIGDILQKLAPFLKMYGEYVKNFDRAVELVNTWTERSTQFKVIIHEVQKEEACGNLTLQHHMLEPVQRIPRYELLLKDYLLKLPHGSPDSKDAQKSLELIATAAEHSNAAIRKMERMHKLLKVYELLGGEEDIVSPTKELIKEGHILKLSAKNGTTQDRYLILFNDRLLYCVPRLRLLGQKFSVRARIDVDGMELKESSNLNLPRTFLVSGKQRSLELQARTEEEKKDWVQAINSTLLKHEQTLETFKLLNSTNREDEDTPPNSPNVDLGKRAPTPIREKEVTMCMRCQEPFNSITKRRHHCKACGHAALACLPPQVVCGKCSEFRARLIYDNNRSNRVCTDCYVALHGVPGSSPACSQHTPQRRRSILEKQASVAAENSVICSFLHYMEKGGKGWHKAWFVVPENEPLVLYIYGAPQDVKAQRSLPLIGFEVGPPEAGERPDRRHVFKITQSHLSWYFSPETEELQRRWMAVLGRAGRGDTFCPGPTLSEDREMEEAPVAASGATAEPSEGPQTQDKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M4Y1</accession>
    <name>A0A8C4M4Y1_EQUAS</name>
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        <submittedName>
            <fullName evidence="3">Chromosome 1 open reading frame 52</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="primary">C1orf52</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the UPF0690 family.</text>
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        <property type="gene ID" value="ENSEASG00005014090.2"/>
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        <property type="component" value="Chromosome 16"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="DUF4660"/>
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        <property type="entry name" value="UPF0690 PROTEIN C1ORF52"/>
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    <dbReference type="PANTHER" id="PTHR31833:SF2">
        <property type="entry name" value="UPF0690 PROTEIN C1ORF52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15559">
        <property type="entry name" value="DUF4660"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="73"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="160"/>
            <end position="242"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="211"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="232"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="242" mass="26042" checksum="B55A80B43C6B1DF9" modified="2022-01-19" version="1">MGAAPPPASIPPRGSGISAQPPGGASPERICEPGGGANGGAGPGAASGFRRSVWSSRAAVMAAEEKDPLSYFAAYGSSSSGSSDEEDNSEPEETSRRAPDPAKSASGCGNQAEKRLPGPDELFRSVTRPAFLYNPLNKQIDWERHVVKAPEEPPKEFKIWKSNYVPPPETYTTEKKPPPPELDMAIKWSNIYEDNGDDAPQNAKKARLLPEGEETVESDDEKDEHTSKKRKVEPGETAKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MAN3</accession>
    <name>A0A8C4MAN3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Sorting nexin 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SNX7</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Equidae</taxon>
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    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="Wang"/>
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                <person name="Wang"/>
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                <person name="Ju"/>
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                <person name="Li"/>
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                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="5">Heterodimer; heterodimerizes with SNX4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sorting nexin family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A8C4MAN3"/>
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        <property type="gene ID" value="ENSEASG00005015730.2"/>
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    <dbReference type="GeneTree" id="ENSGT00940000155315"/>
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        <property type="component" value="Chromosome 16"/>
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    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:phosphatidylinositol binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07666">
        <property type="entry name" value="BAR_SNX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07284">
        <property type="entry name" value="PX_SNX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042131">
        <property type="entry name" value="BAR_SNX7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042130">
        <property type="entry name" value="PX_SNX7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45949">
        <property type="entry name" value="SORTING NEXIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45949:SF3">
        <property type="entry name" value="SORTING NEXIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00312">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50195">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PX" evidence="7">
        <location>
            <begin position="94"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005023082.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="442" mass="50357" checksum="14F4AE558DE4866A" modified="2023-09-13" version="2">MDGERRASQALSSGLPEGGAGGESPGDGGPVPGSSCSSALLQAEVLDLDEDEDDLEVFSKDASLMDMNSFSPMMPTSPLSMINQVKFEDEPDLKDLFITVDEPESHVTTVETFITYRIITKTSRGEFDSSEFEVRRRYQDFLWLKGKLEDAHPTLIIPPLPEKFIVKGMVERFNDDFIETRRKALQKFLNRIADHPTLTFNEDFKVFLTAQTWELSSHKKQGPGLLSRMGQTVRAVAWSMRGVKSRPEEFMEMNNFIEMFSQKINLIDKISQRIYKEEREYFDEMKEYGPVHILWSATEEDLVDTLKSVASCVDKCCKATEKRMSGLSEALLPVVHEYVLYSEILMGVMKRRDQIQAELDSKVEALTSKKADTDLLTEEIGKLEDKVECANNALKADWERWKQNMQNDIKSAFTDMAEENIHYYEQGARGSCLTWNSQIPVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MAV9</accession>
    <name>A0A8C4MAV9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cyclin-G1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cyclin-G2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CCNG2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">May play a role in growth regulation and in negative regulation of cell cycle progression.</text>
    </comment>
    <comment type="function">
        <text evidence="9">May play a role in growth regulation. Is associated with G2/M phase arrest in response to DNA damage. May be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family. Cyclin G subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MAV9"/>
    <dbReference type="Ensembl" id="ENSEAST00005026235.2">
        <property type="protein sequence ID" value="ENSEASP00005024188.2"/>
        <property type="gene ID" value="ENSEASG00005016433.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156423"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd20584">
        <property type="entry name" value="CYCLIN_CCNG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000056">
        <property type="entry name" value="Cyclin G2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000006">
        <property type="entry name" value="Cyclin I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039361">
        <property type="entry name" value="Cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10177">
        <property type="entry name" value="CYCLINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 12" id="KW-0195">Cyclin</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0498">Mitosis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="14">
        <location>
            <begin position="104"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="343"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00058130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005024188.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="387" mass="43852" checksum="E9BB22B10A12BAE0" modified="2023-09-13" version="2">MPTPRPGVRLGSSSQLSLGSGRQETERRWWAGWRGEEAEDNSLMKDLGAEYLAGREGVQLFGLLSLYLEQEQRFQPREKGLSLIEATPENDNTLCPRLRNAKVEDLRSLTNFFGSCTETFVLAVNILDRFLALMKVKPKHLSCIGVCCFLLAARIVEEECNIPSTHDVIRISQCKCTASDIKRMEKIISEKLHYELEATTALNFLHLYHTIVLCHTSERKEILSLDKLEAQLKACNCRLTFSKARPSVLALCLLNLEVETLKSLELLEILLLVKKHSKVNDAEFIYWRELVSKCLAEYSSPECCKPDLKKLVWIVSRRTAQNLHNSYYSVPELPTIPEGTCFDESESEDSCEDMSCGEESLGSSPLSDQESTFFFNFKVAQTLCFPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MFE9</accession>
    <name>A0A8C4MFE9_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Pappalysin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PAPPA2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M43B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005026971.2">
        <property type="protein sequence ID" value="ENSEASP00005024852.2"/>
        <property type="gene ID" value="ENSEASG00005016901.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158543"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060349">
        <property type="term" value="P:bone morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd04275">
        <property type="entry name" value="ZnMc_pappalysin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000097">
        <property type="entry name" value="Pappalysin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000026">
        <property type="entry name" value="Pappalysin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006558">
        <property type="entry name" value="LamG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011936">
        <property type="entry name" value="Myxo_disulph_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000800">
        <property type="entry name" value="Notch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043543">
        <property type="entry name" value="PAPPA/PAPPA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058897">
        <property type="entry name" value="PAPPA_SD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008754">
        <property type="entry name" value="Peptidase_M43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02232">
        <property type="entry name" value="myxo_disulf_rpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46130">
        <property type="entry name" value="LAMGL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46130:SF1">
        <property type="entry name" value="PAPPALYSIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13385">
        <property type="entry name" value="Laminin_G_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00066">
        <property type="entry name" value="Notch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25900">
        <property type="entry name" value="PAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05572">
        <property type="entry name" value="Peptidase_M43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00560">
        <property type="entry name" value="LamGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00004">
        <property type="entry name" value="NL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0768">Sushi</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040450135" evidence="8">
        <location>
            <begin position="23"/>
            <end position="1790"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="9">
        <location>
            <begin position="1521"/>
            <end position="1590"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="9">
        <location>
            <begin position="1591"/>
            <end position="1646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="81"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="224"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="140"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005024852.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1790" mass="198007" checksum="65EFCFE0FF9EE845" modified="2023-09-13" version="2">MMCLRILRISLVILAAWALGTASSEPGWTRKRSLVQRGHLNQVLSEGERCWLGAKVRRLRAAPQHHLFGVYPSRPGNYLRPYALGDQGNHHAGQSKPAADGVAVSHVPPDPSGSAGVRSEAEQPAAPQAGDGPIGQSKLRGHDDTYLGDPASKESLGEAEIQEGSVMAATITTTFARPKEPEPGTQRKGLAKTRRRRQAVKGQAEGRQRDPDVSPWRFQPWPQHAFVHGDRNSPLEDSIQDGGGDPYGEAETFNPDRGLPVLYFSGRRERLLLRPEVLAEIPREEFTVEVWVKPEGGQNNPAIIAGVFDNCSHTVSDKGWALGIRSGKDMRRRDARFFFSLRTDRMKKATIVIGHSRYQPGTWTHVAATYNGQRMALYVDGTQVASSSDQSGPMNSPFMASCRSLILGGDSSENGHYFRGHLGTLVVWSTALPQSHLQHSPQYPSRVEELTTLILTASFEPLEEQWAPFRDGNYPRLEVLQGFESEPEILSPLQPPLCGQTACDNVELISQYNGHWPLRGEKVIRYQVVNICDDKGLNPTVSEEQIRRQHEALNEAFSRYNISWQLSVHQVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGYDGGDCRLHGRCYSWNRRDGLCHVECNNMLNDFDDGDCCDPEVADVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNVYFASSVREDLAGAATWPWDKEAVSHLGGVVLNPSYYGMSGHTNIMIHEVGHVLGLYHVFKGVSERESCDDPCRETVPSMDTGDLCADTAPTPKSKLCRDPEPTNDTCGFTHFPGAPFSNYMSYTDDDCTDNFTPNQVARMHCYLDLVYQQWSQSQKPTPIPIPPMVIGQTHKSLTIHWLPPISGVVYDRAPGSVCGACTEDGTFRQYVYTASSRRVCDSSGYWTPEEAVGPPDVDQPCEPSLQAWSPELHLYHMNMTVPCPSEGCSLELLFQHPVQADMLTLWVTYLSMDSSQALFDTEILVEHQESVHLGPLDTFCDTPLTIKLHVDGKVLGVKVYTFDERMEVDAALLTSQPHSPLCSGCRPVSYQVLREPPFASGLPVVVTDPHRKFTDVEVTPGQMYRYQVQAEAGAELGEASPPLSHIHGAPYCGDGKVARSLGEECDDGDLLSGDGCSRACELEEGFNCEGEPSLCYIFEGDGICEPFEKETSIIDCGLYTPKGYLDQWAAQAYSSREDKKCPVSLVTGEPHSMICTSYHPDLLEHRPLTGWFPCATRGNRPQDERSEQPEGSLERENEVWLKVCFSRPGVATAIFLFLTSDGLVPGEHQRPTVTLHLTDVSGNNHSLGTYELSCQHNPLVINVTHHPNVLFHHTTSVLLNFSSSLVGISAVALRTSSHISPSATNNCIPEHEGPNHQAQSCVHRPCGEQGSCVPLLLDHADVVNCTSGGPGHMKCAITCQRGFALQASSGQYLRPMQKEILLTCSSGHWDRAVSCMPIDCGVPDPSLVNYATFSCSKGTDFLKRCSITCVPPAKLQGLSPWLTCLEDGLWSLPEAYCKLECDAPPDIRNAKLLLPHCLQGNHDVGSVCRYECKPGYYVVESVESKVRNKFLKIQCLEGGIWEQGSCVPVVCEPPSPVFEGMYECTNGFELDSQCVLKCNQESERLPILCTKEGLWTEEFKLCENLQQGECPPPPSELNLVEYKCEQGYGIGAVCSPLCVIPPSDPVILPENVTADTLEHWMEPVKVQSIMCTGRRQWHPDPFLVHCIQACEPFQADGWCDTINNRAYCHYDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MGM4</accession>
    <name>A0A8C4MGM4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Forkhead box N4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">FOXN4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MGM4"/>
    <dbReference type="Ensembl" id="ENSEAST00005029250.2">
        <property type="protein sequence ID" value="ENSEASP00005026941.2"/>
        <property type="gene ID" value="ENSEASG00005018357.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158984"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035881">
        <property type="term" value="P:amacrine cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010842">
        <property type="term" value="P:retina layer formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060579">
        <property type="term" value="P:ventral spinal cord interneuron fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd20057">
        <property type="entry name" value="FH_FOXN4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000122">
        <property type="entry name" value="Forkhead box protein N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047119">
        <property type="entry name" value="FOXN2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13962">
        <property type="entry name" value="FORKHEAD BOX PROTEIN N3-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13962:SF17">
        <property type="entry name" value="FORKHEAD BOX PROTEIN N4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="9">
        <location>
            <begin position="193"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="7">
        <location>
            <begin position="193"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="119"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="365"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026941.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="517" mass="54828" checksum="CA45C3F9D07AE2F1" modified="2023-09-13" version="2">MIESDISSMMSGIIRNSGQHRHPPPQEYRLLATSSDDDLPGDLQSLSWLTAVDVPRLQQMASGRVDLGGPSAPHPHPGALAGAADLHVGATPGPLLHGPAGMGPQGVLGLGPLASHGANMSQFPVGGQPSPRLQDPPQLYSPASQPQFPLPLGAQQCPPVGLCGPPFGARPSYPQPRGAVHSSQDLHPKHYPKPIYSYSCLIAMALKSSKTGSLPVSEIYSFMKEHFPYFKTAPDGWKNSVRHNLSLNKCFEKVENKMGGSSRKGCLWALNLARIDKMEEEMHKWKRKDLAAIHRSMANPEELDKLISDRPESCRRPGKPGEPEAPVLTHATTVAMAHGCLAVSQLPPQPLMTLSLQSVPLHHQVQPQAHLAPDSPAPAQTPPLHALPDLSPSPCPHPAMGRAPVDFINISTDMSTEVDALDPSIMDFALQGNLWEEMKEEGFSLDALGAFADSPLGCELGASGLTSVSGGSHQSFPDSQGTGLYATYSTPDSVAASAAASPSQYLGAPGNKPIALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MIP7</accession>
    <name>A0A8C4MIP7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine/threonine-protein kinase ULK3</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Unc-51-like kinase 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ULK3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts (via protein kinase domain) with SUFU.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4MIP7"/>
    <dbReference type="Ensembl" id="ENSEAST00005025097.2">
        <property type="protein sequence ID" value="ENSEASP00005023127.2"/>
        <property type="gene ID" value="ENSEASG00005015761.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157689"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072537">
        <property type="term" value="P:fibroblast activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045879">
        <property type="term" value="P:negative regulation of smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045880">
        <property type="term" value="P:positive regulation of smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061709">
        <property type="term" value="P:reticulophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd02684">
        <property type="entry name" value="MIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14121">
        <property type="entry name" value="STKc_ULK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000241">
        <property type="entry name" value="Putative serine/threonine-protein kinase ULK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000238">
        <property type="entry name" value="Putative serine/threonine-protein kinase ULK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000008">
        <property type="entry name" value="serine/threonine-protein kinase ULK3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.80:FF:000010">
        <property type="entry name" value="serine/threonine-protein kinase ULK3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045269">
        <property type="entry name" value="Atg1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036181">
        <property type="entry name" value="MIT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE UNC-51-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24348:SF65">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE ULK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04212">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00745">
        <property type="entry name" value="MIT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116846">
        <property type="entry name" value="MIT domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="105"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="20"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="68"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="28"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00055316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005023127.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="563" mass="62216" checksum="964C313B0A324A93" modified="2023-09-13" version="2">MDLAGTGGTCDFQWGWRPWMRTGAAPGAPGGAGRGAGRGGGGEPAPGSALHFRRAALGAAWGPAGRQLALRNPAPGTRKRLASGAGRGAAGMAGPGWGPPRLDGFILTERLGSGTYATVYKAYAKKDTREVVAIKCVAKKSLNKASVENLLTEIEILKGIQHPHIVQLKDFQWDSDNIYLIMEFCAGGDLSRFIHTRRILPEKVARVFMQQLASALQFLHQQNISHLDLKPQNILLSSLEKPHLKLADFGFAQHMSPWDEKHVLRGSPLYMAPEMVCQRQYDARVDLWSVGVILYEALFGQPPFASRSFSELEEKIRSNRVIELPLRPPLSPDCRDLLQRLLERDPSRRISFQDFFAHPWVDLEHMPSGESLAQATALVVQAVKKDQEGDAAAALSLYCKALDFFVPALHYEVDAQRKEAIKAKVGQYVSRAEELKAIVSSSNRALLRQGASARDLLREMARDKPRLLAALEVASAAMAKEEEAGGEQDALALYQHGLEELLLLLAAEPPGRRRELLHTEVQNLMARAEYLKEQVKMRESRWEAETLDKEGLLESVRSSCTLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MKH0</accession>
    <name>A0A8C4MKH0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Nucleolar GTP-binding protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GTPBP4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Associates with pre-60S ribosomal particles. Interacts with MINAS-60 (product of an alternative open reading frame of RBM10).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
            <location evidence="1 7">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. NOG subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014711743.1">
        <property type="nucleotide sequence ID" value="XM_014856257.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MKH0"/>
    <dbReference type="Ensembl" id="ENSEAST00005030490.2">
        <property type="protein sequence ID" value="ENSEASP00005028074.1"/>
        <property type="gene ID" value="ENSEASG00005019082.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106840620"/>
    <dbReference type="KEGG" id="eai:106840620"/>
    <dbReference type="CTD" id="23560"/>
    <dbReference type="GeneTree" id="ENSGT00390000018475"/>
    <dbReference type="OMA" id="EWKNDVM"/>
    <dbReference type="OrthoDB" id="415015at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0030336">
        <property type="term" value="P:negative regulation of cell migration"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0022408">
        <property type="term" value="P:negative regulation of cell-cell adhesion"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0008156">
        <property type="term" value="P:negative regulation of DNA replication"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010972">
        <property type="term" value="P:negative regulation of G2/M transition of mitotic cell cycle"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031397">
        <property type="term" value="P:negative regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01897">
        <property type="entry name" value="NOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1190:FF:000001">
        <property type="entry name" value="Nucleolar GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000496">
        <property type="entry name" value="Nucleolar GTP-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024926">
        <property type="entry name" value="NOG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010674">
        <property type="entry name" value="NOG1_Rossman_fold_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012973">
        <property type="entry name" value="NOG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45759">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06858">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08155">
        <property type="entry name" value="NOGCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038919">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="OBG-type G" evidence="9">
        <location>
            <begin position="169"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="542"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="466"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="544"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="559"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="573"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="586"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="605"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="619"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR038919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005028074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="634" mass="74060" checksum="26EEF499D8FAD4DB" modified="2022-01-19" version="1">MAHYNFKKITVVPSAKDFIDLTLSKTQRKTPTVIHKHYKIHRIRHFYMRKVKFTQQNYHDRLSQILTDFPKLDDIHPFYADLMNILYDKDHYKLALGQVNIAKNLVDNVAKDYVRLMKYGDSLYRCKQLKRAALGRMCTIIKRQKQSLEYLEQVRQHLSRLPTIDPNTRTLLLCGYPNVGKSSFINKVTRADVDVQPYAFTTKSLFVGHMDYKYLRWQVVDTPGILDHPLEDRNTIEMQAITALAHLRAAVLYVMDLSEQCGHGLKEQLELFQNIRPLFINKPLIVVANKCDVKRIAELPEDDQKIFIDLQAEGFPVVETSTLTEEGVIKVKTEACDRLLAHRVETKMKGNKVNEVLNKLHLAIPNKRDDKERPPFIPEGVVARKKRMEIGEPKRKRERDIELEMGDDYILDLQKYWDIMNSSEKYDKIPEIWEGHNIADYIDPDIMQKLEELEKEEELRTAAGEYDSESESEDEEMLEIRQLARQIREKKKLKILQSKEKNTQGPRMPRTAKKVQRKVLENEMRILGVDMDDKDNAHYAVQARRSRSVTRKRKREDSVPPSSVARSRSCSRTPRDVSGLRDVKMVKKAKTMMKNAQKKMNRLGKKGEADRHVFDMKPKHLLSGKRKAGKKDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MLP4</accession>
    <name>A0A8C4MLP4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Radial spoke head 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">RSPH3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the flagellar radial spoke RSP3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MLP4"/>
    <dbReference type="Ensembl" id="ENSEAST00005030339.2">
        <property type="protein sequence ID" value="ENSEASP00005027935.2"/>
        <property type="gene ID" value="ENSEASG00005018991.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004172"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009290">
        <property type="entry name" value="Radial_spoke_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21648">
        <property type="entry name" value="FLAGELLAR RADIAL SPOKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21648:SF0">
        <property type="entry name" value="RADIAL SPOKE HEAD PROTEIN 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06098">
        <property type="entry name" value="Radial_spoke_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0282">Flagellum</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="338"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="213"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="386"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="407"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005027935.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="420" mass="48933" checksum="E3446BC7C359C6DE" modified="2023-09-13" version="2">VAPTWTDRTSPAMGTYTYTYTSRPRPPPCQRRRYRDSLLQPAEEPIRYGNIMYDRRVIRGNTYALQMAPLSGQPDPLEIQRQHQAKRRALARKRAQEQLRPHTPEPVEGRQHVDVQTELYLEEIADRTIEVDMECQTDAFLDRPPTPLFIPAKTGKDVATQILEGELFDFDLEVKPMLEVLVGKTIEQSLLEVMEEEELANLQASQYAYEELRNVELAEVQRLEEQERRHQEEKERRKQQQWEVVHKHNETAQKIAAQVFAQRYLADLLPSVFDSLRDGGYFYDPVQRDIEIGFLPWLMNEVDKTMEYSMVGRAVLDMLIREVVDKRLMMYEHKEDERLSFRPGDRLSGPGAVRDPLVGPEIQGQSTSQSQGPPPDRESLPPLDGRYLEKLSSQERRLLEEEEDEQREMRKSSGKEEPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MM88</accession>
    <name>A0A8C4MM88_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">T-cell activation Rho GTPase-activating protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">T-cell activation GTPase-activating protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TAGAP</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MM88"/>
    <dbReference type="Ensembl" id="ENSEAST00005030343.2">
        <property type="protein sequence ID" value="ENSEASP00005027938.2"/>
        <property type="gene ID" value="ENSEASG00005018994.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157993"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04402">
        <property type="entry name" value="RhoGAP_ARHGAP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000036">
        <property type="entry name" value="T-cell activation Rho GTPase-activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047886">
        <property type="entry name" value="ARHGAP20-like_RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047887">
        <property type="entry name" value="ARHGAP20_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23179">
        <property type="entry name" value="T-CELL ACTIVATION RHO GTPASE ACTIVATING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23179:SF26">
        <property type="entry name" value="T-CELL ACTIVATION RHO GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22286">
        <property type="entry name" value="RHG20_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rho-GAP" evidence="7">
        <location>
            <begin position="283"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="485"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="564"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="653"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="494"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="581"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="653"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="684"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00074142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00077368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005027938.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="912" mass="101220" checksum="54EA04B934448684" modified="2023-09-13" version="2">MPGPQSLRAPAGRSRHGVPATKGTLRILAQPRSSAPACGLGKILSKAWPPTRKSLASRASLQQPDLPEQQGEPILQSPVVLTQGPETKQRHLFLFRDWLVIAKRRSSKSYGLKQKLPLSDLGVVSCDTGEEQEEDKDGDLITRGGNAIFFIMASDPCVTEFPSPEVKELWLEALRGQSRGHTGAKFITGQSAQSLMKLISSCNVSKTLNANNMETLIECQSEGDIKEHPLLVSCESEESICQLIEIKKTKKVLSWPFLMKRFSPSSDFSGASEPELKVSLFDQPLSIICGDDDTLPRPIQDILTILCVKGPSTEGIFRKGANEKARKELKEELNSGGVVDLQSLPVNLLAAVFKDFLRSIPLKLLSCDLFEEWMGALEKLNEEDRIEALKQVAEKLPQPNLLLLKHLVSVLYLISKNAEVTKMDASNLAICVGPNMLSRENDQNLSLEAQKDLNNKVKTLVEFLIDNCLEIFGENIPVHSSIASDDSLEHTDSSDMSTLQNDSAYDSNDPDVESSSAIDSPSRRPQVPSETAASLEPRGPERTWELSREPIVSTVARLKSSLSQLDRRYSEPNMPSSQECLESRKTTQKLTRSEDDFTMARNSSHFESEEAEDPFPEEVFPAVESKTQRPQDLKVKYWTQGLPSGLVPKAFSSGSLDASSDSSPMASPSSPQRSFFTRHQSFTKTEKSKPNREIKKHSMSFSFASHKKVLSKTSSFGSGKPKGFPRDQARKDLKKESQLAGRIVQEDLSEIHGQTNLDISSRCYALSVENVFQLVDQRCPGSPPSYEESIRCQALELAAYGSQTVGSVRAKMLSQDSGLSALLPFRHGGDSRNICHREPLDGHGTESWKQRRTVCASVETIGQVTVPRRPELPRLRTVSESKQKTKWDYLVRRCSQPIFEADQLQFARESYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MN31</accession>
    <name>A0A8C4MN31_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">RAS guanyl-releasing protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Calcium and DAG-regulated guanine nucleotide exchange factor I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">RASGRP2</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Cell projection</location>
            <location evidence="17">Ruffle membrane</location>
            <topology evidence="17">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Synapse</location>
            <location evidence="16">Synaptosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RASGRP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044618528.1">
        <property type="nucleotide sequence ID" value="XM_044762593.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044618529.1">
        <property type="nucleotide sequence ID" value="XM_044762594.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070344229.1">
        <property type="nucleotide sequence ID" value="XM_070488128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MN31"/>
    <dbReference type="Ensembl" id="ENSEAST00005031714.2">
        <property type="protein sequence ID" value="ENSEASP00005029176.1"/>
        <property type="gene ID" value="ENSEASG00005019867.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106833617"/>
    <dbReference type="CTD" id="10235"/>
    <dbReference type="GeneTree" id="ENSGT00940000160483"/>
    <dbReference type="OMA" id="LHIYYQQ"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000023">
        <property type="entry name" value="RAS guanyl-releasing protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.870.10:FF:000011">
        <property type="entry name" value="RAS guanyl-releasing protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.840.10:FF:000003">
        <property type="entry name" value="Ras guanyl-releasing protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF16">
        <property type="entry name" value="RAS GUANYL-RELEASING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 21" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0770">Synapse</keyword>
    <keyword evidence="6" id="KW-0771">Synaptosome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="25">
        <location>
            <begin position="4"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="23">
        <location>
            <begin position="154"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="26">
        <location>
            <begin position="426"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="24">
        <location>
            <begin position="498"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="382"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="555"/>
            <end position="591"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00046295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00059239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00070997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00083031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005029176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="608" mass="69210" checksum="26D5A00AAB4808E8" modified="2022-01-19" version="1">MAGTLDLDKGCTVEELLRGCIEAFDDCGKVREPELVRMFLMMHSWYIPSSQLAAKLLHIYQQSRKDNSNSLQVKTCHLVRYWISAFPAEFDLNPELAKQIKELKALLDQEGNRRHSSLIDIDSVPTYQWKRQVTQRNPMGQKKRKMSLLFDHLEPMELAGHLTFLEYRSFCKILFQDYHSFVTHGCTVDNPILERFISLFNSVSQWVQLMILSKPTAPQRAAVITHFVHVAEKLLELQNFNTLMAVVGGLSHSSISRLKETHSHVSPETIKLWERLTELVTATGNYGNYRRRLASCRGFCFPILGVHLKDLVALQLALPDWLDPARTRLNGAKMKQLFNILEKLTMVTSVEPPVQANPDLLSLLTVSLDQYQTEDELYQLSLQREPRSKSSPTSPTSCTPPPRPPVLEEWTSAAKPKLDQALLVEHIEKMVESVFRNFDVDGDGHISQEEFQIIRGNFPYLSAFGDLDQNQDGCISREEMVSYFMRSSSVLGGRMGFVHNFHESSSLRPVACRHCKTLILGIYKQGLKCRACGVNCHKQCKDRLSVECRRRAQSVSLEGSAHSPSPTPTHHRAFSFSLPRPGRRGSRPPEIREEEVQTVEDGVFDIHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MT16</accession>
    <name>A0A8C4MT16_EQUAS</name>
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        <submittedName>
            <fullName evidence="4">Retrotransposon Gag like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RTL1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_014710038.2">
        <property type="nucleotide sequence ID" value="XM_014854552.3"/>
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    <dbReference type="GeneTree" id="ENSGT00950000183173"/>
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        <property type="component" value="Chromosome 7"/>
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    </dbReference>
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        <property type="entry name" value="HIV Type 1 Reverse Transcriptase, subunit A, domain 1"/>
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    <dbReference type="InterPro" id="IPR032567">
        <property type="entry name" value="RTL1-rel"/>
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    <dbReference type="PANTHER" id="PTHR15503">
        <property type="entry name" value="LDOC1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15503:SF39">
        <property type="entry name" value="RETROTRANSPOSON-LIKE PROTEIN 1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="184"/>
            <end position="267"/>
        </location>
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    <feature type="domain" description="Reverse transcriptase/retrotransposon-derived protein RNase H-like" evidence="3">
        <location>
            <begin position="832"/>
            <end position="928"/>
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            <begin position="1"/>
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            <begin position="1326"/>
            <end position="1349"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="31"/>
            <end position="40"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="89"/>
            <end position="99"/>
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            <begin position="570"/>
            <end position="579"/>
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            <begin position="1326"/>
            <end position="1335"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1336"/>
            <end position="1349"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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    <evidence type="ECO:0000259" key="2">
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            <dbReference type="Pfam" id="PF16297"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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    <sequence length="1349" mass="153914" checksum="EE2EF2374F1D03E8" modified="2023-09-13" version="2">MIEPSEDSFETMMERKNPSSKQMESSEGSSNTTVETSGSGEQEAAGLASGPAQEMKELPIDLLQDMEEPSSGPHREIKDPPNDLLQDLEESRIGSHQEVGDPFSEAPGRMEEASVNPWGAQEGQGNNTNLAKSGEVTGPEEQTEISTAETMAKVRSIISLYFRMQDLREQQRVAEEILMRGISAGQLPLPKQFSGDRREYREFIVLCQLILQSCPRMFYSDRLRVGYVICHLSGLALEWANALVEEDSPLLNNFPAFLEAMSDMFEYRQALRVAEEAMFNIRQGDRPAIEYINEFQGLVPTLGWPDEVLQAHLCHGLNEEIRHHLFRVPQPDSLQNLIVLVLQIEDKLAERRAMLRLPPEARPRNLTWIDSPAPERWKVSSWLPCEVHPTIDRDHLFLLLMVRVNPYHSVAVQALVDSGAGANFMDERFAQEHYVELYEKPYPQSIQTRDGSLIGDHPVWLYTEPLVCIHQNHQETLEFDIVPSPNFSVILGTKWLQVHAPEVDWIKGRCTFHSPYCLKNCLRPPQPCIALERHAIRLLPGLPPLYSDLADVFNPKEADDETSDQPSSDGSDDLSESEPSELQQAGDSDHSETFYECPSTAPWEPVGARMQERARLRDEFWDLQDMLTNRQDYIQMIPELFDQLHGATWFTKLELRGTIVEESMNVCQAEDVWRAAFGLELQEMESYKPFQLSSDPVIPQKVIHFILKDMLGYFVLSYGQDVLIYSMSQEEHLQHVRHVLVRFRHHKVYCSLDRSQFHRHTVEFLGFVMTPKGVKLNKSIVTTVTGYPVPGSKKSLRHLIEFTFPYRHFVERFTIITEALVRQLLTSEPFFWGDEEQEAFECLKRAFRQAPLLHHPKPQNPFYLETGVTKTALHASLIQIDNQTGKRVSCAFYSRNISPIEVDYSQLEMKILPIRAAFMVWCRYLENTEEPIMILLNTEDLASLNNDRLTVLLPGHWVFFFSHFNFDVMERPEQDGGRSLPPVRNLNQRALQHNTATRSLMLLATRRSLRDQLPESREEENGDAPHQDETNGQTLQQEFLALIPTDQILSSLLAHFSIAQIRAVILHFFRGLLFWKNILAVAALLALLKLRQPLALLPAPTLEVAQPRPRRSLRLILDTSLLTGSGIAPAITQLLAQMPPLMGTNTIPAEELAELFLRPGHRQQTALVPQTRRGPPFMPRLWLTLREFFGVRVALQEGTSPLLLENRYLELHVVGDEDVVLREALQDDLQRYRQCGLHDGLQDTSQDEQDDVQEALATDTTLALRPHQRVPGDSQVLAILNNRQLYVRSTHRQPSVASPGPMARALTHFLTGIYAQAPLPIIRVTPPREEARLEEIPEEDEEEEDSDLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MW82</accession>
    <name>A0A8C4MW82_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Pyruvate kinase</fullName>
            <ecNumber evidence="18">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">PKLR</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Pyruvate kinase that catalyzes the conversion of phosphoenolpyruvate to pyruvate with the synthesis of ATP, and which plays a key role in glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:18159"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 18">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 18">Belongs to the pyruvate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4MW82"/>
    <dbReference type="Ensembl" id="ENSEAST00005030765.2">
        <property type="protein sequence ID" value="ENSEASP00005028332.2"/>
        <property type="gene ID" value="ENSEASG00005019252.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008859"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00288">
        <property type="entry name" value="Pyruvate_Kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.33.10:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.60:FF:000025">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1380.20:FF:000001">
        <property type="entry name" value="Pyruvate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018209">
        <property type="entry name" value="Pyrv_Knase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004491">
        <property type="entry name" value="PRK05826.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004978">
        <property type="entry name" value="PRK06354.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00110">
        <property type="entry name" value="PYRUVATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14 18" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 18" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0630">Potassium</keyword>
    <keyword evidence="15" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="19">
        <location>
            <begin position="85"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="20">
        <location>
            <begin position="453"/>
            <end position="570"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00058419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005028332.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="573" mass="61939" checksum="BCE4C72DDA0F50FC" modified="2023-09-13" version="2">MSTKGSKRLQQSWSRISKSQRDLAKSILTGAPGGQGYLRRASVAQLTQELGTAFFQRQQLPAAMADTFLEHLCLLDIDSEPLAARSTSIIATIGPASRSVELIKEMIKAGMNIARFNFSHGSHEYHAESIANVRKAAESFATSPLSYRPVAIALDTRGPEIRTGILQGGLESKVELVKGSQVLVTVDPAFQIQGNANIVWVDYPNIVRVMPLGSHIYIDDGLISLRVKKIGPKGLETEVESGGLLGSRKGVNLPGIQVDLPGLSEQDVQDLRFGVEHGVDIVFASFMRKASDVVAVRAALGPKGQGIKIISKIENHEGVKKFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMIIGRCNLAGKPVVCATQMLESMITKSRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVVAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQVARQAHLCRGVFPLLYHEPPEAIWADDVDRRVQFGIESGKLRGFLHVGDLVIVVTGWRPGSGYTNIMRVLSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MWV7</accession>
    <name>A0A8C4MWV7_EQUAS</name>
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            <fullName evidence="11">Exosome complex component RRP43</fullName>
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        <alternativeName>
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        <alternativeName>
            <fullName evidence="10">Ribosomal RNA-processing protein 43</fullName>
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    <gene>
        <name evidence="15" type="primary">EXOSC8</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MWV7"/>
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        <property type="protein sequence ID" value="ENSEASP00005029127.2"/>
        <property type="gene ID" value="ENSEASG00005019832.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183130"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0035925">
        <property type="term" value="F:mRNA 3'-UTR AU-rich region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000467">
        <property type="term" value="P:exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071035">
        <property type="term" value="P:nuclear polyadenylation-dependent rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071038">
        <property type="term" value="P:TRAMP-dependent tRNA surveillance pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034473">
        <property type="term" value="P:U1 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034476">
        <property type="term" value="P:U5 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11369">
        <property type="entry name" value="RNase_PH_RRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.70:FF:000011">
        <property type="entry name" value="exosome complex component RRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050590">
        <property type="entry name" value="Exosome_comp_Rrp42_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033196">
        <property type="entry name" value="Rrp43"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097:SF9">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11097">
        <property type="entry name" value="EXOSOME COMPLEX EXONUCLEASE RIBOSOMAL RNA PROCESSING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0271">Exosome</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="13">
        <location>
            <begin position="25"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="14">
        <location>
            <begin position="187"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
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            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005029127.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="270" mass="29359" checksum="45E9A2883A95E10B" modified="2023-09-13" version="2">TVEPLEYYRRFLKENCRPDGRELGEFRTTTVNIGSISTADGSALVKLGNTTVICGIKAEFAAPPTDAPDKGYVVPNVDLPPLCSSRFRSGPPGEEAQVASQFIADVIENSQIIQKEDLCISPGKLAWVLYCDLICLDYDGNILDACTFALLAALKNVQLPEVTISEETALAEVNLKKKSFLNIRTHPVATSFAVFDDTLLIVDPTGEEEHLATGTLTVVMDEEGKLCCLHKPGGSGLTGAKLQDCMSRAVTRHKEVKKLMDEVVKSMKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N1J0</accession>
    <name>A0A8C4N1J0_EQUAS</name>
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        <recommendedName>
            <fullName evidence="9">Cleavage stimulation factor subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cleavage stimulation factor 50 kDa subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CSTF1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). Interacts (via repeats WD) directly with CSTF3. Interacts (via repeat WD6) with BARD1. Interacts with ERCC6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014687594.1">
        <property type="nucleotide sequence ID" value="XM_014832108.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014687598.1">
        <property type="nucleotide sequence ID" value="XM_014832112.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014687606.1">
        <property type="nucleotide sequence ID" value="XM_014832120.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070342260.1">
        <property type="nucleotide sequence ID" value="XM_070486159.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4N1J0"/>
    <dbReference type="SMR" id="A0A8C4N1J0"/>
    <dbReference type="Ensembl" id="ENSEAST00005037108.2">
        <property type="protein sequence ID" value="ENSEASP00005034039.1"/>
        <property type="gene ID" value="ENSEASG00005023272.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106825378"/>
    <dbReference type="KEGG" id="eai:106825378"/>
    <dbReference type="CTD" id="1477"/>
    <dbReference type="GeneTree" id="ENSGT00910000144253"/>
    <dbReference type="OMA" id="HTEDYVM"/>
    <dbReference type="OrthoDB" id="14421at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005848">
        <property type="term" value="C:mRNA cleavage stimulating factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.960.50:FF:000001">
        <property type="entry name" value="Cleavage stimulation factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000064">
        <property type="entry name" value="Cleavage stimulation factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000089">
        <property type="entry name" value="Cleavage stimulation factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.960.50">
        <property type="entry name" value="Cleavage stimulation factor subunit 1, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044633">
        <property type="entry name" value="CstF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032028">
        <property type="entry name" value="CSTF1_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038184">
        <property type="entry name" value="CSTF1_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44133">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44133:SF2">
        <property type="entry name" value="CLEAVAGE STIMULATION FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16699">
        <property type="entry name" value="CSTF1_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Cleavage stimulation factor subunit 1 dimerisation" evidence="11">
        <location>
            <begin position="8"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="104"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="169"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="258"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="309"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005034039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="431" mass="48396" checksum="900EEFBF14588915" modified="2022-01-19" version="1">MYRTKVGLRDRQQLYKLIISQLLYDGYISIANGLINEIKPQSVCAPSEQLLHLIKLGMENDDTAVQYAIGRSDTVAPGTGIDLEFDADVQTMSPEASEYETCYVTSHKGPCRVATYSRDGQLIATGSADASIKILDTERMLAKSAMPIEVMMNETAQQNMENHPVIRTLYDHVDEVTCLAFHPTEQILASGSRDYTLKLFDYSKPSAKRAFKYIQEAEMLRSISFHPSGDFILVGTQHPTLRLYDINTFQCFVSCNPQDQHTDAICSVNYNPSANMYVTGSKDGCIKLWDGVSNRCITTFEKAHDGAEVCSAIFSKNSKYILSSGKDSVAKLWEISTGRTLVRYTGAGLSGRQVHRTQAVFNHTEDYVLLPDERTISLCCWDSRTAERRNLLSLGHNNIVRCIVHSPTNPGFMTCSDDFRARFWYRRSTTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PGJ4</accession>
    <name>A0A8C4PGJ4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">medium-chain acyl-CoA ligase</fullName>
            <ecNumber evidence="14">6.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LOC106839008</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a medium-chain fatty acid + ATP + CoA = a medium-chain fatty acyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:48340"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:59558"/>
            <dbReference type="ChEBI" id="CHEBI:90546"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:48341"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.2" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4PGJ4"/>
    <dbReference type="Ensembl" id="ENSEAST00005004243.2">
        <property type="protein sequence ID" value="ENSEASP00005003863.2"/>
        <property type="gene ID" value="ENSEASG00005002924.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161138"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004321">
        <property type="term" value="F:fatty-acyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000007">
        <property type="entry name" value="Acyl-coenzyme A synthetase ACSM2A, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000005">
        <property type="entry name" value="Acyl-coenzyme A synthetase ACSM5, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051087">
        <property type="entry name" value="Mitochondrial_ACSM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605">
        <property type="entry name" value="ACYL-COENZYME A SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43605:SF5">
        <property type="entry name" value="ACYL-COENZYME A SYNTHETASE ACSM1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="16">
        <location>
            <begin position="72"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="17">
        <location>
            <begin position="483"/>
            <end position="563"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003863.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="577" mass="64742" checksum="18ED70DE6B93E852" modified="2023-09-13" version="2">MQWLMKFRVLWGIHKSCHGFLPAPRHLRCQFLSGAEAPRWNDHDTPEEFNFASGVLDYWTQMEKEGKRGPNPALWWVNDQGDEVKWSFREMTDLTRRAANVFTQTGGLRQGDHLALILPRVPEWWLVTVGCIRTGIVFTPGTTLMKAKDILYRLQVSKAKGIITTDTLAPEVDSVASECPALKIKFLVSDHSREGWLDFRSLIKSASSDHTCVKSKTMDPMAIFFTSGTSGLPKMAKHNQGLALRSSFPSCRKLLQLKTSDVFWCLSDPGWILAVLASMFEAWTAGSTVFIHHLPRFDPNVIVQTLSKYPITQCLAAPSVFRMILQQDFTSLRFPTLEHTCAGGEALLPEEQEQWRRQTGVLLYQVYGQSETGITCGILRGMKIKPGSMGKAIPPFDVQIIDDKGNVLPPNTEGNIGIRIKPTKPIGLFMCYQDDPVKTAETECGDFYNTGDRATMDAEGYIWFLGRSDDVINASGYRIGPTEVENALAEHPAVAESAVVSSPDPIRGEVVKAFVVLTPQFLSRDRDQLTKELQEHVKSVTAPYKYPRKVEFVPELSKTVTGKIKRNELRKKEFGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PIY8</accession>
    <name>A0A8C4PIY8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Ral guanine nucleotide dissociation stimulator like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">RGL1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_014850296.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044613600.1">
        <property type="nucleotide sequence ID" value="XM_044757665.2"/>
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    <dbReference type="AlphaFoldDB" id="A0A8C4PIY8"/>
    <dbReference type="SMR" id="A0A8C4PIY8"/>
    <dbReference type="Ensembl" id="ENSEAST00005009898.2">
        <property type="protein sequence ID" value="ENSEASP00005009090.2"/>
        <property type="gene ID" value="ENSEASG00005006506.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106836997"/>
    <dbReference type="KEGG" id="eai:106836997"/>
    <dbReference type="CTD" id="23179"/>
    <dbReference type="GeneTree" id="ENSGT00940000156012"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="RA_RGL"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
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        <property type="entry name" value="Ral guanine nucleotide dissociation stimulator isoform 1"/>
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    <dbReference type="FunFam" id="1.20.870.10:FF:000003">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
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    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030748">
        <property type="entry name" value="RGL1_RA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF199">
        <property type="entry name" value="RAL GUANINE NUCLEOTIDE DISSOCIATION STIMULATOR-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
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    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
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    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="7">
        <location>
            <begin position="100"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="5">
        <location>
            <begin position="267"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="6">
        <location>
            <begin position="683"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="567"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="576"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="621"/>
            <end position="656"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
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    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009090.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="803" mass="90439" checksum="C1D5AC9EA02DBB6D" modified="2023-09-13" version="2">MEGKPVGEPTQVVSKVKLSSKVESTGHWLVEDHARIWEVLKTDESSIQDWGEEVEEGAVYHVTLKRVQIQQAANKGARWLGVEGDQLPPGHTVSQYETCKIRTIKAGTLEKLVENLLTAFGDNDFTYISIFLSTYRGFASTKEVLELLLDRYGNLTSPNCEEDGRQSSSESKTVIRNAIASILRAWLDQCAEDFREPPHFPCLQKLLDYLKRMMPGSDPERRAQNLLEQFHKQEVETDNGFPNTMSFSLEEEEELEGGGSAEFTCFSEDLVAEQLTYMDAQLFKKVVPHHCLGCIWSRRDKKENKHLAPTIRATISQFNALTKCVVSTILGGKELKTQQRAKIIEKWINIAHECRILKNFSSLRAIVSALQSNSIYRLKKTWAAVPKDRMLMFEELSDIFSDHNNHLTSRELLMKEGTSKFANLDSSVKENQKRTQRRLQLQKDMGVMQGTVPYLGTFLTDLTMLDTALQDYIEGGLINFEKRRREFEVIAQIKLLQSACNSYCMTPDQKFIQWFQRQQLLTEEESYALSCEIEAAADASATSPKPRKSMVKRLSLLFLGSDIITSSTPTKEQPKSTASGSSGESMDSVSVSSCESNHSEAEEGSLTPMDTPDDPQKKLSESSSSCSSIHSMDTTSSGMSSLINPLSSPPSCNNNPKIHKRSVSVTSITSTVLPPVYNQQNEDTCIIRISVEDNNGNMYKSIMLTSQDKTPAVIQRAMLKHNLESDPAEEYELVQVISEDKELVIPDSANVFYAMNSQVNFDFILRKKNSMEEQVKLRSRTSLTLPRTAKRGCWSNRHSKITL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PJA4</accession>
    <name>A0A8C4PJA4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Laminin subunit gamma-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Epiligrin subunit gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Kalinin subunit gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Laminin-5 subunit gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Nicein subunit gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">LAMC2</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
            <location evidence="1">Basement membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="nucleotide sequence ID" value="XM_014850287.3"/>
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    <dbReference type="Ensembl" id="ENSEAST00005009999.2">
        <property type="protein sequence ID" value="ENSEASP00005009189.2"/>
        <property type="gene ID" value="ENSEASG00005006555.2"/>
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    <dbReference type="KEGG" id="eai:106836991"/>
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    <dbReference type="GeneTree" id="ENSGT00940000160470"/>
    <dbReference type="OrthoDB" id="430826at2759"/>
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        <property type="component" value="Chromosome 25"/>
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    <dbReference type="GO" id="GO:0005604">
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        <property type="term" value="P:axon guidance"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:positive regulation of cell migration"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
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    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000067">
        <property type="entry name" value="Laminin subunit gamma 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000399">
        <property type="entry name" value="Laminin subunit gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000441">
        <property type="entry name" value="Laminin subunit gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000533">
        <property type="entry name" value="Laminin subunit gamma 2"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000174">
        <property type="entry name" value="Laminin subunit gamma-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
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        <property type="entry name" value="Laminin/Netrin_ECM"/>
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    <dbReference type="InterPro" id="IPR000034">
        <property type="entry name" value="Laminin_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF313">
        <property type="entry name" value="LAMININ SUBUNIT GAMMA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
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</entry>
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                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
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                <person name="S."/>
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                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="80" mass="9386" checksum="2A1D0A8599E88370" modified="2023-09-13" version="2">MFIAALFTIAKTWNQPTCPETDDWIKKMWYIYTMEYYSAIKKDKIGPFTTTWMDLEGIMLSEISQSEKDELYMTPLIGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PN42</accession>
    <name>A0A8C4PN42_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cancer antigen 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">CAGE1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PN42"/>
    <dbReference type="Ensembl" id="ENSEAST00005018897.2">
        <property type="protein sequence ID" value="ENSEASP00005017397.2"/>
        <property type="gene ID" value="ENSEASG00005012039.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001805"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052686">
        <property type="entry name" value="CAGE1_homolog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029381">
        <property type="entry name" value="CAGE1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36864">
        <property type="entry name" value="CANCER-ASSOCIATED GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36864:SF1">
        <property type="entry name" value="CANCER-ASSOCIATED GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15066">
        <property type="entry name" value="CAGE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cancer-associated gene protein 1 N-terminal" evidence="3">
        <location>
            <begin position="1"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="270"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="815"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="334"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="534"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="732"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="270"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="816"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="841"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005017397.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="850" mass="97695" checksum="B332FD9CB65166EC" modified="2023-09-13" version="2">MSEADAMNFSSFSQGFAHSSSPFFMETSSTTSDWPQNEIKNVKRENESKFTLSEEIYSTLDDLLGDVHIGNYSQNVPTQPIDTNISSFRQFEPTCKLHQTEAFNNEMITFQNLTEGLPYTEKPELQSQVYNYAKDTNIKEGPFKEENPKGTSTSTNKEQLAHECVRQPPRRPPPIHCNGETLKFTEMSLAESTPAESALNTGQPQSFLCKENVHSDVEKPFYKENNFNLLDLRASYKTEELAFSSKGMQNSGAIPKTSASHQQAVTVEGVDSPGVASPGSPAGASSSDGASQEDCRTPDTEQSLESLQPLEEDLALNEVLRKLKHTNKKQQTLIQDLQCSKMYLERKVEELQMNTSKQQVFVDIINKLKEKVEELIEEKYRVMLEKNDMDKTLQNLHEILANTQTLLQESRNEKETLQLELKKMKGNYVRLQERYMTEMQQKNKTLSQCIEMDKTLSQKAEEVERLQLLKGELEKATTCALDLLKREKVAREQEFLSLHEEFQKHEKKNLEERQKLKSSLEKLVVQVKNLQFISESEKAENIKLQQQIDEVKNENAKLQHQVARTEEQSYPKSAIAQLKEHLGDVMESDITKDAKIIHSNLVLNRSPCEDESLNPPDVKITSQLASKIRNLLALVVGLLTCQDITNPDAEHFKESEKVSDIMLEKLKSFHLKKKNLDIQLLKHKDRITTLRELIANEKAFQDQVIEVTDFDSNEAKKVRDVPLLLGAKLDKYHNLNEELDFLIAKLGNLLESKEDHCNRLIEENDKYQRHVGNLLNKVISYEEIIECADQRLEISHSQIAHLEERNKHLEDLVRRPREKARKPRLRRLENHPKSMTVMPEILERNRNDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PN81</accession>
    <name>A0A8C4PN81_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Cytosolic carboxypeptidase-like protein 5</fullName>
            <ecNumber evidence="19">3.4.17.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">ATP/GTP-binding protein-like 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Protein deglutamylase CCP5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">AGBL5</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Metallocarboxypeptidase that mediates deglutamylation of tubulin and non-tubulin target proteins. Catalyzes the removal of polyglutamate side chains present on the gamma-carboxyl group of glutamate residues within the C-terminal tail of alpha- and beta-tubulin. Cleaves alpha- and gamma-linked polyglutamate tubulin side-chain, as well as the branching point glutamate. Also catalyzes the removal of alpha-linked glutamate residues from the carboxy-terminus of alpha-tubulin. Mediates deglutamylation of nucleotidyltransferase CGAS, leading to CGAS antiviral defense response activation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>(L-glutamyl)(n+1)-gamma-L-glutamyl-L-glutamyl-[protein] + H2O = (L-glutamyl)(n)-gamma-L-glutamyl-L-glutamyl-[protein] + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:60004"/>
            <dbReference type="Rhea" id="RHEA-COMP:15519"/>
            <dbReference type="Rhea" id="RHEA-COMP:15675"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:143623"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:60005"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>C-terminal L-alpha-aminoacyl-L-glutamyl-L-glutamyl-[tubulin] + H2O = C-terminal L-alpha-aminoacyl-L-glutamyl-[tubulin] + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:63792"/>
            <dbReference type="Rhea" id="RHEA-COMP:16435"/>
            <dbReference type="Rhea" id="RHEA-COMP:16436"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:149555"/>
            <dbReference type="ChEBI" id="CHEBI:149556"/>
            <dbReference type="EC" id="3.4.17.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:63793"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>C-terminal L-alpha-aminoacyl-L-glutamyl-[tubulin] + H2O = C-terminal L-alpha-aminoacyl-[tubulin] + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:63796"/>
            <dbReference type="Rhea" id="RHEA-COMP:16436"/>
            <dbReference type="Rhea" id="RHEA-COMP:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:90782"/>
            <dbReference type="ChEBI" id="CHEBI:149556"/>
            <dbReference type="EC" id="3.4.17.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:63797"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>gamma-L-glutamyl-L-glutamyl-[protein] + H2O = L-glutamyl-[protein] + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:60152"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15517"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:143622"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:60153"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Midbody</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 25">Belongs to the peptidase M14 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.17.24" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4PN81"/>
    <dbReference type="Ensembl" id="ENSEAST00005019846.2">
        <property type="protein sequence ID" value="ENSEASP00005018282.2"/>
        <property type="gene ID" value="ENSEASG00005012611.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158032"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06236">
        <property type="entry name" value="M14_AGBL5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000049">
        <property type="entry name" value="cytosolic carboxypeptidase-like protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000055">
        <property type="entry name" value="Cytosolic carboxypeptidase-like protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.3120:FF:000002">
        <property type="entry name" value="cytosolic carboxypeptidase-like protein 5 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050821">
        <property type="entry name" value="Cytosolic_carboxypeptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034286">
        <property type="entry name" value="M14_AGBL5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040626">
        <property type="entry name" value="Pepdidase_M14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000834">
        <property type="entry name" value="Peptidase_M14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12756:SF12">
        <property type="entry name" value="CYTOSOLIC CARBOXYPEPTIDASE-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18027">
        <property type="entry name" value="Pepdidase_M14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00246">
        <property type="entry name" value="Peptidase_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52035">
        <property type="entry name" value="PEPTIDASE_M14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M14" evidence="27">
        <location>
            <begin position="229"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="36"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="448"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="675"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="855"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="448"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="26">
        <location>
            <begin position="692"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="713"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="26">
        <location>
            <begin position="786"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="25">
        <location>
            <position position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004214"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005988"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00024141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00024627"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00026108"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00029302"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032753"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00032928"/>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00045278"/>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00047714"/>
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    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS52035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005018282.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="958" mass="105199" checksum="4A92DD6883FF0773" modified="2023-09-13" version="2">MNVLHSGRAVGFHWRPGETEGKCGAEIAKAVNRLPRRHGRTPPAVPASRARERPWLSSQGQSGAGGCFPSPTMELRCGGLLFSSRFDSGNLAHVEKVESVSNDGEGVAGGASAPTSSIASSPDYEFNVWTRPDCAETEFENGNRSWFYFSVRGGTPGKLIKINIMNMNKQSKLYSQGMAPFVRTLPTRPRWERVRDRPTFEITETQFVLSFVHRFVEGRGATTFFAFCYPFSYHDCQDLLNQLDQRFLENHPTHSSPRDTIYYHREILCYSLDGLRVDLLTISSCHGLREDREPRLEQLFPDTGTPRPFCFTGKRIFFLSSRVHPGETPSSFVFNGFLDFILRPDDPRAQTLRRLFVFKLIPMLNPDGVVRGHYRTDSRGVNLNRQYLKPDAVLHPAIYGAKAVLLYHHVHSGLKSQSPSEHHYSPRLPADAPLSDFEKANNLQNEAHLGHTSDKDNPKAWTQTEPAEQKPNRVWIMPQQSPEVEQPAPDTIPPKESGVAYYVDLHGHASKRGCFMYGNSFSDESTQVENMLYPKLISLNSAHFDFQGCNFSEKNMYARDRRDGQSKEGSGRVAIYKASGIIHSYTLECNYNTGRSVNSIPAACHDNGRASPPPPPAFPSRYTVEMFEQVGRAMAIAALDMAECNPWPRIVLSEHNSLTNLRAWMLKHVRSSRGLSSTVNVGVNKKRGSRTPPKSNNGLPVSCSENTLSRARSFSTGTSAGGSSSSQQNSPQMKNSPSFPFHGSRPAGLPGLGSSTQKVSHRVLGPVREPRSQDRRRRQQPLTHRSTSSSLAPSPTPASSSPAASRNMGSCLLPSSLSISGSSCSLLSSGDKPEAVMVIGKGLLGTGPRIPCIRTQLQARPRLGRGSPPTCRGMKGSSGPTSPIPRTRESSEPEPGPRSAPGLPQAGPPRSHSAPAFSPISCSLSDSQSWICYSGGPLSQPEVCFVPKSPPLTVSPRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PPF7</accession>
    <name>A0A8C4PPF7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="17">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC106846989</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 17">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Membrane</location>
            <topology evidence="17">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="17"/>
    <dbReference type="RefSeq" id="XP_044635207.1">
        <property type="nucleotide sequence ID" value="XM_044779272.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PPF7"/>
    <dbReference type="Ensembl" id="ENSEAST00005021992.2">
        <property type="protein sequence ID" value="ENSEASP00005020254.2"/>
        <property type="gene ID" value="ENSEASG00005013899.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106846989"/>
    <dbReference type="GeneTree" id="ENSGT00940000161917"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140345">
        <property type="term" value="F:phosphatidylcholine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090555">
        <property type="term" value="F:phosphatidylethanolamine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000025">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000188">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000014">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF52">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE FETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 16 17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 17" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="344"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="898"/>
            <end position="919"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="931"/>
            <end position="951"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="984"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1017"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1049"/>
            <end position="1066"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1093"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="18">
        <location>
            <begin position="42"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="19">
        <location>
            <begin position="869"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="14">
        <location>
            <position position="418"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
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        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
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            <position position="419"/>
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            <name>ATP</name>
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        <ligand>
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            <name>ATP</name>
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        <location>
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            <name>ATP</name>
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        <location>
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            <dbReference type="ARBA" id="ARBA00001946"/>
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            <dbReference type="ARBA" id="ARBA00004127"/>
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    <sequence length="1186" mass="136212" checksum="5F6CC8AD4A08F391" modified="2023-09-13" version="2">MKYIKPFISEIPWKYSWYYSAMSLFESQSENRQSGEPERCLQANNREFNTLFGYPNNTIKTSKYNVLNFLPLNLFEQFQRLANAYFLILLFLQLIPQISSLAWYTTVIPLMVVLSITAVKDAIDDLKRHQNDNQVNNRSVLVLTNGRMKEDKWMNIQVGDIVKVENNQSVTADMLLLSSSEPYSLTYIETAELDGETNLKVKQAISVTSDMEDNLKLLSAFDGEVRCESPNNKLDKFAGILTYKGKNYILDHDKLLLRGCVIRNTDWCYGLVIYTGPDTKLMQNCGKSTFKRTHIDHLMNVLVLWIFLFLGSMCFILAVGHGIWENKKGYYFQNFLPWKEYVSSSVVSAILMFWSYFIILNTVVPISLYVSVEIIRLGNSYYINWDRKMFYAPKNTPAQARTTTLNEELGQVKYVFSDKTGTLTQNIMIFKKCSINGTLYGDVYDKNGQRVEVSEKTEKVDFSYNKLADPKFSFYDKTLVEAVKRGDGRVHLFFLSLSLCHTVMSEEKVEGELVYQAQSPDEGALVTAARNFGFAFRSRTSETITVVEMGETKVYQLLAILDFDNVRKRMSVIVRTPEDRVMLFCKGADTIVCQLLHPSCRSLGDVTMEHLDDFAIEGLRTLMVAYRELDNAFFQAWSKKHSEACLSLEDRENKISNVSEEIEKDLMLLGATAIEDKLQDGVLETIITLNKAKIKMWTLTGDKQETAVNIAYACNIFEDEMDEIFIVEGKDDETIWQELRSARAKMKPESLLESDPVNSYLTMKPKMPFKIPEEVPNGNYGLVINGYSLAYALEGNLELELLRTACMCKAVICCRMTPLQKAQVVELVKRYKKVVTLAIGDGANDVSMIKAAHIGIGISGQEGMQAMLNSDYAFCQFHYLRRLLLVHGRWSYNRMCKFLSYFFYKNFVFTLVHFWYAFFSGFSAQTVYDTWFITFYNLVYTSLPVLGLSLFDQDVNETWSLRFPELYEPGQHNLYFNKKEFVKCLVHGIYSSFVLFFVPMGTVYNSVRSDGKEISDYQSFSLIVQTSLLWVVTMQIALETTYWTMISHFFIWGSLGFYFCVLFFLYSDGLCLMFPNVFQFLGVARNILKLPQLWLSLVLSVVLCMLPVIGYQFLKPLFWPVSVDKVIDRIHHCMRHPLPPPIRTKVKHASSRRSAYAFSHKQGFGALITSGKTMKGKPKKSSLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PQM5</accession>
    <name>A0A8C4PQM5_EQUAS</name>
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        <recommendedName>
            <fullName evidence="5">Coagulation factor IX</fullName>
            <ecNumber evidence="4">3.4.21.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Christmas factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">F9</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.</text>
            <dbReference type="EC" id="3.4.21.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="25">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.22" evidence="4"/>
    <dbReference type="RefSeq" id="XP_014710757.1">
        <property type="nucleotide sequence ID" value="XM_014855271.3"/>
    </dbReference>
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        <property type="protein sequence ID" value="ENSEASP00005023478.1"/>
        <property type="gene ID" value="ENSEASG00005015984.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106840103"/>
    <dbReference type="KEGG" id="eai:106840103"/>
    <dbReference type="CTD" id="2158"/>
    <dbReference type="GeneTree" id="ENSGT00940000159516"/>
    <dbReference type="OMA" id="SYECWCR"/>
    <dbReference type="OrthoDB" id="8909918at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000259">
        <property type="entry name" value="Coagulation factor VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000013">
        <property type="entry name" value="Coagulation factor X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000162">
        <property type="entry name" value="Coagulation factor X (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.740.10:FF:000001">
        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.740.10">
        <property type="entry name" value="Coagulation Factor IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017857">
        <property type="entry name" value="Coagulation_fac-like_Gla_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035972">
        <property type="entry name" value="GLA-like_dom_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000294">
        <property type="entry name" value="GLA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012224">
        <property type="entry name" value="Pept_S1A_FX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050442">
        <property type="entry name" value="Peptidase_S1_coag_factors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278">
        <property type="entry name" value="COAGULATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24278:SF31">
        <property type="entry name" value="COAGULATION FACTOR IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00594">
        <property type="entry name" value="Gla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001143">
        <property type="entry name" value="Factor_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00010">
        <property type="entry name" value="EGFBLOOD"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00001">
        <property type="entry name" value="GLABLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00069">
        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57630">
        <property type="entry name" value="GLA-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00011">
        <property type="entry name" value="GLA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50998">
        <property type="entry name" value="GLA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="18" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="16" id="KW-0106">Calcium</keyword>
    <keyword evidence="20 25" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 25" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0301">Gamma-carboxyglutamic acid</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="14 26" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="22" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11 26" id="KW-0645">Protease</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0964">Secreted</keyword>
    <keyword evidence="15 26" id="KW-0720">Serine protease</keyword>
    <keyword evidence="10" id="KW-0765">Sulfation</keyword>
    <keyword evidence="19" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Gla" evidence="29">
        <location>
            <begin position="47"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="27">
        <location>
            <begin position="93"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="28">
        <location>
            <begin position="224"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="24">
        <location>
            <position position="264"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="24">
        <location>
            <position position="312"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="24">
        <location>
            <position position="408"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="25">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00031357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR001143-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005023478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="458" mass="51284" checksum="DE2837360B453726" modified="2022-01-19" version="1">MRCLNMIMAESLGLVTICLLGYLLSAECTVFLDRENATKILNRPKRYNSGKLEEFVRGNLERECMEEKCSFEEAREVFENTEKTTEFWKQYVDGDQCESNPCLHGGVCKDDINSYECWCQPGFEGKNCELYATCSIKNGRCKQFCKNSADNKVICSCTAGYRLAEDQKSCEPAVPFPCGKVSVSHASMKVTRAETIFSNMNYENSTEAETIWDNITEFNDLNRVVGGENAKPGQFPWQVLLHGKIAAFCGGSIINEKWVVTAAHCIEPGVKITVVAGEHNTEEIDHTEQKRNVIRAIPHHSYNATLNKYNHDIALLELDKPLTLNSYVTPICVADKDYTNIFLKFGSGYVSGWGRVFSRGRSASILQHLKVPLVDRATCLRSTKFTIHNNMFCAGFHEGGKDSCQGDSGGPHVTEVEGTSFLTGIISWGEECAVKGKYGIYTKVSRYVNWIKEKTKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PR63</accession>
    <name>A0A8C4PR63_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Protein-tyrosine kinase 2-beta</fullName>
            <ecNumber evidence="7">2.7.10.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">Calcium-dependent tyrosine kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">Calcium-regulated non-receptor proline-rich tyrosine kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">Cell adhesion kinase beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Focal adhesion kinase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Proline-rich tyrosine kinase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="34" type="primary">PTK2B</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="34 35" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22 36">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell projection</location>
            <location evidence="4">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
            <location evidence="6">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the protein kinase superfamily. Tyr protein kinase family. Fes/fps subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="7"/>
    <dbReference type="RefSeq" id="XP_014698784.1">
        <property type="nucleotide sequence ID" value="XM_014843298.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014698786.1">
        <property type="nucleotide sequence ID" value="XM_014843300.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044622792.1">
        <property type="nucleotide sequence ID" value="XM_044766857.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070361257.1">
        <property type="nucleotide sequence ID" value="XM_070505156.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070361258.1">
        <property type="nucleotide sequence ID" value="XM_070505157.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PR63"/>
    <dbReference type="Ensembl" id="ENSEAST00005025972.2">
        <property type="protein sequence ID" value="ENSEASP00005023944.2"/>
        <property type="gene ID" value="ENSEASG00005015727.2"/>
    </dbReference>
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            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00061333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00074713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00075069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00075402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00076919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00078211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00083453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005023944.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1009" mass="115674" checksum="E7A1CBB4ACD312B9" modified="2023-09-13" version="2">MSGVSEPLSRVKVGTLRRPEGPPEPMVVVPEDVEKEDVRILKVCFYSNSFNPGKNFKLVKCTVHTEIREVIASILLSGRIGPNIQLAECYGLRLKHMKSDEIHWLHPQMTVGEVQDKYECLHMEAEWRYDLQIRYLPEDFMESLKEDRTTLLYFYQQLRNDYMQRYASKVSEGMALQLGCLELRRFFKDMPHNALDKKSNFELLEKEVGLDLFFPKQMQENLKPKQFRKMIQQTFQQYASLREEECVMKFFNTLAGFANIDQETYRCELIQGWNITVDLVIGPKGIRQLTSQDTKPTCLAEFKQIKSIRCLPLEEGQAVLQLGIEGAPQSLSIKTSSLAEAENMADLIDGYCRLQGEHKGSLIIHPKKDGEKRNSLPQIPVLNLETQRSRLSESCSIESDIYAEIPDETLRRPGGPQYGIAREDVVLSRILGEGFFGEVYEGVYTNHKGEKISVAVKTCKKDCTLDNKEKFMSEAVIMKNLDHPHIVKLIGIIEEEPTWIIMELYPFGELGHYLERNKNSLKVLTLVLYSLQICKAMAYLESINCVHRDIAVRNILVASANCVKLGDFGLSRYIEDEEYYKASVTRLPIKWMAPESINFRRFTTASDVWMFAVCMWEILSFGKQPFFWLENKDVIGVLEKGDRLPKPDLCPPILYTLMTRCWDYDPSERPRFTELVCSLSDVYQMEKDIDTEQERNARYRPPKILEPTALQEPPPKPSRPKYRTPPQTNLLAPTLQFQVPEGLCASSPTLTSPMEYPSPVNSLHTPPLHRHNVFKRHSMREGDFVRPSSREEAQQLWEAERVKMRQILDKQQKQMVEDYQWLRQEEKSLDPMVYMNDKSPLTPEKEPGYTEFTGPPQKPPRLGAQSIQPTANLDRTDDLVYLNVMELVRAVLDLKNELCQLPPEGYVVVVKNVGLTLRKLIGSVDDLLPSLPSSSRTEIEGTQKLLNKDLAELINKMRLAQQNAVTSLSEECKRQMLTASHTLAVDAKNLLDAVDQAKVLANLAHPPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PRV6</accession>
    <name>A0A8C4PRV6_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Ral transcription factor IIIC subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GTF3C1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005027633.2">
        <property type="protein sequence ID" value="ENSEASP00005025443.2"/>
        <property type="gene ID" value="ENSEASG00005017333.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008664"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000995">
        <property type="term" value="F:RNA polymerase III general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042791">
        <property type="term" value="P:5S class rRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16169">
        <property type="entry name" value="Tau138_eWH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056467">
        <property type="entry name" value="eWH_GTF3C1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044210">
        <property type="entry name" value="Tfc3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035625">
        <property type="entry name" value="Tfc3-like_eWH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007309">
        <property type="entry name" value="TFIIIC_Bblock-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056428">
        <property type="entry name" value="WH_GTF3C1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15180">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15180:SF1">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04182">
        <property type="entry name" value="B-block_TFIIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24101">
        <property type="entry name" value="WHD_GTF3C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23704">
        <property type="entry name" value="WHD_GTF3C1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="General transcription factor 3C polypeptide 1 winged-helix" evidence="8">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="B-block binding subunit of TFIIIC" evidence="7">
        <location>
            <begin position="174"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="GTF3C1 extended winged-helix" evidence="9">
        <location>
            <begin position="615"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="472"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="594"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="724"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="828"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1063"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1202"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1607"/>
            <end position="1633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1828"/>
            <end position="1974"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="474"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="554"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="766"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="833"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="849"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="862"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1077"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1206"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1232"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1611"/>
            <end position="1623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF23704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025443.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="2114" mass="237972" checksum="8004B352C66BBEDD" modified="2023-09-13" version="2">MDALESLLDEVALEGLDGLCLPALWSRLETRVPPFPLPLEPHTQEFLWRALATHPGISFYEEPRERPDLQLQDRYEEIDLETGILESKRDPVPLEDVYPIHMILENKDGIQGSCRYFKERKNITSDIRTKSLQSRCTMVEAFGRWGKKLIIVASQDMRYRALIGLEGDPDLKLPDFSYCILERLGRSRWQGELQRDLHSTAFKVDAGKLHYHRKILNKNGLITMQSHVIRLPTGAQQHSILLLLNRFHADRRSKYDILMEKLSLVLSARSNQIETLGKLREELGLCERTFKRLYQYMLNAGLAKVVSLPLQEIHPERGPCKTKKGTDVMVRCLKLLKEFRRKVEDDHDQDDDEDEEVISKTVPPVDVVYERDMLTQTYELIERRGTKGISQAEIRVAMNVGKLEARMLCRLLQRFKVVKGFMEDEGRQRTTKYISCVFAEESDLSRQYEREKARSELLTTVSLASVQEESLLPEGEDTFLSESDSEEEGSSRSSKRRGRGPQGDMKSLSLCPGTQPRHSTPAKGGWKVVNLHPLKKQPASSPAAAEEKACRSSGGRNSLPEASSSTDPSVSYGSHCMKSNSGDIAVIEEVRLENPKESGSSQKTGKHGPGQDKPHETYRLLKRRNLIIEAVTNLRLIESLFTIQKMIMDQEKQEGVSTKCCKKSIVRLVRNLSEEGLLRLYRTTVIQDGIKKKVDLVVHPSMDQNDPLVRSAIEQVRFRISNSSTANRVKVPQPPAPQGEAEEESQGKEGPSGSGDSPSDASSKPESGRMKKADKKMGVTQLKNYKPVVVPGLGRSLGFLPKMPRLRMVHRFLWYLIYGHPASKTVEKLGLSSERRTGKQEPSRVGAQSSSRSDLETSSEAPPKDNQDGASREAEVELATETVYVDEASWTRYIPPIPVHRDFGFGWALVSDILLCLPLSIFVQIVQVSYKVDNLEEFLNDPLRKHTLIRFLPRPVRQQLLYKRRYIFSVVENFQKLCYMGLLQFGPTEKFQDKDQVFIFLKKNAVIVDTTICDPHYNLAHSSRPFEKRLYVLNSMQDVENYWFDLQCVCLNTPLGVVRYPRVRKSSPDQGSDEEGSLQKEQESAIDKHTLERKGAMMEYTTCVDEGLIPGDGLGAAGLDSSFYGHLKRNWIWTSYIINNAKRNSTSENGLTVRLQTFLSKRPLPLGAGGNGRLNFWGEARAGFELCADRAEQFVVDREPTLDRNRRVRGGRSQKRKRLKKDPGKKNKKKKKEELPEEKSKKLRYHDEADQSALQRMTRLRVTWSMQEDGLLILCRIASNILNTKVKGPFVPWQVVRDILHATFEESLDKTSHSVGRRSRYIVKNPQGYLNYKVCLAEVYQDKALVGEFMNRKCDYEDPKVCASEFKEFVEKLKEKFSSALRNPNLEIPDTLQELFARYRVLAIGDEKDQLSKEDELNSVDDIHFLVLQNLIQSTLALSDSQMKSCQSFQTFRLYQEYKDQILVKAFMEYQKRSLVNRRRVNHMLGPKKNRAVPFVPMSYQLSQTYYRIFTWRFPSTVCTESFQFFNKIRAAGKLDQPDNFSFKDQDNNDSTSDLVAFSLDGPGGHCVAALTLFSLGLVSVDVRIPEQIVVVDSSLVENEVIKSLGKDGALEEDEDEEEDLDEGSGGKRRSIEVKARQASHTNYLLMRGYCAPGIVSTRNLNPNDSIVVNSCQVKFRLRHSPAHTCLGPTATSLEELTVGTSCLPDTFTRLFSSPGDASSWEEFAHQMALSGYSPGDVAAALEVQGAIAAAGCFGVDRVALSRRFSAFERTDGARSRTFSDYVQDLLEQNQVLEVGSNTVRLVTMASAQPWLLHSVQLKRREDDACNHQARPLEGPSSKDSPSERQAPPLQGPRSTKRRASTDAARQSVGANSEGTQKPPAKRPTLQDVRLAPSPEPRAAEGAETHAPALPIAPEDTGAREPSPGASEAGQEDQEGLGVPSSPGREQLSCQAQLPEGSEDPRGSAEKSVAGSLSQAAQERDCESICFIGRPWRIVDGHLNTPVCKGMMEAVLYHIMTRPGVPESCLLQHYQGVLQPIAVLELLQGLEFLGCIRKRVLRKPAAVSLFSKPAAEEAEAPSSPSESTTVFYEPTLDCTLRLGRVFPHEVNWNKWVHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PS94</accession>
    <name>A0A8C4PS94_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane protease serine</fullName>
            <ecNumber evidence="10">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106843215</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_014715648.2">
        <property type="nucleotide sequence ID" value="XM_014860162.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PS94"/>
    <dbReference type="Ensembl" id="ENSEAST00005029259.2">
        <property type="protein sequence ID" value="ENSEASP00005026950.2"/>
        <property type="gene ID" value="ENSEASG00005018364.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106843215"/>
    <dbReference type="KEGG" id="eai:106843215"/>
    <dbReference type="GeneTree" id="ENSGT00940000163500"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.960:FF:000011">
        <property type="entry name" value="Transmembrane protease serine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.960">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017329">
        <property type="entry name" value="Pept_S1A_HAT/DESC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF17">
        <property type="entry name" value="SUPPRESSOR OF TUMORIGENICITY 14 PROTEIN HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037941">
        <property type="entry name" value="TMPRSS11ABCDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50024">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 10" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="15"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="domain" description="SEA" evidence="13">
        <location>
            <begin position="44"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="14">
        <location>
            <begin position="186"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11">
        <location>
            <position position="226"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11">
        <location>
            <position position="271"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11">
        <location>
            <position position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037941-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026950.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="417" mass="46374" checksum="4CBA1E352EB019F4" modified="2023-09-13" version="2">MYRHGISSERRSRPLWMMVLIFLGVMAILGVTIGLLVHFLAVGKTYYYQGDFHISGVTYNDSCENAASQASTDLNKDIETKMSDAFQNSSLYKEYTNSQVIKLLPYLNGSSVQLQLTFKFPPAKRDRMKTEIEAILHQILKDNMASWNAVPTSIKLIEISKADAEMLTNNCCGRRLTNSIIAGNRIVNGKNALAGAWPWQASMQWKGQHHCGASLISSRWLLSAAHCFSKKNNSEDWTVNFGAIVNRPHMTRKVQNIIFHENYSKPGVHDDIALVQLAEEVFFTKYVRRICLPEAKMKLSDNDSVVVTGWGTLYMNGPLPVILQQASVKIIDNKVCNAPHALAGLVTDTMLCAGFMSGEADACQNDSGGPLAYPDSRNIWHLVGIVSWGEGCGKKNKPGVYTRVTAYRDWITSKTGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I604</accession>
    <name>A0A9L0I604_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tetratricopeptide repeat protein 19, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TTC19</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Binds to the mature mitochondrial complex III dimer, after the incorporation of the Rieske protein UQCRFS1. Interacts with UQCRC1 and UQCRFS1. Interacts with ZFYVE26 and CHMP4B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TTC19 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0I604"/>
    <dbReference type="Ensembl" id="ENSEAST00005077244.1">
        <property type="protein sequence ID" value="ENSEASP00005035617.1"/>
        <property type="gene ID" value="ENSEASG00005017560.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009194"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034551">
        <property type="term" value="P:mitochondrial respiratory chain complex III assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000240">
        <property type="entry name" value="Tetratricopeptide repeat protein 19, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040395">
        <property type="entry name" value="TTC19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13143">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13143:SF6">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 19, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13374">
        <property type="entry name" value="TPR_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="82"/>
            <end position="102"/>
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    </feature>
    <feature type="coiled-coil region" evidence="14">
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            <begin position="384"/>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022946"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066255"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00074652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005035617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="428" mass="47047" checksum="FF466CEF596416BF" modified="2023-09-13" version="1">MERGAVTSACVTAHAGGNDDSSWYVPALRIRRALGVGGGASHGAVAPAVRRGHGAGMYRLLIQSLGRGVLRAAGRRCRGCPARPLPGPAGGRLPEVEVPPSRVAPRGRGPGLLPLLAALSWFSRPAAEERPAAGGAAAGDAADEAEAEIIQLLKRAKLSIMKDEPEEAELILHDALRLAYESENKKAITYTYDLMANLAYIRGQLEDAEKLFKATMSYLLGGGTQQEDNAIIEISLKLASIYAAQNRQEFALAGYEFCISTLEEKIEREKELAEDILSVDEKANTRLLLGMCLDAYAHYLLFSKQPSQAQRMYEKALQISEEMLGERHPQTIVLMSDLATTLDAQGRFDEACVYVQRASDLARQIEHPELHMLLSNLAAVLMHRERYAQAKEIYQEALKRAELKRDEVSVQHIREELAELSRKSRHLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I963</accession>
    <name>A0A9L0I963_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Vir like m6A methyltransferase associated</fullName>
        </submittedName>
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    <gene>
        <name evidence="8" type="primary">VIRMA</name>
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    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the vir family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014697147.1">
        <property type="nucleotide sequence ID" value="XM_014841661.3"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005043344.1">
        <property type="protein sequence ID" value="ENSEASP00005037209.1"/>
        <property type="gene ID" value="ENSEASG00005004018.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831452"/>
    <dbReference type="KEGG" id="eai:106831452"/>
    <dbReference type="CTD" id="25962"/>
    <dbReference type="GeneTree" id="ENSGT00390000002833"/>
    <dbReference type="OrthoDB" id="2011702at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031801">
        <property type="entry name" value="VIR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026736">
        <property type="entry name" value="Virilizer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23185">
        <property type="entry name" value="PROTEIN VIRILIZER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23185:SF0">
        <property type="entry name" value="PROTEIN VIRILIZER HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15912">
        <property type="entry name" value="VIR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Virilizer N-terminal" evidence="7">
        <location>
            <begin position="6"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="132"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="574"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1616"/>
            <end position="1635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1663"/>
            <end position="1812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="174"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="224"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="234"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="274"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="584"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1689"/>
            <end position="1698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1723"/>
            <end position="1748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1788"/>
            <end position="1802"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1803"/>
            <end position="1812"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF15912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005037209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1812" mass="202028" checksum="8EF444A7816C017B" modified="2023-09-13" version="1">MAVDSAMELLFLDTFKHPSAEQSSHIDVVRFPCVVYINEVRVIPPGVRAHSSLPDNRAYGETSPHTFQLDLFFNNVSKPSAPVFDRLGSLEYDENTSIIFRPNSKVNTDGLVLRGWYNCLTLAIYGSVDRVISHDRDSPPPPPPPPPPPQPQPSLKRNPKHADGEKEDQFNGSPPRPQPRGPRTPPGPPPPDDDEDDPMPLPVSGDKEEDAPHREDYFEPISPDRNSVPQEGQYSDEGEVEEEQQEEGEDDEDDVDVEDEEDEDEDDRHTVDSIPEEEEEDEEEEGEEDEEGEGDDGYEQISSDEDGIADLERETFKYPNFDVEYTAEDLASVPPMTYDPYDRELVPLLYFSCPYKTTFEIELSRMKDQGPDKENSGAIEASVKLTELLDLYREDRGAKWVTALEEIPSLIIKGLSYLQLKNTKQDSLSQLVDWTMQALNLQVALRQPIALNVRQLKAGTKLVSSLAECGAQGVMGLLQAGVISGLFELLFADHVSSSLKLNAFKALDSVISMTEGMEAFLRSRQNEKSGYQKLLELILLDQTVRVVTAGSAILQKCHFYEILSEIKRLGDHLAEKTSSVPNHSEPDHDTDTGLERTNPEYENEVEASMDMDLLESSNISEGEIEKLINLLEEVFHLMETAPHTMIQPPVKSFPTMARITGPPERDDPYPVLFRYLHSHHFLELVTLLLSIPVTSAHPGVLQATKDVLKFLAQSQKGLLFFMSEYEATNLLIRALCHLYDQDEEEGLQTDGVIDDAFALWLQDSTQTLQCITELFSHFQRCTASEETDHSDLLGTLHNLYLITFNPVGRSAVGHVFSLEKNLQSLITLMEYYSKEALGDSKSKKSVAYNYACILILVVVQSSSDVQMLEQHAASLLKLCKADENNAKLQELGKWLEPLKNLRFEINGIPNLIEYVKQNIDNLMTPEGVGLTTALRVLCNVACPPPPVEGQQKDLKWNLAVIQLFSAEGMDTFIRVLQKLNSILTQPWRLHVNMGTTLHRVTTISMARCTLTLLKTMLTELLRGGSFEFKDMRVPSALVTLHMLLCSIPLSGRLDSDEQKIQNDIIDILLTFTQGVNEKLTISEETLANNTWSLMLKEVLSSILKVPEGFFSGLILLSELLPLPLPMQTTQVIEPHDISVALNTRKLWSMHLHVQAKLLQEIVRSFSGTTCQPIQHMLRRICVQLCDLASPTALLIMRTVLDLIVEDLQSTSEDKEKQYTSQTTRLLALLDALASHKACKLAILHLINGTIKGDERYAEIFQDLLALVRSPGDSVIRQQCVEYITSILQSLCDQDIALILPNSSEGSISELEQLSNSLPNKELMASICDCLLAMLANSESSYNCLLTCVRTMMFLAEHDYGLFHLKSSLRKNSNALHSLLKRVISTFSKDTGELASSFLEFMRQILNSDAMGCCGDDSGLMEVEGAHPSRTMSINAAELKQLLQSKEESPENLFLELEKLVLEHSKDDDNLDSLLDNVVGLKQMLESSGDPLPLGDQDVEPVLSAPESLQNLFNNRTAYVLADVMDDQLKSMWFTPFQAEEIDTDLDLVKVDLIELSEKCCSDFDLHSELERSFLSEPSSPGRTKTTKGFKLGKHKHETFITSSGKSEYIEPAKRAHVVPPPRGRGRGGFGQGIRPHDIFRQRKQNTSRPPSMHVDDFVAAESKEVVPQDGIPPPKRPLKVSQKISSRGGFSGNRGGRGAFHSQNRFFTPPASKGNYSRREGTRGSSWSAQNTPRGTYNESRGGQSNFNRGPLPPLRPLSSTGYRPSPRDRASRGRGGLGPSWASANSGSGGSRGKFVSGGSGRGRHVRSFTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IA45</accession>
    <name>A0A9L0IA45_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106836894</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IA45"/>
    <dbReference type="Ensembl" id="ENSEAST00005067092.1">
        <property type="protein sequence ID" value="ENSEASP00005037654.1"/>
        <property type="gene ID" value="ENSEASG00005026742.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282496"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="CDD" id="cd15237">
        <property type="entry name" value="7tmA_OR2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="134"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="235"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="43"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005037654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="336" mass="37684" checksum="AAFB784CC8E389BB" modified="2023-09-13" version="1">MVDLRNRSTVTEFILVGFEQSSHSTRALLFAVFLALYSLAMAMNGLIIFITWTHPRLNSPMYFFLGHLSFLDVCFITTTTPQMLIHLVVQNHIVSFVSCMTQMYLVFCVGVAECILLAFMAYDRYVAICHPLSYAQIMSWHVCMGLVSTAWFFGLINGILLDYMTFRGPFCRDNHIENFFCEAPIVITLSCGDPQFSLRVIFAEAIVVLLSPMVLVVISYARILASILSRASSSGWGKAFSTCASHLTVVVFFHTSAMFSYMNPRSMHGPDKDKPFSLLYTVITPMCNPIIYSFRNKEMKGAMVRALGRTSLGPDIVFVASSLLEWKLLPPTLAEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0ICF7</accession>
    <name>A0A9L0ICF7_EQUAS</name>
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        <recommendedName>
            <fullName evidence="16">All-trans-retinol 13,14-reductase</fullName>
            <ecNumber evidence="15">1.3.99.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">All-trans-13,14-dihydroretinol saturase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">PPAR-alpha-regulated and starvation-induced gene protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">RETSAT</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>all-trans-13,14-dihydroretinol + A = all-trans-retinol + AH2</text>
            <dbReference type="Rhea" id="RHEA:19193"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:52075"/>
            <dbReference type="EC" id="1.3.99.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum membrane</location>
            <topology evidence="4">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the carotenoid/retinoid oxidoreductase family. CrtISO subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.23" evidence="15"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0ICF7"/>
    <dbReference type="Ensembl" id="ENSEAST00005078599.1">
        <property type="protein sequence ID" value="ENSEASP00005038584.1"/>
        <property type="gene ID" value="ENSEASG00005027102.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106838606"/>
    <dbReference type="KEGG" id="eai:106838606"/>
    <dbReference type="CTD" id="54884"/>
    <dbReference type="GeneTree" id="ENSGT00390000017613"/>
    <dbReference type="OrthoDB" id="38045at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051786">
        <property type="term" value="F:all-trans-retinol 13,14-reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000139">
        <property type="entry name" value="All-trans-retinol 13,14-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000178">
        <property type="entry name" value="All-trans-retinol 13,14-reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052206">
        <property type="entry name" value="Retinol_saturase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46091:SF1">
        <property type="entry name" value="ALL-TRANS-RETINOL 13,14-REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46091">
        <property type="entry name" value="BLR7054 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040331072" description="All-trans-retinol 13,14-reductase" evidence="20">
        <location>
            <begin position="23"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00077112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00080843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="609" mass="66943" checksum="2256844553ED05AB" modified="2023-09-13" version="1">MGLSLLLLAALLLAVLFKDYLGLFARSSPNPFSEDVRRPPAPLVTDREARKKVLKQTFSASRVPEKLDVVVIGSGFGGLAAAAILAKAGKRVLVLEQHTKAGGCCHTFGQDGLEFDTGIHYVGCMEEGSPGRFILDQITEGQLDWAPLSSPFDIMVLEGPSGQKEFPMYKGKKAYIQGLKEKFPQEEAAINKYMKLVQMVSRGAIHAVLLKIIPLPVAQFLNKCGLLTRFSPFLRASTQSLAEVLRQLPASPELQAVLSYIFPTYGVTPSHSTFSMHALLVEHYIEGAFYPRGGSSEIAFHTIPVIQRAGGAVLTRAPVQSVLLDSAGKACGVSVKTNRELVNICCPIVISSAGLFNTYKYLLPEKARCLPGVKQQLEMVRPSLSMISVFICLRGTKKDLGLQTTNYYVYFDTDMDKAMEHYYSLPREKAAAHLPFLFISSSSAKDSTWEDRFPDRSTMIVLIPSSYAWFEEWREEPKGKRSSDYETLKNSFVEASLSVVMKLFPQLEGKVDKVTGGSPLTTQFYLAAPQGACYGAAHDLARLQPHVMASLRAQSPIPNLYLTGQDIFTCGLMGTLQGALLCSSAILKRNLYSDLKKLGSRIQAQKKKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IIQ0</accession>
    <name>A0A9L0IIQ0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">BET1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">BET1L</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Vesicle SNARE required for targeting and fusion of retrograde transport vesicles with the Golgi complex. Required for the integrity of the Golgi complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of a SNARE complex consisting of STX5, YKT6, GOSR1 and BET1L. Interacts with STX5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IIQ0"/>
    <dbReference type="Ensembl" id="ENSEAST00005065034.1">
        <property type="protein sequence ID" value="ENSEASP00005040830.1"/>
        <property type="gene ID" value="ENSEASG00005031609.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160208"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15853">
        <property type="entry name" value="SNARE_Bet1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000038">
        <property type="entry name" value="BET1-like protein isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039899">
        <property type="entry name" value="BET1_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="298"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="14">
        <location>
            <begin position="226"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="105"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005040830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="322" mass="34002" checksum="2D5AE3B977E52164" modified="2023-09-13" version="1">MPHCGGGDRSTVLLPCLARRALGQRPDRCPGPAPPALPLQELPWRPLRARSSRARAPLGPRGRLCVATLPRLSAGKGPVSSSQLGPDTALEGVAATASLEAATGVKKPNAQAARVGRRNKRQATSVPTASPGRPAPEPRTEGAGLSRKFLSRGCATSEEVGAKPARWRTGRGVSAGEQTRVLRAPPSRAVEGSRGPASAPRSCGVCVPGARTAPPVPAQSPGAVEEILDRENKRMADSLASKVTRLKSLALDIDRDAEDQNRYLDGMDTDFTSMTSLLTGSVKRFSMMARSGRDNRKLLCGVAVGLIVAFFILSYFLSRSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IJ69</accession>
    <name>A0A9L0IJ69_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Cyclin-G-associated kinase</fullName>
            <ecNumber evidence="6">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">DnaJ homolog subfamily C member 26</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">GAK</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. May play a role in clathrin-mediated endocytosis and intracellular trafficking, and in the dynamics of clathrin assembly/disassembly.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
            <location evidence="4">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="6"/>
    <dbReference type="RefSeq" id="XP_044623966.1">
        <property type="nucleotide sequence ID" value="XM_044768031.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005057231.1">
        <property type="protein sequence ID" value="ENSEASP00005041000.1"/>
        <property type="gene ID" value="ENSEASG00005022610.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106825426"/>
    <dbReference type="KEGG" id="eai:106825426"/>
    <dbReference type="CTD" id="2580"/>
    <dbReference type="GeneTree" id="ENSGT00940000159527"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
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    <sequence length="1306" mass="142562" checksum="F70AD326526ED168" modified="2023-09-13" version="1">MSLLQSALDFLAGPGSLGGAAGRDQTDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDLGSGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNVVQFCSAASIGKEESDTGQAEFLLLTELCRGQLVEFLKKVESKGPLSCDTILKIFYQTCRAVQHMHRQKPPIVHRDLKVENLLLSNQGTIKLCDFGSATTTLHYPDYSWSAQKRALVEDEITRNTTPMYRTPEIVDLYSNFPISEKQDIWALGCILYLLCFGQHPFEDGAKLRIVNGKYSIPPNDTRYSVFHGLIRAMLKVNPEERLSITELVNQLQEIAAARNVNPKSPITELLEQNGGCGNAALSRGLSPPGGPTSSSYSGGLVVAEYDQPYGGFLDILRGGTERLFTNIKDTSSKVIQSVANYAKGDLDISYITSRIAVMSFPAEGVESAIKNNIEDVRLFLDSKHPGHYAVYNLSPRMYRPSKFHNRVSECGWAARRAPSLHSLYSICRNMHSWLRQDHKNVCVVHCMDGRAASAVAVCSFLCFCRLFSTAEAAVYMFSMKRCPPGIWPSHKRYIEYMCDMVAEEPITPHSKPILVKAVVMTPVPLFSKQRNGCRPFCEVYVGDERVTTTSQEYDRMRDFKIEDGKAVIPLGITVQGDVLIIIYHARSTLGGRLQAKMASMKMFQIQFHTGFVPRNATTVKFAKYDLDACDIQEKYPDLFQVNLEVEVEPRDRPSREAPPWESASLRGLNPKILFSSREEQQDILSKFGKPELPRQPGSTAQYDAEAGSPDTEPTESDSPQSSSADTPHFLHTLDWHEERDSETGPGSNIAKESQSAPTEDGGESEPSDEEAVPSSAESRELPDEDSSGPPARTQESELIFDVDAPAAPQEPALQEDGVDLLGLHSEAGLAPPLQTCNVPPSNADLLSCLLGAPVAAPGSSPGDLLGSEVPPLFASPAPPLSARSTPREEPAVSADPFDPLLLPADPDAQPSSKPDLFGEFLNSDSLAAPPASFPSTHSAPPPACSPDFLHLGDLPAEPGKMAASTSHPDLLGGWDAWAEAPAPTPATEGPLLAPGGQPPPPGPSASRTKSQSLDPFADLGDLSPGLPGSTAGFAPGAFGPKAAPPPKGGGSWQTSRPPAQGAPWPPQARPPPKTSAQPRPNYTTNFSVIGGREERGVRAPSFAQKPRVSENDFEDLLSNQGFSSKSDKKGPRTIAEMRRQDQARDTDPLKLKLLDWTEGKEKNIRALLSTLHTVLWDGESRWTPVGMADLVTPGQVKKQYRRAVLVVHPDKAAGQPYEQYARMIFMELSDAWAEFESQGSRPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IJH1</accession>
    <name>A0A9L0IJH1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 26C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">VPS26C</name>
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    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the commander complex that is essential for endosomal recycling of transmembrane cargos; the commander complex is composed of the CCC subcomplex and the retriever subcomplex. Component of the retriever complex, which is a heterotrimeric complex related to retromer cargo-selective complex (CSC) and essential for retromer-independent retrieval and recycling of numerous cargos such as integrin alpha-5/beta-1 (ITGA5:ITGB1). The recruitment of the retriever complex to the endosomal membrane involves CCC and WASH complexes. In the endosomes, drives the retriever and recycling of NxxY-motif-containing cargo proteins by coupling to SNX17, a cargo essential for the homeostatic maintenance of numerous cell surface proteins associated with processes that include cell migration, cell adhesion, nutrient supply and cell signaling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the commander complex that is essential for endosomal recycling of transmembrane cargos; the commander complex is composed of the CCC subcomplex and the retriever subcomplex. Component of the heterotrimeric retriever complex consisting of VPS26C, VPS29 and VPS35L; within the complex interacts with VPS35L. Interacts with SNX17 (via C-terminus); the interaction is direct and associates SNX17 with the retriever complex. Interacts with SNX31; the interaction is direct.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS26 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014697068.1">
        <property type="nucleotide sequence ID" value="XM_014841582.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IJH1"/>
    <dbReference type="Ensembl" id="ENSEAST00005046277.1">
        <property type="protein sequence ID" value="ENSEASP00005041033.1"/>
        <property type="gene ID" value="ENSEASG00005006082.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831409"/>
    <dbReference type="CTD" id="10311"/>
    <dbReference type="GeneTree" id="ENSGT00950000183064"/>
    <dbReference type="OMA" id="CVTMPIT"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000008">
        <property type="entry name" value="Down syndrome critical region protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000009">
        <property type="entry name" value="Down syndrome critical region protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028934">
        <property type="entry name" value="Vps26-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12233">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 26 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03643">
        <property type="entry name" value="Vps26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00067597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00093474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005041033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="297" mass="33033" checksum="0BFB980A5C10F2CF" modified="2023-09-13" version="1">MGTSLDIKIKRANKVYHAGEMLSGVVVVSGKDPVQHQGLFLTVEGTVNLQLSAKSVGVFEAFYNSVKPIQIINSTIEMVKPGKFPPGKTEIPFEFPLHVKGNKVLYETYHGVFVNIQYTLRCDMKRSLLAKDLTKTCEFIVHSAPQKGKLTPSPVDFTITPETLQNVKERALLPKFLIRGHLNSTNCVITQPLTGELVVESSEAAIRSIELQLVRVETCGCAEGYARDATEIQNIQIADGDVCRSLSVPIYMVFPRLFTCPTLETTNFKVEFEVNIVVLLHADHLITENFPLKLCRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IMM2</accession>
    <name>A0A9L0IMM2_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Kelch like family member 40</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">KLHL40</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a key regulator of skeletal muscle development. The BCR(KLHL40) complex acts by mediating ubiquitination and degradation of TFDP1, thereby regulating the activity of the E2F:DP transcription factor complex. Promotes stabilization of LMOD3 by acting as a negative regulator of LMOD3 ubiquitination; the molecular process by which it negatively regulates ubiquitination of LMOD3 is however unclear.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the BCR(KLHL40) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL40 and RBX1. Interacts with LMOD3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
            <location evidence="1">A band</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Myofibril</location>
            <location evidence="2">Sarcomere</location>
            <location evidence="2">I band</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the KLHL40 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IMM2"/>
    <dbReference type="Ensembl" id="ENSEAST00005061859.1">
        <property type="protein sequence ID" value="ENSEASP00005042201.1"/>
        <property type="gene ID" value="ENSEASG00005007920.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156360"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031672">
        <property type="term" value="C:A band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031674">
        <property type="term" value="C:I band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032435">
        <property type="term" value="P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031397">
        <property type="term" value="P:negative regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048741">
        <property type="term" value="P:skeletal muscle fiber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000006">
        <property type="entry name" value="Kelch repeat and BTB domain-containing 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000037">
        <property type="entry name" value="Kelch-like family member 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF22">
        <property type="entry name" value="KELCH-LIKE PROTEIN 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="12">
        <location>
            <begin position="93"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="11"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="328"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="336"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005042201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="683" mass="76136" checksum="70BD827B32A45A3D" modified="2023-09-13" version="1">MDICALEHLQTPSPSLKGGLGFRKHPEPREDSCADSNPTAKQERTERRPLAPSLHPRPPAMALGLEQAEEQRLYQQTLLQDGLKDMLDHGKFLDCVVRVGEREFPCHRLVLAACSPYFRARFLAEPERAGELRLEEVSPDVVAQVLHYLYTSEIALDEASVQDLFAAAHRFQIPSIFTICVSFLQKRLCLSNCLAVFRLGLLLDCARLAVAARDFICARFSLVARDADFLGLSADELIAIISSDGLNVEKEEAVFEAVMRWASSGDAEAQAERQRALPTVFESVRFRLLPRAFLESRVERHPLVRAQPELLRKVQMVKDAHEGRLTVLRKKKKKGKEAAGPKAADKGTADAKAEEDEEEAERVLPGILNDTLRFGMFLQDLIFMISEEGAVAYDPAANECYCASLSTQIPKNHVSLVTKENQVFVAGGLFYNEDNKEDPMSAYFLQFDHLDSEWLGMPPLPSPRCLFGLGEALNAIYVVGGRELKDGESSLDSVLCYDRQSFKWGESDPLPYAVYGHAVLSHMDLVYVIGGKGRDRKCLNKMCVYDPKKFEWKELAPMQTARSLFGATIHDGRIFVAAGVTDTGLTSSAEVYSIADNKWAPFEAFPQERSSLSLVSLAGTLYAIGGFATLETESGELVPTELNDIWRYNEDEKKWEGVLREIAYAAGATFLPVRLNVLRLTKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IQI1</accession>
    <name>A0A9L0IQI1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Adenosine receptor A2</fullName>
        </recommendedName>
    </protein>
    <gene>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
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                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="13 14">Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.</text>
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        <subcellularLocation>
            <location evidence="1 14">Cell membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
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    </dbReference>
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    <dbReference type="KEGG" id="eai:106838859"/>
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        <property type="component" value="Chromosome 13"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="Adeno_A2B_rcpt"/>
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        <property type="entry name" value="Adenosn_rcpt"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8 14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="9 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="12"/>
            <end position="32"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IS91</accession>
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        <alternativeName>
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        <name type="scientific">Equus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Fang"/>
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                <person name="Zhao"/>
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                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
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                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
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                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Metal transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the ACDP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0ISX4"/>
    <dbReference type="Ensembl" id="ENSEAST00005040485.1">
        <property type="protein sequence ID" value="ENSEASP00005044061.1"/>
        <property type="gene ID" value="ENSEASG00005030817.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161102"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010960">
        <property type="term" value="P:magnesium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04590">
        <property type="entry name" value="CBS_pair_CorC_HlyC_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.580.10:FF:000001">
        <property type="entry name" value="Putative metal transporter CNNM3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045095">
        <property type="entry name" value="ACDP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002550">
        <property type="entry name" value="CNNM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044751">
        <property type="entry name" value="Ion_transp-like_CBS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064">
        <property type="entry name" value="METAL TRANSPORTER CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12064:SF27">
        <property type="entry name" value="METAL TRANSPORTER CNNM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25562">
        <property type="entry name" value="CNBH_CNNM2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01595">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51846">
        <property type="entry name" value="CNNM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040373472" description="Metal transporter" evidence="14">
        <location>
            <begin position="27"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="264"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="CNNM transmembrane" evidence="16">
        <location>
            <begin position="139"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="15">
        <location>
            <begin position="395"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU369091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005044061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="669" mass="72093" checksum="DFFAF284A70E6D08" modified="2023-09-13" version="1">RAAQAMAAAGLLGWLLAAFCLGNADGEAAPGPRVLGVCLEEDGAAGAGWASGEEVQAAPEATFRLRLFGSGFANSSWSWVAPEGAGCPEGGPAAAPEEAAAVPTGEWRALLRLPAGCVVRPHSALLAVRVEPGGAAGEEAAPPWALGLGAVGLLALAALARGLQLSALALVPAEVQVLRESGSEAERAAARRLEPVRRWAGCALGALLLLASLAQAALAVLLYRAAGQRAVPAVLGSAGLVFLVGEVVPAAVSGRWALALAPRALVLSRLAVLLTLPVALPVGQLLELAARPGRLRERVLELARGGGDPYSDLSKGVLRCRTVEDVLTPLEDCFMLDASAVLDFGVLASIMQSGHTRIPVYEEERSNIVDMLYLKDLAFVDPEDCTPLSTITRFYNHPLHFVFNDTKLDAVLEEFKRGKSHLAIVQKVNNEGEGDPFYEVLGLVTLEDVIEEIIKSEILDESEDYRDAAVRKKHTYLSAPLRRKEEFSLFKVSDEDYKVKISPQLLLATQRFLSREVDVFSPLRISEKVLLHLLKHPSVNQEVRFDESNRLAAHHYLYQRSQPVDYFILILQGRVEVEIGKEGLKFENGAFTYYGVSALTAPSSVHQSPVSSLQSIRHDLQPEPADSTRLSAYCPDYTVRALSDLQFIKVTIWAAHSSWLLASLVSPRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0ITQ9</accession>
    <name>A0A9L0ITQ9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Caspase-2</fullName>
            <ecNumber evidence="9">3.4.22.55</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Protease ICH-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CASP2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Strict requirement for an Asp residue at P1, with 316-Asp being essential for proteolytic activity and has a preferred cleavage sequence of Val-Asp-Val-Ala-Asp-|-.</text>
            <dbReference type="EC" id="3.4.22.55"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a p18 subunit and a p12 subunit. Forms a complex named the PIDDosome with PIDD1 and CRADD. Interacts with NOL3 (via CARD domain); inhibits CASP2 activity in a phosphorylation-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 13">Belongs to the peptidase C14A family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.55" evidence="9"/>
    <dbReference type="RefSeq" id="XP_044624138.1">
        <property type="nucleotide sequence ID" value="XM_044768203.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0ITQ9"/>
    <dbReference type="Ensembl" id="ENSEAST00005050923.1">
        <property type="protein sequence ID" value="ENSEASP00005040474.1"/>
        <property type="gene ID" value="ENSEASG00005009238.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106833854"/>
    <dbReference type="CTD" id="835"/>
    <dbReference type="GeneTree" id="ENSGT00940000156657"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905369">
        <property type="term" value="C:endopeptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071260">
        <property type="term" value="P:cellular response to mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030330">
        <property type="term" value="P:DNA damage response, signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035234">
        <property type="term" value="P:ectopic germ cell programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097192">
        <property type="term" value="P:extrinsic apoptotic signaling pathway in absence of ligand"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001235">
        <property type="term" value="P:positive regulation of apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00032">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1460:FF:000009">
        <property type="entry name" value="Caspase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000024">
        <property type="entry name" value="caspase-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1470:FF:000001">
        <property type="entry name" value="Putative caspase-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1470">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001315">
        <property type="entry name" value="CARD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029030">
        <property type="entry name" value="Caspase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033139">
        <property type="entry name" value="Caspase_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016129">
        <property type="entry name" value="Caspase_his_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002398">
        <property type="entry name" value="Pept_C14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011600">
        <property type="entry name" value="Pept_C14_caspase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002138">
        <property type="entry name" value="Pept_C14_p10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001309">
        <property type="entry name" value="Pept_C14_p20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015917">
        <property type="entry name" value="Pept_C14A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47901:SF7">
        <property type="entry name" value="CASPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47901">
        <property type="entry name" value="CASPASE RECRUITMENT DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00619">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00656">
        <property type="entry name" value="Peptidase_C14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038001">
        <property type="entry name" value="Caspase_ICE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00376">
        <property type="entry name" value="IL1BCENZYME"/>
    </dbReference>
    <dbReference type="SMART" id="SM00114">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00115">
        <property type="entry name" value="CASc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52129">
        <property type="entry name" value="Caspase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50209">
        <property type="entry name" value="CARD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01122">
        <property type="entry name" value="CASPASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01121">
        <property type="entry name" value="CASPASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50207">
        <property type="entry name" value="CASPASE_P10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50208">
        <property type="entry name" value="CASPASE_P20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0865">Zymogen</keyword>
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        <location>
            <begin position="32"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p20" evidence="16">
        <location>
            <begin position="205"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Caspase family p10" evidence="15">
        <location>
            <begin position="361"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="333"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="284"/>
        </location>
    </feature>
    <feature type="active site" evidence="12">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00066475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR038001-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005040474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="459" mass="51425" checksum="08E2CB72CACBE9A2" modified="2023-09-13" version="1">MAAPSAASLPALQQKGLMAADRGRRILGVCGMHPDHQEALKKNRVVLAKQLLLSELLEHLLEKDIITLEMRELIQAKVTSFGQNVELLNLLPKRGPQAFEAFCVALRETKQGHLEDLLLTTLSGLQHVLPPVWSPRNMLSCDYDLSLPFPVYESCPPHKQLRLSTDTVEHSLDNGDGPPCLQVKPCTPEFYQAHYQLAYRLQSRPRGLALVLSNVHFTGEKDLEFRSGGDVDHSTLVTLFKLLGYNVHVLLDQTAKEMQEKLQNFAQLPAHRVTDSCIVALLSHGVEGGIYGVDGKLLQLQEVFRLFDNANCPSLQNKPKMFFIQACRGDETDRGVDQQDGKNHAKPPECEESDAGKEELLKMRLPTRSDMICGYACLKGTAAMRNTKRGSWYIEALTQVFSERACDMHVADMLVKVNALIKEREGYAPGTEFHRCKEMSEYCSTLCHHLYLFPGHPPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IU21</accession>
    <name>A0A9L0IU21_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">SPATA31-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SPATA31 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IU21"/>
    <dbReference type="Ensembl" id="ENSEAST00005045517.1">
        <property type="protein sequence ID" value="ENSEASP00005041281.1"/>
        <property type="gene ID" value="ENSEASG00005029119.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183043"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027970">
        <property type="entry name" value="SPATA31-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21859">
        <property type="entry name" value="ACROSOME-SPECIFIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21859:SF51">
        <property type="entry name" value="RIKEN CDNA 1700014D04 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15371">
        <property type="entry name" value="DUF4599"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="SPATA31-like" evidence="7">
        <location>
            <begin position="30"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="92"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="107"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="135"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF15371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005041281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="258" mass="27291" checksum="872DA6CB968C32D4" modified="2023-09-13" version="1">MGVATSVRPARAPYICSGPGCGYVSPVSFSPRGQHRDTICFRQLLCPDPSCEVCNSTPVEVNRLLFLEDLEDDTASVSSLASTASGTESSFTLSSAFSEVPPGDLTPSPPPDPSPPPPSVLSPNPTTPLADFLSPSPPGHSMPPEPSPPLESKFPADHCPPRPLALPPLPPHDTQATGPILQPEATLSLNTIFSLDRTLSQDINPLPNLSQIINPNDSLACHHAPPSLSVSPPTDHPLTVTRSSFHLIEVCSRELISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IVW3</accession>
    <name>A0A9L0IVW3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sodium/mannose cotransporter SLC5A10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Sodium/glucose cotransporter 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Solute carrier family 5 member 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">SLC5A10</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Electrogenic Na+-coupled sugar symporter that actively transports D-mannose or D-fructose at the plasma membrane, with a Na+ to sugar coupling ratio of 1:1. Transporter activity is driven by a transmembrane Na+ electrochemical gradient set by the Na+/K+ pump. Exclusively recognizes sugar substrates having a pyranose ring with an axial hydroxyl group on carbon 2. Has likely evolved to enable renal reabsorption of D-mannose, an important constituent of oligosaccharide chains of glycoproteins. Contributes to dietary D-fructose reabsorption from glomerular filtrate across the brush border of the kidney.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>D-fructopyranose(out) + Na(+)(out) = D-fructopyranose(in) + Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72915"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:37714"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:72916"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>D-mannose(out) + Na(+)(out) = D-mannose(in) + Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72907"/>
            <dbReference type="ChEBI" id="CHEBI:4208"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:72908"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IVW3"/>
    <dbReference type="Ensembl" id="ENSEAST00005066823.1">
        <property type="protein sequence ID" value="ENSEASP00005041239.1"/>
        <property type="gene ID" value="ENSEASG00005017607.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159416"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="SODIUM_MANNOSE COTRANSPORTER SLC5A10"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="12" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 20" id="KW-1133">Transmembrane helix</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="20"/>
            <end position="37"/>
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    <feature type="transmembrane region" description="Helical" evidence="20">
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    <feature type="transmembrane region" description="Helical" evidence="20">
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    <feature type="transmembrane region" description="Helical" evidence="20">
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        <source>
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        <source>
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    </evidence>
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            <dbReference type="RuleBase" id="RU362091"/>
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    </evidence>
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        <source>
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            <dbReference type="Ensembl" id="ENSEASP00005041239.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="559" mass="60552" checksum="9DF5588F8CD4004F" modified="2023-09-13" version="1">ANNSTAHSHASGPQLSVADIIVITVYFALNVAVGIWSSCRASRNTVSGYFLAGRDMTWWPIGASLFASSEGAGLFIGLAGSGAAGGLAVAGFEWNATYVLLALAWVFVPIYISSEIVTMPEYLQKRFGGQRIRMYLSVLSLLLSVFTKISIDLYAGALFVHICLGWNFYLSTILMLVITALYTIAGGLAAVIYTDALQTLIMVVGAVILTIKAFDQIGGYEQLAAAYSQAVPTRIIANTTCHLPRADAMHMFRDPYTGDLPWTGMTFGLTIMATWYWCTDQVIVQRSLSARDLNHAKAGSILASYLKMLPMGLMIMPGMISRALFPGLRGLMIAVMMAALMSSLTSIFNSSSTLFTMDIWRRLRPRAGERELLLVGRLVIVVLIGVSVAWIPVLQGSNSGQLFIYMQSVTSSLAPPVTAVFVLGIFWQRANEQGAFWGLMVGLAVGATRLVLEFLHPAPPCGAPDTRPAVLGSMHYLHFAIALFVLSGAVVVAGSLLTPPPESVQIENLTWWTLARALPPGAKTSDSRTAQKYAFWARVCGFNAILLVCVNIFFYAYFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IW47</accession>
    <name>A0A9L0IW47_EQUAS</name>
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        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
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    <gene>
        <name evidence="8" type="primary">LOC106822082</name>
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    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="nucleotide sequence ID" value="XM_014827102.3"/>
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        <property type="protein sequence ID" value="ENSEASP00005045104.1"/>
        <property type="gene ID" value="ENSEASG00005037691.1"/>
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        <property type="component" value="Chromosome 17"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Solute carrier family 22 member 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24064">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 22 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="261"/>
            <end position="280"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="350"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="379"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="411"/>
            <end position="428"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="466"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="498"/>
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        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="98"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="523"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="531"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
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            <dbReference type="Ensembl" id="ENSEASP00005045104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="552" mass="61826" checksum="FBED7EB70E762456" modified="2023-09-13" version="1">MAFQDLLDQVGGLGRFQILQIVFFCISSLIVYPHMLLENFTAAVPGHRCWVHILDNDTVSVNGTGILSQDAVLRISIPLDSNLRPEKCHRFLHPQWHLLHLNGTFPNMSELDMEACVNGWVYDQSSFSSTIVTEWDLVCEFQLLNSVSKFLFMAGTLVGSIIYGHLSDRFGRRLILRWCLFQFAIADTCAAFAPTFLIYCSLRFLAGFSITTIMLNSTMLIVEWTVPRFQAMGTTMKTCANSIGQIISGGLAFAIRDWRTLQLVVSLPVFLIFLSSRWLMESARWLIITNKPEDGLKELRKAAHRNGKKDVGDTLTLEVLTSTMQEELEAARTKHTVCDLFRTPNLRKRICLLSFVRFATFIPFYGLSLHLQHLGKNIFLFQVLFGVVTLPSNYVALLALNHLGRRVSQMLFLFLLGISILAIIFVPQEMQTPRVFLASLGIGVSSAAITSALAHGNELIPTVIRAIGLGIIGIAGNIGAALAPLLMILVVYSPRLPWIIYGVFPILAGLVVRLLPETRDQPLPDSIQDVENERKSWRKAKQEDISMKVTPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IW76</accession>
    <name>A0A9L0IW76_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Uridine-cytidine kinase</fullName>
            <ecNumber evidence="10">2.7.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">UCK2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>cytidine + ATP = CMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24674"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17562"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>uridine + ATP = UMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16825"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16704"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the uridine kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.48" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0IW76"/>
    <dbReference type="Ensembl" id="ENSEAST00005067263.1">
        <property type="protein sequence ID" value="ENSEASP00005045236.1"/>
        <property type="gene ID" value="ENSEASG00005010482.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01020000230412"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004849">
        <property type="term" value="F:uridine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02023">
        <property type="entry name" value="UMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000297">
        <property type="entry name" value="Uridine-cytidine kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006083">
        <property type="entry name" value="PRK/URK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000764">
        <property type="entry name" value="Uridine_kinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004018">
        <property type="entry name" value="PRK05480.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00235">
        <property type="entry name" value="udk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00485">
        <property type="entry name" value="PRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00988">
        <property type="entry name" value="URIDINKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribulokinase/uridine kinase" evidence="12">
        <location>
            <begin position="22"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005045236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="266" mass="29943" checksum="10C0AF4811691D85" modified="2023-09-13" version="1">MAGDSEQTLQNHQQPNGGEPFLIGVSGGTASGKSSVCAKIVQLLGQNEVDYRQKQVVILSQDSFYRVLTSEQKAKALKGQFNFDHPVCFPSDAFDNELIFKTLKEITEGKTVQIPVYDFVSHSRKEETVTVYPADVVLFEGILAFYSQEVRDLFQMKLFVDTDADTRLSRRVLRDISERGRDLEQILSQYITFVKPAFEEFCLPTKKYADVIIPRGADNLVAINLIVQHIQDILNGGISKRQTNGYLNGYTPSRKRQASESSSRPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IYT9</accession>
    <name>A0A9L0IYT9_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Sortilin related VPS10 domain containing receptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SORCS1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS10-related sortilin family. SORCS subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IYT9"/>
    <dbReference type="Ensembl" id="ENSEAST00005077872.1">
        <property type="protein sequence ID" value="ENSEASP00005046161.1"/>
        <property type="gene ID" value="ENSEASG00005015008.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234563"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.270:FF:000001">
        <property type="entry name" value="Sortilin related VPS10 domain containing receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000083">
        <property type="entry name" value="Sortilin-related VPS10 domain containing receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.80:FF:000001">
        <property type="entry name" value="Sortilin-related VPS10 domain-containing receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031777">
        <property type="entry name" value="Sortilin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031778">
        <property type="entry name" value="Sortilin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006581">
        <property type="entry name" value="VPS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050310">
        <property type="entry name" value="VPS10-sortilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106">
        <property type="entry name" value="SORTILIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12106:SF8">
        <property type="entry name" value="VPS10 DOMAIN-CONTAINING RECEPTOR SORCS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00801">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15902">
        <property type="entry name" value="Sortilin-Vps10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15901">
        <property type="entry name" value="Sortilin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00602">
        <property type="entry name" value="VPS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110296">
        <property type="entry name" value="Oligoxyloglucan reducing end-specific cellobiohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="PKD domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="860"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="10">
        <location>
            <begin position="594"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005046161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="945" mass="106723" checksum="54C81EEC9EC5ECEF" modified="2023-09-13" version="1">IMLLTDPEIESSLLISSDEGATYQKYRLNFYIQSLLFHPKQEDWILAYSQDQKLYSSAEFGRRWQLIQEGVAPNRFYWSVMGSNKEPDLVHLEARTVDGHSQYLTCRMQNCTEANRHKPFPGYIDPDSLIVQDDYVFVQLTSGGRPHYYVSYRRNAFAQMKLPKYALPKDMHVISTDENQVFAAVQEWNQNDTYNLYISDTRGVYFTLALENVQSSRGPEGNVMIDLYEVAGIKGMFLANKKIDNQVKTFITYNKGRDWRLLQAPDTDLRGDPVQCLLPYCSLHLHLKVSENPYTSGIIASRDTAPSIIVASGNIGSELSDSDISMFVSSDAGNTWRQIFEEEHSVLYLDQGGVLVAMKHTSLPIRHLWLSFDEGRSWSKYSFTSIPLFVDGVLGEPGEETLIMTVFGHFSHRSEWQLVKVDYKSIFDRRCAEEDYRPWQLHSQGEACIMGAKRIYKKRRSERKCMQGKYAGAMESEPCVCTEADFDCDYGYERHSNGQCLPAFWFNPSSLSKDCSLGQSYLNSTGYRKVVSNNCTDGVREQYTAKPQKCPGKAPRGLRIVTADGKLTAEQGHNVTLMVQLEEGDVQRTLIQVDFGDGIAVSYVNLSSMEDGIKHVYHNVGIFRVTVQVDNSLGSDSAVLYLHVTCPLEHVHLSLPFVTTKNKEVNATAVLWPSQVGTLTYVWWYGNNTEPLITLEGSISFKFTSEGMNTITVQVSAGNAILQDTKTIAVYEEFRSLRLSFSPNLDDYNPDIPEWRRDISRVIKKSLVEATGVPSQHILVAVLPGLPTAAELFVLPYQDPTGENRRSAEDLEQISELLIHKLNQNLVHFELKPGVQVFVHAAHLTAAPLVDLTPTHSGSAMLMLLSVVFVGLAVFVIYKFKRKIPGINVYAQMQNEKEREMVSRVSHTESRSHIPQTELRRPGQLIDEKVESQLIGTSLSTLQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J071</accession>
    <name>A0A9L0J071_EQUAS</name>
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        <submittedName>
            <fullName evidence="2">MYC target 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">MYCT1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J071"/>
    <dbReference type="Ensembl" id="ENSEAST00005066328.1">
        <property type="protein sequence ID" value="ENSEASP00005045783.1"/>
        <property type="gene ID" value="ENSEASG00005031624.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011642"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061484">
        <property type="term" value="P:hematopoietic stem cell homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029180">
        <property type="entry name" value="Myc_target_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14869">
        <property type="entry name" value="MYC TARGET PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14869:SF0">
        <property type="entry name" value="MYC TARGET PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15179">
        <property type="entry name" value="Myc_target_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005045783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="197" mass="22035" checksum="F128272A62D3B82B" modified="2023-09-13" version="1">MVFCTAAQVDKDVTHKPWFIFPNTVLYEDLIMSFTVSMAIGLVLGGMIWALFVCLSRRRASASISQWSSSRRPRSSYTHGLNRTGFYRHSGCERRSNLSLASLTFQRQASLEQANSFPRKSSFRASTFHPFLQSPPLPVETDSQLMTLPSSNTSPTINTSHSLSRPDFHWSSNSLRIGLSTPPPPAYESIIKAFPDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J0P5</accession>
    <name>A0A9L0J0P5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 20">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="20">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">GALNT6</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23956"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12788"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:53604"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52424"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="ChEBI" id="CHEBI:87075"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 20">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Golgi apparatus membrane</location>
            <topology evidence="2 20">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="20"/>
    <dbReference type="RefSeq" id="XP_044611671.1">
        <property type="nucleotide sequence ID" value="XM_044755736.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J0P5"/>
    <dbReference type="Ensembl" id="ENSEAST00005040065.1">
        <property type="protein sequence ID" value="ENSEASP00005045953.1"/>
        <property type="gene ID" value="ENSEASG00005006013.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835231"/>
    <dbReference type="KEGG" id="eai:106835231"/>
    <dbReference type="CTD" id="11226"/>
    <dbReference type="GeneTree" id="ENSGT00940000160845"/>
    <dbReference type="OMA" id="SWFLHNI"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000024">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000039">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF58">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 20" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="13 20" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10 20" id="KW-0430">Lectin</keyword>
    <keyword evidence="17 20" id="KW-0464">Manganese</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ricin B lectin" evidence="22">
        <location>
            <begin position="496"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="103"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="592"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="119"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00052209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005045953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="622" mass="71106" checksum="F76B2F4DA6EBAA6C" modified="2023-09-13" version="1">MRLLRRRHMPLRLAVVGSAFVFFLFILQRDVSSREQATEKPWLKSLVSQKDHVLDLMMGAVNNLRDSMPKLQIRAPEPQGTLVSTNQSCLPGFYTPAELKPFWERPPQDPNSPGADGKAFQKDKWTPQETQEKEEGYKKHCFNAFASDRISLQRALGPDTRPPECVDQKFRRCPPLPTTSVIIVFHNEAWSTLLRTVYSVLHTTPAILLREIILVDDASTDEYLKEQLEQYVKQLQVVRVVRQKERTGLITARLLGASVAQAEVLTFLDAHCECFHGWLEPLLARIAEDETAVVSPDIVTIDLNTFEFSKPVQRGRVHSRGNFDWSLSFGWEALPPHEKQRRKDETYPIKSPTFAGGLFSISKSYFEHIGTYDNQMEIWGGENVEMSFRVWQCGGQLEIIPCSVVGHVFRTKSPHTFPKGISVIARNQVRLAEVWMDGYKEIFYRRNMQAAKMAQEKSFGDISERLQLRERLHCHNFSWFLQNIYPEMFVPDLKPTFYGAIKNLGIDHCLDVGENNHGGKPLIMYTCHGLGGNQYFEYTTQRDLRHNIAKQLCLHASAGTLGLRSCHFTGKNSQVPKDEEWELTQDQLIRNSGSGTCLTSEDKKPAMASCNPSDPHQHWLFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J2X4</accession>
    <name>A0A9L0J2X4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc finger protein 32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ZNF32</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014691620.1">
        <property type="nucleotide sequence ID" value="XM_014836134.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014691623.1">
        <property type="nucleotide sequence ID" value="XM_014836137.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014691624.1">
        <property type="nucleotide sequence ID" value="XM_014836138.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070347806.1">
        <property type="nucleotide sequence ID" value="XM_070491705.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070347813.1">
        <property type="nucleotide sequence ID" value="XM_070491712.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070347826.1">
        <property type="nucleotide sequence ID" value="XM_070491725.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070347831.1">
        <property type="nucleotide sequence ID" value="XM_070491730.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070347838.1">
        <property type="nucleotide sequence ID" value="XM_070491737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J2X4"/>
    <dbReference type="Ensembl" id="ENSEAST00005078200.1">
        <property type="protein sequence ID" value="ENSEASP00005047494.1"/>
        <property type="gene ID" value="ENSEASG00005030797.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106827956"/>
    <dbReference type="KEGG" id="eai:106827956"/>
    <dbReference type="CTD" id="7580"/>
    <dbReference type="GeneTree" id="ENSGT00940000153582"/>
    <dbReference type="OMA" id="WDFQNAF"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000475">
        <property type="entry name" value="zinc finger protein 32 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000505">
        <property type="entry name" value="zinc finger protein 32 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001002">
        <property type="entry name" value="zinc finger protein 32 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24409">
        <property type="entry name" value="ZINC FINGER PROTEIN 142"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24409:SF331">
        <property type="entry name" value="ZINC FINGER PROTEIN 322A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="105"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005047494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="273" mass="30930" checksum="AE7F62D3565470B6" modified="2023-09-13" version="1">MFGFPAATLLDCHGRYAQNVAFFNVMTEAHHKYDHSDATGSSSWDFQNSFRKEKLEQKSPDSKTLPEDSPGVRQRVYECQECGKSFRQKGSLTLHERIHTGQKPFECTHCGKSFRAKGNLVTHQRIHTGEKPYQCKECGKSFSQRGSLAVHERLHTGQKPYECAICQRSFRNQSNLAVHRRVHSGEKPYRCDQCGKAFSQKGSLIVHIRVHTGLKPYACAQCRKSFHTRGNCLLHGKIHTGETPYLCGQCGKSFTQRGSLAVHQRSCSQRLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J4J4</accession>
    <name>A0A9L0J4J4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Dual specificity phosphatase 16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DUSP16</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
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                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
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    </dbReference>
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        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ALCOHOL DEHYDROGENASE 6"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="6">
        <location>
            <begin position="80"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005048291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="252" mass="26624" checksum="C239B5DA9DA558F0" modified="2023-09-13" version="1">MSDGTSRFTCKGKPVYHFGNTSTFSEYTVIKEISVTKIDAVAPLEKVCLISCGFSTGYGAAITTAKVTPGSSCAVFGLGGVGLSVIMGCKAAGAARIIGVDVNKDKFEKAKEVGATECINPQDFEKPIQEVLFDMTGAGVEFCFEAIGNPATVAAALASCHESHGVCVIVGLLAAGTQLNISGWLFFSGRSLKGSVFGGWKSRENVSKLVSDYMAKKFNLDPLITHTLNLDKINEAVELMKTGKCIRCVLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J4W4</accession>
    <name>A0A9L0J4W4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tyrosine-protein phosphatase non-receptor type</fullName>
            <ecNumber evidence="8">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PTPN21</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
            <location evidence="1 8">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0J4W4"/>
    <dbReference type="Ensembl" id="ENSEAST00005052349.1">
        <property type="protein sequence ID" value="ENSEASP00005048194.1"/>
        <property type="gene ID" value="ENSEASG00005020362.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155613"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13188">
        <property type="entry name" value="FERM_C_PTPN14_PTPN21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17192">
        <property type="entry name" value="FERM_F1_PTPN21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000014">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000149">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000039">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000030">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014392">
        <property type="entry name" value="PTP_non-rcpt_14/21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041782">
        <property type="entry name" value="PTPN14/21_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45706:SF3">
        <property type="entry name" value="TYROSINE-PROTEIN PHOSPHATASE NON-RECEPTOR TYPE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000934">
        <property type="entry name" value="Tyr-Ptase_nr14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6 8" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="13">
        <location>
            <begin position="23"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="11">
        <location>
            <begin position="896"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="12">
        <location>
            <begin position="1078"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="787"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="794"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="9">
        <location>
            <position position="1108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR000934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000934-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005048194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1174" mass="134109" checksum="FC889E08F77B96CB" modified="2023-09-13" version="1">MPLPFGLKLKRTRRYTVSSKSCLVARIQLLNNEFVEFTLSVESTGQESLEAVAQRLELREITYFSLWYYNKQNQRRWVDLEKPLKKQLDKYALEPTIYFGVVFYVPSVSQLQQEITRYQYYLQLKKDILEGNIPCTLEQAIQLAGLAVQADFGDFDQYESQDFLQKFALFPVGWLQDEKILEEATQKVALLHQKYRGLTAPDAEMLYMQEVERMDGYGEESYPAKDSQGSDISVGACLEGIFVKHKNGRPPVIFRWHDIANMSHNKSFFALELANKEETIQFQTEDMETAKYVWRLCVARHKFYRLNQCSLQTQTVTVNPIRRRSSSRMSLPKPQPYVMPPPPQLHYNGHYTEPYTSSQDNLFVTNQNGYYCHSQTSLDRAQIDLNGRIRNGSVYSAHSTNSLNNPQPYMQPSPMSSNPSITGSDVMRPDYVPSHRHSALIPPSYRPTPDYETVMKQLNRGMAHAERQSHSLRNLSIGNSYAYSRPDALVYSQPEIREHAHFTSPQSAHYPFNLNYSFHSQSPYPYPAERRPVVGAVSVPELTNVQLQAQEYPAPNIMKTQVYRPPPPYPYPRPANSTPDLSRHLYISSSNPDLITRRVHHSVQTFQEDSLPVAHSLQEVSEPLTSARHAQLQKRNSIEIAGLTHSFEGMRLKERTMSASAADVAPRAISAGSQPNVFIERTKQEETDEQESLRYGHKKSLSDATMLIHSSEEEEDFEEESKAQAALPLRAREPRGGYSQDLQPSYACGLLAGPLHILEPKSHVTETEKRVRDSSPVHMLVEAPRPRRDGLLTPSMSESDLTTSGRYRARRDSLKKRPVSDFLSGKKHIVEGLPPLGGMKKSRVDAKKIGPLKLAALNGLSLSRLPLPDEGNEVPTRATNDERCKILEQRLEQGMVFTEYERILKKRLVDGECSTARLPENAERNRFQDVLPYDDARVELVPTKENNTGYINASHIKVSVSGIEWDYIATQGPLQNTCQDFWQMVWEQGVAIIAMVTAEEEGGREKSFRYWPRLGSRHNTITYGRFKITTRFRTDSGCYATTGLKMKHLLTGQERTVWHLQYTDWPEHGCPEDLKGFLSYLEEIQSVRRHTNSTSEPKSPNPPLLVHCSAGVGRTGVVILSEIMIACLEHNEVLDIPRVLDMLRQQRMMMVQTLCQYTFVYRVLIQFLKSSRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J6U7</accession>
    <name>A0A9L0J6U7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106843465</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J6U7"/>
    <dbReference type="Ensembl" id="ENSEAST00005061149.1">
        <property type="protein sequence ID" value="ENSEASP00005045742.1"/>
        <property type="gene ID" value="ENSEASG00005027701.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286913"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000005">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="141"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="201"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="275"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005045742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="314" mass="35581" checksum="3A0A48D79D1C48D0" modified="2023-09-13" version="1">MWINNQSSLDDFILLGFSDRPWLETPLFVIFLVAYIFALFGNVSIILVSHLDPQLDSPMYFFLSNLSLLDLCYTTSTVPQMLVNLRGPEKTITYGGCVVQLYIFLALGSTECILLAAMAFDRYVAICKPLHYPVIMNQRRCIHMATGTWISGFANSLVQSTLTMLTPRCGQRVIDHFFCEVPAVLKLACTDTRINEAELNVLGALLLLVPLAFILGTYVFIARAVMRIRSAESRWKAFNTCASHLLVVSLFYFTAISMYVQPPSSYSRDRGKSMALFYGIVTPTLNPFIYTLRNKDVKAALRRTLTKEFWVKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J795</accession>
    <name>A0A9L0J795_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">L1 transposable element dsRBD-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J795"/>
    <dbReference type="Ensembl" id="ENSEAST00005039622.1">
        <property type="protein sequence ID" value="ENSEASP00005049039.1"/>
        <property type="gene ID" value="ENSEASG00005031286.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286982"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.250.20">
        <property type="entry name" value="L1 transposable element, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042566">
        <property type="entry name" value="L1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035300">
        <property type="entry name" value="L1_dsRBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004244">
        <property type="entry name" value="Transposase_22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11505">
        <property type="entry name" value="L1 TRANSPOSABLE ELEMENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17490">
        <property type="entry name" value="Tnp_22_dsRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="L1 transposable element dsRBD-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005049039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="80" mass="9495" checksum="E47BFAB96D8D072B" modified="2023-09-13" version="1">EWSDIFKALKDKNLQPRILYPARISFRYEGEIKSFPDKQKLREFVTKSPPLQEILKKALILEKRKKGERGHKPQTRETDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J959</accession>
    <name>A0A9L0J959_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Golgi associated, gamma adaptin ear containing, ARF binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">GGA2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
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                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="2">Early endosome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
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        <subcellularLocation>
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            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GGA protein family.</text>
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    <dbReference type="Ensembl" id="ENSEAST00005038362.1">
        <property type="protein sequence ID" value="ENSEASP00005045770.1"/>
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        <property type="component" value="Chromosome 14"/>
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        <property type="term" value="C:clathrin-coated vesicle"/>
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        <property type="term" value="F:phosphatidylinositol binding"/>
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        <property type="term" value="F:small GTPase binding"/>
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        <property type="term" value="F:ubiquitin binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:intracellular protein transport"/>
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        <property type="term" value="P:protein localization to cell surface"/>
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        <property type="project" value="Ensembl"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1230:FF:000001">
        <property type="entry name" value="ADP-ribosylation factor-binding protein GGA1 isoform 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.20.5.170:FF:000023">
        <property type="entry name" value="ADP-ribosylation factor-binding protein GGA3 isoform X1"/>
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        <property type="entry name" value="ADP-ribosylation factor-binding protein GGA3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.160:FF:000002">
        <property type="entry name" value="Golgi-associated, gamma adaptin ear containing, ARF binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR008152">
        <property type="entry name" value="Clathrin_a/b/g-adaptin_app_Ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013041">
        <property type="entry name" value="Clathrin_app_Ig-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
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        <property type="entry name" value="GAE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004152">
        <property type="entry name" value="GAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038425">
        <property type="entry name" value="GAT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027422">
        <property type="entry name" value="GGA1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041198">
        <property type="entry name" value="GGA_N-GAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45905:SF2">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-BINDING PROTEIN GGA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45905">
        <property type="entry name" value="GOLGI-LOCALIZED, GAMMA-ADAPTIN EAR CONTAINING, ARF BINDING PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF02883">
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="VHS" evidence="12">
        <location>
            <begin position="37"/>
            <end position="167"/>
        </location>
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    <feature type="domain" description="GAT" evidence="14">
        <location>
            <begin position="192"/>
            <end position="319"/>
        </location>
    </feature>
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        <location>
            <begin position="488"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="440"/>
            <end position="472"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JAE2</accession>
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        <recommendedName>
            <fullName evidence="2">Triadin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TRDN</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
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    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Guo"/>
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                <person name="Yang"/>
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                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
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                <person name="X. and Zhong"/>
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            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Contributes to the regulation of lumenal Ca2+ release via the sarcoplasmic reticulum calcium release channels RYR1 and RYR2, a key step in triggering skeletal and heart muscle contraction. Required for normal organization of the triad junction, where T-tubules and the sarcoplasmic reticulum terminal cisternae are in close contact. Required for normal skeletal muscle strength. Plays a role in excitation-contraction coupling in the heart and in regulating the rate of heart beats.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Sarcoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JAE2"/>
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        <property type="protein sequence ID" value="ENSEASP00005050139.1"/>
        <property type="gene ID" value="ENSEASG00005033610.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000049207"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
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    <accession>A0A9L0JE13</accession>
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        <recommendedName>
            <fullName evidence="6">C-type lectin domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="7" type="primary">LOC123278039</name>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="J."/>
                <person name="Li"/>
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                <person name="Liu"/>
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                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
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                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="Eosinophil_major_basic"/>
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        <property type="entry name" value="EMAJORBASICP"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0430">Lectin</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
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    <feature type="chain" id="PRO_5040113133" description="C-type lectin domain-containing protein" evidence="5">
        <location>
            <begin position="18"/>
            <end position="225"/>
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        <location>
            <begin position="112"/>
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        <location>
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            <taxon>Mammalia</taxon>
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            <taxon>Equidae</taxon>
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    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="1 3">Belongs to the 14-3-3 family.</text>
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            <taxon>Laurasiatheria</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
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    <comment type="cofactor">
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            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the tubulin family.</text>
    </comment>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0021987">
        <property type="term" value="P:cerebral cortex development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
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    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Beta-tubulin_BS"/>
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        <property type="entry name" value="Beta_tubulin"/>
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    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
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        <property type="entry name" value="Tubulin"/>
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        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
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        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00228">
        <property type="entry name" value="TUBULIN_B_AUTOREG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="14">
        <location>
            <begin position="47"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="15">
        <location>
            <begin position="246"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="422"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="429"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="445" mass="49907" checksum="93B3213EB2A9367B" modified="2023-09-13" version="1">MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAAGNKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVMPSPKVSDTVVEPYNATLSVHQLVENTDETYSIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMSATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATADEQGEFEEEEGEDEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JJ27</accession>
    <name>A0A9L0JJ27_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Monocarboxylate transporter 14</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Solute carrier family 16 member 14</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SLC16A14</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Proton-linked monocarboxylate transporter. May catalyze the transport of monocarboxylates across the plasma membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014691494.1">
        <property type="nucleotide sequence ID" value="XM_014836008.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JJ27"/>
    <dbReference type="Ensembl" id="ENSEAST00005079412.1">
        <property type="protein sequence ID" value="ENSEASP00005049200.1"/>
        <property type="gene ID" value="ENSEASG00005008423.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106827873"/>
    <dbReference type="KEGG" id="eai:106827873"/>
    <dbReference type="CTD" id="151473"/>
    <dbReference type="GeneTree" id="ENSGT00940000160895"/>
    <dbReference type="OMA" id="CVFISCC"/>
    <dbReference type="OrthoDB" id="2213137at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008028">
        <property type="term" value="F:monocarboxylic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17429">
        <property type="entry name" value="MFS_MCT14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:001028">
        <property type="entry name" value="Solute carrier family 16 member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:001049">
        <property type="entry name" value="Solute carrier family 16 member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF239">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0769">Symport</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="127"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="315"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="355"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="381"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="405"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="440"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="472"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="14">
        <location>
            <begin position="36"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="215"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00078720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005049200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="510" mass="56069" checksum="E39F99C48AB9C767" modified="2023-09-13" version="1">MYTSHEDIGYDFEDDPKDKKTLKPHPNIDGGWAWMMVLSSFFVHILIMGSQMALGVLNVEWLEEFHQSRGLTAWVSSLSMGITLIVGPFIGLFINTCGCRRTAIVGGLVNSLGWVLSAYAANVHYLFITFGVTAGFGSGMAYLPAVVMVGRYFQKRRALAQGLSTTGTGFGAFLMTVLLKYLCAEYGWRNAMFIQGAVSLNLCVCGALMRPLSPGKDGNDPGGRDPQVLPAPSTESIKSNGPVGRAEEKGDPPGNEETLSDLAAQACQEKAGLRKNMCALRVLKTVSQLTLRVRKGFRDWYSGYFGAASLFTNRMFVAFIFWALFAYSSFVIPFIHLPEIVNLYNLSEQNDVFPLTSIIAIVHIFGKVILGIVADLPCISVWNVFLMANFTLVLSIFILPLMHTYAGLAVICALIGFSSGYFSLMPVVTEDLVGIEHLANAYGIIICANGISALLGPPFAGWIYDITQKYDFSFYMCGLLYMVGILFLLIQPCLRFIEQSRKKYMDGAHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JJG6</accession>
    <name>A0A9L0JJG6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">SOSS complex subunit C</fullName>
            <shortName evidence="6">SOSS-C</shortName>
            <shortName evidence="6">SSB-interacting protein 1</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3 6">Sensor of single-strand DNA complex subunit C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5 6">Sensor of ssDNA subunit C</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4 6">Single-stranded DNA-binding protein-interacting protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">INIP</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. The SOSS complex associates with single-stranded DNA at DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. Required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs).</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the SOSS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the SOSS-C family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014714933.1">
        <property type="nucleotide sequence ID" value="XM_014859447.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JJG6"/>
    <dbReference type="SMR" id="A0A9L0JJG6"/>
    <dbReference type="Ensembl" id="ENSEAST00005069242.1">
        <property type="protein sequence ID" value="ENSEASP00005052498.1"/>
        <property type="gene ID" value="ENSEASG00005032850.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106842747"/>
    <dbReference type="KEGG" id="eai:106842747"/>
    <dbReference type="CTD" id="58493"/>
    <dbReference type="GeneTree" id="ENSGT00390000006366"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070876">
        <property type="term" value="C:SOSS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031821">
        <property type="entry name" value="SOSSC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31526">
        <property type="entry name" value="SOSS COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31526:SF2">
        <property type="entry name" value="SOSS COMPLEX SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15925">
        <property type="entry name" value="SOSSC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00033066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU369016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="104" mass="11425" checksum="87B2467920C58C1E" modified="2023-09-13" version="1">MAANSSGQGFQNKNRVAILAELDKEKRKLLMQNQSSTNHPGASIALSRPSLNKDFRDHAEQQHIAAQQKAALQHAHAHSSGYFITQDSAFGNLILPVLPRLDPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JJV3</accession>
    <name>A0A9L0JJV3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Zinc finger protein 521</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">ZNF521</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Transcription factor that can both act as an activator or a repressor depending on the context. Involved in BMP signaling and in the regulation of the immature compartment of the hematopoietic system. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved specification of B-cell lineage; this interaction preventing EBF1 to bind DNA and activate target genes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with EBF1. Interacts with SMAD1 and SMAD4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044630443.1">
        <property type="nucleotide sequence ID" value="XM_044774508.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070369386.1">
        <property type="nucleotide sequence ID" value="XM_070513285.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005048959.1">
        <property type="protein sequence ID" value="ENSEASP00005052663.1"/>
        <property type="gene ID" value="ENSEASG00005005129.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822948"/>
    <dbReference type="KEGG" id="eai:106822948"/>
    <dbReference type="CTD" id="25925"/>
    <dbReference type="GeneTree" id="ENSGT00940000159287"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048663">
        <property type="term" value="P:neuron fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000244">
        <property type="entry name" value="zinc finger protein 423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000483">
        <property type="entry name" value="Zinc finger protein 423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000107">
        <property type="entry name" value="Zinc finger protein 521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000143">
        <property type="entry name" value="Zinc finger protein 521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000167">
        <property type="entry name" value="Zinc finger protein 521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000261">
        <property type="entry name" value="Zinc finger protein 521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000998">
        <property type="entry name" value="Zinc finger protein 521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002441">
        <property type="entry name" value="Zinc finger protein 521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002477">
        <property type="entry name" value="Zinc finger protein 521"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:003011">
        <property type="entry name" value="zinc finger protein 521 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376:SF243">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24376">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="30"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="26"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="24"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0010">Activator</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="10" id="KW-0221">Differentiation</keyword>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="16" id="KW-0804">Transcription</keyword>
    <keyword evidence="13" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 21" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="23">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="23">
        <location>
            <begin position="146"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="23">
        <location>
            <begin position="174"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="23">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="23">
        <location>
            <begin position="246"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="23">
        <location>
            <begin position="281"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="23">
        <location>
            <begin position="310"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="23">
        <location>
            <begin position="437"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="23">
        <location>
            <begin position="477"/>
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        <name type="scientific">Equus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <text>a uridine in tRNA + S-adenosyl-L-methionine = a 3-[(3S)-3-amino-3-carboxypropyl]uridine in tRNA + S-methyl-5'-thioadenosine + H(+)</text>
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        <text evidence="5">Belongs to the TDD superfamily. DTWD2 family.</text>
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        <property type="term" value="F:tRNA-uridine aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="DTWD2/TAPT"/>
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    <dbReference type="PANTHER" id="PTHR21392">
        <property type="entry name" value="TRNA-URIDINE AMINOCARBOXYPROPYLTRANSFERASE 2"/>
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    <dbReference type="PANTHER" id="PTHR21392:SF0">
        <property type="entry name" value="TRNA-URIDINE AMINOCARBOXYPROPYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
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    <accession>A0A9L0JK99</accession>
    <name>A0A9L0JK99_EQUAS</name>
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        <recommendedName>
            <fullName evidence="10">Phosphodiesterase</fullName>
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        <name evidence="12" type="primary">PDE7A</name>
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        <name type="scientific">Equus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Y."/>
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                <person name="Q."/>
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                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.4.53"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_014701378.1">
        <property type="nucleotide sequence ID" value="XM_014845892.3"/>
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        <property type="protein sequence ID" value="ENSEASP00005053644.1"/>
        <property type="gene ID" value="ENSEASG00005001725.2"/>
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    <dbReference type="GeneID" id="106834319"/>
    <dbReference type="KEGG" id="eai:106834319"/>
    <dbReference type="CTD" id="5150"/>
    <dbReference type="GeneTree" id="ENSGT00940000157658"/>
    <dbReference type="OrthoDB" id="189220at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
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    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Phosphodiesterase"/>
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        <property type="entry name" value="HD/PDEase_dom"/>
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    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
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    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
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        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
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    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
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    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
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    <dbReference type="SMART" id="SM00471">
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        <property type="entry name" value="PDEASE_I_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0114">cAMP</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
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        <location>
            <begin position="136"/>
            <end position="458"/>
        </location>
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    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="212"/>
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    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="212"/>
            <end position="216"/>
        </location>
        <ligand>
            <name>AMP</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
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            <label>1</label>
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        <ligand>
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        <ligand>
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        <ligand>
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        </ligand>
    </feature>
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            <dbReference type="ARBA" id="ARBA00000621"/>
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    </evidence>
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    </evidence>
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                <person name="Li"/>
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                <person name="Zhang"/>
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                <person name="Jin"/>
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                <person name="Yue"/>
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                <person name="J."/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            <authorList>
                <consortium name="Ensembl"/>
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        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
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        <property type="entry name" value="KUNITZ-TYPE PROTEASE INHIBITOR 4"/>
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            <begin position="1"/>
            <end position="24"/>
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    <feature type="chain" id="PRO_5040417984" evidence="2">
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            <begin position="25"/>
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    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="3">
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            <begin position="41"/>
            <end position="91"/>
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    </feature>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50279"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="99" mass="11467" checksum="102A28762E660274" modified="2023-09-13" version="1">MKTAELRFLLGLFILFLLTTQLMGGVTRLTEMICGELKDPCQMDLKLGSCYEVHFRYFYNKTSKRCESFVYTGCDGNLNNYKLIIDCNIACVEAYQKHE</sequence>
</entry>
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    <accession>A0A9L0JNQ9</accession>
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        <recommendedName>
            <fullName evidence="10">Solute carrier family 25 member 45</fullName>
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    <organism evidence="14 15">
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044617806.1">
        <property type="nucleotide sequence ID" value="XM_044761871.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JNQ9"/>
    <dbReference type="Ensembl" id="ENSEAST00005057145.1">
        <property type="protein sequence ID" value="ENSEASP00005051602.1"/>
        <property type="gene ID" value="ENSEASG00005017943.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106833583"/>
    <dbReference type="CTD" id="283130"/>
    <dbReference type="GeneTree" id="ENSGT00940000161002"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Solute carrier family 25 member 45"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Mitochondrial carrier domain"/>
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    </dbReference>
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        <property type="entry name" value="MCP_dom_sf"/>
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        <property type="entry name" value="MCP_transmembrane"/>
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    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
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    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR45624:SF6">
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        <property type="entry name" value="Mito_carr"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
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        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11 13" id="KW-0472">Membrane</keyword>
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    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
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    <keyword evidence="5" id="KW-0677">Repeat</keyword>
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    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="121"/>
            <end position="144"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
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        <location>
            <begin position="66"/>
            <end position="149"/>
        </location>
    </feature>
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            <begin position="163"/>
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        <location>
            <begin position="265"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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            <taxon>Mammalia</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Liu"/>
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                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
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                <person name="Pan"/>
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                <person name="Zhang"/>
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                <person name="Jordana"/>
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                <person name="A."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the vexin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JXF7"/>
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        <property type="protein sequence ID" value="ENSEASP00005057163.1"/>
        <property type="gene ID" value="ENSEASG00005026057.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 12"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Vexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027900">
        <property type="entry name" value="Vexin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31520">
        <property type="entry name" value="VEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31520:SF1">
        <property type="entry name" value="VEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15505">
        <property type="entry name" value="Vexin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vexin" evidence="10">
        <location>
            <begin position="43"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="55"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="55"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF15505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005057163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="204" mass="22067" checksum="D323C0A4E9126B11" modified="2023-09-13" version="1">TMHQIYSCSDENIEVFTTVIPSKVSSPARRRVKSSQHLLTKNVVIESDLHTPRALELLPHRSDRRDAEGRRSGRLQTARPPGAQPAKTPARPVGISEPKSANLCGSRAYGKSLIPPVARISVKAPTVLEVAATGPENGAVLTRGSRHLKKMTEEYPTLPQGAEASLPLTGSASCGVPSILRKMWTRHKKKSEYVGATNSAFEAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JXU9</accession>
    <name>A0A9L0JXU9_EQUAS</name>
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        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CLDN1</name>
    </gene>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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            <fullName evidence="15">Dual specificity tyrosine-phosphorylation-regulated kinase 1A</fullName>
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            <ecNumber evidence="4">2.7.12.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Protein kinase minibrain homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">DYRK1A</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:10217"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.23" evidence="3"/>
    <dbReference type="EC" id="2.7.12.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044606601.1">
        <property type="nucleotide sequence ID" value="XM_044750666.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044606602.1">
        <property type="nucleotide sequence ID" value="XM_044750667.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044606603.1">
        <property type="nucleotide sequence ID" value="XM_044750668.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070344955.1">
        <property type="nucleotide sequence ID" value="XM_070488854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K5Y6"/>
    <dbReference type="SMR" id="A0A9L0K5Y6"/>
    <dbReference type="Ensembl" id="ENSEAST00005054532.1">
        <property type="protein sequence ID" value="ENSEASP00005057782.1"/>
        <property type="gene ID" value="ENSEASG00005006080.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831408"/>
    <dbReference type="KEGG" id="eai:106831408"/>
    <dbReference type="CTD" id="1859"/>
    <dbReference type="GeneTree" id="ENSGT00940000157408"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140857">
        <property type="term" value="F:histone H3T45 kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048156">
        <property type="term" value="F:tau protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043518">
        <property type="term" value="P:negative regulation of DNA damage response, signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031452">
        <property type="term" value="P:negative regulation of heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031115">
        <property type="term" value="P:negative regulation of microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048025">
        <property type="term" value="P:negative regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033120">
        <property type="term" value="P:positive regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902003">
        <property type="term" value="P:regulation of amyloid-beta formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902996">
        <property type="term" value="P:regulation of neurofibrillary tangle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14226">
        <property type="entry name" value="PKc_DYRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000087">
        <property type="entry name" value="Dual specificity tyrosine-phosphorylation-regulated kinase 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000264">
        <property type="entry name" value="dual specificity tyrosine-phosphorylation-regulated kinase 1B isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044131">
        <property type="entry name" value="PKc_DYR1A/1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF121">
        <property type="entry name" value="DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION-REGULATED KINASE 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="12" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="159"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="32"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="408"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="485"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="596"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="744"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="485"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="502"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="598"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="634"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="654"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="754"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00051680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005057782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="763" mass="85584" checksum="7C3A52A3CBB04FB5" modified="2023-09-13" version="1">MHTGGETSACKPSSVRLAPSFSFHAAGLQMAGQMPHSHQYSDRRQPNISDQQVSALSYSDQIQQPLTNQVMPDIVMLQRRMPQTFRDPATAPLRKLSVDLIKTYKHINEVYYAKKKRRHQQGQGDDSSHKKERKVYNDGYDDDNYDYIVKNGEKWMDRYEIDSLIGKGSFGQVVKAYDRVEQEWVAIKIIKNKKAFLNQAQIEVRLLELMNKHDTEMKYYIVHLKRHFMFRNHLCLVFEMLSYNLYDLLRNTNFRGVSLNLTRKFAQQMCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGMPYDLAIDMWSLGCILVEMHTGEPLFSGANEVDQMNKIVEVLGIPPAHILDQAPKARKFFEKLPDGTWNLKKTKDGKREYKPPGTRKLHNILGVETGGPGGRRAGESGHTVADYLKFKDLILRMLDYDPKTRIQPYYALQHSFFKKTADEGTNTSNSVSTSPAMEQSQSSGTTSSTSSSSGGSSGTSNSGRARSDPTHQHRHSGGHFTAAVQAMDCETHSPQVRQQFPAPLGWSGTEAPTQVTVETHPVQETTFHVAPQQNALHHHHGNSSHHHHHHHHHHHHHGQQALGNRTRPRVYNSPTNSSSTQDSMEVGHSHHSMTSLSSSTTSSSTSSSSTGNQGNQAYQNRPVAANTLDFGQNGAMDVNLTVYSNPRQETGIAGHPTYQFSANTGPAHYMTEGHLTMRQGADREESPMTGVCVQQSPVASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KDT8</accession>
    <name>A0A9L0KDT8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Iodothyronine deiodinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">DIO3</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="26">Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>3',5'-diiodo-L-thyronine + iodide + A + H(+) = 3,3',5'-triiodo-L-thyronine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83775"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:57261"/>
            <dbReference type="ChEBI" id="CHEBI:195762"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="23">
            <dbReference type="Rhea" id="RHEA:83777"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>3',5'-diiodothyronamine + iodide + A + H(+) = 3,3',5'-triiodothyronamine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83799"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:233342"/>
            <dbReference type="ChEBI" id="CHEBI:233343"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:83801"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>3'-iodo-L-thyronine + iodide + A + H(+) = 3,3'-diiodo-L-thyronine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83779"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:176514"/>
            <dbReference type="ChEBI" id="CHEBI:232695"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="22">
            <dbReference type="Rhea" id="RHEA:83781"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>3'-iodothyronamine + iodide + A + H(+) = 3,3'-diiodothyronamine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83815"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:233339"/>
            <dbReference type="ChEBI" id="CHEBI:233341"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:83817"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>3,3',5'-triiodo-L-thyronine + iodide + A + H(+) = L-thyroxine + AH2</text>
            <dbReference type="Rhea" id="RHEA:18897"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:57261"/>
            <dbReference type="ChEBI" id="CHEBI:58448"/>
            <dbReference type="EC" id="1.21.99.3"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:18899"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>3,3',5'-triiodothyronamine + iodide + A + H(+) = 3,3',5,5'-tetraiodothyronamine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83807"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:233343"/>
            <dbReference type="ChEBI" id="CHEBI:233344"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:83809"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>3,3'-diiodo-L-thyronine + iodide + A + H(+) = 3,3',5-triiodo-L-thyronine + AH2</text>
            <dbReference type="Rhea" id="RHEA:82571"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:176514"/>
            <dbReference type="ChEBI" id="CHEBI:533015"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="13">
            <dbReference type="Rhea" id="RHEA:82573"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>3,3'-diiodothyronamine + iodide + A + H(+) = 3,3',5-triiodothyronamine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83811"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:233341"/>
            <dbReference type="ChEBI" id="CHEBI:233426"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="18">
            <dbReference type="Rhea" id="RHEA:83813"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>3-iodo-L-thyronine + iodide + A + H(+) = 3,5-diiodo-L-thyronine + AH2</text>
            <dbReference type="Rhea" id="RHEA:82895"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:232626"/>
            <dbReference type="ChEBI" id="CHEBI:232627"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="24">
            <dbReference type="Rhea" id="RHEA:82897"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>3-iodothyronamine + iodide + A + H(+) = 3,5-diiodothyronamine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83823"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:231647"/>
            <dbReference type="ChEBI" id="CHEBI:233340"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="17">
            <dbReference type="Rhea" id="RHEA:83825"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-thyronine + iodide + A + H(+) = 3-iodo-L-thyronine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83771"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:232627"/>
            <dbReference type="ChEBI" id="CHEBI:233333"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:83773"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>thyronamine + iodide + A + H(+) = 3-iodothyronamine + AH2</text>
            <dbReference type="Rhea" id="RHEA:83819"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16382"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:231647"/>
            <dbReference type="ChEBI" id="CHEBI:233334"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="25">
            <dbReference type="Rhea" id="RHEA:83821"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Monomer. Homodimer. May undergo minor heretodimerization with DIO1 and DIO2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 26">Belongs to the iodothyronine deiodinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014710037.1">
        <property type="nucleotide sequence ID" value="XM_014854551.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0KDT8"/>
    <dbReference type="Ensembl" id="ENSEAST00005042953.1">
        <property type="protein sequence ID" value="ENSEASP00005063009.1"/>
        <property type="gene ID" value="ENSEASG00005035780.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106839648"/>
    <dbReference type="GeneTree" id="ENSGT00940000154482"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004800">
        <property type="term" value="F:thyroxine 5'-deiodinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033798">
        <property type="term" value="F:thyroxine 5-deiodinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042446">
        <property type="term" value="P:hormone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040018">
        <property type="term" value="P:positive regulation of multicellular organism growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097474">
        <property type="term" value="P:retinal cone cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046549">
        <property type="term" value="P:retinal cone cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042404">
        <property type="term" value="P:thyroid hormone catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000643">
        <property type="entry name" value="Iodothyronine_deiodinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008261">
        <property type="entry name" value="Iodothyronine_deiodinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027252">
        <property type="entry name" value="Iodothyronine_deiodinase_I/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11781">
        <property type="entry name" value="IODOTHYRONINE DEIODINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11781:SF4">
        <property type="entry name" value="THYROXINE 5-DEIODINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00837">
        <property type="entry name" value="T4_deiodinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001330">
        <property type="entry name" value="IOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500144">
        <property type="entry name" value="IODI_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01205">
        <property type="entry name" value="T4_DEIODINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 26" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 26" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 26" id="KW-0893">Thyroid hormones biosynthesis</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00065768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00093185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00093211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00093217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00093239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00093249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00093252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00093262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00093269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00093272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="RuleBase" id="RU000676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005063009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="302" mass="33632" checksum="18B3B68A5F916FE8" modified="2026-01-28" version="3">MPRQAAPRLVVGEGSGSQGALGAAATMLRSLLLHSLRLCAQTASCLVLFPRFLGTAFMLWLLDFLCIRKHFLSRQRRGGPEPEVELNCDGEEVPPDDPPICVSDDNRLCTLASLKAVWYGQKLDFFKQAHEGGPAPNSEVVLPDGFQNQHILDYARGNRPLVLNFGSCTCPPFMARMSAFRRLVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYIIPQHRSLEDRVSAARVLQQGAPGCSLVLDTMANSSSSAYGAYFERLYVIQSGTIMYQGGRGPDGYQVSELRTWLERYDEQLRGAQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KE03</accession>
    <name>A0A9L0KE03_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lipocalin/cytosolic fatty-acid binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC106845507</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KE03"/>
    <dbReference type="Ensembl" id="ENSEAST00005048605.1">
        <property type="protein sequence ID" value="ENSEASP00005059525.1"/>
        <property type="gene ID" value="ENSEASG00005027918.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000170466"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019841">
        <property type="term" value="F:retinol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002447">
        <property type="entry name" value="Blactoglobulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002345">
        <property type="entry name" value="Lipocalin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11430:SF117">
        <property type="entry name" value="GLYCODELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11430">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01172">
        <property type="entry name" value="BLCTOGLOBULN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00179">
        <property type="entry name" value="LIPOCALIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0494">Milk protein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0683">Retinol-binding</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="7">
        <location>
            <begin position="2"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005059525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="97" mass="10846" checksum="DA1902285D6E67C4" modified="2023-09-13" version="1">ANHVCVERNIVAQKTEDPAVFTVNYQGERKISVLDTDYAHYMFFCVGPPLPSAEHGTVCQYLARTQKVDEEVMEKFSRALQPLPGHVQIIQDPSGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KU83</accession>
    <name>A0A8C4KU83_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Spermine oxidase</fullName>
            <ecNumber evidence="13">1.5.3.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Polyamine oxidase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">SMOX</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Flavoenzyme which catalyzes the oxidation of spermine to spermidine. Can also use N(1)-acetylspermine and spermidine as substrates, with different affinity depending on the isoform (isozyme) and on the experimental conditions. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. May contribute to beta-alanine production via aldehyde dehydrogenase conversion of 3-amino-propanal.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>spermine + O2 + H2O = 3-aminopropanal + spermidine + H2O2</text>
            <dbReference type="Rhea" id="RHEA:25804"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:45725"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:58374"/>
            <dbReference type="EC" id="1.5.3.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amine and polyamine degradation; spermine degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.3.16" evidence="13"/>
    <dbReference type="RefSeq" id="XP_044603747.1">
        <property type="nucleotide sequence ID" value="XM_044747812.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070341716.1">
        <property type="nucleotide sequence ID" value="XM_070485615.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KU83"/>
    <dbReference type="Ensembl" id="ENSEAST00005001703.2">
        <property type="protein sequence ID" value="ENSEASP00005001515.2"/>
        <property type="gene ID" value="ENSEASG00005001238.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106847685"/>
    <dbReference type="CTD" id="54498"/>
    <dbReference type="GeneTree" id="ENSGT00940000157511"/>
    <dbReference type="OMA" id="CITGCHS"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052901">
        <property type="term" value="F:spermine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046208">
        <property type="term" value="P:spermine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000448">
        <property type="entry name" value="Putative polyamine oxidase 5 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000268">
        <property type="entry name" value="spermine oxidase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.660.10:FF:000003">
        <property type="entry name" value="spermine oxidase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.660.10:FF:000004">
        <property type="entry name" value="spermine oxidase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050281">
        <property type="entry name" value="Flavin_monoamine_oxidase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742">
        <property type="entry name" value="FLAVIN MONOAMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10742:SF416">
        <property type="entry name" value="SPERMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amine oxidase" evidence="17">
        <location>
            <begin position="34"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="Amine oxidase" evidence="17">
        <location>
            <begin position="313"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="271"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="274"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00075687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001515.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="555" mass="61686" checksum="ABA6CD10BD6C9C48" modified="2023-09-13" version="2">MQSCESSGDSADDPLSRGLRRRGQPRVVVIGAGLAGLAAAKALLEQGFTDVTVLEASSRIGGRVQSVKLGHATFELGATWIHGSHGNPIYHLAEANGLLEETTDGERSVGRISRYSKNGVACYLTNRGRRIPKDVVEEFSDLYNEVYNLTQEFFRHGKPVNAESQNSVGVFTREEVRNRIRDDPDDPEATKRLKLAMIQQYLKVESCESSSHSIDEVSLSAFGEWTEIPGAHHIIPSGFMRVVELLAEGIPAHVIQLGKPVRCVHWDQASARPRGPEIEPRGEGDHNHDTGEGSQGGEEPRGSGREEDEQWPVVVECEDCEVIPADHVIVTVSLGVLKRQHASFFRPGLPAEKVAAIHRLGIGTTDKIFLEFEEPFWGPECNSLQFVWEDEAESRTLTYPPELWYRKICGFDVLYPPERYGHVLSGWICGEEALVMEKCDDEAVAEICTEMLRQFTGNPNIPKPRRILRSAWGSDPYFRGSYSYTQVGSSGADVEKLAKPLPYTESSKTAPMQVLFSGEATHRKYYSTTHGALLSGQREAARLIEMYRDLFQQGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KW82</accession>
    <name>A0A8C4KW82_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">3-hydroxyisobutyrate dehydrogenase</fullName>
            <shortName evidence="10">HIBADH</shortName>
            <ecNumber evidence="10">1.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HIBADH</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-oxopropanoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17681"/>
            <dbReference type="ChEBI" id="CHEBI:11805"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57700"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Amino-acid degradation; L-valine degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HIBADH-related family. 3-hydroxyisobutyrate dehydrogenase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.31" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4KW82"/>
    <dbReference type="Ensembl" id="ENSEAST00005000068.2">
        <property type="protein sequence ID" value="ENSEASP00005000036.2"/>
        <property type="gene ID" value="ENSEASG00005000063.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155255"/>
    <dbReference type="UniPathway" id="UPA00362"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008442">
        <property type="term" value="F:3-hydroxyisobutyrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000006">
        <property type="entry name" value="3-hydroxyisobutyrate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000119">
        <property type="entry name" value="3-hydroxyisobutyrate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002204">
        <property type="entry name" value="3-OH-isobutyrate_DH-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011548">
        <property type="entry name" value="HIBADH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="HIBADH-like_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01692">
        <property type="entry name" value="HIBADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22981:SF7">
        <property type="entry name" value="3-HYDROXYISOBUTYRATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22981">
        <property type="entry name" value="3-HYDROXYISOBUTYRATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00895">
        <property type="entry name" value="3_HYDROXYISOBUT_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0101">Branched-chain amino acid catabolism</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7 10" id="KW-0520">NAD</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase NADP-binding" evidence="11">
        <location>
            <begin position="124"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyisobutyrate dehydrogenase-like NAD-binding" evidence="12">
        <location>
            <begin position="285"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU910714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000036.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="418" mass="44455" checksum="4608153F662EA877" modified="2023-09-13" version="2">MASSLRFRGAASGLRYWSRRQRPAAASLAAGKDLAPSSPVPPPAARFSRLTSEEIFSREALRLGRCLKPNILAGVKAWRLLPLPLAPLVLPSLPLLGIQMDDVQRPFRDLGRVCSRSMASKTPVGFIGLGNMGNPMAKNLMKHGYPLIIYDVFPDACKEFQDAGEQVVSSPADVAEKADRIITMLPTSINAIEAYSGANGILKKVKKGSLLIDSSTIDPAISKELAKEAEKMGAVFMDAPVSGGVGAARSGNLTFMVGGVEDEFAAAQELLGCMGSNVVYCGAVGTGQAAKICNNMLLAISMIGTAETMNLGIRLGLDPKLLAKILNMSSGRCWSSDSYNPVPGVMDGVPSANNYQGGFGTTLMAKDLGLAQDSATSTKSPILLGSQAHQIYRMMCAKGYSKKDFSSVFQFLREEETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KXP4</accession>
    <name>A0A8C4KXP4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Casein kinase I isoform alpha</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">CK1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">CSNK1A1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with the Axin complex. Interacts with TUT1, leading to TUT1 phosphorylation. Interacts with FAM83A, FAM83B, FAM83C, FAM83D, FAM83E, FAM83F, FAM83G and FAM83H (via DUF1669). Interaction with FAM83H recruits CSNK1A1 to keratin filaments.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_014708085.1">
        <property type="nucleotide sequence ID" value="XM_014852599.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KXP4"/>
    <dbReference type="SMR" id="A0A8C4KXP4"/>
    <dbReference type="Ensembl" id="ENSEAST00005004123.2">
        <property type="protein sequence ID" value="ENSEASP00005003758.2"/>
        <property type="gene ID" value="ENSEASG00005002855.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106838484"/>
    <dbReference type="CTD" id="1452"/>
    <dbReference type="GeneTree" id="ENSGT00940000153700"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030877">
        <property type="term" value="C:beta-catenin destruction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031670">
        <property type="term" value="P:cellular response to nutrient"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045104">
        <property type="term" value="P:intermediate filament cytoskeleton organization"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904262">
        <property type="term" value="P:negative regulation of TORC1 signaling"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044546">
        <property type="term" value="P:NLRP3 inflammasome complex assembly"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:1900227">
        <property type="term" value="P:positive regulation of NLRP3 inflammasome complex assembly"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035025">
        <property type="term" value="P:positive regulation of Rho protein signal transduction"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14128">
        <property type="entry name" value="STKc_CK1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000120">
        <property type="entry name" value="Casein kinase I isoform alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0137">Centromere</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8 17 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="17"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="337"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="337"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00071374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00082669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003758.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="365" mass="41937" checksum="11756FB15947D9F5" modified="2023-09-13" version="2">MASSSGSKAEFIVGGKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQKARHPQLLYESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDLFNFCSRRFTMKTVLMLADQMISRIEYVHTKNFIHRDIKPDNFLMGIGRHCNKCLESPVGKRKRSMTVSTSQDPSFSGLNQLFLIDFGLAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTMLKQKAAQQAASSSGQGQQAQTPTGKQTDKTKSNMKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KY70</accession>
    <name>A0A8C4KY70_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 569</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZNF569</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KY70"/>
    <dbReference type="Ensembl" id="ENSEAST00005003362.2">
        <property type="protein sequence ID" value="ENSEASP00005003076.2"/>
        <property type="gene ID" value="ENSEASG00005002372.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154650"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031981">
        <property type="term" value="C:nuclear lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004137">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000136">
        <property type="entry name" value="GLI family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000944">
        <property type="entry name" value="zinc finger protein 232 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002402">
        <property type="entry name" value="Zinc finger protein 347"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000023">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000281">
        <property type="entry name" value="Zinc finger protein 558 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000737">
        <property type="entry name" value="Zinc finger protein 565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001558">
        <property type="entry name" value="Zinc finger protein 569"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000394">
        <property type="entry name" value="Zinc finger protein 836"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF366">
        <property type="entry name" value="ZINC FINGER PROTEIN ZFP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="14">
        <location>
            <begin position="24"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="195"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="223"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="251"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="279"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="307"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="363"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="391"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="419"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="447"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="475"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="503"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="531"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="559"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="587"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="615"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="643"/>
            <end position="670"/>
        </location>
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        <location>
            <begin position="671"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003076.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="696" mass="80153" checksum="151BABC3D86567DF" modified="2023-09-13" version="2">MSAAFSGPVHSQKEEEMTESQGTVTFKDVAIDFTQEEWQQLDPAQRNLYRNVMLENYNNLITVGCPFTKPDVIFKLEQEEEPWVEEDVLRRHWPGEIWGIEEHQKIQDRLLTQLEDKFTKPLTEEKVNECHKKFANAFTLNSDFFPSSHNLYGYDLFGKCLEHNFVYCNNERILKYNEPVKSFGNSSSHLVVTPFKCNHCGKGFSQTLDLIRHLRIHTGEKPYECKKCKKAFSHKEKLIKHHKIHSREQSYECNECGKAFIKMSNLIRHQRIHTGEKPYACKECGKSFSQKSNLIDHEKIHTGEKPYECNECGKAFSQKQSLIAHQKVHTGEKPYACNECGKAFPRIASLALHMRSHTGEKPYKCDKCGKAFSQFSMLIIHVRIHTGEKPYECNECGKSFSQSSALTVHMRSHTGEKPYECKECRKAFSHKKNFITHQKIHTREKPYECNECGKAFIQMSNLVRHQRIHTGEKPYICKECGKAFSQKSNLIAHEKIHSGEKPYECNECGKAFSQKQNFITHQKVHTGEKPYDCNECGKAFSQIASLTLHLRSHTGEKPYECDKCGKAFSQCSLLNLHMRSHTGEKPYVCNECGKAFSQRTSLIVHMRGHTGEKPYECNKCGKAFSQSSSLTIHIRGHTGEKPFDCSKCGKAFSQISSLTLHMRKHTGEKPYHCNECGKAFSQKSHLVRHQRIHTHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KYS8</accession>
    <name>A0A8C4KYS8_EQUAS</name>
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        <recommendedName>
            <fullName evidence="20">Serine/threonine-protein kinase PLK</fullName>
            <ecNumber evidence="20">2.7.11.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Polo-like kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">PLK3</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 20">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="20">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.21" evidence="20"/>
    <dbReference type="RefSeq" id="XP_014683562.1">
        <property type="nucleotide sequence ID" value="XM_014828076.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KYS8"/>
    <dbReference type="Ensembl" id="ENSEAST00005004003.2">
        <property type="protein sequence ID" value="ENSEASP00005003646.2"/>
        <property type="gene ID" value="ENSEASG00005002638.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822748"/>
    <dbReference type="KEGG" id="eai:106822748"/>
    <dbReference type="CTD" id="1263"/>
    <dbReference type="GeneTree" id="ENSGT00940000159121"/>
    <dbReference type="OrthoDB" id="408964at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005795">
        <property type="term" value="C:Golgi stack"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090166">
        <property type="term" value="P:Golgi disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044819">
        <property type="term" value="P:mitotic G1/S transition checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904716">
        <property type="term" value="P:positive regulation of chaperone-mediated autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090316">
        <property type="term" value="P:positive regulation of intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010646">
        <property type="term" value="P:regulation of cell communication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023051">
        <property type="term" value="P:regulation of signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006970">
        <property type="term" value="P:response to osmotic stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009314">
        <property type="term" value="P:response to radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000302">
        <property type="term" value="P:response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd13118">
        <property type="entry name" value="POLO_box_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13117">
        <property type="entry name" value="POLO_box_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000189">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1120.30:FF:000001">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000091">
        <property type="entry name" value="Serine/threonine-protein kinase PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1120.30">
        <property type="entry name" value="POLO box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033701">
        <property type="entry name" value="POLO_box_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033695">
        <property type="entry name" value="POLO_box_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000959">
        <property type="entry name" value="POLO_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036947">
        <property type="entry name" value="POLO_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE PLK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24345:SF42">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PLK3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00659">
        <property type="entry name" value="POLO_box"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82615">
        <property type="entry name" value="Polo-box domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50078">
        <property type="entry name" value="POLO_BOX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="12 19 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="11 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 19 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 20" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6 20" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="22">
        <location>
            <begin position="62"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="23">
        <location>
            <begin position="464"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="POLO box" evidence="23">
        <location>
            <begin position="563"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="21">
        <location>
            <begin position="11"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003646.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="647" mass="71847" checksum="8DBBC4136433DC8C" modified="2023-09-13" version="2">MEPAAGFLSPRPFPRAAAPPAPPAGPGPPPSAMPGPELEMLASPPAPDSGRLITDPCSGRTYFKGRLLGKGGFARCYEATDRETGNAYAVKVISQSRITKPHQREKILNEIELHRGLQHRHIVRFSHHFEDADNIYIFLELCSRKSLAHIWKARHTLLEPEVRYYLRQILSGLKYLHQKGILHRDLKLGNFFITENMELKMGDFGLATRLEPPEQRKKTICGTPNYVAPEVLQRQGHGPEADVWSLGCVMYTLLCGSPPFETVDLKETYRCIKQVHYTLPASLSLPARQLLAAILRASPQDRPSIDQILRHDFFTKGYTPDRLPVSSCVTVPDLIPLNAAKILFVKVTKSLFGRKKNKSKNHPEERDEVSCLVNGLTRTSIGHQDARPEAPAASGPAPVSLVETALEDSSPRGTLASSGDGFEEGLTVATVVESALCALRNCLAFMPPAEQNPAPLAQPEPLVWVSKWVDYSNKFGFGYQLSSRRVAVLFNNGTHMALSANRKTVHYNPTSTKHFSFSVGAVPRALQPQLGVLRYFASYMEQRLMKGGDLPSVEEVEVPVPPLLLQWVKTEQALLMLFSDGTVQVNFYGDHTKLILSGWEPLLVTFVARNRSACTYLASHLRQLGCSPDLRQRLGYALRLLRDRCPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KYV8</accession>
    <name>A0A8C4KYV8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein FAM183A</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="19">Activated by DAG and phorbol esters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 19">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.13" evidence="19"/>
    <dbReference type="RefSeq" id="XP_044615230.1">
        <property type="nucleotide sequence ID" value="XM_044759295.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L3M6"/>
    <dbReference type="Ensembl" id="ENSEAST00005005963.2">
        <property type="protein sequence ID" value="ENSEASP00005005457.2"/>
        <property type="gene ID" value="ENSEASG00005004020.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106845140"/>
    <dbReference type="KEGG" id="eai:106845140"/>
    <dbReference type="CTD" id="25865"/>
    <dbReference type="GeneTree" id="ENSGT00950000183024"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004697">
        <property type="term" value="F:diacylglycerol-dependent serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005080">
        <property type="term" value="F:protein kinase C binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106310">
        <property type="term" value="F:protein serine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035924">
        <property type="term" value="P:cellular response to vascular endothelial growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045766">
        <property type="term" value="P:positive regulation of angiogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043536">
        <property type="term" value="P:positive regulation of blood vessel endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045785">
        <property type="term" value="P:positive regulation of cell adhesion"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000573">
        <property type="term" value="P:positive regulation of DNA biosynthetic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001028">
        <property type="term" value="P:positive regulation of endothelial cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001938">
        <property type="term" value="P:positive regulation of endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045743">
        <property type="term" value="P:positive regulation of fibroblast growth factor receptor signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032743">
        <property type="term" value="P:positive regulation of interleukin-2 production"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032757">
        <property type="term" value="P:positive regulation of interleukin-8 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050862">
        <property type="term" value="P:positive regulation of T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030949">
        <property type="term" value="P:positive regulation of vascular endothelial growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070232">
        <property type="term" value="P:regulation of T cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048010">
        <property type="term" value="P:vascular endothelial growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd20840">
        <property type="entry name" value="C1_PKD2_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20843">
        <property type="entry name" value="C1_PKD2_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01239">
        <property type="entry name" value="PH_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14082">
        <property type="entry name" value="STKc_PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000151">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000056">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000137">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000007">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000019">
        <property type="entry name" value="Serine/threonine-protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015727">
        <property type="entry name" value="Protein_Kinase_C_mu-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057764">
        <property type="entry name" value="Ubiquitin_PRKD1-3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968">
        <property type="entry name" value="PROTEIN KINASE C, MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22968:SF12">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25525">
        <property type="entry name" value="Ubiquitin_PRKD1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000552">
        <property type="entry name" value="PKC_mu_nu_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 19 21" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="16 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 19 21" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="26">
        <location>
            <begin position="138"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="26">
        <location>
            <begin position="264"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="24">
        <location>
            <begin position="397"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="25">
        <location>
            <begin position="551"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="343"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="844"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="20">
        <location>
            <position position="674"/>
        </location>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <begin position="557"/>
            <end position="565"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21 22">
        <location>
            <position position="580"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR000552"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR000552-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRSR" id="PIRSR000552-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005457.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="878" mass="96906" checksum="05D2B0AA015BC66A" modified="2023-09-13" version="2">MAAAPSHSAGLPGSPGPGSPPLIGGLELQSPPPLLPQVPAPGPGVSFHIQIGLTREFVLLPTASELAHVKQLACSIVDQKFPECGFYGLYDKILLFKHDPTSANLLQLVRSAGDIQEGDLVEVVLSASATFEDFQIRPHALTVHSYRAPAFCDHCGEMLFGLVRQGLKCDGCGLNYHKRCAFSIPNNCSGARKRRLSSTSLASGHSVRLGTSESLPCTAEELNRSTTELLPRRPHSSSSSSSASSYTGRPIELDKMLLSKVKVPHTFLIHSYTRPTVCQACKKLLKGLFRQGLQCKDCKFNCHKRCATRVPNDCLGEALINGDVPMEEATDFSEADKSALMDEPDDAGVIPGSHSESALHASEEDEGEGAKAQSSLGYIPLMRVVQSVRHTTRKSSTTLREGWMVHYSNKDTLRKRHYWRLDCKCITLFQNNTTNRYYKEIPLSEILSVEPSQNFSLVPPGTNPHCFEIVTANATYFVGETPGGVPGGPSVQGAEAARGWETAIRQALMPVILQDAPSPPGHTPHRQASLSISVSNSEIQENVDIATVYQIFPDEVLGSGQFGVVYGGKHRKTGRDVAVKVIDKLRFPTKQESQLRNEVAILQSLRHPGIVNLECMFETPEKVFVVMEKLHGDMLEMILSSEKGRLPERLTKFLITQILVALRHLHFKNIVHCDLKPENVLLASADPFPQVKLCDFGFARIIGEKSFRRSVVGTPAYLAPEVLLNQGYNRSLDMWSVGVIMYVSLSGTFPFNEDEDINDQIQNAAFMYPACPWSCISVGAIDLINNLLQVKMRKRYSVDKSLSHSWLQEYQTWLDLRELERKMGERYITHESDDTRWEQFVAEHPLPGPGLPADGELGGALPPQDHEMQGLAERISVL</sequence>
</entry>
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    <accession>A0A8C4L4G1</accession>
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            <taxon>Metazoa</taxon>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_044609038.1">
        <property type="nucleotide sequence ID" value="XM_044753103.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L4G1"/>
    <dbReference type="Ensembl" id="ENSEAST00005006752.2">
        <property type="protein sequence ID" value="ENSEASP00005006169.2"/>
        <property type="gene ID" value="ENSEASG00005004571.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106846603"/>
    <dbReference type="KEGG" id="eai:106846603"/>
    <dbReference type="CTD" id="57139"/>
    <dbReference type="GeneTree" id="ENSGT00940000161935"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17212">
        <property type="entry name" value="RA_RGL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000159">
        <property type="entry name" value="RA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030750">
        <property type="entry name" value="RGL3_RA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF220">
        <property type="entry name" value="RAL GUANINE NUCLEOTIDE DISSOCIATION STIMULATOR-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00788">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00314">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50200">
        <property type="entry name" value="RA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="6">
        <location>
            <begin position="66"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="4">
        <location>
            <begin position="249"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-associating" evidence="5">
        <location>
            <begin position="615"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="201"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="398"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="497"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="209"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="399"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="544"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="556"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006169.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="712" mass="77509" checksum="4E27081BF0F3201B" modified="2023-09-13" version="2">MERTAGRELALAPLQDWGQETEDGAVYSVSLRRQRSQRWSPGQGPGGGEVPSPAACDAFLHYRTSKVRALRAARLERLVQELVSGDRAQDPGFVPAFLATHRAFVPTARVLALLLPPPPPPLPPGVEIKKTEGRDLSFNQNLRAVVSVLGSWLRDHPQDFRDPPAHSDLSSVRTFLGWAAPLGAEAREAEKLLGEFLEEAEHAQEEEAQPQARAGPPGAAQTPCAGCPEGCSQEEEGLVQEGPELLDFGVDEVAEQLTLLDVELFSRVRPCECLGSVWSQRDRPGAAGVAPTVRATVTQFNTVTGCVLGSVLGPPGLAAPQRAQRLEKWIRIAQRCRELRNFSSLRAILSALQSNPIYRLKRSWGAVSREPLSTFRKLSQIFSDENNHLSCREILSQEEATEGPQEEDTPPGSLPSKLPPGPVPYLGTFLTDLIMLDTALPDMLEGDLINFEKRRKEWEILARIQQLQRRCRSYCLSPHPPVLAALRAQRQLSEEQSYHVSRVTEPPAASCPSSPRVRRRISLTKRLSAKLSREKGPSPGGSPGDPSSPTSSLSPGSPPSSPRSKDPPPGSTPASPGPQVPSTKLPLSPDLPGPRPLALSLSGPCIPLPGLQGSEARVIRVSIDNDHGNLYRSILLTSQDKAPSVVQRALQKHNVAQPWAHDYQLFQVLPGDRELLIPDNANVFYAMSPAAPGDFMLRRKEGARHTNSVSPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L5G0</accession>
    <name>A0A8C4L5G0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Leucine-rich repeat and fibronectin type-III domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LRFN2</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the LRFN family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L5G0"/>
    <dbReference type="Ensembl" id="ENSEAST00005004189.2">
        <property type="protein sequence ID" value="ENSEASP00005003820.1"/>
        <property type="gene ID" value="ENSEASG00005002899.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156417"/>
    <dbReference type="OMA" id="ELMDFSA"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
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        <property type="entry name" value="SYNAPTIC ADHESION-LIKE MOLECULE SALM"/>
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    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="4" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 19" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <keyword evidence="5 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034908577" description="Leucine-rich repeat and fibronectin type-III domain-containing protein 2" evidence="19">
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            <begin position="21"/>
            <end position="793"/>
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            <begin position="529"/>
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            <begin position="289"/>
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            <begin position="421"/>
            <end position="518"/>
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            <begin position="383"/>
            <end position="424"/>
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    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="617"/>
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        <location>
            <begin position="669"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="406"/>
            <end position="416"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="617"/>
            <end position="632"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
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    <evidence type="ECO:0000256" key="2">
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            <dbReference type="ARBA" id="ARBA00004479"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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        <source>
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    </evidence>
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    <evidence type="ECO:0000259" key="21">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
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    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="793" mass="84953" checksum="E97D0BF5748D8F26" modified="2022-01-19" version="1">METLLGGLLAFGMAFAVVDACPKYCVCQNLSESLGTLCPSKGLLFVPPDIDRRTVELRLGGNFIIHIGRQDFANMTGLVDLTLSRNTISHIQPFSFLDLESLRSLHLDSNRLPSLGEDTLRGLVNLQHLIVNNNQLGGIADEAFEDFLLTLEDLDLSYNNLQGLPWGSVRRMVNLHQLSLDHNLLDHIAEGTFADLQKLARLDLTSNRLQKLPPDPIFARSQASALTATPFAPPLSFSFGGNPLHCNCELLWLRRLERDDDLETCGSPGGLKGRYFWHVREEEFVCEPPLITQHTHKLLVLEGQAATLKCKAIGDPSPLIHWVAPDDRLVGNSSRTAVYDNGTLDILITTSQDSGSFTCIAANAAGEATAMVELSIVQLPHLSNSTSRTAPPKSRLSDITGSSKTGRGGGGSGGGEPPKSPPERAVLVSDVTTTSALVKWSVSKSAPRVKMYQLQYNCSDDEVLIYRMIPAANKAFVVNNLVSGTGYDLCVLAMWDDTATTLTATNIVGCAQFFTKADYPQCQSMHSQLLGGTMILVIGGIIVATLLVFIVILMVRYKVCNQEAPGKMVATTAVSNMHSQTNGAQLPALSSAPSGAPVQAPPKVVVRNELVQFSASLARGSDSSSSSSLGSGEAAGLGHGPWRLPPPASRPKPNLDRLMGAFASLDLKSQRKEELLDSRTPGGRGSGTSARGHQSDREPLLGPPTARARSLLPLPLEGKAKRSHSFDMGDFAAAAAAAGAVTAGGYSPPRRVSNIWTKRSLSVNGMLLPFEESDLVGARGTFGSSEWVMESTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L993</accession>
    <name>A0A8C4L993_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Putative ATP-dependent RNA helicase DHX57</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">DEAH box protein 57</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">DHX57</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Probable ATP-binding RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 23">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="Ensembl" id="ENSEAST00005008273.2">
        <property type="protein sequence ID" value="ENSEASP00005007585.2"/>
        <property type="gene ID" value="ENSEASG00005005539.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156883"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17985">
        <property type="entry name" value="DEXHc_DHX57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd23825">
        <property type="entry name" value="RWD_DHX57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14317">
        <property type="entry name" value="UBA_DHX57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000325">
        <property type="entry name" value="ATP-dependent RNA helicase DHX29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000284">
        <property type="entry name" value="probable ATP-dependent RNA helicase YTHDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000002">
        <property type="entry name" value="Putative ATP-dependent RNA helicase DHX36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.10:FF:000038">
        <property type="entry name" value="putative ATP-dependent RNA helicase DHX57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.10">
        <property type="entry name" value="Zinc finger, CCCH-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042615">
        <property type="entry name" value="DHX57_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059023">
        <property type="entry name" value="RNA_hel_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006575">
        <property type="entry name" value="RWD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF145">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX57-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26026">
        <property type="entry name" value="RNA_hel_CTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05773">
        <property type="entry name" value="RWD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBA" evidence="17">
        <location>
            <begin position="170"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="18">
        <location>
            <begin position="295"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="551"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="827"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="15">
        <location>
            <begin position="295"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="117"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="453"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="80"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="469"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
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            <dbReference type="ARBA" id="ARBA00022806"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00057709"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
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            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="17">
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            <dbReference type="PROSITE" id="PS50030"/>
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            <dbReference type="PROSITE" id="PS50103"/>
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    <evidence type="ECO:0000259" key="20">
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        </source>
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    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007585.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1383" mass="155250" checksum="F6D78DE8748639A0" modified="2023-09-13" version="2">MSSSVRRKGKPGKGGGKGSSRGGRGGRSHASKSHGGGSGGGGGGGGGNRKASSRIWDDGDDFCIFSESRRSSRPSNSSIRKGEARPKWKPEAKVPQTLHMTSENQEKVKALLRDLQEQDADAGSERGISGEEEDDEPDYDDEQYWSAGREASLVPDADPLEYAGLGLVEPYNPEFAVSTFAVQKLSRYGFNTERCQAALRICDGDVGASLEHLLTQCFSETFGERVEISEAVARISLDECVEQRQEEAFALKSICGEKFIERIQNRVWTIGLELEYLTSKFCKSRPKESTKNVQETSLEVCKFYLKGNCKFGSKCKFKHEVPPNQIVGRAERSVDDSHLNANEDASFLYELEIRFSKDHKYPYQAPLVAFYSTNENLPLACRLHISEFLYGKALIFAETSEPVVYSLITLLEEESEIVKLLTNTHHKYSVPPVNPLPVSSATRVNNPVCRKPVIPNNSFGSNQTPEVEKESEPEEETDEDEGPAPVIVENESYVNLKKKISKRYDWQAKSVHAENAKICKQFRIKQASRQFQSILQERQSLPAWEERETILKLLSQHQVLVISGMTGCGKTTQIPQFILDDSLNGPPEKVANIICTQPRRISAISVAERVAKERAERVGLTVGYQIRLESVKSSATRLLYCTTGVLLRRLEGDTALQGITHIIVDEVHERTEESDFLLLVLKDIVLQRPTLQVILMSATLNAELFSEYFNSCPVITIPGRTFPVDQFFLEDAIAVTRYVLPDGSPYMRSMKQMSKEKLKARHNRTAFEEVEEDLRLSLHLQDQDSVKDAVPDQQLDFKQLLARYKGVSKSVIKTMSIMDFEKVNLELIEALLEWIVDGKHSYPPGAILVFLPGLAEIKMLYEQLQSNSLFNNRRSNRCVIHPLHSSLSSEEQQAVFVKPPVGVTKIIISTNIAETSITIDDVVYVIDSGKMKEKRYDASKGMESLEDTFVSQANALQRKGRAGRVASGVCFHLFTSHHFNHQLLKQQLPEIQRVPLEQLCLRIKILEMFSTHNLQSVFSRLIEPPHIESLRASKIRLRDLGALTPDEKLTPLGYHLASLPVDVRIGKLMLFGSIFRCLDPALTIAASLAFKSPFVSPWDKKEEANQKKLEFAFANSDYLALLRAYKGWQLSMKEGMRASYNYCRQNFLSGRVLQEMASLKRQFTELLSDIGFVKEGLRARDIEKRAQGGDGILETTGEEANSNAENPKLISAMLCAALYPNVVQVKSPEGKFQKTSTGAVRMQPKSDELKFVTKNDGYVHIHPSSVNYQVRHFDSPYLVYHEKIKTSRVFIRDCSMVSVYPLVLFGGGQVNVQLQRGEFIVSLDDGWIRFAAASHQVAELVKELRCELDQLLQDKIKNPSIDLCTCPRGSRIISMIVKLVTTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LCD3</accession>
    <name>A0A8C4LCD3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Lipid-binding serum glycoprotein N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BPI/LBP/Plunc superfamily. Plunc family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LCD3"/>
    <dbReference type="Ensembl" id="ENSEAST00005006462.2">
        <property type="protein sequence ID" value="ENSEASP00005005908.2"/>
        <property type="gene ID" value="ENSEASG00005004350.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000263546"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019731">
        <property type="term" value="P:antibacterial humoral response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061844">
        <property type="term" value="P:antimicrobial humoral immune response mediated by antimicrobial peptide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002395">
        <property type="term" value="P:immune response in nasopharyngeal-associated lymphoid tissue"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900229">
        <property type="term" value="P:negative regulation of single-species biofilm formation in or on host organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043129">
        <property type="term" value="P:surfactant homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051902">
        <property type="entry name" value="BPI_fold-superfamily_member"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017942">
        <property type="entry name" value="Lipid-bd_serum_glycop_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47015">
        <property type="entry name" value="BPI FOLD-CONTAINING FAMILY A MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47015:SF1">
        <property type="entry name" value="BPI FOLD-CONTAINING FAMILY A MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01273">
        <property type="entry name" value="LBP_BPI_CETP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0044">Antibiotic</keyword>
    <keyword evidence="4" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040115233" description="Lipid-binding serum glycoprotein N-terminal domain-containing protein" evidence="11">
        <location>
            <begin position="21"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Lipid-binding serum glycoprotein N-terminal" evidence="12">
        <location>
            <begin position="83"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005908.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="258" mass="27699" checksum="A1B8672FA0042E35" modified="2023-09-13" version="2">MFEIGGLIVFCGLLAHKAAPLEVLSSALNETLPSLLKQTLPSPLNQTLPSPLNQTLHSPLNQTLPSPINQTLPSPLNQTLDGLLSEGLLGRLKKLPLSSTLKAEGVTSRAVFGGLLGKIATVIPFVNIIDVKITHPRLLDLDLLKSPDGSRFYVTIPLDMILNVNVPLVGSLLKGGVKLNITAELLTAQGEQKNIPLVLDSCTHPAGSQRGLCITPWLFPFRFPLIPFQRLFKSLSRILPGLVQGKVSGLSGSKRPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LCF9</accession>
    <name>A0A8C4LCF9_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Solute carrier family 37 member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SLC37A4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Organophosphate:Pi antiporter (OPA) (TC 2.A.1.4) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044608667.1">
        <property type="nucleotide sequence ID" value="XM_044752732.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070346227.1">
        <property type="nucleotide sequence ID" value="XM_070490126.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070346228.1">
        <property type="nucleotide sequence ID" value="XM_070490127.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070346229.1">
        <property type="nucleotide sequence ID" value="XM_070490128.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LCF9"/>
    <dbReference type="Ensembl" id="ENSEAST00005007292.2">
        <property type="protein sequence ID" value="ENSEASP00005006670.2"/>
        <property type="gene ID" value="ENSEASG00005004926.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106840292"/>
    <dbReference type="CTD" id="2542"/>
    <dbReference type="GeneTree" id="ENSGT00730000111086"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061513">
        <property type="term" value="F:glucose 6-phosphate:phosphate antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17343">
        <property type="entry name" value="MFS_SLC37A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000112">
        <property type="entry name" value="glucose-6-phosphate exchanger SLC37A4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000111">
        <property type="entry name" value="Solute carrier family 37 member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051337">
        <property type="entry name" value="OPA_Antiporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021159">
        <property type="entry name" value="Sugar-P_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000849">
        <property type="entry name" value="Sugar_P_transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00881">
        <property type="entry name" value="2A0104"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43826">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE EXCHANGER SLC37A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43826:SF3">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE EXCHANGER SLC37A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002808">
        <property type="entry name" value="Hexose_phosphate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00942">
        <property type="entry name" value="GLPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="225"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="329"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="365"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="396"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="13"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006670.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="429" mass="46342" checksum="D1B89DA88B29BBB7" modified="2023-09-13" version="2">MAAQGYSYYRTVIFLAMFGGYSLYYFNRKTFSFVMPSVVEEIPLDKDDLGLITSSQSAAYAISKFVSGVLSDQMSARWLFSSGLLLVGLVNIVFSWSSTVPVFAALWFLNGLAQGLGWPPCGKVLRKWFEPSQFGTWWAILSTSMNLAGGLGPILATILAQSYSWRSTLALSGALCVVVSFLCLLLIHNEPADVGLRNLDPTPSKGKKGSLKEESTLRELLLSPYLWVLSTGYLVVFGVKTCCTDWGQFFLIQEKGQSALVGSSYMSALEVGGLIGSIAAGYLSDRAMAKAGLSIYGNPRHGLLLFMMAGMTVSMYLFRVTVTSDSPKLWILVLGAVFGFSSYGPIALFGVIANESAPPNLCGTSHAVVGLMANVGGFLAGLPFSTIAKHYSWSTAFWVAEVICAASTAAFFLLRNIRTKMGRVPKKAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LCL7</accession>
    <name>A0A8C4LCL7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cell growth-regulating nucleolar protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LYAR</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="9">Interacts with PRMT5; this interaction is direct. Interacts with GNL2 and RPL23A. Interacts with nucleolin/NCL; this interaction is direct. Interacts with phosphorylated IRF3; this interaction impairs IRF3 DNA-binding activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Photoreceptor outer segment</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014719825.1">
        <property type="nucleotide sequence ID" value="XM_014864339.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070362338.1">
        <property type="nucleotide sequence ID" value="XM_070506237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LCL7"/>
    <dbReference type="Ensembl" id="ENSEAST00005007901.2">
        <property type="protein sequence ID" value="ENSEASP00005007240.1"/>
        <property type="gene ID" value="ENSEASG00005005311.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106845882"/>
    <dbReference type="KEGG" id="eai:106845882"/>
    <dbReference type="CTD" id="55646"/>
    <dbReference type="GeneTree" id="ENSGT00390000003477"/>
    <dbReference type="OMA" id="QNWIKNS"/>
    <dbReference type="OrthoDB" id="21474at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
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    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:nucleoplasm"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:DNA-binding transcription factor binding"/>
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        <property type="term" value="F:identical protein binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:transcription regulator inhibitor activity"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:erythrocyte development"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:negative regulation of innate immune response"/>
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        <property type="term" value="P:positive regulation of phagocytosis"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
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        <property type="term" value="P:rRNA processing"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="FunFam" id="1.10.10.2100:FF:000002">
        <property type="entry name" value="cell growth-regulating nucleolar protein-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.1490.490:FF:000001">
        <property type="entry name" value="cell growth-regulating nucleolar protein-like"/>
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    <dbReference type="InterPro" id="IPR039999">
        <property type="entry name" value="LYAR"/>
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    <dbReference type="InterPro" id="IPR058719">
        <property type="entry name" value="WHD_LYAR"/>
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    <dbReference type="InterPro" id="IPR014898">
        <property type="entry name" value="Znf_C2H2_LYAR"/>
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    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
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    <dbReference type="PANTHER" id="PTHR13100:SF10">
        <property type="entry name" value="CELL GROWTH-REGULATING NUCLEOLAR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13100">
        <property type="entry name" value="CELL GROWTH-REGULATING NUCLEOLAR PROTEIN LYAR"/>
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        <property type="entry name" value="WHD_LYAR"/>
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    <dbReference type="Pfam" id="PF08790">
        <property type="entry name" value="zf-LYAR"/>
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    <dbReference type="SUPFAM" id="SSF57667">
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    <dbReference type="PROSITE" id="PS51804">
        <property type="entry name" value="ZF_C2HC_LYAR"/>
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    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger C2H2 LYAR-type" evidence="13">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="Cell growth-regulating nucleolar protein-like winged helix" evidence="14">
        <location>
            <begin position="315"/>
            <end position="387"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="142"/>
            <end position="317"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
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            <begin position="203"/>
            <end position="216"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
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            <begin position="300"/>
            <end position="314"/>
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            <dbReference type="Pfam" id="PF25879"/>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="388" mass="44078" checksum="FD883BE32753ABDB" modified="2022-01-19" version="1">MVFFTCNACGESVKKVQVEKHVAICRNCECLSCIDCGKDFWGDDYKNHVKCISEDQKYGGKGYEGKTHKGDIKQQAWIQKINELIKRPNVSPKVRELLEQISAFDNVPRKKAKFQNWMKNSLKVHNESVLEQVWNIFSEASSTEPVSKERDQQPLNPVADPHAEISAKVPCSKANDTTEEQAKVKKNKRERKEERQKKRKKEKKELRLENHQENSKSQKPKKRKMGREAEQEAGGEDSPKASGSAGKPSQRKRSKGLCAEDGAVGGGVDEEEAKTGPGKRKRKHSEIEAESKKKKIKFPGHSEGEEPENHEASAKGKFNWKGTIKAVLKQAPDNEIAIKKLRKKVLAQYYAVTNEHHKSEEELLVIFNKKISKNPTFKLLKDKVKLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LCV3</accession>
    <name>A0A8C4LCV3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Myocyte enhancer factor 2B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MEF2B</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LCV3"/>
    <dbReference type="Ensembl" id="ENSEAST00005009996.2">
        <property type="protein sequence ID" value="ENSEASP00005009186.2"/>
        <property type="gene ID" value="ENSEASG00005006578.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160699"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1810.10:FF:000001">
        <property type="entry name" value="Myocyte-specific enhancer factor 2A homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1810.10">
        <property type="entry name" value="Transcription factor, MADS-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002100">
        <property type="entry name" value="TF_MADSbox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036879">
        <property type="entry name" value="TF_MADSbox_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945">
        <property type="entry name" value="MADS BOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11945:SF383">
        <property type="entry name" value="MYOCYTE-SPECIFIC ENHANCER FACTOR 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00319">
        <property type="entry name" value="SRF-TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00404">
        <property type="entry name" value="MADSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00432">
        <property type="entry name" value="MADS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55455">
        <property type="entry name" value="SRF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50066">
        <property type="entry name" value="MADS_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="2" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="MADS-box" evidence="9">
        <location>
            <begin position="32"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="107"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="261"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="285"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="352"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009186.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="382" mass="40178" checksum="7A522FCEA1C0035B" modified="2023-09-13" version="2">MRCARGPFRLLLLPHTTPKPCLLHPARGSWVQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSANRLFQYASTDMDRVLLKYTEYSEPHESRTNTDILETLKRRGVGLDGPELDPDEGPEGPGEKLRRLAGDGGDPALPRPRLYPAAPTMPSPDMVYGALPPPGCEPSGLGEALSAQSRPSPFRPAAPKVGPPGLAHPLFSPSHLASKTPPPLYLAADGRRPDLPGGLAGARGGLSTSRGLYGSLQSPGSAAAPGPPLGSFPFLPAGPPEYGLGDPPPPPGLLQPPTLAPWQPSRGDGPPAGPAQPSGGRSLGEEGPPTRSASPPTPPVSIKSERLSPAPGGPGDFPKAFPYPLLLARPLAEPLRPGPPLRRLSTADSWPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LFY4</accession>
    <name>A0A8C4LFY4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein eL33</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">60S ribosomal protein L35a</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106841435</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Required for the proliferation and viability of hematopoietic cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the large ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eL33 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LFY4"/>
    <dbReference type="Ensembl" id="ENSEAST00005011058.2">
        <property type="protein sequence ID" value="ENSEASP00005010164.1"/>
        <property type="gene ID" value="ENSEASG00005007233.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016972"/>
    <dbReference type="OMA" id="VELFKMY"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.190:FF:000005">
        <property type="entry name" value="60S ribosomal protein L35a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.190">
        <property type="entry name" value="translation elongation factor selb, chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00573">
        <property type="entry name" value="Ribosomal_eL33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001780">
        <property type="entry name" value="Ribosomal_eL33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018266">
        <property type="entry name" value="Ribosomal_eL33_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038661">
        <property type="entry name" value="Ribosomal_eL33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10902">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01247">
        <property type="entry name" value="Ribosomal_L35Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01105">
        <property type="entry name" value="RIBOSOMAL_L35AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005010164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="113" mass="12805" checksum="22FED1713315C836" modified="2022-01-19" version="1">MSRKLWSKAIFTGYKQCLQKQREHTALLKTEGVYAQGETEFYLGKRCAYVYKAKNNKVTPGGKPNKTRVIWGKVTRAHGSSGMVRAKFRSNLPAKAIGHKICVMLDPSRILIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LHI2</accession>
    <name>A0A8C4LHI2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106843838</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the immunoglobulin superfamily. CEA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LHI2"/>
    <dbReference type="Ensembl" id="ENSEAST00005009544.2">
        <property type="protein sequence ID" value="ENSEASP00005008762.1"/>
        <property type="gene ID" value="ENSEASG00005006286.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000263479"/>
    <dbReference type="OMA" id="CHAMNPA"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990782">
        <property type="term" value="F:protein tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002682">
        <property type="term" value="P:regulation of immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20948">
        <property type="entry name" value="IgC2_CEACAM5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05740">
        <property type="entry name" value="IgI_hCEACAM_2_4_6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05774">
        <property type="entry name" value="IgV_CEACAM_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000340">
        <property type="entry name" value="Carcinoembryonic antigen-related cell adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000517">
        <property type="entry name" value="Carcinoembryonic antigen-related cell adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000244">
        <property type="entry name" value="carcinoembryonic antigen-related cell adhesion molecule 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050831">
        <property type="entry name" value="CEA_cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44427:SF1">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44427">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034341874" description="Ig-like domain-containing protein" evidence="7">
        <location>
            <begin position="35"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="141"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="236"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="336" mass="36997" checksum="A9DA7C429D8C51B6" modified="2022-01-19" version="1">MEPPSAPPHRGHIPWQGLLLAVSLISFWNPPTTAQLTIESVPTDAAEGKEVLLVVYNLPGNLLGYGWFKGEIDSNQQIASYVIDTQEMTPGPVNNGQEIIFPNGSLLFQNVTQEDTGYYTLQVIKINFQSELGTGQLHVYPELTKPNITCNSSNPVEHRDPIVLTCEPETQNTTYLWLINNQSLPDSAWLKLSPDNRTLTLLHVTRNDTGPYECETWNPVSARRSDPFYLNVLYGPDAPTISPSDSYYHPGAKLSLSCHAASNPPARYSWLINGRPQQSTQELFIPNISANDSGSYTCLVHNSVTGLNRTTVKTITVSAKGHTQGNGRGLALWPNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LIB4</accession>
    <name>A0A8C4LIB4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Protein phosphatase 1 regulatory subunit 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Protein phosphatase 1 regulatory subunit 22</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PPP1R7</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SDS22 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LIB4"/>
    <dbReference type="Ensembl" id="ENSEAST00005012744.2">
        <property type="protein sequence ID" value="ENSEASP00005011725.2"/>
        <property type="gene ID" value="ENSEASG00005008227.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162473"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000055">
        <property type="entry name" value="Protein phosphatase 1 regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000127">
        <property type="entry name" value="protein phosphatase 1 regulatory subunit 7 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050576">
        <property type="entry name" value="Cilia_flagella_integrity"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973:SF23">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45973">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT SDS22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00365">
        <property type="entry name" value="LRR_SD22"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011725.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="386" mass="44515" checksum="29EF859FD2680FC7" modified="2023-09-13" version="2">MAEERGGGQQQSQEMMEVDRRVESEESGDEEGKKQSSGLVADLSEHSLKDGEEPGDEDPEGEVQWHRWKFGDLCQQLNSGNSECLKEGQELPVDMETINLDKDAEDVDLNHYRIGKIEGFEVLKKVKTLCLRQNLIKCIENLEELQSLRELDLYDNQIKKIENLEALTELEILDISFNLLRNIEGIDKLTRLKKLFLVNNKINKIENISNLRQLQMLELGSNRIRAIENIDALTNLESLFLGKNKITKLQNLDALTNLTVLSMQSNRLTKIEGLQNLVNLRELYLSHNGIEVIEGLENNNKLTMLDVASNRIKKIENISHLTELQEFWMNDNLLESWSDLDELKGAKNLETVYLERNPLQKDPQYRRKIMLALPTVRQIDATFVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LLW2</accession>
    <name>A0A8C4LLW2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA helicase MCM9</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Minichromosome maintenance 9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">MCM9</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Component of the MCM8-MCM9 complex, which forms a hexamer composed of MCM8 and MCM9. Interacts with the DNA mismatch repair (MMR) complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1. Interacts with MLH1; the interaction recruits MLH1 to chromatin. Interacts with MSH2; the interaction recruits MCM9 to chromatin. Interacts with MSH6. Interacts with the MRN complex composed of MRE11, RAD50 and NBN/NBS1; the interaction recruits the MRN complex to DNA damage sites. Interacts with RAD51; the interaction recruits RAD51 to DNA damage sites.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044613043.1">
        <property type="nucleotide sequence ID" value="XM_044757108.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LLW2"/>
    <dbReference type="Ensembl" id="ENSEAST00005014286.2">
        <property type="protein sequence ID" value="ENSEASP00005013147.2"/>
        <property type="gene ID" value="ENSEASG00005009172.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106847011"/>
    <dbReference type="CTD" id="254394"/>
    <dbReference type="GeneTree" id="ENSGT01150000286951"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097362">
        <property type="term" value="C:MCM8-MCM9 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032406">
        <property type="term" value="F:MutLbeta complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032407">
        <property type="term" value="F:MutSalpha complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032408">
        <property type="term" value="F:MutSbeta complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007292">
        <property type="term" value="P:female gamete generation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070716">
        <property type="term" value="P:mismatch repair involved in maintenance of fidelity involved in DNA-dependent DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071168">
        <property type="term" value="P:protein localization to chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036298">
        <property type="term" value="P:recombinational interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd17760">
        <property type="entry name" value="MCM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000671">
        <property type="entry name" value="DNA helicase MCM9 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000120">
        <property type="entry name" value="Probable DNA helicase MCM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058768">
        <property type="entry name" value="MCM9_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF48">
        <property type="entry name" value="DNA HELICASE MCM9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26066">
        <property type="entry name" value="MCM9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11 18" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="20">
        <location>
            <begin position="302"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="656"/>
            <end position="1049"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1061"/>
            <end position="1103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="749"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="804"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="822"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="843"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="863"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="942"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="982"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1032"/>
            <end position="1048"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
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            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="ARBA" id="ARBA00022806"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
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            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00041085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042301"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00065491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005013147.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1144" mass="126560" checksum="14F634E7A96A0350" modified="2023-09-13" version="2">MNIDQVTLVGQVFESYVSEYHKNDILLILKERDEDAHYPVVVNAMTLFETNMEIGEYFNVFPNEVLTIFDSALQRSALTILQSLSQPEGVSMKQNLHARISGLPVCPELVREHIPKTKDVGHFLSVTGTVIRTSPVKVLEFERDYMCNKCKHVFVVKADFEQYYTFCRPSSCPSVEGCDSSKFTCLSGLSSSPTSCRDYQEIKIQEQVQRLSLGSIPRSMKVILEDDLVDSCKSGDDLTIYGVVMQRWKPFQQDVRCEVEIVLKANYVQVNNEQSAGIIMDEEVRKEFEDFWEYHKTDPFAGRNEILASLCPQVFGMYLVKLAVAMVLAGGIQRTDATGTRVRGESHLLLVGDPGTGKSQFLKYAAKITPRSVLTTGIGSTSAGLTVTAVKDSGEWNLEAGALVLADAGLCCIDEFNSLKEHDRTSIHEAMEQQTISVAKAGLVCKLNTRTTILAATNPKGQYDPRESVSVNIALGSPLLSRFDLILVLLDTKNEDWDRIISSFILENKGYPSKSEKLWSMEKMKTYFCLIRNLQPTLSDVGNQVLLRYYQMQRQSDSRNAARTTIRLLESLIRLAEAHARLMFRDTVTLEDAITVVSVMESSMQGGALLGGVNALHTSFPENPLEQYQRQCELILEKLELHSLLSEELRRLERLQSQSACQSQPLAVEGETAPGSSGNDPGEEPEFRTSTQQEIHCGTHAPSAGGSPRGSPLLDPSSHLGPNRSASRKHSAEHKNSRDDSLDWFDSMATHQIEPQNTVPVSPRPKTTGGKMALKISNSKSQGKEKSEPGQGSKLETEQLPAPRETEAPLRPDNGEGKKAKQAAMVSEAAASAEEADSVLTHHVPRKLHRLRQERAQASCRNPARAPSQPASPSHPQPLAVRCPEGRRETPKRRRQKAVAQAEEPELESVESPGPPVAKLAKFTFKRKSRLTHSPEDHSRVSPGTTKIAPQSPKSPHHETRKGAALPVKGPEKLASTSGSRSSDRLQGKTKEVARQPPERDGSGEKRDRAPEKRVTQPELELGNETGCFHPTCERDKKEEVSCSDRSSKVHACTLARLADFSFTSPSESKSKSPPPPERGTCGGRGPSPPPTSTAPLLGRRRKTFQLEGSTARLCLPKNSLFTLPELDDDTLDFDWDEEMRKKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LNC2</accession>
    <name>A0A8C4LNC2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">H/ACA ribonucleoprotein complex subunit DKC1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Dyskerin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Nopp140-associated protein of 57 kDa</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Nucleolar protein NAP57</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Nucleolar protein family A member 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">snoRNP protein DKC1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">DKC1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Required for ribosome biogenesis and telomere maintenance. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>uridine in 5S rRNA = pseudouridine in 5S rRNA</text>
            <dbReference type="Rhea" id="RHEA:47036"/>
            <dbReference type="Rhea" id="RHEA-COMP:11730"/>
            <dbReference type="Rhea" id="RHEA-COMP:11731"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Cajal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pseudouridine synthase TruB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LNC2"/>
    <dbReference type="Ensembl" id="ENSEAST00005011480.2">
        <property type="protein sequence ID" value="ENSEASP00005010549.2"/>
        <property type="gene ID" value="ENSEASG00005007484.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000047092"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031429">
        <property type="term" value="C:box H/ACA snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000495">
        <property type="term" value="P:box H/ACA sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032212">
        <property type="term" value="P:positive regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905323">
        <property type="term" value="P:telomerase holoenzyme complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd02572">
        <property type="entry name" value="PseudoU_synth_hDyskerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21148">
        <property type="entry name" value="PUA_Cbf5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2350.10:FF:000001">
        <property type="entry name" value="H/ACA ribonucleoprotein complex subunit CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012960">
        <property type="entry name" value="Dyskerin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00425">
        <property type="entry name" value="CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003280">
        <property type="entry name" value="PRK04270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08068">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01136">
        <property type="entry name" value="DKCLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dyskerin-like" evidence="14">
        <location>
            <begin position="15"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="PUA" evidence="13">
        <location>
            <begin position="237"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="382"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="406"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00075671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00080839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005010549.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="450" mass="50616" checksum="456A7CD6FDE7FC73" modified="2023-09-13" version="2">MADAEVFILPKKHKKKKERKSLPEEDVATGFINLDKPSNPSSHEVVAWIRRILRVEKTGHSGTLDPKVTGCLIVCIERATRLVKSQQSAGKEYVGIVRLHNAIEGGTQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESKMIEYDPERRLGIFWVSCEAGTYIRTLCVHLGLLLGVGGQMQELRRVRSGVMSEKDHMVTMHDVLDAQWLYDNHKDESYLRRVVYPLEKLLTSHKRLVMKDSAVNAICYGAKIMLPGVLRYEDGIEVNQEIVVITTKGEAICMAIALMTTAVISTCDHGIVAKIKRVIMERDTYPRKWGLGPKASQKRLMIKQGLLDKHGKPTESTPVAWTQEYVDYSDSAKTEVVAEVVKTPKVVAEAVKAPKRKRESESDETSPVSPQLIKKEKKKKKDKKTKAALESAGEPGDRDSDITKKKKKKKKIKVEMLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LQF5</accession>
    <name>A0A8C4LQF5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription elongation factor SPT6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Transcription elongation factor spt6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">SUPT6H</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Histone H3-H4 chaperone that plays a key role in the regulation of transcription elongation and mRNA processing. Enhances the transcription elongation by RNA polymerase II (RNAPII) and is also required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. Besides chaperoning histones in transcription, acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2), to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. SUPT6H via its association with SETD1A, regulates both class-switch recombination and somatic hypermutation through formation of H3K4me3 epigenetic marks on activation-induced cytidine deaminase (AICDA) target loci. Promotes the activation of the myogenic gene program by entailing erasure of the repressive H3K27me3 epigenetic mark through stabilization of the chromatin interaction of the H3K27 demethylase KDM6A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the SPT6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014717566.1">
        <property type="nucleotide sequence ID" value="XM_014862080.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014717568.1">
        <property type="nucleotide sequence ID" value="XM_014862082.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014717569.1">
        <property type="nucleotide sequence ID" value="XM_014862083.3"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005015428.2">
        <property type="protein sequence ID" value="ENSEASP00005014190.1"/>
        <property type="gene ID" value="ENSEASG00005009905.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106844574"/>
    <dbReference type="KEGG" id="eai:106844574"/>
    <dbReference type="CTD" id="6830"/>
    <dbReference type="GeneTree" id="ENSGT00510000047446"/>
    <dbReference type="OrthoDB" id="343921at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001825">
        <property type="term" value="P:blastocyst formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034728">
        <property type="term" value="P:nucleosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045191">
        <property type="term" value="P:regulation of isotype switching"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051147">
        <property type="term" value="P:regulation of muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140673">
        <property type="term" value="P:transcription elongation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00164">
        <property type="entry name" value="S1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09928">
        <property type="entry name" value="SH2_Cterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09918">
        <property type="entry name" value="SH2_Nterm_SPT6_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000494">
        <property type="entry name" value="Suppressor of Ty 6 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2740:FF:000001">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.650:FF:000002">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.850:FF:000002">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3500.10:FF:000001">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.140:FF:000004">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000030">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000032">
        <property type="entry name" value="Transcription elongation factor spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.650">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2740">
        <property type="entry name" value="Spt6, Death-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.850">
        <property type="entry name" value="Spt6, helix-hairpin-helix domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3500.10">
        <property type="entry name" value="Tex N-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.140">
        <property type="entry name" value="YqgF/RNase H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041692">
        <property type="entry name" value="HHH_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028083">
        <property type="entry name" value="Spt6_acidic_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042066">
        <property type="entry name" value="Spt6_death-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032706">
        <property type="entry name" value="Spt6_HHH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028088">
        <property type="entry name" value="Spt6_HTH_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035420">
        <property type="entry name" value="Spt6_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035018">
        <property type="entry name" value="Spt6_SH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035019">
        <property type="entry name" value="Spt6_SH2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028231">
        <property type="entry name" value="Spt6_YqgF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055179">
        <property type="entry name" value="Tex-like_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023323">
        <property type="entry name" value="Tex-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023319">
        <property type="entry name" value="Tex-like_HTH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017072">
        <property type="entry name" value="TF_Spt6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006641">
        <property type="entry name" value="YqgF/RNaseH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037027">
        <property type="entry name" value="YqgF/RNaseH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10145:SF6">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14635">
        <property type="entry name" value="HHH_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17674">
        <property type="entry name" value="HHH_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14641">
        <property type="entry name" value="HTH_44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14633">
        <property type="entry name" value="SH2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14632">
        <property type="entry name" value="SPT6_acidic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22706">
        <property type="entry name" value="Tex_central_region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14639">
        <property type="entry name" value="YqgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036947">
        <property type="entry name" value="Spt6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00732">
        <property type="entry name" value="YqgFc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158832">
        <property type="entry name" value="Tex N-terminal region-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="11" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="9 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="S1 motif" evidence="18">
        <location>
            <begin position="1227"/>
            <end position="1282"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="17">
        <location>
            <begin position="1325"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="484"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1636"/>
            <end position="1726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="126"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="149"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="495"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="510"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="1639"/>
            <end position="1664"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009253"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020248"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
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            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
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            <dbReference type="ARBA" id="ARBA00022664"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
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            <dbReference type="ARBA" id="ARBA00022816"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
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            <dbReference type="ARBA" id="ARBA00023163"/>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
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            <dbReference type="ARBA" id="ARBA00023242"/>
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    <evidence type="ECO:0000256" key="13">
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            <dbReference type="ARBA" id="ARBA00070625"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRNR" id="PIRNR036947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1726" mass="199131" checksum="0BF722804CCD94C8" modified="2022-01-19" version="1">MSDFVESEAEESEEEYNDEGEVVSRVTKKFVEEEDDDEEEEEENLDDQDEQGNLKGFINDDDDEDEGEEDEGSDSGDSEDDVGHKKRKRTSFDDRLEDDDFDLIEENLGVKVKRGQKYRRVKKMSDDEDDDEEEYGKEEHEKEAIAEEIFQDGEGEEGQEAVEAPMAPPEEEEEDDEESDIDDFIVDDDGQPLKKPKWRKKLPGYTDAALQEAQEIFGVDFDYDEFEKYNEYDEELEEEYEYEDDEAEGEIRVRPKKTTKKRVSRRSIFEMYEPSELESSHLTDQDNEIRATDLPERFQLRSIPVKGAEDDELEEEADWIYRNAFATPTISLQESCDYLDRGQPTSTFSRKGPSTIQKIKEALGFMRNQHFEVPFIAFYRKEYVEPELHINDLWRVWQWDEKWTQLRIRKENLTRLFEKMQAYQYEQISADPDKPLADGIRALDTTDMERLKDVQSMDELKDVYNHFLLYYGRDIPKMQNAAKASRKKLKRVREEGDEEGEGEEAEDEEQRGPELKQASRRDMYTICQSAGLDGLAKKFGLTPEQFGENLRDSYQRHETEQFPAEPLELAKDYVCSQFPTPEAVLEGARYMVALQIAREPLVRQVLRQTFQERAKLNITPTKKGRKDVDEAHYAYSFKYLKNKPVKELRDDQFLKICLAEDEGLLIIDISIDMKGVEGYGNDQTYFEEIKQFYYRDEFSHQVQEWNRQRTMAIERALQQFLYVQMAKELKNKLLAEAKEYVIKACSRKLYNWLRVAPYRPDQQVEEDDDFMDENQGKGIRVLGIAFSSARDHPVFCALVNGEGEVTDFLRLPHFTKRRTAWREEEREKKAQDIETLKKFLLNKKPHVVTVAGENRDAQMLIEDVKRIVHELDQGQQLSSIGVELVDNELAILYMNSKKSEAEFRDYPPVLRQAVSLARRIQDPLIEFAQVCSSDEDILCLKFHPLQEHVVKEELLNALYCEFINRVNEVGVDVNRAIAHPYSQALIQYVCGLGPRKGTHLLKILKQNNTRLESRTQLVTMCHMGPKVFMNCAGFLKIDTASLGDSTDSYIEVLDGSRVHPETYEWARKMAVDALEYDESAEDANPAGALEEILENPERLKDLDLDAFAEELERQGYGDKHITLYDIRAELSCRYKDLRTAYRSPNTEEIFNMLTKETPETFYIGKLIICNVTGIAHRRPQGESYDQAIRNDETGLWQCPFCQQDNFPELSEVWNHFDSGSCPGQAIGVKTRLDNGVTGFIPTKFLSDKVVKRPEERVKVGMTVHCRIMKIDIEKFSADLTCRTSDLMDRNNEWKLPKDTYYDFDAEAADHKQEEDMKRKQQRTTYIKRVIAHPSFHNINFKQAEKMMETMDQGDVIIRPSSKGENHLTVTWKVSDGIYQHVDVREEGKENAFSLGATLWINSEEFEDLDEIVARYVQPMASFARDLLNHKYYQDCSGGDRKKLEELLIKTKKEKPTFIPYFICACKELPGKFLLGYQPRGKPRIEYVTVTPEGFRYRGQIFPTVNGLFRWFKDHYQDPVPGITPSSSSRTRTPASINATPANINLADLTRAVNALPQNMTSQMFSAIAAVTGQGQNPNATPAQWASSQYGYGGSGGGSSAYHVFPTPAQQPVATPLMTPSYSYTTPSQPITTPQYHQLQASTTPQSAQAQPQPSSSSRQRQQQPKSNSHAAIDWGKMAEQWLQEKEAERRKQKQRLTPRPSPSPMIESTPMSIAGDATPLLDEMDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LVB4</accession>
    <name>A0A8C4LVB4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">E3 ubiquitin-protein ligase DZIP3</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">RING-type E3 ubiquitin transferase DZIP3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">DZIP3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Able to specifically bind RNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 22">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4LVB4"/>
    <dbReference type="Ensembl" id="ENSEAST00005017738.2">
        <property type="protein sequence ID" value="ENSEASP00005016331.2"/>
        <property type="gene ID" value="ENSEASG00005011304.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161692"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019902">
        <property type="term" value="F:phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd16460">
        <property type="entry name" value="RING-H2_DZIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000308">
        <property type="entry name" value="E3 ubiquitin-protein ligase DZIP3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033103">
        <property type="entry name" value="DZIP3_RING-H2_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041249">
        <property type="entry name" value="HEPN_DZIP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056872">
        <property type="entry name" value="TTC3/DZIP3-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056870">
        <property type="entry name" value="TTC3/DZIP3/RBM44-like_helical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043866">
        <property type="entry name" value="TTC3/DZIP3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15727:SF4">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE DZIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15727">
        <property type="entry name" value="RING FINGER PROTEIN 214"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18738">
        <property type="entry name" value="HEPN_DZIP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24525">
        <property type="entry name" value="TTC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24905">
        <property type="entry name" value="TTC3_9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19179">
        <property type="entry name" value="TTC3_DZIP3_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="1147"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="634"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1087"/>
            <end position="1138"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="805"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="8"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="647"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="657"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="669"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1088"/>
            <end position="1113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071921"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00078162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005016331.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1207" mass="138375" checksum="DAD36B3FC2B9BFFE" modified="2023-09-13" version="2">MDSLPEEFFVRDHPVEEQTEETENKVEKSTDQLDKQENDTPTDLVPVNLLLEVKKLLNAINTLPKGVVPHIKKFLQEDFSFQTIQREIAANSQNGEEVVPALTLRFLITQLEAALRNIQAPNYTAHQINIGYYLTLLFLYGVALTERGKKEDYTEAENKFLVMKMVIQENEICENFMSLVYFGRGLLRCAQKRYNGGLLEFHKSLQEIGDTNDHWFDIDPTEDEDLPTTFKDLLNNFIKTTESNIMKLTICSYLDCERSCEADILKNTNYKGFFQLTCSKSCCVYFHKICWKKFKNLKYPGENDQSFSGKKCLKEGCTGDMVRMLQCDVPGIVKILFEVVRKDEYITIENLGASYKKLMSLKITDTDIRPKISLKFNTKDEMPIFKLDYNYFYHLLHIIIISGTDIVRQIFDEALPPPLLKKELLIHKNVLEPYYNHLWTNHPLGGAWHLLYPPKKELPQSKQFDLYLLLALIKHLNVFPAPKKGWNMEPPSSDLSKSADILRLCKYRDILLSEILMNGLTESQFNSIWKKVSDILLRLGMKQEDIDKVKENPIENISLDYHQLSIYLGIPVPEIIQRMLSCYQQGIALQSITGSQRIEIEELQNEEEELSPPLMEYNINVKSNPEIQLTEMNKDGASIPSESSTESVKDLQEVKSKPKKKKKTKNKKNKESKEEQVPKMVGKEEQLKKEPVNLHPVSRFMKDDASDVQEDSATEDKFYSLDELHILDMIEQGSTSKVTAEYGETEKEKLARQRQLYKLHYQCEDFKRQLKTVTFRWQENQMQIKKKDKIIASLNQQVAFGINKVSKLQRQSHAKDNEIKNLKEQLSMKRSQWEMEKHNLESTIKTYLNKLNAETSRALTAEVYFLQCRRDFGLLHLEQTEKECLSQLARVTHMAASNLESLQLKAAVDSWNAIVTDVRNKIAFLRTQYNEQINKVKQGFALSTLPPIQLPPPPPSPEILMQQFLGRPLVKESFFRPILTVPQMPAVCPGVISAPGQPRAPLMTGIAWTVPAPVGEAVPPSAGLGSDAPVMNWERITDRLKTAFPQQTRKELTDFLRKLKDAHGKSLSGMTFDEIVYKISQFIDPKRSQNQGKSVPNGNCVSPSHTPLQSNAAQPPKPAWRPLNSQGPATWEGANNLDGEEEEEEPCVICHENLSPENLSVLPCAHKFHSQCIRPWLMQQGTCPTCRLHVLLPEEFPGHPNRHLPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LVQ9</accession>
    <name>A0A8C4LVQ9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Granulocyte colony-stimulating factor receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CSF3R</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">Homodimer. The dimeric receptor binds two CSF3 molecules. Interacts with CEACAM1; down-regulates the CSF3R-STAT3 pathway through recruitment of PTPN6 that dephosphorylates CSF3R.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LVQ9"/>
    <dbReference type="Ensembl" id="ENSEAST00005019167.2">
        <property type="protein sequence ID" value="ENSEASP00005017649.1"/>
        <property type="gene ID" value="ENSEASG00005012209.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158915"/>
    <dbReference type="OMA" id="SYCSIPR"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030666">
        <property type="term" value="C:endocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051916">
        <property type="term" value="F:granulocyte colony-stimulating factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030593">
        <property type="term" value="P:neutrophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045637">
        <property type="term" value="P:regulation of myeloid cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000879">
        <property type="entry name" value="Colony stimulating factor 3 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001209">
        <property type="entry name" value="Colony stimulating factor 3 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001234">
        <property type="entry name" value="Colony stimulating factor 3 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000997">
        <property type="entry name" value="Colony stimulating factor 3 receptor (Granulocyte)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000465">
        <property type="entry name" value="Granulocyte colony-stimulating factor receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000839">
        <property type="entry name" value="granulocyte colony-stimulating factor receptor isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003529">
        <property type="entry name" value="Hematopoietin_rcpt_Gp130_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010457">
        <property type="entry name" value="IgC2-like_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052672">
        <property type="entry name" value="Type1_Cytokine_Rcpt_Type2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48423:SF1">
        <property type="entry name" value="INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06328">
        <property type="entry name" value="Lep_receptor_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01353">
        <property type="entry name" value="HEMATOPO_REC_L_F2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 16" id="KW-0732">Signal</keyword>
    <keyword evidence="3 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034069715" description="Granulocyte colony-stimulating factor receptor" evidence="16">
        <location>
            <begin position="25"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="631"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="234"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="334"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="433"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="529"/>
            <end position="626"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005017649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="841" mass="92588" checksum="E629E4E3652B795E" modified="2022-01-19" version="1">MAGLGTRNLIRAALVILLLPGSLEECGRIKVSAPIVRLGDPITASCIINQSCSHLGPEAQILWKLGAELQPGGRQQRLADGTQESTITLPHLNHSQALLSCCLRWGNSLQILDQAELQAGYPPATPHNLSCAMNLTTSSLICQWEPGPDTHLPTNFTLKSFKSRRGCQTQEDPITNCVPENGQSRCSIPRKHLQLYQNMSIWVQVENALGTSVSPQLCLVPMDVVKLEPPTLWALDPKTEVVPLQPGCLRLRWEPWKPGLYIEQKCELRHQPQLGETSWALVGPLPSRTLQHELCGLLPSTAYALQMRCTRWPLPGLWSNWSPSLELTTAQRAPIVRLDTWWRQRQLDPRTVDVQLFWKPTPLEEDSGQIQGYLVSWRPSGQAEAVPPLCNTTELNCTFHLPSEARKVVLKAYNTAGTSRPTPVVFLESRGPPLGRLHTMARDPHSLWVGWEPPNPQPRGYVIEWGLGPPSPSGSHQTWRMEHNGSIAGTLLQENIRPFQLYAITVTPLYQDTKGPSQLIYAYSQETAPSRAPELHLKHIGKTWAKLEWVPEAPELGKSPLTHYTIFWTNAQDQSFSTVLNASSHDFVLHGLEPASLYHVHLMAASRAGATNSTSLTLMTLVLEESELHTLLGLFGLLLLFICLCGAVRLCCRPSRKNALWPSVPDPAHSSLGSWVPTIMAEETFQLPSLCDASMPPITKITVLEEEEKKPGPWESSDSSGTCGLPTLVQAYVLQGDPRAASTPPEPQSGTSNQVLYGQVLGSPTSPGPGHYLRCDSTQPLLGGLTPSPKCYENLWFQTSPLGTSEPLVPNQEDDCVFGPLLDFPLLQGLRINGVEGLGGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LXD1</accession>
    <name>A0A8C4LXD1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Lysosomal Pro-X carboxypeptidase</fullName>
            <ecNumber evidence="14">3.4.16.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Proline carboxypeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Prolylcarboxypeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">PRCP</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Cleaves C-terminal amino acids linked to proline in peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Cleavage of a -Pro-|-Xaa bond to release a C-terminal amino acid.</text>
            <dbReference type="EC" id="3.4.16.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S28 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.2" evidence="14"/>
    <dbReference type="RefSeq" id="XP_014695697.1">
        <property type="nucleotide sequence ID" value="XM_014840211.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014695707.1">
        <property type="nucleotide sequence ID" value="XM_014840221.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070346666.1">
        <property type="nucleotide sequence ID" value="XM_070490565.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LXD1"/>
    <dbReference type="Ensembl" id="ENSEAST00005020078.2">
        <property type="protein sequence ID" value="ENSEASP00005018505.1"/>
        <property type="gene ID" value="ENSEASG00005012757.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106830586"/>
    <dbReference type="KEGG" id="eai:106830586"/>
    <dbReference type="CTD" id="5547"/>
    <dbReference type="GeneTree" id="ENSGT00940000158099"/>
    <dbReference type="OMA" id="QTCNQMV"/>
    <dbReference type="OrthoDB" id="2130629at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045178">
        <property type="term" value="C:basal part of cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008239">
        <property type="term" value="F:dipeptidyl-peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004181">
        <property type="term" value="F:metallocarboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060055">
        <property type="term" value="P:angiogenesis involved in wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002003">
        <property type="term" value="P:angiotensin maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097009">
        <property type="term" value="P:energy homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042593">
        <property type="term" value="P:glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003085">
        <property type="term" value="P:negative regulation of systemic arterial blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002353">
        <property type="term" value="P:plasma kallikrein-kinin cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043535">
        <property type="term" value="P:regulation of blood vessel endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000377">
        <property type="term" value="P:regulation of reactive oxygen species metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002155">
        <property type="term" value="P:regulation of thyroid hormone receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.980:FF:000002">
        <property type="entry name" value="lysosomal Pro-X carboxypeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.980">
        <property type="entry name" value="Serine carboxypeptidase S28, SKS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008758">
        <property type="entry name" value="Peptidase_S28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042269">
        <property type="entry name" value="Ser_carbopepase_S28_SKS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010:SF38">
        <property type="entry name" value="LYSOSOMAL PRO-X CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11010">
        <property type="entry name" value="PROTEASE S28 PRO-X CARBOXYPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05577">
        <property type="entry name" value="Peptidase_S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0458">Lysosome</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034976828" description="Lysosomal Pro-X carboxypeptidase" evidence="18">
        <location>
            <begin position="20"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00052013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00076475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00076608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005018505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="497" mass="56350" checksum="28F2BA28477AD3D4" modified="2022-01-19" version="1">MERRALLLLLLAFLTPGAPIPVPPALKALSNLHLSTRLTSRPAIDIKYSIHYIQQKVDHFGFDTDKTFKQRYLIADKHWKKDGGSILFYTGNEGDITWFCNNTGFMWDMAEELKAMLVFAEHRYYGESLPFGANSFKDSTHLNFLTSEQALADFAKLIKHLKRTVPGAKNQPVIALGGSYGGMLAAWFRMKYPHMVVGALASSAPIWQFEDLVPCGVFMEIVTADFRKSGPNCSESIRRSWDAIRRLTSTGPGLHWLSEALHLCSPLTNSQDVEHLKDWITETWVNLAMVDYPYESNFLQPLPAWPIKVVCQYLKNPNVSDPQLLQNIFQALNVYYNYSGQVKCLNMSETATRSLGSLGWSYQACTEMVMPFCTNGIDDMFEPHSWNLKEFSDDCFKQWGVRPRPSWITTMYGGKNISSHTNIIFSNGDLDPWSGGGVTENVTDTLVAITIPEGAHHLDLRANNAFDPTSVLLARSLEVRYMKQWIKNFYASLRKKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M8P3</accession>
    <name>A0A8C4M8P3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 47">Phospholipase A2</fullName>
            <ecNumber evidence="6 47">3.1.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="50" type="primary">PLA2G4D</name>
    </gene>
    <organism evidence="50 51">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="50 51" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="50" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="50" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>1,2-di-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + H2O = 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41075"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:60657"/>
            <dbReference type="ChEBI" id="CHEBI:74344"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="40">
            <dbReference type="Rhea" id="RHEA:41076"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="42">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + H2O = 1-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41123"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:72828"/>
            <dbReference type="ChEBI" id="CHEBI:75163"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="42">
            <dbReference type="Rhea" id="RHEA:41124"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="44">
            <text>1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-2-O-hexadecyl-sn-glycero-3-phosphocholine + H2O = 2-O-hexadecyl-sn-glycero-3-phosphocholine + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41271"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:77695"/>
            <dbReference type="ChEBI" id="CHEBI:77696"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="44">
            <dbReference type="Rhea" id="RHEA:41272"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-2-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41071"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:74344"/>
            <dbReference type="ChEBI" id="CHEBI:77694"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:41072"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>1-O-hexadecyl-2-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphocholine + H2O = 1-O-hexadecyl-sn-glycero-3-phosphocholine + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41067"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:55430"/>
            <dbReference type="ChEBI" id="CHEBI:64496"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="41">
            <dbReference type="Rhea" id="RHEA:41068"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40427"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73003"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="36">
            <dbReference type="Rhea" id="RHEA:40428"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = (9Z,12Z)-octadecadienoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40811"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73002"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:40812"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + H2O = 1-octadecanoyl-sn-glycero-3-phosphate + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40451"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:74565"/>
            <dbReference type="ChEBI" id="CHEBI:77091"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:40452"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="43">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + H2O = 1-octadecanoyl-sn-glycero-3-phosphocholine + (5Z,8Z,11Z,14Z)-eicosatetraenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40519"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:73858"/>
            <dbReference type="ChEBI" id="CHEBI:74965"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="43">
            <dbReference type="Rhea" id="RHEA:40520"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine + glycerol = 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + 1-octadecanoyl-sn-glycero-3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:41099"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:73858"/>
            <dbReference type="ChEBI" id="CHEBI:74965"/>
            <dbReference type="ChEBI" id="CHEBI:75612"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:41100"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>1-octadecanoyl-2-(9Z,12Z,15Z-octadecatrienoyl)-sn-glycero-3-phosphocholine + H2O = (9Z,12Z,15Z)-octadecatrienoate + 1-octadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41307"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32387"/>
            <dbReference type="ChEBI" id="CHEBI:73858"/>
            <dbReference type="ChEBI" id="CHEBI:78022"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:41308"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>1-octadecanoyl-2-(9Z,12Z,15Z-octadecatrienoyl)-sn-glycero-3-phosphocholine + glycerol = 1-(9Z,12Z,15Z-octadecatrienoyl)-glycerol + 1-octadecanoyl-sn-glycero-3-phosphocholine</text>
            <dbReference type="Rhea" id="RHEA:41087"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:73858"/>
            <dbReference type="ChEBI" id="CHEBI:75610"/>
            <dbReference type="ChEBI" id="CHEBI:78022"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:41088"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="45">
            <text>2-(prostaglandin E2)-sn-glycero-3-phosphocholine + H2O = prostaglandin E2 + sn-glycerol 3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53692"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:137585"/>
            <dbReference type="ChEBI" id="CHEBI:606564"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="45">
            <dbReference type="Rhea" id="RHEA:53693"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>2-(prostaglandin E2)-sn-glycero-3-phosphoethanolamine + H2O = sn-glycero-3-phosphoethanolamine + prostaglandin E2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:137581"/>
            <dbReference type="ChEBI" id="CHEBI:143890"/>
            <dbReference type="ChEBI" id="CHEBI:606564"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:53705"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>2-[(11R)-hydroxy-(5Z,8Z,12E,14Z)-eicosatetraenoyl]-sn-glycero-3-phosphocholine + H2O = (11R)-hydroxy-(5Z,8Z,12E,14Z)-eicosatetraenoate + sn-glycerol 3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53688"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:78836"/>
            <dbReference type="ChEBI" id="CHEBI:137582"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:53689"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>2-[(15R)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl]-sn-glycero-3-phosphocholine + H2O = (15R)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + sn-glycerol 3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53696"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:78837"/>
            <dbReference type="ChEBI" id="CHEBI:137583"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:53697"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>2-[(15S)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoyl]-sn-glycero-3-phosphocholine + H2O = (15S)-hydroxy-(5Z,8Z,11Z,13E)-eicosatetraenoate + sn-glycerol 3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:57409"/>
            <dbReference type="ChEBI" id="CHEBI:137584"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:53701"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:15802"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>a 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine + H2O = a 1-O-alkyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36231"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:30909"/>
            <dbReference type="ChEBI" id="CHEBI:36702"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:36232"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="38">
            <dbReference type="Rhea" id="RHEA:15178"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="28">Lipid metabolism; arachidonate metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Lipid metabolism; leukotriene B4 biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism; prostaglandin biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="29">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="24">Phospholipid metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="30">Interacts with KAT5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="47">The N-terminal C2 domain associates with lipid membranes upon calcium binding.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.4" evidence="6 47"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4M8P3"/>
    <dbReference type="Ensembl" id="ENSEAST00005024051.2">
        <property type="protein sequence ID" value="ENSEASP00005022166.2"/>
        <property type="gene ID" value="ENSEASG00005015140.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106841343"/>
    <dbReference type="KEGG" id="eai:106841343"/>
    <dbReference type="CTD" id="283748"/>
    <dbReference type="GeneTree" id="ENSGT01030000234606"/>
    <dbReference type="OrthoDB" id="270970at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047498">
        <property type="term" value="F:calcium-dependent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019370">
        <property type="term" value="P:leukotriene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001516">
        <property type="term" value="P:prostaglandin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04036">
        <property type="entry name" value="C2_cPLA2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Phospholipase A2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Phospholipase A2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041847">
        <property type="entry name" value="C2_cPLA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040723">
        <property type="entry name" value="cPLA2_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF31">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2 DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18695">
        <property type="entry name" value="cPLA2_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16 47" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 47" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="20" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="20" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="14" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="15 46 47" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0434">Leukotriene biosynthesis</keyword>
    <keyword evidence="9" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="17 46 47" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="18 46 47" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
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    <keyword evidence="11" id="KW-0595">Phospholipid degradation</keyword>
    <keyword evidence="21" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="10" id="KW-0643">Prostaglandin biosynthesis</keyword>
    <keyword evidence="8" id="KW-0644">Prostaglandin metabolism</keyword>
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            <begin position="5"/>
            <end position="124"/>
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            <begin position="273"/>
            <end position="817"/>
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            <dbReference type="ARBA" id="ARBA00022668"/>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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            <dbReference type="ARBA" id="ARBA00022751"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8C4M9P3_EQUAS</name>
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        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
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        <name evidence="7" type="primary">LOC106824167</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
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    <dbReference type="InterPro" id="IPR050441">
        <property type="entry name" value="RBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012604">
        <property type="entry name" value="RBM1CTR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48034">
        <property type="entry name" value="TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08081">
        <property type="entry name" value="RBM1CTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
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            <end position="86"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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        <source>
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    <evidence type="ECO:0000256" key="4">
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    <evidence type="ECO:0000256" key="5">
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020749.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
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    <sequence length="392" mass="43282" checksum="F3B6A0A421F8E19D" modified="2022-01-19" version="1">MMEADQPGKLFIGGLNTQTTEKALAAIFGKYGHVMEVLLIKDRETNKSRGFAFITFESPADAKNAAKEMNGKSLDGKAIKVEQANKPSFESGGRRRPRLHSKNRGHPRSLKCRRAESGGERRRPSRGGHLDEGGYSLNLNMSSTRGRFPVKRGPSSRSRSPPPQRSAPSAPGRSSSGMEGRGPVSRGRENYGGPPRREPVSSRRGDYVSPRDDGYTAKDSYSSRDYPRSRDTKDYTPPPRDSAYRDYGRSSSREEHSSRGYSGRDSYGGGRDRDYSEHPGGGSYRDSYESYGSSRGAPPPRAPPLTYDGSSRYDEYSNTRDGYGGGRESYSSSRNDSYLSAREHGGRQERGFPHYVYRVYPAPRESYSTSSCGASRGGSGGSRSEREGRSRY</sequence>
</entry>
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            <fullName evidence="13">General transcription factor II-I repeat domain-containing protein 1</fullName>
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            <taxon>Metazoa</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="11">May be a transcription regulator involved in cell-cycle progression and skeletal muscle differentiation. May repress GTF2I transcriptional functions, by preventing its nuclear residency, or by inhibiting its transcriptional activation. May contribute to slow-twitch fiber type specificity during myogenesis and in regenerating muscles. Binds troponin I slow-muscle fiber enhancer (USE B1). Binds specifically and with high affinity to the EFG sequences derived from the early enhancer of HOXC8.</text>
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    <comment type="subunit">
        <text evidence="12">Interacts with the retinoblastoma protein (RB1) via its C-terminus.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="ENSEASG00005015852.2"/>
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        <property type="component" value="Chromosome 14"/>
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    <dbReference type="GO" id="GO:0000978">
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        <property type="entry name" value="TF_II-I"/>
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        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR II-I REPEAT DOMAIN-CONTAINING PROTEIN 1"/>
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    </dbReference>
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        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR II-I REPEAT DOMAIN-CONTAINING PROTEIN 1"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MGL7</accession>
    <name>A0A8C4MGL7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Forkhead box protein M1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">FOXM1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Transcription factor regulating the expression of cell cycle genes essential for DNA replication and mitosis. Plays a role in the control of cell proliferation. Also plays a role in DNA break repair, participating in the DNA damage checkpoint response. Promotes transcription of PHB2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044611803.1">
        <property type="nucleotide sequence ID" value="XM_044755868.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MGL7"/>
    <dbReference type="Ensembl" id="ENSEAST00005027323.2">
        <property type="protein sequence ID" value="ENSEASP00005025168.2"/>
        <property type="gene ID" value="ENSEASG00005017130.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831511"/>
    <dbReference type="CTD" id="2305"/>
    <dbReference type="GeneTree" id="ENSGT00940000158804"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030330">
        <property type="term" value="P:DNA damage response, signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032873">
        <property type="term" value="P:negative regulation of stress-activated MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000781">
        <property type="term" value="P:positive regulation of double-strand break repair"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007346">
        <property type="term" value="P:regulation of mitotic cell cycle"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046578">
        <property type="term" value="P:regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000377">
        <property type="term" value="P:regulation of reactive oxygen species metabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd20029">
        <property type="entry name" value="FH_FOXM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000245">
        <property type="entry name" value="forkhead box protein M1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR047516">
        <property type="entry name" value="FH_FOXM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042839">
        <property type="entry name" value="FOXM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018122">
        <property type="entry name" value="TF_fork_head_CS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030456">
        <property type="entry name" value="TF_fork_head_CS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46878">
        <property type="entry name" value="FORKHEAD BOX PROTEIN M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46878:SF1">
        <property type="entry name" value="FORKHEAD BOX PROTEIN M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00657">
        <property type="entry name" value="FORK_HEAD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00658">
        <property type="entry name" value="FORK_HEAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="14">
        <location>
            <begin position="242"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="12">
        <location>
            <begin position="242"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
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            <begin position="109"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="13">
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            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="471"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="543"/>
            <end position="633"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="169"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
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            <end position="512"/>
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        <location>
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            <end position="592"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
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            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025168.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MGW0</accession>
    <name>A0A8C4MGW0_EQUAS</name>
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        <recommendedName>
            <fullName evidence="9">Trans-2,3-enoyl-CoA reductase-like</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Steroid 5-alpha-reductase 2-like 2 protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TECRL</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MGW0"/>
    <dbReference type="Ensembl" id="ENSEAST00005028889.2">
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        <property type="gene ID" value="ENSEASG00005018158.2"/>
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    <dbReference type="GeneTree" id="ENSGT00950000182886"/>
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        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016627">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17125">
        <property type="entry name" value="Ubl_TECRL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000131">
        <property type="entry name" value="trans-2,3-enoyl-CoA reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001104">
        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039357">
        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049127">
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        <property type="entry name" value="TECRL_Ubl"/>
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        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
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    <dbReference type="PANTHER" id="PTHR10556:SF27">
        <property type="entry name" value="TRANS-2,3-ENOYL-COA REDUCTASE-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
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        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="TECR-like N-terminal" evidence="14">
        <location>
            <begin position="60"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="3-oxo-5-alpha-steroid 4-dehydrogenase C-terminal" evidence="13">
        <location>
            <begin position="212"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00070012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF02544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF21696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026609.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="354" mass="41105" checksum="20FDE17C8711AD42" modified="2023-09-13" version="2">MFKRHKSLASERKKELSSHGATRSTLKNDMRNFHFLSQLILSAGPLKSIPAIKHSKTAHFEVEILDAHTRKQICIVDKVTQTSTIHDVKQKFHKACPKWYPSRIGLQLERGGPFLKDYVTIQSVAASSIVTLYFTDLGQQVSWTTVFLAEYTGPLLIYLLFYLRIPYIYDMKECSRRLRHPVVHLACFCHCMHYIRYLLETLFVHKVSAGHTPLKNLIKGCVFYWGFTSWMAYYINHPRYTPPSFGNRQITVSAINFLICEAGNHFINVVLAHPNHTGNNACFPSPNYNPFTWMFFLVSCPNYTYELTIMLPVGIFTLLMTIQMSLWAQKKHKVYLKKFNSYMRRKSAMIPFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MHI6</accession>
    <name>A0A8C4MHI6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106846888</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cell membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MHI6"/>
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        <property type="protein sequence ID" value="ENSEASP00005026875.2"/>
        <property type="gene ID" value="ENSEASG00005018317.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162704"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15918">
        <property type="entry name" value="7tmA_OR1_7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000009">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48001">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="197"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026875.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="313" mass="34327" checksum="641F704E72214D0E" modified="2023-09-13" version="2">MEGANLTWVSEFLLLGLSEDPKQQQLLFILFLSVYLVTGLGNLLIVLVITSDIQLHTPMYFFLANLAFVDICFTSTTIPKMLANHVSGHKGIPSAGCLTQIFFFIWFAGIDSFLLTAMAYDRYAAICHPLHYATSVTPQLCGHLVAASWTAAFGNALTHTVLLTRLSFCSHNRVPHFFCDLSPLLKLACSDIFLNNAMVYTVGALSVIAPFVGILVSYTHIFAAVLRIPSTRGKRKAFSTCGSHLCVVSLFYGTLIGVYFNPTSSHTAQKDTAAAVMYSVVTPMLNPFIYSLRNNDMKSALGALISKRSVFIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MJ41</accession>
    <name>A0A8C4MJ41_EQUAS</name>
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        <recommendedName>
            <fullName evidence="11">P2Y purinoceptor 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">P2RY6</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044608220.1">
        <property type="nucleotide sequence ID" value="XM_044752285.2"/>
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        <property type="nucleotide sequence ID" value="XM_070490745.1"/>
    </dbReference>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_070490747.1"/>
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        <property type="nucleotide sequence ID" value="XM_070490750.1"/>
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    <dbReference type="GeneTree" id="ENSGT01030000234621"/>
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        <property type="component" value="Chromosome 20"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:cellular response to pyrimidine ribonucleotide"/>
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    <dbReference type="CDD" id="cd15379">
        <property type="entry name" value="7tmA_P2Y6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000209">
        <property type="entry name" value="p2Y purinoceptor 6"/>
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    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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    <dbReference type="InterPro" id="IPR001973">
        <property type="entry name" value="P2Y6_rcpt"/>
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        <property type="entry name" value="P2Y PURINOCEPTOR 6"/>
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    </dbReference>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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        <property type="entry name" value="P2Y6PRNOCPTR"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
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    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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            <end position="52"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="43"/>
            <end position="301"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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            <dbReference type="ARBA" id="ARBA00023040"/>
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            <dbReference type="SAM" id="Phobius"/>
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</entry>
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        <recommendedName>
            <fullName evidence="9">Bridging integrator 3</fullName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in cytokinesis and septation where it has a role in the localization of F-actin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MKZ3"/>
    <dbReference type="Ensembl" id="ENSEAST00005029980.2">
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        <property type="gene ID" value="ENSEASG00005018792.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182882"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
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    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048589">
        <property type="term" value="P:developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097320">
        <property type="term" value="P:plasma membrane tubulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000028">
        <property type="entry name" value="Bridging integrator 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046982">
        <property type="entry name" value="BIN3/RVS161-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47174:SF3">
        <property type="entry name" value="BRIDGING INTEGRATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03114">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00721">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51021">
        <property type="entry name" value="BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0717">Septation</keyword>
    <feature type="domain" description="BAR" evidence="12">
        <location>
            <begin position="1"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="179"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005027604.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="205" mass="23950" checksum="568D1FF59ACB060F" modified="2023-09-13" version="2">MENFSTMSKSAVKISLDLLSNPLCEQDQDFLNMVTALDTAMKRMDAFNQEKVNQIQKTVIEPLKKFGSVFPSLNMAVKRREQALQDYRRLQAKVEKYEEKEKTGPVLAKLHQAREELRPVRDDFEAKNKQLLDEMPRFYNSRLDYFQPSFESLIRAQVVYYSEMHKIFGDLTQQLDQPGHPDEQREQENEAKLSELRALSIVADD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MMB7</accession>
    <name>A0A8C4MMB7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zona occludens protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Zonula occludens protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">TJP1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Gap junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005028208.2">
        <property type="protein sequence ID" value="ENSEASP00005025984.2"/>
        <property type="gene ID" value="ENSEASG00005017725.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155164"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005921">
        <property type="term" value="C:gap junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045216">
        <property type="term" value="P:cell-cell junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090557">
        <property type="term" value="P:establishment of endothelial intestinal barrier"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905605">
        <property type="term" value="P:positive regulation of blood-brain barrier permeability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150105">
        <property type="term" value="P:protein localization to cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06727">
        <property type="entry name" value="PDZ1_ZO1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06728">
        <property type="entry name" value="PDZ2_ZO1-like_ds"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06729">
        <property type="entry name" value="PDZ3_ZO1-like_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12026">
        <property type="entry name" value="SH3_ZO-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000009">
        <property type="entry name" value="Putative tight junction protein ZO-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000013">
        <property type="entry name" value="Putative tight junction protein ZO-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.220.30:FF:000004">
        <property type="entry name" value="tight junction protein ZO-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000110">
        <property type="entry name" value="tight junction protein ZO-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000170">
        <property type="entry name" value="tight junction protein ZO-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005417">
        <property type="entry name" value="ZO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005418">
        <property type="entry name" value="ZO-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035597">
        <property type="entry name" value="ZO-1_SH3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000906">
        <property type="entry name" value="ZU5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13865">
        <property type="entry name" value="TIGHT JUNCTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13865:SF25">
        <property type="entry name" value="TIGHT JUNCTION PROTEIN ZO-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00791">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01597">
        <property type="entry name" value="ZONOCCLUDNS"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01598">
        <property type="entry name" value="ZONOCCLUDNS1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00218">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51145">
        <property type="entry name" value="ZU5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0303">Gap junction</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 15" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="5" id="KW-0796">Tight junction</keyword>
    <feature type="domain" description="PDZ" evidence="19">
        <location>
            <begin position="23"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="19">
        <location>
            <begin position="186"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="19">
        <location>
            <begin position="421"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="516"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="18">
        <location>
            <begin position="690"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="ZU5" evidence="20">
        <location>
            <begin position="1636"/>
            <end position="1770"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="103"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="296"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="825"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="988"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1043"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1096"/>
            <end position="1189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1229"/>
            <end position="1590"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="123"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="149"/>
            <end position="175"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="16">
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            <begin position="329"/>
            <end position="338"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="879"/>
            <end position="892"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="16">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
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        <location>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
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        <source>
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        <source>
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    <evidence type="ECO:0000256" key="5">
        <source>
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    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025984.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1770" mass="197674" checksum="9D648E1D548D670F" modified="2023-09-13" version="2">MSARAAAAKNTVMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSENEEDSYDEEVHDPRSGRGGLINRRSEKSWVRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLADSIHSANASERDDISEIQSLASDHSGRSHDRPPRHSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSREEERISKPGAISTPVKHADDHTPKTVEEVIVERNEKQAPTLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPSYSPQAQPQPIHRIDSPGFKTASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEELTPAPPSSYSPQADSLRTPSSEAAHIMLRDQEASLSSHVDPTKVYRKDPYPEDMMRQNHVLKQPAVGHPGQRADKEPNLSYEAQPPYVEKQASRDLEQPTYRYDSSSYAEQFSRNYDHRLHYEDRIPTYEEQWSYYDDKQPYQPRPSFDNQHPRDLDSRQHPEESSERGYFPRFEEPTPLSYDSRPRYDQPPRTSTLRHEEQAAPGYDIHNRYRPEAQPYSSAGPKASEAKQYFDQYPRSYEQVPSQGLTSKAGHYEPLHGAAVVTPLIPASQQKPEVLPSSTKPLPPPPTLTEEEEDPAMKPQSVLTRVKMFENKRSASLENKKDENHTTSFKPPEVASKPPGAPIIGPKATPQNQFSEHDKTLYRVPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPSPHIPASHLSEPAKPVPSQNQTNFSGYSLKGKSTEADALERSFGEKRYDPIQATPPPPPLPSQYTQPSQPGSSSSLALHTHAKGGHGEGNSASLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQSAFYPQKSFQDKAPINGAEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKPPTSPKTLVKAHSSAQPPEFDSGVETFSIHADKPKYQINNISTVPKAIPVSPSAMEEDEDEDGHTVVATARGVFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MNN4</accession>
    <name>A0A8C4MNN4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Testis expressed 50</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TEX50</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MNN4"/>
    <dbReference type="Ensembl" id="ENSEAST00005027254.2">
        <property type="protein sequence ID" value="ENSEASP00005025102.2"/>
        <property type="gene ID" value="ENSEASG00005017093.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00520000059630"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038833">
        <property type="entry name" value="TEX50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39232">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39232:SF1">
        <property type="entry name" value="TESTIS-EXPRESSED PROTEIN 50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040408685" evidence="3">
        <location>
            <begin position="24"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025102.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="176" mass="20604" checksum="887F7F12834CBE87" modified="2023-09-13" version="2">MSFQGLSLIFPFLFICFFKETFCVCDGTIWTKVGWEILPEEMQYLKFKPSPSYCLPYPLNNLCFNFVNMDIFQGYLYFTYTLVQALSFILFVLSVHYLWMKWKKHQKKLKKQPSLDTLGNDLEGPSFQDIDEILCRLMAKTSMLTNINQSSHYPLAKKVKHGKPKRKKTEGGGARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MR10</accession>
    <name>A0A8C4MR10_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger protein 132</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ZNF132</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MR10"/>
    <dbReference type="Ensembl" id="ENSEAST00005032312.2">
        <property type="protein sequence ID" value="ENSEASP00005029730.2"/>
        <property type="gene ID" value="ENSEASG00005020209.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163387"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000914">
        <property type="entry name" value="Zinc finger protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000352">
        <property type="entry name" value="zinc finger protein 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000127">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000443">
        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000519">
        <property type="entry name" value="Zinc finger protein 470"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000098">
        <property type="entry name" value="Zinc finger protein 614"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050636">
        <property type="entry name" value="C2H2-ZF_domain-containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772:SF15">
        <property type="entry name" value="REDUCED EXPRESSION 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47772">
        <property type="entry name" value="ZINC FINGER PROTEIN 200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="19"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="183"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="211"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="239"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="267"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="295"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="323"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="351"/>
            <end position="378"/>
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    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="379"/>
            <end position="406"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="13">
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            <begin position="407"/>
            <end position="434"/>
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    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="435"/>
            <end position="462"/>
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    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="463"/>
            <end position="490"/>
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    <feature type="domain" description="C2H2-type" evidence="13">
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            <begin position="491"/>
            <end position="518"/>
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            <end position="574"/>
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            <end position="630"/>
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        <location>
            <begin position="631"/>
            <end position="654"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    <evidence type="ECO:0000256" key="5">
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    </evidence>
    <evidence type="ECO:0000256" key="8">
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    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005029730.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="654" mass="75115" checksum="77C4FBB08F201783" modified="2023-09-13" version="2">MLENFELMTSLGCWHGVEDEEAHSKQTASVEGVSQVRIPSAHASTQKADTCDMCGPFLKDILHLDEHQGTHPEETPYTCGVCGREFWISAKLHQHQKEHSGEKPFIWDKGRNLFVKSSIVYLSEKLFTCGEDGKDVSDNHDLLQHPATDSSGHPCRSTECREAFPHSSGLGQLPEVHTTQKLFKCSACRKDFGKSSTLLNHLRTHSEGIPFRCSTVGNSLEKKSTLVNHQKFHTGEISYVCEECGKAFSHPSKLRKHQKFHTGVKYYKCSDCGKTFRHKLTLVHHQRVHTGERPYECSECGKAFNNRSHLTRHEKVHTGERPFECSKCGRAFSQSSNFLRHQKVHTQVRPYECNECGKAFSRSSALIQHWRVHTGERPYECSECGRAFNNNSNLAQHQKVHTGERPFECSECGRDFSQSSHLLRHQKVHTGERPFECSECGKAFSNSSTLIQHQKVHTGQRPYECSECRKAFSRSSSLIQHWRVHTGERPYECSECGKAFAHSSSLIEHWRIHTRERPYECSECGKFFSQNSILIKHQKVHTGERPYECSECGKFFSRKSSLIYHWRVHTGERPYECSECGRAFSNNSHLVRHQRVHTQERPYECSQCGKAFSERSTLVRHQIVHTKERTYECGHCGKIFSRLCNLAQHKKIHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MVC9</accession>
    <name>A0A8C4MVC9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Ankyrin repeat and IBR domain-containing protein 1</fullName>
            <ecNumber evidence="4">2.3.2.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ANKIB1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Might act as an E3 ubiquitin-protein ligase, or as part of E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes and then transfers it to substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 22">
            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.31"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RBR family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.31" evidence="4"/>
    <dbReference type="RefSeq" id="XP_014698430.1">
        <property type="nucleotide sequence ID" value="XM_014842944.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070364433.1">
        <property type="nucleotide sequence ID" value="XM_070508332.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070364442.1">
        <property type="nucleotide sequence ID" value="XM_070508341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MVC9"/>
    <dbReference type="Ensembl" id="ENSEAST00005032045.2">
        <property type="protein sequence ID" value="ENSEASP00005029486.1"/>
        <property type="gene ID" value="ENSEASG00005020038.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106832252"/>
    <dbReference type="KEGG" id="eai:106832252"/>
    <dbReference type="CTD" id="54467"/>
    <dbReference type="GeneTree" id="ENSGT00940000157621"/>
    <dbReference type="OMA" id="CNPENCC"/>
    <dbReference type="OrthoDB" id="69641at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20346">
        <property type="entry name" value="BRcat_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20361">
        <property type="entry name" value="Rcat_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16774">
        <property type="entry name" value="RING-HC_RBR_ANKIB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1750:FF:000003">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000040">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000129">
        <property type="entry name" value="RBR-type E3 ubiquitin transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045840">
        <property type="entry name" value="Ariadne"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031127">
        <property type="entry name" value="E3_UB_ligase_RBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002867">
        <property type="entry name" value="IBR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047564">
        <property type="entry name" value="Rcat_RBR_ANKIB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047563">
        <property type="entry name" value="RING-HC_RBR_ANKIB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044066">
        <property type="entry name" value="TRIAD_supradom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11685">
        <property type="entry name" value="RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19422">
        <property type="entry name" value="Ariadne"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01485">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22191">
        <property type="entry name" value="IBR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00647">
        <property type="entry name" value="IBR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51873">
        <property type="entry name" value="TRIAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50330">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="12 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="45"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="14">
        <location>
            <begin position="145"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="19">
        <location>
            <begin position="330"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="334"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="282"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="776"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="890"/>
            <end position="914"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="927"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1051"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="613"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="304"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="932"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="951"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1051"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1073"/>
            <end position="1085"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005029486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1092" mass="122558" checksum="AD62798DBDEC2AA8" modified="2022-01-19" version="1">MGNTTTKFRKALINGDENLACQIYENNPQLKESLDPNTSYGEPYQHNTPLHYAARHGMNKILRTFLFGRDGNPNKRNVHNETSMHLLCMGPQIMISEGALHPRLARPAEDDSRRADCLQMILRWKGAKLDQGEYERAAIDAIDNKKNTPLHYAAASGMRTCVELLVKHGGDLFAENENKDTPCDCAEKQHHKDLALNLESQMVFSRDPEAEEIEAEYAALDKREPYEGLRPQDLRKLKDMLIVETADMLQAPLFTAEALLRAHDWDREKLLEAWMSNPENCCQRSGVQMPTPPPSGYNAWDTLPSPRTPRTTRSSVTSPDEISLSPGDLDTSLCDICMCSISVFEDPVDMPCGHDFCRGCWESFLNLKIQEGEAHNIFCPAYDCFQLVPVDIIESVVSKEMDKRYLQFDIKAFVENNPAIKWCPTPGCERAVRLTKQGSNTSGSDTLSFPLLRAPAVDCGKGHLFCWECLGEAHEPCDCQTWKNWLQKITEMKPEELVGVSEAYEDAANCLWLLTNSKPCANCKSPIQKNEGCNHMQCAKCKYDFCWICLEEWKKHSSSTGGYYRCTRYEVIQHVEEQSKEMTVEAEKKHKRFQELDRFMHYYTRFKNHEHSYQLEQRLLKTAKEKMEQLSRALKETEGGCPDTTFIEDAVHVLLKTRRILKCSYPYGFFLEPKSTKKEIFELMQTDLEMVTEDLAQKVNRPYLRTPRHKIIKAACLVQQKRQEFLASVARGVAPADSPEAPRRSFAGGTWDWEYLGFASPEEYAEFQYRRRHRQQRRRGDVHSLLSNPPDPDEPSESTLDIPEGHSGRRPGPAVVSSASMSVLHSSSLRDYTPASRSENQDSLQALSSLDEDDPNILLAIQLSLQESGLAIDEESRDFLSNEASLGAIGTSLPSRLDSVPRNTDSPRAALSSSELLELGDSLMRLGAESDPFSTDTPSSHPLSEARSEFCPSSSDLDSAGQDANINDNLLGNIMAWFHDMNPQSIALIPPASTEISADSQHPYVKDGSEGVRGMELVPPEEDAMFEDAVISKGRGSQIEEENPLEENILAGEAASQAGESGNEAADKGDGSDVSSQTPQTSSEWLEQVHLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MVJ0</accession>
    <name>A0A8C4MVJ0_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">EMAP like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">EML1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat EMAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MVJ0"/>
    <dbReference type="Ensembl" id="ENSEAST00005034471.2">
        <property type="protein sequence ID" value="ENSEASP00005031711.2"/>
        <property type="gene ID" value="ENSEASG00005021584.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153887"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097431">
        <property type="term" value="C:mitotic spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002244">
        <property type="term" value="P:hematopoietic progenitor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007405">
        <property type="term" value="P:neuroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd21947">
        <property type="entry name" value="TD_EMAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000011">
        <property type="entry name" value="Echinoderm microtubule-associated protein-like 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000005">
        <property type="entry name" value="Putative echinoderm microtubule-associated protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055442">
        <property type="entry name" value="Beta-prop_EML-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055439">
        <property type="entry name" value="Beta-prop_EML_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005108">
        <property type="entry name" value="HELP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050630">
        <property type="entry name" value="WD_repeat_EMAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720:SF22">
        <property type="entry name" value="ECHINODERM MICROTUBULE-ASSOCIATED PROTEIN-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13720">
        <property type="entry name" value="WD-40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23409">
        <property type="entry name" value="Beta-prop_EML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23414">
        <property type="entry name" value="Beta-prop_EML_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03451">
        <property type="entry name" value="HELP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="EML-like first beta-propeller" evidence="11">
        <location>
            <begin position="284"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="559"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="domain" description="EML-like second beta-propeller" evidence="12">
        <location>
            <begin position="566"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="688"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="800"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="93"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="31"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="103"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="145"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005031711.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="835" mass="91998" checksum="9C7CE0A8CC15C5E8" modified="2023-09-13" version="2">MEDGFSSFSSLYDTSSLLQFCNDDSASAASSMEVTDRIASLEQRVQMQEDDIQLLKSALADVVRRLNITEEQQAVLNRKGPTKARPLVQTLPLRPTVNNGTVLPKRPSGSLPSPSGSRKETAVHAATKRLNRSLSPLSACKVHRSTSSTIKRTSSSERVSPGGRRESYGDSKGNRNRTGSTSSSSSGKKNSESKPKEPVFSAEEGYVKMFLRGRPVTMYMPKEQVDSYSLEAKAELPAKRLKLEWVYGYRGRDCRNNLYLLPTGETVYFIASVVVLYNVEEQLQRHYAGHNDDVKCLAVHPDRITIATGQVAGTSKDGKQLPPHVRIWDSVTLNTLHVIGIGFFDRAVTCIAFSKSNGGSNLCAVDDSNDHVLSVWDWQKEERLADVKCSNEAVFAADFHPTDTNIIVTCGKSHLYFWTLEGNSLIKKQGLFEKQEKPKFVLCVTFSENGDTITGDSSGNILVWGKGTNRISYAVQGAHEGGIFALCMLRDGTLVSGGGKDRKLISWNGNYQKLHKTEIPEQFGPIRTVAEGKGDVILIGTTRNFVLQGTLAGDFAPITQGHTDELWGLAIHASKPQFLTCGHDRHATLWDAVGHRPVWDKIIEDPAQSSGFHPSGSVVAVGTLTGRWFVFDTETKDLVTVHTDGNEQLSVMRYSPDGNFLAIGSHDNCIYIYGVSDNGRKYTRVGKCSGHSSFITHLDWSVNSQFLVSNSGDYEILYWIPSACKQVVSVETTRDIEWASHTCTLGFHVFGVWPEGSDGTDINAVCRAHEKKVLCTGDDFGKVHLFSYPCSQFRAPSHVYSGHSSHVTNVDFLCDDSHLISTGGKDTSIMQWRVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MW30</accession>
    <name>A0A8C4MW30_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Phosphodiesterase</fullName>
            <ecNumber evidence="19">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">PDE1A</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Calcium/calmodulin-dependent cyclic nucleotide phosphodiesterase with a dual specificity for the second messengers cGMP and cAMP, which are key regulators of many important physiological processes. Has a higher efficiency with cGMP compared to cAMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:25278"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3',5'-cyclic GMP + H2O = GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16957"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:16958"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a nucleoside 3',5'-cyclic phosphate + H2O = a nucleoside 5'-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14653"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57867"/>
            <dbReference type="ChEBI" id="CHEBI:58464"/>
            <dbReference type="EC" id="3.1.4.17"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:14654"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="19">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer. Interacts with YWHAZ.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4MW30"/>
    <dbReference type="Ensembl" id="ENSEAST00005035350.2">
        <property type="protein sequence ID" value="ENSEASP00005032458.2"/>
        <property type="gene ID" value="ENSEASG00005022135.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157043"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048101">
        <property type="term" value="F:calmodulin-activated 3',5'-cyclic-GMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004117">
        <property type="term" value="F:calmodulin-activated dual specificity 3',5'-cyclic-GMP, 3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000011">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013706">
        <property type="entry name" value="PDE1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08499">
        <property type="entry name" value="PDEase_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0112">Calmodulin-binding</keyword>
    <keyword evidence="10" id="KW-0114">cAMP</keyword>
    <keyword evidence="4" id="KW-0140">cGMP</keyword>
    <keyword evidence="6 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 18 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PDEase" evidence="21">
        <location>
            <begin position="167"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="530"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="535"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="16">
        <location>
            <position position="244"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <begin position="244"/>
            <end position="248"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="284"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="285"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="391"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010664"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="ARBA" id="ARBA00023149"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033675"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00033684"/>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
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        <source>
            <dbReference type="RuleBase" id="RU363067"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
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    </evidence>
    <sequence length="556" mass="63692" checksum="BEC4F6B18A524932" modified="2023-09-13" version="2">MCFSARTVTNELLLGRAGALSRRIIMGSTAADTEELENTTFKYLIGEQTEKMWQRLKGILRCLVKQLEKGDVNIVDLKKNIEYAASVLEAVYIDETRRLLDTEDELSDIQTDSVPSEVRDWLASTFTRKMGMKKKKSEEKPKFRSIVHAVQAGIFVERMYRKSYHVVGLAYPEAVIITLKDVDKWSFDVFALNEASGEHSLKFIIYELFTRYDLINRFKIPVSCLIAFAEALEVGYSKYKNPYHNLIHAADVTQTVHYIMLHTGIMHWLTELEILAMVFAAAIHDYEHTGTTNNFHIQTRSDVAILYNDRSVLENHHVSAAYRLMQEEEMNVLINLSKDDWRDLRNLVIEMVLSTDMSGHFQQIKNIRNSLQQPEGIDRAKTMSLILHAADISHPAKSWQLHYRWTMALMEEFFLQGDKEAELGLPFSPLCDRKSTMVAQSQIGFIDFIVEPTFSLLTDSTEKIIIPLIEEDSKTENPSYGASRRSNIKGTMNDGTYSPDYSLACVDLKSFKNNLVDIIQQNKERWKELAAQGESDLHKNSEDLANAEEKHADIHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MX04</accession>
    <name>A0A8C4MX04_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Interleukin-1 receptor-like 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">IL1RL1</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Inhibits IL-33 signaling.</text>
    </comment>
    <comment type="function">
        <text evidence="21">Receptor for interleukin-33 (IL-33) which plays crucial roles in innate and adaptive immunity, contributing to tissue homeostasis and responses to environmental stresses together with coreceptor IL1RAP. Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function. Upon tissue injury, induces UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the interleukin-1 receptor family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_044773058.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044628994.1">
        <property type="nucleotide sequence ID" value="XM_044773059.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070367658.1">
        <property type="nucleotide sequence ID" value="XM_070511557.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MX04"/>
    <dbReference type="Ensembl" id="ENSEAST00005035224.2">
        <property type="protein sequence ID" value="ENSEASP00005032344.2"/>
        <property type="gene ID" value="ENSEASG00005022045.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106839403"/>
    <dbReference type="CTD" id="9173"/>
    <dbReference type="GeneTree" id="ENSGT01090000259985"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004908">
        <property type="term" value="F:interleukin-1 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002113">
        <property type="term" value="F:interleukin-33 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002114">
        <property type="term" value="F:interleukin-33 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030225">
        <property type="term" value="P:macrophage differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002826">
        <property type="term" value="P:negative regulation of T-helper 1 type immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032689">
        <property type="term" value="P:negative regulation of type II interferon production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032722">
        <property type="term" value="P:positive regulation of chemokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050729">
        <property type="term" value="P:positive regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032754">
        <property type="term" value="P:positive regulation of interleukin-5 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043032">
        <property type="term" value="P:positive regulation of macrophage activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009611">
        <property type="term" value="P:response to wounding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd05757">
        <property type="entry name" value="Ig2_IL1R-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000002">
        <property type="entry name" value="Interleukin 1 receptor accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000188">
        <property type="entry name" value="Interleukin-1 receptor accessory protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000284">
        <property type="entry name" value="interleukin-1 receptor accessory protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001471">
        <property type="entry name" value="interleukin-1 receptor-like 1 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015621">
        <property type="entry name" value="IL-1_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004074">
        <property type="entry name" value="IL-1_rcpt_I/II-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041416">
        <property type="entry name" value="IL-1RAcP-like_ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013151">
        <property type="entry name" value="Immunoglobulin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890:SF7">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18452">
        <property type="entry name" value="Ig_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01536">
        <property type="entry name" value="INTRLKN1R12F"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="19" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="18" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="14 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 24" id="KW-0732">Signal</keyword>
    <keyword evidence="7 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="24">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040317235" description="Interleukin-1 receptor-like 1" evidence="24">
        <location>
            <begin position="19"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="330"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="26">
        <location>
            <begin position="28"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="26">
        <location>
            <begin position="114"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="26">
        <location>
            <begin position="212"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="25">
        <location>
            <begin position="375"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00053357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00053718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00069773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005032344.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="560" mass="63914" checksum="F008E2DC5B75E81D" modified="2023-09-13" version="2">MRQWVLAVLTFLISSTAAKFLKSSWGLENEALIVRCPRQGKSRYPVDWYYSETNTNILTEKRNHLLASGESLKFLPANVNDSGIYTCILRSPAANKTGYVNVTIYKKQPDCNIPDYLIYSKTSGSEKNSRIYCPTIDLYNWTAPVEWFKNCKVLQGSRYHTHSTFLLIDNATSKDAGDYTCKFMHSENGVSYSVTATRSFEVNDEEGFSLFPVIIAPPQNETKEVEIGKTASITCSACFGKGSQIMAAILWQVNRTKVGDFGEARIQEKQEQNQSSSNEMTCLNAVLRIADVKEEDLSLKYECWALNLHGSRNHIIRLRRKNPTEHQSTYYILAGFSILLMLIIVMMIMLKVFWIEVILLWRDIARPYKTRNDGKIYDAYVIYPRNHKNSPEGASSVEHFVHQILPDVLENKCGYNLCIYGRDLRPGEDAVTAVETNIQKSRRHIFILTPQITHSKEFAYEQEIALHSALIQNDSKVILIEMEALSKPGGLQFGELQDSLKHLVKVQGTIKWREGHVDNKRSLNSKFWKHVRYHMPVPNKPPRKTSRLASLSAHGQECLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N2X7</accession>
    <name>A0A8C4N2X7_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pejvakin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PJVK</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the gasdermin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4N2X7"/>
    <dbReference type="Ensembl" id="ENSEAST00005035752.2">
        <property type="protein sequence ID" value="ENSEASP00005032805.1"/>
        <property type="gene ID" value="ENSEASG00005022430.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183140"/>
    <dbReference type="OMA" id="IMNDVGF"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035253">
        <property type="term" value="C:ciliary rootlet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120044">
        <property type="term" value="C:stereocilium base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050910">
        <property type="term" value="P:detection of mechanical stimulus involved in sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000425">
        <property type="term" value="P:pexophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097468">
        <property type="term" value="P:programmed cell death in response to reactive oxygen species"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900063">
        <property type="term" value="P:regulation of peroxisome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120045">
        <property type="term" value="P:stereocilium maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040460">
        <property type="entry name" value="Gasdermin_pore"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042377">
        <property type="entry name" value="GSDME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15207">
        <property type="entry name" value="NONSYNDROMIC HEARING IMPAIRMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15207:SF2">
        <property type="entry name" value="PEJVAKIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04598">
        <property type="entry name" value="Gasdermin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gasdermin pore forming" evidence="4">
        <location>
            <begin position="1"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005032805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="352" mass="39982" checksum="60A40B0D96BDFC25" modified="2022-01-19" version="1">MFAAATKSFVKQVGDGGRLVPVPSLSEADKYQPLSLVVKKKRCFLFPRYKFTSTPFTLRDVLLGDREISAGISSYQLLNYEDESDVSLYGRRGNHIVNDVGINVAGSDSIAVKASFGIVTKHEVEVSTLLKEITTRKINFDHSLIRQSRSSRRAVLCVVMESIRTTRQCSLSVHAGIRGEAMRFHFMDEQNPKGRDKAIVFPAHTTIAFSVFELFIYLDGAFDLCVTSVSKGGFEREETATFALLYRLRNILFERNRRVMDAISRSQLYLDDLFSDYYDKPLRMTDISLKEGTHIRVNLLNHNIPKGPCILCGMGNFKRETVYGCFQCSVDGQKYVRLHAVPCFDVWHKRMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N429</accession>
    <name>A0A8C4N429_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nuclear cap-binding protein subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">80 kDa nuclear cap-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">NCBP1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the NCBP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014715232.2">
        <property type="nucleotide sequence ID" value="XM_014859746.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4N429"/>
    <dbReference type="SMR" id="A0A8C4N429"/>
    <dbReference type="Ensembl" id="ENSEAST00005036291.2">
        <property type="protein sequence ID" value="ENSEASP00005033281.2"/>
        <property type="gene ID" value="ENSEASG00005022795.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106842939"/>
    <dbReference type="KEGG" id="eai:106842939"/>
    <dbReference type="CTD" id="4686"/>
    <dbReference type="GeneTree" id="ENSGT00390000001733"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005846">
        <property type="term" value="C:nuclear cap binding complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006370">
        <property type="term" value="P:7-methylguanosine mRNA capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008334">
        <property type="term" value="P:histone mRNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042789">
        <property type="term" value="P:mRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030307">
        <property type="term" value="P:positive regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031442">
        <property type="term" value="P:positive regulation of mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048026">
        <property type="term" value="P:positive regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006408">
        <property type="term" value="P:snRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000021">
        <property type="entry name" value="Nuclear cap binding protein subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000010">
        <property type="entry name" value="Nuclear cap-binding protein subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027159">
        <property type="entry name" value="CBP80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015172">
        <property type="entry name" value="MIF4G-like_typ-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015174">
        <property type="entry name" value="MIF4G-like_typ-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412">
        <property type="entry name" value="CAP BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12412:SF2">
        <property type="entry name" value="NUCLEAR CAP-BINDING PROTEIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09088">
        <property type="entry name" value="MIF4G_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09090">
        <property type="entry name" value="MIF4G_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0506">mRNA capping</keyword>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="14" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="13" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="9" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MIF4G" evidence="18">
        <location>
            <begin position="28"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="666"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005033281.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="790" mass="91798" checksum="499EF27261DA789F" modified="2023-09-13" version="2">MSRRRHSDENDGGQPHKRRKTSDANETEDHLESLICKVGEKSACSLESNLEGLAGVLEADLPNYKSKILRLLCTVARLLPEKLTIYTTLVGLLNARNYNFGGEFVEAMIRQLKESLKANNYNEAVYLVRFLSDLVNCHVIAAPSMVAMFENFVSVTQEEDVPQVRRDWYVYAFLSSLPWVGKELYEKKDAEMDRIFANTESYLKRRQKTHVPMLQVWTAEKPHPQEEYLDCLWAQIQKLKKDRWQERHILRPYLAFDSILCEALQHNLPPFTPPPHTEDSVYPMPRVIFRMFDYTDDPEGPVMPGSHSVERFVIEENLHCIIKSHWKERKTCAAQLVSYPGKNKIPLNYHIVEVIFAELFQLPAPPHIDVMYTTLLIELCKLQPGSLPQVLAQATEMLYMRLDTMNTTCVDRFINWFSHHLSNFQFRWSWEDWSDCLTQDPESPKPKFVREVLEKCMRLSYHQRILDIVPPTFSALCPANPTCIYKYGDESSNSLPGHSVALCLAVAFKSKATNDEIFSILKDVPNPNQDDDDDEGFSFNPLKIEVFVQTLLHLAAKSFSHSFSALAKFHEVFKTLAESDEGKLHVLRVMFEVWRNHPQMIAVLVDKMIRTQIVDCAAVATWIFSSELSRDFTRLFVWEILHSTIRKMNKHVLKIQKELEEAKEKLARQHKRRSDDDDRSSDRKDGALEEQIERLQEKVESAQSEQKNLFLVIFQRFIMILTEHLVRCESDGTSVLTPWYKNCVERLQQIFLQHHQIIQQYMVTLENLLFTAELDPHILAVFQQFCALQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N8Q1</accession>
    <name>A0A8C4N8Q1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">BIRC7</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 20">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the IAP family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4N8Q1"/>
    <dbReference type="Ensembl" id="ENSEAST00005036965.2">
        <property type="protein sequence ID" value="ENSEASP00005033912.2"/>
        <property type="gene ID" value="ENSEASG00005023185.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160406"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043027">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity involved in apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010804">
        <property type="term" value="P:negative regulation of tumor necrosis factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
        <property type="term" value="P:positive regulation of protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042127">
        <property type="term" value="P:regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070247">
        <property type="term" value="P:regulation of natural killer cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00022">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16713">
        <property type="entry name" value="RING-HC_BIRC2_3_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000184">
        <property type="entry name" value="Baculoviral IAP repeat containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000002">
        <property type="entry name" value="Baculoviral IAP repeat containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000075">
        <property type="entry name" value="Baculoviral IAP repeat containing 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1170.10:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase XIAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1170.10">
        <property type="entry name" value="Inhibitor Of Apoptosis Protein (2mihbC-IAP-1), Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001370">
        <property type="entry name" value="BIR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050784">
        <property type="entry name" value="IAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10044:SF163">
        <property type="entry name" value="BACULOVIRAL IAP REPEAT-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10044">
        <property type="entry name" value="INHIBITOR OF APOPTOSIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00653">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00238">
        <property type="entry name" value="BIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57924">
        <property type="entry name" value="Inhibitor of apoptosis (IAP) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01282">
        <property type="entry name" value="BIR_REPEAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50143">
        <property type="entry name" value="BIR_REPEAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0789">Thiol protease inhibitor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="17">
        <location>
            <begin position="236"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="187"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="200"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005033912.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="282" mass="31125" checksum="D79AE70F1B42FB2E" modified="2023-09-13" version="2">MGPEDKAKCWCRGLELSHQAAGGSPMQGHHRPHCLCGCILGQDTGSALSWGGRDHVDGQILGQLHPLAEEEEEGAGAVAAPSTRPAFPRMGSEELRLASFCHWPLTAVVRPELLAAAGFFHTGWQDKVRCFFCHGGLQSWERGDDPWTEHAKWFPRCEFLLQTKGRDFVCRVQESCCHQLSSWDQLEEPEDAAPSAPVHRGPDPPMPRREAQSGAREPGAQNVEEQLQRLQEERTCKVCLDRAVSVVFVPCGHLVCAECAPNLQICPICRAPIDSCVRTFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4NAL9</accession>
    <name>A0A8C4NAL9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Recombining binding protein suppressor of hairless-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Transcription factor RBP-L</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RBPJL</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Putative transcription factor, which cooperates with EBNA2 to activate transcription.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts weakly with EBNA2. Does not interact with any Notch proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Su(H) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4NAL9"/>
    <dbReference type="Ensembl" id="ENSEAST00005037348.2">
        <property type="protein sequence ID" value="ENSEASP00005034263.2"/>
        <property type="gene ID" value="ENSEASG00005023396.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005197"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01176">
        <property type="entry name" value="IPT_RBP-Jkappa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1450:FF:000002">
        <property type="entry name" value="Recombination signal binding protein for immunoglobulin kappa J region like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000003">
        <property type="entry name" value="recombining binding protein suppressor of hairless"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000074">
        <property type="entry name" value="Recombining binding protein suppressor of hairless, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1450">
        <property type="entry name" value="LAG1, DNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015350">
        <property type="entry name" value="Beta-trefoil_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036358">
        <property type="entry name" value="BTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040159">
        <property type="entry name" value="CLS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015351">
        <property type="entry name" value="RBP-J/Cbf11/Cbf12_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037095">
        <property type="entry name" value="RBP-J/Cbf11_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038007">
        <property type="entry name" value="RBP-Jkappa_IPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10665">
        <property type="entry name" value="RECOMBINING BINDING PROTEIN SUPPRESSOR OF HAIRLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09270">
        <property type="entry name" value="BTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09271">
        <property type="entry name" value="LAG1-DNAbind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20144">
        <property type="entry name" value="TIG_SUH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01268">
        <property type="entry name" value="BTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01267">
        <property type="entry name" value="LAG1_DNAbind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110217">
        <property type="entry name" value="DNA-binding protein LAG-1 (CSL)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RBP-J/Cbf11/Cbf12 DNA binding" evidence="12">
        <location>
            <begin position="75"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-trefoil DNA-binding" evidence="13">
        <location>
            <begin position="214"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00069195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00080063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005034263.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="524" mass="56948" checksum="A78AD20BE2D06291" modified="2023-09-13" version="2">MDPVGAADPSAPPGPLTHLNLPDNSEARPQSGADGRSHPGSWTSRPAPTPRSSPEHATVLREGVRQCLQQQCQQTVWILHAKVAQKSYGNEKRFFCPPPCVYLAGPGWRVKPVQGQAHQAGETGPTVCGYMGLDGASGSAAETQKLNFEEQADSREFGCAKTLFISDADKRKHFRLVLRLVLRGGRELGTFHSRLIKVISKPSQKKQSLKNTDLCISSGSKVSLFNRLRSQTVSTRYLSVEDGAFVASARQWAAFTLHLADEHCSQGDFPPREGYVRYGSLVQLVCTVTGITLPPMIIRKVAKQCALLDVDEPISQLHKCAFQFPGGPPGGGGSYLCLATEKVVQFQASPCPKEANRALLNDSSCWTIIGTESVEFSFSASLACTREPVTPVPLISTLELSGGGDVATLELHGENFHAGLKVWFGNVEAETMYRSPRSLVCVVPDVAAFGSDWRWLRTPITVPVSLVRADGLFYPSAFSFTYTPEYSARPGPPAAPEPAADADALLESIHHEFTRTNFHLFIQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PFP9</accession>
    <name>A0A8C4PFP9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vesicle-associated membrane protein-associated protein A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">VAPA</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Endoplasmic reticulum (ER)-anchored protein that mediates the formation of contact sites between the ER and endosomes via interaction with FFAT motif-containing proteins such as STARD3 or WDR44. STARD3-VAPA interaction enables cholesterol transfer from the ER to endosomes. Via interaction with WDR44 participates in neosynthesized protein export. In addition, recruited to the plasma membrane through OSBPL3 binding. The OSBPL3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface. With OSBPL3, may regulate ER morphology. May play a role in vesicle trafficking.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044629388.1">
        <property type="nucleotide sequence ID" value="XM_044773453.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PFP9"/>
    <dbReference type="Ensembl" id="ENSEAST00005001731.2">
        <property type="protein sequence ID" value="ENSEASP00005001542.1"/>
        <property type="gene ID" value="ENSEASG00005001256.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106828651"/>
    <dbReference type="CTD" id="9218"/>
    <dbReference type="GeneTree" id="ENSGT00940000154799"/>
    <dbReference type="OMA" id="QKIDMME"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033149">
        <property type="term" value="F:FFAT motif binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000334">
        <property type="entry name" value="vesicle-associated membrane protein-associated protein A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000535">
        <property type="entry name" value="MSP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008962">
        <property type="entry name" value="PapD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016763">
        <property type="entry name" value="VAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10809:SF155">
        <property type="entry name" value="VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00635">
        <property type="entry name" value="Motile_Sperm"/>
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            <begin position="228"/>
            <end position="248"/>
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    <evidence type="ECO:0000256" key="1">
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00045917"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50202"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="249" mass="27857" checksum="93F57037F7FD0FED" modified="2022-01-19" version="1">MASASGAMAKHEQILVLDPPTDLKFKGPFTDVVTTNLKLRNPSDRKVCFKVKTTAPRRYCVRPNSGIIDPGLTVTVSVMLQPFDYDPNEKSKHKFMVQTIFAPPNTSDMEAVWKEAKPDELMDSKLRCVFEMPNENDKLNDIEPSKAVPVNASKQDGPMPKPHSVSLNDTETRKLMEECKRLQGEMMKLSEENRHLRDEGLRLRKVAHSDKPGSTSAASFRDNVTSPLPSLLVVIAAIFIGFFLGKFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PLX8</accession>
    <name>A0A8C4PLX8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
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        <name evidence="15" type="primary">LOC106844130</name>
    </gene>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="gene ID" value="ENSEASG00005010417.2"/>
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        <property type="component" value="Chromosome 17"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Olfactory receptor"/>
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    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="entry name" value="Olfact_rcpt"/>
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        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="27"/>
            <end position="49"/>
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    </feature>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="130"/>
            <end position="151"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="238"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="273"/>
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    </feature>
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        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="313" mass="34853" checksum="9EB6D4A7AAE8C6A0" modified="2022-01-19" version="1">MAPGNQTPVTEFILVGVSDRPDLQIPLFFVFLVIYGLTVAGNVGIITLTSVDSQLQTPMYFFLRHLAIINLGNSTVIAPKMLVNFLDSKKTISYYGCAAQLGGFLVFIVAEVFLLAAMAYDRYVAICNPLLYMVVVSPQICLLMVSLTYLYSLTTALTVSSCVFSVSYCSSNVINHFYCDNVPLLALSCSDTYIPETAVFTFSGTNLFFSMIIVLVSYFNIVLAVLRIRSSEGRQKAFSTCASHMMAVTVFYGTLLFMYLQPRTNHSLDTDKMASVFYTLVIPMMNPFIYSLRNKDVKDALKRFLITHVNCSN</sequence>
</entry>
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    <accession>A0A8C4PN85</accession>
    <name>A0A8C4PN85_EQUAS</name>
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        <submittedName>
            <fullName evidence="15">Zinc finger protein 879</fullName>
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        <name evidence="15" type="primary">ZNF879</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PN85"/>
    <dbReference type="Ensembl" id="ENSEAST00005019870.2">
        <property type="protein sequence ID" value="ENSEASP00005018306.1"/>
        <property type="gene ID" value="ENSEASG00005026494.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162816"/>
    <dbReference type="OMA" id="TCLGWES"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031519">
        <property type="term" value="C:PcG protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004137">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000824">
        <property type="entry name" value="Zinc finger protein 184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001100">
        <property type="entry name" value="Zinc finger protein 184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000944">
        <property type="entry name" value="zinc finger protein 232 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002129">
        <property type="entry name" value="Zinc finger protein 304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002402">
        <property type="entry name" value="Zinc finger protein 347"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000060">
        <property type="entry name" value="zinc finger protein 436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000416">
        <property type="entry name" value="zinc finger protein 879 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001162">
        <property type="entry name" value="zinc finger protein 879 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF21">
        <property type="entry name" value="ZINC FINGER PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="14">
        <location>
            <begin position="14"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="456"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="512"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="540"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005018306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="567" mass="65006" checksum="277224ACF72C9402" modified="2022-01-19" version="1">MAARWLPAQVQASVTFRDVAVLFSRDEWLRLDSAQRTLYREVMLENYSTLVSLGILFSKPKVIFQLQQGEDPWMMENGVSQGTCLGWESLFETTVSKEENQEIMNKLIDDGPCDFKLGKTYINEDKLEKQQGKKNKPFRKVLVTIQKPYMKERSFTGIELGKNLELKASLIRRPRILSRGRKPHSQQYTILFKQLGINTVRKCYKCNICGKIFLHSSSLSKHQRIHTGEKLYKCKECRKAFSQSSSLTQHLRVHTGEKPYICSECGKAFSFTTSLIGHQRMHTGERPYKCNECGKTFKGSSSLNNHQRIHTGEKPYKCNECGRAFSQCSSLIQHHRIHTGEKPYECSQCGKAFTSISRLSRHHRIHTGEKPFHCNECGKVFSYHSALIIHQRIHTGEKPYACKECGKAFSQSSALIQHQRIHTGEKPYKCNECGKAFSWISRLNIHNRIHTGEKPYNCKECGKAFSSHSAVNTHRKIHTGEKPYKCNDCEKAFNQSSALIQHQRIHTGEKPFNCKVCGKAFRQSSSLMTHMRIHTGEKPYKCKECGKAFSQSSSLTNHQRTHTGEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PPS4</accession>
    <name>A0A8C4PPS4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106834490</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cell membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014701611.1">
        <property type="nucleotide sequence ID" value="XM_014846125.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PPS4"/>
    <dbReference type="Ensembl" id="ENSEAST00005022748.2">
        <property type="protein sequence ID" value="ENSEASP00005020962.1"/>
        <property type="gene ID" value="ENSEASG00005014359.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106834490"/>
    <dbReference type="KEGG" id="eai:106834490"/>
    <dbReference type="GeneTree" id="ENSGT01090000260086"/>
    <dbReference type="OMA" id="LGSWICG"/>
    <dbReference type="OrthoDB" id="9444602at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13954">
        <property type="entry name" value="7tmA_OR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000010">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047132">
        <property type="entry name" value="Olfact_rcpt_6C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454:SF12">
        <property type="entry name" value="OLFACTORY RECEPTOR 6F1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="132"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363047"/>
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    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020962.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="308" mass="34196" checksum="741B48D89C72BCA7" modified="2022-01-19" version="1">MGTNNETLPHDFLLLGFPGSQALQLFLFMFFLVMYILTVSGNMAILMLVSTSHQLHTPMYFFLSNLSFLEIWYTTAAVPKALAILVGGCQTISFTSCLLQMYLVFSLGCTEYFLLAAMAYDRYLAICYPLHYGVIMNSFLSAQLALASWVCGFLAIAVPTALISNLTFCGPHTINHFFCDIAPWIALACTSTRGVELVAFVIAFVVILSSCLITLVSYIYIISTILRIPSASGRSKAFSTCSSHLAVVLIWYGSTIFLHVRTSIKEALDLTKAVHVLNTVVTPVLNPFIYTLRNKEVRETLLKKWKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PU56</accession>
    <name>A0A8C4PU56_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Klotho</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">KL</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PU56"/>
    <dbReference type="Ensembl" id="ENSEAST00005033719.2">
        <property type="protein sequence ID" value="ENSEASP00005031015.2"/>
        <property type="gene ID" value="ENSEASG00005021129.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157614"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017134">
        <property type="term" value="F:fibroblast growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005104">
        <property type="term" value="F:fibroblast growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055074">
        <property type="term" value="P:calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008340">
        <property type="term" value="P:determination of adult lifespan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006112">
        <property type="term" value="P:energy reserve metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030501">
        <property type="term" value="P:positive regulation of bone mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090080">
        <property type="term" value="P:positive regulation of MAPKKK cascade by fibroblast growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000042">
        <property type="entry name" value="Klotho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000062">
        <property type="entry name" value="Klotho"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033132">
        <property type="entry name" value="GH_1_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001360">
        <property type="entry name" value="Glyco_hydro_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10353:SF10">
        <property type="entry name" value="KLOTHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00232">
        <property type="entry name" value="Glyco_hydro_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00131">
        <property type="entry name" value="GLHYDRLASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00653">
        <property type="entry name" value="GLYCOSYL_HYDROL_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040107311" evidence="11">
        <location>
            <begin position="36"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="984"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="93"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005031015.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1014" mass="116178" checksum="653FBA0C6D349B94" modified="2023-09-13" version="2">MPARAPPRRPRPPPLPLPLPLLLLLLALGGRRLRAEPGDGAQTWARFARPPAPEAAGLLHGTFPDGFLWAVGSAAYQTEGGWRQHGKGASIWDTFTHHPPAPPGGPPLSGLPSGAPSPSPPATGDVASDGYNNVFRDTEGLRELGVTHYRFSISWARVLPNGSSGAPNREGLRYYRRLLERLRELGVQPVVTLYHWDLPQRLQDAYGGWANRALADHFRDYAELCFRHFGGQVKYWITIDNPYVVAWHGYATGRLAPGVRGSPQLGYLVAHNLLLAHAKIWHLYNTSFRPTQGGQVSIALSSHWINPRRMTDHSIKECQKSLDFVLGWFAKPIFIDGDYPETMKNNLSSLLPDFTESEKKFIKGTADFFALSFGPTLSFQLLDPHMKFRQLESPSLRQLLSWIDLEYNHPQIFIVENGWFVSGTTKRDDAKYMYYLKKFIMETLKAIRLDGVDVIGYTAWSLMDGFEWHRGYSIRRGLFYVDFLSQDKKLLPKSSALFYQKLIEKNGFPPLPENQPLEGTFPCDFAWGVVDNYIQVDTTLSQFTDPNVYLWDVHHSKRLIKVDGLVAKKRTSYCVDFAAIRPQIALLQEMHVSHFHFALDWPLILPLGNQSQVNRTGLHYYRCVVSELVRANITPVVALWRPSPQHQGLPRLLAKHGAWENPHTALAFAEYARLCFQELGHHVKFWITMSEPYTRNMTYSAGHNLLKAHALAWRVYDDKFRHAQKGKISIALQADWIEPACPFSQQDKEGAERVLEFDIGWLAEPIFGSGDYPPVMRDWLNQRNNFLLPYFSEEEKKLIRGSFDFLALSHYTTILVDWEKEDPMKYNDYLEVQEMTDITWLNSPSQVAVVPWGLRKVLNWLKSKYGDLPMYIIANGIDDDPQEAQDRLRVYYMQNYVNEALKAYVLDGINLCGYFAYSFNDRTAPKFGLYRYAANQFEPKPSMKHYRKIIDNNGFPGPKTLGRFCPEEFTLCTECSFFHTRKSLLAFIAFLLFAFIISLSLIFYYSKKGRRNYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I4F6</accession>
    <name>A0A9L0I4F6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106830147</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
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                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
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        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014839572.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A9L0I4F6"/>
    <dbReference type="Ensembl" id="ENSEAST00005074180.1">
        <property type="protein sequence ID" value="ENSEASP00005034823.1"/>
        <property type="gene ID" value="ENSEASG00005038053.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106830147"/>
    <dbReference type="KEGG" id="eai:106830147"/>
    <dbReference type="GeneTree" id="ENSGT01150000286987"/>
    <dbReference type="OrthoDB" id="5969463at2759"/>
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        <property type="component" value="Chromosome 20"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="CDD" id="cd15953">
        <property type="entry name" value="7tmA_OR52P-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000006">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
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    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF59">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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        <property type="entry name" value="OLFACTORYR"/>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8 11" id="KW-0297">G-protein coupled receptor</keyword>
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    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="60"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005034823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="317" mass="35347" checksum="237ADC535B583291" modified="2023-09-13" version="1">MADNSTHHYISSFFLVGIPGLQNFHCWIGLPVCLLFALTLLGNSVIIITIKLEPSLHQPMYFFLCMLAMNDMALASSTAPKMLGIFWLDAHRFDFNICLAQMYFIHTFCIIESALLVAMAFDRYVAICIPLRYTTVLTTTMVIKMGLAGVIRAILMVLPCPLLIKRLPYYTKHVINHAYCEHMAVVKLASANTLINRAYGISVALSVMVLDLGLIATSYIKILQAVFRLSSQNARSKALSTCAAHVCTILVSYTPALFSFLTHRIGKKVPPSIHIIFASLYLLVPPTVNPLVYGVKTKQIRDRVVGLFFPNKEISEN</sequence>
</entry>
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    <name>A0A9L0I6S4_EQUAS</name>
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        <recommendedName>
            <fullName evidence="3">Ras-GEF domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ras-like_GEF"/>
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        <property type="entry name" value="Ras_GEF_dom_sf"/>
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        <property type="entry name" value="RASGEF_cat_dom"/>
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    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
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        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR23113:SF35">
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ras-GEF" evidence="3">
        <location>
            <begin position="1"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005036014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="345" mass="38362" checksum="C244F76B702342CA" modified="2023-09-13" version="1">MTAQDRARVVELWIQVAKECRVLGNYASLRAIVSALQSPSISRLQKTWGRVARKSSRKLKRFIKDQWVSRRQLVKEATSMLTSLETGPIGAQKGLVPFLGTFLNYLLLLDTNMEDYLEGNEINYEKRSEVSSCGLPVRRVGLWQSETPPGKTFSGPMPWALLFLGEFGTQSWESRPIGAWGKGLAPRTPSCRRSYFKPTVRTSKSCTELEGRRVPMWAKTPDQLLDPLLTCLSPQHGTPPRPCLHHVLSPRNSKSPSRSSCSRRQSIFTTLRLRSDLGPGSRPWSSSARMRGEAGQELVRARRTLSDGQLQGARARGFRVSPGACCMVPRGALGLQLLAVSGRVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I760</accession>
    <name>A0A9L0I760_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="3 4">
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        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Mammalia</taxon>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="13" key="2">
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        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014697695.1">
        <property type="nucleotide sequence ID" value="XM_014842209.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0I9C1"/>
    <dbReference type="SMR" id="A0A9L0I9C1"/>
    <dbReference type="Ensembl" id="ENSEAST00005083266.1">
        <property type="protein sequence ID" value="ENSEASP00005036641.1"/>
        <property type="gene ID" value="ENSEASG00005025188.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106831792"/>
    <dbReference type="KEGG" id="eai:106831792"/>
    <dbReference type="CTD" id="7743"/>
    <dbReference type="GeneTree" id="ENSGT00940000161823"/>
    <dbReference type="OrthoDB" id="1095242at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002063">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001394">
        <property type="entry name" value="Zinc finger protein 189"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000631">
        <property type="entry name" value="zinc finger protein 189 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000352">
        <property type="entry name" value="zinc finger protein 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001697">
        <property type="entry name" value="zinc finger protein 623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000953">
        <property type="entry name" value="Zinc finger protein 691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000330">
        <property type="entry name" value="Zinc finger with KRAB and SCAN domains 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF106">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="16">
        <location>
            <begin position="14"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="288"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="456"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="512"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="540"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="568"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="599"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005036641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="626" mass="72921" checksum="327C4B462F21F6BE" modified="2023-09-13" version="1">MASPSPPPEPKGLLTFEDVAVFFTQEEWDYLDPAQRSLYKDVMMENYGNLVSLDVLNRDKDEEPTVKQEIEEIEEEVEPQGVIVTRIKSEIDQDPMGRETFELVGRLDKQRGIFLWEIPRESLTQEQRMFRGNTNIIRKRPTSEEKCHKCEECGKGFVRKAHFIQHQRVHTGEKPFQCNECGKSFSRSSFVIEHQRIHTGERPYECNYCGKTFSVSSTLIRHQRIHTGERPYQCNQCKQSFSQRRSLVKHQRIHTGEKPHKCSDCGKAFSWKSHLIEHQRTHTGEKPYHCTKCKKSFSRNSLLVEHQRIHTGERPHKCGECGKAFRLSTYLIQHQKIHTGEKPFLCIECGKSFSRSSFLIEHQRIHTGERPYQCKECGKSFSQLCNLTRHQRIHTGDKPHKCEECGKAFSRSSGLIQHQRIHTREKTYSYNETKESFDPNCSLVIQQEVYPKEKSYKCDECGKTFSVSAHLVQHQRIHTGEKPYLCTVCGKSFSRSSFLIEHQRIHTGERPYLCRQCGKSFSQLCNLIRHQGVHTGNKPHKCDECGKAFSRNSGLIQHQRIHTGEKPYKCEKCDKSFSQQRSLVNHQKIHAEVKTQETHECDACGEAFNCRISLLQHQKLHTTWMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IBF6</accession>
    <name>A0A9L0IBF6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial</fullName>
            <ecNumber evidence="13">6.2.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">GTP-specific succinyl-CoA synthetase subunit beta</fullName>
            <shortName evidence="13">G-SCS</shortName>
            <shortName evidence="13">GTPSCS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Succinyl-CoA synthetase beta-G chain</fullName>
            <shortName evidence="13">SCS-betaG</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 17" type="primary">SUCLG2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11 13">GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 13">
            <text>GTP + succinate + CoA = succinyl-CoA + GDP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22120"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="6.2.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="13">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for ATP. Interacts with ALAS2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12 13">Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the succinate/malate CoA ligase beta subunit family. GTP-specific subunit beta subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.4" evidence="13"/>
    <dbReference type="RefSeq" id="XP_044610338.1">
        <property type="nucleotide sequence ID" value="XM_044754403.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IBF6"/>
    <dbReference type="SMR" id="A0A9L0IBF6"/>
    <dbReference type="Ensembl" id="ENSEAST00005065824.1">
        <property type="protein sequence ID" value="ENSEASP00005035090.1"/>
        <property type="gene ID" value="ENSEASG00005001928.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106826210"/>
    <dbReference type="KEGG" id="eai:106826210"/>
    <dbReference type="CTD" id="8801"/>
    <dbReference type="GeneTree" id="ENSGT00390000010170"/>
    <dbReference type="OMA" id="KQMIGNR"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
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            <begin position="46"/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the SEC2 family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A9L0ID75"/>
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    <dbReference type="GeneTree" id="ENSGT00940000159102"/>
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        <property type="component" value="Chromosome 17"/>
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        <property type="term" value="C:Golgi to plasma membrane transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040351">
        <property type="entry name" value="RAB3IL/RAB3IP/Sec2"/>
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    <dbReference type="InterPro" id="IPR009449">
        <property type="entry name" value="Sec2_N"/>
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    <dbReference type="PANTHER" id="PTHR14430:SF5">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR FOR RAB-3A"/>
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        <property type="entry name" value="RABIN3-RELATED"/>
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        <property type="entry name" value="RAB3A-like_C"/>
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        <property type="entry name" value="Sec2 N-terminal region"/>
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    <keyword evidence="1 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="122"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="39"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="123"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="41"/>
            <end position="59"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF06428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005035725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="356" mass="39684" checksum="2C3B48EE323A6864" modified="2023-09-13" version="1">MDAPEKPSWCPARATCPVFLAMSSGTVCDAPTGLGLALEGNLREEPWDTESQPHLDEGHPPPLEAAPVSWKSVGPCKSLREPPGGLAEASVGEEAPGEEDPAAAQLDVLRLRSSSMEIREKGSEFLKEELHKAQKELKLKDEECERLSKVREQLEQELEELTASLFEEAHKMVREANMKQAASEKQLKEARGKVDTVLFAEFQAWRESPTLDKTCPFLERVYREDVGPCLDFTMQELSVLVRAAVEDNTLTIEPVASQTLPAMKVAAVECGSTNTCALSGLARACRHRIRLGDSEGHYYISPSSRARITAVCNFFTYIRYIQQGLVRQDAEPMFWEIMRLRKEMSLAKLGFFPQEA</sequence>
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    <name>A0A9L0IJ62_EQUAS</name>
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        <recommendedName>
            <fullName evidence="11">Enoyl reductase (ER) domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="9" type="primary">LOC106827760</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Zn(2+)</name>
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        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IJ62"/>
    <dbReference type="Ensembl" id="ENSEAST00005077811.1">
        <property type="protein sequence ID" value="ENSEASP00005040928.1"/>
        <property type="gene ID" value="ENSEASG00005036920.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162845"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004745">
        <property type="term" value="F:all-trans-retinol dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042573">
        <property type="term" value="P:retinoic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042572">
        <property type="term" value="P:retinol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000067">
        <property type="entry name" value="alcohol dehydrogenase 1-like isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000003">
        <property type="entry name" value="S-(hydroxymethyl)glutathione dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43880:SF6">
        <property type="entry name" value="ALCOHOL DEHYDROGENASE 6A (CLASS V)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="8">
        <location>
            <begin position="49"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="7">
        <location>
            <begin position="218"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005040928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="384" mass="40993" checksum="7F010B2CA07D7FC4" modified="2023-09-13" version="1">MECDDNIQCFPTCGNSWTLSPTAGKINAIAWKRNSPLSIEEVQLEPPKAGEVRIKMTSSAICGSDNHILKGSLPGNFPLIPGHEGAGIVESTGEGVSSVKPGDKVLTLVVPQCRECNSCLAPKGNFCEKQDILPSSGLMLDGTSRFTCKGQEIYQAFRTSTFAEYTVVPEIAVAKIDDAAPMDKVCLLSCGMPAGYGAAVHSAKVTPGSTCVVFGLGGIGSAVVMACKASGASRIIGVDINEEKFPRARVLGVTDCLNPWKLKEPVQQVVVEMTGFGVDFAFEAIGLVETVVYCCLESCHLSYGICQIIGLAPSNSQLSLEALMVVSGRTLKGVCLGGYKTRDCIPQLVTDYLQNKINLDPLITHQLPFDQLHKAFKLYHAGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IK33</accession>
    <name>A0A9L0IK33_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">PBX homeobox 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">PBX3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="J."/>
                <person name="Fang"/>
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                <person name="S."/>
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                <person name="Y."/>
                <person name="Liu"/>
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                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/PBX homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044634690.1">
        <property type="nucleotide sequence ID" value="XM_044778755.2"/>
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    <dbReference type="AlphaFoldDB" id="A0A9L0IK33"/>
    <dbReference type="SMR" id="A0A9L0IK33"/>
    <dbReference type="Ensembl" id="ENSEAST00005048644.1">
        <property type="protein sequence ID" value="ENSEASP00005038130.1"/>
        <property type="gene ID" value="ENSEASG00005020621.2"/>
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    <dbReference type="GeneID" id="106830830"/>
    <dbReference type="KEGG" id="eai:106830830"/>
    <dbReference type="CTD" id="5090"/>
    <dbReference type="GeneTree" id="ENSGT00940000154374"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0008344">
        <property type="term" value="P:adult locomotory behavior"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0021516">
        <property type="term" value="P:dorsal spinal cord development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002087">
        <property type="term" value="P:regulation of respiratory gaseous exchange by nervous system process"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0007585">
        <property type="term" value="P:respiratory gaseous exchange by respiratory system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000008">
        <property type="entry name" value="Pre-B-cell leukemia transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
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    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
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    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005542">
        <property type="entry name" value="PBX_PBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03792">
        <property type="entry name" value="PBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51978">
        <property type="entry name" value="PBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="PBC" evidence="11">
        <location>
            <begin position="41"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="233"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
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            <begin position="235"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="20"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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    </feature>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
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            <begin position="32"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
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            <begin position="326"/>
            <end position="341"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
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            <begin position="403"/>
            <end position="422"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007601"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51978"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="434" mass="47190" checksum="81987E4C80C22069" modified="2023-09-13" version="1">MDDQSRMLQTLAGVNLAGHSVQGGMALPPPPHGHEGADGDGRKQDIGDILHQIMTITDQSLDEAQAKKHALNCHRMKPALFSVLCEIKEKTGLSIRGAQEEDPPDPQLMRLDNMLLAEGVSGPEKGGGSAAAAAAAAASGGSSDNSIEHSDYRAKLTQIRQIYHTELEKYEQACNEFTTHVMNLLREQSRTRPISPKEIERMVGIIHRKFSSIQMQLKQSTCEAVMILRSRFLDARRKRRNFSKQATEILNEYFYSHLSNPYPSEEAKEELAKKCSITVSQVSNWFGNKRIRYKKNIGKFQEEANLYAAKTAVTAAHAVAAAVQNNQTNSPTTPNSGSSGSFNLPNSGDMFMNMQSLNGDSYQGSQVGANVQSQVDTLRHVINQTGGYSDGLGGNSLYSPHNLNANGGWQDATTPSSVTSPTEGPGSVHSDTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IKQ8</accession>
    <name>A0A9L0IKQ8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Zinc finger protein 688</fullName>
        </submittedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSEASP00005038365.1"/>
        <property type="gene ID" value="ENSEASG00005029621.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162549"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000180">
        <property type="entry name" value="Zinc finger protein 689"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF69">
        <property type="entry name" value="ZINC FINGER PROTEIN 688"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="8">
        <location>
            <begin position="26"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="145"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="173"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="63"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="198"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="89"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="238" mass="26499" checksum="2A1BFF8BED3A37F5" modified="2023-09-13" version="1">MAPPPAPLLALRPGETLPGCNKPVAVRFADVAVYFSPEEWGCLRPAQRALYRDVMRETYGHLGALGEAPNKKDEEPEEGRGAKGPRKTSGKERPKEPIKHNLDSTISKASARAQPPTKGAWDQSAGVQPPIPMPSMDAPSSQRRHVCADCGRRFTYPSLLVSHRRMHSGERPFPCPECGMRFKRKFAVEAHQWIHRSCSGGRRGRRPGIRAVPRAPVRGDRDPPVLFRHYPDIFEECG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0ILW5</accession>
    <name>A0A9L0ILW5_EQUAS</name>
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        <submittedName>
            <fullName evidence="5">Ankyrin repeat domain 49</fullName>
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        <name evidence="5" type="primary">ANKRD49</name>
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    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_014835396.3"/>
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        <property type="nucleotide sequence ID" value="XM_014835397.3"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_044752118.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_070490433.1"/>
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        <property type="protein sequence ID" value="ENSEASP00005041945.1"/>
        <property type="gene ID" value="ENSEASG00005032651.1"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00390000003919"/>
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        <property type="component" value="Chromosome 20"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
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        <property type="entry name" value="Lethal (2) 35Be"/>
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        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Ankyrin_rpt"/>
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    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
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    <dbReference type="PANTHER" id="PTHR24198">
        <property type="entry name" value="ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24198:SF165">
        <property type="entry name" value="ANKYRIN REPEAT-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="106"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="139"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005041945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="238" mass="27137" checksum="FC3651CA0561B8D1" modified="2023-09-13" version="1">MEKEKVNDDGKPDSEDSLDFSEHFNQLELLETHGHLIPTGTQSLWEENSDEDDQDEKTEEWYQLQEKKMGKDPSKLLLWAAEKNRLATVQRLLSEKATHVNTRDEDEYTPLHRAAYGGHLDVVRELIAQGADVHAVTVDGWTPLHSACKWNNTRVASFLLQHDADINAQTKGLLTPLHLAAGNRDSKDTLELLLMNRYIKPGLKNNLEETAFDIARRTSIYHYLFEIVEDCTNSSPQS</sequence>
</entry>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="Sun"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Yang"/>
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                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
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                <person name="Yin"/>
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                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
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        <property type="nucleotide sequence ID" value="XM_070503915.1"/>
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        <property type="gene ID" value="ENSEASG00005031250.1"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00390000012351"/>
    <dbReference type="OrthoDB" id="8754475at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
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    <dbReference type="GO" id="GO:0005856">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="InterPro" id="IPR028021">
        <property type="entry name" value="Katanin_C-terminal"/>
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    <dbReference type="InterPro" id="IPR042404">
        <property type="entry name" value="KATNBL1"/>
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    <dbReference type="PANTHER" id="PTHR14682">
        <property type="entry name" value="KATNB1-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14682:SF1">
        <property type="entry name" value="KATNB1-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13925">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Katanin p80 subunit C-terminal" evidence="5">
        <location>
            <begin position="149"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005042404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="304" mass="34855" checksum="2EAE58AFA4D89B8D" modified="2023-09-13" version="1">MASETHNVKKRNFCNNIEDHYIDLPRKRISKFTNKNMKEVKKSPKHLAAYINRTVGQAVKSPDKLRKVIYHRKKVHHSSPNTCYRKKQSPRSGGCDMANKENELACAGHLPEKLRHDSRTYLVNSSDSSSSQTESPSSKYGGFFSEVSQDHETMAQVLFSRNLRLNVALTFWRKRSISELVAYLVRIEDLGVVVDCLPVLTNSLQEEKQYISLGCCVDLLPLVKSLLKSKFEEYIIVGLNWLQAVIKRWWSELSSKTEIRNDGNIQILKQQLSGLWEQEHHLTLVPGYTGNIAKDVDAYLLQLH</sequence>
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    <name>A0A9L0INW7_EQUAS</name>
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            <fullName evidence="5">2'-5' oligoadenylate synthase</fullName>
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            <taxon>Mammalia</taxon>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
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    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3 ATP = 5'-triphosphoadenylyl-(2'-&gt;5')-adenylyl-(2'-&gt;5')-adenosine + 2 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:34407"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:67143"/>
            <dbReference type="EC" id="2.7.7.84"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the 2-5A synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.84" evidence="5"/>
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    <dbReference type="Ensembl" id="ENSEAST00005080106.1">
        <property type="protein sequence ID" value="ENSEASP00005038778.1"/>
        <property type="gene ID" value="ENSEASG00005009520.2"/>
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    <dbReference type="GeneTree" id="ENSGT00510000046406"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0001730">
        <property type="term" value="F:2'-5'-oligoadenylate synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0045071">
        <property type="term" value="P:negative regulation of viral genome replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05400">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.20:FF:000001">
        <property type="entry name" value="2'-5'-oligoadenylate synthetase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000007">
        <property type="entry name" value="2'-5'-oligoadenylate synthetase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.20">
        <property type="entry name" value="2'-5'-oligoadenylate synthetase 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018952">
        <property type="entry name" value="2-5-oligoAdlate_synth_1_dom2/C"/>
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    <dbReference type="InterPro" id="IPR006117">
        <property type="entry name" value="2-5OAS_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043518">
        <property type="entry name" value="2-5OAS_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006116">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11258:SF13">
        <property type="entry name" value="2'-5'-OLIGOADENYLATE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11258">
        <property type="entry name" value="2-5 OLIGOADENYLATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10421">
        <property type="entry name" value="OAS1_C"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
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    <dbReference type="PROSITE" id="PS00832">
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    <dbReference type="PROSITE" id="PS50152">
        <property type="entry name" value="25A_SYNTH_3"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0051">Antiviral defense</keyword>
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    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0399">Innate immunity</keyword>
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    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
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            <end position="106"/>
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            <end position="348"/>
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            <end position="392"/>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
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            <dbReference type="Pfam" id="PF10421"/>
        </source>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
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</entry>
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        <submittedName>
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
        </lineage>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="H."/>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Guo"/>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
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                <person name="Li"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
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                <person name="Hou"/>
                <person name="M."/>
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                <person name="Jin"/>
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                <person name="M."/>
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                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
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                <person name="X. and Zhong"/>
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    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
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    </reference>
    <comment type="subcellular location">
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            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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        <property type="component" value="Chromosome 14"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="HIRIP3"/>
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    <dbReference type="InterPro" id="IPR019098">
        <property type="entry name" value="Histone_chaperone_domain_CHZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15410">
        <property type="entry name" value="HIRA-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15410:SF2">
        <property type="entry name" value="HIRA-INTERACTING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09649">
        <property type="entry name" value="CHZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01082">
        <property type="entry name" value="CHZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone chaperone" evidence="5">
        <location>
            <begin position="511"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="75"/>
            <end position="451"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="530"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="557"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="103"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="212"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="245"/>
            <end position="259"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="583" mass="65109" checksum="3A04E3EDF6B0E5C5" modified="2023-09-13" version="1">MARENEMQEFARSFFRGRPDLSTLTHSIVRRRFLAHTGRDHLEPEEKQALKRLVEEELLKMQVWVDQTGAREERLGLAKKVKRPPIPCSDSERKRFRFDSESEPSSAASSPDCFGSPAKNGMAAAISSAKEESPKQASKKAVESSDEEEQQRDPTAKVGLEREEVKETSEEEEEEGSARTSKVWKEESSEEEEEEEEEKEAKGRTRKKLVTKNKQAPSKASASRKQAREESEDSEEEPAQRMRKKAEGEKGAKSEKESEKESEEEETLAKKKENREEEEEDWKPRAWDNGGKRSAWEERRYKQKSRAARLLGDLGDQEEEKEKAAAGSGNKSRGDDEPPVQRKSKDKSQWKGGKRQSGSSEDGEDSGRKMKPTAKGTGKTANMGGINGEASDSEREVTDSEAGGSPKGERKNRSSKKSSKKDRTQSSSSADGSPEPKGRKAGSGCRGEDHPAVRKLKRYIRACGAHRNYKKLLGSCRSYKECLSVLRAELEALGMKGNPSLEKCRALKEQREEAAEVASLDITNIISSSGRPRRRTAWNPSGEAAPPGELYCRTLDSEEEQPHLPPPDWSHLRGIISSDGESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IPP3</accession>
    <name>A0A9L0IPP3_EQUAS</name>
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        <submittedName>
            <fullName evidence="10">Cytohesin 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CYTH4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
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        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
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    <dbReference type="Ensembl" id="ENSEAST00005058850.1">
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        <property type="gene ID" value="ENSEASG00005018847.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837586"/>
    <dbReference type="CTD" id="27128"/>
    <dbReference type="GeneTree" id="ENSGT00940000160865"/>
    <dbReference type="OMA" id="LYNPNCK"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01252">
        <property type="entry name" value="PH_GRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000002">
        <property type="entry name" value="Cytohesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.20:FF:000003">
        <property type="entry name" value="Cytohesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cytohesin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF323">
        <property type="entry name" value="CYTOHESIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="5" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="9">
        <location>
            <begin position="54"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="8">
        <location>
            <begin position="259"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="19"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
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            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="394" mass="45522" checksum="2A8FBB230BA1F7BB" modified="2023-09-13" version="1">MDLCHPDPTELSSGEAEELQRIKWHRKQLLEDIQKLKDEIADVFAQIDCFETAEESRMAQKEKELCIGRKKFNMDPVKGIQYLTEHKLLSPDVQDIAQFLYKGEGLNKTAIGTYLGERDPVNLQVLQAFVDCHEFANLNLVQALRQFLWSFRLPGEAQKIDRMMETFATRYCLCNPGVFQSTDTCYVLSFSIIMLNTSLHNPNVRDRPPFERFVSMNRGINGGSDLPEEQLRNLFDSIKSEPFSIPEDDGNDLTHTFFKPDREGWLLKLGGRVKTWKRRWFILTDNCLYYFEFTTDKEPRGIIPLENLSVQKVEDPKKPFCLELYNPSCRGQKIKACKTDGDGKVVEGKHESYRISASSAEERDQWIEAIQASITRDPFYDLVSARKKKIASKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IS13</accession>
    <name>A0A9L0IS13_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier organic anion transporter family member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SLCO2B1</name>
    </gene>
    <organism evidence="11 12">
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the organo anion transporter (TC 2.A.60) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014711353.1">
        <property type="nucleotide sequence ID" value="XM_014855867.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IS13"/>
    <dbReference type="Ensembl" id="ENSEAST00005052727.1">
        <property type="protein sequence ID" value="ENSEASP00005042908.1"/>
        <property type="gene ID" value="ENSEASG00005014263.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106840411"/>
    <dbReference type="KEGG" id="eai:106840411"/>
    <dbReference type="CTD" id="11309"/>
    <dbReference type="GeneTree" id="ENSGT01150000286901"/>
    <dbReference type="OMA" id="FMLGSAM"/>
    <dbReference type="OrthoDB" id="5062115at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015125">
        <property type="term" value="F:bile acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015132">
        <property type="term" value="F:prostaglandin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015347">
        <property type="term" value="F:sodium-independent organic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="KAZAL_2"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
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    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="89"/>
            <end position="110"/>
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</entry>
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    <name>A0A9L0IW14_EQUAS</name>
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        <recommendedName>
            <fullName evidence="2">Single-stranded DNA-binding protein, mitochondrial</fullName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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                <person name="Liu"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Li"/>
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                <person name="Zhang"/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="M."/>
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                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="5">Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays a crucial role during mtDNA replication by stimulating the activity of the replisome components POLG and TWNK at the replication fork. Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity. In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK. May also function in mtDNA repair.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer. Interacts with MPG/AAG, through inhibition of its glycosylase activity it potentially prevents formation of DNA breaks in ssDNA, ensuring that base removal primarily occurs in dsDNA. Interacts with POLDIP2. Interacts with PRIMPOL.</text>
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        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
            <location evidence="1">Mitochondrion nucleoid</location>
        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="ENSEASG00005015589.2"/>
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        <property type="component" value="Chromosome 16"/>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
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        <property type="entry name" value="NA-bd_OB-fold"/>
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        <property type="entry name" value="Primosome_PriB/ssb"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SINGLE-STRANDED DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SINGLE-STRANDED DNA-BINDING PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1135">Mitochondrion nucleoid</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019399"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023271"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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    <accession>A0A9L0IW93</accession>
    <name>A0A9L0IW93_EQUAS</name>
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        <submittedName>
            <fullName evidence="1">Organic solute carrier partner 1</fullName>
        </submittedName>
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    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Fan"/>
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                <person name="Li"/>
                <person name="J."/>
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                <person name="Wu"/>
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                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <scope>IDENTIFICATION</scope>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <location>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    <sequence length="163" mass="18365" checksum="D9C09937D95981B2" modified="2023-09-13" version="1">MECFELTNQRRGRGRWVGRSAPTPGSSCLGSAGDRISRHLSLLVVQSPVLSSRDLERPQCPRKDSGDPGALEMDSLAIEDVAVNFTPEEWALLDPSQKKLYRDVMWETFRHLASVGITWEHHDIKDQCKDKGTKLRIVLHEGDRRVLDCQDSQSLLFHPCAGL</sequence>
</entry>
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    <accession>A0A9L0IXR0</accession>
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            <fullName evidence="11">Solute carrier family 10 member 5</fullName>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="11" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <text evidence="2">Belongs to the bile acid:sodium symporter (BASS) (TC 2.A.28) family.</text>
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        <property type="entry name" value="Bilac:Na_transpt"/>
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        <property type="entry name" value="Na+/solute_symporter_sf"/>
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        <property type="entry name" value="NTCP5_P3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361">
        <property type="entry name" value="SODIUM-BILE ACID COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10361:SF29">
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    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="1"/>
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        </location>
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    <feature type="chain" id="PRO_5044699417" evidence="9">
        <location>
            <begin position="20"/>
            <end position="437"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="143"/>
            <end position="164"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="176"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="34"/>
            <end position="124"/>
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        <source>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005042547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="437" mass="48875" checksum="179C007E041D03CE" modified="2023-09-13" version="1">MSRKLFIVLLLLFVTLGEARKSFLSFLTIEKTEILFFTKTEETIIVRSSYKDKWPNSSYLFVQLEDPKMLRVVNVTKTLSDVTNFTINLVTDEVGETNLTIQLWDSEGRQERLIEEIKNVKVKVLRQRKDDLFQASMRIDRNILMIFLPLILLNKCAFGCKIELEVFQTVWKKPLPIILGAVTQFFLMPFCGFLLTQILALPEAQAFGFIMTCTCPGGGGGYLFALLVGGDITLAILMTCTSTLLALIMMPANSYIYSRMLGLSGTFHIPVSKITSTLLFILIPISAGIVIKRRKPEKAKLLERIIRPLSFILMSVGIYMTFRIGLMFLKTVSLEVLLLGALVPTLGLSFGYSFATISMLPLPVCKTVAIESGILNSFLALAIIQLSFSQSKANLASVAPFTVAMCSGCEMLVILLVYKARKTWILIEDKRKNIPLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J329</accession>
    <name>A0A9L0J329_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Glutathione peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GPX1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + 2 glutathione = (12S)-hydroxy-(5Z,8Z,10E,14Z)-eicosatetraenoate + glutathione disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:50708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57444"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="ChEBI" id="CHEBI:90680"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:50709"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>2 glutathione + H2O2 = glutathione disulfide + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:16833"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58297"/>
            <dbReference type="EC" id="1.11.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:16834"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotetramer. Interacts with MIEN1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the glutathione peroxidase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J329"/>
    <dbReference type="Ensembl" id="ENSEAST00005055907.1">
        <property type="protein sequence ID" value="ENSEASP00005047676.1"/>
        <property type="gene ID" value="ENSEASG00005036373.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156150"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097413">
        <property type="term" value="C:Lewy body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004602">
        <property type="term" value="F:glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047066">
        <property type="term" value="F:phospholipid-hydroperoxide glutathione peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990782">
        <property type="term" value="F:protein tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060055">
        <property type="term" value="P:angiogenesis involved in wound healing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051702">
        <property type="term" value="P:biological process involved in interaction with symbiont"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043534">
        <property type="term" value="P:blood vessel endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001885">
        <property type="term" value="P:endothelial cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045444">
        <property type="term" value="P:fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048144">
        <property type="term" value="P:fibroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060047">
        <property type="term" value="P:heart contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008631">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to oxidative stress"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019372">
        <property type="term" value="P:lipoxygenase pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045445">
        <property type="term" value="P:myoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051450">
        <property type="term" value="P:myoblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902042">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway via death domain receptors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002862">
        <property type="term" value="P:negative regulation of inflammatory response to antigenic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902176">
        <property type="term" value="P:negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090201">
        <property type="term" value="P:negative regulation of release of cytochrome c from mitochondria"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051402">
        <property type="term" value="P:neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033599">
        <property type="term" value="P:regulation of mammary gland epithelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061136">
        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010332">
        <property type="term" value="P:response to gamma radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033194">
        <property type="term" value="P:response to hydroperoxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032496">
        <property type="term" value="P:response to lipopolysaccharide"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010269">
        <property type="term" value="P:response to selenium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009609">
        <property type="term" value="P:response to symbiotic bacterium"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048741">
        <property type="term" value="P:skeletal muscle fiber development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043403">
        <property type="term" value="P:skeletal muscle tissue regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001659">
        <property type="term" value="P:temperature homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009650">
        <property type="term" value="P:UV protection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042311">
        <property type="term" value="P:vasodilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00340">
        <property type="entry name" value="GSH_Peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000153">
        <property type="entry name" value="Glutathione peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000889">
        <property type="entry name" value="Glutathione_peroxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029760">
        <property type="entry name" value="GPX_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11592:SF41">
        <property type="entry name" value="GLUTATHIONE PEROXIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00255">
        <property type="entry name" value="GSHPx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000303">
        <property type="entry name" value="Glutathion_perox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01011">
        <property type="entry name" value="GLUTPROXDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00763">
        <property type="entry name" value="GLUTATHIONE_PEROXID_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51355">
        <property type="entry name" value="GLUTATHIONE_PEROXID_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2 8" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0712">Selenocysteine</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005047676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="208" mass="23163" checksum="B6E77488220CE685" modified="2023-09-13" version="1">KKKWKRLSCVTSKYMRGFVFLSQAMCAAQLAAAARRSVYAFSARPLAGGEPLSLGTTVRDYTQMNELQRRLGPRGLVVLGFPCNQFGHQENAKNEEILNSLKYVRPGGGFEPNFTLFEKCEVNGAQAHPLFAFLREALPAPSDDATALMTDPKFITWSPVCRNDVAWNFEKFLVGPDGVPVRRYSRRFPTIDIEPDIEALLTQGPSCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J5F0</accession>
    <name>A0A9L0J5F0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 24 25">Phosphatidate cytidylyltransferase</fullName>
            <ecNumber evidence="14 24 25">2.7.7.41</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">CDS2</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Catalyzes the conversion of phosphatidic acid (PA) to CDP-diacylglycerol (CDP-DAG), an essential intermediate in the synthesis of phosphatidylglycerol, cardiolipin and phosphatidylinositol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>1,2-di-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-sn-glycero-3-phosphate + CTP + H(+) = 1,2-di-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45656"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:77126"/>
            <dbReference type="ChEBI" id="CHEBI:85351"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:45657"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1,2-di-(9Z,12Z-octadecadienoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45672"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:77128"/>
            <dbReference type="ChEBI" id="CHEBI:85355"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:45673"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45676"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
            <dbReference type="ChEBI" id="CHEBI:85356"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:45677"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45652"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:72864"/>
            <dbReference type="ChEBI" id="CHEBI:85350"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:45653"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1-octadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1-octadecanoyl-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45668"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:77130"/>
            <dbReference type="ChEBI" id="CHEBI:85354"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="2">
            <dbReference type="Rhea" id="RHEA:45669"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45648"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:77091"/>
            <dbReference type="ChEBI" id="CHEBI:85349"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:45649"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>1-octadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1-octadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45660"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:77098"/>
            <dbReference type="ChEBI" id="CHEBI:85352"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:45661"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphate + CTP + H(+) = 1-octadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-cytidine-5'-diphosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:45664"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74560"/>
            <dbReference type="ChEBI" id="CHEBI:85353"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:45665"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1,2-diacyl-sn-glycero-3-phosphate + CTP + H(+) = a CDP-1,2-diacyl-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:16229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="EC" id="2.7.7.41"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:16230"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="12">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11 24 25">Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 24 25">Belongs to the CDS family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.41" evidence="14 24 25"/>
    <dbReference type="RefSeq" id="XP_044604531.1">
        <property type="nucleotide sequence ID" value="XM_044748596.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J5F0"/>
    <dbReference type="Ensembl" id="ENSEAST00005073139.1">
        <property type="protein sequence ID" value="ENSEASP00005048511.1"/>
        <property type="gene ID" value="ENSEASG00005001329.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106823177"/>
    <dbReference type="KEGG" id="eai:106823177"/>
    <dbReference type="CTD" id="8760"/>
    <dbReference type="GeneTree" id="ENSGT00940000158877"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004605">
        <property type="term" value="F:phosphatidate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016024">
        <property type="term" value="P:CDP-diacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000374">
        <property type="entry name" value="PC_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016720">
        <property type="entry name" value="PC_Trfase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13773:SF4">
        <property type="entry name" value="PHOSPHATIDATE CYTIDYLYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01148">
        <property type="entry name" value="CTP_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018269">
        <property type="entry name" value="PC_trans_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01315">
        <property type="entry name" value="CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 24" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="20 24" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="21 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="18 24 25" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="22 24" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="23 24" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16 24 25" id="KW-0808">Transferase</keyword>
    <keyword evidence="17 24 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="19 24" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="124"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="168"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="193"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="221"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00000859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00001056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00001617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00001729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00001902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00005119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00010185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00012487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRNR" id="PIRNR018269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU003938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005048511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="445" mass="51323" checksum="0DA52EF22BE1EA59" modified="2023-09-13" version="1">MTELRQRTAREPDGPPEDKESESEAKVDGETASDSESRAEASPPLIPADDTPEVLNRAFSSLSSRWKNWWVRGILTLAMIAFFFVIIYLGPLVLMIIVMCVQIKCFHEIITIGYNVYHSYDLPWFRTLSWYFLLCVNYFFYGETVTDYFFTLVQREEPLRILSKYHRFISFTLYLTGFCMFVLSLVKKHYRLQFYMFGWTHVTLLIVVTQSHLVIHNLFEGMIWFIVPISCVICNDIMAYMFGFFFGRTPLIKLSPKKTWEGFIGGFFATVVFGLLLSYVMSGYRCFVCPVEYNNDTNSFTVDCEPSDLFRLQEYVIPGVIQSIIGWKIVRMYPFQIHSIALSTFASLIGPFGGFFASGFKRAFKIKDFANTIPGHGGIMDRFDCQYLMATFVNVYIASFIRGPNPSKLIQQFLTLRPDQQLHIFNTLKSHLTDKGMLTAAAEDE</sequence>
</entry>
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            <fullName evidence="11">Kinesin-like protein</fullName>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
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                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Microtubule-based molecular motor that transport intracellular cargos, such as vesicles, organelles and protein complexes. Uses ATP hydrolysis to generate force to bind and move along the microtubule. Plays a role in cilia formation. Involved in photoreceptor integrity and opsin trafficking in rod photoreceptors. Transports vesicles containing N-methyl-D-aspartate (NMDA) receptor subunit GRIN2A into neuronal dendrites.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014712206.1">
        <property type="nucleotide sequence ID" value="XM_014856720.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J686"/>
    <dbReference type="Ensembl" id="ENSEAST00005058016.1">
        <property type="protein sequence ID" value="ENSEASP00005048679.1"/>
        <property type="gene ID" value="ENSEASG00005011585.2"/>
    </dbReference>
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    <dbReference type="KEGG" id="eai:106840981"/>
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    <dbReference type="GeneTree" id="ENSGT00940000153739"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
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        <property type="component" value="Chromosome 6"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="C:kinesin II complex"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="C:microtubule"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="C:neuron projection"/>
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        <property type="project" value="GOC"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:microtubule binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008088">
        <property type="term" value="P:axo-dendritic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="P:mitotic cell cycle"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0031503">
        <property type="term" value="P:protein-containing complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01371">
        <property type="entry name" value="KISc_KIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Kinesin_motor_dom_sf"/>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968">
        <property type="entry name" value="CENTROMERE PROTEIN E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47968:SF76">
        <property type="entry name" value="KINESIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="KINESIN_MOTOR_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6 12" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="4 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="14">
        <location>
            <begin position="10"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="251"/>
            <end position="284"/>
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        <location>
            <begin position="393"/>
            <end position="418"/>
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    </feature>
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        <location>
            <begin position="746"/>
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        </location>
    </feature>
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        <location>
            <begin position="449"/>
            <end position="617"/>
        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="261"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="394"/>
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        </location>
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    <feature type="binding site" evidence="10">
        <location>
            <begin position="97"/>
            <end position="104"/>
        </location>
        <ligand>
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        </ligand>
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    </evidence>
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    </evidence>
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    </evidence>
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</entry>
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    <accession>A0A9L0J6G3</accession>
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        <scope>IDENTIFICATION</scope>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JG04"/>
    <dbReference type="Ensembl" id="ENSEAST00005070045.1">
        <property type="protein sequence ID" value="ENSEASP00005048140.1"/>
        <property type="gene ID" value="ENSEASG00005012084.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155429"/>
    <dbReference type="OMA" id="DWNPSRE"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0072594">
        <property type="term" value="P:establishment of protein localization to organelle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051043">
        <property type="term" value="P:regulation of membrane protein ectodomain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="TM4SF9_like_LEL"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Tetraspanin"/>
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        <property type="entry name" value="Tetraspanin/Peripherin"/>
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    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
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    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF470">
        <property type="entry name" value="TETRASPANIN-17"/>
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        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TMFOUR"/>
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    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="20"/>
            <end position="44"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="64"/>
            <end position="86"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="285"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005048140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="345" mass="38005" checksum="ACA84C778C130C87" modified="2023-09-13" version="1">MPGKHQHFQEPEVGCCGKYFLFGFNIVFWVLGALFLAIGLWAWGEKGVLSNLSALTDLGGLDPVWLFVVVGGIMSVLGFAGCIGALRENTFLLKFFSVFLGLIFFLELATGILAFVFKDWIRDQLNLFINNNVKAYRDDIDLQNLIDFAQEYWSCCGARGPNDWNLNIYFNCTDLNPSRERCGVPFSCCVRDPAEDVLNTQCGYDVRLKLELEQQGSIHTKGCVGQFEKWLQDNLIVVAGVFVGIALLQVLPRPCLPHPALGPPAPLAASPLPALCPTDLRYLPGPEPRERHQGSEGQLVRLPRWPWPPARPTQGAPTVPVGLQEVFLLGPEPHMGLACGCAVGP</sequence>
</entry>
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    <name>A0A9L0JGE7_EQUAS</name>
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        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106833410</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the G-protein coupled receptor 1 family.</text>
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    <dbReference type="RefSeq" id="XP_014700088.1">
        <property type="nucleotide sequence ID" value="XM_014844602.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A9L0JGE7"/>
    <dbReference type="Ensembl" id="ENSEAST00005083621.1">
        <property type="protein sequence ID" value="ENSEASP00005051418.1"/>
        <property type="gene ID" value="ENSEASG00005028038.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106833410"/>
    <dbReference type="KEGG" id="eai:106833410"/>
    <dbReference type="GeneTree" id="ENSGT00940000163199"/>
    <dbReference type="OrthoDB" id="5969463at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15226">
        <property type="entry name" value="7tmA_OR4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000012">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050427">
        <property type="entry name" value="Olfactory_Receptors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48002">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005051418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="312" mass="35146" checksum="E4102EA395080D82" modified="2023-09-13" version="1">MDEANRSVVSEFVFVGFSNSWEIQLLIFLFSSVFYVASLMGNLLIVLTVTFNLHLQSPMYFLLANLSIMDLIFCSSTAPKMIYDLLRKHKTISFGGCVVQIFFIHTVGGAEMVLLVTMAFDRYIAICKPLHYLTIISPGRCLLFLIASWIIGLIHSMTQLAFVVDLPFCGPNELDSFFCDLPQFIKLACIQTFTLEFMVTANSGLLSVATFLILIISYIFILVTVQKKSSGGISKALSTLSAHVTVVVLFFGPLIFFYVWPFPTSHLDKFLAIFDSVITPFLNPVIYTFRNKEIQVAMRRLCTKVVNYSKMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JJB4</accession>
    <name>A0A9L0JJB4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transmembrane protein 177</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC123285330</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in the early steps of cytochrome c oxidase subunit II (MT-CO2/COX2) maturation and is required for the stabilization of COX20 and the newly synthesized MT-CO2/COX2 protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM177 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JJB4"/>
    <dbReference type="Ensembl" id="ENSEAST00005082775.1">
        <property type="protein sequence ID" value="ENSEASP00005053431.1"/>
        <property type="gene ID" value="ENSEASG00005029059.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010354"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026620">
        <property type="entry name" value="TMEM177"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21824">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21824:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 177"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005053431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="314" mass="34006" checksum="370C89B9883ADDD2" modified="2023-09-13" version="1">MAGPLWRASAFVQRHRTGLLVGSCAGLFGAQISYHLFPDPVVQWLYQYWPQGQPAPLSPELQRLFQEVLQDMGVPSGHCYEAFTTFTTFTFQPVSAGFPRLPAGAVVGIPASFLGGPVTHSDQPVIIHGHRVNWQSPSGARLRDALTLSRDAQKFALAREVVYLESAAAALQALPAPACLAGTWALGVGAKHALGLYGGPMNLRAAFNLVAAVAGFVAYAFSTDSLTHALEAWLDRRTASLSAAYARGGVEFYEKVLSGNLALRGLLGRPGEKLYTPSGNVAPRHWFRIKHLPYTTRRDSVLQLWRATLTPGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JJD1</accession>
    <name>A0A9L0JJD1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="27">Non-POU domain-containing octamer-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="32" type="primary">NONO</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="26">Monomer and component of the SFPQ-NONO complex, which is probably a heterotetramer of two 52 kDa (NONO) and two 100 kDa (SFPQ) subunits. NONO is a component of spliceosome and U5.4/6 snRNP complexes. Interacts with CPNE4 (via VWFA domain). Forms heterodimers with PSPC1; this involves formation of a coiled coil domain by helices from both proteins. Part of complex consisting of SFPQ, NONO and MATR3. Part of a complex consisting of SFPQ, NONO and NR5A1. Part of a complex consisting of SFPQ, NONO and TOP1. Interacts with SPI1 and SPIB. Interacts with RNF43. Interacts with PER1 and PER2. Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 RNA. Interacts (via second RRM domain) with WASL; the interaction is direct. Component of a multiprotein complex with WASL and SFPQ. Interacts with ERCC6. Interacts (via DNA-binding domain) with TET1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044620244.1">
        <property type="nucleotide sequence ID" value="XM_044764309.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044620245.1">
        <property type="nucleotide sequence ID" value="XM_044764310.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JJD1"/>
    <dbReference type="SMR" id="A0A9L0JJD1"/>
    <dbReference type="Ensembl" id="ENSEAST00005054611.1">
        <property type="protein sequence ID" value="ENSEASP00005052463.1"/>
        <property type="gene ID" value="ENSEASG00005031467.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106828875"/>
    <dbReference type="KEGG" id="eai:106828875"/>
    <dbReference type="CTD" id="4841"/>
    <dbReference type="GeneTree" id="ENSGT00940000154442"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042382">
        <property type="term" value="C:paraspeckles"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903377">
        <property type="term" value="P:negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042752">
        <property type="term" value="P:regulation of circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12946">
        <property type="entry name" value="NOPS_p54nrb_PSF_PSPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12588">
        <property type="entry name" value="RRM1_p54nrb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000226">
        <property type="entry name" value="Non-POU domain-containing octamer-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000043">
        <property type="entry name" value="paraspeckle component 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012975">
        <property type="entry name" value="NOPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034552">
        <property type="entry name" value="p54nrb_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23189">
        <property type="entry name" value="RNA RECOGNITION MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08075">
        <property type="entry name" value="NOPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16" id="KW-0007">Acetylation</keyword>
    <keyword evidence="20" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="18 29" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="22" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="24" id="KW-0234">DNA repair</keyword>
    <keyword evidence="19" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="14" id="KW-0391">Immunity</keyword>
    <keyword evidence="9" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="23" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="25" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="15 28" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="21" id="KW-0804">Transcription</keyword>
    <keyword evidence="17" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="RRM" evidence="31">
        <location>
            <begin position="73"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="31">
        <location>
            <begin position="147"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="442"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="29">
        <location>
            <begin position="274"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="30">
        <location>
            <begin position="14"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00063155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00068166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="470" mass="54103" checksum="8066171D9FEA98DD" modified="2023-09-13" version="1">MQSNKTFNLEKQNHTPRKHHQHHHQQHHQQQQQQPPPPPIPANGQQASSQNEGLTIDLKNFRKPGEKTFTQRSRLFVGNLPPDITEEEMRKLFEKYGKAGEVFIHKDKGFGFIRLETRTLAEIAKVELDNMPLRGKQLRVRFACHSASLTVRNLPQYVSNELLEEAFSVFGQVERAVVIVDDRGRPSGKGIVEFSGKPAARKALDRCSEGSFLLTTFPRPVTVEPMDQLDDEEGLPEKLVIKNQQFHKEREQPPRFAQPGSFEYEYAMRWKALIEMEKQQQDQVDRNIKEAREKLEMEMEAARHEHQVMLMRQDLMRRQEELRRMEELHNQEVQKRKQLELRQEEERRRREEEMRRQQEEMMRRQQEGFKGTFPDAREQEIRMGQMAMGGAMGINNRGAMPPAPVPAGTPAPPGPATMMPDGTLGLTPPTTERFGQAATMEGIGAIGGTPPAFNRAAPGAEFAPNKRRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JK48</accession>
    <name>A0A9L0JK48_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Beta-alanine-activating enzyme</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Acyl-CoA synthetase family member 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">AASDH</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATP-dependent AMP-binding enzyme family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JK48"/>
    <dbReference type="Ensembl" id="ENSEAST00005037737.1">
        <property type="protein sequence ID" value="ENSEASP00005049555.1"/>
        <property type="gene ID" value="ENSEASG00005017782.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000033811"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043041">
        <property type="term" value="P:amino acid activation for nonribosomal peptide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019482">
        <property type="term" value="P:beta-alanine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17654">
        <property type="entry name" value="A_NRPS_acs4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12780:FF:000027">
        <property type="entry name" value="AASDH isoform 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.30:FF:000014">
        <property type="entry name" value="acyl-CoA synthetase family member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001301">
        <property type="entry name" value="Beta-alanine-activating enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048005">
        <property type="entry name" value="AASDH_AMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052091">
        <property type="entry name" value="Beta-ala_Activ/Resist"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018391">
        <property type="entry name" value="PQQ_b-propeller_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002372">
        <property type="entry name" value="PQQ_rpt_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44394">
        <property type="entry name" value="BETA-ALANINE-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44394:SF1">
        <property type="entry name" value="BETA-ALANINE-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13570">
        <property type="entry name" value="Beta-prop_ACSF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00564">
        <property type="entry name" value="PQQ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="12">
        <location>
            <begin position="554"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00078906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005049555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1099" mass="122663" checksum="D6B16AC690434EBF" modified="2023-09-13" version="1">MTLQELVHQAASLYSDKIAVCFDECNNQPPVYYTYKTVINAASELSNFLLLHCDFQGIRAIGLYCHPGINLPSWILGILQVPAAYAPIDPDSPPALSIHFMKKCNLKFILVEKQQINKFKSSYETLLNYDTFTVEHNDIVLFRLHWKNVEVSLMLNERKEKYEKEKMTNSTSSENSNEEKSEEHMDAKLKHCLAYVLHTSGTTGKPKIVRVPHACIVPNIQHFRVLFEITQDDVLFLASPLTFDPSVVEIFVALSSGASLLIVPTSVKVIPSKLAAVLFSHHRVTVLQATPTLLRRFGSQLIKSTVLSTSTSLRVLALGGEAFPSLTVLKSWRGVGNKTQIFNIYGITEVSSWATFYRIPEKTLNSTLKCELPVQLGFPLLGTVVEVRDTDGFAIQEGDGQVFLGGRNRVCFLDDEVTVPLGTMRATGDFVTVKDGEIFFLGRKDSQIKRHGKRLNIELVQQVAEGLQEVESCAVTWYNQEKLILFMVSKNDLVKDYIFEELQKHLPSHAIPDELVLIDSLPFTSHGKIDVSELNKIYLNYINLKSECKLNGKEELWEKLQHLWKSILSLSEDLLRIPDESLFLNSGGDSLKSIRLLNEIEKLVGTSVPGLLEIILNSSILEIYNHIVQTVFPDEDLTFSKNYATKRKFSDINQETSGKSLHQNSVMPLNCDKKINAFIALSRGGQILSVNTTRPLTKLGHCPSACSSDLISQTNTQNVESLNPPALSGKSKDPSCVAEFFSEEGTPVIEAEKMELCVRWMSDTGKCVDASPLVVIPAVDKSSATVYIGSHSHRMMAVDLYSGKVKWEQILGDRVESSACVSKCGNFIVVGCYNGLVYVLKSSSGEKYWTFTTEDAVKSSATMDPTTGLLYIGSHDQHAYALDIYKKKCVWKLKCGGTVFSSPCLSLTPHHLYFATLGGLLLAVNPATGNRIWKHSCGKPLFSSPRCCLQYICIGCVDGNLLCFTHFGEQVWQFCTSEPIFSSPCTSASEQEIFFGSHDCFIYCCNTKGHLQWKFETTSRVYSTPFNFHNYNRSDEMLLAAASTDGKLWILESKSGQLQSVYRLPGEVFSSPVVWESMLIIGCRNNYVYCLDLLGGNQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JLK3</accession>
    <name>A0A9L0JLK3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
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                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JLK3"/>
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        <property type="protein sequence ID" value="ENSEASP00005054099.1"/>
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        <property type="component" value="Chromosome 22"/>
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        <location>
            <begin position="159"/>
            <end position="281"/>
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            <begin position="173"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="281" mass="29028" checksum="D9960546BD297E11" modified="2023-09-13" version="1">MVLNLTQISPAAKEPALLQVTNPVPPHVPPVTSPHPGADLTAVDPVRGKTALEWALLTDSFDTVCRIRQLLRRPQVEQLSQYYQPEWPALPGLVAQAQAQAQAAPSLLERLQTTLSLPFAQSPQEGGVLDHLVTITTSLASPFLTTACQTLCPDHPPALGTRSKSVPELLGSAPPPPPVPPAPSGGPRLPGLRPLPEPSGHVEHVPSVATAQGQYQPQAPSSQDPPLQGTLISLAVQAEAPAVRASNAGPSSLEIPGAQDGEEEAGGGGGGQVVTEPGEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JPJ7</accession>
    <name>A0A9L0JPJ7_EQUAS</name>
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        <recommendedName>
            <fullName evidence="11">Tubby-related protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Tubby superfamily protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Tubby-like protein 4</fullName>
        </alternativeName>
    </protein>
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        <name evidence="17" type="primary">TULP4</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
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                <person name="Liu"/>
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                <person name="Wang"/>
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                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
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                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="J."/>
                <person name="Li"/>
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                <person name="Liu"/>
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                <person name="G."/>
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                <person name="J."/>
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                <person name="S."/>
                <person name="Jordana"/>
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                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TUB family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005045605.1">
        <property type="protein sequence ID" value="ENSEASP00005054338.1"/>
        <property type="gene ID" value="ENSEASG00005018912.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155913"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000262">
        <property type="entry name" value="Tubby like protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.90.10:FF:000002">
        <property type="entry name" value="Tubby like protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.90.10">
        <property type="entry name" value="Tubby Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056159">
        <property type="entry name" value="Beta-prop_IFT121_TULP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001496">
        <property type="entry name" value="SOCS_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025659">
        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF2">
        <property type="entry name" value="TUBBY-RELATED PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24797">
        <property type="entry name" value="Beta-prop_WDR35_TULP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07525">
        <property type="entry name" value="SOCS_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54518">
        <property type="entry name" value="Tubby C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50225">
        <property type="entry name" value="SOCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="7 14" id="KW-0853">WD repeat</keyword>
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            <begin position="78"/>
            <end position="109"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="1005"/>
            <end position="1021"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="1057"/>
            <end position="1073"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1328"/>
            <end position="1346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056629"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00071888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50225"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005054338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1542" mass="168453" checksum="744D3F23B1D1896D" modified="2023-09-13" version="1">MYAAVEHGPVLCSDSNILCLSWKGRVPKSEKEKPVCRRRSYEEGWLATGNGRGVVGVTFTSSHCRRDRNTPQRVNFNLRGHNSEVVLVRWNEPYQKLATCDADGGIFVWIQYEGRWSVELVNDRGAQVSDFTWSHDGTQALISYRDGFVLVGSVSGQRHWSSEINLESQITCGIWTPDDQQVLFGTADGQVIVMDCHGRMLAHVLLHESDGILSMSWNYPAFLVEDSSESDTDSDDYSPPQDGPAAYPIPVQNIKPLLTVSFTSGDISLMNSYDDLSPTVIRSGLKEVVAQWCTQGDLLAVAGMERQTQLGDLPSGPLLKSAMVKFYNVRGEHIFTLDTLVQRPIISICWGHRDSRLLMASGPALYVVRVEHRVSSLQLLCQQAIASALREDKDVSKLTLPPRLCSYLSTAFIPTIKPPIPDPNNMRDFVSYPSAGNERLHCTMKRTEDDPEVGGPCYTLYLEYLGGLVPILKGRRVSKLRPEFVIMDPRTDSKADEIYGNSLISTMIDSCNCSDSSDIELSDDWAAKKSPKISRASKSPKLPRINIEARKSPKLSRAAQEISRSPRLPMRKPSIGSPSLTRREFPFEDITQHNYLAQVTSNIWGTKFKIVGLAAFLPTNLGAVIYKTSLLHLQPRQMTIYLPEVRKISVDYVNLPVFNPNVFSEDEDDLPVTGASGVPENNPPCTVNIPIAPIHSSAQAMSPTQSIGLVQSLLANQNVQLDVLTNQTTAVGAAEHASDSATQYPISNRYSNPGQVIFGGVEMGRIIPNPPQLSLPPPAQGAIQLSVVDHGDRDHEHLQKPAKALRPVPQLAAEGDAVVFSAPQEVQVAKMNPPPPYPGTIPAAPTTAAPPPPLPPPQPPVDVCLKKGDFSLYPTAAHYQTPLGYERITTFDSSGNVEEVCRPRTRMLCSQNTYTLPGPGSSATLRLTATEKKVPQPCTSATLNRLTVPRYSIPPGDPPPYPDLAGPLGAGRAAQRPESSLIHATLRRNNREAALRAAQLAESPRAPPQLPAKPKGAPRPSPRARRPPYTCSQCSGAGAAGRPEQSTGAGPGAHAASTSPLSSQSSYSLLSPPDGARERADYVNSAFTEDEALGQHCPAEKPPRHPALPEAAAAGKRPPPYQWDPMLGEDIWVPQERTAQTAVPNPLKLSPLMVAQSQHLDVSRVPFVSPKSPASPTATFQTGYGMGVPYPGSYNTPPLPGVQAPCSPKDALSPAQFAQQESAVVLQPAYPPSLSYCALPPMYPGSSTCSSLQPPPIALHPWNSYSACPPVQNPQGTLPTKAHLVVEKPLVSPPPTDLQSHLGTEVMVETADNFQEVLSLTESPVPQRTEKFGKKNRKRLDSRAEEGNVQAITEGKMKKEARTLSDFNSLISSPRLGREKKKVKSQKDQLKSKKLNKTNEFQDSSESEPELFISGDELMNQSQGSRKGWKSKRSLRTASELEEAKCRKASEREDGRLGSQGFVYVMANKQPLWNEATQVYQLDFGGRVTQESAKNFQIELEGRQVMQFGRIDGNAYILDFQYPFSAVQAFAVALANVTQRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JS00</accession>
    <name>A0A9L0JS00_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Importin-11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Ran-binding protein 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">IPO11</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Interacts with UBE2E3 and RPL12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the importin beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014704009.1">
        <property type="nucleotide sequence ID" value="XM_014848523.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JS00"/>
    <dbReference type="Ensembl" id="ENSEAST00005057710.1">
        <property type="protein sequence ID" value="ENSEASP00005056086.1"/>
        <property type="gene ID" value="ENSEASG00005006951.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835918"/>
    <dbReference type="KEGG" id="eai:106835918"/>
    <dbReference type="CTD" id="51194"/>
    <dbReference type="GeneTree" id="ENSGT00390000014071"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000116">
        <property type="entry name" value="importin-11 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058669">
        <property type="entry name" value="TPR_IPO7/11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF7">
        <property type="entry name" value="IMPORTIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25758">
        <property type="entry name" value="TPR_IPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="14">
        <location>
            <begin position="28"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00062902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00077811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005056086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="975" mass="112489" checksum="F14DFB623B70E285" modified="2023-09-13" version="1">MDLNSASTVVLQVLTQATSQDTAVLKPAEEQLKQWETQPGFYSVLLNIFTNHTLDINVRWLAVLYFKNGIDRYWRRVAPHALSEEEKSTLRAGLITNFNEPINQIATQIAVLIAKVARLDCPRQWPELIPTLIESVKVQDDLRQHRALLTFYHVTKTLASKRLAADRKLFYDLASGIYNFACSLWNHHTDTFLQQVSSGNEAAVLSSLERTLLSLKVLRKLTVNGFVEPHKNMEVMGFLHGIFDRLKQFLECSRSVGTNNVCRDRLEKTIILFTKVLLDFLDQHPFSFTPLIQRSLEFSVSYVFTEVGEGITFERFIVQCMNLIKMIVKNYAYKPSKNFEDSSPETLEAHKIKMAFFTYPTLTEICRRLVSHYFLLTEEELTMWEEDPEGFTVEETGGDSWKYSLRPCTEVLFIDIFHEYNQTLTPVLLEMMQTLQGPTNVEDMNALLIKDAVYNAVGLAAYELFDSVDFDQWFKNQLLPELQVIHNRYKPLRRRVIWLIGQWISVKFKSDLRPMLYEAICNLLQDQDLVVRIETATTLKLTVDDFEFRTDQFLPYLETMFTLLFQLLQQVTECDTKMHVLHVLSCVIERVNMQIRPYVGCLVQYLPLLWKQSEEHNMLRCAILTTLIHLVQGLGADSKNLYPFLLPVIQLSTDVSQPPHVYLLEDGLELWLVTLENSPCITPELLRIFQNMSPLLERSSENLRTCFKIINGYIFLSSTEFLQTYAVGLCQSFCELLKEITTEGQVQVLKVVENALKVNPVLGPQMFQPILPYVFRGIIEGERYPVVMSTYLGVMGRVLLQNTSFFSSLLNEMAHKFNQEMDQLLGNMIEMWVDRMDNITQPERRKLSALALLSLLPSDNSVIQDKFCGIINISVEGLHDVMTEDPETGTYKDCMLMSHLEEPKVTEDEEPPTEQDKRKKILALKDPVHTVSLQQFIYEKLKAQQELLGEQGFQSLMETVDTEIVTQLQEFLQGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JSC1</accession>
    <name>A0A9L0JSC1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitogen-activated protein kinase kinase kinase 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">MAPK/ERK kinase kinase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">MAP3K1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. May phosphorylate the MAPK8/JNK1 kinase. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005054352.1">
        <property type="protein sequence ID" value="ENSEASP00005052897.1"/>
        <property type="gene ID" value="ENSEASG00005011958.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159154"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd16494">
        <property type="entry name" value="RING-CH-C4HC3_ZSWM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06630">
        <property type="entry name" value="STKc_MEKK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000286">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 1 (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000122">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 1 (Predicted)"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 1 (Predicted)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050538">
        <property type="entry name" value="MAP_kinase_kinase_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48016">
        <property type="entry name" value="MAP KINASE KINASE KINASE SSK2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21040">
        <property type="entry name" value="CEP104-like_TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="22">
        <location>
            <begin position="178"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="21">
        <location>
            <begin position="283"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="1079"/>
            <end position="1344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="44"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="351"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="443"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="726"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="778"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="64"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="256"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="726"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="801"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="817"/>
            <end position="831"/>
        </location>
    </feature>
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        <location>
            <begin position="853"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="881"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="902"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="1108"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00083881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1348" mass="148893" checksum="93F69C2734748081" modified="2023-09-13" version="1">RCEMENKETLKGLHKMEDRPEERMIREKLKATCMPAWKHEWLERRNRRGPVVVKPIPIKGDGSEINNLATESQGEGQASTASPAPKGRRSPSPGSSPSGRTVKSESPGVRRKRVSPVPFQSGRITPPRRAPSPDGFSPYSPEETSRRVNKVMRARLYLLQQIGPNSFLIGGDSPDNKYRVFIGPQNCSCGRGTFCIHLLFVMLRVFQLEPSDPMLWRKTLKNFEVESLFQKYHSRRSSRIKAPSRNTIQKFVSRMSNSHTLSSSSTSTSSSENSIKDEEEQMCPICLLGMLDEESLTVCEDGCRNKLHHHCMSIWAEECRRNREPLICPLCRSKWRSHDFYSHELSSPVDSPSSLRAAQQQTIQQQPLAGSQRRNQESNFNLTHYGTQQIPPAYKDLAEPWIQVFGMELVGCLFSRNWNVREMALRRLSHDVSGALLLANGESTGNSGSNGGSSPNAGAATGSSQTSISGDVVEACCNVLSMVCADPVYKVYVAALKTLRAMLVYTPCHSLAERIKLQRLLRPVVDTILVKCADANSRTSQLSISTLLELCKGQAGELAVGREILKAGSIGIGGVDYVLNCILGNQTESSNWQELLGRLCLIDRLLLEFPAEFYPHIVSTDVSQAEPVEVRYKKLLSLLTFALQSIDNSHSMVGKLSRRIYLSSARMVTAVPHVFSKLLEMLNVSSSTHFTRMRRRLMAIADEVEIAEAIQLGIEDTLDGRHDSFLQASVPNSYPETTENSSLERTDHLEKTGKGLCATKLSASSEDISDRLASISVGLPSSTTMEQPKPMVQTKGRPHSQCLNSSPLSHHSQVMFPAFSTPSSSAPSVPAGTVTDVSKQRPQGFVPCKIPSASPQTQRKFSLQFQRNCSENKDSEKLSPIFTQSRPLPSSNIHRPKPSRPTPGNTSKQGDASKNSMTLDLNSTSKCGDSFGNSSNSSNAVIPSDETVFTPVEEKCRLDVNTELNSSIEDLLEASMPSSDTTVTFKSEVAVLSPEKDESDDTYKDDVNHNQKCKEKMEAEEEEALAIAMAMSASQDALPIVPQLQVENGEDIIIIQQDTPETLPGHTKAKQPYREDTEWLKGQQIGLGAFSSCYQAQDVGTGTLMAVKQVTYVRNTSSEQEEVVEALREEIRMMSHLNHPNIIRMLGATCEKSNYNLFIEWMAGGSVAHLLSKYGAFKESVVINYTEQLLRGLSYLHENQIIHRDVKGANLLIDSTGQRLRIADFGAAARLASKGTGAGEFQGQLLGTIAFMAPEVLRGQQYGRSCDVWSVGCAIIEMACAKPPWNAEKHSNHLALIFKIASATTAPSIPSHLSPGLRDVALRCLELQPQDRPPSRELLKHPVFRTTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JSR1</accession>
    <name>A0A9L0JSR1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Vesicle associated membrane protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">VAMP2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044600219.1">
        <property type="nucleotide sequence ID" value="XM_044744284.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JSR1"/>
    <dbReference type="Ensembl" id="ENSEAST00005080497.1">
        <property type="protein sequence ID" value="ENSEASP00005052190.1"/>
        <property type="gene ID" value="ENSEASG00005038676.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106844236"/>
    <dbReference type="KEGG" id="eai:106844236"/>
    <dbReference type="CTD" id="6844"/>
    <dbReference type="GeneTree" id="ENSGT00940000158370"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15870">
        <property type="entry name" value="R-SNARE_VAMP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000013">
        <property type="entry name" value="Vesicle-associated membrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001388">
        <property type="entry name" value="Synaptobrevin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016444">
        <property type="entry name" value="Synaptobrevin/VAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45701">
        <property type="entry name" value="SYNAPTOBREVIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005409">
        <property type="entry name" value="Synaptobrevin_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00219">
        <property type="entry name" value="SYNAPTOBREVN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00417">
        <property type="entry name" value="SYNAPTOBREVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0770">Synapse</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="31"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="116" mass="12679" checksum="C8C679C4E7E0A803" modified="2023-09-13" version="1">MSATAATAPPAAPTGEGGPPAPPPNLTSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMIILGVICAIILIIIIVYFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JWT7</accession>
    <name>A0A9L0JWT7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JWT7"/>
    <dbReference type="Ensembl" id="ENSEAST00005074063.1">
        <property type="protein sequence ID" value="ENSEASP00005054507.1"/>
        <property type="gene ID" value="ENSEASG00005028730.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161272"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000366">
        <property type="entry name" value="GTPase, IMAP family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF74">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="4">
        <location>
            <begin position="24"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005054507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="288" mass="31191" checksum="8F9D464DBA1E754E" modified="2023-09-13" version="1">EGGKKRACGSRIIVRAGSEDNPQEPRLRLILAGRTGAGKSATGNSILGHRRFLSRLSAAQVTTTCAVGSCRWAGWHLDVIDTPDLFGAEDPRTEPGCGERGRCYLLSASGPHALLLVSQLGRFTAQDQQAARRLMAMFGDGAVARTVLLFTHKEEDLAGGTSLQDYVRCTDNRALREAGGRREAQVAELMALLERLVRAHGGAPYTNDVYGLARALGRARPEERLRRVAESVAARVQRRRGRGLLAALLGRGKAPWSRVRLVVAALLGALEIFLLFQCQNKGKQVFVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JX15</accession>
    <name>A0A9L0JX15_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">RAR related orphan receptor C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">RORC</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070353027.1">
        <property type="nucleotide sequence ID" value="XM_070496926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JX15"/>
    <dbReference type="Ensembl" id="ENSEAST00005054163.1">
        <property type="protein sequence ID" value="ENSEASP00005054582.1"/>
        <property type="gene ID" value="ENSEASG00005005204.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106848355"/>
    <dbReference type="CTD" id="6097"/>
    <dbReference type="GeneTree" id="ENSGT00940000161521"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06968">
        <property type="entry name" value="NR_DBD_ROR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06939">
        <property type="entry name" value="NR_LBD_ROR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.565.10:FF:000005">
        <property type="entry name" value="Nuclear orphan receptor ROR-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.50.10:FF:000003">
        <property type="entry name" value="Nuclear orphan receptor ROR-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.565.10">
        <property type="entry name" value="Retinoid X Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035500">
        <property type="entry name" value="NHR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044101">
        <property type="entry name" value="NR_DBD_ROR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000536">
        <property type="entry name" value="Nucl_hrmn_rcpt_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001723">
        <property type="entry name" value="Nuclear_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003079">
        <property type="entry name" value="ROR_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001628">
        <property type="entry name" value="Znf_hrmn_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45805">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR HR3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45805:SF1">
        <property type="entry name" value="NUCLEAR RECEPTOR ROR-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00105">
        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01293">
        <property type="entry name" value="RORNUCRECPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00398">
        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00047">
        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48508">
        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51843">
        <property type="entry name" value="NR_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00031">
        <property type="entry name" value="NUCLEAR_REC_DBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51030">
        <property type="entry name" value="NUCLEAR_REC_DBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Nuclear receptor" evidence="14">
        <location>
            <begin position="7"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="NR LBD" evidence="15">
        <location>
            <begin position="249"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="83"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005054582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="498" mass="56003" checksum="7573104FDCB121C1" modified="2023-09-13" version="1">MRTQIEVIPCKICGDKSSGIHYGVITCEGCKGFFRRSQQCNVAYSCTRQQNCPIDRTSRNRCQHCRLQKCLALGMSRDAVKFGRMSKKQRDSLHAEVQKQLQQQQQQQQREQAAKTPPAGGQGADPLPCTLGLPDGQLPLGSSPDLPEASACPPGLLRAPGSGPSYSNSLAKAGLNGASYHLEYSPERGKAEGRESFYSTGSQLTPNRCGLHFEEPRHPGLGEPRQGLDSYCSPGFRSTTEAPYASLTETEHLVQNVCKSYRETCQLRLEDLLRQRSNIFSREEVASYQRKSMWEMWERCAHRLTEAIQYVVEFAKRLSGFMELCQNDQIVLLKAGAMEVVLVRMCRAYNADNHTVFFEGKYGGMELFRALGCSELISSIFDFSRSLSALRFSEDEIALYTALVLINANRPGLQEKRKVEQLQYNLEMAFHHHLCKTHRQGILAKLPPKGKLRSLCSQHVEKLQTFQHLHPIVVQAAFPPLYKELFSTEMESPEGLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JYA2</accession>
    <name>A0A9L0JYA2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein kinase C-binding protein NELL2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">NEL-like protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">NELL2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014691774.1">
        <property type="nucleotide sequence ID" value="XM_014836288.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014691775.1">
        <property type="nucleotide sequence ID" value="XM_014836289.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070350824.1">
        <property type="nucleotide sequence ID" value="XM_070494723.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070350825.1">
        <property type="nucleotide sequence ID" value="XM_070494724.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JYA2"/>
    <dbReference type="Ensembl" id="ENSEAST00005072682.1">
        <property type="protein sequence ID" value="ENSEASP00005058266.1"/>
        <property type="gene ID" value="ENSEASG00005023499.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106828051"/>
    <dbReference type="KEGG" id="eai:106828051"/>
    <dbReference type="CTD" id="4753"/>
    <dbReference type="GeneTree" id="ENSGT00810000125439"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005080">
        <property type="term" value="F:protein kinase C binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000121">
        <property type="entry name" value="Neural EGFL like 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000120">
        <property type="entry name" value="Protein kinase C-binding protein NELL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000211">
        <property type="entry name" value="Protein kinase C-binding protein NELL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000015">
        <property type="entry name" value="protein kinase C-binding protein NELL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000102">
        <property type="entry name" value="Protein kinase C-binding protein NELL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000111">
        <property type="entry name" value="Protein kinase C-binding protein NELL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000023">
        <property type="entry name" value="protein kinase C-binding protein NELL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.200.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024731">
        <property type="entry name" value="NELL2-like_EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051586">
        <property type="entry name" value="PKC-binding_NELL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048287">
        <property type="entry name" value="TSPN-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001007">
        <property type="entry name" value="VWF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24042">
        <property type="entry name" value="NEL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24042:SF0">
        <property type="entry name" value="PROTEIN KINASE C-BINDING PROTEIN NELL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12947">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00093">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00210">
        <property type="entry name" value="TSPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00214">
        <property type="entry name" value="VWC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00215">
        <property type="entry name" value="VWC_out"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57603">
        <property type="entry name" value="FnI-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01208">
        <property type="entry name" value="VWFC_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50184">
        <property type="entry name" value="VWFC_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0106">Calcium</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 9" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040339410" description="Protein kinase C-binding protein NELL2" evidence="11">
        <location>
            <begin position="22"/>
            <end position="816"/>
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    </feature>
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        <property type="entry name" value="TRANSCRIPTION FACTOR ONECUT"/>
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        <property type="entry name" value="HOMEOBOX_2"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 10" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="328"/>
            <end position="414"/>
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        <location>
            <begin position="428"/>
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            <end position="46"/>
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            <begin position="494"/>
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    <sequence length="508" mass="55485" checksum="9BA32FEEE233023E" modified="2023-09-13" version="1">MKAAYTAYRCLTKDLEGCAMNPELTMESLGTLHGPAGRRQRRRGRRGGGGGGAAAGGRAMSRSCWPVPAPTTRAVAPLARCGAPPPPPPTAHQELGTAAAAAAAASRSAMVTSMASILDGGDYRPELSIPLHHAMSMSCDSSPPGMGMSNTYTTLTPLQPLPPISTVSDKFHHPHPHHHPHHHHHHHHQRLSGNVSGSFTLMRDERGLPAMNNLYSPYKEMPGMSQSLSPLAATPLGNGLGGLHNAQQSLPNYGPPGHDKMLSPNFDAHHTAMLTRGEQHLSRGLGTPPAAMMSHLNGLHHPSHAQSHGPVLAPSRERPPSSSSGSQVATSGQLEEINTKEVAQRITAELKRYSIPQAIFAQRVLCRSQGTLSDLLRNPKPWSKLKSGRETFRRMWKWLQEPEFQRMSALRLAACKRKEQEPNKDRNNSQKKSRLVFTDLQRRTLFAIFKENKRPSKEMQITISQQLGLELTTVSNFFMNARRRSLEKWQDDLSTGGSSSTSSTCTKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K3K3</accession>
    <name>A0A9L0K3K3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Protein phosphatase 1 regulatory subunit 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">RNA polymerase II subunit 5-mediating protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">URI1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Plays a central role in maintaining S6K1 signaling and BAD phosphorylation under normal growth conditions thereby protecting cells from potential deleterious effects of sustained S6K1 signaling. The URI1-PPP1CC complex acts as a central component of a negative feedback mechanism that counteracts excessive S6K1 survival signaling to BAD in response to growth factors. Mediates inhibition of PPP1CC phosphatase activity in mitochondria. Coordinates the regulation of nutrient-sensitive gene expression availability in a mTOR-dependent manner. Seems to be a scaffolding protein able to assemble a prefoldin-like complex that contains PFDs and proteins with roles in transcription and ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homodimer. Component of the PAQosome complex which is responsible for the biogenesis of several protein complexes and which consists of R2TP complex members RUVBL1, RUVBL2, RPAP3 and PIH1D1, URI complex members PFDN2, PFDN6, PDRG1, UXT and URI1 as well as ASDURF, POLR2E and DNAAF10/WDR92. Interacts with POLR2E/RPB5, RUVBL2 and RUVBL1. Interacts with PFDN2, PFDN4 and STAP1; the interactions are phosphorylation-dependent and occur in a growth-dependent manner in the mitochondrion. Interacts with UXT. Interacts with PPP1CC; the interaction is phosphorylation-dependent and occurs in a growth factor-dependent manner. Interacts (via the middle C-terminal region) with GTF2F1 and GTF2F2. Interacts with DMAP1. Interacts with TSC1 and TSC2. Interacts with PRPF8 and EFTUD2 in a ZNHIT2-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the RNA polymerase II subunit 5-mediating protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K3K3"/>
    <dbReference type="Ensembl" id="ENSEAST00005082760.1">
        <property type="protein sequence ID" value="ENSEASP00005060061.1"/>
        <property type="gene ID" value="ENSEASG00005020967.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002362"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990062">
        <property type="term" value="C:RPAP3/R2TP/prefoldin-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051219">
        <property type="term" value="F:phosphoprotein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004864">
        <property type="term" value="F:protein phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071363">
        <property type="term" value="P:cellular response to growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071383">
        <property type="term" value="P:cellular response to steroid hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001243">
        <property type="term" value="P:negative regulation of intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001558">
        <property type="term" value="P:regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009615">
        <property type="term" value="P:response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd23159">
        <property type="entry name" value="Prefoldin_URI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000008">
        <property type="entry name" value="unconventional prefoldin RPB5 interactor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052255">
        <property type="entry name" value="RNA_pol_II_subunit5-mediator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15111">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT 5-MEDIATING PROTEIN NNX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15111:SF0">
        <property type="entry name" value="UNCONVENTIONAL PREFOLDIN RPB5 INTERACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0650">Protein phosphatase inhibitor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="205"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="273"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="330"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="432"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="497"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="19">
        <location>
            <begin position="103"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="20">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="284"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023272"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023273"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00038295"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00053952"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00064379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00078910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005060061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="520" mass="58617" checksum="19F2AC8BA2655FC1" modified="2023-09-13" version="1">MEAPRSAPGPPRRPPPRRCAPRRWRGSARSRKRKKVDNDYNALQERLSTLPDKLSYNIMVPFGPFAFMPGKLVHTNEVTVLLGDNWFAKCSAKQAVGLVEHRKEHVRKTIDDLKKVMKNFESRVEFTEDLQKMSDAAGDIVDIREEIRSDFEFKAKHRIAHKPHSKPKTSDIFEADFANDVKSKDLLADKELWARLEELERQEELLGELDSKPDTLIANGEDTTSSEEEKEDQNINVNVMHQVTDSVTPSNCCEDVTNSELFNGQTNSQLNCSVNGANSYHSNEEEDDDDDDDEDDDGDGDNENDHDTLGVGDNSIPTIYFSHTVEPKRVRINTGKNTTLKFSEKKEEAKRKRKNNSGGGSPAQELPAIRTPADIYRVFVDVVNGEYVPRKSILKSRSRENSVCSDTSESSMADFDDRRALLRGVSCEEATCSDTGESIQEEEQPDHHQRRLLPSSGTPEAFSGTVIEKEFLSPSLTPHPAMAHPVLPTIPERKEVLSEVPEETTKRVSKFRAARLQQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K4R1</accession>
    <name>A0A9L0K4R1_EQUAS</name>
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        <recommendedName>
            <fullName evidence="5">H15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106837821</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the histone H1/H5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014707039.2">
        <property type="nucleotide sequence ID" value="XM_014851553.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K4R1"/>
    <dbReference type="Ensembl" id="ENSEAST00005057286.1">
        <property type="protein sequence ID" value="ENSEASP00005059798.1"/>
        <property type="gene ID" value="ENSEASG00005005121.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837821"/>
    <dbReference type="KEGG" id="eai:106837821"/>
    <dbReference type="GeneTree" id="ENSGT00940000163082"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000075">
        <property type="entry name" value="Histone H1 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005819">
        <property type="entry name" value="H1/H5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005818">
        <property type="entry name" value="Histone_H1/H5_H15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00538">
        <property type="entry name" value="Linker_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00624">
        <property type="entry name" value="HISTONEH5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00526">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51504">
        <property type="entry name" value="H15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="1" id="KW-0164">Citrullination</keyword>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="H15" evidence="5">
        <location>
            <begin position="35"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="94"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="148"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="189"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51504"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005059798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="284" mass="29550" checksum="E66EB4B349B4CE16" modified="2023-09-13" version="1">MSETAPAALPAPAPAEKTPVKKARKSASATKRKASGPPVSELIIKAVAASKERNGLSLAALKKALAATGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAATGEAKPKAKKAGAAKPKKASGVAKRPKKTPGTITPKKSARKTPRKVKKPAASKKVIKSAKKVNTAKPKKAAKSPAKAPKPKAAKPKGGKPKKAAPKKNTAWMLGRTPPCAMVTFPSSLFSSSSLRIASWRWRGMMRVFLLSRAALPANSKISAVKYSSTAAKYTGAPAPTRSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K4U3</accession>
    <name>A0A9L0K4U3_EQUAS</name>
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        <recommendedName>
            <fullName evidence="7">2'-deoxynucleoside 5'-phosphate N-hydrolase 1</fullName>
            <ecNumber evidence="7">3.2.2.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">c-Myc-responsive protein RCL</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 8" type="primary">DNPH1</name>
        <name evidence="7" type="synonym">RCL</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5'-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. Deoxyribonucleoside 5'-monophosphates containing purine bases are preferred to those containing pyrimidine bases.</text>
    </comment>
    <comment type="function">
        <text evidence="6">Part of a nucleotide salvage pathway that eliminates epigenetically modified 5-hydroxymethyl-dCMP (hmdCMP) in a two-step process entailing deamination to cytotoxic 5-hydroxymethyl-dUMP (hmdUMP), followed by its hydrolysis into 5-hydroxymethyluracil (hmU) and 2-deoxy-D-ribose 5-phosphate (deoxyribosephosphate). Catalyzes the second step in that pathway, the hydrolysis of the N-glycosidic bond in hmdUMP, degrading this cytotoxic nucleotide to avoid its genomic integration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>5-hydroxymethyl-dUMP + H2O = 5-hydroxymethyluracil + 2-deoxy-D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:77099"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16964"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="ChEBI" id="CHEBI:90409"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:77100"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a purine 2'-deoxyribonucleoside 5'-phosphate + H2O = a purine nucleobase + 2-deoxy-D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:51132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:26386"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="ChEBI" id="CHEBI:142198"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a pyrimidine 2'-deoxyribonucleoside 5'-phosphate + H2O = a pyrimidine nucleobase + 2-deoxy-D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:57852"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:26432"/>
            <dbReference type="ChEBI" id="CHEBI:62877"/>
            <dbReference type="ChEBI" id="CHEBI:142209"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1 7">Monomer and homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the 2'-deoxynucleoside 5'-phosphate N-hydrolase 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="7"/>
    <dbReference type="RefSeq" id="XP_014692893.1">
        <property type="nucleotide sequence ID" value="XM_014837407.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K4U3"/>
    <dbReference type="Ensembl" id="ENSEAST00005069923.1">
        <property type="protein sequence ID" value="ENSEASP00005056360.1"/>
        <property type="gene ID" value="ENSEASG00005029866.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106828727"/>
    <dbReference type="KEGG" id="eai:106828727"/>
    <dbReference type="CTD" id="10591"/>
    <dbReference type="GeneTree" id="ENSGT00390000001216"/>
    <dbReference type="OrthoDB" id="18087at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070694">
        <property type="term" value="F:5-hydroxymethyl-dUMP N-hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009159">
        <property type="term" value="P:deoxyribonucleoside monophosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030855">
        <property type="term" value="P:epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043174">
        <property type="term" value="P:nucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006195">
        <property type="term" value="P:purine nucleotide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.450:FF:000019">
        <property type="entry name" value="2'-deoxynucleoside 5'-phosphate N-hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03036">
        <property type="entry name" value="Nuc_phosphate_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051239">
        <property type="entry name" value="2'-dNMP_N-hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028607">
        <property type="entry name" value="DNPH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007710">
        <property type="entry name" value="Nucleoside_deoxyribTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15364">
        <property type="entry name" value="2'-DEOXYNUCLEOSIDE 5'-PHOSPHATE N-HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15364:SF0">
        <property type="entry name" value="2'-DEOXYNUCLEOSIDE 5'-PHOSPHATE N-HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05014">
        <property type="entry name" value="Nuc_deoxyrib_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52309">
        <property type="entry name" value="N-(deoxy)ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <begin position="16"/>
            <end position="22"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="120"/>
            <end position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared between homodimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005056360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="166" mass="18298" checksum="605387E7FC5B6BCD" modified="2023-09-13" version="1">MAAARERGEPGRRALYFCGSIRGGRDDRALYERIVSRLRRFGTVLTEHVAAPDLGVHGEEVAGGDKLIHERDLAWLQQADVVVAEVTQPSLGVGYELGRAVALNKRILCLFRPQSGRVLSAMIRGAADGLRFQVWDYEEGEVEAMLDRYFEADPSEEVAASPEPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K599</accession>
    <name>A0A9L0K599_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106833358</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K599"/>
    <dbReference type="Ensembl" id="ENSEAST00005048844.1">
        <property type="protein sequence ID" value="ENSEASP00005057532.1"/>
        <property type="gene ID" value="ENSEASG00005025502.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160602"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15226">
        <property type="entry name" value="7tmA_OR4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000012">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050427">
        <property type="entry name" value="Olfactory_Receptors"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48002">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="26"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="205"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="237"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="270"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="41"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005057532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="312" mass="35250" checksum="5AF4FF0A5850675A" modified="2023-09-13" version="1">MYEANYSLLSEFVFLGLSTSRPVQHFLLAFSTVLYITIVLGNLIVVFAVTFDHHLHSLMYFLLANLSFIDLCFSTLTVPKMIYDLYSGHKTISFQGCIIQIFVLHVLGGSEMVLLIAMALDQYVAICKLLHYLTVMSPRMCILLLSSAWAIGLIHSVTQLAFAVHLPFCGPNEIDSFYCDLPWFIKLACTDTYRMEFMVTANSGFISMGTFFLLIVSYIFLLVTLQKCSSGCFSKDLSTLSAHITVVILFFGPCIFVYVWPFPAVSVDKFLAILDFMITPILNPAIYTLRSKDMKMAMRRLNSQLLSLRKIS</sequence>
</entry>
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    <accession>A0A9L0K5N1</accession>
    <name>A0A9L0K5N1_EQUAS</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Zhao"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Guo"/>
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                <person name="Yang"/>
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                <person name="Tian"/>
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                <person name="Gao"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
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                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="Yue"/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the intermediate filament family.</text>
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        <property type="gene ID" value="ENSEASG00005028172.1"/>
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        <property type="component" value="Chromosome 22"/>
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        <property type="entry name" value="Type II keratin 23"/>
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    </dbReference>
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        <property type="entry name" value="Single helix bin"/>
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        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
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    </dbReference>
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        <property type="entry name" value="IF_rod_dom"/>
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    <dbReference type="InterPro" id="IPR032444">
        <property type="entry name" value="Keratin_2_head"/>
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    <dbReference type="InterPro" id="IPR003054">
        <property type="entry name" value="Keratin_II"/>
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    <dbReference type="PANTHER" id="PTHR45616">
        <property type="entry name" value="GATA-TYPE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616:SF52">
        <property type="entry name" value="KERATIN, TYPE II CUTICULAR HB3"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Keratin_2_head"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR01276">
        <property type="entry name" value="TYPE2KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
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        <location>
            <begin position="47"/>
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        </location>
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        <location>
            <begin position="239"/>
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    </evidence>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002494">
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    </dbReference>
    <dbReference type="Pfam" id="PF13885">
        <property type="entry name" value="Keratin_B2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="216" mass="22537" checksum="DC5949766D14EA2B" modified="2023-09-13" version="1">MKNVVKDLIDNKYAIKGTSCVGFVCQPICSCPASCESSTGQSPCLLPSSQPSCSESASCQPKCLHLLPLTMTHSCCSSCCQPTCCQPTCSESSCCGQTCSQSSCCQTTCYQPTSVTSCRLSCCPAPCCQPTCSESSCCGQTCSQSSCCQPCCPPACCQTICCQPACSGPVYCRRTCYHPTCVCLPCCQAQSCGSSCCQPCCRPACCESSCCRPSCC</sequence>
</entry>
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    <accession>A0A9L0K9F9</accession>
    <name>A0A9L0K9F9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">alkaline phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.1</ecNumber>
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        <name type="common">Donkey</name>
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    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
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                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="5">Binds 1 Mg(2+) ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0K9F9"/>
    <dbReference type="Ensembl" id="ENSEAST00005073788.1">
        <property type="protein sequence ID" value="ENSEASP00005061473.1"/>
        <property type="gene ID" value="ENSEASG00005029311.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183063"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596:SF30">
        <property type="entry name" value="INTESTINAL-TYPE ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040386464" description="alkaline phosphatase" evidence="7">
        <location>
            <begin position="21"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="4">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="361"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="437" mass="47120" checksum="7B53C38D9C45DB47" modified="2023-09-13" version="1">MQGAWVLLLLLGLRLRLSLGVIPVEEEDPAFWNRQAAQALDTAKKLQPIQTAAKNLIIFLGDGMGVPTVTATRILKAQMDGKLGPETPLAMDHFPYLALSKVRARGAGHSAGTATAYLCGVKANYQTIGVIWGRVGVVTTTTRVQHASPAGTYAHTVNRSWYSDAEMPDEALNDGCQDIARQLISNMDIDVILGGGRKYMFPKGTPDPEYPNDASQNGTRVDQRNLVQEWLAKYQGAQYVWNRTALIQASQDPSVTHLMGLFEPADLKYEAQRDLTLDPSLTEMTEAALRVLSRNPSGFYLFVEGLAPSKASDGKAYTSILYGNGPGYVLSGGSRPDVNESQSMDPAYKQQAAVPLSSETHGGEDVAVFARGPQAHLVHGVQEQTFVAHVMAFAACLEPYTACDLSPPAHIHRQPAAGSLSLQLLVGALLLLLSATH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K9R0</accession>
    <name>A0A9L0K9R0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Visual system homeobox 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Transcription factor VSX1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">VSX1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K9R0"/>
    <dbReference type="Ensembl" id="ENSEAST00005055692.1">
        <property type="protein sequence ID" value="ENSEASP00005062099.1"/>
        <property type="gene ID" value="ENSEASG00005030814.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160793"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000065">
        <property type="entry name" value="Visual system homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023339">
        <property type="entry name" value="CVC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051775">
        <property type="entry name" value="Homeobox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24323">
        <property type="entry name" value="CEH-10 HOMEODOMAIN-CONTAINING HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24323:SF3">
        <property type="entry name" value="VISUAL SYSTEM HOMEOBOX 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51496">
        <property type="entry name" value="CVC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 13 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 13 14" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 13 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0844">Vision</keyword>
    <feature type="domain" description="Homeobox" evidence="16">
        <location>
            <begin position="162"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="CVC" evidence="17">
        <location>
            <begin position="224"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="13">
        <location>
            <begin position="164"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="112"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="294"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="112"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="159"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00041737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005062099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="365" mass="38782" checksum="C8D5401544FA1117" modified="2023-09-13" version="1">MTGRDALSDGRSRSRALVPGGSPTGPRPRGFAITDLLGLEADLPSPAGPGPGSGCEGPAAAACPDPGLSGPCLARGALPLGLGLLCGFSARPPVAARAPCLLLADVPFLPPEGPEPAAPQAPSRPPPALGRQKRSESVSTSDEDSPSEDRSELKASHTPSRRKRRRHRTVFTAHQLEELEKAFSEAHYPDVYAREMLAVKTELPEDRIQVWFQNRRAKWRKREKRWGGSSVMAEYGLYGAMVRHCIPLPDSVLNSTDGGLVGSCAPWLLGMHKKSIGMIRKPGSEEKMAGLWDSDHFKEGSSPSQAGPQKRSDEGSPENGLEDVATDLSSSARQETKRAYQGASSQGCSDSEGLEGLLPGRVGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KCY5</accession>
    <name>A0A9L0KCY5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Tudor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">LOC106838049</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell projection</location>
            <location evidence="5">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
            <location evidence="1">Z line</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic granule</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Cajal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="17">Nucleus</location>
            <location evidence="17">Gem</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SMN family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KCY5"/>
    <dbReference type="Ensembl" id="ENSEAST00005062717.1">
        <property type="protein sequence ID" value="ENSEASP00005062674.1"/>
        <property type="gene ID" value="ENSEASG00005018000.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153352"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
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    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0030137">
        <property type="term" value="C:COPI-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097504">
        <property type="term" value="C:Gemini of Cajal bodies"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0030426">
        <property type="term" value="C:growth cone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007409">
        <property type="term" value="P:axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033120">
        <property type="term" value="P:positive regulation of RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010975">
        <property type="term" value="P:regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22852">
        <property type="entry name" value="SMN_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22851">
        <property type="entry name" value="SMN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20398">
        <property type="entry name" value="Tudor_SMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000110">
        <property type="entry name" value="Survival motor neuron protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000038">
        <property type="entry name" value="Survival of motor neuron-related-splicing factor 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040424">
        <property type="entry name" value="Smn1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047313">
        <property type="entry name" value="SMN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049481">
        <property type="entry name" value="SMN_G2-BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010304">
        <property type="entry name" value="SMN_Tudor"/>
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    <dbReference type="InterPro" id="IPR002999">
        <property type="entry name" value="Tudor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047298">
        <property type="entry name" value="Tudor_SMN_eumet"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39267:SF1">
        <property type="entry name" value="SURVIVAL MOTOR NEURON PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39267">
        <property type="entry name" value="SURVIVAL MOTOR NEURON-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20636">
        <property type="entry name" value="SMN_G2-BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06003">
        <property type="entry name" value="SMN_Tudor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20635">
        <property type="entry name" value="SMN_YG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="10" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="14" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="13" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Tudor" evidence="19">
        <location>
            <begin position="159"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="126"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="205"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="206"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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    <evidence type="ECO:0000256" key="9">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00034695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005062674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="362" mass="38645" checksum="FA2B0AC46CBDF125" modified="2023-09-13" version="1">MAMGGSGGGPEQEDSVLFRRGTGQVRSRPALPASLPPGPALLPRPLAGPGRSTACGAAARRFPHVGGPAASVFPSAAWAPCGLRAEALPQLGRPLSDDSDIWDDTALIKAYDKAVASFKHALKNGDISEASDKPKGTPKRKPAKKNKNQKKNPTTPLKQWKVGDKCCAIWSEDGCIYPATIASVDFRRETCVVVYTGYGNREEQNLSDLLSPTSEVANNTEQSAPENESESQISTDESENPSRSPGEKPNNIRSKAAPWSSFLPPPPPMPTPGLGPGKPGLKFNGPPPPPPPLPPHFLSCWLPPFPSGPPIIPPPPPICPDSLEDADALGSMLISWYMSGYHTGYYMGFKQNQKEGRCSHFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KEP9</accession>
    <name>A0A9L0KEP9_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Spectrin repeat containing nuclear envelope family member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SYNE3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Nucleus outer membrane</location>
            <topology evidence="7">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the nesprin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070370364.1">
        <property type="nucleotide sequence ID" value="XM_070514263.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070370365.1">
        <property type="nucleotide sequence ID" value="XM_070514264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0KEP9"/>
    <dbReference type="Ensembl" id="ENSEAST00005044979.1">
        <property type="protein sequence ID" value="ENSEASP00005061007.1"/>
        <property type="gene ID" value="ENSEASG00005021497.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106832629"/>
    <dbReference type="CTD" id="161176"/>
    <dbReference type="GeneTree" id="ENSGT00440000039367"/>
    <dbReference type="OMA" id="ACCLEDQ"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034993">
        <property type="term" value="C:meiotic nuclear membrane microtubule tethering complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005640">
        <property type="term" value="C:nuclear outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007097">
        <property type="term" value="P:nuclear migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000237">
        <property type="entry name" value="Spectrin repeat containing nuclear envelope family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000417">
        <property type="entry name" value="Spectrin repeat containing nuclear envelope family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.60:FF:000447">
        <property type="entry name" value="Spectrin repeat containing nuclear envelope family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.60">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012315">
        <property type="entry name" value="KASH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052403">
        <property type="entry name" value="LINC-complex_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018159">
        <property type="entry name" value="Spectrin/alpha-actinin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057932">
        <property type="entry name" value="Spectrin_SYNE1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057933">
        <property type="entry name" value="SYNE3_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47535">
        <property type="entry name" value="MUSCLE-SPECIFIC PROTEIN 300 KDA, ISOFORM G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47535:SF2">
        <property type="entry name" value="NESPRIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10541">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25803">
        <property type="entry name" value="Spectrin_SYNE1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25804">
        <property type="entry name" value="SYNE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01249">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00150">
        <property type="entry name" value="SPEC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46966">
        <property type="entry name" value="Spectrin repeat"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51049">
        <property type="entry name" value="KASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 8 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="8">
        <location>
            <begin position="1"/>
            <end position="925"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="926"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="topological domain" description="Perinuclear space" evidence="8">
        <location>
            <begin position="947"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="domain" description="KASH" evidence="11">
        <location>
            <begin position="917"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="295"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="975" mass="111724" checksum="CE28018DA8CD4798" modified="2023-09-13" version="1">MTQQPQEGFDRSVEDARQWMKAVQERLQISDNTQGPRAALEARLRETEKICQLEPEGRVKLDLALRAAEALLACCPEDQKPEILARLRDIKAQWEETVTYMTHCHSRIEWVWLHWSEYLLARDDFYRWFQKMVVALEPPVELQLGLKEKQWQLSHAQVLLHNVDNQAVLLDRLLEEAGSLFNRIGDPSVDEDAQKRMKAEYDAVKAKAQDRVDLLEQVAREHEHFQASVDEFQLWLKAVVEKVAGCLGQNCKLTTQHRLSALQDIAKDFPRGDESLKRLEEQAVGVIQNTSPLGAEKITEELEEMRKVLEKLRLLWEEEEGRLQGLLKSKGACEQQRRQLEAELGEFRKGLQRLAEEGLEPQAKAGTEDELVACWRLYSATRAALAAEEPRVDRLQAQLKELIAFPQDLQPLSDGVVAAIQEYQSMKGKSTRLRNATGTELWQRFQRPLQDLQLWKALAQRLLDVTTSLPDLPSIHTFLPQIEAALAESSRLKEPLTMLQLRKDLLSSVFGQERATALLEQVASAVRDRDLLLNSLLQRKSKLQSLLAQHKDFGAAFEPLQRKLSDLQVRVQAKNGLQRDLPGKQAQLSRLQGLQEDGLDLGVQVEAVRPLIQGNSNHQHKMDQLSADYQALQRFLEDLVDGCQQSVREHCTFSHKLLELRQWIAVVTQKLESHLGDTGPWDAQAREAEVERLLAEFPEKEAQLPLIKAHGWLVMEKSSLEGAAVVQEELEELEEAWRALRLLEESLLSLIRNRQLQRTEVDLGKKTIFTNNIPKPGFLITPTEPIARYRRQAGLLQEEGGSQEDFSQLLRNFEQWLQLENSKLVRIIAMRTATAKDLRTREMKLQELEARVPEGQHLFENLLRLGPARETSEELEDLRYRWMLYKSKLKDSSHLLTQSSPGETTGFQKSRRWRGLGALFRKVCCVALPLQLLLLLFLLLLFLLPVGEEDRSCTLANNFARSFMLMLRYDGPPPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KUG5</accession>
    <name>A0A8C4KUG5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Dishevelled binding antagonist of beta catenin 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the dapper family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KUG5"/>
    <dbReference type="Ensembl" id="ENSEAST00005000643.2">
        <property type="protein sequence ID" value="ENSEASP00005000537.2"/>
        <property type="gene ID" value="ENSEASG00005000507.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183181"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900108">
        <property type="term" value="P:negative regulation of nodal signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024843">
        <property type="entry name" value="Dapper"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15919:SF13">
        <property type="entry name" value="DAPPER HOMOLOG 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15919">
        <property type="entry name" value="DAPPER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15268">
        <property type="entry name" value="Dapper"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="118"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="229"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="275"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="436"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="529"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="303"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="453"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="593"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="627"/>
            <end position="646"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000537.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="675" mass="70907" checksum="1B9D10A3DD90AB97" modified="2023-09-13" version="2">IGAAVSKNTATRPPNAGLLRLSMGKKIQHGNRLRRQDVGLKTHLDQLDQQISELQLDVRRTSSEAPDSDSRPSSGFYELSDGGSCSLSTSCTSVCSDRMSSSLSVLLPAAPKTRLGVGDCRPRSADETTVCGTPLPTWGPQATEEGASRPRPVSTGDLERVRPAEVGLQKASADPKSTPLLCHGMDLPPHMLDPKYQRDLVSKGGKEVYPYPSPLHAVALQSPLFALTKETPQGDGHSPPSKALPGRAGLSSIQTGPVLEAGPAGAYIDRLLRLRGRGTPPRGSVGEQGPPRCEVSPSQQELSGRRVDSEDHLEKLVCTPGRAEVGGTAQRGNASGGILEQHPLVDTPHPSILPEEGCNPSNGSSRGENIPASPLLGCGRAQQSALDGCKGKARSPTERTGRESPALAPGHCGRPLFVASRASPTGLRTSLPKTKATKIRRGTSDKALRSGRQPPPWGALAVPPLPPEWGSVLRRRPTLAGDAPGRSCSEASLYPVPCFVPLLVARQEGHQASAQALFPFAAAPLVAAPGDARRKQRRWQSSVEISARARPLSIPDPSPAARRGGGPRPRPPRPRQDAPSGSESDASEGPSVLPSTIAESSGDESSTSDCEAGGRGRGGGLAWPRGAPQPSPRAPAGARPPLPPVPKLCRIKASKALKKKIRRFQPATLKVMTMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KV71</accession>
    <name>A0A8C4KV71_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA methyltransferase 3 like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">DNMT3L</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KV71"/>
    <dbReference type="Ensembl" id="ENSEAST00005002290.2">
        <property type="protein sequence ID" value="ENSEASP00005002073.2"/>
        <property type="gene ID" value="ENSEASG00005001632.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162228"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035098">
        <property type="term" value="C:ESC/E(Z) complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141068">
        <property type="term" value="P:autosome genomic imprinting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060718">
        <property type="term" value="P:chorionic trophoblast cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044726">
        <property type="term" value="P:epigenetic programing of female pronucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007141">
        <property type="term" value="P:male meiosis I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090310">
        <property type="term" value="P:negative regulation of DNA methylation-dependent heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044027">
        <property type="term" value="P:negative regulation of gene expression via chromosomal CpG island methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048599">
        <property type="term" value="P:oocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001890">
        <property type="term" value="P:placenta development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048863">
        <property type="term" value="P:stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141196">
        <property type="term" value="P:transposable element silencing by piRNA-mediated DNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025766">
        <property type="entry name" value="ADD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040552">
        <property type="entry name" value="DNMT3_ADD_GATA1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049554">
        <property type="entry name" value="DNMT3_ADD_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23068:SF13">
        <property type="entry name" value="DNA (CYTOSINE-5)-METHYLTRANSFERASE 3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23068">
        <property type="entry name" value="DNA CYTOSINE-5- -METHYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17980">
        <property type="entry name" value="ADD_DNMT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21255">
        <property type="entry name" value="DNMT3_ADD_GATA1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51533">
        <property type="entry name" value="ADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="6">
        <location>
            <begin position="41"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005002073.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="386" mass="43976" checksum="5F8A789D599789ED" modified="2023-09-13" version="2">MAAAPVLDLEEEPSMDIILVDSSELSSPPSPGPSRDLIEYEVKVNQRDIEDMCICCGSFQVHTQHPLFEGGMCTACKDKFLSCLFLYDDDGNQSYCSICCSGETLLICENPDCTRCYCFECVDTLVSPGTSEKVQAMSNWVCFLCLPFPRSGLLQRRRKWRGWLKAFYDQEAESPLEMYKTVPVWKREPVRVLSLFGDIKKELTSLGFLENGSDPGRLKHLDDVTNTVRRDVEEWGPFDLVYGSTPPLGHACDHPPGWYLFQFHRVLQYARPRPGSPQAFFWMFVDNLVLTEDDRAVATRFLETDPVTIQDVCGRAVRNAVHVWSNIPAVKSRHSALFSQEESFLRAQDRQRAKPPARGPAKLVKNCFLPLREYFKYFSTEFTSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KXD1</accession>
    <name>A0A8C4KXD1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ARF like GTPase 5C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ARL5C</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044600576.1">
        <property type="nucleotide sequence ID" value="XM_044744641.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070339252.1">
        <property type="nucleotide sequence ID" value="XM_070483151.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070339253.1">
        <property type="nucleotide sequence ID" value="XM_070483152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KXD1"/>
    <dbReference type="Ensembl" id="ENSEAST00005002699.2">
        <property type="protein sequence ID" value="ENSEASP00005002452.2"/>
        <property type="gene ID" value="ENSEASG00005001922.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106826373"/>
    <dbReference type="CTD" id="390790"/>
    <dbReference type="GeneTree" id="ENSGT00940000163066"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903292">
        <property type="term" value="P:protein localization to Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04153">
        <property type="entry name" value="Arl5_Arl8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000294">
        <property type="entry name" value="ADP-ribosylation factor-like protein 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024156">
        <property type="entry name" value="Small_GTPase_ARF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11711">
        <property type="entry name" value="ADP RIBOSYLATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
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    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Membrane</location>
            <topology evidence="1 6">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the OST5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KZF4"/>
    <dbReference type="Ensembl" id="ENSEAST00005002180.2">
        <property type="protein sequence ID" value="ENSEASP00005001974.1"/>
        <property type="gene ID" value="ENSEASG00005001558.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010089"/>
    <dbReference type="OMA" id="WLFVYEV"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007915">
        <property type="entry name" value="TMEM258/Ost5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13636">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 258"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05251">
        <property type="entry name" value="Ost5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="15"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="79" mass="9113" checksum="A6DF6535AD623D79" modified="2022-01-19" version="1">MELEAMSRYTCPGNLAVFLHLTMELLAIGMFFTTWLFVYEVSSTKYIQDIHKELLISLVALLFTDFGALFLLLWVGIYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KZV4</accession>
    <name>A0A8C4KZV4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Molecular chaperone MKKS</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">MKKS</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Probable molecular chaperone that assists the folding of proteins upon ATP hydrolysis. Plays a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. May play a role in protein processing in limb, cardiac and reproductive system development. May play a role in cytokinesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of a complex composed at least of MKKS, BBS10, BBS12, TCP1, CCT2, CCT3, CCT4, CCT5 and CCT8. Interacts with STUB1. Interacts with BBS2 (via coiled coil domain). Interacts with CCDC28B. Interacts with BBS12. Interacts with SMARCC1, a component of the SWI/SNF complexes; the interaction takes place predominantly in the cytoplasm and may modulate SMARCC1 location. Interacts with DLEC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KZV4"/>
    <dbReference type="Ensembl" id="ENSEAST00005002377.2">
        <property type="protein sequence ID" value="ENSEASP00005002155.2"/>
        <property type="gene ID" value="ENSEASG00005001688.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007214"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902636">
        <property type="term" value="C:kinociliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031514">
        <property type="term" value="C:motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014824">
        <property type="term" value="P:artery smooth muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048854">
        <property type="term" value="P:brain morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021987">
        <property type="term" value="P:cerebral cortex development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050910">
        <property type="term" value="P:detection of mechanical stimulus involved in sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060324">
        <property type="term" value="P:face development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045444">
        <property type="term" value="P:fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021766">
        <property type="term" value="P:hippocampus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030837">
        <property type="term" value="P:negative regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038108">
        <property type="term" value="P:negative regulation of appetite by leptin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045776">
        <property type="term" value="P:negative regulation of blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905515">
        <property type="term" value="P:non-motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045494">
        <property type="term" value="P:photoreceptor cell maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040018">
        <property type="term" value="P:positive regulation of multicellular organism growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060296">
        <property type="term" value="P:regulation of cilium beat frequency involved in ciliary motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051492">
        <property type="term" value="P:regulation of stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902140">
        <property type="term" value="P:response to inositol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007608">
        <property type="term" value="P:sensory perception of smell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035176">
        <property type="term" value="P:social behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007286">
        <property type="term" value="P:spermatid development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021756">
        <property type="term" value="P:striatum development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042311">
        <property type="term" value="P:vasodilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000016">
        <property type="entry name" value="McKusick-Kaufman syndrome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000011">
        <property type="entry name" value="McKusick-Kaufman/Bardet-Biedl syndromes putative chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028790">
        <property type="entry name" value="MKKS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46787:SF1">
        <property type="entry name" value="MOLECULAR CHAPERONE MKKS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46787">
        <property type="entry name" value="SYNDROMES PUTATIVE CHAPERONIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00059081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00064363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005002155.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="570" mass="61989" checksum="7AD5F4AB01F3AF7C" modified="2023-09-13" version="2">MSRLEAKKPSLCKREPLTSERVRATLSVLKGIVTSCYGPSGRLKQLHNGLGGCVCTTSQSSALLANLSVTHPILKILTTAMQNHVSCFSDCGLFTAILCCNLIENVQRIGLTPTTVMKLNKHLLRLCISYLKSEVCGCRIPVDFSSTQILLCLVRSILTSKPACMLTRKEIDHVSALILRAFLLTIPENTEDHIILGKSIIVPLKGQRVIDSTVLPGILIEMSEVQFMKLLPVKKSGSLKVALFCASLSGDLSDTGEGTVVVSYGVSLENAVLDQLLNLGRQLVSDHVDLVMCQKVIHPSLKQFLSMHHVIAIDRVGVALMEPLSKVTGTRPIGSLGSVSPSTYGSVKDWCTAKFGFKHFFHLIPNEATVCSLLLCNRSDTAWDELKVTCQTALHVLQLTLKEPSVLLGGGCTETHLAAYIRHKTCNEPESILKDEGCTQTELQLITEAFCSALESLAGSLEHDGGEILTDLKYGHFWSIQADSPSIVNWSNLLSRCGCGLCNSQEELSWSFLRSTCHPFAPQTCLPHEAAGSANNLTLDCFTAKLNGLQVAVETANLILDLSYVIEDKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KZV7</accession>
    <name>A0A8C4KZV7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">TNF receptor-associated factor</fullName>
            <ecNumber evidence="13">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">TRAF5</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Mediates activation of NF-kappa-B and probably JNK. Seems to be involved in apoptosis. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TNF receptor-associated factor family. A subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="13"/>
    <dbReference type="RefSeq" id="XP_014690310.1">
        <property type="nucleotide sequence ID" value="XM_014834824.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KZV7"/>
    <dbReference type="Ensembl" id="ENSEAST00005003900.2">
        <property type="protein sequence ID" value="ENSEASP00005003560.2"/>
        <property type="gene ID" value="ENSEASG00005002734.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106827146"/>
    <dbReference type="KEGG" id="eai:106827146"/>
    <dbReference type="CTD" id="7188"/>
    <dbReference type="GeneTree" id="ENSGT00940000160954"/>
    <dbReference type="OrthoDB" id="1737200at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035631">
        <property type="term" value="C:CD40 receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031996">
        <property type="term" value="F:thioesterase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023035">
        <property type="term" value="P:CD40 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097400">
        <property type="term" value="P:interleukin-17-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048255">
        <property type="term" value="P:mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023019">
        <property type="term" value="P:signal transduction involved in regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033209">
        <property type="term" value="P:tumor necrosis factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03780">
        <property type="entry name" value="MATH_TRAF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16642">
        <property type="entry name" value="mRING-HC-C3HC3D_TRAF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.210.10:FF:000001">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000274">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000323">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000356">
        <property type="entry name" value="TNF receptor-associated factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.210.10">
        <property type="entry name" value="Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002083">
        <property type="entry name" value="MATH/TRAF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012227">
        <property type="entry name" value="TNF_rcpt-assoc_TRAF_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008974">
        <property type="entry name" value="TRAF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049342">
        <property type="entry name" value="TRAF1-6_MATH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027130">
        <property type="entry name" value="TRAF5_C3HC3D_RING-HC_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001293">
        <property type="entry name" value="Znf_TRAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131">
        <property type="entry name" value="TNF RECEPTOR ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10131:SF83">
        <property type="entry name" value="TNF RECEPTOR-ASSOCIATED FACTOR 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21355">
        <property type="entry name" value="TRAF-mep_MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02176">
        <property type="entry name" value="zf-TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015614">
        <property type="entry name" value="TRAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00061">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49599">
        <property type="entry name" value="TRAF domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50144">
        <property type="entry name" value="MATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50145">
        <property type="entry name" value="ZF_TRAF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="11 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9 13 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="16">
        <location>
            <begin position="45"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="TRAF-type" evidence="18">
        <location>
            <begin position="128"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="TRAF-type" evidence="18">
        <location>
            <begin position="183"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="MATH" evidence="17">
        <location>
            <begin position="403"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="14">
        <location>
            <begin position="128"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="TRAF-type" evidence="14">
        <location>
            <begin position="183"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="257"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR015614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003560.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="558" mass="64456" checksum="0BC6ED8F30B3A540" modified="2023-09-13" version="2">MAYSEEQGGVPCGFIRQNSSNSVSLDFEPNTEYRFVEQLEERYKCAFCHSVLHNPHQTGCGHRFCQHCILSLRELNAVPLCPVDKEVIKSQEVFKDNCCKREVLNLYVYCKNAPGCNAKIILGRYQDHLQQCLFQAVQCSNEHCQESVLRKNLKEHLSAYCQFREEKCLYCKKDVVVINLQNHEENMCPEYPVSCPNKCLQIIPRTEVDEHLAVCPEAEQDCPFKQYGCPVKDKRGNLQEHEHSALRDHMLLVLEKNLQLEKQISDLHRSLERKESKIQQLTETVNKFEKEFKQFAQLFGKNGSFLSNIQLLASHLDKSAWLEAQVRQLLQMANQQETKFDLRALMEAIDTVKQKITLLETHDQRIVVLEGETNKHDVHINIHKAQLNKNEERFKLLEGACYNGKLIWKVTDYKMKKREALEGHTVSIFSQPFYTSRCGYRLCARAYLNGDGSGKGTHLSLYFVVMRGEFDSLLQWPFRQRVTLMLLDQSGKKNHIMETFKADPNSSSFKRPDGEMNIASGCPRFVAHSTLENAKNTYIKDDTLFLKVAVDLTDLEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KZW8</accession>
    <name>A0A8C4KZW8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Small ubiquitin-related modifier 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">SUMO1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Covalently attached to KCNB1; UBE2I increases cross-linking with KCNB1 and PIAS1 decreases cross-links with KCNB1. Interacts with SAE2, RANBP2, PIAS1 and PIAS2. Interacts with PRKN. Covalently attached to a number of proteins such as IKFZ1, PML, RANGAP1, HIPK2, SP100, p53, p73-alpha, MDM2, JUN, DNMT3B and TDG. Also interacts with HIF1A, HIPK2, HIPK3, CHD3, EXOSC9, RAD51 and RAD52. Interacts with USP25 (via ts SIM domain); the interaction weakly sumoylates USP25. Interacts with SIMC1, CASP8AP2, RNF111 and SOBP (via SIM domains). Interacts with BHLHE40/DEC1. Interacts with RWDD3. Interacts with UBE2I/UBC9 and this interaction is enhanced in the presence of RWDD3. Interacts with MTA1. Interacts with SENP2. Interacts with HINT1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">PML body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ubiquitin family. SUMO subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KZW8"/>
    <dbReference type="Ensembl" id="ENSEAST00005003386.2">
        <property type="protein sequence ID" value="ENSEASP00005003097.1"/>
        <property type="gene ID" value="ENSEASG00005024896.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154319"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd16114">
        <property type="entry name" value="Ubl_SUMO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000092">
        <property type="entry name" value="Small ubiquitin-related modifier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022617">
        <property type="entry name" value="Rad60/SUMO-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046332">
        <property type="entry name" value="SUMO1_Ubl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10562">
        <property type="entry name" value="SMALL UBIQUITIN-RELATED MODIFIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11976">
        <property type="entry name" value="Rad60-SLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="16">
        <location>
            <begin position="203"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00040632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="284" mass="31245" checksum="519EC5364332E52F" modified="2022-01-19" version="1">SRSCVWQAGEAGRWLTKNVSSGVRSTGIQFRLFRKADEPGRETIEASGSAFRDAQGVREGGVWAVATVPNTNFGDRELSPGDGRGPRCSAALRRLSLSHSRDHQHSGARALPAGGFPPFSCLAFPSRPSLPRRVPEVTRGVAEVPPAAVLCCREERRICKPRSEVLRARGRCCAETPGEAAVTMSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGHSTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L414</accession>
    <name>A0A8C4L414_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">G-protein coupled receptor 61</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">GPR61</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Orphan G-protein coupled receptor. Constitutively activates the G(s)-alpha/cAMP signaling pathway. Shows a reciprocal regulatory interaction with the melatonin receptor MTNR1B most likely through receptor heteromerization. May be involved in the regulation of food intake and body weight.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Forms heterodimer with MTNR1B. Interacts with ARRB1 and ARRB2 in a spontaneous and agonist-independent manner; leading to the internalization of GPR61 in the endosomal compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070343091.1">
        <property type="nucleotide sequence ID" value="XM_070486990.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L414"/>
    <dbReference type="Ensembl" id="ENSEAST00005004331.2">
        <property type="protein sequence ID" value="ENSEASP00005003946.1"/>
        <property type="gene ID" value="ENSEASG00005002998.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005045792.1">
        <property type="protein sequence ID" value="ENSEASP00005050843.1"/>
        <property type="gene ID" value="ENSEASG00005002998.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106827426"/>
    <dbReference type="KEGG" id="eai:106827426"/>
    <dbReference type="CTD" id="83873"/>
    <dbReference type="GeneTree" id="ENSGT00950000182998"/>
    <dbReference type="OMA" id="WMETPRR"/>
    <dbReference type="OrthoDB" id="6117944at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990763">
        <property type="term" value="F:arrestin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0004930">
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                <person name="X. and Zhong"/>
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    <reference evidence="15" key="2">
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Calcium-regulated non-lysosomal thiol-protease.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase C2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044611243.1">
        <property type="nucleotide sequence ID" value="XM_044755308.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L9N5"/>
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        <property type="protein sequence ID" value="ENSEASP00005007830.2"/>
        <property type="gene ID" value="ENSEASG00005005708.2"/>
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    <dbReference type="GeneID" id="106842756"/>
    <dbReference type="KEGG" id="eai:106842756"/>
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    <dbReference type="GeneTree" id="ENSGT00940000155892"/>
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        <property type="component" value="Chromosome 21"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MIT_calpain7_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Calpain 7"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Calpain 7"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Calpain 7"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
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        <property type="entry name" value="Calpain_cysteine_protease"/>
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        <property type="entry name" value="Calpain_III"/>
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        <property type="entry name" value="MIT_dom"/>
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        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
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        <property type="entry name" value="CALPAIN-7"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5 13" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 13" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="14">
        <location>
            <begin position="260"/>
            <end position="540"/>
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        <location>
            <position position="290"/>
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    <feature type="active site" evidence="12 13">
        <location>
            <position position="458"/>
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    <feature type="active site" evidence="12 13">
        <location>
            <position position="478"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00007623"/>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LCC6</accession>
    <name>A0A8C4LCC6_EQUAS</name>
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        <submittedName>
            <fullName evidence="8">SOS Ras/Rac guanine nucleotide exchange factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SOS1</name>
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    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_044772280.2"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="HFD_SOS1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22915">
        <property type="entry name" value="HFD_SOS1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01261">
        <property type="entry name" value="PH_SOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00155">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06224">
        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000068">
        <property type="entry name" value="Son of sevenless homolog 1 (Drosophila)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000029">
        <property type="entry name" value="son of sevenless homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000015">
        <property type="entry name" value="son of sevenless homolog 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.840.10">
        <property type="entry name" value="Ras guanine-nucleotide exchange factors catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.870.10">
        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008937">
        <property type="entry name" value="Ras-like_GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000651">
        <property type="entry name" value="Ras-like_Gua-exchang_fac_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019804">
        <property type="entry name" value="Ras_G-nucl-exch_fac_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023578">
        <property type="entry name" value="Ras_GEF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001895">
        <property type="entry name" value="RASGEF_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036964">
        <property type="entry name" value="RASGEF_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055251">
        <property type="entry name" value="SOS1_NGEF_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23113:SF168">
        <property type="entry name" value="SON OF SEVENLESS HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00617">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00618">
        <property type="entry name" value="RasGEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22697">
        <property type="entry name" value="SOS1_NGEF_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00147">
        <property type="entry name" value="RasGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00229">
        <property type="entry name" value="RasGEFN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48366">
        <property type="entry name" value="Ras GEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00720">
        <property type="entry name" value="RASGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50009">
        <property type="entry name" value="RASGEF_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50212">
        <property type="entry name" value="RASGEF_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="6">
        <location>
            <begin position="200"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="4">
        <location>
            <begin position="443"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="N-terminal Ras-GEF" evidence="7">
        <location>
            <begin position="597"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="domain" description="Ras-GEF" evidence="5">
        <location>
            <begin position="780"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1019"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1112"/>
            <end position="1127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1193"/>
            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="1237"/>
            <end position="1249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1295"/>
            <end position="1308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007571.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1318" mass="150777" checksum="A555AF81559A950A" modified="2023-09-13" version="2">MQAQQLPYEFFSEENAPKWRGLLVPALKKVQGQVHPTLESSDDALQYVEELILQLLNMLCQAQPRSASDVEERVQKSFPHPIDKWAIADAQSAIEKRKRRNPLSLPVEKIHPLLKEVLGYKIDHQVSVYIVAVLEYISADILKLVGNYVRNIRHYEITKQDIKVAMCADKVLMDMFHQDVEDINILSLTDEEPSTSGEQTYYDLVKAFMAEIRQYIRELNLIIKVFREPFVSNSKLFSANDVENIFSRIVDIHELSVKLLGHIEDTVEMTDEGSPHPLVGSCFEDLAEELAFDPYESYARDILRPGFHDHFLSQLSKPGAALYLQSIGEGFKEAVQYVLPRLLLAPVYHCLHYFELLKQLEEKSEDQEDKECLKQAITALLNVQSGMEKICSKSLAKRRLSESACRFYSQQMKGKQLAIKKMNEIQKNIDGWEGKDIGQCCNEFIMEGTLTRVGAKHERHIFLFDGLMICCKSNHGQPRLPGASNAEYRLKEKFFMRKVQINDKDDTSEYKHAFEIILKDENSVIFSAKSAEEKNNWMAALISLQYRSTLERMLDVTMLQEEKEEQMRLPSADVYRFAEPDSEENIIFEENMQPKAGIPIIKAGTVIKLIERLTYHMYADPNFVRTFLTTYRSFCKPQELLSLIIERFEIPEPEPTEADRIAIENGDQPLSAELKRFRKEYIQPVQLRVLNVCRHWVEHHFYDFERDADLLQRMEEFIGTVRGKAMKKWVESITKIIQRKKIARDNGPGHNITFQSSPPTVEWHISRPGHIETFDLLTLHPIEIARQLTLLESDLYRAVQPSELVGSVWTKEDKEINSPNLLKMIRHTTNLTLWFEKCIVETENLEERVAVVNRIIEILQVFQELNNFNGVLEVVSAMNSSPVYRLDHTFEQIPSRQKKILEEAHELSEDHYKKYLAKLRSINPPCVPFFGIYLTNILKTEEGNPEVLKRHGKELINFSKRRKVAEITGEIQQYQNQPYCLRVESDIKRFFENLNPMGNSMEKEFTDYLFNKSLEIEPRNPKPLPRFPKKYSYPLKSPGVRPSNPRPGTMRHPTPLQQEPRKISYSRIPESETESTASAPNSPRTPLTPPPASGASSTTDVCSVFDSDHSSPFHSRSASVSSISLTKGTDEVPVPPPVPPRRRPESAPAESSPSKIMSKHLDSPPAIPPRQPTSKAYSPRYSISERTSISDPPESPPLLPPREPVRTPDVFSSSPLHLQPPPLGKKSDHGNAFFPNSPSPFTPPPPQTPSPHGTRRHLPSPPLTQEVDLHSIAGPPVPPRQSTSQHIPKLPPKTYKREHTHPSMHRDGPPLLENAHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LCD6</accession>
    <name>A0A8C4LCD6_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Protein phosphatase 2 regulatory subunit B''beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PPP2R3B</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">The B regulatory subunit might modulate substrate selectivity and catalytic activity, and might also direct the localization of the catalytic enzyme to a particular subcellular compartment.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LCD6"/>
    <dbReference type="Ensembl" id="ENSEAST00005010850.2">
        <property type="protein sequence ID" value="ENSEASP00005009990.2"/>
        <property type="gene ID" value="ENSEASG00005007065.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154659"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21507">
        <property type="entry name" value="PPP2R3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.220:FF:000001">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000628">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.230:FF:000001">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041534">
        <property type="entry name" value="EF-hand_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048855">
        <property type="entry name" value="P2R3A_B_D_EF-hand"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14095">
        <property type="entry name" value="PHOSPHATASE 2A REGULATORY SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14095:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B'' SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17958">
        <property type="entry name" value="EF-hand_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21161">
        <property type="entry name" value="P2R3B_EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="373"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00093310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009990.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="560" mass="64257" checksum="4AA1E4FEC15B2EF6" modified="2023-09-13" version="2">MERAEPGRPDRPAGRSCRPEPAGPGQAQPREQERERGAWPGPRRPTRADMRLRELSLRQDPDLRQELASLARGCDFVLPSRFKKRLKAFQQVQVQTKKEEPLPPASSQSIPTFYFPRGRPQGTVNVDAVIAKIERTFAQFPHERATMEDMGKVAKACGCPLYWKGPLFCGAGGERTGAVSVHKFVAMWRKVLQTCHDAAARFLHLLASPGCPYLVQDDFVPFLQDVVNTHPGLAFLKEASEFHSRYITTVIQRIFYTVNRSWSGRITCAELRRSTFLQNVALLEEEADINQLTEYFSYEHFYVIYCKFWELDTDHDLLIDSQDLARHNDHAISTKMIERIFSGAVTRGRKVQKEGKLSYADFVWFLISEEDKKTPTSIEYWFRCMDLDGDGALSMFELEYFYEEQCRRLDSMAIEALPFEDCLCQMLDLVKPQSEGKITLHDLKKCKLANVFFDTFFNIEKYLDHEQKEQVSLLRESESEGPELSDWEKYAAEEYDILVAEEAAGEPWEDGYEAELSPVDQKLCALRSPLAQRPFFEAPSALGAVELYEYECGDDHLQPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LDC5</accession>
    <name>A0A8C4LDC5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PAK2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Laurasiatheria</taxon>
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    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
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                <person name="Pan"/>
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                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
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                <person name="J."/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homodimer; disulfide-linked. Interacts with TXN/thioredoxin through its redox-active site. Interacts with transcriptional repressors ZBTB16, ZBTB32 and HDAC1. Interacts with DDIT4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the arrestin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014722519.1">
        <property type="nucleotide sequence ID" value="XM_014867033.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LFM5"/>
    <dbReference type="SMR" id="A0A8C4LFM5"/>
    <dbReference type="Ensembl" id="ENSEAST00005011465.2">
        <property type="protein sequence ID" value="ENSEASP00005010535.1"/>
        <property type="gene ID" value="ENSEASG00005007477.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106847645"/>
    <dbReference type="KEGG" id="eai:106847645"/>
    <dbReference type="CTD" id="10628"/>
    <dbReference type="GeneTree" id="ENSGT00940000158522"/>
    <dbReference type="OMA" id="TKKYFEV"/>
    <dbReference type="OrthoDB" id="2333384at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071228">
        <property type="term" value="P:cellular response to tumor cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030216">
        <property type="term" value="P:keratinocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000016">
        <property type="entry name" value="thioredoxin-interacting protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.640:FF:000017">
        <property type="entry name" value="thioredoxin-interacting protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011022">
        <property type="entry name" value="Arrestin_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050357">
        <property type="entry name" value="Arrestin_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188">
        <property type="entry name" value="ARRESTIN DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11188:SF14">
        <property type="entry name" value="THIOREDOXIN-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02752">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01017">
        <property type="entry name" value="Arrestin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Arrestin C-terminal-like" evidence="14">
        <location>
            <begin position="174"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00039479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM01017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005010535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="391" mass="43656" checksum="C7243077B212FEA1" modified="2022-01-19" version="1">MVMFKKIKSFEVVFNDPDKVFGCGEKVAGRVIVEVCEVTRVKAVRILACGVAKVVWMQGSQQCKQTSEYLRYEDTLLLEDQPAGENEMVIMRPGNRYEYKFGFELPEGPLGTSFKGKYGCVDYWVKAFLDRPSQPTQETKKNFEVMDLVDVNTPDLLVPVSAKKEKKVSCMFIPDGRVSVSARIDRKGFCEGDEISIHADFENTCSRIVIPKAAIVARHTYLANGQTKVLTEKLSSVRGNHIISGTCASWRGKSLRVQKIRPSILGCNILRVEYFLLIYVSIPGSKKVILDLPLVIGSRSGLSSRTSSMASQTSSEMSWVDLNIPDTPEAPPCYMDIIPEDHLLESPTTPLLDDTDSPQDSPIFMYAPEFKFMPPPTYTEVDPGVLNNNVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LGR8</accession>
    <name>A0A8C4LGR8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Integrin subunit alpha 10</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ITGA10</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014722543.2">
        <property type="nucleotide sequence ID" value="XM_014867057.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LGR8"/>
    <dbReference type="Ensembl" id="ENSEAST00005011416.2">
        <property type="protein sequence ID" value="ENSEASP00005010492.2"/>
        <property type="gene ID" value="ENSEASG00005007436.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106847657"/>
    <dbReference type="KEGG" id="eai:106847657"/>
    <dbReference type="CTD" id="8515"/>
    <dbReference type="GeneTree" id="ENSGT00940000158423"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034680">
        <property type="term" value="C:integrin alpha10-beta1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098639">
        <property type="term" value="F:collagen binding involved in cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01469">
        <property type="entry name" value="vWA_integrins_alpha_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.130:FF:000001">
        <property type="entry name" value="Integrin subunit alpha 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.130:FF:000004">
        <property type="entry name" value="Integrin subunit alpha 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1510:FF:000014">
        <property type="entry name" value="Integrin subunit alpha 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1530:FF:000007">
        <property type="entry name" value="Integrin subunit alpha 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.930:FF:000005">
        <property type="entry name" value="Integrin, alpha 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000012">
        <property type="entry name" value="Integrin, alpha 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF26">
        <property type="entry name" value="INTEGRIN ALPHA-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 17" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 17" id="KW-0401">Integrin</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <keyword evidence="3 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040527888" evidence="17">
        <location>
            <begin position="23"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="1121"/>
            <end position="1145"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="16">
        <location>
            <begin position="24"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="18">
        <location>
            <begin position="167"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="16">
        <location>
            <begin position="472"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="16">
        <location>
            <begin position="535"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="16">
        <location>
            <begin position="597"/>
            <end position="657"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005010492.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1167" mass="127940" checksum="72FD289193F6AB77" modified="2023-09-13" version="2">MELPLIPHLFLLLMFLTGLCFPFNLDVHRPRLFPGPPTAEFGYSVLQHVGGGRRWMLVGAPWDGPSGNRRGDIYRCPVGGSHNAPCAKGHLGDYPLGNSSRPAVNMHLGMSLLETDGDGGFMACAPLWSRACGSSVFSSGICARVDASFQLQGSLAPTAQRCPTYMDVVIVLDGSNSIYPWSEVRTFLRRLVGRLFIDPEQIQVGLVQYGESPVHEWSLGDFRTKEEVVRAARNLSRREGRETKTAQAIMVACTEGFSQSHGGRPEAARLLVVVTDGESHDGEELPTALKACEAGRVTRYGIAVLGHYLRRQRDPSSFLREIRAIASDPDERFFFNVTDEAALTDIVDALGDRIFGLEGSHGENESSFGLEMSQIGFSTHQLKDGILFGMVGAYDWGGSVLWLEEGHRIFPSRTALEDEFPPALQNHAAYLGYSVSSMLLRGGRRFFLSGAPRFRHRGKVIAFQLKKDGAMRVAQSLQGEQIGSYFGSELCPLDMDGDGTTDVLLVAAPMFLGPQNKETGRVYVYLVGQQSLLTLQGTLQPEPPQDARFGFAMGALPDLNQDGFADVAVGAPLEDGHHGALYLYHGTQSGVKPRPAQRIAAISMPQTLSYFGRSVDGRLDLDGDDLVDVAVGAQGAAILLSSRPIVHLAPTLDVTPPAISVVQRDCRRRGQEAACLSAALCFQVTSHTPGHWDRRFYMRFTASLDEWTAGARAAFDGSGQRLSSRRLRLSVGNVTCEQLHFHVLDTSDYLRPVALTVTFALDNTTKPGPVLDEASFTSIQKLVPFSKDCGPDNECITDLVLRVNMDIRGSRKDPFMVRGGRQKVLVSAALENRKENAYNTSLSLSFSRNLHLASFTPQRDSPVKVECTAPSSHARLCSVGHPVFQTGAKVTFQLEFEFSCSSLLSQVLVRLTATSNSLERNGTLQDNTAQTSAYIQYEPHLLFSSESTLHRYEVHPYGTLPVGPGPEFKTTLRVQNLGCYVISGLIISALLPAVAHGGNYFLSLSQVISNNASCTVHNLTEPPGPPLHPEELQHTSRLNGSNTRCQVVRCHLGRLAKGTEVSVGLLRLVHNEFFRRAKFKSLTVVSTFELGTEEGSVLQLTEASRWSESLLEVIQTRPVLISLWILIGSVLGGLLLLALLVFCLWKLGFFARKKIPKEEKREEKLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LN42</accession>
    <name>A0A8C4LN42_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Keratin-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106847391</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated proteins (KRTAP), which are essential for the formation of a rigid and resistant hair shaft through their extensive disulfide bond cross-linking with abundant cysteine residues of hair keratins. The matrix proteins include the high-sulfur and high-glycine-tyrosine keratins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with hair keratins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PMG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LN42"/>
    <dbReference type="Ensembl" id="ENSEAST00005012054.2">
        <property type="protein sequence ID" value="ENSEASP00005011087.2"/>
        <property type="gene ID" value="ENSEASG00005035665.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162109"/>
    <dbReference type="OMA" id="LYSGCHK"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007951">
        <property type="entry name" value="KRTAP_PMG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05287">
        <property type="entry name" value="PMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0416">Keratin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011087.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="175" mass="19373" checksum="9120B434BB3DEC16" modified="2023-09-13" version="2">MSYSCCSGNFSSHSLGSYLRYPGSFCGSSYPSNLVYSTGLCRPSTCQLGSSLYSGCHKTCWEPTRYQTSCVVSSPCQRSCYHTRTSSLCSPYGSTYTRPLGCRSSDSCCPGYRSRSSYSLGCGSSGFRPLHYGICGFPSLSSGSRFCHPTYFRFWNLQPSCYRPICGSDYYRFTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LNL0</accession>
    <name>A0A8C4LNL0_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LNL0"/>
    <dbReference type="Ensembl" id="ENSEAST00005014827.2">
        <property type="protein sequence ID" value="ENSEASP00005013655.1"/>
        <property type="gene ID" value="ENSEASG00005009537.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000186412"/>
    <dbReference type="OMA" id="DTEIMSY"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005013655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="77" mass="9168" checksum="A2CDE2E09A9CA5AB" modified="2022-01-19" version="1">MINNIKLDFPVPFIQLLGKNLAKIMNQGKGSHDHYLGLGYSRWHLILEKQCFLNACGNDTEIMSYFPFYFYFFLFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LPH2</accession>
    <name>A0A8C4LPH2_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Stabilizer of axonemal microtubules 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SAXO4</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="AlphaFoldDB" id="A0A8C4LPH2"/>
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        <location>
            <begin position="445"/>
            <end position="455"/>
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        <source>
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    <sequence length="463" mass="51449" checksum="FBC4F081ED26E978" modified="2023-09-13" version="2">MMGKLPLGSVSPYVKMSSGGCTDPLKFYATSYSVAYGREDFRPHMGSHKGTGYQSNFRPVVSYQAKVDTLDPPGPAMGKQVCDNLQTVTSQSYRPPEVSDGGYPLPWDLAKTSSGYSREKSNVTPLTKEVRKVHFDTPDYRPQAITGLEPRKVPLLHQQQDRGSLEWENARYGPHFMTSEYNSKYLKEPSQEPDLLQRRSIGAKEETGFTKESTRSPIVFQPPSQALPGDPVLLPSRSVTKSDFLPMTYPHGDELLPTLTRGSEWETTFSRGNERTLNPRVPPPSPEPSSMTRQFQPPQRMQQTNVSLLGQETVGSKEPTGFSLNSPSYVRSPYDPGMDNRYLNAYNQRYFENIPKGRDCEGWTRGGIQPQKPGGYTLNQSATHVEATPAPTESLRRQHPYVGRTLISADPLCRASHTSQQPLHRPQLSLRPGLANLMGQEPAPVAVPTPTPVGTPPTTFLMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LQX9</accession>
    <name>A0A8C4LQX9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Nitric oxide synthase</fullName>
            <ecNumber evidence="20">1.14.13.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">NOS2</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>2 L-arginine + 3 NADPH + 4 O2 + H(+) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H2O</text>
            <dbReference type="Rhea" id="RHEA:19897"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16480"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.39"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:19898"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>(6R)-L-erythro-5,6,7,8-tetrahydrobiopterin</name>
            <dbReference type="ChEBI" id="CHEBI:59560"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="20">Binds 1 FAD.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="20">Binds 1 FMN.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 20">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="19">Homodimer. Interacts with NHERF1. Interacts with GAPDH; induced by oxidatively-modified low-densitity lipoprotein (LDL(ox)). Interacts with S100A8 and S100A9 to form the iNOS-S100A8/9 transnitrosylase complex. Interacts with SPSB1, SPSB2 and SPSB4. Interacts with ELOC and CUL5 in the presence of SPSB1 or SPSB2 or SPSB4. Forms a complex with ASL, ASS1 and HSP90AA1; the complex regulates cell-autonomous L-arginine synthesis and citrulline recycling while channeling extracellular L-arginine to nitric oxide synthesis pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">Belongs to the NOS family.</text>
    </comment>
    <dbReference type="EC" id="1.14.13.39" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4LQX9"/>
    <dbReference type="Ensembl" id="ENSEAST00005016511.2">
        <property type="protein sequence ID" value="ENSEASP00005015177.2"/>
        <property type="gene ID" value="ENSEASG00005010108.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159752"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="term" value="F:nitric-oxide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:defense response to bacterium"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nitric Oxide Synthase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1230.10">
        <property type="entry name" value="Nitric Oxide Synthase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.440.10">
        <property type="entry name" value="Nitric Oxide Synthase,Heme Domain,Chain A domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001709">
        <property type="entry name" value="Flavoprot_Pyr_Nucl_cyt_Rdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050607">
        <property type="entry name" value="NOS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044943">
        <property type="entry name" value="NOS_dom_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044940">
        <property type="entry name" value="NOS_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044944">
        <property type="entry name" value="NOS_dom_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012144">
        <property type="entry name" value="NOS_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004030">
        <property type="entry name" value="NOS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036119">
        <property type="entry name" value="NOS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43410:SF4">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43410">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE OXYGENASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
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    <dbReference type="PRINTS" id="PR00371">
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        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56512">
        <property type="entry name" value="Nitric oxide (NO) synthase oxygenase domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
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        <property type="entry name" value="NOS"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 20" id="KW-0288">FMN</keyword>
    <keyword evidence="6 20 21" id="KW-0349">Heme</keyword>
    <keyword evidence="16 20 21" id="KW-0408">Iron</keyword>
    <keyword evidence="9 20 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 20" id="KW-0521">NADP</keyword>
    <keyword evidence="15 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="23">
        <location>
            <begin position="446"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="24">
        <location>
            <begin position="637"/>
            <end position="877"/>
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    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1045"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="21">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001950"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
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    </evidence>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022857"/>
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            <dbReference type="ARBA" id="ARBA00047419"/>
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            <dbReference type="ARBA" id="ARBA00054107"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00063867"/>
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    </evidence>
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            <dbReference type="PIRSR" id="PIRSR000333-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51384"/>
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    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005015177.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1064" mass="121209" checksum="53E9FF9E5A6875D1" modified="2023-09-13" version="2">WTPRRRCHRGGEGHQGVACKSKSCLAAVMNPKSMTRGPRDKPTPPDELLPHAIQFVNQYYNSFKEAKIEEHLARLEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCSTAQEMFEHICRHLRYSTNNGNIRSAITVFPQRSDGKHDFRIWNAQLIRYAGYQMPDGTIRGDPASVELTQLCIDLGWKPKYGRFDVMPLVLQADGRDPELFEIPPDLVLEVPMEHPKYEWFQELGLKWYALPAVANMLLEVGGLEFPGCPFNGWYMGTEIGVRDYCDAQRYNILEEVGRRMGLETHKLASLWKDRAVIEINVAVLHSFQKQNVTIMDHHSAAESFMKHMQNEYRSRGGCPADWVWLVPPISGSITPVFHQEMLNYVLSPFYYYQVEAWKTHVWQDEKQSPRRRQIRFKVLPVLFASMLMRKTMASRVRATILFATETGKSETLARDLGTLFSCAFNPKVLCMDEYQLRYLEEEQLLLVVTSTFGNGDSPGNGEKLKKSLFMLKKLTNKFRYAVFGLGSSMYPQFCAFAHDIDQKLSHLGASQLTPTGEGDELGGQEDAFRSWAVQTFKVTCETFNVRGKHQIQIPKLYTSNETWDPHHYRLVQDPQPLDLSKALSSMHAKNVFTMRLKSRQNLQSPKSSRTTLLIELSSEASQGLSYLPGEHLGVFPGNQPALVQGILERVEDGPAPDQPVRLETLDESGSYWVKDKRLPPCSLIQALTYFLDITTPPTQQLLRKLAQLATKEAERQRLETLCQPSEYNKWKLTNSPSFLEVLEEFPSLRVSAGFLLSQLPILKPRYYSISSSRDRTPMEVHLTVAVLTYRTRDGQGPLHHGVCSTWLSSLKPQDPVPCFVRSATGFRLPENPSHPCILVGPGTGIAPFRSFWLQRLHDAENRGLQGGRMTLVFGCRHPDEDHLYQEEMLEMARKGVLHEVHTAYSRLPGQPKVYVQDILRQQLVSEVLRVLHEEQGHIYVCGDVRMARDVAHTLKQLVAAKLSLSEEQVEDYFFQLKSQKRYHEDIFGAAVPYKLRKDGAAGQPSHPRAPSAPRKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LRA8</accession>
    <name>A0A8C4LRA8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Doublecortin domain containing 2B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">DCDC2B</name>
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    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LRA8"/>
    <dbReference type="Ensembl" id="ENSEAST00005017101.2">
        <property type="protein sequence ID" value="ENSEASP00005015722.1"/>
        <property type="gene ID" value="ENSEASG00005010951.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161946"/>
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        <property type="component" value="Chromosome 5"/>
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        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Doublecortin domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.230:FF:000011">
        <property type="entry name" value="Doublecortin domain containing 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.230">
        <property type="entry name" value="Doublecortin domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003533">
        <property type="entry name" value="Doublecortin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036572">
        <property type="entry name" value="Doublecortin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23004">
        <property type="entry name" value="DOUBLECORTIN DOMAIN CONTAINING 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23004:SF10">
        <property type="entry name" value="DOUBLECORTIN DOMAIN-CONTAINING PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03607">
        <property type="entry name" value="DCX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00537">
        <property type="entry name" value="DCX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89837">
        <property type="entry name" value="Doublecortin (DC)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50309">
        <property type="entry name" value="DC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Doublecortin" evidence="3">
        <location>
            <begin position="9"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Doublecortin" evidence="3">
        <location>
            <begin position="129"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="224"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="233"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="245"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005015722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="349" mass="37346" checksum="FA234996FDA07349" modified="2022-01-19" version="1">MASGSSAAKRVVVYRNGDSFSPGRRLVVTQRRFPTLDAFLCEVTSVVQAPLAVRALYTPCHGHPVTDLADLQNGGQYVAAGFERFRKLHYVPPGGKDPGGKSSRLPGPPVTHHSCDGALGCRLPAGSPCCIHVFRNGDLLSPPFSLKLSWAASKDWETVLKLLAEKVKLQSGAVCKLYTLEGLPLSAGEALVSGHYYVAVGEDEFKALPYLELLVPSPSLPRGCWHPPDPKSRAHKQRAHGHRAQATQPSPKESSQIEPSAFHARPQQAIQPSSILPTLSFPSGVKGVYRASHPRKETAGAQEVADDEDTWTEEPLDQRAAQIVEEDLSLENLPGAGAAISASAPAPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LRS5</accession>
    <name>A0A8C4LRS5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Clavesin-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Retinaldehyde-binding protein 1-like 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CLVS2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Required for normal morphology of late endosomes and/or lysosomes in neurons. Binds phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Forms a complex with clathrin heavy chain and gamma-adaptin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Early endosome membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014708421.1">
        <property type="nucleotide sequence ID" value="XM_014852935.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044613386.1">
        <property type="nucleotide sequence ID" value="XM_044757451.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LRS5"/>
    <dbReference type="SMR" id="A0A8C4LRS5"/>
    <dbReference type="Ensembl" id="ENSEAST00005014541.2">
        <property type="protein sequence ID" value="ENSEASP00005013387.1"/>
        <property type="gene ID" value="ENSEASG00005009336.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106838719"/>
    <dbReference type="KEGG" id="eai:106838719"/>
    <dbReference type="CTD" id="134829"/>
    <dbReference type="GeneTree" id="ENSGT00940000157632"/>
    <dbReference type="OMA" id="DEEPDYC"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080025">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007040">
        <property type="term" value="P:lysosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00170">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.20:FF:000001">
        <property type="entry name" value="Alpha-tocopherol transfer protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.525.10:FF:000002">
        <property type="entry name" value="Alpha-tocopherol transfer protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1200:FF:000001">
        <property type="entry name" value="Clavesin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1200">
        <property type="entry name" value="Alpha-tocopherol transfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.525.10">
        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011074">
        <property type="entry name" value="CRAL/TRIO_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174">
        <property type="entry name" value="ALPHA-TOCOPHEROL TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10174:SF73">
        <property type="entry name" value="CLAVESIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03765">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00180">
        <property type="entry name" value="CRETINALDHBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01100">
        <property type="entry name" value="CRAL_TRIO_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
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    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="14">
        <location>
            <begin position="96"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="287"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046144"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00063131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005013387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="327" mass="38000" checksum="FF40A5A222A4DB1C" modified="2022-01-19" version="1">MTHLQAGLSPETLEKARLELNENPDTLHQDIQEVRDMVITRPDIGFLRTDDAFILRFLRARKFHHFEAFRLLAQYFEYRQQNLDMFKSFKATDPGIKQALKDGFPGGLANLDHYGRKILVLFAANWDQSRYTLVDILRAILLSLEAMIEDPELQVNGFVLIIDWSNFTFKQASKLTPSMLRLAIEGLQDSFPARFGGIHFVNQPWYIHALYTVIRPFLKEKTRKRIFLHGNNLNSLHQLIHPEILPSEFGGMLPPYDMGTWARTLLDHEYDDDSEYNVDSYSMPVKEVEKELSPKSMKRSQSVVDPTVLKRMDKNEEENMQPLLSLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LSN3</accession>
    <name>A0A8C4LSN3_EQUAS</name>
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        <submittedName>
            <fullName evidence="11">Calpain 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CAPN14</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LSN3"/>
    <dbReference type="Ensembl" id="ENSEAST00005013449.2">
        <property type="protein sequence ID" value="ENSEASP00005012384.2"/>
        <property type="gene ID" value="ENSEASG00005008638.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160421"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000054">
        <property type="entry name" value="Calpain 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.380:FF:000001">
        <property type="entry name" value="Calpain-1 catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022682">
        <property type="entry name" value="Calpain_domain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022683">
        <property type="entry name" value="Calpain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF302">
        <property type="entry name" value="CALPAIN-14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01067">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00720">
        <property type="entry name" value="calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 9" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="10">
        <location>
            <begin position="43"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="active site" evidence="8 9">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="active site" evidence="8 9">
        <location>
            <position position="255"/>
        </location>
    </feature>
    <feature type="active site" evidence="8 9">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005012384.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="669" mass="76597" checksum="0C4EB1D7A2C781CB" modified="2023-09-13" version="2">MSLWPSFRGRWKLGRKDSTGGSSQLPKQDYEALQERCLRDSCLFEDDSFPATLSSIGSGPLLRKLPRRLQWRRPPELHSNPQFYSAKGKRLDLCQGVVGDCWFLAALQALTLHRDILSQVVPLNQSFTEKYAGIFQFWFWHFGKWVPVVIDDRLPVNEAGQLVFVSSTYKNLFWGALLEKAYAKLSGSYEDLQLGQVSEALVDFTGGVTVTINLAEVPGNLWDILTRATYSRTLIGCQTHSGKERVLENGLVGGHAYTLTGIRKVTWKHGPEYLVKLRNPWGKVEWKGDWSDSSSTWELLSPKEKILLLRKDDGEFWMTLQDFKAHFMLLVICKLTPGLLSQEVGQKWAYTMREGRWEKGTTAGGRMKSSRDTFWKNPQFLLSVWRPQEGKKLQMPCRVLVSLLQKPRHRHRNQKLHFAIGFYVFRDYEDQRRLPRGFFWKNAPVSWPKEFLTEKEVSQELWLEPGTYLIVPCTSEARQESEFVLRVLSRKHVFYEIGSNSSAVFSKETGDQNRGQDELFTKLFEKHPETNAAQRQKIPNHLTSSIADDDSTLLRRLEPCLPSLRTLHMAPEWSLADTNPCPTHLLFFRTMCPAITSSAGKISLLQISPLDGQCAKRKVKIALDLLRLALLLVATTLSQQRCNLTRPKQSDSRVGVLTSIQYSLHSYPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LT31</accession>
    <name>A0A8C4LT31_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable E3 ubiquitin-protein ligase HECTD2</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">HECT domain-containing protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">HECT-type E3 ubiquitin transferase HECTD2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">HECTD2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="RefSeq" id="XP_014694910.1">
        <property type="nucleotide sequence ID" value="XM_014839424.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LT31"/>
    <dbReference type="Ensembl" id="ENSEAST00005017301.2">
        <property type="protein sequence ID" value="ENSEASP00005015915.1"/>
        <property type="gene ID" value="ENSEASG00005011060.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106830035"/>
    <dbReference type="KEGG" id="eai:106830035"/>
    <dbReference type="CTD" id="143279"/>
    <dbReference type="GeneTree" id="ENSGT00940000157750"/>
    <dbReference type="OMA" id="DLMSEYY"/>
    <dbReference type="OrthoDB" id="5981550at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000009">
        <property type="entry name" value="Probable E3 ubiquitin-protein ligase HECTD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000023">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HECTD2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000004">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF9">
        <property type="entry name" value="HECT-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="13">
        <location>
            <begin position="437"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="11">
        <location>
            <position position="744"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00056288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00077386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00083730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005015915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="776" mass="88245" checksum="94854CEC4CEC8200" modified="2022-01-19" version="1">MSEEGRVPSPAALPAEATAAPEEKKGKEPERDKLPPIVSAGASASAGLDRGSKGQVSTFSSCVSAVSQKKEAAENRSSPAHLVFPNIKNVRDLPPTCLDVRQKQRISMDALSSEMKAPVLPEPVLPVQPKTMKDFQEDVEKVKSSGDWKAVHDFYLTTFDSFPELNAAFKKDASASFNTIEDSGINAKFVNAVYDTLLNTPQDIQKTVLKGIINSLLREWKGPRTKDDLRAYFILLQNPQFNNTSTYVIYAHLLRQIATLVEADHHFLVHWFKKLSQKRFKQLVERLLQFISLRLFPAKPEEFPPIAKCSWWIPSASKVLALLNTANDLVHPPLIPYIDFYNSTLDHIDLMEEYHTWQSFGNSHRFSFCQYPFVISIAAKKIIIQRDSEQQMISIARQSLVDKVSRRQRPDMNMLFLNMKVRRTHLVSDSLDELTRKRADLKKKLKVTFVGEAGLDMGGLTKEWFLLLIRQIFHPDYGMFTYHKDSYCHWFSSFKCDNYSEFRLVGILMGLAVYNSITLDIRFPPCCYKKLLSPPIVPSDQNTPVGICSVTTDDLCQIMPELAHGLSELLSYEGNVEEDFYSTFQVFQEEFGIIKSYNLKPGGDKIPVTNQNRKEYVQLYIDFLLNKSIYKQFAAFYYGFHSVCASNALMLLRPEEVEILVCGSPELDMHALQRSTQYDGYAKTDLTIRYFWDVVLGFPLDLQKKLLHFTTGSDRVPVGGMADLNFKISKNETSTNWLPIAHTCFNQLCLPPYKSKKDLKQKLIIGISNSEGFGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LT67</accession>
    <name>A0A8C4LT67_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">U5 small nuclear ribonucleoprotein 40 kDa protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">WD repeat-containing protein 57</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SNRNP40</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Required for pre-mRNA splicing as component of the activated spliceosome. Component of the U5 small nuclear ribonucleoprotein (snRNP) complex and the U4/U6-U5 tri-snRNP complex, building blocks of the spliceosome. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the pre-catalytic and catalytic spliceosome complexes. Component of the postcatalytic spliceosome P complex. Part of the U5 snRNP complex. Interacts with PRPF8. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, WDR57, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39. Component of the minor spliceosome, which splices U12-type introns.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014693451.1">
        <property type="nucleotide sequence ID" value="XM_014837965.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LT67"/>
    <dbReference type="SMR" id="A0A8C4LT67"/>
    <dbReference type="Ensembl" id="ENSEAST00005016866.2">
        <property type="protein sequence ID" value="ENSEASP00005015504.1"/>
        <property type="gene ID" value="ENSEASG00005010818.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106829073"/>
    <dbReference type="KEGG" id="eai:106829073"/>
    <dbReference type="CTD" id="9410"/>
    <dbReference type="GeneTree" id="ENSGT00940000155058"/>
    <dbReference type="OMA" id="IWDIRPY"/>
    <dbReference type="OrthoDB" id="1068471at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000375">
        <property type="term" value="P:RNA splicing, via transesterification reactions"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000229">
        <property type="entry name" value="Small nuclear ribonucleoprotein U5 subunit 40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052234">
        <property type="entry name" value="U5_snRNP_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006:SF1">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 11" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="63"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="106"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="148"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="190"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="232"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="293"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="11">
        <location>
            <begin position="324"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005015504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="358" mass="39382" checksum="CEAC7DBB0DB7E494" modified="2022-01-19" version="1">MIEQQKRKGPELPLVPVKRQRHELLLGATGSGPGAGQQQAAPGALLQAGPPRCSSLQAPIMLLSGHEGEVYCCKFHPNGSTLASAGFDRLILLWNVYGDCDNYATLKGHSGAVMELHYNTDGSMLFSASTDKTVAVWDSETGERVKRLKGHTSFVNSCYPARRGPQLVCTGSDDGTVKLWDIRKKAAIQTFQNTYQVLAVTFNDTSDQIISGGIDNDIKVWDLRQNKLTYTMRGHADSVTGLSLSSEGSYLLSNAMDNTVRVWDVRPFAPKERCVKIFQGNVHNFEKNLLRCSWSPDGSKIAAGSADRFVYVWDTTSRRILYKLPGHAGSINEVAFHPDEPIILSASSDKRLYMGEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LV15</accession>
    <name>A0A8C4LV15_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Schlafen family member 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SLFN5</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Schlafen family. Subgroup III subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014717703.1">
        <property type="nucleotide sequence ID" value="XM_014862217.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044601129.1">
        <property type="nucleotide sequence ID" value="XM_044745194.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LV15"/>
    <dbReference type="Ensembl" id="ENSEAST00005017533.2">
        <property type="protein sequence ID" value="ENSEASP00005016133.2"/>
        <property type="gene ID" value="ENSEASG00005011202.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106844643"/>
    <dbReference type="KEGG" id="eai:106844643"/>
    <dbReference type="CTD" id="162394"/>
    <dbReference type="GeneTree" id="ENSGT00410000025651"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001322">
        <property type="entry name" value="Schlafen family member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.950.30:FF:000001">
        <property type="entry name" value="Schlafen family member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.30">
        <property type="entry name" value="Schlafen, AAA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031450">
        <property type="entry name" value="Poxin-SLFN/SLFN_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029684">
        <property type="entry name" value="Schlafen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007421">
        <property type="entry name" value="Schlafen_AlbA_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038461">
        <property type="entry name" value="Schlafen_AlbA_2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018647">
        <property type="entry name" value="SLFN_3-like_DNA/RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048729">
        <property type="entry name" value="SLFN_GTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027785">
        <property type="entry name" value="UvrD-like_helicase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12155">
        <property type="entry name" value="SCHLAFEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12155:SF26">
        <property type="entry name" value="SCHLAFEN FAMILY MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17057">
        <property type="entry name" value="B3R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09848">
        <property type="entry name" value="SLFN-g3_helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04326">
        <property type="entry name" value="SLFN_AlbA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21026">
        <property type="entry name" value="SLFN_GTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13538">
        <property type="entry name" value="UvrD_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Poxin-Schlafen/Schlafen-like N-terminal" evidence="7">
        <location>
            <begin position="81"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Schlafen AlbA-2" evidence="4">
        <location>
            <begin position="192"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Schlafen GTPase-like" evidence="8">
        <location>
            <begin position="384"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="domain" description="Schlafen group 3-like DNA/RNA helicase" evidence="5">
        <location>
            <begin position="574"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="6">
        <location>
            <begin position="837"/>
            <end position="884"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF09848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF21026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005016133.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="889" mass="101168" checksum="9207CAE6DB7A7C67" modified="2023-09-13" version="2">MSLKIDLETNFAECVLNAGKVVLGNKQRSEMYPQLREKQNEIILHAICALLNSGGGVVKAEIENKEYNYEIHGVGLHLPSIFNGYLDEMQQGDLFLIFVKSWNAEASGVRLATLCSNLYHRYRTSTDVMSSHEALAFLREKTQTVMNINDSSLSPRNVGVGVQNEGNIKASAAALFARAHLQYLEKLNFTTSKHVEFKMFSTDVSRCFKESLPSCVSAFANTEGGFVFFGVHDETLQVIGSKKERMDLTALKASIDRCIKKLPVHHFCTRRHEIKYAVKFLEVHNQCALHGYVCAVKVEPFCCAVFAKIPSSWQVKNNCVRQLSTKEWVAWMTEADPDLSGFSEMVLALSLSSTTPCSRSVCIHKNLECLEEQQKRYFPVFPDRVSYIPDTLYKELISQHKGLRDLINREMRSVSRGVLIFSRSWAVDLGLQEKQGVICDALLLSQNNIPLLYTILGEWDACWKDYSMRLARSLKLKLVNRGGYTGKLCITPLVFLLNPDRTAKTLHGSDLQIYPESYNLTTIQHMETLLQSLVIVLLGFRSFLSEELGSEVLNLLTDKQYELLSKNLRKTRELFVHGLPGSGKTILALRIMEKVRNVFHCQPDDILYVCENQPLRKFVSEKNVCQAVTRKTFMKNNFENIQHIIIDEAQNFRTEDGDWYEKARTITQREKDCPGILWIFLDYFQTSHLSCNGLPPLLDQDPKEELTRVVRNADPIANYLQEVMQEVKEKPPPNIPAGCLEMVHEAQWAQGVPGNFENMECSDLEEMVVRVAEKCQFLLRSGYSPKDIAVLFSKASEVEKYKDKLLRAMRRRRMSQLDEESDLVVRIKDASDITGNHIVLDSVRRFSGLERNIVFGINPSAAESAVFYNLLLCLASRAKKHLYILKVSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M4N7</accession>
    <name>A0A8C4M4N7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106825809</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M4N7"/>
    <dbReference type="Ensembl" id="ENSEAST00005020239.2">
        <property type="protein sequence ID" value="ENSEASP00005018652.2"/>
        <property type="gene ID" value="ENSEASG00005012853.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160590"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097228">
        <property type="term" value="C:sperm principal piece"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000178">
        <property type="entry name" value="Calmodulin-2 A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46763">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46763:SF1">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="33"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="2">
        <location>
            <begin position="111"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005018652.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="181" mass="21030" checksum="26F8E4E8B82B8BC0" modified="2023-09-13" version="2">NPQSFNVSYKKDPEWHGAYSHLARSLPEIAVAEFHKKIKEAFEVFDHESNNTVDVREIGTIIRSLGCCPSEGELHDLIAEVEEEEPTGYIRYEKFLPVMTQVLLERRYRPIPEDILLRAFEVLDPAKRGFLSKEELIKYMTEEGEPFSQEEMEEMLSAAIDPESNSIHYKDYITMMVIDEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M5H7</accession>
    <name>A0A8C4M5H7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-binding cassette sub-family F member 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ABCF3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4M5H7"/>
    <dbReference type="Ensembl" id="ENSEAST00005022499.2">
        <property type="protein sequence ID" value="ENSEASP00005020726.2"/>
        <property type="gene ID" value="ENSEASG00005014186.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155604"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03221">
        <property type="entry name" value="ABCF_EF-3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000104">
        <property type="entry name" value="ATP-binding cassette sub-family F member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000688">
        <property type="entry name" value="ATP-binding cassette sub-family F member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032781">
        <property type="entry name" value="ABC_tran_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058770">
        <property type="entry name" value="PWI_ABCF3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF117">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12848">
        <property type="entry name" value="ABC_tran_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26051">
        <property type="entry name" value="PWI_ABCF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="200"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="514"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="151"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="151"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="183"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020726.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="731" mass="82364" checksum="AFDA5E3A86E0BBAB" modified="2023-09-13" version="2">MGRGRALAEVSRRLRGREPGWNMATCAEILRSEFPEIDGQVFDYVTGVLHSGSADFESVDDLVEAVGELLQEVSGDSKDDAGIRAVCQRMYNTLRLAEPQSQGNSQVLLDAPIQLSKITENYDSGAKLPGLLKREQFSTVNAKKLEKAEARLKAKQEKRSEKDTLKTSNPLVLEEASASQAGSRKESRLESSGKNKSYDVRIENFDVSFGDRVLLTGADVNLAWGRRYGLVGRNGLGKTTLLKMLATRSLRVPAHISLLHVEQEVAGDDTPALQSVLESDTVREDLLRRERELSAQIAAGRAEGSEAAQLAEIYAKLEEIEADKAPARASVILAGLGFTPKMQQQPTREFSGGWRMRLALARALFARPDLLLLDEPTNMLDVRAILWLENYLQTWPSTILVVSHDRNFLNAIATDIIHLHSQRLDGYRGDFETFIKSKQERLLNQQREYEAQQQYRQHIQVFIDRFRYNANRASQVQSKLKMLEKLPELKPVDKELEVVMKFPDGFEKFSPPILQLDEVDFYYDPKHVIFSRLSVSADLESRICVVGENGAGKSTMLKLLMGDLAPVRGIRHAHRNLKIGYFSQHHVEQLDLNVSAVELLARKFPGRPEEEYRHQLGRYGISGELAVRPVASLSGGQKSRVAFAQMTMPCPNFYILDEPTNHLDMETIEALGRALNNFRGGVILVSHDERFIRLVCRELWVCEKGGVTRVEGGFDQYRALLQEQFRREGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M997</accession>
    <name>A0A8C4M997_EQUAS</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LOC106825746</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M997"/>
    <dbReference type="Ensembl" id="ENSEAST00005024345.2">
        <property type="protein sequence ID" value="ENSEASP00005022434.1"/>
        <property type="gene ID" value="ENSEASG00005015335.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00910000147256"/>
    <dbReference type="OMA" id="RPECATD"/>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005022434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="143" mass="15144" checksum="20D70BA4A7A2E9B7" modified="2022-01-19" version="1">MSQTRACSLPKKAPRAGSAPRPGLGRSPVMLHRPRRSQLCPRAGADRARSAGAGRPECATDAAGAQDAVSAKPGCRRSCPDGKPRNRHFLDGAGPEAAGLLRRMPVARALRVMRLWSPGWGLPKGCLWGLQGGFLLDRSPSQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MCC6</accession>
    <name>A0A8C4MCC6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SH2B adapter protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Adapter protein with pleckstrin homology and Src homology 2 domains</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">SH2 and PH domain-containing adapter protein APS</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">SH2B2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="8">Homodimer. Interacts with KIT/c-KIT, SHC1, EPOR, PDGFR, VAV1 and VAV3. Interacts (via N-terminal region) with SHC1. Interacts (via the phosphorylated C-terminus) with GRB2. Interacts (via its SH2 domain) with EPOR, INSR and KIT. Interacts with GRB2 after B-cell antigen receptor stimulation. Interacts (via PH domain) with VAV3. Interacts with NTRK1, NTRK2 and NTRK3 (phosphorylated); after stimulation of the receptor by its extracellular ligand and subsequent autophosphorylation of the receptor. Binds INSR, GRB2, ASB6 and CAP. Insulin stimulation leads to dissociation of CAP. Binds CBS only when SH2B2/APS has become phosphorylated. INSR binding does not depend on the phosphorylation of SH2B2/APS.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the SH2B adapter family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MCC6"/>
    <dbReference type="Ensembl" id="ENSEAST00005026904.2">
        <property type="protein sequence ID" value="ENSEASP00005024793.2"/>
        <property type="gene ID" value="ENSEASG00005016877.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183191"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042169">
        <property type="term" value="F:SH2 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005068">
        <property type="term" value="F:transmembrane receptor protein tyrosine kinase adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050853">
        <property type="term" value="P:B cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001922">
        <property type="term" value="P:B-1 B cell homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050873">
        <property type="term" value="P:brown fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019221">
        <property type="term" value="P:cytokine-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01231">
        <property type="entry name" value="PH_SH2B_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10411">
        <property type="entry name" value="SH2_SH2B2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000008">
        <property type="entry name" value="SH2B adapter protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000187">
        <property type="entry name" value="SH2B adapter protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015012">
        <property type="entry name" value="Phe_ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036290">
        <property type="entry name" value="Phe_ZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030523">
        <property type="entry name" value="SH2B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035058">
        <property type="entry name" value="SH2B2_SH2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10872">
        <property type="entry name" value="SH2B ADAPTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10872:SF4">
        <property type="entry name" value="SH2B ADAPTER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08916">
        <property type="entry name" value="Phe_ZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00401">
        <property type="entry name" value="SH2DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109805">
        <property type="entry name" value="Phenylalanine zipper"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0727">SH2 domain</keyword>
    <feature type="domain" description="PH" evidence="15">
        <location>
            <begin position="183"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="14">
        <location>
            <begin position="405"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="368"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="503"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="386"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="507"/>
            <end position="523"/>
        </location>
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    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014688863.1">
        <property type="nucleotide sequence ID" value="XM_014833377.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044611471.1">
        <property type="nucleotide sequence ID" value="XM_044755536.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070344516.1">
        <property type="nucleotide sequence ID" value="XM_070488415.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070344523.1">
        <property type="nucleotide sequence ID" value="XM_070488422.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070344526.1">
        <property type="nucleotide sequence ID" value="XM_070488425.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070344530.1">
        <property type="nucleotide sequence ID" value="XM_070488429.1"/>
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    <dbReference type="RefSeq" id="XP_070344531.1">
        <property type="nucleotide sequence ID" value="XM_070488430.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070344548.1">
        <property type="nucleotide sequence ID" value="XM_070488447.1"/>
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    <dbReference type="RefSeq" id="XP_070344553.1">
        <property type="nucleotide sequence ID" value="XM_070488452.1"/>
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    <dbReference type="RefSeq" id="XP_070344558.1">
        <property type="nucleotide sequence ID" value="XM_070488457.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070344566.1">
        <property type="nucleotide sequence ID" value="XM_070488465.1"/>
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    <dbReference type="RefSeq" id="XP_070344572.1">
        <property type="nucleotide sequence ID" value="XM_070488471.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A8C4MFY3"/>
    <dbReference type="Ensembl" id="ENSEAST00005023876.2">
        <property type="protein sequence ID" value="ENSEASP00005022008.1"/>
        <property type="gene ID" value="ENSEASG00005015015.2"/>
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    <dbReference type="GeneID" id="106826167"/>
    <dbReference type="KEGG" id="eai:106826167"/>
    <dbReference type="CTD" id="9886"/>
    <dbReference type="GeneTree" id="ENSGT00940000159995"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
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    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="BACK_RHOBTB1"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd18355">
        <property type="entry name" value="BTB1_POZ_RHOBTB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18358">
        <property type="entry name" value="BTB2_POZ_RHOBTB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01873">
        <property type="entry name" value="RhoBTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000069">
        <property type="entry name" value="Rho related BTB domain containing 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000177">
        <property type="entry name" value="Rho-related BTB domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000014">
        <property type="entry name" value="Rho-related BTB domain-containing protein 2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003578">
        <property type="entry name" value="Small_GTPase_Rho"/>
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    <dbReference type="PANTHER" id="PTHR24072">
        <property type="entry name" value="RHO FAMILY GTPASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
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    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="2"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="7">
        <location>
            <begin position="266"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="7">
        <location>
            <begin position="487"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="306"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="310"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="339"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005022008.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="698" mass="79657" checksum="13E37C946014AF44" modified="2023-09-13" version="1">MDTDMDYERPNVETIKCVVVGDNAVGKTRLICARACNTTLTQYQLLATHVPTVWAIDQYRVCQEVLERSRDVVDEVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLNHVKTMWYQEIKHFCPRTPVVLVGCQLDLRYADLEAVNRARRPLARPIKRGDILPPEKGREVAKELGIPYYETSVFDQFGIKDVFDNAIRAALIARRHLQFWKSHLKKVQKPLLQAPFLPPKAPPPVIRVPECPSTGTNEAACLLDSPLCADVLFILQDQEHIFAHRIYLATSSSKFYDLFLMECEETPNGSKGACEKEKQSRDFEGQALSVDPDEEKEEGTPRTPQANQWKSSSQNLFQQETLGLEAEGSIPETQTLSGWSKGFVGMHKEMQVNPISKRVGPMTVVRMDSSVQPGPFRTLLQFLYTGQLDEKEKDLVGLAQIAEVLEMFDLRMMVENIMNKEAFMNQEITKAFHVRKANRIKECLSKGTFSDVTFKLDDGAISAHKPLLICSCKWMAAMFGGSFVESANSEVYLPNINKISMQAVLDYLYTKQLSPNLDLDPLELIALANRFCLPHLVALAEQHAVQELTKSAMNGVGIDGEVLSYLELAQFHNAHQLAAWCLHHICTNYNSVCSKFRKEIKSKSADNQEYFERHRWPPVWYLKEEDHYQRVKREREKEDIALNKHHSRRKWCFWNSSPAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MGI0</accession>
    <name>A0A8C4MGI0_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Calcium release activated channel regulator 2A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">CRACR2A</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSEASP00005025119.2"/>
        <property type="gene ID" value="ENSEASG00005017095.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831503"/>
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        <property type="component" value="Chromosome 22"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008104">
        <property type="term" value="P:intracellular protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051928">
        <property type="term" value="P:positive regulation of calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002115">
        <property type="term" value="P:store-operated calcium entry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001129">
        <property type="entry name" value="ras-related protein Rab-44 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Liu"/>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Heme-dependent dioxygenase that catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring and converts L-tryptophan to N-formyl-L-kynurenine. Catalyzes the oxidative cleavage of the indole moiety.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 10">
            <text>L-tryptophan + O2 = N-formyl-L-kynurenine</text>
            <dbReference type="Rhea" id="RHEA:24536"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58629"/>
            <dbReference type="EC" id="1.13.11.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
        <text evidence="10">Binds 1 heme group per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8 10">Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9 10">Homotetramer. Dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the tryptophan 2,3-dioxygenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.13.11.11" evidence="10"/>
    <dbReference type="RefSeq" id="XP_014699525.1">
        <property type="nucleotide sequence ID" value="XM_014844039.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MIW2"/>
    <dbReference type="Ensembl" id="ENSEAST00005025177.2">
        <property type="protein sequence ID" value="ENSEASP00005023200.1"/>
        <property type="gene ID" value="ENSEASG00005015804.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106833017"/>
    <dbReference type="KEGG" id="eai:106833017"/>
    <dbReference type="CTD" id="6999"/>
    <dbReference type="GeneTree" id="ENSGT00390000008593"/>
    <dbReference type="OMA" id="WRWRNDH"/>
    <dbReference type="OrthoDB" id="447477at2759"/>
    <dbReference type="UniPathway" id="UPA00333">
        <property type="reaction ID" value="UER00453"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004833">
        <property type="term" value="F:L-tryptophan 2,3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019442">
        <property type="term" value="P:L-tryptophan catabolic process to acetyl-CoA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019441">
        <property type="term" value="P:L-tryptophan catabolic process to kynurenine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051289">
        <property type="term" value="P:protein homotetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3810:FF:000001">
        <property type="entry name" value="Tryptophan 2,3-dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01972">
        <property type="entry name" value="T23O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037217">
        <property type="entry name" value="Trp/Indoleamine_2_3_dOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004981">
        <property type="entry name" value="Trp_2_3_dOase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10138">
        <property type="entry name" value="TRYPTOPHAN 2,3-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10138:SF0">
        <property type="entry name" value="TRYPTOPHAN 2,3-DIOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03301">
        <property type="entry name" value="Trp_dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140959">
        <property type="entry name" value="Indolic compounds 2,3-dioxygenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 10" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="1 10" id="KW-0349">Heme</keyword>
    <keyword evidence="5 10" id="KW-0408">Iron</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0823">Tryptophan catabolism</keyword>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="231"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005023200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="406" mass="47849" checksum="A5545B2A5C0DE05F" modified="2022-01-19" version="1">MSGCPFLGNRFGYTFKNLSVEGSEEDKSQTGVNRASKGGLIYGNYLHLEKVLSAQELQSEVKGNKIHDEHLFIITHQAYELWFKQILWELDSVREIFQNGHVRDERNMLKVVTRMHRVVVILKLLVQQFSVLETMTALDFNDFREYLSPASGFQSLQFRLLENKIGVLQSLRVPYNRRHYRDNFQGEDNELLLKSEQEKTLLQLLEAWLERTPGLEPHGFNFWEKLETNIVQGLQEEFTKLQAKEESEEKEEQMAEFQKQREVLLSLFDEKRHEHLLSKGDRRLSYRALQGALMIYFYREEPRFQVPFQLLTSLMDIDTLMTKWRYNHVCMVHRMLGSKAGTGGSSGYQYLRSTVSDRYKVFVDLFNLSTYLVPRHWIPKMNPIIHKFLYTAEYCDSSYFSSDESD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MIX6</accession>
    <name>A0A8C4MIX6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ADP-ribosylation factor-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ARL2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the small GTPase superfamily. Arf family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MIX6"/>
    <dbReference type="Ensembl" id="ENSEAST00005029016.2">
        <property type="protein sequence ID" value="ENSEASP00005026725.2"/>
        <property type="gene ID" value="ENSEASG00005018231.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157941"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04154">
        <property type="entry name" value="Arl2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001722">
        <property type="entry name" value="ADP-ribosylation factor-like protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045873">
        <property type="entry name" value="Arl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044612">
        <property type="entry name" value="ARL2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45697">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR-LIKE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0519">Myristate</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="23"/>
            <end position="30"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026725.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="157" mass="18018" checksum="3EBB55DC3EDDAF1B" modified="2023-09-13" version="2">MGLLTILKKMKQKERELRLLMLGLDNAGKTTILKKFNGEDIDTISPTLGFNIKTLEHRGFKLNIWDVGGQKSLRSYWRNYFESTDGLIWVVDSADRQRMQDCQRELQSLLVEEALELDSIRSHHWCIQGCSAVTGENLLSGIDWLLDDISSRIFTAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MK15</accession>
    <name>A0A8C4MK15_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Rhomboid-related protein 1</fullName>
            <ecNumber evidence="4 13">3.4.21.105</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">RHBDL1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11 13">May be involved in regulated intramembrane proteolysis and the subsequent release of functional polypeptides from their membrane anchors.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.</text>
            <dbReference type="EC" id="3.4.21.105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase S54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.105" evidence="4 13"/>
    <dbReference type="RefSeq" id="XP_014704370.1">
        <property type="nucleotide sequence ID" value="XM_014848884.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MK15"/>
    <dbReference type="Ensembl" id="ENSEAST00005029257.2">
        <property type="protein sequence ID" value="ENSEASP00005026948.2"/>
        <property type="gene ID" value="ENSEASG00005018362.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106836175"/>
    <dbReference type="KEGG" id="eai:106836175"/>
    <dbReference type="CTD" id="9028"/>
    <dbReference type="GeneTree" id="ENSGT00940000160651"/>
    <dbReference type="OrthoDB" id="418595at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1540.10:FF:000006">
        <property type="entry name" value="rhomboid-related protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022764">
        <property type="entry name" value="Peptidase_S54_rhomboid_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017213">
        <property type="entry name" value="Peptidase_S54_rhomboid_met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051739">
        <property type="entry name" value="Rhomboid_IM_Serine_Proteases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45840:SF4">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01694">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037470">
        <property type="entry name" value="Rhomboid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="187"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="312"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="343"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S54 rhomboid" evidence="16">
        <location>
            <begin position="183"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="14">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR037470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR037470-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026948.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="377" mass="42200" checksum="1B92CC81BB1372F7" modified="2023-09-13" version="2">MDRSSLLQLIQEQQLDPENTGFIGADTFTGLVHSHELPLDPAKLDMLVALAQSNEQGQVCYQELVDLVSATISSKRSSSFKRAIANGQRALPRDGLLDEPGLGVYKRFVRYVAYEILPREVDRHWYFYRHRSCPPPVFMASVTLAQIVVFLCYGARLNKWVLQTYHPEYMRSPLVYHPSHRARAWRFLTYMFMHVGLEQLGFNALLQLMIGVPLEMVHGLLRISLLYLAGVLAGSLTVSITDMRAPVVGGSGGVYALCSAHLANVVMNWAGMRCPYKLLRMVLALVCMSSEVGRAVWLRFSPPLPASGPQPSFMAHLAGAVVGVSMGLTILRSYEERLRDQCGWWVVLLAYGTFLLFAIFWNIFAYDLLGAHIPPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MKN8</accession>
    <name>A0A8C4MKN8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Long-wave-sensitive opsin 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Red cone photoreceptor pigment</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Red-sensitive opsin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC106825615</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Membrane</location>
            <topology evidence="1 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044620095.1">
        <property type="nucleotide sequence ID" value="XM_044764160.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MKN8"/>
    <dbReference type="Ensembl" id="ENSEAST00005030645.2">
        <property type="protein sequence ID" value="ENSEASP00005028218.2"/>
        <property type="gene ID" value="ENSEASG00005019183.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106825615"/>
    <dbReference type="KEGG" id="eai:106825615"/>
    <dbReference type="GeneTree" id="ENSGT01030000234549"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001750">
        <property type="term" value="C:photoreceptor outer segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000090">
        <property type="entry name" value="Long-wave-sensitive opsin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050125">
        <property type="entry name" value="GPCR_opsins"/>
    </dbReference>
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</entry>
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                <person name="Liu"/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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    <accession>A0A8C4MLE9</accession>
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                <person name="G."/>
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                <person name="J."/>
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                <person name="Y."/>
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                <person name="G."/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May participate in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Participates in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Double stacked ring-shaped homooctamer. Interacts with BRCA2. Interacts with the MND1-PSMC3IP heterodimer. Interacts with RAD51AP1; the interaction is direct and stimulates DMC1-mediated homologous recombination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the RecA family. DMC1 subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_044779709.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044635646.2">
        <property type="nucleotide sequence ID" value="XM_044779711.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MLE9"/>
    <dbReference type="SMR" id="A0A8C4MLE9"/>
    <dbReference type="Ensembl" id="ENSEAST00005029934.2">
        <property type="protein sequence ID" value="ENSEASP00005027559.2"/>
        <property type="gene ID" value="ENSEASG00005018765.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837651"/>
    <dbReference type="KEGG" id="eai:106837651"/>
    <dbReference type="CTD" id="11144"/>
    <dbReference type="GeneTree" id="ENSGT00760000119398"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0000800">
        <property type="term" value="C:lateral element"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000150">
        <property type="term" value="F:DNA strand exchange activity"/>
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        <property type="term" value="F:identical protein binding"/>
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        <property type="term" value="P:DNA recombinase assembly"/>
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        <property type="term" value="P:oocyte maturation"/>
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        <property type="term" value="P:ovarian follicle development"/>
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    <dbReference type="GO" id="GO:0007286">
        <property type="term" value="P:spermatid development"/>
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        <property type="entry name" value="Meiotic recombination protein DMC1"/>
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    </dbReference>
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        <property type="entry name" value="meiotic recombination protein DMC1/LIM15 homolog"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="AAA+_ATPase"/>
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        <property type="entry name" value="Dmc1"/>
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    <dbReference type="InterPro" id="IPR016467">
        <property type="entry name" value="DNA_recomb/repair_RecA-like"/>
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    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
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    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020587">
        <property type="entry name" value="RecA_monomer-monomer_interface"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003301">
        <property type="entry name" value="PRK04301.1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF30">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN DMC1_LIM15 HOMOLOG"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
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    <dbReference type="PIRSF" id="PIRSF005856">
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    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="SUPFAM" id="SSF47794">
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        <property type="entry name" value="RECA_3"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 11" id="KW-0469">Meiosis</keyword>
    <keyword evidence="3 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="97"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="RecA family profile 2" evidence="14">
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            <begin position="277"/>
            <end position="340"/>
        </location>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MQ93</accession>
    <name>A0A8C4MQ93_EQUAS</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Laurasiatheria</taxon>
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            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSEASG00005019923.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 30"/>
    </dbReference>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005029304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="204" mass="21224" checksum="3A7C5B29C721276D" modified="2022-01-19" version="1">VLRLGERLGHQRSLAAFGPRLAQALGRLGHIVAPLGLIELQLLLRVREEGEVGERLLLAQVGGGRPAGDSAVHAAGLEDLQLRRAGLGHAAAAARRLGALGQHASAVRRRLPARAPGLVRALAAAAAAAAQPHLGLRALDAAAQHSAREERMDNPRVCRHGGRGAQGGGAAKRCVNAGNQEAWHRSLACQSSSGLQSNLKRTFS</sequence>
</entry>
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    <accession>A0A8C4MQT1</accession>
    <name>A0A8C4MQT1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Delta(14)-sterol reductase TM7SF2</fullName>
            <shortName evidence="20">Delta-14-SR</shortName>
            <ecNumber evidence="20">1.3.1.70</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">3-beta-hydroxysterol Delta (14)-reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">C-14 sterol reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Sterol C14-reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Transmembrane 7 superfamily member 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">TM7SF2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Catalyzes the reduction of the C14-unsaturated bond of lanosterol, as part of the metabolic pathway leading to cholesterol biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 20">
            <text>4,4-dimethyl-5alpha-cholesta-8,24-dien-3beta-ol + NADP(+) = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18561"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17813"/>
            <dbReference type="ChEBI" id="CHEBI:18364"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.3.1.70"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>4,4-dimethyl-8,14-cholestadien-3beta-ol + NADPH + H(+) = 4,4-dimethyl-5alpha-cholest-8-en-3beta-ol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:46812"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78904"/>
            <dbReference type="ChEBI" id="CHEBI:87044"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>5alpha-cholest-8,14-dien-3beta-ol + NADPH + H(+) = 5alpha-cholest-8-en-3beta-ol + NADP(+)</text>
            <dbReference type="Rhea" id="RHEA:46456"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16608"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:86131"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 20">Steroid biosynthesis; cholesterol biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Endoplasmic reticulum membrane</location>
            <topology evidence="20">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="20">Microsome membrane</location>
            <topology evidence="20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the ERG4/ERG24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.70" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4MQT1"/>
    <dbReference type="Ensembl" id="ENSEAST00005028990.2">
        <property type="protein sequence ID" value="ENSEASP00005026699.2"/>
        <property type="gene ID" value="ENSEASG00005018217.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000417"/>
    <dbReference type="UniPathway" id="UPA00063"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050613">
        <property type="term" value="F:Delta14-sterol reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1630:FF:000001">
        <property type="entry name" value="delta(14)-sterol reductase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1630">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001171">
        <property type="entry name" value="ERG24_DHCR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018083">
        <property type="entry name" value="Sterol_reductase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21257:SF52">
        <property type="entry name" value="DELTA(14)-STEROL REDUCTASE TM7SF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01222">
        <property type="entry name" value="ERG4_ERG24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01017">
        <property type="entry name" value="STEROL_REDUCT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01018">
        <property type="entry name" value="STEROL_REDUCT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 20" id="KW-0152">Cholesterol biosynthesis</keyword>
    <keyword evidence="5 20" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="8 20" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 20" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13 20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 20" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="16 20" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="12 20" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="15 20" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="315"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="346"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="416"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00049367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU369120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026699.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="479" mass="52617" checksum="D55E5510A46F278D" modified="2023-09-13" version="2">MARGRGRLKILGRGWSLSELSAETGADAARSDPPRHRAHLLGVGVGCVFLTGFCELPTPSGAKTMASPQGSLEFGGPLGAAALMLVLPATMFHLLLVARSGPARLLGPPPYLPGPEALWSPRALQLVLAWLGLQAALYLLPARKVAEGQELKNKSRLRYPINGFQALVVTTLLVGLGVSAGLPLDALPEMLLPLAFAATLTAFVFSLLLYLKALVAPASALAPGGNSGNPIYDFFLGRELNPRISSFDFKYFCELRPGLMAWVLINLALLLKEAELRGSPSLAMWLVNGFQLLYVADALWHEEAVLTTMDITHDGFGFMLAFGDLAWVPFTYSLQAQFLLYHPQPLGLPMASVICLINAIGYYVFRGANSQKNTFRKNPSDPRVADLETIPTATGRQLLVSGWWGMVRHPNYLGDLIMALAWSLPCGVSHLLPYFYLLYFTALLVHREARDEQQCLQKYGLAWREYCKRVPYRILPYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MQT2</accession>
    <name>A0A8C4MQT2_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">5'-nucleotidase domain containing 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">NT5DC3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5'(3')-deoxyribonucleotidase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MQT2"/>
    <dbReference type="Ensembl" id="ENSEAST00005033188.2">
        <property type="protein sequence ID" value="ENSEASP00005030529.2"/>
        <property type="gene ID" value="ENSEASG00005020426.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158637"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07522">
        <property type="entry name" value="HAD_cN-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000026">
        <property type="entry name" value="NT5DC3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008380">
        <property type="entry name" value="HAD-SF_hydro_IG_5-nucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016695">
        <property type="entry name" value="Pur_nucleotidase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02244">
        <property type="entry name" value="HAD-IG-Ncltidse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103">
        <property type="entry name" value="5'-NUCLEOTIDASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12103:SF11">
        <property type="entry name" value="5'-NUCLEOTIDASE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05761">
        <property type="entry name" value="5_nucleotid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017434">
        <property type="entry name" value="Purine_5'-nucleotidase"/>
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        <property type="entry name" value="HAD-like"/>
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    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="102"/>
        </location>
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    <feature type="binding site" evidence="6">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
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    <feature type="binding site" evidence="6">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
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        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009589"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR017434-1"/>
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    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR017434-2"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030529.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="567" mass="65846" checksum="1961E950F6D320EC" modified="2023-09-13" version="2">MATAAVAVARGAGARGAAATLRGGCGTAARGRPRAGPSRPLCTAPGTVPDRKRYLWERYQEAKRSTDELVPSIMNNLLNPDAIFANNEMSLSDIEIYGFDYDYTLVFYSKHLHTLIFNAARDLLINEHRYPVEIRKYEYDPSFAIRGLHYDVQRAVLMKIDAFHYIQLGTVYRGLSVVPDEEVIEMYEGSHVPLEQMSDFYGKSSHGNTMKQFMDIFSLPEMTLLSCVNEYFLKNNIDYEPVHLYKDVKDSIRDVHIKGIMYRAIEADIEKYICYAEQTRAVLAKLADHGKKMFLITNSPSSFVDKGMRYIVGKDWRDLFDVVIVQAEKPNFFNDKRRPFRKVNEKGVLLWDKIHKLQKGQIYKQGNLYEFLKLTGWRGSKVLYFGDHIYSDLADLTLKHGWRTGAIIPELRSELRIMNTEQYVQTMTWLQTLTGLLEQMQRCAESLLYTQHSFNSHSCPVQVHRDAESQLVLQEWKKERKEMREMTKSFFNAQFGSLFRTDQNPTYFLRRLSRFADIYMASLSCLLNYDVNHTFYPRRTPLQHELPAWSERPPTFRLPLLQEAQAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MRS1</accession>
    <name>A0A8C4MRS1_EQUAS</name>
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        <recommendedName>
            <fullName evidence="7">Epsilon-sarcoglycan</fullName>
        </recommendedName>
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        <name evidence="21" type="primary">SGCE</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <location evidence="4">Sarcolemma</location>
            <topology evidence="4">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the sarcoglycan alpha/epsilon family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044623083.1">
        <property type="nucleotide sequence ID" value="XM_044767148.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MRS1"/>
    <dbReference type="Ensembl" id="ENSEAST00005033236.2">
        <property type="protein sequence ID" value="ENSEASP00005030575.2"/>
        <property type="gene ID" value="ENSEASG00005020815.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106838931"/>
    <dbReference type="CTD" id="8910"/>
    <dbReference type="GeneTree" id="ENSGT00390000005672"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016012">
        <property type="term" value="C:sarcoglycan complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006644">
        <property type="entry name" value="Cadg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008908">
        <property type="entry name" value="Sarcoglycan_alpha/epsilon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048347">
        <property type="entry name" value="Sarcoglycan_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048346">
        <property type="entry name" value="Sarcoglycan_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10132">
        <property type="entry name" value="ALPHA-/EPSILON-SARCOGLYCAN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10132:SF17">
        <property type="entry name" value="EPSILON-SARCOGLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05510">
        <property type="entry name" value="Sarcoglycan_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20989">
        <property type="entry name" value="Sarcoglycan_2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00736">
        <property type="entry name" value="CADG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="317"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Dystroglycan-type cadherin-like" evidence="20">
        <location>
            <begin position="49"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="418"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004513"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00019887"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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            <dbReference type="SMART" id="SM00736"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030575.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="437" mass="49883" checksum="3329011C195CA826" modified="2023-09-13" version="2">MQLPRWWEPGDPCAWTGQGRGTRRMSPATTGTFLLTVYTIFSKVHSDRNVYPSAGVLFVHVLEREYFKGEFPPYPKPGEISNDPITFNTNLMGYPDRPGWLRYIQRTPYSDGVLYGSPTAENVGKPTIIEITAYNRRTFETARHNLIINIMSAEDFPLPYQAEFFIKNMNVEEMLASEVLGDFLGAVKNVWQPERLNAINITSALDRGGRVPLPINDMKEGVYVMVGADVPFSSCLREVENPQNQLRCSQEMEPVITCDKKFRTQFYIDWCKISLVDKTKQVSTYQEVIRGEGILPDGGEYKPPSDSLKSRDYYTDFLITLAVPSAVALVLFLILAYIMCCRREGVEKRNMQTPDIQLVHHSAIQKSTKELRDMSKNREIAWPLSTLPVFHPVTGELIRPLHADSYESTNMPLMQTQQNLPHQTQIPQQQTTGKWYP</sequence>
</entry>
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    <accession>A0A8C4MTB3</accession>
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            <fullName evidence="13">Vomeronasal type-1 receptor</fullName>
        </recommendedName>
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        <name evidence="16" type="primary">LOC106822655</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative pheromone receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MTB3"/>
    <dbReference type="Ensembl" id="ENSEAST00005034750.2">
        <property type="protein sequence ID" value="ENSEASP00005031909.1"/>
        <property type="gene ID" value="ENSEASG00005021784.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822655"/>
    <dbReference type="KEGG" id="eai:106822655"/>
    <dbReference type="GeneTree" id="ENSGT00960000186612"/>
    <dbReference type="OMA" id="QICIGIF"/>
    <dbReference type="OrthoDB" id="9606139at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016503">
        <property type="term" value="F:pheromone receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019236">
        <property type="term" value="P:response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007606">
        <property type="term" value="P:sensory perception of chemical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13949">
        <property type="entry name" value="7tm_V1R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000033">
        <property type="entry name" value="Vomeronasal type-1 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004072">
        <property type="entry name" value="Vmron_rcpt_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24062">
        <property type="entry name" value="VOMERONASAL TYPE-1 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03402">
        <property type="entry name" value="V1R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01534">
        <property type="entry name" value="VOMERONASL1R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="34"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="73"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="152"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="208"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="263"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="50"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005031909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="341" mass="38437" checksum="2034F66F5F79668B" modified="2022-01-19" version="1">MIHEAHASYSHAGSSGSEDNSRCLETGRMASRDLVIGMLFLIQTVVGILGNFSLLYHHLFLYFTGCRLRSKDLILNHMIVANSLTLLCKGVPQTMAAFGWNHFPSDLGCKLLFYLHRVGRGVTLGTTCLLSIFQAITISSRNSRWAELKVKAVSVIVISLFLCWILQMLINVMYAVFVSSNWSKKNITNQKHFGYCSAVRHDKTRDSLYAALLSVPDIFCFGLMLWASGLMVFILYRHKQRVKHIRRTHISPRSSPESRATQGILVLVSTFVSFYSLSSIFQICIGIFDNPNWLLLNISAWINGCFATACPFILMNRDSSMPRLCFAPLRISKPPKLITNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MV10</accession>
    <name>A0A8C4MV10_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Thimet oligopeptidase</fullName>
            <ecNumber evidence="13">3.4.24.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">THOP1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation. Able to degrade the amyloid-beta precursor protein and generate amyloidogenic fragments. Also acts as a regulator of cannabinoid signaling pathway by mediating degradation of hemopressin, an antagonist peptide of the cannabinoid receptor CNR1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Preferential cleavage of bonds with hydrophobic residues at P1, P2 and P3' and a small residue at P1' in substrates of 5 to 15 residues.</text>
            <dbReference type="EC" id="3.4.24.15"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="16">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the peptidase M3 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.15" evidence="13"/>
    <dbReference type="RefSeq" id="XP_014701113.1">
        <property type="nucleotide sequence ID" value="XM_014845627.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MV10"/>
    <dbReference type="Ensembl" id="ENSEAST00005035212.2">
        <property type="protein sequence ID" value="ENSEASP00005032333.2"/>
        <property type="gene ID" value="ENSEASG00005022042.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106834154"/>
    <dbReference type="KEGG" id="eai:106834154"/>
    <dbReference type="CTD" id="7064"/>
    <dbReference type="GeneTree" id="ENSGT00950000183171"/>
    <dbReference type="OrthoDB" id="534666at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006518">
        <property type="term" value="P:peptide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06455">
        <property type="entry name" value="M3A_TOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1370.10:FF:000016">
        <property type="entry name" value="Thimet oligopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.40:FF:000001">
        <property type="entry name" value="Thimet oligopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000006">
        <property type="entry name" value="Thimet oligopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.40">
        <property type="entry name" value="Endopeptidase. Chain P, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1370.10">
        <property type="entry name" value="Neurolysin, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024077">
        <property type="entry name" value="Neurolysin/TOP_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024080">
        <property type="entry name" value="Neurolysin/TOP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045090">
        <property type="entry name" value="Pept_M3A_M3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001567">
        <property type="entry name" value="Pept_M3A_M3B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804">
        <property type="entry name" value="PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11804:SF50">
        <property type="entry name" value="THIMET OLIGOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01432">
        <property type="entry name" value="Peptidase_M3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 16" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6 16" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M3A/M3B catalytic" evidence="17">
        <location>
            <begin position="227"/>
            <end position="675"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00039079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00045978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005032333.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="687" mass="77680" checksum="1B11CA4124E4FC80" modified="2023-09-13" version="2">MKPPAACAGDGLATAAACSVVNYLRWDLSAQQIGERTAELIEQTKRVYDQVGAQAFEDVSYESTLKALADVEVSYTVQRNILDFPQHVSPSKAIRTASTEADKKLSEFDVEMSMRQDVYQRIVWLQEKVQKDSLRPEASRYLERLIKLGRRNGLHLPAETQEKIKSIKKKLSLLCIDFNKNLNEDTTFLPFTRDELGGLPEDFLNSLEKAGGEKLKVTLKYPHYFPLLKKCHVPETRRKVEAAFNCRCKEENCAILRELVALRAQKSSLLGFSTHADYVLEMNMAKTSQVVATFLDELAQKLKPLGEQERAVILELKKAECEQRGLEFDGQINAWDMRYYMNQVEETRYSVDQNLLKEYFPMQVVTRGLLGIYQELLGLTFDLEESATVWHEDVKLYSVRDVGSGQVIGKFYLDLYPREGKYGHAACFGLQPGCLREDGSRQIAIAAMVANFTKPTPDAPSLLQHDEVETYFHEFGHVMHQLCSQAEFAMFSGTHVERDFVEAPSQMLENWVWEKEPLLRMSQHYRTGSAIPPELLEKLIKSRQANTGLFNLRQIVLAKVDQALHTQTAADPAEEYARLCQEILGVPATPGTNMPATFGHLAGGYDAQYYGYLWSEVYSMDMFHTRFKQEGVLNGKVGMDYRSCILRPGGSQDASAMLKLFLGRDPKQDAFLLSKGLQVEGSESPAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MVM6</accession>
    <name>A0A8C4MVM6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Integrin alpha-4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">CD49 antigen-like family member D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Integrin alpha-IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">VLA-4 subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">ITGA4</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="16">Heterodimer of an alpha and a beta subunit. The alpha subunit can sometimes be cleaved into two non-covalently associated fragments. Alpha-4 associates with either beta-1 or beta-7. Alpha-4 interacts with PXN, LPXN, and TGFB1I1/HIC5. Interacts with CSPG4 through CSPG4 chondroitin sulfate glycosaminoglycan. Interacts with JAML; integrin alpha-4/beta-1 may regulate leukocyte to endothelial cells adhesion by controlling JAML homodimerization. ITGA4:ITGB1 is found in a ternary complex with CX3CR1 and CX3CL1. Interacts with MDK. ITGA4:ITGB1 interacts with MDK; this interaction mediates MDK-induced osteoblast cells migration through PXN phosphorylation. Integrin ITGA4:ITGB1 interacts with SVEP1 (via Sushi domain 21); thereby inhibits Ca(2+) intracellular signaling and as a result represses vasocontraction. ITGA4:ITGB1 interacts with SELP. ITGA4:ITGB1 interacts with BCAM.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 22">Membrane</location>
            <topology evidence="1 22">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 22">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MVM6"/>
    <dbReference type="Ensembl" id="ENSEAST00005035545.2">
        <property type="protein sequence ID" value="ENSEASP00005032624.2"/>
        <property type="gene ID" value="ENSEASG00005022220.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106826601"/>
    <dbReference type="KEGG" id="eai:106826601"/>
    <dbReference type="CTD" id="3676"/>
    <dbReference type="GeneTree" id="ENSGT00940000158443"/>
    <dbReference type="OrthoDB" id="5317514at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034668">
        <property type="term" value="C:integrin alpha4-beta1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034669">
        <property type="term" value="C:integrin alpha4-beta7 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019960">
        <property type="term" value="F:C-X3-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033631">
        <property type="term" value="P:cell-cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003366">
        <property type="term" value="P:cell-matrix adhesion involved in ameboidal cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904646">
        <property type="term" value="P:cellular response to amyloid-beta"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035987">
        <property type="term" value="P:endodermal cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034113">
        <property type="term" value="P:heterotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050901">
        <property type="term" value="P:leukocyte tethering or rolling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045906">
        <property type="term" value="P:negative regulation of vasoconstriction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000353">
        <property type="term" value="P:positive regulation of endothelial cell apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905564">
        <property type="term" value="P:positive regulation of vascular endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034446">
        <property type="term" value="P:substrate adhesion-dependent cell spreading"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1460:FF:000009">
        <property type="entry name" value="Integrin alpha 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1510:FF:000019">
        <property type="entry name" value="Integrin subunit alpha 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.130:FF:000006">
        <property type="entry name" value="Integrin, alpha 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.930">
        <property type="entry name" value="Bicelle-embedded integrin alpha(iib) transmembrane segment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.130">
        <property type="entry name" value="Integrin alpha, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1460">
        <property type="entry name" value="Integrin domains. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1510">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1530">
        <property type="entry name" value="ntegrin, alpha v. Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013517">
        <property type="entry name" value="FG-GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013519">
        <property type="entry name" value="Int_alpha_beta-p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000413">
        <property type="entry name" value="Integrin_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018184">
        <property type="entry name" value="Integrin_alpha_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048285">
        <property type="entry name" value="Integrin_alpha_Ig-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048286">
        <property type="entry name" value="Integrin_alpha_Ig-like_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028994">
        <property type="entry name" value="Integrin_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032695">
        <property type="entry name" value="Integrin_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220">
        <property type="entry name" value="INTEGRIN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23220:SF78">
        <property type="entry name" value="INTEGRIN ALPHA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01839">
        <property type="entry name" value="FG-GAP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08441">
        <property type="entry name" value="Integrin_A_Ig_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20805">
        <property type="entry name" value="Integrin_A_Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20806">
        <property type="entry name" value="Integrin_A_Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00357">
        <property type="entry name" value="Integrin_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01185">
        <property type="entry name" value="INTEGRINA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00191">
        <property type="entry name" value="Int_alpha"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69318">
        <property type="entry name" value="Integrin alpha N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69179">
        <property type="entry name" value="Integrin domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51470">
        <property type="entry name" value="FG_GAP"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00242">
        <property type="entry name" value="INTEGRIN_ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 22" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 22" id="KW-0401">Integrin</keyword>
    <keyword evidence="12 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14 22" id="KW-0675">Receptor</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 22" id="KW-0732">Signal</keyword>
    <keyword evidence="4 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 22" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040532252" description="Integrin alpha-4" evidence="22">
        <location>
            <begin position="34"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="976"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="21">
        <location>
            <begin position="35"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="21">
        <location>
            <begin position="292"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="21">
        <location>
            <begin position="355"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="repeat" description="FG-GAP" evidence="21">
        <location>
            <begin position="416"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha first immunoglubulin-like" evidence="23">
        <location>
            <begin position="463"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha second immunoglobulin-like" evidence="24">
        <location>
            <begin position="622"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="Integrin alpha third immunoglobulin-like" evidence="25">
        <location>
            <begin position="777"/>
            <end position="949"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00064081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00068406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00079426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00080509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00081680"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU00803"/>
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    </evidence>
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            <dbReference type="RuleBase" id="RU003762"/>
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    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF08441"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF20805"/>
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    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF20806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005032624.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1033" mass="114546" checksum="95C9074DC7DBC422" modified="2023-09-13" version="2">MAGEARRGSGPRGAAVREAVMLLLCLGVPTGRPYNLDTESALLYEGPPNTLFGYSVVLHSHGANRWLVVGAPTANWLANASVVNPGAIYRCRIGKNPDRTCEQLQLGSPSGEPCGKTCLEERDNQWLGVTLSRQPGENGSIVTCGHRWKNIFYIKNENKLPTGVCYGMPSDLRAELSKRIAPCYQDFVKKFGENFASCQAGISSFYTEDLIVMGAPGSSYWTGSLFVYNITTNKYKAFLDKENQVKFGSYLGYSVGAGHFRSQHTTEVVGGAPQHEQIGKAYIFSIDAKELNILHEMKGKKLGSYFGASVCAVDLNADGFSDLLVGAPMQSVIREEGRVFVYINSGSGAVMNEMETELTGSDKYAARFGESIVNLGDIDNDGFEDIAIGAPQEDDLRGAIYIYNGRAEGISSAFSQRIEGLQISKSLSMFGQSISGQIDADNNGYVDVAVGAFRSDSAVVLRTRPVVIVEASLNHPESVNRTNFDCIENGLPSVCMDLKLCFSYKGKEVPGYIVLFYNMSLDVNRKAESPSRFYFSSNGTSDVITGSMKVSSRAANCRTHQAFMRKDVRDILTPIQIKAAYHLGHHVISKRSTEEFPPLQPILQQKREKDVIEKTINFARFCAHENCSADLQLSAKIGFLKPHENKTYLAVGNMKTLMLNVSLFNAGDDAYETTLHIKLPTGLYFIKILDLEEKQINCEVTDSSGIVKLDCNVGYIYVDHLSRIDVSFLLDASSLSRAEEDLNITVHAACENEGEMDDLKSNKVTLAIPLKYEVMLAVHGFVSPTSFVYGSVEENEPGTCMAEKMNLTFHVINTGYSMAPNVSVEIMVPNSFIPQTNKLFNILDVQTTTGECHFENYESNCVSEQQKGALETLKDIFRFLSKTDKRLLYCMKADPNCLNFLCNFGKMESGKEASVHVQLEGRPSILEMDETSALKFEIRATAFPEPNPRVIELNKDENVAHVLLEGLHHQRPKRHFTIAIISSSLLLGLILLLFISYVMWKVGFFKRQYKSILQEENRRDSWSYVNSKSNDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MWX7</accession>
    <name>A0A8C4MWX7_EQUAS</name>
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        <recommendedName>
            <fullName evidence="10">Multiple epidermal growth factor-like domains protein 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Epidermal growth factor-like protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">MEGF9</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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    <dbReference type="RefSeq" id="XP_044634873.1">
        <property type="nucleotide sequence ID" value="XM_044778938.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MWX7"/>
    <dbReference type="Ensembl" id="ENSEAST00005035186.2">
        <property type="protein sequence ID" value="ENSEASP00005032308.2"/>
        <property type="gene ID" value="ENSEASG00005022032.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106824183"/>
    <dbReference type="KEGG" id="eai:106824183"/>
    <dbReference type="CTD" id="1955"/>
    <dbReference type="GeneTree" id="ENSGT00940000161177"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009887">
        <property type="term" value="P:animal organ morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009888">
        <property type="term" value="P:tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000188">
        <property type="entry name" value="Laminin subunit gamma 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000720">
        <property type="entry name" value="Multiple EGF like domains 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000589">
        <property type="entry name" value="Multiple epidermal growth factor-like domains 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000599">
        <property type="entry name" value="multiple epidermal growth factor-like domains protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000743">
        <property type="entry name" value="Si:dkey-220k22.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050440">
        <property type="entry name" value="Laminin/Netrin_ECM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574:SF406">
        <property type="entry name" value="LAMININ SUBUNIT ALPHA 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10574">
        <property type="entry name" value="NETRIN/LAMININ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 12" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="6 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 15" id="KW-0732">Signal</keyword>
    <keyword evidence="2 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040258370" description="Multiple epidermal growth factor-like domains protein 9" evidence="15">
        <location>
            <begin position="36"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="511"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="200"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="250"/>
            <end position="296"/>
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    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="297"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="345"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="16">
        <location>
            <begin position="396"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="35"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="79"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="182"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="250"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="316"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
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            <begin position="328"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="370"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="421"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="ARBA" id="ARBA00070404"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
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            <dbReference type="ARBA" id="ARBA00078818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005032308.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="598" mass="62559" checksum="B073B1131A8572DD" modified="2023-09-13" version="2">MNGGAERAMRSLPSLGGLALLCCAAAAAAASAASAGNVTGGGGAAGQVDASPGPGLGGEPSHPFSKATAPTTPAPRTGPPRATVHRPLAASSSAPSPETTPPRATAGPAPTTLWTTAGPAPTTPPAVERLPTTPRAPTRPAPTTLSTTTGPAPTTPVATTDLAPSPPLTPTRLPPGRRASVPPAPPVTEAPSSPPPEYICNCSVVGSLDVNRCNQTTGQCECHPDYQGLRCETCKESFYLNRTSGLCLPCDCSPHGSLSMSCNSSGKCQCKLGVTGSTCDRCQDGYYDFSKNGCLPCQCNNRSASCDALTGACLNCQENSKGDHCEECKEGFYQSPDVTKGCLRCPCSAVTSTGSCIINSGELEPTCVQCKDGYTGQNCNKCENGYYNSDSICTKCQCHGHVDPIKTPKICKPESGECINCLHNTTGFWCENCLEGYVRDREGNCIKKEVIVPTPEGSTILVSNASLTTSVPTPVINSTFTPTTLQTIFSVSSAENSTSALADVSWTQFNIIILTVIIIVVVLLMGFVGAVYMYREYQNRKLNAPFWTIELKEDNISFSSYHDSIPNADVSGLLEDDGNEVAPNGQLTLTTPMHNYKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MXJ7</accession>
    <name>A0A8C4MXJ7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="24 25">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3'-AMP(in) + ADP(out) + H(+)(out) = 3'-AMP(out) + ADP(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73679"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:60880"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>3'-AMP(out) + phosphate(in) = 3'-AMP(in) + phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:73691"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60880"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ADP(out) + diphosphate(in) = ADP(in) + diphosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:73671"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ADP(out) + phosphate(in) + H(+)(out) = ADP(in) + phosphate(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:65844"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>AMP(out) + phosphate(in) = AMP(in) + phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:70259"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>Mg(2+)(in) + ADP(out) + ATP(in) + H(+)(out) = Mg(2+)(out) + ADP(in) + ATP(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73659"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>dADP(in) + ADP(out) = dADP(out) + ADP(in)</text>
            <dbReference type="Rhea" id="RHEA:72855"/>
            <dbReference type="ChEBI" id="CHEBI:57667"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>dADP(out) + phosphate(in) + H(+)(out) = dADP(in) + phosphate(out) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73695"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57667"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>dAMP(in) + ADP(out) + H(+)(out) = dAMP(out) + ADP(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73675"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58245"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>dAMP(out) + phosphate(in) = dAMP(in) + phosphate(out)</text>
            <dbReference type="Rhea" id="RHEA:73687"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58245"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>phosphate(in) + ATP(out) + 2 H(+)(out) = phosphate(out) + ATP(in) + 2 H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:72035"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 22">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MXJ7"/>
    <dbReference type="Ensembl" id="ENSEAST00005036057.2">
        <property type="protein sequence ID" value="ENSEASP00005033082.2"/>
        <property type="gene ID" value="ENSEASG00005022628.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164117"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033391">
        <property type="term" value="C:chromatoid body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000028">
        <property type="entry name" value="Putative calcium-binding mitochondrial carrier protein scamc-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24089">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="9 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 21 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 22" id="KW-0813">Transport</keyword>
    <feature type="domain" description="EF-hand" evidence="23">
        <location>
            <begin position="19"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="23">
        <location>
            <begin position="86"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="23">
        <location>
            <begin position="122"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="21">
        <location>
            <begin position="192"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="21">
        <location>
            <begin position="276"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00036908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00049234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005033082.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="439" mass="49213" checksum="D2CB82F79997B8D2" modified="2023-09-13" version="2">MLRWLRGFVPPEAACQEDDDSFRYESLFRDLDRHEDGVVDIVELQEGLKNWIPAGPRADALHVFKAGDTNNDSRLDFEEFVQYLQDHEKKMRLAFNSVDKNNDGVIETSEVIAALKSLGMNISESQAKKILQSIDSDGTMTVDWDEWKYYFLFHPATTINEIARFWKHSTVRLLWESIAIPDEFTAQEKCSGDWWRPLVAGGIAGAVSRMYTAPLDCLKVMMQVHSLKSRRMRLTGGFEQMVKEGGLRSLWRGNGVNIFKIAPETALKIGAYEQYKKWLSFDGAKIGAGVTTQTCIYPMEVLKTRLAVGKTGQYSGVIDCGKKLLKQEGVRTFYKGYIPNLPGIISCAGIHLAVYEWLENSAGDSVDPGIVILLGCSMLAHTCGQIASFPLNLIRTRIQAQGEVLRFFRGLTPNIVKVLPAVGFSCVAYEKVKQHLGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MXL4</accession>
    <name>A0A8C4MXL4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">KH RNA binding domain containing, signal transduction associated 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">KHDRBS3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KHDRBS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044637083.1">
        <property type="nucleotide sequence ID" value="XM_044781148.2"/>
    </dbReference>
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        <property type="term" value="F:identical protein binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
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        <property type="entry name" value="KH-I_KHDRBS3"/>
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    <keyword evidence="5" id="KW-0729">SH3-binding</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="54"/>
            <end position="152"/>
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            <begin position="195"/>
            <end position="268"/>
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            <begin position="253"/>
            <end position="262"/>
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        <source>
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            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="346" mass="38804" checksum="EC17EECE8B3751ED" modified="2023-09-13" version="2">MEEKYLPELMAEKDSLDPSFTHALRLVNQEIEKFQKGEGKDEEKYIDVVINKNMKLGQKVLIPVKQFPKFNFVGKLLGPRGNSLKRLQEETLTKMSILGKGSMRDKAKEEELRKSGEAKYFHLHDDLHVLIEVFAPPAEAYARMGHALEEIKKFLIPDYNDEIRQAQLQELTYLNGGSENADVPVVRGKSTLRTRGVPTPAITRGRGGVTARPVGVGVPRGTPTSRGVLSTRGPVSRGRGLLTPRARGVPPTGYRPPPPPPTQETYGDYDYDDGYGTAYDEQSYDSYDNSYSTPVQSGADYYDYGHGLSEETYDSYGQEEWTNSRHKAPSARTAKGVYRDQPYGRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N1Y4</accession>
    <name>A0A8C4N1Y4_EQUAS</name>
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        <recommendedName>
            <fullName evidence="22">Interleukin-1 receptor type 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">CD121 antigen-like family member A</fullName>
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        <alternativeName>
            <fullName evidence="23">Interleukin-1 receptor alpha</fullName>
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        <alternativeName>
            <fullName evidence="26">Interleukin-1 receptor type I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">p80</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">IL1R1</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the coreceptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Involved in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">The interleukin-1 receptor complex is a heterodimer of IL1R1 and IL1RAP. Interacts with PIK3R1. Interacts with IL1A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the interleukin-1 receptor family.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSEASP00005032328.2"/>
        <property type="gene ID" value="ENSEASG00005022039.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01090000259985"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019966">
        <property type="term" value="F:interleukin-1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004909">
        <property type="term" value="F:interleukin-1, type I, activating receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050727">
        <property type="term" value="P:regulation of inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20991">
        <property type="entry name" value="Ig1_IL1R_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000002">
        <property type="entry name" value="Interleukin 1 receptor accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001064">
        <property type="entry name" value="Interleukin 1 receptor, type I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000188">
        <property type="entry name" value="Interleukin-1 receptor accessory protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000284">
        <property type="entry name" value="interleukin-1 receptor accessory protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015621">
        <property type="entry name" value="IL-1_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004076">
        <property type="entry name" value="IL-1_rcpt_I-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004074">
        <property type="entry name" value="IL-1_rcpt_I/II-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890:SF26">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01538">
        <property type="entry name" value="INTRLEUKN1R1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01536">
        <property type="entry name" value="INTRLKN1R12F"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="19" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="18" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="14 28" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 29" id="KW-0732">Signal</keyword>
    <keyword evidence="8 28" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 28" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="29">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040104879" description="Interleukin-1 receptor type 1" evidence="29">
        <location>
            <begin position="21"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="28">
        <location>
            <begin position="338"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="31">
        <location>
            <begin position="5"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="31">
        <location>
            <begin position="118"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="31">
        <location>
            <begin position="226"/>
            <end position="328"/>
        </location>
    </feature>
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        <location>
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            <end position="538"/>
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    </feature>
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            <dbReference type="PROSITE" id="PS50835"/>
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    <sequence length="573" mass="65951" checksum="05497D74A6D93A21" modified="2023-09-13" version="2">MKVLPRLVCFIALLISSLEADKCEEREEPIVLVSSAYEIDVRSCPLNPNESNGTIIWYKNDSETPVSMERDSRIHQYKDKLWFVPAKIEDSGHYYCAVRNSTYCLKVKITARFVQHEPDLCYNAQAIFTQKLPLAEDGLLVCPYLEVFRDENNELPEIQWYKDCQPLLLDNINFIGKTDKLIVANVTEAHKGHYTCHISYTHLGKQYPITRVIGLITLDEIRPTKPLIVSPVNETMEVDLGSQVQLICNVTGMFTDFVYWRWNGSLIDDSDPVLVEEYKPVENPSLKRRHTLITVLNISAVESRFYLYPFTCLAKNSYGRSAAYVQLRQPVPDFHKHVIGIFVLLTVAITCSVFIYKLFKVDLVLWYRDSCYDFRPPKASDGKTYDAYILYPKTLGEGSTSNSDIFVFKVLPEVLEKQCGYKLFIYGRDDYVGEDIVEVTNENIKKSRRLIIILVRETSGLSWLGSSSEEQIAMYNALVQDGIKIILLELEKIQDYEKMPESIKFIKRKHGALRWSGDFRKGPQSAKTRFWKNVRYRMPVQRQLPSSKCQLLSPATRPDSKEKLQGEVHVPLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PGA2</accession>
    <name>A0A8C4PGA2_EQUAS</name>
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        <recommendedName>
            <fullName evidence="5">Phosphatidylinositol-glycan biosynthesis class W protein</fullName>
            <ecNumber evidence="5">2.3.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PIGW</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="5">A acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Endoplasmic reticulum membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="gene ID" value="ENSEASG00005002139.2"/>
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        <property type="protein sequence ID" value="ENSEASP00005058217.1"/>
        <property type="gene ID" value="ENSEASG00005002139.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005061893.1">
        <property type="protein sequence ID" value="ENSEASP00005036626.1"/>
        <property type="gene ID" value="ENSEASG00005002139.2"/>
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    <dbReference type="Ensembl" id="ENSEAST00005076345.1">
        <property type="protein sequence ID" value="ENSEASP00005042917.1"/>
        <property type="gene ID" value="ENSEASG00005002139.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106844691"/>
    <dbReference type="KEGG" id="eai:106844691"/>
    <dbReference type="CTD" id="284098"/>
    <dbReference type="GeneTree" id="ENSGT00390000013520"/>
    <dbReference type="OMA" id="GLYVMQP"/>
    <dbReference type="UniPathway" id="UPA00196"/>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032216">
        <property type="term" value="F:glucosaminyl-phosphatidylinositol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009447">
        <property type="entry name" value="PIGW/GWT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF06423">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017321">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="74"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="5">
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            <begin position="80"/>
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    <feature type="transmembrane region" description="Helical" evidence="5">
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    <feature type="transmembrane region" description="Helical" evidence="5">
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            <begin position="448"/>
            <end position="465"/>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="471"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU280819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="503" mass="56554" checksum="6B96C4379DB6AF3B" modified="2022-01-19" version="1">MSQKQMKEAFVSNHNGTSVLEITQGLCLPALCVLCRGLLIILSQHLCSSSHTWGTRFFIDFVILIVPLVATLTILSSFVLLEHLAVIIIGAGMFYHIYCRRTCYARIPVQKILEKFLKISVESEYIPAISCFRVINSAFTAVAILAVDFPLFPRRFAKTELYGTGAMDFGVGGFVFGTAMVSPEVRRKYMKGSTFYHLTKSLYSVWPLVFLGIGRLVVIKSIDYQEHLTEYGVHWNFFFTLIVVKLITSLLLIIFPLNKSWIVAISITVLYQLALDFTPLRKLILYGTDGSGTRVGLLNANREGIISTLGYVAIHMAGVQTGSYVLKKRSHIKDWIKVAYCILLTAISLFISLYIVQVNVEVASRRMANLAFCIWIVASSLILLSSLLLVDIILSFAKFLIKGAQVPCSWKLIQSPATNKKHSESLVSEAERREPSLCLITAMNRNQLIFFLLSNVTTGLVNLLVDTLHSSTLWALFVLNLYMFTNCLVIYVLHLQDKTIKFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PJ06</accession>
    <name>A0A8C4PJ06_EQUAS</name>
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        <recommendedName>
            <fullName evidence="11">Dynein light intermediate chain</fullName>
        </recommendedName>
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        <name evidence="13" type="primary">DYNC1LI1</name>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the dynein light intermediate chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PJ06"/>
    <dbReference type="Ensembl" id="ENSEAST00005009378.2">
        <property type="protein sequence ID" value="ENSEASP00005008602.2"/>
        <property type="gene ID" value="ENSEASG00005006183.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008295"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030666">
        <property type="term" value="C:endocytic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045504">
        <property type="term" value="F:dynein heavy chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019003">
        <property type="term" value="F:GDP binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061502">
        <property type="term" value="P:early endosome to recycling endosome transport"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000226">
        <property type="term" value="P:microtubule cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090267">
        <property type="term" value="P:positive regulation of mitotic cell cycle spindle assembly checkpoint"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060627">
        <property type="term" value="P:regulation of vesicle-mediated transport"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008467">
        <property type="entry name" value="Dynein1_light_intermed_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022780">
        <property type="entry name" value="Dynein_light_int_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12688:SF2">
        <property type="entry name" value="CYTOPLASMIC DYNEIN 1 LIGHT INTERMEDIATE CHAIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12688">
        <property type="entry name" value="DYNEIN LIGHT INTERMEDIATE CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05783">
        <property type="entry name" value="DLIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 11" id="KW-0243">Dynein</keyword>
    <keyword evidence="5 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="469"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="540"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="494"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="12">
        <location>
            <begin position="540"/>
            <end position="560"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="Ensembl" id="ENSEASP00005008602.2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PJ15</accession>
    <name>A0A8C4PJ15_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">IF rod domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PJ15"/>
    <dbReference type="Ensembl" id="ENSEAST00005010072.2">
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        <property type="gene ID" value="ENSEASG00005006623.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153980"/>
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        <property type="term" value="C:intermediate filament"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0030855">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000002">
        <property type="entry name" value="Type I keratin 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000002">
        <property type="entry name" value="Type I keratin KA11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002957">
        <property type="entry name" value="Keratin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239">
        <property type="entry name" value="INTERMEDIATE FILAMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23239:SF97">
        <property type="entry name" value="KERATIN, TYPE I CUTICULAR HA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01248">
        <property type="entry name" value="TYPE1KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="5">
        <location>
            <begin position="45"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="49"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="155"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="248"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009260.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="391" mass="44727" checksum="5A158C314F4A2751" modified="2023-09-13" version="2">MSYSCFLPSLSCRSTCSSRPCVPPSCHGCTLPGACNIPGSFNGNEKETMQFLNDRLASYLEKVRQLERENAELESRIRERCQQQEPELCSNYQSYFRTIEELQQKILVSKSENARLVVQVDNAKLAADDFRTKYQTELGLRQLVESDINGLRRILDELTLCRSDLEAQVESLKEELISLKQNHEQEVNSLRSQLGDRLNVEVDAAPTVDLNRVLNETRRTWRNGSSSRWASQHTEELNRQVVSSSEQLLSYQAEIIELRRTVNALEIELQAQHNLRDSLENTLTETEARYSSQLSQVQGLITNVESQLAEIRSDLERQNQEYQVLLDVRARLESEINTYRGGLLESEDGVLKVVRTSQGTPGRGGSFVQKPCPRQYEGRVERGQQGWKRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PJW3</accession>
    <name>A0A8C4PJW3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Keratin-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106847358</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">In the hair cortex, hair keratin intermediate filaments are embedded in an interfilamentous matrix, consisting of hair keratin-associated proteins (KRTAP), which are essential for the formation of a rigid and resistant hair shaft through their extensive disulfide bond cross-linking with abundant cysteine residues of hair keratins. The matrix proteins include the high-sulfur and high-glycine-tyrosine keratins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with hair keratins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PMG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PJW3"/>
    <dbReference type="Ensembl" id="ENSEAST00005012064.2">
        <property type="protein sequence ID" value="ENSEASP00005011094.1"/>
        <property type="gene ID" value="ENSEASG00005007805.2"/>
    </dbReference>
    <dbReference type="KEGG" id="eai:106847358"/>
    <dbReference type="GeneTree" id="ENSGT00940000161951"/>
    <dbReference type="OMA" id="YRTCQSP"/>
    <dbReference type="OrthoDB" id="9835168at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007951">
        <property type="entry name" value="KRTAP_PMG"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05287">
        <property type="entry name" value="PMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0416">Keratin</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="169" mass="18147" checksum="3C6966C9A2289A67" modified="2022-01-19" version="1">MSYSCCSGNFSSCSLGSYLRYPGSSCGSSYPSNLVYSTDLCSPSTCQLGSSLYSGCGETCCEPTRSCVVSSPCQRSCYHPRISTVCSPCRSTYARSLGFGSSSCCSLGYGSRISYSRGCGSRGFRHLSCGVCGFPSLGYRSRFCHPTFLASRSCQTSCFRPTCRSGFYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PLV9</accession>
    <name>A0A8C4PLV9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">OR3A1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PLV9"/>
    <dbReference type="Ensembl" id="ENSEAST00005016739.2">
        <property type="protein sequence ID" value="ENSEASP00005015386.2"/>
        <property type="gene ID" value="ENSEASG00005030467.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162835"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000010">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48001">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="149"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="206"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="50"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005015386.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="296" mass="32528" checksum="85F98B55524E0353" modified="2023-09-13" version="2">VSLQERMQPESGINGTGITEFILLGLVETPGLWPVVFILFLFAYMVTVGSNLSILAAILVESKLHTPMYFFLGNLSVLDVGCITVTVPSMLGRLLSHKCTIPYEACLTQLFFFHLLVGVDCFLLTAMAYDRFLAICQPLTYNTRMSQTVQRMLVAVSWACAFTNALTHTVAVSTLNFCGPSVINHFYCDLPQLFQLSCSSTQLNELLLFAVGFIMAGTPLALVVTSYAHVAAAVLRIRSVEGRKKAFSTCGSHLTVVAIFYGSGIFNYMRLGSANLRNPDVQGALWRVLRGRWSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PMP8</accession>
    <name>A0A8C4PMP8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chromosome X open reading frame 38</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CXorf38</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="Ji"/>
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                <person name="Jin"/>
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                <person name="Yue"/>
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                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PMP8"/>
    <dbReference type="Ensembl" id="ENSEAST00005017938.2">
        <property type="protein sequence ID" value="ENSEASP00005016511.2"/>
        <property type="gene ID" value="ENSEASG00005011443.2"/>
    </dbReference>
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        <property type="component" value="Chromosome X"/>
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        <property type="entry name" value="DUF4559"/>
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        <property type="entry name" value="RGD1565685 PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RGD1565685 PROTEIN"/>
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        <location>
            <begin position="301"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005016511.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="320" mass="36574" checksum="DF558538C27CB6DA" modified="2023-09-13" version="2">MVPSELAARLNCAEYKNWVKAGHCLLLLRGCLQGFVGREVLAFHRTLLAAAPGLGPQAACRSGSRCSPRARQFQPQCQVCAAWKQEILKHHTNRNGDVYWGNCRPGRWPVDAWEVAKAFMPRGLADKTGPEECDAVALLSLINSCDHFMVDRKKVTEVIKCRNEIMHSSEMKVSSVWLRDFQMKIRNFLNEFTNIPEIVAVYSRIEQLLTSDWAVHIPEEDERDGCNYETGVYLSENQINEIEMELLKEKLQEMYLQAKEQEVLPEEISNRLEVLKEFLKDNEDLRSGLTEDMQNLDSFHLQHQKPDSEEPGAQTPEGKA</sequence>
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    <accession>A0A8C4PRQ1</accession>
    <name>A0A8C4PRQ1_EQUAS</name>
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        <recommendedName>
            <fullName evidence="1">Translationally-controlled tumor protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="6" type="primary">TPT1</name>
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        <name type="scientific">Equus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Zhao"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Zhuang"/>
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                <person name="Li"/>
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                <person name="Ju"/>
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                <person name="Li"/>
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                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Jordana"/>
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                <person name="Noce"/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in calcium binding and microtubule stabilization. Acts as a negative regulator of TSC22D1-mediated apoptosis, via interaction with and destabilization of TSC22D1 protein.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer. Interacts with STEAP3. Interacts with TSC22D1; interaction results in the destabilization of TSC22D1 protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TCTP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014694362.1">
        <property type="nucleotide sequence ID" value="XM_014838876.3"/>
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    <dbReference type="SMR" id="A0A8C4PRQ1"/>
    <dbReference type="Ensembl" id="ENSEAST00005027237.2">
        <property type="protein sequence ID" value="ENSEASP00005025087.1"/>
        <property type="gene ID" value="ENSEASG00005017078.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106829659"/>
    <dbReference type="KEGG" id="eai:106829659"/>
    <dbReference type="CTD" id="7178"/>
    <dbReference type="GeneTree" id="ENSGT00390000006051"/>
    <dbReference type="OMA" id="CAMITEG"/>
    <dbReference type="OrthoDB" id="5988874at2759"/>
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        <property type="component" value="Chromosome 11"/>
    </dbReference>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Tumor protein, translationally-controlled 1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.10">
        <property type="entry name" value="Metal Binding Protein, Guanine Nucleotide Exchange Factor, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011323">
        <property type="entry name" value="Mss4/transl-control_tumour"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034737">
        <property type="entry name" value="TCTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018103">
        <property type="entry name" value="Translation_control_tumour_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018105">
        <property type="entry name" value="Translational_control_tumour_p"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991">
        <property type="entry name" value="TRANSLATIONALLY CONTROLLED TUMOR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11991:SF0">
        <property type="entry name" value="TRANSLATIONALLY-CONTROLLED TUMOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00838">
        <property type="entry name" value="TCTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01653">
        <property type="entry name" value="TCTPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TCTP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01003">
        <property type="entry name" value="TCTP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51797">
        <property type="entry name" value="TCTP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TCTP" evidence="5">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00040832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00046053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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    <name>A0A8C4PSR6_EQUAS</name>
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            <fullName evidence="7">Alpha-tubulin N-acetyltransferase 1</fullName>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
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                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
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                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
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                <person name="J."/>
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                <person name="Yin"/>
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                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5 7">Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>L-lysyl-[alpha-tubulin] + acetyl-CoA = N(6)-acetyl-L-lysyl-[alpha-tubulin] + CoA + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:11278"/>
            <dbReference type="Rhea" id="RHEA-COMP:11279"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
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        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Component of the BBSome complex. Interacts with AP2 alpha-adaptins, including AP2A2, but not with AP1 gamma-adaptin (AP1G1/AP1G2); this interaction is required for efficient alpha-tubulin acetylation, hence clathrin-coated pits are sites of microtubule acetylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
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        <subcellularLocation>
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            <location evidence="7">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cell projection</location>
            <location evidence="7">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
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        </subcellularLocation>
        <subcellularLocation>
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            <location evidence="7">Cytoskeleton</location>
            <location evidence="7">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="7">Autoacetylation strongly increases tubulin acetylation.</text>
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    <comment type="similarity">
        <text evidence="7">Belongs to the acetyltransferase ATAT1 family.</text>
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        <property type="entry name" value="Acetyltransf_16"/>
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    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme with higher activity toward C18 acyl-CoAs, especially C18:0 acyl-CoAs. May participate to the production of saturated and monounsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 11">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Lipid metabolism; polyunsaturated fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The C-terminal di-lysine motif may confer endoplasmic reticulum localization.</text>
    </comment>
    <comment type="PTM">
        <text evidence="10">N-Glycosylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family. ELOVL3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.199" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0I7D0"/>
    <dbReference type="Ensembl" id="ENSEAST00005071643.1">
        <property type="protein sequence ID" value="ENSEASP00005036297.1"/>
        <property type="gene ID" value="ENSEASG00005027010.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244965"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035338">
        <property type="term" value="P:long-chain fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03203">
        <property type="entry name" value="VLCF_elongase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033679">
        <property type="entry name" value="ELOVL3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF68">
        <property type="entry name" value="ELONGATION OF VERY LONG CHAIN FATTY ACIDS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10 11" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10 11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 10 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="77"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="241"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Di-lysine motif" evidence="10">
        <location>
            <begin position="310"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005036297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="314" mass="36588" checksum="74E6930249668288" modified="2023-09-13" version="1">MQTEVNAIVGRARRRRACPGSLYPWSSNLFPSSRSPRPQRLQNEMVTAMNVSEETGQVLQPYSFELFRDMRPFLEEYWAASFLVVLIYLLLIFVGQNYMKARKGFSLRGPLILWSFCLAVFSILGAVRTWSHMGILLLRGNLGQTVCFSSHIQDPVVRCWSCLFLFSKIIELGDTAFIILRKRPLSFVHWYHHSTVLLYTSYGYKDKVSSGAWFMTMNYGVHAIMYTYYTLKAAKVKSPRWFPMLITSLQILQMFMGAFVSILNHIWRQDPGCHSTSEQLFWSCVLYITYFILFARFFHQTYLMPKVKAKTKSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I7Y1</accession>
    <name>A0A9L0I7Y1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AU RNA binding methylglutaconyl-CoA hydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">AUH</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014710156.2">
        <property type="nucleotide sequence ID" value="XM_014854670.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0I7Y1"/>
    <dbReference type="SMR" id="A0A9L0I7Y1"/>
    <dbReference type="Ensembl" id="ENSEAST00005071439.1">
        <property type="protein sequence ID" value="ENSEASP00005035976.1"/>
        <property type="gene ID" value="ENSEASG00005013993.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106839741"/>
    <dbReference type="KEGG" id="eai:106839741"/>
    <dbReference type="CTD" id="549"/>
    <dbReference type="GeneTree" id="ENSGT00940000157484"/>
    <dbReference type="OMA" id="YEQAHAW"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004490">
        <property type="term" value="F:methylglutaconyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000022">
        <property type="entry name" value="methylglutaconyl-CoA hydratase, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000001">
        <property type="entry name" value="Probable enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF12">
        <property type="entry name" value="METHYLGLUTACONYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005035976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="335" mass="35578" checksum="554A5B35EC0FA328" modified="2023-09-13" version="1">MAAAPGALGAWQAGRARLVAACCARLGPGSRLPDSLMGRQVGPAMWARGWAPAAGGAAPRRGYSSEVKTEDELRVRYLEEENRGIVVLGINRAYAKNSFSKNLIKMLSKAVDALKSDKKVRTIIVRSEVPGIFCAGADLKERVKMNSSEVGPFVSKIRAVINEIANLPVPTIAAIDGLALGGGLELALACDIRVAASSAKMGLVETKLAIIPGGGGTQRLPRAIGMSLAKELIFSARVLDGQEAKAVGLVSHVLEQNQEGDAAYRKALDLAREFLPQGPVAMRVAKLAINQGMEVDLVTGLAIEEACYAQTIPTKDRLEGLLAFKEKRPPRYKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I979</accession>
    <name>A0A9L0I979_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tribbles homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TRIB1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="3">Monomer. Interacts (via protein kinase domain) with CEBPA. Interacts with COP1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014709529.1">
        <property type="nucleotide sequence ID" value="XM_014854043.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0I979"/>
    <dbReference type="Ensembl" id="ENSEAST00005081613.1">
        <property type="protein sequence ID" value="ENSEASP00005036581.1"/>
        <property type="gene ID" value="ENSEASG00005035610.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106839369"/>
    <dbReference type="KEGG" id="eai:106839369"/>
    <dbReference type="CTD" id="10221"/>
    <dbReference type="GeneTree" id="ENSGT00950000182986"/>
    <dbReference type="OMA" id="GPPDCLS"/>
    <dbReference type="OrthoDB" id="410920at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031434">
        <property type="term" value="F:mitogen-activated protein kinase kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140416">
        <property type="term" value="F:transcription regulator inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055106">
        <property type="term" value="F:ubiquitin-protein transferase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046329">
        <property type="term" value="P:negative regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031665">
        <property type="term" value="P:negative regulation of lipopolysaccharide-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045659">
        <property type="term" value="P:negative regulation of neutrophil differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014912">
        <property type="term" value="P:negative regulation of smooth muscle cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048662">
        <property type="term" value="P:negative regulation of smooth muscle cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045645">
        <property type="term" value="P:positive regulation of eosinophil differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045651">
        <property type="term" value="P:positive regulation of macrophage differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032496">
        <property type="term" value="P:response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14023">
        <property type="entry name" value="PK_TRB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000381">
        <property type="entry name" value="tribbles homolog 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000153">
        <property type="entry name" value="Tribbles homolog 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024105">
        <property type="entry name" value="TRB1_pseudokinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024104">
        <property type="entry name" value="Tribbles/Ser_Thr_kinase_40"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22961">
        <property type="entry name" value="SER/THR PROTEIN KINASE-TRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22961:SF17">
        <property type="entry name" value="TRIBBLES HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="71"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="48"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="59"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00038180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00065559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00073884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005036581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="372" mass="40998" checksum="80974FDCD53BC64C" modified="2023-09-13" version="1">MRVGPVRSAMSGAAQPRGPALLLPAPRGAPTKRLLDADDAAAVAAKCPRLSECSSPPDYLSPPGSPCSPQPPPAAPVSGGGAGSAPGPSRIADYLLLPLAEREHVSRALCIHTGRELRCKVFPIKHYQDKIRPYIQLPSHRNITGIVEVILGETKAYVFFERDFGDMHSYVRSRKRLREEEAARLFKQIVSAVAHCHQSAIVLGDLKLRKFVFSTEERTQLRLESLEDTHIIKGEDDALSDKHGCPAYVSPEILNTTGTYSGKAADVWSLGVMLYTLLVGRYPFHDSDPSALFSKIRRGQFCIPDHISPKARCLIRSLLRREPSERLAAPDILLHPWFESVLEAGYVDSEMGTSDQIVPEYQEDSDISSFFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I9I3</accession>
    <name>A0A9L0I9I3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Xaa-Pro aminopeptidase 1</fullName>
            <ecNumber evidence="6">3.4.11.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Aminoacylproline aminopeptidase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Cytosolic aminopeptidase P</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Soluble aminopeptidase P</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">X-Pro aminopeptidase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">X-prolyl aminopeptidase 1, soluble</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">XPNPEP1</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Metalloaminopeptidase that catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro. Contributes to the degradation of bradykinin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.</text>
            <dbReference type="EC" id="3.4.11.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 22">Belongs to the peptidase M24B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.9" evidence="6"/>
    <dbReference type="RefSeq" id="XP_014685328.2">
        <property type="nucleotide sequence ID" value="XM_014829842.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0I9I3"/>
    <dbReference type="Ensembl" id="ENSEAST00005047441.1">
        <property type="protein sequence ID" value="ENSEASP00005036731.1"/>
        <property type="gene ID" value="ENSEASG00005014994.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106823890"/>
    <dbReference type="KEGG" id="eai:106823890"/>
    <dbReference type="CTD" id="7511"/>
    <dbReference type="GeneTree" id="ENSGT00940000157716"/>
    <dbReference type="OMA" id="EPGMILS"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010815">
        <property type="term" value="P:bradykinin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140633">
        <property type="term" value="P:CARD8 inflammasome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043069">
        <property type="term" value="P:negative regulation of programmed cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01085">
        <property type="entry name" value="APP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000001">
        <property type="entry name" value="Putative xaa-Pro aminopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.350.10:FF:000004">
        <property type="entry name" value="xaa-Pro aminopeptidase 1 isoform X1"/>
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        <property type="entry name" value="xaa-Pro aminopeptidase 1 isoform X1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
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    </dbReference>
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        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
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    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
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    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
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    <dbReference type="InterPro" id="IPR033740">
        <property type="entry name" value="Pept_M24B"/>
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    <dbReference type="InterPro" id="IPR032416">
        <property type="entry name" value="Peptidase_M24_C"/>
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    <dbReference type="InterPro" id="IPR001131">
        <property type="entry name" value="Peptidase_M24B_aminopep-P_CS"/>
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    <dbReference type="InterPro" id="IPR050422">
        <property type="entry name" value="X-Pro_aminopeptidase_P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43763:SF6">
        <property type="entry name" value="XAA-PRO AMINOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01321">
        <property type="entry name" value="Creatinase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16189">
        <property type="entry name" value="Creatinase_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16188">
        <property type="entry name" value="Peptidase_M24_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53092">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00491">
        <property type="entry name" value="PROLINE_PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="10 22" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Creatinase N-terminal" evidence="24">
        <location>
            <begin position="56"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24" evidence="23">
        <location>
            <begin position="371"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M24 C-terminal" evidence="25">
        <location>
            <begin position="600"/>
            <end position="662"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001424"/>
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    <evidence type="ECO:0000256" key="2">
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            <dbReference type="ARBA" id="ARBA00001936"/>
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    <evidence type="ECO:0000256" key="3">
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            <dbReference type="ARBA" id="ARBA00004496"/>
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    <sequence length="666" mass="74769" checksum="F9E75D6F9B275FE3" modified="2023-09-13" version="1">MAASRKAPRVRANHQDFLLKNLRIIEPNEVTHTGDPGVETDSRMAPKVTSELLRQLRQAMRNLEYVTEPIQAYIIPSGDAHQSEYIAPCDCRRAFVSGFDGSAGTAIITEEHAAMWTDGRYFLQAAKQMDSNWTLMKMGLKDTPTQEDWLVSVLPEGSRVGVDPLIIPTDYWKKMAKVLRSAGHHLIPVKENLVDKIWTDRPERPCKPLLTLGLDYTGISWKDKVADLRLKMAERNVVWFVVTALDEIAWLFNLRGSDVEHNPVFFSYAVIGLETIMLFIDGDRIDTPSVKEHLLLDLDLEAEYRIQVLPYKSILSKLKALCADLSPREKVWVSDKASYAVSEAIPKDHRCCMPYTPICIAKAVKNSAESEGMRRAHIKDAVALCELFNWLEKEVPKGGVTEISAADKAEEFRRQQADFVDLSFPTISSTGPNGAIIHYAPVPETNRTLSLDEVYLIDSGAQYKDGTTDVTRTMHFGTPKAYEKECFTYVLKGHIAVSAAVFPTGTKGHLLDSFARSALWDSGLDYLHGTGHGVGSFLNVHEGPCGISYKTFSDEPLEAGMIVTDEPGYYEDGAFGIRIENVVLVVPVKTKYNFNNRGSLTFEPLTLVPIQTKMIDVDSLTDKECDWLNNYHLTCRDVVGKELQKQGRQEALEWLIRETQPISRQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IAW3</accession>
    <name>A0A9L0IAW3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Tenascin XB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TNXB</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tenascin family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005072182.1">
        <property type="protein sequence ID" value="ENSEASP00005037957.1"/>
        <property type="gene ID" value="ENSEASG00005014360.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155565"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098633">
        <property type="term" value="F:collagen fibril binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030199">
        <property type="term" value="P:collagen fibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032963">
        <property type="term" value="P:collagen metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048251">
        <property type="term" value="P:elastic fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031175">
        <property type="term" value="P:neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904028">
        <property type="term" value="P:positive regulation of collagen fibril organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030155">
        <property type="term" value="P:regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046328">
        <property type="term" value="P:regulation of JNK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="29"/>
    </dbReference>
    <dbReference type="CDD" id="cd00087">
        <property type="entry name" value="FReD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000001">
        <property type="entry name" value="Tenascin C"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.215.10:FF:000001">
        <property type="entry name" value="Tenascin isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000024">
        <property type="entry name" value="Tenascin-X"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000701">
        <property type="entry name" value="Tenascin-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000760">
        <property type="entry name" value="tenascin-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000794">
        <property type="entry name" value="Tenascin-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000883">
        <property type="entry name" value="tenascin-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.215.10">
        <property type="entry name" value="Gamma Fibrinogen, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="31"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050991">
        <property type="entry name" value="ECM_Regulatory_Proteins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Liu"/>
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                <person name="X. and Zhong"/>
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    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IBI0"/>
    <dbReference type="Ensembl" id="ENSEAST00005083713.1">
        <property type="protein sequence ID" value="ENSEASP00005035120.1"/>
        <property type="gene ID" value="ENSEASG00005006091.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106825988"/>
    <dbReference type="GeneTree" id="ENSGT00940000161865"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000681">
        <property type="entry name" value="zinc finger protein 205 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000725">
        <property type="entry name" value="zinc finger protein 205 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000781">
        <property type="entry name" value="zinc finger protein 205 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000853">
        <property type="entry name" value="zinc finger protein 205 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000984">
        <property type="entry name" value="zinc finger protein 205 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001010">
        <property type="entry name" value="zinc finger protein 64 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000070">
        <property type="entry name" value="zinc finger protein 689 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000953">
        <property type="entry name" value="Zinc finger protein 691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF416">
        <property type="entry name" value="FI01424P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="15">
        <location>
            <begin position="124"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="304"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="332"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="360"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="388"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="416"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="444"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="472"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="500"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="174"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="224"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="174"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="186"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="273"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005035120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="548" mass="60380" checksum="1DA18F66EBBD3638" modified="2023-09-13" version="1">MSADGRGIRATQDKKKAQELPGPRHLCQEMLSEAEEVVPLGATRESPHIKMEPEEQHPEVVLQEDGTQGARGSVPLSLGSKEKALFLPGGALPSPQIPVLSREGRTRDRQMAAALLTAWSQMPVTFEDVALYLSREEWGRLDHTQQSFYRDVLQKRNGLSLGFPFGRPVWASQVQGKGEASSSCRQTDEEEKRGAIEVGKEEPAPSLAALRDVKALRTRAGRAQGDILQCRRQAANSQSPGPAKNDGQPGPPEERQPKPAPPDTSLLKAQKGHLPEKPKEGETGAPENSEESLPADGDTSKKTYKCEQCGKGFSWHSHLVTHRRTHTGEKPYACTDCGKRFGRSSHLIQHQIIHTGEKPYTCPSCWKSFSHHSTLIQHQRIHTGEKPYVCDRCAKRFTRRSDLVTHQGTHTGAKPHKCPICSKCFTQSSALVTHQRTHTGVKPYPCPECGKCFSQRSNLIAHNRTHTGEKPYHCLDCGKSFSHSSHLTAHQRTHRGVRPYSCPLCGKSFSRRSNLHRHEKIHTTGPKALAMLMLGAAGALAAPSPAPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IFC0</accession>
    <name>A0A9L0IFC0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="5">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">OTUD7A</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peptidase C64 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0IFC0"/>
    <dbReference type="Ensembl" id="ENSEAST00005083800.1">
        <property type="protein sequence ID" value="ENSEASP00005036465.1"/>
        <property type="gene ID" value="ENSEASG00005017841.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158999"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070530">
        <property type="term" value="F:K63-linked polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071947">
        <property type="term" value="P:protein deubiquitination involved in ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035871">
        <property type="term" value="P:protein K11-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070536">
        <property type="term" value="P:protein K63-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22773">
        <property type="entry name" value="OTU_OTUD7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14347">
        <property type="entry name" value="UBA_Cezanne_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000017">
        <property type="entry name" value="OTU domain-containing protein 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051346">
        <property type="entry name" value="OTU_Deubiquitinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13367:SF9">
        <property type="entry name" value="OTU DOMAIN-CONTAINING PROTEIN 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <source>
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                <person name="M."/>
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                <person name="D.D."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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        <source>
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                <person name="W."/>
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                <person name="R."/>
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                <person name="J."/>
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                <person name="X."/>
                <person name="Yin"/>
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                <person name="Xu"/>
                <person name="J."/>
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                <person name="Jordana"/>
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                <person name="A."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Participates in a complex which severs microtubules in an ATP-dependent manner. May act to target the enzymatic subunit of this complex to sites of action such as the centrosome. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with KATNA1. This interaction enhances the microtubule binding and severing activity of KATNA1 and also targets this activity to the centrosome. This interaction is weakly competed by KATNBL1 which has a lower affinity for it. Interacts with ASPM; the katanin complex formation KATNA1:KATNB1 is required for the association of ASPM. Interacts with dynein, microtubules, NDEL1 and PAFAH1B1. Interacts with KATNAL1; this interaction is weakly competed by KATNBL1 which has a lower affinity for it. Interacts with CAMSAP2 and CAMSAP3; leading to regulate the length of CAMSAP-decorated microtubule stretches.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Cytoskeleton</location>
            <location evidence="1 11">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Cytoskeleton</location>
            <location evidence="11">Microtubule organizing center</location>
            <location evidence="11">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Cytoskeleton</location>
            <location evidence="11">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
            <location evidence="11">Cytoskeleton</location>
        </subcellularLocation>
        <text evidence="11">Predominantly cytoplasmic. Localized to the interphase centrosome and mitotic spindle poles. Localizes within the cytoplasm, partially overlapping with microtubules, in interphase and to the mitotic spindle and spindle poles during mitosis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the WD repeat KATNB1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014682853.1">
        <property type="nucleotide sequence ID" value="XM_014827367.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014682854.1">
        <property type="nucleotide sequence ID" value="XM_014827368.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IGQ3"/>
    <dbReference type="Ensembl" id="ENSEAST00005053573.1">
        <property type="protein sequence ID" value="ENSEASP00005039372.1"/>
        <property type="gene ID" value="ENSEASG00005005438.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822252"/>
    <dbReference type="KEGG" id="eai:106822252"/>
    <dbReference type="CTD" id="10300"/>
    <dbReference type="GeneTree" id="ENSGT00940000157918"/>
    <dbReference type="OMA" id="AMDVQCP"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008352">
        <property type="term" value="C:katanin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007019">
        <property type="term" value="P:microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051013">
        <property type="term" value="P:microtubule severing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007079">
        <property type="term" value="P:mitotic chromosome movement towards spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031117">
        <property type="term" value="P:positive regulation of microtubule depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000348">
        <property type="entry name" value="Katanin p80 WD40 repeat-containing subunit B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000583">
        <property type="entry name" value="Katanin p80 WD40 repeat-containing subunit B1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03022">
        <property type="entry name" value="Katanin_p80_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028021">
        <property type="entry name" value="Katanin_C-terminal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026962">
        <property type="entry name" value="KTNB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19845">
        <property type="entry name" value="KATANIN P80 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19845:SF0">
        <property type="entry name" value="KATANIN P80 WD40 REPEAT-CONTAINING SUBUNIT B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13925">
        <property type="entry name" value="Katanin_con80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5 11" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 11" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 11" id="KW-0498">Mitosis</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="16"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="59"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="101"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="143"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="185"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Katanin p80 subunit C-terminal" evidence="14">
        <location>
            <begin position="493"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="312"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="342"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="655" mass="72226" checksum="751420CE55C9D3A6" modified="2023-09-13" version="1">MATPVVTKTAWKLQEIVAHASNVSSLVLGKASGRLLATGGDDCRVNLWSINKPNCIMSLTGHTSPVESVRLNTPEELIVAGSQSGSIRVWDLEAAKILRTLMGHKANICSLDFHPYGEFVASGSQDTNIKLWDIRRKGCVFRYRGHSQAVRCLRFSPDGKWLASAADDHTVKLWDLTAGKMMSEFPGHTGPVNVVEFHPNEYLLASGSSDRTIRFWDLEKFQVVSCIEGEPGPVRSVLFNPDGCCLYSGCQDSLRVYGWEPERCFDVVLVNWGKVADLAICNDQLIGVAFSQSNVSSYVVDLTRVTRTGMVAQDPVQDSRPLAQQPPHPSAPLRRIYERPSTACSKPQRVKQNSESERRSPSSEDDRDERESQAEIQNAEDYNEIFQPKNSISRTPPRRSEPFPAPPEDDVATAKEAAEPSPAMDAQFPVPNLEVLARPPVVTSTPAPKAEPTIIPAARNEPIGLKASDFLPAVKIPQQAELVDEDAMSQIRKGHDTMCVVLTSRHKNLDTVRAVWTTGDIKTSVDSAVAINDLSVVVDLLNIVNQKASLWKLDLCTTVLPQIEKLLQSKYESYVQTGCTSLKLILQRFLPLITDILAAPPSVGVDISREERLHKCRLCYKQLKSISGFVKSKSGLSGRHGSAFRELHLLMASLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IHH0</accession>
    <name>A0A9L0IHH0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">leucine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Leucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LARS2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>tRNA(Leu) + L-leucine + ATP = L-leucyl-tRNA(Leu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11688"/>
            <dbReference type="Rhea" id="RHEA-COMP:9613"/>
            <dbReference type="Rhea" id="RHEA-COMP:9622"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78494"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.4" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0IHH0"/>
    <dbReference type="Ensembl" id="ENSEAST00005063185.1">
        <property type="protein sequence ID" value="ENSEASP00005040390.1"/>
        <property type="gene ID" value="ENSEASG00005008052.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015114"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004823">
        <property type="term" value="F:leucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006429">
        <property type="term" value="P:leucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07958">
        <property type="entry name" value="Anticodon_Ia_Leu_BEm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00812">
        <property type="entry name" value="LeuRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000011">
        <property type="entry name" value="Leucine--tRNA ligase chloroplastic/mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000612">
        <property type="entry name" value="Leucyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000023">
        <property type="entry name" value="probable leucine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000100">
        <property type="entry name" value="probable leucine--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002302">
        <property type="entry name" value="Leu-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025709">
        <property type="entry name" value="Leu_tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00396">
        <property type="entry name" value="leuS_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740:SF2">
        <property type="entry name" value="LEUCINE--TRNA LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43740">
        <property type="entry name" value="LEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13603">
        <property type="entry name" value="tRNA-synt_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00985">
        <property type="entry name" value="TRNASYNTHLEU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12 15" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8 15" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="16">
        <location>
            <begin position="61"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Leucyl-tRNA synthetase editing" evidence="18">
        <location>
            <begin position="271"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="16">
        <location>
            <begin position="444"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="16">
        <location>
            <begin position="631"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="17">
        <location>
            <begin position="720"/>
            <end position="842"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF13603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005040390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="896" mass="101299" checksum="EAF5DF386F5E1BEF" modified="2023-09-13" version="1">MASPWQRLGFYASLLKRQLNGGPDVINWGKRVIPRHTRGIYSATGKWTKEYTLQTRKDVEKWWHQRIKEQASKISETDKSKPKFYVLSMFPYPSGKLHMGHVRVYTISDTIARFQKMRGMQVINPMGWDAFGLPAENAAIERNLHPESWTQSNIKHMRKQLDRLGLCFSWDREITTCLPDYYKWTQYLFIKLYEAGLAYQKEALVNWDPVDQTVLANEQVDEHGCSWRSGAKVEQKYLRQWFIKTTAYAKAMQDALADLPEWYGIKGMQAHWIGDCVGCHLDFTLKVDGQVTGEKLTAYTATPEAIYGTSHVAISPSHRLLHGHSSLKDAFRKALVPGRDCLTPVTAVNMLTQQELPVVILARADFQGSLDSKIGIPSTSSEDTILAQTLGLSYSEVIETLPDGTERLSNSAEFTGMTRQDAFLALTQKARRKRVGGDVTSDKLKDWLISRQRYWGTPIPVVHCPACGPVPVPLEDLPVTLPSVTSFTGKGGSPLATASEWVNCSCPRCKGAAKRETDTMDTFVDSAWYYFRYTDPQNAHSPFSAALADYWMPVDLYIGGKEHAVMHLFYARFFSHFCHDQKMVKHREPFHKLLAQGLIKGQTFRLPSGQYVQREEVDLTETKEKLEVTWEKMSKSKHNGVDPEEVVEQYGLDTIRLYLLFAAPPEKDILWDVKTDALPGVLRWQQRLWSLATRFIEARASGKVPRPQLLNDEEKAEARKLWEYKNSVISQVTAHFTDDFSLNSAISHLMGLSSALLQASQKVVLHSPEFEDALCALMVMAAPMAPHITSELWAGLALVPRKLCAHYAWDAGVLLQAWPAVDPHFLQPPDVVQMAVLINNKACGKIPVPQQVAQDQDKVHELVLQSELGVRLLQGRSIKKAFLSPRTALINFLVQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IJK4</accession>
    <name>A0A9L0IJK4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PWWP domain containing 3A, DNA repair factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106846542</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PWWP3A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IJK4"/>
    <dbReference type="Ensembl" id="ENSEAST00005068629.1">
        <property type="protein sequence ID" value="ENSEASP00005037238.1"/>
        <property type="gene ID" value="ENSEASG00005028277.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001700"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="CDD" id="cd06080">
        <property type="entry name" value="PWWP_MUM1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000063">
        <property type="entry name" value="PWWP domain-containing DNA repair factor 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035504">
        <property type="entry name" value="MUM1-like_PWWP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040263">
        <property type="entry name" value="PWP3A_3B_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048795">
        <property type="entry name" value="PWP3A_3B_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048765">
        <property type="entry name" value="PWP3A_3B_4_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31333">
        <property type="entry name" value="PWWP DOMAIN-CONTAINING DNA REPAIR FACTOR 3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31333:SF2">
        <property type="entry name" value="PWWP DOMAIN-CONTAINING DNA REPAIR FACTOR 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20884">
        <property type="entry name" value="MUM1-like_PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20886">
        <property type="entry name" value="PWP3A-B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20887">
        <property type="entry name" value="PWP3A-B_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PWWP" evidence="5">
        <location>
            <begin position="25"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="MUM1-like PWWP" evidence="3">
        <location>
            <begin position="379"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="PWWP" evidence="4">
        <location>
            <begin position="530"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="127"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="285"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="326"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="141"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="213"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF20887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005037238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="685" mass="77232" checksum="00D0D339075EAA14" modified="2023-09-13" version="1">MTARPLHLYELPLDRKGKIIMDATYVLCNWKGLFWPAKVLSRSGIPPTNKRKKVLSLEVQILSVDEKIRVKSTDIKILNESQIESMASSLVAQSEASVPPGEEAAYRSALTVAWEILSERANLGRARALDDPETTTLSQKRLQKPSRRKYWKPRGNLPRSLGKSENPKSPWVRSEREGSKSQVHTAVARIPREMQTTSSQSSSVCPNFPSLSEDDREREGKEKRDSSRVMSLHCTAKEEGAGAQDGGVLPSPPPGFILTVPKALQEGTHNSGPKTLAVSSECSTFSEGVEDPGEGAWKPGLEGAAAASSAPNLRLRCSLRLANRKRKLQGPESERRLQELRPLVGSKAVNPTTTPKKDVGKEAGQPTSMALPREPGPIERGMMVWFKFQNHPFWPAVVKSVSQTEQTARVLLIEANMHLEKSGIQVPLRRLKHLDCKEKEKLIKRARKAYEQSVNWCFSLISHYREGLGRGCFAGSFLDYYAADVSYPIRKAIQEGTLEIDFPRVNYDDLDDSEEETSLGGKRPCKKILPDRMKAARDRANQKLVDFIVKRKGADHHLLDIVKGRKQSRWLASFLNSNRYVICVETYLEDEDQLDVVVRHLQEIYKQMDKRVLNRVRDDRVSFVVEVLLPEAIICSIAALDGLDYKEAEEKYRKGPPVHSREKELFDKNLLKEMRKRSARRSKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IM63</accession>
    <name>A0A9L0IM63_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription factor HES-7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Hairy and enhancer of split 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">bHLH factor Hes7</fullName>
        </alternativeName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Transcriptional repressor. Represses transcription from both N box- and E box-containing promoters. May with HES1, cooperatively regulate somite formation in the presomitic mesoderm (PSM). May function as a segmentation clock, which is essential for coordinated somite segmentation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Transcription repression requires formation of a complex with a corepressor protein of the Groucho/TLE family.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IM63"/>
    <dbReference type="Ensembl" id="ENSEAST00005041839.1">
        <property type="protein sequence ID" value="ENSEASP00005042036.1"/>
        <property type="gene ID" value="ENSEASG00005024043.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00730000111282"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000063">
        <property type="entry name" value="transcription factor HES-7 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050370">
        <property type="entry name" value="HES_HEY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10985">
        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="14">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="167"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="193"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="218"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005042036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="268" mass="28778" checksum="EC6C4B0F69495FE4" modified="2023-09-13" version="1">MQKTREREEMLKPLVEKRRRDRINRSLEELRLLLLERTRDQNLRNPKLEKAEILEFAVGYLRERSRVEPPGTARGWGSGGREGGFTEVRAGADGVGPAVDGGLGGWQALLRGEVNNTRACLSLSPSFSPSVGPSGFLSLSVRLSGFGISAPRPFPHPCWAPALPARPPAGCTRRRASRGSPVPSPGRRGARQLLLVRLPRVPASPGGLRARRQPGRPRPALLRAAWLPAPQAAPAGTGRSEAPSAAPTAGPRRPGAWPRAAPAPPRPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IMG2</accession>
    <name>A0A9L0IMG2_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Zinc finger protein 112</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ZNF112</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IMG2"/>
    <dbReference type="Ensembl" id="ENSEAST00005039892.1">
        <property type="protein sequence ID" value="ENSEASP00005038263.1"/>
        <property type="gene ID" value="ENSEASG00005013640.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162215"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000274">
        <property type="entry name" value="zinc finger protein 16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000006">
        <property type="entry name" value="Zinc finger protein 184 (Kruppel-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000671">
        <property type="entry name" value="Zinc finger protein 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000663">
        <property type="entry name" value="Zinc finger protein 45"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002357">
        <property type="entry name" value="Zinc finger protein 782"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF88">
        <property type="entry name" value="VOMERONASAL 1 RECEPTOR, F4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="16"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
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            <begin position="23"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50157"/>
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            <dbReference type="PROSITE" id="PS50805"/>
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    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038263.1"/>
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    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="926" mass="106483" checksum="43ED23475F66D0EB" modified="2023-09-13" version="1">MVNKVSAFPQEKEEKMIKVQEPVTFEDVAVAFTEEELGLLDATQRQLYRDVMLENFRNLLSVGNQPFKPELIFQLEREEKLSMMETETWRDGCSGTKNEHHMESIREVGLSCPSPKELSSWQNWQQGAGGLMRCQDSMNNFQGNISQVPKQGDSACQVWAEIPIQIPEDENYVLTHAEDGSTYITNQDFPSWRAQHSWRKTYLTESCNYQCRCQQISRKNNFCKCDSVSWMSHHNDNLRLHRTEKNYSCHDCGEDIMKVSLRNQDFLQTGQKPYPPNEYRESFSDDASSEIQQQFPSEGKPHTYSPRGKGCSYSSVLRIHQSVPRGDGCVSESSHLQSHRRVHTEEKPCKCEYGEDFSQCSPLDTYELHTGETSYRRTIYEKAFSHSLDLNSIFRVRTGGEPHEYEENGTVFNESSCLQVHQRMHAEEKLYTGVECEKGFICASNLNLQHRVHMEEIPYNSEECGNGFSLASRLQDLQIVHTREEPYKYYVCGNKFSQNAYLQGHQKIHLAEKPYKEFRNGFNWSSKLKDHQRVHGGEKPYKCNACGKGFSHRSVLTVHQRVHTGEKPYKCEECDKGFSRSSYLQAHQRVHTGEKPYKCEECGKGFSRNSYLQGHQRVHTGEKPYKCEECGKGFSRSSHLQGHQRVHTGEKPFKCEECGKGFSWSFNLQIHQRVHTGEKPYKCGECGKGFSKASTLLAHQRVHTGEKPYQCDECGKSFSQRSYLQSHQSVHTGERPYICEVCGKGFSQRAYLQGHQRVHTRVKPYKCEMCGKGFSQSSRLEAHRRVHTGGKPYKCEVCTKGFSESSRLQAHQRVHAEGRPYKCEQCGKGFSGFSSLQAHHRVHTGEKPYKCEVCGKGFSQRSNLQAHQRVHTGEKPYKCDACGKGFRWSSGLLIHQRVHSDAKFYKSEEDGKGYPSSENPYRHEVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IPU0</accession>
    <name>A0A9L0IPU0_EQUAS</name>
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        <recommendedName>
            <fullName evidence="9">Cationic amino acid transporter C-terminal domain-containing protein</fullName>
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        <name type="scientific">Equus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Zhao"/>
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                <person name="Wang"/>
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                <person name="Liu"/>
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                <person name="Q."/>
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                <person name="Yang"/>
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                <person name="Tian"/>
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                <person name="Yu"/>
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                <person name="A."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <property type="entry name" value="AA_transporter"/>
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            <end position="85"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="436"/>
            <end position="453"/>
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    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="9">
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            <begin position="434"/>
            <end position="471"/>
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    </feature>
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            <begin position="311"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
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            <begin position="328"/>
            <end position="340"/>
        </location>
    </feature>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
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    <sequence length="520" mass="56216" checksum="731E013E04BF9458" modified="2023-09-13" version="1">MCSWGNCGPSSLAGYCSSSVSLVGLPWPAPGAAHLDAILSHGIRSFTMAHVGSWQVPFLAQYPDFLAAGVILLASAFVYCGVHICSWLNRTFSAISLVVILFIVTLGFVLARPENWSTEEGGFAPFGFSGIMAGAATCFFAFLGFGAIAASSEEGQNPKRAVPMAIAISVGLMAGTNILASTVLTLMVPRHGLDPDWTLADAFYQRGYSWAGFIVAAGAVCCMTTVLFNILFAVPRIVHAMSTDGLFFQIFTRVHPRTQVPIVGILVFGFLMPLLAVLLDLEALIRFLSIGTLVTRPVVNTSIIVLRFQKSSPSSPLGSVSPGPVAEGYEHSSGHRRLEDTEQPSAAEPGQLRPALRPFLGFISGCRPGAAVAWALRVLVVSAIILDCVLAFGKSALHLPPWGHTLLLLLSSVVFLLSLLVLGAHQQQRRQDTFQVPMVPLIPAASILLNVFLMLHLSSLTWLRFSIWLLIAPCRAQRHKDNRILNEERLDTATSPLVLIGRLRERSQKVKRASLEIGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IWE8</accession>
    <name>A0A9L0IWE8_EQUAS</name>
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        <recommendedName>
            <fullName evidence="14">Protein arginine N-methyltransferase 6</fullName>
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        <alternativeName>
            <fullName evidence="15">Histone-arginine N-methyltransferase PRMT6</fullName>
        </alternativeName>
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        <name evidence="20" type="primary">PRMT6</name>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
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                <person name="Xu"/>
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                <person name="Liu"/>
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        <scope>IDENTIFICATION</scope>
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            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
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                <person name="Ju"/>
                <person name="Z."/>
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                <person name="J."/>
                <person name="Li"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <property type="entry name" value="FATTY ACID DESATURASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19353:SF22">
        <property type="entry name" value="FATTY ACID DESATURASE 2-LIKE PROTEIN FADS2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
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    <keyword evidence="4" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="167"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="298"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="338"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="8">
        <location>
            <begin position="57"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005042600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="453" mass="53263" checksum="93982F2DB09F3005" modified="2023-09-13" version="1">MKFEEKYEGNGRLVGKSQPYLYEKPQGNWKPVANEKAEASVKQEANGKCCDTPREILNTYSWQEIQRHNQESDQWLVINHKVYDVTGWAKRHPGGRWVLNHYAGDDAMDVFRATHPKLDVVQLYLKPLLIGELAPGEPSQERNKNSQLVEDFQELQRKLEAMNMFNANLGFFFLHLAQILILEALAWLMLWHFGNSWTITIFISFLLTVSQVQSAFLQHDAEHLSIFKKSKYNRLMHKFDGVSRNLWISAHPQHHGKLNIYSKDPDISFQPLFLFGDLLPVEYGKRKIKYINYEKQHLYFLMIVLPFFMPGYISMQTVRHIYNRKRWEELPWVSSFYIHYFIIFGPSYGIFGSLLLIYFVNHITTREENQDWLSTQVLATCNVEQSFFNDWFTGHLNFQIEHHLFPTMPRHNYHKVAPLVKSLCAKHGLQYVKKPLLKAVGDIFSKSDETWGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J0C2</accession>
    <name>A0A9L0J0C2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Equilibrative nucleoside transporter 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Plasma membrane monoamine transporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Solute carrier family 29 member 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">SLC29A4</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(R)-adrenaline(out) = (R)-adrenaline(in)</text>
            <dbReference type="Rhea" id="RHEA:73875"/>
            <dbReference type="ChEBI" id="CHEBI:71406"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(R)-noradrenaline(out) = (R)-noradrenaline(in)</text>
            <dbReference type="Rhea" id="RHEA:73871"/>
            <dbReference type="ChEBI" id="CHEBI:72587"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>adenosine(in) = adenosine(out)</text>
            <dbReference type="Rhea" id="RHEA:75343"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:75344"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="14">
            <dbReference type="Rhea" id="RHEA:75345"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>dopamine(out) = dopamine(in)</text>
            <dbReference type="Rhea" id="RHEA:73863"/>
            <dbReference type="ChEBI" id="CHEBI:59905"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>guanidine(out) = guanidine(in)</text>
            <dbReference type="Rhea" id="RHEA:73883"/>
            <dbReference type="ChEBI" id="CHEBI:30087"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>histamine(out) = histamine(in)</text>
            <dbReference type="Rhea" id="RHEA:73879"/>
            <dbReference type="ChEBI" id="CHEBI:58432"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>serotonin(out) = serotonin(in)</text>
            <dbReference type="Rhea" id="RHEA:73867"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tyramine(in) = tyramine(out)</text>
            <dbReference type="Rhea" id="RHEA:74783"/>
            <dbReference type="ChEBI" id="CHEBI:327995"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC29A/ENT transporter (TC 2.A.57) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J0C2"/>
    <dbReference type="Ensembl" id="ENSEAST00005050679.1">
        <property type="protein sequence ID" value="ENSEASP00005042765.1"/>
        <property type="gene ID" value="ENSEASG00005034570.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182898"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015562">
        <property type="term" value="F:efflux transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008504">
        <property type="term" value="F:monoamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005326">
        <property type="term" value="F:neurotransmitter transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005337">
        <property type="term" value="F:nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005342">
        <property type="term" value="F:organic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015101">
        <property type="term" value="F:organic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019534">
        <property type="term" value="F:toxin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032238">
        <property type="term" value="P:adenosine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990748">
        <property type="term" value="P:cellular detoxification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090494">
        <property type="term" value="P:dopamine uptake"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048241">
        <property type="term" value="P:epinephrine transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051625">
        <property type="term" value="P:epinephrine uptake"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140115">
        <property type="term" value="P:export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051615">
        <property type="term" value="P:histamine uptake"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051620">
        <property type="term" value="P:norepinephrine uptake"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051610">
        <property type="term" value="P:serotonin uptake"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000255">
        <property type="entry name" value="Solute carrier family 29 member 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002259">
        <property type="entry name" value="Eqnu_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10332:SF10">
        <property type="entry name" value="EQUILIBRATIVE NUCLEOSIDE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01733">
        <property type="entry name" value="Nucleoside_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016379">
        <property type="entry name" value="ENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01130">
        <property type="entry name" value="DERENTRNSPRT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="140"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="344"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="413"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="447"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00036483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00036754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00036845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00036998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00051479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005042765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="525" mass="57098" checksum="53DFD53FD8F66028" modified="2023-09-13" version="1">MGSVGSQRLREAGAASPWDRSMVMSFSFDGGQLEEVAVGTARGQADGPGGTPVSPDSAVEEPVPDDRYHAIYFAMLLAGVGFLLPYNSFITDVDFLHHKYPGTSIVFDMSLTYILVALVAVLLNNALVERLSLHTRITAGYLLALGPLLFVSICDVWLQLFSRDQAYAINLAAVGTVAFGCTVQQSSFYGYTGMLPKRYTQGVMTGESTAGVMVSLSRILTKLLLPDERASTLIFFLLSAGLELLCCLLHLLVRRSRFVRRHTARPRDCRPAGPRYRVHHDVAAGDVRFEHQGPAPANCGSPKDSPAHEVTSGCGGAYTRFDVPQRRVKRSWPSFRALLLHRYVVARVIWADMLSIAVTYFITLCLFPGLESEIRHCILGEWLPILIMAVFNLSDFVGKILAALPVDWRGPHLLACACLRLVFIPLFILCVYPSGAPALRHPAWPCVFSLLMGISNGYFGSVPMILAAGKVSPKQRELAGNTMTVSYMTGLTLGSAVAYGTYSLTRGARRSCFPATANASLPAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J0L2</accession>
    <name>A0A9L0J0L2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Uteroglobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106847595</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the secretoglobin family. Lipophilin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014722429.1">
        <property type="nucleotide sequence ID" value="XM_014866943.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J0L2"/>
    <dbReference type="Ensembl" id="ENSEAST00005079347.1">
        <property type="protein sequence ID" value="ENSEASP00005042850.1"/>
        <property type="gene ID" value="ENSEASG00005036713.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106847595"/>
    <dbReference type="KEGG" id="eai:106847595"/>
    <dbReference type="GeneTree" id="ENSGT00530000063866"/>
    <dbReference type="OrthoDB" id="9535440at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00633">
        <property type="entry name" value="Secretoglobin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016126">
        <property type="entry name" value="Secretoglobin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035960">
        <property type="entry name" value="Secretoglobin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11332">
        <property type="entry name" value="SECRETOGLOBIN FAMILY 1D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11332:SF6">
        <property type="entry name" value="SECRETOGLOBIN FAMILY 1D MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01099">
        <property type="entry name" value="Uteroglobin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48201">
        <property type="entry name" value="Uteroglobin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51311">
        <property type="entry name" value="SCGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040113131" description="Uteroglobin" evidence="5">
        <location>
            <begin position="22"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005042850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="98" mass="10944" checksum="97F7E35141B99183" modified="2023-09-13" version="1">MRLSLPVLLVTLALCYYEADAVICPAALTDATQFLFISEALYKLQLQRFNAPPEAVEAKLQVKRCTDKISLGNRVLLTKAWVKVILKCGYIDLSKILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J3V8</accession>
    <name>A0A9L0J3V8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Transmembrane protein 132D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TMEM132D</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM132 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J3V8"/>
    <dbReference type="Ensembl" id="ENSEAST00005066817.1">
        <property type="protein sequence ID" value="ENSEASP00005047956.1"/>
        <property type="gene ID" value="ENSEASG00005013187.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158942"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055422">
        <property type="entry name" value="Ig_TMEM132_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031437">
        <property type="entry name" value="Ig_TMEM132_4th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055423">
        <property type="entry name" value="Ig_TMEM132_5th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055424">
        <property type="entry name" value="Ig_TMEM132_6th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026307">
        <property type="entry name" value="TMEM132"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055421">
        <property type="entry name" value="TMEM132_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031436">
        <property type="entry name" value="TMEM132_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031435">
        <property type="entry name" value="TMEM132_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13388">
        <property type="entry name" value="DETONATOR, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13388:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 132D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23481">
        <property type="entry name" value="Ig_TMEM132_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16070">
        <property type="entry name" value="Ig_TMEM132_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23486">
        <property type="entry name" value="Ig_TMEM132_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23487">
        <property type="entry name" value="Ig_TMEM132_6th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23039">
        <property type="entry name" value="TMEM132_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15706">
        <property type="entry name" value="TMEM132_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15705">
        <property type="entry name" value="TMEM132_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040492113" evidence="8">
        <location>
            <begin position="31"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="918"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 N-terminal" evidence="9">
        <location>
            <begin position="49"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 second Ig-like" evidence="13">
        <location>
            <begin position="128"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 cohesin-like" evidence="12">
        <location>
            <begin position="281"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein family 132 fourth" evidence="11">
        <location>
            <begin position="427"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 fifth" evidence="14">
        <location>
            <begin position="527"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 sixth" evidence="15">
        <location>
            <begin position="664"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="domain" description="Transmembrane protein TMEM132 C-terminal" evidence="10">
        <location>
            <begin position="887"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="238"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="799"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1013"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="834"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="854"/>
            <end position="865"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF15705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF15706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
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</entry>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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                <person name="Wang"/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
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                <person name="J."/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
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        <subcellularLocation>
            <location evidence="10">Endomembrane system</location>
            <topology evidence="10">Single-pass type I membrane protein</topology>
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    <comment type="similarity">
        <text evidence="2">Belongs to the immunoglobulin superfamily. LISCH7 family.</text>
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        <property type="component" value="Chromosome 26"/>
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        <property type="entry name" value="Ig-like_domain-LISCH7"/>
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        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
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    <dbReference type="InterPro" id="IPR008664">
        <property type="entry name" value="LISCH7"/>
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    <dbReference type="PANTHER" id="PTHR15923:SF1">
        <property type="entry name" value="LIPOLYSIS-STIMULATED LIPOPROTEIN RECEPTOR"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR15923">
        <property type="entry name" value="TRANSMEMBRANE AND IMMUNOGLOBULIN DOMAIN-CONTAINING PROTEIN"/>
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        <property type="entry name" value="LSR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
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            <begin position="88"/>
            <end position="236"/>
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            <begin position="1"/>
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        <location>
            <begin position="410"/>
            <end position="646"/>
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            <begin position="1"/>
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</entry>
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            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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                <person name="Guo"/>
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                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Fang"/>
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                <person name="Wang"/>
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                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
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                <person name="Ju"/>
                <person name="Z."/>
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                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <source>
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</entry>
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    <accession>A0A9L0JA86</accession>
    <name>A0A9L0JA86_EQUAS</name>
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            <fullName evidence="10">DNA-directed RNA polymerases I, II, and III subunit RPABC4</fullName>
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            <fullName evidence="11">DNA-directed RNA polymerase II subunit K</fullName>
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        <name type="scientific">Equus asinus</name>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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                <consortium name="Ensembl"/>
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    <comment type="function">
        <text evidence="9">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the archaeal Rpo12/eukaryotic RPC10 RNA polymerase subunit family.</text>
    </comment>
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="RPABC4/Spt4"/>
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        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III"/>
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        <property type="entry name" value="RNA POLYMERASE II, I AND III SUBUNIT K"/>
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        <property type="entry name" value="Zn_ribbon_RPAB4"/>
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    </dbReference>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSEAST00005051679.1">
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007959"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR038818">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37856:SF1">
        <property type="entry name" value="MAST CELL-EXPRESSED MEMBRANE PROTEIN 1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
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        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
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        <location>
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    </evidence>
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        <source>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
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                <person name="Guo"/>
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                <person name="Sun"/>
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                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Liu"/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
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                <person name="A."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
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            </authorList>
        </citation>
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        <text evidence="10">Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends. Without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Promotes binding of POT1 to single-stranded telomeric DNA. Modulates the inhibitory effects of POT1 on telomere elongation. The ACD-POT1 heterodimer enhances telomere elongation by recruiting telomerase to telomeres and increasing its processivity. May play a role in organogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP ACD and POT1. Forms heterodimers with POT1. Identified in a complex with POT1 and single-stranded telomeric DNA. Interacts with STN1 and TINF2.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0779">Telomere</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Shelterin complex subunit TPP1/Est3" evidence="13">
        <location>
            <begin position="9"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="377"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    <accession>A0A9L0JJH4</accession>
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            <fullName evidence="3">Fibroblast growth factor</fullName>
            <shortName evidence="3">FGF</shortName>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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    <comment type="similarity">
        <text evidence="1 3">Belongs to the heparin-binding growth factors family.</text>
    </comment>
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        <location>
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        <location>
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            <begin position="215"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00007936"/>
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        <source>
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    </evidence>
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        <source>
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    <sequence length="245" mass="27588" checksum="5B96D41AC3A3DF78" modified="2023-09-13" version="1">MAAAIASSLIRQKRQAREREKSNACKCVSSPSKGKTSCDKNKLNVFSRVKLFGSKKRRRRRPEPQLKGIVTKLYSRQGYHLQLQADGTIDGTKDEDSTYTLFNLIPVGLRVVAIQGVQTKLYLAMNSEGYLYTSEHFTPECKFKESVFENYYVTYSSMIYRQQQSGRGWYLGLNKEGEIMKGNHVKKNKPAAHFLPKPLKVAMYKEPSLHDLTEFSRSGSGTPTKSRSVSGVLNGGKSMSHNEST</sequence>
</entry>
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    <accession>A0A9L0JMK7</accession>
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        <submittedName>
            <fullName evidence="11">Divergent protein kinase domain 1B</fullName>
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        <name evidence="11" type="primary">DIPK1B</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
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    <comment type="similarity">
        <text evidence="2">Belongs to the DIPK family.</text>
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        <property type="entry name" value="FAM69_N"/>
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        <property type="entry name" value="DIVERGENT PROTEIN KINASE DOMAIN 1B"/>
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        <property type="entry name" value="DIVERGENT PROTEIN KINASE DOMAIN 1C-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="PIP49_C"/>
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        <property type="entry name" value="PIP49_N"/>
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    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
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    <feature type="domain" description="FAM69 N-terminal" evidence="10">
        <location>
            <begin position="22"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006338"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM01299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005051217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="431" mass="48255" checksum="AE750DB54B8120DC" modified="2023-09-13" version="1">MRRLRRLAHLVLFCPFSKGLQGRLPGLRVKCVFLVWLGVFAGSWLAYMHYSSYAELCRGHICQVVICDQYHKGIISGSICQDLCNLHKVEWRTCLSSVPGQQVYSGLWQGKEVTIKCGIEESLNSKAGSDSAPRRELVLFDKPTRGTSIKEFREMTLGFLKANLGDLPSLPALVGQVLLMADFNQDSRVSLAEAKSMWALLQRNEFLLLLSLQEKEHASRLLGYCGDLYVTEGAPHGSWHGAALPPLLRPLLPPALHSALQQWLGPAWPWRAKIAIGLLEFVEELFHGSYGTFYMCETTLANVGYTAKYDFKMADLQQVAPEAAVRRFLQGRHCEHSSDCTYGRDCRAPCDKLMRQCKGDLIQPNLAKVCELLRDYLLPGAPADLHAELGRQLRTCTTLSGLASQVEAHHSLVLSHLKTLLWKKISNTKYS</sequence>
</entry>
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    <accession>A0A9L0JSV3</accession>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
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                <person name="S."/>
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                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="component" value="Chromosome 13"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11210:FF:000003">
        <property type="entry name" value="RAP1 GTPase activating protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11210">
        <property type="entry name" value="Rap/Ran-GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035974">
        <property type="entry name" value="Rap/Ran-GAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000331">
        <property type="entry name" value="Rap/Ran_GAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050989">
        <property type="entry name" value="Rap1_Ran_GAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711">
        <property type="entry name" value="RAP GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15711:SF17">
        <property type="entry name" value="RAP1 GTPASE-ACTIVATING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21022">
        <property type="entry name" value="Rap-GAP_dimer"/>
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        <property type="entry name" value="RAPGAP"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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            <begin position="227"/>
            <end position="443"/>
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            <begin position="9"/>
            <end position="32"/>
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            <begin position="487"/>
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            <begin position="528"/>
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            <begin position="564"/>
            <end position="592"/>
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            <begin position="597"/>
            <end position="610"/>
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            <begin position="615"/>
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            <begin position="679"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00057316"/>
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    <evidence type="ECO:0000256" key="3">
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            <dbReference type="PROSITE-ProRule" id="PRU00165"/>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="710" mass="78155" checksum="B3A49982684C0CAE" modified="2023-09-13" version="1">MPRKIWKQELANSSDVTLPDRPLSPPLTAPPTMKSAEFFEMLEKMQGIKLEEQKPGPQKNKDDYIPYPSIDEIVEKGGPYPLIVLPQFGGYWIEDPENLGTPTSLGSSICEEEEEDSLSPNTFGYKLECRGEARAYRRHFLGKDHLNFYCTGSSLGNLLLSIKCEEAEGIEYLRIILRSKVKTVHERIPLAGLSKLPSVPQIAKAFCDDAVGLKFNPVLYPKASQMIVSYDEHDVNNTFKFGVIYQKARQTLEEELFGNNEESPAFKEFLDLLGDTIMLQDFKGFRGGLDVTHGQTGVESVYTIFRDREIMFHVSTKLPFTEGDAQQLQRKRHIGNDIVAIIFQEENTPFVPDMIASNFLHAYIVVQAENAGTETPSYKVSVTAREDVPAFGPPLPSPPVFQKGPEFREFLLTKLTNAENACCKSDKFAKLEDRTRAALLDNLHDELHAHTQAMLGLGPEEDKFENGGHGGFLESFKRAIRVRSHSMETMVSSQKKQHSGGIPGSLSGGISHNSVEVTKTTFSPPVAVATAKNQSRSPIKRRSGLFPRLHTGSEGQGDSRTRSDSASSNPKTPDGGHSSQEIKSETSSNPSSPEICPNKEKPFIKLKENGRANISRSSSSTSSFSSTAGEGEAMEECDSGSSQLSTTSPFKQEVFVYSQSPSSESPSLGAAATPIIMSRSPTDVKSRNSPRSNLKFRFDKLSHAGSSAGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JUY9</accession>
    <name>A0A9L0JUY9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Amino acid transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SLC1A2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>D-aspartate(out) + K(+)(in) + 3 Na(+)(out) + H(+)(out) = D-aspartate(in) + K(+)(out) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:71379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29990"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>K(+)(in) + L-aspartate(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-aspartate(in) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70851"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>K(+)(in) + L-glutamate(out) + 3 Na(+)(out) + H(+)(out) = K(+)(out) + L-glutamate(in) + 3 Na(+)(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:70699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JUY9"/>
    <dbReference type="Ensembl" id="ENSEAST00005065528.1">
        <property type="protein sequence ID" value="ENSEASP00005052955.1"/>
        <property type="gene ID" value="ENSEASG00005020253.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155379"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015501">
        <property type="term" value="F:glutamate:sodium symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0015175">
        <property type="term" value="F:neutral L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070778">
        <property type="term" value="P:L-aspartate transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098712">
        <property type="term" value="P:L-glutamate import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070633">
        <property type="term" value="P:transepithelial transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150104">
        <property type="term" value="P:transport across blood-brain barrier"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Amino acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3860.10">
        <property type="entry name" value="Sodium:dicarboxylate symporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050746">
        <property type="entry name" value="DAACS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001991">
        <property type="entry name" value="Na-dicarboxylate_symporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018107">
        <property type="entry name" value="Na-dicarboxylate_symporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036458">
        <property type="entry name" value="Na:dicarbo_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958:SF93">
        <property type="entry name" value="EXCITATORY AMINO ACID TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11958">
        <property type="entry name" value="SODIUM/DICARBOXYLATE SYMPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00375">
        <property type="entry name" value="SDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00173">
        <property type="entry name" value="EDTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118215">
        <property type="entry name" value="Proton glutamate symport protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00713">
        <property type="entry name" value="NA_DICARBOXYL_SYMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00714">
        <property type="entry name" value="NA_DICARBOXYL_SYMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0769">Symport</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="88"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="239"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="276"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="313"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="345"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="433"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="574" mass="62151" checksum="5CC0E638678AD93C" modified="2023-09-13" version="1">MASTEGANNMPKQVEVRMHDSHLSAEEPRHQHLGLRLCDKLRKNLLLTLTVFGVVLGAVCGGLLRLASPIHPDVVMLIAFPGDILMRMLKMLILPLILSSLITGLSGLDAKASGRLGTRAMVYYMSTTIIAAVLGVILVLAIHPGNPKLKKQLGPGKKNDEVSSLDAFLDLIRNLFPENLVQACFQQIQTVTKKVLVAPPADEEGNATNAVISLLNETVTETPEETNVVIKKGLEFKDGMNVLGLIGFFIAFGIAMGKMGEQAKLMVEFFNILNEIVMKLVIMIMWYSPLGIACLICGKIIAIKDLEVVARQLGMYMVTVIVGLIIHGGIFLPLIYFLVTRKNPFSFFAGIFQAWITALGTASSAGTLPVTFRCLEENLGIDKRVTRFVLPVGATINMDGTALYEAVAAIFIAQMNGVVLDGGQIVTVSLTATLASVGAASIPSAGLVTMLLILTAVGLPTEDISLLVAVDWLLDRMRTSVNVVGDSFGAGIVYHLSKSELDTIDSQHRMHEDIEMTKTQSIYDDMKNHRESNSSQCVYAAHNSVIVDECKVTLAANGKSADCSVEDEPWKQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JV20</accession>
    <name>A0A9L0JV20_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Elongation of very long chain fatty acids protein 6</fullName>
            <ecNumber evidence="19">2.3.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">3-keto acyl-CoA synthase ELOVL6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">ELOVL fatty acid elongase 6</fullName>
            <shortName evidence="19">ELOVL FA elongase 6</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Very long chain 3-ketoacyl-CoA synthase 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Very long chain 3-oxoacyl-CoA synthase 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19 21" type="primary">ELOVL6</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that elongates fatty acids with 12, 14 and 16 carbons with higher activity toward C16:0 acyl-CoAs. Catalyzes the synthesis of unsaturated C16 long chain fatty acids and, to a lesser extent, C18:0 and those with low desaturation degree. May participate to the production of saturated and monounsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(9Z)-hexadecenoyl-CoA + malonyl-CoA + H(+) = 3-oxo-(11Z)-octadecenoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:39675"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:61540"/>
            <dbReference type="ChEBI" id="CHEBI:76555"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:39676"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(9Z)-octadecenoyl-CoA + malonyl-CoA + H(+) = 3-oxo-(11Z)-eicosenoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:36511"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:74011"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:36512"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(9Z,12Z)-octadecadienoyl-CoA + malonyl-CoA + H(+) = (11Z,14Z)-3-oxoicosa-11,14-dienoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:36503"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57383"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:74012"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:36504"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(9Z,12Z,15Z)-octadecatrienoyl-CoA + malonyl-CoA + H(+) = (11Z,14Z,17Z)-3-oxoeicosatrienoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:36523"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:74034"/>
            <dbReference type="ChEBI" id="CHEBI:74054"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:36524"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 20">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>dodecanoyl-CoA + malonyl-CoA + H(+) = 3-oxotetradecanoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:60140"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:62543"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:60141"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>hexadecanoyl-CoA + malonyl-CoA + H(+) = 3-oxooctadecanoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:35315"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:71407"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:35316"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>tetradecanoyl-CoA + malonyl-CoA + H(+) = 3-oxohexadecanoyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:39167"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57349"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:39168"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="19">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Endoplasmic reticulum membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="19">N-Glycosylated.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the ELO family. ELOVL6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.199" evidence="19"/>
    <dbReference type="RefSeq" id="XP_014694545.1">
        <property type="nucleotide sequence ID" value="XM_014839059.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JV20"/>
    <dbReference type="Ensembl" id="ENSEAST00005071293.1">
        <property type="protein sequence ID" value="ENSEASP00005052985.1"/>
        <property type="gene ID" value="ENSEASG00005004683.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106829791"/>
    <dbReference type="KEGG" id="eai:106829791"/>
    <dbReference type="CTD" id="79071"/>
    <dbReference type="GeneTree" id="ENSGT01050000244965"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
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    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0019367">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035338">
        <property type="term" value="P:long-chain fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
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        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03206">
        <property type="entry name" value="VLCF_elongase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033675">
        <property type="entry name" value="ELOVL6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF125">
        <property type="entry name" value="ELONGATION OF VERY LONG CHAIN FATTY ACIDS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 19" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 19 20" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="6 19 20" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11 19" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2 19 20" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 19 20" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 19 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 19 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 19 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 19 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19 20">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19 20">
        <location>
            <begin position="63"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19 20">
        <location>
            <begin position="139"/>
            <end position="156"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19 20">
        <location>
            <begin position="162"/>
            <end position="183"/>
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    <feature type="transmembrane region" description="Helical" evidence="19 20">
        <location>
            <begin position="195"/>
            <end position="220"/>
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    <feature type="transmembrane region" description="Helical" evidence="19 20">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048148"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00052042"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03206"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052985.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JXH5</accession>
    <name>A0A9L0JXH5_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Secretion regulating guanine nucleotide exchange factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SERGEF</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JXH5"/>
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        <property type="protein sequence ID" value="ENSEASP00005057183.1"/>
        <property type="gene ID" value="ENSEASG00005007572.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160684"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
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        <property type="entry name" value="Secretion-regulating guanine nucleotide exchange factor"/>
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        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
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    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
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    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
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    <dbReference type="InterPro" id="IPR051625">
        <property type="entry name" value="Signaling_Regulatory_Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872">
        <property type="entry name" value="BTK-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
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    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="44"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="RCC1-like" evidence="4">
        <location>
            <begin position="50"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="97"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="149"/>
            <end position="200"/>
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    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="201"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="260"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="313"/>
            <end position="380"/>
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    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="380"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="440"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="452"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="470"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
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    <evidence type="ECO:0000256" key="3">
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        </source>
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    <evidence type="ECO:0000313" key="5">
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
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    <sequence length="491" mass="51669" checksum="7797FEC834F34867" modified="2023-09-13" version="1">RREDSVEGSAGRGPPRLSPVGPPVLHPPFGRRLQALPQPHPCLAGASSDGANSYGQLGLGHKEDVLLPQQLNDFCKPGCVRRITGGGGHSAVVTDGGSLFVCGLNKDGQLGLGHTEDVLYFTPCRSLLGCPIQQVACGWDFTIILTGNGQLLSCGSNSFGQLGVPHGPRRCVVPQAIEPLREKVVSIAAGLRHALAATASGIVFQWGTGLASSGRRLCPGETLPLFLTAKEPSRVTGLEDSKATCVVAGSDHSASLTDAGELYVWGSNKHGQLASQAAFLPVPQKIEAHCFQHEKVTAVWSGWTHLVAQTETGKVFTWGRADYGQLGRKLESHENWKLDKQGLSLPCPRPQKSTPSPLHCLMGATEVSCGSEHNLAVIGGACYSWGWNEHGMCGDGTEANVWAPKPVQSLRLSSGLLVGCGAGHSLALCQLPALPALDQGSKVTGPSPDATEDAKSQEAMDKEKLEGKTTRISTQSQSDTCRNGGLVTEPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JZ65</accession>
    <name>A0A9L0JZ65_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Caveolin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CAV3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress. Mediates the recruitment of CAVIN2 and CAVIN3 proteins to the caveolae.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <location evidence="1">Sarcolemma</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Golgi apparatus membrane</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Membrane</location>
            <location evidence="10">Caveola</location>
            <topology evidence="10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the caveolin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JZ65"/>
    <dbReference type="Ensembl" id="ENSEAST00005045818.1">
        <property type="protein sequence ID" value="ENSEASP00005058556.1"/>
        <property type="gene ID" value="ENSEASG00005033820.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183006"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016010">
        <property type="term" value="C:dystrophin-associated glycoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030315">
        <property type="term" value="C:T-tubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043014">
        <property type="term" value="F:alpha-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005246">
        <property type="term" value="F:calcium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071253">
        <property type="term" value="F:connexin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017080">
        <property type="term" value="F:sodium channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055013">
        <property type="term" value="P:cardiac muscle cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003300">
        <property type="term" value="P:cardiac muscle hypertrophy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070836">
        <property type="term" value="P:caveola assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035995">
        <property type="term" value="P:detection of muscle stretch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051649">
        <property type="term" value="P:establishment of localization in cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042593">
        <property type="term" value="P:glucose homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060347">
        <property type="term" value="P:heart trabecula formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000165">
        <property type="term" value="P:MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007520">
        <property type="term" value="P:myoblast fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051926">
        <property type="term" value="P:negative regulation of calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010614">
        <property type="term" value="P:negative regulation of cardiac muscle hypertrophy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045792">
        <property type="term" value="P:negative regulation of cell size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900826">
        <property type="term" value="P:negative regulation of membrane depolarization during cardiac muscle cell action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060299">
        <property type="term" value="P:negative regulation of sarcomere organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051647">
        <property type="term" value="P:nucleus localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001778">
        <property type="term" value="P:plasma membrane repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010831">
        <property type="term" value="P:positive regulation of myotube differentiation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
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                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ITPRIP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K0S0"/>
    <dbReference type="Ensembl" id="ENSEAST00005039502.1">
        <property type="protein sequence ID" value="ENSEASP00005040005.1"/>
        <property type="gene ID" value="ENSEASG00005026382.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005074471.1">
        <property type="protein sequence ID" value="ENSEASP00005055932.1"/>
        <property type="gene ID" value="ENSEASG00005026382.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244827"/>
    <dbReference type="OMA" id="RVYGGPR"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026250">
        <property type="entry name" value="ITPRIP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046906">
        <property type="entry name" value="Mab-21_HhH/H2TH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024810">
        <property type="entry name" value="MAB21L/cGLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656">
        <property type="entry name" value="CELL FATE DETERMINING PROTEIN MAB21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656:SF9">
        <property type="entry name" value="INOSITOL 1,4,5-TRISPHOSPHATE RECEPTOR-INTERACTING PROTEIN-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20266">
        <property type="entry name" value="Mab-21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02107">
        <property type="entry name" value="INOS145TPRIP"/>
    </dbReference>
    <dbReference type="SMART" id="SM01265">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Mab-21-like HhH/H2TH-like" evidence="8">
        <location>
            <begin position="369"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005055932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="532" mass="58289" checksum="9934610C21A9FEBD" modified="2023-09-13" version="1">MSVHYTLNLRVFWPLVTGLCTALVCLYHVLRGSGGARAEPLDGADGGFPLLKVAILLLLGYILLRCRHAVRQRFLPGSPRLGRHTAFSPRHFREPSLAILLESYYEHEVRLSPHVLGHSKAHVSRIVGELVRAGRARGSPGPIPGGALALAFRGDFIQVGSAYEQHKIRRPDGFDVLVPLRLPPLVALEPRGLGAEPGLSPGFHGCFVCALKAPPGASGAHWLRDCKPFADGFCVDVRGRRHLSAALVLRWFQSHLQRSLATVRYSLEERCRVSLTPGGLEQPPTLHILPCRTDYGCCRLSMAVRLIPAVHLGDSVFLVAPPPPPSPFGPLSELPGGLRAEALWGVNTARQEQKLLSWLQERAPPGACYLKCLQLLKALRDLGARGLDPMAATQWGRILSSYVLKTALLAVLLRKGAAAQGWDEAHLVERLEELVQFLRDCLLRRHTLFHCVLGPGGAAVEVGPLPKVLREAAPVDLLAVFDRHARELAAARLLSTWRRLPQLLRAYGGPRYLARCPPPGSQRTQGFPEDEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K1Q9</accession>
    <name>A0A9L0K1Q9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Keratin-associated protein 12-1-like</fullName>
        </recommendedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K1Q9"/>
    <dbReference type="Ensembl" id="ENSEAST00005082662.1">
        <property type="protein sequence ID" value="ENSEASP00005058703.1"/>
        <property type="gene ID" value="ENSEASG00005033364.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00960000187213"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002494">
        <property type="entry name" value="KAP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13885">
        <property type="entry name" value="Keratin_B2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005058703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="12775" checksum="4172008BA3FE542B" modified="2023-09-13" version="1">MCHTSCSTGCQPACPAPCCPPLVCRSSCRQPAPCVSLLCRPVCGSATSCQSACGCLSPCCPPTCGQASSCSPACCVPSPCQVACCVPVSCRPIVYLAPSCQSSRCCQPSCPTLVCRPVLSCSPSCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K5W7</accession>
    <name>A0A9L0K5W7_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">MGAT4 family member C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">MGAT4C</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein glycosylation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044637123.1">
        <property type="nucleotide sequence ID" value="XM_044781188.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044637130.1">
        <property type="nucleotide sequence ID" value="XM_044781195.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070363119.1">
        <property type="nucleotide sequence ID" value="XM_070507018.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070363120.1">
        <property type="nucleotide sequence ID" value="XM_070507019.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070363121.1">
        <property type="nucleotide sequence ID" value="XM_070507020.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070363122.1">
        <property type="nucleotide sequence ID" value="XM_070507021.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070363123.1">
        <property type="nucleotide sequence ID" value="XM_070507022.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070363124.1">
        <property type="nucleotide sequence ID" value="XM_070507023.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070363125.1">
        <property type="nucleotide sequence ID" value="XM_070507024.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070363126.1">
        <property type="nucleotide sequence ID" value="XM_070507025.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070363127.1">
        <property type="nucleotide sequence ID" value="XM_070507026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K5W7"/>
    <dbReference type="Ensembl" id="ENSEAST00005070941.1">
        <property type="protein sequence ID" value="ENSEASP00005056720.1"/>
        <property type="gene ID" value="ENSEASG00005027905.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106838333"/>
    <dbReference type="CTD" id="25834"/>
    <dbReference type="GeneTree" id="ENSGT00940000155352"/>
    <dbReference type="OMA" id="MTDVEHK"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008375">
        <property type="term" value="F:acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006759">
        <property type="entry name" value="Glyco_transf_54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057279">
        <property type="entry name" value="MGAT4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056576">
        <property type="entry name" value="MGAT4_A/B/C_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062:SF14">
        <property type="entry name" value="ALPHA-1,3-MANNOSYL-GLYCOPROTEIN 4-BETA-N-ACETYLGLUCOSAMINYLTRANSFERASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12062">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYLTRANSFERASE VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04666">
        <property type="entry name" value="MGAT4_cons"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23524">
        <property type="entry name" value="MGAT4A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MGAT4 conserved region" evidence="4">
        <location>
            <begin position="66"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="MGAT4 A/B/C C-terminal" evidence="5">
        <location>
            <begin position="343"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005056720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="478" mass="56264" checksum="7014EFF4D6B5C60F" modified="2023-09-13" version="1">MFKFHQMKYIFETLDKMRCLRKRSTVSFLGVLVIFLLFMNLYIEDSYVLEGDKQFGRETSTHQLNSERYVHTFKDLSNFSGAINVTYRYLAATPLQRKRFLTIGLSSVKRKKGNYLLETIKSIFEQSSYEELKEISVVVHLAEFNSSWREVMVQDITQKFAHHIIAGRLIVIHAPEEYYPILDGLKRNYNDPEDRVRFRSKQNVDYAFLLNFCANTSDYYVMLEDDVRCSKNFLTAIKKVITSLEGTYWVTLEFSKLGYIGKLYHSHDLPRLAHFLLMFYQEMPCDWLLTHFRDLLAQKNVIRFKPSLFQHMGYYSSYKGAENKLKDDDFEEESFDIPDNPPASLYTNMNVFESYEASKAYSSVDEYFWGKPPSTGDIFVIVFENPIVIKKIKVTTGTEDRQNDILHHGALDVGENVVRFKRIRQCVTYVRLGEFKNGNFEMSDMNQKIPFDIHCIRIYVTKTQKEWLIIRSISIWTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KB42</accession>
    <name>A0A9L0KB42_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Tubulin tyrosine ligase like 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TTLL3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-glutamyl-[protein] + glycine + ATP = glycyl-L-glutamyl-[protein] + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:67180"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:17207"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:167890"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:67181"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KB42"/>
    <dbReference type="Ensembl" id="ENSEAST00005038603.1">
        <property type="protein sequence ID" value="ENSEASP00005058490.1"/>
        <property type="gene ID" value="ENSEASG00005014156.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154857"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070736">
        <property type="term" value="F:protein-glycine ligase activity, initiating"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000032">
        <property type="entry name" value="tubulin monoglycylase TTLL3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004344">
        <property type="entry name" value="TTL/TTLL_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051437">
        <property type="entry name" value="TTLL_monoglycylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45870">
        <property type="entry name" value="TUBULIN MONOGLYCYLASE TTLL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45870:SF1">
        <property type="entry name" value="TUBULIN MONOGLYCYLASE TTLL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03133">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51221">
        <property type="entry name" value="TTL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="45"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="213"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="601"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="685"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="219"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="734"/>
            <end position="745"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005058490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="745" mass="84483" checksum="2A9960BF92FC54D3" modified="2023-09-13" version="1">MGRLRSAKIHVERAVKQKKIFMIQGRYPVIRCLLRRRGWVEKKMVRHSGTTLPQPQKDLDSSVVGNSDTTEDEDEDEDETFQPPQLFDFDDLLEFDDLDGTHALMSRMVRNEIPYFIWTTRRDVLDCRFLSKDQMINHYARAGSFTTKVGLCLNLRNLPWFDEADADSFFPRCYRLGAEDDKKAFIEDFWLTAARNVLKLVVKSEWKSYSIQAEEEEASGDKQPKKQDGKPGMVSSEFVDEALCACEEHLSNLAHMDIDKDLEAPMYLSPEGWSLFLQRYYQVVHEGAELRHMDTQVRRCEDILQQLRTVVPQMDMEGDRNIWIVKPGAKSRGRGIMCMDHLEEMLKLVDGNPMMMKDGKWVVQKYIERPLLIFGTKFDLRQWFLVTDWNPLTVWFYRDSYIRFSTQPFSLKNLDNSVHLCNNSIQKHLENSCHRHPLLPSDNMWSSQKFQAHLRDMGAPNAWATVIVPGMKAAVIHALQTSQDTVQGRKASFELYGADFVFGEDFQPWLIEINASPTMAPSTAVTARLCAGVQADTLRVVIDRRLDRNCDTGAFELIYKQPPVEVPQYVGIRLLVEGSAIKKPLAMCHRRTGVRPALPHLLTQQGSGEGKDSGTLTHRSAPRKAAGARSLGHTEKPDSTATTSAPGKGKKGKAKSATALVRPSLPKWDPSSTGMGCIFTMTFASGDRQPHPLNRLPLSLKNPQALGKTISPKYPRGPRQFLPALQAPPNRLDLPPPTAPPKNHW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KEN2</accession>
    <name>A0A9L0KEN2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KEN2"/>
    <dbReference type="Ensembl" id="ENSEAST00005071221.1">
        <property type="protein sequence ID" value="ENSEASP00005060987.1"/>
        <property type="gene ID" value="ENSEASG00005026581.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161272"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01852">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000366">
        <property type="entry name" value="GTPase, IMAP family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045058">
        <property type="entry name" value="GIMA/IAN/Toc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903:SF74">
        <property type="entry name" value="GTPASE IMAP FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10903">
        <property type="entry name" value="GTPASE, IMAP FAMILY MEMBER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51720">
        <property type="entry name" value="G_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="234"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="AIG1-type G" evidence="5">
        <location>
            <begin position="1"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005060987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="264" mass="28563" checksum="105D182CA6289B50" modified="2023-09-13" version="1">MLRLILTGRTGAGKSATGNSILGQRRFLSRLSAAQVTTTCAVGSCRWAGWHLDVIDTPPGPRGRCYLLSASGPHALLLVSQLGRFTAQDQQAARRLMAMFGDGAVARTVLLFTHKEDLAGTSLQDYVRCTDNRALRKLVAECGGRVCAFDNRASGTEREAQVAELMALLVRAHGGAPYTNDVYGLARALGRARPEERLRRVAESVAARVQRRRGRGLLAALWGRGKAPWSRVRLVVAALLGALGLLFLLLARRRADALREVDPG</sequence>
</entry>
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        <recommendedName>
            <fullName evidence="5">Proline rich 23A</fullName>
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        <name evidence="3" type="primary">LOC106838152</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PRR23 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KIN0"/>
    <dbReference type="Ensembl" id="ENSEAST00005073400.1">
        <property type="protein sequence ID" value="ENSEASP00005061495.1"/>
        <property type="gene ID" value="ENSEASG00005035070.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007772"/>
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        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018903">
        <property type="entry name" value="PRR23"/>
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    <dbReference type="PANTHER" id="PTHR31813:SF4">
        <property type="entry name" value="PROLINE-RICH PROTEIN 23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31813">
        <property type="entry name" value="PROLINE-RICH PROTEIN 23B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10630">
        <property type="entry name" value="DUF2476"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="157"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="227"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="254"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="263" mass="27693" checksum="7894E0760786E3E8" modified="2023-09-13" version="1">MGSRLRSPSAYPAPWWGPQPGGPGPAKRRRWEEPAGPEAQPAPSLQDLVGPPAAAALTSVVVLAAGCALQLPLDHVDLLLEPEPTSVLQVSLGDHTLVLVPEALLDAGDQRSGGQAHSPFGLEPAAFPGAPGDDAAVEQEFFCASVPEVAAQVEAYDEDADPEFPPSWAAPAARSVAGLRPSAGRESGPSPHGRVPEPSPWAPTPSPERRSPGPYYHLDFHLLEPFPTSPLQPLPPSPSPGPHARAPRPIGPPRKARRRLFEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KLX6</accession>
    <name>A0A9L0KLX6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phospholipase A-2-activating protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">PLAA</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="J."/>
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                <person name="Zhao"/>
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                <person name="S."/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Wang"/>
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                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Plays a role in protein ubiquitination, sorting and degradation through its association with VCP. Involved in ubiquitin-mediated membrane proteins trafficking to late endosomes in an ESCRT-dependent manner, and hence plays a role in synaptic vesicle recycling. May play a role in macroautophagy, regulating for instance the clearance of damaged lysosomes. Plays a role in cerebellar Purkinje cell development. Positively regulates cytosolic and calcium-independent phospholipase A2 activities in a tumor necrosis factor alpha (TNF-alpha)- or lipopolysaccharide (LPS)-dependent manner, and hence prostaglandin E2 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with ubiquitin. Interacts with UBXN6, VCP and YOD1; may form a complex involved in macroautophagy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat PLAP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KLX6"/>
    <dbReference type="Ensembl" id="ENSEAST00005072108.1">
        <property type="protein sequence ID" value="ENSEASP00005062951.1"/>
        <property type="gene ID" value="ENSEASG00005004682.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074944"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000153">
        <property type="term" value="C:cytoplasmic ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016005">
        <property type="term" value="F:phospholipase A2 activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071222">
        <property type="term" value="P:cellular response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900045">
        <property type="term" value="P:negative regulation of protein K63-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903861">
        <property type="term" value="P:positive regulation of dendrite extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001224">
        <property type="term" value="P:positive regulation of neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031394">
        <property type="term" value="P:positive regulation of prostaglandin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903423">
        <property type="term" value="P:positive regulation of synaptic vesicle recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010992">
        <property type="term" value="P:ubiquitin recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043162">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000175">
        <property type="entry name" value="Phospholipase A-2-activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000163">
        <property type="entry name" value="Phospholipase A-2-activating protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.870:FF:000001">
        <property type="entry name" value="Phospholipase A-2-activating protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.870">
        <property type="entry name" value="PFU (PLAA family ubiquitin binding), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015155">
        <property type="entry name" value="PFU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038122">
        <property type="entry name" value="PFU_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013535">
        <property type="entry name" value="PUL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF0">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09070">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08324">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51394">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51396">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0770">Synapse</keyword>
    <keyword evidence="6 15" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="175"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="214"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="15">
        <location>
            <begin position="294"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="PFU" evidence="17">
        <location>
            <begin position="433"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="PUL" evidence="18">
        <location>
            <begin position="601"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="16"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00065331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005062951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="863" mass="94025" checksum="7F1FC462AF28AE8D" modified="2023-09-13" version="1">MSGLLCHSARSARPFPTALPHRLSGSSGLRPPRTGRALSRRGPGHARPPLRTDLARCRAPGAQPTRAMAGGAARYRLSCSLPGHELDVRGLVCCLYPPGAFVSVSRDRTTRLWVPDSPNRGFTEMHCMSGHSNFVSCVCIIPSSDTYPHGLIATGGNDHNICVFSLESPAPLYILKGHKNTVCSLSSGKFGTLLSGSWDTTAKVWLNDKCMMTLQGHTAAVWAVKILPEQGLMLTGSADKTIKLWKAGRCERTFSGHEDCVRGLAILSETEFLSCANDASIRRWQITGECLEVYYGHTNYIYSISVFPNCKDFVTTAEDRSLRIWKRGECAQTIRLPAQSIWCCCVLDNGDIVVGASDGIIRVFTESEDRTASAEEIKAFEKELSQATIDSKTGDLGDINAEQLPGREHLNEPGTREGQTRLIRDGEKVEAYQWSVSEGRWIKIGDVVGSSGATQQTSGKVLYEGKEFDYVFSIDVNEGGPSYKLPYNITDDPWLAAYNFLQKNDLNPMFLDQVAKFIIDNTKGQMLGLGNTSFSDPFTGGGRYVPGSSSGSSNMLPAADPFTGGGRYVPGSAGTGTAMTGVDPFTGNSAYRSASSKTVNIYFPKKEAVTFDQANPTQILGKLKELNGTASEEKKLTEDDLILLEKILSLICNSSSEKPTAQQLQILWKAINWPEDIVFPALDILRLSIKHPSVNENFCNEKEGAQFSSHLISLLNPKGKPANQLLALRTFCNCFVGQAGQKLMMSQRESLMSHAIELKSGSNKNIHIALATLTLNYSVCFHKDHNIEGKAQCLSVISTVLEVVQDLEATFRLLVALGTLISDDSNAVQLAKSLGVDSQIKKYASVSEPAKVSECCRLILNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KVS1</accession>
    <name>A0A8C4KVS1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Corticotropin-releasing factor-binding protein</fullName>
            <shortName evidence="10">CRF-BP</shortName>
            <shortName evidence="10">CRF-binding protein</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Corticotropin-releasing hormone-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">CRHBP</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 10">Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CRF-binding protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KVS1"/>
    <dbReference type="Ensembl" id="ENSEAST00005002564.2">
        <property type="protein sequence ID" value="ENSEASP00005002327.1"/>
        <property type="gene ID" value="ENSEASG00005001822.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001362"/>
    <dbReference type="OMA" id="EFCFPSI"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051424">
        <property type="term" value="F:corticotropin-releasing hormone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048149">
        <property type="term" value="P:behavioral response to ethanol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071320">
        <property type="term" value="P:cellular response to cAMP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071392">
        <property type="term" value="P:cellular response to estradiol stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071391">
        <property type="term" value="P:cellular response to estrogen stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097211">
        <property type="term" value="P:cellular response to gonadotropin-releasing hormone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035865">
        <property type="term" value="P:cellular response to potassium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071356">
        <property type="term" value="P:cellular response to tumor necrosis factor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071466">
        <property type="term" value="P:cellular response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007565">
        <property type="term" value="P:female pregnancy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042445">
        <property type="term" value="P:hormone metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009755">
        <property type="term" value="P:hormone-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002125">
        <property type="term" value="P:maternal aggressive behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051460">
        <property type="term" value="P:negative regulation of corticotropin secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045055">
        <property type="term" value="P:regulated exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080135">
        <property type="term" value="P:regulation of cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001963">
        <property type="term" value="P:synaptic transmission, dopaminergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008435">
        <property type="entry name" value="CRF-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056178">
        <property type="entry name" value="CRF-BP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056177">
        <property type="entry name" value="CRF-BP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10278">
        <property type="entry name" value="CORTICOTROPIN-RELEASING FACTOR-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10278:SF0">
        <property type="entry name" value="CORTICOTROPIN-RELEASING FACTOR-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23541">
        <property type="entry name" value="CRF-BP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05428">
        <property type="entry name" value="CRF-BP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009279">
        <property type="entry name" value="CRF_bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034144473" description="Corticotropin-releasing factor-binding protein" evidence="12">
        <location>
            <begin position="25"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Corticotropin-releasing factor binding protein N-terminal" evidence="13">
        <location>
            <begin position="53"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Corticotropin-releasing factor binding protein C-terminal" evidence="14">
        <location>
            <begin position="185"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="60"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="104"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="237"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="277"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00024997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR009279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR009279-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF05428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005002327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="321" mass="36050" checksum="3E653614F6C3D2D7" modified="2022-01-19" version="1">MSPSFKLQCHFILIFLMALRGDSRYPELREAAHHDPFLLLSASLKRELAGEPLYRRALRCLDMLSLQGQFTFTADRPQLHCAAFFIGEPEEFITIHYDLVSIDCQGGDFLQVFDGWILKGEKFPSSQDHPLPTTERYIDFCDSGLSRRSIRSSQNVAMIFFRVHEPGNGFTLTIKTDPNLFPCNVISQTPNGRFTLVVPHQHRNCSFSIIYPVAIKISDLTLGHLNGLQLKKPSAGCGGKGDFVELLGGTGLDPSKMMPLADLCYPFRGPAQMKIGCDNTVVRMVSSGKHINRVTFEYRQLEPYELENPNGNSIPEFCLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KWH3</accession>
    <name>A0A8C4KWH3_EQUAS</name>
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        <submittedName>
            <fullName evidence="3">Docking protein 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">DOK6</name>
    </gene>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
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                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the DOK family. Type B subfamily.</text>
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        <property type="nucleotide sequence ID" value="XM_044774853.2"/>
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        <property type="gene ID" value="ENSEASG00005000117.2"/>
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        <property type="component" value="Chromosome 7"/>
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        <property type="project" value="TreeGrafter"/>
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        <location>
            <begin position="290"/>
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        </location>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Zhao"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR23166">
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        <property type="match status" value="1"/>
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        </location>
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        <location>
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    <feature type="region of interest" description="Disordered" evidence="3">
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
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        <location>
            <begin position="628"/>
            <end position="774"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="3">
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            <begin position="1"/>
            <end position="15"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
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            <begin position="31"/>
            <end position="47"/>
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            <end position="70"/>
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            <begin position="958"/>
            <end position="972"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1127"/>
            <end position="1138"/>
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        <location>
            <begin position="1139"/>
            <end position="1155"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="4">
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            <dbReference type="Pfam" id="PF09727"/>
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    <evidence type="ECO:0000313" key="5">
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            <dbReference type="Ensembl" id="ENSEASP00005003921.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="1176" mass="134701" checksum="1CDCEA14E638F4B1" modified="2023-09-13" version="1">MRSRNQGGDSSSNGHISCPKPSIISDAGKSLSEDAKEKNKSNRKEEDVMASGTVKRHQKPCGESERKNKKSLELSKEDLIQLLSIMEGELQAREDVIHMLKTEKTKPEVLEAHYGSAEPEKVLRVLHRDAILAQEKSIGEDVYEKPISELDRLEEKQKETYRRMLEQLLLAEKCHRRTVYELENEKHKHTDYMNKSDDFTSLLEQERERLKKLLEQEKAYQARKEKENAKRLNKLRDELVKLKSFALMLVDERQMHIEQLGLQSQKVQDLTQKLREEEEKLKAVTSKSKEDRQRLLRLEADFEHKASRFSQEHEEMNAKLANQESHNRQLRLKLVGLTQRIEELEETNKNLQKAEEELQELREKIAKGECGNSSLMAEVENLRKRVLEMEGKDEEITKTESQCRELRKKLQEEEHHSRELKLEVEKLQKRMSELEKLEEAFSKSKSECTQLHLNLEKEKNLTKDLLNELEVVKSRVKELECSESRLEKAELSLKDDLTKLKSFTVMLVDERKNMMEKIKQEERKVDGLNKNFKVEQGKVMDVTEKLIEESKKLLKLKSEMEEKVYNLTKERDELIGKLKSEEEKSSELSCSVDLLKKRLDGLGEVEREITRGRSRKGPELICPEDNKIKELTLEIERLKKRLQQLEVVEGDLMKTEDEYDQLEQKFRTEQDKANFLSQQLEEIKHQIAKNKAIEKGEAVSQEAELRHRFRLEEAKSRDLKAEVQALKEKIHELMNKEDQLSQLQVDYSVLQQRFMEEENKNKNMGQEVLNLTKELELSKRYSRALRPSVNGRRMVDIPVTSTGVQTDAVSSEVAEEETPAVFIRKSFQEENHIMSNLRQVGLKKPMERSSVLDRYPPAANELTMRKSWIPWMRKRENGPPMSQEKGPRTNSSPGHPGELVLSPKQGQPLHIRVTPDHENSTATLEITSPTSEEFFSSTTVIPTLGNQKPRITIIPSPNVMSQKPKSGDTTLGTERAMSPVTITTFSREKTPESGRGAFADRPTSPIQIMTVSTSAAPAEIAVSPEAQDMPMGRTVLKVTPEKQTVPTPVRKYNSNANIITTEDNKIHIHLGSQFKRSPGTSADGASPVITVRPVNVTAEKEVSTGTVLRSPRNHLSSRPGASKVTSTITITPATTAPTRGTQSVSGQDGSSQRPTPTRIPMSKESIIIHQLRMNSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L0B5</accession>
    <name>A0A8C4L0B5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Zinc finger and BTB domain containing 41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZBTB41</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="ENSEASG00005003240.2"/>
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        <property type="component" value="Chromosome 30"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="BTB_POZ_ZBTB41"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF34">
        <property type="entry name" value="PR_SET DOMAIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="zf-C2H2"/>
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        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
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            <begin position="89"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
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        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
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    <feature type="domain" description="C2H2-type" evidence="14">
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            <begin position="464"/>
            <end position="491"/>
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    <feature type="domain" description="C2H2-type" evidence="14">
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    <feature type="domain" description="C2H2-type" evidence="14">
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005004306.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="921" mass="106650" checksum="F618A49C5FD9DEFC" modified="2023-09-13" version="2">MKKRRKVTSDLDEKIHLGYHKDSAEGNVAVECDQVAYAHPTERPTPEALHCYQELPPSPDQRKLLSSLQYNKNLLKYLNDDRQKQPSFCDLLIIVEGKEFSAHKVVVAVGSSYFHACLSKNPSTDVVTLDHVTHSVFQHLLEFLYTSEFFVYKYEIPLVLEAAKFLDIIDAVKLLNNENVATFQSELTEKSSPEETLNELTGRLSNNHQCKFCSRHFCYKKSLENHLAKTHRSLLLGKKHGLKMLERSFSTRRSKRNRKCPVKFDDTSDDEPESGDGSDNLNQDNFEKEKSDRNDSEDPGSEYNAEEDELEEEMSDEYSDIEEQSEKDNNEAEEEPEAGDSLGNIHEGLTPVVIQNSNKKILQCPKCDKTFDRIGKYESHTRVHTGEKPFECDICHQRYSTKSNLTVHRKKHSNETEFHKKEHKCPYCNKLHASKKTLAKHVKRFHPENAQEFISIKKTKSESWKCDICKKSFTRRPHLEEHMILHSQDKPFKCTYCEEHFKSRFARLKHQEKFHLGPFPCDICGRQFNDTGNLKRHIECTHGGKRKWTCFICGKSVRERTTLKEHLRIHSGEKPHLCSICGQSFRHGSSYSFDGLQFFFLYRLHLRVHHDDKRYECDECGKTFIRHDHLTKHKKIHSGEKAHQCEECGKCFGRRDHLTVHYKSVHLGEKVWQKYKATFHQCDVCKKIFKGKSSLEMHFRTHSGEKPYKCQICNQSFRIKKTLTKHLVIHSDARPFNCQHCNATFKRKDKLKYHIDHVHGIKSPDDPLSTSEEKLVSLPVEYSSDDKIFQTESKQYMDQPKVYQSEAKTMLQNVSAEVCVPVTLVPVQMPDAQSDLVRHTTTLPPSSHEILSSPPQSTDYPRAADLAFLEKYTLTPQPANIVHPVRPEQMLDPREQSYLGTLLGLDNTTAVQNISNNEHHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L364</accession>
    <name>A0A8C4L364_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Granulocyte colony-stimulating factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CSF3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IL-6 superfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L364"/>
    <dbReference type="Ensembl" id="ENSEAST00005003974.2">
        <property type="protein sequence ID" value="ENSEASP00005003617.2"/>
        <property type="gene ID" value="ENSEASG00005002768.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000017328"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071682">
        <property type="term" value="C:endocytic vesicle lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005130">
        <property type="term" value="F:granulocyte colony-stimulating factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038158">
        <property type="term" value="P:granulocyte colony-stimulating factor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030838">
        <property type="term" value="P:positive regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045639">
        <property type="term" value="P:positive regulation of myeloid cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.10:FF:000021">
        <property type="entry name" value="Granulocyte colony-stimulating factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040117">
        <property type="entry name" value="GCSF/MGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030474">
        <property type="entry name" value="IL-6/GCSF/MGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030473">
        <property type="entry name" value="IL6/GCSF/MGF_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10511">
        <property type="entry name" value="GRANULOCYTE COLONY-STIMULATING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10511:SF2">
        <property type="entry name" value="GRANULOCYTE COLONY-STIMULATING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16647">
        <property type="entry name" value="GCSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001935">
        <property type="entry name" value="IL6_MGF_GCSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00433">
        <property type="entry name" value="IL6GCSFMGF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00126">
        <property type="entry name" value="IL6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00254">
        <property type="entry name" value="INTERLEUKIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0202">Cytokine</keyword>
    <keyword evidence="8 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0339">Growth factor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <keyword evidence="12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040232536" description="Granulocyte colony-stimulating factor" evidence="12">
        <location>
            <begin position="31"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="66"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001935-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003617.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="204" mass="22132" checksum="263375B4A2EABF78" modified="2023-09-13" version="2">SAEPAAQNPMELMVLQLLLWHSALWMVQEATPLGSASSLPQSFLLKCLEQVRKIQADGAALQERLCATHKLCHPQELMLLGHSLGIPQPLLSSCSSQALQLTGCLSQLHSGLLLYQGLLQALAGISPELAPTLDMLQLDVTDFATNIWQQMEDLGVAPAVQPTHGPMPTFASAFQRRAGGVLVASNLQRFLELAYRGLRYLAEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L3E7</accession>
    <name>A0A8C4L3E7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sialate O-acetylesterase</fullName>
            <ecNumber evidence="2">3.1.1.53</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Sialic acid-specific 9-O-acetylesterase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SIAE</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the removal of O-acetyl ester groups from position 9 of the free diacetylated sialate N-acetyl-9-O-acetylneuraminate (Neu5,9Ac2) in the cytosol and of the diacetylated sialate residues of sialylglycoconjugates in the lysosomes. Together with the sialate-O-acetyltransferase they regulate the balance of acetylated sialoglycoconjugates, key players in various processes such as cell-cell interactions, host-pathogen recognition, and tumor antigenicity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-acetyl-9-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22600"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28999"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35418"/>
            <dbReference type="EC" id="3.1.1.53"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:22601"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>an Ac-O-9-sialoglycoconjugate + H2O = a sialoglycoconjugate + acetate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:80763"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:231691"/>
            <dbReference type="ChEBI" id="CHEBI:231692"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:80764"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="520" mass="58623" checksum="A550B61347D646A4" modified="2023-09-13" version="2">MVPLGLVFELVLPLILWADISAGVGFRFASYIHDYMVLQKEPAGAVIWGYGVSGATVTVTLWQDQETIMKKVTSVKAHSNSWMVVLDPMKPGGPYKMMAQQTFGTMNFTLSVHDVLFGDVWLCSGQSNMQMTVSQIFNATSELSNTAAYQSVRILSVSLVEAEQELEDLPEVDLQWSKPTAENLGHGSFQYMSAVCWLFGRYLYDTLQYPIGLLASSWGGTPIEAWSSGRSLKACGVPRFTASDFVAGPSQHSVLWNAMIHPLHNMTLKGVIWYQGESNININRDLYNCTFPALIEDWRQTFHRGSQGQTERFFPFGFVQLSSYWSAATSDDEFPEIRWHQTADFGYVPNLRMPNTFMAVAMDLGDWNSSFGSIHPRDKQTVAYRLHLGARAVAYGEKNLIFQGPLPEKIELLTDKGLLNLTYSQQIHVQRQDKIFEISCCRDQRCKWLPAPMNTFSTQTLDLNISSCHDTVVAVRYAWATWPCEYKQCPLYHPTSTLPAPPFIAFITNQTPGYHSKVMK</sequence>
</entry>
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    <accession>A0A8C4L4K9</accession>
    <name>A0A8C4L4K9_EQUAS</name>
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        <submittedName>
            <fullName evidence="6">Phosphofurin acidic cluster sorting protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PACS2</name>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Metazoa</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
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                <person name="Liu"/>
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                <person name="J."/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
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                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the PACS family.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="Ensembl"/>
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    </dbReference>
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    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="20"/>
            <end position="179"/>
        </location>
    </feature>
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        <location>
            <begin position="473"/>
            <end position="885"/>
        </location>
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            <begin position="181"/>
            <end position="245"/>
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    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="292"/>
            <end position="465"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="687"/>
            <end position="740"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
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            <begin position="687"/>
            <end position="720"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="727"/>
            <end position="737"/>
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    </feature>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
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    </evidence>
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    <accession>A0A8C4L679</accession>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that specifically elongates C24:0 and C26:0 acyl-CoAs. May participate to the production of saturated and monounsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May play a critical role in early brain and skin development.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Oligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The C-terminal di-lysine motif may confer endoplasmic reticulum localization.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family. ELOVL4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.199" evidence="10"/>
    <dbReference type="RefSeq" id="XP_014715246.1">
        <property type="nucleotide sequence ID" value="XM_014859760.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A8C4L679"/>
    <dbReference type="Ensembl" id="ENSEAST00005008112.2">
        <property type="protein sequence ID" value="ENSEASP00005007434.1"/>
        <property type="gene ID" value="ENSEASG00005005437.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106842949"/>
    <dbReference type="KEGG" id="eai:106842949"/>
    <dbReference type="CTD" id="6785"/>
    <dbReference type="GeneTree" id="ENSGT01050000244838"/>
    <dbReference type="OMA" id="WTYFTST"/>
    <dbReference type="OrthoDB" id="434092at2759"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035338">
        <property type="term" value="P:long-chain fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03204">
        <property type="entry name" value="VLCF_elongase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033678">
        <property type="entry name" value="ELOVL4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF12">
        <property type="entry name" value="ELONGATION OF VERY LONG CHAIN FATTY ACIDS PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10 11" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10 11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="189"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="246"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="275"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Di-lysine motif" evidence="10">
        <location>
            <begin position="310"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="293"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_03204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="314" mass="36678" checksum="92295687829EA9FB" modified="2022-01-19" version="1">MGLLDAEPGSVLNVVSTALNDTVELYRWTCSFADKRVENWPLMQSPWTTLGISTLYLLFVWLGPKWMKDREPFQMRLVLIIYNFGMVFLNLFIFRELFMGSYNAGYSYICQTVDYSDNVHEVRIAAALWWYFVSKGVEYLDTVFFILRKKNNQVSFLHVYHHCTMFTLWWIGIKWVAGGQAFFGAQMNSLIHVIMYSYYGLSAFGPWIQKYLWWKRYLTMLQLIQFHVTIGHTALSLYTDCPFPKWMHWALIVYSISFILLFLNFYVRTYNMPKKSQTGKPAVNGISANGVSKSEKQLVMENGKKQKTGKAKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L969</accession>
    <name>A0A8C4L969_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Interleukin-12 receptor subunit beta-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">IL-12 receptor beta component</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">IL12RB1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Also associates with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Dimer or oligomer; disulfide-linked. Interacts with IL12RB2 to form the high affinity IL12 receptor. Heterodimer with IL23R; in presence of IL23. The heterodimer forms the IL23 receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 2 subfamily.</text>
    </comment>
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    <dbReference type="Ensembl" id="ENSEAST00005009032.2">
        <property type="protein sequence ID" value="ENSEASP00005008287.2"/>
        <property type="gene ID" value="ENSEASG00005005987.2"/>
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    <dbReference type="GeneTree" id="ENSGT00390000012431"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
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    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0042022">
        <property type="term" value="C:interleukin-12 receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072536">
        <property type="term" value="C:interleukin-23 receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015026">
        <property type="term" value="F:coreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016517">
        <property type="term" value="F:interleukin-12 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005143">
        <property type="term" value="F:interleukin-12 receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042019">
        <property type="term" value="F:interleukin-23 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042020">
        <property type="term" value="F:interleukin-23 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071346">
        <property type="term" value="P:cellular response to type II interferon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042104">
        <property type="term" value="P:positive regulation of activated T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002230">
        <property type="term" value="P:positive regulation of defense response to virus by host"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002827">
        <property type="term" value="P:positive regulation of T-helper 1 type immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032729">
        <property type="term" value="P:positive regulation of type II interferon production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045063">
        <property type="term" value="P:T-helper 1 cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001613">
        <property type="entry name" value="Interleukin 12 receptor subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001717">
        <property type="entry name" value="Interleukin 12 receptor subunit beta 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23037:SF35">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 16" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040305499" description="Interleukin-12 receptor subunit beta-1" evidence="16">
        <location>
            <begin position="23"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="17">
        <location>
            <begin position="450"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="628"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="663"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00067634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008287.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="709" mass="76957" checksum="FAF0FFE8C76C6139" modified="2023-09-13" version="2">MGQPVARLVPVLLLLLPKRGAGSCSSSGCCFQDPPYPDADSGSTSGPRDLNCYRTSSAGYECSWRYEGPAAGVSHFLRCCLRPGRCCYFAAGSATTLQFSDQDGISVLYNVTLWVESRTANRTEKSPEVTLQLYSSVKYDPPPGDIKVSRVAGQLRMEWEMPTRQGGAEVQFRHRTPGSSWKLGDCGLQDDAGFESCLCPMETDAVQEFQLRRRRRPEAPGGLWSSWSSPVCVPPENPPEPEVRFSVKQLGHNGRRQVTLEGQPPPLELPEGCRGPAAGVQVAYRVRLRMLSCPCQPHAARTLRLPRTLLLSGAAYDLAVVSRTRLGRGPNRTWHIPAAERPEPGALSISAGAHGTTLRWPARAQGTTYCIEWQPQGQDGSLTTCTLTAPEDQDPAEMATHSWSWASAVMAQRACYYITIFASERPEKPTSWSTVLSTYHFGGNASEAGSPQRVSVKQLGRDSVSVAWTPSLLSTCPGVLKEYVVRCWEEGSGPGSELPVKPTETRATLGGLRAGAAYTVQVRADTETRRGAWSPPHRFSVEAQVSELSVVLVSLGSFVSVLLLGTLGYLGLSRAIRHLCPPLPTPCASTAVELPHSQGKQAWQWISPADFPEESSPEKVLVVSMPCDKHEETDLDTPEPLEEKPKPPRGAPEPALDTELPSEDGRQVQEHLRGRRPGAWRAGRSGGQPCPGGWTPSAPGRSNAEPQVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L9W2</accession>
    <name>A0A8C4L9W2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Oxysterol-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">OSBPL10</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Probable lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane. Its ability to bind phosphatidylserine, suggests that it specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P. Plays a role in negative regulation of lipid biosynthesis. Negatively regulates APOB secretion from hepatocytes. Binds cholesterol and acidic phospholipids. Also binds 25-hydroxycholesterol. Binds phosphatidylserine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with OSBPL9. Interacts with DIAPH1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014682657.1">
        <property type="nucleotide sequence ID" value="XM_014827171.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L9W2"/>
    <dbReference type="Ensembl" id="ENSEAST00005009364.2">
        <property type="protein sequence ID" value="ENSEASP00005008588.2"/>
        <property type="gene ID" value="ENSEASG00005006177.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822131"/>
    <dbReference type="KEGG" id="eai:106822131"/>
    <dbReference type="CTD" id="114884"/>
    <dbReference type="GeneTree" id="ENSGT00940000157880"/>
    <dbReference type="OrthoDB" id="48057at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015485">
        <property type="term" value="F:cholesterol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd13291">
        <property type="entry name" value="PH_ORP10_ORP11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.2720:FF:000001">
        <property type="entry name" value="Oxysterol-binding OBPalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000278">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000002">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3490:FF:000001">
        <property type="entry name" value="Oxysterol-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041680">
        <property type="entry name" value="PH_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF47">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15409">
        <property type="entry name" value="PH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 16" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="11" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PH" evidence="18">
        <location>
            <begin position="74"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="304"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="356"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="498"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="15"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="374"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="509"/>
            <end position="519"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008588.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="763" mass="83845" checksum="83BED523EDAC90C7" modified="2023-09-13" version="2">MERAAQGADGGGGSNSSSRSSSRATSAGSSPSCSLAGRGVSSRSAAAGLGGGGSRSSPGSVAASPSGGGGRRREPALEGVLSKYTNLLQGWQNRYFVLDFEAGILQYFVNEQSKHQKPRGALSLSGAIVSLSDEAPHMLVVYSANGEMYKLRAADAKEKQFWVTQLRACAKYHMETNSKTTPSSRSRSLTLLPHGTPNSASPCSQRHLGAGAPGVVTITHHKSPAAARRVKSQYSGQLHEVREMMNQVEGQQKNLVHAIESLPGSGPLTALDQDLLLLKATSAATLSCLGECLSLLQQSVHHVSQPSHKPGASENILGWHGPKSHSTEQLKNGTLGSLPSASANITWAILPNSAEDEQTLQPEPEPNLGPELVLSEDEKSDTEDKEETELGVMEDQRSVILHLISQLKLGMDLTKVVLPTFILEKRSLLEMYADFMAHPDLLLAITAGATPEERVICFVEYYLTAFHEGRKGALAKKPYNPIIGETFHCSWEVPKDRVKPKRTAPHSPASHEHPMADDPSKSYKLRFVAEQVSHHPPISCFYCECKEKRLCVNTHVWTKSKFMGMSVGVSMIGEGVLRLLEHGEEYVFTLPSAYARSILTIPWVELGGKVSISCAKTGYSATVTFHTKPFYGGKVHRVTAEVKHNPTNTIVCKAHGEWNGTLEFTYNNGETKVIDTTTLPVYPKKIRPLEKQGPMESRNLWREVTRYLRLGDIDAATEQKRRLEEKQRVEERKRENLRTPWQPKYFIQEGDGWVYFNPLWKAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LAR6</accession>
    <name>A0A8C4LAR6_EQUAS</name>
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        <recommendedName>
            <fullName evidence="4">Dynein light chain</fullName>
        </recommendedName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LAR6"/>
    <dbReference type="Ensembl" id="ENSEAST00005006984.2">
        <property type="protein sequence ID" value="ENSEASP00005006386.2"/>
        <property type="gene ID" value="ENSEASG00005004721.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001618"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48424">
        <property type="entry name" value="DYNEIN LIGHT CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48424:SF3">
        <property type="entry name" value="DYNEIN LIGHT CHAIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006386.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="140" mass="15253" checksum="AD5AEA871081DED1" modified="2023-09-13" version="2">MLLTRFEQQNGHLAQVEVDEVFGFMCHVTTEVPPHNAVPGGVVLLVKLLGVGKKKNTRILRLSSTLHRVLLHLLRHVGIFDHGLSVAHGYLQGSWGWPCGLVVKFPCSTATAQGFTGSDPGLRRGTAGQATLRQRPTCHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LAS4</accession>
    <name>A0A8C4LAS4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial</fullName>
            <shortName evidence="1">Glu-AdT subunit C</shortName>
            <ecNumber evidence="1">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1 2" type="primary">GATC</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (GATB) and C (GATC) subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GatC family.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="1"/>
    <dbReference type="RefSeq" id="XP_014717945.1">
        <property type="nucleotide sequence ID" value="XM_014862459.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LAS4"/>
    <dbReference type="SMR" id="A0A8C4LAS4"/>
    <dbReference type="Ensembl" id="ENSEAST00005006996.2">
        <property type="protein sequence ID" value="ENSEASP00005006398.2"/>
        <property type="gene ID" value="ENSEASG00005004729.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106844792"/>
    <dbReference type="CTD" id="283459"/>
    <dbReference type="GeneTree" id="ENSGT00390000018351"/>
    <dbReference type="OrthoDB" id="5394539at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00122">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="GatC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00135">
        <property type="entry name" value="gatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15004:SF0">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT C, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02686">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="Glu-tRNAGln amidotransferase C subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006398.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="136" mass="15176" checksum="05777B82BB3B26DD" modified="2023-09-13" version="2">MWARAWRLGLRALVGGRRGFTCKADTKGSGRITAEVIEHLERLALVDFGSQEAVARLEKAIAFADRLRAVDTDGVEPMESVLEDRCLYLRSDHVIEGNCAEELLQNSHRVVEEYFVAPPGNISLPKLDEQEPFSHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LBR6</accession>
    <name>A0A8C4LBR6_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Leucine rich repeat containing 25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LRRC25</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LBR6"/>
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        <property type="protein sequence ID" value="ENSEASP00005008695.2"/>
        <property type="gene ID" value="ENSEASG00005006241.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004001"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:endoplasmic reticulum"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
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    <dbReference type="InterPro" id="IPR039243">
        <property type="entry name" value="LRRC25"/>
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    <dbReference type="PANTHER" id="PTHR20878">
        <property type="entry name" value="LEUCINE-RICH REPEAT CONTAINING PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20878:SF0">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="215"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="35"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008695.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="352" mass="38345" checksum="2E50B78327775962" modified="2023-09-13" version="2">LPLCTPPGHQFLFPPSRWVQSLRDGREHSRSDPRRVPAAPAPASRLPEWGAPWRGCCCCCLLLQDPGSQGLSCNVSSGGVDWTKAFTDTCLNFSGQGLSLPQNQFLQASSVAILDLSGNSLRELPLPFFTLLEKLKVLDVTRNPLERVDRALAERCDIDLKADCLCVLASWDEVRRDNCSQQPPLQCLHTDTGTWHNVSAFLEAGCPPGLARTTMGVLAASAGLFLGLAVAGSVLAWRLRRHRASSSQGLSKTWAAQDGPWPGSGRQPRYSSRGLSPEPSGAALRRPSTPDYENMFVGQPPAGHQWADHGAQPSEDGDFYMNYKGPDHASQPIYGNLQSLDWAPEEYVMAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LJ49</accession>
    <name>A0A8C4LJ49_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">T-complex protein 1 subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">CCT-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CCT2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">Component of the chaperonin-containing T-complex (TRiC), a hexadecamer composed of two identical back-to-back stacked rings enclosing a protein folding chamber. Each ring is made up of eight different subunits: TCP1/CCT1, CCT2, CCT3, CCT4, CCT5, CCT6A/CCT6, CCT7, CCT8. Interacts with PACRG. Interacts with FLCN. Interacts with DLEC1. Interacts with SVEP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014714067.1">
        <property type="nucleotide sequence ID" value="XM_014858581.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LJ49"/>
    <dbReference type="Ensembl" id="ENSEAST00005012477.2">
        <property type="protein sequence ID" value="ENSEASP00005011475.2"/>
        <property type="gene ID" value="ENSEASG00005008081.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106842200"/>
    <dbReference type="KEGG" id="eai:106842200"/>
    <dbReference type="CTD" id="10576"/>
    <dbReference type="GeneTree" id="ENSGT00550000074930"/>
    <dbReference type="OrthoDB" id="10259763at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044297">
        <property type="term" value="C:cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002199">
        <property type="term" value="C:zona pellucida receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051131">
        <property type="term" value="P:chaperone-mediated protein complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904874">
        <property type="term" value="P:positive regulation of telomerase RNA localization to Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032212">
        <property type="term" value="P:positive regulation of telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090666">
        <property type="term" value="P:scaRNA localization to Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03336">
        <property type="entry name" value="TCP1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000046">
        <property type="entry name" value="Chaperonin containing TCP1 subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000002">
        <property type="entry name" value="T-complex protein 1 subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000045">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012716">
        <property type="entry name" value="Chap_CCT_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02341">
        <property type="entry name" value="chap_CCT_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 17" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00093360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011475.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="535" mass="57515" checksum="6B8B9FF4391A46E0" modified="2023-09-13" version="2">MASLSLAPVNIFKAGADEERAETARLSSFIGAIAIGDLVKSTLGPKGMDKILLSSGRDASLMVTNDGATILKNIGVDNPAAKVLVDMSRVQDDEVGDGTTSVTVLAAELLREAESLIAKKIHPQTIIAGWREATKAARQALLNSAVDHGSDEVKFRQDLMNIAGTTLSSKLLTHHRDHFTQLAVQAVLRLKGSGNLEAIHIIKKLGGSLADSYLDEGFLLDKKIGVNQPKRIENAKILIANTGMDTDKIKIFGSRVRVDSTAKVAEIEHAEKEKMKEKVERILKHGINCFINRQLIYNYPEQLFGGAGVMAIEHADFAGVERLALVTGGEIASTFDHPELVKLGSCKLIEEVMIGEDKLIHFSGVALGEACTIVLRGATQQILDEAERSLHDALCVLAQTVKDPRTVYGGGCSEMLMAHAVTELASRTPGKEAVAMESYAKALRMLPTIIADNAGYDSADLVAQLRAAHSEGNMTAGLDMKEGTIGDMAELGITESFQVKRQVLLSAAEAAEVILRVDNIIKAAPRKRVPDHHPC</sequence>
</entry>
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        <recommendedName>
            <fullName evidence="1">Sorting nexin/Vps5-like C-terminal domain-containing protein</fullName>
        </recommendedName>
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    <gene>
        <name evidence="2" type="primary">LOC123283099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LLX7"/>
    <dbReference type="Ensembl" id="ENSEAST00005015189.2">
        <property type="protein sequence ID" value="ENSEASP00005013980.2"/>
        <property type="gene ID" value="ENSEASG00005009746.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154940"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034452">
        <property type="term" value="F:dynactin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000166">
        <property type="entry name" value="Sorting NeXin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015404">
        <property type="entry name" value="Vps5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45850">
        <property type="entry name" value="SORTING NEXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45850:SF4">
        <property type="entry name" value="SORTING NEXIN-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09325">
        <property type="entry name" value="Vps5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sorting nexin/Vps5-like C-terminal" evidence="1">
        <location>
            <begin position="64"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005013980.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="144" mass="16630" checksum="48F104267C724688" modified="2023-09-13" version="2">MQVFFSKFQNCLIKTRKIEARVSADEDLKLSDLLKYYLRESQAAKDLLYRRSRSLVDYENANKALDKARAKNKDVLQAETSQQLCCQKFEKISESAKQELIDFKTRRVAAFRKNLVELAELELKHAKGNLQLLQNCLAVLNGDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LMD4</accession>
    <name>A0A8C4LMD4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">cAMP-dependent protein kinase inhibitor beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">PKIB</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Extremely potent competitive inhibitor of cAMP-dependent protein kinase activity, this protein interacts with the catalytic subunit of the enzyme after the cAMP-induced dissociation of its regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PKI family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LMD4"/>
    <dbReference type="Ensembl" id="ENSEAST00005014515.2">
        <property type="protein sequence ID" value="ENSEASP00005013365.1"/>
        <property type="gene ID" value="ENSEASG00005009319.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000064264"/>
    <dbReference type="OMA" id="KSHRCSC"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004171">
        <property type="entry name" value="cAMP_dep_PKI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15416">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE INHIBITOR/PKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02827">
        <property type="entry name" value="PKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00049">
        <property type="entry name" value="WILMSTUMOUR"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0649">Protein kinase inhibitor</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="201"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005013365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="234" mass="24135" checksum="53F08AF90997B44D" modified="2025-10-08" version="2">MSVNTHTGTRVLSPAPAGKSHRCSCPGSSAAAWAAPKAGHGPGATLCARGGSPRPLPPPPESPPPPGCAPAQPLRRGRIPADGRGGSELEPAALPRPPSGWASPSARGPGPGAAGGSARGWSHGILKSHSIKRNLGEAGNHQVIEATRGRRHRDVAMRTDSPEMTDVEPMVNNFASSARTGRRNAVPDIQGSAATGGTSELPHKLEALSMKEDVKKKGEETTQDQLKKPNNEAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LN85</accession>
    <name>A0A8C4LN85_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ankyrin repeat domain-containing protein 13C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ANKRD13C</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Acts as a molecular chaperone for G protein-coupled receptors, regulating their biogenesis and exit from the ER.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014722745.1">
        <property type="nucleotide sequence ID" value="XM_014867259.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LN85"/>
    <dbReference type="Ensembl" id="ENSEAST00005012112.2">
        <property type="protein sequence ID" value="ENSEASP00005011137.1"/>
        <property type="gene ID" value="ENSEASG00005007844.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106847781"/>
    <dbReference type="KEGG" id="eai:106847781"/>
    <dbReference type="CTD" id="81573"/>
    <dbReference type="GeneTree" id="ENSGT00950000182928"/>
    <dbReference type="OMA" id="YPMHQSV"/>
    <dbReference type="OrthoDB" id="1585644at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 16"/>
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    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000073">
        <property type="entry name" value="Ankyrin repeat domain-containing protein 13C"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR021832">
        <property type="entry name" value="ANKRD13"/>
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    <dbReference type="InterPro" id="IPR055285">
        <property type="entry name" value="ANKRD13_C"/>
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    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
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    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
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        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12447:SF25">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 13C"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF11904">
        <property type="entry name" value="ANKRD13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
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    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
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    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
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    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
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            <begin position="148"/>
            <end position="180"/>
        </location>
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            <begin position="264"/>
            <end position="521"/>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8C4LQG1_EQUAS</name>
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        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
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        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="Ensembl" id="ENSEAST00005016023.2">
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        <property type="gene ID" value="ENSEASG00005010258.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 18"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032183">
        <property type="term" value="F:SUMO binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02665">
        <property type="entry name" value="Peptidase_C19I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20486">
        <property type="entry name" value="USP25_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000004">
        <property type="entry name" value="Putative ubiquitin carboxyl-terminal hydrolase 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF666">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="11">
        <location>
            <begin position="70"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="370"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="624"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="664"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="587"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="376"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="664"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="675"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014733.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1025" mass="118893" checksum="1AB4467AFDD6D4D2" modified="2023-09-13" version="2">LKSKKNVIEQSSITLAINTLFFFSSFSPHGLRVLEASIAENKACLKRTPTEVWRDSRNPYDRKRQDKAPVGLKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSHAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQQDVSEFTHKLLDWLEDAFQMKAEEETDEEKPKNPMVELFYGRFLAVGVLEGKKFENTEMFGQYPLQVNGFKDLHECLEAAMIEGEIESLHSENSGKSGQEHWFTELPPVLTFELSRFEFNQALGRPEKIHNKLEFPQVLYLDRYMLRNREITRIKREEIKRLKDYLTVLQQRLERYLSYGSGPKRFPLVDVLQYALEFASSKPVCTSPIDDIDASSPPSGSVSSQTLPSTTEQQGTPSSELPSTSPASVAAISSRSVIHKPFTQSRIPPDLPMHPAPRHITEEELSVLESCLHRWRTEIENDTRDLQESISRIHRTIELMYSDKSMIQVPYRLHAVLVHEGQANAGHYWAYIFDHHENRWMKYNDIAVTKSSWEELVRDSFGGYRNASAYCLMYINDKAQFLIQEEFNKETGQALVGIETLPPDLRDFVEEDNQRFEKELEEWDTQLAQKALQEKLLASQKLRESESSITAAQAGEPEYLEQPSRSDFSKHLKEETVRLITKASHEHEDKSPETVLQSKPENTTSQPPSTQQVVEVAIPHVGKFMIEAKEGGYDDEIMMTPNMQGIIMAIGKSRNVYDRCGPEAGFFKAIKLEYSRLVKLSQEDTPPETDYRLHHVVVYFIQNEAPKKIIEKTLLEQFGDRNLSFDERCHNIMKVAQAKLEMIKPEEVNLEEYEEWHQDYRKFRETTMYLIIGLEYFQGERYIDSLLFLIYAYQNNKELLSKGQYRGHDEELLSHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNELIVPVLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMESNLQEKLTDFLPKLLDCSTEIKGFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LSI7</accession>
    <name>A0A8C4LSI7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription factor RFX3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Regulatory factor X 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RFX3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014683185.1">
        <property type="nucleotide sequence ID" value="XM_014827699.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044612439.1">
        <property type="nucleotide sequence ID" value="XM_044756504.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070352099.1">
        <property type="nucleotide sequence ID" value="XM_070495998.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070352100.1">
        <property type="nucleotide sequence ID" value="XM_070495999.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070352101.1">
        <property type="nucleotide sequence ID" value="XM_070496000.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LSI7"/>
    <dbReference type="Ensembl" id="ENSEAST00005016569.2">
        <property type="protein sequence ID" value="ENSEASP00005015233.2"/>
        <property type="gene ID" value="ENSEASG00005010402.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822479"/>
    <dbReference type="CTD" id="5991"/>
    <dbReference type="GeneTree" id="ENSGT01050000244879"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000017">
        <property type="entry name" value="transcription factor RFX3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003150">
        <property type="entry name" value="DNA-bd_RFX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039779">
        <property type="entry name" value="RFX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057321">
        <property type="entry name" value="RFX1-4/6/8-like_BCD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007668">
        <property type="entry name" value="RFX1_trans_act"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619">
        <property type="entry name" value="RFX TRANSCRIPTION FACTOR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12619:SF20">
        <property type="entry name" value="TRANSCRIPTION FACTOR RFX3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25340">
        <property type="entry name" value="BCD_RFX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04589">
        <property type="entry name" value="RFX1_trans_act"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02257">
        <property type="entry name" value="RFX_DNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51526">
        <property type="entry name" value="RFX_DBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RFX-type winged-helix" evidence="12">
        <location>
            <begin position="183"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="664"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="674"/>
            <end position="688"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005015233.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="749" mass="83556" checksum="21D497F37A2502B6" modified="2023-09-13" version="2">MQTSETGSDTGSTVTLQTSVASQAAVPTQVVQQVPVQQQVQQVQTVQQVQHVYPAQVQYVEGSDTVYTNGAIRTTTYPYTETQMYSQNTGGNYFDTQGSSAQVTTVVSSHSMVGTGGIQMGVTGGQLISSSGGTYLIGNSMENSGHSVTHTTRASPATIEMAIETLQKSDGLSTHRSSLLNSHLQWLLDNYETAEGVSLPRSTLYNHYLRHCQEHKLDPVNAASFGKLIRSIFMGLRTRRLGTRGNSKYHYYGIRVKPDSPLNRLQEDMQYMAMRQQPMQQKQRYKPMQKVDGVADGFTGSGQQTGTSVEQTVIAQSQHHQQFLDASRALPEFGEVEISSLPDGTTFEDIKSLQSLYREHCEAILDVVVNLQFSLIEKLWQTFWRYSPSTPTDGTTITESSNLSEIESRLPKAKLITLCKHESILKWMCNCDHGMYQALVEILIPDVLRPIPSALTQAIRNFAKSLEGWLSNAMNNIPQRMIQTKVAAVSAFAQTLRRYTSLNHLAQAARAVLQNTSQINQMLNDLNRVDFANVQEQASWVCQCDDNMVQRLETDFKMTLQQQSTLEQWAAWLDNVMMQALKPYEGRPSFPKAARQFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMFYLVEHRVAQATGETPIAVMGEFGDLNAVSPGNLDKDEGSEVESEMDEELDDSSEPQAKREKTELTPAFPVGCMQPVLESSVQPSLLNPIHSEHIVTSTQTIRQCSATGNTYTAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LT00</accession>
    <name>A0A8C4LT00_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ryanodine receptor 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">Skeletal muscle calcium release channel</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Skeletal muscle ryanodine receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Skeletal muscle-type ryanodine receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Type 1 ryanodine receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="33" type="primary">RYR1</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="27">Cytosolic calcium-activated calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytosol and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules. Repeated very high-level exercise increases the open probability of the channel and leads to Ca(2+) leaking into the cytoplasm. Can also mediate the release of Ca(2+) from intracellular stores in neurons, and may thereby promote prolonged Ca(2+) signaling in the brain. Required for normal embryonic development of muscle fibers and skeletal muscle. Required for normal heart morphogenesis, skin development and ossification during embryogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="28">Homotetramer. Can also form heterotetramers with RYR2. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1). Repeated very high-level exercise decreases interaction with PDE4D and protein phosphatase 1 (PP1). Interacts with CALM; CALM with bound calcium inhibits the RYR1 channel activity. Interacts with S100A1. Interacts with FKBP1A; this stabilizes the closed conformation of the channel. Interacts with CACNA1S; interaction with CACNA1S is important for activation of the RYR1 channel. Interacts with CACNB1. Interacts with TRDN and ASPH; these interactions stimulate RYR1 channel activity. Interacts with SELENON. Interacts with scorpion calcins (AC P0DPT1; AC P0DM30; AC A0A1L4BJ42; AC P59868; AC P60254; AC B8QG00; AC L0GBR1; AC P60252; AC P60253).</text>
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        <subcellularLocation>
            <location evidence="1">Sarcoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="153" mass="15813" checksum="DDE2070108680EF9" modified="2023-09-13" version="2">RVGPEEARRAALTGAGREGKPGGKLSAESRGAGARAAVLRTHSPPLVGRSVASEPGSSRPSPAQPLGPLASPARAAPRAPRPAPAMSELNTKTSPATNQAPGPEEKGKAGNAKKAEEEEEIDIDLTAPETEKAALAIQGKFRRFQKRKKDPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LZC2</accession>
    <name>A0A8C4LZC2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zinc finger protein 768</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ZNF768</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Binds to mammalian-wide interspersed repeat (MIRs) sequences in euchromatin and promoter regions of genes at the consensus sequence 5'-GCTGTGTG-[N20]-CCTCTCTG-3', consisting of two anchor regions connected by a linker region; the linker region probably does not contribute to the binding specificity. Required for cell homeostasis. May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts (via zinc-finger domains) with TP53 (via N-terminus); interaction might be facilitated by TP53 oligomerization state. Interacts with ELP3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014697880.2">
        <property type="nucleotide sequence ID" value="XM_014842394.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LZC2"/>
    <dbReference type="Ensembl" id="ENSEAST00005020446.2">
        <property type="protein sequence ID" value="ENSEASP00005018842.2"/>
        <property type="gene ID" value="ENSEASG00005027752.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106831913"/>
    <dbReference type="KEGG" id="eai:106831913"/>
    <dbReference type="CTD" id="79724"/>
    <dbReference type="GeneTree" id="ENSGT00940000162267"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000322">
        <property type="entry name" value="GDNF-inducible zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000953">
        <property type="entry name" value="Zinc finger protein 691"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000336">
        <property type="entry name" value="zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000628">
        <property type="entry name" value="zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000775">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000972">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001051">
        <property type="entry name" value="zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001784">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000684">
        <property type="entry name" value="RNA_pol_II_repeat_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF120">
        <property type="entry name" value="KRUPPEL-LIKE FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05001">
        <property type="entry name" value="RNA_pol_Rpb1_R"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="268"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="296"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="324"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="352"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="380"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="408"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="436"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="464"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="492"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="520"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="24"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="34"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="162"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00054040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00063122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00069136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005018842.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="547" mass="60744" checksum="E622278215191C35" modified="2023-09-13" version="2">MEREASPWGLEPQDVQSPDEMGSPQGSLQGNMSENEEEGISQQEDTGDYEVEEIPFGLEPQSPGFEPQNPEFESQSPRFEPESPGFESHSPGFVPPSPEFAPRSSESDSQSPEFEPQSPRYEPQSPGYEPKSPGYESQSSRYESKSPGYEPQNPVFEPQNPEFKTQSPEFEAQSSRFQEGAEMILNPEEKNPLSIPLGVHPLDSFTQGFGEQPTGGLPLGPPFEMPTGALLASPDFAMLQNPLGLTGTLRGPGRRGGRARGGQGPRPNICGICGKSFGRGSTLIQHQRIHTGEKPYKCEVCSKAFSQSSDLIKHQRTHTGERPYKCPRCGKAFADSSYLLRHQRTHSGQKPYKCPHCGKAFGDSSYLLRHQRTHSHERPYSCPECGKCYSQNSSLRSHQRVHTGQRPFSCGICGKSFSQRSALIPHARSHAREKPFKCPECGKRFGQSSVLAIHARTHLPGRTYSCPDCGKTFNRSSTLIQHQRSHTGERPYRCAVCGKGFCRSSTLLQHHRVHSGERPYKCDDCGKAFSQSSDLIRHQRTHAAGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M0H5</accession>
    <name>A0A8C4M0H5_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M0H5"/>
    <dbReference type="Ensembl" id="ENSEAST00005019567.2">
        <property type="protein sequence ID" value="ENSEASP00005018025.2"/>
        <property type="gene ID" value="ENSEASG00005012443.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000185040"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005018025.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="75" mass="8861" checksum="EA2D744BD8987F10" modified="2023-09-13" version="2">MHLEDTARFCPKEERESKQTSFRDQNLRQDQKGAFKTPSRSTLKREMGMQLGPHTNSLILESDQNHQKERCTVLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M1Q4</accession>
    <name>A0A8C4M1Q4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aspartate beta-hydroxylase domain containing 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ASPHD1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aspartyl/asparaginyl beta-hydroxylase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014719413.1">
        <property type="nucleotide sequence ID" value="XM_014863927.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M1Q4"/>
    <dbReference type="Ensembl" id="ENSEAST00005022188.2">
        <property type="protein sequence ID" value="ENSEASP00005020439.2"/>
        <property type="gene ID" value="ENSEASG00005014060.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106845618"/>
    <dbReference type="KEGG" id="eai:106845618"/>
    <dbReference type="CTD" id="253982"/>
    <dbReference type="GeneTree" id="ENSGT00940000161676"/>
    <dbReference type="OrthoDB" id="438431at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007803">
        <property type="entry name" value="Asp/Arg/Pro-Hydrxlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051821">
        <property type="entry name" value="Asp/Asn_beta-hydroxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46332:SF1">
        <property type="entry name" value="ASPARTATE BETA-HYDROXYLASE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46332">
        <property type="entry name" value="ASPARTATE BETA-HYDROXYLASE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05118">
        <property type="entry name" value="Asp_Arg_Hydrox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartyl/asparaginy/proline hydroxylase" evidence="6">
        <location>
            <begin position="228"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="86"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF05118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020439.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="367" mass="38854" checksum="BE0ECFF56268EC80" modified="2023-09-13" version="2">MWKGNSPGGNQGAAMEGTRGEQGGQGNWGLEDDPGLLARASLPIMPAWPLPLASSALTLLLGALTSLFLWYCYRLGSQDMQALGDERQARSVSGGPGGCSEAGRPSPGSAGEPGEGPRTEGLVSRRLRAYARRYSWAGMGRVRRAAQGGPGPGGGPGVLGIQRPGLLFLPDLPSAPFMPRDAQRHDVELLESSFPAILRDFGAVSWDFSGTTPLPRGWSPPLAPGCYQLLLYQAGQCQPSNCRLCPGAYRALRGLRSFISANTFGNAGFSVLLPGARLEGRCGPTNVRVRCHLGLKIPPGCELVVGGEPQCWAEGHCLLVDDSFLHTVAHNGSPEDGPRVVFIVDLWHPNVAGAERQALDFVFAPDP</sequence>
</entry>
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    <accession>A0A8C4M1U2</accession>
    <name>A0A8C4M1U2_EQUAS</name>
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        <recommendedName>
            <fullName evidence="16">Tyrosine-protein kinase</fullName>
            <ecNumber evidence="16">2.7.10.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">SYK</name>
    </gene>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15 26 27">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.10.2" evidence="16"/>
    <dbReference type="RefSeq" id="XP_014710152.1">
        <property type="nucleotide sequence ID" value="XM_014854666.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070351554.1">
        <property type="nucleotide sequence ID" value="XM_070495453.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070351555.1">
        <property type="nucleotide sequence ID" value="XM_070495454.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M1U2"/>
    <dbReference type="Ensembl" id="ENSEAST00005022067.2">
        <property type="protein sequence ID" value="ENSEASP00005020325.1"/>
        <property type="gene ID" value="ENSEASG00005013986.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106839739"/>
    <dbReference type="KEGG" id="eai:106839739"/>
    <dbReference type="CTD" id="6850"/>
    <dbReference type="GeneTree" id="ENSGT00940000159053"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019815">
        <property type="term" value="C:B cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032009">
        <property type="term" value="C:early phagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016170">
        <property type="term" value="F:interleukin-15 receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004715">
        <property type="term" value="F:non-membrane spanning protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
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        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="22">
        <location>
            <begin position="167"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="371"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="299"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="307"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="324"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="494"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="377"/>
            <end position="385"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18 20">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000604-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR000604-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="635" mass="71837" checksum="3FBDE2537DC361C7" modified="2022-01-19" version="1">MAGGTADGANNLPFFFGNITREEAEDYLVQGGMSDGLYLLRQSRNYLGGFALSVAHGRKAHHYTIERELNGTYAIAGGRTHSSPAELCHYHSQESDGLVCLLTKPFNRPPGVQPKTGPFEDLKENLIREYVKQTWNLQGEALEQAIISQKPQLEKLIATTAHEKMPWFHGKISRDESEQVVLIGSKINGKFLIRARDNNGSYALCLLHEGKVLHYRIDKDKTGKLSIPDGKKFDTLWQLVEHYSYKADGLLRVLTVPCQKIGGQTGNINFGGRPQLPSSHPATWSAGGIISRIKSYSFPKPGHRKSSSSQGNHPESSATSNPYELDRGTSATERDAQREAMPMDTEVYESPYADPEEIRPKEVYLDRNLLTLEDNELGSGNFGTVKKGYYQMKKVVKTVAVKILKNEANDPALKDELLAEANVMQQLDNPYIVRMIGICEAESWMLVMEMAELGPLNKYLQQNRHVKDKNIIELVHQVSMGMKYLEESNFVHRDLAARNVLLVTQHYAKISDFGLSKALRADESYYKAQTHGKWPVKWYAPECINYYKFSSKSDVWSFGVLMWEAFSYGQKPYRGMKGSEVSAMLEKGERMGCPAGCPREMYELMKLCWTYEVENRPGFVAVELRLRNYYYDVVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M3U5</accession>
    <name>A0A8C4M3U5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Glutathione S-transferase LANCL1</fullName>
            <ecNumber evidence="4">2.5.1.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">40 kDa erythrocyte membrane protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">LanC-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LANCL1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Functions as a glutathione transferase. Catalyzes conjugation of the glutathione (GSH) to artificial substrates 1-chloro-2,4-dinitrobenzene (CDNB) and p-nitrophenyl acetate. Mitigates neuronal oxidative stress during normal postnatal development and in response to oxidative stresses probably through GSH antioxidant defense mechanism. May play a role in EPS8 signaling. Binds glutathione.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>1-chloro-2,4-dinitrobenzene + glutathione = 2,4-dinitrophenyl-S-glutathione + chloride + H(+)</text>
            <dbReference type="Rhea" id="RHEA:51220"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:34718"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:133977"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 21">
            <text>RX + glutathione = an S-substituted glutathione + a halide anion + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16437"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16042"/>
            <dbReference type="ChEBI" id="CHEBI:17792"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="EC" id="2.5.1.18"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with the C-terminal of STOM. Interacts with the EPS8 SH3 domain. Interaction with EPS8 is inhibited by glutathione binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the LanC-like protein family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.18" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4M3U5"/>
    <dbReference type="Ensembl" id="ENSEAST00005021176.2">
        <property type="protein sequence ID" value="ENSEASP00005019519.2"/>
        <property type="gene ID" value="ENSEASG00005013420.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063186"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043295">
        <property type="term" value="F:glutathione binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004364">
        <property type="term" value="F:glutathione transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050750">
        <property type="term" value="F:low-density lipoprotein particle receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990748">
        <property type="term" value="P:cellular detoxification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031179">
        <property type="term" value="P:peptide modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04794">
        <property type="entry name" value="euk_LANCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000019">
        <property type="entry name" value="LanC-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007822">
        <property type="entry name" value="LANC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020464">
        <property type="entry name" value="LanC-like_prot_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12736:SF5">
        <property type="entry name" value="GLUTATHIONE S-TRANSFERASE LANCL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12736">
        <property type="entry name" value="LANC-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05147">
        <property type="entry name" value="LANC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01951">
        <property type="entry name" value="LANCEUKARYTE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01950">
        <property type="entry name" value="LANCSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM01260">
        <property type="entry name" value="LANC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158745">
        <property type="entry name" value="LanC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 18" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="18">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="323"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00035808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00039457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00063121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00079143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR607822-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005019519.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="412" mass="46742" checksum="4DA193C3DE8AA274" modified="2023-09-13" version="2">MAERALPNPYADYNRSLAEGYCDAAGALTAEFSQRLNNKIRELLQRMERGLKSADPRDGTAYTGWTGIAVLYLHLYDVFGDPGYLQMAHTYVKQGLNCLSKRSITFLCGDAGPLAVAAVVYHKMNNEKQAEDCITRLIHLNKLDSHAPNEMLYGRMGYIYALLFVNKHFGVEKIPQSHIQQICETVLTSGENLARKRNFTAKTPLMYEWYQEYYVGAAHGLAGIYYFLMQPSLQVSHAKLHSLVKPSVDYVCQLKFPSGNYPPCVDDTRDLLVHWCHGAPGVIYMLIQAYKVFREERYLTDARQSADLIWQYGLLKKGYGLCHGAAGNAYAFLALFNLTQDRKYLYRACKFAEWCLDYGEHGCRTPDTPFSLFEGMAGTIYFLADLLVPEKARFPAFELPKVRAPQFCIVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M3U7</accession>
    <name>A0A8C4M3U7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Matrix remodeling-associated protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">MXRA8</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044625380.1">
        <property type="nucleotide sequence ID" value="XM_044769445.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M3U7"/>
    <dbReference type="Ensembl" id="ENSEAST00005023017.2">
        <property type="protein sequence ID" value="ENSEASP00005021199.1"/>
        <property type="gene ID" value="ENSEASG00005014522.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106845232"/>
    <dbReference type="CTD" id="54587"/>
    <dbReference type="GeneTree" id="ENSGT00390000001509"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
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    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000806">
        <property type="entry name" value="Matrix remodeling associated 8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042472">
        <property type="entry name" value="MXRA8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44793">
        <property type="entry name" value="MATRIX REMODELING-ASSOCIATED PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44793:SF1">
        <property type="entry name" value="MATRIX REMODELING-ASSOCIATED PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 15" id="KW-0732">Signal</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034513691" description="Matrix remodeling-associated protein 8" evidence="15">
        <location>
            <begin position="24"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="29"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="16">
        <location>
            <begin position="161"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="295"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="444" mass="49777" checksum="4CC87EDAF5C4D0BB" modified="2022-01-19" version="1">MELRSRVLFWKLVLLQSSAVLLSSGPSGPGMAGSSVVSESVVSWAAGAQATLRCQSPRMVWTQDRLHDRQRVVHWDLSGGPAGGPTRRLVDMYSAGEQRVYETRDRGRLLLPPSAFHDGNFSLLIRAVEETDEGLYTCNLHHHYCHLYESLAVRLEVTDNPRAAGAHWDGEKEVLVAERGAPALLTCVNRAHVWTDRHLEEAQQVVHWDRQPPGVPHDRADRLLDLYASGERRAYGPPFLRDRVAVAVDAFARGDFSLRIDPLEPADEGTYSCHLHHHYCGLHERRVFHLRVTEPAAQPPPRDSPGNGSSHSSAPRPDPTLARGRSVINVIVPEGRAHFFQQLGYVLATLLLFILLLITVILATRQRRRGGYEYSGKKSGRSKGKDMNMAEFAVATGDQPLYRTEDIQLDYKNNILKERAGLAHSPLPAKYVDLDNEFRKEYCK</sequence>
</entry>
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    <accession>A0A8C4M8C9</accession>
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        <submittedName>
            <fullName evidence="6">Phosphatidylinositol specific phospholipase C X domain containing 3</fullName>
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    <gene>
        <name evidence="6" type="primary">PLCXD3</name>
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        <name type="synonym">Equus africanus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_014867421.3"/>
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    <dbReference type="Ensembl" id="ENSEAST00005021910.2">
        <property type="protein sequence ID" value="ENSEASP00005020182.1"/>
        <property type="gene ID" value="ENSEASG00005013901.2"/>
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    <dbReference type="GeneTree" id="ENSGT00940000158747"/>
    <dbReference type="OMA" id="ALPAMMH"/>
    <dbReference type="OrthoDB" id="1046782at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0016042">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08616">
        <property type="entry name" value="PI-PLCXD1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000021">
        <property type="entry name" value="PI-PLC X domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042158">
        <property type="entry name" value="PLCXD1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF33">
        <property type="entry name" value="PI-PLC X DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
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        <property type="entry name" value="PLC-like phosphodiesterases"/>
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        <property type="entry name" value="PIPLC_X_DOMAIN"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="3" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="Phosphatidylinositol-specific phospholipase C X" evidence="5">
        <location>
            <begin position="22"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="321" mass="36329" checksum="33BF873E38CC6265" modified="2022-01-19" version="1">MASSQGKNELKFADWMATLPESIHSIPLTNLAIPGSHDSFSFYIDEASPVGPEQPETVQNFVSVFGTVAKKLMRKWLATQTMNFTGQLGAGIRYFDLRISTKPRDPDNELYFAHGLFSAKVNEGLEEINAFLTDHHKEVVFLDFNHFYGMQKYHHEKLVQMLKDIYGNKMCPAIFAQEVSLKYLWEKDYQVLVFYHSPVALEVPFLWPGQMMPAPWANTTDPEKLIQFLQASITERRKKGSFFISQVVLTPKASTVVKGVASGLRETITERALPAMMQWVRTQKPGESGINIVTADFVELGDFISTVIKLNYVFDEGEANT</sequence>
</entry>
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    <accession>A0A8C4MCR2</accession>
    <name>A0A8C4MCR2_EQUAS</name>
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        <recommendedName>
            <fullName evidence="8">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106836515</name>
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    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MCR2"/>
    <dbReference type="SMR" id="A0A8C4MCR2"/>
    <dbReference type="Ensembl" id="ENSEAST00005027052.2">
        <property type="protein sequence ID" value="ENSEASP00005024927.2"/>
        <property type="gene ID" value="ENSEASG00005016951.2"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 16"/>
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        <property type="term" value="C:myosin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000010">
        <property type="entry name" value="Myosin regulatory light chain 2, atrial isoform"/>
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    </dbReference>
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        <property type="entry name" value="Putative myosin regulatory light chain sqh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
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    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050403">
        <property type="entry name" value="Myosin_RLC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23049">
        <property type="entry name" value="MYOSIN REGULATORY LIGHT CHAIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0505">Motor protein</keyword>
    <keyword evidence="6" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="4" id="KW-0518">Myosin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="29"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="98"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005024927.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="172" mass="19794" checksum="3B85975E49FBA8FC" modified="2023-09-13" version="2">MSSKRAKAKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASMGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNSFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVDFTCILKHGAKDKDD</sequence>
</entry>
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        <name evidence="6" type="primary">AKR7A2</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the aldo/keto reductase family. Aldo/keto reductase 2 subfamily.</text>
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        <property type="nucleotide sequence ID" value="XM_014854744.3"/>
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    <dbReference type="Ensembl" id="ENSEAST00005023774.2">
        <property type="protein sequence ID" value="ENSEASP00005021913.2"/>
        <property type="gene ID" value="ENSEASG00005014962.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106839786"/>
    <dbReference type="KEGG" id="eai:106839786"/>
    <dbReference type="CTD" id="8574"/>
    <dbReference type="GeneTree" id="ENSGT00940000158496"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19075">
        <property type="entry name" value="AKR_AKR7A1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000017">
        <property type="entry name" value="Aflatoxin B1 aldehyde reductase member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050523">
        <property type="entry name" value="AKR_Detox_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364:SF4">
        <property type="entry name" value="NAD(P)-LINKED OXIDOREDUCTASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43364">
        <property type="entry name" value="NADH-SPECIFIC METHYLGLYOXAL REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="5">
        <location>
            <begin position="56"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005021913.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="374" mass="40890" checksum="167446A2A4359E6E" modified="2023-09-13" version="2">MLAAASRAVARAVGRAGVRCAPRPGQPEARTATAAMSRLLSTPRAASDAGVRPATVLGTMEMGRRMDAPASAAAVRAFLERGHTELDTAFMYSDGQSESILGGLGLGLGGGDCKVKIATKANPWEGKSLKPDCVRSQLETSLKRLQCPRVDLFYLHAPDHSTPVEETLRACHQLHQEGKFVELGLSNYASWEVAEICTLCKSNGWIVPTVYQGMYNATTRRVETELFPCLRHFGLRFYAYNPLAGGLLTGKYKYEDKDGKQPAGRFFGNSWAEVYRNRFWKEHHFEAIALVEKALQAADGTSAPSMTSAALRWMYHHSQLQGALGDAVILGMSSMQQLAQNLAAAEEGPLEPAVVQAFDEAWHLVAHECPNYFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MGE8</accession>
    <name>A0A8C4MGE8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAST kinase domain-containing protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Protein TBRG4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Transforming growth factor beta regulator 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TBRG4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Plays a role in processing of mitochondrial RNA precursors and in stabilization of a subset of mature mitochondrial RNA species, such as MT-CO1, MT-CO2, MT-CYB, MT-CO3, MT-ND3, MT-ND5 and MT-ATP8/6. May play a role in cell cycle progression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the FAST kinase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MGE8"/>
    <dbReference type="Ensembl" id="ENSEAST00005028521.2">
        <property type="protein sequence ID" value="ENSEASP00005026265.2"/>
        <property type="gene ID" value="ENSEASG00005017915.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234607"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035770">
        <property type="term" value="C:ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000963">
        <property type="term" value="P:mitochondrial RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044528">
        <property type="term" value="P:regulation of mitochondrial mRNA stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23739">
        <property type="entry name" value="TBRG4-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013579">
        <property type="entry name" value="FAST_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050870">
        <property type="entry name" value="FAST_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010622">
        <property type="entry name" value="FAST_Leu-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013584">
        <property type="entry name" value="RAP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228:SF59">
        <property type="entry name" value="FAST KINASE DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21228">
        <property type="entry name" value="FAST LEU-RICH DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06743">
        <property type="entry name" value="FAST_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08368">
        <property type="entry name" value="FAST_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08373">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00952">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51286">
        <property type="entry name" value="RAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="RAP" evidence="9">
        <location>
            <begin position="452"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00042265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00043220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026265.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="522" mass="59106" checksum="2B2A444BC51EB6E3" modified="2023-09-13" version="2">MAARLVKRCTCLLREAALLAPAVSPVGRLRLARVAHKTLTSSATSPSSHFPGSLSELVEREQVFTPYPERQEVDHLIEKAARPEELLDLLGGGHCLHQNHAALILIRLSRLLSEKPGDKASLVRDARFQQLLHLVNSQITTVWHGTLVKLLRSLYALALPSGSKELQSVEQEVRWRMRRLKYKHLAFLAESSTTYMQERESRELLAELLMHLERRWAEIEDGRTVVAMMMKVGHLSESLMNRLEDKHVLNRAQSTTVPHLCNMLLAFARLNFRPEREDQFFSLVREKLGSELAGLDPALQVDVVWALCVLQQVREAELRAVLRPEFHTQFLGGESPKDQSIFQKLLHINATAQLEHPEYTSPLLPASALIPRLSALDKKVTPLQKELQETLKGLLGSSDRGSFMVATQYGWVLDAEVLLDADSQFLPLRDFVAPHLTPPSGSQPLPPGAKRLAFLRWEFPNFNSRSKDLLGRFVLARRHVLAAGFLVVDVPYYEWLELKSEWQKGAYLKDKIRKAVAEELAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MIM7</accession>
    <name>A0A8C4MIM7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">cAMP-dependent protein kinase type I-alpha regulatory subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">PRKAR1A</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKACA and PRKACB. PRKAR1A also interacts with RFC2; the complex may be involved in cell survival. Interacts with AKAP4. Interacts with RARA; the interaction occurs in the presence of cAMP or FSH and regulates RARA transcriptional activity. Interacts with the phosphorylated form of PJA2. Interacts with CBFA2T3. Interacts with PRKX; regulates this cAMP-dependent protein kinase. Interacts with smAKAP; this interaction may target PRKAR1A to the plasma membrane. Interacts with AICDA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014703157.1">
        <property type="nucleotide sequence ID" value="XM_014847671.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070339600.1">
        <property type="nucleotide sequence ID" value="XM_070483499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MIM7"/>
    <dbReference type="SMR" id="A0A8C4MIM7"/>
    <dbReference type="Ensembl" id="ENSEAST00005026471.2">
        <property type="protein sequence ID" value="ENSEASP00005024400.2"/>
        <property type="gene ID" value="ENSEASG00005016108.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835360"/>
    <dbReference type="KEGG" id="eai:106835360"/>
    <dbReference type="CTD" id="5573"/>
    <dbReference type="GeneTree" id="ENSGT00940000155148"/>
    <dbReference type="OrthoDB" id="417078at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001772">
        <property type="term" value="C:immunological synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031594">
        <property type="term" value="C:neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031588">
        <property type="term" value="C:nucleotide-activated protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044853">
        <property type="term" value="C:plasma membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120212">
        <property type="term" value="C:sperm head-tail coupling apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Fang"/>
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                <person name="Zhao"/>
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                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="G."/>
                <person name="Liu"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        </source>
    </evidence>
    <sequence length="355" mass="37535" checksum="F1AF807D2C689A9C" modified="2022-01-19" version="1">MAGVACLGKASDADEWCDSGLGSLGPDAAAPGGPGLGAELGPGLSWAPLVFGYVTEDGDTALHLAVIHQHEPFLDFLLGFAAGTEYLDLQNDLGQTALHLAAILEEASTVEKLYAAGAGLRVAERGGHTALHLACRVGAHACARVLLQPRPRRPRGAPSTYLAQDPDHTPNTDHTPVALYPDPDLEKEEDESEEDWKLQLEAENYEGHTPLHVAVIHKDAEMVQLLREAGADLNKPEPTCGRSPLHLAVEAQAADVLELLLRAGADPTARMYGGRTPLGSATLRPNPILARLLRAHGAPEPEDEDEKPGPCSSSSSDSDSGDEGDEYDDIVVHRGRSQNPLPPAPASKPLPDDPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MPG9</accession>
    <name>A0A8C4MPG9_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Family with sequence similarity 81 member B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">FAM81B</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM81 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MPG9"/>
    <dbReference type="Ensembl" id="ENSEAST00005031106.2">
        <property type="protein sequence ID" value="ENSEASP00005028635.2"/>
        <property type="gene ID" value="ENSEASG00005019474.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004985"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029619">
        <property type="entry name" value="FAM81"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22420">
        <property type="entry name" value="PROTEIN FAM81A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22420:SF5">
        <property type="entry name" value="PROTEIN FAM81B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="296"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00046344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005028635.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="412" mass="47582" checksum="A4730D5AE01EC7F3" modified="2023-09-13" version="2">MSSDTDVNKSASPTAEEQPDEPDGPLPGSASDQEKKVRVSPAKMSTKNSTDLVEYVDKGHSFLPIIPHPARSQLEDRLNNQERTIAFLLEQAFRIKEDISACLQGTHGFRKEESLARKLLESHIQTITSIVKKLSQNIEILEDQIRVRDQVATGTNFAVQELNTKHLQGVGDLRGRVARCDSSIVKLSADIHFIRHEHRQIEKTIQELVSALETISKNLDVKVMQLLGRLETSSSEQISNFKMLQGDYRHEMNILEFKFNSLSNNLCEEVENNKKWTENQFIKYQQDLLGHINQCLKLLQEKLEKSENKMEEKLLRLSSKLENFINTQKQEAELNKVKHIENKLSKKMNHMEKHIWGELEKMQNEYQSGFQSIHDSLSSLQQIQKTKMDLEKYKVRKDLKKLQRKIGELQEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MSX8</accession>
    <name>A0A8C4MSX8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transmembrane protease serine</fullName>
            <ecNumber evidence="10">3.4.21.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TMPRSS11D</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the peptidase S1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4MSX8"/>
    <dbReference type="Ensembl" id="ENSEAST00005029224.2">
        <property type="protein sequence ID" value="ENSEASP00005026916.2"/>
        <property type="gene ID" value="ENSEASG00005018344.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161719"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.960">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017329">
        <property type="entry name" value="Pept_S1A_HAT/DESC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF17">
        <property type="entry name" value="SUPPRESSOR OF TUMORIGENICITY 14 PROTEIN HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037941">
        <property type="entry name" value="TMPRSS11ABCDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00200">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50024">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 10 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 10 12" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10 12" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="16"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="domain" description="SEA" evidence="14">
        <location>
            <begin position="46"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="15">
        <location>
            <begin position="187"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11">
        <location>
            <position position="272"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="11">
        <location>
            <position position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037941-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50024"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026916.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="418" mass="46237" checksum="D60B61F7A3302036" modified="2023-09-13" version="2">MYRPARAPSTSRFLNPYVVCLLVVAGVVILAVTIALLVYFLAFDQKSHFYRSSFQILNVQYSDQLNSPATQEYRTLSGRIESMITKTLKESNLRNQFIKAHVVKIRQSGSGVIADTVMKFKFTRPNSGALMKRRIEAVLHQVLNNSGNLEINPSTEITQITDQDTVNILTKECGARPDLITLSEERIIGGSRAEEGDWPWQVSLHLNNVHHCGGILISDMWILTAAHCFRSSSDPRQWTATFGISTAFPRQRRGVRTIKTHNNYRPATHENDIAVLELDRSITFTANIHSICLPAATQNIPPGSTAYVTGWGSRRYGGSTVTDLEQAQVYIISNDVCNAPANYNGAVLPGMLCAGVPQGGVDACQGDSGGPLVQEDSRRVWFLVGIVSWGYQCGLPDKPGVYTRVTAYRDWITEQTGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MTD4</accession>
    <name>A0A8C4MTD4_EQUAS</name>
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        <recommendedName>
            <fullName evidence="13">Pannexin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PANX2</name>
        <name evidence="13" type="synonym">PANX</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Structural component of the gap junctions and the hemichannels.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the pannexin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014719988.1">
        <property type="nucleotide sequence ID" value="XM_014864502.3"/>
    </dbReference>
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    <dbReference type="GlyCosmos" id="A0A8C4MTD4">
        <property type="glycosylation" value="1 site, No reported glycans"/>
    </dbReference>
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        <property type="gene ID" value="ENSEASG00005021345.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106846000"/>
    <dbReference type="KEGG" id="eai:106846000"/>
    <dbReference type="CTD" id="56666"/>
    <dbReference type="GeneTree" id="ENSGT00940000153972"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005921">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022829">
        <property type="term" value="F:wide pore channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007267">
        <property type="term" value="P:cell-cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006812">
        <property type="term" value="P:monoatomic cation transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034220">
        <property type="term" value="P:monoatomic ion transmembrane transport"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032732">
        <property type="term" value="P:positive regulation of interleukin-1 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002931">
        <property type="term" value="P:response to ischemia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000990">
        <property type="entry name" value="Innexin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039099">
        <property type="entry name" value="Pannexin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15759">
        <property type="entry name" value="PANNEXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PANNEXIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00876">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 13" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 13" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="53"/>
            <end position="70"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="123"/>
            <end position="145"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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            <end position="250"/>
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        <location>
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        <location>
            <begin position="395"/>
            <end position="426"/>
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        <location>
            <position position="86"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU010713"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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</entry>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
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                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
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                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
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                <person name="Liu"/>
                <person name="G."/>
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                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="J."/>
                <person name="Liu"/>
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                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    <dbReference type="PANTHER" id="PTHR21580:SF62">
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        <location>
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            <end position="165"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005031514.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="277" mass="29625" checksum="D07204CF4F8D1457" modified="2023-09-13" version="2">MGTLGCNPAPRLTTAPLGRRATECQIPETGLRKTCGAATVEKGSGPGLYVLPSTVGYANHDCTKVTGPAYSLFRRPSEACPQDASPGPIYFLDPNVTRFGRSCTPAYSMQGRGKYRALEVTPGPGAYSPEKMAPVRQRTPPAFTLGSRLRPQPPDTSAPAPNTYTLPSLWGSQIFTKPSSPSYSVVGRTPPARPPQDPAEIPGPGQYDSPDPNAYRQRRPAFTMLGRPRAPRPRDETPGPGTHSPEQVTVTKARAPAFTMGIRHSRRAATMAADTTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MWE1</accession>
    <name>A0A8C4MWE1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 19">Zinc finger CCCH domain-containing protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">ZC3H14</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">RNA-binding protein involved in the biogenesis of circular RNAs (circRNAs), which are produced by back-splicing circularization of pre-mRNAs. Acts by binding to both exon-intron boundary and 3'-UTR of pre-mRNAs to promote circRNA biogenesis through dimerization and the association with the spliceosome. Required for spermatogenesis via involvement in circRNA biogenesis. Regulates the pre-mRNA processing of ATP5MC1; preventing its degradation. Also binds the poly(A) tail of mRNAs; controlling poly(A) length in neuronal cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homodimer; facilitating circular RNAs (circRNAs) formation. Associates with the spliceosome. Interacts with HOOK2. Interacts with ZFC3H1 in a RNase-sensitive manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 19">Belongs to the ZC3H14 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MWE1"/>
    <dbReference type="Ensembl" id="ENSEAST00005032549.2">
        <property type="protein sequence ID" value="ENSEASP00005029951.2"/>
        <property type="gene ID" value="ENSEASG00005020371.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00440000038430"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904115">
        <property type="term" value="C:axon cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032839">
        <property type="term" value="C:dendrite cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003730">
        <property type="term" value="F:mRNA 3'-UTR binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036002">
        <property type="term" value="F:pre-mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048255">
        <property type="term" value="P:mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160091">
        <property type="term" value="P:spliceosome-depend formation of circular RNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1390.10:FF:000006">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.30:FF:000001">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.40:FF:000006">
        <property type="entry name" value="Zinc finger CCCH domain-containing protein 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.40:FF:000001">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 14 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1000.30:FF:000003">
        <property type="entry name" value="zinc finger CCCH domain-containing protein 14 isoform X6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1000.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1390.10">
        <property type="entry name" value="PWI domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040366">
        <property type="entry name" value="Nab2/ZC3H14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14738">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14738:SF29">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14608">
        <property type="entry name" value="zf-CCCH_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6 18 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15 19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 19" id="KW-0677">Repeat</keyword>
    <keyword evidence="13 19" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="12" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="10 18 19" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 18 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="21">
        <location>
            <begin position="590"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="18">
        <location>
            <begin position="590"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="77"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="310"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="77"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="132"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00062976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU369058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005029951.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="731" mass="82343" checksum="2EFE0908B24EB2DA" modified="2023-09-13" version="2">MEIGTEISRKIRSAIKGKLQELGAYVDEELPDYIMVMVANKKSQDQMTEDLSLFLGNNTIRFTVWLHGVLDKLRSVTTDPSSLKSSDTNIFDNNVPSNKSSFSRGDERRHEAAVPPLAVSSTRPEKRDSRVSAGSQEQKTTSVRQTYDDGAATRLMSTVKPLREPAPSEDVIDIKPEPDDLIDEDLNFVQENPLSQKKPTVTLTYGSSRPSIEIYRPPASRNADSGAHLNRLQFQQQQNSIHAVKQLDMQNSRVYETGRLCEPEVLNSLEETYSPFFRNNAEKMSIEEENFRKRKLPVVSSVVKVKKFNHDGEEEEEDDDCGSRTGSISSSVSVPAKPERRPSLPPSKQANKNLILKAISEAQESVTKTTNYSTVSQKQTLPVAPRTRTSQEELLAEMVQGQSRTPRISPPIKEEEIKGDNIEKSQGTQQRQLLSRLQIDPVVAETLQMSQDYYDMESMVHADTRSFILKKPKLSEEIVVAPNQESGMKTADTLRVLSGHLMQTRDLVQPDKPASPKFIVTLDGVPSPPGYMSDQEEDMCFEGMKPVNQTAASNKGLRGLLHPQQLHLMSRQLDDPNAEMSELSVAQKPEKLLERCKYWPACKNGDECAYHHPVSPCKAFPNCKFAEKCLFVHPNCKYDAKCTKPDCPFTHMSRRIPVLPPKPAVTTPAPPSSSQLCRYFPACKKMECPFYHPKHCRFNTQCTRPDCTFYHPTITVPPRHALKWIRPQTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MXA4</accession>
    <name>A0A8C4MXA4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Endophilin-A2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Endophilin-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">SH3GL1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="X. and Zhong"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="12">Implicated in endocytosis. May recruit other proteins to membranes with high curvature.</text>
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        <subcellularLocation>
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            <location evidence="1">Podosome</location>
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        <subcellularLocation>
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                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a fatty acyl-CoA + sphing-4-enine = an N-acylsphing-4-enine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23768"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:52639"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57756"/>
            <dbReference type="ChEBI" id="CHEBI:77636"/>
            <dbReference type="EC" id="2.3.1.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:23769"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>docosanoyl-CoA + sphinganine = N-docosanoylsphinganine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36535"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57817"/>
            <dbReference type="ChEBI" id="CHEBI:65059"/>
            <dbReference type="ChEBI" id="CHEBI:67021"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:36536"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>eicosanoyl-CoA + sphinganine = N-eicosanoylsphinganine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36555"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57380"/>
            <dbReference type="ChEBI" id="CHEBI:57817"/>
            <dbReference type="ChEBI" id="CHEBI:67027"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:36556"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>hexacosanoyl-CoA + sphinganine = N-hexacosanoylsphinganine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33351"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:52962"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57817"/>
            <dbReference type="ChEBI" id="CHEBI:64868"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:33352"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>sphing-4-enine + octadecanoyl-CoA = N-octadecanoylsphing-4-enine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36691"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:57756"/>
            <dbReference type="ChEBI" id="CHEBI:72961"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:36692"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>sphinganine + octadecanoyl-CoA = N-(octadecanoyl)-sphinganine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36547"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:57817"/>
            <dbReference type="ChEBI" id="CHEBI:67033"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:36548"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>tetracosanoyl-CoA + sphinganine = N-tetracosanoylsphinganine + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33591"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:52961"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57817"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:33592"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; sphingolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Sphingolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19 21">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.1.24" evidence="18"/>
    <dbReference type="RefSeq" id="XP_014718358.2">
        <property type="nucleotide sequence ID" value="XM_014862872.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PHF7"/>
    <dbReference type="SMR" id="A0A8C4PHF7"/>
    <dbReference type="Ensembl" id="ENSEAST00005006355.2">
        <property type="protein sequence ID" value="ENSEASP00005005815.2"/>
        <property type="gene ID" value="ENSEASG00005004290.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106845048"/>
    <dbReference type="KEGG" id="eai:106845048"/>
    <dbReference type="CTD" id="91012"/>
    <dbReference type="GeneTree" id="ENSGT01030000234515"/>
    <dbReference type="OMA" id="FTESTWR"/>
    <dbReference type="UniPathway" id="UPA00222"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050291">
        <property type="term" value="F:sphingosine N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000020">
        <property type="entry name" value="Ceramide synthase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016439">
        <property type="entry name" value="Lag1/Lac1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006634">
        <property type="entry name" value="TLC-dom"/>
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    <dbReference type="PANTHER" id="PTHR12560:SF8">
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        <property type="entry name" value="LONGEVITY ASSURANCE FACTOR 1 LAG1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03798">
        <property type="entry name" value="TRAM_LAG1_CLN8"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50922">
        <property type="entry name" value="TLC"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="19 21" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 20 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="19 21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 20 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="208"/>
            <end position="228"/>
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    <feature type="transmembrane region" description="Helical" evidence="23">
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            <begin position="248"/>
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    <feature type="transmembrane region" description="Helical" evidence="23">
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            <begin position="274"/>
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    <feature type="transmembrane region" description="Helical" evidence="23">
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            <begin position="332"/>
            <end position="351"/>
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    <feature type="transmembrane region" description="Helical" evidence="23">
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            <begin position="371"/>
            <end position="396"/>
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            <begin position="152"/>
            <end position="196"/>
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    </feature>
    <feature type="domain" description="TLC" evidence="25">
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            <begin position="199"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="19">
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            <begin position="154"/>
            <end position="197"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="22">
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            <begin position="1"/>
            <end position="40"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="22">
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            <begin position="408"/>
            <end position="452"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="22">
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            <begin position="1"/>
            <end position="15"/>
        </location>
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            <dbReference type="SAM" id="Phobius"/>
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    <sequence length="452" mass="52095" checksum="126A283F6AF8288C" modified="2023-09-13" version="2">MGGEEAGPYGGGGLRCAGSKKGPEGGGRQSGPAPGPLPGGFRWLRRQLRLRRQWPQGGRKMATAVAGALGLLWGWLWSERFWLPQNVSWADLEGPADGYGYPRARHILSVFPLAAGIFFVRRLFERFIAKPCARHVGIQDSGPYQTQPNAILEKVFISITKYPDEKRLEGLSKQLDWDVRKIQCWFRHRRNQDKPPTLTKFCESMWRFTFYLCIFCYGIRFLWSSTWFWDTRQCWHSYPYQSLTSGLYYYYIMELAFYWSLMFSQFTDIKRKDFLIMFVHHLATIGLITFSYINNMVRVGTLVMCLHDASDFLLEAAKLANYAKYQRLCDTLFVIFGAVFVATRLGIYPFWVLNTTLFESWEMIGPYPSWWLFNGLLLILQVLHIIWSYLIMRIAFKALIRGKVSKDDRSDVESSSEEEDVTTSTKSPRGGNSSNGANRVNGHMGGSYWAEE</sequence>
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<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PIK9</accession>
    <name>A0A8C4PIK9_EQUAS</name>
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        <recommendedName>
            <fullName evidence="11">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">POU6F1</name>
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    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Transcription factor that binds preferentially to a variant of the octamer motif (5'-ATGATAAT-3').</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the POU transcription factor family. Class-6 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044612031.1">
        <property type="nucleotide sequence ID" value="XM_044756096.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PIK9"/>
    <dbReference type="Ensembl" id="ENSEAST00005009047.2">
        <property type="protein sequence ID" value="ENSEASP00005008300.1"/>
        <property type="gene ID" value="ENSEASG00005005986.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835225"/>
    <dbReference type="KEGG" id="eai:106835225"/>
    <dbReference type="CTD" id="5463"/>
    <dbReference type="GeneTree" id="ENSGT00940000160106"/>
    <dbReference type="OMA" id="SEMQPIQ"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000051">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000013">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF6">
        <property type="entry name" value="POU DOMAIN, CLASS 6, TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="14">
        <location>
            <begin position="449"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="542"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="544"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="39"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="375"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="611" mass="63191" checksum="3BA98A9C0E959AD3" modified="2022-01-19" version="1">MDPGAGPESSLTVNEQVIVMSGHETIRVLEVGVDAQIPAEEESKGLEGVASKGSQSRGPAEASEPAAEAGPDNPDSSAEATVKSLPGIAPSPAPAVATFSQAPSQPQSSQTLTPLAVQAASQVLTQENLATVLTGVMVPAGAVTQPLLIPISIAGQVAGQQGLAVWTIPTATVAALPGLTAASPTGAIFKPPLAGLQAAAVLNATLPAPVQAAAPVQASSPAQPRPPAQPQTLFQTQPLLQTTPAILPQPTAATATAPTPKPVDTPSQITVQPAGFAFSPGIISAASLGGQTQILGSLTTTPVIANAIPSMPGISSQILTNAQGQVIGTLPWVVNSASVAAPAPAQSLQVQAVTPQLLLNAQGQVIATLASSPLPPPVAVRKPSTPESPAKSEVQPIQPTPAVPPPAVVIASPAPAAKPSASAPIPITCSETPTVSQLVSKPHTPSLDEDGINLEEIREFAKNFKIRRLSLGLTQTQVGQALTATEGPAYSQSAICRFEKLDITPKSAQKLKPVLEKWLNEAELRNQEGQQNLMEFVGGEPSKKRKRRTSFTPQAIEALNAYFEKNPLPTGQEITEIAKELNYDREVVRVWFCNRRQTLKNTSKLNVFQIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PJJ4</accession>
    <name>A0A8C4PJJ4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MAGE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">LOC106835932</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_014704020.3">
        <property type="nucleotide sequence ID" value="XM_014848534.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PJJ4"/>
    <dbReference type="Ensembl" id="ENSEAST00005010615.2">
        <property type="protein sequence ID" value="ENSEASP00005009774.2"/>
        <property type="gene ID" value="ENSEASG00005006949.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835932"/>
    <dbReference type="KEGG" id="eai:106835932"/>
    <dbReference type="GeneTree" id="ENSGT00940000163033"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1200:FF:000007">
        <property type="entry name" value="Melanoma-associated antigen C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1210:FF:000001">
        <property type="entry name" value="melanoma-associated antigen D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1200">
        <property type="entry name" value="MAGE homology domain, winged helix WH1 motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1210">
        <property type="entry name" value="MAGE homology domain, winged helix WH2 motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037445">
        <property type="entry name" value="MAGE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021072">
        <property type="entry name" value="MAGE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041898">
        <property type="entry name" value="MAGE_WH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041899">
        <property type="entry name" value="MAGE_WH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002190">
        <property type="entry name" value="MHD_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11736:SF35">
        <property type="entry name" value="MELANOMA-ASSOCIATED ANTIGEN B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11736">
        <property type="entry name" value="MELANOMA-ASSOCIATED ANTIGEN MAGE ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01454">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12440">
        <property type="entry name" value="MAGE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01373">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01392">
        <property type="entry name" value="MAGE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50838">
        <property type="entry name" value="MAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MAGE" evidence="2">
        <location>
            <begin position="112"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009774.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="348" mass="39250" checksum="D4A2289AE60483D0" modified="2023-09-13" version="2">MPRGQKSKRRTREKRRHTQDDSQSDRNIQATAATEEEPPSSSSPVLEGNSQSLSAVESSSTSHGSWMASSTSINSPGIFGRISHEGDNNQGEERPHSSQVLLSTGNSCSDILNIKADLLAQYLLYKYKMKQPILKEDMLRLVHRNYEDQFAEILKRASELIELAFAVEVKEADSTSHSYDLVSKLKLPNNGRVRGGIGLPKTGLLMNILGMIFIKGNHVTEEEIWKFLNMMQVYAGRKHYIYGEPRKLITKDLVRLQYLEYQQVPNSDPPRYEFLWGPKAHAETSKMEVLEFMAKINDTVPTTFPLQYEEALQDEEERALARVAARPGTTGHCQACSRVASSSFSHPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PKP3</accession>
    <name>A0A8C4PKP3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Acyltransferase</fullName>
            <ecNumber evidence="11">2.3.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">DGAT2L6</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Endoplasmic reticulum membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the diacylglycerol acyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.-" evidence="11"/>
    <dbReference type="RefSeq" id="XP_014691089.1">
        <property type="nucleotide sequence ID" value="XM_014835603.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PKP3"/>
    <dbReference type="Ensembl" id="ENSEAST00005013929.2">
        <property type="protein sequence ID" value="ENSEASP00005012817.1"/>
        <property type="gene ID" value="ENSEASG00005008959.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106827655"/>
    <dbReference type="KEGG" id="eai:106827655"/>
    <dbReference type="CTD" id="347516"/>
    <dbReference type="GeneTree" id="ENSGT01030000234582"/>
    <dbReference type="OMA" id="GTWIRFF"/>
    <dbReference type="OrthoDB" id="264532at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006640">
        <property type="term" value="P:monoacylglycerol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd07987">
        <property type="entry name" value="LPLAT_MGAT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007130">
        <property type="entry name" value="DAGAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12317:SF19">
        <property type="entry name" value="DIACYLGLYCEROL O-ACYLTRANSFERASE 2-LIKE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03982">
        <property type="entry name" value="DAGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6 11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005012817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="337" mass="38139" checksum="07136B78B043FBDD" modified="2022-01-19" version="1">MAFLSRLDLQETLQTLSVLQWIPVYVFLGAVPILLIPYFLVFTKFWTLSVLALAWLAYDWNTHSQGGRRSAWVRNWTLWKYFGNYFPIKLVKTQDLSPKHNYIIASHPHGIVSYGVFINFATEATGFARIFPGITPSVGTLEGIFWIPIVREYVMSMGVCPVSESALKYLLTQKGSGNAVVVVVGGAAEALLCQPGVSTLVLKQRKGFVKLALTTGAYLVPSYSFGENEVYNQETFPEGTWIRFFQKTFQDTFKKILGLNFCTFHGRGLTRGSWGFLPFNRPITTVVGEPLPIPRIKKPNKKTVDKYHALYISALRKLFEQHKVHYGLPETQELTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PL14</accession>
    <name>A0A8C4PL14_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynein regulatory complex protein 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">IQ domain-containing protein D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">IQCD</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the nexin-dynein regulatory complex (N-DRC), a key regulator of ciliary/flagellar motility which maintains the alignment and integrity of the distal axoneme and regulates microtubule sliding in motile axonemes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the nexin-dynein regulatory complex (N-DRC). Interacts with CFAP52.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DRC10 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014714116.1">
        <property type="nucleotide sequence ID" value="XM_014858630.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PL14"/>
    <dbReference type="Ensembl" id="ENSEAST00005014766.2">
        <property type="protein sequence ID" value="ENSEASP00005013597.1"/>
        <property type="gene ID" value="ENSEASG00005009492.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106842245"/>
    <dbReference type="KEGG" id="eai:106842245"/>
    <dbReference type="CTD" id="75732"/>
    <dbReference type="GeneTree" id="ENSGT00730000111354"/>
    <dbReference type="OMA" id="AKIQKYW"/>
    <dbReference type="OrthoDB" id="536093at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd23767">
        <property type="entry name" value="IQCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042815">
        <property type="entry name" value="DRC10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000048">
        <property type="entry name" value="IQ_motif_EF-hand-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31598:SF1">
        <property type="entry name" value="DYNEIN REGULATORY COMPLEX PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31598">
        <property type="entry name" value="IQ DOMAIN-CONTAINING PROTEIN D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00612">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00015">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50096">
        <property type="entry name" value="IQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="7" id="KW-0969">Cilium</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="422"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="212"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="341"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="422"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="434"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005013597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="458" mass="53625" checksum="43AB9AA5C160DAA4" modified="2022-01-19" version="1">MALDVLTVEPLHQGPDINRIRLMTEPSKKPASPPNLLVPSSTKLTTIEAKRIMSVLDETIHKVQLVTLLSHVASGNKDLERMLGEGIMKAVREHEELCQLLLDNVSYLQRKERQVQEEEELEEERVLWDRRLSIELQKSALLPLKRQVRESTKNTLRLLLSNPQAASLLQTQTLGRRAGAQRLVDSLMELRGFLFEKLLTSPMEARDKAQFIQDIRKQNRRNQETINDLERELAASMKNRDAEVEKENFVIQELKNHLQQVLRFSDSSLLRTKQEAEKQQKADFRASQARVAKIQQELLLLRSQFHSLVMENREAEQALRKKKYKVETEIENWIQKYDMDMGEKQDEYEELDSVYKEEKAQLEELKQKHDVLMEEFNQIRAEQEINSRKRMEAEQELVRMVRAATVIQAVWKGYLVRSLLRSKRRKRSKSKAKDKKEKGKKKDKDKDKKGKEKGKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PM93</accession>
    <name>A0A8C4PM93_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aspartate aminotransferase</fullName>
            <ecNumber evidence="13">2.6.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">GOT2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:21824"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="EC" id="2.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4 13">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="13">In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.1" evidence="13"/>
    <dbReference type="RefSeq" id="XP_014682802.3">
        <property type="nucleotide sequence ID" value="XM_014827316.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PM93"/>
    <dbReference type="Ensembl" id="ENSEAST00005016947.2">
        <property type="protein sequence ID" value="ENSEASP00005015580.1"/>
        <property type="gene ID" value="ENSEASG00005010855.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822221"/>
    <dbReference type="KEGG" id="eai:106822221"/>
    <dbReference type="CTD" id="2806"/>
    <dbReference type="GeneTree" id="ENSGT00950000183082"/>
    <dbReference type="OMA" id="VGACTIV"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004069">
        <property type="term" value="F:L-aspartate:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006532">
        <property type="term" value="P:aspartate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015908">
        <property type="term" value="P:fatty acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006533">
        <property type="term" value="P:L-aspartate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019550">
        <property type="term" value="P:L-glutamate catabolic process to aspartate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043490">
        <property type="term" value="P:malate-aspartate shuttle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045471">
        <property type="term" value="P:response to ethanol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000026">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000001">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000160">
        <property type="entry name" value="Similar to aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000796">
        <property type="entry name" value="Asp_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006719">
        <property type="entry name" value="PRK09257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11879:SF22">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00799">
        <property type="entry name" value="TRANSAMINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 13" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0944">Nitration</keyword>
    <keyword evidence="7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="14">
        <location>
            <begin position="58"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005015580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="430" mass="47351" checksum="3D9238D90D44FBEF" modified="2022-01-19" version="1">MALLHSGRVLSGVAAAFHPGLAAAASARASSWWAHVEMGPPDPILGVTEAYKRDTNSKKMNLGVGAYRDDNGKPYVLPSVRKAEAQIAAKNLDKEYLPIGGLAEFCKASAELALGENNEALKSGRYVTVQSISGTGALRIGANFLQRFFKFSRDVFLPKPSWGNHTPIFRDAGLQLHAYRYYDPKTCGFDVTGALEDISKIPQQSIILLHACAHNPTGVDPRPEQWKEIATLVKKNNLFAFFDMAYQGFASGDGDKDAWAVRYFIEQGINVCLCQSYAKNMGLYGERVGAFTMVCKDADEAKRVESQLKILIRPLYSNPPLNGARIASTILTSPDLRKQWLQEVKGMADRIISMRTQLVSNLKKEGSSHSWQHIADQIGMFCFTGLKPEQVERLTKEFSIYMTKDGRISVAGVTSGNVGYLAHAIHQVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PNV9</accession>
    <name>A0A8C4PNV9_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="23">WT1 transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">WT1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the EGR C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PNV9"/>
    <dbReference type="Ensembl" id="ENSEAST00005021322.2">
        <property type="protein sequence ID" value="ENSEASP00005019655.2"/>
        <property type="gene ID" value="ENSEASG00005013477.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156734"/>
    <dbReference type="Proteomes" id="UP000694387">
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:adrenal gland development"/>
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        <property type="term" value="P:branching involved in ureteric bud morphogenesis"/>
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        <property type="term" value="P:cellular response to cAMP"/>
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        <property type="term" value="P:cellular response to gonadotropin stimulus"/>
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        <property type="term" value="P:glomerular basement membrane development"/>
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        <property type="term" value="P:male gonad development"/>
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    <dbReference type="GO" id="GO:0072207">
        <property type="term" value="P:metanephric epithelium development"/>
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        <property type="term" value="P:metanephric S-shaped body morphogenesis"/>
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        <property type="entry name" value="Krueppel-like factor 15"/>
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        <property type="entry name" value="Zinc finger protein 41"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
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        <property type="entry name" value="Wilms_tumour_N"/>
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        <property type="entry name" value="WILMS TUMOR PROTEIN"/>
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        <property type="entry name" value="WT1"/>
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        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
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    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
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    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    <keyword evidence="16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0691">RNA editing</keyword>
    <keyword evidence="14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="15" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
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    <sequence length="423" mass="47399" checksum="E3C464A4E02758D1" modified="2023-09-13" version="2">PRPAPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGKLRQRTVHCHGGKGRCSVRHTPKEADFSAWAWGGGPHSSGSDHWVHGYSTVTFDGTPSYGHAPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGHGAGYESDSHTTPILCGAQYRIHTHGVFRGIQDVRRVPGVTPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMSKLQLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PR47</accession>
    <name>A0A8C4PR47_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC123282544</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PR47"/>
    <dbReference type="Ensembl" id="ENSEAST00005025850.2">
        <property type="protein sequence ID" value="ENSEASP00005023829.2"/>
        <property type="gene ID" value="ENSEASG00005016186.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161825"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000349">
        <property type="entry name" value="Heat shock transcription factor, Y-linked"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF140">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR, X-LINKED MEMBER 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
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    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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            <begin position="80"/>
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            <begin position="10"/>
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            <begin position="190"/>
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            <begin position="240"/>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="361" mass="40172" checksum="B43D97ADACDF4776" modified="2023-09-13" version="2">MASQGTDQTDEVKQAPSGDREPATGVPTNSSLDPKLDSREILGTRSDQAINQDPGPQDNPRPQDPNQGIVHVEENHHLLGLSFPRKLWMVAEDDAFTSVRWNDEGDTVIIEEDLFQREILQRRGPGRIFETDSLKSFIRQLNLYGFSKIRPNDPSVHSPGNKRMMMYRNSNFQRNKPLLIENIRRRGNLRITTQPGASATTPKRKKQVEATRRSPRIHRNEPTKEGDRKAQKESPKAQGPSDTWSFVCSENHSVSSVAGHATENRHPSEPGSPSGEGTSRNVMFVPLATAGGDGAEEPPTSPPDYPDYDSVMSLYNTCYSILLASLSVMAPHEVPDEDEQQEGSSDYKCALCERFKDNPGP</sequence>
</entry>
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            <fullName evidence="9">Ret finger protein-like 4A</fullName>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="Sun"/>
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                <person name="J."/>
                <person name="Li"/>
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                <person name="Hou"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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    <reference evidence="7" key="3">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    </dbReference>
    <dbReference type="GO" id="GO:0005737">
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
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    </dbReference>
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        <property type="entry name" value="ConA-like_dom_sf"/>
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        <property type="entry name" value="TRIM/RBCC"/>
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        <property type="entry name" value="Znf_RING"/>
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        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="11"/>
            <end position="53"/>
        </location>
    </feature>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
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                <person name="Yang"/>
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                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Gao"/>
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                <person name="Li"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Yang"/>
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                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="19">Homodimer. Interacts (via extracellular domain) with CD6 (via extracellular domain). Homodimerization and interaction with CD6 involve the same region and cannot occur simultaneously. The affinity for CD6 is much higher than the affinity for self-association. Interacts (via glycosylated extracellular domain) with LGALS1 and LGALS3. Interaction with LGALS1 or LGALS3 inhibits interaction with CD6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="Ensembl" id="ENSEAST00005060126.1">
        <property type="protein sequence ID" value="ENSEASP00005037069.1"/>
        <property type="gene ID" value="ENSEASG00005011247.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106838873"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Guo"/>
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        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF160">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16497">
        <property type="entry name" value="MHC_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040363345" description="Ig-like domain-containing protein" evidence="9">
        <location>
            <begin position="19"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="185"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005037467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="339" mass="38404" checksum="27636C4AD9DECF17" modified="2023-09-13" version="1">MLFLQLHLLAVLLPGGDNKDDFQEPVSFQIIQISSFYNRSWVQNLGSGWLDELQTHGWESSSGTIIFLWPWSKGNYSNEEWMELEKLFRMSSIGIPQMFHNLASQWQLEYPFEVQITAGCELHFGEISVGFMRGAYQGSDFMSFQDNSWLPSPDGGSRAQHACELYNGDKVGLEITDRFLSDTCPRLLLSLLDAGKADLQRQVKPEAWLSTGPSPGPGRLMMVCHVSGFYPKPIWVMWMQGDQEHLGTQQSDILPNADGTWYLRKSLDVEALEAAGLSCRVRHSSLGGQDIIVYQERHSFTGWIFLAVIVPLVLLTGLAFWLRKRWTHCEAPCTIVPLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IBR9</accession>
    <name>A0A9L0IBR9_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IBR9"/>
    <dbReference type="Ensembl" id="ENSEAST00005074727.1">
        <property type="protein sequence ID" value="ENSEASP00005037661.1"/>
        <property type="gene ID" value="ENSEASG00005029661.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005037661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="95" mass="10540" checksum="A2892DA188EB3E86" modified="2023-09-13" version="1">MEHPLCARHCTSEQAVFPRLTVVLLAASKFFAAWFFVYRVTSTTDTQISTRSFSPLWWPCSLESSLESSSCCSGSASTYELPRVPSRGRTGSLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0ICH3</accession>
    <name>A0A9L0ICH3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Aminopeptidase NAALADL1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">N-acetylated-alpha-linked acidic dipeptidase-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">NAALADL1</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Aminopeptidase with broad substrate specificity. Has lower activity with substrates that have Asp or Glu in the P2' position, or Pro in the P3' position. Lacks activity with substrates that have both Pro in the P3' position and Asp or Glu in the P2' position. Lacks carboxypeptidase activity. Lacks dipeptidyl-peptidase IV type activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044618040.1">
        <property type="nucleotide sequence ID" value="XM_044762105.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0ICH3"/>
    <dbReference type="Ensembl" id="ENSEAST00005046796.1">
        <property type="protein sequence ID" value="ENSEASP00005038599.1"/>
        <property type="gene ID" value="ENSEASG00005018224.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106833668"/>
    <dbReference type="KEGG" id="eai:106833668"/>
    <dbReference type="CTD" id="10004"/>
    <dbReference type="GeneTree" id="ENSGT01030000234598"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004180">
        <property type="term" value="F:carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08022">
        <property type="entry name" value="M28_PSMA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02121">
        <property type="entry name" value="PA_GCPII_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000101">
        <property type="entry name" value="N-acetylated alpha-linked acidic dipeptidase like 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.30:FF:000021">
        <property type="entry name" value="N-acetylated alpha-linked acidic dipeptidase-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.930.40:FF:000001">
        <property type="entry name" value="N-acetylated-alpha-linked acidic dipeptidase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.40">
        <property type="entry name" value="Transferrin receptor-like, dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039373">
        <property type="entry name" value="Peptidase_M28B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007365">
        <property type="entry name" value="TFR-like_dimer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036757">
        <property type="entry name" value="TFR-like_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404:SF50">
        <property type="entry name" value="AMINOPEPTIDASE NAALADL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10404">
        <property type="entry name" value="N-ACETYLATED-ALPHA-LINKED ACIDIC DIPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF04253">
        <property type="entry name" value="TFR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47672">
        <property type="entry name" value="Transferrin receptor-like dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="12" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="16 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="7" id="KW-0645">Protease</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="8 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="22">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="23">
        <location>
            <begin position="166"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M28" evidence="25">
        <location>
            <begin position="353"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin receptor-like dimerisation" evidence="24">
        <location>
            <begin position="621"/>
            <end position="740"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
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            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
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            <dbReference type="ARBA" id="ARBA00022968"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023049"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00059290"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00068168"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00081462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF04253"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF04389"/>
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    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038599.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="745" mass="80280" checksum="B4355887BDCB6AFC" modified="2023-09-13" version="1">MQGVKVLAGVVGAAALLGLGIILGHFAIPKGADSPAPSISASQDLDLGILETIMEQLDASRIRENLRELSREPHLSSTPRDEELVQLLLQRWRDAESGLDSAETFEYEVLLSLPSQEQPNSVTVVDPTGNILFSCRRSEENVTGEQEGPDVVPPYAAYAPPGTPQGLLVYANQGSEEDFEELQNRGIGLEGTIALTRYGGVGRGAKAVNAAKHGVAGVLVYTDPSDINDGQSSPSQTFPYSWGLPPSGVERGSYLEYFGDPLTPYLPANPSSFRLDPDNASGFPPIPAQPIGFEDAKVLLCNLQGASAPETWQGALGCEYRLGPGFRSDGNFSADSQVNVSVHNRLELRNSSNVLGIIRGAVEPDRYVLYGNHRDSWVHGAVDPSSGTAVLLEVTRALGTLLKKGTWRPRRSVIFGSWGAEEFGLIGSTEFTEEFFSKLQERTVAYINVDISVFANVSLRAQGTPPIQSVVFTAAKQIPAPGRSSLSIYDNWIQYSNRSSPVYGLVPSLGTLGAGSDYAPFIHFLGISSMDLAYTYDRSKTSARIYPTYHTAFDTFGYVDNFVDPGFSSHQAVSRVVGNVLLRLSDSLFLPLNVSDYSETLRSFLQAAQQDLGALLEQHSISLGPLVAAVDKFEGAAAALDERISELQKGTPDPLQVRMLNDQLMLLERSFLNSRAFPEERYYSHVLWAPRTGSVATFPGLANACSAAMNTAPGSAAWAEVQRQLSIVVAAVEAAAATLRPVADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IGR4</accession>
    <name>A0A9L0IGR4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Caveolae associated protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">CAVIN3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <location evidence="1">Caveola</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CAVIN family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014693308.1">
        <property type="nucleotide sequence ID" value="XM_014837822.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IGR4"/>
    <dbReference type="SMR" id="A0A9L0IGR4"/>
    <dbReference type="Ensembl" id="ENSEAST00005051438.1">
        <property type="protein sequence ID" value="ENSEASP00005039382.1"/>
        <property type="gene ID" value="ENSEASG00005024523.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106828972"/>
    <dbReference type="KEGG" id="eai:106828972"/>
    <dbReference type="CTD" id="112464"/>
    <dbReference type="GeneTree" id="ENSGT00950000182910"/>
    <dbReference type="OrthoDB" id="9451657at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005901">
        <property type="term" value="C:caveola"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005080">
        <property type="term" value="F:protein kinase C binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032922">
        <property type="term" value="P:circadian regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901003">
        <property type="term" value="P:negative regulation of fermentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051898">
        <property type="term" value="P:negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026752">
        <property type="entry name" value="Cavin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15240:SF2">
        <property type="entry name" value="CAVEOLAE-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15240">
        <property type="entry name" value="CAVIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15237">
        <property type="entry name" value="PTRF_SDPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="140"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="201"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="261" mass="27411" checksum="E78E378E884A90E4" modified="2023-09-13" version="1">MGESALEPGPVPGAPAGGPVHAVTVVTLLEKLATMLEALQERQGGLARRQGGLAGSVRRIQSGLGALSRSHDTTSNTLAQLLAKAERVGSHADAAQERAVHRAAQVQRLEANHGLLVARGKLHVLLFKEEAEIPASAFQKAPEPLGPADQSEPGLEQPAVEVEESSDEEPVESRARRLRRTGLQKVQSLRRALSGRKGPAAPTPTPVKPPRLGPGRSAEGRSEAQPALESRQEPEPPQDTEEDPGRPGAAEAAVLQLESAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IH61</accession>
    <name>A0A9L0IH61_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Riboflavin transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SLC52A3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
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                <person name="J."/>
                <person name="Pan"/>
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                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Plasma membrane transporter mediating the uptake by cells of the water soluble vitamin B2/riboflavin that plays a key role in biochemical oxidation-reduction reactions of the carbohydrate, lipid, and amino acid metabolism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>riboflavin(in) = riboflavin(out)</text>
            <dbReference type="Rhea" id="RHEA:35015"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Cell membrane</location>
            <topology evidence="2 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the riboflavin transporter family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IH61"/>
    <dbReference type="Ensembl" id="ENSEAST00005070418.1">
        <property type="protein sequence ID" value="ENSEASP00005040228.1"/>
        <property type="gene ID" value="ENSEASG00005003605.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003774"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032217">
        <property type="term" value="F:riboflavin transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072388">
        <property type="term" value="P:flavin adenine dinucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006771">
        <property type="term" value="P:riboflavin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009357">
        <property type="entry name" value="Riboflavin_transptr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12929">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12929:SF4">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 52, RIBOFLAVIN TRANSPORTER, MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06237">
        <property type="entry name" value="SLC52_ribofla_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="72"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="105"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="137"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="293"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="333"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="360"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="399"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="428"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU368035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005040228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="469" mass="50804" checksum="658C6855BDA25442" modified="2023-09-13" version="1">MALLTHLLVCTFGMGSWVAINGLWVELPLLVMELPEGWFLPSYLTLVIQLANIGPLLVTLLHRFRPGCLSDVPVIFTLLGVGTVTCLLFAFLWNVTSLLQGKSYSIIFLVLTLFLALVDCTSSVTFLPFMSRLPTRYVTTFFVGEGLSGLLPALLALAQGSGLTTCSNVTDSSGTTPHPVTTRGTNTTQGDNSILMSTLPGTAPSVAQLETRYLPAHFSPLVYFLLLSFMMACCLIAFFFLQRQPRPWKSSTEDLLSSQVILHSIRLREEQEPGPPGLADSSRGQGHLEEKTAPLHLVHLAFIYILVAFVNALTNGVLPSVQTYSCLSYGPVAYHLSATLSSMANPLACFLSMFLPNRSLLFLGVLSVLGTGFGAYNMAMAVMSPCPLLWDHWGGEVLIVASWVLFAGCLSYVKVMLGTILRDRSRSALLWCGAAVQLGSLLGALLMFPLVNVLRLFSSGDSCSLECST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IH95</accession>
    <name>A0A9L0IH95_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Selenoprotein W</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SELENOW</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a role as a glutathione (GSH)-dependent antioxidant. May be involved in a redox-related process. May play a role in the myopathies of selenium deficiency.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with DPYSL2, PRDX1, YWHAB, YWHAG, HSP70 and HSP90.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the SelWTH family. Selenoprotein W subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IH95"/>
    <dbReference type="Ensembl" id="ENSEAST00005065459.1">
        <property type="protein sequence ID" value="ENSEASP00005039547.1"/>
        <property type="gene ID" value="ENSEASG00005004352.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161069"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000158">
        <property type="entry name" value="Selenoprotein W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011893">
        <property type="entry name" value="Selenoprotein_Rdx-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051441">
        <property type="entry name" value="SelW_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02174">
        <property type="entry name" value="CXXU_selWTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15124">
        <property type="entry name" value="SELENOPROTEIN W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15124:SF16">
        <property type="entry name" value="SELENOPROTEIN W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10262">
        <property type="entry name" value="Rdx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0318">Glutathionylation</keyword>
    <keyword evidence="6" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0712">Selenocysteine</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="184" mass="19163" checksum="952796DA5BEF3351" modified="2023-09-13" version="1">MLVGSPCRPPLSACAPSPSRLPCSVPVSASFLGSCLPISVRLPNHLCVGSLLSLLGVFLPSVLSCVPLLISSGPPYTPRLVSPLLFPDATCLHSLGLSPTSGAPIDPCGDLHPFQYLALKKKLEDEFPGRLDICGEGTPQVTGFFEVMVAGKLVHSKKGGDGYVDTEVKFLKLVAAIKAALAQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IHM0</accession>
    <name>A0A9L0IHM0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-rich sequence factor 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">GRSF1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences. Involved in the degradosome-mediated decay of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules. Acts by unwinding G-quadruplex RNA structures in MT-ncRNA, thus facilitating their degradation by the degradosome. G-quadruplexes (G4) are non-canonical 4 stranded structures formed by transcripts from the light strand of mtDNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Monomer. Found in a complex with DDX28, DHX30, FASTKD2 and FASTKD5. Interacts with the mitochondrial RNase P complex subunit TRMT10C/MRPP1. Interacts with the 2 components of the mitochondrial degradosome complex, PNPT1 and SUPV3L1, in an RNA-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IHM0"/>
    <dbReference type="Ensembl" id="ENSEAST00005050554.1">
        <property type="protein sequence ID" value="ENSEASP00005039672.1"/>
        <property type="gene ID" value="ENSEASG00005016685.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158529"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035770">
        <property type="term" value="C:ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016331">
        <property type="term" value="P:morphogenesis of embryonic epithelium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000962">
        <property type="term" value="P:positive regulation of mitochondrial RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12730">
        <property type="entry name" value="RRM1_GRSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12505">
        <property type="entry name" value="RRM2_GRSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12733">
        <property type="entry name" value="RRM3_GRSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000279">
        <property type="entry name" value="G-rich RNA sequence binding factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000071">
        <property type="entry name" value="heterogeneous nuclear ribonucleoprotein H isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000131">
        <property type="entry name" value="Heterogeneous nuclear ribonucleoprotein h3 isoform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050666">
        <property type="entry name" value="ESRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034424">
        <property type="entry name" value="GRSF-1_RRM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034425">
        <property type="entry name" value="GRSF1_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034426">
        <property type="entry name" value="GRSF1_RRM3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13976">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="261"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="361"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="14">
        <location>
            <begin position="512"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="11"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00062888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00073442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="591" mass="64657" checksum="8BD54FABD0382745" modified="2023-09-13" version="1">MCPHPLPALDSTSSPLALQQQPPPGPAPPPESMAGTRWVLGALLRGCGCNCSSCRRTGAACLPFYSAPGSFPSGVSGRRRLLLLLGAAAAAASQTRGLQTGPAPAGRLAGLSPAAASAAAAAAAASYPALRAPLLPQSLAAAAGPARELQPGMWEPRGAARGTLPRPRARAPAPLRVRLACAPGEPRHAPHPRPPAAQSSYWAPEQGTLCQPSGFLLVLFSSRVSGRWGSESKTTYLEDLPPLPEYEVAPSKLGQDVDDVYLIRAQGLPWSCTIEDVLNFFSDCRIRNGENGIHFLLNRDGKRRGDALIEMESEQDVQKALEKHRMYMGQRYVEVYEINNEDVDALMKSLQVKSSPVVNDGVVRLRGLPYSCNEKDIVDFFAGLNIVDITFVMDYRGRRKTGEAYVQFEEPEMASQALLKHREEIGNRYIEIFPSRRNEVRTHVGSHKGKKMASSPTAKYITEPEMVFEEHEVNEDIRPMTAFESEKEVELPKEMSEKLPETADFGTASSLHFVHMRGLPFQANAQDIINFFAPLKPVRITMEYSSSGKATGEADVHFDTHEDAVAAMLKDRSHVHHRYIELFLNSCPKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IJY1</accession>
    <name>A0A9L0IJY1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tumor necrosis factor ligand superfamily member 18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Glucocorticoid-induced TNF-related ligand</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">TNFSF18</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Cytokine that binds to TNFRSF18/AITR/GITR. Regulates T-cell responses. Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B. Triggers increased phosphorylation of STAT1 and up-regulates expression of VCAM1 and ICAM1. Promotes leukocyte adhesion to endothelial cells. Regulates migration of monocytes from the splenic reservoir to sites of inflammation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tumor necrosis factor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IJY1"/>
    <dbReference type="Ensembl" id="ENSEAST00005038427.1">
        <property type="protein sequence ID" value="ENSEASP00005041198.1"/>
        <property type="gene ID" value="ENSEASG00005036989.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002560"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045785">
        <property type="term" value="P:positive regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002687">
        <property type="term" value="P:positive regulation of leukocyte migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080090">
        <property type="term" value="P:regulation of primary metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042129">
        <property type="term" value="P:regulation of T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002309">
        <property type="term" value="P:T cell proliferation involved in immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033209">
        <property type="term" value="P:tumor necrosis factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000026">
        <property type="entry name" value="Tumor necrosis factor ligand superfamily member 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006052">
        <property type="entry name" value="TNF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042380">
        <property type="entry name" value="TNFSF18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15267">
        <property type="entry name" value="TUMOR NECROSIS FACTOR LIGAND SUPERFAMILY MEMBER 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15267:SF1">
        <property type="entry name" value="TUMOR NECROSIS FACTOR LIGAND SUPERFAMILY MEMBER 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50049">
        <property type="entry name" value="THD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0202">Cytokine</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0391">Immunity</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="THD" evidence="17">
        <location>
            <begin position="64"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00074590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00078193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005041198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="194" mass="21986" checksum="76B361F5504752FF" modified="2023-09-13" version="1">MAIFRLCPHTLSFPQRMSLNHMENMPLSHSSPQGAQRTSWKQWVLYSTIIILLLLCSFGTLILTFLPLKTANETCVAKFGPLPSKWQMTSPEPPCVNEIADWQLRILQNGLYLIYGQVDPNTAYEGAAPFEVRLYKNEDIIQTLTSNSEIQNVGGTYELHAGDTINLKFNSEHQVLKNNTYWGILLLANPQFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IK94</accession>
    <name>A0A9L0IK94_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14">Low molecular weight phosphotyrosine protein phosphatase</fullName>
            <shortName evidence="14">LMW-PTP</shortName>
            <shortName evidence="14">LMW-PTPase</shortName>
            <ecNumber evidence="3 14">3.1.3.2</ecNumber>
            <ecNumber evidence="4 14">3.1.3.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 14">Low molecular weight cytosolic acid phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ACP1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:10685"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:15018"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with EPHA2; dephosphorylates EPHA2. Interacts with EPHB1. Interacts with the SH3 domain of SPTAN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the low molecular weight phosphotyrosine protein phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.2" evidence="3 14"/>
    <dbReference type="EC" id="3.1.3.48" evidence="4 14"/>
    <dbReference type="RefSeq" id="XP_014697112.1">
        <property type="nucleotide sequence ID" value="XM_014841626.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IK94"/>
    <dbReference type="Ensembl" id="ENSEAST00005041816.1">
        <property type="protein sequence ID" value="ENSEASP00005037478.1"/>
        <property type="gene ID" value="ENSEASG00005022000.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831429"/>
    <dbReference type="KEGG" id="eai:106831429"/>
    <dbReference type="CTD" id="52"/>
    <dbReference type="GeneTree" id="ENSGT00940000158351"/>
    <dbReference type="OrthoDB" id="3388at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003993">
        <property type="term" value="F:acid phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004726">
        <property type="term" value="F:non-membrane spanning protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16343">
        <property type="entry name" value="LMWPTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000105">
        <property type="entry name" value="Low molecular weight phosphotyrosine protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050438">
        <property type="entry name" value="LMW_PTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023485">
        <property type="entry name" value="Ptyr_pPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036196">
        <property type="entry name" value="Ptyr_pPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002115">
        <property type="entry name" value="Tyr_Pase_low_mol_wt_mml"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017867">
        <property type="entry name" value="Tyr_phospatase_low_mol_wt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11717:SF34">
        <property type="entry name" value="LOW MOLECULAR WEIGHT PHOSPHOTYROSINE PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11717">
        <property type="entry name" value="LOW MOLECULAR WEIGHT PROTEIN TYROSINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01451">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00719">
        <property type="entry name" value="LMWPTPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00720">
        <property type="entry name" value="MAMMALPTPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00226">
        <property type="entry name" value="LMWPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52788">
        <property type="entry name" value="Phosphotyrosine protein phosphatases I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 14" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphotyrosine protein phosphatase I" evidence="15">
        <location>
            <begin position="7"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="13">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="active site" evidence="13">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00033695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00051341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00062457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR617867-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU368115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005037478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="158" mass="18134" checksum="72B4F480D6A529CA" modified="2023-09-13" version="1">MAEQVSKSVLFVCLGNICRSPIAEAVFRKLVTDQNISDNWRIDSAATSTYEIGNPPDYRGQSCMKRHGIPMSHTARQVTKEDFATFDYILCMDESNLRDLNRKSNQVKNCKAKIELLGSYDPQKQLIIEDPYYGNDSDFETVYQQCMRCCRAFLEKAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0ILH3</accession>
    <name>A0A9L0ILH3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106828440</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044614028.1">
        <property type="nucleotide sequence ID" value="XM_044758093.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0ILH3"/>
    <dbReference type="Ensembl" id="ENSEAST00005080549.1">
        <property type="protein sequence ID" value="ENSEASP00005041738.1"/>
        <property type="gene ID" value="ENSEASG00005034461.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="eai:106828440"/>
    <dbReference type="GeneTree" id="ENSGT01030000234540"/>
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        <property type="component" value="Chromosome 25"/>
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        <property type="term" value="C:membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="Immunoglobulins"/>
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        <property type="entry name" value="CD2/SLAM_rcpt"/>
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        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR12080">
        <property type="entry name" value="SIGNALING LYMPHOCYTIC ACTIVATION MOLECULE"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040327880" description="Ig-like domain-containing protein" evidence="7">
        <location>
            <begin position="29"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="258"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="8">
        <location>
            <begin position="158"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="292"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="316"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005041738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="349" mass="38414" checksum="3046DED93CAFAF0F" modified="2023-09-13" version="1">MGPCSEDPHLCWASWLLGFSSLLLNVCSTGFKSPGAHDAGGQDSGSYVSLKGIRGGSVWFHVTKESGTELEEIVWAFGPAIKYTVMLKVRKGSEETPTWVNLQDKYMQRVHVPNMTSLRIENLTREDSGQYQARANLPGGMESHQIFDLTVYEPVPLPQIQVQSVSTTPGWCNITLECRAAGVTEDLNVTWESKDLPRELEPARNSWTLAVSLPLSQHNASLTCVVSNSKDRKTVTSSLGKVCAHDSQGHASTGTLKAILGPIVAVPLILGVGLYLWKTHEKKKKMEIGRGAGLQEEHRNHDDDMQYAELSLQESRGGKDKSMGERCLQEKEPLTTVYSEVQKPGQARP</sequence>
</entry>
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        <recommendedName>
            <fullName evidence="4">Loricrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">LORICRIN</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0ILN4"/>
    <dbReference type="Ensembl" id="ENSEAST00005068886.1">
        <property type="protein sequence ID" value="ENSEASP00005041803.1"/>
        <property type="gene ID" value="ENSEASG00005028617.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001533">
        <property type="term" value="C:cornified envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030280">
        <property type="term" value="F:structural constituent of skin epidermis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031700">
        <property type="entry name" value="Loricrin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39228">
        <property type="entry name" value="LORICRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39228:SF1">
        <property type="entry name" value="LORICRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15847">
        <property type="entry name" value="Loricrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="273"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005041803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="322" mass="26907" checksum="1018B5CFB36922E8" modified="2023-09-13" version="1">MLSAFFPGFPPSLDKMSHQKKQPTPQPPVGCGKISGGGGGGGGGGGGGGGGGSGGCGFYSGGGGGGGGSGGCGGGSGGSSGGNIKYSGGGGSSCGGGSSGGGSSSCGGGSSGGSSGGGSGCYSSGGGSSGGGGSSGGSSGFYSGGGGGGGSSGQQVQCQSYGGISSGGSSGGDSSCGGGYSGGGGSSGGGSSCGGGYSGGGGSSCGGGSSGGGPGYFSSQQSTQTSCAPQPSYGGGSSGCSGSSGGGSGCFSSGGGGGGSGCGGGSSGGGSGCFSSGGGSSGGDGGCFSSGGGSSGGGSGSGKGVPVYHQTQQKQAPTWPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IPT9</accession>
    <name>A0A9L0IPT9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">RNF152</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RNF152 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_014714922.1">
        <property type="nucleotide sequence ID" value="XM_014859436.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044630748.1">
        <property type="nucleotide sequence ID" value="XM_044774813.2"/>
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        <property type="protein sequence ID" value="ENSEASP00005046021.1"/>
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        <property type="protein sequence ID" value="ENSEASP00005047694.1"/>
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    <keyword evidence="9 15" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="166"/>
            <end position="187"/>
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        <location>
            <begin position="12"/>
            <end position="55"/>
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        <source>
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    <sequence length="203" mass="22313" checksum="841EB09030302D37" modified="2023-09-13" version="1">METLSQDSLLECQICFNYYSPRRRPKLLDCKHTCCSVCLQQMRTSQKDVRCPWCRGITKLPPGFSVSQLPDDPEVLAVIAIPHASEHTPVFIKLPSNGCYMLPLPISKERALLPGDMGCRLLPGSQQKSVTVVTIPAEQQPLQGGAPQEAAEEEQDRRGVVKSSTWSGVCTVILVACVLVFLLGIVLHNMSCISKRFTVISCG</sequence>
</entry>
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    <accession>A0A9L0IRL0</accession>
    <name>A0A9L0IRL0_EQUAS</name>
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        <recommendedName>
            <fullName evidence="4">SCAN box domain-containing protein</fullName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IRL0"/>
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        <property type="gene ID" value="ENSEASG00005032159.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 2"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050916">
        <property type="entry name" value="SCAN-C2H2_zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935">
        <property type="entry name" value="PROTEIN ZBED8-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45935:SF26">
        <property type="entry name" value="SCAN DOMAIN-CONTAINING PROTEIN SCAND2P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SCAN box" evidence="4">
        <location>
            <begin position="47"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="175"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="183"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="196" mass="22308" checksum="0B9618781725142D" modified="2023-09-13" version="1">MAVSVDQEIPASQVQEELRIVKLEEDSAWVQETSLQRSNPGPETSCQRFWHFRYQEASGPREALIQLWTLCRQWLRPEKCTKEQILELLVLEQFLTVLPQELQIWVRQKHPESGEEAVGLVEDLQKEPGRQGLQAMVKNSQPEPEEQLNHDPGVEVLCFHKSALPDACALGLPVEESSGDQRMMSSSQISRPTDPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IU23</accession>
    <name>A0A9L0IU23_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">M-phase phosphoprotein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">MPHOSPH8</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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                <person name="Zhao"/>
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                <person name="Wang"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Guo"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Liu"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
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        <scope>IDENTIFICATION</scope>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Equus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="6">Non-catalytic component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy. The C9orf72-SMCR8 complex promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB8A and RAB39B into their active GTP-bound form, thereby promoting autophagosome maturation. As part of the C9orf72-SMCR8 complex, stimulates RAB8A and RAB11A GTPase activity in vitro, however WDR42 is shown not be an essential complex component for this function. The C9orf72-SMCR8 complex also acts as a negative regulator of autophagy initiation by interacting with the ULK1/ATG1 kinase complex and inhibiting its protein kinase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the C9orf72-SMCR8 complex, at least composed of C9orf72, SMCR8 and WDR41. The complex is formed of two protomers, each individually consisting of one molecule each of C9orf72, SMCR8 and WDR41. The protomers homodimerize via an interaction between C9orf72 (via C-terminus) and SMCR8 (via N-terminus). Within each protomer SMCR8 (via DENN domain) acts as a bridging protein between WDR41 (via C-terminus and N-terminus) and C9orf72 (via C-terminus). The C9orf72-SMCR8 complex associates with the ULK1/ATG1 kinase complex.</text>
    </comment>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
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        <property type="project" value="Ensembl"/>
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        <location>
            <begin position="147"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="564" mass="64037" checksum="048341258E1CB441" modified="2023-09-13" version="1">MVIPWTLPKRRDERVDRDLFSCRKQSVTFSWIDWIQMAPVPRVTLARQLDSSLEASVGQCQHIYSVAKVMPSVYDHMGEWKEHSEVFLDSADLWHSGPEREGIRVLCSLLNSMRRHPLLELEPNNKSPLQTIGEEQTQNPYTELLVLRAHRDIVRFLVQLDDCRFASAGDDGIVVVWNAQTGEKLLELSGHTQKITAIITFPPLEACEEKNQLILTASADKTVIVWDCDTGRQVRKVSCFHSTVKCLTILQRLDVWLSGGSDLCVWNRRLALLCKTSHLSDTGVSALIEIPKNCVVAAVGKELIIFKLVAPTEGSLEWNILEVKRLLDHQDNILSLVNVNDLSFVSGSHVGELIIWDVSDWTVQACERNFCTQLDTQQEIKLYQKPNDISIHHFTWDEENVFAAVGRGLYVYNLQMKRVIACQKTAHDSSVLHIARLPNRQLISCSEDGSVRIWELREKQQLAAEPVPTGFFNMWGFGRVNKQASQPVKKQQENTTPCSLELIGDLIGHSSSVEMFLYFEDHGLVTCSADHLIILWKNGERESGLRSLKLFQKLEENGDLYLTV</sequence>
</entry>
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    <name>A0A9L0IYQ8_EQUAS</name>
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        <submittedName>
            <fullName evidence="4">TNF superfamily member 9</fullName>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the tumor necrosis factor family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014843638.3"/>
    </dbReference>
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    <dbReference type="Ensembl" id="ENSEAST00005074505.1">
        <property type="protein sequence ID" value="ENSEASP00005045263.1"/>
        <property type="gene ID" value="ENSEASG00005028605.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106832762"/>
    <dbReference type="KEGG" id="eai:106832762"/>
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    <dbReference type="GeneTree" id="ENSGT00390000006244"/>
    <dbReference type="OMA" id="GPLRWYS"/>
    <dbReference type="OrthoDB" id="9450706at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005164">
        <property type="term" value="F:tumor necrosis factor receptor binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042104">
        <property type="term" value="P:positive regulation of activated T cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045585">
        <property type="term" value="P:positive regulation of cytotoxic T cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00184">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.40:FF:000032">
        <property type="entry name" value="Tumor necrosis factor ligand superfamily member 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021184">
        <property type="entry name" value="TNF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006052">
        <property type="entry name" value="TNF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042373">
        <property type="entry name" value="TNFSF9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15153">
        <property type="entry name" value="TUMOR NECROSIS FACTOR LIGAND SUPERFAMILY MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15153:SF0">
        <property type="entry name" value="TUMOR NECROSIS FACTOR LIGAND SUPERFAMILY MEMBER 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00229">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00207">
        <property type="entry name" value="TNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49842">
        <property type="entry name" value="TNF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00251">
        <property type="entry name" value="THD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50049">
        <property type="entry name" value="THD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="92"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005045263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="256" mass="26089" checksum="D1815FACB5088EA5" modified="2023-09-13" version="1">MVSNTSANPDPEAQRPPAPPGSAWRSLPWAVSAALLLFAGACAFCAVRAWALPGAPASSGPSPGPAPSSRLPEVPELPPDASARLPNTLQGVFAQLVAQDVRLTKGLLRWHSDPGLAGVSLAPGLSYDESTQELVVAEAGVYYVFLHLELKPVVASAGSGSVSAALRLKPPRTGAAALAMTLDLPPPSSGARDSRAVGFRGSLLHLGAGQRLSVHLLSVHLPAAAGAPPTWQLAQGATFLGLFRVAPAGLPSPLPT</sequence>
</entry>
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        <recommendedName>
            <fullName evidence="9">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GNGT2</name>
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    <organism evidence="12 13">
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">G proteins are composed of 3 units; alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Lipid-anchor</topology>
            <orientation evidence="1 9">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the G protein gamma family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A9L0IYY4"/>
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        <property type="protein sequence ID" value="ENSEASP00005042285.1"/>
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        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT"/>
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        <property type="entry name" value="G-gamma"/>
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        <property type="entry name" value="GPROTEING"/>
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    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
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        <property type="entry name" value="G_PROTEIN_GAMMA"/>
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    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
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    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="8" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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    <feature type="domain" description="G protein gamma" evidence="11">
        <location>
            <begin position="94"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="140"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="146"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004973"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50058"/>
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            <dbReference type="Ensembl" id="ENSEASP00005042285.1"/>
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    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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            <fullName evidence="3">Ig-like domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Yang"/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
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                <person name="Pan"/>
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                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_V-set"/>
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    <dbReference type="InterPro" id="IPR050413">
        <property type="entry name" value="TCR_beta_variable"/>
    </dbReference>
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        <property type="entry name" value="IMMUNOGLOBULIN V-SET DOMAIN-CONTAINING PROTEIN"/>
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        <property type="entry name" value="T-CELL RECEPTOR BETA CHAIN"/>
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    <keyword evidence="1" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="19"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005045598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="144" mass="16120" checksum="16AB5B99A742F76B" modified="2023-09-13" version="1">MGHEEAVEKRPPAIAVPEPAMSLRLLCCVALFFWGAGSMDTKVTQRPGHLVKGKEQKAKMTCVPIKSHSYVYWYRRKLEGEFEFLVYLQNNDVINKIEGFDQQFSAQCPKNASCSLEIKSTEPGDSALYFCASSESTVLNVSFS</sequence>
</entry>
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    <accession>A0A9L0IZV1</accession>
    <name>A0A9L0IZV1_EQUAS</name>
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        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
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        <name evidence="14" type="primary">LOC106842312</name>
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    <organism evidence="14 15">
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            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Zhao"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
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                <person name="Zhuang"/>
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                <person name="Gao"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
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                <person name="Li"/>
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                <person name="Hou"/>
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                <person name="Yang"/>
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                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cell membrane</location>
            <topology evidence="2 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IZV1"/>
    <dbReference type="Ensembl" id="ENSEAST00005082122.1">
        <property type="protein sequence ID" value="ENSEASP00005042585.1"/>
        <property type="gene ID" value="ENSEASG00005032160.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 1"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15420">
        <property type="entry name" value="7tmA_OR2A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000008">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
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                <person name="Q."/>
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                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1029"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1085"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1281"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1557"/>
            <end position="1570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1871"/>
            <end position="1883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00073415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005046327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1959" mass="204743" checksum="72D2193DD9C1E723" modified="2023-09-13" version="1">MSHPSPTAHKDLWVKKLCKPTFINLLTSPQSIIHSSQDLEEPRCPSKDEWIKMWEKEQETQKEERLMEEKKKKKQEEKKKKEGAQKKAADQKTKVPEPTKTCSSQPQPAGPSASTSTSTLSNSSNGKRAPASSQQPAASRYLPREVPPRFRQQEQKQLLKRGQPLPTGALTSVSPSQSAGPAGVSPPPIPGAGAQQHPSKLQPDLSHSGLADHYETSHWGQQPTYRSEATRSWDKVTIDRTDKEAWPSITGTETESASECTTDTDSASNCGSENSSMATGSAQGNFTGHTKKTNGNNGTNGALVQSPSNQSALGAGGANGNGSAARVWGVAAGSSSGLPHCSLSGGDGKMDNMIGDGRSQNCWGASNSNAGINLNLNPNANPAAWPVLGHEGAVATGNSSSICSPVSAVGQNMGNHNGNPAGTLGAWGNLLPQESTEPQTSTSQNVSFSVQPQNLNTDGPNNTKPMNPPPNPSNAMQANGLPNWGMAVGMGAIIPPHLQGLPGANGSSASQVSGGGGEGIGSSVWGLSPANPATGNSNSGFNQGNGDTVNSALSAKQNGSSSAVQKEGNGGNAWDSGPPAGPGILAWGRGSGNNAVGNIHSGAWGHPSRSTSTSVNGEWGKPPNQHSNSDINGKGSTGWDSPSVSSQNPAMQPGSEHMNSWAKAASSGTTASEGSSDGSGSHNEGSTGREGAEGRRRDKGIVDQGHIQLPRNDLDPRVLSNTGWGQTPVKQNTAWEFEESPRSERKNDNGTEAWGCAATQPSNSGGKNDGSIMNSTNTSSVSGWVSSPPAAVPANTGWGDSSNKAPNGPGVWGDSISSTAVNNAAAAKSGHAWSGTINQEDKSPTWGEPQKSKSQNWGDGQKANPAWSAGAGDWADSSSVLGHLGDGKKAGSAWDADSNRSGSGWNDATRSGTSGWGNGTNAKVNPGTNWGESLKPGPQQNWASKPQDNNMGNWGGAASVKQTGAGWIGGPVPVKQKDNGEATGWEEPSPPSIRRKMEIDDGTSAWGDPSSYTNKTVNMWDRNNPGLQNSTTANSTTTTTTTSNSTNVGETPPSHQASAPLNRSPLLGPVSSGWGEMPNVHSKPESSWGEPSSPSTLVDNGTAAWGKPPSSGSGWGDQPAEPAVTFGRAGAPAAAPALCKPASKSMQEGWGSGGDDMNLGTGQWEEEEGSMWNNAASQESTSSCSSWGNASKKGLQKGVKTSGKQDEAWIMNRLIRQLTDMGFPREPAEEALKSNNMNLDQAMSALLEKKVDMDKRGLGVPDYNGMVTKPLGCRPPISKESSVDRPTFLDKDGGLVEEPTTSPFLPSPSLKLPLSNSALPNQALGGIASGLGMQNLNSSRQIPSGNLGVFGSSGAAQARTMQQPPQPPVQPLNSSQPSLRAQVPQFLSPQVQAQLLQFAAKNIGLNPALLTSPINPQHMTLLNQLYQLQLAYQRLQIQQQMLQAQRNVSGPMRQQEQQVARTITNLQQQIQQHQRQLAQALLVKPPPPPPHLSLHPSAGKSAVDSFPPHPQAPGLPDLQTKEQQPSPSTFAPHPLAGLNPNMNVNSMDMTGGLSVKDPSQSQSRLPQWTHPSPMDSVPGAASPLLDQNPSKHGAIPGGLGIGPPAKSSIDDSYGRYDLIQNSESPASPPVAVPHSWSRAKSDSDKISNGSSINWPPEFHPGVPWKGLQNIDPENDPDVTPGSVPTGPTINTTIQDVNRYLLKSGGKLSDIKSTWSSGPASHTQASLSHELWKVPRNTAAPTRPPPGLTTPKPSSTWGASPLGWTSSYSSGSAWSTDTSGRTSSWLVLRNLTPQIDGSTLRTLCLQHGPLITFHLNLTQGNAVVRYSSKEEAAKAQKSLHMCVLGNTTILAEFAGEEEVNRFLAQGQALPPTSSWQSSTGTSQTRLGASGSSHGLVRSDAGHWNAPCLAGKGSSDLLWGGVPQYSSSLWGPPSSDDGRVIGSPTPLNTLLPGDLLSGESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J8I1</accession>
    <name>A0A9L0J8I1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">G protein-coupled receptor 176</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">GPR176</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Orphan receptor involved in normal circadian rhythm behavior. Acts through the G-protein subclass G(z)-alpha and has an agonist-independent basal activity to repress cAMP production.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014702822.1">
        <property type="nucleotide sequence ID" value="XM_014847336.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J8I1"/>
    <dbReference type="Ensembl" id="ENSEAST00005045137.1">
        <property type="protein sequence ID" value="ENSEASP00005049601.1"/>
        <property type="gene ID" value="ENSEASG00005034823.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106835165"/>
    <dbReference type="KEGG" id="eai:106835165"/>
    <dbReference type="CTD" id="11245"/>
    <dbReference type="GeneTree" id="ENSGT00950000182998"/>
    <dbReference type="OMA" id="VTDIYAM"/>
    <dbReference type="OrthoDB" id="9339792at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007193">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048512">
        <property type="term" value="P:circadian behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd15006">
        <property type="entry name" value="7tmA_GPR176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000220">
        <property type="entry name" value="Probable G-protein coupled receptor 176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043523">
        <property type="entry name" value="GPCR_176_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22752:SF1">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 176"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0090">Biological rhythms</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="49"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="122"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="203"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="300"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="58"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="469"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005049601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="515" mass="56878" checksum="37D5A01ACEDEA00F" modified="2023-09-13" version="1">MGHNGSWISRNASEPRNASGAEAAGANRSALGEFGEAQLYRQFTTTVQVVIFIGSLLGNFMVLWSTCRTTVFKSVTNRFIKNLACSGICASLVCVPFDIVLSASPHCCWWIHTMLFCKVVKFLHKVFCSVTILSFPAIALDRYYSVLYPLERKISDAKSRELVMYIWAHAVVASVPVFAMTNVADIYAMSTCTEVWSNSLGHLVYVLIYNITTVIVPVAVVFLFLILIRRALSASQKKKVIIAALRTPQNTISIPYASQREAELHATLLSMVMVFILCSVPYATLVVYQTVLNVPDTSIFLLLTAIWLPKVSLLANPVLFLTVNKSVRKCLVGTLVQLHHRYSRRNVVSTGGGMADASLEPSMRSGSQLLEMFHIGQQQIFKPTEDEEESEAKYTGSADLQAKEILTGCLEGEQGPQFAPPAPPPGTVDSVSQVAAAASVEPETFPEKYSLQFGFGPFELPPQWLSETRNSKKRLLPPLGNTPEELIQTKMPKVGRVERKMSRNNKVSIFPKVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JA79</accession>
    <name>A0A9L0JA79_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vesicle transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SFT2D2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1 9">May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex.</text>
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        <subcellularLocation>
            <location evidence="2 9">Membrane</location>
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        <text evidence="8 9">Belongs to the SFT2 family.</text>
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        <property type="nucleotide sequence ID" value="XM_044757958.2"/>
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        <property type="protein sequence ID" value="ENSEASP00005049278.1"/>
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        <property type="component" value="Chromosome 25"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="term" value="P:protein transport"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
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        <property type="entry name" value="Vesicle_transpt_SFT2"/>
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    <dbReference type="PANTHER" id="PTHR23137:SF1">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN SFT2B"/>
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        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
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        <property type="entry name" value="Got1"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="125"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00003566"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005049278.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="X. and Zhong"/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="gene ID" value="ENSEASG00005028722.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome Y"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005048330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="39" mass="4558" checksum="3D48817FB8F3BF66" modified="2023-09-13" version="1">MAEHHAQNKARLISETRRRFEAEYVTGGYRVLRVEAGVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JHF9</accession>
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        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
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        </recommendedName>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
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                <person name="Wang"/>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
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                <person name="S."/>
                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Gao"/>
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                <person name="Ju"/>
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                <person name="Li"/>
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                <person name="R."/>
                <person name="Hou"/>
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                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0JHF9"/>
    <dbReference type="Ensembl" id="ENSEAST00005054461.1">
        <property type="protein sequence ID" value="ENSEASP00005052771.1"/>
        <property type="gene ID" value="ENSEASG00005026069.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158979"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
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    <dbReference type="GO" id="GO:0016020">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050305">
        <property type="entry name" value="Small_GTPase_Rab"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47980">
        <property type="entry name" value="LD44762P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0636">Prenylation</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="112"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="137" mass="15237" checksum="F5F5C33F7E4DEB5D" modified="2023-09-13" version="1">NQTDGSPLSRTSTDYLLKFLLAGDSDVGKGKILQDGAAESPYAYSNWIDYETTTILPDGQRVRLLLWDAPGWARLCTRFRSYFRSAQGLLLLYDIINGWFFGRAKASKGPLPSTIHPIPSQPQPPPAQNCSRSNCKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JHK3</accession>
    <name>A0A9L0JHK3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B</fullName>
            <ecNumber evidence="6">2.4.99.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">STT3B</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a di-trans,poly-cis-dolichyl diphosphooligosaccharide + L-asparaginyl-[protein] = N(4)-(oligosaccharide-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl-(1-&gt;4)-N-acetyl-beta-D-glucosaminyl)-L-asparaginyl-[protein] + a di-trans,poly-cis-dolichyl diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22980"/>
            <dbReference type="Rhea" id="RHEA-COMP:12804"/>
            <dbReference type="Rhea" id="RHEA-COMP:12805"/>
            <dbReference type="Rhea" id="RHEA-COMP:19506"/>
            <dbReference type="Rhea" id="RHEA-COMP:19509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:50347"/>
            <dbReference type="ChEBI" id="CHEBI:57497"/>
            <dbReference type="ChEBI" id="CHEBI:57570"/>
            <dbReference type="ChEBI" id="CHEBI:132529"/>
            <dbReference type="EC" id="2.4.99.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Component of the oligosaccharyltransferase (OST) complex. There are 2 OST complexes, OST-A and OST-B, which contain STT3A or STT3B as catalytic subunit, respectively. OST-A and OST-B contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, and OST-B contains either MAGT1 or TUSC3 as specific accessory subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.18" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0JHK3"/>
    <dbReference type="Ensembl" id="ENSEAST00005041199.1">
        <property type="protein sequence ID" value="ENSEASP00005052816.1"/>
        <property type="gene ID" value="ENSEASG00005006174.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106830999"/>
    <dbReference type="KEGG" id="eai:106830999"/>
    <dbReference type="CTD" id="201595"/>
    <dbReference type="GeneTree" id="ENSGT00940000155488"/>
    <dbReference type="OMA" id="TKELWSP"/>
    <dbReference type="OrthoDB" id="10261066at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160227">
        <property type="term" value="C:oligosaccharyltransferase complex B"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004579">
        <property type="term" value="F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006516">
        <property type="term" value="P:glycoprotein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043687">
        <property type="term" value="P:post-translational protein modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006986">
        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12610:FF:000001">
        <property type="entry name" value="Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048999">
        <property type="entry name" value="STT3-PglB_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048307">
        <property type="entry name" value="STT3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872:SF1">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21436">
        <property type="entry name" value="STT3-PglB_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16" id="KW-0464">Manganese</keyword>
    <keyword evidence="14 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="263"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="322"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="440"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyl transferase STT3 N-terminal" evidence="22">
        <location>
            <begin position="72"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="domain" description="STT3/PglB/AglB core" evidence="23">
        <location>
            <begin position="601"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="490"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00065008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00070525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF02516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF21436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="826" mass="93685" checksum="4E47344341D199FF" modified="2023-09-13" version="1">MAEPSAPESKHKSSLNSSPWSGLMALGNSRHGHHGPGAQCAHKAAGGVAPPKPAPAGLSGGLSQPAGWQSLLSFTILFLAWLAGFSSRLFAVIRFESIIHEFDPWFNYRSTHHLASHGFYEFLNWFDERAWYPLGRIVGGTVYPGLMITAGLIHWILNTLNITVHIRDVCVFLAPTFSGLTSISTFLLTRELWNQGAGLLAACFIAIVPGYISRSVAGSFDNEGIAIFALQFTYYLWVKSVKTGSVFWTMCCCLSYFYMVSAWGGYVFIINLIPLHVFVLLLMQRYSKRVYIAYSTFYIVGLILSMQIPFVGFQPIRTSEHMAAAGVFALLQAYAFLQYLRDRLTKQEFQTLFFLGVSLAAGAVFLSVIYLTYTGYIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQPTTWVSFFFDLHILVCTFPAGLWFCIKNINDERVFVALYAISAVYFAGVMVRLMLTLTPVVCMLSAIAFSNVFEHYLGDDMKRENPPVEDSSDEDDKRNPGNLYDKAGKVRKHVTEQEKTEEGLGPNIKSIVTMLMLMLLMMFAVHCTWVTSNAYSSPSVVLASYNHDGTRNILDDFREAYFWLRQNTDEHARVMSWWDYGYQIAGMANRTTLVDNNTWNNSHIALVGKAMSSNETAAYKIMRSLDVDYVLVIFGGVIGYSGDDINKFLWMVRIAEGEHPKDIRESDYFTPQGEFRVDKAGSPTLLNCLMYKMSYYRFGEMQLDFRTPPGFDRTRNAEIGNKDIKFKHLEEAFTSEHWLVRIYKVKAPDNRETLDHKPRVTNIFPKQKYLSKKTTKRKRGYIKNKLVFKKGKKISKKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JHV6</accession>
    <name>A0A9L0JHV6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ubiquitin D</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Diubiquitin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ubiquitin-like protein FAT10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">UBD</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="J."/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">As part of the MCIA complex, involved in the assembly of the mitochondrial complex I.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the mitochondrial complex I assembly/MCIA complex that comprises at least the core subunits TMEM126B, NDUFAF1, ECSIT and ACAD9 and complement subunits such as COA1 and TMEM186. Interacts with ECSIT. Interacts with ACAD9. At early stages of complex I assembly, it is found in intermediate subcomplexes that contain different subunits including NDUFB6, NDUFA6, NDUFA9, NDUFS3, NDUFS7, ND1, ND2 and ND3. Interacts with TMEM70 and TMEM242.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CIA30 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014712845.1">
        <property type="nucleotide sequence ID" value="XM_014857359.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070359546.1">
        <property type="nucleotide sequence ID" value="XM_070503445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JII4"/>
    <dbReference type="Ensembl" id="ENSEAST00005051913.1">
        <property type="protein sequence ID" value="ENSEASP00005053141.1"/>
        <property type="gene ID" value="ENSEASG00005016383.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106841358"/>
    <dbReference type="KEGG" id="eai:106841358"/>
    <dbReference type="CTD" id="51103"/>
    <dbReference type="GeneTree" id="ENSGT00390000007200"/>
    <dbReference type="OMA" id="KRTGYAN"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.430">
        <property type="entry name" value="Galactose-binding lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013857">
        <property type="entry name" value="NADH-UbQ_OxRdtase-assoc_prot30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039131">
        <property type="entry name" value="NDUFAF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13194">
        <property type="entry name" value="COMPLEX I INTERMEDIATE-ASSOCIATED PROTEIN 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13194:SF18">
        <property type="entry name" value="COMPLEX I INTERMEDIATE-ASSOCIATED PROTEIN 30, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08547">
        <property type="entry name" value="CIA30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase intermediate-associated protein 30" evidence="10">
        <location>
            <begin position="126"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005053141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="328" mass="37767" checksum="1C782F6B6A1B3420" modified="2023-09-13" version="1">MALVHKLLNGTCILRKCPKPSVALYPFLGTRFADYCSSSLQKPAAVPGKASSQRKTEGDLQGHHQRDVALDITCPEEKPEVSFDKAIKDELKNHFWRLKDDIVNRWIGPEGRPLHEVLLEQARVVWKFRGKEDLDKWIVTSDKTIGGRSEAFLKMGKNNQSALLYGTLSSDTPQDGESSRSGYCAMISRIPRGPFERKRSYDWSQFNTLYLRVRGDGRPWMVNIREDTDIIQRKNQIYSYFMFTRGGPYWQDVKIPFSKFFFSNQGRIRDAQYQLLLDKISSIGFTLADKVDGPFFLEIDFIGVFTDPAHTEEFAYENSPELNPRLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JKJ3</accession>
    <name>A0A9L0JKJ3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Serine/threonine-protein kinase 26</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Mammalian STE20-like protein kinase 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">STK26</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Serine/threonine-protein kinase that acts as a mediator of cell growth. Modulates apoptosis. In association with STK24 negatively regulates Golgi reorientation in polarized cell migration upon RHO activation. Phosphorylates ATG4B at 'Ser-383', thereby increasing autophagic flux. Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 30">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 30">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="21">Homodimer. Interacts with PDCD10. Interacts with GOLGA2. Interacts with CTTNBP2NL. Interacts with RIPOR1 (via C-terminus); this interaction occurs in a PDCD10-dependent and Rho-independent manner. Interacts with PDCD10; this interaction is required for the association of STK26 with RIPOR1. Part of the core of STRIPAK complexes composed of PP2A catalytic and scaffolding subunits, the striatins (PP2A regulatory subunits), the striatin-associated proteins MOB4, STRIP1 and STRIP2, PDCD10 and members of the STE20 kinases, such as STK24 and STK26.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="RefSeq" id="XP_014713954.1">
        <property type="nucleotide sequence ID" value="XM_014858468.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JKJ3"/>
    <dbReference type="Ensembl" id="ENSEAST00005065478.1">
        <property type="protein sequence ID" value="ENSEASP00005049705.1"/>
        <property type="gene ID" value="ENSEASG00005000327.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106842133"/>
    <dbReference type="KEGG" id="eai:106842133"/>
    <dbReference type="CTD" id="51765"/>
    <dbReference type="GeneTree" id="ENSGT00940000157904"/>
    <dbReference type="OMA" id="YYGCFLD"/>
    <dbReference type="OrthoDB" id="8693905at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030336">
        <property type="term" value="P:negative regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06640">
        <property type="entry name" value="STKc_MST4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000411">
        <property type="entry name" value="Probable Ste20-like kinase Don3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.70:FF:000004">
        <property type="entry name" value="Serine/threonine-protein kinase 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000252">
        <property type="entry name" value="Serine/threonine-protein kinase 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046409">
        <property type="entry name" value="PDC10_dimerisation_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048288">
        <property type="entry name" value="PDCD10_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035056">
        <property type="entry name" value="STK_MST4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF7">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </evidence>
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                <person name="Li"/>
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                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="3">Component of the large ribosomal subunit.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
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    <dbReference type="KEGG" id="eai:106838879"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="60S ribosomal protein l24"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023442">
        <property type="entry name" value="Ribosomal_eL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01073">
        <property type="entry name" value="RIBOSOMAL_L24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="TRASH" evidence="11">
        <location>
            <begin position="6"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="106"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="106"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00075501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005054408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="157" mass="17779" checksum="1D48EEB7C0652574" modified="2023-09-13" version="1">MKVELCSFSGYKIYPGHGRRYARTDGKVFQFLNAKCESAFLSKRNPRQINWTVLYRRKHKKGQSEEIQKKRTRRAVKFQRAITGASLADIMAKRNQKPEVRKAQREQAIRAAKEAKKAKQASKKTAMAAAKAPTKAAPKQKIVKPVKVSAPRVGGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JU34</accession>
    <name>A0A9L0JU34_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">GDP-fucose protein O-fucosyltransferase 2</fullName>
            <ecNumber evidence="4">2.4.1.221</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Peptide-O-fucosyltransferase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">POFUT2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X-X-S/T-C2 of thrombospondin type I repeats (TSRs) where C1 and C2 are the first and second cysteines of the repeat, respectively. O-fucosylates members of several protein families including the ADAMTS, the thrombospondin (TSP) and spondin families. Required for the proper secretion of ADAMTS family members such as ADAMTSL1 and ADAMTS13. The O-fucosylation of TSRs is also required for restricting epithelial to mesenchymal transition (EMT), maintaining the correct patterning of mesoderm and localization of the definite endoderm.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + GDP-beta-L-fucose = 3-O-(alpha-L-fucosyl)-L-seryl-[protein] + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63644"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:17914"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:189632"/>
            <dbReference type="EC" id="2.4.1.221"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:63645"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + GDP-beta-L-fucose = 3-O-(alpha-L-fucosyl)-L-threonyl-[protein] + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70491"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:17915"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57273"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:189631"/>
            <dbReference type="EC" id="2.4.1.221"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:70492"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the glycosyltransferase 68 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.221" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044606808.1">
        <property type="nucleotide sequence ID" value="XM_044750873.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JU34"/>
    <dbReference type="Ensembl" id="ENSEAST00005082181.1">
        <property type="protein sequence ID" value="ENSEASP00005052655.1"/>
        <property type="gene ID" value="ENSEASG00005007081.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106842979"/>
    <dbReference type="CTD" id="23275"/>
    <dbReference type="GeneTree" id="ENSGT00390000007989"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046922">
        <property type="term" value="F:peptide-O-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006004">
        <property type="term" value="P:fucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001707">
        <property type="term" value="P:mesoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903334">
        <property type="term" value="P:positive regulation of protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010717">
        <property type="term" value="P:regulation of epithelial to mesenchymal transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051046">
        <property type="term" value="P:regulation of secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd11298">
        <property type="entry name" value="O-FucT-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11340:FF:000002">
        <property type="entry name" value="GDP-fucose protein O-fucosyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11350:FF:000002">
        <property type="entry name" value="GDP-fucose protein O-fucosyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019378">
        <property type="entry name" value="GDP-Fuc_O-FucTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045130">
        <property type="entry name" value="OFUT2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13398">
        <property type="entry name" value="GDP-FUCOSE PROTEIN O-FUCOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13398:SF0">
        <property type="entry name" value="GDP-FUCOSE PROTEIN O-FUCOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10250">
        <property type="entry name" value="O-FucT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0294">Fucose metabolism</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040169604" description="GDP-fucose protein O-fucosyltransferase 2" evidence="20">
        <location>
            <begin position="22"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00026232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00033083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00057700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="429" mass="49413" checksum="03C86A95D753529C" modified="2023-09-13" version="1">MAALGVVCLLLAAACWRPASASGEEFWPGQSAADILSGAASRRRYLLYDVNPPEGFNLRRDVYIRVASLLKTLLKTEEWVLVLPPWGRLYHWQSPDILQVRIPWSDFFDLPSLNRNIPVIEYEQFIAESGGPFIDQVYVLQGYAEGWKEGSWEEKVDDRPCIDPLLYSQDKHEYYRGWFWGYEETRGLNVSCLSVQGSASVVAPVLLRNTSARSVMLDRAENLLHDHYGGKEYWDTRRSMVFAKHLRAVGDEFRSRYLNSTDAADRTPFEEDWTRMKVKLGSSLGGPYLGVHLRRKDFIWGHREDVPSLDGAVKKIRSLMKTYQLGKVFVATDAVRAEYEQLKQLLPEMVRFEPTWEELELYKDGGVAIIDQWICSHARFFIGTSVSTFSFRIHEEREILGLDPKTTYNRFCGDQEAACEQPTHWKIAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JVT1</accession>
    <name>A0A9L0JVT1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF4605 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">FAM241A</name>
    </gene>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Guo"/>
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    <accession>A0A9L0K260</accession>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <proteinExistence type="inferred from homology"/>
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        <location>
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    <feature type="chain" id="PRO_5040111296" evidence="5">
        <location>
            <begin position="35"/>
            <end position="286"/>
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            <begin position="40"/>
            <end position="145"/>
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    <feature type="domain" description="Cystatin LXN-type" evidence="6">
        <location>
            <begin position="165"/>
            <end position="268"/>
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        <source>
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            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS52033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005055730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="286" mass="31898" checksum="9438128F9BE8FDCA" modified="2023-09-13" version="1">MQPRRPPPPAPGPPRPAAPLLALPLLLLAVQGAAAPTGPEAAEWRPDTGPPHGLLRQAARAALHFLNFRAGSPSALRVLAAVQEGRARINAKKECTVDLVFTTERYNSEEGEERLGKCSARVFFRNQKPRPAVNVTCARLMEKTKRQQEDYLLYKQMKQLRNPLDGVSIPDSHGHIDPSLRPVWELAFLGSSYVMWEKTTRFLHYYMAQLSGVKQWETNDGAIDFDYTVLLHEFSTQEIIPCRIHLVWSPGKPLTVKYHCQELLAPEDASGTEEGSAVAPTELSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K4A2</accession>
    <name>A0A9L0K4A2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Integrin beta-1-binding protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Melusin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ITGB1BP2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">May play a role during maturation and/or organization of muscles cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with beta-1 integrin subunit. This interaction is regulated by divalent cations, and it occurs only in absence of calcium.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014693180.1">
        <property type="nucleotide sequence ID" value="XM_014837694.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K4A2"/>
    <dbReference type="SMR" id="A0A9L0K4A2"/>
    <dbReference type="Ensembl" id="ENSEAST00005054574.1">
        <property type="protein sequence ID" value="ENSEASP00005056170.1"/>
        <property type="gene ID" value="ENSEASG00005036980.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106828874"/>
    <dbReference type="KEGG" id="eai:106828874"/>
    <dbReference type="CTD" id="26548"/>
    <dbReference type="GeneTree" id="ENSGT00940000159429"/>
    <dbReference type="OrthoDB" id="10261079at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017124">
        <property type="term" value="F:SH3 domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06488">
        <property type="entry name" value="p23_melusin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1130.20:FF:000001">
        <property type="entry name" value="Cysteine and histidine-rich domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000027">
        <property type="entry name" value="integrin beta-1-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1130.20:FF:000004">
        <property type="entry name" value="integrin beta-1-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1130.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007051">
        <property type="entry name" value="CHORD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039790">
        <property type="entry name" value="CHRD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46983">
        <property type="entry name" value="CYSTEINE AND HISTIDINE-RICH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46983:SF2">
        <property type="entry name" value="INTEGRIN SUBUNIT BETA 1 BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04968">
        <property type="entry name" value="CHORD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51401">
        <property type="entry name" value="CHORD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0729">SH3-binding</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CHORD" evidence="11">
        <location>
            <begin position="5"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="CHORD" evidence="11">
        <location>
            <begin position="150"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="CS" evidence="10">
        <location>
            <begin position="216"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="316"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="321"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073516"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00083652"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005056170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="348" mass="38334" checksum="8CA72CAC7DD0B8FE" modified="2023-09-13" version="1">MSLLCHNKGCGQHFDPSTNLPDSCCHHPGVPIFHDALKGWSCCRKRTVDFSEFLNIKGCTVGPHCAEKLPEAPQPEGPATSNSLQEQKPLNMIPKSAETLRRERPKSELLPKLLPLNISQALEMALEQKELDQKPGAGLDSSLIRTGSSCQNPGCDAVYQGPESDATPCTYHPGAPRFHEGMKSWSCCGIQTLDFGAFLAQPGCRVGRHDWGKQLPASCRHDWHQTDSLVVVTVYGQIPLPAFNWVKASQTELHVHIVFDGNRVFQAQMKLWGVINVEQSSVSLMPSRVEISLVKADPGSWAQLEHPDALAEKAKAGVGLEMDEEESEDSDDDLSWTEEEEEEEAMGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K5D0</accession>
    <name>A0A9L0K5D0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Derlin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Functional component of endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins. May act by forming a channel that allows the retrotranslocation of misfolded proteins into the cytosol where they are ubiquitinated and degraded by the proteasome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the derlin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K5D0"/>
    <dbReference type="Ensembl" id="ENSEAST00005083591.1">
        <property type="protein sequence ID" value="ENSEASP00005060554.1"/>
        <property type="gene ID" value="ENSEASG00005025504.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063156"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007599">
        <property type="entry name" value="DER1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11009">
        <property type="entry name" value="DER1-LIKE PROTEIN, DERLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04511">
        <property type="entry name" value="DER1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040247476" description="Derlin" evidence="9">
        <location>
            <begin position="23"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="104"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0KKL6"/>
    <dbReference type="Ensembl" id="ENSEAST00005049806.1">
        <property type="protein sequence ID" value="ENSEASP00005062375.1"/>
        <property type="gene ID" value="ENSEASG00005028736.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000161684"/>
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        <property type="component" value="Chromosome 26"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
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        <property type="entry name" value="Znf_C2H2_sf"/>
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        <property type="entry name" value="ZINC FINGER PROTEIN 587B"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="107"/>
            <end position="134"/>
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    </feature>
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            <begin position="135"/>
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            <begin position="275"/>
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        <location>
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        <location>
            <begin position="334"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
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</entry>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="6" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Clathrin-coated vesicle</location>
        </subcellularLocation>
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    <dbReference type="Ensembl" id="ENSEAST00005000380.2">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:phosphatidylinositol phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="DENN domain containing 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11500:FF:000001">
        <property type="entry name" value="Putative DENN domain-containing protein 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR001194">
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    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
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    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
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    <dbReference type="InterPro" id="IPR040032">
        <property type="entry name" value="DENND1A/B/C"/>
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    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13196">
        <property type="entry name" value="DENN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13196:SF25">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03455">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00799">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="uDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="5">
        <location>
            <begin position="13"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="498"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="595"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="513"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="624"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="654"/>
            <end position="665"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="695"/>
            <end position="708"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="729"/>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="756"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="791"/>
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        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004132"/>
        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50211"/>
        </source>
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            <dbReference type="Ensembl" id="ENSEASP00005000299.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KS47</accession>
    <name>A0A8C4KS47_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106830365</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014695433.1">
        <property type="nucleotide sequence ID" value="XM_014839947.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KS47"/>
    <dbReference type="Ensembl" id="ENSEAST00005001512.2">
        <property type="protein sequence ID" value="ENSEASP00005001339.2"/>
        <property type="gene ID" value="ENSEASG00005001109.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106830365"/>
    <dbReference type="KEGG" id="eai:106830365"/>
    <dbReference type="GeneTree" id="ENSGT01150000286905"/>
    <dbReference type="OrthoDB" id="9444602at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071396">
        <property type="term" value="P:cellular response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15222">
        <property type="entry name" value="7tmA_OR51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000002">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF42">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="198"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="244"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="43"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001339.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="317" mass="35464" checksum="4C5F1FE612408FF1" modified="2023-09-13" version="2">MGFTNHSWFQPPTLFLTGIPGLEAVQIWISIPLCIMYLITFLGNCTILFVIKSTSSLHEPQYMFLSMLAGTDLDLSLSTLPTVLKVFLLNHREIEFHSCLTQMFFIHTFSSMESAILLAMAFDRFVAICNPLLYTVVLTPPRIIGMGIAAVVRGVVLMVPLPVLLQRLSFCKDVILSHCYCYHPDVMKLACGPVRVNIIYGLSLVLCSFGVDSMFFVISYILILKTVMSIASEDGQLKAFNTCASHIFTVCIFYVPLIVLALIHRFGTFTSPLLHVTMANLFLLLTPVLNPLVYSLKTKQIRSAVHKVFKSRGNLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KUZ5</accession>
    <name>A0A8C4KUZ5_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mitochondrial ribosomal protein S21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MRPS21</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS21 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014720898.1">
        <property type="nucleotide sequence ID" value="XM_014865412.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014720899.1">
        <property type="nucleotide sequence ID" value="XM_014865413.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070352960.1">
        <property type="nucleotide sequence ID" value="XM_070496859.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KUZ5"/>
    <dbReference type="SMR" id="A0A8C4KUZ5"/>
    <dbReference type="Ensembl" id="ENSEAST00005002183.2">
        <property type="protein sequence ID" value="ENSEASP00005001976.1"/>
        <property type="gene ID" value="ENSEASG00005001557.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106846553"/>
    <dbReference type="KEGG" id="eai:106846553"/>
    <dbReference type="CTD" id="54460"/>
    <dbReference type="GeneTree" id="ENSGT00500000045038"/>
    <dbReference type="OMA" id="MRHAQFL"/>
    <dbReference type="OrthoDB" id="2501249at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1150:FF:000002">
        <property type="entry name" value="28S ribosomal protein S21, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1150">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001911">
        <property type="entry name" value="Ribosomal_bS21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038380">
        <property type="entry name" value="Ribosomal_bS21_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00030">
        <property type="entry name" value="S21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21109">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21109:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS21M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01165">
        <property type="entry name" value="Ribosomal_S21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
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    <sequence length="87" mass="10676" checksum="D28696851F9150E7" modified="2022-01-19" version="1">MAKHLKFIARTVMVQEGNVEGAYRTLNRILTMDGLIEDIKRRRYYEKPCRRRQRESYETCRRIYNMEMARKINFLMRKNRADPWQGC</sequence>
</entry>
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        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
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        <name evidence="13" type="primary">LOC106828918</name>
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        <name type="scientific">Equus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
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                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Li"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Jin"/>
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                <person name="M."/>
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                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
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        <property type="entry name" value="OLFACTORY RECEPTOR 56B2-RELATED"/>
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        <property type="entry name" value="OLFACTORYR"/>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="32"/>
            <end position="55"/>
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</entry>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="Huang"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="Ji"/>
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                <person name="Jin"/>
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                <person name="Liu"/>
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                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="2">May be a bridge molecule between BCL2 and ARHGAP1/CDC42 in promoting cell death.</text>
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        <property type="term" value="P:negative regulation of cell population proliferation"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
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        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="190"/>
            <end position="351"/>
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    </feature>
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        <location>
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            <begin position="7"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="32"/>
            <end position="50"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="118"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00055513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00065286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00073394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005002680.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="356" mass="39698" checksum="FAF6C77CF0A203CB" modified="2023-09-13" version="2">MGTMQEAGEKTFDLGVRENAEAAGLAASPRLGELEPKEEWQDEEFPRLPPEEADPSEDPDDPKRDSQAGTPSTLALCGRRPMRKRLSAPELRLNLAKGPGDAGASATHSVPSSSDGSSDLEVDELETPSDSEQLDSGHEFEWEDELPQAEGLGASEAAERLGRGCVWDMAGEDGRHWRVFRTGQQEQRVDMTVIEPYKKVLSHGGYHGDGLNAVILFASCYLPRSSIPNYTYVMEHLFRYMVGTLELLVAENYLLVHLSGGTSRAQVPPLSWMRQCYLTLDRRLRKNLRALVVVHATWYVKAFLALLRPFISSKFTRKIRFLNSLEELAQLISLDQVHIPEAVRRLDWDLHGSGGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KYC4</accession>
    <name>A0A8C4KYC4_EQUAS</name>
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        <submittedName>
            <fullName evidence="7">PABPN1 like, cytoplasmic</fullName>
        </submittedName>
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    <gene>
        <name evidence="7" type="primary">PABPN1L</name>
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    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <location evidence="1">Cytoplasm</location>
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    <dbReference type="AlphaFoldDB" id="A0A8C4KYC4"/>
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        <property type="component" value="Chromosome 28"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:poly(A) binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:maternal-to-zygotic transition of gene expression"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:negative regulation of protein ubiquitination"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:negative regulation of SCF-dependent proteasomal ubiquitin-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0000288">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="RRM_II_PABPN1L"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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        <property type="entry name" value="RBD_domain_sf"/>
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    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF27">
        <property type="entry name" value="EMBRYONIC POLYADENYLATE-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="140"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="18"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="221"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000914.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="271" mass="29629" checksum="79A40B4B3582100D" modified="2023-09-13" version="2">MWPFLSRTLFPPPTEAWLQRASSDPEAQGWGAWSRTEKSSLGPGGEEREAKEADEEAGFLLSLLEREDLAECPVHDQELEAIKLKLWAMEQAQGPEQPRAQAQAGEEAGMGTTLAGQLLSPKTGCPSPGTPTEKVQADHRSIYVGNVDYGGTAEELEAYFNQCGEIRRVTILCDKFSGHPKGYAYIEFATESAAQTAVELDESIFRGRVIKVLPKRTNLPGISSTDRGGLRGHPGARGEALPHSSLQGGTRFRPRGRGRCLARCSPWYSPY</sequence>
</entry>
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            <fullName evidence="12">Olfactory receptor</fullName>
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    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="X."/>
                <person name="Zhao"/>
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                <person name="S."/>
                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Guo"/>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Yang"/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
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                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
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        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A8C4KYP1"/>
    <dbReference type="Ensembl" id="ENSEAST00005001253.2">
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        <property type="gene ID" value="ENSEASG00005000924.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286908"/>
    <dbReference type="OMA" id="TVFYVPM"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
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    <dbReference type="GO" id="GO:0005886">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0004930">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071396">
        <property type="term" value="P:cellular response to lipid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd15222">
        <property type="entry name" value="7tmA_OR51-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000002">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF6">
        <property type="entry name" value="OLFACTORY RECEPTOR 51T1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
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</entry>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
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            <taxon>Eutheria</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 12">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the oligosaccharyltransferase (OST) complex. OST exists in two different complex forms which contain common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either STT3A or STT3B as catalytic subunits, and form-specific accessory subunits. STT3A complex assembly occurs through the formation of 3 subcomplexes. Subcomplex 1 contains RPN1 and TMEM258, subcomplex 2 contains the STT3A-specific subunits STT3A, DC2/OSTC, and KCP2 as well as the core subunit OST4, and subcomplex 3 contains RPN2, DAD1, and OST48. The STT3A complex can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes. Interacts with DDI2. Interacts with TMEM35A/NACHO.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Endoplasmic reticulum membrane</location>
            <topology evidence="2 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="4 12">Belongs to the SWP1 family.</text>
    </comment>
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        <property type="gene ID" value="ENSEASG00005003220.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002635"/>
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        <property type="component" value="Chromosome 15"/>
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        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055375">
        <property type="entry name" value="Ribophorin_II_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055374">
        <property type="entry name" value="Ribophorin_II_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056790">
        <property type="entry name" value="Ribophorin_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055373">
        <property type="entry name" value="Ribophorin_II_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008814">
        <property type="entry name" value="Swp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640">
        <property type="entry name" value="RIBOPHORIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05817">
        <property type="entry name" value="Ribophorin_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23861">
        <property type="entry name" value="Ribophorin_II_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23860">
        <property type="entry name" value="Ribophorin_II_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25147">
        <property type="entry name" value="Ribophorin_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0732">Signal</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040528731" description="Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2" evidence="12">
        <location>
            <begin position="23"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="511"/>
            <end position="531"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="543"/>
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    </feature>
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        <location>
            <begin position="576"/>
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    </feature>
    <feature type="domain" description="Ribophorin II N-terminal" evidence="13">
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            <begin position="29"/>
            <end position="263"/>
        </location>
    </feature>
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            <begin position="271"/>
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    </feature>
    <feature type="domain" description="Ribophorin II third" evidence="14">
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            <begin position="377"/>
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    </feature>
    <feature type="domain" description="Ribophorin II C-terminal" evidence="16">
        <location>
            <begin position="501"/>
            <end position="599"/>
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    </feature>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00017612"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366029"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF05817"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF23860"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF23861"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF25147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005004250.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="602" mass="65709" checksum="E9ED4DFE2E333073" modified="2023-09-13" version="2">MAPPGSSTVFLSALTIIASAQALTPTHYLTKHDVERLKASLDRPFTSLESAFYSIVGLSSLGAQVPDVKKACAFVKANLDPSNVDSLFYAAQSSQALSGCEISISNETKDLLLAAVSEDSSVTQIYHAVAALSGFGLPLASQEALGALTTRLSKEETVLATIQALQTASHLSQQADLRTVVEEIEDLVARLDELGGVYLQFEEGLETTALFVAATYKLMDHVGTEPSIKEDQVIQLMNAIFSKKNFESLSEAFSVASAAAALSQNRYHVPVVVVPEGTPSDTHEQAILRLQVTNVLSQPLTQATVKLEHAKSAASRATVLQKTSFTPVGDVFELNFMSVKFSSGYYDFSVKVEGDSRYIANSVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFVADSHQNFALFFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAEPDSKNVYKFELDTSERKIEFDSASGTYTLYLIIGDATLKNPILWNVHLFREPEKRPPTVVSNTFTALILSPLLLLFALWIRIGANVSNFTFAPSTIIFHLGHAAMLGLMYVYWTQLNMFQTLKYLAILGSVTFLAGNRMLAQQAIKRTAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L2I9</accession>
    <name>A0A8C4L2I9_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">PBX/knotted 1 homeobox 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PKNOX2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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                <person name="Zhang"/>
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                <person name="Liu"/>
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        <property type="entry name" value="neurobeachin isoform X1"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
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    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
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    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="entry name" value="WD40_repeat_CS"/>
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    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
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        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
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    <dbReference type="PANTHER" id="PTHR13743:SF86">
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    <dbReference type="Pfam" id="PF02138">
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        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="5 10" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="13">
        <location>
            <begin position="3007"/>
            <end position="3113"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="12">
        <location>
            <begin position="3118"/>
            <end position="3420"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="3610"/>
            <end position="3644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="918"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1182"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1182"/>
            <end position="1191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00064710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005004289.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="3799" mass="428812" checksum="95BC5F28CDE7C274" modified="2023-09-13" version="2">MSTDSNSLAREFLTDVNRLCNAVVQRVEAREEEEEETHMATLGQYLVHGRGFLLLTKLNSIIDQALTCREELLTLLLSLLPLVWKIPVQEEKATDFHLPFSADIILTKEKISSSQRSAQEKLYLEGSAPSGQVSAKVNIFRKSRRQRKITHRYSIRDARKTQLSTSDSEANSDDKGIAVNKHKRSHLLQHFVTHSPKEDHLTAKLDHLSTKEEHTPPDTMALGNSREIIPRKESNSDTLSEPAALSIISHMNNSPFDLCHVLLSLLEKVCKFDITLNRNSALAASVVPTLTEFLAGFGDCCNLSDNLDSRMVSTGWTEEPVALIQRMLFRTVLHLMSVDISTAEMMPESLRKNLTELLRAALKIRTYLEKQPDAFAPRQKKTLQEVQEGFVFSKYRHRALLLPELLKGVLQIMICCLQSAASNPFFFSQAMDLVQEFIQHHGFNLFETTVLQMEWLVLRDGVPTEASEHLKALISSVIKIMSTVKKVKSEQLHHSVCTRKRHRRCEYSHFMHHHRDLSGLPVSTFKNQVSKNPFEETADGDVYYPERCCCIAVCAHQCLRLLLQASLKGTCVQILSGVHNIGICCCMDPKSVIIPLLHAFKLPALKNFQQHILNILNKLILDQLGGAEITQKIKKAACNICTIDSDQLAKLEETLQGNSWDAEPSSSLSSPSYRFQGILPSSGCEALLWKWDALETYQNFVFEQDILHSLQIANHICSLIQKGNVFVQWKLYNYVFNPVLQRGVELAHHCQHLSNTSAQTHVCSHHNQCLPQEVLQIYLKTLPALLKSRVIRDLFLSCNGVNQIIELNYLDDIRSHSLKAFETLIISLGEQQKDSSIPGIDGIDVEQKELSSLNAGTSFHNQQAYSDSPQSLSKFYAGLKETCPKKQKTLNQNVHINMINLFLCVAFLCVSKEAESDRESANESEDTSGYDSTASEPLHHMLPSLSPESLVLPSPEQMHQAADIWLMCRWIYMLSPVFQKQFYRLGGFQVCHRLIFMIIQKLFRSREEEQGKKEGDININEIQDLNRTSESEVTMKEDLLSLTVKSDPTPSEQDSLKKSAESLHKLESQHISSVNVEQISTTKAASEEAKIFTSKESEPSLQSIRLLEALLAICLHSTRASQQKMELELPDQNLSVENILFEMRDHLSQSKVIETELAKPLFDALLRVALGNHSADFEHNDAMTEKSHQSEEELSSQPGDFSEEAEDSQCCSFKLLVEEEGYEADSESNPEDSEIRDDEVDLNPEAEGLSASSSPNDLLENPSQGEMIYPEICMLQLNLLSTSKAKLDVLAHVFESFLKIIRQKEKNIFLLMQQGTVKNLLGGFLSILTQADSDFQVCQRVLVDLLVSLMSSRTCSEELTLLLRIFLEKSPCTEILLLGLLKIVESDITMSPLQYLTFPLLHTPSLTNGVSSQKCPRILNSKALGLLRRARVSRSKKEADSEGFPQQLLSSWHIAPVHLPLLGQNCWPHLSEGFSVSLWFNVECIHEAEGTTERGKKIKKRNKSLILQDSSFDGTENNRPEGAEHINHGERLIEEGCIHMISLGSKALMIQVWADPHNGTFIFRVCMDSNDDMKAVLLAQAESQKNIFLPSKWQHLVLTYLEQPQGKKSIHGKISIWVSGQRKPDVTLDFMLPRKTNLSSDSNKTFCMIGHCLSSQEDILQLAGKWDLGNLLLFNGAKIGSQEAFYLYACGPNHTSIMPCKYGKPVNDYSKYINKEILQCEQIRELFMTKKDVDIGLLIESLSVVYTTYCPAQYTIYEPVIRLKGQMKTQLSQRPFSSKEVQSILLEPHHIKSLRPTERKTIQSILHEIGGTGVFVFPSFARVVELSSCEETQALALRVILSLIKYNQQRMCELENCNGLSVIHQVLIKQKCIVGFHILKILLEGCCGEDIIHINENGEFTLDVESNAIIQDVKLLEELLLDWKIWNKAEQGVWETLLAALEVLIRADHHQQMFNIKQLLKAQVVHHFLLTCQVVQEHKEGQLTSLPREVCKSFVKIIAEVLGSPPDLELLTIIFNFLLAVHPPTNTYVCHNPTNFYFSLHIDGKIFQEKVQSIMYLRHSSSGGKSVTSPGFMVISPSGFTASPPEGNNSSNIIPRRMAVNMLRSRSLPAFPTSSPLMQPRKLTGSLGCSIDKLQNIADNYVTSRPEKWNPLGSSETLKTGKEDAFLSSCESARAVCEMEAVLPAQASVNGVPKGSLGFPVVKELGAEPRSDDDSPGDESCPRRPDYLKGLASFQGSHSTIASLGLAFPSQNGAAAVGRWPSLVDRNTDDWENLAFSLRYEPNYNRTTNAHSVTEDCLVPICCGLYELLSGVLLILPDIMLEDVMDKLIQADTLLVLVNHPSPAIQQGVIKLLDAYFTRASKEQKDKFLKNRGFSLLANQLYLHRGTQELLECFIEMFFGRRIGLDEEFDLEDVKNLGLFQKWSVIPILGLIETSLCDNVLLHSALLLLLQILNSCSKVADMLLDNGLLYVLCNTVAALNGLEKNVPLNEYRLLACDIQRLFIAVTIHACSSSGSQYFRVIEDLIVLLGYLQNSKNKRTQNMAVALQFRVLQAAMEFIRTTANHDSENLTNSFPLPSAPHHAIFQKRKSIAGPRKFPLAQTDSLLMKMRSVASDELNMMMQRRMSQENPVQATETELAQRLQRLTILAVNRIIYQEFNPDIIDILSTPESATQSKNSVSQTEISEENIYHDQPSVFNPFQKEIFTYLVEGFKVSVGSSKTSGSKQQWTKILWSCKETFRMQLGRLLVHILSPAHPSQERKQIFEIVREPNHQEILRDCLSSSLQHGPKLVLYLSELIHNHHDELTEEELDAAQLLMNALKSCGHKCSPPSASAKSDLVKMIKEEQKKYETEEGVNKTTWQKRVNHNQQSLFQRLDSKSKDISKIAADITQAVSLSQGIERKKVIQHIRGMYKVDLSASRHWQELIQQLTHDRAVWYDPTYYPTSWQLDPTEGPNRERRRLQRCYLTIPNKYLLRDRQKSGDVVKPPLSYLFEDKTHSSFSSTVKDKAASESIRVNRRCISVAPSRETAGELLLGKCGMYFVEDNASDTVESSNLQGELEPASFSWTYEEIKEVHKRWWQLRDNAVEIFLTNGRTLLLAFDNTKVRDDVYHSILTNNLPNLLEYGNITALTNLWYTGQITNFEYLTHLNKHAGRSFNDLMQYPVFPFILADYVSETLDLSDPSVYRNLSKPIAVQYKEKEDRYVDTYKYLEEEYRKGAREDDPMPPVQPYHYGSHYSNSGTVLHFLVRMPPFTKMFLAYQDQSFDIPDRTFHSTNTTWRLSSFESMTDVKELIPEFFYLPEFLVNREGFDFGVRQNGERVNHVNLPPWARNDPRLFILIHRQALESDYVSQNICQWIDLVFGYKQKGKASVQAINVFHPATYFGMDVSAVEDPVQRRALETMIKTYGQTPRQLFQSAHASRPGSKLNIEGELPAAVGLLVQFAFRETREQVKEVTYPSPLSWIKGLKWGEYVGSPSAPVPVVCFSQPHGERFGSLQALPTRAICGLSRNFCLLMTYSKEQGVRSMNSTDIQWSAILSWGYADNVLRLKSKQSEPPVNFIQSSPQYQVTSCAWVPDSCQLFTGSKCGVITAYANRLTSSTPSEIEMESQIHLYGHTEEITSLFVCKPYSIMISVSRDGTCIIWDLNRLCYVQSLAGHKSPVTAVSASETTGDIATVCDSAGGGSDLRLWTVNGDLVGHVHCREIICSVAFSNQPEGVSINVIAGGLENGVVRLWSTWDLKPVREITFPKSNKPIVSLTFSCDGHHLYTANSDGTVIAWCRKDQQRLKQPMFYSFLSSYAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L822</accession>
    <name>A0A8C4L822_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 18">FACT complex subunit SSRP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">SSRP1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with MYOG (via C-terminal region). Component of the FACT complex, a stable heterodimer of SSRP1 and SUPT16H. Also a component of a CK2-SPT16-SSRP1 complex which forms following UV irradiation, composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B. Binds to histone H3-H4 tetramers, but not to intact nucleosomes. Identified in a centromere complex containing histones H2A, H2B and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1. Interacts with isoform gamma of TP63. Interacts with FYTTD1/UIF. Interacts with SRF. Interacts with NEK9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the SSRP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L822"/>
    <dbReference type="Ensembl" id="ENSEAST00005005680.2">
        <property type="protein sequence ID" value="ENSEASP00005005186.2"/>
        <property type="gene ID" value="ENSEASG00005003883.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157117"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902275">
        <property type="term" value="P:regulation of chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd21994">
        <property type="entry name" value="HMG-box_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13230">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13231">
        <property type="entry name" value="PH2_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.30.10:FF:000032">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.150:FF:000001">
        <property type="entry name" value="Fact complex subunit ssrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.220:FF:000001">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000098">
        <property type="entry name" value="Fact complex subunit ssrp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000119">
        <property type="entry name" value="FACT complex subunit SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.220">
        <property type="entry name" value="Structure-specific recognition protein (SSRP1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013719">
        <property type="entry name" value="RTT106/SPT16-like_middle_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050454">
        <property type="entry name" value="RTT106/SSRP1_HistChap/FACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048993">
        <property type="entry name" value="SSRP1-like_PH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000969">
        <property type="entry name" value="SSRP1/POB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035417">
        <property type="entry name" value="SSRP1/POB3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048985">
        <property type="entry name" value="SSRP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024954">
        <property type="entry name" value="SSRP1_DD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038167">
        <property type="entry name" value="SSRP1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45849:SF1">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SSRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21103">
        <property type="entry name" value="PH1_SSRP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17292">
        <property type="entry name" value="POB3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08512">
        <property type="entry name" value="Rttp106-like_middle"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03531">
        <property type="entry name" value="SSrecog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21092">
        <property type="entry name" value="SSRP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00887">
        <property type="entry name" value="SSRCOGNITION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01287">
        <property type="entry name" value="Rtt106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 18" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8 18" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14 18" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 18" id="KW-0235">DNA replication</keyword>
    <keyword evidence="12 17" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="15 17 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 18" id="KW-0804">Transcription</keyword>
    <keyword evidence="11 18" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="HMG box" evidence="20">
        <location>
            <begin position="550"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="17">
        <location>
            <begin position="550"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="461"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="473"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="500"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="518"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="537"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="580"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="628"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="646"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="699"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00065079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU364013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005186.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="712" mass="81557" checksum="115C7D3210FBE538" modified="2023-09-13" version="2">MAETLEFNDIYQEVKGSMNDGRLRLSRQGIIFKNSKTGKVDNIQAGELTEGIWRRVALGHGLKLLTKNGHVYKYDGFRESEFEKLSDFFKTHYRLELMEKDLCVKGWNWGTVKFGGQLLSFDIGDQPVFEIPLSNVSQCTTGKNEVTLEFHQNDDAEVSLMEVRFYVPPTQEDGVDPVEAFAQNVLSKADVIQATGDAICIFRELQCLTPRGRYDIRIYPTFLHLHGKTFDYKIPYTTVLRLFLLPHKDQRQMFFVISLDPPIKQGQTRYHFLILLFSKDEDISLTLNMNEEEVEKRFEGRLTKNMSGSLYEMVSRVMKALVNRKITVPGNFQGHSGAQCITCSYKASSGLLYPLERGFIYVHKPPVHIRFDEISFVNFARGTTTTRSFDFEIETKQGTQYTFSSIEREEYGKLFDFVNAKKLNIKNRGLKEKKEGMNPNYDEYADSDEDQHDAYLERMKEEGKIREENANDSSDDSGEETDESFNPGEEEEDVAEEFDSNASASSSSNEGDSDRDEKKRKQLKKAKMAKDRKSRKKPMEVKKGKDPNAPKRPMSAYMLWLNASREKIKSDHPGISITDLSKKAGEIWKGMSKEKKEEWDRKAEDARREYEKAMKEYEGGRGESSKRDKSKKKKKVKVKMEKKSTPSRGSSSKSSPRQLSESFKSKEFVSSDESSSGENKSKKKRRRSEDSEEEELASTPPSSEDSASGSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LC51</accession>
    <name>A0A8C4LC51_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Microfibril associated protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MFAP1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in pre-mRNA splicing as a component of the spliceosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the spliceosome B complex. Interacts with PRPF38A (via N-terminal interaction domain).</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MFAP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014702577.1">
        <property type="nucleotide sequence ID" value="XM_014847091.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LC51"/>
    <dbReference type="SMR" id="A0A8C4LC51"/>
    <dbReference type="Ensembl" id="ENSEAST00005007149.2">
        <property type="protein sequence ID" value="ENSEASP00005006535.1"/>
        <property type="gene ID" value="ENSEASG00005004835.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835011"/>
    <dbReference type="KEGG" id="eai:106835011"/>
    <dbReference type="CTD" id="4236"/>
    <dbReference type="GeneTree" id="ENSGT00690000102225"/>
    <dbReference type="OMA" id="FHNERAG"/>
    <dbReference type="OrthoDB" id="1111734at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001527">
        <property type="term" value="C:microfibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071005">
        <property type="term" value="C:U2-type precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033194">
        <property type="entry name" value="MFAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009730">
        <property type="entry name" value="MFAP1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15327">
        <property type="entry name" value="MICROFIBRIL-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06991">
        <property type="entry name" value="MFAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Micro-fibrillar-associated protein 1 C-terminal" evidence="6">
        <location>
            <begin position="191"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="39"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="210"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="280"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="71"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="131"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF06991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="439" mass="51958" checksum="0EF6F4F091C05A88" modified="2022-01-19" version="1">MSVPSALMKQPPIQSTAGAVPVRNEKGEISMEKVKVKRYVSGKRPDYAPMESSDEEDEEFQFIKKAKEQEAEPEEQEEDSSSDPRLRRLQNRISEDVEERLARHRKIVEPEVVGESDSEVEGDAWRMEREDSSEEEEEEIDDEEIERRRGMMRQRAQERKNEEMEVMEVEDEGRSGEESESESEYEEYTDSEDEMEPRLKPVFIRKKDRVTVQEREAEALKQKELEQEAKRMAEERRKYTLKIVEEETKKELEENKRSLAALDALNTDDENDEEEYEAWKVRELKRIKRDREDREALEKEKAEIERMRNLTEEERRAELRANGKVITNKAVKGKYKFLQKYYHRGAFFMDEDEEVYKRDFSAPTLEDHFNKTILPKVMQVKNFGRSGRTKYTHLVDQDTTSFDSAWGQESAQNTKFFKQKAAGVRDVFERPSAKKRKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LEM0</accession>
    <name>A0A8C4LEM0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LIM and senescent cell antigen-like-containing domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LIMS2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="9">Cell junction</location>
            <location evidence="9">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_070363462.1">
        <property type="nucleotide sequence ID" value="XM_070507361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LEM0"/>
    <dbReference type="Ensembl" id="ENSEAST00005010926.2">
        <property type="protein sequence ID" value="ENSEASP00005010055.2"/>
        <property type="gene ID" value="ENSEASG00005007130.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106828960"/>
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    <dbReference type="GeneTree" id="ENSGT00940000153518"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
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    <dbReference type="GO" id="GO:0005911">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="term" value="P:integrin-mediated signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900026">
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09331">
        <property type="entry name" value="LIM1_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09332">
        <property type="entry name" value="LIM2_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09333">
        <property type="entry name" value="LIM3_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09334">
        <property type="entry name" value="LIM4_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09335">
        <property type="entry name" value="LIM5_PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000011">
        <property type="entry name" value="Lim and senescent cell antigen-like-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000017">
        <property type="entry name" value="Lim and senescent cell antigen-like-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000019">
        <property type="entry name" value="Lim and senescent cell antigen-like-containing"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000021">
        <property type="entry name" value="Lim and senescent cell antigen-like-containing"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000029">
        <property type="entry name" value="LIM and senescent cell antigen-like-containing domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047944">
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    </dbReference>
    <dbReference type="InterPro" id="IPR017351">
        <property type="entry name" value="PINCH-1-4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047946">
        <property type="entry name" value="PINCH-1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24210:SF10">
        <property type="entry name" value="LIM AND SENESCENT CELL ANTIGEN-LIKE-CONTAINING DOMAIN PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24210">
        <property type="entry name" value="LIM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038003">
        <property type="entry name" value="PINCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0965">Cell junction</keyword>
    <keyword evidence="2 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0440">LIM domain</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="38"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="101"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="221"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="10">
        <location>
            <begin position="281"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005010055.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="366" mass="41440" checksum="349C70D266D6EA44" modified="2023-09-13" version="2">MAARLGALAASGLYRRRQHRQSPPPAAPGNMSNALANAVCQRCQARFAPAERIVNSNGELYHEHCFVCAQCFRPFPEGLFYEFEGRKYCEHDFQMLFAPCCGSCGEFIIGRVIKAMNNSWHPGCFRCELCDVELADLGFVKNAGRHLCRPCHNREKAKGLGKYICQRCHLVIDEQPLMFRNDAYHPDHFSCTHCGKELTAEARELKGELYCLPCHDKMGVPICGACRRPIEGRVVNALGKQWHVEHFVCAKCEKPFLGHRHYEKKGLAYCETHYNQLFGDVCYNCGRVIEGDVVSALNKAWCVNCFSCSTCNGKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELAARKAHPKSVGLNSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LG40</accession>
    <name>A0A8C4LG40_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NRAS</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_044605475.1">
        <property type="nucleotide sequence ID" value="XM_044749540.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LG40"/>
    <dbReference type="SMR" id="A0A8C4LG40"/>
    <dbReference type="Ensembl" id="ENSEAST00005009533.2">
        <property type="protein sequence ID" value="ENSEASP00005008751.1"/>
        <property type="gene ID" value="ENSEASG00005006279.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835678"/>
    <dbReference type="KEGG" id="eai:106835678"/>
    <dbReference type="CTD" id="4893"/>
    <dbReference type="GeneTree" id="ENSGT00940000158947"/>
    <dbReference type="OMA" id="RAVDIWG"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001938">
        <property type="term" value="P:positive regulation of endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd04138">
        <property type="entry name" value="H_N_K_Ras_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000096">
        <property type="entry name" value="KRAS proto-oncogene, GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0636">Prenylation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="189" mass="21229" checksum="6898D3F6815B1EC7" modified="2022-01-19" version="1">MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNSKSFADINLYREQIKRVKDSDDVPMVLVGNKCDLPTRTVDTKQAHELAKSYGIPFIETSAKTRQGVEDAFYTLVREIRQYRMKKLNSSDDGTQGCMGLPCVVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LH97</accession>
    <name>A0A8C4LH97_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PAX3 and PAX7 binding protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">PAXBP1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GCF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LH97"/>
    <dbReference type="Ensembl" id="ENSEAST00005009432.2">
        <property type="protein sequence ID" value="ENSEASP00005008654.2"/>
        <property type="gene ID" value="ENSEASG00005006214.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000455"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990226">
        <property type="term" value="F:histone methyltransferase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000288">
        <property type="term" value="P:positive regulation of myoblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014842">
        <property type="term" value="P:regulation of skeletal muscle satellite cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012890">
        <property type="entry name" value="GCFC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022783">
        <property type="entry name" value="GCFC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12214">
        <property type="entry name" value="GC-RICH SEQUENCE DNA-BINDING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12214:SF2">
        <property type="entry name" value="PAX3- AND PAX7-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07842">
        <property type="entry name" value="GCFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GCF C-terminal" evidence="5">
        <location>
            <begin position="651"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="198"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="283"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="585"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
        <location>
            <begin position="128"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="216"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="597"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008654.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="972" mass="110032" checksum="3FFBB630405E7E22" modified="2023-09-13" version="2">MRARGRGGALRAVAGGKTGPGWSTGGKRACAVASSAQPGPPCGSECETAVRAAGMFRKARRVNVRKRNDSEEEERERDEEQEPPPLLPPPGTGEEPGPGGGGDRAPGGESLLGPGLPPPSALTPGPGAEAGGCFPGGAEPGNGLKPRKRPRENKEVPRASLLSFQDEEEENEEVFKVKKSSYSKKIVKLLKKEYKEDLEKSKIKTELNSSADNEPSLDKTGHVKDTSQEDGVIISEHGEDEMDMESEKEEEKPKAGGAFSNALSSLNVLRPGEIPDAAFIHAARKKRQMARELGDFTPHDSEPGKGRLVREDENDASDDEDDDEKRRIVFSVKEKSQRQKIAEEIGIEGSDDDALVTGEQDEELSRWEQEQIRKGINIPQVQASQPTEVNMYYQNTYQTMPYGSSYGIPYSYTAYGSSDAKSQKTDNTVPFKTPSNEMTPVTIDLVKKQLKDRLDSMKELHKTNRQQHEKHLQSRVDSTRAIERLEGSSGGIGERYKFLQEMRGYVQDLLECFSEKVPLINELESAIHQLYKQRASRLVQRRQDDIKDESSEFSSHSNKALMAPNLDSFGRDRALYQEHAKRRIAEREARRTRRRQAREQTGKMADHLEGLSSDDEETSTDITNFNLEKDRISKESSKVFEDVLESFYSIDCIKSQFEAWRSKYYMSYKDAYIGLCLPKLFNPLIRLQLLTWTPLEAKCRDFETMLWFESLLFYGCEEREQEKDDVDVALLPTIVEKVILPKLTVIAENMWDPFSTTQTSRMVGITLKLINGYPSVVNAENKNTQVYLKALLLRMRRTLDDDVFMPLYPKNVLENKNSGPYLFFQRQFWSSVKLLGNFLQWYGIFSNKTLQELSIDGLLNRYILMAFQNSEYGDDSIKKAQNVINCFPKQWFMNLKGERTISQLENFCRYLVHLADTIYRNSIGCSDVEKRNARENIKQIVKLLASVRALDHAMSVASDHNVKEFKSLIEGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LHD7</accession>
    <name>A0A8C4LHD7_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">E74 like ETS transcription factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ELF2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ETS family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014706582.1">
        <property type="nucleotide sequence ID" value="XM_014851096.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070360855.1">
        <property type="nucleotide sequence ID" value="XM_070504754.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070360856.1">
        <property type="nucleotide sequence ID" value="XM_070504755.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070360857.1">
        <property type="nucleotide sequence ID" value="XM_070504756.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070360858.1">
        <property type="nucleotide sequence ID" value="XM_070504757.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070360859.1">
        <property type="nucleotide sequence ID" value="XM_070504758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LHD7"/>
    <dbReference type="Ensembl" id="ENSEAST00005012155.2">
        <property type="protein sequence ID" value="ENSEASP00005011178.2"/>
        <property type="gene ID" value="ENSEASG00005007869.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837397"/>
    <dbReference type="KEGG" id="eai:106837397"/>
    <dbReference type="CTD" id="1998"/>
    <dbReference type="GeneTree" id="ENSGT00940000154953"/>
    <dbReference type="OrthoDB" id="8196042at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000066">
        <property type="entry name" value="ETS-related transcription factor Elf-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000418">
        <property type="entry name" value="Ets_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046328">
        <property type="entry name" value="ETS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022084">
        <property type="entry name" value="TF_Elf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11849:SF10">
        <property type="entry name" value="ETS-RELATED TRANSCRIPTION FACTOR ELF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12310">
        <property type="entry name" value="Elf-1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00178">
        <property type="entry name" value="Ets"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00454">
        <property type="entry name" value="ETSDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00413">
        <property type="entry name" value="ETS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00345">
        <property type="entry name" value="ETS_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00346">
        <property type="entry name" value="ETS_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50061">
        <property type="entry name" value="ETS_DOMAIN_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="5 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="ETS" evidence="11">
        <location>
            <begin position="208"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="146"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="171"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011178.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="594" mass="63988" checksum="463049122CAB645B" modified="2023-09-13" version="2">MASAVVDSGGSILELSSNGVENEEESEKVSEYPAVIVEPVPSARLEQGYAAQVLVYDDETYMMQDVAEEQEVETENVETVEASVHSSNAHCTDKTIEAAEALLHMESPTCLRDSRSPVEVFVPPCVSTPEFIHAAMRPDVITETVVEVSTEESEPMDTSPIPTSPDSHEPMKKKKVGRKPKTQQSPISNGSPELGIKKKPREGKGNTTYLWEFLLDLLQDKNTCPRYIKWTQREKGIFKLVDSKAVSKLWGKHKNKPDMNYETMGRALRYYYQRGILAKVEGQRLVYQFKDMPKNIVVIDDDKSETCNEDLAAAADEKSLERVSLSTESLLKAATSVRGGKNSSPLNCSRAEKGVARVVNITSPGHDASSRSPTTTASVSATAAPRTVRVAMQVPVVMTSLGQKISTVAVQSVNAGAPLITSTSPTTATSPKVVIQTIPTVMPASTENGDKITMQPAKIITIPATQLAQCQLQTKSNLTGSGSINIVGTPLAVRALTPVSIAHGTPVMRLSVPTQQASGQTPPRVISAVIKGPEVKSEAVAKKQEHDVKTLQLVQEEKPADGNKTVTHVVVVSAPSAIALPVTMKTEGLVTCEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LJN3</accession>
    <name>A0A8C4LJN3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 248</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZNF248</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LJN3"/>
    <dbReference type="Ensembl" id="ENSEAST00005010525.2">
        <property type="protein sequence ID" value="ENSEASP00005009692.2"/>
        <property type="gene ID" value="ENSEASG00005006887.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161962"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000355">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 20 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000914">
        <property type="entry name" value="Zinc finger protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000060">
        <property type="entry name" value="zinc finger protein 436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001157">
        <property type="entry name" value="Zinc finger protein 793"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050826">
        <property type="entry name" value="Krueppel_C2H2_ZnFinger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24377">
        <property type="entry name" value="IP01015P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="10" id="KW-0804">Transcription</keyword>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LLS0"/>
    <dbReference type="Ensembl" id="ENSEAST00005012954.2">
        <property type="protein sequence ID" value="ENSEASP00005011920.2"/>
        <property type="gene ID" value="ENSEASG00005008351.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000287003"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011920.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="93" mass="10526" checksum="39417F53DD262CEE" modified="2023-09-13" version="2">MLERVWREGNPHTLLVGMQTAAATMENSMEIPQNTKNRTTYDPAIPLLGIYPNNLKSTIQSHICTPMLIAALFTIAKTWKQPKCPSTDDWIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LR66</accession>
    <name>A0A8C4LR66_EQUAS</name>
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        <recommendedName>
            <fullName evidence="21">Cadherin EGF LAG seven-pass G-type receptor 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Multiple epidermal growth factor-like domains protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="36" type="primary">CELSR3</name>
    </gene>
    <organism evidence="36 37">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="36 37" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="36" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="36" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Receptor that may have an important role in cell/cell signaling during nervous system formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005015989.2">
        <property type="protein sequence ID" value="ENSEASP00005014699.2"/>
        <property type="gene ID" value="ENSEASG00005010210.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160077"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000011">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000013">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1240.10:FF:000021">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000108">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000089">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000286">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000311">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000074">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000084">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000010">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000023">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000029">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000038">
        <property type="entry name" value="Cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.60.40.60:FF:000040">
        <property type="entry name" value="cadherin EGF LAG seven-pass G-type receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000113">
        <property type="entry name" value="Cadherin, EGF LAG seven-pass G-type receptor 3"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000044">
        <property type="entry name" value="Cadherin, EGF LAG seven-pass G-type receptor 3"/>
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    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1240.10">
        <property type="entry name" value="GPCR, family 2, extracellular hormone receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032471">
        <property type="entry name" value="AGRL2-4_GAIN_subdom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056286">
        <property type="entry name" value="Cadherin_CELSR1-3_9th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036445">
        <property type="entry name" value="GPCR_2_extracell_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001879">
        <property type="entry name" value="GPCR_2_extracellular_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026:SF38">
        <property type="entry name" value="CADHERIN EGF LAG SEVEN-PASS G-TYPE RECEPTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24026">
        <property type="entry name" value="FAT ATYPICAL CADHERIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23592">
        <property type="entry name" value="Cadherin_CELSR2_9th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16489">
        <property type="entry name" value="GAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02793">
        <property type="entry name" value="HRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00008">
        <property type="entry name" value="HormR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50227">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 23" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="15 24" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 24" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="19" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="20 25" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="14 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-0675">Receptor</keyword>
    <keyword evidence="37" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 28" id="KW-0732">Signal</keyword>
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    <keyword evidence="8 27" id="KW-0812">Transmembrane</keyword>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000313" key="37">
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            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="3317" mass="359155" checksum="EABC48EEC094F984" modified="2023-09-13" version="2">MARQPLWWSLRGPTTPLLLLLLLSLFPLSREELEDGGGQGWDPGIAAATGRGAQIGGGALALCPETPEIQEDGEPGLGIREPVFVGLRGGRQSTQSGRGPPEQPDPGLRAEYGVQALGSHGRETGQGPGSLLCWRPEISSCGKTGPLRRDSLSLEALSPGVPGPEDSSPFPSDLLVPPRGSKSVSSQRNAGRSALQKVGTIRCCGELWASGRRSQGEKRATSREERIVPRTDCPPGAAGSDPELVSAPRTARTAPASSSAPRKSRTAPEPAPERMRSRGLFRRRFLPQRPGPRPPGVPAEPGAWKTLPASRVRPRRAANRHPQFPQYNYQALVPENEAAGTVVLRVVAQDPDTGEAGRLVYSLAALMNSRSLELFSIDPQSGLIRTEAALDRESMERHYLRVTAQDHGSPRLSATTMVAVTVADRNDHAPVFEQAQYRETLRENVEEGYPILQLRATDGDAPPNANLRYRFVGSPAARAAAAAAFEIDPRSGLISTSGRVDREHMESYELVVEASDQGQEPGPRSATVRVHITVLDENDNAPQFSEKRYVAQVREDVRPHTVVLRVTATDRDKDANGLVHYNIISGNSRGHFAIDSLTGEIQVVAPLDFEAEREYALRIRAQDAGRPPLSNNTGLASIQVVDINDHTPIFVSTPFQVSVLENAPLGHSVIHIQAVDADHGENARLEYSLTGVAPDTPFVINSATGWVSVSGPLDRESVEHYFFGVEARDHGSPPLSASASVTVTVLDVNDNRPEFTMKEYHLRLNEDAAVGTSVVSVTAVDRDANSAISYQITGGNTRNRFAISTQGGVGLVTLALPLDYKQERYFKLVLTASDRALHDHCYVHINITDANTHRPVFQSAHYSVSVNEDWPVGSTVVVISASDDDVGENARITYLLEDNLPQFRIDADSGAITLQAPLDYEDQVTYTLAITARDNGIPQKADTTYVEVMVNDVNDNAPQFVASHYTGLVSEDAPPFTSVLQISATDRDAHANGRVQYTFQNGEDGDGDFTIEPTSGIVRTVRRLDREAVPVYELTAYAVDRGVPPLRTPVSIQVTVQDVNDNAPVFPAEEFEVRVKENSIVGSVVAQITAVDPDEGPNAHIMYQIVEGNIPELFQMDIFSGELTALIDLDYEARQEYVIVVQATSAPLVSRATVHVCLVDQNDNSPVLNNFQILFNNYVSNRSDTFPSGIIGRIPAYDPDVSDHLFYSFERGNELQLLVVNQTSGELRLSRKLDNNRPLVASMLVTVTDGLHSVTAQCVLRVVIITEELLANSLTVRLENMWQERFLSPLLGHFLEGVAAVLATPAEDVFIFNIQNDTDVGGTVLNVSFSALAPRGAGTGAAGPWFSSEELQEQLYVRRAALAARSLLDVLPFDDNVCLREPCENYMKCVSVLRFDSSAPFLASASTLFRPIQPIAGLRCRCPPGFTGDFCETELDLCYSNPCRNGGACARREGGYTCVCRPRFTGEDCELDTEAGRCVPGVCRNGGTCTDGPDGGFHCQCPAGGAFEGPRCEVAARSFPPSSFVMFRGLRQRFHLTLSISFATVQPSGLLFYNGRLNEKHDFLALELVAGQVRLTYSTGESNTVVSPTVPGGLSDGQWHTVHLRYYNKPRTDALGGAQGPSKDKVAVLSVDDCNVAVALQFGAEIGNYSCAAAGMQTSSKKSLDLTGPLLLGGVPNLPENFPVSHKDFVGCMRDLHIDGRRVDMAAFVANNGTTAGCQAKLHFCDSGPCKNSGFCSERWGGFSCDCPVGFGGRDCRLTMAYPHRFHGNGTLSWDFGNDMAVSVPWYLGLAFRTRATKGVLMQVQAGPHSALLCQLDRGLLSVTMTRGSGRAAHLLLDQVAVSDGRWHDLRLELQEEPGGRRGHHVLMVSLDFSLFQDTMAVGSELQGLKVKRLHVGGLPPSSEEESPQGLVGCIQGVWLGSTPLGSPALLAPSHRVNVEPGCVVTNACASGPCPPHADCRDLWQTFSCTCWPGYYGPGCVDACLLNPCQNQGSCRHLPGAPHGYICDCVGGYFGHHCEHRMDQQCPRGWWGSPTCGPCNCDVHKGFDPNCNKTNGQCHCKEFHYRPRDSDSCLPCDCYPVGSTSRSCAPHSGQCPCRPGALGRQCNSCDSPFAEVTASGCRVLYDACPKSLRSGVWWPQTKFGVLASVPCPRGALGAAVRLCDEDQGWLEPDLFNCTSPAFRELSLLLDGLELNKTALDTVEAKKLAQRLREVTSHTDHYFSQDIRVTARLLAHLLAFESHQQGFGLTATQDAHFNENLLWAGSALLAPETGDLWAALGQRAPGGSPGSAGLVQHLEEYAATLARNMELTYLNPVGLVTPNIMLSIDRMEHPSPTRGTRRYPRYHSNLFRGQDAWDPHTHVLLPSQAPRPSPSEVLSTSSSSIENSTTSSVAPPPAPPETEPEPGISIVILLVYRTLGGLLPAQFQAERRGARLPQNPVMNSPVVSVAVFHGRNFLRGVLESPISLEFRLLQTANRSKAICVQWDPPGPADQHGMWTARDCELVHRNGSHARCRCSRTGTFGVLMDASPRERLEGDLELLAVFTHVVVAVSVAALLLTAAILLSLRSLKSNMRGIHANVAAALGVAELLFLLGIHRTQNQLVCTAVAILLHYFFLSTFAWLLVQGLHLYRMQVEPRNVDRGAMRFYHALGWGVPAVLLGLTVGLDPEGYGNPDFCWISVYEPLIWSFAGPVVLVVVMNGTMFLLAARTSCSTGQREAKKSSVLTLRSSFLLLLLISASWLFGLLAINHSILAFYYLHAGLCGLQGLVVLLLFCVLNADARAAWTPACLGRKAVPEEARPAPGTGPGAYNNTALFEESGLIRITLGASTVSSVSSARSGRTQDQDSQPGRSYLRDNVLVRHGSAADHTDHSLQAHAGPTDLDVAMFHRDAGGGADSDSDSDLSLEEERSLSIPSSESEDNGRTRGRFQRPLRRAAQSERLLTHPKDVDGNDLLSYWPALGECEAAPCALQTWGSERRLGLDTSKDAANNNQPDLALTSGDETSLGRAQHQRKGILKNRLQYPLMPQTRGPPELSWCRAATLGHCAVPAASYGRIYAGGGTGSLSQPASRYSSREQLDLLLRRQMSRERLEEAPAPTLRPLSRPGSQECLDAVPGRLEPRDRGSTLPRRQPPRDYPSSMAGRFRSRDALDLGAPCEWLSTLPLPHSAQDLDPQPPPLPLSPQRQLSRDPLLPSRPLDSLSRRSNSGERLDHGPSRHPSREGLGPPPQLLRVREDPASGPSHGPSTEQLDILSSILASFNSSALSSSVQSSSTPSGPHTTATPSATASALGPSTPRSATSHSISELSPDSEVPRSEGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LS67</accession>
    <name>A0A8C4LS67_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Vomeronasal type-1 receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106825273</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative pheromone receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LS67"/>
    <dbReference type="Ensembl" id="ENSEAST00005013244.2">
        <property type="protein sequence ID" value="ENSEASP00005012191.2"/>
        <property type="gene ID" value="ENSEASG00005008516.2"/>
    </dbReference>
    <dbReference type="KEGG" id="eai:106825273"/>
    <dbReference type="GeneTree" id="ENSGT00960000186612"/>
    <dbReference type="OrthoDB" id="9606139at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0016503">
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0019236">
        <property type="term" value="P:response to pheromone"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007606">
        <property type="term" value="P:sensory perception of chemical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="CDD" id="cd13949">
        <property type="entry name" value="7tm_V1R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000033">
        <property type="entry name" value="Vomeronasal type-1 receptor"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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    <dbReference type="InterPro" id="IPR004072">
        <property type="entry name" value="Vmron_rcpt_1"/>
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        <property type="entry name" value="VOMERONASAL TYPE-1 RECEPTOR"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF03402">
        <property type="entry name" value="V1R"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="VOMERONASL1R"/>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="34"/>
            <end position="61"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
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            <end position="91"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="111"/>
            <end position="136"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="156"/>
            <end position="177"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="218"/>
            <end position="236"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="294"/>
            <end position="314"/>
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        <location>
            <begin position="50"/>
            <end position="314"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00003878"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="341" mass="38883" checksum="8CD13D737AB6949F" modified="2023-09-13" version="2">MIHVDRDSYPLAGFSSSEDKYLSLTTDRKASRELVVGIILSLQTTFGILGNFSLLYHYLFLYFTGSRLRSTDLIVKNLIVANLLVLLFSGIPHTMSSFGWYQVYSDFGCRFFLYFRGVGRGVSISTTCLLSVFQAIMICPRKSRRADLKEKALKCVVPSISLCWVLHMLVNVLYPMFVSSNLGNKNTTNQKSFGYCSAVRHDKTRDSLYAALLSVPDVFSLVLMMWASGSMVFILYRHKQRVKHLHRTNVSSRSSPESRATKTILLLVSTFVYFYTLSSISYVCLALFHNPNWFILKISSIISLCFPTASPFLFMSCDSSLFRLCFAWIRNTKFLNLLRNL</sequence>
</entry>
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        <submittedName>
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        <name type="scientific">Equus asinus</name>
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        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
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    <dbReference type="AlphaFoldDB" id="A0A8C4LYU4"/>
    <dbReference type="Ensembl" id="ENSEAST00005019967.2">
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    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0042296">
        <property type="term" value="F:ISG15 transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0032020">
        <property type="term" value="P:ISG15-protein conjugation"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0050688">
        <property type="term" value="P:regulation of defense response to virus"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000025">
        <property type="entry name" value="HECT and RLD domain containing E3 ubiquitin protein ligase 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000004">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000003">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051709">
        <property type="entry name" value="Ub-ligase/GTPase-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45622:SF7">
        <property type="entry name" value="E3 ISG15--PROTEIN LIGASE HERC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45622">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
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    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="4"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="RCC1" evidence="7">
        <location>
            <begin position="166"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="7">
        <location>
            <begin position="219"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="7">
        <location>
            <begin position="271"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="7">
        <location>
            <begin position="324"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="9">
        <location>
            <begin position="709"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="1001"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005018399.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1031" mass="116647" checksum="52B285F57A469107" modified="2023-09-13" version="2">MERGSRRRSRGGARSALRPAAASRPPASRPPASRSAEPPGAQLWLFPSAAGLRSALPRRTEATRQMCCTRGRLAVLERGGAGVAVHQLPAGSDGARKPKCIKLGRKMKIHSMDQGAEHLLVLSSDGKPFEYNYSIEHARFQCVLQEKNIIQITCGDYHSLALSKGGELFAWGQNTYGQLGVGRTFASTPTPQIVEHLSGVPLVQISAGEAHSMALSMSRNVYSWGRNDCGQLGLGHTNNKDSPSLIEALDNQEVEFLACGGSHTALLTKDGLVFTFGAGKCGQLGHNSTQNELTPCLVTELVGKRVTQIACGRRHTLAYVSDLGKVFSFGSGEGGQLGNGATHHQLIPLPMKLPSNEELKFESHTSEKELIMIAGGNQSILLWIEKENPYVNLRRKIPTLNEGTVKRWIADVGTKKWQNTKREIKEIFSSPACLTGSFLRERITAETMSVHLNVNKARDTFKELTQKDWITNMITTCLRDNLIKNLPFHSPHREALEVFLLLPECPVMHDYNNWESLVVPFAEAICKMSNQSLGVLEEYWASLQESAFSKLVQMFKRAIGAQLHYWSESVDSNCHVRALLEVLKRLHRVNQTNCQVPENIFEVSELTHWLDFNGDVYKRSAWKMNSDTSRDSPVVFSHFPFIFNILSKIKLLYADSLLKIWEKEHRRFMTLDGNVDPGEPELALSSSFNLTVRRSHLIEDVLNHLNRFENEDLRRELLVSFSGEIPLDFGGVRAEFFHCLFEELTQPEYGLFTYPEEASYMWFPVRPKFEKKSYFFFGVLCGLCLFNCNVANIPFPLALFKKLLDQTPSLEDLKELSPVFGRSLQTLLDDEGEDFGEVFYIHFTVHWDRNAEELIPNGSDIIVDQTNKRDYVSKCVNYIFNISIKAVYEEFQRGFYKVCDKEIIGFFHPAELKDVIIGNTDYDWETFEKNARYVGGYDSSHPTIVMFWEALHKLTLEEKKKFLVFLTGTDRLQVKGVKNMKITFCCPEHLDEKDPIRAQTCFSVLYLPKYSTMERVEEALQVAINNNRGFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M064</accession>
    <name>A0A8C4M064_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MORN repeat-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">MORN3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Assembles a suppression complex (suppresome) by tethering SIRT1 and MDM2 to regulate composite modifications of p53/TP53. Confers both deacetylation-mediated functional inactivation, by SIRT1, and ubiquitination-dependent degradation, by MDM2, of p53/TP53, promoting a proliferative and cell survival behaviors. May play a role in the regulation of spermatogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with MEIG1. Interacts with TP53, MDM2 and SIRT1; the interactions mediate post-transcriptional modifications of TP53 by MDM2 and SIRT1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Acrosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014714204.1">
        <property type="nucleotide sequence ID" value="XM_014858718.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014714205.1">
        <property type="nucleotide sequence ID" value="XM_014858719.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014714206.1">
        <property type="nucleotide sequence ID" value="XM_014858720.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044631853.1">
        <property type="nucleotide sequence ID" value="XM_044775918.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070372093.1">
        <property type="nucleotide sequence ID" value="XM_070515992.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070372094.1">
        <property type="nucleotide sequence ID" value="XM_070515993.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070372095.1">
        <property type="nucleotide sequence ID" value="XM_070515994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M064"/>
    <dbReference type="SMR" id="A0A8C4M064"/>
    <dbReference type="Ensembl" id="ENSEAST00005019411.2">
        <property type="protein sequence ID" value="ENSEASP00005017879.1"/>
        <property type="gene ID" value="ENSEASG00005012338.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106842296"/>
    <dbReference type="KEGG" id="eai:106842296"/>
    <dbReference type="CTD" id="283385"/>
    <dbReference type="GeneTree" id="ENSGT00940000159285"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901797">
        <property type="term" value="P:negative regulation of signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072331">
        <property type="term" value="P:signal transduction by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.110.10:FF:000026">
        <property type="entry name" value="MORN repeat containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.110.10">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003409">
        <property type="entry name" value="MORN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052472">
        <property type="entry name" value="MORN3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46511">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46511:SF1">
        <property type="entry name" value="MORN REPEAT-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02493">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00698">
        <property type="entry name" value="MORN"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82185">
        <property type="entry name" value="Histone H3 K4-specific methyltransferase SET7/9 N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005017879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="242" mass="28078" checksum="9C33FAACF184A40A" modified="2022-01-19" version="1">MPISKCLLKSEPLWKEWDQKAQKNGLRHQVYAVNGDCYVGEWKDNMKHGKGTQIWKKKGGIYEGDWKFGKRDGYGTFSLPDQETGKHRRVYLGWWKGDKKSGYGIQFFGPKEYYEGEWCGNQRSGWGRMYYSNGDIYEGQWCKDKPDGEGMLRLKNGNRYEGSWQRGMKNGFGRFFHLNHGQLFEGFWVDDVAKCGTMIDFGRDEAPEPTQFPIPKVKLLDPDGVLQEALARLKKTEEEEGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M0E4</accession>
    <name>A0A8C4M0E4_EQUAS</name>
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        <recommendedName>
            <fullName evidence="3">Vitamin K-dependent protein S</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">PROS1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4M0E4"/>
    <dbReference type="Ensembl" id="ENSEAST00005016979.2">
        <property type="protein sequence ID" value="ENSEASP00005015607.2"/>
        <property type="gene ID" value="ENSEASG00005010860.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154035"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042730">
        <property type="term" value="P:fibrinolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000240">
        <property type="entry name" value="Vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000426">
        <property type="entry name" value="Vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000748">
        <property type="entry name" value="Vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000077">
        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000129">
        <property type="entry name" value="Vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.740.10:FF:000001">
        <property type="entry name" value="vitamin K-dependent protein S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.740.10">
        <property type="entry name" value="Coagulation Factor IX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017857">
        <property type="entry name" value="Coagulation_fac-like_Gla_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051145">
        <property type="entry name" value="GAS-SHBG-PROS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035972">
        <property type="entry name" value="GLA-like_dom_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000294">
        <property type="entry name" value="GLA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24040">
        <property type="entry name" value="LAMININ G-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24040:SF0">
        <property type="entry name" value="VITAMIN K-DEPENDENT PROTEIN S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00594">
        <property type="entry name" value="Gla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00054">
        <property type="entry name" value="Laminin_G_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02210">
        <property type="entry name" value="Laminin_G_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00001">
        <property type="entry name" value="GLABLOOD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00069">
        <property type="entry name" value="GLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00282">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57630">
        <property type="entry name" value="GLA-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00011">
        <property type="entry name" value="GLA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50998">
        <property type="entry name" value="GLA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50025">
        <property type="entry name" value="LAM_G_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0094">Blood coagulation</keyword>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="13 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 17" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="16" id="KW-0280">Fibrinolysis</keyword>
    <keyword evidence="4" id="KW-0301">Gamma-carboxyglutamic acid</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0356">Hemostasis</keyword>
    <keyword evidence="15" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
    <feature type="domain" description="Gla" evidence="21">
        <location>
            <begin position="295"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="371"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="20">
        <location>
            <begin position="455"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin G" evidence="19">
        <location>
            <begin position="553"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="57"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="201"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="380"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="399"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005015607.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="929" mass="102388" checksum="051FFBABCA04FDDD" modified="2023-09-13" version="2">MKCNTFSFFPKTLFAFIPYMQGQDKNVDDQNYPICSILSVLCSLPGSLASRFWLTSSSPTSVSHEPSKPDALRSESSKSSWPLSPTPSPRPPGRCKCLRPSPFPLGNFTFRRDGRSAGVWDLEGKLSPQCLPGLDTLPEPAPSSEKLPGDVPLITPPGWGRERAVPSAPGPTVPRLPPGSGWPRLAPGSRCALCAACRERRPQPAAPPPAARPRPRRPPGSPPRAPARVPAERPPPPPRARVPAARLDRPRLFAMRVPRGRCAALLACLALALPVSEANFLSKQHASQVLVRKRRANSILEETKKGNLERECIEELCNKEEAREIFENIPETEYFYPKYLGCLGSFRAGLFTAARQSTNAYPDLRSCVNAIPDQCNPLPCKEDGYMSCKDGQATFTCVCKSGWQGEMCESDVNECKDPLNVNGGCSQICNNTPGSYYCSCKGGFVMLSNEKDCKDVDECTTQPSICGTAVCKNVPGDYECECAEGYSYNPSSKSCEDVDECSENMCAQLCVNYPGGYYCYCDGKKGFKLAQDQKSCEAVPVCLPLNLDKNYELLYLAEQFVGVVLYLKFRLPDITRFSAEFDFRTYDSEGVILYAESLDHSAWFLIALRDGKIEIQFKNEHTTQITTGGKVINNGLWNMVSVEELEYSISVKIAKEAVMNINKPENLFKLTNGFLETKVYFAGLPRKVENALIRPINPRLDGCIRGWNLMNQGASGVKEIIQEKQNKHCLVSVEKGSYFPGSGVAQFSINYDNTSSAEGWHVNVSLRIRPSTGTGVMFALVSGNTVPFALSLVDSTSEKLQDILVSVESMVIYQIEALSLCSNQQSYLEFRVNRDSLQVSTPLRNDVIYSEDLPRQFANLDKAMQGTMATYLGGLPDVPFSATPVNAFYSGCMDVNVNGAQLDLDEAVSKHNDIRAHSCPSVWRNTKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M0R8</accession>
    <name>A0A8C4M0R8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">E3 ubiquitin-protein ligase TRAIP</fullName>
            <ecNumber evidence="7">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">TRAF-interacting protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">TRAIP</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Interacts (via PIP-box) with PCNA. Binds TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6 is part of the receptor-TRAF signaling complex. May interact with CYLD; the C-terminus interacts with CYLD, however the interaction was not detected with the full-length protein. Interacts with POLK and POLN. Interacts with UIMC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
            <location evidence="5">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="21">Belongs to the TRAIP family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="7"/>
    <dbReference type="RefSeq" id="XP_014695938.1">
        <property type="nucleotide sequence ID" value="XM_014840452.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M0R8"/>
    <dbReference type="Ensembl" id="ENSEAST00005018533.2">
        <property type="protein sequence ID" value="ENSEASP00005017062.2"/>
        <property type="gene ID" value="ENSEASG00005011817.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106830708"/>
    <dbReference type="KEGG" id="eai:106830708"/>
    <dbReference type="CTD" id="10293"/>
    <dbReference type="GeneTree" id="ENSGT00390000007696"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090734">
        <property type="term" value="C:site of DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032688">
        <property type="term" value="P:negative regulation of interferon-beta production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010804">
        <property type="term" value="P:negative regulation of tumor necrosis factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd16480">
        <property type="entry name" value="RING-H2_TRAIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000431">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRAIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052639">
        <property type="entry name" value="TRAIP_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46569:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RFWD3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46569">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRAIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0158">Chromosome</keyword>
    <keyword evidence="18 26" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="19" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="16" id="KW-0862">Zinc</keyword>
    <keyword evidence="14 25" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="28">
        <location>
            <begin position="7"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="345"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="26">
        <location>
            <begin position="76"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="26">
        <location>
            <begin position="194"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00061509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00065918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00068429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00079945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005017062.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="469" mass="53267" checksum="9329626E696FD845" modified="2023-09-13" version="2">MPIRALCTICSDFFDHSRDVAAIHCGHTFHLQCLIQWFETAPSRTCPQCRIQVGRRTIINKLFFDLAQEEESVLDAEFLKNELDNTRAQLSQKEKEKRDSQVIIDALRDTLEERNATVESLQRALDKAEMLCSTLKKQMKYLEQQQDETKQAREEACRLRRKMKTMERIELLLQSQRPEVEEMIRDMGVGQSAVEQLAVYCVSLKKEYENLKEARKASGELADKLKKDLFSSRSKLQKVYSELDQTKLELRSAQKDLQSADKEIVSLKKKLTMLQETLNLPPVDSETVNRLVLESPAPVEVLNLKLRRPAFSDDIDLNATFDVATPPGRPSSIQHGHTKKLCPEKAHSPVQDIPKKMPKGPKQESQLSLGGQRCVGEPDEELAGAFPVFIRNAVLGQKQLKRARVEPCRNTDAVRTGFDGLGGRTKFIQPTDTTMIRPLPVKPKAKAKQRIAARTVLPPSQAKLDNFLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M2L2</accession>
    <name>A0A8C4M2L2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="35 36">Poly [ADP-ribose] polymerase</fullName>
            <ecNumber evidence="35">2.4.2.30</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="43" type="primary">PARP1</name>
    </gene>
    <organism evidence="43 44">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="43 44" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="43" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="43" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="35">Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair.</text>
    </comment>
    <comment type="function">
        <text evidence="31">Promotes AIFM1-mediated apoptosis. This form, which translocates into the cytoplasm following cleavage by caspase-3 (CASP3) and caspase-7 (CASP7) in response to apoptosis, is auto-poly-ADP-ribosylated and serves as a poly-ADP-ribose carrier to induce AIFM1-mediated apoptosis.</text>
    </comment>
    <comment type="function">
        <text evidence="29">This cleavage form irreversibly binds to DNA breaks and interferes with DNA repair, promoting DNA damage-induced apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>L-aspartyl-[protein] + NAD(+) = 4-O-(ADP-D-ribosyl)-L-aspartyl-[protein] + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:54424"/>
            <dbReference type="Rhea" id="RHEA-COMP:9867"/>
            <dbReference type="Rhea" id="RHEA-COMP:13832"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29961"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:138102"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:54425"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>L-glutamyl-[protein] + NAD(+) = 5-O-(ADP-D-ribosyl)-L-glutamyl-[protein] + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:58224"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:15089"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:142540"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:58225"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>L-histidyl-[protein] + NAD(+) = N(tele)-(ADP-D-ribosyl)-L-histidyl-[protein] + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:72071"/>
            <dbReference type="Rhea" id="RHEA-COMP:9745"/>
            <dbReference type="Rhea" id="RHEA-COMP:18085"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:191398"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:72072"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>L-seryl-[protein] + NAD(+) = O-(ADP-D-ribosyl)-L-seryl-[protein] + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:58232"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:15091"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:142556"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:58233"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>L-tyrosyl-[protein] + NAD(+) = O-(ADP-D-ribosyl)-L-tyrosyl-[protein] + nicotinamide + H(+)</text>
            <dbReference type="Rhea" id="RHEA:58236"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:15092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:142557"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:58237"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28 35">
            <text>NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D-ribosyl)n+1-acceptor + H(+).</text>
            <dbReference type="EC" id="2.4.2.30"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="30">Interacts (when auto-poly-ADP-ribosylated) with AIFM1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 35">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="27">Belongs to the ARTD/PARP family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.30" evidence="35"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4M2L2"/>
    <dbReference type="Ensembl" id="ENSEAST00005020959.2">
        <property type="protein sequence ID" value="ENSEASP00005019314.2"/>
        <property type="gene ID" value="ENSEASG00005013275.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156058"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:nucleotidyltransferase activity"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="term" value="P:cellular response to oxygen-containing compound"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="P:innate immune response"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="parp_like"/>
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        <property type="entry name" value="WGR_PARP1_like"/>
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        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
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        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
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        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
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        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
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        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
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        <property type="entry name" value="Poly [ADP-ribose] polymerase"/>
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    <dbReference type="Gene3D" id="1.10.20.130">
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.20.25.630">
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
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    <dbReference type="Gene3D" id="1.20.142.10">
        <property type="entry name" value="Poly(ADP-ribose) polymerase, regulatory domain"/>
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        <property type="entry name" value="Zinc finger, PARP-type"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR050800">
        <property type="entry name" value="ARTD/PARP"/>
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        <property type="entry name" value="BRCT_dom"/>
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    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
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        <property type="entry name" value="PADR1_C_dom_sf"/>
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    <dbReference type="InterPro" id="IPR008288">
        <property type="entry name" value="PARP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049296">
        <property type="entry name" value="PARP1-like_PADR1_N"/>
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    <dbReference type="InterPro" id="IPR012982">
        <property type="entry name" value="PARP1-like_PADR1_Zn_ribbon"/>
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    <dbReference type="InterPro" id="IPR012317">
        <property type="entry name" value="Poly(ADP-ribose)pol_cat_dom"/>
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    <dbReference type="InterPro" id="IPR004102">
        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom"/>
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        <property type="entry name" value="Poly(ADP-ribose)pol_reg_dom_sf"/>
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        <property type="entry name" value="WGR_dom_sf"/>
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        <property type="entry name" value="Znf_PARP"/>
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    <dbReference type="InterPro" id="IPR036957">
        <property type="entry name" value="Znf_PARP_sf"/>
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    <dbReference type="PANTHER" id="PTHR10459">
        <property type="entry name" value="DNA LIGASE"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10459:SF112">
        <property type="entry name" value="POLY [ADP-RIBOSE] POLYMERASE 1"/>
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    <dbReference type="SMART" id="SM00773">
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    <dbReference type="SUPFAM" id="SSF56399">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52113">
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    <dbReference type="SUPFAM" id="SSF47587">
        <property type="entry name" value="Domain of poly(ADP-ribose) polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
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        <property type="entry name" value="WGR domain-like"/>
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    <dbReference type="PROSITE" id="PS50172">
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    </dbReference>
    <dbReference type="PROSITE" id="PS52007">
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    </dbReference>
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        <property type="entry name" value="PARP_ALPHA_HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51059">
        <property type="entry name" value="PARP_CATALYTIC"/>
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    </dbReference>
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        <property type="entry name" value="WGR"/>
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    </dbReference>
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        <property type="entry name" value="ZF_PARP_1"/>
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    </dbReference>
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        <property type="entry name" value="ZF_PARP_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="7" id="KW-0021">Allosteric enzyme</keyword>
    <keyword evidence="4 35" id="KW-0158">Chromosome</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="23" id="KW-0234">DNA repair</keyword>
    <keyword evidence="21 35" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9 35 36" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="18" id="KW-0391">Immunity</keyword>
    <keyword evidence="8" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12 35" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20 35 36" id="KW-0520">NAD</keyword>
    <keyword evidence="11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="24 35" id="KW-0539">Nucleus</keyword>
    <keyword evidence="44" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="22" id="KW-0804">Transcription</keyword>
    <keyword evidence="19" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10 35 36" id="KW-0808">Transferase</keyword>
    <keyword evidence="17 35" id="KW-0862">Zinc</keyword>
    <keyword evidence="16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PARP-type" evidence="38">
        <location>
            <begin position="9"/>
            <end position="93"/>
        </location>
    </feature>
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        <location>
            <begin position="113"/>
            <end position="203"/>
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        <location>
            <begin position="385"/>
            <end position="461"/>
        </location>
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        <location>
            <begin position="542"/>
            <end position="638"/>
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        <location>
            <begin position="662"/>
            <end position="779"/>
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    <feature type="domain" description="PARP catalytic" evidence="40">
        <location>
            <begin position="788"/>
            <end position="1009"/>
        </location>
    </feature>
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        <location>
            <begin position="197"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="37">
        <location>
            <begin position="360"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="37">
        <location>
            <begin position="493"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="37">
        <location>
            <begin position="943"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="37">
        <location>
            <begin position="204"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="37">
        <location>
            <begin position="493"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="37">
        <location>
            <begin position="510"/>
            <end position="521"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
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            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
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            <dbReference type="ARBA" id="ARBA00023015"/>
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    <evidence type="ECO:0000256" key="20">
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            <dbReference type="ARBA" id="ARBA00023027"/>
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    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
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    <evidence type="ECO:0000256" key="23">
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    <evidence type="ECO:0000256" key="24">
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00048241"/>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="35">
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            <dbReference type="PIRNR" id="PIRNR000489"/>
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    </evidence>
    <evidence type="ECO:0000256" key="36">
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            <dbReference type="RuleBase" id="RU362114"/>
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    </evidence>
    <evidence type="ECO:0000256" key="37">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="38">
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    <evidence type="ECO:0000259" key="39">
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    <evidence type="ECO:0000259" key="40">
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    </evidence>
    <evidence type="ECO:0000259" key="41">
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="42">
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    <evidence type="ECO:0000313" key="43">
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    </evidence>
    <evidence type="ECO:0000313" key="44">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1014" mass="112867" checksum="F12DC8BA7F406BB2" modified="2023-09-13" version="2">MAESSDKLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGHSIRHPDVEVDGFSELRWDDQQKVKKTAEAGGVAGKGQGGSGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQMRLSKKMLDPEKPQLGMIDRWYHPNCFVKNREELGFRPEYSASQLKGFSLLSPEDKEALKKQLPGVKTEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKACSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKIKKQDRIFPPETSAPAAAAPPPSAVSAPATVNASALPDKPLSNMKILTLGKLSRNKDEVKATIEKLGGKLTGTASKASLCISTQKEVEKMNKKMEEVKEANLCVVSEDFLQDVSTSTKSLQELLSAHILSPWGAEVKAEPVEVAAPRGKSGAALSKKSKGPVKEEGSNKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKESRYWIFRSWGRVGTVIGSNKREQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDYGQDEEAVKKLTLNPGTKSKLPKPVQELIKMIFDVESMKKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQALSQGSGDSQILDLSNRFYTLIPHDFGMKKPPLLNNTDSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDKDSEEAETIRKYVKNTHATTHNAYDLEVVDIFKIEREGESQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSASVTMDGVEVPLGTGISSGVSDTCLLYNEYIVYDIAQEPSWNHHLGNCWYFMESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M2R1</accession>
    <name>A0A8C4M2R1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">ADAM metallopeptidase domain 12</fullName>
        </submittedName>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
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    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="component" value="Chromosome 2"/>
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        <property type="entry name" value="Reprolysin_adamalysin"/>
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<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
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                <person name="Tian"/>
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                <person name="Gao"/>
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                <person name="Liu"/>
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                <person name="Guo"/>
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                <person name="Jin"/>
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                <person name="Xu"/>
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                <person name="Liu"/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Microtubule inner protein component of sperm flagellar doublet microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the CIMIP2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4M2Y1"/>
    <dbReference type="Ensembl" id="ENSEAST00005021999.2">
        <property type="protein sequence ID" value="ENSEASP00005020261.2"/>
        <property type="gene ID" value="ENSEASG00005013951.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154822"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005879">
        <property type="term" value="C:axonemal microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018902">
        <property type="entry name" value="CMI2A-C-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22146">
        <property type="entry name" value="CAT EYE SYNDROME CRITICAL REGION PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22146:SF8">
        <property type="entry name" value="PROTEIN FAM166B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10629">
        <property type="entry name" value="CMI2B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0966">Cell projection</keyword>
    <keyword evidence="3" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ciliary microtubule inner protein 2A-C-like" evidence="11">
        <location>
            <begin position="15"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="125"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="134"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="156"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020261.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="282" mass="31742" checksum="F7767B9E708462DA" modified="2023-09-13" version="2">GTMASTFTLGLNPQHPHHIPGYTGHCPLFRFSMGQAYGQITGHLLRGSPGLAWPPAHRTLLPPIWPPRSPEVPRRRSLRVRHGHERLSSSMIPGYTGFVPQAQFIFAKNCSQVWAEALHHFTQWHGGQGSQEQLPKEAKGEKDMGKDQEPTLEPELEAKEEPQLGEKVEQASPYSMDDRDPRKFFMSGFTGYVPHACFLFSSSFPVLTNQALQEFGQMYSWGRPQKDPKHLLPLSMTFPQNLGLLPNYGGSVPRCKFQFGHTSWHLTHDALGLSTLQRQLLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M5Z4</accession>
    <name>A0A8C4M5Z4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">RIMS-binding protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">RIMBP2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Plays a role in the synaptic transmission as bifunctional linker that interacts simultaneously with RIMS1, RIMS2, CACNA1D and CACNA1B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RIMBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4M5Z4"/>
    <dbReference type="Ensembl" id="ENSEAST00005020824.2">
        <property type="protein sequence ID" value="ENSEASP00005019188.2"/>
        <property type="gene ID" value="ENSEASG00005013193.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183203"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007274">
        <property type="term" value="P:neuromuscular synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="CDD" id="cd12014">
        <property type="entry name" value="SH3_RIM-BP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12012">
        <property type="entry name" value="SH3_RIM-BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12013">
        <property type="entry name" value="SH3_RIM-BP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000023">
        <property type="entry name" value="RIMS-binding protein 2 isoform F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000006">
        <property type="entry name" value="RIMS-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000072">
        <property type="entry name" value="RIMS-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000643">
        <property type="entry name" value="RIMS-binding protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000016">
        <property type="entry name" value="RIMS-binding protein 2 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057884">
        <property type="entry name" value="FN3_RIM-BP1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035753">
        <property type="entry name" value="RIM-BP_SH3_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035755">
        <property type="entry name" value="RIM-BP_SH3_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040325">
        <property type="entry name" value="RIMBP1/2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14234">
        <property type="entry name" value="RIM BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14234:SF18">
        <property type="entry name" value="RIMS-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25523">
        <property type="entry name" value="Ig_RIMBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07653">
        <property type="entry name" value="SH3_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="167"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="15">
        <location>
            <begin position="489"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="856"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="14">
        <location>
            <begin position="960"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="125"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="585"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="633"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="701"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="805"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1037"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="588"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="650"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="676"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="716"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="741"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1048"/>
            <end position="1060"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005019188.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1060" mass="116727" checksum="14A995F2CAB9BACD" modified="2023-09-13" version="2">MREAAERRQQLELEHEQALAVLNAKQQEIDLLQKSKVRELEEKCRTQSEQFSLLSRDLEKFRQHAGKIDLLGGSSVASLDVPTSPSKPFPPYMNGLAPSISKGQESSIGSRAAIGEYIRPLPLPRDKPEPLSVKPTFLSRSSSPRCRFEPDMDNERNSNTSKQRYSGKVHLCVARYSYNPFDGPNENPEAELPLTAGKYLYVYGDMDEDGFYEGELLDGQRGLVPSNFVDFVQDNESRLASALGNEQDQNFINHSSFGVEGESILDLHSPTQTDTGITDNGAGTLDMNIDDIGEDIVPYPRKITLIKQLAKSVIVGWEPPAVPPGWGTVSSYNVLVDKETRLSLTLGGRTKALIEKLNMAACTYRISVQCMTSRGSSDELQCTLLVGKDVVVAPSHLRVDNITQISAQLSWLPTNSNYSHVIFLNEEEFDIVKAARYKYQFFNLRPNMAYKVKVLAKPHQMPWQLPLEQREKQEAFVEFSTLPAGPPAPPQDVTVQAGASSATVQVSWRPPTLTTTGLSNGANVTGYGVYAKGHRVAEVISPTADSTAVELVRLRSLEAKGVTVRTLSAQGESIDSAVAAIPADLLVPPTPHPRTAPTPKPLASAGAPDTKDEHLGPHVKVDEAWEQSRVPAPAHGHMLEPPGLQGPGPGRRSPSPSRILPQPQGTPVSTSVAKAMAREAAQRVAESSRLEKRSIFLERSSGQYTNSDEEDGYDSPDVKRRGASVDDFLKGSELGKPPHCCHGDEYHTESSRGSDLSDIMEEDEEELYSEMQLEDGGRRRPSGTSHNALKILGNPVLAGRAAERADHGGRRFSHGGAGPQRSRPVMVPSIDEYGGRDRLSPDFYEESETDPGAEELPARIFVALFDYDPLTMSPNPDAAEEELPFKEGQIIKVYGDKDADGFYRGETCARLGLIPCNMVSEIQADDEEMVDQLLRQGFLPLNTPVEKIERNRRSGRQHSVSTRRMVALYDYDPRESSPNIDVEAELTFCTGDIITVFGEIDEDGFYYGELNGQKGLVPSNFLEEVPDDVEVYLSDAPSHYSQDTPMRSKAKRKKSVHFTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M755</accession>
    <name>A0A8C4M755_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ski oncogene</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Proto-oncogene c-Ski</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">SKI</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SKI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044625432.1">
        <property type="nucleotide sequence ID" value="XM_044769497.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M755"/>
    <dbReference type="Ensembl" id="ENSEAST00005022855.2">
        <property type="protein sequence ID" value="ENSEASP00005021064.2"/>
        <property type="gene ID" value="ENSEASG00005014420.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106845212"/>
    <dbReference type="CTD" id="6497"/>
    <dbReference type="GeneTree" id="ENSGT00940000160546"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046332">
        <property type="term" value="F:SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030512">
        <property type="term" value="P:negative regulation of transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21083">
        <property type="entry name" value="DHD_Ski"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.390.10:FF:000002">
        <property type="entry name" value="Putative ski oncogene"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.20:FF:000002">
        <property type="entry name" value="SKI-like oncogene a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.390.10">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.20">
        <property type="entry name" value="Ski"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014890">
        <property type="entry name" value="c-SKI_SMAD4-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047315">
        <property type="entry name" value="DHD_Ski"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010919">
        <property type="entry name" value="SAND-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003380">
        <property type="entry name" value="SKI/SNO/DAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037000">
        <property type="entry name" value="Ski_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023216">
        <property type="entry name" value="Tscrpt_reg_SKI_SnoN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10005:SF15">
        <property type="entry name" value="SKI ONCOGENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10005">
        <property type="entry name" value="SKI ONCOGENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08782">
        <property type="entry name" value="c-SKI_SMAD_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02437">
        <property type="entry name" value="Ski_Sno_DHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01046">
        <property type="entry name" value="c-SKI_SMAD_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63763">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="c-SKI SMAD4-binding" evidence="5">
        <location>
            <begin position="217"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="318"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="453"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="708"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="334"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="461"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="474"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="493"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="525"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00032146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005021064.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="731" mass="80525" checksum="8CDD61CE46A2DE15" modified="2023-09-13" version="2">MEAAAGGRGGFQPHPGLQKTLEQFHLSSMSSLGGPAAFSARWAQEAYKKESAKEAGAAAVPAPVPAAAEPPPVLHLPAIQPPPPVLPGPFFMPSDRSTERCETVLEGETISCFVVGGEKRLCLPQILNSVLRDFSLQQINSVCDELHIYCSRCTADQLEILKVMGILPFSAPSCGLITKTDAERLCNALLYGGAYPPPCKKELAASLALGLELSERSVRVYHECFGKCKGLLVPELYSSPSAACIQCLDCRLMYPPHKFVVHSHKALENRTCHWGFDSANWRAYILLSQDYTGKEEQTRLGRCLDDVKEKFDYGNKYKRRVPRQVSSEPPASIRKTDDTPSQHPTSSEKDKQSGWLRTLASPSSKSLGCVHPRQRLSAFRPWSPAVSTSDKELSPHLPALIRDSFYSYKSFETGVAPNVALAPPAQQKVVSSPPCATVVSRAPEPLATCVQPRKRKLAVDTPGAPETPVPVAAPEDDKDSEAEVEVESREEFTSSLSSLSSPSFTSSSSAKDLSSPGVHVPPVAPAAADAAAPADTPSSGLEAELEHLRQALEGGLDTKEAKEKFLHEVVKMRVKQEEKLSAALQAKRSLHQELEFLRVAKKEKLREATEAKRNLRKEIERLRAENEKKMKEANESRLRLKRELEQARQVRVCDKGCEAGRLRAKYSAQIEDLQVKLQHAEADREQLRADLLREREAREHLEKVVKELQEQLWPRPRSEATGGESTAELEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M9T6</accession>
    <name>A0A8C4M9T6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lipocalin/cytosolic fatty-acid binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106833145</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4M9T6"/>
    <dbReference type="Ensembl" id="ENSEAST00005024617.2">
        <property type="protein sequence ID" value="ENSEASP00005022689.2"/>
        <property type="gene ID" value="ENSEASG00005015472.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244868"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
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        <property type="term" value="F:odorant binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:small molecule binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
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    <dbReference type="InterPro" id="IPR002345">
        <property type="entry name" value="Lipocalin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002448">
        <property type="entry name" value="OBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11430:SF89">
        <property type="entry name" value="ALLERGEN BOS D 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11430">
        <property type="entry name" value="LIPOCALIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01173">
        <property type="entry name" value="ODORANTBNDNG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040415125" description="Lipocalin/cytosolic fatty-acid binding domain-containing protein" evidence="4">
        <location>
            <begin position="16"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="5">
        <location>
            <begin position="30"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005022689.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="173" mass="19565" checksum="0331448E6B5C55E2" modified="2023-09-13" version="2">MKILLLTLVLGVVCADQEPQSETDYSLTPGEWYTTSIASDDTEKIQENGPLRGYSRRIECISNCSTVAITFYQKINGECRKYIVVGTSTKDDIYKVDFLGENYFQFIFKSSDSLVIYGENVDDEGKVTLVTELLAKEHSVSEQDYQKFKELTEEKGIPAENIEDIINTDDCPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MA81</accession>
    <name>A0A8C4MA81_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitotic checkpoint protein BUB3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">BUB3</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="16">Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. Interacts with ZNF207/BuGZ; leading to promote stability and kinetochore loading of BUB3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Centromere</location>
            <location evidence="2">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the WD repeat BUB3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MA81"/>
    <dbReference type="Ensembl" id="ENSEAST00005021301.2">
        <property type="protein sequence ID" value="ENSEASP00005019635.2"/>
        <property type="gene ID" value="ENSEASG00005013498.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183091"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000047">
        <property type="entry name" value="Mitotic checkpoint protein bub3, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="13" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="9" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="11" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 17" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="17">
        <location>
            <begin position="92"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="17">
        <location>
            <begin position="237"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005019635.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="328" mass="37226" checksum="2FDA137887368E5A" modified="2023-09-13" version="2">MTGSNEFKLNQPPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMRLKYQHTGAVLDCAFYDPTHAWSGGLDHQLKMHDLNTDQENLVGTHDAPIRCVEYCPEVNVMVTGSWDQTVKLWDPRTPCNAGTFSQPEKVYTLSVSGDRLIVGTAGRRVLVWDLRNMGYVQQRRESSLKYQTRCIRAFPNKQGYVLSSIEGRVAVEYLDPSPEVQKKKYAFKCHRLKENNIEQIYPVNAISFHNIHNTFATGGSDGFVNIWDPFNKKRLCQFHRYPTSIASLAFSNDGTTLAIASSYMYEMDDTEHPEDGIFIRQVTDAETKPKPKHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MC42</accession>
    <name>A0A8C4MC42_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Nucleolar protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NOL11</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MC42"/>
    <dbReference type="Ensembl" id="ENSEAST00005025314.2">
        <property type="protein sequence ID" value="ENSEASP00005023327.1"/>
        <property type="gene ID" value="ENSEASG00005015876.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009760"/>
    <dbReference type="OMA" id="QGTTGQC"/>
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        <property type="component" value="Chromosome 13"/>
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        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901838">
        <property type="term" value="P:positive regulation of transcription of nucleolar large rRNA by RNA polymerase I"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042859">
        <property type="entry name" value="NOL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048897">
        <property type="entry name" value="Nol11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012584">
        <property type="entry name" value="NOL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15633">
        <property type="entry name" value="NUCLEOLAR PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15633:SF2">
        <property type="entry name" value="NUCLEOLAR PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20998">
        <property type="entry name" value="Nol11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08168">
        <property type="entry name" value="NOL11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Nucleolar protein 11 N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleolar protein 11 C-terminal" evidence="8">
        <location>
            <begin position="399"/>
            <end position="686"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF20998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005023327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="696" mass="77934" checksum="5872BE5F327FF55A" modified="2022-01-19" version="1">MAALEEGFTLSAVCLGAGPDGLLGVEQSDKTDQFLVTDSGRTVILYKVSDQKPLGSWSVKQGQIITCPAVCNFQTGEYVVVHDSKVLRIWNNEDVNLDKVFKATLSAEVYRIHSIQGTEPLVLFKEGAVRGLEALLAEPQQKIETVISDEEVIKWTKVFMVFRHPVLIFITEKCGNYFAYVQMFNSNILSKYTLLLGQEEKSIIQSFTASVDRKFISLMSLTSDGCIYETLIPIRPNDPEKNQRVVRSLLLKAVVSGNARNGVTLTVLDQDHVAVLGPPLPASKECLAIWNTKFQTLQASKELPQGTSGQLWYYGENLFMLHGKFLTVIPYKCEVSSLAGALGKLKHSQDLGTHAMPHFVNWETSQGCGLGFQDSEPSKRILRRRKIEVSVEPEYPASKQLLSTVKKDSEKHIEVEVRKFLAVKRTPDFQIVTGDIVTGLVGRCRAEPSFYPRSALMQLIQTHVLSYSLCPALMEVALEKTDVQMLQLCLQQFPDVPESVICTCLKIFLSIGDDSLQETDINMESVSNYSDTVQDEKMEEQTEIVQNGFSPEEDNCGQEKSQAAAEEATSCPVIPKRAALLNAILHSAYSETFLLPHLKDIPAQHITLFLQYLYFLYLKCSENATMTLPGIHPPTLNQIMDWICLLLDANFTVVVMIPEAKRLLLSLYKFVKSQICICSELNKIEARDMFCLRLCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8C4MDS3_EQUAS</name>
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        <submittedName>
            <fullName evidence="2">Coiled-coil domain containing 198</fullName>
        </submittedName>
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        <name evidence="2" type="primary">CCDC198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_014706210.1">
        <property type="nucleotide sequence ID" value="XM_014850724.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MDS3"/>
    <dbReference type="Ensembl" id="ENSEAST00005025993.2">
        <property type="protein sequence ID" value="ENSEASP00005023964.1"/>
        <property type="gene ID" value="ENSEASG00005016260.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837264"/>
    <dbReference type="KEGG" id="eai:106837264"/>
    <dbReference type="CTD" id="55195"/>
    <dbReference type="GeneTree" id="ENSGT00390000001071"/>
    <dbReference type="OMA" id="QRDHKAK"/>
    <dbReference type="OrthoDB" id="6344011at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097009">
        <property type="term" value="P:energy homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029235">
        <property type="entry name" value="FAME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16065">
        <property type="entry name" value="COILED-COIL DOMAIN CONTAINING 198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16065:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN CONTAINING 198"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15398">
        <property type="entry name" value="DUF4619"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005023964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="296" mass="34463" checksum="7E464151DA20CE8E" modified="2022-01-19" version="1">MGLSHSRAHPRVTKVAPLQNKEEEPPSAGPVDIVFNQKLEEKSSYLFTRLQDRSKALEGQLPPLRETWYGRYSAVPRAMYFDIPLEQGETSIIKRHPPRRLQKLEPVDLPHVITSERLLSQQEAWTAHKAKQESEKKMQTPMHTSGKRQYLHKMQMLEMNRKRQEAQVELKKSLHREARINKQNLRDHKAKKILQSIPINDGGELLTLLPDETLNRSLGNSQNAELLEHQARNDCCPRKIGKMETWLREQEARGQLLWDSSSSDSDEVGKVEKKPRALVRTRTERIPLFDEFFDRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4ME41</accession>
    <name>A0A8C4ME41_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="20">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">GALNT9</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Does not glycosylate apomucin or SDC3.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23956"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12788"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:53604"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52424"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="ChEBI" id="CHEBI:87075"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 20">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 20">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 20">Golgi apparatus membrane</location>
            <topology evidence="2 20">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="20"/>
    <dbReference type="RefSeq" id="XP_014716587.2">
        <property type="nucleotide sequence ID" value="XM_014861101.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4ME41"/>
    <dbReference type="Ensembl" id="ENSEAST00005026637.2">
        <property type="protein sequence ID" value="ENSEASP00005024558.2"/>
        <property type="gene ID" value="ENSEASG00005016684.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106843871"/>
    <dbReference type="KEGG" id="eai:106843871"/>
    <dbReference type="CTD" id="50614"/>
    <dbReference type="GeneTree" id="ENSGT00940000156599"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
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        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000192">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF28">
        <property type="entry name" value="POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 20" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12 20" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 20" id="KW-0430">Lectin</keyword>
    <keyword evidence="16 20" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B lectin" evidence="21">
        <location>
            <begin position="466"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00052209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00057231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005024558.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="604" mass="68584" checksum="DA7CFCA409A2CF1D" modified="2023-09-13" version="2">MAVARKIKTLLTVNILVFVGIILFSVYCRLQDRSEGLVQIVRSADRRVRSRQAKVGALVDREAILQRLDHLEEVVYNQLNGLAKPIGLVEGPGGLGQGGMAATLRDDSQDTESKFEEYGYNAQLSDRISLDRTIPDYRPQKCRQMTYSDDLPQISVVFIFVNEALSVILRSVHSVVNHTPSQLLKEVILVDDDSDNVELKFNLDQYVNKRYPGLVKIVRNSRREGLIRARLQGWKAATAPVVGFFDAHVEFSTGWAEPALTRIREDRRRIVLPAIDNIKYDTFEVQQYASAAHGYNWGLWCMYIIPPQDWLDRGDEAAPIRTPAMIGCSFVVDREYFGDIGLLDPGMEVYGGENVELGMRVWQCGGSMEVLPCSRVAHIERTRKPYNNDIDYYAKRNALRAAEVWMDGFKSHVYMAWNIPMTNPGVDFGDVSERLALRQRLKCRSFKWYLENVYPEMRTYNNTLTYGEVRNSKASGYCLDQGAEDDDRAILYPCHGMSSQLVRYSADGLLQLGPLGSTSFLPDSKCLVDDGRGRVPALRKCEDVARPAQRLWDFTQSGPIVSRDTGRCLEVEMSKDANFGLRLVVQRCSGQKWTIRNWIKHGRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MH05</accession>
    <name>A0A8C4MH05_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Sterol regulatory element-binding protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Sterol regulatory element-binding transcription factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">SREBF1</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="26">Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis and lipid homeostasis. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Regulates the promoters of genes involved in cholesterol biosynthesis and the LDL receptor (LDLR) pathway of sterol regulation.</text>
    </comment>
    <comment type="function">
        <text evidence="25">Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 1), which is embedded in the endoplasmic reticulum membrane. Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis and lipid homeostasis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="27">Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with CEBPA, the interaction produces a transcriptional synergy. Interacts with LMNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="28">Forms a tight complex with SCAP, the SCAP-SREBP complex, in the endoplasmic reticulum membrane and the Golgi apparatus. Interacts with PAQR3; the interaction anchors the SCAP-SREBP complex to the Golgi apparatus in low cholesterol conditions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">COPII-coated vesicle membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the SREBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MH05"/>
    <dbReference type="Ensembl" id="ENSEAST00005028092.2">
        <property type="protein sequence ID" value="ENSEASP00005025877.2"/>
        <property type="gene ID" value="ENSEASG00005017661.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159156"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012507">
        <property type="term" value="C:ER to Golgi transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004879">
        <property type="term" value="F:nuclear receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032810">
        <property type="term" value="F:sterol response element binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001221">
        <property type="term" value="F:transcription coregulator binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006695">
        <property type="term" value="P:cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007623">
        <property type="term" value="P:circadian rhythm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045444">
        <property type="term" value="P:fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008286">
        <property type="term" value="P:insulin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030073">
        <property type="term" value="P:insulin secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042789">
        <property type="term" value="P:mRNA transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046676">
        <property type="term" value="P:negative regulation of insulin secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090209">
        <property type="term" value="P:negative regulation of triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045542">
        <property type="term" value="P:positive regulation of cholesterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902895">
        <property type="term" value="P:positive regulation of miRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010867">
        <property type="term" value="P:positive regulation of triglyceride biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019217">
        <property type="term" value="P:regulation of fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003062">
        <property type="term" value="P:regulation of heart rate by chemical signal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901524">
        <property type="term" value="P:regulation of mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903214">
        <property type="term" value="P:regulation of protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009749">
        <property type="term" value="P:response to glucose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032933">
        <property type="term" value="P:SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd18921">
        <property type="entry name" value="bHLHzip_SREBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000016">
        <property type="entry name" value="Sterol regulatory element-binding transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46062">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46062:SF2">
        <property type="entry name" value="STEROL REGULATORY ELEMENT-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0010">Activator</keyword>
    <keyword evidence="5" id="KW-0153">Cholesterol metabolism</keyword>
    <keyword evidence="29" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="21" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="19" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="18" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="17" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BHLH" evidence="31">
        <location>
            <begin position="322"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="39"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="150"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="395"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="29">
        <location>
            <begin position="362"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="30">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="30">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="30">
        <location>
            <begin position="430"/>
            <end position="461"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00038460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00039749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00042215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00045313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00045371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00047005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00049702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025877.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1149" mass="121640" checksum="B8B28A10098F1FF8" modified="2023-09-13" version="2">MDEPPFSEVALEQALAEPCELDAALLTDIEDMLQLINNQDSDFPGLFDPPYAGGREGGTDPASPDVSSPGSLSPSPAMMSSPLEGFLGGPKATPPPLSPPPPAPTPLKMYPSVPAFSPSPGIKEEPVPLTVLQPATPQPLPGGLLPQSFSAPAPPQFSSAPVLGYPSPSGGFSTGSPPGSTLQPLPGLPLASLPAVPPGSLQTQVQSTAPQQLLTATATPTVAPGTTTVTSQIQQVPVLLQPHFIKAESLLLTTVKTDVGVKAASISSLAPGTAVQTGPLQTLVSGGTILATVPLVVDPDKLPINRLAAGGKAPGSAQSRGEKRTAHNAIEKRYRSSINDKIVELKDLVVGTEAKLNKSAVLRKAIDYIRFLQQSNQKLKQENLSLRTAAHKSKSLKDLASACSRGGNTDTPMEGMKPEVVDTLSPPPSDAGSPSRSSPLSLGSRSSGSRSSGSDSEPDSPVFEDSQVKPEQLPSPHSRGMLDRSRLALCTLVFLCLSCNPLASLLGSWGLPGTSDATSMNHRPGRNMLGTEGRGRTDGPGWSPWLLPPLIWLTNGLLVLVSLTLLFVYGEPVTRPHSGPAVRFWRHRKQADLDLARGDFAQATQQLWLALRALGRPLPTSHLDLACSLLWNLIRHLLQRLWVGRWLAGRAGGLQRDCALQADARASAREAALVYHQLHQLHTMGKYSGGPLAAASLALSAVNLGECAGDAVSTATLAEIYVAAALRVKTSLPRALHFLTRFFLSSARQACLAQSGSVPLAMQWLCHPVGHRFFVDGDWAVCSAPRESLYSVAGNPVDPLAQVTQLFREHLLERALNCVAQPNPSPGSADGDREFSDALGYLQLLNSCSDAAGTPACSFSISSSMATTTGTDPVAKWWASLTAVVTHWLRQDEEAAERLYPLVEHLPRALQESERPLPRAALHSVKAARALLGRGKADSGPASLALCEKASGYLQDSLATTPAGNSIDKAMQLLLCDLLLVARTSLWRRQQLPAPAQASQGPGSGAQASALELHGFQRDLSGLRRLAQSFRPAMRRVFLHEATARLMAGASPTRTHQLLDRSLRRRAGPCGKGGAAAELEPRPTRRERAEALLLASCYLPPGFLSAPGQRVGMLAEAARTLEKLGDRRLLHDCQQMLLRLGGGTTVTSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MI72</accession>
    <name>A0A8C4MI72_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15 18">Solute carrier family 2, facilitated glucose transporter member 3</fullName>
            <shortName evidence="18">GLUT-3</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Glucose transporter type 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SLC2A3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Facilitative glucose transporter that can also mediate the uptake of various other monosaccharides across the cell membrane. Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate. Does not mediate fructose transport.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Facilitative glucose transporter. Can also mediate the uptake of various other monosaccharides across the cell membrane. Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate. Does not mediate fructose transport. Required for mesendoderm differentiation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>D-galactose(in) = D-galactose(out)</text>
            <dbReference type="Rhea" id="RHEA:34915"/>
            <dbReference type="ChEBI" id="CHEBI:4139"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18">
            <text>D-glucose(out) = D-glucose(in)</text>
            <dbReference type="Rhea" id="RHEA:60376"/>
            <dbReference type="ChEBI" id="CHEBI:4167"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 18">Cell membrane</location>
            <topology evidence="4 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 18">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 18">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="18">Transport is mediated via a series of conformation changes, switching between a conformation where the substrate-binding cavity is accessible from the outside, and a another conformation where it is accessible from the cytoplasm.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 18">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014706461.1">
        <property type="nucleotide sequence ID" value="XM_014850975.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MI72"/>
    <dbReference type="Ensembl" id="ENSEAST00005025455.2">
        <property type="protein sequence ID" value="ENSEASP00005023459.2"/>
        <property type="gene ID" value="ENSEASG00005015964.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837436"/>
    <dbReference type="KEGG" id="eai:106837436"/>
    <dbReference type="CTD" id="6515"/>
    <dbReference type="GeneTree" id="ENSGT00940000160313"/>
    <dbReference type="OMA" id="GVFVYYM"/>
    <dbReference type="OrthoDB" id="4540492at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043204">
        <property type="term" value="C:perikaryon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005536">
        <property type="term" value="F:D-glucose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055056">
        <property type="term" value="F:D-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033300">
        <property type="term" value="F:dehydroascorbic acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046323">
        <property type="term" value="P:D-glucose import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17431">
        <property type="entry name" value="MFS_GLUT_Class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000040">
        <property type="entry name" value="Solute carrier family 2, facilitated glucose transporter member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002945">
        <property type="entry name" value="Glc_transpt_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045263">
        <property type="entry name" value="GLUT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23503:SF99">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 2, FACILITATED GLUCOSE TRANSPORTER MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01192">
        <property type="entry name" value="GLUCTRSPORT3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 18" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12 18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="9 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 17" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="154"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="270"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="305"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="333"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="361"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="426"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="19">
        <location>
            <begin position="13"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00034046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00039287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU368110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005023459.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="496" mass="54570" checksum="063791002E6113FE" modified="2023-09-13" version="2">MGTQKVTASLIFAISIATIGSFQFGYNTGVINAPEMIIRDFLNYSLEDKLTYRPTEVLLTSLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLIANLLAVTGGCLMGFCKLAESFEMLILGRLIIGLFCGLCTGFVPMYIGEISPTALRGAFGTLNQLGIVVGILVAQIFGLKVILGTDDLWPLLLAFTIIPAILQSAALPFCPESPRFLLINRKEEESTKKILQRLWGTQDVAQDIQEMKDESIRMAQEKQVTVLELFRAPNYRQPIIISIMLQLSQQLSGINAVFYYSTGIFRDAGVQEPIYATIGAGVVNTIFTVVSLFLVDRAGRRTLHMIGLGGMAFCSILMTVSLLLKGDYQWMSFVCIGAILIFVAFFEVGPGPIPWFIVAELFSQGPRPAAVAVAGCSNWTSNFLVGLLFPSAATYLGAYVFLVFSAFLVIFLVFTYFKVPETRGRTFEEITRAFEVQVQDPNRAEKGPMMEMNSIQPVKETTTNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MIJ5</accession>
    <name>A0A8C4MIJ5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Myotubularin-related protein 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Inactive phosphatidylinositol 3-phosphatase 10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MTMR10</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014700962.2">
        <property type="nucleotide sequence ID" value="XM_014845476.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MIJ5"/>
    <dbReference type="Ensembl" id="ENSEAST00005028308.2">
        <property type="protein sequence ID" value="ENSEASP00005026074.2"/>
        <property type="gene ID" value="ENSEASG00005017788.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106834051"/>
    <dbReference type="KEGG" id="eai:106834051"/>
    <dbReference type="CTD" id="54893"/>
    <dbReference type="GeneTree" id="ENSGT00940000156900"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13346">
        <property type="entry name" value="PH-GRAM_MTMR10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14593">
        <property type="entry name" value="PTP-MTMR10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000198">
        <property type="entry name" value="Myotubularin related protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036004">
        <property type="entry name" value="MTMR10_PH-GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030573">
        <property type="entry name" value="MTMR10_PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022587">
        <property type="entry name" value="MTMR12-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF39">
        <property type="entry name" value="MYOTUBULARIN-RELATED PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12578">
        <property type="entry name" value="3-PAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="7">
        <location>
            <begin position="214"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="331"/>
            <end position="332"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00069396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00079481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026074.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="768" mass="87642" checksum="0EDE2EDBF0FC6D26" modified="2023-09-13" version="1">MFSLKPPRPTFRSYLLPPPQTDDKINSEPKIKKLEPVLLPGEIVVNEVNFVRKCIATDTSQYDLWGKLVCSNFKISFITDDPMPLQKFHYRNLLLGEHDVPLTCIEQIVTVNDHKRKQKVLGPNQKLKFNPTELIIYCKDFRIVRFRFDESGPESAKKVCLAIAHYSQPTDLQLLFAFEYVGKKYHNSANKINGIPSGGGSGAAGGSSQKTPLFETYSDWDREIKRTGASGWRVCSVNEGYMISTCLPEYFVVPSSLADQDLKIFSHSFVGRRMPLWCWSHSNGSALVRMALIKDMLQQRKIEQRICNAVTKSHPQRSDVYKSDLDKTLPNIQEVQAAFVKLKQLCVNEPFEETEEKWLSSLENTRWLEYVRAFLKQSAELVYILERKHLSVVLQEEEGRDLSCIVASLVQVMLDPYFRTITGFQSLIQKEWVMAGYQFLDRCNHLKRSEKESPLFLLFLDATWQLLEQYPAAFEFSETYLAVLYDSTRISLFGTFLFNSPHQRVKQSTEFAISKNIQLGDEKGLRFPSVWDWSLQFTAKDRTLFHNPLYIGKSSPCVQNGSVKSFKRTKKSYSSTLRGMPASLKNGILSDQELLPRRNSLILKVKPEPPQQTDSQDSGVEQYFRECFSKPVDLHGVILPHLSGTHIKLWKLCYFRWVPEAQINLGGSITAFHRLSLLADEVDVLSRTLRQPHSGPLEACYAELDRSRLYFRASGPHDASGTPEFLSSSFPFSPVGNLCRRSILGTPLSKFLSGAKIWLSTETLANED</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MMM1</accession>
    <name>A0A8C4MMM1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Spermatosis associated serine rich 2 like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">SPATS2L</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPATS2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014687946.1">
        <property type="nucleotide sequence ID" value="XM_014832460.3"/>
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    <dbReference type="RefSeq" id="XP_014687947.1">
        <property type="nucleotide sequence ID" value="XM_014832461.3"/>
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        <property type="nucleotide sequence ID" value="XM_044768492.2"/>
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        <property type="nucleotide sequence ID" value="XM_044768493.2"/>
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        <property type="nucleotide sequence ID" value="XM_044768494.2"/>
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        <property type="nucleotide sequence ID" value="XM_044768495.2"/>
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        <property type="nucleotide sequence ID" value="XM_044768496.2"/>
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        <property type="nucleotide sequence ID" value="XM_070508319.1"/>
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        <property type="nucleotide sequence ID" value="XM_070508321.1"/>
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        <property type="nucleotide sequence ID" value="XM_070508322.1"/>
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        <property type="nucleotide sequence ID" value="XM_070508323.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A8C4MMM1"/>
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        <property type="protein sequence ID" value="ENSEASP00005029507.1"/>
        <property type="gene ID" value="ENSEASG00005020050.2"/>
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    <dbReference type="GeneID" id="106825582"/>
    <dbReference type="KEGG" id="eai:106825582"/>
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    <dbReference type="GeneTree" id="ENSGT00390000001138"/>
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        <property type="component" value="Chromosome 4"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="UBA-like_sf"/>
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        <property type="entry name" value="SPATS2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR15623">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED SERINE-RICH PROTEIN 2-RELATED"/>
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    <proteinExistence type="inferred from homology"/>
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            <begin position="63"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="382"/>
            <end position="524"/>
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    </feature>
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            <begin position="63"/>
            <end position="79"/>
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    </feature>
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            <begin position="80"/>
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            <begin position="407"/>
            <end position="418"/>
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            <begin position="440"/>
            <end position="456"/>
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        <location>
            <begin position="469"/>
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        <location>
            <begin position="514"/>
            <end position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007105"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MN45</accession>
    <name>A0A8C4MN45_EQUAS</name>
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        <submittedName>
            <fullName evidence="14">Zinc finger and BTB domain containing 45</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ZBTB45</name>
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    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_044760184.2"/>
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        <property type="nucleotide sequence ID" value="XM_044760185.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044616121.1">
        <property type="nucleotide sequence ID" value="XM_044760186.2"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_044760187.2"/>
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        <property type="nucleotide sequence ID" value="XM_070498921.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A8C4MN45"/>
    <dbReference type="Ensembl" id="ENSEAST00005032296.2">
        <property type="protein sequence ID" value="ENSEASP00005029714.2"/>
        <property type="gene ID" value="ENSEASG00005020198.2"/>
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    <dbReference type="GeneID" id="106846646"/>
    <dbReference type="CTD" id="84878"/>
    <dbReference type="GeneTree" id="ENSGT00940000160859"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18229">
        <property type="entry name" value="BTB_POZ_ZBTB45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000304">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000315">
        <property type="entry name" value="Zinc finger and BTB domain-containing protein 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050457">
        <property type="entry name" value="ZnFinger_BTB_dom_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105">
        <property type="entry name" value="AGAP004733-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46105:SF22">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN CONTAINING 45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BTB" evidence="12">
        <location>
            <begin position="33"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="401"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="429"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="457"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="484"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="184"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="296"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="341"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50157"/>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005029714.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="509" mass="53859" checksum="C03D4AE70B6E1848" modified="2023-09-13" version="2">MAAAEAVHHIHLQNFSRSLLETLNGQRLGGHFCDVTVRIREASLRAHRCVLAAGSPFFQDKLLLGHSEIRVPPVVPAQTVRQLVEFLYSGSLVVAQGEALQVLTAASVLRIQTVIDECTQIIARARAPAAGTPAPVPLPTPVPPPLAPAQLRHRLRHLLAARPPGHLGVAHSRKQRQPARLQLPAPPVPAKAEGSDADPTLPAVPDDGCDDDDEETDDETEGEEGEGGGPGEGQAPPSFPDCAAGFLSTARDSAREEPPAPAGLSDYGGAGRDFLRGASAAEDMYPDSYVSAWQDEDGTTAEGCPAETSAPPDCVLSGPRTPGVKTPGPPVALFPFHLGAPGPPVQPSPAPSGPAPAPPPTFYPPLQPDPAPSAPLGEAPAPPAAPSATPARAPGAEPPAYECSHCRKTFSSRKNYTKHMFIHSGEKPHQCAVCWRSFSLRDYLLKHMVTHTGVRAFQCAVCAKRFTQKSSLNVHMRTHRPERAPCPACGKVFSHRALLERHLAAHPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MR41</accession>
    <name>A0A8C4MR41_EQUAS</name>
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        <recommendedName>
            <fullName evidence="12">BAR/IMD domain-containing adapter protein 2-like 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Planar intestinal- and kidney-specific BAR domain protein</fullName>
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    </protein>
    <gene>
        <name evidence="19" type="primary">BAIAP2L2</name>
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    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Phosphoinositides-binding protein that induces the formation of planar or gently curved membrane structures. Binds to phosphoinositides, including to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) headgroups. There seems to be no clear preference for a specific phosphoinositide.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
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        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044635803.1">
        <property type="nucleotide sequence ID" value="XM_044779868.2"/>
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    <dbReference type="AlphaFoldDB" id="A0A8C4MR41"/>
    <dbReference type="Ensembl" id="ENSEAST00005029979.2">
        <property type="protein sequence ID" value="ENSEASP00005027603.1"/>
        <property type="gene ID" value="ENSEASG00005018784.2"/>
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    <dbReference type="GeneID" id="106837645"/>
    <dbReference type="KEGG" id="eai:106837645"/>
    <dbReference type="CTD" id="80115"/>
    <dbReference type="GeneTree" id="ENSGT00940000153560"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
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    <dbReference type="GO" id="GO:0044291">
        <property type="term" value="C:cell-cell contact zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0071439">
        <property type="term" value="C:clathrin complex"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:cytoplasmic vesicle membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:cytosol"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:nucleoplasm"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0051764">
        <property type="term" value="P:actin crosslink formation"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007009">
        <property type="term" value="P:plasma membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030838">
        <property type="term" value="P:positive regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07644">
        <property type="entry name" value="I-BAR_IMD_BAIAP2L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11914">
        <property type="entry name" value="SH3_BAIAP2L2"/>
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    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000056">
        <property type="entry name" value="brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 2"/>
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        <property type="entry name" value="brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 2"/>
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        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
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    <dbReference type="InterPro" id="IPR030126">
        <property type="entry name" value="Baiap2l2_I-BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013606">
        <property type="entry name" value="I-BAR_dom"/>
    </dbReference>
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        <property type="entry name" value="IRSp53/IRTKS/Pinkbar"/>
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    <dbReference type="InterPro" id="IPR035593">
        <property type="entry name" value="Pinkbar_SH3"/>
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    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001452">
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    <dbReference type="PANTHER" id="PTHR14206">
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    </dbReference>
    <dbReference type="Pfam" id="PF08397">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00326">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51338">
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    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="IMD" evidence="18">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="17">
        <location>
            <begin position="326"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="221"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="412"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="297"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="436"/>
            <end position="446"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054355"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00077036"/>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005027603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="512" mass="57250" checksum="17C44A7DA97C1197" modified="2022-01-19" version="1">MAPEMDQFYRSTVAIYKSIMEQFNPALENLVYLGNNYLRAFHALSEAAEVYFSAIQKIGEQALQSPTSQILGEILVQMSDTQRHLNSDLEVVVQTFHGDLLQHMEKNTKLDMQFIKDSRQHYEIEYRHRAANLEKCMSELWHMERKRDKNAREMKESVNRLHAQMQAFVSESQRAAELEEKRRYRFLAEKHLLLSNTFLQFFGRARGMLQNRVLLWKEQSEASRSPSRAHSPGLLGPVLGPPYPSGRLTPTRLDMPPRPLGEFGSPRSRHGSGSYGPEPTDARSASQLEPDRRSLPRTPSASSLYAGSAQPSRSNSFGERPGGGGGGARKVRALVSHSEGANHTLLRFSAGDVVEVLVPQAKNGWLYGKLEGSSTSGWFPEAYVKPLEEMPMNPLNPMNELPARSYPLRGSHSLDDLLDRPGNSTASSEHWDGQSRSRTPSRVPSRAPSPAPTPLPGSRRSSMGNMGVTSDVKKLTSWEQPPPELFPRGTNPFATVKLRPTITNDRSAPLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MRG6</accession>
    <name>A0A8C4MRG6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Antigen peptide transporter 2</fullName>
            <ecNumber evidence="18">7.4.2.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">ATP-binding cassette sub-family B member 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">TAP2</name>
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    <organism evidence="26 27">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
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                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
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                <person name="S."/>
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                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
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                <person name="G."/>
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                <person name="M."/>
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                <person name="J."/>
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                <person name="X. and Zhong"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <reaction evidence="19">
            <text>a peptide antigen(in) + ATP + H2O = a peptide antigen(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:65972"/>
            <dbReference type="Rhea" id="RHEA-COMP:16941"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:166823"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.4.2.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:65973"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily.</text>
    </comment>
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    <dbReference type="AlphaFoldDB" id="A0A8C4MRG6"/>
    <dbReference type="Ensembl" id="ENSEAST00005033120.2">
        <property type="protein sequence ID" value="ENSEASP00005030464.2"/>
        <property type="gene ID" value="ENSEASG00005020743.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160499"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042825">
        <property type="term" value="C:TAP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015421">
        <property type="term" value="F:ABC-type oligopeptide transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015433">
        <property type="term" value="F:ABC-type peptide antigen transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0023029">
        <property type="term" value="F:MHC class Ib protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042605">
        <property type="term" value="F:peptide antigen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046978">
        <property type="term" value="F:TAP1 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002485">
        <property type="term" value="P:antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0002481">
        <property type="term" value="P:antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0046967">
        <property type="term" value="P:cytosol to endoplasmic reticulum transport"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0002502">
        <property type="term" value="P:peptide antigen assembly with MHC class I protein complex"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:peptide antigen transport"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0001916">
        <property type="term" value="P:positive regulation of T cell mediated cytotoxicity"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:T cell mediated cytotoxicity"/>
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    <dbReference type="CDD" id="cd18590">
        <property type="entry name" value="ABC_6TM_TAP2"/>
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        <property type="entry name" value="Antigen peptide transporter 2"/>
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    <dbReference type="FunFam" id="3.40.50.300:FF:000140">
        <property type="entry name" value="Lipid A export ATP-binding/permease protein MsbA"/>
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    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
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    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
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    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
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    <dbReference type="InterPro" id="IPR013305">
        <property type="entry name" value="ABC_Tap-like"/>
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    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
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    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR005293">
        <property type="entry name" value="Tap2/ABCB3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00958">
        <property type="entry name" value="3a01208"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43394">
        <property type="entry name" value="ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TRANSPORTER 2, ATP BINDING CASSETTE SUBFAMILY B"/>
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        <property type="entry name" value="ABC_tran"/>
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        <property type="entry name" value="TAP2PROTEIN"/>
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    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
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    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0391">Immunity</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
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    <keyword evidence="5 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
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    <feature type="chain" id="PRO_5040434464" description="Antigen peptide transporter 2" evidence="23">
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            <begin position="382"/>
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    <feature type="domain" description="ABC transmembrane type-1" evidence="25">
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            <begin position="153"/>
            <end position="435"/>
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    </feature>
    <feature type="domain" description="ABC transporter" evidence="24">
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            <end position="680"/>
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        <source>
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    <evidence type="ECO:0000259" key="24">
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<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="9">F-box only protein 32</fullName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="7">Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
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    <comment type="subunit">
        <text evidence="8">Part of the SCF (SKP1-CUL1-F-box) E3 ubiquitin-protein ligase complex SCF(FBXO32) formed of CUL1, SKP1, RBX1 and FBXO32.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        <property type="entry name" value="F-BOX ONLY PROTEIN 32"/>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Yin"/>
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                <person name="C."/>
                <person name="Jin"/>
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                <person name="Yue"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="Amills"/>
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                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="nucleotide sequence ID" value="XM_044768599.2"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="ZnF-G_patch_domain"/>
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    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17614:SF13">
        <property type="entry name" value="ZINC FINGER PROTEIN 804A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17614">
        <property type="entry name" value="ZINC FINGER-CONTAINING"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="5">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="179"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="591"/>
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    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="764"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="179"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="410"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="593"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
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        </location>
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        <source>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
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        </source>
    </evidence>
    <sequence length="1208" mass="136474" checksum="878A5533A03DC20D" modified="2023-09-13" version="2">MECYYIVISSTHLSNGHFRNIKGVFRGPLSKNGDKTLDYAEKENTIAKALEDLKANFYCELCDKQYYKHQEFDNHINSYDHAHKQRLKELKQREFARNVASKSRKDERKQEKALQRLHKLAELRKETVCAPGSGPMFKSTTVTVRENCNEISRRVVVDSVNNQEDFKYTLIHSEESAKDVTTVTTDPESAGNYTAKNNQRGDQAQGLHRHKIGFSFAFPKKASVKLESSAAAFSEYNDDASVEKGFSRKSRFVPGACHLQLSSPTDVLLGSEEKANSFHIPEGMCTDKETAQTQEMKEVSSEQDALLLPSFYQFQLPLSSDADSCQNSVPLADQILQEDVIINEDISMSDNSSELLGNKSTVLDMANDCISLQATTEENVKDNDVSVIEVENKNHGPEMLAPSNSEEDNTTLHKKTDPYKRPSEPFVPVLNKDRSTVLQWPSEMLTYTTTQPSISYSCNPLCFDFKSTKLNNNLDKNKLPLNDLYSQQNGEDICKRPVLDCKDTSTAELTDYEIGGSRNEYTQVTPLLADDNTLSNTCDSGKNENTDQRYKNISCRIRKTKKYNLTKNQIKQDTVDEKYNKMRLKDTHEHWFHKSRRKKKRRRLCQHHSGEKTKESETHFKMEMENSYTDTTRKNLLETITEKEYLAAEQLLDSYQLPDKRAKSASTNLSANEEICKTWSSEYNNDDAVSSKTHCKKNSIVLNGKSSPTMIHPGKHNLTYSRTYCNWKAKMSSCSQDHRCLVLPNDMKCLSQNQAVKRGYNSLINESERSHRKRRQHSHSYSSDESLNRQNYLPEEFLRPPRASAPCKPKRKRRRKRSRFHTGFEALELKENTDYPMKGNSSLHYQGELISEDTKEEIKPQEIANVERNLEQTDQVENKLHPSSPLPSEISGETEPLVMDTTPGELPEISNEPTTSVYVASAPTKEEINNTLLKHKERSENRDVNEKQIPCKVPNIERNSRQSRPKSYLCHYELAEVPPQGKMNEASTEWLRFNSGILNAQPPLPFKEAHVSGHTFVTTEQILAPLALPEQALLIPIENHDKFKNLPCEVYQHIIPANMLANKVKFTFPPAALPPPSTPLQPLPLQQPLCSTSVTTIHHTVLQQHAAAAAAAAAAAAAGTFKVLQPHQQFLSQVPALTRTSLPQISVGPVGPRLCPGNQPTFVAPPQMPVIPASVLHPGHLAFPPLPHALFPSLLSPHPTVIPLQPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N6R1</accession>
    <name>A0A8C4N6R1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Beta/alpha-defensin C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4N6R1"/>
    <dbReference type="Ensembl" id="ENSEAST00005035651.2">
        <property type="protein sequence ID" value="ENSEASP00005032714.2"/>
        <property type="gene ID" value="ENSEASG00005022353.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01090000260492"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031731">
        <property type="term" value="F:CCR6 chemokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042056">
        <property type="term" value="F:chemoattractant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060326">
        <property type="term" value="P:cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.360.10:FF:000001">
        <property type="entry name" value="Beta-defensin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.360.10">
        <property type="entry name" value="Antimicrobial Peptide, Beta-defensin 2, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006080">
        <property type="entry name" value="Beta/alpha-defensin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001855">
        <property type="entry name" value="Defensin_beta-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20515">
        <property type="entry name" value="BETA-DEFENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20515:SF2">
        <property type="entry name" value="DEFENSIN BETA 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00711">
        <property type="entry name" value="Defensin_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00048">
        <property type="entry name" value="DEFSN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57392">
        <property type="entry name" value="Defensin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0044">Antibiotic</keyword>
    <keyword evidence="3" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="4" id="KW-0211">Defensin</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Beta/alpha-defensin C-terminal" evidence="7">
        <location>
            <begin position="25"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005032714.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="97" mass="10451" checksum="9A48CA0A5575E3AF" modified="2023-09-13" version="2">WAAQHSVLVLETRGFTAGIGNPISCARNRGVCIPIGCLPGMKQIGTCGLPGTKCCRKNQKLLPLQNMQNLNHIKTLRKQRILPPGHCGCCVVFDQAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PF69</accession>
    <name>A0A8C4PF69_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein Atg16l2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">APG16-like 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Autophagy-related protein 16-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ATG16L2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">May play a role in regulating epithelial homeostasis in an ATG16L1-dependent manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat ATG16 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PF69"/>
    <dbReference type="Ensembl" id="ENSEAST00005000534.2">
        <property type="protein sequence ID" value="ENSEASP00005000442.2"/>
        <property type="gene ID" value="ENSEASG00005000424.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153936"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034274">
        <property type="term" value="C:Atg12-Atg5-Atg16 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0039689">
        <property type="term" value="P:negative stranded viral RNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000199">
        <property type="entry name" value="autophagy-related protein 16-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045160">
        <property type="entry name" value="ATG16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013923">
        <property type="entry name" value="Autophagy-rel_prot_16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19878">
        <property type="entry name" value="AUTOPHAGY PROTEIN 16-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19878:SF7">
        <property type="entry name" value="PROTEIN ATG16L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08614">
        <property type="entry name" value="ATG16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5 13" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Autophagy-related protein 16" evidence="16">
        <location>
            <begin position="17"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="331"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="375"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="417"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="555"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="59"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="117"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00076331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00082487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000442.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="618" mass="68561" checksum="AC799635A0B64849" modified="2023-09-13" version="2">MAGPGAPAGLAARWKRHIVRQLRQRDRTQKALFLELVPAYNRLLEKAELLARFSEKIQPEPNDVTPASHQGLWEEESGPDSDQVPGTLRVKWQEEEEGLRLVCGEMAYQVVEKSAALGALEAELEQRQSRLAELEARVAQLQEERTQQAQRVHVRRARNAAQRAAYEALRAHAGVQEAALRRLEEEARELLERLVQRKARAAAERNLRNERRERAKQARVSQELKKAAKRTVSISESPSILGYAVREPAKTLALAAEPESLESEAGEKWRRPFRSASATSLTLSRCVDVVKGLLDFKKRRGHSVGGAPEQRYQSIPVCVAARLPTWTQDVLDAHLSEVNAVCFGPNSSLLATGGADSLIHLWNVVGGHLKAHQTLEGGGGSITSVDFDPSGSQVLAATYNRAAQLWKVGEAQSKETLSGHTDKVTAAKFKLTRHEAVTGSRDRTVKEWDLGRAYCSRTINVLSYCNDVVCGDHLIISGHNDQKIRFWDSRGPRCTQVIPVQGRVTSLSLSHDQLHLLSCSRDDTLKVIDLRVSNIRQVFRAEGFKCGSDWTKAVFSPDRCYALAGSWDGALYIWDVDTGKLESSLRGPHCAAVNAVAWCYSGTHVVSVDQARKVVLWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PG88</accession>
    <name>A0A8C4PG88_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Palmitoyltransferase</fullName>
            <ecNumber evidence="14">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ZDHHC5</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:36684"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="14"/>
    <dbReference type="RefSeq" id="XP_014683287.1">
        <property type="nucleotide sequence ID" value="XM_014827801.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014683288.1">
        <property type="nucleotide sequence ID" value="XM_014827802.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PG88"/>
    <dbReference type="Ensembl" id="ENSEAST00005002957.2">
        <property type="protein sequence ID" value="ENSEASP00005002694.2"/>
        <property type="gene ID" value="ENSEASG00005002005.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822556"/>
    <dbReference type="KEGG" id="eai:106822556"/>
    <dbReference type="CTD" id="25921"/>
    <dbReference type="GeneTree" id="ENSGT00940000156001"/>
    <dbReference type="OrthoDB" id="4096362at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045335">
        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019706">
        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015908">
        <property type="term" value="P:fatty acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044546">
        <property type="term" value="P:NLRP3 inflammasome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160075">
        <property type="term" value="P:non-canonical inflammasome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070427">
        <property type="term" value="P:nucleotide-binding oligomerization domain containing 1 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070431">
        <property type="term" value="P:nucleotide-binding oligomerization domain containing 2 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900227">
        <property type="term" value="P:positive regulation of NLRP3 inflammasome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905171">
        <property type="term" value="P:positive regulation of protein localization to phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903078">
        <property type="term" value="P:positive regulation of protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140639">
        <property type="term" value="P:positive regulation of pyroptotic inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070534">
        <property type="term" value="P:protein K63-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141201">
        <property type="term" value="P:pyroptotic cell death"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032495">
        <property type="term" value="P:response to muramyl dipeptide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001594">
        <property type="entry name" value="Palmitoyltrfase_DHHC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12349">
        <property type="entry name" value="ANKYRIN REPEAT AND LEM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12349:SF3">
        <property type="entry name" value="PALMITOYLTRANSFERASE ZDHHC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01529">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50216">
        <property type="entry name" value="DHHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="16">
        <location>
            <begin position="102"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="289"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="359"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="422"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="442"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="668"/>
            <end position="677"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023463"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU079119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005002694.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="715" mass="77472" checksum="3C5A47B1591C7FAE" modified="2023-09-13" version="2">MPAESGKRFKPSKYVPVSAAAIFLVGATTLFFAFTCPGLSLYVSPAVPIYNAIVFLFVLANFSMATFMDPGIFPRAEEDEDKEDDFRAPLYKTVEIKGIQVRMKWCATCRFYRPPRCSHCSVCDNCVEEFDHHCPWVNNCIGRRNYRYFFLFLLSLTAHIMGVFGFGLLYVLYHMEELSGVRTAVTMAVMCVAGLFFIPVAGLTGFHVVLVARGRTTNEQVTGKFRGGVNPFTNGCCNNVSRVLCSSPAPRYLGRPKKEKTIVIRPPFLRPDVSDGQITVKIMDNGIQGELRRSKSKGSLEITESQSADAEPPPPPKPDLSRYTGLRTHLTLATNEDTSLLGKDSPPTPTMYKYRPGYSSSSTSAAMPHSSSAKLSRGDSLKEPTSIAESSRHPSYRSEPSLEPESFRSPTFGKSFHFDPLSSGSRSSSLKSAQGTGFELGQLQSIRSEGTTSTSYKSLANQTRNGSLSYDSLLTPSDSPDFESVQAGPEPDPPLGYTSPFLSARLAQQREAERHPRLVPTGPTHREPSPVRYDNLSRHIVASLQEREKLLRQSPPLPGREEEPGLGDSGIQSTPGSGHAPRTSSSSDDSKRSPLGKTPLGRPAAPRFGKPDGLRGRGIGSPEPGPTAPYLGRSMSYSSQKAPPGVSETEEVALQPLLTPKDEVQLKTAYSKSNGQPKSIGSASPGPGQPPLSSPTRGGVKKVSGVGGTTYEISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PSL3</accession>
    <name>A0A8C4PSL3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Metalloendopeptidase</fullName>
            <ecNumber evidence="20">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">BMP1</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="19 20">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="19 20">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4PSL3"/>
    <dbReference type="Ensembl" id="ENSEAST00005030047.2">
        <property type="protein sequence ID" value="ENSEASP00005027670.2"/>
        <property type="gene ID" value="ENSEASG00005018828.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157176"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061036">
        <property type="term" value="P:positive regulation of cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04281">
        <property type="entry name" value="ZnMc_BMP1_TLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000022">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000004">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000007">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000009">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000011">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000014">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000004">
        <property type="entry name" value="Metalloendopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015446">
        <property type="entry name" value="BMP_1/tolloid-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001506">
        <property type="entry name" value="Peptidase_M12A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006026">
        <property type="entry name" value="Peptidase_Metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034036">
        <property type="entry name" value="ZnMP_TLD/BMP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF53">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01400">
        <property type="entry name" value="Astacin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14670">
        <property type="entry name" value="FXa_inhibition"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001199">
        <property type="entry name" value="BMP_1/tolloid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00480">
        <property type="entry name" value="ASTACIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00235">
        <property type="entry name" value="ZnMc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51864">
        <property type="entry name" value="ASTACIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="14 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 18" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 19 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 17 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 19 20" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5 19 20" id="KW-0645">Protease</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 20" id="KW-0732">Signal</keyword>
    <keyword evidence="10 17 19" id="KW-0862">Zinc</keyword>
    <keyword evidence="13" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040528167" description="Metalloendopeptidase" evidence="20">
        <location>
            <begin position="27"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12A" evidence="24">
        <location>
            <begin position="125"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="22">
        <location>
            <begin position="326"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="22">
        <location>
            <begin position="439"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="23">
        <location>
            <begin position="551"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="22">
        <location>
            <begin position="595"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="22">
        <location>
            <begin position="751"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="22">
        <location>
            <begin position="864"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="94"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="94"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="110"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="active site" evidence="16 19">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17 19">
        <location>
            <position position="217"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17 19">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17 19">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="189"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001199-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR001199-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU361183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51864"/>
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    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005027670.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="990" mass="111930" checksum="C9849E197185A8DD" modified="2023-09-13" version="2">MPGVARLPLPLPLLLWLLLLARPGRPLDLADYTYELGEEDDSEPLNYKDPCKAAAFLGDIALDEEDLRAFQVQQAVDLGQQAIRRSSIKAAVSENSSTPICQSTSGQPQRKNRGRWRSRSRSRRAATSRPERVWPDGVIPFVIGGNFTGSQRAVFRQAMRHWEKHTCVTFLERTDEDSYIVFTYRPCGCCSYVGRRGGGPQAISIGKNCDKFGIVVHELGHVIGFWHEHTRPDRDRHVSIVRENIQPGQEYNFLKMELQEVESLGENYDFDSIMHYARNTFSRGIFLDTIVPKYEVNGVKPPIGQRTRLSKGDIAQARKLYKCPACGETLQDSTGNFSSPEYPNGYSAHMHCVWRISVTPGEKIILNFTSMDLYRSRLCWYDYVEVRDGFWRKAPLRGRFCGAKLPEPVVSTDSRLWVEFRSSSNWVGKGFFAVYEAICGGDVKKDNGHIQSPNYPDDYRPSKVCVWRIQVSEGFHVGLTFQSFEIERHDSCAYDYLEVRDGHSEGSPLIGRYCGYERPDDIKSTSSRLWLKFVSDGSINKAGFAVNFFKEVDECSRPNRGGCEQRCLNTLGSYKCSCDPGYELAPDKRRCEAACGGFLTKLNGSITSPGWPKEYPPNKNCIWQLVAPTQYRISLQFDFFETEGNDVCKYDFVEVRSGLTADSKLHGKFCGSEKPEVITSQYNNMRVEFKSDNTVSKKGFKAHFFSDKDECSKDNGGCQQDCVNTFGSYECQCRSGFILHDNKHDCKEAGCDHKVTSTSGTITSPNWPDKYPSKKECTWAISSTPGHRVKLTFTEMDIESQPECAYDHLEVYDGRDAKADILGRFCGSKKPEPILATGSRMFLRFYSDNSVQRKGFQASHSTECGGQVQAEVKTKDLYSHAQFGDNNYPGGVDCEWVIVAEEGYGVELVFQTFEVEEETDCGYDYMELFDGYDSTAPRLGRYCGSGPPEEVYSAGDSVLVKFHSDDTITKKGFHLRYTSTKFQDTLHSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PVH7</accession>
    <name>A0A8C4PVH7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Death-inducer obliterator 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Death-associated transcription factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">LOC106836328</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="14">Interacts specifically (via PHD-type zinc finger) with histone H3 that is trimethylated at 'Lys-4' (H3K4me3), histone phosphorylation at 'Thr-3' or 'Thr-6' disrupts this binding and promotes translocation of DIDO1 from chromatin to the mitotic spindle during mitosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005036973.2">
        <property type="protein sequence ID" value="ENSEASP00005033919.2"/>
        <property type="gene ID" value="ENSEASG00005023191.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155532"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097190">
        <property type="term" value="P:apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15639">
        <property type="entry name" value="PHD_DIDO1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.30:FF:000002">
        <property type="entry name" value="Death-inducer obliterator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000225">
        <property type="entry name" value="Death-inducer obliterator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.30">
        <property type="entry name" value="Transcription elongation factor S-II, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033082">
        <property type="entry name" value="DIDO1_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012921">
        <property type="entry name" value="SPOC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003618">
        <property type="entry name" value="TFIIS_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036575">
        <property type="entry name" value="TFIIS_cen_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477:SF13">
        <property type="entry name" value="DEATH-INDUCER OBLITERATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477">
        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07744">
        <property type="entry name" value="SPOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07500">
        <property type="entry name" value="TFIIS_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00510">
        <property type="entry name" value="TFS2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51321">
        <property type="entry name" value="TFIIS_CENTRAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="19">
        <location>
            <begin position="268"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="domain" description="TFIIS central" evidence="20">
        <location>
            <begin position="671"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="545"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="647"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="775"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="860"/>
            <end position="960"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="48"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="116"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="146"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="545"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="557"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="658"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
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            <begin position="801"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="879"/>
            <end position="888"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="919"/>
            <end position="929"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00065323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00083537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005033919.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1288" mass="139950" checksum="C339395823C7DF81" modified="2023-09-13" version="2">MDEKGDPSNEDAPKAIKPTSKEFRKTWGFRRTTIAKREGAGDAELDSLEQQPRQQQQSLSLRRSGRQPKRTERVEEFLTTVRRRGRKNVPVSLEDSSEPTSCPVTDVETASEGSVESTSEMKSGSKSGSRGVKERPASSAKAKGGDDEDDTSDSDSDGLTLKELQNRLRRKREQEPANRPLKGIQNRLRKKRQEEDPAEIVDVEAGDAAGSSLSGTQEPEAAQGPVSQGAKDDRESQLEGKAAQGMKDEEPGASVRHKPECEVYDPNALYCMCRQPHNNRFMICCDRCEEWFHGDCVGISEARGRLLERNGEDYICPNCTILQVQDETSTETAGQQDTRVRPADADGTDFTSIGTVEQKCSEDQGIKGRIEKAANPSGKKKLKIFQPVVEAPGASKCIGPGCSSVAQPDSVYCSNDCILKHAAATMKFLNSGKEQKPKPKEKTKTKPEKLILPKCSVQAGVKISSVHKRPAPDKKENTVKKAMVAPSRSEVLIKETTCESSTPSWASDHNYNAVKPEQTAAPWPSLLYKSMKEDRRLEEHTAMAAAAPKKTAPPGSSVGSRQPSSRNLVPKKPPHFANMVAAKSAIKKSPSGFKGTIPKRPWLPAASGVSAAKQAGLAPVTTTAASKKLPGSAALVGAIRKPVASSAPLASTAPGRLGTSSPAPSQPNSQIRQNIRRSLKEILWKRVSDSDDLIMTENEVGKIALHIEKEMFNLFRVTDNRYKSKYRSIMFNLKDPKNQGLFHRVLREEISLAKLVRMKPEELVSKELSVWKERPTKPVMEPRPKLHNESKKTAAKQEVVPDMEDSPPVSDSDEQQELVRAAPEKSTAPLLDVFSSMLKDTTSQHRAHLFDLNCKICTGQVPSSEDEPAPKKQKLSASAKKEESKSKCDASPSDLVLSSADEVMPDTLPENASEPDLESASHAHLERKYCPVPPGDGHPEPSTLEGHSSCPASSGGGVLTTVTVSGRDPRTAPSGSCTVAVPTAAHLDSTHPVEPRQDVLKPAATSVTVPKSILAKPSSSPEPRYLLSVPPSPGISISESRSPPEGDTTLFLSRLSTIWKGFINMQSVAKFVTKAYPVSGCFDYLSEDLPDTIHIGGRIAPRTVWDYVGKLKSSVSKELCLIRFHPATEEEEVAYISLYSYFSSRGRFGVVANNSRHVKDLYLIPLSAKDPVPSKLLPFEGPALFQRTAGKPESRVQLTVFSTLDVSPARWCLHGWGFTAGDSGSDGLHVLGPPYSWKEDQSDWTLIFCASLCDPNGTGPRPGHPSLPSQSVGLGFSVQKACGVTVGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I5M5</accession>
    <name>A0A9L0I5M5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Archaemetzincin-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Archeobacterial metalloproteinase-like protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">AMZ2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Probable zinc metalloprotease.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M54 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014703140.1">
        <property type="nucleotide sequence ID" value="XM_014847654.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014703141.1">
        <property type="nucleotide sequence ID" value="XM_014847655.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014703143.1">
        <property type="nucleotide sequence ID" value="XM_014847657.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044601553.1">
        <property type="nucleotide sequence ID" value="XM_044745618.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044601554.1">
        <property type="nucleotide sequence ID" value="XM_044745619.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044601555.1">
        <property type="nucleotide sequence ID" value="XM_044745620.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044601556.1">
        <property type="nucleotide sequence ID" value="XM_044745621.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044601557.1">
        <property type="nucleotide sequence ID" value="XM_044745622.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044601559.1">
        <property type="nucleotide sequence ID" value="XM_044745624.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044601560.1">
        <property type="nucleotide sequence ID" value="XM_044745625.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070339593.1">
        <property type="nucleotide sequence ID" value="XM_070483492.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070339594.1">
        <property type="nucleotide sequence ID" value="XM_070483493.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070339595.1">
        <property type="nucleotide sequence ID" value="XM_070483494.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070339596.1">
        <property type="nucleotide sequence ID" value="XM_070483495.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070339597.1">
        <property type="nucleotide sequence ID" value="XM_070483496.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070339599.1">
        <property type="nucleotide sequence ID" value="XM_070483498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0I5M5"/>
    <dbReference type="SMR" id="A0A9L0I5M5"/>
    <dbReference type="Ensembl" id="ENSEAST00005078204.1">
        <property type="protein sequence ID" value="ENSEASP00005035439.1"/>
        <property type="gene ID" value="ENSEASG00005016006.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835354"/>
    <dbReference type="KEGG" id="eai:106835354"/>
    <dbReference type="CTD" id="51321"/>
    <dbReference type="GeneTree" id="ENSGT00530000063996"/>
    <dbReference type="OMA" id="CQWLSCV"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11375">
        <property type="entry name" value="Peptidase_M54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052009">
        <property type="entry name" value="Archaemetzincin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012962">
        <property type="entry name" value="Pept_M54_archaemetzincn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32205:SF5">
        <property type="entry name" value="ARCHAEMETZINCIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32205">
        <property type="entry name" value="ARCHAEMETZINCIN-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07998">
        <property type="entry name" value="Peptidase_M54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005035439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="368" mass="42145" checksum="18B7C43EA1FF63ED" modified="2023-09-13" version="1">MQTVRHSEQTLKTALISKNPALVSQYEKLDAGERRLMNQAFQPNSDLFGPITVHSRSDWITSHPEDPQDFEQFFSDPYRKTPSPEKRSIYIQCIGSLGNTRAISEEYIKWLQGYCEAFFYGLTVKLLAPVPVSATRCSFRVNDSTQNLQIHAGHILKFLKKKKPGDAFCIVGVTMIDLYPRDSWNFVFGQASLTDGVGIFSFARYGSDFYSSRYEGRVKKPQKVSSSDYSVFDNYYIPEVTSVLLLRSCKTLTHEIGHIFGLRHCQWLACLMQGSNHLEEADRRPLNLCPICLRKLQCAIGFNIVERYKALVRWIDGASADTPRVTPKHSREDDVNLPKPVEAFKEWKEWIIKCLAVLRKEEPSNRSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I6C7</accession>
    <name>A0A9L0I6C7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Ribosomal protein S6 kinase delta-1</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">52 kDa ribosomal protein S6 kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">RPS6KC1</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">May be involved in transmitting sphingosine-1 phosphate (SPP)-mediated signaling into the cell. Plays a role in the recruitment of PRDX3 to early endosomes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Interacts with SPHK1 and phosphatidylinositol 3-phosphate. Interacts (via PX domain) with PRDX3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_014690331.1">
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        <property type="protein sequence ID" value="ENSEASP00005035221.1"/>
        <property type="gene ID" value="ENSEASG00005002866.2"/>
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        <property type="component" value="Chromosome 25"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:lysosome"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02677">
        <property type="entry name" value="MIT_SNX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07287">
        <property type="entry name" value="PX_RPK118_like"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd05576">
        <property type="entry name" value="STKc_RPK118_like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ribosomal protein S6 kinase delta-1 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ribosomal protein S6 kinase delta-1 isoform X1"/>
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        <property type="entry name" value="ribosomal protein S6 kinase delta-1 isoform X1"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.80">
        <property type="entry name" value="Phosphotransferase system, lactose/cellobiose-type IIA subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051866">
        <property type="entry name" value="Intracell_Sig-Traffick_Protein"/>
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    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007330">
        <property type="entry name" value="MIT_dom"/>
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        <property type="entry name" value="MIT_dom_sf"/>
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        <property type="entry name" value="Prot_kinase_dom"/>
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        <property type="entry name" value="PX_dom"/>
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        <property type="entry name" value="PX_S6K-delta-1"/>
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        <property type="entry name" value="STK_RPK118-like"/>
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        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15508:SF2">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 KINASE DELTA-1"/>
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        <property type="entry name" value="Pkinase"/>
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        <property type="entry name" value="S_TKc"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Protein kinase-like (PK-like)"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PX" evidence="24">
        <location>
            <begin position="8"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="23">
        <location>
            <begin position="764"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="201"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="440"/>
            <end position="509"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="212"/>
            <end position="223"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="440"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="22">
        <location>
            <begin position="493"/>
            <end position="504"/>
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            <dbReference type="ARBA" id="ARBA00004370"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
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            <dbReference type="ARBA" id="ARBA00012513"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50011"/>
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    </evidence>
    <evidence type="ECO:0000259" key="24">
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            <dbReference type="PROSITE" id="PS50195"/>
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    <evidence type="ECO:0000313" key="25">
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    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1067" mass="118540" checksum="218DE0BF46B261B3" modified="2023-09-13" version="1">MASPRERGADLARFYTVTDPQRHPRGYTVYKVTARVVSRRNPEDVQEIIVWKRYSDFKKLHKELWQIHKNLFRHSELFPPFAKGIVFGRFDETVIEERRQCAEDLLQFSANIPALYNSKQLEDFFKGGIINDGSELIGPAEAYSDSLTDNFPECSTEGFSSDSDLVSLTVDVDSLAELDDGMASNQNSPIRTFGLNQSSDSSALGAVASDSEQNKAEEERESRSLFPGSLKSKLGKRDYLEKAGELIKLALKKEEEDDYEAASDFYRKGVDLLLEGVQGESSPTRREAVKRRTAEYLMRAESLCSLYKGKPQLDDVAQPPGSLSSRPPWNLRSPAEELKAFRVLGVIDKVLLVVDTRTEQTFILKGLRKSSEYSRNRKTIIPRCVPNMVCLHKYIVSEESVFLVLQHAEGGKLWSYISKFLNRSLEESFDIEEVKKSTLSKVHLQQPTSGPRDSSSFESRGSDGGTMLKVLPLKTSLTPSSQEDSYQDDDGQDSSPKWPDSGSSSEEECTTSYLTLCNEYGQEKIEPGSLSEEPFMKTEGIGVDTKAIESFPACLAPDSDSPSTQLIAHELKFFPGDDGLEAVSSPGTSDSLSRSKNSPMEFFRIDSKDSTSELLGLDFGEKLYSRKSEPLKPFFTLPDGDSTSRSFSISESKVELIAQDTISRGSDDSVPVISFKDAAFDDLSGTDEGRPDLLVNLPGELEPPKDGAAMGPTKFVQTNIGIMESKLLEAPDVLCLRLSTEQCQAYEEKGTEELSDPSRPKSHSLTEKHYAQGELGMLFAAAVDHCSSGDRSLLHSSDPKFQGLGVVASAVTANSTEESLFHISNPLSGANEYNANTETLKTEEVLLFTDQTDDLAKEEPTLFQRDYETRGDSGLALQGDKEIHHIFEDLDKKLALNSRFYIPEGCIQRWAAEMVVALDALHREGIVCRDLNPNNILLNDRGHIQLTYFSRWSEVEDSCDSDAIERMYCAPEVGAITEETEACDWWSLGAVLFELLTGKTLVECHPAGINTHTTLNMPECVSEEARSLIQQLLQFNPVERLGAGVAGVEDIKSHPFFTLVDWAELMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I6H4</accession>
    <name>A0A9L0I6H4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase</fullName>
            <ecNumber evidence="4">3.5.1.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Phosphatidylinositol-glycan biosynthesis class L protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PIGL</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the second step of glycosylphosphatidylinositol (GPI) biosynthesis, which is the de-N-acetylation of N-acetylglucosaminyl-phosphatidylinositol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 6-(N-acetyl-alpha-D-glucosaminyl)-1-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol + H2O = a 6-(alpha-D-glucosaminyl)-1-(1,2-diacyl-sn-glycero-3-phospho)-1D-myo-inositol + acetate</text>
            <dbReference type="Rhea" id="RHEA:11660"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57265"/>
            <dbReference type="ChEBI" id="CHEBI:57997"/>
            <dbReference type="EC" id="3.5.1.89"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:11661"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGL family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.89" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0I6H4"/>
    <dbReference type="Ensembl" id="ENSEAST00005077344.1">
        <property type="protein sequence ID" value="ENSEASP00005035872.1"/>
        <property type="gene ID" value="ENSEASG00005024611.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106838863"/>
    <dbReference type="KEGG" id="eai:106838863"/>
    <dbReference type="CTD" id="9487"/>
    <dbReference type="GeneTree" id="ENSGT00390000018434"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000225">
        <property type="term" value="F:N-acetylglucosaminylphosphatidylinositol deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10320:FF:000002">
        <property type="entry name" value="Probable N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
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    <keyword evidence="5" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
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<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I6J1</accession>
    <name>A0A9L0I6J1_EQUAS</name>
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        <recommendedName>
            <fullName evidence="9 10">Apoptotic protease-activating factor 1</fullName>
            <shortName evidence="10">APAF-1</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">APAF1</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="10">Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP.</text>
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    <comment type="subunit">
        <text evidence="10">Monomer. Oligomerizes upon binding of cytochrome c and dATP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:apoptosome"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="term" value="F:ADP binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0008656">
        <property type="term" value="F:cysteine-type endopeptidase activator activity involved in apoptotic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030900">
        <property type="term" value="P:forebrain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070059">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001843">
        <property type="term" value="P:neural tube closure"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051402">
        <property type="term" value="P:neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of apoptotic signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:regulation of apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd08323">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD40_repeat_CS"/>
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    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
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    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048975">
        <property type="entry name" value="WHD_APAF1"/>
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    <dbReference type="PANTHER" id="PTHR22845">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR22845:SF5">
        <property type="entry name" value="APOPTOTIC PROTEASE-ACTIVATING FACTOR 1"/>
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        <property type="entry name" value="WD_REPEATS_REGION"/>
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    <keyword evidence="4 10" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="8 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 12" id="KW-0853">WD repeat</keyword>
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            <begin position="1"/>
            <end position="90"/>
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        <location>
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    </feature>
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    </feature>
    <feature type="repeat" description="WD" evidence="12">
        <location>
            <begin position="1130"/>
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        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="154"/>
            <end position="161"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR037646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR037646-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005035894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1256" mass="142519" checksum="6BD51E0353169632" modified="2023-09-13" version="1">MDAKARNCLLQHREALEKDIKTSYIMDYMIGDGVLTVSEEEKVKNEPTRQQKAAMLIKMILKKDNYCYISFYKALLQEGYKDLAALLHGGIPVISSSNGKDSLVGVTSYVKTVLCEGGVPQRPVVFVTRKKLVHTIRQKLLKLDGEPGWVTIYGMAGCGKSVLAAEAVRDHTLLEGCFPGGVHWLSVGKQDKSGLLMKLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWDPWVLKAFDNQCQILLTTRDRSVTDSVMGPKYVIPVESGLGKEKGLEILSLFVNMKKADLPEQAHSIIKECKGSPLVVSLIGALLRDFPNRWEYYLRQLQNKQFKRIRKCSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDMETEEVEDILQEFVNKSLLFCDRNGKSFRYYLHDLQVDFLTEKNRNQLQDLHRKIITQFQRHHRLHTLSPDQEDCMYWYNFLAYHMASANMHKELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKEISVCSQDCSVCENFQEFLSLNGHLLGRQPFPNIVQLGLCEPETSEVYQQAKLQAKQEVDNGTLYLEWINKKNIKNLSRLVVRPHTDAVYHACFSGDGQRIASCGADKTLQVFKAETGEKLLEIKAHEDEVLCCAFSTDDRFIATCSVDKKVKIWNSMTGELVHTYDEHSEQVNCCHFTNNSHHLLLATGSSDSFLKLWDLNEKDCRNTMFGHTNSVNHCRFSPDDKLLASCSADGSLKLWDVKSANERKSINVKQFFISSEEPQEDMEVIVKCCSWSSDGARIMVAAKNKIFLVDVHTCGLLAEIHTGHHSTIQYCDFSPSNHLAVVALSQYCVELWNMDSCLKVADCRGHLSWVHSVMFSPDGSSFLTSSDDETIRLWETKKVCKNSGIVLKQEIDVLFQENEVMILAVDNMRRLQLINGKTGQIDYLTEAQVSSCCLSPNLQYVALGGEDGAIKILELLNNRIFQSKIGHKSAVRHVQFTTVGKTLISSSDDSVIQVWNWQSEEYVFLQAHQETVKDFRLLRNSRLLSWSFDGTVKVWNIITGRIEKDFVCHQDTVLSCDISPDATKFSSTSADKTAKIWSFELLSPLHELRGHKGCVRCSVFSGDSTLLATGDDNGEIRIWNVSNGELLHLCAPISVEEGAATHGGWVTDLCFSPDSKMLVSAEGSLKWWNAVTGESSQTFYTNGTNLKKIHVSPDFKTYVTVDNLGILYILQVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IA86</accession>
    <name>A0A9L0IA86_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Small nuclear ribonucleoprotein polypeptide A'</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SNRPA1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in pre-mRNA splicing as component of the spliceosome. Associated with sn-RNP U2, where it contributes to the binding of stem loop IV of U2 snRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Identified in the spliceosome B complex. Identified in the spliceosome C complex. Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B. Contributes to the binding of stem loop IV of U2 snRNA with SNRPB2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the U2 small nuclear ribonucleoprotein A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IA86"/>
    <dbReference type="Ensembl" id="ENSEAST00005057392.1">
        <property type="protein sequence ID" value="ENSEASP00005037056.1"/>
        <property type="gene ID" value="ENSEASG00005017706.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153289"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000026">
        <property type="entry name" value="U2 small nuclear ribonucleoprotein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044640">
        <property type="entry name" value="RU2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003603">
        <property type="entry name" value="U2A'_phosphoprotein32A_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10552:SF6">
        <property type="entry name" value="U2 SMALL NUCLEAR RIBONUCLEOPROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00446">
        <property type="entry name" value="LRRcap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="U2A'/phosphoprotein 32 family A C-terminal" evidence="11">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="172"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="221"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="238"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005037056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="253" mass="27940" checksum="0D3412E80E9AC5A7" modified="2023-09-13" version="1">MIEEPCSGTKAIVSSIFPSSELQFSGYKIPVIENLGATLDQFDAIDFSDNEIRKLDGFPLLRRLKTLLVNNNRICRIGEGLDQALPCLTELILTNNSLVELGDLDPLASLKSLTYLSILRNPVTNKKHYRLYVIYKVPQVRVLDFQKVKLKERQEAEKMFKGKRGAQLAKDIARRSKTFNPGAGLPTDKKKGGPSPGDVEAIKNAIANASTLAEVERLKGLLQAGQIPGRERRSGPTDDGEEEMEEDTVTNGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IB22</accession>
    <name>A0A9L0IB22_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Homeobox A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HOXA1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Antp homeobox family. Labial subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014696503.2">
        <property type="nucleotide sequence ID" value="XM_014841017.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IB22"/>
    <dbReference type="Ensembl" id="ENSEAST00005062355.1">
        <property type="protein sequence ID" value="ENSEASP00005037396.1"/>
        <property type="gene ID" value="ENSEASG00005030120.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106831027"/>
    <dbReference type="KEGG" id="eai:106831027"/>
    <dbReference type="CTD" id="3198"/>
    <dbReference type="GeneTree" id="ENSGT00940000157315"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000113">
        <property type="entry name" value="homeobox protein Hox-B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046327">
        <property type="entry name" value="HXA1/B1/D1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45946:SF3">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45946">
        <property type="entry name" value="HOMEOBOX PROTEIN ROUGH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="229"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="231"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="60"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="285"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="315"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005037396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="337" mass="36689" checksum="D9FF739CBA1189A4" modified="2023-09-13" version="1">MDNARMSSFLEYPILSGGDSGTCSARAYPSDHGITTFQSCAVSANSCGGDDRFLVGRGVQISPPHHHHHHHHHHHPQPATYQTPGNLGVSYSHSSCGPSYGGAQNFSAPYSPYALNQEAEVSGGYPSCAPAVYSGNLSSPMVQHHHHHQGYAGGAVGSPQYIHHSYGQEHQSLALATYNNSLSPLHASHQEACRSPTSETSSPAQTFDWMKVKRNPPKTGKVGEYGYVGQPNAVRTNFTTKQLTELEKEFHFNKYLTRARRVEIAASLQLNETQVKIWFQNRRMKQKKREKEGLLPISPATPPGSEEKAEESSEKSSSSPCVPSPGSSTSDTLTTSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IBK5</accession>
    <name>A0A9L0IBK5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106835972</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Cell membrane</location>
            <topology evidence="2 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014704086.2">
        <property type="nucleotide sequence ID" value="XM_014848600.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IBK5"/>
    <dbReference type="Ensembl" id="ENSEAST00005056532.1">
        <property type="protein sequence ID" value="ENSEASP00005035150.1"/>
        <property type="gene ID" value="ENSEASG00005026334.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106835972"/>
    <dbReference type="KEGG" id="eai:106835972"/>
    <dbReference type="GeneTree" id="ENSGT01040000240383"/>
    <dbReference type="OMA" id="CMRNKDV"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000646">
        <property type="entry name" value="Olfactory receptor 887"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:001035">
        <property type="entry name" value="Putative olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="140"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="242"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005035150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="310" mass="34439" checksum="FDA9997C9DA216FE" modified="2023-09-13" version="1">MAAGNHSTVSEFILAGLTEQPELQLPLFLLFLGIYAVTVVGNLGMITLIGLSSHLHTPMYYFLSNLSFIDFCQSSVITPKMLVSFVTEKNIISYPECMTQLYFFLIFAVSECYMLAAMAYDRYVAVCSPLLCSVIMSHQVCFSLILGVYIIGLVCAFAHAGCMFRIHFCNIDVISHYFCDLLPLLKLSCSSTYVNKLLILCVGTLNIFVPSLIILSSYSFITASILRIHSTEGRSKAFSTCSSHMLAVLLFFGSAAFTYLQPSSVTFMNQGKVSSVFYTIVVPMLNPLIYCMRNKDVNVALKKMLKGRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IEG1</accession>
    <name>A0A9L0IEG1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Complex I-B17</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">NADH-ubiquinone oxidoreductase B17 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">NDUFB6</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFB6 subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IEG1"/>
    <dbReference type="Ensembl" id="ENSEAST00005038386.1">
        <property type="protein sequence ID" value="ENSEASP00005039315.1"/>
        <property type="gene ID" value="ENSEASG00005004574.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007535"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019174">
        <property type="entry name" value="NADH_DH_b-subcmplx_su6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15083">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15083:SF0">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 BETA SUBCOMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09782">
        <property type="entry name" value="NDUF_B6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="14 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="8 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00030214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="113" mass="13918" checksum="0488714F7822376C" modified="2023-09-13" version="1">MSGYTPDEKLRLQQLRELRRRWLKDQELSPREPLLPPRRMWPMEEFWNKFLQDKAPWKNMLYKTYRHGIFAFTHVLIPAWIIHYYVKYHVTGDTILETGEVIPPMRDFPDQHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IGN4</accession>
    <name>A0A9L0IGN4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="8">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">USP14</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family. USP14/UBP6 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0IGN4"/>
    <dbReference type="Ensembl" id="ENSEAST00005075604.1">
        <property type="protein sequence ID" value="ENSEASP00005040043.1"/>
        <property type="gene ID" value="ENSEASG00005002507.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009615"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099523">
        <property type="term" value="C:presynaptic cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061136">
        <property type="term" value="P:regulation of proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02657">
        <property type="entry name" value="Peptidase_C19A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16104">
        <property type="entry name" value="Ubl_USP14_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000119">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000032">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044635">
        <property type="entry name" value="UBP14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982:SF1">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="10">
        <location>
            <begin position="121"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00000707"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008739"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
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            <dbReference type="ARBA" id="ARBA00022786"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005040043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="510" mass="57578" checksum="8DD969DEF125E9B6" modified="2023-09-13" version="1">MPLYSVPSPAARGSALPPGPPVTVKWGKEKFEGVELNTDEPPMVFKAQLFALTGVQPARQKVMVKGGTLKDDDWGNIKIKNGMTLLMMGSADALPEEPSAKTVFVEDMTEEQLASAMELPCGLTNLGNTCYMNATVQCIRSVPELKDALKRYAGALRASGEMASAQYITAALRDLFDSMDKTSSSIPPIILLQFLHMAFPQFAEKGEQGQYLQQDANECWIQMMRVLQQKLEAIEDDSVKETDSSSASAVTPSKKKSLIDQFFGVEFETTMKCTESEEEEVTKGRENQLQLSCFINQEVKYLFTGLKLRLQEEITKQSPTLQRNALYIKSSKISRLPAYLTIQMVRFFYKEKESVNAKVLKDVKFPLMLDVYELCTPELQEKMISFRSKFKDLEDKKVNQQTQTSDKKSSPQKEVKYEPFSFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVKRKQDEWIKFDDDKVSIVTPEDILRLSGGGDWHIAYVLLYGPRRVEIMEEESEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IJ93</accession>
    <name>A0A9L0IJ93_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Chimaerin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Chimerin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CHN2</name>
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    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">GTPase-activating protein for p21-rac.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014696531.1">
        <property type="nucleotide sequence ID" value="XM_014841045.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IJ93"/>
    <dbReference type="Ensembl" id="ENSEAST00005044392.1">
        <property type="protein sequence ID" value="ENSEASP00005040958.1"/>
        <property type="gene ID" value="ENSEASG00005000230.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831051"/>
    <dbReference type="KEGG" id="eai:106831051"/>
    <dbReference type="CTD" id="1124"/>
    <dbReference type="GeneTree" id="ENSGT01030000234635"/>
    <dbReference type="OMA" id="HCKTIFA"/>
    <dbReference type="OrthoDB" id="3196451at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20857">
        <property type="entry name" value="C1_betaCHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04372">
        <property type="entry name" value="RhoGAP_chimaerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10352">
        <property type="entry name" value="SH2_a2chimerin_b2chimerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000005">
        <property type="entry name" value="Chimaerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000019">
        <property type="entry name" value="Chimaerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.20:FF:000025">
        <property type="entry name" value="Chimaerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035840">
        <property type="entry name" value="Chimaerin_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017356">
        <property type="entry name" value="CHN1/CHN2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051854">
        <property type="entry name" value="Rho-type_GAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037860">
        <property type="entry name" value="RhoGAP_chimaerin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46075:SF4">
        <property type="entry name" value="BETA-CHIMAERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46075">
        <property type="entry name" value="CHIMERIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038015">
        <property type="entry name" value="N-chimaerin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00324">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 8" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SH2" evidence="11">
        <location>
            <begin position="59"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="12">
        <location>
            <begin position="214"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="13">
        <location>
            <begin position="277"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR038015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005040958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="468" mass="53861" checksum="02624E92F01AA276" modified="2023-09-13" version="1">MAASSNSSLSGSSVSSDAEEYQPPIWKSYLYQLQQEAPRPKRIICPGEVENRPKYYGREFHGIISREQADELLGGVEGAYILRESQRQPGCYTLALRFGNQTLNYRLFYDGKHFVGEKRFESIHDLVTDGLITLYIETKASEYISKMTTNPIYEHIGYATLLREKVSRRLSRSKNESRKTNVTNEEHTAVEKISSLVRRAALTHNDNHFNYEKTHNFKVHTFRGPHWCEYCANFMWGLIAQGVRCSDCGLNVHKQCSKHVPNDCQPDLKRIKKVYCCDLTTLVKAHNTQRPMVVDICIREIEARGLKSEGLYRVSGFTEHIEDVKMAFDRDGEKADISANIYPDINIITGALKLYFRDLPIPVITYDTYSKFIEAAKISNADERLEAVHEVLMLLPPAHYETLRYLMIHLKKVTMNEKDNFMNAENLGIVFGPTLMRPPEDSTLTTLHDMRYQKLIVQILIENEDVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IKH7</accession>
    <name>A0A9L0IKH7_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="Y."/>
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                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="D.D."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IKH7"/>
    <dbReference type="Ensembl" id="ENSEAST00005052488.1">
        <property type="protein sequence ID" value="ENSEASP00005041450.1"/>
        <property type="gene ID" value="ENSEASG00005037586.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000235064"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005041450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="75" mass="8312" checksum="6CE7ABBDB715B57F" modified="2023-09-13" version="1">MVGMKFSRMMMERICFQSVVLSPSSRQMDSSASFTAAGGLAMDRTSTRCCFFMDFTAEHSRSRGGWGVGGRQQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IMN8</accession>
    <name>A0A9L0IMN8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15 17">Serine/threonine-protein phosphatase 2A activator</fullName>
            <ecNumber evidence="5 17">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Phosphotyrosyl phosphatase activator</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">PTPA</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine-protein phosphatase 2A (PP2A). Modulates PP2A activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg(2+). Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg(2+) (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activity of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 17">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the PTPA-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="5 17"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0IMN8"/>
    <dbReference type="Ensembl" id="ENSEAST00005054657.1">
        <property type="protein sequence ID" value="ENSEASP00005039035.1"/>
        <property type="gene ID" value="ENSEASG00005008338.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011500"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904949">
        <property type="term" value="C:ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034704">
        <property type="term" value="C:calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051721">
        <property type="term" value="F:protein phosphatase 2A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008160">
        <property type="term" value="F:protein tyrosine phosphatase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd04087">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1150:FF:000001">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004327">
        <property type="entry name" value="Phstyr_phstse_ac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043170">
        <property type="entry name" value="PTPA_C_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037218">
        <property type="entry name" value="PTPA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03095">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140984">
        <property type="entry name" value="PTPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 17" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13 17" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 17" id="KW-0697">Rotamase</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00044786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00054769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU361210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="389" mass="43766" checksum="9005F8632CD3BD0B" modified="2023-09-13" version="1">MSEQPSPMPPREFLWPHTVPQEPSDKPKEADQLDGVGEVALALAETAQAERTKGVCQRTTVREALPVSLLLGSGSKDSSEDAPAATQNFIIPKKEIHTVPDMGKWKRSQAYADYIGFILTLNEGVKGKKLTFEYTVSEAIEKLVALLNTLDRWIDETPPVDQPSRFGNKAYRTWYAKLDQEAESLVATVVPTHLAAAVPEVAVYLKESVGNSTRIDYGTGHEAAFAAFLCCLCKIGVLRVDDQIAIVFKVFNRYLEVMRKLQKTYRMEPAGSQGVWGLDDFQFLPFIWGSSQLIDHPYLEPRHFVDEKAVNENHKDYMFLECILFITEMKTGPFAEHSNQLWNISAVPSWSKVNQGLIRMYKAECLEKFPVIQHFKFGSLLPIHPVTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IP70</accession>
    <name>A0A9L0IP70_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 17">NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial</fullName>
            <ecNumber evidence="4 17">7.1.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">NDUFV1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29091"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="7.1.1.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:29092"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 17">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="15">Core subunit of respiratory chain NADH dehydrogenase (Complex I) which is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme. Interacts with RAB5IF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Mitochondrion inner membrane</location>
            <topology evidence="17">Peripheral membrane protein</topology>
            <orientation evidence="17">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 17">Belongs to the complex I 51 kDa subunit family.</text>
    </comment>
    <dbReference type="EC" id="7.1.1.2" evidence="4 17"/>
    <dbReference type="RefSeq" id="XP_014700180.1">
        <property type="nucleotide sequence ID" value="XM_014844694.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A9L0IP70"/>
    <dbReference type="SMR" id="A0A9L0IP70"/>
    <dbReference type="Ensembl" id="ENSEAST00005076670.1">
        <property type="protein sequence ID" value="ENSEASP00005041922.1"/>
        <property type="gene ID" value="ENSEASG00005005962.2"/>
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    <dbReference type="GeneID" id="106833485"/>
    <dbReference type="KEGG" id="eai:106833485"/>
    <dbReference type="CTD" id="4723"/>
    <dbReference type="GeneTree" id="ENSGT00390000010641"/>
    <dbReference type="OMA" id="QGDGKPH"/>
    <dbReference type="OrthoDB" id="42889at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
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        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006120">
        <property type="term" value="P:mitochondrial electron transport, NADH to ubiquinone"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1440.230:FF:000001">
        <property type="entry name" value="Mitochondrial NADH dehydrogenase flavoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.600:FF:000001">
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    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11540:FF:000001">
        <property type="entry name" value="NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NADH-ubiquinone oxidoreductase 51kDa subunit"/>
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        <property type="entry name" value="Nuo51_FMN-bd"/>
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        <property type="entry name" value="Nuop51_4Fe4S-bd"/>
    </dbReference>
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        <property type="entry name" value="Nuop51_4Fe4S-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054765">
        <property type="entry name" value="SLBB_dom"/>
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    <dbReference type="NCBIfam" id="TIGR01959">
        <property type="entry name" value="nuoF_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010120">
        <property type="entry name" value="PRK13596.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780:SF10">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN 1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11780">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE FLAVOPROTEIN 1 NDUFV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01512">
        <property type="entry name" value="Complex1_51K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10589">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22461">
        <property type="entry name" value="SLBB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00928">
        <property type="entry name" value="NADH_4Fe-4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142019">
        <property type="entry name" value="Nqo1 FMN-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142984">
        <property type="entry name" value="Nqo1 middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140490">
        <property type="entry name" value="Nqo1C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00644">
        <property type="entry name" value="COMPLEX1_51K_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00645">
        <property type="entry name" value="COMPLEX1_51K_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 17" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="17" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7 17" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 17" id="KW-0288">FMN</keyword>
    <keyword evidence="11 17" id="KW-0408">Iron</keyword>
    <keyword evidence="12 17" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13 17" id="KW-0520">NAD</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="17" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="10" id="KW-1278">Translocase</keyword>
    <keyword evidence="17" id="KW-0813">Transport</keyword>
    <keyword evidence="14" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="NADH-ubiquinone oxidoreductase 51kDa subunit iron-sulphur binding" evidence="18">
        <location>
            <begin position="364"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023075"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00046881"/>
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    </evidence>
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        <source>
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    </evidence>
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        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005041922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
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    <name>A0A9L0IPN2_EQUAS</name>
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        <submittedName>
            <fullName evidence="10">G protein-coupled receptor 180</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GPR180</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014720341.1">
        <property type="nucleotide sequence ID" value="XM_014864855.3"/>
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        <property type="gene ID" value="ENSEASG00005013616.2"/>
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    <dbReference type="KEGG" id="eai:106846223"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
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    <reference evidence="20" key="3">
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        <text evidence="15">Interacts with NHERF1. Interacts with GABARAPL1.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014694256.1">
        <property type="nucleotide sequence ID" value="XM_014838770.3"/>
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        <property type="protein sequence ID" value="ENSEASP00005043156.1"/>
        <property type="gene ID" value="ENSEASG00005026342.1"/>
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    <dbReference type="GeneID" id="106829585"/>
    <dbReference type="KEGG" id="eai:106829585"/>
    <dbReference type="CTD" id="4986"/>
    <dbReference type="GeneTree" id="ENSGT00940000157341"/>
    <dbReference type="OrthoDB" id="6076970at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
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        <property type="term" value="C:cytosol"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:nucleoplasm"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:dynorphin receptor activity"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:neuropeptide binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:adenylate cyclase-inhibiting opioid receptor signaling pathway"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:defense response to virus"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:immune response"/>
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        <property type="term" value="P:locomotory behavior"/>
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        <property type="term" value="P:regulation of saliva secretion"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:sensory perception of pain"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="CDD" id="cd15091">
        <property type="entry name" value="7tmA_Kappa_opioid_R"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Kappa-type opioid receptor 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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    <dbReference type="InterPro" id="IPR000452">
        <property type="entry name" value="Kappa_opi_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001418">
        <property type="entry name" value="Opioid_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229:SF1">
        <property type="entry name" value="KAPPA-TYPE OPIOID RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24229">
        <property type="entry name" value="NEUROPEPTIDES RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00532">
        <property type="entry name" value="KAPPAOPIOIDR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00384">
        <property type="entry name" value="OPIOIDR"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0085">Behavior</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 17" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="11 17" id="KW-0675">Receptor</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 17" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="57"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="97"/>
            <end position="119"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="139"/>
            <end position="159"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="180"/>
            <end position="199"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="224"/>
            <end position="251"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="272"/>
            <end position="296"/>
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        <location>
            <begin position="311"/>
            <end position="333"/>
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    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="19">
        <location>
            <begin position="76"/>
            <end position="330"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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        <source>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022610"/>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
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    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
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    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005043156.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="380" mass="42688" checksum="7843CCCBD9742AF9" modified="2023-09-13" version="1">MESPVQIFRGEPGPTCAPSTCLLPNDSGWFPGWAEPDGNSSAGSEDAPLEPAHISPAIPVIITAVYSVVFVVGLVGNSLVMFVIIRYTKMKTATNIYIFNLALADALVTTTMPFQSTVYLMNSWPFGDVLCKIVISIDYYNMFTSIFTLTMMSVDRYIAVCHPVKALDFRTPLKAKIINICIWLLSSSVGISAIVLGGTKVREDVDVIECSLQFPDDDYSWWDLFMKICVFIFAFVIPVLIIIICYTLMILRLKSVRLLSGSREKDRNLRRITRLVLVVVAVFIICWTPIHIFILVEALGSTSHSTAALSSYYFCIALGYTNSSLNPILYAFLDENFKRCFRDFCFPIKMRMERQSTSRVRNTLQDPAYMKDVDGINKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IRC0</accession>
    <name>A0A9L0IRC0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Phosphomannomutase</fullName>
            <ecNumber evidence="14">5.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PMM2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="2 14">Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 14">
            <text>alpha-D-mannose 1-phosphate = D-mannose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11140"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="ChEBI" id="CHEBI:58735"/>
            <dbReference type="EC" id="5.4.2.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 14">Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 14">Belongs to the eukaryotic PMM family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.8" evidence="14"/>
    <dbReference type="RefSeq" id="XP_014705436.2">
        <property type="nucleotide sequence ID" value="XM_014849950.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IRC0"/>
    <dbReference type="Ensembl" id="ENSEAST00005058747.1">
        <property type="protein sequence ID" value="ENSEASP00005039629.1"/>
        <property type="gene ID" value="ENSEASG00005011577.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106836768"/>
    <dbReference type="KEGG" id="eai:106836768"/>
    <dbReference type="CTD" id="5373"/>
    <dbReference type="GeneTree" id="ENSGT00390000002918"/>
    <dbReference type="OMA" id="ISHRVYT"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004615">
        <property type="term" value="F:phosphomannomutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061729">
        <property type="term" value="P:GDP-D-mannose biosynthetic process from fructose-6-phosphate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061728">
        <property type="term" value="P:GDP-mannose biosynthetic process from mannose"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02585">
        <property type="entry name" value="HAD_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1240.20:FF:000001">
        <property type="entry name" value="Phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000216">
        <property type="entry name" value="Phosphomannomutase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1240.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
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    <dbReference type="InterPro" id="IPR043169">
        <property type="entry name" value="PMM_cap"/>
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    <dbReference type="NCBIfam" id="TIGR01484">
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        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR10466">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
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    <dbReference type="PANTHER" id="PTHR10466:SF2">
        <property type="entry name" value="PHOSPHOMANNOMUTASE 2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF03332">
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        <property type="match status" value="1"/>
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    <dbReference type="SFLD" id="SFLDF00445">
        <property type="entry name" value="alpha-phosphomannomutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01143">
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="51"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
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        <location>
            <position position="53"/>
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        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
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        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
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    <feature type="binding site" evidence="12">
        <location>
            <position position="173"/>
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        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
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        <location>
            <position position="180"/>
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            <name>alpha-D-mannose 1-phosphate</name>
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        <location>
            <position position="218"/>
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        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
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        <location>
            <position position="220"/>
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        <ligand>
            <name>alpha-D-mannose 1-phosphate</name>
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        <location>
            <position position="248"/>
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            <position position="262"/>
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        <location>
            <position position="265"/>
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        <ligand>
            <name>Mg(2+)</name>
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        <source>
            <dbReference type="ARBA" id="ARBA00000586"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00002564"/>
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            <dbReference type="ARBA" id="ARBA00004496"/>
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            <dbReference type="ARBA" id="ARBA00004699"/>
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            <dbReference type="ARBA" id="ARBA00011738"/>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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            <dbReference type="ARBA" id="ARBA00023235"/>
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        <source>
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            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="285" mass="32029" checksum="8A64B2D4FFCFC065" modified="2023-09-13" version="1">MRADAPPSGVPCGRGLNPEVPGRVPGANESCQVCEKARDMAAPGPALCLFDVDGTLTPPRQKITKEMDDFLQKLRQKIKIGVVGGSDFEKVQEQLGNDVVEKYDYVFPENGLVAYKDGKLVCKQSIQGHLGEALIQDLINYCLSYISKIKLPKKRGTFIEFRNGMLNVSPIGRSCSQEERIEFHELDKKENIRQKFVADLQREFAGKGLTFSIGGQISFDVFPEGWDKRYCLRHVEKDGYKTIYFFGDKTMPGGNDHEIFTDPRTVGYTVTAPEDTRRICEELFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IS45</accession>
    <name>A0A9L0IS45_EQUAS</name>
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        <recommendedName>
            <fullName evidence="10">Death domain-containing membrane protein NRADD</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Neurotrophin receptor homolog-2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Neurotrophin receptor-alike death domain protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106823257</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type III membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014828842.3"/>
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        <property type="protein sequence ID" value="ENSEASP00005040596.1"/>
        <property type="gene ID" value="ENSEASG00005008169.2"/>
    </dbReference>
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    <dbReference type="KEGG" id="eai:106823257"/>
    <dbReference type="GeneTree" id="ENSGT00940000163571"/>
    <dbReference type="OrthoDB" id="10061577at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030027">
        <property type="term" value="C:lamellipodium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005641">
        <property type="term" value="C:nuclear envelope lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015026">
        <property type="term" value="F:coreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005035">
        <property type="term" value="F:death receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048406">
        <property type="term" value="F:nerve growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007266">
        <property type="term" value="P:Rho protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000140">
        <property type="entry name" value="Death domain-containing membrane protein NRADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1780">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000488">
        <property type="entry name" value="Death_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052302">
        <property type="entry name" value="Neurotrophin_rcpt-DD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041448">
        <property type="entry name" value="TNFR16_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46605:SF1">
        <property type="entry name" value="DEATH DOMAIN-CONTAINING MEMBRANE PROTEIN NRADD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46605">
        <property type="entry name" value="TUMOR NECROSIS FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00531">
        <property type="entry name" value="Death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18422">
        <property type="entry name" value="TNFR_16_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00005">
        <property type="entry name" value="DEATH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50017">
        <property type="entry name" value="DEATH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Death" evidence="14">
        <location>
            <begin position="264"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00081527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005040596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="334" mass="36160" checksum="78E9D9A4E28F30F5" modified="2023-09-13" version="1">MGPALLPPPPPSDTGGNWSRLGSGEVLRGTQSWSESEWSEPGERDRLPQRTSIVRPRDFSPCPYPQPILDSEKSPNYLASVPLIILQPLLQEMQPLGALLAGRAMLHNSSHREGMVHVDKTWRGQDMDREGVWVEAGGALAPNTSSPFPPEPPGASGSIIPVYCALLATVVLGLLAYVAFKCWRSHKQRQQLAKARTAELGALDRDQIHGDSGVFLDSPSSLPCAPSQGPYPELGCRLYLHLPRQQQEEVERLLELSGEPDKGWQGLAGRLGYQAEVVETMAQGQAPAYTLLRDWAVQEGSGATLRVLEVALAAMGREDVVRALGPLAEGCSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IT57</accession>
    <name>A0A9L0IT57_EQUAS</name>
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        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="11" type="primary">LOC106821836</name>
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        <name type="scientific">Equus asinus</name>
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        <lineage>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IT57"/>
    <dbReference type="Ensembl" id="ENSEAST00005053618.1">
        <property type="protein sequence ID" value="ENSEASP00005044054.1"/>
        <property type="gene ID" value="ENSEASG00005025938.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282514"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0004930">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="7tmA_OR5D-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="142"/>
            <end position="168"/>
        </location>
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            <begin position="204"/>
            <end position="229"/>
        </location>
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            <begin position="241"/>
            <end position="265"/>
        </location>
    </feature>
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        <location>
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            <end position="296"/>
        </location>
    </feature>
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        <location>
            <begin position="43"/>
            <end position="294"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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</entry>
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    <accession>A0A9L0ITK8</accession>
    <name>A0A9L0ITK8_EQUAS</name>
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        </alternativeName>
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        </alternativeName>
    </protein>
    <gene>
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    </gene>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Yang"/>
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                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
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            <dbReference type="ChEBI" id="CHEBI:61977"/>
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        </reaction>
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    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
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    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0ITK8"/>
    <dbReference type="Ensembl" id="ENSEAST00005065427.1">
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        <property type="gene ID" value="ENSEASG00005010042.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159871"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0044297">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008200">
        <property type="term" value="F:ion channel inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035932">
        <property type="term" value="P:aldosterone secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055074">
        <property type="term" value="P:calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0071466">
        <property type="term" value="P:cellular response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006821">
        <property type="term" value="P:chloride transport"/>
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        <property type="project" value="Ensembl"/>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
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                <person name="Huang"/>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
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                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
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                <person name="R."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0051371">
        <property type="term" value="F:muscle alpha-actinin binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005080">
        <property type="term" value="F:protein kinase C binding"/>
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        <property type="term" value="P:heart development"/>
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    <dbReference type="GO" id="GO:0045214">
        <property type="term" value="P:sarcomere organization"/>
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    <dbReference type="CDD" id="cd09454">
        <property type="entry name" value="LIM1_ZASP_Cypher"/>
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        <property type="entry name" value="LIM2_Enigma_like"/>
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        <property type="entry name" value="LIM3_ZASP_Cypher"/>
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        <property type="entry name" value="PDLI1-4/Zasp-like_mid"/>
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    <dbReference type="InterPro" id="IPR006643">
        <property type="entry name" value="Zasp-like_motif"/>
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    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24214:SF9">
        <property type="entry name" value="LIM DOMAIN-BINDING PROTEIN 3"/>
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    <dbReference type="PANTHER" id="PTHR24214">
        <property type="entry name" value="PDZ AND LIM DOMAIN PROTEIN ZASP"/>
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    <dbReference type="Pfam" id="PF15936">
        <property type="entry name" value="DUF4749"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
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        <property type="entry name" value="LIM"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="PDZ"/>
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        <property type="entry name" value="ZM"/>
        <property type="match status" value="2"/>
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        <property type="match status" value="4"/>
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        <property type="entry name" value="LIM_DOMAIN_1"/>
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        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="PDZ"/>
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    <keyword evidence="6 7" id="KW-0440">LIM domain</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
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    </feature>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IYQ3</accession>
    <name>A0A9L0IYQ3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Interleukin 6 receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">IL6R</name>
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    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 3 subfamily.</text>
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        <property type="nucleotide sequence ID" value="XM_070497647.1"/>
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        <property type="gene ID" value="ENSEASG00005000344.2"/>
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        <property type="component" value="Chromosome 25"/>
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        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="C:external side of plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005896">
        <property type="term" value="C:interleukin-6 receptor complex"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:ciliary neurotrophic factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004897">
        <property type="term" value="F:ciliary neurotrophic factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019970">
        <property type="term" value="F:interleukin-11 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004921">
        <property type="term" value="F:interleukin-11 receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0019981">
        <property type="term" value="F:interleukin-6 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004915">
        <property type="term" value="F:interleukin-6 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005138">
        <property type="term" value="F:interleukin-6 receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097696">
        <property type="term" value="P:cell surface receptor signaling pathway via STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031018">
        <property type="term" value="P:endocrine pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032722">
        <property type="term" value="P:positive regulation of chemokine production"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072126">
        <property type="term" value="P:positive regulation of glomerular mesangial cell proliferation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032755">
        <property type="term" value="P:positive regulation of interleukin-6 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048661">
        <property type="term" value="P:positive regulation of smooth muscle cell proliferation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072540">
        <property type="term" value="P:T-helper 17 cell lineage commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010573">
        <property type="term" value="P:vascular endothelial growth factor production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ciliary neurotrophic factor receptor alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Interleukin 6 receptor"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
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    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
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    <dbReference type="InterPro" id="IPR003530">
        <property type="entry name" value="Hematopoietin_rcpt_L_F3_CS"/>
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    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Type-I_Cytokine_Rcpt"/>
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        <property type="entry name" value="TypeI_recpt_CBD"/>
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    <dbReference type="PANTHER" id="PTHR23036">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23036:SF98">
        <property type="entry name" value="INTERLEUKIN-6 RECEPTOR SUBUNIT ALPHA"/>
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    <dbReference type="Pfam" id="PF00047">
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    <dbReference type="SMART" id="SM00408">
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    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
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    <dbReference type="SUPFAM" id="SSF48726">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="21"/>
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            <begin position="22"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="12">
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            <begin position="345"/>
            <end position="361"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="12">
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            <begin position="438"/>
            <end position="452"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
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            <end position="468"/>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="486" mass="53504" checksum="430596022077D028" modified="2023-09-13" version="1">MLAVCCALLTALLAAPGAALAQGGCPALEVASDVLTRLPGASVVLTCPGGEPGDNATFHWMLRSRVIGGPGSHRDRWTVVGRRLVLSSLQLSDSGNYSCYQDGHLVGTVCLLVDVPPEQPQLSCSRKSPLSNVGCEWSPQRPPSPTTKAVLLVKKFAETESEPCPCFVSDSQNSPVENFQEPCLYSQESQKFSCQLAVPEGDNSFYIVSLCVANSAGSESSRSQTFEGYGILQPDPPVNITVTAVDRNPRWLSVTWQDPPSWNSYFYRLHFELRYRAERSKTFTMWMVKELQHHCIIRDAWRGMRHVVQLRAQEEFGHGLWSEWSQEVMGTPWTEPRSPGVETEVSPSTQASTTNEDNENTLFKDSANATSLPVQDSSLTSLPTFLVAGGSLAFGTLLCIGIVLRFKRTWKLQALKEGKTGMHPPYSLGQLVPERPKPTPVLVPLISPPVSPSSPESDNTPRHSRPDARGPQSPYDITNRDYFFPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IZ89</accession>
    <name>A0A9L0IZ89_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Vomeronasal type-1 receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative pheromone receptor.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="gene ID" value="ENSEASG00005038828.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 8"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0016503">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019236">
        <property type="term" value="P:response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007606">
        <property type="term" value="P:sensory perception of chemical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Vomeronasal type-1 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004072">
        <property type="entry name" value="Vmron_rcpt_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24062">
        <property type="entry name" value="VOMERONASAL TYPE-1 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03402">
        <property type="entry name" value="V1R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="174"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="18"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005043067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="303" mass="35559" checksum="3A062EA2EC1D555B" modified="2023-09-13" version="1">MNYSLQIHIITTIILLPMNFVFINYIYIFFQGTEKKSVHLILMHLAFANTIVLFFKGMPKIIATFGLISFLNDTGCKIVIYLERVARGLSLCMTSFLTVVQAITIIPSAPEWAKLKPRSKWHILCFFLFFWIFNSLLSMNLLYYIQSISSMNSSQNSDNNRYCYFIPESQKMKWIFLILMGIRDALCQCLMGWASVYMIFLLHKHHKHVLYLQNSKVLYRTPPEIRAAQSVLLLMLCFLFFYWTDCIFSLYFNSSFDNNFIELNILEILNLGYASLRSPFVSVTRNKLLSKFYSIHCENLSHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J4R0</accession>
    <name>A0A9L0J4R0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C3a anaphylatoxin chemotactic receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">C3AR1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with VGF-derived peptide TLQP-21.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the chemokine-like receptor (CMKLR) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014706465.1">
        <property type="nucleotide sequence ID" value="XM_014850979.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J4R0"/>
    <dbReference type="Ensembl" id="ENSEAST00005054603.1">
        <property type="protein sequence ID" value="ENSEASP00005047363.1"/>
        <property type="gene ID" value="ENSEASG00005026436.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106837440"/>
    <dbReference type="KEGG" id="eai:106837440"/>
    <dbReference type="CTD" id="719"/>
    <dbReference type="GeneTree" id="ENSGT01140000282544"/>
    <dbReference type="OMA" id="MCGYNFG"/>
    <dbReference type="OrthoDB" id="10037617at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004876">
        <property type="term" value="F:complement component C3a receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004878">
        <property type="term" value="F:complement component C5a receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007193">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045766">
        <property type="term" value="P:positive regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010759">
        <property type="term" value="P:positive regulation of macrophage chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090023">
        <property type="term" value="P:positive regulation of neutrophil chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010575">
        <property type="term" value="P:positive regulation of vascular endothelial growth factor production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000269">
        <property type="entry name" value="C3a anaphylatoxin chemotactic receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000284">
        <property type="entry name" value="C3a anaphylatoxin chemotactic receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001644">
        <property type="entry name" value="Anaphtx_C3AR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002234">
        <property type="entry name" value="Anphylx_rcpt_C3a/C5a1-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000826">
        <property type="entry name" value="Formyl_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225:SF28">
        <property type="entry name" value="C3A ANAPHYLATOXIN CHEMOTACTIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24225">
        <property type="entry name" value="CHEMOTACTIC RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01104">
        <property type="entry name" value="ANPHYLATOXNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01060">
        <property type="entry name" value="C3ANPHYLTXNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9 18" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12 18" id="KW-0675">Receptor</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0765">Sulfation</keyword>
    <keyword evidence="14 18" id="KW-0807">Transducer</keyword>
    <keyword evidence="7 18 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="23"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="60"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="90"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="139"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="341"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="415"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="20">
        <location>
            <begin position="40"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00025736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005047363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="484" mass="53853" checksum="6E336412410F6210" modified="2023-09-13" version="1">MESFSAESNSTDLHSQPQNKLQVILSMVILSLTLLLGLPGNGLVLWVAGLKMKRTVNTVWFIHLTVADFLCCLSLPFSLAHLALQGHWPYGWLLCKLIPSIIVLNMFASVFLLTAISLDRCLLVLKPIWCQNHRNVGTAFTICGCIWVVAFVMCIPVFIYREMFSVDNHNMCGYNFGPYSSLDYSDFTFDLLENGSLDNAFVQLPGEMNNRLDSFSLQAGDHPWTATTVLHSQTFQRPSGDSLSTDSARLSNQHLYYNLFKPADEISPTGPGGFPIEDHRINPLDNSDASLSTDLELFSNASSNSFYTYEPSQDFLDDNLGQFAYSNQMSTPLVAITITRLVVGFLGPFLIMVACYSLIILRMRRGRLTKSRSKTLQVAMVVVVVFFVCWAPYHIVGVLLLFIDSETPFGEALLAWDHVSIALASANSCFNPFLYALLGKDFRKKARQSMQGILEAAFSEELTHSTSCTPSKVFSEKNSIGTVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J698</accession>
    <name>A0A9L0J698_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">tissue kallikrein</fullName>
            <ecNumber evidence="8">3.4.21.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">KLK5</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.</text>
            <dbReference type="EC" id="3.4.21.35"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S1 family. Snake venom subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.35" evidence="8"/>
    <dbReference type="RefSeq" id="XP_044614798.1">
        <property type="nucleotide sequence ID" value="XM_044758863.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J698"/>
    <dbReference type="Ensembl" id="ENSEAST00005075324.1">
        <property type="protein sequence ID" value="ENSEASP00005044770.1"/>
        <property type="gene ID" value="ENSEASG00005029518.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106840855"/>
    <dbReference type="KEGG" id="eai:106840855"/>
    <dbReference type="CTD" id="25818"/>
    <dbReference type="GeneTree" id="ENSGT01020000230389"/>
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        <property type="component" value="Chromosome 26"/>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
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                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of cytokine production"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="complement C1q tumor necrosis factor-related protein 3 isoform X2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="C1q_dom"/>
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    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050392">
        <property type="entry name" value="Collagen/C1q_domain"/>
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    <dbReference type="InterPro" id="IPR008983">
        <property type="entry name" value="Tumour_necrosis_fac-like_dom"/>
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    <dbReference type="PANTHER" id="PTHR15427:SF52">
        <property type="entry name" value="C1Q DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15427">
        <property type="entry name" value="EMILIN ELASTIN MICROFIBRIL INTERFACE-LOCATED PROTEIN ELASTIN MICROFIBRIL INTERFACER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00386">
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    <dbReference type="PRINTS" id="PR00007">
        <property type="entry name" value="COMPLEMNTC1Q"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="TNF-like"/>
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    <dbReference type="PROSITE" id="PS50871">
        <property type="entry name" value="C1Q"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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            <begin position="1"/>
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    <feature type="chain" id="PRO_5040261797" description="Complement C1q tumor necrosis factor-related protein 3" evidence="9">
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            <begin position="23"/>
            <end position="319"/>
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        <location>
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        <location>
            <begin position="52"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="8">
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    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="128"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
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        </location>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    <sequence length="319" mass="35188" checksum="3F6CF49D6B27FAA0" modified="2023-09-13" version="1">MLRRQLVYWHLLVLLFLPFCLCQDEYMEVSRRANKVVARIVQSHQQTVHSGSRREKVRERSHPKTGTVDNNTSTDLKSLRPDELPHPEVDDLAQITPFWGQSPQTGGLPPDCSKCCHGDYSFRGYQGPPGPPGPPGIPGNHGNNGNNGATGHEGAKGEKGDKGDLGPRGERGQHGPKGEKGHPGIPPELQIAFMASLATHFTNQNSGIIFSSVETNIGNFFDVMTGRFGAPVSGVYFFTFSMMKHEDVEEVYVYLMHNGNTVFSMYSYETKGKSDTSSNHAVLKLAKGDEVWLRMGNGALHGDHQRFSTFAGFLLFETK</sequence>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Ju"/>
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                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
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                <person name="Liu"/>
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                <person name="Guo"/>
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                <person name="Pan"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Xu"/>
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                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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        <location>
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            <end position="111"/>
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    </feature>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005048946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="111" mass="12102" checksum="BE933941D29F2D14" modified="2023-09-13" version="1">MGTRFLCWVTICLLGAELTEGGVTQSPRHKITGKGQAVALWCDPISGHRALYWYQQTLGQGLGLLISFEDEAVADDSQLPKHRFSAERSKGGDYSPDQACRAGGLSRVFLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J6W0</accession>
    <name>A0A9L0J6W0_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Heat shock protein family B (small) member 1</fullName>
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    </protein>
    <gene>
        <name evidence="7" type="primary">HSPB1</name>
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    <organism evidence="7 8">
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    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="Jin"/>
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                <person name="Yue"/>
                <person name="G."/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
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                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the small heat shock protein (HSP20) family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A9L0J6W0"/>
    <dbReference type="Ensembl" id="ENSEAST00005077991.1">
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    </dbReference>
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        <property type="project" value="GOC"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:protein kinase C binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0008426">
        <property type="term" value="F:protein kinase C inhibitor activity"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099641">
        <property type="term" value="P:anterograde axonal protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035924">
        <property type="term" value="P:cellular response to vascular endothelial growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902176">
        <property type="term" value="P:negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090038">
        <property type="term" value="P:negative regulation of protein kinase C signaling"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045766">
        <property type="term" value="P:positive regulation of angiogenesis"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0043536">
        <property type="term" value="P:positive regulation of blood vessel endothelial cell migration"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001028">
        <property type="term" value="P:positive regulation of endothelial cell chemotaxis"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0032731">
        <property type="term" value="P:positive regulation of interleukin-1 beta production"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0032760">
        <property type="term" value="P:positive regulation of tumor necrosis factor production"/>
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        <property type="project" value="Ensembl"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SHSP" evidence="6">
        <location>
            <begin position="80"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
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                <person name="Jin"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <scope>IDENTIFICATION</scope>
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                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Li"/>
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                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
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    <dbReference type="RefSeq" id="XP_014704906.1">
        <property type="nucleotide sequence ID" value="XM_014849420.3"/>
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        <property type="nucleotide sequence ID" value="XM_044744658.2"/>
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        <property type="nucleotide sequence ID" value="XM_070483406.1"/>
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        <property type="nucleotide sequence ID" value="XM_070483407.1"/>
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        <property type="gene ID" value="ENSEASG00005029916.1"/>
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        <property type="protein sequence ID" value="ENSEASP00005051025.1"/>
        <property type="gene ID" value="ENSEASG00005029916.1"/>
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        <property type="component" value="Chromosome 13"/>
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    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="Reprimo_fam"/>
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    <dbReference type="PANTHER" id="PTHR28649">
        <property type="entry name" value="PROTEIN REPRIMO-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR28649:SF3">
        <property type="entry name" value="REPRIMO-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="67"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005051025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="117" mass="12033" checksum="12089F97C1D85A20" modified="2023-09-13" version="1">MNATFLNHSGLEAAGGGGGAALGNRSHGLGTWLGCCPGGAPLTASDGVPVGLSPDERSLWVSRVAQIAVLCVLSLTVVFGVFFLGCNLLIKSESMINFLMQERRPSKDVGAAILGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JPM8</accession>
    <name>A0A9L0JPM8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC123280259</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the alpha/beta interferon family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JPM8"/>
    <dbReference type="Ensembl" id="ENSEAST00005050669.1">
        <property type="protein sequence ID" value="ENSEASP00005051937.1"/>
        <property type="gene ID" value="ENSEASG00005027698.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01000000214430"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005126">
        <property type="term" value="F:cytokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00095">
        <property type="entry name" value="IFab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.10:FF:000001">
        <property type="entry name" value="Interferon alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000471">
        <property type="entry name" value="Interferon_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691:SF36">
        <property type="entry name" value="IFN-CHI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691">
        <property type="entry name" value="TYPE I INTERFERON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00143">
        <property type="entry name" value="Interferon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00266">
        <property type="entry name" value="INTERFERONAB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00076">
        <property type="entry name" value="IFabd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00252">
        <property type="entry name" value="INTERFERON_A_B_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="2 7" id="KW-0202">Cytokine</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040280988" evidence="8">
        <location>
            <begin position="20"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005051937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="186" mass="20997" checksum="64446A76E6F294F7" modified="2023-09-13" version="1">MALPVSVLLALVMLGSCPACPLGCDPPLRQGNQETITLLSQMERISILSCLKDRADFRFPQILVDGDQLEKTQATAVLHEMLQHIFHLFSTTGSRAAWDETLLDKFLLGLYQQLDDLETCLGKETKAEQSALGSEISTLAVKRYFQRISLYLKEKDYSSCAWEVVRVEIRRCLLFMNKLTGKLNRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="3 10">Zinc finger protein-like 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ZFPL1</name>
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        <name type="common">Donkey</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Required for cis-Golgi integrity and efficient ER to Golgi transport.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with GOLGA2/GM130.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Golgi apparatus</location>
            <location evidence="1 10">cis-Golgi network membrane</location>
            <topology evidence="1 10">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The B box-type and RING-type zinc fingers although degenerate play a central role in function of the protein.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ZFPL1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014700367.1">
        <property type="nucleotide sequence ID" value="XM_014844881.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014700369.1">
        <property type="nucleotide sequence ID" value="XM_014844883.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044618229.1">
        <property type="nucleotide sequence ID" value="XM_044762294.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044618230.1">
        <property type="nucleotide sequence ID" value="XM_044762295.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JYD7"/>
    <dbReference type="Ensembl" id="ENSEAST00005077578.1">
        <property type="protein sequence ID" value="ENSEASP00005058154.1"/>
        <property type="gene ID" value="ENSEASG00005018221.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106833602"/>
    <dbReference type="KEGG" id="eai:106833602"/>
    <dbReference type="CTD" id="7542"/>
    <dbReference type="GeneTree" id="ENSGT00390000009753"/>
    <dbReference type="OMA" id="CEHCMVA"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16487">
        <property type="entry name" value="mRING-H2-C3DHC3_ZFPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058730">
        <property type="entry name" value="U-box_ZFPL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039043">
        <property type="entry name" value="ZFPL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058731">
        <property type="entry name" value="Znf-B_box_ZFPL1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12981">
        <property type="entry name" value="ZINC FINGER PROTEIN-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12981:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25998">
        <property type="entry name" value="U-box_ZFPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25993">
        <property type="entry name" value="zf-B_box_ZFPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <keyword evidence="7 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="ZFPL1-like B-box zinc-binding" evidence="12">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="domain" description="ZFPL1-like U-box" evidence="13">
        <location>
            <begin position="52"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="143"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="202"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="148"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU369078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF25993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF25998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005058154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="312" mass="34254" checksum="378F40C9B4142C30" modified="2023-09-13" version="1">MGLCKCPKRKVTNLFCFEHRVNVCEHCLVANHAKCIVQSYLQWLQDSDYNPNCRLCNIPLATRETTRLVCYDLFHWACLNERAAQLPRNTAPAGYQCPSCSGPIFPPANLAGPVASTLREKLATVNWARAGLGLPLIDEVVSPEPEPLNTSDFSDWSSFNASGTPEQEEIASASAAPAFYSQAPRPPASPSRPEQHTVIHMGNAEPLTHASAPRKVYDTRDDDRAPGFHGDCDDDKYRRRPALGWLAQLLRSRAGSRKQPLTLLQRAGLLLLLGLLGFLALLALMSRLGRAAADSDPNLDPLMNPHIRVGPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K001</accession>
    <name>A0A9L0K001_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106846589</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044609725.1">
        <property type="nucleotide sequence ID" value="XM_044753790.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K001"/>
    <dbReference type="Ensembl" id="ENSEAST00005065955.1">
        <property type="protein sequence ID" value="ENSEASP00005058083.1"/>
        <property type="gene ID" value="ENSEASG00005029787.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106846589"/>
    <dbReference type="KEGG" id="eai:106846589"/>
    <dbReference type="GeneTree" id="ENSGT00950000182755"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002682">
        <property type="term" value="P:regulation of immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002568">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000193">
        <property type="entry name" value="Zinc finger protein 300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000184">
        <property type="entry name" value="Zinc finger protein 333"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000044">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000773">
        <property type="entry name" value="Zinc finger protein 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000371">
        <property type="entry name" value="Zinc finger protein 555"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000292">
        <property type="entry name" value="zinc finger protein 619"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF67">
        <property type="entry name" value="ZINC FINGER PROTEIN 709-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="14">
        <location>
            <begin position="4"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="168"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="196"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="224"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="252"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="280"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="308"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="336"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="364"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="392"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="420"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="448"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="476"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="504"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005058083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="528" mass="61146" checksum="FABE8F108656F74C" modified="2023-09-13" version="1">MDSVAIEDVAVNFTPEEWALLDPSQKKLYRDVMEETFRNLASVGTTWDDRDIKDQYQHEERKPRNPVVVRFCESEDGNQCGENFRCVPNLNLNKKTTGAKPWECTACGRVFMHHSSLYRHVKYHPDHRPRDYQNHREKPYKCKECGKVFRFPSFLQIHERTHTGDKPYECKKCSKAFTYSSSLQIHERGHTGEKPYECKECNKTFSCNSSLQKHDRIHTGKKPFECKICGKAFRFPSNVQAHERTHTGEKPYECKICGKAFSLPQSFKIHQRIHTGEKPYECKECNKTFRCNSSLQKHERIHTREKPYECKKCSKAFISLSLLQEHKRIHTGEKPYECKTCSKAFTSSSSLQIHERIHTGEKSYECKECSKAFGSNSSLQKHERTHTGEKPHECKKCSKAFTSSSSLRVHERTHTGEKPYECKKCSKAFTCSSSLRVHERIHTGEKPYECKGCGKAFSSSGSLQKHGRTHTGEKPYECKLCGKAFRFPSNIQAHERTHTGEKPYECKECGKAFTSSSSLQKHERIHNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K276</accession>
    <name>A0A9L0K276_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Microtubule-associated proteins 1A/1B light chain 3B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">MAP1LC3B</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Autophagosome membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the ATG8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K276"/>
    <dbReference type="Ensembl" id="ENSEAST00005056844.1">
        <property type="protein sequence ID" value="ENSEASP00005059606.1"/>
        <property type="gene ID" value="ENSEASG00005028539.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154158"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006914">
        <property type="term" value="P:autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000059">
        <property type="entry name" value="Microtubule-associated proteins 1A/1B light chain 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005059606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16780" checksum="A7573AC7A1F38CD5" modified="2023-09-13" version="1">MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKDEDGFLYMVDPPSEFPTKCMSMVKKTQQEETRALPDVSGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K2K6</accession>
    <name>A0A9L0K2K6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">tRNA-splicing endonuclease subunit Sen2</fullName>
            <ecNumber evidence="3 9">4.6.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">TSEN2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the tRNA-intron endonuclease family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16" evidence="3 9"/>
    <dbReference type="RefSeq" id="XP_014693879.1">
        <property type="nucleotide sequence ID" value="XM_014838393.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044610584.1">
        <property type="nucleotide sequence ID" value="XM_044754649.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070348700.1">
        <property type="nucleotide sequence ID" value="XM_070492599.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070348701.1">
        <property type="nucleotide sequence ID" value="XM_070492600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K2K6"/>
    <dbReference type="Ensembl" id="ENSEAST00005073840.1">
        <property type="protein sequence ID" value="ENSEASP00005056567.1"/>
        <property type="gene ID" value="ENSEASG00005014763.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106829343"/>
    <dbReference type="KEGG" id="eai:106829343"/>
    <dbReference type="CTD" id="80746"/>
    <dbReference type="GeneTree" id="ENSGT00390000013266"/>
    <dbReference type="OMA" id="LWRRWNP"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000214">
        <property type="term" value="C:tRNA-intron endonuclease complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000379">
        <property type="term" value="P:tRNA-type intron splice site recognition and cleavage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22363">
        <property type="entry name" value="tRNA-intron_lyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR011856">
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        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
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    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
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    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
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            <end position="330"/>
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            <end position="196"/>
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            <dbReference type="ARBA" id="ARBA00022694"/>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K3G8</accession>
    <name>A0A9L0K3G8_EQUAS</name>
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        <recommendedName>
            <fullName evidence="8">Insulin receptor substrate 4</fullName>
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        <alternativeName>
            <fullName evidence="9">Phosphoprotein of 160 kDa</fullName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
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            <topology evidence="1">Peripheral membrane protein</topology>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Insulin receptor substrate 1"/>
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        <property type="entry name" value="insulin receptor substrate 4"/>
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    </dbReference>
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        <property type="entry name" value="IRS_PTB"/>
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        <property type="entry name" value="PH-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
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    <dbReference type="PANTHER" id="PTHR10614">
        <property type="entry name" value="INSULIN RECEPTOR SUBSTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="INSULIN RECEPTOR SUBSTRATE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02174">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00628">
        <property type="entry name" value="INSULINRSI"/>
    </dbReference>
    <dbReference type="SMART" id="SM01244">
        <property type="entry name" value="IRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00310">
        <property type="entry name" value="PTBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51064">
        <property type="entry name" value="IRS_PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="5" id="KW-0832">Ubl conjugation</keyword>
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        <location>
            <begin position="78"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="IRS-type PTB" evidence="12">
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            <end position="335"/>
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            <end position="938"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1184"/>
            <end position="1260"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="495"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="510"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="10">
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    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="800"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="10">
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        <location>
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        <location>
            <begin position="1186"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1240"/>
            <end position="1260"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00068177"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00080657"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50003"/>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005060031.1"/>
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        <source>
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        </source>
    </evidence>
    <sequence length="1260" mass="133278" checksum="F6AD6E6D0E52F650" modified="2023-09-13" version="1">MASCSSARDQATRRGRAAAAATAAALAAVATTPLLSSGTPTALIGTGSSCPGAMWLSTATGSRSDSESDEEDLPVGDEVCKRGYLRKQKHGHRRYFVLKLETADAPARLEYYENARKFRHSVRAAAAAAAAAASGAAVPALIPPRRVITLYQCFSVSQRADARYRHLIALFTQDEYFAMVAENESEQESWYLLLSRLILESKRRRCGTPGAQPDGEPAALAAAAAAEPPFYKDVWQVVVKPRGLGHRKELSGVFRLCLTDEEVVFVRLNTEVASVVVQLLSIRRCGHSEQYFFLEVGRSTVIGPGELWMQVDDCVVAQNMHELFLEKMRALCADEYRARCRSYSVSIGAHLLTLLSARRHLDVLPLEPSGWLRRSRFEQFCHLRAIGDGEDEMLLTRRYITPSEPVHPSRRGRLYVPRARRSRRAVSVPASCFRHLAPNPVRAPRPAEARNLGTCLSPEASGSGSGNSGEEGNPEGKEGQEGNGGDYMPMNNWGSGNGRGSGGGQGSSGQGSSSQGSDGNQCSGGGQGSGGGQGSSGQGAGGNQCSGDGQGTTGGHGSGGGQGAGGGHGSGSGQGPGDGHGSGGGKNSGGGKGSGSGKGSDGNGERGKSLKKRSYFGKLTQSKQQQMPPPPPPPPPPPPAGATGGKGKSGGRFRLYFCADRGATKERKEAKEVKDVESPEDAARGPHRVRAFDEDEDDPYVPMRPGVAAPLASSSDYMPMAPQNVSASEKRHSRSPFEDSRGYMMMFPRVSPPSAPNPPKAPDPEKEDDSKDNDSDSDYMFMAPGAGAIPKNPRNPQGGSSSKSWSSYFSLPNPLQSSPLGQSDHSEYVPMLPGKLLGKGLDKEASSQGDPKDAASKPSVKQSFSKPGDGGSPSKPSDESPPKIKAKRPNRLSFITKGNKIKPKPQKPTREQREADSSSGYVNVDFTKRDSNTPAPSTQGLPGLWGIIADPRRSVFSNYVNVEFGVPFPNPGNDLSHLLRAIPGANPLFLDGARWPLLPLPPSATGSNANEEEGDYIEVMFNPAMIPAVPFADRAIRYDAETGRIYVVDPFSECCMDISLSPSRCSEPPPVARLLLEEELERRRPQSRSQGFFASTRAAVSAFPTDSLERDLSASLASAAALAAAPTLGVGRALAAASALAAAPGIGAAASGFEAAAGFDSASVRWFQPVANAADAEAVMGAQDVASGSNPRAQNPSSDLDGDEDGTGEAAAAAAVPPTPPRRQMPRPPEQEDSDDDDDTYVRMDFARSDNKKFDSPRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K464</accession>
    <name>A0A9L0K464_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Usherin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="26" type="primary">USH2A</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="17">Interacts with collagen IV and fibronectin via its laminin EGF-like domains. Interaction with collagen may be required for stable integration into the basement membrane. Interacts with NINL. Interacts with USH1C. Component of USH2 complex, composed of ADGRV1, PDZD7, USH2A and WHRN. Interacts with ADGRV1/MASS1 (via N-terminal PDZ domain). Interacts (via the cytoplasmic region) with WHRN. Interacts (via the cytoplasmic region) with PDZD7. Interacts (via the cytoplasmic region) with VEZT and MYO7A (via MyTH4-FERM domains); the interaction associates VEZT with the USH2 complex at the stereocilia base.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Cell projection</location>
            <location evidence="16">Stereocilium membrane</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005049264.1">
        <property type="protein sequence ID" value="ENSEASP00005060286.1"/>
        <property type="gene ID" value="ENSEASG00005003467.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158456"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005604">
        <property type="term" value="C:basement membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990075">
        <property type="term" value="C:periciliary membrane compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032391">
        <property type="term" value="C:photoreceptor connecting cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002142">
        <property type="term" value="C:stereocilia ankle link complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060171">
        <property type="term" value="C:stereocilium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990696">
        <property type="term" value="C:USH2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051649">
        <property type="term" value="P:establishment of localization in cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045184">
        <property type="term" value="P:establishment of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042491">
        <property type="term" value="P:inner ear auditory receptor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045494">
        <property type="term" value="P:photoreceptor cell maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007601">
        <property type="term" value="P:visual perception"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00055">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="31"/>
    </dbReference>
    <dbReference type="CDD" id="cd00110">
        <property type="entry name" value="LamG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000090">
        <property type="entry name" value="laminin subunit alpha"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000094">
        <property type="entry name" value="Laminin subunit alpha-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000224">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000275">
        <property type="entry name" value="usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000313">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.10.25.10:FF:000330">
        <property type="entry name" value="usherin"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000376">
        <property type="entry name" value="usherin"/>
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    <dbReference type="FunFam" id="2.10.25.10:FF:000412">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000111">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000125">
        <property type="entry name" value="Usherin"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000126">
        <property type="entry name" value="usherin"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000069">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000819">
        <property type="entry name" value="Usherin"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000915">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000991">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001004">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001023">
        <property type="entry name" value="usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001030">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001037">
        <property type="entry name" value="Usherin"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001052">
        <property type="entry name" value="Usherin"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001085">
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    <dbReference type="FunFam" id="2.60.40.10:FF:001099">
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    <dbReference type="FunFam" id="2.60.40.10:FF:001100">
        <property type="entry name" value="Usherin"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001135">
        <property type="entry name" value="Usherin"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001161">
        <property type="entry name" value="Usherin"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001168">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001172">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001173">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001176">
        <property type="entry name" value="Usherin"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:001201">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001211">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:001227">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001236">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001251">
        <property type="entry name" value="usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001255">
        <property type="entry name" value="usherin"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001276">
        <property type="entry name" value="Usherin"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001285">
        <property type="entry name" value="Usherin"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001296">
        <property type="entry name" value="Usherin"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001379">
        <property type="entry name" value="Usherin"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001390">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001416">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001716">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001882">
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001945">
        <property type="entry name" value="Usherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="33"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006558">
        <property type="entry name" value="LamG-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001791">
        <property type="entry name" value="Laminin_G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008211">
        <property type="entry name" value="Laminin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF7">
        <property type="entry name" value="USHERIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="10"/>
    </dbReference>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6 7">Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-methyl-5'-thioadenosine + phosphate = 5-(methylsulfanyl)-alpha-D-ribose 1-phosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:11852"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="EC" id="2.4.2.28"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.28" evidence="7"/>
    <dbReference type="RefSeq" id="XP_014686705.1">
        <property type="nucleotide sequence ID" value="XM_014831219.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K4A6"/>
    <dbReference type="Ensembl" id="ENSEAST00005056535.1">
        <property type="protein sequence ID" value="ENSEASP00005060189.1"/>
        <property type="gene ID" value="ENSEASG00005030405.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106824742"/>
    <dbReference type="KEGG" id="eai:106824742"/>
    <dbReference type="GeneTree" id="ENSGT00950000182991"/>
    <dbReference type="OrthoDB" id="431409at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09010">
        <property type="entry name" value="MTAP_SsMTAPII_like_MTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1580:FF:000006">
        <property type="entry name" value="Purine nucleoside phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01963">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018099">
        <property type="entry name" value="Purine_phosphorylase-2_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01694">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679:SF2">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01240">
        <property type="entry name" value="PNP_MTAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4 7" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="8">
        <location>
            <begin position="11"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="60"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="93"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="196"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="220"/>
            <end position="222"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="7">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="7">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00057208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005060189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="283" mass="31169" checksum="2A30CE7C3B672797" modified="2023-09-13" version="1">MAPGANPATVKIGIIGGTGLDDSEILEGRTEKYVDTPFGKPSDALILGKIKNVDCILLARHGRQHSIMPSEVNYRANIWALKEEGCTHVIVTTACGSLREEIRPGDIVIIDQFIDRTTKRPQTFYDGTSPCARGVCHIPAADPFCPKTRQVLLENAKKLGLRCHSKGTMVTIEGPRFSSRAESFMFRAWGADVINMTTVPEAVLAKEAGICYASIALATDYDCWKENEEEVSVDRVLKILKENGNKAKSLLLSTIPQIGSMEWSETLRTLKHMAQFSVLLPKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K4E1</accession>
    <name>A0A9L0K4E1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Calcium homeostasis modulator family member 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">CALHM6</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CALHM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K4E1"/>
    <dbReference type="Ensembl" id="ENSEAST00005045200.1">
        <property type="protein sequence ID" value="ENSEASP00005057217.1"/>
        <property type="gene ID" value="ENSEASG00005008375.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106847033"/>
    <dbReference type="KEGG" id="eai:106847033"/>
    <dbReference type="GeneTree" id="ENSGT01030000234610"/>
    <dbReference type="OMA" id="KFWKIYS"/>
    <dbReference type="OrthoDB" id="5962981at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904669">
        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029569">
        <property type="entry name" value="CALHM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32261">
        <property type="entry name" value="CALCIUM HOMEOSTASIS MODULATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32261:SF4">
        <property type="entry name" value="CALCIUM HOMEOSTASIS MODULATOR PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14798">
        <property type="entry name" value="Ca_hom_mod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="312"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="336"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005057217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="348" mass="37658" checksum="B388B7F72E07C3E8" modified="2023-09-13" version="1">MATTSWGSAIARFGTVLATYLKHHSKLGCGLASLLTASGELIFSTVVFQCPCSASWNLPYSLVFLLLPALVFYLLGCVLRTRSLCLLCSCCRTRNAGINRCDLCDECCRVSGPALVSSVTWVAVALLGGAVYECCASGSTFKADRLCVGRSSSCAAQLPLVPCRQAQDPLVQDLQKVLRAESQVVGWSLIAAVILLFLIFACVSHHRAGGSSLQLKFWKIYSEEEHQCLRTQVTERATKLADENVRCFFEGSRPTGCNIPSMECWQEISSPYPFNPEDEYLSALHRSVNEIQNRHTMNSPSGDEMPWGNVSSASGDAMPLGNVSSPSGGEVVRTLCSDSSNVEGTDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K6Z9</accession>
    <name>A0A9L0K6Z9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zinc finger protein 574</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ZNF574</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
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                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
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                <person name="Y."/>
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                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
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                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014867566.3"/>
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        <property type="nucleotide sequence ID" value="XM_014867567.3"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_044760309.2"/>
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    <dbReference type="RefSeq" id="XP_044616246.1">
        <property type="nucleotide sequence ID" value="XM_044760311.2"/>
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        <property type="nucleotide sequence ID" value="XM_070499115.1"/>
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    <dbReference type="RefSeq" id="XP_070355217.1">
        <property type="nucleotide sequence ID" value="XM_070499116.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070355218.1">
        <property type="nucleotide sequence ID" value="XM_070499117.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070355219.1">
        <property type="nucleotide sequence ID" value="XM_070499118.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070355220.1">
        <property type="nucleotide sequence ID" value="XM_070499119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K6Z9"/>
    <dbReference type="Ensembl" id="ENSEAST00005039245.1">
        <property type="protein sequence ID" value="ENSEASP00005046859.1"/>
        <property type="gene ID" value="ENSEASG00005031171.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005041934.1">
        <property type="protein sequence ID" value="ENSEASP00005054723.1"/>
        <property type="gene ID" value="ENSEASG00005031171.1"/>
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    <dbReference type="Ensembl" id="ENSEAST00005060108.1">
        <property type="protein sequence ID" value="ENSEASP00005053620.1"/>
        <property type="gene ID" value="ENSEASG00005031171.1"/>
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    <dbReference type="Ensembl" id="ENSEAST00005075664.1">
        <property type="protein sequence ID" value="ENSEASP00005057095.1"/>
        <property type="gene ID" value="ENSEASG00005031171.1"/>
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    <dbReference type="Ensembl" id="ENSEAST00005082928.1">
        <property type="protein sequence ID" value="ENSEASP00005043391.1"/>
        <property type="gene ID" value="ENSEASG00005031171.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106847964"/>
    <dbReference type="CTD" id="64763"/>
    <dbReference type="GeneTree" id="ENSGT00940000161799"/>
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        <property type="component" value="Chromosome 26"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="zinc finger protein 574"/>
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        <property type="entry name" value="Zinc finger protein 574"/>
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        <property type="entry name" value="zinc finger protein 574"/>
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        <property type="entry name" value="Zinc finger protein 574"/>
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        <property type="entry name" value="zinc finger protein 574"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="13"/>
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        <property type="entry name" value="Znf_C2H2_sf"/>
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    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
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    <dbReference type="PANTHER" id="PTHR24393:SF158">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
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    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="6"/>
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        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="20"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="17">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
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            <begin position="239"/>
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    <feature type="region of interest" description="Disordered" evidence="16">
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            <begin position="433"/>
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    <feature type="region of interest" description="Disordered" evidence="16">
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            <begin position="687"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
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            <begin position="274"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
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            <begin position="433"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="16">
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            <begin position="707"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
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            <dbReference type="ARBA" id="ARBA00022481"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068598"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005057095.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="896" mass="98985" checksum="8747EBD1EE81E36F" modified="2023-09-13" version="1">MTEESEETVLYIEHRYVCSECNQLYGSLEEVLMHQNSHVPQQHFELVGVADPGVTVATEAASGTGLYQTIVQESQYQCLECGQLLMSPSQLLEHQELHLKMMAPQEAVPAEPPPKAPPLSSSTIHYECVDCKALFASQELWLNHRQTHLRATPTKPQAPVVLGSPVVLGPPVSQARVAVEHSYRKAEEGGEGAVVPSAAATTTEVVTEVELLLYKCSECSQLFQLPADFLEHQATHFPAAAPESEEPALQQETLNPSPAEVPVSQPDPLPSSDHSYELRNGEAIGRDRRGRKARKNNNGEPGGAATQELFCSACDQLFLSPHQLQQHLRSHREGIFKCPLCSRVFPSPSSLDQHLGDHSSESHFLCVDCGLAFGTEALLLAHRRAHTPNPLHSCPCGKTFVNLTKFLYHRRTHGVGGVPLPTTPVPPEEPVIGFPEPAPAETGEPEAPEPPTSEESSAGPAAPGTYRCLLCSREFGKALQLTRHQRFVHRLERRHKCGICGKMFKKKSHVRNHLRTHTGERPFPCPDCSKPFNSPANLARHRLTHTGERPYRCGDCGKAFTQSSTLRQHRLVHAQHFPYRCQECGVRFHRPYRLLMHRYHHTGEYPYKCRECPRSFLLRRLLEVHQLVAHAGRQPHRCSSCGAAFPSSLRLREHRCAAAAAQAPRRFECGTCGKKVGSAARLQAHEAAHAAAGPGEVLAKEPPAPRAPRATRTPVVSPTTLGGTATAAPAAPARRRGLECSECKKLFSTETSLQVHRRIHTGERPYPCPDCGKAFRQSTHLKDHRRLHTGERPFACEVCGKAFAISMRLAEHRRIHTGERPYSCPDCGKSYRSFSNLWKHRKTHQQQHQAAVRQQLAEAEAAVGLAVMETAVEALPLVEAIEIYPLAEAEGVQISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K8X8</accession>
    <name>A0A9L0K8X8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cationic amino acid transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106826204</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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            <location evidence="1">Membrane</location>
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                <person name="Zhang"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Guo"/>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="Zhuang"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </evidence>
    <sequence length="311" mass="35511" checksum="59C9FF80B4A4EA51" modified="2023-09-13" version="1">MCWITLKFEHYTTGDVALKTIILSLFGIGAPANVVLFFHNVSPILLGQRQRPTHTILTHMAMANVLVLLCTGIPHMMTAFVLRKPLPSLGCKLVYYIRQVARSTSLCSTCVLSTYQFFTLVPERVMWMMLRGRAPKFIGPFCCNCWMFSFLINIYIPVKVTGPQDMGNDTDTQGKWFCSSSNPNASIVILWSVSDAMFIGLMIWASGSMVLLLHRHHQRVQHIHTRNTYQKYSPETRAAYTILTLVVTFVIFYMLNSIFTFYITAFLDSRLWLMQTSSVLVSCFPTLSPFLLIIRDPTTPRFCSLIVRYRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KA85</accession>
    <name>A0A9L0KA85_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Protein phosphatase 1 regulatory subunit 16A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">PPP1R16A</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Inhibits protein phosphatase 1 activity toward phosphorylase, myosin light chain and myosin substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Binds PP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044636846.1">
        <property type="nucleotide sequence ID" value="XM_044780911.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0KA85"/>
    <dbReference type="Ensembl" id="ENSEAST00005040279.1">
        <property type="protein sequence ID" value="ENSEASP00005061743.1"/>
        <property type="gene ID" value="ENSEASG00005038542.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106841891"/>
    <dbReference type="KEGG" id="eai:106841891"/>
    <dbReference type="CTD" id="84988"/>
    <dbReference type="GeneTree" id="ENSGT00940000154090"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004857">
        <property type="term" value="F:enzyme inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017020">
        <property type="term" value="F:myosin phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000198">
        <property type="entry name" value="Myosin binding subunit, isoform P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000079">
        <property type="entry name" value="Protein phosphatase 1 regulatory subunit 16B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017417">
        <property type="entry name" value="Pase-1_reg_su_16AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051226">
        <property type="entry name" value="PP1_Regulatory_Subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24179">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24179:SF30">
        <property type="entry name" value="PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038159">
        <property type="entry name" value="PP1_16AB_vert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 16" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="propeptide" id="PRO_5041033291" description="Removed in mature form" evidence="15">
        <location>
            <begin position="524"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="16">
        <location>
            <begin position="103"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="16">
        <location>
            <begin position="136"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="16">
        <location>
            <begin position="231"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="16">
        <location>
            <begin position="264"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="36"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="367"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="460"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="382"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="516"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="modified residue" description="Cysteine methyl ester" evidence="15">
        <location>
            <position position="523"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="S-palmitoyl cysteine" evidence="15">
        <location>
            <position position="522"/>
        </location>
    </feature>
    <feature type="lipid moiety-binding region" description="S-farnesyl cysteine" evidence="15">
        <location>
            <position position="523"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023289"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00055219"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00063230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00072668"/>
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    </evidence>
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            <dbReference type="PIRSR" id="PIRSR038159-50"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="526" mass="57764" checksum="526A342DEC762C18" modified="2023-09-13" version="1">MAEHLELLAEMPMVGRMSTQERLKHAQKRRAQQVKMWAQAEKEAQGKKGHRERLRKEAASDRPQKRVLFPPSVTLLEAAARNDLEEVRYFLESGVSPDLANEDGLTALHQSCIDDFREMVQQLLEAGAKVNARDSESWTPLHAAATCGHLHLVELLIARGADLLAVNTDGNMPYDLCEDEQTLDCLETAMANRGITQDSIEGARALPELHMLDDIRSLLQAGADLDAPLDHGATLLHIAAANGFSEAAALLLEHRASLSAKDHDGWEPLHAAAYWGQVHLVELLVAHGADLNGKSLMDETPFDVCGDEEVRAKLLELKHKHDALLRSQGRQRSLLHRRTSSAGSRGKVVRRVSLTQRTSLYRKEHAQEAIVWQQPPPASPEPPEEDEDDGLTDVELGPPPPQEEDPEVARPHNGRVGAPSGRHLYSKRLDRSVSYQLSALESTAHDALVQAKAHHTLAELKRQRAAAKLQRSPPEGPEAPESGLPADPENPQSECGSSAGEDPPLLKLTAPSEEAPVEKRPCCLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KBD0</accession>
    <name>A0A9L0KBD0_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">C-C motif chemokine ligand 21</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CCL21</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the intercrine beta (chemokine CC) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KBD0"/>
    <dbReference type="Ensembl" id="ENSEAST00005041143.1">
        <property type="protein sequence ID" value="ENSEASP00005059732.1"/>
        <property type="gene ID" value="ENSEASG00005035409.1"/>
    </dbReference>
    <dbReference type="KEGG" id="eai:106841408"/>
    <dbReference type="GeneTree" id="ENSGT01130000278316"/>
    <dbReference type="OMA" id="CKRTEQP"/>
    <dbReference type="OrthoDB" id="9445745at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:CCR chemokine receptor binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:chemokine activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0061844">
        <property type="term" value="P:antimicrobial humoral immune response mediated by antimicrobial peptide"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:chemokine-mediated signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0048245">
        <property type="term" value="P:eosinophil chemotaxis"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="C-C motif chemokine 21"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Chemokine_b/g/d"/>
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    <dbReference type="InterPro" id="IPR001811">
        <property type="entry name" value="Chemokine_IL8-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036048">
        <property type="entry name" value="Interleukin_8-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015:SF72">
        <property type="entry name" value="C-C MOTIF CHEMOKINE 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12015">
        <property type="entry name" value="SMALL INDUCIBLE CYTOKINE A"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="IL8"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SCY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54117">
        <property type="entry name" value="Interleukin 8-like chemokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="4" id="KW-0202">Cytokine</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040425267" evidence="10">
        <location>
            <begin position="24"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Chemokine interleukin-8-like" evidence="11">
        <location>
            <begin position="28"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="89"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010868"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    <evidence type="ECO:0000259" key="11">
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005059732.1"/>
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    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="131" mass="14351" checksum="F39AD5302B7E6CF6" modified="2023-09-13" version="1">MAQSLALSLLVLVLAFCIPWTQGSDGGAQDCCLRYSQRKIPPKAVRSYRKQEPSLGCAIPAVVFSPRKRSQPELCADPKEAWVQQLMQRLDKPPARQKPVQDCKKDKGGSKSGKKGKGTKGCKRTETPKGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KBG8</accession>
    <name>A0A9L0KBG8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Argininosuccinate lyase</fullName>
            <ecNumber evidence="4">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ASL</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the reversible cleavage of L-argininosuccinate to fumarate and L-arginine, an intermediate step reaction in the urea cycle mostly providing for hepatic nitrogen detoxification into excretable urea as well as de novo L-arginine synthesis in nonhepatic tissues. Essential regulator of intracellular and extracellular L-arginine pools. As part of citrulline-nitric oxide cycle, forms tissue-specific multiprotein complexes with argininosuccinate synthase ASS1, transport protein SLC7A1 and nitric oxide synthase NOS1, NOS2 or NOS3, allowing for cell-autonomous L-arginine synthesis while channeling extracellular L-arginine to nitric oxide synthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24021"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:24022"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nitrogen metabolism; urea cycle; L-arginine and fumarate from (N(omega)-L-arginino)succinate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homotetramer. Forms tissue-specific complexes with ASS1, SLC7A1, HSP90AA1 and nitric oxide synthase NOS1, NOS2 or NOS3; the complex maintenance is independent of ASL catalytic function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_014697781.1">
        <property type="nucleotide sequence ID" value="XM_014842295.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0KBG8"/>
    <dbReference type="Ensembl" id="ENSEAST00005051285.1">
        <property type="protein sequence ID" value="ENSEASP00005062720.1"/>
        <property type="gene ID" value="ENSEASG00005008837.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831856"/>
    <dbReference type="KEGG" id="eai:106831856"/>
    <dbReference type="CTD" id="435"/>
    <dbReference type="GeneTree" id="ENSGT00950000183122"/>
    <dbReference type="OrthoDB" id="2561043at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019676">
        <property type="term" value="P:ammonia assimilation cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007626">
        <property type="term" value="P:locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045429">
        <property type="term" value="P:positive regulation of nitric oxide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000001">
        <property type="entry name" value="Argininosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000015">
        <property type="entry name" value="argininosuccinate lyase isoform X2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.10:FF:000014">
        <property type="entry name" value="Os03g0824900 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020557">
        <property type="entry name" value="Fumarate_lyase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814:SF1">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00163">
        <property type="entry name" value="FUMARATE_LYASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0835">Urea cycle</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="14">
        <location>
            <begin position="11"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="domain" description="Argininosuccinate lyase C-terminal" evidence="15">
        <location>
            <begin position="368"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF14698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005062720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="464" mass="51859" checksum="A8822DA97AC2ED2B" modified="2023-09-13" version="1">MASESGKLWGGRFVGTVDPIMEKFNSSITYDRHLWEVDVQGSKAYSRGLEKAGLLTKAEVDEILHGLDKVAEEWAQDTFKLNPNDEDIHTANERRLKELIGETAGKLHTGRSRNDQVVTDLRLWMRQNCWTLSALLWGLIRTMVDRAEAERDILFPGYTHLQRAQPIRWSHWILSHAVALTRDSERLLEVQKRINVLPLGSGAIAGNPLGVDRELLRAELNFGAITLNSMDATSERDFVAEFLFWASLCMTHLSRMAEDLILYSTKEFSFVQLSDAYSTGSSLMPQKKNPDSLELIRSKAGRVFGRCAGLLMTLKGLPSTYNKDLQEDKEAVFEVSDTMSAVLQVATGIISTLQIHPENMARALSPDMLATDLAYYLVRKGMPFRQAHEASGKAVFMAETKGVALNQLSLQELQTISPLFSGDVSHVWDYGHSVEQYAALGGTARSSVDWQISQVRALLRAQQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KIV0</accession>
    <name>A0A9L0KIV0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">PAK6</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="RefSeq" id="XP_014702830.1">
        <property type="nucleotide sequence ID" value="XM_014847344.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014702831.1">
        <property type="nucleotide sequence ID" value="XM_014847345.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044621101.1">
        <property type="nucleotide sequence ID" value="XM_044765166.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0KIV0"/>
    <dbReference type="Ensembl" id="ENSEAST00005044608.1">
        <property type="protein sequence ID" value="ENSEASP00005061585.1"/>
        <property type="gene ID" value="ENSEASG00005016572.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835170"/>
    <dbReference type="KEGG" id="eai:106835170"/>
    <dbReference type="CTD" id="56924"/>
    <dbReference type="GeneTree" id="ENSGT00940000156528"/>
    <dbReference type="OMA" id="ARRQTMW"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007612">
        <property type="term" value="P:learning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007626">
        <property type="term" value="P:locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007613">
        <property type="term" value="P:memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140058">
        <property type="term" value="P:neuron projection arborization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990138">
        <property type="term" value="P:neuron projection extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01093">
        <property type="entry name" value="CRIB_PAK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06659">
        <property type="entry name" value="STKc_PAK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000073">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000141">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.810.10:FF:000002">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.810.10">
        <property type="entry name" value="CRIB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000095">
        <property type="entry name" value="CRIB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036936">
        <property type="entry name" value="CRIB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051931">
        <property type="entry name" value="PAK3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033923">
        <property type="entry name" value="PAK_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035066">
        <property type="entry name" value="STKc_PAK6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832:SF3">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45832">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE SAMKA-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00786">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00285">
        <property type="entry name" value="PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50108">
        <property type="entry name" value="CRIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CRIB" evidence="14">
        <location>
            <begin position="12"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="406"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="132"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="199"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="268"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="201"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="271"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="319"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="435"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="680" mass="74492" checksum="CE467ACCA3A8DBE5" modified="2023-09-13" version="1">MFRKKKKKRPEISAPQNFQHRVHTSFDPKEGKFVGLPPQWQNILDTLRRPKPVVDPSHITRVQLQPMKTVVRGSSVPSDGYISGLLSDIQKLSVISSNTLRGRSPTSRRRAQSLGLLGDEHWATDPDMYLQSPQSEHTDPHGLYLSCNGGTPAGRRQTPWPEPQSPRVLPNGLATKAQSLGPAEFQGATQRCLQLGACLQSSPPGTSPPTATGRRGAKAARHGSEEARPQSCLVGSAAGRPGGEGSPSPKTQENSLKRRLFRSMFLSTPATAPPSSKPGPPLQSKPSSSFRPPQKGSPTSLVAKAQSLPSDQPVGIFSPLTTSDTSSPQKSLRTAPATGPPPGRSSPAGSPRTRHAQISTSNLYLPHDPTVAKGALAGEDTGVVTHEQFKAALRMVVDQGDPRLLLDSYVKIGEGSTGIVCLAREKHSGRQVAVKMMDLRKQQRRELLFNEVVIMRDYQHVNVVEMYKSYLVGEELWVLMEFLQGGALTDIVSQVRLNEEQIATVCEAVLQALAYLHAQGVIHRDIKSDSILLTLDGRVKLSDFGFCAQISKDVPKRKSLVGTPYWMAPEVISRSLYATEVDIWSLGIMVIEMVDGEPPYFSDSPVQAMKRLRDSPPPKLKNSHKVSPVLRDFLERMLVRDPQERATAQELLDHPFLLQTGLPECLVPLIQLYRKQTSAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KT99</accession>
    <name>A0A8C4KT99_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mediator of RNA polymerase II transcription subunit 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MED7</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014698188.1">
        <property type="nucleotide sequence ID" value="XM_014842702.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014698189.1">
        <property type="nucleotide sequence ID" value="XM_014842703.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014698190.1">
        <property type="nucleotide sequence ID" value="XM_014842704.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044633674.1">
        <property type="nucleotide sequence ID" value="XM_044777739.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070373259.1">
        <property type="nucleotide sequence ID" value="XM_070517158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KT99"/>
    <dbReference type="Ensembl" id="ENSEAST00005001285.2">
        <property type="protein sequence ID" value="ENSEASP00005001127.1"/>
        <property type="gene ID" value="ENSEASG00005000944.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005063910.1">
        <property type="protein sequence ID" value="ENSEASP00005060057.1"/>
        <property type="gene ID" value="ENSEASG00005000944.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106832125"/>
    <dbReference type="KEGG" id="eai:106832125"/>
    <dbReference type="CTD" id="9443"/>
    <dbReference type="GeneTree" id="ENSGT00940000154444"/>
    <dbReference type="OMA" id="IHDSYSM"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035019">
        <property type="term" value="P:somatic stem cell population maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051669">
        <property type="entry name" value="Immune_Mod/Transcr_Coactivator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037212">
        <property type="entry name" value="Med7/Med21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009244">
        <property type="entry name" value="Mediatior_Med7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044888">
        <property type="entry name" value="Mediatior_Med7_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15498:SF72">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15498">
        <property type="entry name" value="T-CELL IMMUNOGLOBULIN AND MUCIN DOMAIN CONTAINING TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05983">
        <property type="entry name" value="Med7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140718">
        <property type="entry name" value="Mediator hinge subcomplex-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0010">Activator</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="178"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="178"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="202"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU364060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="233" mass="27191" checksum="A973A2BA9DD7A139" modified="2022-01-19" version="1">MGEPQQVSALPPPPMQYIKEYTDENIQEGLAPKPPPPIKDSYMMFGNQFQCDDLIIRPLESQGIERLHPMQFDHKKELRKLNMSILINFLDLLDILIRSPGSIKREEKLEDLKLLFVHVHHLINEYRPHQARETLRVMMEVQKRQRLETAERFQKHLERVIEMIQSCLASLPDDLPHSEGGIRVKTEPMDADDSNNCSGQNERQRENSGHRRDQIIEKDAALCVLIDEMNERP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KVV5</accession>
    <name>A0A8C4KVV5_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Protein tyrosine phosphatase receptor type N2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">PTPRN2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the protein-tyrosine phosphatase family. Receptor class 8 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KVV5"/>
    <dbReference type="Ensembl" id="ENSEAST00005001932.2">
        <property type="protein sequence ID" value="ENSEASP00005001735.2"/>
        <property type="gene ID" value="ENSEASG00005001394.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154095"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030141">
        <property type="term" value="C:secretory granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030658">
        <property type="term" value="C:transport vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035773">
        <property type="term" value="P:insulin secretion involved in cellular response to glucose stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051046">
        <property type="term" value="P:regulation of secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14610">
        <property type="entry name" value="R-PTP-N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000017">
        <property type="entry name" value="receptor-type tyrosine-protein phosphatase-like N isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2470">
        <property type="entry name" value="Protein-tyrosine phosphatase receptor IA-2 ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033522">
        <property type="entry name" value="IA-2/IA-2_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021613">
        <property type="entry name" value="Receptor_IA-2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038112">
        <property type="entry name" value="Receptor_IA-2_ectodomain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029403">
        <property type="entry name" value="RESP18_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46106">
        <property type="entry name" value="IA-2 PROTEIN TYROSINE PHOSPHATASE, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46106:SF5">
        <property type="entry name" value="RECEPTOR-TYPE TYROSINE-PROTEIN PHOSPHATASE N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11548">
        <property type="entry name" value="Receptor_IA-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14948">
        <property type="entry name" value="RESP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01305">
        <property type="entry name" value="RESP18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="557"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="15">
        <location>
            <begin position="686"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="16">
        <location>
            <begin position="865"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="59"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="617"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="67"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="629"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="646"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001735.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="956" mass="105817" checksum="CB6C781164679D5A" modified="2023-09-13" version="2">MDAVFGRCQKVPVIDIYRYEVSPVVLQHLTATLQKLSHTGFTWQDDYTQHVMAQELSNLPKTYPQRPESSNPARTSMQSVDNEKRYSQEGDIALAKALQRYLPYLEALSQATTSNLLPRMKHGRPPSEGEDALAESVLTYVAQTSALTYAPMPREDYPAGHPLRTLSRLQPDELSPKVVGSVDRQNLVAALGAYAAQKHPPPPREGDPGLHNLLHAPWREPRVLSAPATPQKWPLSPGDPKHPLGRGDEALIQSLLKDLRRHQVDMASLSPLDPEEMARVIANAMQGVGTEGEREEAGMGVGGEPGEQVDSPEAGLHEARKVGDVRDNRVQDDDDRVYEEVNRLSLTLGDLLQDPGSQFLPGAPPLVESFKTEIKKSEDPGASLSSEEESAGVENVRSQTYVKELLERPQVDGFGEFQRQIPGAWKEDRRLEAGAQGRSGEGLELEVQPSEESGYIVTETNPLSLEKGKELLAGVARLLEVPMSVFVDIDVVGPAVTFKVSANVLNVTTAEAVEAAVENKDNLEKTSGLKILQTGVGSKSKLKLRPHQAEQEDSTKFIVLTLISVAAILGVLLASGVIYCLRHSSHYRLKEKLSGPGGHAGLDATAYQELCRQHMAVRTSDRPEAPHTSRISSVSSQFSDGPMPSPSARSSTSSWSEEPAQPNMDISTGHMVLAYMEDHLKNKNRLEKEWEALCAYQAEPNSSLVAQREENMPKNRCPAVLTYDHSRILLKSENSHSNSDYINASPIMDHDPRNPAYIATQGPLPATVADFWQMVWESGCVVIVMLTPLSENGVRQCYHYWPDEGSNLYHIYEVNLVSEHIWCEDFLVRSFYLKNLQTNETRTVTQFHFLSWYDQGVPSSTRSLLDFRRKVNKCYRGRSCPIIVHCSDGAGRSGTYVLIDMVLNKMAKGAKEIDIAATLEHLRDQRPGMVQTKEQFEFALTAVAEEVNAILKALPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KYY0</accession>
    <name>A0A8C4KYY0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Myeloperoxidase</fullName>
            <ecNumber evidence="18">1.11.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">MPO</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>chloride + H2O2 + H(+) = hypochlorous acid + H2O</text>
            <dbReference type="Rhea" id="RHEA:28218"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:24757"/>
            <dbReference type="EC" id="1.11.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homodimer; disulfide-linked. Each monomer consists of a light and a heavy chain. Found in a complex with CP and LTF; interacts directly with CP, which protects CP antioxidant properties by MPO.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Lysosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.11.2.2" evidence="18"/>
    <dbReference type="RefSeq" id="XP_014682466.2">
        <property type="nucleotide sequence ID" value="XM_014826980.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014682476.2">
        <property type="nucleotide sequence ID" value="XM_014826990.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KYY0"/>
    <dbReference type="GlyCosmos" id="A0A8C4KYY0">
        <property type="glycosylation" value="6 sites, No reported glycans"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005004065.2">
        <property type="protein sequence ID" value="ENSEASP00005003706.2"/>
        <property type="gene ID" value="ENSEASG00005002803.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822013"/>
    <dbReference type="KEGG" id="eai:106822013"/>
    <dbReference type="CTD" id="4353"/>
    <dbReference type="GeneTree" id="ENSGT00940000161343"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042582">
        <property type="term" value="C:azurophil granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050832">
        <property type="term" value="P:defense response to fungus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002149">
        <property type="term" value="P:hypochlorous acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034374">
        <property type="term" value="P:low-density lipoprotein particle remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002679">
        <property type="term" value="P:respiratory burst involved in defense response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001878">
        <property type="term" value="P:response to yeast"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd09824">
        <property type="entry name" value="myeloperoxidase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.640.10:FF:000030">
        <property type="entry name" value="Myeloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.640.10">
        <property type="entry name" value="Haem peroxidase domain superfamily, animal type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019791">
        <property type="entry name" value="Haem_peroxidase_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010255">
        <property type="entry name" value="Haem_peroxidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037120">
        <property type="entry name" value="Haem_peroxidase_sf_animal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475:SF108">
        <property type="entry name" value="MYELOPEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11475">
        <property type="entry name" value="OXIDASE/PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03098">
        <property type="entry name" value="An_peroxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00457">
        <property type="entry name" value="ANPEROXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48113">
        <property type="entry name" value="Heme-dependent peroxidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50292">
        <property type="entry name" value="PEROXIDASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 20" id="KW-0349">Heme</keyword>
    <keyword evidence="15" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="10 20" id="KW-0408">Iron</keyword>
    <keyword evidence="14" id="KW-0458">Lysosome</keyword>
    <keyword evidence="6 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0558">Oxidation</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 21" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="21">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040200631" description="Myeloperoxidase" evidence="21">
        <location>
            <begin position="23"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="20">
        <location>
            <position position="476"/>
        </location>
        <ligand>
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00062550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00066993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00074280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR619791-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003706.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="724" mass="81963" checksum="662313ACA99C8A52" modified="2023-09-13" version="2">MKLLLALAGLLAIVAVPQPSEGATPAVLGEVDTSVVLTCMEEAKRLVDKAYKERRESIKQRLRSGSASPMELLSYFKQPVAATRTAVRAADYLHVALGLLERKLRSLWSGPFNVTDVLTPAQLNLLSKSSGCAHQNVGVRCPEKDKYRTISGQCNNRRSPTLGASNRPFARWLPAEYEDGFSLPFGWTPGVNRSGFPVPLARAVSNAIVRFPTEQLTPDRERSLIFMQWGQLLDHDLDLSPEPAARVSFITGVNCETSCLQQPPCFPLKIPPNDPRIQNQRDCIPFFRSSPACTENNITIRNQINALTSFVDASMVYGSEDPVAMKLRNLTNQLGLLAINTQFSDKGRALLPFDTLHDDPCLLTNRSVRIPCFLAGDTRSSEMPELTSMHTLFLREHNRLATQLKRLNPRWNGERLYQEARKIVGAMVQIITYRDYLPLVLGPEAMRKYLPRYRSYNDSVDPRIANVFTNAFRYGHTLIQPFMFRLDNQYRPMQPNPRVPLSRVFFATWRVVLEGGIDPILRGLMATPAKLNRQNQIVVDELRERLFEQVMRIGLDLPALNMQRSRDHGLPGYNAWRRFCGLPQPSTVGELGTVMKNMDLARKLMEQYRTPDNIDIWMGGVAEPLEPKGRVGKLLACLIGTQFRKLRDGDRFWWENKGVFSTQQKRALAKVSLPRIICDNTGITTVSKNNIFMSKTFPRDFVNCSTLPTLDLSSWRERDWRLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KZN9</accession>
    <name>A0A8C4KZN9_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Proline and serine rich coiled-coil 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PSRC1</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KZN9"/>
    <dbReference type="Ensembl" id="ENSEAST00005004028.2">
        <property type="protein sequence ID" value="ENSEASP00005003669.2"/>
        <property type="gene ID" value="ENSEASG00005002792.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154189"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001578">
        <property type="term" value="P:microtubule bundle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007080">
        <property type="term" value="P:mitotic metaphase chromosome alignment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030308">
        <property type="term" value="P:negative regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031116">
        <property type="term" value="P:positive regulation of microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060236">
        <property type="term" value="P:regulation of mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026657">
        <property type="entry name" value="DDA3/GTSE-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032768">
        <property type="entry name" value="GTSE1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21584">
        <property type="entry name" value="DIFFERENTIAL DISPLAY AND ACTIVATED BY P53 DDA3 /G2 S PHASE EXPRESSED 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21584:SF1">
        <property type="entry name" value="PROLINE_SERINE-RICH COILED-COIL PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15259">
        <property type="entry name" value="GTSE1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G2 and S phase-expressed protein 1 N-terminal" evidence="6">
        <location>
            <begin position="36"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="64"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="121"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="139"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="159"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="203"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF15259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003669.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="353" mass="37323" checksum="3D73C08456635099" modified="2023-09-13" version="2">MGVQEGLPPPPPELTGLSYLLLPAGWNMGDLEEDVKFIVDETLDLGGLSPSDSREEEDIAVLMTPEKPLRRGLSHRSDPNAVAPAPQGLRLSLGPLSPEKLEEILDEANRLAAQLEQCALQEREITGEGPGPRRVKPSPRRETFVLKDSPVRDLLPTVSSLARSTPSPSSLTPRLRSSDRKGSVRALRAASGKRPSSVKKESPTCNLFPTAKSPASSPLARSTPPVRGKAGPSGRAIASPPTPVRPALAPQPSTSNSQRLSRPQGAAVKPSSRLPVPSAIPRPASRMPLASRSVPPSRGALPPDSQSTRKGLPRPSAAGQRVPISQRPNLPVTGAPRSNLQPPRKAAVPGPTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L0T9</accession>
    <name>A0A8C4L0T9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Paracaspase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">MALT1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Protease that enhances BCL10-induced activation: acts via formation of CBM complexes that channel adaptive and innate immune signaling downstream of CARD domain-containing proteins (CARD9, CARD11 and CARD14) to activate NF-kappa-B and MAP kinase p38 pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines. Mediates BCL10 cleavage: MALT1-dependent BCL10 cleavage plays an important role in T-cell antigen receptor-induced integrin adhesion. Involved in the induction of T helper 17 cells (Th17) differentiation. Cleaves RC3H1 and ZC3H12A in response to T-cell receptor (TCR) stimulation which releases their cooperatively repressed targets to promote Th17 cell differentiation. Also mediates cleavage of N4BP1 in T-cells following TCR-mediated activation, leading to N4BP1 inactivation. May also have ubiquitin ligase activity: binds to TRAF6, inducing TRAF6 oligomerization and activation of its ligase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Homooligomer; forms oligomers which bind to TRAF6. Forms a complex with CARD14 and MALT1; resulting in the formation of a CBM (CARD14-BCL10-MALT1) complex. Forms a complex with CARD11 and MALT1; resulting in the formation of a CBM (CARD11-BCL10-MALT1) complex. Forms a complex with CARD9 and MALT1; resulting in the formation of a CBM (CARD9-BCL10-MALT1) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase C14B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L0T9"/>
    <dbReference type="Ensembl" id="ENSEAST00005003863.2">
        <property type="protein sequence ID" value="ENSEASP00005003529.2"/>
        <property type="gene ID" value="ENSEASG00005002711.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018044"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032449">
        <property type="term" value="C:CBM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002096">
        <property type="term" value="C:polkadots"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061133">
        <property type="term" value="F:endopeptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019209">
        <property type="term" value="F:kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001923">
        <property type="term" value="P:B-1 B cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
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        <property type="entry name" value="Death Domain, Fas"/>
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    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
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        <location>
            <begin position="125"/>
            <end position="201"/>
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            <end position="422"/>
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            <end position="23"/>
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    <sequence length="828" mass="92597" checksum="0A410854089FCD86" modified="2023-09-13" version="2">TSLCGELLQAPPPPAAPSGPPLSAPAGATLNRLPEPLLRRLSGSLDRAPEGRGWRRLAEVAASRGRLRLSGLDLEQCSLKVLEPEGSPSLCLLKLMGEKGCTVTDLSDFLQAMDHTEVLQLLSPPGIKITVNPESKAVLAGQFVKLCCRATGHPFVQYQWFKMNKEIPNGNASELIFNTVHVKDAGFYVCRVNNHFTFEFSQWSQLDVCDVTEVTESFRGSLDGVSESKLQICVEPRSQKLMPGSTLVLQCVAVGSPIPQYQWFKNESPLTHETKKLYMVPYVDLEHQGTYWCHVYNDRDSQHSKKVEIIIGRTDEAVECTEDELNHLGHPDNKEQTADQPLAKDKVALLIGNMNYWEHPKLKAPLVDVYELTNLLRQLDFKVVSLLDLTEYEMRNAVDEFLLLLDKGVYGLLYYAGHGYENFGNSFMVPVDAPNPYRSENCLCVQNILKLMQEKETGLNVFLLDMCRKRNDYDDTIPILDALKVTANIVFGYATCQGAEAFEIQHSGLANGIFMKFLKDRLLEDKKITVLLDEVAEDMGKCHLTKGKQALEIRSSLSEKRALTDPIQGTAYSAESLVRNLQWAKAHELPESMCLKFQCGVQIQLGFAAEFSNVMIIYTSIVHKPPDIMMCDAYVTDFPLDLDIDPKDANKGTPEETGSYLVSKDLPKHCLYTRLSSLQKLKEHLIFTVCLSYQYSGLEDTVEEKQEVNVGKPLIAKLDIHRGLGRKTCFQTCIMCNGPYQSSTATSAEAGRYHSSQDSFHGVYHSHLGNSSNILPSDSCRCSRTADAFLSSHHTHQYSCQFGRSNMPVETTDEIPFSFSDGLRISEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L4F2</accession>
    <name>A0A8C4L4F2_EQUAS</name>
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        <submittedName>
            <fullName evidence="10">Nucleic acid binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">NABP2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the SOSS complex, a multiprotein complex that functions downstream of the MRN complex to promote DNA repair and G2/M checkpoint. In the SOSS complex, acts as a sensor of single-stranded DNA that binds to single-stranded DNA, in particular to polypyrimidines. The SOSS complex associates with DNA lesions and influences diverse endpoints in the cellular DNA damage response including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. Required for efficient homologous recombination-dependent repair of double-strand breaks (DSBs) and ATM-dependent signaling pathways.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SOSS-B family. SOSS-B1 subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014843885.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014699372.1">
        <property type="nucleotide sequence ID" value="XM_014843886.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014699374.1">
        <property type="nucleotide sequence ID" value="XM_014843888.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070350766.1">
        <property type="nucleotide sequence ID" value="XM_070494665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L4F2"/>
    <dbReference type="Ensembl" id="ENSEAST00005007265.2">
        <property type="protein sequence ID" value="ENSEASP00005006644.1"/>
        <property type="gene ID" value="ENSEASG00005004905.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106832948"/>
    <dbReference type="KEGG" id="eai:106832948"/>
    <dbReference type="CTD" id="79035"/>
    <dbReference type="GeneTree" id="ENSGT00940000161079"/>
    <dbReference type="OMA" id="QSKAAQN"/>
    <dbReference type="OrthoDB" id="295715at2759"/>
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        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070876">
        <property type="term" value="C:SOSS complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070182">
        <property type="term" value="F:DNA polymerase binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:G-rich strand telomeric DNA binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:double-strand break repair via homologous recombination"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:establishment of protein localization to telomere"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:mitotic G2/M transition checkpoint"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of telomere capping"/>
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        <property type="term" value="P:response to ionizing radiation"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
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        <property type="entry name" value="OB FOLD NUCLEIC ACID BINDING PROTEIN-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="SOSS COMPLEX SUBUNIT B1"/>
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    <dbReference type="Pfam" id="PF21473">
        <property type="entry name" value="OB_Ssb-like"/>
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    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Single-stranded DNA binding protein Ssb-like OB fold" evidence="9">
        <location>
            <begin position="11"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="110"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="115"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
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            <begin position="158"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="211" mass="22352" checksum="B7AFC151ADBC003A" modified="2022-01-19" version="1">MTTETFVKDIKPGLKNLNLIFIVLETGRVTKTKDGHEVRTCKVADKTGSINISVWDDVGNLIQPGDIIRLTKGYASVFKGCLTLYTGRGGDLQKIGEFCMVYSEVPNFSEPNPEYSAQQAPNKTVQNDSSPTAPQPTTGPPATSPASESQNGNGLSTPPGPGGGPHPPHTPSHPPSTRITRSQPNHTAAGPPGPSSNPVSNGKETRRSSKR</sequence>
</entry>
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    <accession>A0A8C4L5C8</accession>
    <name>A0A8C4L5C8_EQUAS</name>
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        <recommendedName>
            <fullName evidence="18">Cytochrome P450 family 2 subfamily B member 6</fullName>
        </recommendedName>
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        <name evidence="16" type="primary">LOC106843260</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the cytochrome P450 family.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSEASP00005006566.2"/>
        <property type="gene ID" value="ENSEASG00005004834.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157162"/>
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        <property type="component" value="Chromosome 26"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008392">
        <property type="term" value="F:arachidonate epoxygenase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019373">
        <property type="term" value="P:epoxygenase P450 pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000001">
        <property type="entry name" value="Cytochrome P450, family 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008068">
        <property type="entry name" value="Cyt_P450_E_grp-I_CYP2B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF406">
        <property type="entry name" value="CYTOCHROME P450 2B6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01685">
        <property type="entry name" value="EP450ICYP2B"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 13 14" id="KW-0349">Heme</keyword>
    <keyword evidence="10 13 14" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0492">Microsome</keyword>
    <keyword evidence="11 14" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="9 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040156436" description="Cytochrome P450 family 2 subfamily B member 6" evidence="15">
        <location>
            <begin position="25"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="13">
        <location>
            <position position="436"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006566.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="511" mass="57941" checksum="5BD668352D8ACA58" modified="2023-09-13" version="2">MVLSVFLLLALLTGLLLLLPRGHTKTHSYLPRGPHPLPFFGNLLQMDRRGLLKSFLRLQEKYGDVFTVYLGPRPVVMLCGTEAIREALVDQAEAFSGRGKIAIIGQVIQGYGVIFSNGERWKTLRKFSLATMRDFGMGKRSVEEQIQEEAQCLVEELQNTKGALQDSIFFFHASAANIICSIVFGKRFSYRDPQFLQLLDLFHQCFVLRSSFFGQLFELFSDFLKHFPGIHRQVYKNLMEIKAFTGCSVKEHRETLDPSNPLDFIDTYLLCIDKVGFGRGHEFHEQNLMHTVLSLFFAGMETVSITLHYGFLLMLKHPHITERVHKEIDLVIDSHHPPALNDRAKMPYTDAVIHEIQRFSDLIPLGIPHVVTKDTHFRGYIIPKDPQVYPILSSALHDPRYFEKPDAFYPDHFLDANGALKKNEAFIPFSTGKRTCLGEGTARTELFLFFTTILQNFSIASPVAPEDIDLTPRESGVGRIPPAYQISFLPPQGGWGMGTEGFWGHEVAPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L8V8</accession>
    <name>A0A8C4L8V8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Glutamine rich 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">QRICH2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
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        <text evidence="10">Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.</text>
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        <property type="entry name" value="Calpain_III_sf"/>
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    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF322">
        <property type="entry name" value="CALPAIN-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01067">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00720">
        <property type="entry name" value="calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 14" id="KW-0645">Protease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 14" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="15">
        <location>
            <begin position="42"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="16">
        <location>
            <begin position="601"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="active site" evidence="13 14">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="active site" evidence="13 14">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <feature type="active site" evidence="13 14">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00063791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005004875.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="700" mass="80433" checksum="460F6008133C9760" modified="2023-09-13" version="2">MAAHISQSRLKAKGVGRYNNAQSYSNQIFEELRAVCLRKGELFEDSLFPAEHSSLGFKDLGPDSKNAQNIRWQRPTQITSNPQFIVDGVGPTDICQGVLGDCWLLAAIGSLTTYPKLLNRVVPKGQSFKKNYAGIFHFQIWQFGQWVDVVVDDRLPTKNGKLVFVHSPERTEFWSALLEKAYAKLNGSYEALSGGNTVEGFEDFTGGVTQSLQLQRPPQNLLRMLRKAIERSSLLGCSIEITSNSDLESLTQKMLVRGHAYSVTGLQDVLYQGKMETLIRVRNPWGRIEWNGAWSDNAREWEEVDLDIQKQLLHKKEDGEFWMSYQDFLNNFTLLEICNLMPDTLSGDCKSSWHATFYEGSWRRGSTAGGCRNYLDTFWTNPQFKISLPEEDDDPENNEVVCTCLVALMQKNWRQARPQGAQLQTIGFVIYTVGAPFQNIQDIHLKKDFFMKYQDHGFSEIFSNAREVSSHLRLPPGDYIIIPSTFEPHKDADFLLRVFTEKHSESWELDEANYAEMLQEENISEDKVNQDFTRLFETVAGGEGKEIGMYGLQKLLNKVVSKFTHFKTQGFGLDVCRCMVNLMDKDDSGKLGFLEFQILWKKIKKWTDIFRECDEDNSGALNSYEMRLAIEKAGIKLNNKVMQVLVARYANENMMVDFDSFIYCFLKLKAMFRYFLTMDPRNTGYIHLNLNEWLQITMWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LAW6</accession>
    <name>A0A8C4LAW6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Vitamin D 25-hydroxylase</fullName>
            <ecNumber evidence="21">1.14.14.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Cytochrome P450 2R1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">LOC106830430</name>
        <name evidence="26" type="synonym">CYP2R1</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">A cytochrome P450 monooxygenase involved in activation of vitamin D precursors. Catalyzes hydroxylation at C-25 of both forms of vitamin D, vitamin D(2) and D(3) (calciol). Can metabolize vitamin D analogs/prodrugs 1alpha-hydroxyvitamin D(2) (doxercalciferol) and 1alpha-hydroxyvitamin D(3) (alfacalcidol) forming 25-hydroxy derivatives. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>1alpha-hydroxyvitamin D2 + reduced [NADPH--hemoprotein reductase] + O2 = 1alpha,25-dihydroxyvitamin D2 + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46584"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:4712"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:86320"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:46585"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>alfacalcidol + reduced [NADPH--hemoprotein reductase] + O2 = calcitriol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:49272"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17823"/>
            <dbReference type="ChEBI" id="CHEBI:31186"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:49273"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>calciol + reduced [NADPH--hemoprotein reductase] + O2 = calcidiol + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32903"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:17933"/>
            <dbReference type="ChEBI" id="CHEBI:28940"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="1.14.14.24"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:32904"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>vitamin D2 + reduced [NADPH--hemoprotein reductase] + O2 = 25-hydroxyvitamin D2 + oxidized [NADPH--hemoprotein reductase] + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46580"/>
            <dbReference type="Rhea" id="RHEA-COMP:11964"/>
            <dbReference type="Rhea" id="RHEA-COMP:11965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:28934"/>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="ChEBI" id="CHEBI:86319"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:46581"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 24">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="20">Hormone biosynthesis; vitamin D biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Microsome membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 25">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.14.24" evidence="21"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4LAW6"/>
    <dbReference type="Ensembl" id="ENSEAST00005009070.2">
        <property type="protein sequence ID" value="ENSEASP00005008322.2"/>
        <property type="gene ID" value="ENSEASG00005006005.2"/>
    </dbReference>
    <dbReference type="KEGG" id="eai:106830430"/>
    <dbReference type="GeneTree" id="ENSGT00940000158305"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016712">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030343">
        <property type="term" value="F:vitamin D3 25-hydroxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006805">
        <property type="term" value="P:xenobiotic metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20661">
        <property type="entry name" value="CYP2R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000030">
        <property type="entry name" value="vitamin D 25-hydroxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050182">
        <property type="entry name" value="Cytochrome_P450_fam2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300">
        <property type="entry name" value="CYTOCHROME P450 508A4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24300:SF48">
        <property type="entry name" value="VITAMIN D 25-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 24 25" id="KW-0349">Heme</keyword>
    <keyword evidence="11 24 25" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 24 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0492">Microsome</keyword>
    <keyword evidence="12 25" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="10 25" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="24">
        <location>
            <position position="448"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00059364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00060543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00066438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00069698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00083017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008322.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="501" mass="57123" checksum="09CC0FA806B20191" modified="2023-09-13" version="2">MWNPRGAEACAAALGGALLLLLFALGVRQLLKQRRPAGFPPGPSGLPFIGNIYSLAASAELPHVYMRKQSQAYGEIFSLDLGGISTVVLNGYDVVKECLVHQSEIFADRPCLPLFKKMTKMGGLLNARYGRGWVDHRRLAVNSFRYFGYGQKSFESKILEETKFFIDAVETYKGRPFDFKQLITNAVSNITNLIIFGERFTYEDTDFQHMIELFSENVELAASASVFLYNAFPWIGILPFGKLQQLFRNADVVYEFLSRLIEKVSVNRKPQLPQHFVDAYLDEMDQGQNDPSSTFSKENLIFSVGELIIAGTETTTNVLRWAILFMALYPNIQGQVQKEIDLIMGPSGKPSWDDKCKMPYTEAVLHEVLRFCNIVPLGIFHATSEDTVVRGYSIPKGTTVITNLYSVHFDEKYWRDPEIFYPERFLDSSGYFAKKEAFIPFSLGRRHCLGEQLARMEMFLFFTALLQRFHLHFPHELVPNLKPRLGMTLQPQAYLICAERR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LBF0</accession>
    <name>A0A8C4LBF0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">10-formyltetrahydrofolate dehydrogenase</fullName>
            <ecNumber evidence="9">1.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ALDH1L1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(6R)-10-formyltetrahydrofolate + NADP(+) + H2O = (6S)-5,6,7,8-tetrahydrofolate + CO2 + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10180"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="1.5.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:10181"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">In the N-terminal section; belongs to the GART family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.6" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4LBF0"/>
    <dbReference type="SMR" id="A0A8C4LBF0"/>
    <dbReference type="Ensembl" id="ENSEAST00005009337.2">
        <property type="protein sequence ID" value="ENSEASP00005008563.1"/>
        <property type="gene ID" value="ENSEASG00005006155.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106834458"/>
    <dbReference type="KEGG" id="eai:106834458"/>
    <dbReference type="CTD" id="10840"/>
    <dbReference type="GeneTree" id="ENSGT00940000160913"/>
    <dbReference type="OMA" id="FENGVWG"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016155">
        <property type="term" value="F:formyltetrahydrofolate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016620">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009258">
        <property type="term" value="P:10-formyltetrahydrofolate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07140">
        <property type="entry name" value="ALDH_F1L_FTFDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08703">
        <property type="entry name" value="FDH_Hydrolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08647">
        <property type="entry name" value="FMT_core_FDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.10:FF:000002">
        <property type="entry name" value="10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.25.10:FF:000002">
        <property type="entry name" value="10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.170:FF:000002">
        <property type="entry name" value="10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000026">
        <property type="entry name" value="Aldehyde dehydrogenase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000008">
        <property type="entry name" value="Cytosolic 10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000009">
        <property type="entry name" value="Cytosolic 10-formyltetrahydrofolate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.10">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.25.10">
        <property type="entry name" value="Formyl transferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011407">
        <property type="entry name" value="10_FTHF_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036736">
        <property type="entry name" value="ACP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005793">
        <property type="entry name" value="Formyl_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037022">
        <property type="entry name" value="Formyl_trans_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002376">
        <property type="entry name" value="Formyl_transf_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036477">
        <property type="entry name" value="Formyl_transf_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011034">
        <property type="entry name" value="Formyl_transferase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001555">
        <property type="entry name" value="GART_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009081">
        <property type="entry name" value="PP-bd_ACP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02911">
        <property type="entry name" value="Formyl_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00551">
        <property type="entry name" value="Formyl_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00550">
        <property type="entry name" value="PP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036489">
        <property type="entry name" value="10-FTHFDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47336">
        <property type="entry name" value="ACP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50486">
        <property type="entry name" value="FMT C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53328">
        <property type="entry name" value="Formyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50075">
        <property type="entry name" value="CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00373">
        <property type="entry name" value="GART"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 12" id="KW-0521">NADP</keyword>
    <keyword evidence="5 9" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7 9 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0596">Phosphopantetheine</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carrier" evidence="16">
        <location>
            <begin position="318"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="673"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="673"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="707"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="88"/>
            <end position="90"/>
        </location>
        <ligand>
            <name>(6R)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>(6R)-10-formyltetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="571"/>
            <end position="573"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="597"/>
            <end position="600"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="630"/>
            <end position="635"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="650"/>
            <end position="651"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="757"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="804"/>
            <end position="806"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Essential for catalytic activity" evidence="13">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR036489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036489-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036489-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR036489-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR036489-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="902" mass="99007" checksum="7DF9A79A0D50C981" modified="2022-01-19" version="1">MKIAVIGQSLFGQEVYCHLRKEGHEVVGVFTVPDKDGKADPLGLEAEKDGVPVFKFPRWRTKGQALPDVVAKYQALGAELNVLPFCSQFIPMEIINAPCHGSIIYHPSLLPRHRGASAINWTLIHGDKKGGFSIFWADDGLDTGDLLLQKECEVLPDDTVSSLYNRFLFPEGVKGMVQAVRLIAEGRAPRLPQPEEGATYEGIQKKETAKINWEQPAEAIHNWIRGNDKVPGAWTEACGQKLTFFNSTLNTVGLVPEGEALPIPGAHRPGLVTKDGLVLFGNDDRMLLVKNIQLEDGKMILASHFFKGADSSVLELTEAELVTAEAVRSAWKRILPNILEVEDSTDFFKSGAASVDVVRLVEEVKELCDGLELENEDVYMATTFRDFIQLLVRKLRGDDKESECVIDYVEKEANRLTIKMPHQLFIGGAFVDAEGDKTYETINPTDGSAICQVSLAQVSDVDKAVAAAKDAFENGLWGKISARDRGRLLYRLADLMEQHQEELATIEALDAGAVYTLALKTHVGMSIQTFRYFAGWCDKIQGSTIPINHARPNRNLSLTRKEPIGVCGIVIPWNYPLMMLSWKTAACLAAGNTVVIKPAQVTPLTALKFAELTLKAGIPKGVINILPGSGSLVGQRLSDHPDVRKIGFTGSTEVGKHIMKSCALSNVKKVSLELGGKSPLIIFADCDLSKAVQMGMSSVFFNKGENCIAAGRLFVEDSIHDQFVQKVVEEVRKMKIGNPLDRDTTHGPQNHQAHLRKLIEYCQRGVKEGATLVCGGNQVPRPGFFFEPTVFTDVEDHMFIAKEESFGPIMIISRFANGDVDTVLSRANATEFGLASGVFTKDINKALYVSDKLQAGTVFVNTYNKTDVAAPFGGFKQSGFGKDLGEAALNEYLRVKTVTFEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LBV1</accession>
    <name>A0A8C4LBV1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Solute carrier family 12 member 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">Na-Cl symporter</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Thiazide-sensitive sodium-chloride cotransporter</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">SLC12A3</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Electroneutral sodium and chloride ion cotransporter, which acts as a key mediator of sodium and chloride reabsorption in kidney distal convoluted tubules. Also acts as a receptor for the pro-inflammatory cytokine IL18, thereby contributing to IL18-induced cytokine production, including IFNG, IL6, IL18 and CCL2. May act either independently of IL18R1, or in a complex with IL18R1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>chloride(out) + Na(+)(out) = chloride(in) + Na(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:73887"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="21">Homodimer; adopts a domain-swap conformation at the scissor helices connecting the transmembrane domain and C-terminal domain. Interacts with KLHL3. Interacts with IL18R1; this interaction is increased by IL18 treatment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC12A transporter family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LBV1"/>
    <dbReference type="Ensembl" id="ENSEAST00005007524.2">
        <property type="protein sequence ID" value="ENSEASP00005006887.2"/>
        <property type="gene ID" value="ENSEASG00005004963.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155044"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008511">
        <property type="term" value="F:sodium:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055064">
        <property type="term" value="P:chloride ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055075">
        <property type="term" value="P:potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070294">
        <property type="term" value="P:renal sodium ion absorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904044">
        <property type="term" value="P:response to aldosterone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902074">
        <property type="term" value="P:response to salt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055078">
        <property type="term" value="P:sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000018">
        <property type="entry name" value="solute carrier family 12 member 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013612">
        <property type="entry name" value="AA_permease_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018491">
        <property type="entry name" value="SLC12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002948">
        <property type="entry name" value="SLC12A3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004842">
        <property type="entry name" value="SLC12A_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00930">
        <property type="entry name" value="2a30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827:SF9">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08403">
        <property type="entry name" value="AA_permease_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03522">
        <property type="entry name" value="SLC12"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01230">
        <property type="entry name" value="NACLTRNSPORT"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0868">Chloride</keyword>
    <keyword evidence="15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14 25" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0915">Sodium</keyword>
    <keyword evidence="17" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="10" id="KW-0769">Symport</keyword>
    <keyword evidence="6 25" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 25" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="168"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="214"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="259"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="284"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="339"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="452"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="505"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="531"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="25">
        <location>
            <begin position="571"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease N-terminal" evidence="28">
        <location>
            <begin position="46"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="26">
        <location>
            <begin position="140"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="27">
        <location>
            <begin position="654"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="27">
        <location>
            <begin position="825"/>
            <end position="1028"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00050884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00056815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00063035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00073714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00076232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00077939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF03522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF08403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006887.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1029" mass="113367" checksum="B40723EEBCF2F3CD" modified="2023-09-13" version="2">MAELPVSETPGDPALCSGRFTISTLLGGDEPPPPAAYDSSHPSHLTHGSTFHMRTFGYNTIDVVPAYDHYANSTLPGEPRKVRPTLADLHSFLKEGSHLHALAFDSRPSHEMTDGLVEDEAGTGGEKSPAEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLIQEHGAPIVDPINDIRIIGVVTVTVLLGISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRGDIFVQNLVPDWRGVDGSFFGMFSIFFPSATGILAGANISGDLRDPAVAIPKGTLMAIFWTTVSYLAISATIGSCVVRDASGDLNDTMTPGSGACEGLACGYGWNFTHCSQQHSCRYGLINYYQSMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCMDQLYPLIGFFGKGYGKNNEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAVISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSSSVGLNDVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLVGPRKQRMPELRLIANGHTKWLNKRKIKAFYSEVIAEDLRSGVQILMQAAGLGRMKPNILVVGFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNISEMMQAHINPVFDPAEDPKEAIARRARPSVSGTLDPEALVREEQASTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKKRWSKCAIRVFVGGQINRMDQERKAIVSLLSKFRLGFHEVHVLPDINQKPRAEHTKRFEDMIAPFRLNDGFKDEATVTEMRRDCPWKISDEEINKNRVKSLRQVRLNEILLDYSRDAALVVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFYCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LFX6</accession>
    <name>A0A8C4LFX6_EQUAS</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LFX6"/>
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        <property type="protein sequence ID" value="ENSEASP00005009233.2"/>
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    </dbReference>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
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        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="KAP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13885">
        <property type="entry name" value="Keratin_B2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009233.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="226" mass="23634" checksum="A6F80F4D41243E61" modified="2023-09-13" version="2">MTHSCCSPCCQPTCCRTTCCQTTCYQPACVTSCRPSCCSAPCCQPTCSESSCCGQTCSGSSCCQPCCPSTCCQTTCCQPAYSGPMYCRRTCYHPTCVCLGPSCGGQTCSGSSCCQPCCPQTRCQVTCCKTTCYQPTCVTSCCRPSCCSAPFCQPTCSESSCCGQTCSQSSCCQPCCPSTCCQTICCQPACSGPVYCRRTCYHPTCVCLPCCQAQSCGSSCCQPSCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LGJ0</accession>
    <name>A0A8C4LGJ0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Endoplasmic reticulum-Golgi intermediate compartment protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ERGIC3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="G."/>
                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Plays a role in transport between endoplasmic reticulum and Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 8">Endoplasmic reticulum-Golgi intermediate compartment membrane</location>
            <topology evidence="2 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Golgi apparatus membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">cis-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the ERGIC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070341913.1">
        <property type="nucleotide sequence ID" value="XM_070485812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LGJ0"/>
    <dbReference type="SMR" id="A0A8C4LGJ0"/>
    <dbReference type="Ensembl" id="ENSEAST00005008387.2">
        <property type="protein sequence ID" value="ENSEASP00005007687.2"/>
        <property type="gene ID" value="ENSEASG00005005303.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837280"/>
    <dbReference type="CTD" id="51614"/>
    <dbReference type="GeneTree" id="ENSGT00530000063113"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045888">
        <property type="entry name" value="Erv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012936">
        <property type="entry name" value="Erv_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039542">
        <property type="entry name" value="Erv_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10984:SF25">
        <property type="entry name" value="ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07970">
        <property type="entry name" value="COPIIcoated_ERV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13850">
        <property type="entry name" value="ERGIC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter N-terminal" evidence="10">
        <location>
            <begin position="7"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="Endoplasmic reticulum vesicle transporter C-terminal" evidence="9">
        <location>
            <begin position="145"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU369013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007687.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="388" mass="43928" checksum="0CFB94C998D89043" modified="2023-09-13" version="2">MEALGKLKQFDAYPKTLEDFRVKTCGGATVTIVSGLLMLLLFLSELQYYLTTEVHPELYVDKSRGDKLKINIDVFFPHMPCAYLSIDAMDVAGEQQLDVEHNLFKQRLDKDGIPVSSEAERHELGKVEVKVFDPDSLDPDRCESCYGAETEDIKCCNTCEDVREAYRRRGWAFKNPDTIEQCRREGFSQKMQEQKNEGCQVYGFLEVNKVAGNFHFAPGKSFQQSHVHVHAVEIHDLQSFGLDNINMTHYIRHLSFGEDYPGIVNPLDRTNVTAPQASMMFQYFVKVVPTVYMKVDGEVLRTNQFSVTRHEKVANGLMGDQGLPGVFVLYELSPMMVKLTEKHRSFTHFLTGVCAIIGGMFTVAGLIDSLIYHSARAIQKKIDLGKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LHB2</accession>
    <name>A0A8C4LHB2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Parathyroid hormone-related protein</fullName>
            <shortName evidence="5">PTH-rP</shortName>
            <shortName evidence="5">PTHrP</shortName>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="5">Osteostatin</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="8" type="primary">PTHLH</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. Required for skeletal homeostasis. Promotes mammary mesenchyme differentiation and bud outgrowth by modulating mesenchymal cell responsiveness to BMPs. Up-regulates BMPR1A expression in the mammary mesenchyme and this increases the sensitivity of these cells to BMPs and allows them to respond to BMP4 in a paracrine and/or autocrine fashion. BMP4 signaling in the mesenchyme, in turn, triggers epithelial outgrowth and augments MSX2 expression, which causes the mammary mesenchyme to inhibit hair follicle formation within the nipple sheath.</text>
    </comment>
    <comment type="function">
        <molecule>Osteostatin</molecule>
        <text evidence="5">Potent inhibitor of osteoclastic bone resorption.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">PTHrP interacts with PTH1R (via N-terminal extracellular domain).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="5">There are several secretory forms, including osteostatin, arising from endoproteolytic cleavage of the initial translation product. Each of these secretory forms is believed to have one or more of its own receptors that mediates the normal paracrine, autocrine and endocrine actions.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the parathyroid hormone family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014702384.1">
        <property type="nucleotide sequence ID" value="XM_014846898.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014702387.1">
        <property type="nucleotide sequence ID" value="XM_014846901.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LHB2"/>
    <dbReference type="Ensembl" id="ENSEAST00005010092.2">
        <property type="protein sequence ID" value="ENSEASP00005009279.1"/>
        <property type="gene ID" value="ENSEASG00005006639.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106834903"/>
    <dbReference type="KEGG" id="eai:106834903"/>
    <dbReference type="CTD" id="5744"/>
    <dbReference type="GeneTree" id="ENSGT00390000004933"/>
    <dbReference type="OMA" id="TTHDHNL"/>
    <dbReference type="OrthoDB" id="9892514at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005179">
        <property type="term" value="F:hormone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051428">
        <property type="term" value="F:peptide hormone receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030282">
        <property type="term" value="P:bone mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061182">
        <property type="term" value="P:negative regulation of chondrocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001501">
        <property type="term" value="P:skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003626">
        <property type="entry name" value="PTH-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001415">
        <property type="entry name" value="PTH/PTH-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17223">
        <property type="entry name" value="PARATHYROID HORMONE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17223:SF0">
        <property type="entry name" value="PARATHYROID HORMONE-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01279">
        <property type="entry name" value="Parathyroid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00087">
        <property type="entry name" value="PTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00335">
        <property type="entry name" value="PARATHYROID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0372">Hormone</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5043848672" description="Parathyroid hormone-related protein" evidence="7">
        <location>
            <begin position="25"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="74"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="76"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU368098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="177" mass="20460" checksum="D551E66309DE0ECD" modified="2022-01-19" version="1">MLRRLVQQWSVAVFLLSYSVPSCGRSVEELGRRLKRAVSEHQLLHDKGKSIQDLRRRFFLHHLIAEIHTAEIRATSEVSPNSKPAPNTKNHPVRFGSDDEGRYLTQETNKLEPYKEQPLKTPGKKKKGKPGKRKEQEKKKRRTRSAWLNSEVAESGLDGDYLSDFSTTSPELYLRRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LHG7</accession>
    <name>A0A8C4LHG7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Wiskott-Aldrich syndrome protein family member</fullName>
            <shortName evidence="14">WASP family protein member</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">WASF1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes. Also involved in the regulation of mitochondrial dynamics.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Binds actin and the Arp2/3 complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Binds actin and the Arp2/3 complex. Interacts with BAIAP2. Interacts with SHANK3; the interaction mediates the association of SHANK3 with the WAVE1 complex. Interacts with ABI1 (via N-terminus). Interacts with SORBS2; this interaction greatly enhances phosphorylation by ABL1 and dephosphorylation by PTPN12 and might mediate partial to focal adhesion sites.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
            <location evidence="1 14">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the SCAR/WAVE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044613481.1">
        <property type="nucleotide sequence ID" value="XM_044757546.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044613483.1">
        <property type="nucleotide sequence ID" value="XM_044757548.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070352491.1">
        <property type="nucleotide sequence ID" value="XM_070496390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LHG7"/>
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</entry>
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    <name>A0A8C4LHZ3_EQUAS</name>
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        <submittedName>
            <fullName evidence="7">Membrane spanning 4-domains A13</fullName>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="2">Belongs to the MS4A family.</text>
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        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MEMBER 13"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="28"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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    <feature type="transmembrane region" description="Helical" evidence="6">
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        <location>
            <begin position="113"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011415.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="157" mass="17430" checksum="017DE7AF7AC5EEAD" modified="2023-09-13" version="2">MQIMLGIFHVFMWYFLFVLYMGQIKGVFGKYEPITYKTGCSLWGIIFIISGVSIIRATKYPNQRLITCALVTSILCIVTAIAATVITIMELVKFESVSYRNYGQAKLGREVSAILLSTYPLEVSIALAYSIFGCIGLSRGNEDTLSTVTIEEAESPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LKC8</accession>
    <name>A0A8C4LKC8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Vacuolar protein sorting 13 homolog C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">VPS13C</name>
    </gene>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the VPS13 family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005010103.2">
        <property type="protein sequence ID" value="ENSEASP00005009289.2"/>
        <property type="gene ID" value="ENSEASG00005006073.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183083"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032127">
        <property type="term" value="C:dense core granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006869">
        <property type="term" value="P:lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905090">
        <property type="term" value="P:negative regulation of type 2 mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045053">
        <property type="term" value="P:protein retention in Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006623">
        <property type="term" value="P:protein targeting to vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032868">
        <property type="term" value="P:response to insulin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026847">
        <property type="entry name" value="VPS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056748">
        <property type="entry name" value="VPS13-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056747">
        <property type="entry name" value="VPS13-like_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026854">
        <property type="entry name" value="VPS13_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009543">
        <property type="entry name" value="VPS13_VAB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166:SF125">
        <property type="entry name" value="INTERMEMBRANE LIPID TRANSFER PROTEIN VPS13C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16166">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25037">
        <property type="entry name" value="VPS13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25033">
        <property type="entry name" value="VPS13_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12624">
        <property type="entry name" value="VPS13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25036">
        <property type="entry name" value="VPS13_VAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Chorein N-terminal" evidence="5">
        <location>
            <begin position="28"/>
            <end position="1579"/>
        </location>
    </feature>
    <feature type="domain" description="VPS13-like middle region" evidence="6">
        <location>
            <begin position="1595"/>
            <end position="2392"/>
        </location>
    </feature>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 13 VPS13 adaptor binding" evidence="7">
        <location>
            <begin position="2467"/>
            <end position="3001"/>
        </location>
    </feature>
    <feature type="domain" description="Intermembrane lipid transfer protein VPS13-like C-terminal" evidence="8">
        <location>
            <begin position="3579"/>
            <end position="3684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="856"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1873"/>
            <end position="1908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="856"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1874"/>
            <end position="1884"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1887"/>
            <end position="1904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF25036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF25037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009289.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="3734" mass="419119" checksum="7AD9C8FEC2562F44" modified="2023-09-13" version="2">MRGRRSPPSAAPGGSGSTLGRRLSPTMVLESVVADLLNRFLGDYVENLNKSQLKLGIWGGDVALDNLQIKENALSELDVPFKVKAGQIDKLTLKIPWKNLYGEAVVATLEGLYLLVVPGASIKYDAEKEEKSLQDIKQKELSRIEEALQKAAEKDKPKEAKKDTFLEKLATQVIKNIQVKITDIHIKYEDDVTDPKQPLSFGVTLGELSLLTANKQWTPCILNEAEKIIYKLIRLDSLSAYWNVNCSVSYRRSREQILDQLKSEILTSSKIPPNHQYIFQPISASAKLYMNPYAETELKTPKLDWNIEVQNIAIELTKPQYLSMIDLLESVDYMVRNAPYRKYKPYLPLHTNGRQWWKYAIDSVLEVHIRRYTQMWSWSNIKKHRQLLKSYKNAYKNKLTQTKISEEIQKQIQDLEKTLDVFNIILARQQAQVEVIRSGQKLRKKSADTGEKRGGWFSGFWGKKESRKKDEESLIPETIDDLMTPEEKDKLFTAIGYSESTHDLTLPKQYVAHIMTLKLVSTSVTIRENKNIPEILKIQIIGLGTQVSQRPGAQALKVEAKLEHWYITGLRQQDIVPSLVASIGDTASSLLKIEFETNPENSTADQTLIVQSQPVEVIYDAKTINAVVEFFQSDKGLDLEQIKSATLMKLEEIKERTATGLTHIIETRKVLDLRINLKPSYLIVPQTGFHHKKSNLLILDFGTFQLNSKDQGLQKTSNSSLEEIMDKAYDKFDVEIKSVQLLFARAEENWKKCRFQHPSTMHILQPMDIHVELSKAMVEKDIRMARFKVSGGLPLMHVRISDKKMRDVLCLINSIPLPQISSAPSPERQVSSIPIISDRTKGLLGTSLLLDGVESESDDEYFDAEDGDTQPGKSKKGSELKKAAEAPNEELINLLLKFEIKEVVLEFTKQEKEENTILVFNVTQLGTEATVRTFDLTAVSYLKKISLDYHEIQGSKKKPLHLISSSDKPGLDLLKVEYIKADKNGPSFQTTFEKTEQTFKVAFSSLNLLMQTQALLSSINYLTTIIPSDGQNIGDTKEVQISTENQQKNSALQKVIVSSKDSDIIDFRLFAKLNAFCVIICDEKNNIAEIKIQGLDSSLSLQSRKQSLFARLENIVVTDIDPNTVHKKAVSIMGNEVFRFNLDLYPHATEGDAYTDMSKVDGVVSLNVGCIQIVYLHKFLMSLLNFLNNFQTAKEALSAATAQAAEKAATSVKDLAQRSFRVSINIDLKAPVIVIPQSSISTNAVVVDLGLIRVQNQFSLVSGEDYLNPPVIDRMDVQLTKLKLSRTVIQPGISQPDIQLLYPINLEFSVNRNLASSWYHKVPVVEIKGHLESMNVSLNQEDLNLLFRILAENLGEATEDLNKVKPGIQKTGEPKEPLETSVSQQDVRALQSTLTAGVEEIKSVDIINMVLNFEIKEVVITLMKKAEKKGRPIHELNVLQLGMEARVKTYDLTAQAYLKKISMRCFEFADSKGEPLHIINSSNVTDEPLLKMLLTKADSDGPEFKTIHENTKQRLKVSFSSLDLVLHLEALLSFMDFVSSAIPSSEPSYSEKESELKPLMGESRSIVVKAVSSSTSQDDVFDLKIIAELNAFNVFVCDQKCNIADIRIHGMDASISVKPKQTDMFARLKDIIVTNVDLLSIHKKAVSILGDEVFRFQMTLFPDATEGKAYADMSKVDGKLSLKVGCIQIVYVHKFFMSLLNFLNNFQTAKEALSAATVQAAERAASSMKDLAQKSFCLLMDINLKAPVIIIPQSSVSPNAVIADLGLIRVENKFSLVSVGHYSVPPVIDKMNIQLTQLKLSRTILQAGLPQHDIEILKPVNMLLSIQRNLAATWYTQIPGMEIKGKLKPMQVALSEDDLTVLMKILLENLGEASSQPSPTQSTQEAVRVRKDVSSGPDHLKAQEDLPDSELSVDQNVTLQFDFHFESLSIVLYNNDVNQESKLAFHNDNFRLGELRLHLMASAGKMFKDGSMNVSIKLKTCTLDDLREGIERATSRMIDRKNDQDNNSSMIDISYKQGKNGSHIDAVLDKLYICASVEFLMTVADFFIKAVSQSPENMAKEAQIPLRQTATAKLRIDKDDSLKPNMTLKAMITDPEVVFVASLTKADAPALTASFQCDLSLSTSKLEQMMEASVRDLKVLACPFLREKRGKNITTVLQPCSLFMEKCTWASGKQNINIIVEEFIIKISPIILNTVMTIMAALSPKTKEDESKDTSKETENLWGVKSISDYNSWFLGVDTATEITENFKDIDRPFIEENCAVVVESVQVTLECGLGHRTVPLLLAESKFSGNIKNWTSLMSAAADMTLEVHYYNEVHAVWEPLIERVEGKKQWNLRLDVKKNPVQDKSLMPGDDFGFIPEPQTAVHISSGDTMNITISKSCLNVFNNLAKGFSEGTASTFDYSLKDRAPFTVKNALGVPVKVQPNHNLRVMGSPEKSGIYDVDAGQNLELEYASVEPSRQGKLSILNRQESSFFTLTIVPHGYTEVGKIPVARPGRQLYNVRNPNATHSDSVLVQIDATEGNKVITIRSPLQIKNHFSVAFIIYKFVKNVKLLERIGVARPEEEFHVPLDSYRCQLFIQPAGILENQYKESTTYISWKEELHRSREVRCMLQCPSVEINFLPLMVNTVALPDELTYISTHGEDWDPAYIIHLYPTLTLRNLLPYSLRYLLEGAAETHELAEGSAADVLHSRISGEIMELVLMKYQGKNWNGHFRICDTLPEFFLVCFSSDSTEVMTLDLSVHVRRIGSRMVLSVFSPYWLINKTSRVLQYRSEDIHVKHPADLRDIILFSFKKKNIFSKNKVQLKISTSAWSSSFSLDTVGSYGCVKCPANNMEYLVGVSIKMSSFNLTRIVTLTPFCTIANKSSLELEVGEIASDGSMPTNKWNYIASSECLPFWPENLSGKLCVRVVGCEGSSKPFFYNRQDNGTLLSLEDLNGGILVDVNTAEHSTVITFSDYHEGSAPALIMNHTPCGILTYKQSGSQEEVELQPRQARLFAWADPTGTRKLTWKYAANVGEHDLLKDECGQFAYDANIQIHWVSFLDGRQRVLLFTDDVALVSKALQAEEMEQANHEITFSLHSLGLSLVNNENKQEVSYIGITSSGVIWEMKPKRKWKPFSQKQIILLEQSYKKYQESRGHGWIKLDNNFEVNFDKVPMEMRLPFQCPIKRDFLSGIQIEFKQSPHQRSLRARLYWLQVDNQLPGTMFPVLFHPVAPPKSIALDSEPKPFIDVSVITRFNEYSKVLQFKYFMVLIQEMALKVDQGFLGAITALFTPATDPEAESKRTKLIQQDVDALNTELMETSMTDMSSLSFFEHFHISPVKLHLSLSLSSGGEESDKEKQEMIAIHSVNLLLKSIGATLTDVDDLIFKLAYYEIKYQFYKRDQLMWSVFRHYTEQFLKQMYVLVLGLDVLGNPFGLIRGLSEGVEALFYEPFQGAVQGPEEFAEGLVIGVRSLFGHTVGGAAGVVSRITGSVGKGLAAITMDKEYQQKRREEMGRQPKDFGDSLARGGKGLLLGVVGGVTGIITKPVEGAKKEGAAGFFKGIGKGLVGAVARPTGGIIDMASSTFQGIQRAAESTEEVSSLRPPRLIHEDGIIRPYDRQESEGCDLFENHIKKLEGEIYRYHCAIPGNKKTILMVTNRRVLCIKEVEILGCMSIDWQCPFEDFVGPPSVSGNLLQILVKEQGLFHKKDSANQGYLRKIYLLDTTTAERACKAIEDAQSVRHQQKLVKQSSLKLLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LKH1</accession>
    <name>A0A8C4LKH1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Keratin 78</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">KRT78</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044611466.1">
        <property type="nucleotide sequence ID" value="XM_044755531.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LKH1"/>
    <dbReference type="Ensembl" id="ENSEAST00005014391.2">
        <property type="protein sequence ID" value="ENSEASP00005013247.1"/>
        <property type="gene ID" value="ENSEASG00005009254.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835261"/>
    <dbReference type="KEGG" id="eai:106835261"/>
    <dbReference type="CTD" id="196374"/>
    <dbReference type="GeneTree" id="ENSGT00940000162461"/>
    <dbReference type="OMA" id="ETQQIRT"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030280">
        <property type="term" value="F:structural constituent of skin epidermis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031424">
        <property type="term" value="P:keratinization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000004">
        <property type="entry name" value="Keratin, type II cytoskeletal 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032444">
        <property type="entry name" value="Keratin_2_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003054">
        <property type="entry name" value="Keratin_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616">
        <property type="entry name" value="GATA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616:SF18">
        <property type="entry name" value="KERATIN, TYPE II CYTOSKELETAL 78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16208">
        <property type="entry name" value="Keratin_2_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01276">
        <property type="entry name" value="TYPE2KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="8">
        <location>
            <begin position="105"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="17"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="153"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="309"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="17"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005013247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="514" mass="56174" checksum="4D1656A4CACBDC53" modified="2022-01-19" version="1">MSPSSCRAQRGFSARSACSARSGGRGRGSFSSRSLSSFRGCRGGSRGRDWGSGGRLGVRLGAGNGGPGLSLCPPGGIQEVTINQNLLTPLEIEINPQFQAVRTQETREIRALNNQFASFIDKVRYLEQQNKVLETKWHLLQQQQVSDSPKGLDSFFEAYLAQLRKQLEQLQRERGALDAELKSCQDQEEEYKTKYEQEAHKRATVENDFVVLKKDVDGVFLSKMELEGKLEAQREYICFLRHLYEEELGQLQTQDSDMSVVLSMDNNRCLDFSDVIAEIRARYEEIARTSKAEAEALYQTKYQELQASAQRHGDSMKETKVQVAQLQREIQRLQSQVENLKKQNANLQAAIADAEHRGELAVKDAQTKLEELEAALRMAKQDMARLLRDYQELMSTKLALDVEIATYRRLLEGEECRMSGECTSQITISVGGGSAVVSGGAGGGLVGTCGLGGGKGSFGSSCSSVVTGGSNVILSSGQGPVLGSGSVSGTSSSSSCHTILKKTVESSLKTSVTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LLS5</accession>
    <name>A0A8C4LLS5_EQUAS</name>
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        <recommendedName>
            <fullName evidence="13">Golgi-associated PDZ and coiled-coil motif-containing protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">CFTR-associated ligand</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">PDZ protein interacting specifically with TC10</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">GOPC</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Postsynaptic density</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014721612.2">
        <property type="nucleotide sequence ID" value="XM_014866126.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LLS5"/>
    <dbReference type="Ensembl" id="ENSEAST00005014232.2">
        <property type="protein sequence ID" value="ENSEASP00005013098.1"/>
        <property type="gene ID" value="ENSEASG00005009135.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106847019"/>
    <dbReference type="KEGG" id="eai:106847019"/>
    <dbReference type="CTD" id="57120"/>
    <dbReference type="GeneTree" id="ENSGT00940000156535"/>
    <dbReference type="OMA" id="QCNLRQE"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030140">
        <property type="term" value="C:trans-Golgi network transport vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140313">
        <property type="term" value="F:molecular sequestering activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000009">
        <property type="term" value="P:negative regulation of protein localization to cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06800">
        <property type="entry name" value="PDZ_GOPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000067">
        <property type="entry name" value="Golgi-associated PDZ and coiled-coil motif-containing protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038879">
        <property type="entry name" value="GOPC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16528">
        <property type="entry name" value="GOLGI-ASSOCIATED PDZ AND COILED-COIL MOTIF-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16528:SF2">
        <property type="entry name" value="GOLGI-ASSOCIATED PDZ AND COILED-COIL MOTIF-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PDZ" evidence="18">
        <location>
            <begin position="289"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="427"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="85"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="156"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="436"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
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    <sequence length="463" mass="50601" checksum="3F8762E2D37EF45E" modified="2022-01-19" version="1">MSTGGPCPAGAGGGPGGASCAVGVSAGGVSMFRWLEVLEKEFDKAFVDVDLLLGEIDPDQADITYEGRQKMTSLSSCFAQLCHKAQTVSQINHKLEAQLVDLKSELTETQAEKVVLEKEVHNQLLQLHSVQLQLHAKTGQSVDSGTIKAKLSVPSVEELERELEANKKEKMKEAQLEAEVKLLRKENEALRRHIAVLQAEVYGARLAAKYLDKELAGRVQQIQLLGRDMKGPAHDKLWNQLEAEIHLHRHKTVIRACRGRNDLKRPMQAPPGHDQDSLKKSQGVGPIRKVLLLKEDHEGLGISITGGKEHGVPILISEIHPGQPADRCGGLHVGDAILAVNGVNLRDTKHKEAVTILSQQRGEIEFEVVYVAPEVDSDDENVEYEDESGHRYRLYLDELEGGSNPGASCKDTSGEIKALQGFNKKAVTDGHENGDLGTSSETSLEDSASKLDDLHSVYQRKSS</sequence>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Zhao"/>
                <person name="Z."/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
                <person name="Q."/>
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                <person name="Guo"/>
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                <person name="Yang"/>
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                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
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                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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            <begin position="13"/>
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            <begin position="432"/>
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            <begin position="543"/>
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            <begin position="624"/>
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    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LP32</accession>
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        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Min"/>
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                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Enhances GTPase-activating protein (GAP) activity of regulator of G protein signaling (RGS) proteins, such as RGS7 and RGS9, hence involved in the termination of the signaling initiated by the G protein coupled receptors (GPCRs) by accelerating the GTP hydrolysis on the G-alpha subunits, thereby promoting their inactivation. Increases RGS7 GTPase-activating protein (GAP) activity, thereby regulating mood and cognition. Increases RGS9 GTPase-activating protein (GAP) activity, hence contributes to the deactivation of G protein signaling initiated by D(2) dopamine receptors. May play an important role in neuronal signaling, including in the parasympathetic, but not sympathetic, control of heart rate.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of a complex composed of RGS9 (isoform RGS9-1), GNB5 and RGS9BP; within this complex, the presence of GNB5 stabilizes both itself and RGS9 and increases RGS9 GTPase-activating protein (GAP) activity. Interacts with RGS7, forming the RGS7-GNB5 complex; within this complex, the presence of GNB5 increases RGS7 GTPase-activating protein (GAP) activity. Interacts with GPR158; promotes the GTPase activator activity of the RGS7-GNB5 complex in absence of glycine, in contrast GTPase activator activity of the RGS7-GNB5 complex is inhibited in presence of glycine. Interacts with RGS6.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat G protein beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044620813.1">
        <property type="nucleotide sequence ID" value="XM_044764878.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070357574.1">
        <property type="nucleotide sequence ID" value="XM_070501473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LP32"/>
    <dbReference type="SMR" id="A0A8C4LP32"/>
    <dbReference type="Ensembl" id="ENSEAST00005015596.2">
        <property type="protein sequence ID" value="ENSEASP00005014353.2"/>
        <property type="gene ID" value="ENSEASG00005009995.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837154"/>
    <dbReference type="KEGG" id="eai:106837154"/>
    <dbReference type="CTD" id="10681"/>
    <dbReference type="GeneTree" id="ENSGT01000000214413"/>
    <dbReference type="OMA" id="LDNKCTI"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000020">
        <property type="entry name" value="Guanine nucleotide-binding protein beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016346">
        <property type="entry name" value="G-protein_beta_1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19850">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN BETA G PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25391">
        <property type="entry name" value="WD40_Gbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002394">
        <property type="entry name" value="GN-bd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 10" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="59"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="149"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="191"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="235"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="288"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="10">
        <location>
            <begin position="321"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="9"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00041974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00042939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014353.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="353" mass="38746" checksum="1C9A2E71A4967384" modified="2023-09-13" version="2">MATDGLHENETLASLKSEAESLKGKLEEERAKLHDVELHQVAERVEALGQFVMKTRRTLKGHGNKVLCMDWCKDKRRIVSSSQDGKVIVWDSFTTNKEHAVTMPCTWVMACAYAPSGCAIACGGLDNKCSVYPLTFDKNENMAAKKKSVAMHTNYLSACSFTNSDMQILTASGDGTCALWDVESGQLLQSFHGHGADVLCLDLAPSETGNTFVSGGCDKKAMVWDMRSGQCVQAFETHESDINSVRYYPSGDAFASGSDDATCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLLFAGYNDYTINVWDVLKGSRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRVWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LSI9</accession>
    <name>A0A8C4LSI9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106825121</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="Ensembl" id="ENSEAST00005017036.2">
        <property type="protein sequence ID" value="ENSEASP00005015659.2"/>
        <property type="gene ID" value="ENSEASG00005031831.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155375"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome Y"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02659">
        <property type="entry name" value="peptidase_C19C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056850">
        <property type="entry name" value="ARM_UBP34_24_USP9X_Y"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021905">
        <property type="entry name" value="DUF3517"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055176">
        <property type="entry name" value="UBP24/USP9X/USP9Y_UBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF925">
        <property type="entry name" value="UBIQUITINYL HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25010">
        <property type="entry name" value="ARM_UBP24_USP9X-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12030">
        <property type="entry name" value="DUF3517"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22900">
        <property type="entry name" value="UCH_UBL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="10">
        <location>
            <begin position="1558"/>
            <end position="1957"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="966"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1595"/>
            <end position="1617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1841"/>
            <end position="1860"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="2478"/>
            <end position="2519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="969"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1602"/>
            <end position="1617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1842"/>
            <end position="1860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="2488"/>
            <end position="2506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005015659.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="2541" mass="289393" checksum="79A576CC2AB7E94B" modified="2023-09-13" version="2">MTATTRGSPVGGNDSQGQTPDGQTQPLFQQNQILSPDSSDENSPAAPSDEQGQGDAPPQHEGEEPAFPHTDLAKLDDMITRPRWVVPVLPKGELEVLLEAAIDLSKKGLDIKSEACQRFFRDGLTISFTKILMDEAVSGWKFEIHRCIINNTHRLIELCVAKLSQDWFPLLELLAMALNPHCKFHIYNGTRPCESISTSAQLPEDELFARSSDPRSPKGWLVDLINKFGTLNGFQILLDRFINGSALNVQIIAALIKPFGQCYEFLSQHTVKKYFIPVIEIVPRFLENLTDEELKKEAKNETKNDALSMIIKSLKNLASRIPGQEETVKNLEIFRLKMILRLLQISSFNGKMNALNEINKVISSVSYYTHRHGNPEEEDWLTAERMAEWIQQNNILSIVLRDSLHQPQYVEKLEKILRFVIKEKALTLQDLDNIWAAQAGKHEAIVKNVHDLLAKLAWDFSPEQLDHLFDCFKASWTNASKKQREKLLELIRRLAEDDKDGVMAHKVLNLLWNLAHSDDVPVDIMDLALSAHIKILDYSCSQDRDTQKVQWIDRFIEELRTNEKWVIPALKQIREICSLFGEAPQNLSQTQRSPHVFYRHDLINQLQHNHALVTLVAENLAAYMNSMRPYARDHEDYDPQTVRRGSRYSHVQEVQERLNFLRFLLKDGQLWLCAPQAKQIWKCLAENAVYLCDREACFKWYSKLMGDEPDLDPDINKDFFESNVLQLDPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMDDLELIGLDYLWRVVIQSNDDIASRAIDLLKEIYTNLGPRLRVNQVAIHEDFIQSCFDRLKASYDTLCVLDGDKDSMNCAIQEAVRMVRVLTVLREYISECDSDYHEERTILPMSRAFRGKHLSLIVRFPNHGRQVDDLDIWSHTNDTVGSVRRCILNRVKANVNHTKIELFVGGELIDSEDDRKLIGQLNLKDRSVITAKLTQISSNTPSSPDSSSDSSTGSPGNHCNHYSDGPNPEVESCLPGVIMSLHPRYISFLWQVADLGSSLNMPPLRDGARVLMKLMPPDSTTTEKLRAICLDHGKLGENNLGPSLDALFFGPSASRVLYLTEVVYALLMPAGAPLADDSSDFQFHFLKSGGLPLVLRMLTRNNFLPNTDTETRRGAYFNGLKIAKLLLTAVGYGHVRAVAEACQPVVDGADPITPINQVTHDQAVVLQNALQSIPNPSSECMLRNVSIRLAQQISDEASRYMPDTCVIRALQKIIWASGCGSLELVFSPNEEITKVYAVSSGASSEPDVEDEQVCCEALEVMTLCFALIPTALDTLSKEKTWQTFIIDLLLHCPSKTVRQLAQEQFFLMCTRCCMGHRPLLFFITLLFTILGNTARERGKHSGDYFTLLRHLLNYAYSSNINVPNAEILLNNEIDWLKRIRDNVKNTGETGVEEPNLEGHLGVTKELLAFQTPEKKYHIGCEKGGANLIKELIDDFIFPASKVYLQCMRNGELPPEQAIPVCSSPATINAGFELLVALAVGCVRNLKQIVDCLTEMYYIGTAITTCEALTEWEYLPPVGPRPPKGFVGLKNAGATCYMNSVIQQLYMIPSIRNSILAIEGTDSDTDDDMFGEEKQDSESNVDPRDDVFGYPHQFEDKPALSKTEDRKDYNIGVLRHLQVIFGHLAASRLQYYVPRGFWKQFRLWGEPVNLREQHDALEFFNSLVDSLDEALKALGHPAMLSQVLGGSFADQKICQGCPHRYECEESFTTLNVDIRNHQNLLDSLEQYIKGDLLEGANAYRCEKCDKKVDTVKRLLIKKLPPVLAIQLKRFDYDWERECAIKFNDYFEFPRELDMEPYTVRGVAKLEGDNVNPDSQLIQQNEQSESETAGSTKYRLVGVLVHSGQASGGHYYSYIIQRNGKDGERNRWYKFDDGDVTECKMDDDEEMKNQCFGGEYMGEVFDHMMKRMSYRRQKRWWNAYILFYERTDVIDQDDEMIRCISELTITRPHQIMSPAIERSVRKQNVQFMHNRMQYSLEYFQFVKKLLTCNGIYLNPAPGQDHLLPEAEEITMISIQLAARFLFTTGFHTKKIVRGPASDWYDALCILLRHSKNVRFWFAHNVLFNVSNRFSEYLLECPSAEVRGAFAKLIVFIAHFSLQDGPCPSSFTSPGPPSQACDNLSLSDHLLRAVLNLLRREVSEHGRHLQQYFNLFVMYANLGMAEKSQLLKLNVPATFMLVSLDEGPGPPIKYQYAELGKLYSVVSQLIRCCNVASRMQSSINGNPPLPNPFGDPNLSQPIMPIQQNVADILFVRTSYVKKIIEDCSNSEETIKLLRFCCWENPHFSSTVLSELLWQVAYSYTYELRPYLDLLLQILLIEDSWQTHRIHNALKGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSSCPVAYQILQGNGDLKRKWTWAVEWLGDELERRSYTGNPQYSYNNWSPPVQSNETSNGYFLERSHSARMTLAKACELCPEEVKKDTTGQQIEMEERKEPDDQDAPDEHESSPPEDAPLYPHSSGSQYQQVNGSFCLVFIFIPLHQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LUA1</accession>
    <name>A0A8C4LUA1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ALKBH8</fullName>
            <ecNumber evidence="5">2.1.1.229</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Alkylated DNA repair protein alkB homolog 8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Alpha-ketoglutarate-dependent dioxygenase ALKBH8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">S-adenosyl-L-methionine-dependent tRNA methyltransferase ALKBH8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">ALKBH8</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>5-(carboxymethyl)uridine(34) in tRNA + S-adenosyl-L-methionine = 5-(2-methoxy-2-oxoethyl)uridine(34) in tRNA + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:43208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:10408"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74851"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.1.1.229"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with TRMT112.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alkB family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.229" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4LUA1"/>
    <dbReference type="Ensembl" id="ENSEAST00005017211.2">
        <property type="protein sequence ID" value="ENSEASP00005015827.2"/>
        <property type="gene ID" value="ENSEASG00005011010.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158563"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106335">
        <property type="term" value="F:tRNA (5-carboxymethyluridine(34)-5-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12431">
        <property type="entry name" value="RRM_ALKBH8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1520:FF:000001">
        <property type="entry name" value="Alkylated DNA repair protein alkB homolog 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000313">
        <property type="entry name" value="Alkylated DNA repair protein alkB homolog 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027450">
        <property type="entry name" value="AlkB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015095">
        <property type="entry name" value="AlkB_hom8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051422">
        <property type="entry name" value="AlkB_tRNA_MeTrf/Diox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034256">
        <property type="entry name" value="ALKBH8_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13069:SF21">
        <property type="entry name" value="ALKYLATED DNA REPAIR PROTEIN ALKB HOMOLOG 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13532">
        <property type="entry name" value="2OG-FeII_Oxy_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09004">
        <property type="entry name" value="ALKBH8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 23" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RRM" evidence="24">
        <location>
            <begin position="45"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="25">
        <location>
            <begin position="220"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022884"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00049802"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00067723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00078946"/>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
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        <source>
            <dbReference type="PROSITE" id="PS51471"/>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005015827.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="687" mass="77565" checksum="044B8AC83451EE1E" modified="2023-09-13" version="2">MDSNHQNNYKLNKTEKKFLRKQIKARHTLLRHEGIETVSYATQVLVVANGGLGNGVSRNQLLPVLEKCGPVDALLMPPNKPYSFVRYKTTEESERAYITLNGKEIVDDLGQKIILYLNFVEKAQWKELGLQALPPGLMVVEEIISSEDEKMLLESVNWTEDTENQNFQKSLKHRRVKHFGYEFHYENNNVDKNKPLPGGLPDICDSILEKWLKEGFIKHKPDQLTVNQYEPGHGIPAHIDTHSAFEDEIVSLSLGSEIVMDFKHPDGVTVPVMLPRRSLLVMTGESRYLWTHGITPRKFDTVQASKSHKSGIITSDVGDLTLSKRGIRTSFTFRKVRQTPCNCSYPLVCDSQMKETPSSFPESDKEASQLEREYVHQVYEEIAGHFSSTRHTPWPRVVEFLKALPSGSLVADIGCGNGKYLGVNKELYMIGCDRSQNLVDICRERQFEAFVCDALAVPIHSGSCDACISIAVIHHFATAARRVAAIQELVRLLRPGGKALIYVWAMEQEYNNKKSKYLRENRVSQGKKEEINSDTSVQELLVEQMPVVGNQDSACSVPSTNDFQKGGCDSRKVTDSKLPVHTNRTSFHSQDLLVPWHLKGNPGKIQLVEPFGPVGSHDPSPVFHRYYHVFCAGELEAACQTLSNVSVLQSYYDQGNWSVNNNSAKLEKLQMCINRMSKLWSVHTICY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LW45</accession>
    <name>A0A8C4LW45_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pleckstrin homology domain-containing family H member 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PLEKHH3</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="nucleotide sequence ID" value="XM_014833519.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LW45"/>
    <dbReference type="Ensembl" id="ENSEAST00005015650.2">
        <property type="protein sequence ID" value="ENSEASP00005014405.2"/>
        <property type="gene ID" value="ENSEASG00005010019.2"/>
    </dbReference>
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    <dbReference type="KEGG" id="eai:106826275"/>
    <dbReference type="CTD" id="79990"/>
    <dbReference type="GeneTree" id="ENSGT00940000159764"/>
    <dbReference type="OrthoDB" id="6108017at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd13297">
        <property type="entry name" value="PH3_MyoX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="pleckstrin homology domain-containing family H member 3"/>
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        <property type="entry name" value="pleckstrin homology domain-containing family H member 3 isoform X1"/>
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        <property type="entry name" value="pleckstrin homology domain-containing family H member 3 isoform X2"/>
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    </dbReference>
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        <property type="entry name" value="pleckstrin homology domain-containing family H member 3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MyTH4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051724">
        <property type="entry name" value="Actin_motor_Myosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000857">
        <property type="entry name" value="MyTH4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038185">
        <property type="entry name" value="MyTH4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46049">
        <property type="entry name" value="AGAP003327-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46049:SF5">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY H MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00784">
        <property type="entry name" value="MyTH4"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF21989">
        <property type="entry name" value="RA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00139">
        <property type="entry name" value="MyTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51016">
        <property type="entry name" value="MYTH4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0488">Methylation</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040443251" description="Pleckstrin homology domain-containing family H member 3" evidence="6">
        <location>
            <begin position="19"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="95"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="MyTH4" evidence="9">
        <location>
            <begin position="237"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="FERM" evidence="8">
        <location>
            <begin position="404"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="28"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="552"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="598"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="750"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="782"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00067094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
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            <dbReference type="PROSITE" id="PS50003"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014405.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="793" mass="85126" checksum="6E28995C6444691A" modified="2023-09-13" version="2">MPLPGGLWWLLCCRRGFTLLHRDYGDGELSGDGDEDEDEETFELRTPSPAGGGRSPLDVTLTQPVRSGPVSDRLQSWEETRSLIPEKGPPEDDPDVVVKGWLYREPRGGGARPWLPPRRAWFVLTRDSLDQFSSSGKGARRLGSLVLTSLCSVTGPERRPKETGLWSVTVSGRKHSVRLCSPRQAEAERWAVALREVIASKAPLETPTQLLLRDIQESCGDPEAVALIYRRNPILRHTSGALYAPLLPLPYGVSAPGPGYAPLREEAVRLFLALQALEGARRPGPLMQGVLQTCRDLPALRDELFLQLAKQTSGPAGPPGPPATQDPAALRYWQLLTCMSCTFRPGGAVRGHLLGHLERTEQALPDSELAEFARFIRKALGRTRGRELVPSLAEISALSRRQELLCTVHCPGAGACPVAIDSHTTAGEVARELVGRLGLTRSRNAFALYEQRGAQERALAGGTLVADVLTRFENLAAEEAGLEDSPDSGWRLCLRLHGPLHPEGLSPDGHELPFLFEQAHALLLRGRPPPPDDTLRALAALRLQSLHRDFSPRAPLPRLDRLLPPSAPPREDSPRPVPRPAPSAALLAGAIWSPGLAKRQAERARRGGAGRTAGSPAREGGGGAGTAAAVLGGWKRLRGMGRAEAMAAYLALAAQCPGFGAARYDVLELSTEPGGGAPQKLCLGLGAKAMSFSRPGETEPIHSVSYGHVAACQLMGPHTLALRMGESQLLLQSPQVEEIMQLVNAYLANPSPERPCSSPSPPGQDLPGTSPRSQHPGLDEPQGQSGCLGQLQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LWC0</accession>
    <name>A0A8C4LWC0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Putative histone-lysine N-methyltransferase PRDM6</fullName>
            <ecNumber evidence="2">2.1.1.361</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">PR domain zinc finger protein 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">PR domain-containing protein 6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">PRDM6</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Liu"/>
                <person name="G."/>
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                <person name="Guo"/>
                <person name="J."/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
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                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
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                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser. Also, involved in the degradation of oxidized and glycated proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of an N-acetyl or N-formyl amino acid from the N-terminus of a polypeptide.</text>
            <dbReference type="EC" id="3.4.19.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S9C family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.1" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4LXF8"/>
    <dbReference type="Ensembl" id="ENSEAST00005018154.2">
        <property type="protein sequence ID" value="ENSEASP00005016702.2"/>
        <property type="gene ID" value="ENSEASG00005011529.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013172"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008242">
        <property type="term" value="F:omega peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050435">
        <property type="term" value="P:amyloid-beta metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000047">
        <property type="entry name" value="Acylamino-acid-releasing enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000043">
        <property type="entry name" value="acylamino-acid-releasing enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045550">
        <property type="entry name" value="AARE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002471">
        <property type="entry name" value="Pept_S9_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF4">
        <property type="entry name" value="ACYLAMINO-ACID-RELEASING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19283">
        <property type="entry name" value="APEH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50993">
        <property type="entry name" value="Peptidase/esterase 'gauge' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00708">
        <property type="entry name" value="PRO_ENDOPEP_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylamino-acid-releasing enzyme N-terminal" evidence="15">
        <location>
            <begin position="48"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="14">
        <location>
            <begin position="523"/>
            <end position="736"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF19283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005016702.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="737" mass="81692" checksum="39B31B6ADD2E0BBE" modified="2023-09-13" version="2">MERQVLLSEPEEAAALYRGLSRQPALSAACLGPEVTTQYGGRYRTVHTEWTQRDLERMENIRFCRQYLVFHDGDSVVFAGPAGNSVETRGELLSRDSPSGAMKAVLRKAGGTGLGEEKQFLEVWEKNRKLKSFNLSALEKHGPVYEDDCFGCLSWSHSETHLLYVAEKKRPKAESFFQTKALDIGGSDDEMARLKKPDQAIKGDQFVFYEDWGENMVSKSTPVLCVLDVESGNISVLEGVPESVSPGQAFWAPGDTGVVFVGWWHEPFRLGIRFCTNRRSALYYVDLIGGKCELLSDDSLAVSSPRLSPDQCRIVYLQYPSLVPHHQCSQLCLYDWYTKVTSVVVDVVPRQLGENFSGIYCNLLPLGCWSADSQRVVFDSAQHSRQDLFAVDTQMHSVTSLTAGGSGGSWKLLTIDRDLMVVQFSTPSLPPSLKVGFLPPAGKEQSVLWVSLEEAEPIPDISWGIRVLQPPPEQENVQYAGLDFEAILLQPSDPPDKTQVPMVVMPHGGPHSSFVTAWMLFPAMLCKMGFAVLLVNYRGSTGFGQDSILSLPGNVGNQDVKDVQFAVEQVLQEEHFDAGRVALMGGSHGGFLSCHLIGQYPETYSACVARNPVINIASMMGSTDIPDCSVSCRCVVEAGFPYSSDCLPDLSVWAEMLDKSPIKYTPQVKTPLLLMLGQEDRRVPFKQGVEYYRALKARKVPVRLLLYPKSTHTLSEVEVESDSFMNAVLWLRTHLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LYJ1</accession>
    <name>A0A8C4LYJ1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein unc-45 homolog B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">UNC45B</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Acts as a co-chaperone for HSP90 and is required for proper folding of the myosin motor domain. Plays a role in sarcomere formation during muscle cell development. Is necessary for normal early lens development.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with HSP90 in an ATP-independent manner. Interacts with UBE4B; the interaction may target UNC45B for proteasomal degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
            <location evidence="1">A band</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Myofibril</location>
            <location evidence="2">Sarcomere</location>
            <location evidence="2">Z line</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LYJ1"/>
    <dbReference type="Ensembl" id="ENSEAST00005017532.2">
        <property type="protein sequence ID" value="ENSEASP00005016132.2"/>
        <property type="gene ID" value="ENSEASG00005011201.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157654"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031672">
        <property type="term" value="C:A band"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051879">
        <property type="term" value="F:Hsp90 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007517">
        <property type="term" value="P:muscle organ development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000043">
        <property type="entry name" value="Unc-45 myosin chaperone B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000153">
        <property type="entry name" value="Unc-45 myosin chaperone B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000025">
        <property type="entry name" value="Unc-45 myosin chaperone B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024660">
        <property type="entry name" value="UCS_central_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45994">
        <property type="entry name" value="FI21225P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45994:SF2">
        <property type="entry name" value="PROTEIN UNC-45 HOMOLOG B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11701">
        <property type="entry name" value="UNC45-central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="7" id="KW-0517">Myogenesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
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            <begin position="6"/>
            <end position="39"/>
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            <begin position="330"/>
            <end position="489"/>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00004556"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00020768"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022473"/>
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            <dbReference type="ARBA" id="ARBA00022541"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022803"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053758"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
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            <dbReference type="ARBA" id="ARBA00062249"/>
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    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF11701"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005016132.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="931" mass="103422" checksum="61C11021E09C11AD" modified="2023-09-13" version="2">MAEGEAAQLKEEGNRHFQLQDYKAATKSYSQALKLTKDKALLATLYRNRAACGLKTENYVQAASDASRAIDINSSDIKALYRRCQALEHLGKLDQAFKDVQRCATLEPRNQNFQETLRRLNTSIQEKLRVQFSTDSRVQKMFEILLDPNSDADKLEKSANNLIVLGREEAGAEKIFQNNGVALLLQLVDTKKPELMLAAVRTLSGMCSGHRARATAILHAVRIDRICSLMAPENEEMSLAVCNLLQAIIDSLSGEDKQEHRGKEEAVVLDTKKDLKQITSHLLDMLVSKKVSGQGRDQALNLLNKNVPRKDLAIHDNSRTIYVVDNGLRKILKVVGQVPDLPSCLPLTDNTRMLASILINKLYDDLRCDPERDHFRKICEEYITGKFDPQDMDKNVIAIQTVSGILQGPFDLGNQLLGLKGVMEMMVALCGSERETDQLVAVEALIHASTKLSRATFIITNGVSLLKEIYKTTKNEKIKIRTLVGLCKLGSAGGTDYALRQFAEGSTEKLAKQCRKWLCNASIDTRTRRWAVEGLAYLTLDADVKDDFVQDIPALQAMFELAKASTSDKTILYSVATTLVNCTNSYDVKEVIPELVQLAKFSKQHVPEEHPKDKKDFVDMRVKRLLKAGVISVLACMVKADNAILTDQTKELLARVFLALCDNPKDRGTIVAQGGGKALIPLALEGTDVGKVKAAHALAKIAAVSNPDIAFPGERVYEVVRPLVSLLDTQRDGLQNYEALLGLTNLSGRSDKLRQKIFKEKALPDIENYMFENHDQLRQAATECMCNMVVNKEVQERFLADGNDRLKLVVLLCGEDDDKVQNAAAGALAMLTAAHKKLCLKMTQVTTQWLEILQRLCLHDRLSVQHRGLVIAYNLLAADAELAKKLVESELLEILTVVGKQEPDEKKAAVVQTARECLVKCMDYGFIKPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LZL5</accession>
    <name>A0A8C4LZL5_EQUAS</name>
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        <recommendedName>
            <fullName evidence="12">C-type lectin domain family 18 member A</fullName>
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    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106823449</name>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014829162.3"/>
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        <property type="nucleotide sequence ID" value="XM_070500576.1"/>
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    <dbReference type="Ensembl" id="ENSEAST00005021169.2">
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        <property type="gene ID" value="ENSEASG00005013412.2"/>
    </dbReference>
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    <dbReference type="KEGG" id="eai:106823449"/>
    <dbReference type="GeneTree" id="ENSGT00900000141128"/>
    <dbReference type="OMA" id="CAEKVQF"/>
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        <property type="component" value="Chromosome 28"/>
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        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="CAP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00037">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.33.10:FF:000014">
        <property type="entry name" value="C-type lectin domain family 18 member A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C-type lectin domain family 18 member A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.33.10">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014044">
        <property type="entry name" value="CAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035940">
        <property type="entry name" value="CAP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001283">
        <property type="entry name" value="CRISP-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10334">
        <property type="entry name" value="CYSTEINE-RICH SECRETORY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00188">
        <property type="entry name" value="CAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00837">
        <property type="entry name" value="V5TPXLIKE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00198">
        <property type="entry name" value="SCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF55797">
        <property type="entry name" value="PR-1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 6" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0430">Lectin</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034397548" description="C-type lectin domain family 18 member A" evidence="7">
        <location>
            <begin position="31"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="8">
        <location>
            <begin position="233"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="9">
        <location>
            <begin position="311"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="256"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
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            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005019513.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="451" mass="49572" checksum="73358F591FCBBAF3" modified="2022-01-19" version="1">MLLSEPTAGMGGCRPGLLPMLLALLGIAWAEMQSLQLREEQAPMPGALSRKESFLLLSLHNRLRSRVHPPAANMQRMDWSESLAQWAQARAALCGAPAASPVSASGATPQLGWNVQLLPVGSASFIHVVGLWFSEGQQYSHAAAECDPNATCTHYTQLVWATSSQLGCGRHPCSGAQAELEAFVCAYSPGGNWEVNGKTIVPYKKGAWCSLCTASVPGCFKAWDHAGGLCEVPRNPCRMSCRNHGHLNVSTCHCHCPPGYTGRYCQVRCSVQCVHGRFREEECSCICDVGYGGAQCATKVHFPFHTCDLRIDGDCFMVSSEADTYYGAKMKCQGKGGVLAQIESQKVQDILAFYLGRLETTNEVTDSDFETRNFWIGLTYKTTKDSFRWTTGEHQSFTSFAFGQPDNQGFGNCVELQASAAFNWNDQRCKTRNRYICQFAQEHISRWDPGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M1F8</accession>
    <name>A0A8C4M1F8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">RALBP1 associated Eps domain containing 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">REPS1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
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                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
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                <person name="D.D."/>
                <person name="Li"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
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                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="Zhang"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="24" key="3">
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                <consortium name="Ensembl"/>
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            <name>Zn(2+)</name>
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        <text evidence="17">Binds 1 zinc ion per subunit.</text>
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    <comment type="subunit">
        <text evidence="15">Interacts with COMP.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
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            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
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        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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        <property type="term" value="P:chondrocyte differentiation"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="Peptidase_M12B"/>
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        <property type="entry name" value="Peptidase_M12B_N"/>
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        <property type="entry name" value="TSP1_rpt"/>
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        <property type="entry name" value="ADAMTSFAMILY"/>
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        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
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        <property type="entry name" value="TSP-1 type 1 repeat"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="13 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="6 17 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 21" id="KW-0732">Signal</keyword>
    <keyword evidence="10 17 19" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0865">Zymogen</keyword>
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        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
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            <begin position="28"/>
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            <begin position="248"/>
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            <begin position="1539"/>
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            <end position="1140"/>
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            <begin position="1190"/>
            <end position="1281"/>
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            <begin position="1034"/>
            <end position="1064"/>
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            <begin position="1108"/>
            <end position="1118"/>
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            <begin position="1165"/>
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        </location>
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        <location>
            <position position="395"/>
        </location>
    </feature>
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        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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        <location>
            <position position="251"/>
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            <label>2</label>
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        <location>
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        <location>
            <position position="398"/>
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            <note>catalytic</note>
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        <location>
            <position position="404"/>
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        <ligand>
            <name>Zn(2+)</name>
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        <location>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>1</label>
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        <location>
            <position position="456"/>
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        <ligand>
            <name>Ca(2+)</name>
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        </ligand>
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        <location>
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        <ligand>
            <name>Ca(2+)</name>
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            <label>2</label>
        </ligand>
    </feature>
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            <begin position="324"/>
            <end position="378"/>
        </location>
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    <feature type="disulfide bond" evidence="18">
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
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    <feature type="disulfide bond" evidence="18">
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    </feature>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="1598" mass="178072" checksum="15B0649628DA7093" modified="2023-09-13" version="2">MPCAQRSWLANLSVVAQLLNFGALCYGRQPLPGPVHFPDRRQEHFIKALPEYHVVAPVRVDASGHFLSYGLHHPISSSRRKRDLGGPEDQVYYRISHEEKDLFFNLTVNQGFLSKSYIVERRYGNLSHVKMVASSGPPCHLRGTVLQQGTRIGTAALSACHGLTGLFHLPHGDFFIEPIKKHPLAEGGYHPHIIYRRQRRRVPEMKEPTCGLKDSLGTSQKLELQREKWERNNLRSRGLSRRSISKERWVETLVVADTKMIEYHGSENVESYILTIMNMVTGLFHNPSIGNAIHIVVVRLILLEEEEQGLKIVHHAEKTLSSFCKWQKSINPKSDLNPAHHDVAVLLTRKDICAGVNRPCETLGLSHLSGMCQPHRSCNINEDSGLPLAFTIAHELGHSFGIQHDGKENDCEPIDRHPFIMSRQFQYNPTPLTWSKCSKEYITRFLDRGWGFCLDDIPKKKGLKSKIVAPGVIYDVHHQCQLQYGPNATFCQEVENVCQTLWCSVKGFCRSKLDAAADGTRCGEKKWCMAGKCITVGKKPESIPGGWGRWSPWSHCSRTCGAGAQSAERLCNSPEPKFGGKYCTGERKRYRLCNVHPCRPDTPTFRQLQCSEFDTVPYKNAFYHWFPVFSSAHPCELYCRPIDGQFSEKMLDAVIDGTPCFEGGNSRNVCINGICKMVGCDYEIDSNATEDRCGVCLGDGSACQTVRKMFKQKEGSGYVDIGLIPKGARDIRIMEVEGAGNFLAIRSEDPEKYYLNGGFIIQWNGNYKLAGTIFQYDRKGDLEKLIATGPTNESVWIQLLFQVTNPGIKYEYTIRKDGLDNDVEKLVYFWQYGHWTECSVTCGTGIRRQTAHCVKQGHGMVKATFCDPETQPNGRQKKCYEKDCPPRWWAGEWEACSATCGPHGEKKRTVLCIQTMGSDEQALQAKDCQHLLKPKTLISCNRDILCPSDWTVGNWSECSVSCGGGVRIRSVTCAKNHDEPCDVTRKPNSRALCGLQQCPSSRRVLKPNKGTIFNGKKLPTPEQDPLKPIPPTTSSPRMLTTPTVPQSMSPSVWASNSPAPTTASKGDLDGKQWQNTSTQTELDSHYVVSTGGTSQPILTSWSLSIQPNKETVSSSDPGPTSEGDLLATTMSGSDLSSSSNPVTWQVTAFYDTLTKEPETEIHSGSGEQPENKNGNNSVIWTKIRVPGNDASVERSTEIPLEPPPTPYLRGSSLWPPLSTVTEGSLPRQRPATPQNATSRAEGLVTEKPADTPFPLGGDHQPTPSEKSVNPNPPELPSDMNLTQSSGQVLTEEDATSLIAEGFLLNASNYKQLSTSRSPAYWVVGNWSECSTTCGLGAYWRHVECSTHLDSDCAAIQRPDPAKRCHLRPCAGWKVGNWSKCSRNCSGGFQIREIQCVDSRDHRNLRPFHCQFLAGIPPPLSMSCNTEPCEEWQVEPWSQCSRSCGGGVQERIVTCPGDFCDWTKKPTSTRPCNAHPCCHWATGHWDLCTASCGGGFQKRTVHCVSSEDNKTEDQDQCLCDHEPRPPEFQKCNRQACRKSDDLLCTKDKLSTSFCQTLKTMKKCSVPTVRVQCCLSCSQTHIARTRRPRKPQSFKSPKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MAB2</accession>
    <name>A0A8C4MAB2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="28">Cyclic nucleotide-gated channel alpha-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="29">Olfactory cyclic nucleotide-gated channel subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="34" type="primary">CNGA2</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="34 35" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
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                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
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                <person name="A."/>
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                <person name="M."/>
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                <person name="D.D."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="26">Pore-forming subunit of the olfactory cyclic nucleotide-gated channel. Operates in the cilia of olfactory sensory neurons where chemical stimulation of the odorant is converted to an electrical signal. Mediates odorant-induced cAMP-dependent Ca(2+) influx triggering neuron depolarization. The rise of intracellular Ca(2+) levels potentiates the olfactory response by activating Ca(2+)-dependent Cl(-) channels, but it also serves as a negative feedback signal to desensitize the channel for rapid adaptation to odorants. Conducts cAMP- and cGMP-gated ion currents, with permeability for monovalent and divalent cations.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>Cs(+)(in) = Cs(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78555"/>
            <dbReference type="ChEBI" id="CHEBI:49547"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>Li(+)(in) = Li(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78551"/>
            <dbReference type="ChEBI" id="CHEBI:49713"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>NH4(+)(in) = NH4(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28747"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>Rb(+)(in) = Rb(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:78547"/>
            <dbReference type="ChEBI" id="CHEBI:49847"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="27">The olfactory cyclic nucleotide-gated channel is an heterotetramer composed of CNGA2, CNGA4 and CNGB1b subunits with 2:1:1 stoichiometry.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22">Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family. CNGA2 subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSEASP00005023662.2"/>
        <property type="gene ID" value="ENSEASG00005016083.2"/>
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        <property type="component" value="Chromosome X"/>
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        <property type="term" value="C:intracellular cyclic nucleotide activated cation channel complex"/>
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        <property type="entry name" value="Helix hairpin bin"/>
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        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR032406">
        <property type="entry name" value="CLZ_dom"/>
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    <dbReference type="InterPro" id="IPR050866">
        <property type="entry name" value="CNG_cation_channel"/>
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        <property type="entry name" value="cNMP-bd_CS"/>
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    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
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        <property type="entry name" value="cNMP-bd_dom_sf"/>
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        <property type="entry name" value="Ion_trans_dom"/>
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    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED CATION CHANNEL SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45638:SF3">
        <property type="entry name" value="CYCLIC NUCLEOTIDE-GATED OLFACTORY CHANNEL"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF16526">
        <property type="entry name" value="CLZ"/>
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        <property type="entry name" value="cNMP"/>
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    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
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    </dbReference>
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    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="8" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="6" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="17" id="KW-0114">cAMP</keyword>
    <keyword evidence="5" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="4" id="KW-0140">cGMP</keyword>
    <keyword evidence="13" id="KW-0142">cGMP-binding</keyword>
    <keyword evidence="14 30" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="19" id="KW-0407">Ion channel</keyword>
    <keyword evidence="15" id="KW-0406">Ion transport</keyword>
    <keyword evidence="18" id="KW-1071">Ligand-gated ion channel</keyword>
    <keyword evidence="16 32" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 32" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 32" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="140"/>
            <end position="160"/>
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    <feature type="transmembrane region" description="Helical" evidence="32">
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            <begin position="172"/>
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    <feature type="transmembrane region" description="Helical" evidence="32">
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            <begin position="278"/>
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    <feature type="transmembrane region" description="Helical" evidence="32">
        <location>
            <begin position="350"/>
            <end position="372"/>
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    <feature type="domain" description="Cyclic nucleotide-binding" evidence="33">
        <location>
            <begin position="454"/>
            <end position="568"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="31">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="30">
        <location>
            <begin position="594"/>
            <end position="646"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="31">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000309"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004272"/>
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            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="30">
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    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
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    </evidence>
    <evidence type="ECO:0000313" key="34">
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    </evidence>
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            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="664" mass="76109" checksum="864BA94EE64EB3F3" modified="2023-09-13" version="2">MTEKSNGVKSSPANNHNHHAPPTIKANGKDDRRTSNRPQSAADDDTSSELQRLAEMDAPQRGRGGFRRIAHLVGVIREWANKNFREEEPRPDSFLERFRGPELQTVTTQQGDGKGNKDGEGKGTKKKFELFVLDPAGDWYYRWLFVIAMPVLYNWCLLVARACFSDLQKGYYLVWLVLDYFSDVIYIADLFIRLRTGFLEQGLLVKDTKKLRDSYIHTLQFKLDVASIIPTDLIYFAVGIHSPELRFNRLLHFARMFEFFDRTETRTSYPNIFRISNLVLYILVIIHWNACIYYAISKSIGFGVDTWVYPNITDPEYGYLAREYIYCLYWSTLTLTTIGETPPPVKDEEYLFVIFDFLIGVLIFATIVGNVGSMISNMNATRAEFQAKIDAVKHYMQFRKVSKEMEAKVIRWFDYLWTNKKSVDEREVLKNLPAKLRAEIAINVHLSTLKKVRIFQDCEAGLLVELVLKLRPQVFSPGDYICRKGDIGKEMYIIKEGKLAVVADDGVTQYALLSAGSCFGEISILNIKGSKMGNRRTANIRSLGYSDLFCLSKDDLMEAVTEYPDAKKVLEERGREILMKEGLLDENEVAASMEVDVQEKLEQLETNMETLYTRFGRLLAEYTGAQQKLKQRITVLEIKMKQNNEDDYLSDGINSPEPAAAEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MAC8</accession>
    <name>A0A8C4MAC8_EQUAS</name>
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        <submittedName>
            <fullName evidence="2">Inhibitor of CDK, cyclin A1 interacting protein 1</fullName>
        </submittedName>
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    <gene>
        <name evidence="2" type="primary">INCA1</name>
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    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="Liu"/>
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                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
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                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
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                <person name="Liu"/>
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                <person name="D.D."/>
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                <person name="Zhou"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <end position="20"/>
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    <sequence length="247" mass="28054" checksum="4D0DFCBCDAC09BAF" modified="2023-09-13" version="2">MRRLTPPTQPSPVIQVQEDGDNLIPFAKCSRVVSRSPPPSLPSQSLRLTPQRYGDIFWENLSQRPSPTWMEEHYIPPLLRATGCSQPGLYPPEGLPPPEMLCRRKRRRPHLVGTQQGPGGIPARVRAVTYHLEDLRRRQRIINELKKAQWGSPEATSEPLVPDEEGCEFPSTTKYPALEAERTTYPWEEDHFLTPGRAQLLWSPWSPLGQEGSCLSRQLSSLPSYSTVTASRNPLYNPWGMELQPEE</sequence>
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    <name>A0A8C4MAH2_EQUAS</name>
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            <fullName evidence="7">Rho GTPase-activating protein 29</fullName>
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        <alternativeName>
            <fullName evidence="8">Rho-type GTPase-activating protein 29</fullName>
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    </protein>
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        <name evidence="17" type="primary">ARHGAP29</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
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                <person name="Y."/>
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                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
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                <person name="J."/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
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                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
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                <person name="Jiang"/>
                <person name="Q."/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="R."/>
                <person name="Yu"/>
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                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
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                <person name="G."/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has strong activity toward RHOA, and weaker activity toward RAC1 and CDC42. May act as a specific effector of RAP2A to regulate Rho. In concert with RASIP1, suppresses RhoA signaling and dampens ROCK and MYH9 activities in endothelial cells and plays an essential role in blood vessel tubulogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with PTPN13/PTPL1. Interacts with RAP2A via its coiled coil domain. Interacts with RASIP1.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSEASP00005023010.2"/>
        <property type="gene ID" value="ENSEASG00005015647.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 16"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0030165">
        <property type="term" value="F:PDZ domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0051056">
        <property type="term" value="P:regulation of small GTPase mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="CDD" id="cd20816">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.555.10:FF:000016">
        <property type="entry name" value="Rho GTPase activating protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000038">
        <property type="entry name" value="Rho GTPase activating protein 29"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Rho GTPase-activating protein 29"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.555.10">
        <property type="entry name" value="Rho GTPase activation protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
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    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054713">
        <property type="entry name" value="GMIP/FCHO2-like_FCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057028">
        <property type="entry name" value="RHG29_45_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051025">
        <property type="entry name" value="RhoGAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000198">
        <property type="entry name" value="RhoGAP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15228:SF7">
        <property type="entry name" value="RHO GTPASE-ACTIVATING PROTEIN 29"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR15228">
        <property type="entry name" value="SPERMATHECAL PHYSIOLOGY VARIANT"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00620">
        <property type="entry name" value="RhoGAP"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00324">
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48350">
        <property type="entry name" value="GTPase activation domain, GAP"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50238">
        <property type="entry name" value="RHOGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 11 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="F-BAR" evidence="16">
        <location>
            <begin position="192"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="14">
        <location>
            <begin position="613"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="domain" description="Rho-GAP" evidence="15">
        <location>
            <begin position="672"/>
            <end position="887"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="471"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="542"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="989"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1120"/>
            <end position="1268"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="361"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="482"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="542"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="992"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1191"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1232"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="1258"/>
            <end position="1268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00040783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00042921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005023010.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1268" mass="142387" checksum="54B58AC613E08A9A" modified="2023-09-13" version="2">MIAHKQKKTKKKRVLGPGQLSTDVTTSEMGLKSISSNSIFDPDYVKELVNDIRKFSHMLLYLKEAILSDCFKEVIHIRLDELLRVLKSVMNKHQNLNSVDLQNAAEMLTAKVKAVNFTEVNEENKNDLFREVFSSIETLAVTFGNILTNFLMGDVGNDSLLRLPVSRESKSFENVSVESVDSPNEKGNFSPIELDTVLLKNTDSVELALSYAKTWSKYTKNIVSWVEKKLNLELESTRNIVKLAEATRTNIGLQEFMPLQSLFTNALLNDIESSHLLQQTIGALQANKFVQPLLGRKNEMEKQRKEIKELWKQEQNKMLETETALKKAKLLCMQRQDEYEKAKSSMFRAEEEHLCSSGGLAKNLNKQLEKKRRLEEEALQKVEEANELYKVCVTNVEERRNDLENTKREILTQLRKLVFQCDLTLKAVTVNLFQMQHLQAASLASNLQSLCDSAKLYDPGQEYSEFVKATNSAEEEKVDGNVNKQLTNTPHSSGYGPADSLEDVVRLPDSSNKIEEDRCSNSADITGPSFIRSWTFGMFSDSESTGGSSESRSLDSESISPGDFHRKLPRTPSSGTMSSADDLDEREPPSPSEAGPNSLGTFKKTLMSKAALTHKFRKLRSPTKCRDCEGIVVFQGVECEECLLVCHRKCLENLVIICGHQKLLGKIHLFGAEFTHLARKEPDGIPFILKICASEIENRAMCLQGIYRVCGNKIKTEKLCQALENGMHLVDISEFSSHDICDVLKLYLRQLPEPFILFRLYKEFIDLAKEIQLVNEEQETKKDSSEDKKWPSTSIEINRILLKSRDLLRQLPASNFNSLHYLIVHLKRVVDHAEENKMNSRNLGLIFGPSLIRPRPTTAPVTISSIAEYSNQARLVEFLITYSQKIFDGSLQPQEVVVCSTGGVAPQVDQGCFPKPLLSPEERDPEHSMKSLFFSSKEDIHTADNESKIFEPAKSFEESERKQNALEKCDACLIDSKVRFLVDQELESASQKMEDERSKPLPLKPSRETNSVERHPPRTKIRPVSLPVDRLLLLASLPNERNGRNAGSVNSERFCKNPAFEGVNRKDTPTVVCYNFDGFDQQTLQKTQEKQYEKNDLTAKTAVIAPGVLQERGVTMSVRLGGDHPVSAAQPSKPYTDPVRSARQVSERRSSDSCPPTSARAPRTLQPQHWTTFYKPHAPAGSGRGDEEKPAAASAAGPPGTAHAPQGPAPKSVPNSESGSACAGQPGSQPKENSEERDLPDVHPTCQRPRLKRMQQFEDLEDEIPQFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MEH8</accession>
    <name>A0A8C4MEH8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Pregnancy zone protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106837423</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005023786.2">
        <property type="protein sequence ID" value="ENSEASP00005021925.2"/>
        <property type="gene ID" value="ENSEASG00005014949.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163775"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02897">
        <property type="entry name" value="A2M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1940:FF:000002">
        <property type="entry name" value="Alpha-2-macroglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000312">
        <property type="entry name" value="Alpha-2-macroglobulin like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000001">
        <property type="entry name" value="CD109 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1930:FF:000001">
        <property type="entry name" value="CD109 isoform 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001815">
        <property type="entry name" value="pregnancy zone protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1930:FF:000002">
        <property type="entry name" value="PZP, alpha-2-macroglobulin like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.690:FF:000001">
        <property type="entry name" value="PZP, alpha-2-macroglobulin like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.130.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1540">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1930">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.690">
        <property type="entry name" value="Alpha-macroglobulin, receptor-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009048">
        <property type="entry name" value="A-macroglobulin_rcpt-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036595">
        <property type="entry name" value="A-macroglobulin_rcpt-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050473">
        <property type="entry name" value="A2M/Complement_sys"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011625">
        <property type="entry name" value="A2M_N_BRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041813">
        <property type="entry name" value="A2M_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047565">
        <property type="entry name" value="Alpha-macroglob_thiol-ester_cl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011626">
        <property type="entry name" value="Alpha-macroglobulin_TED"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001599">
        <property type="entry name" value="Macroglobln_a2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019742">
        <property type="entry name" value="MacrogloblnA2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002890">
        <property type="entry name" value="MG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041555">
        <property type="entry name" value="MG3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040839">
        <property type="entry name" value="MG4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412">
        <property type="entry name" value="MACROGLOBULIN / COMPLEMENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11412:SF116">
        <property type="entry name" value="PREGNANCY ZONE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00207">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07703">
        <property type="entry name" value="A2M_BRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07677">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01835">
        <property type="entry name" value="MG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17791">
        <property type="entry name" value="MG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17789">
        <property type="entry name" value="MG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07678">
        <property type="entry name" value="TED_complement"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01360">
        <property type="entry name" value="A2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01359">
        <property type="entry name" value="A2M_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01361">
        <property type="entry name" value="A2M_recep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01419">
        <property type="entry name" value="Thiol-ester_cl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49410">
        <property type="entry name" value="Alpha-macroglobulin receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00477">
        <property type="entry name" value="ALPHA_2_MACROGLOBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5 10" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0882">Thioester bond</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040500417" description="Pregnancy zone protein" evidence="10">
        <location>
            <begin position="25"/>
            <end position="1493"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin bait region" evidence="11">
        <location>
            <begin position="457"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-2-macroglobulin" evidence="12">
        <location>
            <begin position="744"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-macroglobulin receptor-binding" evidence="13">
        <location>
            <begin position="1382"/>
            <end position="1469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM01361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005021925.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1493" mass="166770" checksum="94A72E58588660F5" modified="2023-09-13" version="2">MKGNQLPNPVLILLFLLLPRDASATSKPQYLVLVPSQLYSGVPEKACILLNHLNETVTLSVTLEYGRQSRNLLTDLVTEKNSFYCSPFIIPELPSSSAFITVEVKGPTLQFINRKSVQITQAESLVFVQTDKPTYKPGQTVRFRIVSVDVSFHPLNEMFPVVYIENPKRNRIFQWQSLKLQGGLSQLSLPLSAEPALGPYKVVLQKESGRKIEHSFEVIEYVLPKFEVQVKMPKTIGFLDKEFVVSVCGLYTYGKPVSGLVTINICRKYSRYYSTCHGKHSQSICEEFSQQADNEGCFTQFVKTKIFQLRQKLYDMTIQVEAKVREEGTGLELTGHGSCEITNTLSKLKFIKVDSHYRCGLPFFGQILLVDEKDQPIPNETIVVAVDLNRYRSSYTTNEHGLVDISIDTTNFTSSFITVTVTYKENNQCFDHWWLSEFHTQAQHTAKRIFSPSKSYVHLEPVAGTMTCGQTQEIRAHYILNGQMLQDEKDLTFYYLIQARGSISQSGIHVLSIKQGDMKGVFSFSFQVKSDNAPSAQLLVYTILPNGEIVADTGKLEIENCFANKVNLSFSSARNLPASNTTLKVTATPHSLCALRAVDQSVLLMKPEAELSPQSVYNLLPEKNLFSVSYRGPANEDGQKCIHAEDITHNGIIYTPKQILDDDDVYSIFESVGLNIFTNSKIHKPRFCQLPHGYPAMPMPYLGAVPQGLALASGAAGAVYPTMMREEFPAMEVKGTVRKYFPETWIWDLVPLDSSGGSGLAKQVPDTITEWKANALCLSKTTGLGLSPIISLQVFQPFFLELTLPYSVVRGEDFILKATVFNYLFHCIRVSVQLDISPAFSAVPVEKNEDSHCVCGNGQKTVSWAVTPKSPGKVNFTATAEALRSQELCGNEVPEVPAFGQKDTVVKPLLVELEGIEKEETFNTLLCASETGVPEKLSLKVPSDVVKGSARATYSVLGDILGSAMQNLQNLLQMPYGCGEQNMVLFVPNIYVLNYLSETQQLTEKIKSKAISHLISGYQRQLNYKHSDGSYSTFGDHDGRSQGNTWLTAFVLKSFAQARSYIYVEDSHITNSLTWLSQKQKENGCFQSSGLLLNNAIKGGVDDEVTLSAYITIALLEMPLPVTHQVVRNALFCLENAWRSTSEAQGSLVYTKALLAYAFALAGNQAKRSELLESLDKEAVKEEDSIHWQRPGKFQEAKELYYQPRAPSAEVEMTSYSLLAYLTAQPAPSSEDLSVASRIVKWITKQQNPNGGFSSTQDTVVALQALSKYGAATFTKREKEAIVTVKSSRTFSEEFQVDDTNRLLLQEVELPEIPGEYSTTVSGSGCVYLQTSLRYNILPKKEGKVPFTLKVDPLAKNCDGIDVHRKFQIHINISYTGERASSNMVIVDVKMVSGFIPVKTSVKKLQERPQIQRTEVSTNHVLIYFEELTNQTLSFTFSVEQRIQIKNLKPATVKAYDYYETEEFTIEEYSDPCLAGMLEHGNLWTSSDELLWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MFA1</accession>
    <name>A0A8C4MFA1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Family with sequence similarity 83 member G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">FAM83G</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM83 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044600458.1">
        <property type="nucleotide sequence ID" value="XM_044744523.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MFA1"/>
    <dbReference type="Ensembl" id="ENSEAST00005028004.2">
        <property type="protein sequence ID" value="ENSEASP00005025793.2"/>
        <property type="gene ID" value="ENSEASG00005017609.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837916"/>
    <dbReference type="KEGG" id="eai:106837916"/>
    <dbReference type="CTD" id="644815"/>
    <dbReference type="GeneTree" id="ENSGT00940000157932"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd09187">
        <property type="entry name" value="PLDc_FAM83G_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.870.10:FF:000004">
        <property type="entry name" value="protein FAM83H isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.870.10">
        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050944">
        <property type="entry name" value="FAM83"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012461">
        <property type="entry name" value="SACK1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16181">
        <property type="entry name" value="PROTEIN FAM83A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16181:SF29">
        <property type="entry name" value="PROTEIN FAM83A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07894">
        <property type="entry name" value="SACK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56024">
        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Scaffolding anchor of CK1" evidence="5">
        <location>
            <begin position="15"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="75"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="360"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="450"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="518"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="79"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="90"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="360"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="452"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="568"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="581"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="799"/>
            <end position="813"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025793.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="813" mass="89127" checksum="3B398A9E88451263" modified="2023-09-13" version="2">MAFSQVQCLDDSHVNWRSSESKPEFFYSEEQRLALEALVARGPDAFYEVLKRENIRDFLSELELKRILETIEVYDPGSEDPRDTSRPQEPEDNGLGDGEEVSGAGGIPTEAEPLPSLEYWPQKSDRSIPQLDLGWPDTVAYRGVTRASVYMQPPIDGQAHIKEVVRKMISQAQKVIAVVMDMFTDVDIFKDLLDAGFKRKVAVYIIVDESNVKYFLHMCERACMHLGHLKNLRVRSSGGTEFFTRSATKFKGALAQKFMFVDGDRAICGSYSFTWSAARTDRNVISVLSGQVVEMFDRQFQELYLMSHSVSLKGIPMEKEPEPEPIVLPSVVPLVPSGTVAKKLVNPKYALVKAKSADEIAKSSSEKQDVKRPPGPQGAALAERPGDLPELLLPIHPGLLNLERANMFEYLPTWVEPDPEPGSDILGYINIIDPNIWNPQPSQMNRIKIRDTSQASAQWRQSQDCSPAPAPRAPQDGVPAENGLPRGDPESRPPVPKPRTVPVADLLAQDSSGVGWALETPAEGVPQNGADHGLSRTAGLGHAPLQRQLSTPQDDPDGRGPAIPNGLDGEEEENDDDYVTLSDQDSLSGSSGLGSGPRRLSVASSSVSDEYFEVRERLAPLRRHHSEQVANGPAQSPRRQLSAPHMTHGTFGGPQAVSSWAQDQEREEVDTPRRTQAPRSVDKKAQGPKFHYYGVPASGTRDKDGFPRLSRTTGPARCRPTAEGVQSSTRKAGPTVAGPHHWQAKGGPGPRTLPDPGSPLADGRATEEHPSPFGIPYSKLSQSKHLKARTGGGQWASSDSKRRAQAPRDRKDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MIB6</accession>
    <name>A0A8C4MIB6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1</fullName>
            <ecNumber evidence="3">1.14.11.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Lysyl hydroxylase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">PLOD1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils. Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-lysyl-[collagen] + 2-oxoglutarate + O2 = (5R)-5-hydroxy-L-lysyl-[collagen] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:16569"/>
            <dbReference type="Rhea" id="RHEA-COMP:12751"/>
            <dbReference type="Rhea" id="RHEA-COMP:12752"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:133442"/>
            <dbReference type="EC" id="1.14.11.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homodimer. Identified in a complex with P3H3 and P3H4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Rough endoplasmic reticulum membrane</location>
            <topology evidence="13">Peripheral membrane protein</topology>
            <orientation evidence="13">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="1.14.11.4" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4MIB6"/>
    <dbReference type="Ensembl" id="ENSEAST00005028198.2">
        <property type="protein sequence ID" value="ENSEASP00005025975.2"/>
        <property type="gene ID" value="ENSEASG00005016643.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234558"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902494">
        <property type="term" value="C:catalytic complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Zhao"/>
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                <person name="S."/>
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                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT SAP25"/>
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            <end position="319"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="36895" checksum="CBA0A5B3184C9CF8" modified="2023-09-13" version="2">MLRARSSRRLAGRAGGSPATPPARGRPRERAHVALPETRWAPASRRGLERRDMLPWPPPWGGAGEEQAPQEEGPSAGSDPGEAWDSGDEALGEPGTPPPDSPQPRSPRPSWTRREQLLSQAPPGTAAEQWPRTPVPLSPQMAWEVAPSRMTLLAPWDLNYDSKAGPWLVWGPSSASGVAFSGRTLCHPSFWPLYEAASGRNPRPLAPATGHQNGEHAGFPVMCCEDVFLSDPLLPRGQRVPLYLSEASQQVLGSLKLLLPPPIMSPWVLPTSSPGCSTAWLSGPELIALTGLLQMSQGEPRPSSSISGAPTPPAGPPDPLSDHPGLSGGGSCSHCADPSLPGTPDSHCL</sequence>
</entry>
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    <accession>A0A8C4MMQ5</accession>
    <name>A0A8C4MMQ5_EQUAS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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                <person name="J."/>
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                <person name="G."/>
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                <person name="J."/>
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                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MS03"/>
    <dbReference type="Ensembl" id="ENSEAST00005032354.2">
        <property type="protein sequence ID" value="ENSEASP00005029768.2"/>
        <property type="gene ID" value="ENSEASG00005020234.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182890"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000029">
        <property type="entry name" value="GLI family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000111">
        <property type="entry name" value="GLI family zinc finger 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001158">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000051">
        <property type="entry name" value="zinc finger protein 585A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000070">
        <property type="entry name" value="zinc finger protein 689 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050752">
        <property type="entry name" value="C2H2-ZF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384">
        <property type="entry name" value="FINGER PUTATIVE TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384:SF242">
        <property type="entry name" value="ZINC FINGER PROTEIN 628"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="14">
        <location>
            <begin position="15"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="209"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="237"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="265"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="316"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="344"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="372"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="456"/>
            <end position="483"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005029768.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="483" mass="53961" checksum="68F8B5AAA5733175" modified="2023-09-13" version="2">TTVSSIKAIILPTLVTFKDLVVDFTREEWGLLGPTQRILFHDMMLRNYSNLVSLAEASLSPLHPCHLCVQRERSLGGRVLWCRFLTEHQNRPPGVQAPTDCVHKKGLPCVSVLGQGRPCQGEGVCTEACDSFPDLRHPQGVHTEEGTLACGGWEDAFGKNTSFLSSHPPDPPQNPHGEKPYECHVCCKAFSLSLSLAEHMRCHTGEKPYVCQECGKAFSQSSSLIKHLRTHTGEKPYICSECGRAFSQRSSLTKHQRVHTGERPSVCRECGKAFSQSSYLTQHLKIHSGKKPFMCGECRKPFSDSLALVHTGEQPYTCGKCGKAFSQSKFLTQHERIHTEEKPFVCGDCGRAFMQSSSLALYLRTHTGEKPYKCNECGKAYIQMSHLTEHYRVHTREQPYMCNVCGKAFGRGTHLLQHQRVHVGAKPFTCGVCRKTFPEAAALILHQRIHAGEKPFECHKCSKSFSQSRSLAQHQSIHSQGRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MTF3</accession>
    <name>A0A8C4MTF3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">V-set and transmembrane domain-containing protein 2B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">VSTM2B</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044614948.1">
        <property type="nucleotide sequence ID" value="XM_044759013.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MTF3"/>
    <dbReference type="Ensembl" id="ENSEAST00005033429.2">
        <property type="protein sequence ID" value="ENSEASP00005030747.1"/>
        <property type="gene ID" value="ENSEASG00005020939.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106834973"/>
    <dbReference type="CTD" id="342865"/>
    <dbReference type="GeneTree" id="ENSGT00940000161356"/>
    <dbReference type="OMA" id="YQCRVSD"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000804">
        <property type="entry name" value="V-set and transmembrane domain containing 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051102">
        <property type="entry name" value="IgSF_V-set/TM_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207:SF27">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034474391" description="V-set and transmembrane domain-containing protein 2B" evidence="10">
        <location>
            <begin position="29"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="11">
        <location>
            <begin position="14"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="160"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="288" mass="30273" checksum="345622158929B1DC" modified="2022-01-19" version="1">MEQRNRLGALGYLPPLLLHALLLFVADATFTEVPKDVTVREGDDIEMPCAFRASGATSYSLEIQWWYLKEPPRELLHELALSVPGARSKVTNKDATKISTVRVQGNDISHRLRLSAVRRQDEGVYECRVSDYSDDDTQEHKAQALLRVLSRFAPPNMQAAEAVSHIQSSGPRRHGPASAANANHGGATGAAGRATSEPGRGDKSPPPASPPAARGPGVPEAAAASAAHAAATTVAAAAAASSASLPPSRAVLLRQRHGSGTGPSYAPDPLLSLLLLALHQLLGLLVGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MXB6</accession>
    <name>A0A8C4MXB6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Protein transport protein Sec61 subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SEC61B</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Necessary for protein translocation in the endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the SEC61-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014715207.1">
        <property type="nucleotide sequence ID" value="XM_014859721.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MXB6"/>
    <dbReference type="SMR" id="A0A8C4MXB6"/>
    <dbReference type="Ensembl" id="ENSEAST00005036398.2">
        <property type="protein sequence ID" value="ENSEASP00005033385.2"/>
        <property type="gene ID" value="ENSEASG00005022860.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106842923"/>
    <dbReference type="KEGG" id="eai:106842923"/>
    <dbReference type="CTD" id="10952"/>
    <dbReference type="GeneTree" id="ENSGT00390000003561"/>
    <dbReference type="OrthoDB" id="5401193at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044322">
        <property type="term" value="C:endoplasmic reticulum quality control compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005784">
        <property type="term" value="C:Sec61 translocon complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048408">
        <property type="term" value="F:epidermal growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030671">
        <property type="entry name" value="Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13509">
        <property type="entry name" value="SEC61 SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006398">
        <property type="entry name" value="Sec61_beta_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR006398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005033385.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="96" mass="9974" checksum="5FAFF4197A62FA49" modified="2023-09-13" version="2">MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGRTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MXN6</accession>
    <name>A0A8C4MXN6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Regulator of telomere elongation helicase 1</fullName>
            <ecNumber evidence="17">5.6.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17 20" type="primary">RTEL1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15 17">A probable ATP-dependent DNA helicase implicated in telomere-length regulation, DNA repair and the maintenance of genomic stability. Acts as an anti-recombinase to counteract toxic recombination and limit crossover during meiosis. Regulates meiotic recombination and crossover homeostasis by physically dissociating strand invasion events and thereby promotes noncrossover repair by meiotic synthesis dependent strand annealing (SDSA) as well as disassembly of D loop recombination intermediates. Also disassembles T loops and prevents telomere fragility by counteracting telomeric G4-DNA structures, which together ensure the dynamics and stability of the telomere.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="16 17">Interacts with TERF1. Interacts (via PIP-box) with PCNA; the interaction is direct and essential for suppressing telomere fragility. Interacts with MMS19; the interaction mediates the association of RTEL1 with the cytosolic iron-sulfur protein assembly (CIA) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="17">Nucleus</location>
        </subcellularLocation>
        <text evidence="17">Colocalizes with PCNA within the replication foci in S-phase cells.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the helicase family. RAD3/XPD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.-" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4MXN6"/>
    <dbReference type="Ensembl" id="ENSEAST00005036925.2">
        <property type="protein sequence ID" value="ENSEASP00005033874.2"/>
        <property type="gene ID" value="ENSEASG00005023163.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182970"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
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        <property type="term" value="C:chromosome, telomeric region"/>
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<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N0M6</accession>
    <name>A0A8C4N0M6_EQUAS</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
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                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014686673.3">
        <property type="nucleotide sequence ID" value="XM_014831187.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4N0M6"/>
    <dbReference type="Ensembl" id="ENSEAST00005037219.2">
        <property type="protein sequence ID" value="ENSEASP00005034135.2"/>
        <property type="gene ID" value="ENSEASG00005023341.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106824726"/>
    <dbReference type="KEGG" id="eai:106824726"/>
    <dbReference type="CTD" id="114793"/>
    <dbReference type="GeneTree" id="ENSGT00940000155515"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008360">
        <property type="term" value="P:regulation of cell shape"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2220:FF:000001">
        <property type="entry name" value="Formin-like 1, isoform CRA_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000036">
        <property type="entry name" value="Formin-like protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000045">
        <property type="entry name" value="Formin-like protein 3 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014767">
        <property type="entry name" value="DAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010472">
        <property type="entry name" value="FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043592">
        <property type="entry name" value="FMNL_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014768">
        <property type="entry name" value="GBD/FH3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010473">
        <property type="entry name" value="GTPase-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857">
        <property type="entry name" value="FORMIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45857:SF5">
        <property type="entry name" value="FORMIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06367">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06371">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01139">
        <property type="entry name" value="Drf_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01140">
        <property type="entry name" value="Drf_GBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51231">
        <property type="entry name" value="DAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51232">
        <property type="entry name" value="GBD_FH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GBD/FH3" evidence="5">
        <location>
            <begin position="23"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="domain" description="FH2" evidence="6">
        <location>
            <begin position="613"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="domain" description="DAD" evidence="4">
        <location>
            <begin position="1036"/>
            <end position="1075"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="514"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="381"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="978"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="521"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="544"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="579"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005034135.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1089" mass="123830" checksum="36D3DA9636725B55" modified="2023-09-13" version="2">MGNAGSMDSQQTDFRGHSVPLKLPMPEPGELEERFAIVLNAMNLPPDKARLLRQYDNEKKWELICDQERFQVKNPPHTYIQKLKGYLDPAVTRKKFRRRVQESTQVLRELEISLRTNHIGWVREFLNEENKGLDVLVEYLSFAQYAVTFDFESVESTVESSVDKSKPWSRSIEDLHRGSNLPSPVGNSVSRSGRHSALRYNTLPSRRTLKNSRLVSKKDDVHVCIMCLRAIMNYQYGFNMVMSHPHAVNEIALSLNNKNPRTKALVLELLAAVCLVRGGHEIILSAFDNFKEVCGEKQRFEKLMEHFRNEDNNIDFMVASMQFINIVVHSVEDMNFRVHLQYEFTKLGLDEYLDKLKHTESDKLQVQIQAYLDNVFDVGALLEDAETKNAALERVEELEENISHLSEKLQDTENEAMSKIVELEKQLMQRNKELDVVREIYKDANTQVHTLRKMVKEKEEAIQRQSTLEKKIHELEKQGTIKIQKKGDGDIAILPVVASGTLSMASDMAVGQITGAASTGPLPPPPPALPPSSDTPEAVQNGPATPPMPPPPPPPPPPPPPPPPPPPPPLPGPAADTVPAPPLPPPPPPSAPPLPGTSSPTVVFNSGLAAVKIKKPIKTKFRMPVFNWVALKPNQINGTVFNEIDDERILEDLNVDEFEEIFKTKAQGPAIDLSSSKQKITQKGSNKVTLLEANRAKNLAITLRKAGKTADEICKAIHVFDLKTLPVDFVECLMRFLPTENEVKVLRLYERERKPLENLSDEDRFMMQFSKIERLMQKMTIMAFIGNFAESLQMLTPQLHSIIAASVSIKSSQKLKKILEIILALGNYMNSSKRGAVYGFKLQSLDLLLDTKSTDRKQTLLHYISNVVKEKYHQVSLFYNELHYVEKAAAVSLENVLLDVKELQRGMDLTKREYTMHDHNTLLKEFIFNNEGKLKKLQDDAKIAQDAFDDVVKYFGENPKTTPPSVFFPVFVRFVKAYKQAEEENELRKKQEQALMEKLLEQEALMEQQDPKSPSHKSKRQQQELIAELRRRQVKDNRHVYEGKDGAIEDIITDLRNQPYRRADAVRRSVRRRFDDQNLRSVNGAEITM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N3U9</accession>
    <name>A0A8C4N3U9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">E3 ubiquitin-protein ligase SH3RF1</fullName>
            <ecNumber evidence="7">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Plenty of SH3s</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">RING-type E3 ubiquitin transferase SH3RF1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">SH3 domain-containing RING finger protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">SH3RF1</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Lamellipodium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Golgi apparatus</location>
            <location evidence="4">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SH3RF family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4N3U9"/>
    <dbReference type="Ensembl" id="ENSEAST00005034315.2">
        <property type="protein sequence ID" value="ENSEASP00005031570.2"/>
        <property type="gene ID" value="ENSEASG00005021491.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155875"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001237">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd16748">
        <property type="entry name" value="RING-HC_SH3RF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11785">
        <property type="entry name" value="SH3_SH3RF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000077">
        <property type="entry name" value="E3 ubiquitin-protein ligase SH3RF1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000091">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase SH3RF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000118">
        <property type="entry name" value="Putative E3 ubiquitin-protein ligase SH3RF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000001">
        <property type="entry name" value="Sorbin and SH3 domain-containing protein 1 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050384">
        <property type="entry name" value="Endophilin_SH3RF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035816">
        <property type="entry name" value="SH3RF1/SH3RF3_SH3_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167:SF44">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SH3RF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14167">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="2"/>
    </dbReference>
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                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation.</text>
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        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
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    <comment type="similarity">
        <text evidence="4">Belongs to the cytochrome c oxidase subunit 6B.</text>
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        <property type="component" value="Chromosome 26"/>
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        <property type="entry name" value="Cyt_c_Oxidase_VIb"/>
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        <property type="entry name" value="Cytochrome c oxidase subunit 6B1"/>
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        <property type="entry name" value="Cytochrome c oxidase, subunit VIb"/>
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        <property type="entry name" value="COX6B-like"/>
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    <dbReference type="InterPro" id="IPR036549">
        <property type="entry name" value="CX6/COA6-like_sf"/>
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        <property type="entry name" value="Cyt_c_oxidase_su6B"/>
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        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 6B"/>
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        <property type="entry name" value="COX6B"/>
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    <keyword evidence="3 5" id="KW-1015">Disulfide bond</keyword>
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        <location>
            <begin position="40"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000278-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="86" mass="10142" checksum="07B878685AF0BB9C" modified="2022-01-19" version="1">MAEDIKSKIKNYQTAPFDSRFPNQNQTRNCWQNYLDFHRCEKAMTAKGGDVSVCEWYRRVYKSLCPLSWVSAWDDRRAEGTFPGKI</sequence>
</entry>
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    <accession>A0A8C4PFL8</accession>
    <name>A0A8C4PFL8_EQUAS</name>
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        <recommendedName>
            <fullName evidence="11">Olfactory receptor</fullName>
        </recommendedName>
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        <name type="scientific">Equus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PFL8"/>
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    <dbReference type="GeneTree" id="ENSGT01150000286940"/>
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        <property type="component" value="Chromosome 20"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF121">
        <property type="entry name" value="OLFACTORY RECEPTOR 52A4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="62"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="101"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="200"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="240"/>
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        </location>
    </feature>
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        <location>
            <begin position="43"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001374.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="342" mass="38639" checksum="05C0DA43728792FE" modified="2023-09-13" version="2">MSITNGTVFMPSVLTFIGIPGLETVQCWIGIPFCAMYVIALIGNSLLLIIIKSEPSLHEPMYIFLAMLGATDIALSTSIVPKMLGIFWLHLPEIYFDACLFQMWLIHTFQGIESGVLLAMALDRYVAICYPLRHATIFTRQLVIHIGVGVALRPAVLVIPCLSLIKCRLKLYRTRLISHTYCEHMALVKLATEDVYINKFYGLLGAFIVGGLDFILTTLCYIQIFITVFHLPQKEARLKAFNTCVPHIFVFFQFYLLAFFSFFTHRSGSYIPIWILYPSIHTYHLVQPLPSGSTFPQPFCLWGEDQAHLRQGSKSILFQRSGLTLDRDSTPYGFNGSSVGDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PG39</accession>
    <name>A0A8C4PG39_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Tubulin-folding cofactor B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Cytoskeleton-associated protein 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Cytoskeleton-associated protein CKAPI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Tubulin-specific chaperone B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">TBCB</name>
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    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer. Involved in regulation of tubulin heterodimer dissociation. May function as a negative regulator of axonal growth.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state. Cofactors B and E can form a heterodimer which binds to alpha-tubulin and enhances their ability to dissociate tubulin heterodimers. Interacts with GAN. Interacts with DCTN1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the TBCB family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014693796.1">
        <property type="nucleotide sequence ID" value="XM_014838310.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PG39"/>
    <dbReference type="SMR" id="A0A8C4PG39"/>
    <dbReference type="Ensembl" id="ENSEAST00005003194.2">
        <property type="protein sequence ID" value="ENSEASP00005002919.2"/>
        <property type="gene ID" value="ENSEASG00005002241.2"/>
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    <dbReference type="GeneID" id="106829271"/>
    <dbReference type="KEGG" id="eai:106829271"/>
    <dbReference type="CTD" id="1155"/>
    <dbReference type="GeneTree" id="ENSGT00940000156119"/>
    <dbReference type="OrthoDB" id="5295208at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
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    <dbReference type="GO" id="GO:0035371">
        <property type="term" value="C:microtubule plus-end"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="term" value="F:alpha-tubulin binding"/>
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        <property type="term" value="P:cell differentiation"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
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        <property type="term" value="P:tubulin complex assembly"/>
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        <property type="entry name" value="Tubulin-folding cofactor B"/>
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        <property type="entry name" value="CAP Gly-rich-like domain"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
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        <property type="entry name" value="TBCB_Ubl"/>
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    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
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        <property type="entry name" value="Ubiquitin-like_domsf"/>
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        <property type="entry name" value="DYNACTIN 1-RELATED MICROTUBULE-BINDING"/>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
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        <location>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="19">
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00058415"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
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            <dbReference type="Ensembl" id="ENSEASP00005002919.2"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8C4PJ00_EQUAS</name>
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        <submittedName>
            <fullName evidence="3">Small integral membrane protein 11</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Q."/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
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                <person name="A."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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    </dbReference>
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    <dbReference type="InterPro" id="IPR042125">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR35975:SF1">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35975">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 11A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="30"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008566.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="58" mass="6808" checksum="AEE5FB58067624B0" modified="2023-09-13" version="2">MNWKVLEHVPLLLYILAAKTLILCLAFAGVKVYQRKRLEAKQRQLEAEKQQQAEKKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PM45</accession>
    <name>A0A8C4PM45_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cationic amino acid transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106839284</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PM45"/>
    <dbReference type="Ensembl" id="ENSEAST00005017319.2">
        <property type="protein sequence ID" value="ENSEASP00005015931.2"/>
        <property type="gene ID" value="ENSEASG00005011074.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154637"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000010">
        <property type="entry name" value="probable cationic amino acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF4">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="229"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="267"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="397"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="461"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="495"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="526"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="552"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="9">
        <location>
            <begin position="524"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="598"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005015931.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="618" mass="66412" checksum="875A24D4D807D78D" modified="2023-09-13" version="2">MGWGLPNTASLARFCQKLNRLKPLEESTTETSLQRCLSTLDLILLGVGATVGSGLYVLSGTVAKEMAGPAVLVSFSIAAVASLLAALCYVEFGARVPRTGSAYLFTYVSLGELWAFLVGWNVLLEYLFGGAAVARASSSYLDAIFSHRIRNFTMAHVGFWQVPFLAQFPDFLAAGVILLASTFVSCGARVSSWLNHILLAMNLLVILFIIILGFVLARPHNWSADEGGFAPFGFSGIMAGAATCFYAFLGFGVITTSSEEAQNPKRAVPMAIIITLGLVAGAYILVSTVLTLIVPWHRLEPDSALADAFYQRGYSWAAFIVAAGAICAMNTVLLSDIFTLPRIAYAMATDGLFFQVFAHVHPRTQVPVVGILVFGVLMAFLALLLDLQTLVQFRSICTLLTFTFVATSTIVLHFHKSLPASSLGPASPVGTEQASAPEPGQLRPALRPYLGFLGGCRPGAAVAWALGVLVASAITLDCVLVFGDSALHLPPWGHTLLLLLSSVVFLLSLLVLGAHQQQRRQDTFQVPMVPLTPALSILLNVLLMLQLSYLTWLSLSVWLLIGLMVYFGYGIWHSKENQQELPGLTATHGSLEETAQALQPLSQDPAQEPGHTEQPDSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PMD8</accession>
    <name>A0A8C4PMD8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">DNA repair endonuclease XPF</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">DNA excision repair protein ERCC-4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">ERCC4</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Catalytic component of a structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair, and which is essential for nucleotide excision repair (NER) and interstrand cross-link (ICL) repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="18">Heterodimer composed of ERCC1 and ERCC4/XPF. Interacts with SLX4/BTBD12; this interaction is direct and links the ERCC1-ERCC4/XPF complex to SLX4, which may coordinate the action of the structure-specific endonuclease during DNA repair.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the XPF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PMD8"/>
    <dbReference type="Ensembl" id="ENSEAST00005017905.2">
        <property type="protein sequence ID" value="ENSEASP00005016480.2"/>
        <property type="gene ID" value="ENSEASG00005011416.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004394"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070522">
        <property type="term" value="C:ERCC4-ERCC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000110">
        <property type="term" value="C:nucleotide-excision repair factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990599">
        <property type="term" value="F:3' overhang single-stranded DNA endodeoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001094">
        <property type="term" value="F:TFIID-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034644">
        <property type="term" value="P:cellular response to UV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905765">
        <property type="term" value="P:negative regulation of protection from non-homologous end joining at telomere"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904357">
        <property type="term" value="P:negative regulation of telomere maintenance via telomere lengthening"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901255">
        <property type="term" value="P:nucleotide-excision repair involved in interstrand cross-link repair"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061819">
        <property type="term" value="P:telomeric DNA-containing double minutes formation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009650">
        <property type="term" value="P:UV protection"/>
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    <dbReference type="CDD" id="cd20078">
        <property type="entry name" value="XPF_nuclease_XPF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000002">
        <property type="entry name" value="DNA repair endonuclease XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000040">
        <property type="entry name" value="DNA repair endonuclease XPF isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006167">
        <property type="entry name" value="XPF"/>
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    <dbReference type="InterPro" id="IPR047520">
        <property type="entry name" value="XPF_nuclease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00596">
        <property type="entry name" value="rad1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150:SF0">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0227">DNA damage</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ERCC4" evidence="22">
        <location>
            <begin position="697"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="470"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="673"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="482"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00060353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00064560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00072370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00078823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005016480.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="930" mass="105929" checksum="891096B7F5353169" modified="2023-09-13" version="2">MSGSWCWNCWTRTGWSCVPAGSARTGSSTTSSGCTATRRAWCWCSTRSRPRRVNQDRADRLQDRLEGGPLVFSHSTLAANLALEYFINQLKIEGVEHLPRRVTNEITSNSRYEVYTQGGVIFATSRILVVDFLTDRIPSDLITGILVYRAHRIIESCQEAFILRLFRQKNKRGFIKAFTDNAVAFDTGFCHVERVMRNLFVKKLYLWPRFHVAVNSFLEQHKPEVVEIHVSMTPAMLAIQTAILDILNACLKELKCHNPSLEVEDLSLENAIGKPFDKTIRHYLDPLWHQLGAKTKSLVQDLKILRTLLQYLSQYDCITFLNLLESLRATEKAFGQNSGWLFLDSSTSMFINARARVYNVPDDKMSKKGKMSEKMEIKEEQETKKELVLESNPKWEALTEVLKEIEAENKESEALGGPGQVLICASDDRTCSQLREYITVGAEAFLLRLYRKTFEKDSKAEEVWMKFRQEDSSKRMAKSNKRPKDPHNKEKPSTKERTLKKKKRRLTLTQMIGKPEELEEEEDVKEGCPRDISSSPESCFEEIKHEEFDLNLSSDSAYGILKDPLTIIHPLLGCSDPYALTRVLHEVEPRYVVLYDAELTFVRQLEIYRASRPGKPLRVYFLIYGGSTEEQRYLTALRKEKEAFEKLIREKASMVVPEEREGRDETNLDLVRGSVSTGVPTDTRKAGGQEQNGTQQSIIVDMREFRSELPSLLHRRGIDIEPVTLEVGDYILTPEMCVERKSISDLIGSLNSGRLYSQCIAMSRYYKRPVLLIEFDPSKPFSLTSRGALYHEISSNDISSKLTLLTLHFPRLRILWCPSPHVSAELFEELKQNKPQPDAATAMAVTADSETLPESEKYNPGPQDFLLKMPGVNAKNCRSLMNHVKNIAELASLSQDQLAGVLGNAANAKQLYDFIHTSYAEVVSKGKMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PN10</accession>
    <name>A0A8C4PN10_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Single-pass membrane and coiled-coil domain-containing protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SMCO4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMCO4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PN10"/>
    <dbReference type="Ensembl" id="ENSEAST00005019377.2">
        <property type="protein sequence ID" value="ENSEASP00005017846.2"/>
        <property type="gene ID" value="ENSEASG00005012324.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015987"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027960">
        <property type="entry name" value="DUF4519"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34644">
        <property type="entry name" value="SINGLE-PASS MEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34644:SF2">
        <property type="entry name" value="SINGLE-PASS MEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15012">
        <property type="entry name" value="DUF4519"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="30"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="9"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005017846.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="62" mass="7084" checksum="132ED60162425357" modified="2023-09-13" version="2">MRQLKGKPKKETSKDKKERKQAMQEARQQITTVVLPTLAVVVLLIVVFVYVATRPTVTDSTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PP92</accession>
    <name>A0A8C4PP92_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PP92"/>
    <dbReference type="Ensembl" id="ENSEAST00005022230.2">
        <property type="protein sequence ID" value="ENSEASP00005020477.1"/>
        <property type="gene ID" value="ENSEASG00005014092.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00860000135739"/>
    <dbReference type="OMA" id="FGCWGYC"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="78" mass="8674" checksum="CEEF2DF88CD6285E" modified="2022-01-19" version="1">MIRGTTQRPTEKTAGPRRPFFIGSRPEPVFCGGIFLSLFGCWGYCARCGDSAHWSRDLTLRCWLCPCLSRSAHILAGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PPC4</accession>
    <name>A0A8C4PPC4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protein Wnt</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">WNT11</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Ligand for members of the frizzled family of seven transmembrane receptors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
            <location evidence="1 11">Extracellular space</location>
            <location evidence="1 11">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the Wnt family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PPC4"/>
    <dbReference type="Ensembl" id="ENSEAST00005022456.2">
        <property type="protein sequence ID" value="ENSEASP00005020683.2"/>
        <property type="gene ID" value="ENSEASG00005014224.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158413"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19343">
        <property type="entry name" value="Wnt_Wnt11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2460.20:FF:000001">
        <property type="entry name" value="Wnt homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005817">
        <property type="entry name" value="Wnt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043158">
        <property type="entry name" value="Wnt_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018161">
        <property type="entry name" value="Wnt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027:SF7">
        <property type="entry name" value="PROTEIN WNT-11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12027">
        <property type="entry name" value="WNT RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00110">
        <property type="entry name" value="wnt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01349">
        <property type="entry name" value="WNTPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00097">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00246">
        <property type="entry name" value="WNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 11" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020683.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="540" mass="57963" checksum="1D61343FBDA94CF7" modified="2023-09-13" version="2">MSYRTTRTGRALALPLIPSPDGDTRAREEEGTCPESHRGEAAADPPSNPGLAPPGGPGAPGRERALTRGAQAEGDPAARAAVLDARTPDRNNLPGPRGPDQALPSPAACRGRGGGGGSPGPPGAARTEPAVAGGLGPSALWDPGGPSSLWASWAGHGSPAAACAQDARRDSGRRLLQRATRRRRATMRARPQLCEALLFALALQTGVCYGIKWLALSKTPAALALNQTQHCKQLEGLVSAQVQLCRSNLELMRTVVHAAREATKACRRAFSDMRWNCSSIELAPNYLLDLERGTRESAFVYALSAAAISHAIARACTTGDLPGCSCGPVPGEPPGPGNRWGGCADNLSYGLLMGAKFSDAPMKVKKTGSQANKLMRLHNSEVGRQALRASLEMKCKCHGVSGSCSIRTCWKGLQELRDVAADLKTRYLSATKVVHRPMGTRKHLVPKDLDIRPVKDSELVYLQSSPDFCMKNEKVGSHGTQDRQCNKTSHGSDSCDLMCCGRGYNPYTDRVVERCHCKYHWCCYVTCRRCERTVERYVCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PQ19</accession>
    <name>A0A8C4PQ19_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Solute carrier family 35 member B4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SLC35B4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleotide-sugar transporter family. SLC35B subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014694878.1">
        <property type="nucleotide sequence ID" value="XM_014839392.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PQ19"/>
    <dbReference type="Ensembl" id="ENSEAST00005023382.2">
        <property type="protein sequence ID" value="ENSEASP00005021544.2"/>
        <property type="gene ID" value="ENSEASG00005014742.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106830011"/>
    <dbReference type="KEGG" id="eai:106830011"/>
    <dbReference type="CTD" id="84912"/>
    <dbReference type="GeneTree" id="ENSGT00390000002915"/>
    <dbReference type="OrthoDB" id="999962at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005462">
        <property type="term" value="F:UDP-N-acetylglucosamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005464">
        <property type="term" value="F:UDP-xylose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A3"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR48026">
        <property type="entry name" value="HOMOLOGOUS TO DROSOPHILA SQD (SQUID) PROTEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00076">
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        <location>
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        <location>
            <begin position="126"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="334"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="214"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="346"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00053431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00067973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005032948.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="378" mass="39595" checksum="6437FBBA0AB070A8" modified="2023-09-13" version="2">MEVKPPPGRPQPDSGRRRRRRGEEGHDPKEPEQLRKLFIGGLSFETTDDSLREHFEKWGTLTDCVVMRDPQTKRSRGFGFVTYSCVEEVDAAMCARPHKVDGRVVEPKRAVSREDSVKPGAHLTVKKIFVGGIKEDTEEYNLRDYFEKYGKIETIEVMEDRQSGKKRGFAFVTFDDHDTVDKIVVQKYHTINGHNCEVKKALSKQEMQSAGSQRGRGGGSGNFMGRGGNFGGGGGNFGRGGNFGGRGGYGGGGGSRGSYGGGDGGYNGFGGDGNYGGGPGYSSRGGYGGGGGGGPGYGNQGGGYGGGGGYDGYNEGGNFGGGNYGGGNYNDFGNYSGQQQSNYGPMKGGSFGGRSSGSPYGGGYGSGGGSGGYGSRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I489</accession>
    <name>A0A9L0I489_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Mitotic spindle organizing protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">MZT1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MOZART1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0I489"/>
    <dbReference type="Ensembl" id="ENSEAST00005074029.1">
        <property type="protein sequence ID" value="ENSEASP00005034703.1"/>
        <property type="gene ID" value="ENSEASG00005030213.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005954"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000931">
        <property type="term" value="C:gamma-tubulin ring complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031021">
        <property type="term" value="C:interphase microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033566">
        <property type="term" value="P:gamma-tubulin complex localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051415">
        <property type="term" value="P:microtubule nucleation by interphase microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022214">
        <property type="entry name" value="MZT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28520">
        <property type="entry name" value="MITOTIC-SPINDLE ORGANIZING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28520:SF2">
        <property type="entry name" value="MITOTIC-SPINDLE ORGANIZING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12554">
        <property type="entry name" value="MOZART1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005034703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="87" mass="8927" checksum="6D88E951DF871951" modified="2023-09-13" version="1">MASSSGGGAAAGAAAANLNAVRETMDVLLEISRILNTGLDMETLSICVRLCEQGINPEALSSVIKELRKATEALKGKETGLAPWPSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I6T9</accession>
    <name>A0A9L0I6T9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">small monomeric GTPase</fullName>
            <ecNumber evidence="3">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RIT2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Ras family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="3"/>
    <dbReference type="RefSeq" id="XP_014690186.1">
        <property type="nucleotide sequence ID" value="XM_014834700.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0I6T9"/>
    <dbReference type="SMR" id="A0A9L0I6T9"/>
    <dbReference type="Ensembl" id="ENSEAST00005055151.1">
        <property type="protein sequence ID" value="ENSEASP00005035451.1"/>
        <property type="gene ID" value="ENSEASG00005031366.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106827058"/>
    <dbReference type="KEGG" id="eai:106827058"/>
    <dbReference type="CTD" id="6014"/>
    <dbReference type="GeneTree" id="ENSGT00940000161402"/>
    <dbReference type="OMA" id="FRRKQRH"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044297">
        <property type="term" value="C:cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097447">
        <property type="term" value="C:dendritic tree"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032507">
        <property type="term" value="P:maintenance of protein location in cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010977">
        <property type="term" value="P:negative regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010976">
        <property type="term" value="P:positive regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="595" mass="67040" checksum="03851543C3A67D8E" modified="2023-09-13" version="1">MPILLFLIDTSASMNQRTDLGTSYLDIAKGAVELFLKLRARDPASRGDRYMLVTYDEPPYCIKAGWKENHATFMSELKNLQASGLTTLGQALRSSFDLLNLNRLISGIDNYGQGRNPFFLEPSILITITDGNKLTSTAGIQEELHLPLNSPLPGSELTKEPFRWDQRLFALVLRLPGLASTEPEQLGSVPTDESAITQMCEVTGGRSYCVRTQRMLNQCLESLVQKVQSGVVINFEKTGPDPLPIGEDGFMDSSRPSSSFAAQPWHSCHKLIYVRPNSKTGVPVGHWPIPESFWPDQNLPSLPPRTSHPIVRFSCVDCEPMVIDKLPFDKYELEPSPLTQYILERKSPHTCWQVFVTSSGKYNELGYPFGYLKASTTLTCVNLFVMPYNYPVLLPLLDDLFKVHKLKPNLKWRQAFDNYLKTLPPYYLLPLKKALRMMGAPNLISDNLDCGLSYSVISYLKKLSQQTKLESERILASVGKKPPQEIGIKVKNHSGGGVSLTHSKNFRKLLKEIIGETVPRLTELNTKEFAGFQVGLLNKDLKPQTYRNAYDIPRRGLLDQLTRMRSNLLKTHKFIVGQDEVGKLQNLSLSDFRNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IBB4</accession>
    <name>A0A9L0IBB4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Intraflagellar transport 20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">IFT20</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IBB4"/>
    <dbReference type="Ensembl" id="ENSEAST00005064930.1">
        <property type="protein sequence ID" value="ENSEASP00005038154.1"/>
        <property type="gene ID" value="ENSEASG00005009981.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003413"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030990">
        <property type="term" value="C:intraciliary transport particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097730">
        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061512">
        <property type="term" value="P:protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028172">
        <property type="entry name" value="FT20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31978">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31978:SF1">
        <property type="entry name" value="INTRAFLAGELLAR TRANSPORT PROTEIN 20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14931">
        <property type="entry name" value="IFT20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2 4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="104"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="157" mass="18106" checksum="44EA534BB62B243C" modified="2023-09-13" version="1">MTHLLLAATIPSEQNFSREARRETAMAKDILGEAGLHFDELNKLRVLDPEVTQQTIELKEECKDFVDKIGQFQKIVGGLIELVDQLAKEAENEKMKAIGARNLLKSIAKQREAQQQQLQALIAEKKMQLERYRVEYEALCKVEAEQNEFIDQFIFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0II25</accession>
    <name>A0A9L0II25_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106829208</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0II25"/>
    <dbReference type="Ensembl" id="ENSEAST00005074195.1">
        <property type="protein sequence ID" value="ENSEASP00005036708.1"/>
        <property type="gene ID" value="ENSEASG00005036122.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005075346.1">
        <property type="protein sequence ID" value="ENSEASP00005050295.1"/>
        <property type="gene ID" value="ENSEASG00005036122.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005082105.1">
        <property type="protein sequence ID" value="ENSEASP00005047358.1"/>
        <property type="gene ID" value="ENSEASG00005036122.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183169"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045596">
        <property type="term" value="P:negative regulation of cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000508">
        <property type="entry name" value="Myeloid zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000478">
        <property type="entry name" value="Zinc finger protein 133"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000058">
        <property type="entry name" value="Zinc finger protein 2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000352">
        <property type="entry name" value="zinc finger protein 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000912">
        <property type="entry name" value="Zinc finger protein 660"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000176">
        <property type="entry name" value="zinc finger protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24390">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24390:SF260">
        <property type="entry name" value="ZINC FINGER PROTEIN 383-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="129"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
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    <accession>A0A9L0IKE2</accession>
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        <recommendedName>
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        <name type="scientific">Equus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
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                <person name="Zhao"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Guo"/>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
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        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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            <topology evidence="12">Multi-pass membrane protein</topology>
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    <comment type="similarity">
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
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</entry>
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    <accession>A0A9L0INY3</accession>
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        <submittedName>
            <fullName evidence="3">Transmembrane protein 213</fullName>
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        <name evidence="3" type="primary">TMEM213</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
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        <source>
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    <sequence length="500" mass="52223" checksum="977CC440298CC0CB" modified="2023-09-13" version="1">ASLAPPGPSSEPGNAAHSHHGHGRHHHGHHCCPDHDVGQHHQPPHCGARPHPGQLCAAGGGDVTVRWGHAGGAVGRAAPVQALLGGPPAPQQSGGGSREDHHHLPGQWHSPSPRLGAARFRLISVSLTPTRRCAQARAERANPDFRGEDPESQDAETERFLSTGSTGRRVSFNEAALFEQSRKTHDKGRRYTLTEGDFHHLKNARLTHLHLPPLKIVTIHECDSGEASAAATLHPTATPKASLAIFQPPGKALTGRSVGPSSALPGDPYNSAVGPADFEISPSVSSDSGEGPSLDSGARGAKPAGPGAAAGPGEASPVLQFFTRLRRHASLDGASPYFKVKKWKLEPSQRASSLDTRGSPKRHHFQRQRAASESTEQEDADAPPVDFIQYIASAGDAVTFPPPRPFLASPTSPPPTLGRYFSVDRSVRAGPVGSRPTPFPLGGPGSAHPALQTGGFVPPAPPSPPEAAWLGPPLQHGPEAAAGGDWVRGGGGGGRRGRRE</sequence>
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                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
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        <text evidence="13">Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).</text>
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    <comment type="subunit">
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
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    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 4 subfamily.</text>
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    <dbReference type="GO" id="GO:0070233">
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        <property type="project" value="Ensembl"/>
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        <location>
            <begin position="266"/>
            <end position="289"/>
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    <sequence length="484" mass="54570" checksum="F5AE6AA90C7C8B44" modified="2023-09-13" version="1">MIVHLNDKQENLKRHDPITHPWSEKIMTILGTALGMVFYLLQVVSGESGYAQNGDFEDAELDDYSFSCYSQLEVDGPQHLLSCAFEDPDVNSTNLEFEICEGLLEVKCLNFSKLQETYFIKTKKFLLIGDSTICVKLGGKHITCQKMNIVKRVKPEAPFDVKVIYREEANEFVVTFNTSHLQKKYVKDLLHEVVYRLEKNENDWMHVNISSTKLTLLQRKLQPNAMYEIKVRSIPNTNYFEGFWSEWSPSSHFRTPENNSGKMDPVLLIISIVSFFSVALMVILACVLWKKRIKPIVWPSLPDHKKTLEQLCKKPKKNLNVSFNPESFLDCQIHKVDGIQARDEAEAFLQDTFPPQLDDSEKQRLGGGVQGLNWPSQHAVITPKTFGGESPLRCLSGSVTVCDVPVIPSSRPPDCREGGKNGPHVYQGLLLGAGTTNSTLPPLFPFQSGILTLNPAVQGQPLFTSLGSSQEEAYVTMSSFYQNQ</sequence>
</entry>
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    <accession>A0A9L0ITR5</accession>
    <name>A0A9L0ITR5_EQUAS</name>
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        <submittedName>
            <fullName evidence="12">Growth differentiation factor 1</fullName>
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        <name evidence="12" type="primary">GDF1</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
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                <person name="Fang"/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="Gao"/>
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                <person name="Y."/>
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                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Pan"/>
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                <person name="R."/>
                <person name="Yu"/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
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                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Secreted</location>
        </subcellularLocation>
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        <text evidence="2 8">Belongs to the TGF-beta family.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="Bone morphogenetic protein 4"/>
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        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cystine-knot_cytokine"/>
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        <property type="entry name" value="TGF-b_C"/>
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        <property type="entry name" value="TGF-beta-rel"/>
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    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
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        <property type="entry name" value="EMBRYONIC GROWTH_DIFFERENTIATION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TGF_beta"/>
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        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0339">Growth factor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040172280" evidence="10">
        <location>
            <begin position="30"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="11">
        <location>
            <begin position="246"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="189"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023030"/>
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    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005040479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="370" mass="39164" checksum="DB40FA633A3C1A02" modified="2023-09-13" version="1">MPPPRRGPGRRVLFLLLALLLPSPPPARAPAPPGPAAALLQALGLRDAPRDAPTPRPVPPVMWHLFRRRDPQESRTNLRRTPPGATLRPCHLEELGVAGNIVRHVLDRGAPARPPEPASAAGHCPEWTVVFDLSAVEPAERPSRARLELRFAAAEATAGGWELSVGGRPRALAPGRCCSARRCARPGNAGARRAAGRRLGPQRLGSAQPPPVAGAPPPGPAPAACARLAEASLLLATLDPRLFPPLARPAARGRARGWRGPGGACRARRLYVSFREVGWHRWVIAPRGFLANYCQGQCALPAALSGPGGPPALNHAVLRALMHAAAPGAAGLPCCVPARLSPISVLFFDNSDNVVLRHYEDMVVDECGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IU64</accession>
    <name>A0A9L0IU64_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bardet-Biedl syndrome 5 protein homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">BBS5</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for BBSome complex ciliary localization but not for the proper complex assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Part of BBSome complex, that contains BBS1, BBS2, BBS4, BBS5, BBS7, BBS8/TTC8, BBS9 and BBIP10.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell projection</location>
            <location evidence="1 10">Cilium membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
            <location evidence="2">Centriolar satellite</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the BBS5 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IU64"/>
    <dbReference type="Ensembl" id="ENSEAST00005049858.1">
        <property type="protein sequence ID" value="ENSEASP00005043648.1"/>
        <property type="gene ID" value="ENSEASG00005022847.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002753"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034464">
        <property type="term" value="C:BBSome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060170">
        <property type="term" value="C:ciliary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006606">
        <property type="entry name" value="BBL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030804">
        <property type="entry name" value="BBS5/fem-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014003">
        <property type="entry name" value="BBS5_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21351:SF0">
        <property type="entry name" value="BARDET-BIEDL SYNDROME 5 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21351">
        <property type="entry name" value="BARDET-BIEDL SYNDROME PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07289">
        <property type="entry name" value="BBL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF010072">
        <property type="entry name" value="DUF1448"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00683">
        <property type="entry name" value="DM16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6 10" id="KW-0969">Cilium</keyword>
    <keyword evidence="10" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="5 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="BBSome complex member BBS5 PH" evidence="11">
        <location>
            <begin position="80"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="BBSome complex member BBS5 PH" evidence="11">
        <location>
            <begin position="210"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR010072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005043648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="372" mass="41945" checksum="72B1788DC017B9E6" modified="2023-09-13" version="1">MGPGKRAPYLAEAWISARAVDVVTEEARCLGAGETPLGLCLWAARGPRALVIMSVLDALWEDRDIRFDVSSQQMKTRPGEVLIDCLDSIEDTKGNNGDRGRLLVTNLRIIWHSLAISRINLSVGYNCILNITTRTANSKLRGQTEALYILTKCNNTRFEFIFTNLVPGSPRLFTSVIAVHRAYETSKMYRDFKLRSALIQNKQLRLLPQEHVYDKISGVWNLSSDQGNLGTFFITNVRIVWHANMNDSFNVSIPYLQISGGYVLGFKIDPLEKLQESVKEINSLHKVYSANPIFGVDYEMEEKPQSLEALTVEQVQDDVEIDSDDHTDAFVAYFADGNKQQDREPVFSEELGLAIEKLKDGFTLQGLWEVMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IUH9</accession>
    <name>A0A9L0IUH9_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Empty spiracles homeobox 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">EMX1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EMX homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044628592.1">
        <property type="nucleotide sequence ID" value="XM_044772657.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IUH9"/>
    <dbReference type="Ensembl" id="ENSEAST00005073304.1">
        <property type="protein sequence ID" value="ENSEASP00005044534.1"/>
        <property type="gene ID" value="ENSEASG00005033310.1"/>
    </dbReference>
    <dbReference type="GeneID" id="123286645"/>
    <dbReference type="CTD" id="2016"/>
    <dbReference type="GeneTree" id="ENSGT00940000161476"/>
    <dbReference type="OMA" id="SPHPFFG"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000299">
        <property type="entry name" value="Empty spiracles homeobox 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050877">
        <property type="entry name" value="EMX-VAX-Noto_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24339:SF28">
        <property type="entry name" value="E5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24339">
        <property type="entry name" value="HOMEOBOX PROTEIN EMX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="10">
        <location>
            <begin position="192"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="7">
        <location>
            <begin position="194"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="251"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="252"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005044534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="292" mass="31397" checksum="E2763D0617E1D1FD" modified="2023-09-13" version="1">MCLAGCTPRTAAAPGRGALPRARLPRSALAAATMFQPTAKRGFTIESLVAKDGGTGGGTGGGGAGSHPLAAAASEEPLRPTALNYPHPGAAEAAFVSGFPAAAAAAAGAGRSLYGGPELVFPEAMNHPALTVHPAHQLGASPLQPPHSFFGAQHRDPLHFYPWVLRNRFFGHRFQASDVPQDGLLLHGPFARKPKRIRTAFSPSQLLRLERAFEKNHYVVGAERKQLAGSLSLSETQVKVWFQNRRTKYKRQKLEEEGPESEQKKKGSHHINRWRIATKQANGEDIDVTSND</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
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    <name>A0A9L0J0Z7_EQUAS</name>
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        <recommendedName>
            <fullName evidence="6">TSPY</fullName>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="J."/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the nucleosome assembly protein (NAP) family.</text>
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        <property type="entry name" value="Nucleosome assembly protein"/>
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    <dbReference type="InterPro" id="IPR037231">
        <property type="entry name" value="NAP-like_sf"/>
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        <property type="entry name" value="NAP_family"/>
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    <dbReference type="PANTHER" id="PTHR11875">
        <property type="entry name" value="TESTIS-SPECIFIC Y-ENCODED PROTEIN"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00956">
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            <end position="121"/>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="321" mass="35600" checksum="EC86C4DA392A6C45" modified="2023-09-13" version="1">MASEAGCGEGGARQESRLAFVLGGVLVEAGFREPPGARVGVWAVQAAAGGMAEEVNVLGVEAVREAEALGATEQAGLGREVRLLVEDMEVVEVVAEEDRGQDADEVQQGQEEPGRGPTTARPPLEALEALQLELSSVNARARRAYSRLKHKNSERRKLHLDRRSAIIQSIPGFWAKAILNHPQMSAMISEQDEDMLSYMMDLKVEEVRHPSDCCKIMLFFRKNPYFWNKVIIKEYLIDITGYRESHCTPIQWGQNYERKAYTRRHDQLHFSSNSVGPSLPQDLPWSVCPPRSSARTCGPIPCSTTPGRQAAGDGTERRTCR</sequence>
</entry>
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    <accession>A0A9L0J1Y9</accession>
    <name>A0A9L0J1Y9_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Zinc finger protein 232</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ZNF232</name>
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    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eutheria</taxon>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Fan"/>
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                <person name="Yu"/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        </subcellularLocation>
    </comment>
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    <dbReference type="Ensembl" id="ENSEAST00005066295.1">
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000638">
        <property type="entry name" value="Zinc finger protein 184"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000001">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF54">
        <property type="entry name" value="ZINC FINGER PROTEIN 232"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="10">
        <location>
            <begin position="79"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="307"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="366"/>
            <end position="393"/>
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        <location>
            <begin position="196"/>
            <end position="230"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005046398.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="439" mass="49338" checksum="5DF09A7C8CC30462" modified="2023-09-13" version="1">RGPRAPLQDSSRYGPSPELVQARMAVSPTAAEILALQGRQDQEKIMTMTMEPKEEEQAWGYEPRLHGHHSPSQEIFCGRFRQLCHQETPGPREAGVLCCEWLRPDRHTKEQILELLVLEQFLTILPGELQSCVRGHHPESREEAVTVLEGLEKGLEEAGLQVPGPAHGLAQEEPWEEKAPLGAAQEAPSIRLQPQETQPFPESEQGCLHFPPVVAEDDPEPKDRGSLAQPPITGVESQVFSEKPTTGTFAFEASSEVEGALEQQQRNPEVEGLRRCPAQGKSFGWLVPSYSSHLIIHQRIHSGEKPFRCSDCGKTFNQSSNLVQRQRSHTGEKPYECSACGKSFRWRARVVQHRRIHSGEKGGKPFVCKECGKASRWSSELIRRQRVHARKEPSQCSEGASELSRGWVLHWRNKRKNCRNQEKPLPVCLVPPVRFTGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J4T9</accession>
    <name>A0A9L0J4T9_EQUAS</name>
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        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J4T9"/>
    <dbReference type="Ensembl" id="ENSEAST00005070252.1">
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        <property type="gene ID" value="ENSEASG00005038659.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282511"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15912">
        <property type="entry name" value="7tmA_OR6C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000013">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047132">
        <property type="entry name" value="Olfact_rcpt_6C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454:SF72">
        <property type="entry name" value="OLFACTORY RECEPTOR 6C74"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
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        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
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    <keyword evidence="6" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="107"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="164"/>
            <end position="188"/>
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            <begin position="208"/>
            <end position="227"/>
        </location>
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        <location>
            <begin position="239"/>
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    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="7"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005047393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
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    <accession>A0A9L0J5I8</accession>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
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                <person name="Guo"/>
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                <person name="Min"/>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Guo"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001307">
        <property type="entry name" value="Thiosulphate_STrfase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE RDL2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44086:SF10">
        <property type="entry name" value="THIOSULFATE SULFURTRANSFERASE_RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
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    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
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            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040392782" description="Sulfurtransferase" evidence="2">
        <location>
            <begin position="20"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="54"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005048546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="159" mass="17827" checksum="C98DCB47071F31DD" modified="2023-09-13" version="1">MVPLRLLLWSARWAILGSADSALWGVKSIKGSCHNFCTAVSEDVTYEELKNRLNSKSIMLIDVREKWEILEYGKIPGSVNIPLAEVSEALQMNPKAFHEKYNEAKPSRSDRLVFSCVAGVRSKKALDTAISLGFSSAQHYAGGWKEWATYECSEKKEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J5S1</accession>
    <name>A0A9L0J5S1_EQUAS</name>
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        <alternativeName>
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        <alternativeName>
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        <alternativeName>
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        <alternativeName>
            <fullName evidence="9">Short chain dehydrogenase/reductase family 15C member 1</fullName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
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                <person name="Wang"/>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
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                <person name="Wang"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
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                <person name="J."/>
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                <person name="R."/>
                <person name="Hou"/>
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                <person name="Pan"/>
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                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <reaction evidence="14">
            <text>(R)-3-hydroxybutanoate + NAD(+) = acetoacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20521"/>
            <dbReference type="ChEBI" id="CHEBI:10983"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.30"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Siderophore biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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        <property type="entry name" value="SDR_DHRS6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43477:SF4">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43477">
        <property type="entry name" value="DIHYDROANTICAPSIN 7-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
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    <accession>A0A9L0J6A4</accession>
    <name>A0A9L0J6A4_EQUAS</name>
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        <recommendedName>
            <fullName evidence="4">Mucin-1</fullName>
        </recommendedName>
    </protein>
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        <name evidence="15" type="primary">MUC1</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
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                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
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            <topology evidence="2">Single-pass type I membrane protein</topology>
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        <subcellularLocation>
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        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J6A4"/>
    <dbReference type="Ensembl" id="ENSEAST00005075635.1">
        <property type="protein sequence ID" value="ENSEASP00005047903.1"/>
        <property type="gene ID" value="ENSEASG00005038069.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00710000106874"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
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    <dbReference type="GO" id="GO:0016324">
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005737">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10006:SF19">
        <property type="entry name" value="MUCIN-1"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10006">
        <property type="entry name" value="MUCIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00200">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50024">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="736"/>
            <end position="761"/>
        </location>
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    <feature type="domain" description="SEA" evidence="14">
        <location>
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            <end position="725"/>
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    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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            <end position="185"/>
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    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="198"/>
            <end position="374"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
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            <end position="609"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="791"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="116"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="138"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="162"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="205"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="252"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="300"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="324"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="360"/>
            <end position="374"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="521"/>
            <end position="569"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="570"/>
            <end position="580"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="585"/>
            <end position="604"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="816"/>
            <end position="830"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50024"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005047903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="830" mass="83448" checksum="36835A46CA92E6EF" modified="2023-09-13" version="1">MGSQVPDCPPPQPHPSPTQPSGARKWLVKGGGQLENKQVVRGLSDWSPCTLPEGPGVVGEEEEEFTTTMTLGIQAPFFLLLLLFLVLTAAPTASQVNPTLATSSASTWTMTSADHGTLSLTTSPGSSSGATPAHHDTSSLTTSHAPGSTTTPVYHVSSAQITSPGSSSAATPAHHGTSSLTTSPASGLITTSVHHVTSAPATNGTSSLTTSPASSLAATPAHHGTSSLATSPAPGSTTTPVYHVTSAPATNGTSSLTTSPASSSAATPAHHGTSSLATSPAPGSTTTPVHHVTSAPPTNGTSSLTTSPASSSAATPAHHGTSSLTTSTAPGSTTILVRNVTSAPATSPGSSLATTPAHHGTSSLTTSPAPGSTTTAVHNVTSALAPNGTSSLITSPALGSTTTAVHNVTSALAPNGTSSLITSPALGSTTTAVHNVTSALAPNGTSSLITSPAPGSTTTAVHNVTSALAPNGTSSLITSPAPGSTTTAVHNVTSALATNGTSSLITSPAPGSATTPAHHGTSSLTTSPAPGSTTTPVHHVTSAPATSPASSSAATPTHKSTSTKATTTTVNKGTPSSVPSHHSDTPTTSASHSTRTAASSTNHSIVSPTSSNPFPVRASFFFLSFYISNLQFNSSLEDPSTNYYQELQRNISQLLLEIYKQEDFLSLSAMKFSPGSVVVESTLAFQEGTTDAQEVKTLFDKGATEAARYNLDISRVSVNDVPFPSSTQSGSGVPGWGIALLVLVCVLVALAIIYLVALAVCQCRRKNYGQLDIFPTRDAYHPMSEYPTYHTHGRYVPPGSTRRSPYEEVSSGNGGSSLSYTNPAATSANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J6H3</accession>
    <name>A0A9L0J6H3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Cadherin 19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CDH19</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Cadherins are calcium-dependent cell adhesion proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cell membrane</location>
            <topology evidence="1 13">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014683460.1">
        <property type="nucleotide sequence ID" value="XM_014827974.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J6H3"/>
    <dbReference type="Ensembl" id="ENSEAST00005047696.1">
        <property type="protein sequence ID" value="ENSEASP00005048769.1"/>
        <property type="gene ID" value="ENSEASG00005000282.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822693"/>
    <dbReference type="KEGG" id="eai:106822693"/>
    <dbReference type="CTD" id="28513"/>
    <dbReference type="GeneTree" id="ENSGT00940000160137"/>
    <dbReference type="OrthoDB" id="6252479at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016342">
        <property type="term" value="C:catenin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034332">
        <property type="term" value="P:adherens junction organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016339">
        <property type="term" value="P:calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000902">
        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044331">
        <property type="term" value="P:cell-cell adhesion mediated by cadherin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007043">
        <property type="term" value="P:cell-cell junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.900.10:FF:000001">
        <property type="entry name" value="Cadherin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000008">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000012">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000017">
        <property type="entry name" value="Cadherin 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000014">
        <property type="entry name" value="Cadherin 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000202">
        <property type="entry name" value="cadherin-8 isoform X4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.900.10">
        <property type="entry name" value="TCF3-CBD (Catenin binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039808">
        <property type="entry name" value="Cadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000233">
        <property type="entry name" value="Cadherin_Y-type_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027397">
        <property type="entry name" value="Catenin-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027:SF323">
        <property type="entry name" value="CADHERIN-19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24027">
        <property type="entry name" value="CADHERIN-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01049">
        <property type="entry name" value="CADH_Y-type_LIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12 13" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 13" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 16" id="KW-0732">Signal</keyword>
    <keyword evidence="3 13 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040327418" evidence="16">
        <location>
            <begin position="30"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="605"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="17">
        <location>
            <begin position="76"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="17">
        <location>
            <begin position="157"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="17">
        <location>
            <begin position="265"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="17">
        <location>
            <begin position="379"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="17">
        <location>
            <begin position="478"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005048769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="780" mass="87837" checksum="D237AF1ED6BC8FCA" modified="2023-09-13" version="1">MGWNQKSRMNFSLLLPSLLGISLLWPCLPATENSKTEKVKHSAGPHLRVKRGWMWNQFFVPEEMNRTSYRIGQLRSDLDNGNNSFQYKLLGAEAGSIFVIDERTGDIYARQKLDREERSLYTLRAQVIDTTTGRAVEPESEFVIRVSDINDNEPKFLDEPYEAIVPEMSPEGTLVIQVTASDADDPSSGNNARLLYSLLRGQPYFSIEPTTGVIRISSKMDRELQDEYWVIIQAKDMLGLPGALSGTTSVLIKLSDVNDNKPIFKESLYRLTVSESAPTGTSIGKIMAYDNDIGENAEMDYSIEEDDSQTFDIITNNETQEGIVILKKKVDFEHQNHYGIRAKVKNRHVDEKLMKYHAEASTTFIKVQVEDDDEPPVFLFPYYIFEIFEGSPHGSFVGVVSATDPDQRNSPIGYSVTRSKVFSIDDNGTIIATNPLDREISAWYNLSVTATEKYNVQQISAVPVYVQVLNINDHAPEFSEYYETFVCENAASGQVIQTISAVDRDESVEDHHFYFNLSVEDAKNSSFTIMDNQDNTAVILTNRTGFSLQEEPVFYISILIADNGIPSLTSTNTLTIYICDCDDNGSTQTCSNKDLMLSLGFRTEIIIAILISIMIIFGFIFLILGLKQQRKQTLFPEKGEDFRENIFRYDDEGGGEEDTEAFDIAELRSSTIMRERRTRKVPAAEIRSLYRQSLQVGPDSAIFRKFILEKLEEANTDPCAPPFDSLQTYAFEGTGSLAGSLSSLGSAVSDQDENYDYLNELGPRFKRLACMFGSAMQSNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J9H8</accession>
    <name>A0A9L0J9H8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106847956</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the immunoglobulin superfamily. CEA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J9H8"/>
    <dbReference type="Ensembl" id="ENSEAST00005058537.1">
        <property type="protein sequence ID" value="ENSEASP00005049023.1"/>
        <property type="gene ID" value="ENSEASG00005003392.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000263479"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990782">
        <property type="term" value="F:protein tyrosine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002682">
        <property type="term" value="P:regulation of immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20948">
        <property type="entry name" value="IgC2_CEACAM5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05740">
        <property type="entry name" value="IgI_hCEACAM_2_4_6_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd05774">
        <property type="entry name" value="IgV_CEACAM_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12087">
        <property type="entry name" value="TM_EGFR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000340">
        <property type="entry name" value="Carcinoembryonic antigen-related cell adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000517">
        <property type="entry name" value="Carcinoembryonic antigen-related cell adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000244">
        <property type="entry name" value="carcinoembryonic antigen-related cell adhesion molecule 16"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050831">
        <property type="entry name" value="CEA_cell_adhesion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44427:SF1">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44427">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040299518" description="Ig-like domain-containing protein" evidence="9">
        <location>
            <begin position="35"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="426"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="142"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="237"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="325"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="461"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="468"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005049023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="522" mass="57164" checksum="3E0518415AE31453" modified="2023-09-13" version="1">MELPIAPAGKGCVPWQGLLLAVLLSTFWNPPTTTQLTIELMPTNVAEGKDVIFLVRNLPGKLAAYGWYKGDKVDHHQQIASYKIGPVEITPGPVYSGRETIYSNGSLLFRNVTQEDTGYYTLQVINTSYQGEVGTGQLRVYPELNKPNITCNNSNPMEHKDPVVLTCEPETQDTTYLWSINNQSLLDSTWLELSLDNRTLTLLHITRNDTGPYECETRNPVSARRSDPFYLNVLYGPDTPIISPSDSYYHPGANLSLSCHAASNPPAQYSWLINGRPQQSTQELFIPNISANDSGSYACLVHNSVTGLNRTTVKTITISEPVAQPSIQASNTTVTEHQDPVVLTCLTNDTGISIQWFFNNQSLQLTERMKLSQDNSTLTIDPIRREDAGDYQCEVSNLVSASKSDLLRLDVKYDPTLGSSGLSAGAIGGIVIGVLAGVALIAALVYFLYIRKTGGASVQHDLTEHKPSASNHSQGHSDNSPNKMDEVTYSSLNFNVQESEKTTSASPPPSATETVYSEVKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JAG3</accession>
    <name>A0A9L0JAG3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein FAM234A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Protein ITFG3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">FAM234A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the FAM234 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014704322.1">
        <property type="nucleotide sequence ID" value="XM_014848836.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070341449.1">
        <property type="nucleotide sequence ID" value="XM_070485348.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070341450.1">
        <property type="nucleotide sequence ID" value="XM_070485349.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JAG3"/>
    <dbReference type="Ensembl" id="ENSEAST00005038225.1">
        <property type="protein sequence ID" value="ENSEASP00005046220.1"/>
        <property type="gene ID" value="ENSEASG00005017914.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106836158"/>
    <dbReference type="KEGG" id="eai:106836158"/>
    <dbReference type="CTD" id="83986"/>
    <dbReference type="GeneTree" id="ENSGT00530000063694"/>
    <dbReference type="OMA" id="FMFWGLM"/>
    <dbReference type="OrthoDB" id="6364780at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002632">
        <property type="entry name" value="Protein FAM234A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055409">
        <property type="entry name" value="Beta-prop_FAM234A_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045232">
        <property type="entry name" value="FAM234"/>
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    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21419:SF7">
        <property type="entry name" value="PROTEIN FAM234A"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF23727">
        <property type="entry name" value="Beta-prop_FAM234A_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="FAM234A/B beta-propeller" evidence="11">
        <location>
            <begin position="82"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00083714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF23727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005046220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="553" mass="60007" checksum="71B134ABA720409D" modified="2023-09-13" version="1">MTDTKDLEAEIHPLKNEDRKLQEDLENRVKKEDILTRTPPQSRLSRCRAVAFFLSLFICLLVVFVVSFIIPCPDRPASQGTWRIHYNTAVTYDFLATEDVNRDRIQDVLFLYKSTNSSNSFINQSCADEGFSSPCTFVAAVSGANGSVLWERPAAQDGALVECAVPQPRDSGASSACILVGRPSSLIAVDSFTGETLWSRPSSFGGNASVLRPLLQVPDVDADGAPDLLVLTREDGEVHGYVHSGSTGHQIGRRGSLGVDGTSGSLLHVTRAGAPYILLPCAGSLCGYSVKGLYEKVTGRDSPLKKDPRWEGMLNATTHRLLLHSSGAVRFLMVVPGKAGEDLLLVSSEACKLLDGQDLAPRWTLSAAQVLRKPVLGYYKPDTWAVVIENGTGTDRQILLLDLSSGAVLWSQVLPGLPGDPQSTSLPTADHRSAFFFWGLHELMGTNQMEPGDAQHSLYMFHPTLPSVLLELANVSANIIAFHAVLFEPSRHAACLLLTGPASPDVPGLVSVAKHKVRDLILSSRVVRLAEGGPDSDETVRDRFSRLRYRSEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JBL9</accession>
    <name>A0A9L0JBL9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Uteroglobin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Secretoglobin family 1A member 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106826817</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the secretoglobin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JBL9"/>
    <dbReference type="Ensembl" id="ENSEAST00005061417.1">
        <property type="protein sequence ID" value="ENSEASP00005050569.1"/>
        <property type="gene ID" value="ENSEASG00005025253.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155073"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019834">
        <property type="term" value="F:phospholipase A2 inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00633">
        <property type="entry name" value="Secretoglobin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.210.10:FF:000001">
        <property type="entry name" value="Uteroglobin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.210.10">
        <property type="entry name" value="Secretoglobin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016126">
        <property type="entry name" value="Secretoglobin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043215">
        <property type="entry name" value="Secretoglobin_1C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035960">
        <property type="entry name" value="Secretoglobin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000329">
        <property type="entry name" value="Uteroglobin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10136">
        <property type="entry name" value="SECRETOGLOBIN FAMILY 1 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10136:SF6">
        <property type="entry name" value="UTEROGLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01099">
        <property type="entry name" value="Uteroglobin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00486">
        <property type="entry name" value="UTEROGLOBIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00096">
        <property type="entry name" value="UTG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48201">
        <property type="entry name" value="Uteroglobin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51311">
        <property type="entry name" value="SCGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0593">Phospholipase A2 inhibitor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040153247" description="Uteroglobin" evidence="8">
        <location>
            <begin position="22"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="92" mass="9665" checksum="F8A040D3192E0CBC" modified="2023-09-13" version="1">MKLAIIVTLAILALCCSPASAEICQSFAGVIQSLFLGTPASFEAAVEPFINPDADMKAAATQLKTLVDLLPKNTKDSILKLMDKIAKSPLCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JFN6</accession>
    <name>A0A9L0JFN6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">KRAB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123275607</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JFN6"/>
    <dbReference type="Ensembl" id="ENSEAST00005041360.1">
        <property type="protein sequence ID" value="ENSEASP00005051148.1"/>
        <property type="gene ID" value="ENSEASG00005035846.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154734"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
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    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="150" mass="16981" checksum="7C111771E86D2214" modified="2023-09-13" version="1">MAAAARREPAQGTVTFEDVAVYFSWEEWDLLDEAQRCLYRDVMLENLTLITSLGCWCGAEDEAPEQSVSVERMSQVRFSKAGLSSQRAPPCEMCSPILKDILHLAENLETRRGQKLYRFGPCGKQLCFNANLHQHQKQDIGEKPFGINVD</sequence>
</entry>
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    <accession>A0A9L0JK03</accession>
    <name>A0A9L0JK03_EQUAS</name>
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        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
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                <person name="J."/>
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                <person name="A."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="14" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="2 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="gene ID" value="ENSEASG00005027763.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="eai:106842294"/>
    <dbReference type="GeneTree" id="ENSGT00940000153255"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15420">
        <property type="entry name" value="7tmA_OR2A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
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        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
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        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
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    <keyword evidence="4 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="101"/>
            <end position="119"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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        </location>
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        </location>
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            <begin position="40"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005053656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
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    <accession>A0A9L0JMK0</accession>
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        </recommendedName>
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        <name evidence="17" type="primary">GYG2</name>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Glycogenin participates in the glycogen biosynthetic process along with glycogen synthase and glycogen branching enzyme. It catalyzes the formation of a short alpha (1,4)-glucosyl chain covalently attached via a glucose 1-O-tyrosyl linkage to internal tyrosine residues and these chains act as primers for the elongation reaction catalyzed by glycogen synthase.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Self-glucosylating initiator of glycogen synthesis. It catalyzes the formation of a short alpha (1,4)-glucosyl chain covalently attached via a glucose 1-O-tyrosyl linkage to internal tyrosine residues and these chains act as primers for the elongation reaction catalyzed by glycogen synthase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-tyrosyl-[glycogenin] + UDP-alpha-D-glucose = alpha-D-glucosyl-L-tyrosyl-[glycogenin] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23360"/>
            <dbReference type="Rhea" id="RHEA-COMP:14604"/>
            <dbReference type="Rhea" id="RHEA-COMP:14605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:140573"/>
            <dbReference type="EC" id="2.4.1.186"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:23361"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>[1,4-alpha-D-glucosyl](n)-L-tyrosyl-[glycogenin] + UDP-alpha-D-glucose = [1,4-alpha-D-glucosyl](n+1)-L-tyrosyl-[glycogenin] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:56560"/>
            <dbReference type="Rhea" id="RHEA-COMP:14606"/>
            <dbReference type="Rhea" id="RHEA-COMP:14607"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:140574"/>
            <dbReference type="EC" id="2.4.1.186"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:56561"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the glycosyltransferase 8 family. Glycogenin subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.186" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0JMK0"/>
    <dbReference type="Ensembl" id="ENSEAST00005081990.1">
        <property type="protein sequence ID" value="ENSEASP00005053608.1"/>
        <property type="gene ID" value="ENSEASG00005018708.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161628"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008466">
        <property type="term" value="F:glycogenin glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02537">
        <property type="entry name" value="GT8_Glycogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000092">
        <property type="entry name" value="Glycogenin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="402"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="16">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="16">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005053608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="485" mass="52371" checksum="087CB6A188CDD5E2" modified="2023-09-13" version="1">PPRLSRAGRRRRRTPGGGGGGRWRPRPGPRGGRVGAGLPAGGQSPSSGPPSTRRAAAARGHRSAGGLEAAWARRGPGGTEAVWARRRPGNLHGPQETTPLLGPAGGRGVRPTHRAQARAGPRVPTARGAADHVGVVLSRVFDEVIEVNLIDSADYIHLAFLNRPELGVTLTKLHCWTLTRYSKCVFLDADTLVLSNIDELFERGEFSAAPDPGWPDCFNSGVFVFQPSLETHGLLLQHATDHGSFDGADQGLLNSFFSSWSTADIHKHLPFIYNLSSNTAYTYSPAFKQFGSSAKVVHFLGSRKPWNYKYNPQTGSLLEEGSELVTQHQASFLKVWWGIYHGSILPLYESIRDEQEHASPGRTACLGGVGAPCANSAPSAEGPCANSVPSARNLCADSAVGSSQAWPAEDPSETNVGVDETPPSPEGGHSEDVDLAVSISEMSLKEKVRELSPEEERRKWEEGHVDYMGQDAFARIQEKLDRFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JN24</accession>
    <name>A0A9L0JN24_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Frizzled-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">FZD1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor Fz/Smo family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044623410.1">
        <property type="nucleotide sequence ID" value="XM_044767475.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JN24"/>
    <dbReference type="Ensembl" id="ENSEAST00005051708.1">
        <property type="protein sequence ID" value="ENSEASP00005054619.1"/>
        <property type="gene ID" value="ENSEASG00005037063.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106832232"/>
    <dbReference type="KEGG" id="eai:106832232"/>
    <dbReference type="CTD" id="8321"/>
    <dbReference type="GeneTree" id="ENSGT00940000162584"/>
    <dbReference type="OMA" id="VPDHGYC"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030165">
        <property type="term" value="F:PDZ domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042813">
        <property type="term" value="F:Wnt receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017147">
        <property type="term" value="F:Wnt-protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035425">
        <property type="term" value="P:autocrine signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035567">
        <property type="term" value="P:non-canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045669">
        <property type="term" value="P:positive regulation of osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000739">
        <property type="term" value="P:regulation of mesenchymal stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd15247">
        <property type="entry name" value="7tmF_FZD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07465">
        <property type="entry name" value="CRD_FZ1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.2000.10:FF:000003">
        <property type="entry name" value="Frizzled class receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000029">
        <property type="entry name" value="Frizzled class receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.2000.10">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015526">
        <property type="entry name" value="Frizzled/SFRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000539">
        <property type="entry name" value="Frizzled/Smoothened_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020067">
        <property type="entry name" value="Frizzled_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036790">
        <property type="entry name" value="Frizzled_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309">
        <property type="entry name" value="FRIZZLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11309:SF81">
        <property type="entry name" value="FRIZZLED-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01534">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01392">
        <property type="entry name" value="Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00489">
        <property type="entry name" value="FRIZZLED"/>
    </dbReference>
    <dbReference type="SMART" id="SM00063">
        <property type="entry name" value="FRI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01330">
        <property type="entry name" value="Frizzled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63501">
        <property type="entry name" value="Frizzled cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50038">
        <property type="entry name" value="FZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="10" id="KW-0297">G-protein coupled receptor</keyword>
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    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="15" id="KW-0807">Transducer</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="328"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="410"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="455"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="494"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="541"/>
            <end position="564"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="606"/>
            <end position="628"/>
        </location>
    </feature>
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        <location>
            <begin position="116"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="20">
        <location>
            <begin position="323"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="82"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
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        <location>
            <begin position="121"/>
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    <feature type="disulfide bond" evidence="16">
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            <begin position="129"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="196"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008077"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005054619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="653" mass="71734" checksum="966D8398A26E2B3C" modified="2023-09-13" version="1">MRPERSRESMAEEEAPRKSRAAGGGASWELCAGALPAGTVAEGSGDAGGRRRPPAASGRLARRLLLLLWLLEAPLLLGVRAQAAGQGPGPGQQPPPPPQQQPSGQQYNGERGISIPDHGYCQPISIPLCTDIAYNQTIMPNLLGHTNQEDAGLEVHQFYPLVKVQCSAELKFFLCSMYAPVCTVLEQALPPCRSLCERARQGCEALMNKFGFQWPDTLKCEKFPVHGAGELCVGQNTSDKGTPTPSLLPEFWTSNPQHGGGGHRGGGFPGGAGGSERGKFSCPRALKVPSYLNYHFLGEKDCGAPCEPTKVYGLMYFGPEELRFSRTWIGIWSVLCCASTLFTVLTYLVDMRRFSYPERPIIFLSGCYTAVAVAYIAGFLLEDRVVCNDKFAEDGARTVAQGTKKEGCTILFMMLYFFSMASSIWWVILSLTWFLAAGMKWGHEAIEANSQYFHLAAWAVPAIKTITILALGQVDGDVLSGVCFVGLNNVDALRGFVLAPLFVYLFIGTSFLLAGFVSLFRIRTIMKHDGTKTEKLEKLMVRIGVFSVLYTVPATIVIACYFYEQAFRDQWERSWVAQSCKSYAIPCPHLQAGGGSPPHPPMSPDFTVFMIKYLMTLIVGITSGFWIWSGKTLNSWRKFYTRLTNSKQGETTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JPQ2</accession>
    <name>A0A9L0JPQ2_EQUAS</name>
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        <recommendedName>
            <fullName evidence="1">Proteasome assembly chaperone 2</fullName>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="3">Belongs to the PSMG2 family.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="PAC-like subunit"/>
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        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
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        <property type="entry name" value="Proteasome_assmbl_chp_2_euk"/>
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        <property type="entry name" value="PSMG2_sf"/>
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        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 2"/>
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        <property type="entry name" value="PAC2"/>
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    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00019186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005055194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="108" mass="11762" checksum="D92FDEE16C4ED1DE" modified="2023-09-13" version="1">MFVPCGESAPDLAAYTLLMPAVSVGNVGQLAVDLIISTLNMCKIGYFYTDCLVPMVGNNPYATAEENSAELSTNAEVYSLPSKKLVALQLRSIFINVIIFAFFKFVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JQ53</accession>
    <name>A0A9L0JQ53_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Anaphase promoting complex subunit 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ANAPC15</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="Guo"/>
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                <person name="Q."/>
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                <person name="X."/>
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                <person name="Q."/>
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                <person name="Z."/>
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                <person name="J."/>
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                <person name="R."/>
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                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
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                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the APC15 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JQ53"/>
    <dbReference type="Ensembl" id="ENSEAST00005053348.1">
        <property type="protein sequence ID" value="ENSEASP00005051285.1"/>
        <property type="gene ID" value="ENSEASG00005000567.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000938"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141198">
        <property type="term" value="P:protein branched polyubiquitination"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="ANAPHASE PROMOTING COMPLEX C SUBUNIT 15, PSEUDOGENE-RELATED"/>
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        <property type="entry name" value="ANAPC15"/>
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    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="93"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="32"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="111"/>
            <end position="170"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009618"/>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005051285.1"/>
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    <evidence type="ECO:0000313" key="8">
        <source>
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    <sequence length="170" mass="19193" checksum="97EC69F90D2B96AE" modified="2023-09-13" version="1">MTRLPAGAGNKRRPKTHASGVSRVLAGPSAQAERSASGSPRAAASRRGAMSTLFPSLFPRVTETLWFNLDRPCVEETELQQQEQQHQAWLQSIAEKDNNLVPIGKPASEHYDDEEEEDEDDDEDSEEDSEDDEDMQDMDEMNDYNESPDDGEVNEVDMEGNEQDQDQWMM</sequence>
</entry>
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    <accession>A0A9L0JSW5</accession>
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        <submittedName>
            <fullName evidence="13">ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase</fullName>
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        <name type="scientific">Equus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="11">Binds 1 Mn(2+) ion per subunit.</text>
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    <comment type="subcellular location">
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Glyco_transf_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000022">
        <property type="entry name" value="Histo-blood group ABO system transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005076">
        <property type="entry name" value="Glyco_trans_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10462">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10462:SF29">
        <property type="entry name" value="HISTO-BLOOD GROUP ABO SYSTEM TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03414">
        <property type="entry name" value="Glyco_transf_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="121"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-galactosamine</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>UDP-N-acetyl-alpha-D-galactosamine</name>
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    <feature type="binding site" evidence="10">
        <location>
            <begin position="211"/>
            <end position="213"/>
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        <ligand>
            <name>UDP-N-acetyl-alpha-D-galactosamine</name>
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        <location>
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        <ligand>
            <name>Mn(2+)</name>
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    </feature>
    <feature type="binding site" evidence="11">
        <location>
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        <ligand>
            <name>Mn(2+)</name>
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    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="233"/>
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        <ligand>
            <name>an alpha-L-fucosyl-(1-&gt;2)-beta-D-galactosyl derivative</name>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="303"/>
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        <ligand>
            <name>an alpha-L-fucosyl-(1-&gt;2)-beta-D-galactosyl derivative</name>
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    <feature type="binding site" evidence="10">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>an alpha-L-fucosyl-(1-&gt;2)-beta-D-galactosyl derivative</name>
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        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010413"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605076-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605076-2"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005056279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="354" mass="40935" checksum="B5A4429C46055C50" modified="2023-09-13" version="1">MAELLRTQARKPKCYLLRPVTFLLIMLVLVFFGAGFLSPRSPVLWSRRAGAGEAGRESVGLQVVKLSRMVYPQPKVLTPSRKDVLVLTPWLAPIIWEATFNTDILNEQFRLQNATIGITVFAIKKYVVFLKLFLETAEQHFMVGHRVNYYVFTDRPADVPHVPLGEGRQMVVLEVPASARWQDVSMSRMEMISSFCEQRFLREVDFLVCADVDMKFRDHVGVEILSQLFGTLHPGYYRASREAFPYERRPQSQAHIPRDQGDFYYMGAFFGGSVSEVYRLTKACHQAIVADRANGIEAVWHDESHLNRYLLDHKPTKVLSPEYMWDEKLLGWPAIMRKLRYVAVPKNHREIRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JYC0</accession>
    <name>A0A9L0JYC0_EQUAS</name>
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        <submittedName>
            <fullName evidence="5">C-type lectin domain family 3 member A</fullName>
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        <name evidence="5" type="primary">CLEC3A</name>
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        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_014701314.1">
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        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
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        <property type="entry name" value="CTDL_fold"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="TETRANECTIN-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CLECT"/>
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    <keyword evidence="1" id="KW-0430">Lectin</keyword>
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    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
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        <location>
            <begin position="23"/>
            <end position="196"/>
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        <location>
            <begin position="74"/>
            <end position="192"/>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="196" mass="22106" checksum="D6544892340326FB" modified="2023-09-13" version="1">MAKSGLVIYILVITLLLDQTTSHMSKVKARKHSKRRVKENDGDLKTQVEKLWREVNALKEMQALQTVCLRGTKFHKKCYLASEGSKHFHDANEDCISKGGTLVIPRNSDEINALRDYGKRSLPGVNDFWLGVNDMVTEGKFVDVNGIAISFLNWDNAQPNGGKRENCALLSQSAQGKWSDEDCRSSKRYICEFTIP</sequence>
</entry>
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    <accession>A0A9L0K2F7</accession>
    <name>A0A9L0K2F7_EQUAS</name>
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        <recommendedName>
            <fullName evidence="3">Cyclic AMP-responsive element-binding protein 3-like protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CREB3L4</name>
    </gene>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. ATF subfamily.</text>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14689">
        <property type="entry name" value="bZIP_CREB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000042">
        <property type="entry name" value="Cyclic AMP-responsive element-binding protein 3-like protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051381">
        <property type="entry name" value="CREB_ATF_subfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45996">
        <property type="entry name" value="AGAP001464-PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45996:SF2">
        <property type="entry name" value="CYCLIC AMP-RESPONSIVE ELEMENT-BINDING PROTEIN 3-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0834">Unfolded protein response</keyword>
    <feature type="domain" description="BZIP" evidence="18">
        <location>
            <begin position="217"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="82"/>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="17">
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            <begin position="352"/>
            <end position="378"/>
        </location>
    </feature>
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        <location>
            <begin position="242"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009050"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005059691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="393" mass="43033" checksum="918E64BFC39E03D4" modified="2023-09-13" version="1">MDFRTPELLDMWLEPPEDVFSTGSFLELGFHCPPPEVPVTRLQEQGKQGWESSGGLGCGLQESEPEDFLKLFIDPNEVYCSEASPGSDSGISEDPGHPDSPPASKAPSSPALYEVVYEAGALERMQGETGPAVEPISIQLDQRSPPLLMPDACMVSELHLDAHAHILPRAGTVNPVPPVTLLPCQTLFLTDEEKRLLGQEGVSLPSHLPLTKAEERVLKKVRRKIRNKQSAQDSRRRKKEYIDGLESRVAACSAQNQELQKKVQELERHNISLVTQLRQLQMLIAQTSNKAAQTSTCVLILLFSLALIILPSFSPFQGLPEAGPDDYQPHGVISRNILTHKDMTENLETPVVESRLGGPPGGVNGSTSTLLEKTGGKLGPSRRIRTVLHADEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K753</accession>
    <name>A0A9L0K753_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">BCL2 like 13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">BCL2L13</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Bcl-2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K753"/>
    <dbReference type="Ensembl" id="ENSEAST00005054363.1">
        <property type="protein sequence ID" value="ENSEASP00005061174.1"/>
        <property type="gene ID" value="ENSEASG00005035292.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015552"/>
    <dbReference type="OMA" id="MHEALQT"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045444">
        <property type="term" value="P:fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006119">
        <property type="term" value="P:oxidative phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.437.10:FF:000011">
        <property type="entry name" value="BCL2 like 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.437.10">
        <property type="entry name" value="Blc2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036834">
        <property type="entry name" value="Bcl-2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046371">
        <property type="entry name" value="Bcl-2_BH1-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002475">
        <property type="entry name" value="Bcl2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042398">
        <property type="entry name" value="BCL2L13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15758">
        <property type="entry name" value="BCL-2-LIKE PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15758:SF2">
        <property type="entry name" value="BCL-2-LIKE PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00452">
        <property type="entry name" value="Bcl-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56854">
        <property type="entry name" value="Bcl-2 inhibitors of programmed cell death"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50062">
        <property type="entry name" value="BCL2_FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="442"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="Bcl-2 Bcl-2 homology region 1-3" evidence="6">
        <location>
            <begin position="104"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="211"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="468" mass="51189" checksum="A8050D05D77D1458" modified="2023-09-13" version="1">MASSTTVPLGFHYETKYVVLSYLGLLSQEKLQEQHLSSPQGVQQDVTSQTLDQEVLLKVKAEIEEELKSLDKEISEAFTSTGFDRHTSPVFSPANPESSVEDCLAHLGEKVSQELKEPLQKALQMLLSQPVTYQAYRECTLETTVHANGWNKILVPLILLRQMLLELTRRGQEPLSALLQFGVTYLEDYAAEYIIQQGGWGTVFNLESEEEEYPGTVAEDSNDIYILPSDNSGQVSPPESPTVTTSWQSESLPVSLSASQSWHTESLPVSLGPESWQQIAMDPEEVKSLDSNGAGEKSENNSSNSDIVHVEKEEIPEGVEEAAVASVVLPSRELQEAFPEAPAPSLPHITATSLLEPREPDTEMIAVEKVSPATSVFVELDEEELKAATVEPIKLEEQVIPALEPTKTLLSEEERSVRKQSLTEVFSSAREEKAIPLSEGKAILLFGGAAAVAILAVAVGVALALRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K9J2</accession>
    <name>A0A9L0K9J2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Phosphoenolpyruvate carboxykinase [GTP], mitochondrial</fullName>
            <ecNumber evidence="6">4.1.1.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Phosphoenolpyruvate carboxykinase, cytosolic [GTP]</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Serine-protein kinase PCK1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">PCK2</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="23">Mitochondrial phosphoenolpyruvate carboxykinase that catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Can play an active role in glyceroneogenesis and gluconeogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-seryl-[protein] + GTP = O-phospho-L-seryl-[protein] + GDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:64020"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:64021"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>oxaloacetate + GTP = phosphoenolpyruvate + GDP + CO2</text>
            <dbReference type="Rhea" id="RHEA:10388"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.1.1.32"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:10389"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="21">
            <dbReference type="Rhea" id="RHEA:10390"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.32" evidence="6"/>
    <dbReference type="RefSeq" id="XP_014724097.1">
        <property type="nucleotide sequence ID" value="XM_014868611.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K9J2"/>
    <dbReference type="Ensembl" id="ENSEAST00005078487.1">
        <property type="protein sequence ID" value="ENSEASP00005062121.1"/>
        <property type="gene ID" value="ENSEASG00005011923.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106848606"/>
    <dbReference type="KEGG" id="eai:106848606"/>
    <dbReference type="CTD" id="5106"/>
    <dbReference type="GeneTree" id="ENSGT00390000001912"/>
    <dbReference type="OMA" id="SEHMFIT"/>
    <dbReference type="OrthoDB" id="5841594at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004613">
        <property type="term" value="F:phosphoenolpyruvate carboxykinase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071549">
        <property type="term" value="P:cellular response to dexamethasone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071333">
        <property type="term" value="P:cellular response to glucose stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032869">
        <property type="term" value="P:cellular response to insulin stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046327">
        <property type="term" value="P:glycerol biosynthetic process from pyruvate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070365">
        <property type="term" value="P:hepatocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006107">
        <property type="term" value="P:oxaloacetate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019543">
        <property type="term" value="P:propionate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00819">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.228.20:FF:000005">
        <property type="entry name" value="Phosphoenolpyruvate carboxykinase [GTP], mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.228.20:FF:000006">
        <property type="entry name" value="Phosphoenolpyruvate carboxykinase [GTP], mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.8.10:FF:000006">
        <property type="entry name" value="Phosphoenolpyruvate carboxykinase, cytosolic [GTP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.449.10:FF:000003">
        <property type="entry name" value="Phosphoenolpyruvate carboxykinase, cytosolic [GTP]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.228.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.449.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.8.10">
        <property type="entry name" value="Phosphoenolpyruvate Carboxykinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00452">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018091">
        <property type="entry name" value="PEP_carboxykin_GTP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013035">
        <property type="entry name" value="PEP_carboxykinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008209">
        <property type="entry name" value="PEP_carboxykinase_GTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035077">
        <property type="entry name" value="PEP_carboxykinase_GTP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035078">
        <property type="entry name" value="PEP_carboxykinase_GTP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008210">
        <property type="entry name" value="PEP_carboxykinase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003253">
        <property type="entry name" value="PRK04210.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11561">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11561:SF11">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE CARBOXYKINASE [GTP], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00821">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17297">
        <property type="entry name" value="PEPCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001348">
        <property type="entry name" value="PEP_carboxykinase_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF68923">
        <property type="entry name" value="PEP carboxykinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53795">
        <property type="entry name" value="PEP carboxykinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00505">
        <property type="entry name" value="PEPCK_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="7" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="16" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="18" id="KW-0456">Lyase</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Phosphoenolpyruvate carboxykinase GTP-utilising N-terminal" evidence="26">
        <location>
            <begin position="46"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoenolpyruvate carboxykinase C-terminal P-loop" evidence="25">
        <location>
            <begin position="278"/>
            <end position="635"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00040372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00042054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00049257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00056168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00069379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF00821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF17297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005062121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="640" mass="70701" checksum="D6679F446E5E4F02" modified="2023-09-13" version="1">MAAVYRPGLRLGWRGLSPWGWSSWRSLQTLRVFSGDLGQLPAGVRDFVEHSARLCQPEGIHICDGTEAENTAILTLLEEQGLIRKLPKYNNCWLARTDPKDVARVESKTVIVTPSQRDTVPLVAGGARGQLGNWMSPAEFQRAMDERFPGCMQGRTMYVLPFSMGPVGSPLSRIGVQLTDSAYVVASMRIMTRLGTPVLQALGDGDFVKGLHSVGQPLTGQGEPVSQWPCNPEKTLIGHVPDQREIVSFGSGYGGNSLLGKKCFALRIASRLARDEGWLAEHMLILGITNPAGKKRYVAAAFPSACGKTNMAMMRPALPDWKVECVGDDIAWMRFDSDGQLRAINPENGFFGVAPGTSATTNPNAMATIQSNTLFTNVAETSDGGVYWEGIDQPLSPGITVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIIDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGRYLEHWLSMEGRKGVRLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSAQETPIGLVPKEGALDLSGLGAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALEGRVRRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KA98</accession>
    <name>A0A9L0KA98_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Endosomal transmembrane epsin interactor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ENTREP3</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ENTREP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044613673.1">
        <property type="nucleotide sequence ID" value="XM_044757738.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0KA98"/>
    <dbReference type="Ensembl" id="ENSEAST00005071315.1">
        <property type="protein sequence ID" value="ENSEASP00005059337.1"/>
        <property type="gene ID" value="ENSEASG00005019271.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106828492"/>
    <dbReference type="CTD" id="10712"/>
    <dbReference type="GeneTree" id="ENSGT00530000063335"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050699">
        <property type="term" value="F:WW domain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030431">
        <property type="entry name" value="ENTREP1-3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17615:SF7">
        <property type="entry name" value="PROTEIN ENTREP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17615">
        <property type="entry name" value="PROTEIN FAM189A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="30"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="387"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="443"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="552"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="599"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="648"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="399"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="472"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="657"/>
            <end position="670"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005059337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="670" mass="71605" checksum="5C95A568276D6F89" modified="2023-09-13" version="1">MMPSPSDSSRSLTSRPSTRGLTHLRLHRPWLQALLTLGLAQVLLGILVVTFSMVASSVTTTESIKRSCPSWAGFSLAFSGVVGIVSWKRPFTLVISFFSLLSVLCVMLSMAGSVLSCKNAQLARDFRDCSMEGKVCVCCPSVPLLRPCPESGQELKIAPNSTCDEARGALKNLLFSVCGLTICAAIICTLSAIVCCIQIFSLDLVHTQLAPERSVSGPLGPLGCTSSPPAPLLHTMLDLEEFVPPVPPPPYYPPEYTCSSETDAQSITYNGSMDSPVPLYPTDCPPSYEAVMGLRGDSQATLFDPQLHDSSCICERVASIVDVSMDSGSLVLSAIGDLPGGSSPSEDSCLLELQGSVRSVDYVLFRSIQRSRAGYCLSLDCGLRGPFEDSPLPRRPPRAARSYSCSAPEAPPTLGTPTAARSCHRLEGWPPWVGPCFPELRRRVPRGGSQPAAAPPTRAPTRRFSDSSGSLTPPPGHRPPHPAPPPPLLLPRSHSDPGITTSSATADFRDLYTKVLEEEAASVSSADTGLCSEACLFRLARCPSPKLLRARSAEKRRPVPTFQKVPLPSGPAPAHSLGDLKGSWPGRGLVTRFLQMSRKAPDPSGIGAHGHKQVPRSPWGRPGRESLHLRSCGDLSSGSSLRRLLSARRLERGTRPHSLSLNGGSRETGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KAA9</accession>
    <name>A0A9L0KAA9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">IGF like family member 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106845147</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IGFL family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KAA9"/>
    <dbReference type="Ensembl" id="ENSEAST00005073756.1">
        <property type="protein sequence ID" value="ENSEASP00005062299.1"/>
        <property type="gene ID" value="ENSEASG00005032964.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009557"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
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    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032744">
        <property type="entry name" value="IGFL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34827:SF2">
        <property type="entry name" value="INSULIN GROWTH FACTOR-LIKE FAMILY MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34827">
        <property type="entry name" value="INSULIN GROWTH FACTOR-LIKE FAMILY MEMBER 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14653">
        <property type="entry name" value="IGFL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="14"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="16"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005062299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="16671" checksum="E3B356FDB73DA3F3" modified="2023-09-13" version="1">MPCSPLPPPCLTQAWGTGGAKGTGAPGDTTIGEDPEGSQPTAMAPRGRIVAVSAAVCILTLLCSHGAPVSPMGAHLMLCQPHLTCGDKFYDPLQHCCYDDAVVPLGRTQKCGNCTFRVCFEQCCFWSLRPLESFVVKVKGQSCSLALSPDDRLCRSVG</sequence>
</entry>
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    <name>A0A9L0KBH3_EQUAS</name>
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        <recommendedName>
            <fullName evidence="3">Immunoglobulin V-set domain-containing protein</fullName>
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            <taxon>Equus</taxon>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Yin"/>
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                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
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                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0KBH3"/>
    <dbReference type="Ensembl" id="ENSEAST00005074228.1">
        <property type="protein sequence ID" value="ENSEASP00005062725.1"/>
        <property type="gene ID" value="ENSEASG00005031529.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163545"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050413">
        <property type="entry name" value="TCR_beta_variable"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268:SF28">
        <property type="entry name" value="T CELL RECEPTOR BETA VARIABLE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23268">
        <property type="entry name" value="T-CELL RECEPTOR BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0391">Immunity</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Immunoglobulin V-set" evidence="3">
        <location>
            <begin position="19"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005062725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="163" mass="18671" checksum="8D1303C881A407F2" modified="2023-09-13" version="1">MDGGITQRPKYLIREERRDVTLECEQSLDYDYMCWYRQDPGQGLRLIYYSQVLKAVEKGDVSEGYRASREEKAFFPLTVTSTRKNQTALYLCASSRDTVKHCHLLFVHKCVPSPTSHQVAGLFWTQYLSLLAFFHLNQAPKAAASINMLCGMAGLFSDPLSWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KDM2</accession>
    <name>A0A9L0KDM2_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Late cornified envelope 3B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LCE3B</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
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            <taxon>Mammalia</taxon>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="C."/>
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                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LCE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KDM2"/>
    <dbReference type="Ensembl" id="ENSEAST00005059683.1">
        <property type="protein sequence ID" value="ENSEASP00005062939.1"/>
        <property type="gene ID" value="ENSEASG00005028563.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165404"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
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    <dbReference type="GO" id="GO:0050829">
        <property type="term" value="P:defense response to Gram-negative bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031424">
        <property type="term" value="P:keratinization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
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    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014711126.1">
        <property type="nucleotide sequence ID" value="XM_014855640.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0KM84"/>
    <dbReference type="Ensembl" id="ENSEAST00005082952.1">
        <property type="protein sequence ID" value="ENSEASP00005063116.1"/>
        <property type="gene ID" value="ENSEASG00005031367.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106840290"/>
    <dbReference type="KEGG" id="eai:106840290"/>
    <dbReference type="CTD" id="643"/>
    <dbReference type="GeneTree" id="ENSGT01050000244848"/>
    <dbReference type="OMA" id="FTKNCLL"/>
    <dbReference type="OrthoDB" id="9818824at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019957">
        <property type="term" value="F:C-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016493">
        <property type="term" value="F:C-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016494">
        <property type="term" value="F:C-X-C chemokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042113">
        <property type="term" value="P:B cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019722">
        <property type="term" value="P:calcium-mediated signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030595">
        <property type="term" value="P:leukocyte chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048535">
        <property type="term" value="P:lymph node development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032467">
        <property type="term" value="P:positive regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007204">
        <property type="term" value="P:positive regulation of cytosolic calcium ion concentration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd15181">
        <property type="entry name" value="7tmA_CXCR5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000143">
        <property type="entry name" value="C-X-C chemokine receptor type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050119">
        <property type="entry name" value="CCR1-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001053">
        <property type="entry name" value="Chemokine_CXCR5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489:SF618">
        <property type="entry name" value="C-X-C CHEMOKINE RECEPTOR TYPE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10489">
        <property type="entry name" value="CELL ADHESION MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00564">
        <property type="entry name" value="CXCCHMKINER5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0075">B-cell activation</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="301"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="70"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00069929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005063116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="374" mass="42055" checksum="842194F0A6157C93" modified="2023-09-13" version="1">MNYPLTLDMDLTNYNLEDLYKELGSYNDSSEPPVVENHLCSTVEGPLLTSFKAVFMPMAYGLIFLLGMMGNILVLVILERHRQTRNSTETFLFHLALADLLLVLILPFAVAEGFVGWVLGTGLCKTVIALHKINFYCSSLLLACIAVDRYLAIVHSVHAYRHRRLHSIHITCITIWLAGLLFALPELLFAKVSQPHHNGSLPRCTFSQENQAETNAWFTSRFLYHIGGFLLPMLVMGWCYVGVVHRLCQAQRRPQRQKAVRVAILVTSVFFLCWSPYHIIIFLDTLARLKAMVNSCDQNGYLSVAITLSEFLGLAHCCLNPMLYTFVGVKFRSDLSRLLTKLGCAGPASLCQFLPSWHKSSLSESENATSLTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KRT4</accession>
    <name>A0A8C4KRT4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial disaggregase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Suppressor of potassium transport defect 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CLPB</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Functions as a regulatory ATPase and participates in secretion/protein trafficking process. Has ATP-dependent protein disaggregase activity and is required to maintain the solubility of key mitochondrial proteins. Involved in mitochondrial-mediated antiviral innate immunity, activates RIG-I-mediated signal transduction and production of IFNB1 and pro-inflammatory cytokine IL6. Plays a role in granulocyte differentiation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homododecamer when substrate-bound; the homododecamer consists of 2 homohexamers stacked head-to-head via ANK repeat-mediated interactions. The active substrate-bound form is likely to exist in a dynamic equilibrium between homohexamers and homododecamers. Homotetradecamer in the unbound state which is remodeled upon substrate binding into the homododecamer. Interacts with PHB and PHB2. Interacts with MAVS; the interaction is enhanced by Sendai virus infection.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ClpA/ClpB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KRT4"/>
    <dbReference type="Ensembl" id="ENSEAST00005000633.2">
        <property type="protein sequence ID" value="ENSEASP00005000528.2"/>
        <property type="gene ID" value="ENSEASG00005000496.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012961"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19499">
        <property type="entry name" value="RecA-like_ClpB_Hsp104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000040">
        <property type="entry name" value="caseinolytic peptidase B protein homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000203">
        <property type="entry name" value="caseinolytic peptidase B protein homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000641">
        <property type="entry name" value="caseinolytic peptidase B protein homolog isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050130">
        <property type="entry name" value="ClpA_ClpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11638:SF93">
        <property type="entry name" value="MITOCHONDRIAL DISAGGREGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="ClpB_D2-small"/>
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        <property type="entry name" value="Ankyrin repeat"/>
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
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    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9 16" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
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            <begin position="132"/>
            <end position="164"/>
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            <begin position="342"/>
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    <feature type="domain" description="Clp ATPase C-terminal" evidence="19">
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            <begin position="540"/>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KTL4</accession>
    <name>A0A8C4KTL4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">La ribonucleoprotein 1, translational regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LARP1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the LARP family.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSEASP00005001274.2"/>
        <property type="gene ID" value="ENSEASG00005001070.2"/>
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        <property type="component" value="Chromosome 9"/>
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        <property type="term" value="C:cytoplasmic stress granule"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:TORC1 complex"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:eukaryotic initiation factor 4E binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:ribosomal small subunit binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:RNA 7-methylguanosine cap binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0008494">
        <property type="term" value="F:translation activator activity"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0008283">
        <property type="term" value="P:cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072752">
        <property type="term" value="P:cellular response to rapamycin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048255">
        <property type="term" value="P:mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045947">
        <property type="term" value="P:negative regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045070">
        <property type="term" value="P:positive regulation of viral genome replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990928">
        <property type="term" value="P:response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038202">
        <property type="term" value="P:TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="LARP_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="la-related protein 1B isoform X2"/>
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        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
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        <property type="entry name" value="La_HTH"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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        <property type="entry name" value="WH_DNA-bd_sf"/>
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    <dbReference type="PANTHER" id="PTHR22792:SF51">
        <property type="entry name" value="LA-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22792">
        <property type="entry name" value="LUPUS LA PROTEIN-RELATED"/>
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        <property type="entry name" value="La"/>
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    <dbReference type="Pfam" id="PF21071">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="DM15"/>
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50961">
        <property type="entry name" value="HTH_LA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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            <begin position="406"/>
            <end position="496"/>
        </location>
    </feature>
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        <location>
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            <begin position="824"/>
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        </location>
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            <begin position="1037"/>
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    </feature>
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            <begin position="7"/>
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            <begin position="63"/>
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    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="4">
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    <feature type="compositionally biased region" description="Polar residues" evidence="4">
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            <begin position="1095"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00061352"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00332"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001274.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1107" mass="124289" checksum="7B1E95C8BFFCDEA4" modified="2023-09-13" version="2">MATQVEPLLPGGAPLLQAEEHGGLVRKKPPPPPEGKGEPGANDVGGGEPDGGARRPRPPCVKPHKEGTGQLERESPRPPQPPGAESLTISDGEEGGGGEPGAGGGAAGAAGAGRRDFVEAPPPKVNPWTKNALPPVLATVNGQSPPEHSAPAKVVRAAVPKQRKGSKVGDFGDAINWPTPGEIAHKSVQPQSHKSQPARKLPPKKDMKEQEKGEGSDSKESPRTKSDESGEEKNGDEDCQRGGQKKKGNKHKWVPLQIDMKPEVPREKLVSRPARPPEPRHTSAIRGEIKGSEPPTYVSVPVAPPTPAWQPETKPEPAWHDQDETSSVKSDGAGGARASFRGRGRGRGRGRGRGRGGTRTPVSPVAHFDYQFGYRKFDGAEGPRTPKYMNNITYYFDNVSSTELYSVDQELLKDYIKRQIEYYFSVDNLERDFFLRRKMDADGFLPITLIASFHRVQALTTDISLIFAALKDSKVVEMVDEKVRRREEPEKWPLPGPPIVDYSQTDFSQLLNCPEFVPRQHYQKETESAPGSPRAVTPVPTKTEEVSNLKTLPKGLSASLPDLDSENWIEVKKRPRPSPSRPKKSEEPRFLHPASLPQQLPSQQLLSKDQDEQEELDFLFDEEMEQMDGRKNTFTAWSDEDSDYEIDDRDVNKILIVTQTPPYMRRHPGGDRTGNHTSRAKMSAELAKVINDGLFYYEQDLWTEKFEPEYSQIKQEVENFKKVNMISREQFDTLTPEPPVDPNQEVPPGPPRFQQVPTDALANKLFGAPEPSTIARSLPTTVPESPNYRNARTPRTPRTPQLKDSSQTSRFYPVVKEGRTLDAKMPRKRKTRHSSNPPLESHVGWVMDSREHRPRTASISSSPSEGTPAVGSYGCTPQSLPKFQHPSHELLKENGFTQHVYHKYRRRCLNERKRLGIGQSQEMNTLFRFWSFFLRDHFNKKMYEEFKQLALEDAKEGYRYGLECLFRYYSYGLEKKFRLDIFKDFQEETVKDYEAGQLYGLEKFWAFLKYSKAKNLDINPKLQEYLGKFRRLEDFRVDPPMGEEGNHKRHPVAAGGGSGEGRKRCPSQSSSRPATMISQPPTPPTGQPIREDAKWTSQHSDTQTLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KYV4</accession>
    <name>A0A8C4KYV4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Sushi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the receptors of complement activation (RCA) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005003684.2">
        <property type="protein sequence ID" value="ENSEASP00005003359.2"/>
        <property type="gene ID" value="ENSEASG00005002594.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160375"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006958">
        <property type="term" value="P:complement activation, classical pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030449">
        <property type="term" value="P:regulation of complement activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903659">
        <property type="term" value="P:regulation of complement-dependent cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000070">
        <property type="entry name" value="Complement C3d receptor 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000038">
        <property type="entry name" value="Complement component receptor type 1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000044">
        <property type="entry name" value="Complement component receptor type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000008">
        <property type="entry name" value="Complement receptor type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000033">
        <property type="entry name" value="Complement receptor type 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000014">
        <property type="entry name" value="Membrane cofactor protein"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050350">
        <property type="entry name" value="Compl-Cell_Adhes-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325">
        <property type="entry name" value="COMPLEMENT COMPONENT-RELATED SUSHI DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325:SF545">
        <property type="entry name" value="COMPLEMENT RECEPTOR TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="23"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="23"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0180">Complement pathway</keyword>
    <keyword evidence="10 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0391">Immunity</keyword>
    <keyword evidence="3" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0768">Sushi</keyword>
    <keyword evidence="13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040356876" description="Sushi domain-containing protein" evidence="14">
        <location>
            <begin position="41"/>
            <end position="1586"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1521"/>
            <end position="1545"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="40"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="101"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="163"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="235"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="294"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="355"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="418"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="490"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="551"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="613"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="685"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="744"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="805"/>
            <end position="867"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="868"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="943"/>
            <end position="1003"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="1004"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="1066"/>
            <end position="1136"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="1138"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="1197"/>
            <end position="1257"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="1258"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="1321"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="1395"/>
            <end position="1455"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="15">
        <location>
            <begin position="1456"/>
            <end position="1516"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="42"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="265"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="296"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="325"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="492"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="715"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="746"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="775"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="945"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1168"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1199"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1228"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1397"/>
            <end position="1440"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1426"/>
            <end position="1453"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1458"/>
            <end position="1501"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="1487"/>
            <end position="1514"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003359.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1586" mass="174361" checksum="AF455FEF5F597B5C" modified="2023-09-13" version="2">MGASAARSPEPLPRLAPALSCCCGALLAVLVLLALPAAWGQCKAPEWLPFAKPTTLTGENEFPIGTSLKYKCRPGYYQREFSITCLPNSVWSTAENICKRKQCRSPPEPTNGKMVVNGDIQFGSTVNYLCNEGYRLIGHSSTTCIISGNTVIWDNEAPVCESIPCGPPPAIANGDFISTNREYFPYGTVVTYYCNPRERRIKLFRLIGQPSIYCTSLDNQVGVWSGPPPQCIVPNTCTPPDVENGIRVSENRSFFPLNEIVEFRCQPGFVMKGPSSVRCQAQNKWVPELPSCSRACQPPPEIPYGKHTPSNKDNFSPGQEVFYSCEPGYDLRGAASLSCTPQGDWRPEAPRCAGKSCADFLDQLPNGRILFPLNLQLGAKVSFVCDEGFQLKGNSASHCILVGMESLWNSSVPVCEQILCPNPPDILNGRHTGKPLEVFPFGKEVTYTCDPHPYRGMTYNLTGESTIRCTSDSQGNGIWSSPAPRCVLPGYCNSPDHFQFAKLKIQTNESVFPIGTSLKYECRPEFYRRSFSITCLENLTWSNAKDVCKRRSCKTPPDPMNGVVHVDTDTQFGSRINYSCNRGYRLIGQSSAKCIISGNTVSWETEPPICERIPCGPPPAIANGDFISTNREYFPYGTVVTYYCNPRERRIKLFHLIGEPSIYCTSLDNQVGVWSGPPPQCIVPNTCTPPDVENGIRVSENRSFFPLNEIVEFRCQPGFVMKGPSSVRCQAQNKWVPELPSCSRVCQPPPKILHGRHITSNKDEFSPGQEVFYSCEPGYDLRGAASLRCTPQGDWSPEAPRCAVKSCADFIDQLPNGRILLPLNFQLGAKVSFICDEGFHLKGSSASYCILVGMESLWNNSVPVCEQIFCPNPPAILNGNHTGTSLGDIPYGKEISYTCDLHPDRGMTFNLIGERTIRCTSDSQGNGIWSSPAPRCELSGPAGYCKAPEQFPFAKPTTLTDESEFPIGTSLNYECSPGYFENMFSITCLENLVWSSAEDICRRKSCGTPPEPFNGMVHINTDTQFGSTVNYSCNEGYRLIGSLSAACLLSGNNVTWDKEAPLCESISCEPPPAISNGDYYSSNGNVFKYATVVTYRCLTGPDGEKLFDLVGEQSIFCTSKDNQLGVWSSPPPQCVSASKCTTPEVENGIRLSGNKSLFSLSEIVRFRCQPGFVMKGSNVVQCWANNTWVPELPSCSRACQLPPEIPHGKHTPNNKDDFSPGQEVFYSCEPGYDLRGAASLRCTPQGDWSPEAPRCAVKSCADFLDQLPNGRVLFPLNFQLGAEVSFVCDEGFRLKGSSTSRCVLVGMISLWNSSVPVCEQIFCSNPPAILNGRHTGTSLGDIPYGKEITYTCDPHPVRGMTFNLVGESTIRCTSDSQGNGFWSSPAPRCEPSGSAACPHPPKIHNGHKIGGRASPYLPGMTVTYTCDPGYLLVGNAFIFCTYLGTWSQSDHYCKEVKCSLPQFMNGIQKEMKIKNIYHYGDNVTLECEDGYSLKGSPQSQCQADNTWDPPLAICTSRPRDALIVGIFFGMIFFIVPIIISCWMVLKRKKGNNTDGKSKEVIIHLHPQQGNCVQPQSLQTNQENGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KZ35</accession>
    <name>A0A8C4KZ35_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">EGF-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KZ35"/>
    <dbReference type="Ensembl" id="ENSEAST00005000306.2">
        <property type="protein sequence ID" value="ENSEASP00005000227.2"/>
        <property type="gene ID" value="ENSEASG00005000255.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162597"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000216">
        <property type="entry name" value="Adhesion G protein-coupled receptor E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000269">
        <property type="entry name" value="Adhesion G protein-coupled receptor E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000382">
        <property type="entry name" value="Adhesion G protein-coupled receptor E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051586">
        <property type="entry name" value="PKC-binding_NELL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24042:SF5">
        <property type="entry name" value="EGF-LIKE CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24042">
        <property type="entry name" value="NEL HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1 5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="71"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="7">
        <location>
            <begin position="123"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000227.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="255" mass="26830" checksum="FFFB8FA833513F88" modified="2023-09-13" version="2">MCPQAQPESGDERTISRISGVDGSSPAVSRGATRELSSVPSLWQSQAGSSVSALWELSPLGGDPLPCCVPDINECGPPLAVSCGKFADCQNVDGSYYCTCAPGYGLVSGATTFRNESENTCQDVDECQQKPRICKGRSVCINTLGSYTCTCLPGLELNPKTRIYVNECASGHNPCHSSTHCLSVLGSYKCCCHPGWKPVPGSPEGPDSTVCAGPELSGCTPGDPHPEQLLTYSMAPTRPQQHEGWGCLLGQVLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KZL5</accession>
    <name>A0A8C4KZL5_EQUAS</name>
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        <submittedName>
            <fullName evidence="15">Neuromedin U receptor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NMUR2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Receptor for the neuromedin-U and neuromedin-S neuropeptides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014702431.2">
        <property type="nucleotide sequence ID" value="XM_014846945.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KZL5"/>
    <dbReference type="Ensembl" id="ENSEAST00005001581.2">
        <property type="protein sequence ID" value="ENSEASP00005001402.2"/>
        <property type="gene ID" value="ENSEASG00005001147.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106834928"/>
    <dbReference type="KEGG" id="eai:106834928"/>
    <dbReference type="CTD" id="56923"/>
    <dbReference type="GeneTree" id="ENSGT01120000271823"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005229">
        <property type="term" value="F:intracellularly calcium-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042924">
        <property type="term" value="F:neuromedin U binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001607">
        <property type="term" value="F:neuromedin U receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050482">
        <property type="term" value="P:arachidonate secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007625">
        <property type="term" value="P:grooming behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002023">
        <property type="term" value="P:reduction of food intake in response to dietary excess"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048265">
        <property type="term" value="P:response to pain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000214">
        <property type="entry name" value="Neuromedin U receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005390">
        <property type="entry name" value="NeuromedU_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005392">
        <property type="entry name" value="NeuromedU_rcpt_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045561">
        <property type="entry name" value="NMU-R2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24243:SF14">
        <property type="entry name" value="NEUROMEDIN-U RECEPTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19285">
        <property type="entry name" value="NmU-R2_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01565">
        <property type="entry name" value="NEUROMEDINUR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01567">
        <property type="entry name" value="NEUROMEDNU2R"/>
    </dbReference>
    <dbReference type="SMART" id="SM01381">
        <property type="entry name" value="7TM_GPCR_Srsx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="215"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="269"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="306"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="62"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001402.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="403" mass="45953" checksum="88FE46112C0F9534" modified="2023-09-13" version="2">MSVMEKHENVSWIHQQELEDPLKKYLNSTEDYLTFLCGPPRSHLSLPVTVVYALIFVVGVVGNLLVCLVILRHQTMKTPTNYYLFSLAVSDLLVLLLGMPLEVYEMWRNYPFLFGSVGCYFKTALFETVCFASILSVTTVSVERYVAIVHPFRAKLESTRRRALKIIGILWGLSFLFSLPNTSIHGIKLHYFPNGSLVPGSATCTVIKPMWIYNFIIQITSFLFYILPMTVISILYYLMGLRLRKEQHLEADEVTANVQRPSRKSVTKMLFVLVLVFAICWIPFHVDRLFFSFVEEWTESLAAVFNLIHVVSGVFFYLSSAVNPIIYNVLSHRFRAAFRNVISPSCKQWHSQHHPQGPPAQRNVFLTECHLVELTEDAGPQFPCQSSLCSYHLPAALCTGQAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L4D4</accession>
    <name>A0A8C4L4D4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Exostosin-like 1</fullName>
            <ecNumber evidence="4">2.4.1.224</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Exostosin-L</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Multiple exostosis-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">EXTL1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Glycosyltransferase required for the biosynthesis of heparan-sulfate (HS). Transfers N-acetyl-alpha-D-glucosamine to the nascent HS chain (GlcNAcT-II activity). Appears to lack GlcNAcT I and GlcAT-II activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>3-O-{[(1-&gt;4)-beta-D-GlcA-(1-&gt;4)-alpha-D-GlcNAc](n)-(1-&gt;4)-beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl}-L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-{alpha-D-GlcNAc-[(1-&gt;4)-beta-D-GlcA-(1-&gt;4)-alpha-D-GlcNAc](n)-(1-&gt;4)-beta-D-GlcA-(1-&gt;3)-beta-D-Gal-(1-&gt;3)-beta-D-Gal-(1-&gt;4)-beta-D-Xyl}-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16213"/>
            <dbReference type="Rhea" id="RHEA-COMP:12621"/>
            <dbReference type="Rhea" id="RHEA-COMP:12623"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132415"/>
            <dbReference type="ChEBI" id="CHEBI:132416"/>
            <dbReference type="EC" id="2.4.1.224"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 47 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.224" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4L4D4"/>
    <dbReference type="Ensembl" id="ENSEAST00005005994.2">
        <property type="protein sequence ID" value="ENSEASP00005005487.2"/>
        <property type="gene ID" value="ENSEASG00005004055.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106839934"/>
    <dbReference type="KEGG" id="eai:106839934"/>
    <dbReference type="CTD" id="2134"/>
    <dbReference type="GeneTree" id="ENSGT00940000161960"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050508">
        <property type="term" value="F:glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015012">
        <property type="term" value="P:heparan sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000109">
        <property type="entry name" value="Exostosin like glycosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004263">
        <property type="entry name" value="Exostosin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040911">
        <property type="entry name" value="Exostosin_GT47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015338">
        <property type="entry name" value="GT64_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48261">
        <property type="entry name" value="ACETYLGLUCOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48261:SF3">
        <property type="entry name" value="EXOSTOSIN GLYCOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03016">
        <property type="entry name" value="Exostosin_GT47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09258">
        <property type="entry name" value="Glyco_transf_64"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Exostosin GT47" evidence="21">
        <location>
            <begin position="94"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase 64" evidence="22">
        <location>
            <begin position="417"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="605"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00074809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00082373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00083239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF03016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF09258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005487.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="675" mass="74195" checksum="01FCF0F54B8C3460" modified="2023-09-13" version="2">MQSWRRKALLLALSASWLLVLLGAFPLLRLAVPTRPRAGASQGWPRWLDAELLQSFSQPGELLEDAPELPQGPRGGSCDWGACFDASKCRGGGLKVFVYSAAGPVSETHRRILASIEGSRYHTSNAAEACLLLLPLTRDSSAGECSPVPPQWNGGRNHLVLSLHPAPCPRTFQLGQAIVAEASPTVDTFRHGFDVALPLLPEAHALRGGAPGQLRQHSPHPRVALLALAEERGGWHTAGTNFSACPWDGHCEQDHGPEQTHPGATLPNATFCLIPGHRPDASHFLQALQAGCIPVLLSPRWELPFSEVIDWTKAAIVTDKRLPLQVLAALQQMPLTRVLALRQQTQFLWDAYFSSVEKVIHTTLEIIQDRIFGASAHPSLLWNSPPGALLALSTFSTSPSDFPFYHVQQGSRPVGRFSALIWVGAPGKPPLKLIQAVAGSQHCAQILVLWSSEKPPPPRWPETAVPLTVSEGHRKVSDRFFPYNAISTDAILSLDAHSSLSTSEVDFAFVVWQSFPERMVGFLTWSHFWDEALGGWGYTAERANEFSMVLTSAAFYHRYYHTLFTHSLPEALRALADEAPTCVDVLMNFLVAAVTKLPPIKVPYRKQHQEARPPLAPEGRGPRPEPQPPVRNCINQMAAGFGHMPLVSSRLRLDPVLFKDSVSVLRKKYRSLEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L5Z1</accession>
    <name>A0A8C4L5Z1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peroxisomal biogenesis factor 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Peroxin-19</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Peroxisomal farnesylated protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">PEX19</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Necessary for early peroxisomal biogenesis. Acts both as a cytosolic chaperone and as an import receptor for peroxisomal membrane proteins (PMPs). Binds and stabilizes newly synthesized PMPs in the cytoplasm by interacting with their hydrophobic membrane-spanning domains, and targets them to the peroxisome membrane by binding to the integral membrane protein PEX3. Excludes CDKN2A from the nucleus and prevents its interaction with MDM2, which results in active degradation of TP53.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with a broad range of peroxisomal membrane proteins, including PEX3, PEX10, PEX11A, PEX11B, PEX12, PEX13, PEX14 and PEX16, PXMP2/PMP22, PXMP4/PMP24, SLC25A17/PMP34, ABCD1/ALDP, ABCD2/ALDRP, and ABCD3/PMP70. Also interacts with the tumor suppressor CDKN2A/p19ARF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the peroxin-19 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L5Z1"/>
    <dbReference type="Ensembl" id="ENSEAST00005003506.2">
        <property type="protein sequence ID" value="ENSEASP00005003205.2"/>
        <property type="gene ID" value="ENSEASG00005002453.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010993"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036105">
        <property type="term" value="F:peroxisome membrane class-1 targeting sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140597">
        <property type="term" value="F:protein carrier chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045046">
        <property type="term" value="P:protein import into peroxisome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006625">
        <property type="term" value="P:protein targeting to peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.900:FF:000001">
        <property type="entry name" value="Putative peroxisomal biogenesis factor 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.900">
        <property type="entry name" value="Pex19, mPTS binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006708">
        <property type="entry name" value="Pex19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038322">
        <property type="entry name" value="Pex19_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12774">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12774:SF2">
        <property type="entry name" value="PEROXISOMAL BIOGENESIS FACTOR 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04614">
        <property type="entry name" value="Pex19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="9" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0636">Prenylation</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="80"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00032710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003205.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="354" mass="38217" checksum="EAC4194CAF44B000" modified="2023-09-13" version="2">MVPGERGGKNSELPVPLKMAVPQRPTIPLVGRRGGDGVGAGAVESHLLRQVGGNKMAAAEDGCGVGADTDQELEELLESALDDFDKAKPSPAPPPTTTAPDASGPQKRSPGDTAKDALFASQEKFFQELFDSELASQATAEFEKAMKELAEEEPHLVEQFQKLSEAAGRVGSDATSQQEFTSCLKETLSGLAKNATDLQNSGMSEEELTKAMEGLGMDEGDGEGNILPIMQSIMQNLLSKDVLYPSLKEITEKYPEWLQSHQESLPPEQFEKYQEQHSVMGKICEQFEAETPTDSEATQKARFEMVLDLMQQLQDLGHPPKELAGEMPPGLNFDLDALNLSGPPGANGEQCLIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L619</accession>
    <name>A0A8C4L619_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Transcription factor IIIB 90 kDa subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">B-related factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">BRF1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="13">TFIIIB comprises at least the TATA-binding protein (TBP) and the B-related factor 1 (BRF1/TFIIIB90). Interacts with BDP1. Interacts with MAF1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFIIB family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L619"/>
    <dbReference type="Ensembl" id="ENSEAST00005006767.2">
        <property type="protein sequence ID" value="ENSEASP00005006184.2"/>
        <property type="gene ID" value="ENSEASG00005004538.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010349"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000126">
        <property type="term" value="C:transcription factor TFIIIB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="C:transcription preinitiation complex"/>
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                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Heterodimer with B2M (beta-2-microglobulin). Interacts with saposin C.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L6I7"/>
    <dbReference type="Ensembl" id="ENSEAST00005004987.2">
        <property type="protein sequence ID" value="ENSEASP00005004544.2"/>
        <property type="gene ID" value="ENSEASG00005003453.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01120000271825"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030883">
        <property type="term" value="F:endogenous lipid antigen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030884">
        <property type="term" value="F:exogenous lipid antigen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071723">
        <property type="term" value="F:lipopeptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048006">
        <property type="term" value="P:antigen processing and presentation, endogenous lipid antigen via MHC class Ib"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048007">
        <property type="term" value="P:antigen processing and presentation, exogenous lipid antigen via MHC class Ib"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001916">
        <property type="term" value="P:positive regulation of T cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21029">
        <property type="entry name" value="IgC1_CD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000254">
        <property type="entry name" value="Antigen-presenting glycoprotein CD1d1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.500.10:FF:000002">
        <property type="entry name" value="Antigen-presenting glycoprotein CD1d1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.500.10">
        <property type="entry name" value="MHC class I-like antigen recognition-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050208">
        <property type="entry name" value="MHC_class-I_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011161">
        <property type="entry name" value="MHC_I-like_Ag-recog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037055">
        <property type="entry name" value="MHC_I-like_Ag-recog_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011162">
        <property type="entry name" value="MHC_I/II-like_Ag-recog"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675">
        <property type="entry name" value="MHC CLASS I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16675:SF130">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16497">
        <property type="entry name" value="MHC_I_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54452">
        <property type="entry name" value="MHC antigen-recognition domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0967">Endosome</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0391">Immunity</keyword>
    <keyword evidence="14" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="13" id="KW-0458">Lysosome</keyword>
    <keyword evidence="10 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="18" id="KW-0732">Signal</keyword>
    <keyword evidence="5 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040134905" description="Ig-like domain-containing protein" evidence="18">
        <location>
            <begin position="19"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="298"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="19">
        <location>
            <begin position="185"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00037203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00038793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005004544.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="330" mass="36568" checksum="F3DBC15290B05567" modified="2023-09-13" version="2">MLLLPLLLSAVLFPGGDNEHAFQGPTSFHLIQISSFANSTWAQNQGSGWLDDLQIHGWDSDSGTAIFLKPWSKGNLSDEEVTELEELFRVYLIGFIREVQDRVSEFQMEYPFEIQGIAGCELHSGGTTVSFLRGALGGLDFLSIKNESCAPAPEGGSRAQKFCTLISQYQGIADIIRKLLLETCPRYLLGVLDAGKAELQRQGNEARGLAVQWPGHLLLVCHVSGFYPKPVWVMWMRGEQEQPGTQQGDVLPNADGTWYLRVTLDVAAGEAAGLACRVRHSSLGGQDIILYWGRSTSIGLIVLAIIVPSLILLICFTLWFWRRWSYLNIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L913</accession>
    <name>A0A8C4L913_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Copine-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Copine II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CPNE2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Calcium-dependent phospholipid-binding protein that plays a role in calcium-mediated intracellular processes. Exhibits calcium-dependent cell membrane binding properties.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the copine family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014682824.1">
        <property type="nucleotide sequence ID" value="XM_014827338.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L913"/>
    <dbReference type="Ensembl" id="ENSEAST00005007952.2">
        <property type="protein sequence ID" value="ENSEASP00005007289.2"/>
        <property type="gene ID" value="ENSEASG00005005223.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822236"/>
    <dbReference type="KEGG" id="eai:106822236"/>
    <dbReference type="CTD" id="221184"/>
    <dbReference type="GeneTree" id="ENSGT00940000157653"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005544">
        <property type="term" value="F:calcium-dependent phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04047">
        <property type="entry name" value="C2B_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000103">
        <property type="entry name" value="Copine 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000042">
        <property type="entry name" value="Copine 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037768">
        <property type="entry name" value="C2B_Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045052">
        <property type="entry name" value="Copine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010734">
        <property type="entry name" value="Copine_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857">
        <property type="entry name" value="COPINE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10857:SF3">
        <property type="entry name" value="COPINE-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07002">
        <property type="entry name" value="Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="15">
        <location>
            <begin position="6"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="15">
        <location>
            <begin position="138"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007289.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="548" mass="60880" checksum="9C4DA60F912AFFF5" modified="2023-09-13" version="2">MAYTPGGGAPARGAAPVGSQYCVCKVELSVSGQNLLDRDITSKSDPFCVLFTENNGRWIEYDRTETAVNNLNPAFSKKFVLDYHFEEVQKLQFALFDQDKSSAQLDEHDFLGQFSCSLGTIVSSKKITRPLLLMNGKPAGKGLITIAAQELSDNRVVTLSLAGRKLDKKDLFGKSDPFLEFYKPGDDGKWMLVHRTEVIKYTLDPVWKPFTVPLVSLCDGDMEKPIQVMCYDYDNDGGHDFIGEFQTSVSQMCAAQDGIPLEFECINPKKQKKKKNYKNSGIIILRSCKISRDYSFLDYILGGCQLMFTVGIDFTASNGNPLDPSSLHYINPMGTNEYLSAIWAVGQIIQDYDSDKMFPALGFGAQLPPDWKVSHEFAINFNPTNPFCSGVDGIAQAYSACLPHIRFYGPTNFSPIVNHVARFAAQATQQQTATQYFILLIITDGVISDMEETRHAVVQASKLPMSIIIVGVGNADFAAMEFLDGDSRTLRSYTGEEAARDIVQFVPFREFRNAAKETLAKAVLAELPQQVVQYFKHKNLPPTHSEPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L9N3</accession>
    <name>A0A8C4L9N3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Importin 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">IPO7</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the importin beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L9N3"/>
    <dbReference type="Ensembl" id="ENSEAST00005005205.2">
        <property type="protein sequence ID" value="ENSEASP00005004751.2"/>
        <property type="gene ID" value="ENSEASG00005003575.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154666"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046332">
        <property type="term" value="F:SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045668">
        <property type="term" value="P:negative regulation of osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901331">
        <property type="term" value="P:positive regulation of odontoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900182">
        <property type="term" value="P:positive regulation of protein localization to nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000053">
        <property type="entry name" value="Importin 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:002058">
        <property type="entry name" value="Ipo7 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058669">
        <property type="entry name" value="TPR_IPO7/11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF27">
        <property type="entry name" value="IMPORTIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25758">
        <property type="entry name" value="TPR_IPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="9">
        <location>
            <begin position="22"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="836"/>
            <end position="865"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="839"/>
            <end position="865"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005004751.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="993" mass="114137" checksum="C77091F2CFE7343E" modified="2023-09-13" version="2">MDPNTIIEALRGTMDPALREAAERQLNEAHKSLNFVSTLLQITMSEQLDLPVRQAGVIYLKNMITQYWPDRETAPGDISPYTIPEEDRHCIRENIVEAIIHSPELIRVQLTTCIHHIIKHDYPSRWTAIVDKIGFYLQSDNSACWLGILLCLYQLVKNYEYKKPEERNPLVAAMQHFLPVLKDRFIQLLSDQSDQSVLIQKQIFKIFYALVQYTLPLELINQQNLTEWIEILKTVVNRDVPNETLQVEEDDRPELPWWKCKKWALHILARLFERYGSPGNVSKEYNEFAEVFLKAFAVGVQQGIIQDVIFPLMCYTDADEELWQEDPYEYIRMKFDVFEDFISPTTAAQTLLFTACSKRKEVLQKTMGFCYQILTEPNADPRKKDGALHMIGSLAEILLKKKIYKDQMEYMLQNHVFPLFSSELGYMRARACWVLHYFCEVKFKSDQNLQTALELTRRCLIDDREMPVKVEAAIALQVLISNQEKAKEYITPFIRPVMQALLHIIRETENDDLTNVIQKMICEYSEEVTPIAVEMTQHLAMTFNQVIQTGPDEEGSDDKAVTAMGILNTIDTLLSVVEDHKEITQQLEGICLQVIGTVLQQHVLEFYEEIFSLAHSLTCQQVSPQMWQLLPLVFEVFQQDGFDYFTDMMPLLHNYVTVDTDTLLSDTKYLEMIYSMCKKVLTGVAGEDAECHAAKLLEVIILQCKGRGIDQCIPLFVEAALERLTREVKTSELRTMCLQVAIAALYYNPHLLLNTLENLRFPNNVEPVTNHFITQWLNDVDCFLGLHDRKMCVLGLCALIDMEQIPQVLNQVSGQILPAFILLFNGLKRAYACHAEHENDSDDDDEAEDDDETEELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQTIQSRNPVWYQALTHGLNEEQRKQLQDIATLADQRRAAHESKMIEKHGGYKFSAPVVPSSFNFGGPAPGMN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LAT2</accession>
    <name>A0A8C4LAT2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Elongin-B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Elongin 18 kDa subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">RNA polymerase II transcription factor SIII subunit B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">SIII p18</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Transcription elongation factor B polypeptide 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ELOB</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Heterotrimer of an A (ELOA, ELOA2 or ELOA3P), ELOB and ELOC subunit. The elongin BC complex interacts with EPOP; leading to recruit the elongin BC complex to Polycomb group (PcG) target genes, thereby restricting excessive activity of the PRC2/EED-EZH2 complex. Component of multiple cullin-RING E3 ubiquitin-protein ligase complexes composed of Elongin BC (ELOB and ELOC), a cullin (either CUL2 or CUL5), a catalytic subunit (either RBX1 or RNF7/RBX2), as well as a substrate adapter protein that can be either ASB2, ASB9, ASB11, KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B, FEM1C, LRR1, PCMTD1, SOCS1, SOCS2, SOCS5, SPSB1, SPSB3, ELOA, VHL, WSB1 or RAB40C. As part of the Elongin BC E3 ubiquitin ligase complex; interacts with NRBP1. May also interact with DCUN1D1, DCUN1D2, DCUN1D3 and DCUN1D5. May form oligomers as a KLHDC2/KLHDC3-ELOB-ELOC complex; this interaction is autoinhibitory for the E3 ligase complex as the substrate-binding site of KLHDC2/KLHDC3 is blocked in the oligomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the Elongin B family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LAT2"/>
    <dbReference type="Ensembl" id="ENSEAST00005009099.2">
        <property type="protein sequence ID" value="ENSEASP00005008350.2"/>
        <property type="gene ID" value="ENSEASG00005006017.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018316"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070449">
        <property type="term" value="C:elongin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030891">
        <property type="term" value="C:VCB complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01788">
        <property type="entry name" value="Ubl_ElonginB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000108">
        <property type="entry name" value="Elongin-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039049">
        <property type="entry name" value="ELOB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13248:SF4">
        <property type="entry name" value="ELONGIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13248">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR B POLYPEPTIDE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="18">
        <location>
            <begin position="86"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="176"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="192"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00060803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00083653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00093515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008350.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="203" mass="22292" checksum="EC006617D3B09362" modified="2023-09-13" version="2">MSWPPGGPQPSETTEPGAEGLALLPTHILPHLIPTAGRVTALNGMEACSHLLQQTHQVDSTMGAQRPTLRRLRRNSSAWAKDSPLQDVFLMIRRHKTTIFTDAKESSTVFELKRIVEGILKRPPDEQRLYKDDQLLDDGKTLGECGFTSQTARPQAPATVGLAFRADEAFEALRIEPFSSPPELPDVMKPQDSGSSANEQAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LAU9</accession>
    <name>A0A8C4LAU9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">CASP8-associated protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">FLICE-associated huge protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CASP8AP2</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">PML body</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014722180.2">
        <property type="nucleotide sequence ID" value="XM_014866694.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070352379.1">
        <property type="nucleotide sequence ID" value="XM_070496278.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005009123.2">
        <property type="protein sequence ID" value="ENSEASP00005008374.2"/>
        <property type="gene ID" value="ENSEASG00005006023.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106847418"/>
    <dbReference type="KEGG" id="eai:106847418"/>
    <dbReference type="CTD" id="9994"/>
    <dbReference type="GeneTree" id="ENSGT00620000088063"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016605">
        <property type="term" value="C:PML body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008625">
        <property type="term" value="P:extrinsic apoptotic signaling pathway via death domain receptors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036337">
        <property type="term" value="P:Fas signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12202">
        <property type="entry name" value="CASP8AP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000265">
        <property type="entry name" value="CASP8-associated protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039674">
        <property type="entry name" value="FLASH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049257">
        <property type="entry name" value="Gon4l/CASP8AP2_myb-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15489:SF2">
        <property type="entry name" value="CASP8-ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15489">
        <property type="entry name" value="CASPASE 8 ASSOCIATED PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21227">
        <property type="entry name" value="Myb_DNA-binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="144"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="229"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="568"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="868"/>
            <end position="1011"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1794"/>
            <end position="1905"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="73"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="157"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="229"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="286"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="407"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="446"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="464"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="574"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="887"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="897"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="923"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="933"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="993"/>
            <end position="1009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1845"/>
            <end position="1859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="1869"/>
            <end position="1878"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00069865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008374.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1975" mass="221495" checksum="B101985DFD855336" modified="2023-09-13" version="2">MAADDDNGDGTSLFDVFSASPLKNNDEGSLDIYAGLDSAVSDNASKSCVPSRNCLDLYEEILTEEGTAKEATYNDLQVEYGKCQMQMKELMKKFKEIQTQNFSLKNENQSLKKNISALIKTARVEINRKDEEINNLHQRLSEFPHFRNNHKTSRTSDIVKTKDLKSRSPHLDDCSKTDHRVKSDVSKDVHHSTSLPNLEKEGKSHSGKKGTLHLPTSLEKHCTNGIWSRSHYQVGEGSSNEDSRRGRKDTRHSQYSRGTDRIRKDLNTNCGDGEPRNTEASQRLQGRPEKYGKGEPKAESKNSKFKSNTDLDYKNERLSSSWEKETSRERSHTRTESQSDKKPERQSERSQNINRKEFKSQDKEERKVDQKPKSIVKDQDYWRRSKRASFPHSKNEITKSSHNSGKYHVEERRGREDCKRDRGVSNHSFQEGRCPSSFSTSRTHKHIDSKEVDAVHQWENTPLKAERHRTEDKRKRERESKDENRHMRNEKRTPTEHLQKTNKETKKTTTDLKRQHEPKNDKGEVSNNDISQEGDNKELTSKAESGPNETKNKDLKLSFMEKLNLTLSPAKKQPVSQDNQHKITNTPKSSGICDVESLVQAKTVTCVPSVSEHITEETKSKVLEPNDALAAESESRTKIPERKMEEENSLLVKSVENTMPCDVPVCGTETSFSAPVEMEKAESLFPSSTEMEQTINGAGAAVPVVMDILQTNVSQNFGLELDIKRNDGLNSCSISEGIEMKEAFSTQVAESSGSILQPSIEEDGVLPIVLSEDGNPQFEPSLVGTPLVENKSCHLEPCSPKEPLESSLQQTELMDHRVEIGETNSIYHDDENSVLSIDLNHLRPIPEAISPLNSPVRPVAKVLRMESPSQGPLYSNSHKDVFPPDSAHSTSKSQSDLNKENQKPICKSDRFTEADSLKNSSLDELEEGEIISDSEKSKPQKSFEKSAKPRASADMQHTKTSPGSRKTAVHLDKDSRKTSSVKIHQTKSKWNKRQSESSRSSKTEKKDKTVSTSSLEKIVPIIAPPFSVREVMHMLRMIRKHVRKNYMKFKVKFSLLQFHRIVESAILSFTSLIKRLDLSKISKSVTVLQKNLCDVIESKLKQVKKNGIVDRLFEQQLPDMKKKLWKFVDEQLDYLFAKLKKILVKFCDSVNFGSDSDEGKLEKRNKEKSQYLNCRKGNVDSSSKEILKEKSPTSEGSVYYRSLVGCKKSEEKHQDQNCSNINTVKYDIKKSFNSCFDNIKNSQSEEHSLELNCLSAPKPGKIEGSTVEDAQTSQHAALKPERSFEILTEQQASSLTFNLVSDAQMGEIFKSLLQGSDLLDTSVNCNEKSEWELKTPEKQLLDSLKCESIPACTTEELVSGVVSPCPKMISDDNWSLLSSEKAPSLSSGLSLPVHPDVLDESCMFEVSTNIALSKDNVCSSEKSKPCISSILLEDLAVSLTVPSPLKSDGHLSFLKPEVLSTSTPEEVISAHFSEDALLEEEDASEQDIHLALESDNSSSKSSCSSSWTSRSVVPGFQYHPNLPMHAVIMEKSNDHFIVKIRRAAPSTSPNLKQNMVADESLASLPRVEKEADEAAEKEYTSCQNRVFKPVEELQNSNKHVDSSKSAQEEEDCVIQTQVPDIYEFLKDASGKVGHSNEVADECFKLHHEWEPKVPESVEELPPVEEIPHSVEDHLPNTYIDLTKDPVTETKNLGEFIEVTVLNIDQFGCSGGNLDQNAPILDNMQPDTVGAFIDLTQDVSSESKNEGNCPALAVEGLGCQVICVDEDNCKEEKVQVANRPLECVVEEAYIDLTSESPSSCEVKQDDVKSEPAPNSHSSEVPGALDNAQKKRKNLSDLNHSQKKQRKETDLTSREKTKKITQDSGENGEAHRKKANKKKAPAVTQDSSLKASPGIKEPSAASATSTGLSAKNVIKKKGEIIVSWTRNDDREILLECQKRGPSLKTFSYLAAKLNKNPYQVSERFQQLMKLFEKSKCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LD56</accession>
    <name>A0A8C4LD56_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LD56"/>
    <dbReference type="Ensembl" id="ENSEAST00005008652.2">
        <property type="protein sequence ID" value="ENSEASP00005007933.2"/>
        <property type="gene ID" value="ENSEASG00005005762.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160874"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF550">
        <property type="entry name" value="TETRASPANIN-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="16"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007933.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="26008" checksum="A4D5854F81ABCCC2" modified="2023-09-13" version="2">MCLCWWRMKEGERSPLLSQDAGGQEPPLSGSSPLTSSHLGPALQEGQAWRTGLGGGLVPFLPSGRSCLKCLTFLSNFLFSLLSLLALAMGLWGLAIRGSLGNSWGGPLPADPMLGLVLGGLAVSVVSLAGCLGALCENACLLRCFSGGLLTFLVLEAVWGALVVALWGPLQDGLEHALRVAITHYQDDPDLRFLMDQVQLRLQCCGAASYQDWQRNLMACQVVPCPHLGSKPANPRPPKWNMGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LDN3</accession>
    <name>A0A8C4LDN3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">YME1L1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LDN3"/>
    <dbReference type="Ensembl" id="ENSEAST00005010369.2">
        <property type="protein sequence ID" value="ENSEASP00005009547.2"/>
        <property type="gene ID" value="ENSEASG00005006732.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074836"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000195">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FTSH 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
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    <dbReference type="HAMAP" id="MF_01458">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF97">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE YME1L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="615"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="17">
        <location>
            <begin position="313"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009547.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
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    <accession>A0A8C4LEL6</accession>
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            <fullName evidence="6">Zinc finger BED-type containing 5</fullName>
        </submittedName>
    </protein>
    <gene>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_014844176.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014699663.1">
        <property type="nucleotide sequence ID" value="XM_014844177.3"/>
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        <property type="nucleotide sequence ID" value="XM_044752351.2"/>
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        <property type="nucleotide sequence ID" value="XM_070491098.1"/>
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        <property type="nucleotide sequence ID" value="XM_070491101.1"/>
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        <property type="gene ID" value="ENSEASG00005005885.2"/>
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    <dbReference type="Ensembl" id="ENSEAST00005081930.1">
        <property type="protein sequence ID" value="ENSEASP00005062893.1"/>
        <property type="gene ID" value="ENSEASG00005005885.2"/>
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        <property type="component" value="Chromosome 20"/>
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    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003656">
        <property type="entry name" value="Znf_BED"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913">
        <property type="entry name" value="EPM2A-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913:SF19">
        <property type="entry name" value="LOW QUALITY PROTEIN: ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02892">
        <property type="entry name" value="zf-BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50808">
        <property type="entry name" value="ZF_BED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="BED-type" evidence="5">
        <location>
            <begin position="108"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="693" mass="79045" checksum="A965A61D9F72A68C" modified="2022-01-19" version="1">MIAHLLCILSYNFNTFVILNVYSKLTMFCTTNTLPMDLLLKQGSLKQEVESFCYQIVSESNDQKVGILQDEDEQLQPSVSKKSEGELSRVKFMSSSNKITFSKKPKRRKYDESYLSFGFTYFGNRDAPHAQCVLCKKILSNSSLAPSKLRRHLETKHAAYKDKDISFFKQHLDSPENNKPPTPKIVNTDNESATEASYNVSYHIALSGEAHTIGELLIKPCAKDVVMRMFDEQYSKKIDAVQLSNSTVARRIKDLAADIEEELVCRLKICDGFSLQLDESADVSGLAVLLVFVRYRFNKSIEEDLLLCESLQSNATGEEIFNCINGFMQRHEIEWEKCVDVCSDASRAMDGKIAEAVTLIKYVAPESTSSHCLLYRHALAVKIMPTTLKNVLDQAVQIINYIKARPHQSRLLKILCEEMGAQHTALLLNTEVRWLSRGKVLVRLFELRRELLVFMDSAFRLSDCLTNSSWLLKLAYLADIFTKLNEVNLSMQGKNVTVFTVFDKMSSLLRKLEFWASSVEEENFDCFPTLSDFLTEINSTVDKDICSAIVQHLRGLRSTLLKYFPVTNDNNAWVRNPFTVTVKPASLVARDYESLIDLTSDSQVKQNFSELSLNDFWSSLIQEYPSIARRAVRVLLPFATMHLCETGFSYYAATKTKYRKRLDAAPHMRIRLSNITPNIKRICDKKTQKHCSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LFR9</accession>
    <name>A0A8C4LFR9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Rab3 GTPase-activating protein catalytic subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RAB3GAP1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">cis-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Rab3-GAP catalytic subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LFR9"/>
    <dbReference type="Ensembl" id="ENSEAST00005011381.2">
        <property type="protein sequence ID" value="ENSEASP00005010457.2"/>
        <property type="gene ID" value="ENSEASG00005007419.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006705"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045700">
        <property type="entry name" value="Rab3GAP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045698">
        <property type="entry name" value="Rab3GAP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026147">
        <property type="entry name" value="Rab3GAP1_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21422">
        <property type="entry name" value="RAB3 GTPASE-ACTIVATING PROTEIN CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21422:SF9">
        <property type="entry name" value="RAB3 GTPASE-ACTIVATING PROTEIN CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19533">
        <property type="entry name" value="Rab3-GAP_cat_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13890">
        <property type="entry name" value="Rab3-GTPase_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab3GAP catalytic subunit conserved" evidence="11">
        <location>
            <begin position="615"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="domain" description="Rab3GAP catalytic subunit C-terminal" evidence="12">
        <location>
            <begin position="781"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="533"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="910"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="561"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="920"/>
            <end position="933"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF13890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF19533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005010457.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="989" mass="111429" checksum="668FCBB1B909DF65" modified="2023-09-13" version="2">MAADSEPESEVFEITDFTTASEWERFISKVEEVLNDWKLIGNSLGKPLEKGVFTSGTWEEKSDEISFADFKFSVTHHYLVQESTDKEGKDEVLEDVIPQAMQDLLCMNNDFPPRAHCLVRWYGLREFVVIAPAANSDAVLSESKCNLLLSSVSIALGNTGCQVPLFVQIHHKWRRMYVGECQGPGVRTDFEMVHLRKVPNQYTHLSGLLDIFKSKIGCPLTPLPPVSIAIRFTYVLQDWQQYFWPQQPPDIDALVGGEVGGLEFGKLPFGACEDPISELHLATTWPHLTEGIIVDNDVYSDLDPIQAPHWSVRVRKADNPQCLLGDFVTEFFKICRRKESTDEILGRSTFEEEGREIADITHALSKLTEPAPVPIHKLSVSNMVHTAKKKIRKHRGVEESPLNNDVLNTILLFLFPDAASEKPLDGTSSTDNNNPPSESEEYNLYNQFKSAPSDSLTYKLALCLCMINFYHGGLKGVAHLWQEFVLEMRFRWENNFLIPGLASGPPDLRCCLLHQKLQMLNCCIERKKARDEGRKTNTSDNVTNTYPGDAGKAGDQLGPDNLKDMDKEKGEMGKSWDSWSDSEEEFFECLSDTEELKGNGQEGGKKGGPKEMANLKPEGRLIQHGKLTLLHNGEPLYIPVTQEPAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCFLEDFVRWYSPRDYIEEETIDEKGNMVLKGELSARMKIPSNMWVEAWETAKPIPARRQRRLFDDTREAEKVLHYLAVQKPADLARHLLPCVIHAAVLKVKEEESLENISSVKKIIKQIISHSSKVLHFPNPEDKKLEEIIHQITNVEAIIARARSLKAKFGTERCEQEEEKEDLERFVSCLLEQPEVLVVGAGRGHAGRIIHKLFVNAQRLTESSDEAAAMAPAEEELKRTGSPEERRQNSVSDFPPPAGRELILRSTVPRPAPYSRALPQRMYSVLTREDFRLAGAFSSDTSFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LKD2</accession>
    <name>A0A8C4LKD2_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Tensin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TNS2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Focal adhesion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PTEN phosphatase protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044611460.1">
        <property type="nucleotide sequence ID" value="XM_044755525.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005014458.2">
        <property type="protein sequence ID" value="ENSEASP00005013312.2"/>
        <property type="gene ID" value="ENSEASG00005009273.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835272"/>
    <dbReference type="CTD" id="23371"/>
    <dbReference type="GeneTree" id="ENSGT00940000161535"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20887">
        <property type="entry name" value="C1_TNS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01213">
        <property type="entry name" value="PTB_tensin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09927">
        <property type="entry name" value="SH2_Tensin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000039">
        <property type="entry name" value="Tensin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000002">
        <property type="entry name" value="Tensin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000010">
        <property type="entry name" value="tensin-1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013625">
        <property type="entry name" value="PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006020">
        <property type="entry name" value="PTB/PI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035012">
        <property type="entry name" value="Tensin-like_SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014020">
        <property type="entry name" value="Tensin_C2-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029023">
        <property type="entry name" value="Tensin_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033929">
        <property type="entry name" value="Tensin_PTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051484">
        <property type="entry name" value="Tensin_PTEN_phosphatase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45734">
        <property type="entry name" value="TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45734:SF1">
        <property type="entry name" value="TENSIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08416">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10409">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01326">
        <property type="entry name" value="PTEN_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51182">
        <property type="entry name" value="C2_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51181">
        <property type="entry name" value="PPASE_TENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50001">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 10" id="KW-0727">SH2 domain</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="13">
        <location>
            <begin position="31"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatase tensin-type" evidence="14">
        <location>
            <begin position="122"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="C2 tensin-type" evidence="15">
        <location>
            <begin position="299"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="SH2" evidence="12">
        <location>
            <begin position="1141"/>
            <end position="1248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="462"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="813"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="492"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="514"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="546"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="858"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="899"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="930"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1046"/>
            <end position="1056"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005013312.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1410" mass="152560" checksum="1C9A96044CEA2924" modified="2023-09-13" version="2">MGSPQTMEEGVQPSVPRWAALCIVKPRKAEPHSFREKVFRKKPPVCAVCKVTIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAAAFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMEMWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYINYFSGLLSGSIRMNSSPLFLHYVLVPMLPAFEPGTGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKSGRGTDRTLVFRVQFHTCTIHGPRLTFTKDQLDEAWTDERVPFQAAVEFVFSSSPENIKGHTPRNDPSVTVDYNTAEPAVRWDSYENFNQHHEDSVDGSLTHTQGPLDGSPYAQVQRGPRQTPPAPSPEPPPPPMLSVSSDSGHSSTLTTEPTAESPGRPPPTAAERQELDRLLGGCGVASGGRGAGRETAILDDEEQPPTAGGPPLGMYSGHRPGLSRHCSCRQGYREGCGVPNGGYYRPEGTLERRRLAYGGYEGHPQGYTEPSVEKRRLCRSLSEGPHPYLPELGKPANGDCGYRAAGYREVVILEDPGLPALCSCPTCEEKLALPTAALYGLRLEREAGEGWVSEASKPLLHPMRPGHPLPLLVPACGHHHAPVPDYSCLKPPKAGEEGHEGCPCAGCPEGRYGHPGYPALVTYSYGGAVPSYCPAYGRVPHSCGAPGEGRGYPSPGAHSPRAGSISPGGPPSPQSRKLNYEIPAEEGGDRYPLPGHLAPAGPLTSAESPEPVSWREGPSGHSTLPRSPRDAQCSASSELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPGEALPPVSQGGAEKAPELPARSGLEPPTPSPFSSTSPPSSPSDWPQERSPGGRSDSASPRGHVPTTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDISGPTQPPLPEKRHLPGPGQQPGGPWGPEQASPPARGTSHHVTFAPLLPDNVPQPPEPPVQESQSNVKFVQDTSKFWYKPHLSRDQAIALLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQPWKGDPLEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEETPEAPVPTNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNPDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LKQ7</accession>
    <name>A0A8C4LKQ7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Lysosomal-associated transmembrane protein 4A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LAPTM4A</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">May function in the transport of nucleosides and/or nucleoside derivatives between the cytosol and the lumen of an intracellular membrane-bound compartment.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LAPTM4/LAPTM5 transporter family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014703526.1">
        <property type="nucleotide sequence ID" value="XM_014848040.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LKQ7"/>
    <dbReference type="Ensembl" id="ENSEAST00005014641.2">
        <property type="protein sequence ID" value="ENSEASP00005013477.1"/>
        <property type="gene ID" value="ENSEASG00005009414.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835581"/>
    <dbReference type="KEGG" id="eai:106835581"/>
    <dbReference type="CTD" id="9741"/>
    <dbReference type="GeneTree" id="ENSGT00940000153446"/>
    <dbReference type="OMA" id="NCYKYII"/>
    <dbReference type="OrthoDB" id="10002163at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004687">
        <property type="entry name" value="LAPTM4/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018396">
        <property type="entry name" value="LAPTM_4A/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051115">
        <property type="entry name" value="LAPTM_transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00799">
        <property type="entry name" value="mtp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12479">
        <property type="entry name" value="LYSOSOMAL-ASSOCIATED TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12479:SF5">
        <property type="entry name" value="LYSOSOMAL-ASSOCIATED TRANSMEMBRANE PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03821">
        <property type="entry name" value="Mtp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="108"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="154"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005013477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="233" mass="26891" checksum="121B6BEEB892A988" modified="2022-01-19" version="1">MVSMTFKRSRSDRFYSNRCCGCCHVRTETIILGTWYMVVNLLMAILLTVEVTHPNSMPAVNIQYEVIGNYYSSERMADNACVLFAVSVLMFIISSMLVYGAISYQVGWLIPFFCYRLFDFVLSCLVAISSLTYLPRIKEYLDQLPDFPYKDDLLALDSSSLLFIVLTFFALFIIFKAYLINCVWNCYKYINNRNMPEIAVYPAFEAPPQYVLPTYEMAVKMPEKEPPPPYIPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LQV5</accession>
    <name>A0A8C4LQV5_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tropomodulin 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">TMOD3</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tropomodulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014706003.1">
        <property type="nucleotide sequence ID" value="XM_014850517.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LQV5"/>
    <dbReference type="SMR" id="A0A8C4LQV5"/>
    <dbReference type="Ensembl" id="ENSEAST00005015614.2">
        <property type="protein sequence ID" value="ENSEASP00005014371.2"/>
        <property type="gene ID" value="ENSEASG00005010006.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837142"/>
    <dbReference type="KEGG" id="eai:106837142"/>
    <dbReference type="CTD" id="29766"/>
    <dbReference type="GeneTree" id="ENSGT00940000158280"/>
    <dbReference type="OrthoDB" id="2163268at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005865">
        <property type="term" value="C:striated muscle thin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005523">
        <property type="term" value="F:tropomyosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048821">
        <property type="term" value="P:erythrocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044772">
        <property type="term" value="P:mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006936">
        <property type="term" value="P:muscle contraction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030239">
        <property type="term" value="P:myofibril assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051694">
        <property type="term" value="P:pointed-end actin filament capping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901992">
        <property type="term" value="P:positive regulation of mitotic cell cycle phase transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000006">
        <property type="entry name" value="Tropomodulin 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004934">
        <property type="entry name" value="TMOD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901">
        <property type="entry name" value="TROPOMODULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10901:SF18">
        <property type="entry name" value="TROPOMODULIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03250">
        <property type="entry name" value="Tropomodulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014371.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="352" mass="39659" checksum="E7AED1A47C9EBEDA" modified="2023-09-13" version="2">MALPFRKDLEKYKDLDEDELLGNLSEMELKQLETVLDDLDPENALLPAGFRQKNQTSKSATGPFDREHLLSYLEKEALEHKDREDYVPYTGEKKGKIFIPKQKPIQTFTEEKVSLDPELEEALTSASDTELCDLAAILGMHNLITNTQFCTIVGSSNGVDQEHFSNVVKGETILPVFDEPPNPTNVEESLKRIKENDVRLVEVNLNNIKNIPIPTLKDFAKALETNTHVKHFSLAATRSNDPVAVAFADMLRVNKNLKSLNMESNFITGAGILALIDALRDNETLAELKIDNQRQQLGTAVELEMAKMLEENTNILKFGYQFTQQGPRTRAANAITKNNDLVRKRRVEGDHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LRJ0</accession>
    <name>A0A8C4LRJ0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DBH-like monooxygenase protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Monooxygenase X</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">MOXD1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the copper type II ascorbate-dependent monooxygenase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LRJ0"/>
    <dbReference type="Ensembl" id="ENSEAST00005017322.2">
        <property type="protein sequence ID" value="ENSEASP00005015934.2"/>
        <property type="gene ID" value="ENSEASG00005011072.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063085"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030667">
        <property type="term" value="C:secretory granule membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004500">
        <property type="term" value="F:dopamine beta-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042420">
        <property type="term" value="P:dopamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042421">
        <property type="term" value="P:norepinephrine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006589">
        <property type="term" value="P:octopamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09631">
        <property type="entry name" value="DOMON_DOH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.310:FF:000002">
        <property type="entry name" value="DBH-like monooxygenase protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.230:FF:000001">
        <property type="entry name" value="Monooxygenase, DBH-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.310">
        <property type="entry name" value="Copper type II, ascorbate-dependent monooxygenase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014784">
        <property type="entry name" value="Cu2_ascorb_mOase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000323">
        <property type="entry name" value="Cu2_ascorb_mOase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036939">
        <property type="entry name" value="Cu2_ascorb_mOase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024548">
        <property type="entry name" value="Cu2_monoox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000945">
        <property type="entry name" value="DBH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045266">
        <property type="entry name" value="DOH_DOMON"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005018">
        <property type="entry name" value="DOMON_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008977">
        <property type="entry name" value="PHM/PNGase_F_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028460">
        <property type="entry name" value="Tbh/DBH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10157:SF28">
        <property type="entry name" value="DBH-LIKE MONOOXYGENASE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10157">
        <property type="entry name" value="DOPAMINE BETA HYDROXYLASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03712">
        <property type="entry name" value="Cu2_monoox_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01082">
        <property type="entry name" value="Cu2_monooxygen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03351">
        <property type="entry name" value="DOMON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00767">
        <property type="entry name" value="DBMONOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00664">
        <property type="entry name" value="DoH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49742">
        <property type="entry name" value="PHM/PNGase F"/>
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    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0503">Monooxygenase</keyword>
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            <begin position="1"/>
            <end position="18"/>
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        <location>
            <begin position="19"/>
            <end position="615"/>
        </location>
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        <location>
            <begin position="35"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010676"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00080850"/>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005015934.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="615" mass="69562" checksum="706D1FBA85FA9903" modified="2023-09-13" version="2">MRAGPLLLLGALLGGAAAGGSGRPLPHRTLLDPDGGYWLSWGPRGGRLAFRLEVRTAGYVGFGFSPTGAMAAADIVVGGVAHGRPYLQDYFTNANRELKKDTQQDYHLEYAMENSTHTVIEFTRELHTCDVNDRTLTESTVRVIWAYHHEDVGEGGPKYHGSNRGTRSLRLLNPEKTNVLSAATPYFDLVNQDVPIPTKGTTYWCQMFKIPVFQEKHHVIKVEPVIQRGHESLVHHILLYQCSSSFNDSVLDYGHECYHPNMPDAFLTCETVMFAWAIGGEGFSFPPHVGLSLGTPLDPHYVLLEVHYDNPTYKEGLIDNSGLRIFHTTEIRKYDAGAIEAGLWVSLFHTIPPGMPEFRSEGHCTLECLQEALEAEKPSGIHVFAVLLHAHLAGRGIRLRHFRKGEEMRLLAYDDDFDFNFQEFQYLKEEQTILPGDNLITECRYNTKDRGRMTWGGLSTRNEMCLSYLLYYPRINLTRCASIPDIMEQLQFIGVKEIYRPVTTWPFIIKSPKQYRNLSFMDAMNKFKWTKKEGLSFNKLVLSLPVNVRCSKTDNAEWSIQGMTALPPDIERPYKAEPLVCGTSRSSSLRRNVSLKLLVGLTALGSMPSSWHWWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8C4LTV4_EQUAS</name>
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        <submittedName>
            <fullName evidence="8">Transmembrane protein 54</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TMEM54</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM54 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LTV4"/>
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        <property type="protein sequence ID" value="ENSEASP00005016987.2"/>
        <property type="gene ID" value="ENSEASG00005011772.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020977">
        <property type="entry name" value="Beta-casein-like"/>
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    <dbReference type="PANTHER" id="PTHR31258">
        <property type="entry name" value="KERATINOCYTE-ASSOCIATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31258:SF2">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12304">
        <property type="entry name" value="BCLP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="92"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="144"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="183"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005016987.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="208" mass="22367" checksum="5AA15E96941D4C3A" modified="2023-09-13" version="2">MCLRFGVMSVDDFRKVLMKTGLVLVVLGHVSFIAAALLHGTVLRYVAAPRDAVALQYCVVNILSVTSAIVVITSGIVAIVLSRYLPSTPLRWTVFSSSVACALLSLTCGRALLAACTFGSPEVLALAPDCPYDPTRIYSSSLCLWGISLVFCVAESVFAVRCAQLAHQLLKLRPWWGESSHHMVRPESPGPMEGQDLLSCTASEPLTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LY30</accession>
    <name>A0A8C4LY30_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">RUN and FYVE domain-containing protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">RUFY4</name>
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        <name type="scientific">Equus asinus</name>
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    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">ARL8 effector that promotes the coupling of endolysosomes to dynein-dynactin for retrograde transport along microtubules. Acts by binding both GTP-bound ARL8 and dynein-dynactin. In nonneuronal cells, promotes concentration of endolysosomes in the juxtanuclear area. In hippocampal neurons, drives retrograde transport of endolysosomes from the axon to the soma. Positive regulator of macroautophagy in dendritic cells. Increases autophagic flux, probably by stimulating both autophagosome formation and facilitating tethering with lysosomes. Binds to phosphatidylinositol 3-phosphate (PtdIns3P) through its FYVE-type zinc finger. Positive regulator of osteosclast bone-resorbing activity, possibly by promoting late endosome-lysosome fusion by acting as an adapter protein between RAB7A on late endosomes and LAMP2 on primary lysosomes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LY30"/>
    <dbReference type="Ensembl" id="ENSEAST00005019647.2">
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        <property type="gene ID" value="ENSEASG00005012495.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 19"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0034452">
        <property type="term" value="F:dynactin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071353">
        <property type="term" value="P:cellular response to interleukin-4"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061763">
        <property type="term" value="P:multivesicular body-lysosome fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045780">
        <property type="term" value="P:positive regulation of bone resorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045921">
        <property type="term" value="P:positive regulation of exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001019">
        <property type="term" value="P:positive regulation of retrograde axon cargo transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.900:FF:000015">
        <property type="entry name" value="RUN and FYVE domain containing 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042939">
        <property type="entry name" value="RUFY4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059036">
        <property type="entry name" value="RUFY4_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47732">
        <property type="entry name" value="RUN AND FYVE DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47732:SF1">
        <property type="entry name" value="RUN AND FYVE DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25366">
        <property type="entry name" value="RUFY4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00593">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50826">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0072">Autophagy</keyword>
    <keyword evidence="7 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0458">Lysosome</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RUN" evidence="14">
        <location>
            <begin position="35"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="181"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="382"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="409"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="382"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00059075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00069100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005018097.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="572" mass="63532" checksum="FAEA9A501F153F32" modified="2023-09-13" version="2">MLMAEEGAVLKVTRDLKAAVSAILQGHGAGQQPVTDASAELHRLCGCLEHLLQFDQKEQRSFLRPRKGYWDFLCTALSQQRGDTEPASFVHSQDKLKTPLGKGRAFIRFCLAHGQLAETLQLCLLNPELTREWYGPRSPLVCPELREDILDSLYALNGVAFDLDLQRPDLDGAWPMFSESCCSNSSRAQGRRPRKTRDSPKEIATCEGPTGVQPEEPHASQASCVRDAPRGDPLARLSRSQQHKHCTPFLEKKREEPGSLGPPQSMREPEVEELLQDQDKRAPRMRICLENSTASIQGQGEGVQGALKEVTGAENEGRGVLLGAEGQRTIEGTPEGGAAWGHVQRLLGSSPRGTIEDAMSASGQEGEVASILGKPQVFQSLEAKEGSTAEKPQEQIGVTSVTRREEQAEAALQDVVESLRRGLQKTKEEARHQEQLLKEQEGELEALREKLSRCQEERARLRAELEQKQQEANRRDAAHEEELGGQRDLVRALKRRVLELTQEKDNLWQKVQHLSSLAPGCCVSCSKIFGRLSRRYPCRLCGGRICHACSVDYRKRGHRCPPCFQKGEAQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LYZ6</accession>
    <name>A0A8C4LYZ6_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">T-box transcription factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TBX1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LYZ6"/>
    <dbReference type="Ensembl" id="ENSEAST00005016346.2">
        <property type="protein sequence ID" value="ENSEASP00005015023.2"/>
        <property type="gene ID" value="ENSEASG00005010505.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154816"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20187">
        <property type="entry name" value="T-box_TBX1_10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.820:FF:000006">
        <property type="entry name" value="T-box transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018186">
        <property type="entry name" value="TF_T-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF104">
        <property type="entry name" value="T-BOX TRANSCRIPTION FACTOR TBX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00425">
        <property type="entry name" value="TBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01283">
        <property type="entry name" value="TBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01264">
        <property type="entry name" value="TBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="T-box" evidence="9">
        <location>
            <begin position="108"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="T-box" evidence="7">
        <location>
            <begin position="113"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="24"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="312"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="370"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="324"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005015023.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="484" mass="51671" checksum="70FF395D379F7F9B" modified="2023-09-13" version="2">MHFSTVTRDMEAFTASSLSSLGAAGGFPGAASPGADPYGPREPPPPRYDPCAAAAPGPPPPHAYPFAPAAGAATSAAAEPEGPGASCAAAGKAPVKKNAKVASVSVQLEMKALWDEFNQLGTEMIVTKAGRRMFPTFQVKLFGMDRADLLDFVPVDDKRYRYAFHSSSWLVAGKADPATPGRVHYHPDSPAKGAQWMKQIVSFDKLKLTNNLLDDNGHIILNSMHRYQPRFHVVYVDPRKDSEKYAEENFKTFVFEETRFTAVTAYQNHRITQLKIASNPFAKGFRDCDPEDWPRNHRPGALPLMSAFARSRNPVASPTPPNGAEKDAAEARREFERDAGGPAVLGDPAHPPQLLARVLSPALPGAGAGGLVPLPGAPGGRPSPPHSELRLEAPGASEPLHHHPYKYPAAAYDHYLGAKSRPAPYPLPGLRGHSYHPHAHPHHHHHHPAVSPAAAAAAAAAAAAANMYSSAGAAPPGSYDYCPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LZD0</accession>
    <name>A0A8C4LZD0_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Rhotekin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RTKN</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
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                <person name="Guo"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <source>
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    <sequence length="711" mass="79101" checksum="DA51BDCDC229FA7E" modified="2023-09-13" version="2">MKSREQLVQQVLQELQEAVESEGLEGLVGAALEAKQVLSSFTLPTCREGGPGPQVLEVDPVALSLYPEDAPRNMLPLMCKGEGSLLFEAVSMLLWGDAGLSLELRARTVVEMLLHRHYYLQGMIDSKVMLQAVRYSLCSEESPEMTSLPSATLEAIFDADVKATCFPSSFSNVWHLYALASVLQRNIYSIYPMRNLKIRPYFNRVIRPRRCDHTPATLHIMWAGQPLTSHLFRHQYFAPVVGLEEVEAEGTTGPALAPSALAPLPPPAKTLELLNREPGLSYSHLCERYSVTKSTFYRWRRQSQEHRQKVATRFSAKHFLQDSFHRGGLVPLQQFLQRFPEISRSTYYAWKHELLGSGACQALAPKEALAMEELEKLPEEQVVEGLGCSSLAVSSPGMVLMQRAKLYLEHCISLNTLVPYRCFKRRFPGISRSTYYNWRRKALRRNPTFKPPPALPVAGSPQPTSIGEEALLPWKGEVGEGAAKAMGEGPPAPREFLPLRMPLSRWQRRLRRAARKQVLSGHLPFCRFRLRYPSLSPSAFWVWKSLARGWPRSLSKLQVQASTLGKAGVQEAGEKQEKEAGRNMTAAMTPPAGTPKVGASPGEDPGKAEGGPSREGVLPGAATAQGRPHSGSLSSRPAVAVAGGRDGQVLVMDMLTTTKFKAQAKLFLQKRFQSKSFPSYKEFSALFPFTARSTYYMWKRALYDGLTLVDG</sequence>
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        <alternativeName>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Together with BOLA2, acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates [2Fe-2S] cluster insertion into a subset of cytosolic proteins. Acts as a critical negative regulator of cardiac hypertrophy and a positive inotropic regulator. Required for hemoglobin maturation. Does not possess any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer; the homodimer is independent of 2Fe-2S clusters. Heterotrimer; forms a heterotrimeric complex composed by two BOLA2 molecules and one GLRX3 molecule; linked by [2Fe-2S] clusters. Interacts (via N-terminus) with PRKCQ/PKC-theta. Interacts (via C-terminus) with CSRP3. Interacts with CSRP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Myofibril</location>
            <location evidence="1">Sarcomere</location>
            <location evidence="1">Z line</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044604482.1">
        <property type="nucleotide sequence ID" value="XM_044748547.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M5E7"/>
    <dbReference type="Ensembl" id="ENSEAST00005023742.2">
        <property type="protein sequence ID" value="ENSEASP00005021881.2"/>
        <property type="gene ID" value="ENSEASG00005014940.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106834645"/>
    <dbReference type="KEGG" id="eai:106834645"/>
    <dbReference type="CTD" id="10539"/>
    <dbReference type="GeneTree" id="ENSGT00550000075030"/>
    <dbReference type="OMA" id="WAEPCKT"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990229">
        <property type="term" value="C:iron-sulfur cluster assembly complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044571">
        <property type="term" value="P:[2Fe-2S] cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03028">
        <property type="entry name" value="GRX_PICOT_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd02984">
        <property type="entry name" value="TRX_PICOT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000090">
        <property type="entry name" value="Glutaredoxin-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000165">
        <property type="entry name" value="glutaredoxin-3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000012">
        <property type="entry name" value="Monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033658">
        <property type="entry name" value="GRX_PICOT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004480">
        <property type="entry name" value="Monothiol_GRX-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00365">
        <property type="entry name" value="Grx4 family monothiol glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293">
        <property type="entry name" value="GLUTAREDOXIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10293:SF73">
        <property type="entry name" value="GLUTAREDOXIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Thioredoxin" evidence="14">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00078076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005021881.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="334" mass="37377" checksum="81A97BA76D1C1061" modified="2023-09-13" version="2">MAAGAAEAAAVVVEVGSARQFEELLRLRAKSLVVVHFWAPWAPQCAQMNDVMAELAKEHPQVSFVKLEAEAVPEVSEKYEISSVPTFLFFKNSQKVDRLDGAHAPELTKKVQRHASSGSFPPSGNEHPKEDLNLRLKKLTHAAPCMLFMKGTPQEPRCGFSKQMVEILNKHNIQFSSFDIFSDEEVRQGLKTYSSWPTYPQLYVSGELVGGLDIIKELEASEELDTICPKAPKLEERLKVLTNKASVMLFMKGNKQEAKCGFSKQILEILNSTGVDYETFDILEDEEVRQGLKAYSNWPTYPQLYVKGELVGGLDIVKELKENGELMPILKGEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M9X2</accession>
    <name>A0A8C4M9X2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Band 4.1-like protein 4A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Band 4.1-like protein 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Erythrocyte membrane protein band 4.1-like 4A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Erythrocyte membrane protein band 4.1-like 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Protein NBL4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">EPB41L4A</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Plays a role in the formation and organization of tight junctions during the establishment of polarity in epithelial cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of a complex composed of PALS1, CRB1 and EPB41L5. Within the complex, interacts (via FERM domain) with PALS1 (via HOOK domain) and with CRB1 (via intracellular domain). Interacts with CRB2 (via intracellular domain). Interacts with CRB3 (via intracellular domain).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Photoreceptor inner segment</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044636034.1">
        <property type="nucleotide sequence ID" value="XM_044780099.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M9X2"/>
    <dbReference type="Ensembl" id="ENSEAST00005024668.2">
        <property type="protein sequence ID" value="ENSEASP00005022737.2"/>
        <property type="gene ID" value="ENSEASG00005015263.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106832302"/>
    <dbReference type="KEGG" id="eai:106832302"/>
    <dbReference type="CTD" id="64097"/>
    <dbReference type="GeneTree" id="ENSGT00940000159623"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001917">
        <property type="term" value="C:photoreceptor inner segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13186">
        <property type="entry name" value="FERM_C_NBL4_NBL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17107">
        <property type="entry name" value="FERM_F1_EPB41L4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000116">
        <property type="entry name" value="band 4.1-like protein 4A isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000002">
        <property type="entry name" value="Band 4.1-like protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000003">
        <property type="entry name" value="Tyrosine-protein phosphatase non-receptor type 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030696">
        <property type="entry name" value="Band4.1-like4A_FERM_F1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF4">
        <property type="entry name" value="BAND 4.1-LIKE PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08736">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01195">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00661">
        <property type="entry name" value="FERM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="14">
        <location>
            <begin position="11"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="333"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="357"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="418"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="445"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="479"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="518"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="547"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="588"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="648"/>
            <end position="658"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00075138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00077094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005022737.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="686" mass="78760" checksum="C587C7DF6FB4E909" modified="2023-09-13" version="2">MGCFCAVPEEFYCEVLLLDESKLTLTSQQQGIKKSTKGSVVLDHVFRHINLVEIDYFGLRYCDRSHQTYWLDPAKTLAEHKELINTGPPYTLYFGIKFYAEDPCNLKEEITRYQFFLQVKQDVLQGRLPCPVNVAAQLGAYAIQSELGDYDPYKHTAGYVSEYRFVPDQKEELEEAIERIHKTLMGQAPSEAELNYLRTAKSLEMYGVDLHPVYGENKSEYFLGLTPVGVVVYKNKKQVGKYFWPRITKVHFKETQFELRVLGKDCNETSFFFEARSKTACKHLWKCSVEHHTFFRMPENESNSLSRRLSKFGSMSHKHRYSGRTALQMSRDLSIQLPRPDQNVARSRSKTYPKRIAQTQPAGSNSINRVTVNVENGENEGTTKIIAPSPIKSFKKAKNENSPDTQRSKSHAPWEENGPQSGLYNSPNDRTKSPKFPYTRRRNPSCGSDNDSVQPTRRRKAHNSGEDSDLKQRRRSRSRCNTSSGSESENSNREHRKKRNRIRQENDMVDSAPQWEAVLRRQKEKNQADSNNRRSRHRSRSRSPDIQAKEELWKHIQKELVDPSGLSEEQLKEIPYTKIETQGDPIRIRHSHSPRSYRQYRRSQCSDGERSVLSEVNSKTDLVPPLPVTRSSDAQGSGGSTVHQRRNGSKDSLIEEKSQTSTSSLAGKHTAKTIKTIQASRLKIET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MDZ1</accession>
    <name>A0A8C4MDZ1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA polymerase II subunit A C-terminal domain phosphatase SSU72</fullName>
            <shortName evidence="9">CTD phosphatase SSU72</shortName>
            <ecNumber evidence="9">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SSU72</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Protein phosphatase that catalyzes the dephosphorylation of the C-terminal domain of RNA polymerase II. Plays a role in RNA processing and termination.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SSU72 phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="9"/>
    <dbReference type="RefSeq" id="XP_014718631.1">
        <property type="nucleotide sequence ID" value="XM_014863145.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MDZ1"/>
    <dbReference type="SMR" id="A0A8C4MDZ1"/>
    <dbReference type="Ensembl" id="ENSEAST00005022970.2">
        <property type="protein sequence ID" value="ENSEASP00005021156.1"/>
        <property type="gene ID" value="ENSEASG00005014497.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106845226"/>
    <dbReference type="KEGG" id="eai:106845226"/>
    <dbReference type="CTD" id="29101"/>
    <dbReference type="GeneTree" id="ENSGT00390000010165"/>
    <dbReference type="OMA" id="PNCYEFG"/>
    <dbReference type="OrthoDB" id="57957at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008420">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000039">
        <property type="entry name" value="RNA polymerase II subunit A C-terminal domain phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000066">
        <property type="entry name" value="RNA polymerase II subunit A C-terminal domain phosphatase SSU72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006811">
        <property type="entry name" value="RNA_pol_II_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20383">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT A C-TERMINAL DOMAIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04722">
        <property type="entry name" value="Ssu72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005021156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="194" mass="22586" checksum="0E027A67A5AE92E6" modified="2022-01-19" version="1">MPSSPLRVAVVCSSNQNRSMEAHNILSKRGFSVRSFGTGTHVKLPGPAPDKPNVYDFKTTYDQMYNDLLRKDKELYTQNGILHMLDRNKRIKPRPERFQNCRDLFDLILTCEERVYDQVVEDLNSREQETCQPVHVINVDIQDNHEEATLGAFLICELCQCIQHTEDMENEIDELLQEFEEKSGRAFLHTVCFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MED3</accession>
    <name>A0A8C4MED3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Protein kinase cAMP-dependent type II regulatory subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PRKAR2B</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cAMP-dependent kinase regulatory chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014687328.1">
        <property type="nucleotide sequence ID" value="XM_014831842.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MED3"/>
    <dbReference type="Ensembl" id="ENSEAST00005027811.2">
        <property type="protein sequence ID" value="ENSEASP00005025613.1"/>
        <property type="gene ID" value="ENSEASG00005017464.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106825191"/>
    <dbReference type="KEGG" id="eai:106825191"/>
    <dbReference type="CTD" id="5577"/>
    <dbReference type="GeneTree" id="ENSGT00940000158160"/>
    <dbReference type="OMA" id="WSPPHHP"/>
    <dbReference type="OrthoDB" id="417078at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005952">
        <property type="term" value="C:cAMP-dependent protein kinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="C:centrosome"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097546">
        <property type="term" value="C:ciliary base"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043198">
        <property type="term" value="C:dendritic shaft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043197">
        <property type="term" value="C:dendritic spine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030552">
        <property type="term" value="F:cAMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004862">
        <property type="term" value="F:cAMP-dependent protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007612">
        <property type="term" value="P:learning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141162">
        <property type="term" value="P:negative regulation of cAMP/PKA signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00038">
        <property type="entry name" value="CAP_ED"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd12104">
        <property type="entry name" value="DD_RIIbeta_PKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000017">
        <property type="entry name" value="cAMP-dependent protein kinase type II regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.890.10:FF:000002">
        <property type="entry name" value="cAMP-dependent protein kinase type II-alpha regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000027">
        <property type="entry name" value="Protein kinase cAMP-dependent type II regulatory subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050503">
        <property type="entry name" value="cAMP-dep_PK_reg_su-like"/>
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    <dbReference type="InterPro" id="IPR012198">
        <property type="entry name" value="cAMP_dep_PK_reg_su"/>
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    <dbReference type="InterPro" id="IPR003117">
        <property type="entry name" value="cAMP_dep_PK_reg_su_I/II_a/b"/>
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    <dbReference type="InterPro" id="IPR018488">
        <property type="entry name" value="cNMP-bd_CS"/>
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    <dbReference type="InterPro" id="IPR000595">
        <property type="entry name" value="cNMP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018490">
        <property type="entry name" value="cNMP-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11635:SF156">
        <property type="entry name" value="CAMP-DEPENDENT PROTEIN KINASE TYPE II-BETA REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00027">
        <property type="entry name" value="cNMP_binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02197">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000548">
        <property type="entry name" value="PK_regulatory"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00103">
        <property type="entry name" value="CAMPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00100">
        <property type="entry name" value="cNMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00394">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51206">
        <property type="entry name" value="cAMP-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47391">
        <property type="entry name" value="Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00888">
        <property type="entry name" value="CNMP_BINDING_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00889">
        <property type="entry name" value="CNMP_BINDING_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50042">
        <property type="entry name" value="CNMP_BINDING_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13" id="KW-0114">cAMP</keyword>
    <keyword evidence="7 13" id="KW-0116">cAMP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="15">
        <location>
            <begin position="155"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Cyclic nucleotide-binding" evidence="15">
        <location>
            <begin position="277"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="353"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="362"/>
        </location>
        <ligand>
            <name>3',5'-cyclic AMP</name>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000548-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="419" mass="46277" checksum="6115825BDDCD93D0" modified="2022-01-19" version="1">MSIEIPAGLTELLQGFTVEVLRHQPADLLEFALQHFTRLQQESERKGAARCGQEARTWGDAGAAAGGGTPSKGVNFAEEPLHAESENGEEEEEEEAADAGAFPAPVINRFTRRASVCAEAYNPDEEEDDADSRIIHPKTDDQRNRLQEACKDILLFKNLDPEQMSQVLDAMFEKLVKEGEHVIDQGDDGDNFYVIDRGTFDIYVKCDGVGRCVGNYDNRGSFGELALMYNTPRAATITATSPGALWGLDRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEVSERLKVVDVIGTKVYSDGEQIIAQGDLADSFFIVESGEVKITMKRKGKSEAEENGAVEIARCSRGQYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQLVALFGTNMDIVEPTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MGW7</accession>
    <name>A0A8C4MGW7_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Regulator of G protein signaling 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">RGS11</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
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    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF14">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11830">
        <property type="entry name" value="DUF3350"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00640">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00462">
        <property type="entry name" value="PTB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01179">
        <property type="entry name" value="PID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PID" evidence="12">
        <location>
            <begin position="352"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="13">
        <location>
            <begin position="904"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="247"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="308"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="571"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="711"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="839"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="1185"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="263"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="594"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="677"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="736"/>
            <end position="746"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00072013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00081861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005022770.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1284" mass="144947" checksum="265E118778AE3B2D" modified="2023-09-13" version="2">MEPPSCIQDEPFPHPLEPEPGAPAQPGPGKPGEKRFRLWYVGGSCLDRRTTLPMLPWLMAEIRRRSQKPEAGGCGAPAAREVLLVLSAPLLRCVPAPGAGAAGGVGPAAAQPNPAVFIFEHKAQHISRFIHNSHDLTYFAYLIKAQPDDPESQMACHVFRATDPNQVPDVISSIRQYAFYNSQKFEVLYCGKVTVTHKKAPSSLIDDCMEKFSLHEQQRLRIQGEQRGADAGGEEPAVFEAEAPGSPAGILLEEGDGVTNTHPALAAPASQPGPSSSRGCFPERILEDSGFDEQQEFRSRCSSVTGVLQRKVHENSQKPQPRRRHASAPSHVQPSDSEKNRTMLFQVGRFEINLISPDTKSVVLEKNFKDISSCSQGIKHVDHFGFICRESPEPGLSQYTCYVFQCASESLVDEVMLTLKQAFSTAAALQSAKTQIKLCETCPMHSLHKLCERIEGLYPPRAKLVIQRHLSSLTDNEQADIFERVQKMKPVNDQEENELVILHLRQLCEAKQRTHIHIGEGPSTISNSTIPENATSSGRFKLDILKNKAKRSLTSSLENIFSRGANRMRGRLGSMDSFERSNSLASEKDYSPGDSPPGTPPASPLSSAWQSFPEEDSDSPQFRRRAHTFSHPPSSTKRKLNLQDGRTHGVRSPLLRQSSVEQCSIHPSVRRIHKESNSSSSLPSLHTSFSAPSFTAPSFLKSFYQNSGRLSPQYENDIRQDTASESSDGEGRKRTSSTCSNESLNVGGTPVTPRRISWRQRIFLRVASPMNKSPSAMQQQDGLDRNELLPLSPLAPTMEEEPLVIFMSDEDDPEKVEERNKSEELRSLWRKAIHQQILLLRMEKENQKLEASRDELQSRKVKLDYEEVGVCQKEVLITWDKKLLNCRSKIRCDIEDIHTSLKEGVPKSRRGEIWQFLALQYRLRHRLPNKQQPPDISYKELLKQLTAQQHAILVDLGRTFPTHPYFSAQLGAGQLSLFNLLKAYSLLDTEVGYCQGISFVAGVLLLHMSEEQAFEMLKFLMYDLGFRKQYRPDMMSLQIQMYQLSRLLHDYHRDLYNHLEENEISPSLYAAPWFLTLFASQFPLGFVARVFDIIFLQGTEVIFKVALSLLSSQETLIMECENFENIVEFLKSTLPDMNTSEMEKIITQVFEMDISKQLHAYEVEYHVLQDELQESSYACEDSEPMEKLERANNQLKRQNMDLLEKLQIAHAKIQTLESNMENLLTRETKLKSLIRTLEQEKIAYQKTVEQIRKLLPADALANCESLLRDLNCNPNNKAKTGNKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MKT5</accession>
    <name>A0A8C4MKT5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein</fullName>
            <shortName evidence="14">Cnt-b</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Centaurin-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ACAP1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. Required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.</text>
    </comment>
    <comment type="function">
        <text evidence="14">GTPase-activating protein for the ADP ribosylation factor family.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="14">GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Banana-shaped homodimer laterally assembling into tetramers, the tetramers further pack helically onto the membrane. Interacts with GTP-bound ARF6. Interacts with third cytoplasmic loop of SLC2A4/GLUT4. Interacts with CLTC. Interacts with GULP1. Forms a complex with GDP-bound ARF6 and GULP1. Interacts with ITGB1; required for ITGB1 recycling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endosome membrane</location>
            <topology evidence="14">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3). May mediate protein binding to PIP2 or PIP3 containing membranes.</text>
    </comment>
    <comment type="domain">
        <text evidence="14">The BAR domain mediates homodimerization, it can neither bind membrane nor impart curvature, but instead requires the neighboring PH domain to achieve these functions.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014716995.1">
        <property type="nucleotide sequence ID" value="XM_014861509.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MKT5"/>
    <dbReference type="Ensembl" id="ENSEAST00005030443.2">
        <property type="protein sequence ID" value="ENSEASP00005028032.2"/>
        <property type="gene ID" value="ENSEASG00005019039.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106844187"/>
    <dbReference type="KEGG" id="eai:106844187"/>
    <dbReference type="CTD" id="9744"/>
    <dbReference type="GeneTree" id="ENSGT00940000160289"/>
    <dbReference type="OrthoDB" id="10070851at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08852">
        <property type="entry name" value="ArfGap_ACAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07639">
        <property type="entry name" value="BAR_ACAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13250">
        <property type="entry name" value="PH_ACAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000062">
        <property type="entry name" value="Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000007">
        <property type="entry name" value="Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000020">
        <property type="entry name" value="Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000026">
        <property type="entry name" value="Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045258">
        <property type="entry name" value="ACAP1/2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004148">
        <property type="entry name" value="BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180:SF197">
        <property type="entry name" value="ARF-GAP WITH COILED-COIL, ANK REPEAT AND PH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23180">
        <property type="entry name" value="CENTAURIN/ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16746">
        <property type="entry name" value="BAR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 12 14" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4 14" id="KW-0967">Endosome</keyword>
    <keyword evidence="14" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0944">Nitration</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 13 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="16">
        <location>
            <begin position="265"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="17">
        <location>
            <begin position="405"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="606"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="12">
        <location>
            <begin position="639"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="525"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="15">
        <location>
            <begin position="537"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00056671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU369028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005028032.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="740" mass="81521" checksum="B22F251A92AD4E66" modified="2023-09-13" version="2">MTVKLDFEECLKDSPRFRASIELVEAEVSELETRLEKLLKLGNGLLESGRHYLAAGHAFIVGICDLARLGPPEPMMAECLDKFTESLRHKLDSHADLLDATQHTLQQQIQTLVKEGLRGFREARRDFWRGAESLEAALTHNAEVPRRRTQEAEEAGAALKTARAGYRGRALDYALQINVIEDKRKFDIMEFVLRLVEAQATHFQQGHEELSRLAQYRKDLGGQLHQLVLNSAREKRDMEQRHVLLKQKELGGEEPEPSLKEGPGGLVMEGHLFKRASNAFKTWSRRWFTIQSNQLVYQKKYKDPVTVVVDDLRLCTVKLCPDSERRFCFEVVSPSKSCLLQADSERLLQLWINAVQSSIATAFSQAHLDDSPRGSVQGSGHLAIGSAATMGCGGTTRGREPGGVGHVAAQVQSVDGNAQCCDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVRSLTLDSWEPELVKLMCELGNVVINQIYEARVEAMAVKKPGPSCSRQEKEAWIHAKYVEKKFLTKLPEIRGRRGGRGPPRGQPPVPPKPSIRPRPGSFGSKPEPPSDDLGSLHPGALLFRAAGHPPSLPTMADALAHGADVNWVNGGQENATPLIQATAANSLLACEFLLQNGANVNQADNHGRGPLHHATILGHTGLACLFLKRGADLGARDSEGRDPLTIAMEAANADIVTLLRLAKMREAEVAQGQAGDETYLDIFRDFSLMASDDPEKLSRRSYDLHTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MLF4</accession>
    <name>A0A8C4MLF4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kinetochore scaffold 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">KNL1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSEAST00005026280.2">
        <property type="protein sequence ID" value="ENSEASP00005024229.2"/>
        <property type="gene ID" value="ENSEASG00005016457.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00410000025918"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180019">
        <property type="term" value="C:Knl1/Spc105 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090266">
        <property type="term" value="P:regulation of mitotic cell cycle spindle assembly checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd22892">
        <property type="entry name" value="DRWD-C_Knl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22817">
        <property type="entry name" value="DRWD-N_Knl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21853">
        <property type="entry name" value="KNL1_NTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037388">
        <property type="entry name" value="Blinkin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043651">
        <property type="entry name" value="KNL1_MELT_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040850">
        <property type="entry name" value="Knl1_RWD_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16520">
        <property type="entry name" value="KINETOCHORE SCAFFOLD 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16520:SF3">
        <property type="entry name" value="KINETOCHORE SCAFFOLD 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18210">
        <property type="entry name" value="Knl1_RWD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19221">
        <property type="entry name" value="MELT"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Knl1 C-terminal RWD" evidence="3">
        <location>
            <begin position="2096"/>
            <end position="2249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="726"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1055"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1638"/>
            <end position="1668"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1780"/>
            <end position="1807"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="2080"/>
            <end position="2128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="726"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1638"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1649"/>
            <end position="1664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1780"/>
            <end position="1797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005024229.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="2351" mass="264352" checksum="9DEE8D6952713C51" modified="2023-09-13" version="2">MDGTSSEANEENDNTERPVRRRHSSILKPPRSPLQDLRNGNEIVQESNALRNKKSSRRVSFADTIKVFQTESYKKIVRKSEIEETEAGENALFIRNKNPEDNCCEITGMDTLLCAPIQIQRQQREISITEHNDEKKHANDQTVIFSDENQMDLTASHTVMITKGLLDCTKSETSTKIDTTSFLANLKLHTEDSSMKKELNFSMDQNTSSEKKINFNDFIKRLKEGKCKASSTGPDKENSEISVHAKESNRASSIHQMHVSVNTDENSSNMTRIFREQDGGMNFTQCHTANIQTLVPTSSEVSLREFKGDDITVYGNDFMDLTVNHTIQILPSDNLSEIENQTQNVVTDVTTGYGTIAAGKTTVFENKLNVPFQYPSLNPKGEVHVTRSHITGTETHTVTQPSNQDARTLTMIEESVCSSPAIQDNKTFFYSSCNNAMDLTKCLSSMREEKNLLKHDSNYSKMYPNPDAITPLIEKTTYSGEDSMDITKSHTVAIVNQIFKQDQTNVQIAAAPIPEKEMMFQNHITVSEVQEMNVNCSSVPHVSKERLQQSLTNPLSISLTDKKTEIFTGEDMDLTKSHTTNLESQVCLASYNLASENTSKSHSQSKSPLDEWEEMTKSHIEPSQQPNVISKNILTDTWGKEKDQVLKVSPYLDKDSPQSADINQDIATRHNIVYSSGDLKKQVMLANDRNTVSCEQSLFPATKPLFLSEGQSAMKNHNTAVNSHTVKSIPGQNSKLPEPLRKSLGNPTTECSHDKIIICSEEEQNMDLIKSHTIAIGFGPSEVQELGKTNLENTNSQIATVNRQIAVKVEKCNKSPIEKTEGVFISNNIMDVLEDKSVQKPGFLNEKQDVKICERKSIGRLKIDKAIVFSEGDENDMDITKSHTEEINHRPLLDGHDPHLVPLAGTSKTVLYTCGQDDVEVTRSHTTAIEYKTVSPGEIATKTMDKTVMFVDNHGELEMTKSHTALIDYRAKEKTVLPDRPNFELSKRKSLEEPKVTSIPAEENVFLPKNGESDHSAAKVSQLTLLGEWSNSGPTEKAVAFIADDNMEVSKSTIGKNDKDVQKPGFLNEPLSGKSQRRKSLRLKNDKTVVFSKNDTNDMDITQSCTVEINNQSALEDREDSRLVPFAGTSKTVLYTCGQDDMEITRSHTSALECKIVSADQITTRPVDKTVMFVGNHNGLEVTKSHTVFIDCQATEKILQECPKFGITKGKILGVSFPKDGSTFQEITKKQTLAVEDKNVLHTEQKHHMIPFVPTNTLSGCQGEIEMKFHSTAVDEEVMGKVVHQAYALEKAKIESCHLNSTDRRNVDFTNSHASAICGPSDNYSCLPNVISCFDNVEGNVMSLCDKDEKANNCPVQNDLTYANDLAGEYHLKSEGLAVSAPCSLLEKEKVIQANTKGQLDCVIKVLKDQDLIKEPQNLLVNQTLVYGQDLGEMTKLNSKRVSFKLPKDQMEAFVDNAEYSLKKTFVNDVCVASQPHLSTQLPPLPQQGQGLINKDATILSKAGNKNLNIVIGNSSVLTCENESKMLNNEKQFTIACKKELKEKIQTAKCNTAIDFHSNSDLTKQVIQTHANPREASDPVIASTAASFSSVKPNLNNLNGKTEEFLGFQTVHIPPSPEKLLELVNKAHNDMSIVQATEIHNNNIASSSVKDDRDEENKSSHNRTETNSVPLMTGVKDRVRRCSLGIFLPRLPNKRNCSVTGIDDLEQIPADTTDLNHLESQPVSIKDSSIGFVAAKLNLSPSQYINEENLPIYPGEINSSDSISIETEEKALIETYQKEISPSENKMEETCNSQKRTWVQEEEDDIPNEKKIRKSEIRVSDTAQDQEVLDHHAEGEIDKNATSVLIKSLSRTPSSCSSSLDSIKADGTSLDFSTHRNSQMESQFLRDTICEESLKEKLKDGRITIKEFFILLQVHILIQKPRQSNLPAKYTINTPPTPEDLMLSQYVYRPKIQIYREDCEVLRQKIEELKLSASNQDKLLTCVNKNLWEKMRHCSDEELKAFGIYLNKMKSHFTKMTKVFTHQGKVALYSKLVQSAQNEREKLHMRIDEMDNILKKIDNCLTAVEIETKNLEDEEKDNPVEEWDSEMRAAEKELEQLKTEEEVLQRNLLELEVQKEQTLAQIDFVQKQTNRTEELLDQLSLSEWDVIEWSDDQAVFTFLYDTIELTITFGEPVVGLSFLGNAHRKIVDLNFQFLLDEDKAPPSSLLVHKLIFQYIEEQESWKKKCTTQHQVPKMLQEISLVVSHCRLLGEEIEFLKRWGPNYNLMNIDVNNTELRLLFSSSAAFSKFEITLSLSAHYPSVPLTFSIQNHIGNIGQNEITTILSKVPLEDNYLKNVVKQVYRDLLQDCHFSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MLM7</accession>
    <name>A0A8C4MLM7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Folate receptor-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106836927</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the folate receptor family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MLM7"/>
    <dbReference type="Ensembl" id="ENSEAST00005031122.2">
        <property type="protein sequence ID" value="ENSEASP00005028650.2"/>
        <property type="gene ID" value="ENSEASG00005019484.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183144"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007342">
        <property type="term" value="P:fusion of sperm to egg plasma membrane involved in single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035036">
        <property type="term" value="P:sperm-egg recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004269">
        <property type="entry name" value="Folate_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018143">
        <property type="entry name" value="Folate_rcpt-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10517">
        <property type="entry name" value="FOLATE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10517:SF15">
        <property type="entry name" value="FOLATE RECEPTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03024">
        <property type="entry name" value="Folate_rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040501734" description="Folate receptor-like domain-containing protein" evidence="5">
        <location>
            <begin position="22"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Folate receptor-like" evidence="6">
        <location>
            <begin position="30"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005028650.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="251" mass="28718" checksum="4AF1673F259D51C3" modified="2023-09-13" version="1">MALPMTTQLLLLLVWVAAVQAALPRTDLNVCMDAKHYKQKPGPEDTLHEQCSPWKENSCCSLTTSQEAHKDISHLYRFNWDHCGRMEAVRKRHFIQDTCLCECSPNLGPWIQQVDQSWSKEQILDVPLCKEDCPCRWEDCHPSYTCKTNWHKGWNCTSGFNQCPVEAACRPFDFYFPTPEALCNEIWSHSYKVSNYSRGSGRCIQMWFDPAQGNPNEEVARFYAEAMNRAGLGGACSLLLCLALTLLWLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MMS9</accession>
    <name>A0A8C4MMS9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Protein O-mannosyl-transferase TMTC3</fullName>
            <ecNumber evidence="5">2.4.1.109</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Transmembrane O-mannosyltransferase targeting cadherins 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Transmembrane and tetratricopeptide repeat-containing 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">TMTC3</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. The 4 members of the TMTC family are O-mannosyl-transferases dedicated primarily to the cadherin superfamily, each member seems to have a distinct role in decorating the cadherin domains with O-linked mannose glycans at specific regions. Also acts as O-mannosyl-transferase on other proteins such as PDIA3. Involved in the positive regulation of proteasomal protein degradation in the endoplasmic reticulum (ER), and the control of ER stress response.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TMTC family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="5"/>
    <dbReference type="RefSeq" id="XP_044599662.1">
        <property type="nucleotide sequence ID" value="XM_044743727.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MMS9"/>
    <dbReference type="Ensembl" id="ENSEAST00005030841.2">
        <property type="protein sequence ID" value="ENSEASP00005028397.1"/>
        <property type="gene ID" value="ENSEASG00005019307.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106836516"/>
    <dbReference type="CTD" id="160418"/>
    <dbReference type="GeneTree" id="ENSGT00940000157538"/>
    <dbReference type="OMA" id="AKACFTR"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060255">
        <property type="term" value="P:regulation of macromolecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000175">
        <property type="entry name" value="transmembrane and TPR repeat-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000239">
        <property type="entry name" value="Transmembrane and TPR repeat-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000528">
        <property type="entry name" value="Transmembrane and TPR repeat-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013618">
        <property type="entry name" value="TMTC_DUF1736"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44395">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44395:SF1">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE TMTC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08409">
        <property type="entry name" value="TMTC_DUF1736"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 18" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="94"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="192"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="321"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="347"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="377"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1736" evidence="21">
        <location>
            <begin position="259"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="18">
        <location>
            <begin position="446"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="18">
        <location>
            <begin position="563"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="18">
        <location>
            <begin position="597"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="834"/>
            <end position="889"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="838"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="861"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="878"/>
            <end position="889"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR15508:SF9">
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    </dbReference>
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        </location>
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            <end position="211"/>
        </location>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_014685873.2">
        <property type="nucleotide sequence ID" value="XM_014830387.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044634882.1">
        <property type="nucleotide sequence ID" value="XM_044778947.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044634883.1">
        <property type="nucleotide sequence ID" value="XM_044778948.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044634884.1">
        <property type="nucleotide sequence ID" value="XM_044778949.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MYN4"/>
    <dbReference type="Ensembl" id="ENSEAST00005036053.2">
        <property type="protein sequence ID" value="ENSEASP00005033078.1"/>
        <property type="gene ID" value="ENSEASG00005022629.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106824205"/>
    <dbReference type="KEGG" id="eai:106824205"/>
    <dbReference type="CTD" id="203197"/>
    <dbReference type="GeneTree" id="ENSGT00390000011559"/>
    <dbReference type="OMA" id="YVTLWIN"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028054">
        <property type="entry name" value="DUF4481"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31193:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 268"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31193">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN C9ORF91"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14800">
        <property type="entry name" value="DUF4481"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005033078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="344" mass="37961" checksum="055124A309D32B38" modified="2022-01-19" version="1">MACEPQMEPGGAAGPMPPSSPSWSALPGGSPPGWGQGLHNGQVLTVLRIDNTCAPIAFDLGAAEEQLQTWGIQVPADQYRSLAESALLEPQVRRYIIYNSRPMRLAFAVVFYVVVWANIYSTSQLFALGNHWVAVLLVTLAAVSLTLALVLIFERHQRKANTNTDLRLAAANGALLRHRLLLGVTDTVEGCQSVIQLWFVYFDLENCVQFLADHVQEMKTNQESLLRRRLSQLCVVMETGVSPTAAEEPEDPMEEAPLLPSRPEPQERPLMQTELHQLVPEAEPEEMAQQLLAVFGGYYIRLLVTSQLPEAGGIRHMDSPRVPCPCQFIEAHILGTGCCPFLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N3J2</accession>
    <name>A0A8C4N3J2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">E3 ubiquitin-protein ligase TRIM32</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">RING-type E3 ubiquitin transferase TRIM32</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Tripartite motif-containing protein 32</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">TRIM32</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">It self-associates. Interacts with DTNBP1. Interacts with PIAS4/PIASY upon treatment with UVB and TNF-alpha. Interacts with AMBRA1; promoting activation of ULK1 through unanchored 'Lys-63'-linked polyubiquitin chains. Interacts with TICAM1 and TAX1BP1; these interactions target TICAM1 to TAX1BP1-mediated selective autophagic degradation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRIM/RBCC family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_014685891.1">
        <property type="nucleotide sequence ID" value="XM_014830405.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014685892.1">
        <property type="nucleotide sequence ID" value="XM_014830406.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014685893.1">
        <property type="nucleotide sequence ID" value="XM_014830407.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014685894.1">
        <property type="nucleotide sequence ID" value="XM_014830408.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4N3J2"/>
    <dbReference type="Ensembl" id="ENSEAST00005036037.2">
        <property type="protein sequence ID" value="ENSEASP00005033062.1"/>
        <property type="gene ID" value="ENSEASG00005022616.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005067949.1">
        <property type="protein sequence ID" value="ENSEASP00005061724.1"/>
        <property type="gene ID" value="ENSEASG00005022616.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106824216"/>
    <dbReference type="KEGG" id="eai:106824216"/>
    <dbReference type="CTD" id="22954"/>
    <dbReference type="GeneTree" id="ENSGT00940000160949"/>
    <dbReference type="OMA" id="LPTMFQL"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005863">
        <property type="term" value="C:striated muscle myosin thick filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017022">
        <property type="term" value="F:myosin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007014">
        <property type="term" value="P:actin ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061564">
        <property type="term" value="P:axon development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061824">
        <property type="term" value="P:cytosolic ciliogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045444">
        <property type="term" value="P:fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010994">
        <property type="term" value="P:free ubiquitin chain polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046716">
        <property type="term" value="P:muscle cell cellular homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902018">
        <property type="term" value="P:negative regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048147">
        <property type="term" value="P:negative regulation of fibroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902230">
        <property type="term" value="P:negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034144">
        <property type="term" value="P:negative regulation of toll-like receptor 4 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032897">
        <property type="term" value="P:negative regulation of viral transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000786">
        <property type="term" value="P:positive regulation of autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045787">
        <property type="term" value="P:positive regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030307">
        <property type="term" value="P:positive regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903886">
        <property type="term" value="P:positive regulation of chemokine (C-C motif) ligand 20 production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903883">
        <property type="term" value="P:positive regulation of interleukin-17-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050769">
        <property type="term" value="P:positive regulation of neurogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045666">
        <property type="term" value="P:positive regulation of neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045732">
        <property type="term" value="P:positive regulation of protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045862">
        <property type="term" value="P:positive regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051155">
        <property type="term" value="P:positive regulation of striated muscle cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903265">
        <property type="term" value="P:positive regulation of tumor necrosis factor-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070534">
        <property type="term" value="P:protein K63-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905161">
        <property type="term" value="P:protein localization to phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042594">
        <property type="term" value="P:response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034612">
        <property type="term" value="P:response to tumor necrosis factor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009411">
        <property type="term" value="P:response to UV"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044790">
        <property type="term" value="P:suppression of viral release by host"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001894">
        <property type="term" value="P:tissue homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034142">
        <property type="term" value="P:toll-like receptor 4 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd19806">
        <property type="entry name" value="Bbox1_TRIM32_C-VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14961">
        <property type="entry name" value="NHL_TRIM32_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16587">
        <property type="entry name" value="RING-HC_TRIM32_C-VII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000034">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000039">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000314">
        <property type="entry name" value="E3 ubiquitin-protein ligase TRIM32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001258">
        <property type="entry name" value="NHL_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047051">
        <property type="entry name" value="TRIM32_Bbox1_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000315">
        <property type="entry name" value="Znf_B-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25464:SF3">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR25464">
        <property type="entry name" value="TRIPARTITE MOTIF-CONTAINING PROTEIN 2-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01436">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00336">
        <property type="entry name" value="BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57845">
        <property type="entry name" value="B-box zinc-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
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    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51125">
        <property type="entry name" value="NHL"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50119">
        <property type="entry name" value="ZF_BBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 22" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 20" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="23">
        <location>
            <begin position="20"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="B box-type" evidence="24">
        <location>
            <begin position="95"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="21">
        <location>
            <begin position="358"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="21">
        <location>
            <begin position="415"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="21">
        <location>
            <begin position="464"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="21">
        <location>
            <begin position="562"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="repeat" description="NHL" evidence="21">
        <location>
            <begin position="616"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="22">
        <location>
            <begin position="139"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022786"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066261"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00073068"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00075992"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00076816"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005033062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="653" mass="71981" checksum="17433317500734F3" modified="2022-01-19" version="1">MAAAAASHLNLDALREVLECPICMESFTEEQLRPKLLHCGHTICRQCLEKLLASSINGVRCPFCSKITRITSLTQLTDNLTVLKIIDTAGLSEAVGLLMCRSCGRRLPRQFCRSCGVVLCEPCREADHQPPGHCTLPVKEAAEERRRDFGEKLARLRELMGELQRRKVALEGVSKDLQARYKAVLQEYGHEERRVQEELARSRKFFTGSLAEVEKSNSQVIEEQSYLLNIAEVQAVSRCDYFLAKIKQADVALLEETADEEEPELTASLPRELTLQDVELLKVGHVGPLQIGQAAKKPRTVNMEDSWAMEAAASAASTSVTFREMDLSPEEVVASPRASPAKQRGSETAANIQQCLFLKKMGAKGSTPGMFNLPVSLYVTSQGEVLVADRGNYRIQVFTRKGFLKEIRRSPSGIDSFVLSFLGADLPNLTPLSVAMNCHGLIGVTDSYDNSLKVYTLDGHCVACHRSQLSKPWGITALPSGQFVVTDVEGGKLWCFTVDRGAGVVKYNCLCSAVRPKFVTCDAEGTVYFTQGLGLNLENRQNEHHLEGGFSIGSVGPDGQLGRQISHFFSENEDFRCIAGMCVDARGDLIVADSSRKEILHFPKGGGYSVLIREGLTCPVGIALTPKGQLLVLDCWDHCIKIYSYHLRRYSTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PFJ6</accession>
    <name>A0A8C4PFJ6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Transient receptor potential cation channel subfamily M member 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">TRPM2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Na(+)(in) = Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:34963"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM2 sub-subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014686241.2">
        <property type="nucleotide sequence ID" value="XM_014830755.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044606842.1">
        <property type="nucleotide sequence ID" value="XM_044750907.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044606843.1">
        <property type="nucleotide sequence ID" value="XM_044750908.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044606845.1">
        <property type="nucleotide sequence ID" value="XM_044750910.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044606846.1">
        <property type="nucleotide sequence ID" value="XM_044750911.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044606847.1">
        <property type="nucleotide sequence ID" value="XM_044750912.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044606848.1">
        <property type="nucleotide sequence ID" value="XM_044750913.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005001930.2">
        <property type="protein sequence ID" value="ENSEASP00005001733.2"/>
        <property type="gene ID" value="ENSEASG00005000375.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106824449"/>
    <dbReference type="KEGG" id="eai:106824449"/>
    <dbReference type="CTD" id="7226"/>
    <dbReference type="GeneTree" id="ENSGT00940000156404"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015278">
        <property type="term" value="F:intracellularly gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072571">
        <property type="term" value="F:mono-ADP-D-ribose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070301">
        <property type="term" value="P:cellular response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071502">
        <property type="term" value="P:cellular response to temperature stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002407">
        <property type="term" value="P:dendritic cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097028">
        <property type="term" value="P:dendritic cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051289">
        <property type="term" value="P:protein homotetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051489">
        <property type="term" value="P:regulation of filopodium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051209">
        <property type="term" value="P:release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033194">
        <property type="term" value="P:response to hydroperoxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014074">
        <property type="term" value="P:response to purine-containing compound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001659">
        <property type="term" value="P:temperature homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071577">
        <property type="term" value="P:zinc ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03670">
        <property type="entry name" value="NUDIX_ADPRase_Nudt9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000047">
        <property type="entry name" value="Transient receptor potential cation channel subfamily M member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.450">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050927">
        <property type="entry name" value="TRPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057366">
        <property type="entry name" value="TRPM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041491">
        <property type="entry name" value="TRPM_SLOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800:SF2">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL SUBFAMILY M MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13800">
        <property type="entry name" value="TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL, SUBFAMILY M, MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18139">
        <property type="entry name" value="LSDAT_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25969">
        <property type="entry name" value="NUDT9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25508">
        <property type="entry name" value="TRPM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="6" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="752"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="791"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="869"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="933"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1022"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="domain" description="Nudix hydrolase" evidence="20">
        <location>
            <begin position="1353"/>
            <end position="1497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1205"/>
            <end position="1236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1224"/>
            <end position="1236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="ARBA" id="ARBA00022568"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
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            <dbReference type="ARBA" id="ARBA00022673"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
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    </evidence>
    <evidence type="ECO:0000256" key="8">
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    </evidence>
    <evidence type="ECO:0000256" key="9">
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    </evidence>
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    </evidence>
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    <evidence type="ECO:0000256" key="14">
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            <dbReference type="ARBA" id="ARBA00023303"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
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            <dbReference type="ARBA" id="ARBA00036239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00070987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001733.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1502" mass="171318" checksum="BCA02FC13A5F51D4" modified="2023-09-13" version="2">MEPSTPWKAGSKQEEGFGGQPRRVADLGAGPNLRRSDSSLPKNRRSQYLLGNCEKQKNLSSWIPENIKKKECMYFVESSKLSDAGKVVCECGYTHEQHLEEATWPCAFQGKEWDPKKHVQEMPTDAFGDIAFMGLGQKVGKYVRLSQDTPSSVIYHLITQHWGLDVPNLLISVTGGAKDFNMKPRLKNVFRRGLVKVAQTTGAWIITGGTHTGVMKQVGEAVRDFSLSSSYNEGEVVTIGIATWGTLHNREGLIHPTGGFPAEYIVDEDGQGHLTCLDSNHSHFILVDDGTHGRYGVEIPLRTKLEKFISEQTKERGGVAIKIPIVCVVLEGGPGTLHTIYNAITNGTPCVIVEGSGRVADVIAQVAGLPISEITISLIQQKLSMFFQEMFETFTEGRIVEWTKKIQDIVRRRQLLTIFREGKDGQQDVDVAILQALLKASRSHDHFGHENWDHQLKLAVAWNRVDIARSEIFTDERQWKPSELHPMMTAALISNKPEFVKLFLEHGVRLKELVTWDSLLYLYKNLDPSCLFHCKLQKVLAEEPERLACAPAAPRVQMHHVAQVLRELLGDSTQLLYPRPQTSDRPRLTLPVPHIKLNVQGVSLRSLYKHSSGHVAFTMDPVRDLLIWAIVQNRRELAEIIWDQSQDCIAAALACTKILKELSKEEQDTDSSEEMLALADEYEHRAIGVFTECYRKDEERAQKLLIRVSEAWGKTTCLQLALEAKDMKFVSHGGIQAFLTKVWWGHLCVDNGLWRVILCMLAFPLLYTDLTSFRERRLQAVRGLPRIRAFFTAPVVIFHLNILWYFVFLCLFAYVLMVDFQTTPSCCEYLLYFWLFSLVCEELRQLFYDPAGCGLRKTVFMYFSDFWNKLDIAAILLFIAGLTCRLIPALLYSGRVILSLDFIMFCLRLMHIFTISKTLGPKIIIMKRMMKDVFFFLFLLAVWVVSFGVAKQAILIHNESRVDWIFRGVVYQSYLTIFGEIPAYIDGVNFSLDHCSPNGTDPYKPKCPESDATRQAPAFPQWLTVILLCLYLLFANILLLNLLIAMFNYTFQQVQEHTDQIWKFQRHDLIEEYHSRPPAPPPFILLSHLHLFIRRVVLKIPAKRHKQLKNKLEKNEEAALLAWEIYLKENYLQNQQCQRTQRPEQKIQDISNKVDTMVDLLEMERVKRSGSMEQRLASLEEQVAQMAGALHWMVKTLRDGGFGLEEGVPTLAPQKASEGQDPELDGRQKAEDPGDAHHVNARHLLYPSSRVMRFPVPNEKVPWETEFLIYDPPFYTAERKDKDTVDPVGSTLEPLSGISYNALDGPVDRRSSHGPYAVRDGLPRNPMGRTGLWGRGDLTYFGPNHTLQPVVTRWRRNQDGAICRKSIKKMLEVLVVKRPLSEHWALPGGSREPGETLPRKLKQVLRREFWPSFESLLTQGVEVYKGYVDDPRNTDNAWIETVAVSIHFPDQSDVELKRLNSHLQPCDPGMSVRWQVVDKCIPLYANHKTILQKVATLFGAYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PHD9</accession>
    <name>A0A8C4PHD9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Ephrin type-B receptor 1</fullName>
            <ecNumber evidence="4">2.7.10.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="36" type="primary">EPHB1</name>
    </gene>
    <organism evidence="36 37">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="36 37" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="36" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="36" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="24">Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Interacts with EPHB6; transphosphorylates EPHB6 to form an active signaling complex. Interacts with PICK1. Interacts (through Tyr-594) with NCK1 (via SH2 domain); activates the JUN cascade to regulate cell adhesion. The ligand-activated form interacts (through Tyr-928) with GRB7 and GRB10 (via SH2 domains). The ligand-activated form interacts (residues within the catalytic domain) with GRB2 (via SH2 domain). Interacts with GRB2, SHC1 and SRC; activates the MAPK/ERK cascade to regulate cell migration. Interacts with CBL; regulates receptor degradation through ubiquitination. Interacts with ACP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Early endosome membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.7.10.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4PHD9"/>
    <dbReference type="SMR" id="A0A8C4PHD9"/>
    <dbReference type="Ensembl" id="ENSEAST00005005640.2">
        <property type="protein sequence ID" value="ENSEASP00005005156.2"/>
        <property type="gene ID" value="ENSEASG00005003859.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155297"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032433">
        <property type="term" value="C:filopodium tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS51550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005156.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="984" mass="109915" checksum="2F69E0AB16B8805F" modified="2023-09-13" version="2">MALDCLLLLLLASAVAAMEETLMDTRTATAELGWTANPASGWEEVSGYDENLNTIRTYQVCNVFEPNQNNWLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTIAADESFSQVDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETMTGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGMFKANQEAEGCSHCPSNSRSPSEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGGRDDVTYNIICKKCRADRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAVNGVSSKSPFPPQHVSVNITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNTARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTLTDDDYKSELREQLPLIAGSAAAGVVFVVSLVAISIVCSRKRAYSKEAVYSDKLQHYSTGRGSPGMKIYIDPFTYEDPNEAVREFAKEIDVSFVKIEEVIGAGEFGEVYKGRLKLPGKREIYVAIKTLKAGYSEKQRRDFLSEASIMGQFDHPNIIRLEGVVTKSRPVMIITEFMENGALDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLAEMNYVHRDLAARNILVNSNLVCKVSDFGLSRYLQDDTSDPTYTSSLGGKIPVRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPMDCPAALHQLMLDCWQKDRNSRPRFAEIVNTLDKMIRNPASLKTVATITAVPSQPLLDRSIPDFTAFTTVDDWLSAIKMVQYRDSFLTAGFTSLQLVTQMTSEDLLRIGVTLAGHQKKILNSIHSMRVQMNQSPTAMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PLP5</accession>
    <name>A0A8C4PLP5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Tubulin gamma chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TUBG1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 9">Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers. Gamma-tubulin is a key component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation. The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PLP5"/>
    <dbReference type="Ensembl" id="ENSEAST00005015634.2">
        <property type="protein sequence ID" value="ENSEASP00005014389.2"/>
        <property type="gene ID" value="ENSEASG00005010016.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156957"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045177">
        <property type="term" value="C:apical part of cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031252">
        <property type="term" value="C:cell leading edge"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000794">
        <property type="term" value="C:condensed nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990498">
        <property type="term" value="C:mitotic spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097730">
        <property type="term" value="C:non-motile cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000242">
        <property type="term" value="C:pericentriolar material"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005827">
        <property type="term" value="C:polar microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000212">
        <property type="term" value="P:meiotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02188">
        <property type="entry name" value="gamma_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000003">
        <property type="entry name" value="Tubulin gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000010">
        <property type="entry name" value="Tubulin gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002454">
        <property type="entry name" value="Gamma_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01164">
        <property type="entry name" value="GAMMATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="11">
        <location>
            <begin position="48"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="12">
        <location>
            <begin position="249"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="426"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="431"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="454"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014389.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="538" mass="59625" checksum="5BF2799346EF64B6" modified="2023-09-13" version="2">MPREIITLQLGQCGNQIGFEFWKQLCAEHGISPEGIVEEFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVIHSILNSPYAKLYNPENIYLSEHGGGAGNNWASGFSQGEKIHEDIFDIIDREADGSDSLEGFVLCHSIAGGTGSGLGSYLLERLNDRYPKKLVQTYSVFPNQDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLPSAHRVSGLMMANHTSISSPSLGSLALGAILFLEVLCDPCFLHTPSSLSGPVASMTSCESGRPSWSSSARRTSSRRTLTSWTHPGRSCSSSSTSIMQPHGRTTSPGAPRSSESPKTGTLICLTGWPRPCLTDHSLRAQIRDLTHLFLINTHTRTLLAWGRVYFSSYEIIYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PMI3</accession>
    <name>A0A8C4PMI3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Zinc finger protein 410</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">ZNF410</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Transcription factor that binds to the sequence motif 5'-CATCCCATAATA-3', and is specifically required to silence expression of fetal hemoglobin in adult erythroid cells. Prevents expression of fetal hemoglobin genes HBG1 and HBG2 through CHD4: acts as a direct transcriptional activator of CHD4, a central component of the NuRD complex that represses transcription of fetal hemoglobin genes HBG1 and HBG2 in erythroid cells. May also activate transcription of matrix-remodeling genes such as MMP1 during fibroblast senescence. May activate transcription of the gap junction gene GJC1, perhaps in response to increasing glucose. However, recent studies suggest that ZNF410 is dedicated to regulate expression of a single gene: CHD4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with CDKN2A/p14ARF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014835646.3"/>
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    <dbReference type="RefSeq" id="XP_014691133.1">
        <property type="nucleotide sequence ID" value="XM_014835647.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070370148.1">
        <property type="nucleotide sequence ID" value="XM_070514047.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A8C4PMI3"/>
    <dbReference type="Ensembl" id="ENSEAST00005018231.2">
        <property type="protein sequence ID" value="ENSEASP00005016777.1"/>
        <property type="gene ID" value="ENSEASG00005011626.2"/>
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    <dbReference type="GeneID" id="106827676"/>
    <dbReference type="KEGG" id="eai:106827676"/>
    <dbReference type="CTD" id="57862"/>
    <dbReference type="GeneTree" id="ENSGT00940000158098"/>
    <dbReference type="OMA" id="XNDRSFI"/>
    <dbReference type="OrthoDB" id="5977959at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:PcG protein complex"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0010629">
        <property type="term" value="P:negative regulation of gene expression"/>
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        <property type="term" value="P:oxygen transport"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="Putative zinc finger protein 143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000221">
        <property type="entry name" value="Zinc finger protein 410"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000462">
        <property type="entry name" value="Zinc finger protein 410"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000441">
        <property type="entry name" value="zinc finger protein 410 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
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        <property type="entry name" value="Znf_C2H2_sf"/>
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        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR PROTEIN YY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14003:SF24">
        <property type="entry name" value="ZINC FINGER PROTEIN 410"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
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    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 18" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="219"/>
            <end position="248"/>
        </location>
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        <location>
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            <begin position="339"/>
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            <begin position="84"/>
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            <begin position="187"/>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
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        </source>
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            <dbReference type="Ensembl" id="ENSEASP00005016777.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Vertebrata</taxon>
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            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Yue"/>
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                <person name="Liu"/>
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                <person name="D.D."/>
                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        </location>
    </feature>
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            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="27"/>
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    <evidence type="ECO:0000256" key="1">
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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</entry>
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    <name>A0A8C4PPK6_EQUAS</name>
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            <fullName evidence="4">DNase II-like acid DNase</fullName>
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        <alternativeName>
            <fullName evidence="4">DNase2-like acid DNase</fullName>
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        <alternativeName>
            <fullName evidence="4">Deoxyribonuclease II beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Endonuclease DLAD</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">DNASE2B</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Hydrolyzes DNA under acidic conditions. Does not require divalent cations for activity. Participates in the degradation of nuclear DNA during lens cell differentiation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.</text>
            <dbReference type="EC" id="3.1.22.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the DNase II family.</text>
    </comment>
    <dbReference type="EC" id="3.1.22.1" evidence="4"/>
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        <property type="nucleotide sequence ID" value="XM_014830037.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A8C4PPK6"/>
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        <property type="gene ID" value="ENSEASG00005014162.2"/>
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        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
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    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0004531">
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006309">
        <property type="term" value="P:apoptotic DNA fragmentation"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09192">
        <property type="entry name" value="PLDc_DNaseII_beta_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004947">
        <property type="entry name" value="DNase_II"/>
    </dbReference>
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        <property type="entry name" value="DEOXYRIBONUCLEASE II"/>
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    </dbReference>
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    </dbReference>
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    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="1"/>
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        </location>
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            <begin position="24"/>
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    </evidence>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043520">
        <property type="entry name" value="SPT21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47500">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47500:SF1">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED PROTEIN 21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="442"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="633"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="49"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="230"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="248"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="638"/>
            <end position="647"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005023306.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="657" mass="71371" checksum="3D7013902E974A67" modified="2023-09-13" version="2">MSSRKQPLSAPGGPEKGPGHREASEEGPAEVRTQEQRHLTGPGEKRSPRGPQEGPGEPQAGQDQATPGSELGMLPSGVPAAHAGMKQLGSGKETEDEQRGQQTPGTVEGEAPESCQQGLECQPTPGHQAAEESDVEPPAEPCCPLDSCGQQLGDKPPREAGAPHIRPRGALPESGPGRYEDSSQEAMPLMPTANLEEKKANCFLPSMPAPNTPKEGGRAVETQPAAGPDPPREVRDAGERMELDHVQKQPQEPTVATGAQNPGSLCQGFMKCLLEVEEEEEEATHRKATKAQALPNWKSPRAPTPVPNSALGLPLTLPQTPPMAPSWARPPAPGPVPASVGAPVLASVPATALPASAPDLGWRRTEFLPQSCQRSLSYARARQEPEDRCLLKLHQNWEERAEDHLTLKQEEAFRSYFEIFNGHGEVDAQSLENILLLVGISLMPAQVEDALMSADIDGDGHVGFKDFLAVMTDTKRFFCSVEQNALTDMAPPNPHTLFFEILSLLVEMMALPEAALEEITNYYQKKLKEGTCKAREMESAIGRLRARKKLPYNPQQADTVEVPEGRVLRILSRLKQQDYAANLQSPYAQVPCIPLCPRLDKKTVHRKQGSHYVLDQCTPPSLAPDIRSLFFQSGSQGNREHGSDSRKWLSSVPARTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PU26</accession>
    <name>A0A8C4PU26_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Major facilitator superfamily domain containing 6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MFSD6</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. MFSD6 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044624487.1">
        <property type="nucleotide sequence ID" value="XM_044768552.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070364354.1">
        <property type="nucleotide sequence ID" value="XM_070508253.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PU26"/>
    <dbReference type="Ensembl" id="ENSEAST00005033537.2">
        <property type="protein sequence ID" value="ENSEASP00005030845.1"/>
        <property type="gene ID" value="ENSEASG00005021013.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106828804"/>
    <dbReference type="CTD" id="54842"/>
    <dbReference type="GeneTree" id="ENSGT00530000063599"/>
    <dbReference type="OMA" id="LMLLYHG"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042590">
        <property type="term" value="P:antigen processing and presentation of exogenous peptide antigen via MHC class I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17335">
        <property type="entry name" value="MFS_MFSD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000136">
        <property type="entry name" value="Major facilitator superfamily domain-containing protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000229">
        <property type="entry name" value="Major facilitator superfamily domain-containing protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024989">
        <property type="entry name" value="MFS_assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051717">
        <property type="entry name" value="MFS_MFSD6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16172">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 6-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12832">
        <property type="entry name" value="MFS_1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="295"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="339"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="459"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="521"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="550"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="590"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="614"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily associated" evidence="8">
        <location>
            <begin position="72"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="413"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="675"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="744" mass="83157" checksum="BEE032D1B062B30C" modified="2022-01-19" version="1">MATDDKVAILTDDEEEQKRKYVLADPFNGISRDPEAPSNETPSSTETAAVPEEEIDWMEKHCVKINNDLLISKVFYFFFYSAYGSLYPLLPVYYKQLGMSPSQSGLLVGIRYFIEFCSAPFWGVIADRFKKGKIVLLFSLLCWVLFNLGIGFVKPATLRCVPKIPPTARPTNASHLLTVLPTNASLLSSITTSPRMREKRNLPSSSWPMMLEAEPNITETVIFSTAANKTSAPTLQPPTDEITDHVMGLTSNPSTATPAHPGSVTREITTTVVTTTKSLPSDQVTLVYDQQEVEAIFLVILMVVIIGEFFSASSVTIVDTVTLQYLGKHRDRYGLQRMWGSLGWGLAMLSVGIGIDYTHMEVLIDGKGCKPPEYRNYQIVFIVFGVLMTMALIVATQFRFRYNHFKNNENKGKEVEIPQVERNNSTESSEETPTTMSHSQAFNFWDLIRLLCSVQYGSVLFVAWFMGFGYGFVFTFLYWHLEDLNGTTTLFGVCSVLSHVSELTAYFFSHKLIELIGHIRVLYIGLACNTARYIYISYLENAWTVLPMEVLQGVTHAAIWAACISYLSAAVPPELRTSAQGILQGLHLGLGRGCGAMIGGVLVNYFGAAATFRGIGMACLVILLLFALIQWLAVPEEEEDKTMLAERIPVPSSPVPIATIDLVQQQTEDVMPRIEPRLPPKKTKHQEEQEDVNKPAWGVSSSPWVTFVYALYQIKEMMQLTRDNRASEIQPLQLHIGKEMKGDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PUL8</accession>
    <name>A0A8C4PUL8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">DERPC proline and glycine rich nuclear protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">DERPC</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
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                <person name="A."/>
                <person name="Amills"/>
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                <person name="D.D."/>
                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="DECREASED EXPRESSION IN RENAL AND PROSTATE CANCER PROTEIN"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
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        </location>
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    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="125"/>
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        </location>
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        <location>
            <begin position="241"/>
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        <location>
            <begin position="458"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
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        <recommendedName>
            <fullName evidence="3 10">Origin recognition complex subunit 4</fullName>
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    </protein>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
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                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Fang"/>
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                <person name="Zhao"/>
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                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
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                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="12" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="10">Component of the origin recognition complex (ORC) that binds origins of replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Interacts with DBF4. Interacts with POLQ.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ORC4 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_044768968.2"/>
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        <property type="nucleotide sequence ID" value="XM_070507670.1"/>
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        <property type="gene ID" value="ENSEASG00005023452.2"/>
    </dbReference>
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    <dbReference type="KEGG" id="eai:106825090"/>
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    <dbReference type="GeneTree" id="ENSGT00390000016542"/>
    <dbReference type="OMA" id="AFTFQRN"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000649">
        <property type="entry name" value="Origin recognition complex subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016527">
        <property type="entry name" value="ORC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032705">
        <property type="entry name" value="ORC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12087">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12087:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14629">
        <property type="entry name" value="ORC4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF007858">
        <property type="entry name" value="ORC4"/>
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="59"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR007858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005034356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="436" mass="50449" checksum="78A1ACE50E2F813C" modified="2022-01-19" version="1">MSSRKSKSNNLKHSECLSQVQRILRERFCHQSLHSNLFGVQVQYKHLIELLKRTAIYGESNSVLIIGPRGSGKTMLINHALKELMEVEEVSENVLQVHLNGLLQINDNIALKEITRQLNLENVVGDKVFGSFAENLSFLLEALKKGDRTSSCPVIFILDEFDLFAHHKNQTLLYNLFDISQSAQTPVAVVGLTCRLDILELLEKRVKSRFSHRQIHLMNSFGFPQYLKIFKEQLSLPAEFPDKLFAEKWNENVQCLSEDRSVREVLQKHFNVSKNLRSLHMLLMLALNRVTSSHPFMTAADLMEANQLCSMDSKANIVHGLSVLEICLIIAMKHLNDIYEEEPFNFQMVYNEFQKFVQRKAHSVYNFEKPVVMKAFEHLQQLELIRPMERTSVNAQREYQLMKLLLDNTQIMNALQKYPNCPTDVRQWATSSLSWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I766</accession>
    <name>A0A9L0I766_EQUAS</name>
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        <recommendedName>
            <fullName evidence="22">Peroxisome biogenesis factor 2</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
            <ecNumber evidence="21">2.3.2.36</ecNumber>
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            <fullName evidence="18">Peroxin-2</fullName>
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        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Peroxisome assembly factor 1</fullName>
        </alternativeName>
    </protein>
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        <name evidence="28" type="primary">PEX2</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
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                <person name="Z."/>
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                <person name="Y."/>
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                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
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                <person name="G."/>
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                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
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                <person name="C."/>
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                <person name="Y."/>
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                <person name="G."/>
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                <person name="M."/>
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                <person name="J."/>
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                <person name="S."/>
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                <person name="A."/>
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                <person name="M."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
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            <text>[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-S-ubiquitinyl-L-cysteine.</text>
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        <text evidence="3">Protein modification; protein ubiquitination.</text>
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    <comment type="subunit">
        <text evidence="20">Component of the PEX2-PEX10-PEX12 retrotranslocation channel, composed of PEX2, PEX10 and PEX12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the pex2/pex10/pex12 family.</text>
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        <property type="component" value="Chromosome 3"/>
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        <property type="entry name" value="Znf_RING_CS"/>
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        <property type="entry name" value="ZF_RING_2"/>
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    <keyword evidence="15 26" id="KW-0472">Membrane</keyword>
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    <keyword evidence="16" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 25" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="195"/>
            <end position="213"/>
        </location>
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        <location>
            <begin position="244"/>
            <end position="283"/>
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        <source>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
    <sequence length="305" mass="35162" checksum="DCFC52E7B52E48D6" modified="2023-09-13" version="1">MASREEGTKRTSRVLRISQLDALELNKALEQLVWSQFTQCFHGFKPGLLARFEPEVKAFLWLFLWRFTIYSKNATVGQSVLNIQYKNDFSPNPRYQPPSRNQKLWYAVCTIGGKWLEERCYDLFRNPHLASFRKAKQCVNFMVGLLKLGGLMNFLIFLQRGKFATLTERLLGIRSVFCKPQNIREVGFEYMNRELLWHGFAEFLIFLLPLINIQKLKAKLSSWCIPLTGAPNGDSTLATSGRQCSLCGEWPTMPHTIGCEHIFCYYCVKSSFLYDMYFTCPKCGTEVHSLQPLKSGIEMSEVNAL</sequence>
</entry>
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    <name>A0A9L0I7F4_EQUAS</name>
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        <submittedName>
            <fullName evidence="8">Phospholipid phosphatase 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PLPP5</name>
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    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
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                <person name="Ju"/>
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                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
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                <person name="Hou"/>
                <person name="M."/>
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                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="Guo"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
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    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 8" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="744"/>
            <end position="778"/>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IC79</accession>
    <name>A0A9L0IC79_EQUAS</name>
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        <recommendedName>
            <fullName evidence="10">Interleukin-1 receptor-associated kinase 1-binding protein 1</fullName>
        </recommendedName>
    </protein>
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        <name evidence="11" type="primary">IRAK1BP1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the IRAK1-dependent TNFRSF1A signaling pathway that leads to NF-kappa-B activation and is required for cell survival. Acts by enhancing RELA transcriptional activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the IRAK1BP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014684852.1">
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        <property type="gene ID" value="ENSEASG00005018565.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 24"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006955">
        <property type="term" value="P:immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043123">
        <property type="term" value="P:positive regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.170:FF:000002">
        <property type="entry name" value="Interleukin-1 receptor-associated kinase 1-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2970:FF:000002">
        <property type="entry name" value="interleukin-1 receptor-associated kinase 1-binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.170">
        <property type="entry name" value="Protein of unknown function (DUF541), domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2970">
        <property type="entry name" value="Protein of unknown function (DUF541), domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030312">
        <property type="entry name" value="IRAK1BP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007497">
        <property type="entry name" value="SIMPL/DUF541"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18842">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR-ASSOCIATED KINASE 1-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18842:SF2">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR-ASSOCIATED KINASE 1-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04402">
        <property type="entry name" value="SIMPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
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        </source>
    </evidence>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="X. and Zhong"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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            <authorList>
                <consortium name="Ensembl"/>
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                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the histone H2A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IG59"/>
    <dbReference type="Ensembl" id="ENSEAST00005082631.1">
        <property type="protein sequence ID" value="ENSEASP00005039176.1"/>
        <property type="gene ID" value="ENSEASG00005036023.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153092"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
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    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00074">
        <property type="entry name" value="HFD_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000103">
        <property type="entry name" value="Histone H2A type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002119">
        <property type="entry name" value="Histone_H2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032454">
        <property type="entry name" value="Histone_H2A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032458">
        <property type="entry name" value="Histone_H2A_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23430">
        <property type="entry name" value="HISTONE H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16211">
        <property type="entry name" value="Histone_H2A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00620">
        <property type="entry name" value="HISTONEH2A"/>
    </dbReference>
    <dbReference type="SMART" id="SM00414">
        <property type="entry name" value="H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00046">
        <property type="entry name" value="HISTONE_H2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5 14" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="13 14" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="16">
        <location>
            <begin position="7"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Histone H2A C-terminal" evidence="17">
        <location>
            <begin position="92"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF16211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="134" mass="14519" checksum="B4F1B083DCED5397" modified="2023-09-13" version="1">MSGRGKQGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYAERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGRVTIAQGGVLPNIQAVLLPKKTESHHKAKGKKAIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IL07</accession>
    <name>A0A9L0IL07_EQUAS</name>
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        <recommendedName>
            <fullName evidence="18">Sarcolemmal membrane-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">SLMAP</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Associates with the striatin-interacting phosphatase and kinase (STRIPAK) core complex, forming the extended (SIKE1:SLMAP)STRIPAK complex. The (SIKE1:SLMAP)STRIPAK complex dephosphorylates STK3 leading to the inhibition of Hippo signaling and the control of cell growth. May play a role during myoblast fusion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homodimer. Interacts with myosin. Interacts with SIKE1 and both associate with the STRIPAK core complex composed of PP2A catalytic and scaffolding subunits, the striatins (PP2A regulatory subunits), the striatin-associated proteins MOB4, STRIP1 and STRIP2, PDCD10 and members of the STE20 kinases, such as STK24 and STK26. Interacts (via FHA domain) with STK3 (when phosphorylated); the interaction associates STK3 with the STRIPAK complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cell membrane</location>
            <location evidence="15">Sarcolemma</location>
            <topology evidence="15">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Mitochondrion membrane</location>
            <topology evidence="13">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the SLMAP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070349330.1">
        <property type="nucleotide sequence ID" value="XM_070493229.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IL07"/>
    <dbReference type="Ensembl" id="ENSEAST00005071753.1">
        <property type="protein sequence ID" value="ENSEASP00005041630.1"/>
        <property type="gene ID" value="ENSEASG00005001149.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106848660"/>
    <dbReference type="CTD" id="7871"/>
    <dbReference type="GeneTree" id="ENSGT00940000157660"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042383">
        <property type="term" value="C:sarcolemma"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900825">
        <property type="term" value="P:regulation of membrane depolarization during cardiac muscle cell action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21911">
        <property type="entry name" value="CC1_SLMAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22679">
        <property type="entry name" value="FHA_SLMAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000003">
        <property type="entry name" value="sarcolemmal membrane-associated protein isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051176">
        <property type="entry name" value="Cent_Immune-Sig_Mod"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 170 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15715:SF22">
        <property type="entry name" value="SARCOLEMMAL MEMBRANE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
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    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
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        <location>
            <begin position="763"/>
            <end position="782"/>
        </location>
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        <location>
            <begin position="28"/>
            <end position="85"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="398"/>
            <end position="429"/>
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    </feature>
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            <begin position="172"/>
            <end position="199"/>
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            <begin position="272"/>
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            <begin position="441"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0ITU4</accession>
    <name>A0A9L0ITU4_EQUAS</name>
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        <submittedName>
            <fullName evidence="7">Transcription elongation factor A N-terminal and central domain containing 2</fullName>
        </submittedName>
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        <name evidence="7" type="primary">TCEANC2</name>
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                <person name="Guo"/>
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                <person name="Zhao"/>
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                <person name="Yin"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
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        <property type="entry name" value="TFIIS_N"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11477">
        <property type="entry name" value="TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00509">
        <property type="entry name" value="TFS2N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF46942">
        <property type="entry name" value="Elongation factor TFIIS domain 2"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="40"/>
            <end position="114"/>
        </location>
    </feature>
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        <location>
            <begin position="131"/>
            <end position="208"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="208" mass="24376" checksum="21052FF81C52F63C" modified="2023-09-13" version="1">MDKFVIRTPRIQNSPQKKDPGGKIYKQATIESLKRVVVIEDIKRWKTMLELPDQSKENLVEALRELKKKIPSREVLKSTRIGHTVNKMRRHSDSEVACLAREVYTEWRTFIEKHLDRPSIEVRSDPKTETFRKKAQKLLSEALELEMDHLLVENIERETFHLCSRLINGPYRQTVRALVFTLKHRAEIRAQVKDGSLPVGTFVQTHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IUE4</accession>
    <name>A0A9L0IUE4_EQUAS</name>
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        <recommendedName>
            <fullName evidence="1">RNA-directed DNA polymerase</fullName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="J."/>
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                <person name="X."/>
                <person name="Zhao"/>
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                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
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        <text evidence="9">Component of the outer dynein arm-docking complex (ODA-DC) that mediates outer dynein arms (ODA) binding onto the doublet microtubule. Plays an essential role for the assembly of ODA-DC and for the docking of ODA in ciliary axoneme.</text>
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                <person name="Wang"/>
                <person name="J."/>
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                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
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                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
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                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
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                <person name="D.D."/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="3">
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        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
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        <property type="term" value="C:external side of plasma membrane"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:T cell activation"/>
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        <property type="entry name" value="Ig_SLAM-like_N"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="SLAM family member 7"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Ig_V-set"/>
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        <property type="entry name" value="SIGNALING LYMPHOCYTIC ACTIVATION MOLECULE"/>
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    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
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    <feature type="chain" id="PRO_5040278339" evidence="13">
        <location>
            <begin position="22"/>
            <end position="326"/>
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            <begin position="225"/>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="326" mass="36282" checksum="0C0CDAF21793AA5F" modified="2023-09-13" version="1">MAQHRLGILLLCLQTCLEVSGSNTDIITVNGILGESVTFPLNIQGSQQVNSIAWTSKSSVASIIPGNSGTPPIITVTHQDYYERINVSGQNYNLEIRNLRMEDAGTYKADVSLKTDHTTTKHFNLQVYRRLGKPKITQSLMTSMNNTCNVTLTCSVEKEEKNVTYTWSPLGEEGHVLQIFRTPVNQELTYTCTVWNPVSNNSDSISARQLCTDVAMGLHTRRTGLLSGLAVLSLFMLILPAVLLFLFCKRRQDAVSKNTIYTYITASRNTQQAESRIYDEIPQSKVFPAKEEPVNTIYSVVQCSDKEKISTQDSKPPGTSSYEIVI</sequence>
</entry>
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    <accession>A0A9L0IXV4</accession>
    <name>A0A9L0IXV4_EQUAS</name>
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            <fullName evidence="2">BRO1 domain-containing protein BROX</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">BRO1 domain- and CAAX motif-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">BROX</name>
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    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BROX family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IXV4"/>
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    </dbReference>
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        <property type="component" value="Chromosome 30"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="alix/aip1 like domains"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR004328">
        <property type="entry name" value="BRO1_dom"/>
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    <dbReference type="InterPro" id="IPR038499">
        <property type="entry name" value="BRO1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038898">
        <property type="entry name" value="BROX"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23032">
        <property type="entry name" value="BRO1 DOMAIN-CONTAINING PROTEIN BROX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23032:SF13">
        <property type="entry name" value="BRO1 DOMAIN-CONTAINING PROTEIN BROX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03097">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01041">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51180">
        <property type="entry name" value="BRO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRO1" evidence="6">
        <location>
            <begin position="99"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="343"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="235"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="343"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00017773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005044953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="379" mass="42579" checksum="49C081931745C15C" modified="2023-09-13" version="1">MTHWFHRNPLKATAPVSFNYYGVASGPAASKICSDLRSSRARLLELFTDLSCNPEMMKNAADSYFSLLQGFINSLDESTQESKLRYIQNFKWTDTLQGQVPSVTEDEAKEVHRSLKIAAGIFKHLKESHIPKLITPAEKGRDLEARLVDAYIIQCQAEAQEVTIARAIELKHAPGLIAALAYETANFYQKADHTLSSLEPAYSAKWRKYLLLKMCFYTAYAYCYHGQTLLASDKCGEAIRSLQEAEKLYAKAEALCKEYGETKGPGPTVKPSGHLFFRKLGNLVKNTLEKCQRENGFIYFQKVPTEAPQLELKANYGLVEPVPFEFPPTSAHWTPETLAAFDLTKRPKEDSTKPKPEEVVKPVKEPDIKPQKDTGCSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IY68</accession>
    <name>A0A9L0IY68_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ankyrin repeat domain 60</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ANKRD60</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Zhang"/>
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                <person name="Yin"/>
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        <text evidence="1 12">Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.</text>
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            <text>6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO2 + NADPH</text>
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    <comment type="pathway">
        <text evidence="2 12 15">Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 12">Homodimer.</text>
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        <text evidence="3 12 15">Belongs to the 6-phosphogluconate dehydrogenase family.</text>
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        <property type="project" value="UniProtKB-KW"/>
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    <feature type="active site" description="Proton donor" evidence="13">
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    <sequence length="470" mass="51977" checksum="958FDFE0763FD156" modified="2023-09-13" version="1">MGQNLILNMNDHGFVVCAFNRTVSKVDDFLANEAKGTKVVGAHSLEEMVSKLKKPRRIILLVKAGQAVDDFIEKLVPLLDTGDIIIDGGNSEYRDTTRRCRDLKAKGILFVGSGVSGGEEGARHGPSLMPGGNKEAWPHIKMIFQGIAAKVGTGEPCCDWVGSEGAGHFVKMVHNGIEYGDMQLICEAYHLMKDVLGMEHNEMAKAFEEWNKTELDSFLIEITANILKFQDTDGKYLLPKIRDSAGQKGTGKWTAISALEYGVPVTLIGEAVFARCLSSLKDERIQASGKLKGPRKIQFEGDKKSFLEDIRKALYASKIISYAQGFMLLRQAATEFGWTLNYGGIALMWRGGCIIRSVFLGKIKDAFDRNPELENLLLDDFFRSAVESCQDSWRRAVSTGVQMGIPMPCFTTALSFYDGYRHEMLPANLIQAQRDYFGAHTYELLAKPGQFIHTNWTGHGGSVSSSSYNA</sequence>
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                <person name="Guo"/>
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                <person name="S."/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="Xu"/>
                <person name="J."/>
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                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="32716" checksum="EC1A56BCBF3893F9" modified="2023-09-13" version="1">MNSSECIFRGGWELTCFVYPAKGLMQSRALIKMYRMDGRGAGCSPLHHRAVGGLGEPSPTWEAHHCPEEKMFGFHKPKMYRSIEGCCICRAKSSSSRFTDSKRYEKDFQSCFGLHETRSGDICNACVLLVKRWKKLPAGSKKNWNHVVDARAGPSLKTTLKPKKVKTLSGNRIKSNQISKLQKEFKRHNSDAHSTTSSASPAQSPCYSNQSDDGSDTEMASGSNRTPVFSFLDLTYWKRQKICCGIIYKGRFGEVLIDTHLFKPCCSNKKAAAEKPEEQRPEPLPLPPQEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J1M4</accession>
    <name>A0A9L0J1M4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0J1M4"/>
    <dbReference type="Ensembl" id="ENSEAST00005048296.1">
        <property type="protein sequence ID" value="ENSEASP00005043195.1"/>
        <property type="gene ID" value="ENSEASG00005035168.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164183"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd17958">
        <property type="entry name" value="DEADc_DDX43_DDX53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 6" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="21"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="224"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005043195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="358" mass="40252" checksum="BBBD492413839568" modified="2023-09-13" version="1">RDVRASRDGFQRPTPIQSQAWPIVLQGMDLIGVAQTGTGKTLSYLIPGFIHLDSQPISREERNGPGMLVLTPTRELALQVEAECSKYSYKGLKSVCVYGGGNRKEQIQHITKGVDIIIATPGRLNDLQMNKCVNLRSITYLVLDEADKMLDLGFEGQITKILLDVRPDRQTVMTSATWPHTIRRLARSYLKEPMIVYVGTLDLVAVNTVKQDIIVTTEEEKRTLIQEFLRNLSPEDKAIIFVSRKLVADDLSSDLSIQGVPVQSLHGSREQFDREQALDDFRSGRVKILIATDLAARGLDVRDVTHVYNYDFPKNLEEYVHRVGLRYSCKLCHQPLVKKFWGGDLPSVGLTSFTNKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J223</accession>
    <name>A0A9L0J223_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fibronectin type III domain-containing protein 3B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Factor for adipocyte differentiation 104</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">HCV NS5A-binding protein 37</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">FNDC3B</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the FNDC3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J223"/>
    <dbReference type="Ensembl" id="ENSEAST00005053853.1">
        <property type="protein sequence ID" value="ENSEASP00005044057.1"/>
        <property type="gene ID" value="ENSEASG00005017198.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157005"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000175">
        <property type="entry name" value="Fibronectin type III domain containing 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000180">
        <property type="entry name" value="Fibronectin type III domain containing 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000185">
        <property type="entry name" value="Fibronectin type III domain containing 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000195">
        <property type="entry name" value="Fibronectin type III domain containing 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000210">
        <property type="entry name" value="Fibronectin type III domain containing 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000309">
        <property type="entry name" value="Fibronectin type III domain containing 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000396">
        <property type="entry name" value="Fibronectin type III domain containing 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000603">
        <property type="entry name" value="Fibronectin type III domain containing 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000337">
        <property type="entry name" value="fibronectin type-III domain-containing protein 3A isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050617">
        <property type="entry name" value="E3_ligase_FN3/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIM36-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24099:SF19">
        <property type="entry name" value="FIBRONECTIN TYPE III DOMAIN-CONTAINING PROTEIN 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
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            <begin position="1181"/>
            <end position="1204"/>
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    <sequence length="1206" mass="132849" checksum="4E8F3C37125F4647" modified="2023-09-13" version="1">MYVTMMMTDQIPLELPPLLNGEVAMMPHLVNGDAAQQVILVQVNPGETFTIRAEDGTLQCIQGPAEVPMMSPNGSIPPIHVPPGYISQVIEDNTGVRRVVVTPQSPECYPPSYPSAMSPTHHLPPYLTHHPHFIHNSHTAYYPPVTGPGDMPPQFFPQHHLPPTIYGEQEIIPLYGMSSYITREDQYSKPPHKKLKDRQIDRQNRLNSPPSSIYKSSCTTVYNGYGKGHSGGGGGGGSGGGPGIKKTERRARSSPKSNDSDLQEYELEVKRVQDILSGIEKPQVSNIQARAVVLSWAPPVGLSCGPHSGLSFPYSYEVALSDKGRDGKYKIIYSGEELECNLKDLRPATDYHVRVYAMYNSVKGSCSEPVSFTTHSCAPECPFPPKLAHRSKSSLTLQWKAPIDNGSKITNYLLEWDEGKRNSGFRQCFFGSQKHCKLTKLCPAMGYTFRLAARNDIGTSGYSQEVVCYTLGNIPQMPSAPRLVRAGVTWVTLQWNKPEGCSPEEVVTYTLEIQEDENDNLFHPKYTGEDLTYTVKNLKRSTQYKFRLTASNMEGKSCPSEVLVCTTSPDRPGPPTRPLIKGPVTSHGFSVKWDPPKDNGGSEILKYLLEITDGNSEGEASQWEVAYSGSATEYTLTHLKPGTLYKLRACCISTGGHSQCSESLPVRTLSIAPGQCRPPRVLGRPKHKEVHLEWDVPASESGCEVSEYSVEMTEPEDVASEVYHGPELECTVGNLLPGTVYRFRVRALNDGGYGPYSDVSEITTAAGPPGQCKAPCISFTPDGCVLVSWESPESAGADISEYRLEWGEDEESLELVYHGADTGFEMRDLLPAAQYCCRLQAVNQAGAGPYSELVLCQTPASAPDPVSTLCVLEEETLNAYPDSPSLCLVLNWEEPCNNGSEILAYNIDLGDTSITVGNTTSHIMKDLLPETTYRIRIQAINEIGAGPFSQFIKAKTRPLPPLPPRLECAAAGPQSLKLKWGDSNAKAHAADDMVYTLQLEDRNKRFISIYRGPSHTYKVQRLTEFTCYSFRIQAASEAGEGPFSETYTFSTTKSVPPAIKAPRVTQLEGNSCEILWETVPPMKGDPVSYILQVLVGRESEYKQVYKGEEATFQISGLQTNTDYRFRVCACRRCLDTSQELSGAFSPSAAFVLQRSEVMLPGDMGSLDDPKMKSMMPTDEQFAALIVVGFATLSIFFAFILQYFLMK</sequence>
</entry>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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    <accession>A0A9L0J339</accession>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Li"/>
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                <person name="Liu"/>
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                <person name="G."/>
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                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J339"/>
    <dbReference type="Ensembl" id="ENSEAST00005064683.1">
        <property type="protein sequence ID" value="ENSEASP00005047686.1"/>
        <property type="gene ID" value="ENSEASG00005027017.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159907"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 11"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001405">
        <property type="term" value="C:PAM complex, Tim23 associated import motor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000001">
        <property type="entry name" value="Import inner membrane translocase subunit tim14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12763:SF7">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY C MEMBER 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0811">Translocation</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="95"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005047686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="149" mass="15956" checksum="3B993108B2CADFCF" modified="2023-09-13" version="1">MAARGGVASAGEGLRYAEYSPPSTRRPDADIDQGLARSLIAVGLGVAALAFAGRYAFQIWKPLEQVITDAAKKISAPSLSSYYKGGFEQKMSRREASLILGVSLSAGKAKIRTAHRRIMILNHPDKGGSPYLATKINEAKDLLEATTKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J5M2</accession>
    <name>A0A9L0J5M2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">E3 ubiquitin-protein ligase RNF183</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">RNF183</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Acts as an E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Triggers apoptosis in response to prolonged ER stress by mediating the polyubiquitination and subsequent proteasomal degradation of BCL2L1. May collaborate with FATE1 to restrain BIK protein levels thus regulating apoptotic signaling.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Interacts with FATE1. Interacts with SEC16A. Interacts with BCL2L1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Golgi apparatus</location>
            <location evidence="19">cis-Golgi network membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lysosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0J5M2"/>
    <dbReference type="Ensembl" id="ENSEAST00005040251.1">
        <property type="protein sequence ID" value="ENSEASP00005047673.1"/>
        <property type="gene ID" value="ENSEASG00005030045.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106822723"/>
    <dbReference type="KEGG" id="eai:106822723"/>
    <dbReference type="CTD" id="138065"/>
    <dbReference type="GeneTree" id="ENSGT00940000162965"/>
    <dbReference type="OMA" id="PNHIILE"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033106">
        <property type="term" value="C:cis-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902237">
        <property type="term" value="P:positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd16556">
        <property type="entry name" value="RING-HC_RNF183-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000409">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase RNF183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051435">
        <property type="entry name" value="RING_finger_E3_ubiq-ligases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027370">
        <property type="entry name" value="Znf-RING_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22791:SF7">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RNF183"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22791">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13445">
        <property type="entry name" value="zf-RING_UBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="16" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="18" id="KW-0458">Lysosome</keyword>
    <keyword evidence="17" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="10 23" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="24">
        <location>
            <begin position="13"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00053921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00064632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00067422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005047673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="192" mass="21800" checksum="25E2600F1A7E012D" modified="2023-09-13" version="1">MAEQQGREPEAECPVCWNPFNNTFHTPKVLDCCHSFCLECLAHLSLVTPARRQLLCPLCRQPTVLASGQPVTDLPTDIAMLTLLRLEPHHVILEGRQLCLKDQPKSRYFLRQPQVYTLDLGPEPVNQTGPPQDMASATRPMPISIPSHYSLRECSRNPQFRIFAYLMAVILSVTLLLIFSIFWSKQFLWGVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JAT6</accession>
    <name>A0A9L0JAT6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Prefoldin subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">PFDN4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3 6">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits. Interacts with URI1; the interaction is phosphorylation-dependent and occurs in a growth-dependent manner.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JAT6"/>
    <dbReference type="Ensembl" id="ENSEAST00005076274.1">
        <property type="protein sequence ID" value="ENSEASP00005049483.1"/>
        <property type="gene ID" value="ENSEASG00005023279.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006696"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23165">
        <property type="entry name" value="Prefoldin_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000005">
        <property type="entry name" value="Prefoldin subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016661">
        <property type="entry name" value="PFDN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21100">
        <property type="entry name" value="PREFOLDIN SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21100:SF9">
        <property type="entry name" value="PREFOLDIN SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016477">
        <property type="entry name" value="Prefoldin_subunit_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="42"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00062093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR016477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005049483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="143" mass="16463" checksum="C78A84D41397355D" modified="2023-09-13" version="1">MKFLLPCPHSIAELMLWAAEDVNVTFEDQQKINKFARNTSRITELKEEIEVKKKQLQNLEDACEDIMLADDDCLMIPYQIGDVFISHSQEETQEMLEEAKKNLQEEIAALESRVESIQRVLADLKVQLYAKFGSNINLEADES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JB61</accession>
    <name>A0A9L0JB61_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">U11/U12 small nuclear ribonucleoprotein 35 kDa protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">U1 snRNP-binding protein homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SNRNP35</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="5">Component of the U11/U12 snRNPs that are part of the U12-type spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014714290.1">
        <property type="nucleotide sequence ID" value="XM_014858804.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014714291.1">
        <property type="nucleotide sequence ID" value="XM_014858805.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014714292.1">
        <property type="nucleotide sequence ID" value="XM_014858806.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JB61"/>
    <dbReference type="SMR" id="A0A9L0JB61"/>
    <dbReference type="Ensembl" id="ENSEAST00005037601.1">
        <property type="protein sequence ID" value="ENSEASP00005059571.1"/>
        <property type="gene ID" value="ENSEASG00005024491.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005044820.1">
        <property type="protein sequence ID" value="ENSEASP00005036687.1"/>
        <property type="gene ID" value="ENSEASG00005024491.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005047287.1">
        <property type="protein sequence ID" value="ENSEASP00005038723.1"/>
        <property type="gene ID" value="ENSEASG00005024491.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005059571.1">
        <property type="protein sequence ID" value="ENSEASP00005055941.1"/>
        <property type="gene ID" value="ENSEASG00005024491.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005059994.1">
        <property type="protein sequence ID" value="ENSEASP00005049603.1"/>
        <property type="gene ID" value="ENSEASG00005024491.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106842335"/>
    <dbReference type="KEGG" id="eai:106842335"/>
    <dbReference type="CTD" id="11066"/>
    <dbReference type="GeneTree" id="ENSGT00940000157648"/>
    <dbReference type="OMA" id="FERSRVM"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005689">
        <property type="term" value="C:U12-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017069">
        <property type="term" value="F:snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd12237">
        <property type="entry name" value="RRM_snRNP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000132">
        <property type="entry name" value="Small nuclear ribonucleoprotein U11/U12 subunit 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034146">
        <property type="entry name" value="snRNP35_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051183">
        <property type="entry name" value="U1_U11-U12_snRNP_70-35kDa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN 70 KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13952:SF6">
        <property type="entry name" value="U11_U12 SMALL NUCLEAR RIBONUCLEOPROTEIN 35 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="8">
        <location>
            <begin position="51"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="146"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="146"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="174"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="192"/>
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                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Zhou"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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            <authorList>
                <consortium name="Ensembl"/>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
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        <source>
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    <sequence length="318" mass="34799" checksum="DF00C7F4937F8063" modified="2023-09-13" version="1">MWLPLLLGTLLWVALWLLRDQQSLPASDAFVFITGCDSGFGRLLALRLDQRGFRVLASCLTPSGAEDLQRVASSRLHATLLDVTDPQSVQRAAKWVETHVGEAGLFGLVNNAGVAGIIGPTPWLTRDDFHRVLNVNTLGPIGVTLALLPLLQQARGRVINVTSVLGRLAANGGGYCVSKFGLEAFSDSLRRDVASFGVRVSIVEPGFFQTPVTNLKSLENALQACWARLPPATQAHYGEAFLTKYLKVQQRIMNLLCDPDLTKVSKCLEHALTARHPRTRYSPGWDAKLLWLPASYLPASLVDAMLTWVLPKPAHAIY</sequence>
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    <name>A0A9L0JKX0_EQUAS</name>
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        <recommendedName>
            <fullName evidence="23">Toll-like receptor 3</fullName>
        </recommendedName>
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    <gene>
        <name evidence="27" type="primary">TLR3</name>
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    <organism evidence="27 28">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <location evidence="2">Early endosome</location>
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            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
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        <text evidence="4">Belongs to the Toll-like receptor family.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="LEURICHRPT"/>
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    <keyword evidence="13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="21" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0391">Immunity</keyword>
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    <keyword evidence="7" id="KW-0399">Innate immunity</keyword>
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    <keyword evidence="8" id="KW-0433">Leucine-rich repeat</keyword>
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    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="10 25" id="KW-0732">Signal</keyword>
    <keyword evidence="9 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
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        <location>
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            <end position="26"/>
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            <end position="908"/>
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    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="708"/>
            <end position="729"/>
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    <feature type="domain" description="TIR" evidence="26">
        <location>
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            <end position="901"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
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            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00072834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005053013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="908" mass="104337" checksum="357D8428F2ADADF8" modified="2023-09-13" version="1">MDRIMSQSLPYHIYSFLRLLPFWVLCASCNKCAVRHEVADCSHLKLTQIPDDLPANITVLNLTHNQFRRLPPDNFTRYSQLTILDGGFNCISKLEPELCQKLPLLEILDLQHNELSQLSDKTFMFCMNLTELHLRSNSIQKIQNNPFKNLKNLIKLDLSHNGLSSTKLGTQLQLENLQELLLSNNKIQALRRDELDFLGNSSLKKLELSSNQIKEFSPGCFQAIGKLFGLSLNNVQLGPSLTEKLCLELSNTSIQNLSLSNTQLYRTSNTTFVGLKQTNLTMLDLSHNNLNVIGNDSFAWLPHLEYFFLEYNNIEHLYAHSFYGLFNVRYLNLRRSFTKQSISLASLPKIDDSSFQWLKCLEYLNMEDNNFPGIKSDMFTGLVKLKHLSLSNSFSSLRTLTNETFLSLAHSPLLTLNLTKNKISKLESGAFSWLGHLKILDLGLNEIGQELTGQEWRGLENIFEIYLSYNKYLQLTSNSFALVPSLQRLMLRRVALKNVDSSPPPFRPLHNLTILDLSNNNIANINDELLEGLEKLEILDLQHNNLARLWKHANPGGPVHFLKGLSHLHVLNLESNGFDEIPAEAFKNLFELKSINLGLNNLNILPPSVFDDQVSLKSLSLQKNLITSVEKNVFGPALKNLSSLDMSFNPFDCTCESIAWFVNWINGTHTNISELSSHYLCNTPPQYHGFPVMLFDTSSCKDSAPFELLFMINTSFLLIFIFIVLLIHFEGWRISFYWNVSVHRVLGFKEIDSQPEQFEYAAYIIHAYKDRDWVWEHFSPMEEQDQTLKFCLEERDFQAGVLELEAIINSIKRSRKIIFIITQHLLKDPLCKRFKVHHAVQQAIEQNLDSIILIFLEEIPDYKLNHALCLRRGMFKSHCILNWPVQKERINAFHHKLQVALGSRNSAH</sequence>
</entry>
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    <accession>A0A9L0JL44</accession>
    <name>A0A9L0JL44_EQUAS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <property type="component" value="Chromosome 2"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="51" mass="5673" checksum="A729AB64BED8D96C" modified="2023-09-13" version="1">MRHHISSHFLGHQSHCPVSWMPSLVCLLVGDCEGPRMQCSWPKGPLLATGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JN55</accession>
    <name>A0A9L0JN55_EQUAS</name>
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            <fullName evidence="22">Aspartyl/asparaginyl beta-hydroxylase</fullName>
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            <fullName evidence="23">Aspartate beta-hydroxylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Peptide-aspartate beta-dioxygenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">ASPH</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
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                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Specifically hydroxylates an Asp or Asn residue in certain epidermal growth factor-like (EGF) domains of a number of proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-aspartyl-[protein] + 2-oxoglutarate + O2 = 3-hydroxy-L-aspartyl-[protein] + succinate + CO2</text>
            <dbReference type="Rhea" id="RHEA:11508"/>
            <dbReference type="Rhea" id="RHEA-COMP:9867"/>
            <dbReference type="Rhea" id="RHEA-COMP:14951"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17427"/>
            <dbReference type="ChEBI" id="CHEBI:29961"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="EC" id="1.14.11.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aspartyl/asparaginyl beta-hydroxylase family.</text>
    </comment>
    <dbReference type="EC" id="1.14.11.16" evidence="21"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0JN55"/>
    <dbReference type="Ensembl" id="ENSEAST00005066558.1">
        <property type="protein sequence ID" value="ENSEASP00005050585.1"/>
        <property type="gene ID" value="ENSEASG00005029180.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156304"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062101">
        <property type="term" value="F:peptidyl-aspartic acid 3-dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045862">
        <property type="term" value="P:positive regulation of proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000151">
        <property type="entry name" value="Aspartyl/asparaginyl beta-hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000154">
        <property type="entry name" value="Aspartyl/asparaginyl beta-hydroxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.330:FF:000004">
        <property type="entry name" value="aspartyl/asparaginyl beta-hydroxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007943">
        <property type="entry name" value="Asp-B-hydro/Triadin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007803">
        <property type="entry name" value="Asp/Arg/Pro-Hydrxlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039038">
        <property type="entry name" value="ASPH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12366">
        <property type="entry name" value="ASPARTYL/ASPARAGINYL BETA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12366:SF33">
        <property type="entry name" value="ASPARTYL_ASPARAGINYL BETA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05279">
        <property type="entry name" value="Asp-B-Hydro_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05118">
        <property type="entry name" value="Asp_Arg_Hydrox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JNH4</accession>
    <name>A0A9L0JNH4_EQUAS</name>
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        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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    <comment type="subcellular location">
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            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="component" value="Chromosome 20"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="7tmA_OR5P-like"/>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="entry name" value="Olfact_rcpt"/>
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    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="64"/>
            <end position="89"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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    <feature type="transmembrane region" description="Helical" evidence="13">
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    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="44"/>
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    </feature>
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            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005054774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="314" mass="35118" checksum="D7674DBDC5A0CA19" modified="2023-09-13" version="1">MDALGDRNCTEVTGFILLGLTDDPILRVILFMIILCIYLVTISGNLSTIILIRISSQLHHPMYFFLSHLALADIGYSSSVTPNMLINFLVEKNTISYFGCATQLGSVAFFGATECFLLAAMAYDRFIAICSPLLYATKMSTQVCVQLLVLAYIGGFYNAFSFTICVYCLLFCGPNRVNHFFCDFTPLVELSCSDMSIPAVVPSYMVGFIIVATVFVIAVSYIYILITILKMRSTEGCRKAFSTCASHLTAVTLYYGTITFIYVLPKSYYTTDQNKVVSVFYMVVIPMLNPLIYSLRNNEIKGALKREFVRKIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JRM1</accession>
    <name>A0A9L0JRM1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Sphingosine-1-phosphate phosphatase 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">SGPP2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>sphing-4-enine 1-phosphate + H2O = sphing-4-enine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27518"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57756"/>
            <dbReference type="ChEBI" id="CHEBI:60119"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:27519"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>sphinganine 1-phosphate + H2O = sphinganine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:27514"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57817"/>
            <dbReference type="ChEBI" id="CHEBI:57939"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:27515"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the type 2 lipid phosphate phosphatase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014698739.1">
        <property type="nucleotide sequence ID" value="XM_014843253.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JRM1"/>
    <dbReference type="Ensembl" id="ENSEAST00005074958.1">
        <property type="protein sequence ID" value="ENSEASP00005052637.1"/>
        <property type="gene ID" value="ENSEASG00005033479.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106832476"/>
    <dbReference type="KEGG" id="eai:106832476"/>
    <dbReference type="CTD" id="130367"/>
    <dbReference type="GeneTree" id="ENSGT00940000159500"/>
    <dbReference type="OMA" id="KFMVGIV"/>
    <dbReference type="OrthoDB" id="301434at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042392">
        <property type="term" value="F:sphingosine-1-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061469">
        <property type="term" value="P:regulation of type B pancreatic cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006670">
        <property type="term" value="P:sphingosine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03388">
        <property type="entry name" value="PAP2_SPPase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.144.10:FF:000011">
        <property type="entry name" value="sphingosine-1-phosphate phosphatase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.144.10">
        <property type="entry name" value="Phosphatidic acid phosphatase type 2/haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000326">
        <property type="entry name" value="PAP2/HPO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036938">
        <property type="entry name" value="PAP2/HPO_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969:SF14">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14969">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01569">
        <property type="entry name" value="PAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00014">
        <property type="entry name" value="acidPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48317">
        <property type="entry name" value="Acid phosphatase/Vanadium-dependent haloperoxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="80"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="114"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="152"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="207"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="270"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="312"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="367"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphatidic acid phosphatase type 2/haloperoxidase" evidence="12">
        <location>
            <begin position="111"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="391" mass="44024" checksum="EB53BC1CE2AFC68A" modified="2023-09-13" version="1">MAELLRSLQDSQLVARFQRRCGLFPAPDEGPGEDGAAPGLGHLPGATGKGRGGPAHGLRRVTASQAHVQKYIVKNYFYYYLFRFSAALGQEVFYITFLPFTYWNVDSYVSRRLIIIWVLVMYVGQVAKDILKWPRPFSPPVVKLEKRLIAEYGMPSTHAMAATAIPFTVLISTVDRYQYPFVWGLIVAVVFSTLVCLSRLYTGMHTVLDVLGGILITMILIVLTYPAWTLIDHLDSASPLFPVCVIVVPFFLCYNYPVSEYYSPTRADTTTILAAGAGVIIGFWINHFFQLVSEPIESFPVIQNIPPLTTDMLVLGLTKFTVGIVLIFLVRQLVQNLSLQVLYSWFKVVTRNKEARRRLEIEVPYKFVTYTSVGICATTFVPMLHRFLGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JSX7</accession>
    <name>A0A9L0JSX7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Zinc finger and BTB domain-containing protein 18</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Zinc finger protein 238</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ZBTB18</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Interacts with DNMT3A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the krueppel C2H2-type zinc-finger protein family. ZBTB18 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014723154.1">
        <property type="nucleotide sequence ID" value="XM_014867668.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JSX7"/>
    <dbReference type="Ensembl" id="ENSEAST00005054028.1">
        <property type="protein sequence ID" value="ENSEASP00005052260.1"/>
        <property type="gene ID" value="ENSEASG00005030332.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106848038"/>
    <dbReference type="KEGG" id="eai:106848038"/>
    <dbReference type="CTD" id="10472"/>
    <dbReference type="GeneTree" id="ENSGT00940000155092"/>
    <dbReference type="OrthoDB" id="4748970at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000792">
        <property type="term" value="C:heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd18324">
        <property type="entry name" value="BTB_POZ_ZBTB18_RP58"/>
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        <property type="entry name" value="Zinc finger and BTB domain-containing protein 18"/>
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        <property type="entry name" value="Zinc finger and BTB domain-containing protein 18"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger and BTB domain-containing protein 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000892">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 3"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF18">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13894">
        <property type="entry name" value="zf-C2H2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 15" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="33"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="379"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="419"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="447"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="475"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="130"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038422"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066152"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00070991"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00079234"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
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    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052260.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="531" mass="59480" checksum="7EBACC709DD4070F" modified="2023-09-13" version="1">MCPKGYEDSMEFPDHSRHLLQCLREQRHQGFLCDCTVLVGDAQFRAHRAVLASCSMYFHLFYKDQLDKRDIVHLNSDIVTAPAFALLLEFMYEGKLQFKDLPIEDVLAAASYLHMYDIVKVCKKKLKEKATTEADSTKKEEDASSCSDKVESLSDGSSHMAGDLPSDEDEGDDEKLNILPSKRDLAAEPGNMWMRLPSDSAGIPQAGGEAEPHATAAGKTVASPCSSTESLSQRSVTSVRDSADVDCVLDLSVKSSLSGVENLNSSYFSSQDVLRSNLVQVKVEKEASCDESDVGTNDYDMEHSTVKESVSTNNRVQYEPAHLAPLREDSVLRELEREDKASDDEMMTPENERVQVEGSMESSLLPYVSNILSPAGQIFMCPLCNKVFPSPHILQIHLSTHFREQDGLRSKPAADVNVPTCSLCGKTFSCMYTLKRHERTHSGEKPYTCTQCGKSFQYSHNLSRHAVVHTREKPHACKWCERRFTQSGDLYRHIRKFHCELVNSLSVKSEALSLPAVRDWTLEDSSQELWK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JT86</accession>
    <name>A0A9L0JT86_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">DMRT like family A1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">DMRTA1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Probable transcription factor that plays a role in configuring the spinal circuits controlling stride in vertebrates. Involved in neuronal specification within a specific subdivision of spinal cord neurons and in the development of a coordinated locomotor network controlling limb movements. May regulate transcription during sexual development.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DMRT family.</text>
    </comment>
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    <dbReference type="Ensembl" id="ENSEAST00005075265.1">
        <property type="protein sequence ID" value="ENSEASP00005052370.1"/>
        <property type="gene ID" value="ENSEASG00005036106.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160640"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008344">
        <property type="term" value="P:adult locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060179">
        <property type="term" value="P:male mating behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001541">
        <property type="term" value="P:ovarian follicle development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14417">
        <property type="entry name" value="CUE_DMA_DMRTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1040.10:FF:000001">
        <property type="entry name" value="doublesex- and mab-3-related transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1040.10">
        <property type="entry name" value="DM DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001275">
        <property type="entry name" value="DM_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036407">
        <property type="entry name" value="DM_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005173">
        <property type="entry name" value="DMA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026607">
        <property type="entry name" value="DMRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046472">
        <property type="entry name" value="DMRT5_1_DMB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322">
        <property type="entry name" value="DOUBLESEX AND MAB-3 RELATED TRANSCRIPTION FACTOR DMRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12322:SF71">
        <property type="entry name" value="DOUBLESEX- AND MAB-3-RELATED TRANSCRIPTION FACTOR A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00751">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03474">
        <property type="entry name" value="DMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20624">
        <property type="entry name" value="DMRT5_DMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00301">
        <property type="entry name" value="DM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82927">
        <property type="entry name" value="Cysteine-rich DNA binding domain, (DM domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS40000">
        <property type="entry name" value="DM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50809">
        <property type="entry name" value="DM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="8 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0726">Sexual differentiation</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DM" evidence="14">
        <location>
            <begin position="91"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="DM" evidence="12">
        <location>
            <begin position="91"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="147"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="210"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="210"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="222"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="263"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="280"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
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                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Plays a critical role in G2/M checkpoint and nuclear divisions. A key player in the DNA damage-activated ATR/ATM signaling cascade since it is required for the proper phosphorylation of H2AX, RPA, CHEK2 and CHEK1. Plays a critical role in chromosome segregation, acting as a mediator required for the maintenance of genomic stability through modulation of MDC1, RPA and CHEK1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts (via N-terminus) with ATRIP. Interacts with ATM, ATR and MDC1. Interacts with XPA (via N-terminus) upon UV irradiation. Interacts with CEP83, CCDC92, TTBK2, DVL3, NPHP3 and weakly with NPHP4. Interacts with DZIP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005061154.1">
        <property type="protein sequence ID" value="ENSEASP00005054562.1"/>
        <property type="gene ID" value="ENSEASG00005005271.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183078"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0097539">
        <property type="term" value="C:ciliary transition fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060271">
        <property type="term" value="P:cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1470.10:FF:000001">
        <property type="entry name" value="Centrosomal protein of 164 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1470.10">
        <property type="entry name" value="Photosystem I PsaD, reaction center subunit II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051841">
        <property type="entry name" value="MT-Golgi_org_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18902:SF27">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 164 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18902">
        <property type="entry name" value="NUCLEAR MITOTIC APPARATUS PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="8" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="9 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="19">
        <location>
            <begin position="56"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="103"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="226"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="369"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="812"/>
            <end position="967"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1000"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1182"/>
            <end position="1234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1254"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1321"/>
            <end position="1363"/>
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    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1384"/>
            <end position="1403"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1769"/>
            <end position="1789"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="1510"/>
            <end position="1593"/>
        </location>
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    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="1696"/>
            <end position="1747"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
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            <begin position="280"/>
            <end position="298"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="18">
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            <begin position="314"/>
            <end position="324"/>
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            <begin position="409"/>
            <end position="419"/>
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            <begin position="487"/>
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            <begin position="814"/>
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            <begin position="914"/>
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            <begin position="1001"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
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            <begin position="1080"/>
            <end position="1101"/>
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            <begin position="1195"/>
            <end position="1214"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1339"/>
            <end position="1350"/>
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    </feature>
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            <dbReference type="ARBA" id="ARBA00004114"/>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022776"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022794"/>
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            <dbReference type="ARBA" id="ARBA00023054"/>
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            <dbReference type="ARBA" id="ARBA00023204"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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            <dbReference type="ARBA" id="ARBA00023306"/>
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            <dbReference type="ARBA" id="ARBA00067900"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
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            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
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            <dbReference type="Ensembl" id="ENSEASP00005054562.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1993" mass="221887" checksum="215E9C21C7E5FC2B" modified="2023-09-13" version="1">MAGRPIRIGDQLVLEEDYDETYIPSEQEILEFAKEIGIDPIKEPELMWLAREGIVAPLPVEWKPCQDITGDIYYFNFANGQSMWDHPCDEHYRNLVIQERGKLSTSGATKKKEKKKKKEKKDKKDKETSRSPLETQPEQGLLPSSSFLRGPSPLPAPGLADMDLDQKMQARSEGSFKKGKSPCMLGDTPWPLMGTLPGKLQPLSKGPASRIHQIFADVEKILGRAPVQRRTELGDQQGLEKPQKPTEKIYLGFSDPEIEELEMRTRQQKPGAPGPENPRPLQSGQDVLESRSQASIHSKLSEAVKGPQLKGEQHSQNMAKLSSTGPGGDKGQSPIPLPCSEGEPSLSPCSSDHMLPIRKSKLFLLDSSPAEDLSWQGVYGEGGSVGRGRRRREPPGLWMGQVSNLVNKDTPRSCKKTEPSDAEAPGASAEDPPQGLFLVPPATLVSEPAQNTPSGSAPGESPTSEKRQPPGSPESPEEDRKSGGSGPDLESSSSLASPLGSQILGEVNNFPWDLQSSQGSERGEGPSGPWPRDPHSSPFLVPQLSHMQSSADEQSESEDYSEDQRFYQHILQMVKISRRLEGLGLPESMPERPSKDIASMVCCVAPESSRMSSEGEHEAIRAVERDSRFLAWRSELLEHPPEAALAPACQEASQQACFQPSSSPLRQGPVELSSNRGLTAEPGKMPLLSQAVGPSLAPVHTPLGGLAPLRGLVDAPPSALRGSQSVSLGSSVETGQLGELMLPSQGLKTSAYTKGLLGSIHEDTNALNLLALGEETNEEDEVESDNQSVRSSSDLLKNLHLDIGALVGDFEYEESPRTSQPEEKKDISLDSDAAGPPTPGKLFSQGADSSLSSADDKGRQGRGAHWLPGKEKNEKSDPRTSRSLVTPGVDPGGDQPAKADKKEAPEDMVDAGEEGSRKEEAAKEPKREASVLKESRSDGSEESEISEHVKEPQLSDSAASDPKSFLGLDFGFRSRISEHLLDVDVLSPVLDGACWEVQGLGREDKDDSQSSRDELKSKQSKGSERLSPPLLHGERLQSPLHSQATKDGPLQASEGQLERKGAEEPGEDSAGSTIPPVSPQREEIPSPPAAHERGKEQHSQAEEPEEKGVVSPTPPISPEVQSSEPVAPPTQLSEAALKTMQEAVAQELEQDQRQLLESKREKIQQLREKLWQEEEQEILQLQQQKEKALSSLKEQLQRATEEEETRMREEESRRLSRLRARVQSSTEAHEVQIRAEQEASLQRLREELESLQKAERASLEQRNRQMLEQLKEEMEASEKREQAALNAEKEKALQQLREQLEGERKEAVAALEKEHRAELERLSSSLEAKHRQVVSSLQKKMEEAQQKDEAQLQESLGRAEQRAHQKVHQVLEYEQELSGLLREKRQEVEREHERKLDKMKEEHRQVVAEAREQYEAEERKQRAELLGHLTGELERLRRAHERELETMRQDQDRQLEDLRRRHREQERKLQELEVELETRTKDVKATLTQLDIQETARKEKQQLLDVQRKVALESEEATATRQHLEEAKKEHTHLLESNRQLRRILDELQARKLELESQVDLLQTQSQRLQKRVSDLEAEAQRKRDTLKELAVEESNASARLEPDLHIEDLRKSLGTNQTKEVSSSLSQSKEEADLSLDSVRHYLSAEGIALCSAREFLVRQSRSMRRRQTALRAAQQHWRHELASAQEAAKDPPGTKALEDMRKDLEEETRHLDEMKSAMRKGHDLLKKKEEKLNQLEFSLREEASDEDTLRGTPAKKVVTFDLSDMEDMSSESSESCPLPHVTPTPGPASPNKIHYLSSSLQRISSQLNGVLSMLGSLSTQPPPPLFTSTPAQTASWSPRSIPIPTYPSRVSASFSATPTSTQWAWDPGLGPRLSSSVAQTVDDFLVEKWRKYFPTGVPFLSSGPPPLENRLGYVSASEQLRLLQRSSPRPQVPEAGSTNFQGMIDANRKWLERYKNDPKLHLFSSVPKPTAPLGLLQLGLDENNRLKLYHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JXM8</accession>
    <name>A0A9L0JXM8_EQUAS</name>
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        <submittedName>
            <fullName evidence="12">Zinc finger protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">ZNF3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014690866.1">
        <property type="nucleotide sequence ID" value="XM_014835380.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014690939.1">
        <property type="nucleotide sequence ID" value="XM_014835453.3"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_044747019.1"/>
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    <dbReference type="RefSeq" id="XP_044602955.1">
        <property type="nucleotide sequence ID" value="XM_044747020.1"/>
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    <dbReference type="RefSeq" id="XP_044602956.1">
        <property type="nucleotide sequence ID" value="XM_044747021.1"/>
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    <dbReference type="AlphaFoldDB" id="A0A9L0JXM8"/>
    <dbReference type="Ensembl" id="ENSEAST00005054690.1">
        <property type="protein sequence ID" value="ENSEASP00005054812.1"/>
        <property type="gene ID" value="ENSEASG00005024752.1"/>
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    <dbReference type="GeneID" id="106827476"/>
    <dbReference type="KEGG" id="eai:106827476"/>
    <dbReference type="CTD" id="7551"/>
    <dbReference type="GeneTree" id="ENSGT00940000153104"/>
    <dbReference type="OMA" id="MGDRPHK"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
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    <dbReference type="GO" id="GO:0005634">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
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        <property type="entry name" value="KRAB"/>
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    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
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    <dbReference type="InterPro" id="IPR050758">
        <property type="entry name" value="Znf_C2H2-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
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    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
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    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
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    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="11">
        <location>
            <begin position="52"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="201"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="285"/>
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    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="313"/>
            <end position="340"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="397"/>
            <end position="424"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="447" mass="50807" checksum="013645FB019A18FD" modified="2023-09-13" version="1">METQADRVSQEPQALLESALPSSKVPAFADKDSLGDEMLAAALLKAKSRELVTFEDVAVYFIRKEWKRLEPAQRDLYRDVMLENYGNVFSLDGETRTEHDQEISEDTGSHGVLLGRLQKDISQGFKFEEAYEQEVTLKGQLGNSSGERLNRKIQDFGQVIVEEKLSPRGERSEKYNDFGNSFTVSSNLVSHQRLPVGDRPHKCDECSKSFNRTSDLIQHQRIHTGEKPYECSECGKAFSQSSHLIQHQRIHTGEKPYECNDCGKTFSCSSALILHRRIHTGEKPYECSECGKTFSWSSTLTHHQRIHTGEKPYACNECGKAFSRSSTLIHHQRIHTGEKPYECNECGKAFSQSSHLYQHQRIHTGEKPYECMECGGKFTYSSGLIQHQRIHTGENPYECSECGKAFRYSSALVRHQRIHTGEKPLNVMGMSRSSLRVTAELNIREST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K383</accession>
    <name>A0A9L0K383_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Potassium voltage-gated channel subfamily A member 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">KCNA3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K383"/>
    <dbReference type="Ensembl" id="ENSEAST00005062521.1">
        <property type="protein sequence ID" value="ENSEASP00005055805.1"/>
        <property type="gene ID" value="ENSEASG00005032553.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160210"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008076">
        <property type="term" value="C:voltage-gated potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005251">
        <property type="term" value="F:delayed rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001508">
        <property type="term" value="P:action potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051260">
        <property type="term" value="P:protein homooligomerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000002">
        <property type="entry name" value="Potassium voltage-gated channel subfamily a member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000007">
        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000021">
        <property type="entry name" value="Potassium voltage-gated channel subfamily A member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.350">
        <property type="entry name" value="Voltage-gated potassium channels. Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005821">
        <property type="entry name" value="Ion_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003968">
        <property type="entry name" value="K_chnl_volt-dep_Kv"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003972">
        <property type="entry name" value="K_chnl_volt-dep_Kv1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004050">
        <property type="entry name" value="K_chnl_volt-dep_Kv1.3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003131">
        <property type="entry name" value="T1-type_BTB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028325">
        <property type="entry name" value="VG_K_chnl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027359">
        <property type="entry name" value="Volt_channel_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537:SF28">
        <property type="entry name" value="POTASSIUM VOLTAGE-GATED CHANNEL SUBFAMILY A MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11537">
        <property type="entry name" value="VOLTAGE-GATED POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02214">
        <property type="entry name" value="BTB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00520">
        <property type="entry name" value="Ion_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00169">
        <property type="entry name" value="KCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01510">
        <property type="entry name" value="KV13CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01491">
        <property type="entry name" value="KVCHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01496">
        <property type="entry name" value="SHAKERCHANEL"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="15" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0564">Palmitate</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0630">Potassium</keyword>
    <keyword evidence="7" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="289"/>
            <end position="310"/>
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    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="350"/>
            <end position="371"/>
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    </feature>
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        <location>
            <begin position="16"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
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    </evidence>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="466" mass="52812" checksum="E6687A7814761898" modified="2023-09-13" version="1">GEGAGRAGRGQDCCGERVVINISGLRFETQLKTLCQFPETLLGDPKRRMRYFDPLRNEYFFDRNRPSFDAILYYYQSGGRIRRPVNVPIDIFSEEIRFYQLGEEAMEKFREDEGFLREEERPLPRRDFQRQVWLLFEYPESSGPARGIAIVSVLVILISIVIFCLETLPEFRDEKDYPSSPFSDPFFVVETLCIIWFSFELLVRFFACPSKATFSRNIMNLIDIVAIIPYFITLGTELAERQGNGQQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGQTLKASMRELGLLIFFLFIGVILFSSAVYFAEADDPTSGFSSIPDAFWWAVVTMTTVGYGDMHPVTIGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETEGEEQAQYMHVGSCQHLSPSAEELRKARSNSTLSKSEYMVIEEGGMNHSAFPQTPFKTGNSTATCTTNNNPNSCVNIKKIFTDV</sequence>
</entry>
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            <fullName evidence="3">Coiled-coil domain containing 194</fullName>
        </submittedName>
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        <name evidence="3" type="primary">CCDC194</name>
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        <name type="scientific">Equus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
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                <person name="J."/>
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                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
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                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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            <begin position="197"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
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    <sequence length="234" mass="25097" checksum="C94FFCA46274BA92" modified="2023-09-13" version="1">MAEPGPEPGRAWRLLALCGAAVFLAAAAAGSALLAWNLAASASRGPRCPEPGANATAPPGAPVPEVEELRRRLAEAAQREEALARQLDQAEGGRRELQEALRACEGRQSLLQTQLMTLKTEMDEAKAQGTQMGVENGELTEALLRWEAAATESARQLDEAQRRARAAEAEGEACAAREAALRERVKALEAKMGPQRRVLHPRPRSGSRPRPSPRSRSRPGPSGGCRRPARRARG</sequence>
</entry>
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        <recommendedName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
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            <taxon>Equus</taxon>
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    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K4Q2"/>
    <dbReference type="Ensembl" id="ENSEAST00005053150.1">
        <property type="protein sequence ID" value="ENSEASP00005057332.1"/>
        <property type="gene ID" value="ENSEASG00005035852.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286907"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033691">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000829">
        <property type="entry name" value="Sialic acid-binding Ig-like lectin 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051036">
        <property type="entry name" value="SIGLEC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12035:SF132">
        <property type="entry name" value="MYELOID CELL SURFACE ANTIGEN CD33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12035">
        <property type="entry name" value="SIALIC ACID BINDING IMMUNOGLOBULIN-LIKE LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0430">Lectin</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Ig-like" evidence="12">
        <location>
            <begin position="9"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
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<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KA76</accession>
    <name>A0A9L0KA76_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">60 kDa heat shock protein, mitochondrial</fullName>
            <ecNumber evidence="4">5.6.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">60 kDa chaperonin</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Chaperonin 60</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Heat shock protein 60</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106824745</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homoheptamer arranged in a ring structure. The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. Interacts with 2 heptameric Hsp10 rings to form the symmetrical football complex. Interacts with HRAS. Interacts with ATAD3A. Interacts with ETFBKMT and EEF1AKMT3. Interacts with MFHAS1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the chaperonin (HSP60) family.</text>
    </comment>
    <dbReference type="EC" id="5.6.1.7" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0KA76"/>
    <dbReference type="Ensembl" id="ENSEAST00005057156.1">
        <property type="protein sequence ID" value="ENSEASP00005058175.1"/>
        <property type="gene ID" value="ENSEASG00005028912.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005727"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001819">
        <property type="term" value="P:positive regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03344">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000001">
        <property type="entry name" value="60 kDa chaperonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000011">
        <property type="entry name" value="60 kDa heat shock protein, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000026">
        <property type="entry name" value="Chaperonin 60 subunit alpha 2 chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018370">
        <property type="entry name" value="Chaperonin_Cpn60_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001844">
        <property type="entry name" value="Cpn60/GroEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02348">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000592">
        <property type="entry name" value="PRK00013.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009487">
        <property type="entry name" value="PRK12849.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009488">
        <property type="entry name" value="PRK12850.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009489">
        <property type="entry name" value="PRK12851.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45633">
        <property type="entry name" value="60 KDA HEAT SHOCK PROTEIN, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00296">
        <property type="entry name" value="CHAPERONINS_CPN60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00046475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005058175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="540" mass="58159" checksum="56D98BF590A308A1" modified="2023-09-13" version="1">MLRLPAVLRRIRPVSSALAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKEIGNIISDAMKKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSVQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEERLTLNIEDVQPHDLGKVGEVIVTKDDAMLLKGKGDKAQIEKRIQEIIEQLDVTTNGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDSIKPANEDQKIGIEIIKRTLKIPAMTIAKNAGVEGSLIVEKIMQSSSEIGYDAMLGDFVNMVEKGIIDPTKVVRTALLDAKITITISYWFQLTTYNGLLLSLSMPTDNLFCIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KAK6</accession>
    <name>A0A9L0KAK6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Shugoshin 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Shugoshin-like 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">SGO1</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytoskeleton</location>
            <location evidence="4">Spindle pole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus speckle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the shugoshin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KAK6"/>
    <dbReference type="Ensembl" id="ENSEAST00005056854.1">
        <property type="protein sequence ID" value="ENSEASP00005058305.1"/>
        <property type="gene ID" value="ENSEASG00005005948.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154107"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
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        <property type="term" value="C:nuclear speck"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:spindle pole"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:cell division"/>
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        <property type="term" value="P:meiotic chromosome segregation"/>
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    <dbReference type="FunFam" id="1.20.5.730:FF:000004">
        <property type="entry name" value="SGO1 isoform 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.20.5.730">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Shugoshin1/2"/>
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    <dbReference type="InterPro" id="IPR011516">
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    <dbReference type="PANTHER" id="PTHR21577">
        <property type="entry name" value="SHUGOSHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21577:SF3">
        <property type="entry name" value="SHUGOSHIN 1-RELATED"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="18" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="14 21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Shugoshin N-terminal coiled-coil" evidence="24">
        <location>
            <begin position="22"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="Shugoshin C-terminal" evidence="23">
        <location>
            <begin position="475"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="165"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="265"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="347"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="21">
        <location>
            <begin position="54"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="167"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="22">
        <location>
            <begin position="265"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="386"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="423"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010845"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022829"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023212"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023328"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00074224"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00083289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF07557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF07558"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005058305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="525" mass="59973" checksum="9C35AA90F38F30EB" modified="2023-09-13" version="1">MAKERCLKKSFQDSLEDIKKRMKEKRNKNLAEIGKRKSFIAAPCQIITNTSTLLKNYQDNNRMLVLALENEKSKVREAEDIILQLRKECYYLTCQLYTLKEKLTSQEAEGTAQNQEVCLSEMDSNGDNSRDLFVTDLPQVPLQEAHLPGPGESFQIEEQIPTIPNQDRQRFDLDSGEDKSTDNVLPRTVSLRRSLKKRFNNLCQFDTLDDFEISHLSGQSFELERIRFVDPLISVHIPENVEENVCQWNKDQINLSPKLIHPEKSTKTNEDILEYKSEPTKRKHRGAHGRKEKRKANRRRKSKCISKYKWSKSVNKKTVSKSKLDGSITSNDAYNFNLEEGVHLTPFRQKMSNDSTREENSYESEVSICESGGSGDDSDDLYLPTCKSSQDLTSESNRGPVTRPQSKRVLKYSAGKETEGSKPTETPTSALPETHQSSHRSLKDITNVPMDPVVKIRKLSLSPKKNKESPAASLPKRRCTASVNYKEPTLASKLRRGDPFTDLCFLNSPIFKQRKDSRCSSKKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KBS3</accession>
    <name>A0A9L0KBS3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL52</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L52, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MRPL52</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL52 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KBS3"/>
    <dbReference type="Ensembl" id="ENSEAST00005060472.1">
        <property type="protein sequence ID" value="ENSEASP00005059872.1"/>
        <property type="gene ID" value="ENSEASG00005011690.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005763"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034596">
        <property type="entry name" value="Ribosomal_mL52"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34090">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L52, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34090:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18699">
        <property type="entry name" value="MRPL52"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="125"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005059872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="150" mass="16527" checksum="B024E2DA0EDF1A12" modified="2023-09-13" version="1">MAASGFPRSVARGQRGHAHFRLLGLSMAALGTLLSTGVRRLHCSPAARAGSQWRVHQGLAANPSGYGPLTELPDWSYADGRPAPPMKGQLRRQAQREKFARRVVLLSQEMDAGLQAWQLRQQEKLQEEERKQKNALKPKGAALQSPQPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KQT1</accession>
    <name>A0A8C4KQT1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Xyloside xylosyltransferase 1</fullName>
            <ecNumber evidence="19">2.4.2.62</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">UDP-xylose:alpha-xyloside alpha-1,3-xylosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">XXYLT1</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Alpha-1,3-xylosyltransferase, which elongates the O-linked xylose-glucose disaccharide attached to EGF-like repeats in the extracellular domain of target proteins by catalyzing the addition of the second xylose. Known targets include Notch proteins and coagulation factors, such as F9.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>3-O-[alpha-D-xylosyl-(1-&gt;3)-beta-D-glucosyl]-L-seryl-[EGF-like domain protein] + UDP-alpha-D-xylose = 3-O-[alpha-D-xylosyl-(1-&gt;3)-alpha-D-xylosyl-(1-&gt;3)-beta-D-glucosyl]-L-seryl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22820"/>
            <dbReference type="Rhea" id="RHEA-COMP:14611"/>
            <dbReference type="Rhea" id="RHEA-COMP:14619"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57632"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:140575"/>
            <dbReference type="ChEBI" id="CHEBI:140599"/>
            <dbReference type="EC" id="2.4.2.62"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="18">Homodimer. Dimer formation may be essential for the retention in endoplasmic reticulum.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the glycosyltransferase 8 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.62" evidence="19"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4KQT1"/>
    <dbReference type="Ensembl" id="ENSEAST00005000905.2">
        <property type="protein sequence ID" value="ENSEASP00005000776.2"/>
        <property type="gene ID" value="ENSEASG00005000687.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158154"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140560">
        <property type="term" value="F:xylosyl alpha-1,3-xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016266">
        <property type="term" value="P:protein O-linked glycosylation via N-acetyl-galactosamine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000098">
        <property type="entry name" value="xyloside xylosyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042465">
        <property type="entry name" value="XXLT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46612">
        <property type="entry name" value="XYLOSIDE XYLOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46612:SF1">
        <property type="entry name" value="XYLOSIDE XYLOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="46"/>
            <end position="77"/>
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    <evidence type="ECO:0000256" key="1">
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        </source>
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            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00062029"/>
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        <source>
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        </source>
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        </source>
    </evidence>
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        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000776.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="380" mass="42560" checksum="B87A529FA434BE23" modified="2023-09-13" version="2">MARLGAVRSHYCALLLAAALAVCAFYYLGSGRETFSSATKRLKEARAGAPAAPSPPAPDLARGSVAPAPGAKAKSLEGGGAGPVDYHLLMMFTKAEHNAALQAKARVALRSLLHLAKFEAHEVLNLHFVSEEASREVAKALLRELLPPATGFKCKVVFHDVAVLTDKLFPVVEAMQKHFSAGSGTYYSDSIFFLSVAMHQIMPKEILRIIQLDLDLKYKTNIRELFEEFDNFLPGAIIGIAHEMQPVYRHTFWQFRHENPKTRVGSPPPEGLPGFNSGVMLLNLEAMRQSPLYSRLLEPAQVQQLADKYHFRGHLGDQDFFTMIGMEHPELFHVLDCTWNRQLCTWWRDHGYTDVFEAYFRCEGHVRIYHGNCNTPIPEV</sequence>
</entry>
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            <taxon>Laurasiatheria</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
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                <person name="Hou"/>
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                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="Guo"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
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                <person name="Yue"/>
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                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            </authorList>
            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="12" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <subcellularLocation>
            <location evidence="1 7 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KUU7"/>
    <dbReference type="Ensembl" id="ENSEAST00005002114.2">
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0000978">
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071345">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032869">
        <property type="term" value="P:cellular response to insulin stimulus"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990791">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030520">
        <property type="term" value="P:estrogen receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000165">
        <property type="term" value="P:MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904178">
        <property type="term" value="P:negative regulation of adipose tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902870">
        <property type="term" value="P:negative regulation of amacrine cell differentiation"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0050885">
        <property type="term" value="P:neuromuscular process controlling balance"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045773">
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0046326">
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        <property type="project" value="Ensembl"/>
    </dbReference>
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    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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            <end position="324"/>
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<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KV39</accession>
    <name>A0A8C4KV39_EQUAS</name>
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        <recommendedName>
            <fullName evidence="26">Glutathione hydrolase 5 proenzyme</fullName>
            <ecNumber evidence="4">2.3.2.2</ecNumber>
            <ecNumber evidence="5">3.4.19.13</ecNumber>
            <ecNumber evidence="25">3.4.19.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="31">Gamma-glutamyl leukotrienase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="27">Gamma-glutamyltransferase 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="30">Gamma-glutamyltransferase-like activity 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">Gamma-glutamyltranspeptidase 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">Leukotriene-C4 hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="34" type="primary">GGT5</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="34 35" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>S-[(2E,6E,10E)-geranylgeranyl]-L-glutathione + H2O = S-[(2E,6E,10E)-geranylgeranyl]-L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:65120"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:156326"/>
            <dbReference type="ChEBI" id="CHEBI:156330"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:65121"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>an N-terminal (5-L-glutamyl)-[peptide] + an alpha-amino acid = 5-L-glutamyl amino acid + an N-terminal L-alpha-aminoacyl-[peptide]</text>
            <dbReference type="Rhea" id="RHEA:23904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9780"/>
            <dbReference type="Rhea" id="RHEA-COMP:9795"/>
            <dbReference type="ChEBI" id="CHEBI:77644"/>
            <dbReference type="ChEBI" id="CHEBI:78597"/>
            <dbReference type="ChEBI" id="CHEBI:78599"/>
            <dbReference type="ChEBI" id="CHEBI:78608"/>
            <dbReference type="EC" id="2.3.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:23905"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>an S-substituted glutathione + H2O = an S-substituted L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:59468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:90779"/>
            <dbReference type="ChEBI" id="CHEBI:143103"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:59469"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>glutathione + H2O = L-cysteinylglycine + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:28807"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:61694"/>
            <dbReference type="EC" id="3.4.19.13"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:28808"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>leukotriene C4 + H2O = leukotriene D4 + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:31563"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57973"/>
            <dbReference type="ChEBI" id="CHEBI:63166"/>
            <dbReference type="EC" id="3.4.19.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:31564"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="24">Lipid metabolism; leukotriene D4 biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Sulfur metabolism; glutathione metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Heterodimer composed of the light and heavy chains. The active site is located in the light chain.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the gamma-glutamyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.2" evidence="4"/>
    <dbReference type="EC" id="3.4.19.13" evidence="5"/>
    <dbReference type="EC" id="3.4.19.14" evidence="25"/>
    <dbReference type="RefSeq" id="XP_014721758.1">
        <property type="nucleotide sequence ID" value="XM_014866272.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KV39"/>
    <dbReference type="Ensembl" id="ENSEAST00005000991.2">
        <property type="protein sequence ID" value="ENSEASP00005000855.2"/>
        <property type="gene ID" value="ENSEASG00005000711.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106847124"/>
    <dbReference type="KEGG" id="eai:106847124"/>
    <dbReference type="CTD" id="2687"/>
    <dbReference type="GeneTree" id="ENSGT00940000155794"/>
    <dbReference type="OrthoDB" id="1081007at2759"/>
    <dbReference type="UniPathway" id="UPA00204"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036374">
        <property type="term" value="F:glutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103068">
        <property type="term" value="F:leukotriene C4 gamma-glutamyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002951">
        <property type="term" value="F:leukotriene-C(4) hydrolase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901750">
        <property type="term" value="P:leukotriene D4 biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072676">
        <property type="term" value="P:lymphocyte migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.130:FF:000001">
        <property type="entry name" value="Gamma-glutamyltransferase 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.40:FF:000005">
        <property type="entry name" value="gamma-glutamyltransferase 5 isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055262">
        <property type="entry name" value="GGT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043138">
        <property type="entry name" value="GGT_lsub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000101">
        <property type="entry name" value="GGT_peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043137">
        <property type="entry name" value="GGT_ssub_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45027:SF2">
        <property type="entry name" value="GAMMA-GLUTAMYLTRANSFERASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45027">
        <property type="entry name" value="PUTATIVE GLUTATHIONE HYDROLASE LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01019">
        <property type="entry name" value="G_glu_transpept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01210">
        <property type="entry name" value="GGTRANSPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00462">
        <property type="entry name" value="G_GLU_TRANSPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0434">Leukotriene biosynthesis</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0645">Protease</keyword>
    <keyword evidence="35" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="15" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="32">
        <location>
            <position position="388"/>
        </location>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="110"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <begin position="406"/>
            <end position="408"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
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        </ligand>
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    <feature type="binding site" evidence="33">
        <location>
            <position position="430"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <begin position="453"/>
            <end position="454"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="33">
        <location>
            <position position="476"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
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        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    <sequence length="570" mass="60757" checksum="1F81784F03D3F849" modified="2023-09-13" version="2">MAQGRRATVGMVLLGLGLALAIIVPAVVLSRHLAHCGPQAFDHAAVAADSKVCSDIGRAILQQNGSAMDATIAALVCTGVVNPQSMGLGGGVIFTIYNATTGKVEVINARETVPARHVPDLLDQCEQAQPLGTGAQWIGVPGELRGYAEAHRRHGRLAWAQLFQPTIALLRGGYRLPPVLGQFLNHSLLRPSLNASSLRQLFFNGTEPLRPQDPFPWPTLAATLETVATEGAEVFYTGRLGQTLLEDIAKEGSWLTLQDLASFRPEVVDALAVPLGDYTLYSPPPPAGGAILSFILNVLKGFNFSAESVARPEGSVNLYHHLVETLKFAGGQRWRLWDPRSHPELQNASQVLLGEALAKHVRQQIDARGDHQLSHYSLAGATGYRMGTAHVSVLAEDGSAVAATSTINTPFGAMVYSPRTGILLNNELLDLCWRRLTGSSITPRPVAGERPPSSMVPSILVNAAQGSKLVIGGAGGELIISAVVQAIVNKLWLGFDLEAAIAAPILHVDSQGQVDYEPSFSQEVRKGLQDRGQSQSRRPFFLNVVQAVSQDGACVYAASDPRKGGKASGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KVG2</accession>
    <name>A0A8C4KVG2_EQUAS</name>
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        <recommendedName>
            <fullName evidence="11">LIM/homeobox protein Lhx1</fullName>
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        <alternativeName>
            <fullName evidence="12">Homeobox protein Lim-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LHX1</name>
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    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Potential transcription factor. May play a role in early mesoderm formation and later in lateral mesoderm differentiation and neurogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with LDB1 via the tandem LIM domains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014717756.1">
        <property type="nucleotide sequence ID" value="XM_014862270.3"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A8C4KVG2"/>
    <dbReference type="Ensembl" id="ENSEAST00005003011.2">
        <property type="protein sequence ID" value="ENSEASP00005002745.1"/>
        <property type="gene ID" value="ENSEASG00005002117.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106844685"/>
    <dbReference type="KEGG" id="eai:106844685"/>
    <dbReference type="CTD" id="3975"/>
    <dbReference type="GeneTree" id="ENSGT00940000160834"/>
    <dbReference type="OMA" id="NDQQFYP"/>
    <dbReference type="OrthoDB" id="10068367at2759"/>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001658">
        <property type="term" value="P:branching involved in ureteric bud morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021702">
        <property type="term" value="P:cerebellar Purkinje cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021937">
        <property type="term" value="P:cerebellar Purkinje cell-granule cell precursor cell signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060067">
        <property type="term" value="P:cervix development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072049">
        <property type="term" value="P:comma-shaped body morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009953">
        <property type="term" value="P:dorsal/ventral pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001705">
        <property type="term" value="P:ectoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060059">
        <property type="term" value="P:embryonic retina morphogenesis in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048703">
        <property type="term" value="P:embryonic viscerocranium morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001706">
        <property type="term" value="P:endoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021871">
        <property type="term" value="P:forebrain regionalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097477">
        <property type="term" value="P:lateral motor column neuron migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048382">
        <property type="term" value="P:mesendoderm development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072283">
        <property type="term" value="P:metanephric renal vesicle morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072284">
        <property type="term" value="P:metanephric S-shaped body morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008045">
        <property type="term" value="P:motor neuron axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035849">
        <property type="term" value="P:nephric duct elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061205">
        <property type="term" value="P:paramesonephric duct development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000744">
        <property type="term" value="P:positive regulation of anterior head development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090190">
        <property type="term" value="P:positive regulation of branching involved in ureteric bud morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="P:positive regulation of gastrulation"/>
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        <property type="term" value="P:positive regulation of nephron tubule epithelial cell differentiation"/>
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    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
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    <dbReference type="GO" id="GO:0090009">
        <property type="term" value="P:primitive streak formation"/>
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    <dbReference type="GO" id="GO:0048793">
        <property type="term" value="P:pronephros development"/>
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    <dbReference type="GO" id="GO:0010842">
        <property type="term" value="P:retina layer formation"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0032525">
        <property type="term" value="P:somite rostral/caudal axis specification"/>
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    <dbReference type="GO" id="GO:0021527">
        <property type="term" value="P:spinal cord association neuron differentiation"/>
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        <property type="term" value="P:telencephalon development"/>
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        <property type="term" value="P:transcription by RNA polymerase II"/>
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        <property type="term" value="P:ureter morphogenesis"/>
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        <property type="term" value="P:urogenital system development"/>
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        <property type="term" value="P:uterine epithelium development"/>
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        <property type="term" value="P:vagina development"/>
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        <property type="term" value="P:ventral spinal cord development"/>
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        <property type="entry name" value="homeodomain"/>
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        <property type="entry name" value="LIM1_Lhx1_Lhx5"/>
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        <property type="entry name" value="Insulin gene enhancer protein ISL-2"/>
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        <property type="entry name" value="LIM/homeobox protein Lhx1"/>
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        <property type="entry name" value="LIM/homeobox protein Lhx1"/>
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        <property type="entry name" value="Cysteine Rich Protein"/>
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        <property type="entry name" value="Homeodomain-like"/>
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        <property type="entry name" value="HD"/>
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        <property type="entry name" value="Homeobox_CS"/>
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        <property type="entry name" value="Homeodomain-like_sf"/>
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        <property type="entry name" value="LIM_Homeobox_TF"/>
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        <property type="entry name" value="Znf_LIM"/>
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        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
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        <property type="entry name" value="Homeodomain-like"/>
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    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
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    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="6 13 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 13 15" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5 14" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 13 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="17">
        <location>
            <begin position="2"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="17">
        <location>
            <begin position="62"/>
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            <begin position="178"/>
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            <begin position="180"/>
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    <sequence length="406" mass="44794" checksum="A7F7ED333998ACEB" modified="2022-01-19" version="1">MVHCAGCKRPILDRFLLNVLDRAWHVKCVQCCECKCNLTEKCFSREGKLYCKNDFFRCFGTKCAGCAQGISPSDLVRRARSKVFHLNCFTCMMCNKQLSTGEELYIIDENKFVCKEDYLSNSSVAKENSLHSATTGSDPSLSPDSQDPSQDDAKDSESANVSDKEGGSNENDDQNLGAKRRGPRTTIKAKQLETLKAAFAATPKPTRHIREQLAQETGLNMRVIQVWFQNRRSKERRMKQLSALGARRHAFFRSPRRMRPLVDRLEPGELIPNGPFSFYGDYQSEYYGPGGNYDFFPQGPPSSQAQTPVDLPFVPSSGPSGTPLGGLEHPLPGHHPSSEAQRFTDILAHPPGDSPSPEPSLPGPLHSMSAEVFGPSPPFSSLSVNGGASYGNHLSHPPEMNEAAVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KXM9</accession>
    <name>A0A8C4KXM9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PHD finger protein 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">BRG1-associated factor 45a</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">PHF10</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Component of neural progenitors-specific chromatin remodeling complex (npBAF complex) composed of at least, ARID1A/BAF250A or ARID1B/BAF250B, SMARCD1/BAF60A, SMARCD3/BAF60C, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47, SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, PHF10/BAF45A, ACTL6A/BAF53A and actin. Interacts with ACTL6A/BAF53A, SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A and PBRM1/BAF180.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAYP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KXM9"/>
    <dbReference type="Ensembl" id="ENSEAST00005000749.2">
        <property type="protein sequence ID" value="ENSEASP00005000633.2"/>
        <property type="gene ID" value="ENSEASG00005000584.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155172"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
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    <dbReference type="GO" id="GO:0071564">
        <property type="term" value="C:npBAF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15528">
        <property type="entry name" value="PHD1_PHF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15529">
        <property type="entry name" value="PHD2_PHF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21085">
        <property type="entry name" value="WH_NTD_PHF10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000202">
        <property type="entry name" value="PHD finger protein 10 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038045">
        <property type="entry name" value="PHF10_PHD_finger_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45888">
        <property type="entry name" value="HL01030P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45888:SF4">
        <property type="entry name" value="PHD FINGER PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0524">Neurogenesis</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="16">
        <location>
            <begin position="330"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="16">
        <location>
            <begin position="386"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="240"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="240"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="298"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00076725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000633.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="451" mass="51177" checksum="EEDE9963BC7E0350" modified="2023-09-13" version="2">MGSGDSSRSCETSSQDLGFSYYPAENLIEYKWPPDETGEYYMLQEQVSEYLGVTSFKRKYPDLERRDLSHKEKLYLRELNVITETQCTLGLTALRSDEVIDLMIKEYPAKHAEYSVILQEKERQRITDHYKEYSQMQQQNTQKVEASKVPEYIKKAAKKAAEFNSNLNRERMEERRAYFDLQTHVIQVPQGKYKVLPTERTKVSSYPVALIPGQFQEYYKRYSPDELRYLPLNTALYEPPLDPELPALDSDGDSDDAEDGRGDEKRKNKGTSDSSSGNVSEGEGLPDSQEEPLQGKPRSKDKGAAPRKDASKRSVLSKSVPGYKPKVIPNALCGICLKGKETNKKGKAESLIHCSQCDNSGHPSCLDMTMELVSMIKTYPWQCMECKTCIICGQPHHEEEMMFCDVCDRGYHTFCVGLGAIPSGRWICDCCQRAPPTPRKVGRRGKNSKEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KYE4</accession>
    <name>A0A8C4KYE4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Polycomb group RING finger protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">DNA-binding protein Mel-18</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">RING finger protein 110</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Zinc finger protein 144</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">PCGF2</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Within the PRC1-like complex, regulates RNF2 ubiquitin ligase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Exists as both a monomer and homodimer. Component of a PRC1-like complex. Interacts with CBX8, RING1 and RNF2. Interacts with CBX7. Interacts with PHC2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KYE4"/>
    <dbReference type="Ensembl" id="ENSEAST00005002626.2">
        <property type="protein sequence ID" value="ENSEASP00005002383.2"/>
        <property type="gene ID" value="ENSEASG00005001858.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159730"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035102">
        <property type="term" value="C:PRC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17164">
        <property type="entry name" value="RAWUL_PCGF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16736">
        <property type="entry name" value="RING-HC_PCGF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000082">
        <property type="entry name" value="Polycomb group ring finger 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000170">
        <property type="entry name" value="Polycomb group RING finger protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032443">
        <property type="entry name" value="RAWUL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10825:SF31">
        <property type="entry name" value="POLYCOMB GROUP RING FINGER PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10825">
        <property type="entry name" value="RING FINGER DOMAIN-CONTAINING, POLYCOMB GROUP COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16207">
        <property type="entry name" value="RAWUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13923">
        <property type="entry name" value="zf-C3HC4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="21">
        <location>
            <begin position="18"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="239"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00057474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00062734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00080432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00081756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005002383.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="328" mass="36493" checksum="1F7EC11BA51160B0" modified="2023-09-13" version="2">MHRTTRIKITELNPHLMCALCGGYFIDATTIVECLHSFCKTCIVRYLETNKYCPMCDVQVHKTRPLLSIRSDKTLQDIVYKLVPGLFKDEMKRRRDFYAAYPLTEVPNGSNEDRGEVLEQEKGALSDDEIVSLSIEFYEGVRDREEEKKGPLENGDGDKEKTGVRFLRCPAAMTVMHLAKFLRNKMDVPSKYKVEVLYEDEPLKEYYTLMDIAYIYPWRRNGPLPLKYRVQPACKRLTLPTAPTPSEGTNTSGASECESVSDKAPSPATLPATSSSLPSPATPSHGSPQLHGPPATTLHLPTPPSTASGTTSRGRKMTVNGAPVPPLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L1T0</accession>
    <name>A0A8C4L1T0_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Vacuole membrane protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">VMP1</name>
    </gene>
    <organism evidence="2 3">
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="309" mass="34522" checksum="B45464D1AD79AA60" modified="2022-01-19" version="1">MAKNNLTTVTEFILMGFTDYPKLEIPLFVMFLSFYLVTLLGNVGMIILIQVDVQLHTPMYFFLSHLSLLDACYISVIIPQILVTLAKGKMVISYGQCAAQFFFFTICAATECFLLAVMAYDRYVAISKPLLYTVAINPRICWSLVVVAYVCGMLGAILRTTCTFTLPFCDNNQISFFFCDLPPLLKLACNDTTSTEIVIVFFGNFVILANALVILISKAIWRVKSSGGRTKTFSTCASHLTAVALFFGTLIFMYLRSGSGKSPEEDKVVSVFYTVFIPMLNPLIYSLRNKDVKVAFRKVTGRFQGSKSM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L245</accession>
    <name>A0A8C4L245_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin-specific chaperone E</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tubulin-folding cofactor E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TBCE</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TBCE family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L245"/>
    <dbReference type="Ensembl" id="ENSEAST00005001734.2">
        <property type="protein sequence ID" value="ENSEASP00005001545.2"/>
        <property type="gene ID" value="ENSEASG00005001261.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063405"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008344">
        <property type="term" value="P:adult locomotory behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048589">
        <property type="term" value="P:developmental growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014889">
        <property type="term" value="P:muscle atrophy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048936">
        <property type="term" value="P:peripheral nervous system neuron axonogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007023">
        <property type="term" value="P:post-chaperonin tubulin folding pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009791">
        <property type="term" value="P:post-embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd17044">
        <property type="entry name" value="Ubl_TBCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.190:FF:000008">
        <property type="entry name" value="Tubulin-specific chaperone E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000268">
        <property type="entry name" value="Tubulin-specific chaperone E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000593">
        <property type="entry name" value="Tubulin-specific chaperone E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.190">
        <property type="entry name" value="CAP Gly-rich-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036859">
        <property type="entry name" value="CAP-Gly_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000938">
        <property type="entry name" value="CAP-Gly_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044079">
        <property type="entry name" value="Ubl_TBCE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849:SF0">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 410-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18849">
        <property type="entry name" value="LEUCINE RICH REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01302">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14580">
        <property type="entry name" value="LRR_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01052">
        <property type="entry name" value="CAP_GLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74924">
        <property type="entry name" value="Cap-Gly domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00845">
        <property type="entry name" value="CAP_GLY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50245">
        <property type="entry name" value="CAP_GLY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CAP-Gly" evidence="10">
        <location>
            <begin position="111"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="56"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001545.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L2K3</accession>
    <name>A0A8C4L2K3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ubiquilin-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">UBQLN3</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
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        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Mammalia</taxon>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L2K3"/>
    <dbReference type="Ensembl" id="ENSEAST00005001921.2">
        <property type="protein sequence ID" value="ENSEASP00005001725.2"/>
        <property type="gene ID" value="ENSEASG00005001387.2"/>
    </dbReference>
    <dbReference type="KEGG" id="eai:106830213"/>
    <dbReference type="GeneTree" id="ENSGT00940000162912"/>
    <dbReference type="OrthoDB" id="267397at2759"/>
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        <property type="component" value="Chromosome 20"/>
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    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14399">
        <property type="entry name" value="UBA_PLICs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01808">
        <property type="entry name" value="Ubl_PLICs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.100:FF:000001">
        <property type="entry name" value="Ubiquilin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.10:FF:000082">
        <property type="entry name" value="Ubiquilin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000198">
        <property type="entry name" value="Ubiquilin 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="A."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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            <begin position="562"/>
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        <location>
            <begin position="591"/>
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        <location>
            <begin position="603"/>
            <end position="620"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L583</accession>
    <name>A0A8C4L583_EQUAS</name>
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        <recommendedName>
            <fullName evidence="14">Cullin-4B</fullName>
        </recommendedName>
    </protein>
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        <name evidence="19" type="primary">CUL4B</name>
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    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15 16">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044619221.1">
        <property type="nucleotide sequence ID" value="XM_044763286.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L583"/>
    <dbReference type="Ensembl" id="ENSEAST00005004099.2">
        <property type="protein sequence ID" value="ENSEASP00005003736.2"/>
        <property type="gene ID" value="ENSEASG00005000286.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106824850"/>
    <dbReference type="CTD" id="8450"/>
    <dbReference type="GeneTree" id="ENSGT00940000155339"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031464">
        <property type="term" value="C:Cul4A-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031465">
        <property type="term" value="C:Cul4B-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000003">
        <property type="entry name" value="Cullin 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.130:FF:000001">
        <property type="entry name" value="Cullin 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000050">
        <property type="entry name" value="Cullin 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000004">
        <property type="entry name" value="Cullin 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000008">
        <property type="entry name" value="Cullin 4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000059">
        <property type="entry name" value="Cullin-4B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016157">
        <property type="entry name" value="Cullin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01256">
        <property type="entry name" value="CULLIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="18">
        <location>
            <begin position="539"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00068305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003736.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="894" mass="102088" checksum="471DB2FEDFD2529E" modified="2023-09-13" version="2">MKSVCPVTSGFSSPNPSAAAAAQEVRSATDGNTSTTPPTSAKKRKLNSSSSSNSSNEREDFDSASSSPPLQPRDSASPSTSSFCLGVSVAASSHVPVQKKLRFDDGVDFVGFDAKMAEESSSSSSSSSPTAATSQQQQLKNKSILISSVASVHHANGLAKSTTASSFANSKPGSAKKLVIKNFKDKPKLPENYTDETWQKLKEAVEAIQNSTSIKYNLEELYQAVENLCSYKISANLYKQLRQICEDHIKAQIHQFREDSLDSVLFLKKIDRCWQNHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRAHIISDQKVQNKTIDGILLLIERERNGEAIDRSLLRSLLSMLSDLQIYQDSFEQRFLEETNRLYAAEGQKLMQEREVPEYLHHVNKRLEEEADRLITYLDQTTQKSLIATVEKQLLGEHLTAILQKGLNNLLDENRIQDLSLLYQLFSRVRGGVQVLLQQWIEYIKAFGSTIVINPEKDKTMVQELLDFKDKVDHIIDICFLKNEKFINAMKEAFETFINKRPNKPAELIAKYVDSKLRAGNKEATDEELEKMLDKIMIIFRFIYGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIMIQFKQYMQNQNVPGNIELTVNILTMGYWPTYVPMEVHLPPEMVKLQEIFKTFYLGKHSGRKLQWQSTLGHCVLKAEFKEGKKELQVSLFQTLVLLMFNEGEEFSLEEIKQATGIEDGELRRTLQSLACGKARVLAKNPKGKDIEDGDKFICNDDFKHKLFRIKINQIQMKETVEEQASTTERVFQDRQYQIDAAIVRIMKMRKTLSHNLLVSEVYNQLKFPVKPADLKKRIESLIDRDYMERDKENPNQYNYIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L879</accession>
    <name>A0A8C4L879_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Collagen alpha-4(VI) chain-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106842755</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Collagen VI acts as a cell-binding protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the type VI collagen family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005008540.2">
        <property type="protein sequence ID" value="ENSEASP00005007827.2"/>
        <property type="gene ID" value="ENSEASG00005005705.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163168"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005589">
        <property type="term" value="C:collagen type VI trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22630">
        <property type="entry name" value="Kunitz_collagen_alpha6_VI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01472">
        <property type="entry name" value="vWA_collagen"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd01450">
        <property type="entry name" value="vWFA_subfamily_ECM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000004">
        <property type="entry name" value="collagen alpha-6(VI) chain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000003">
        <property type="entry name" value="Collagen type VI alpha 3 chain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000016">
        <property type="entry name" value="Collagen type VI alpha 3 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000021">
        <property type="entry name" value="Collagen, type VI, alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.10:FF:000020">
        <property type="entry name" value="Collagen, type VI, alpha 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.10">
        <property type="entry name" value="Pancreatic trypsin inhibitor Kunitz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050525">
        <property type="entry name" value="ECM_Assembly_Org"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002223">
        <property type="entry name" value="Kunitz_BPTI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036880">
        <property type="entry name" value="Kunitz_BPTI_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020">
        <property type="entry name" value="COLLAGEN ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24020:SF86">
        <property type="entry name" value="COLLAGEN, TYPE VI, ALPHA 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00014">
        <property type="entry name" value="Kunitz_BPTI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00453">
        <property type="entry name" value="VWFADOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00131">
        <property type="entry name" value="KU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57362">
        <property type="entry name" value="BPTI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50279">
        <property type="entry name" value="BPTI_KUNITZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="7" id="KW-0176">Collagen</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040161284" description="Collagen alpha-4(VI) chain-like" evidence="14">
        <location>
            <begin position="23"/>
            <end position="2349"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="34"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="235"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="430"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="633"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="843"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="1024"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="1770"/>
            <end position="1923"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="15">
        <location>
            <begin position="1976"/>
            <end position="2181"/>
        </location>
    </feature>
    <feature type="domain" description="BPTI/Kunitz inhibitor" evidence="16">
        <location>
            <begin position="2294"/>
            <end position="2344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1408"/>
            <end position="1713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2189"/>
            <end position="2208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="2259"/>
            <end position="2285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1408"/>
            <end position="1424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1638"/>
            <end position="1650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="1651"/>
            <end position="1660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="1694"/>
            <end position="1703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="2198"/>
            <end position="2207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
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            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
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            <dbReference type="ARBA" id="ARBA00022889"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
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    </evidence>
    <evidence type="ECO:0000256" key="8">
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            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="ARBA" id="ARBA00023278"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
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            <dbReference type="ARBA" id="ARBA00043858"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
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    <evidence type="ECO:0000313" key="17">
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    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LA99</accession>
    <name>A0A8C4LA99_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Elongation factor for RNA polymerase II 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ELL3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the ELL/occludin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LA99"/>
    <dbReference type="Ensembl" id="ENSEAST00005007233.2">
        <property type="protein sequence ID" value="ENSEASP00005006614.2"/>
        <property type="gene ID" value="ENSEASG00005004866.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161615"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008023">
        <property type="term" value="C:transcription elongation factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042771">
        <property type="term" value="P:intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="325" mass="34978" checksum="FAEE1B16B1997CB0" modified="2022-01-19" version="1">MPRPGRNTYSDQKPPYSYISLTAMAIQSSPEKMLPLSEIYKFIMDRFPYYRENTQRWQNSLRHNLSFNDCFIKIPRRPDQPGKGSFWALHPSCGDMFENGSFLRRRKRFKVLKSDHLAPSKPADAAQYLQQQAKLRLSALAASGTHLPQMPAAAYNLGGVAQPSGFKHPFAIENIIAREYKMPGGLAFSAMQPVPAAYPLPNQLTTMGSSLGTGWPHVYGSAGMIDSATPISMASGDYSAYGVPLKPLCHAAGQTLPAIPVPIKPTPAAVPALPALPAPIPTLLSNSPPSLSPTSSQTATSQSSPATPSETLTSPASALHSVAVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LCI7</accession>
    <name>A0A8C4LCI7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calmodulin-like protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CALML4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">As part of the intermicrovillar adhesion complex/IMAC plays a role in epithelial brush border differentiation, controlling microvilli organization and length. Acts as a light chain for MYO7B and is required for efficient targeting of the IMAC to the tips of border brush microvilli.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with MYO7B; the interaction mediates the association of CALML4 with the IMAC/intermicrovillar adhesion complex. Interacts with MYO7A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Microvillus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calmodulin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070355364.1">
        <property type="nucleotide sequence ID" value="XM_070499263.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070355375.1">
        <property type="nucleotide sequence ID" value="XM_070499274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LCI7"/>
    <dbReference type="Ensembl" id="ENSEAST00005009508.2">
        <property type="protein sequence ID" value="ENSEASP00005008728.1"/>
        <property type="gene ID" value="ENSEASG00005033729.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106847177"/>
    <dbReference type="CTD" id="91860"/>
    <dbReference type="GeneTree" id="ENSGT00940000157859"/>
    <dbReference type="OMA" id="NYEAFTQ"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005902">
        <property type="term" value="C:microvillus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000191">
        <property type="entry name" value="Calmodulin like 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050230">
        <property type="entry name" value="CALM/Myosin/TropC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048:SF45">
        <property type="entry name" value="CALMODULIN LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23048">
        <property type="entry name" value="MYOSIN LIGHT CHAIN 1, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="52"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="9">
        <location>
            <begin position="125"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00064623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="197" mass="22230" checksum="A9518F00C90D0133" modified="2022-01-19" version="1">MAAADLLLGPPPSLADFRLEAGRKGNREGASGSSEPRVAARGSTMAKFLSQDQINDYKECFSLYDKQQRGKIKATDLLLVMRCLGASPTPGEVQRHLQTHGLDRNGELDFSTFLTIMHMQIKQEDPKKEILLAMLMADKEKKGYIMASELRSKLMQLGEKLTHKEVDELFREANIEPNGKVKYDEFIRKITIPVQDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LDA5</accession>
    <name>A0A8C4LDA5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">small monomeric GTPase</fullName>
            <ecNumber evidence="4">3.6.5.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">RAB5A</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Early endosome membrane</location>
            <topology evidence="13">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Melanosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_014697636.1">
        <property type="nucleotide sequence ID" value="XM_014842150.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044609927.1">
        <property type="nucleotide sequence ID" value="XM_044753992.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LDA5"/>
    <dbReference type="SMR" id="A0A8C4LDA5"/>
    <dbReference type="Ensembl" id="ENSEAST00005008737.2">
        <property type="protein sequence ID" value="ENSEASP00005008007.2"/>
        <property type="gene ID" value="ENSEASG00005005818.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831752"/>
    <dbReference type="KEGG" id="eai:106831752"/>
    <dbReference type="CTD" id="5868"/>
    <dbReference type="GeneTree" id="ENSGT00940000154337"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098559">
        <property type="term" value="C:cytoplasmic side of early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032009">
        <property type="term" value="C:early phagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001726">
        <property type="term" value="C:ruffle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036477">
        <property type="term" value="C:somatodendritic compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043195">
        <property type="term" value="C:terminal bouton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003925">
        <property type="term" value="F:G protein activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019003">
        <property type="term" value="F:GDP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    <sequence length="215" mass="23659" checksum="DDDE3FD8B37C2606" modified="2023-09-13" version="2">MANRGATRPNGPNTGNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASPNIVIALSGNKADLANKRAVDFQEAQSYADDNSLLFMETSAKTSMNVNEIFMAIAKKLPKNEPQNPGANSARGRGVDLTEPTQPTRSQCCSN</sequence>
</entry>
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    <name>A0A8C4LEZ8_EQUAS</name>
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        <recommendedName>
            <fullName evidence="6">Immunoglobulin V-set domain-containing protein</fullName>
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        <name type="scientific">Equus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Yang"/>
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                <person name="Li"/>
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                <person name="Liu"/>
                <person name="W."/>
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                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="similarity">
        <text evidence="4">Belongs to the immunoglobulin superfamily. CEA family.</text>
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        <property type="entry name" value="CEA_cell_adhesion"/>
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        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="PANTHER" id="PTHR44427:SF1">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44427">
        <property type="entry name" value="CARCINOEMBRYONIC ANTIGEN-RELATED CELL ADHESION MOLECULE 19"/>
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        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040320637" description="Immunoglobulin V-set domain-containing protein" evidence="5">
        <location>
            <begin position="36"/>
            <end position="144"/>
        </location>
    </feature>
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        <location>
            <begin position="42"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00038222"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000259" key="6">
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</entry>
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    <accession>A0A8C4LG15</accession>
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        <recommendedName>
            <fullName evidence="9">Membrane-spanning 4-domains subfamily A member 4A-like</fullName>
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        <name evidence="7" type="primary">LOC106844391</name>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
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                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
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                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MS4A family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014717285.1">
        <property type="nucleotide sequence ID" value="XM_014861799.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LG15"/>
    <dbReference type="Ensembl" id="ENSEAST00005012392.2">
        <property type="protein sequence ID" value="ENSEASP00005011398.2"/>
        <property type="gene ID" value="ENSEASG00005008021.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106844391"/>
    <dbReference type="KEGG" id="eai:106844391"/>
    <dbReference type="GeneTree" id="ENSGT00940000155376"/>
    <dbReference type="OMA" id="GIKMIRS"/>
    <dbReference type="OrthoDB" id="10071849at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007237">
        <property type="entry name" value="CD20-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030417">
        <property type="entry name" value="MS4A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320:SF128">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MEMBER 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23320">
        <property type="entry name" value="MEMBRANE-SPANNING 4-DOMAINS SUBFAMILY A MS4A -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04103">
        <property type="entry name" value="CD20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="118"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="162"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011398.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="26029" checksum="9859003943E79ABA" modified="2023-09-13" version="2">MTTMQRMEQTMPGAGPNVYQLGQVADRNAYLWKGMSEKFLKGEPKVLGVVQIVIALVNLGLGIIMISVWLSFTEFYYDRPISVDSGYTIWGSVMFIISGSLSIVAGTRTTRDVVQGSLGLNITSSVLAASGIIITVISMSVFSSRYYYCHNNQKPMNCSMLVNVLMGMDGVVLILSVLEFCIAMFLSVFGCKITCCNPGRVVFPVPSNPHMAETASPVPVIGDLMPPMYQQENVPANLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LIG1</accession>
    <name>A0A8C4LIG1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Ciliary rootlet coiled-coil, rootletin family member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CROCC2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSEAST00005012620.2">
        <property type="protein sequence ID" value="ENSEASP00005011610.2"/>
        <property type="gene ID" value="ENSEASG00005008163.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162689"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055167">
        <property type="entry name" value="Rootletin-like_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159">
        <property type="entry name" value="CENTROSOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159:SF16">
        <property type="entry name" value="CILIARY ROOTLET COILED-COIL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15035">
        <property type="entry name" value="Rootletin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rootletin-like coiled-coil" evidence="4">
        <location>
            <begin position="122"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="384"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1223"/>
            <end position="1245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1311"/>
            <end position="1370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1514"/>
            <end position="1541"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="111"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="338"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="432"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="1264"/>
            <end position="1298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1341"/>
            <end position="1350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF15035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011610.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1619" mass="183192" checksum="C232DCDC6598EE08" modified="2023-09-13" version="2">MAQPRLGRGGAGLKGRTWCPHPLGVLDKLFSTFSHHCSEACQPRCLWAGVFQKLEDTILSPSASREDRALTMRGEGRQASPTPVPARIREIVASSLGEEPPQGVQEPPAPLAHVQEENELLQEELSRLEDLLAQAGAERDELASRYQAVSERLQARLQTTEARLRRSELEHSVDLEEALGRLEAAEQRSTGLSQVNALLREQLEHLKKANDTLAEELARTTGSVLHLRGELELRERTRSGEPRDFLLMWRQVTALRAHLAELRAATERGLADMRADTARTARRLHRACLNLDSHLRLSASRAADALEQRALQGTRLEQQLRDKVREMLQLQGRWDTEKVALQARLSEQMLLVEKLTEQNSKKERTISSLKMDVQKLVCGAGRQRPCAGPTEEPPTHWCPPHRGVSPPRTRSLAASDPALRAVQAAIQRWRLKEQLRLQLESSQAVAAGLREQLSESQQELRASRRLLQERAQEQAREYEDLLGKLEAQSREAQHCRATSELLGREKKALESVVEELRGKANTWDVERQRLETKNAELQRSLLLWAGQKEELVQWGQRGRRELETSQGRLEQLEEKVSRLKKELLSAREALNTAQLQRDVLESEREGLRGALARAESGSADLELLVTRLKSEGVEQRDSLAKMAALMEGLAQDKGTLNHLVLQLEQERDQLREQQKALEQERAGAQEQLAQAEQQLELVRAERRGLQQACGRLEEQLEQLEGRAAQLRRERAQLQEEVGQVTCKKQALEEQLAQSLQDQEAQMATLQRTLKEKEALSEERTQLLAQQEALERQGRLTAEEAADLRAERDSLESSLLEAQQLAMQLQSQQEQLEGEAQSARLARQALQVEMEQLKSTWEVQETKLQWDVGRLQRQVAQQERDMQLALESQALAHREDLARLQREKETLSLSLTEEKEVAARRLEQEKELLAKSAAKREVLKEEIQSLKHERDESLLQLEHEMQQALSLKEAERNLLREELSRATWELERLQQEAQSREEQAEATISATTAELKALQAQFEDAISAHQTEAAALSKSLREMAAERSNSGREAERLRAQLEEAQEGLAALRQELQGSEESREGLRREALEARQALGDETREKDVLQLSNAELRAAVRRAEQEKASFKRSKDEKEQKVLVLEEAQAAAQKQAGELRASLREAEQARGEARRELQELCRQVREPCRSPDAQRQQQSRQEALELQRQVAEGEAAQKEVLRLQRKLAEVEAGGEARERQLEERLHESRGTEQSLRAELRGVTGQLQQAGGVADSLRTRLDEACRRVHSLEQQLAHTEGARQDAESQLGRLWSTLRRGLGLGARSPSTSPERPGSPTKGSDGSEGHSGRHSASSPTRSRSPLPWPSPAPRDHGPEVDVASVRDTLRDFVQKLRDAQRERDNWRLQVASLSSRLSEAESERARAQSRMRQLQKALAEAEEGWRQAEGETSRAWAAQALQEEALQRLQTEHLASKRAASWERRRLQERLDKLHHALDESRRHSQGLAQRGRLLKEQVAGLEQRCQEAEGVQEPLQAHSEGRGREGAAGPASSSLCQEVDGALRQNLQLQLMELEQAHTLWLWEPVARHQQDLAAEAERLHGAQLQATQALESREQIHQWRVKLLEEQVGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LJ75</accession>
    <name>A0A8C4LJ75_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="22">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">USP20</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Zhuang"/>
                <person name="G."/>
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                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
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                <person name="Li"/>
                <person name="R."/>
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                <person name="M."/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="20">Interacts with VHL, leading to its ubiquitination and subsequent degradation. Interacts with CCP110. Interacts with DIO2. Interacts with HIF1A. Interacts with ADRB2. Interacts with USP18.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the peptidase C19 family. USP20/USP33 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="22"/>
    <dbReference type="RefSeq" id="XP_014718962.2">
        <property type="nucleotide sequence ID" value="XM_014863476.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070374634.1">
        <property type="nucleotide sequence ID" value="XM_070518533.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LJ75"/>
    <dbReference type="Ensembl" id="ENSEAST00005012978.2">
        <property type="protein sequence ID" value="ENSEASP00005011943.1"/>
        <property type="gene ID" value="ENSEASG00005008368.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106845401"/>
    <dbReference type="KEGG" id="eai:106845401"/>
    <dbReference type="CTD" id="10868"/>
    <dbReference type="GeneTree" id="ENSGT00940000158829"/>
    <dbReference type="OMA" id="IDQDDEC"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001664">
        <property type="term" value="F:G protein-coupled receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140374">
        <property type="term" value="P:antiviral innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007249">
        <property type="term" value="P:canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043124">
        <property type="term" value="P:negative regulation of canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010508">
        <property type="term" value="P:positive regulation of autophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071108">
        <property type="term" value="P:protein K48-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070536">
        <property type="term" value="P:protein K63-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008277">
        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2230.10:FF:000001">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2230.10:FF:000002">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000065">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000120">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2230.10">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF13">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00695">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="16" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="14 22" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 22" id="KW-0645">Protease</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="15 22" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="18" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="13 22" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="17" id="KW-0862">Zinc</keyword>
    <keyword evidence="12 21" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="25">
        <location>
            <begin position="6"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="24">
        <location>
            <begin position="145"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="DUSP" evidence="26">
        <location>
            <begin position="688"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="domain" description="DUSP" evidence="26">
        <location>
            <begin position="790"/>
            <end position="893"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="259"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="361"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="317"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="363"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00008269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00061890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="915" mass="102036" checksum="E5A7DB8DEFBB8B79" modified="2022-01-19" version="1">MGDSRDLCPHLDSIGEVTKEDLLLKSKGTCQSCGVAGPNLWACLQVACPYVGCGESFADHSTIHAQVKKHNLTVNLTTFRVWCYACEREVFLEQRLAAHPPGPSPKFSEQDAPLPSHPLKAVPIAVADEGESESEDDDLKPRGLTGMKNLGNSCYMNAALQALSNCPPLTQFFLECGGLVRTDKKPALCKSYQKLVSEVWHRKRPSYVVPTSLSHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPVVAAAAALTEARDSDSSDSDEKREGDRSPSEDEFLSCDSSSDRGEGDGQGRGGGGSQAETELLIPDEAGRAISEKERMKDRKFSWGQQRTNSEQVDEDADVDTAMTALDHQPTDAQAPSPRSASPCRTPEPDNEAHMRSASRSCSPVHHHEGHAKLASSPPRASPVRMGPSYVLKKAQVLSAGSRRRKEQRYRSVISDIFDGSILSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHSAIYQNVPAKPGACGDSYAAQGWLAFIMEYIRRFVVSCTPSWFWGPVVTLEDCLAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLRLPEILCIHLKRFRHEVMYSFKISSHVSFPLEGLDLRPFLAKECTSQITSYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNAEAYVLFYRKSSEEAVRERQQVVSLAAMREPSLLRFYVSREWLNKFNTFAEPGPITNHTFLCSHGGIPPNKYHYIDDLVVILPQNVWEHLYNRFGGGPAVNHLYVCSICQVEIEALAKRRRIEIDTFIKLNKAFQAEESPSVIYCISMQWFREWEAFVKGKDNEPPGPIDNSRIAQVKGSGHIQLKQGADYGQISEETWGYLSSLYGGGPELAIRQSVAQLQDPESLHGEQKIEAETRAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LLI7</accession>
    <name>A0A8C4LLI7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospholipase B1, membrane-associated</fullName>
            <ecNumber evidence="5">3.1.1.3</ecNumber>
            <ecNumber evidence="4">3.1.1.4</ecNumber>
            <ecNumber evidence="3">3.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Lysophospholipase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Phospholipase A2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Phospholipase B/lipase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Triacylglycerol lipase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="50" type="primary">PLB1</name>
    </gene>
    <organism evidence="50 51">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="50 51" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="50" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="50" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="24">Calcium-independent membrane-associated phospholipase that catalyzes complete diacylation of phospholipids by hydrolyzing both sn-1 and sn-2 fatty acyl chains attached to the glycerol backbone (phospholipase B activity). Has dual phospholipase and lysophospholipase activities toward diacylphospholipids. Preferentially cleaves sn-2 ester bonds over sn-1 bonds. Acts as a lipase toward glycerolipid substrates. Hydrolyzes fatty acyl chains of diacylglycerols with preference for the sn-2 position and of triacylglycerols with not positional selectivity. May also hydrolyze long chain retinyl esters such as retinyl palmitate. May contribute to digestion of dietary phospholipids, glycerolipids and retinoids, facilitating lipid absorption at the brush border.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>1,2,3-tri-(9Z-octadecenoyl)-glycerol + H2O = di-(9Z)-octadecenoylglycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38575"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:53753"/>
            <dbReference type="ChEBI" id="CHEBI:75945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="36">
            <dbReference type="Rhea" id="RHEA:38576"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40923"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28610"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:74669"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:40924"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="44">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + 2 H2O = sn-glycerol 3-phosphocholine + 2 hexadecanoate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:40975"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="44">
            <dbReference type="Rhea" id="RHEA:40976"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>1,2-dihexadecanoyl-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41223"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:72999"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:41224"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="45">
            <text>1,3-di-(9Z-octadecenoyl)-glycerol + H2O = 1-(9Z-octadecenoyl)-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:39939"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75342"/>
            <dbReference type="ChEBI" id="CHEBI:75735"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="45">
            <dbReference type="Rhea" id="RHEA:39940"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>1,3-dihexadecanoyl-2-(9Z-octadecenoyl)glycerol + H2O = 1,3-dihexadecanoylglycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40983"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75688"/>
            <dbReference type="ChEBI" id="CHEBI:77619"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="41">
            <dbReference type="Rhea" id="RHEA:40984"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>1,3-dihexadecanoyl-2-(9Z-octadecenoyl)glycerol + H2O = 1-hexadecanoyl-2-(9Z-octadecenoyl)-glycerol + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40979"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:75585"/>
            <dbReference type="ChEBI" id="CHEBI:75688"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:40980"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>1-(9Z-octadecenoyl)-glycerol + H2O = glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38487"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75342"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:38488"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="42">
            <text>1-O-hexadecyl-2-(9Z)-octadecenoyl-sn-glycero-3-phosphocholine + H2O = 1-O-hexadecyl-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40915"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:34112"/>
            <dbReference type="ChEBI" id="CHEBI:64496"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="42">
            <dbReference type="Rhea" id="RHEA:40916"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>1-hexadecanoyl-2-(9Z)-octadecenoyl-3-octadecanoyl-sn-glycerol + H2O = 1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + octadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41111"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:75466"/>
            <dbReference type="ChEBI" id="CHEBI:77623"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:41112"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="43">
            <text>1-hexadecanoyl-2-(9Z)-octadecenoyl-3-octadecanoyl-sn-glycerol + H2O = 1-hexadecanoyl-3-octadecanoyl-sn-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41103"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:77623"/>
            <dbReference type="ChEBI" id="CHEBI:77624"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="43">
            <dbReference type="Rhea" id="RHEA:41104"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>1-hexadecanoyl-2-(9Z)-octadecenoyl-3-octadecanoyl-sn-glycerol + H2O = 2-(9Z-octadecenoyl)-3-octadecanoyl-sn-glycerol + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:41107"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:75558"/>
            <dbReference type="ChEBI" id="CHEBI:77623"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:41108"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = (9Z,12Z)-octadecadienoate + 1-hexadecanoyl-sn-glycero-3-phosphocholine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40811"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73002"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:40812"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + H2O = 2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40971"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:73002"/>
            <dbReference type="ChEBI" id="CHEBI:76084"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:40972"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol) + H2O = 1-hexadecanoyl-sn-glycero-3-phospho-(1'-sn-glycerol) + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40919"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:72841"/>
            <dbReference type="ChEBI" id="CHEBI:75158"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:40920"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphocholine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38779"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
            <dbReference type="ChEBI" id="CHEBI:73001"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="40">
            <dbReference type="Rhea" id="RHEA:38780"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>1-hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine + H2O = 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40911"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:73004"/>
            <dbReference type="ChEBI" id="CHEBI:73007"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="38">
            <dbReference type="Rhea" id="RHEA:40912"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40435"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:72998"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:40436"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>1-octadecanoyl-2-(9Z,12Z)-octadecadienoyl-sn-glycerol + H2O = 1-octadecanoyl-sn-glycerol + (9Z,12Z)-octadecadienoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40927"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:75550"/>
            <dbReference type="ChEBI" id="CHEBI:77097"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:40928"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>2,3-di-(9Z)-octadecenoyl-sn-glycerol + H2O = 3-(9Z-octadecenoyl)-sn-glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:75824"/>
            <dbReference type="ChEBI" id="CHEBI:75938"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:42605"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="46">
            <text>2-(9Z-octadecenoyl)-glycerol + H2O = glycerol + (9Z)-octadecenoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38491"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:73990"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="46">
            <dbReference type="Rhea" id="RHEA:38492"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15801"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:57643"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:15802"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>a 1-O-alkyl-2-acyl-sn-glycero-3-phosphocholine + H2O = a 1-O-alkyl-sn-glycero-3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36231"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:30909"/>
            <dbReference type="ChEBI" id="CHEBI:36702"/>
            <dbReference type="EC" id="3.1.1.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:36232"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:15178"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:12045"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 'GDSL' lipolytic enzyme family. Phospholipase B1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.3" evidence="5"/>
    <dbReference type="EC" id="3.1.1.4" evidence="4"/>
    <dbReference type="EC" id="3.1.1.5" evidence="3"/>
    <dbReference type="Ensembl" id="ENSEAST00005014107.2">
        <property type="protein sequence ID" value="ENSEASP00005012978.2"/>
        <property type="gene ID" value="ENSEASG00005009061.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063883"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031526">
        <property type="term" value="C:brush border membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:phospholipase A2 activity"/>
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    <dbReference type="GO" id="GO:0050253">
        <property type="term" value="F:retinyl-palmitate esterase activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
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    <dbReference type="GO" id="GO:0006644">
        <property type="term" value="P:phospholipid metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000344">
        <property type="term" value="P:positive regulation of acrosome reaction"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01824">
        <property type="entry name" value="Phospholipase_B_like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008265">
        <property type="entry name" value="Lipase_GDSL_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035547">
        <property type="entry name" value="Phospholipase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038885">
        <property type="entry name" value="PLB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21325">
        <property type="entry name" value="PHOSPHOLIPASE B, PLB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21325:SF52">
        <property type="entry name" value="PHOSPHOLIPASE B1, MEMBRANE-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01098">
        <property type="entry name" value="LIPASE_GDSL_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 48" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="51" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 49" id="KW-0732">Signal</keyword>
    <keyword evidence="8 48" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 48" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="49">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040121713" description="Phospholipase B1, membrane-associated" evidence="49">
        <location>
            <begin position="24"/>
            <end position="1457"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="48">
        <location>
            <begin position="1417"/>
            <end position="1440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="47">
        <location>
            <begin position="708"/>
            <end position="728"/>
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    </feature>
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            <dbReference type="ARBA" id="ARBA00004247"/>
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    <evidence type="ECO:0000256" key="2">
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<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSEASP00005012304.2"/>
        <property type="gene ID" value="ENSEASG00005008582.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006933"/>
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        <property type="component" value="Chromosome 13"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:tRNA-queuosine(34) galactosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916:SF3">
        <property type="entry name" value="UDP-GLCNAC:BETAGAL BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="2">
        <location>
            <begin position="13"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="354"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005012304.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="366" mass="41585" checksum="6C6D37A5282740B2" modified="2023-09-13" version="2">MEAGGQPAAAHVSVILPVHNAEAWLDECLRSVLQQDFEGSMELSVFNDASKDKSMTIIEKWKVKLEDSGILVVIGGHDSPSPRGVGYSKNQAIAQSSGSYLCFLDSDDAMMPQRVRLQHEAAVRHPTSIIGCQVRREPPNSTERYTRWINQLASDQLLTQVFTSHGPTVVMPTWFCSRAWFSHVGPFDEGGQGVPEDLLFFYSHLRKGGGVVRVDQSLLIYRYHPKAATHSVLETTIWTHRVRFLEERALPHWAAFTIWNAGRQGRRLYRSLTAESRRKVVAFCDVDENKIKKGFYSYEDSEERPKPRVPILHFRAARPPFVICVKLAARFVVAGYTQQITDMDPRWQNSEENKPHRHPCRSLTLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LTQ0</accession>
    <name>A0A8C4LTQ0_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Olfactory receptor family 9 subfamily A member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">OR9A4</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LTQ0"/>
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        <property type="protein sequence ID" value="ENSEASP00005014141.2"/>
        <property type="gene ID" value="ENSEASG00005033752.1"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047132">
        <property type="entry name" value="Olfact_rcpt_6C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454:SF18">
        <property type="entry name" value="OLFACTORY RECEPTOR 6C76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="25"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="59"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="101"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="197"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="40"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014141.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="314" mass="35368" checksum="D4EC8BA98437F595" modified="2023-09-13" version="2">MLGNQSSATEFYLLGFPGSEELRHILFAIFFFLYSVTLMGNTVIIVIVCVDKRLKSPMYFFLGHLSALEILVTSIIVPVMLWGLLLPEMQTISLTACVTQLFLYLSVGTTEFVLLGAMAVDLYAAVCNPLRYNIIMNSRTCNFVVIVPWVFGFLFQIWPVYATFHLSFCKSNVVNSFSCDRGQLLKLSCNNTLFMEFILFLMAVFVPFGSLMPTFISYTYVISTILKIPSASGRRKAFSTCASHCTFVVIGYGSCLFLYMKPKQTQAAEYNRAVSLLLSVVTPFLNPFIFTLRNDKVIEALRDGGKRCCQLFRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LWE2</accession>
    <name>A0A8C4LWE2_EQUAS</name>
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        <recommendedName>
            <fullName evidence="14">Interleukin-6 receptor subunit beta</fullName>
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            <fullName evidence="16">Interleukin-6 signal transducer</fullName>
        </alternativeName>
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        <alternativeName>
            <fullName evidence="17">Oncostatin-M receptor subunit alpha</fullName>
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    </protein>
    <gene>
        <name evidence="21" type="primary">IL6ST</name>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type I cytokine receptor family. Type 2 subfamily.</text>
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    <dbReference type="RefSeq" id="XP_014715853.2">
        <property type="nucleotide sequence ID" value="XM_014860367.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A8C4LWE2"/>
    <dbReference type="Ensembl" id="ENSEAST00005018868.2">
        <property type="protein sequence ID" value="ENSEASP00005017368.2"/>
        <property type="gene ID" value="ENSEASG00005012022.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106843367"/>
    <dbReference type="CTD" id="3572"/>
    <dbReference type="GeneTree" id="ENSGT00940000159608"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070110">
        <property type="term" value="C:ciliary neurotrophic factor receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005896">
        <property type="term" value="C:interleukin-6 receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005900">
        <property type="term" value="C:oncostatin-M receptor complex"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005127">
        <property type="term" value="F:ciliary neurotrophic factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015026">
        <property type="term" value="F:coreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019970">
        <property type="term" value="F:interleukin-11 binding"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
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                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
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                <person name="M."/>
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                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <property type="entry name" value="ADP-RIBOSYLATION-LIKE FACTOR 6-INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20952:SF4">
        <property type="entry name" value="RETICULOPHAGY REGULATOR 2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF24456">
        <property type="entry name" value="RHD_RETREG1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="RETREG1-3/ARL6IP-like N-terminal reticulon-homology" evidence="7">
        <location>
            <begin position="61"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="252"/>
            <end position="285"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="334"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="497"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="273"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="459"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="512"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    <evidence type="ECO:0000256" key="5">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000256" key="6">
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            <dbReference type="SAM" id="Phobius"/>
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    <evidence type="ECO:0000313" key="9">
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    </evidence>
    <sequence length="544" mass="57863" checksum="A83B12CE347CBBA4" modified="2023-09-13" version="2">MASGGGGGNSGAAGGPGLGLGLGLGLSLGMSEATGEAEEEAATAEAVGRLATALWLRLRGWEAVLAAAQRLLVWEKPLHSLVTAAALNGLFWLLSSSCLRPIFLLSISLLAYFLLDLWQPRFLPDVSASTPEEPHSDSEGAGSGARPHLLSVPELCRYLAESWLTFQIHLQELLQYKRQNPAQFCARVCSACAVLAVLGHYVPGIMISYIVLLSILLWPLVVYHELIQRMYTRLEPLLMQLDYSMKAEADALHHKHDKKKRQGKNAPPGGDEPLAETESESEAELAGFSPVVDVKKTALALAITDSELSDEEASILESGGFSVSRATTPQLTDVSEDLDQQSLPSEPEEALSRELGEGEETELAPPEDLLGSPQAISRQDLDSEEEEEDVAAKETLLRLSSPLHFVNTHFNGAGSPTDGVKLSPGGPVETLSPEAAGGDLTPPPSTLSPLLRLAESDPVPSPSVLPPLPQDSPQPLPAPEEEEALTTEDFELLDQGELEQLNAELGLGPETSPEPPDAPPPPPLGPDTLSLVQSDQEAQAVAEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LZB3</accession>
    <name>A0A8C4LZB3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Integrin beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="33" type="primary">ITGB3</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="26">Cell membrane</location>
            <topology evidence="26">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Lamellipodium membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Postsynaptic cell membrane</location>
            <topology evidence="24">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 26">Belongs to the integrin beta chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044600946.1">
        <property type="nucleotide sequence ID" value="XM_044745011.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LZB3"/>
    <dbReference type="Ensembl" id="ENSEAST00005016489.2">
        <property type="protein sequence ID" value="ENSEASP00005015155.2"/>
        <property type="gene ID" value="ENSEASG00005010592.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106843793"/>
    <dbReference type="KEGG" id="eai:106843793"/>
    <dbReference type="CTD" id="3690"/>
    <dbReference type="GeneTree" id="ENSGT01150000286919"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071133">
        <property type="term" value="C:alpha9-beta1 integrin-ADAM8 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035868">
        <property type="term" value="C:alphav-beta3 integrin-HMGB1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035867">
        <property type="term" value="C:alphav-beta3 integrin-IGF-1-IGF1R complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035866">
        <property type="term" value="C:alphav-beta3 integrin-PKCalpha complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031527">
        <property type="term" value="C:filopodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070442">
        <property type="term" value="C:integrin alphaIIb-beta3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034683">
        <property type="term" value="C:integrin alphav-beta3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031258">
        <property type="term" value="C:lamellipodium membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031528">
        <property type="term" value="C:microvillus membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019960">
        <property type="term" value="F:C-X3-C chemokine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050840">
        <property type="term" value="F:extracellular matrix binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070051">
        <property type="term" value="F:fibrinogen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017134">
        <property type="term" value="F:fibroblast growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001968">
        <property type="term" value="F:fibronectin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031994">
        <property type="term" value="F:insulin-like growth factor I binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038132">
        <property type="term" value="F:neuregulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003756">
        <property type="term" value="F:protein disulfide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005080">
        <property type="term" value="F:protein kinase C binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043184">
        <property type="term" value="F:vascular endothelial growth factor receptor 2 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038027">
        <property type="term" value="P:apolipoprotein A-I-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043277">
        <property type="term" value="P:apoptotic cell clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072378">
        <property type="term" value="P:blood coagulation, fibrin clot formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033627">
        <property type="term" value="P:cell adhesion mediated by integrin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007044">
        <property type="term" value="P:cell-substrate junction assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036120">
        <property type="term" value="P:cellular response to platelet-derived growth factor stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034113">
        <property type="term" value="P:heterotypic cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048333">
        <property type="term" value="P:mesodermal cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050919">
        <property type="term" value="P:negative chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010888">
        <property type="term" value="P:negative regulation of lipid storage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032369">
        <property type="term" value="P:negative regulation of lipid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050748">
        <property type="term" value="P:negative regulation of lipoprotein metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010989">
        <property type="term" value="P:negative regulation of low-density lipoprotein particle clearance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010745">
        <property type="term" value="P:negative regulation of macrophage derived foam cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070527">
        <property type="term" value="P:platelet aggregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048008">
        <property type="term" value="P:platelet-derived growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001954">
        <property type="term" value="P:positive regulation of cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010595">
        <property type="term" value="P:positive regulation of endothelial cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001938">
        <property type="term" value="P:positive regulation of endothelial cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010763">
        <property type="term" value="P:positive regulation of fibroblast migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048146">
        <property type="term" value="P:positive regulation of fibroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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    <sequence length="784" mass="86504" checksum="15246E59BE8642B6" modified="2023-09-13" version="2">MRARLLWAAMLVLGALAGVGVGGPNICTTRGVSSCRQCLAASPMCAWCSDEALPLGSPRCNLKENLLKDNCALESIEFPVSEAIILEARPLSDKGSGDSSQITQVSPQRIALRLRPDDSKNFSVQVRQVEDYPVDIYYLMDLSYSMKDDLGSIQNLGTKLASQMRKLTSNLRIGFGAFVDKPVSPYMFISPPEALKNPCYDMKNVCLPMFGYKHVLTLTDQVTRFNEEVKKQSVSRNRDAPEGGFDAIMQATVCDKKIGWRNDASHLLVFTTDAKTHIALDGRLAGIVQPNDGQCHIGSDNHYSASTTMDYPSLGLMTEKLSQKNINLIFAVTENVVNLYQNYSELIPGTTVGVLSTDSSNVLQLIVDAYGKIRSKVELEVRDLPEELSLSFNATCLNNEVIPGLKSCVGLKIGDTVSFSIEAKVRGCPQEKEKSFTIKPVGFKDSLTVQVTFDCDCACQAWAEPYSHRCNNGNGTFECGVCRCGPGWLGSQCECSEEDYRPSQQDECSPREGQPICSQRGECLCGQCVCHTSDFGKITGKYCECDDFSCVRYKGELCSGHGQCSCGDCLCDSDWTGYYCNCTTRTDTCMSSNGLLCSGRGKCECGSCVCIQPGSYGDTCEKCPTCPDACTFKKECVECKKFERGALYEENTCTRYCRDEIESVKELMDTGKDAVNCTYKNEDDCVVRFQYYEDASGKSILYVVEEPECPKGPDILVVLLSVMGAILLIGLATLLIWKLLITIHDRKEFAKFEEERARAKWDTANNPLYKEATSTFTNITYRGT</sequence>
</entry>
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    <name>A0A8C4M1E1_EQUAS</name>
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        <submittedName>
            <fullName evidence="2">Chromosome 1 open reading frame 131</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">C1orf131</name>
        <name evidence="2" type="synonym">FSAF1</name>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="Ensembl" id="ENSEAST00005020415.2">
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        <property type="gene ID" value="ENSEASG00005012965.2"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="FSAF1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052852">
        <property type="entry name" value="SSU_Processome_Comp"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28366">
        <property type="entry name" value="CHROMOSOME 1 OPEN READING FRAME 131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28366:SF1">
        <property type="entry name" value="CHROMOSOME 1 OPEN READING FRAME 131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15375">
        <property type="entry name" value="FSAF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005018814.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="365" mass="40790" checksum="47731D8563C23DAF" modified="2023-09-13" version="2">MVLLLGAVLPRREARPRLRQWHPLRPLHRTPGAAILTRTEAARGPAARARSPSEPGSGGSTRLESRPPLGCTAAADPTMAQEQDLGSSAPACSQTLLDAVLQNLYDFGETEEEAEQKRIRKKRENKKRDEGTSVVLAAEPAPQLSSLVRGQRKSASSFFKELREELQCAPAVTSTGSPLGPEVPSASVSPPLKKNRERVQVVEFHSRNKKRKQKPDQDENRKTKTSILEKDVDIQEFNLEKARLEVHRFGITGYGKGKERVLEQERAIMLGAKPPKNSYVNYKVLQEQIKEKKAAKEEEKRRAQDTDIFKKKKRKGQEDRKSKRKKSAPSILSSGRTGQIGKFKNGTLILSRVDIKKINSSRVPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M2S6</accession>
    <name>A0A8C4M2S6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Mannose-binding protein C</fullName>
        </recommendedName>
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    <gene>
        <name evidence="20" type="primary">MBL2</name>
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    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
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    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Tian"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4M2S6"/>
    <dbReference type="Ensembl" id="ENSEAST00005020640.2">
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        <property type="gene ID" value="ENSEASG00005013094.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154368"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005581">
        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005537">
        <property type="term" value="F:D-mannose binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038187">
        <property type="term" value="F:pattern recognition receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001905">
        <property type="term" value="P:activation of membrane attack complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140374">
        <property type="term" value="P:antiviral innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002752">
        <property type="term" value="P:cell surface pattern recognition receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006958">
        <property type="term" value="P:complement activation, classical pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001867">
        <property type="term" value="P:complement activation, lectin pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050830">
        <property type="term" value="P:defense response to Gram-positive bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002233">
        <property type="term" value="P:leukocyte chemotaxis involved in immune response"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903028">
        <property type="term" value="P:positive regulation of opsonization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
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        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
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        <property type="entry name" value="Ca-dependent_lectin"/>
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    </dbReference>
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        <property type="entry name" value="CTDL_fold"/>
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    <dbReference type="PANTHER" id="PTHR24024:SF34">
        <property type="entry name" value="MANNOSE-BINDING PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024">
        <property type="entry name" value="PULMONARY SURFACTANT-ASSOCIATED PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
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</entry>
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                <person name="Guo"/>
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</entry>
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    <accession>A0A8C4MC36</accession>
    <name>A0A8C4MC36_EQUAS</name>
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            <fullName evidence="11">Ubiquitin carboxyl-terminal hydrolase</fullName>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="11"/>
    <dbReference type="RefSeq" id="XP_044619335.1">
        <property type="nucleotide sequence ID" value="XM_044763400.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MC36"/>
    <dbReference type="Ensembl" id="ENSEAST00005022640.2">
        <property type="protein sequence ID" value="ENSEASP00005020857.2"/>
        <property type="gene ID" value="ENSEASG00005014306.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106847991"/>
    <dbReference type="KEGG" id="eai:106847991"/>
    <dbReference type="CTD" id="8237"/>
    <dbReference type="GeneTree" id="ENSGT00940000160485"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02674">
        <property type="entry name" value="Peptidase_C19R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000013">
        <property type="entry name" value="ubiquitin carboxyl-terminal hydrolase 15 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2230.10">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028135">
        <property type="entry name" value="Ub_USP-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029346">
        <property type="entry name" value="USP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF29">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14836">
        <property type="entry name" value="Ubiquitin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14533">
        <property type="entry name" value="USP7_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00695">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6 11" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DUSP" evidence="14">
        <location>
            <begin position="25"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="13">
        <location>
            <begin position="256"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="228"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="592"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="887"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="596"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="889"/>
            <end position="909"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020857.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="915" mass="104000" checksum="FE2213C959AFE8DA" modified="2023-09-13" version="2">MAAVAADPAAVDAAEDQEPQRKAVPGLESQRRQIKSSESGRERPLRAGESWFLVEQHWYQQWELYVQGGDQDSSTFPGCINNAKLFEDQVNWRLKKGLVEGEDYVLLPEAAWHYLVSWYGLEHGQPPIERKVVELLSIQEVEVYPVELLLVQHSDMDTPCTAQFSHTDSVDLVLRTAREQFLVSPQEETRLWVKNAEGSFERLCNTRVTVLDAALKTGQLVVMETRNKDGTWPSAQPHTTKSASEDENFQGQPGICGLTNLGNTCFMNSALQCLSNVPQLTEYFLKNRYLEELNFCNPLGMKGEIAEAYADLVKQAWSGHHRSIVPHVFKTKVGHFASQFLGYQQHDSQELLSFLLDGLHEDLNRVKKKEYVELCDAAGRPDQEVAQEAWQNHKRRNDSVIVDTFHGLFKSTLVCPDCGNVSVTFDPFCYLSVPLPVSHKRVMEVFFVSMDPRRKPEQHRLVVPKKGKISDLCVALAKHTGVSPERMMVADVFSHRFYKIYQLEESLSSILDRDDIFIYEVSGRSAIGENSREDVVLPIYLRERTPARDYNNSYYGLMLFGHPLLVSVPRDRLSWDALYHILLYRLSRYVTRPSSDDEDDGDEKDLEDKDNIPKPGHVAGGSSQDPGPEQAGPSSGVAGGSRAPVDNSPGPSHWPQRARRKHLFTLQTVNSNGTSDRSTFNEDTHGVSFSSQPYIAIDWEPEMKKRYYDEVEAEGYVKHDCVGYVLKKAPVRLQECIELFTTVETLEKENPWYCPSCKQHQLATKKLDLWMLPETLIIHLKRFSYTKFSREKLDTLVEFPIRDLDFSEFVIKPQNESAPELYKYDLIAVSNHYGGLRDGHYTTFACNKDSGQWHYFDDSSVSPVTENQIESKAAYVLFYQRQDVARRLQPQPASSDPPASLGSSSPPNSEFMDVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MCG3</accession>
    <name>A0A8C4MCG3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ameloblastin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">AMBN</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the mineralization and structural organization of enamel.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ameloblastin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MCG3"/>
    <dbReference type="Ensembl" id="ENSEAST00005026668.2">
        <property type="protein sequence ID" value="ENSEASP00005024589.2"/>
        <property type="gene ID" value="ENSEASG00005016708.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018227"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030345">
        <property type="term" value="F:structural constituent of tooth enamel"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031214">
        <property type="term" value="P:biomineral tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042475">
        <property type="term" value="P:odontogenesis of dentin-containing tooth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007798">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Liu"/>
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                <person name="Wu"/>
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                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MK54"/>
    <dbReference type="Ensembl" id="ENSEAST00005030398.2">
        <property type="protein sequence ID" value="ENSEASP00005027987.2"/>
        <property type="gene ID" value="ENSEASG00005019030.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162923"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05716">
        <property type="entry name" value="IgV_pIgR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000370">
        <property type="entry name" value="CMRF35-like molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF89">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <feature type="domain" description="Ig-like" evidence="6">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005027987.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="188" mass="21007" checksum="7EC91C1665C20EBF" modified="2023-09-13" version="2">MGTTGGSLSVQCHYEEMYKGYNKYWCRGQYDTACNIIVETKGEEREERNGRVSIRDCADKLTFTVTMENLNADDAGTYWCRIQTVWILDAWSYDPSVQVKVFVFPASSTTTGRTTRPAAPATLPVVNTRQNVSTGEVLTHSSGTDTLRVLAQSCRLQILKPQPHLPGMMRRSRAGNSHMVLPAWVLSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MKB1</accession>
    <name>A0A8C4MKB1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Neuroligin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">NLGN2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the type-B carboxylesterase/lipase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MKB1"/>
    <dbReference type="Ensembl" id="ENSEAST00005030474.2">
        <property type="protein sequence ID" value="ENSEASP00005028059.2"/>
        <property type="gene ID" value="ENSEASG00005019070.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160598"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098691">
        <property type="term" value="C:dopaminergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098982">
        <property type="term" value="C:GABA-ergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098690">
        <property type="term" value="C:glycinergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099634">
        <property type="term" value="C:postsynaptic specialization membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042043">
        <property type="term" value="F:neurexin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097116">
        <property type="term" value="P:gephyrin clustering involved in postsynaptic density assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904862">
        <property type="term" value="P:inhibitory synapse assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035641">
        <property type="term" value="P:locomotory exploration behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050885">
        <property type="term" value="P:neuromuscular process controlling balance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072578">
        <property type="term" value="P:neurotransmitter-gated ion channel clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000463">
        <property type="term" value="P:positive regulation of excitatory postsynaptic potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097151">
        <property type="term" value="P:positive regulation of inhibitory postsynaptic potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032024">
        <property type="term" value="P:positive regulation of insulin secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902474">
        <property type="term" value="P:positive regulation of protein localization to synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032230">
        <property type="term" value="P:positive regulation of synaptic transmission, GABAergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051968">
        <property type="term" value="P:positive regulation of synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000809">
        <property type="term" value="P:positive regulation of synaptic vesicle clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097119">
        <property type="term" value="P:postsynaptic density protein 95 clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097104">
        <property type="term" value="P:postsynaptic membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097105">
        <property type="term" value="P:presynaptic membrane assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034394">
        <property type="term" value="P:protein localization to cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002087">
        <property type="term" value="P:regulation of respiratory gaseous exchange by nervous system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019233">
        <property type="term" value="P:sensory perception of pain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051932">
        <property type="term" value="P:synaptic transmission, GABAergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002018">
        <property type="entry name" value="CarbesteraseB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019819">
        <property type="entry name" value="Carboxylesterase_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051093">
        <property type="entry name" value="Neuroligin/BSAL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000460">
        <property type="entry name" value="Nlgn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43903">
        <property type="entry name" value="NEUROLIGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00135">
        <property type="entry name" value="COesterase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01090">
        <property type="entry name" value="NEUROLIGIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00941">
        <property type="entry name" value="CARBOXYLESTERASE_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="640"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="13">
        <location>
            <begin position="42"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="Carboxylesterase type B" evidence="13">
        <location>
            <begin position="310"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="587"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="674"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="752"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="594"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="680"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="759"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="786"/>
            <end position="799"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005028059.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="799" mass="86791" checksum="B7EB20861CD93944" modified="2023-09-13" version="2">MWLLALCLVGLAGAQRGGGGPGGGAPGGPSLGLGSLGEERFPVVNTAYGRVRGVRRELNNEILGPVVQFLGVPYATPPLGARRFQPPEAPASWPGVRNATTLPPACPQNLHGALPAIMLPVWFTDNLEAAATYVQNQSEDCLYLNLYVPTEDGPLTKKRDEATLNPPDTDIRDSGKKPVMLFLHGGSYMEGTGNMFDGSVLAAYGNVIVATLNYRLGVLGFLSTGDQAAKGNYGLLDQIQALRWLSENIAHFGGDPERITIFGSGAGASCVNLLILSHHSEGTSNLLSPAGCEQRGNHEQVMRPRPLLPSYHIAFGPVVDGDVVPDDPEILMQQGEFLNYDMLIGVNQGEGLKFVEDSAESEDGVSASAFDFTVSNFVDNLYGYPEGKDVLRETIKFMYTDWADRDNGEMRRKTLLALFTDHQWVAPAVATAKLHADYQSPVYFYTFYHHCQAEGRPEWADAAHGDELPYVFGVPMVGATDLFPCNFSKNDVMLSAVVMTYWTNFAKTGDPNQPVPQDTKFIHTKPNRFEEVVWSKFNSKEKQYLHIGLKPRVRDNYRANKVAFWLELVPHLHNLHTELFTTTTRLPPYATRWPPRPPPGAPGTRRPPPPATLPPEPEPEPGPRAYDRFPGDSRDYSTELSVTVAVGASLLFLNILAFAALYYKRDRRQELRCRRLSPPGGSGSGVPGGGPLLPATGRELPPEEELVSLQLKRGGGVGADPAEALRPACPPDYTLALRRAPDDVPLLAPGALTLLPSGLGPPPPPPPPSLHPFGPFPPPPSSAPSHNNTLPHPHSTTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MMH9</accession>
    <name>A0A8C4MMH9_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">CD19 molecule</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">CD19</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MMH9"/>
    <dbReference type="Ensembl" id="ENSEAST00005027456.2">
        <property type="protein sequence ID" value="ENSEASP00005025288.2"/>
        <property type="gene ID" value="ENSEASG00005017213.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014991"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
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        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002322">
        <property type="term" value="P:B cell proliferation involved in immune response"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:B cell receptor signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001923">
        <property type="term" value="P:B-1 B cell differentiation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016064">
        <property type="term" value="P:immunoglobulin mediated immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051281">
        <property type="term" value="P:positive regulation of release of sequestered calcium ion into cytosol"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050864">
        <property type="term" value="P:regulation of B cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050855">
        <property type="term" value="P:regulation of B cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001701">
        <property type="entry name" value="CD19 molecule"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042341">
        <property type="entry name" value="CD19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16674">
        <property type="entry name" value="B-LYMPHOCYTE ANTIGEN CD19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16674:SF2">
        <property type="entry name" value="B-LYMPHOCYTE ANTIGEN CD19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040412752" evidence="3">
        <location>
            <begin position="21"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="299"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="4">
        <location>
            <begin position="172"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="331"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="478"/>
            <end position="521"/>
        </location>
    </feature>
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        <location>
            <begin position="135"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="354"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
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            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025288.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="521" mass="57227" checksum="957B8EBCB3242220" modified="2023-09-13" version="2">MPPPLLLFFLLFLTPTGGRSQEALLVETKEGDNAVLPCFSPSDGPPQKLARYKEAEAFLQLSRKLRDLSIQKRPLGIMLLVFNVSNQMGGFYLCQPGPPSEQGWQPGWTVSVEGSGKLFRWNASDLDNPGCGLGKSSSEGPKTSSGHPASSQLYEWAKDQPHIRETALECAPNRSSLNQNHSQDLTVAPGSTLWLPCGVLPDSMVTGPVSWIHMHPEKTASVLHLNMTKDTQVREMWVSGTLRGGAVLLLPQATAQDAGTYHCYHGNTTREVHLKVTARSAVWHWLMETGGWKVPAATLVYLIFCLSSLVGFLHLRRALILRRKRKRMTDPTRRFFKVTPPPGTGAQNQYGNVLSLSTSSSGTGPEEEEGEAYEEPDSEEDSEFYENDSNLGQDQLSQDGSGYENPEEGALGAEDEDSFSNAAESYENEDEEVAQPVTRTTDFLSPHGSAWDPSREATSLGSQSYEDMRGILYAAPQLRSFRAQPGPNHEEDADSYENMDNPDGPEPAWDGGGHVGTWSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MPT6</accession>
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        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">TEP1</name>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the telomerase ribonucleoprotein complex that is essential for the replication of chromosome termini. Also a component of the ribonucleoprotein vaults particle, a multi-subunit structure involved in nucleo-cytoplasmic transport. Responsible for the localizing and stabilizing vault RNA (vRNA) association in the vault ribonucleoprotein particle. Binds to TERC.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Associated component of the telomerase holoenzyme complex. Component of the vault ribonucleoprotein particle, at least composed of MVP, PARP4 and one or more vault RNAs (vRNAs). Binds to VAULTRC1, VAULTRC2 and VAULTRC4/hvg4 vRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005027816.2">
        <property type="protein sequence ID" value="ENSEASP00005025618.2"/>
        <property type="gene ID" value="ENSEASG00005017468.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161338"/>
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        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005737">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005697">
        <property type="term" value="C:telomerase holoenzyme complex"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
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    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056829">
        <property type="entry name" value="Beta-prop_TEP1_2nd"/>
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    <dbReference type="InterPro" id="IPR056828">
        <property type="entry name" value="Beta-prop_TEP1_C"/>
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    <dbReference type="InterPro" id="IPR025139">
        <property type="entry name" value="DUF4062"/>
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    <dbReference type="InterPro" id="IPR045804">
        <property type="entry name" value="DUF5920"/>
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    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR052652">
        <property type="entry name" value="Telomerase_Complex_Comp"/>
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    <dbReference type="InterPro" id="IPR008850">
        <property type="entry name" value="TEP1_N"/>
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    <dbReference type="InterPro" id="IPR008858">
        <property type="entry name" value="TROVE_dom"/>
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    <dbReference type="InterPro" id="IPR037214">
        <property type="entry name" value="TROVE_dom_sf"/>
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    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
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    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
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    <dbReference type="PANTHER" id="PTHR44791:SF1">
        <property type="entry name" value="TELOMERASE PROTEIN COMPONENT 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR44791">
        <property type="entry name" value="TELOMERASE PROTEIN COMPONENT 1 TEP1"/>
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    <dbReference type="Pfam" id="PF25047">
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    <dbReference type="Pfam" id="PF25048">
        <property type="entry name" value="Beta-prop_TEP1_C"/>
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    <dbReference type="Pfam" id="PF13271">
        <property type="entry name" value="DUF4062"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19334">
        <property type="entry name" value="DUF5920"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05386">
        <property type="entry name" value="TEP1_N"/>
        <property type="match status" value="4"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140864">
        <property type="entry name" value="TROVE domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51226">
        <property type="entry name" value="TEP1_N"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50988">
        <property type="entry name" value="TROVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="9" id="KW-0779">Telomere</keyword>
    <keyword evidence="4 18" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="TROVE" evidence="21">
        <location>
            <begin position="228"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="20">
        <location>
            <begin position="1173"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="18">
        <location>
            <begin position="1766"/>
            <end position="1807"/>
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    </feature>
    <feature type="repeat" description="WD" evidence="18">
        <location>
            <begin position="1891"/>
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    <feature type="repeat" description="WD" evidence="18">
        <location>
            <begin position="2068"/>
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    <feature type="repeat" description="WD" evidence="18">
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            <begin position="2114"/>
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            <begin position="2155"/>
            <end position="2185"/>
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        <location>
            <begin position="2245"/>
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            <begin position="2287"/>
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    </feature>
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        <location>
            <begin position="2553"/>
            <end position="2583"/>
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    <feature type="region of interest" description="Disordered" evidence="19">
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            <begin position="387"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="875"/>
            <end position="899"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
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            <begin position="387"/>
            <end position="402"/>
        </location>
    </feature>
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        </source>
    </evidence>
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    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
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    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
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            <dbReference type="PROSITE" id="PS50988"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025618.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="2629" mass="290618" checksum="2ED874B67724AE2D" modified="2023-09-13" version="2">MEKLHEHVSAHPDILSLENRCLATLPDLKTMEKPHGHVSAHPNILSLENRCLSTLPDLKTVEKPHGHVSSHPNVLSLENRCLATLPDLKTVEKPHGHVSAHPNVLSLENRCLATLPILKSTVSASALLQRLQTFHPVQADLCSLNTSNHLLSEPPTWRAQYLSEGLGLLTFPGALQSISATETVQEAAVGHWSSSEEKRPGAEEWAEAPMPFYSLSFGEEEEVEELALKLTSGESESCPEPTDRALQEKKMALMSLLCSTRASNVNMKDAADPTPASLFEICSELAPLEPEFILKASLYTRQQLNVRDVANKVLAIAAFLPVCRPYLRRYFCAIVQLPSDWIQVAEFYQSLAEGEENKLVPLPSCLRAAMTDKFAQFDEYQLAKYNPRKHRAKSRPRRRPHPPKTEHPFSERQRYLPKCLAPLMDEQKKFEASYNAVPEKKKQPRFTLKKLVQRLHIKEPAQHVQALLGYRYPSNLQAFSRSHLPGPWDSSRAGKRMKLSQPETWERELSLRGNKASVWEELIDNGKLPFMAMLRNLCNLLRVGISPRHHELVLQRLQHAKSVIHSRQFPFRFLNAHDSISDLEAQLKNKALPFPSNTKLMRQIMIRNSKNIKRHAYQKKLCNPSRRELRAAMMIPLMYEQLKREKLKIHKSRQCKYDSETLARYRQALETAVNLSVKHSLPPLPGRTLLVYMTDAEADKLCPKNNPQGPPLNYVLLLIGMMIARAEHADLLLCGSGTLKTAVLKAEEGILKTAIELHAQVQELDGNYYEFSRDTFGKYLLSLAAQRVPVDRVILFGHIMNGSLINEAKQLFWQHVNSKCLFVGVCLRRICYTFSDSNPNDVTLLGCTDGILKFIAERGASRLLEHVGQMDKIFKIPPPPGKTGAPSLRPLEENTPSPLASISQSRWRKIRLFISSTFRDMHGERDLLLRSVLPALQTRAAPHRISLHAIDLRWGVTEEETRRNRQLEVCLGEVENSQLFVGILGSRYGYVPPNYNLPDHPHFRWAQQYPSGRSVTEMEVMQFLDRSLRLQPSAQALIYCRDSSFLRSVPDAWKSDFTSESEEAAHRISELKSSLNRQKGITCRRYACEWGGVAAGRPYVGGLEEFGQLVLQDVWNMIQKLYLQPEAQLEQPVSVPDDDLVQATFQQLRKPLSSARPRLLQDMVQQLMLHRGRLSLVTGQSGQGKTAFLASLVSALQAPDGVKVAPLVFFHFSGARPDQGLALTLLRRLCAYLHSQLQEPSALPSTYRGLVWHLQQRLLPKSAQSLQSGQTLVLIVDGADRLVDHHGELISDWIPKTLPRWVHLVLSMSTDAGLGESLERSQGAHVVALGPLEPSARARLVREELALYGKRLEESPFNNQMRLLLVKRGSALPLYLRLVTDHLRLFTLYEQVSERLRTLPATVPLLLQHILGTLEQEHGPNVLPQALATLEVTRSGLTVDQLHGVLSAWRILPMGTKTWEEAVAAGNSGDPYPMGPFAFLVQSLHSLLGEGPLERPGARLCLHDGPLRTAAKSRYGRRPGLEKTAHILIAAQLWKACDPDASGTFRSCPPEALGDLPYHLLQSENYGLLAKFLTNLHVMAAHLELGLISRLLEAHALYASSIPEEGQKLPEADVAVFHTFLRQQAPVLSQYPLLLPQQAANQPLDSPLCLQAPQLSQRWHLQRMLRWLNKPQTTRGQQTTSLSLAVSSSPTAVAFSPSGQRAAVGTASGTVYLLDLRTWQEEKSVVSGCDGVSSCSFLSDNALFLTAFDGLLELWDLQHGCRVLQTNAHQYQITSCCLSPDNRLLATVCLGGCLKLWDTVRGQLAFQHTCPKPLNCVAFHPEGQVVAIGSWSGSFSFLQVDGLKVTKELGAPGASVRTLAFNVPGQVVAVGRLDRMVELWAWKEGARLAAFPAHHGFVAAALFLRAGCQLLTAGEDGKVQVWSGSLGRPRGCLGSLSLSPALSVALSPDGDRVAVGYRADGIRIYKISSGSQGAQCQALDVAVSALAWLGPKVLVSGAEDGSLQGWALKESSLQSLWLLSRYQRPMLGLATSQELLASASEDFTVRLWPRQLLTLPQKAEDFPCATELRGHEGPVSCCSFSTDGGSLATGGRDRNLLCWDVRTPKAPVLICSFSACHRDWVTGCAWTKDNLLVSCSSDGSVGLWDPESGQQLGQFLGHQSAVSAVVAVEEHVVSVGRDGTLKVWDHQGVELTSIPAHSGPISQCAAALEPRAAGQPGSELLVVTAGLDGTTQLWHPLLVFQTHTLLGHSGPVSAAAVSETSGLLLTTSEDGSVRLWQVPKEADDTYIPRSPTAITAVAWAPDGSLAVSGNQAGELTLWQEAKAVATAQAPGRISSLVWYSAHTLIVLSADEKVSEWQVEWQKDSTPGNFSLHLKQVLQEDLGFLTGMALAPDGHSLILAKADLHLLRMKPGDAPSEIWTSFGEHPIMLSTHQDYGVFILQPTASGALSVLRQKESGEFEETLDFCVNLENPSGTLTLVTQAKPESESSFLCASSDGMLWNLAKCTLEGQWTTDNIWQKEAKMPETQTPGTDPCIDTDVDSWPRTTDLKTQQRRKIHSGSVTALHVLPELLVTASKDRDVKLWERPSMQLLGLFRCEGAASCLEPWLGPDSTLQLAVGDMQGNVYFLSWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MRZ5</accession>
    <name>A0A8C4MRZ5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Polypeptide N-acetylgalactosaminyltransferase</fullName>
            <ecNumber evidence="19">2.4.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Protein-UDP acetylgalactosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">GALNT7</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Glycopeptide transferase involved in O-linked oligosaccharide biosynthesis, which catalyzes the transfer of an N-acetyl-D-galactosamine residue to an already glycosylated peptide. In contrast to other proteins of the family, it does not act as a peptide transferase that transfers GalNAc onto serine or threonine residue on the protein receptor, but instead requires the prior addition of a GalNAc on a peptide before adding additional GalNAc moieties. Some peptide transferase activity is however not excluded, considering that its appropriate peptide substrate may remain unidentified.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-seryl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23956"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12788"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:53604"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-threonyl-[protein] + UDP-N-acetyl-alpha-D-galactosamine = a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:52424"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:67138"/>
            <dbReference type="ChEBI" id="CHEBI:87075"/>
            <dbReference type="EC" id="2.4.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 19">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 19">Golgi apparatus membrane</location>
            <topology evidence="2 19">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="19"/>
    <dbReference type="RefSeq" id="XP_044622884.1">
        <property type="nucleotide sequence ID" value="XM_044766949.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MRZ5"/>
    <dbReference type="Ensembl" id="ENSEAST00005033474.2">
        <property type="protein sequence ID" value="ENSEASP00005030787.2"/>
        <property type="gene ID" value="ENSEASG00005020974.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831726"/>
    <dbReference type="CTD" id="51809"/>
    <dbReference type="GeneTree" id="ENSGT00940000158105"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004653">
        <property type="term" value="F:polypeptide N-acetylgalactosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23437">
        <property type="entry name" value="beta-trefoil_Ricin_GALNT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02510">
        <property type="entry name" value="pp-GalNAc-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000019">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000031">
        <property type="entry name" value="Polypeptide N-acetylgalactosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045885">
        <property type="entry name" value="GalNAc-T"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11675:SF68">
        <property type="entry name" value="N-ACETYLGALACTOSAMINYLTRANSFERASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00458">
        <property type="entry name" value="RICIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 19" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="12 19" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 19" id="KW-0430">Lectin</keyword>
    <keyword evidence="15 19" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="Ricin B lectin" evidence="21">
        <location>
            <begin position="534"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="30"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00050905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00052209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00053496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU361242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM00458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030787.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="657" mass="75677" checksum="E8329264E3877984" modified="2023-09-13" version="2">MRLKLGFILRSLLVVGSFLGLVVLWSSLSPRPDDPSPLSRMREERDVNNPMPNRGGNGLAPGDDRFKPVVPWPHVEGVEVDLESIRRKNKAKNEQERHAGGDNQKDIIQRQYLTFKPQTFTYRDPVLRPGILGNFEPKEPEPHGVVGGPGEKAKPLVLGPEFKHAVQASIKEFGFNMVASDMISLDRSVNDLRQEECKYWHYDENLLTSSVVIVFHNEGWSTLMRTVHSVIKRTPRKYLAEIVLIDDFSNKEHLKEKLDDYIKLWNGLVKVFRNERREGLIQARSIGAQKAKLGQVLIYLDAHCEVAVNWYAPLIAPISKDRTTCTVPLIDYIDGNDYSIEPQQGGDEDGFARGAWDWSLLWKRIPLSHKEKAKRKHRTEPYRSPAMAGGLFAIEREFFFELGLYDPGLQIWGGENFEISYKIWQCGGKLLFVPCSRVGHIYRLEGWQGNPPPLYVGSSPTLKNYVRVVEVWWDEYKDYFYASRPESKALPYGDISELKKFREDHNCKSFKWFMEEIAYDITSHYPLPPKNVDWGEIRGFETVYCIDSMGRTNGGFVELGPCHRMGGNQLFRINEANQLMQYDQCLTKGPDGSKVMITHCNLNEFKEWQYFKNLHRFTHIPSGKCLDRSEVLHQVFISNCDSSKMTQKWEINNIHSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MU21</accession>
    <name>A0A8C4MU21_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DENN domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">DENND3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Forms oligomers. Interacts with 6 of the 7 known isoforms of 14-3-3 proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MU21"/>
    <dbReference type="Ensembl" id="ENSEAST00005033704.2">
        <property type="protein sequence ID" value="ENSEASP00005031001.2"/>
        <property type="gene ID" value="ENSEASG00005021125.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155784"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008333">
        <property type="term" value="P:endosome to lysosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032483">
        <property type="term" value="P:regulation of Rab protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11500:FF:000008">
        <property type="entry name" value="DENN domain containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11500">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001194">
        <property type="entry name" value="cDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005112">
        <property type="entry name" value="dDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043153">
        <property type="entry name" value="DENN_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051696">
        <property type="entry name" value="DENN_Domain_GEFs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057977">
        <property type="entry name" value="TPR_DENND3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005113">
        <property type="entry name" value="uDENN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296:SF21">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12296">
        <property type="entry name" value="DENN DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03455">
        <property type="entry name" value="dDENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02141">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25570">
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</entry>
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    <name>A0A8C4PKK2_EQUAS</name>
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        <submittedName>
            <fullName evidence="8">SDS3 homolog, SIN3A corepressor complex component</fullName>
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        <name evidence="8" type="primary">SUDS3</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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                <person name="Zhao"/>
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                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
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                <person name="Gao"/>
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                <person name="Ju"/>
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                <person name="Li"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
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                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="8" key="3">
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                <consortium name="Ensembl"/>
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            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PKK2"/>
    <dbReference type="Ensembl" id="ENSEAST00005013632.2">
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        <property type="gene ID" value="ENSEASG00005008761.2"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
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    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0019899">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:histone deacetylase activity"/>
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        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013907">
        <property type="entry name" value="Sds3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21964">
        <property type="entry name" value="BREAST CANCER METASTASIS-SUPPRESSOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="305"/>
            <end position="331"/>
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    </feature>
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            <begin position="149"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
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            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="44"/>
            <end position="58"/>
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    </feature>
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        <location>
            <begin position="68"/>
            <end position="88"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="102"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="124"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="135"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="J."/>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
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                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
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                <person name="R."/>
                <person name="Hou"/>
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                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="J."/>
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                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the claudin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014686522.1">
        <property type="nucleotide sequence ID" value="XM_014831036.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PRN7"/>
    <dbReference type="Ensembl" id="ENSEAST00005027130.2">
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        <property type="gene ID" value="ENSEASG00005017001.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106824621"/>
    <dbReference type="KEGG" id="eai:106824621"/>
    <dbReference type="CTD" id="137075"/>
    <dbReference type="GeneTree" id="ENSGT00390000006975"/>
    <dbReference type="OrthoDB" id="8790791at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0796">Tight junction</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="221"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025001.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="291" mass="31255" checksum="D2A0ACE787A3307D" modified="2023-09-13" version="2">MRTPVAMTVGMVFAPCGLLLNLTSTLTPGWRLVKGFLNQPVDVVLYQGLWDMCREQSSRERECGQPDDWGYFAAEPVRVARGLMVTSLAITALGLLLASLGVRCWQEEPRSALAGLSGLVLFAAGLFSLIPVSWYNHVLGDRAVLPAPSSPVTVQVSYSLVLGYLGSCLLLLGGFSLALSFAPWCEERCRRKAPSGGQRRSSISTVHVDWPEPALTPAIKYYSGGQHRPRPADLGAAGPAKVGFPMPRPPPKAHSDPEEDTLDGEKAADSHSASSRSTRLCHGSLPCDSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PU01</accession>
    <name>A0A8C4PU01_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Zinc finger protein 134</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ZNF134</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PU01"/>
    <dbReference type="Ensembl" id="ENSEAST00005032542.2">
        <property type="protein sequence ID" value="ENSEASP00005029945.2"/>
        <property type="gene ID" value="ENSEASG00005020367.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154693"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002682">
        <property type="term" value="P:regulation of immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004935">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001478">
        <property type="entry name" value="Zinc finger protein 134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002072">
        <property type="entry name" value="Zinc finger protein 134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002402">
        <property type="entry name" value="Zinc finger protein 347"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000135">
        <property type="entry name" value="Zinc finger protein 358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000443">
        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001697">
        <property type="entry name" value="zinc finger protein 623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002863">
        <property type="entry name" value="Zinc finger protein 671"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000176">
        <property type="entry name" value="zinc finger protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF4">
        <property type="entry name" value="ZINC FINGER PROTEIN 134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="50"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="203"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="231"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="259"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="287"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="315"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="343"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="371"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="399"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="24"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005029945.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="426" mass="48513" checksum="84AE0D620A9F49C3" modified="2023-09-13" version="2">MTLATAGGAWTGFDCWRGVADEEAPSEQSISVGVSHVSTSKADSSTQMPHPCDICGPILKDILHLGEHQETHHGLKPYTCGACGRQFCFSENFHQHQKQYNVEKPLRRDKGKASCVKNCRVCEDPHLSEKPFTCEEEQKNFQATLGSHQQKATHSKRKKRSTESGEAFHIGQMHYKCSECGKAFNRKDTLVQHQRIHTGEKPYECSECGKAFSRKATLVQHQRIHTGERPYECSECGKAFSRKDNLTQHKRIHTGEMPYKCGECGKYFSHHSNLIVHQRVHNGARPYKCNDCGKVFRHKSTLVQHESIHTGENPYVCSDCGKSFGHKYTLIKHQRIHTEVRPFECVECGKFFSRSSDFIAHQRVHTGERPFVCSKCGKDFIRTSHLVRHQKVHTGERPYECNECGKAYSLSSHLIRHQKVHTAGRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PVF1</accession>
    <name>A0A8C4PVF1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">ATPase family AAA domain-containing protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ATAD2</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044599707.1">
        <property type="nucleotide sequence ID" value="XM_044743772.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044599709.1">
        <property type="nucleotide sequence ID" value="XM_044743774.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005035980.2">
        <property type="protein sequence ID" value="ENSEASP00005033009.2"/>
        <property type="gene ID" value="ENSEASG00005022524.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106848109"/>
    <dbReference type="KEGG" id="eai:106848109"/>
    <dbReference type="CTD" id="29028"/>
    <dbReference type="GeneTree" id="ENSGT00550000074694"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05528">
        <property type="entry name" value="Bromo_AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19517">
        <property type="entry name" value="RecA-like_Yta7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.920.10:FF:000021">
        <property type="entry name" value="ATPase family AAA domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000016">
        <property type="entry name" value="ATPase family AAA domain-containing protein 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000734">
        <property type="entry name" value="ATPase family, AAA domain containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000061">
        <property type="entry name" value="ATPase family, AAA domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045199">
        <property type="entry name" value="ATAD2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069:SF4">
        <property type="entry name" value="ATPASE FAMILY AAA DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 17" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Bromo" evidence="19">
        <location>
            <begin position="1053"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="265"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1175"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1226"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="293"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1179"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1193"/>
            <end position="1204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1229"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="1273"/>
            <end position="1292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00071858"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005033009.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1437" mass="163414" checksum="2373603F8110105B" modified="2023-09-13" version="2">MRRRDSGRARCGRWRGACGARIRSAEEPERLPSPARAHLPPRTAGRGGQKMVVLRSSLELHSHSAASATDSLDLSNEFLSLEQIGRRRLRSATAAGQSAVTAAAAGDGSSVKEVETYHRTRALRSLRKNAQNSSGSSFEKNMEITEKHANGRHFTRQLARQQADKKKEECKEDKVIPTTRSLRTRNIVQTTEHLHEENGDVEVRRSCRIRSRYSTVNQSVLFDKLITNTAEAVLQKMDDMKKMRRQRMRELEDLGVFNETEEGNLNMYTRGKQKDIQRTDEETTDNQEGSVESSEEGEDQEDEDDGEDEDDGEDEDDDEDDDDDEDEEDGEEDNQKRYYLRQRKATVYYQAPLEKPRHQRKPNIFYSGPASPARPRYRLSSAGPRSPYCKRMNRRRHAIHSSDSTSSSSSEDEQHFERRRKRSRNRAINRCLPLNFRKDELKGIYKDRMKIGASLADVDPMQLDSSVRFDSVGGLSNHIAALKEMVVFPLLYPEVFEKFKIQPPRGCLFYGPPGTGKTLVARALANECSQGDKRVAFFMRKGADCLSKWVGESERQLRLLFDQAYQMRPSIIFFDEIDGLAPVRSSRQDQIHSSIVSTLLALMDGLDSRGEIVVIGATNRLDSIDPALRRPGRFDREFLFSLPDKDARKEILKIHTRDWNPKPLDVFLEELAENCVGYCGADIKSICSEAALCALRRRYPQIYTTSEKLQLDLSSINISAKDFEVAMHKMIPASQRAVTSPGQALSTIVKPLLQGTVHKILEALQRVFPHAEIRTNKALDSDISCPLLESDLAYSDDDVSSVYENGLSQKSFNKPKENFNFLHLNRNACYQPMSFRPRILIVGEPGFGQGSHLAPAVIHALEKFAVYTLDIPVLFGVSATSPEETCAQMIREAKRTAPSIVYVPHIHLWWEIVGPTLKATFTTLLQNIPSFAPVLLLATSDKPHCALPEEVQELFIRDYGEIFNVQLPGKEERTKFFEDLILKQAAKPPISKKKAVLQALEVLPVAPPPEPRPLTAEEVKRLEEQEEDTFRELRIFLRNVTHRLAIDKRFRVFTKPVDPDEVPDYVTVIKQPMDLSSVISKIDLHKYLTVKDYLSDIDLICSNALEYNPDRDPGDRLIRHRACALRDTAYAIIKEELDEDFEQLCEEIQESRKKRGCSSSRYAPSYYHVMPKQNATAVGDKRSDPEQNEKLKTPSTPVACSTPAQLKRKIRKKSKWYLGTIKKRRRISQTKDDSQNVMSDKIESDTEENQDTSVDHNETGNTGESSMEENEKQQNASESKMELGNNTSSNCSIENELEESGKITACTELRKDKIVCNGDASSAQIIDTPDENEAKEMCVLRMTRARRSQVEQQQLISVEKALAILSQPTPSLVVDHERLKNLLKTVIKKSREYNIFQLENLYAVISQCIYQHRRDYDKTTLIQKMEQEVENFSCSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I6X8</accession>
    <name>A0A9L0I6X8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Metaxin-2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Mitochondrial outer membrane import complex protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">MTX2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in transport of proteins into the mitochondrion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with MTX1/metaxin-1. Associates with the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MICOS10/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and QIL1/MIC13. This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2 (together described as components of the mitochondrial outer membrane sorting assembly machinery (SAM) complex) and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9. The MICOS and SAM complexes together with DNAJC11 are part of a large protein complex spanning both membranes termed the mitochondrial intermembrane space bridging (MIB) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the metaxin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0I6X8"/>
    <dbReference type="Ensembl" id="ENSEAST00005076510.1">
        <property type="protein sequence ID" value="ENSEASP00005036094.1"/>
        <property type="gene ID" value="ENSEASG00005022537.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182919"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001401">
        <property type="term" value="C:SAM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006839">
        <property type="term" value="P:mitochondrial transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03211">
        <property type="entry name" value="GST_C_Metaxin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03079">
        <property type="entry name" value="GST_N_Metaxin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1050.10:FF:000033">
        <property type="entry name" value="metaxin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036282">
        <property type="entry name" value="Glutathione-S-Trfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040079">
        <property type="entry name" value="Glutathione_S-Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033468">
        <property type="entry name" value="Metaxin_GST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050931">
        <property type="entry name" value="Mito_Protein_Transport_Metaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019564">
        <property type="entry name" value="Sam37/metaxin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289">
        <property type="entry name" value="METAXIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12289:SF38">
        <property type="entry name" value="METAXIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17171">
        <property type="entry name" value="GST_C_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10568">
        <property type="entry name" value="Tom37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00019">
        <property type="entry name" value="Glutathione_Transferase_(cytos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01180">
        <property type="entry name" value="SUF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47616">
        <property type="entry name" value="GST C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Mitochondrial outer membrane transport complex Sam37/metaxin N-terminal" evidence="13">
        <location>
            <begin position="66"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Metaxin glutathione S-transferase" evidence="14">
        <location>
            <begin position="212"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00063864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00072180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00076977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF10568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF17171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005036094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="292" mass="32886" checksum="3085FA8E10A59DD0" modified="2023-09-13" version="1">MSLVAEAFVSQIAVRWAKVNVIISSTDKGMGKSYPQTIAAEPWPENATLYQQLKGEQILLSDNAASLAVQAFLQMCNLPIKVVCRANAEYMSPSGKVPFIHVGNQVVSELGPIVQFVKAKGHSLSDGLDEVQKAEMKAYMELVNNMLLTAELYLQWCDEATVGEITHARYGSPYPWPLNHILAYQKQWEVRRKMKAIGWGKKTLDQVLEDVDQCCQALSQRLGTQPYFFNKQPTELDALVFGHLYTILTTQLTSDELSEKVKNYSNLLAFCRRIEQHYFEDRGKGSSSIRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I850</accession>
    <name>A0A9L0I850_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="22">Gamma-aminobutyric acid type A receptor subunit beta2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="22" type="primary">GABRB2</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>chloride(in) = chloride(out)</text>
            <dbReference type="Rhea" id="RHEA:29823"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="18">Postsynaptic cell membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the ligand-gated ion channel (TC 1.A.9) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044633641.1">
        <property type="nucleotide sequence ID" value="XM_044777706.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0I850"/>
    <dbReference type="Ensembl" id="ENSEAST00005055273.1">
        <property type="protein sequence ID" value="ENSEASP00005036677.1"/>
        <property type="gene ID" value="ENSEASG00005000588.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106832152"/>
    <dbReference type="KEGG" id="eai:106832152"/>
    <dbReference type="CTD" id="2561"/>
    <dbReference type="GeneTree" id="ENSGT00940000154245"/>
    <dbReference type="OMA" id="INKMDPH"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902711">
        <property type="term" value="C:GABA-A receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005230">
        <property type="term" value="F:extracellular ligand-gated monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004890">
        <property type="term" value="F:GABA-A receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18999">
        <property type="entry name" value="LGIC_ECD_GABAAR_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19053">
        <property type="entry name" value="LGIC_TM_GABAAR_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.390:FF:000004">
        <property type="entry name" value="Gamma-aminobutyric acid receptor subunit beta-2 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.170.10:FF:000004">
        <property type="entry name" value="Gamma-aminobutyric acid receptor subunit beta-2 isoform A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.170.10">
        <property type="entry name" value="Neurotransmitter-gated ion-channel ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.390">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006028">
        <property type="entry name" value="GABAA/Glycine_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002289">
        <property type="entry name" value="GABAAb_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006202">
        <property type="entry name" value="Neur_chan_lig-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036734">
        <property type="entry name" value="Neur_chan_lig-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006201">
        <property type="entry name" value="Neur_channel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036719">
        <property type="entry name" value="Neuro-gated_channel_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038050">
        <property type="entry name" value="Neuro_actylchol_rec"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006029">
        <property type="entry name" value="Neurotrans-gated_channel_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018000">
        <property type="entry name" value="Neurotransmitter_ion_chnl_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00860">
        <property type="entry name" value="LIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18945">
        <property type="entry name" value="NEUROTRANSMITTER GATED ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02931">
        <property type="entry name" value="Neur_chan_LBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02932">
        <property type="entry name" value="Neur_chan_memb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01160">
        <property type="entry name" value="GABAARBETA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00253">
        <property type="entry name" value="GABAARECEPTR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00252">
        <property type="entry name" value="NRIONCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90112">
        <property type="entry name" value="Neurotransmitter-gated ion-channel transmembrane pore"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63712">
        <property type="entry name" value="Nicotinic receptor ligand binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="5">Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process.</text>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="3" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <dbReference type="RefSeq" id="XP_014717571.1">
        <property type="nucleotide sequence ID" value="XM_014862085.3"/>
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        <property type="component" value="Chromosome 13"/>
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        <property type="term" value="C:centriolar satellite"/>
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        <property type="term" value="C:ciliary basal body"/>
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        <property type="term" value="C:microtubule plus-end"/>
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        <property type="term" value="C:mitotic spindle pole"/>
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        <property type="term" value="F:microtubule binding"/>
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        <property type="term" value="P:cell division"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:establishment of spindle orientation"/>
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        <property type="term" value="P:positive regulation of intracellular transport"/>
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    <dbReference type="GO" id="GO:0051988">
        <property type="term" value="P:regulation of attachment of spindle microtubules to kinetochore"/>
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    <dbReference type="GO" id="GO:0090235">
        <property type="term" value="P:regulation of metaphase plate congression"/>
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    <dbReference type="GO" id="GO:0007051">
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="Astrin"/>
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    <dbReference type="PANTHER" id="PTHR15347">
        <property type="entry name" value="SPERM-ASSOCIATED ANTIGEN 5"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR15347:SF1">
        <property type="entry name" value="SPERM-ASSOCIATED ANTIGEN 5"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="60"/>
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            <begin position="465"/>
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            <begin position="553"/>
            <end position="580"/>
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    <feature type="coiled-coil region" evidence="1">
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            <begin position="697"/>
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        <location>
            <begin position="1012"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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            <begin position="398"/>
            <end position="408"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="414"/>
            <end position="423"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
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            <begin position="470"/>
            <end position="479"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
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            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
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<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IE02</accession>
    <name>A0A9L0IE02_EQUAS</name>
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        <recommendedName>
            <fullName evidence="27">Amine oxidase</fullName>
            <ecNumber evidence="27">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
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        <name evidence="30" type="primary">LOC106838326</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes the oxidative deamination of primary and some secondary amine such as neurotransmitters, with concomitant reduction of oxygen to hydrogen peroxide and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. Preferentially oxidizes serotonin. Also catalyzes the oxidative deamination of kynuramine to 3-(2-aminophenyl)-3-oxopropanal that can spontaneously condense to 4-hydroxyquinoline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>(R)-adrenaline + O2 + H2O = (R)-3,4-dihydroxymandelaldehyde + methylamine + H2O2</text>
            <dbReference type="Rhea" id="RHEA:51168"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:59338"/>
            <dbReference type="ChEBI" id="CHEBI:71406"/>
            <dbReference type="ChEBI" id="CHEBI:180943"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(R)-noradrenaline + O2 + H2O = (R)-3,4-dihydroxymandelaldehyde + H2O2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:69076"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:72587"/>
            <dbReference type="ChEBI" id="CHEBI:180943"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>2-phenylethylamine + O2 + H2O = 2-phenylacetaldehyde + H2O2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:25265"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16424"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:225237"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>a primary methyl amine + O2 + H2O = an aldehyde + H2O2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16153"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:228804"/>
            <dbReference type="EC" id="1.4.3.21"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a secondary aliphatic amine + O2 + H2O = a primary amine + an aldehyde + H2O2</text>
            <dbReference type="Rhea" id="RHEA:26414"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:17478"/>
            <dbReference type="ChEBI" id="CHEBI:58855"/>
            <dbReference type="ChEBI" id="CHEBI:65296"/>
            <dbReference type="EC" id="1.4.3.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>dopamine + O2 + H2O = 3,4-dihydroxyphenylacetaldehyde + H2O2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:27946"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:27978"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:59905"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>kynuramine + O2 + H2O = 3-(2-aminophenyl)-3-oxopropanal + H2O2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:59596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:180898"/>
            <dbReference type="ChEBI" id="CHEBI:180899"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:59597"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>serotonin + O2 + H2O = (5-hydroxyindol-3-yl)acetaldehyde + H2O2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:69072"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:50157"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tryptamine + O2 + H2O = indole-3-acetaldehyde + H2O2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:59416"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:18086"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57887"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>tyramine + O2 + H2O = (4-hydroxyphenyl)acetaldehyde + H2O2 + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:30591"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:15621"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:327995"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 27">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Monomer, homo- or heterodimer (containing two subunits of similar size). Each subunit contains a covalently bound flavin. Enzymatically active as monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion outer membrane</location>
            <topology evidence="2">Single-pass type IV membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 27">Belongs to the flavin monoamine oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="27"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0IE02"/>
    <dbReference type="Ensembl" id="ENSEAST00005080092.1">
        <property type="protein sequence ID" value="ENSEASP00005038436.1"/>
        <property type="gene ID" value="ENSEASG00005020177.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160514"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097621">
        <property type="term" value="F:monoamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006584">
        <property type="term" value="P:catecholamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.405.10:FF:000005">
        <property type="entry name" value="Amine oxidase [flavin-containing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002937">
        <property type="entry name" value="Amino_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001613">
        <property type="entry name" value="Flavin_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050703">
        <property type="entry name" value="Flavin_MAO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43563">
        <property type="entry name" value="AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43563:SF11">
        <property type="entry name" value="AMINE OXIDASE [FLAVIN-CONTAINING] A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01593">
        <property type="entry name" value="Amino_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00757">
        <property type="entry name" value="AMINEOXDASEF"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0128">Catecholamine metabolism</keyword>
    <keyword evidence="7 27" id="KW-0274">FAD</keyword>
    <keyword evidence="4 27" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="8" id="KW-0531">Neurotransmitter degradation</keyword>
    <keyword evidence="11 27" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 27" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="925"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="domain" description="Amine oxidase" evidence="29">
        <location>
            <begin position="448"/>
            <end position="885"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="73"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="124"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="28">
        <location>
            <begin position="356"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="28">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="28">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="28">
        <location>
            <begin position="141"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="28">
        <location>
            <begin position="366"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="28">
        <location>
            <begin position="405"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="449"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <begin position="468"/>
            <end position="469"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="669"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="777"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="26">
        <location>
            <position position="861"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022867"/>
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            <dbReference type="ARBA" id="ARBA00022939"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023128"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00047794"/>
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            <dbReference type="ARBA" id="ARBA00048448"/>
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            <dbReference type="ARBA" id="ARBA00048463"/>
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            <dbReference type="ARBA" id="ARBA00048466"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU362067"/>
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    </evidence>
    <evidence type="ECO:0000256" key="28">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Pfam" id="PF01593"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038436.1"/>
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    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IEA3</accession>
    <name>A0A9L0IEA3_EQUAS</name>
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        <submittedName>
            <fullName evidence="18">Lysophosphatidylcholine acyltransferase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LPCAT1</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; phospholipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="15">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IEA3"/>
    <dbReference type="Ensembl" id="ENSEAST00005079089.1">
        <property type="protein sequence ID" value="ENSEASP00005039255.1"/>
        <property type="gene ID" value="ENSEASG00005032107.1"/>
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    <dbReference type="GeneTree" id="ENSGT01030000234574"/>
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        <property type="component" value="Chromosome 10"/>
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    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047184">
        <property type="term" value="F:1-acylglycerophosphocholine O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042171">
        <property type="term" value="F:lysophosphatidic acid acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07991">
        <property type="entry name" value="LPLAT_LPCAT1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045252">
        <property type="entry name" value="LPCAT1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF57">
        <property type="entry name" value="LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063">
        <property type="entry name" value="PHOSPHOLIPID ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01553">
        <property type="entry name" value="Acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="4" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="13" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="EF-hand" evidence="17">
        <location>
            <begin position="331"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="441"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="475"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="486" mass="54260" checksum="B89DF2D8BE01ADDE" modified="2023-09-13" version="1">MTLTLFPLRLLVATFMMLLAWPIVLVASLGSAEQEPTQPPALWRRVVDFLLRAILRTMWFAGGFHRVIVKGRQALPTEAAILTLAPHSSYFDAIPITMTMSSIVMKAESRDIPIWGTLMQHIRPLLVSRSDKDSRRKTVEEIKRRAQSNGKWPQIMIFPEGTCTNRTCLITFKPGAFIPGVPVQPVVLRYPNKLDTITWTWQGPGALKILWLTLCQFHNQVEIEFLPVYSPSEAEKKDPVLYASNVRRVMAAALGVPVTDYTYEDCQLALAEGQLRLPADTCLLEFARLVRGLGLKPEKLEQDLDRYAESAKTRRGEKIRLAEFAAFLQAPASAAVEDLFSLFDESGGGKMDLREYVVALSVVCRPSQTLDTMQLAFKMYGSEEDGSIDEDALSSILKTALGVAELTVTDLFRAIDQEEKEKITFADFNRLAETSPNFAEEYLYPSQTRPDSPGQPPPAPAPNGFCVDFSPKSSDAGEKPFHKKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IFA4</accession>
    <name>A0A9L0IFA4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ARF interacting protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ARFIP2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014707602.1">
        <property type="nucleotide sequence ID" value="XM_014852116.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IFA4"/>
    <dbReference type="SMR" id="A0A9L0IFA4"/>
    <dbReference type="Ensembl" id="ENSEAST00005081786.1">
        <property type="protein sequence ID" value="ENSEASP00005036450.1"/>
        <property type="gene ID" value="ENSEASG00005001702.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106838182"/>
    <dbReference type="KEGG" id="eai:106838182"/>
    <dbReference type="CTD" id="23647"/>
    <dbReference type="GeneTree" id="ENSGT00950000183040"/>
    <dbReference type="OrthoDB" id="9994780at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034315">
        <property type="term" value="P:regulation of Arp2/3 complex-mediated actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07660">
        <property type="entry name" value="BAR_Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000003">
        <property type="entry name" value="arfaptin-2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010504">
        <property type="entry name" value="AH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030798">
        <property type="entry name" value="Arfaptin_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141:SF3">
        <property type="entry name" value="ARFAPTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12141">
        <property type="entry name" value="ARFAPTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06456">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01015">
        <property type="entry name" value="Arfaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50870">
        <property type="entry name" value="AH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AH" evidence="6">
        <location>
            <begin position="121"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="46"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005036450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="341" mass="37711" checksum="04085CA06F349160" modified="2023-09-13" version="1">MTDGILGKAATMEIPIHGNGEAGQLPEDDGLEQDLQQVMVSGPNLNETSIVSGGYGGSGDGLIPTGSGRHPSHSATPAGPGDEVARGIAGEKFDIVKKWGINTYKCTKQLLSERFGRGSRTVDLELELQIELLRETKRKYESVLQLGRALTAHLYSLLQTQHALGDAFADLSQKSPELQEEFGYNAETQKLLCKNGETLLGAVNFFVSSINTLVTKTMEDTLMTVKQYEAARLEYDAYRTDLEELSLGPRDAGTRGRLESAQATFQAHRDKYEKLRGDVAIKLKFLEENKIKVMHKQLLLFHNAVSAYFAGNQKQLEQTLQQFNIKLRPPGAEKPSWLEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IFG0</accession>
    <name>A0A9L0IFG0_EQUAS</name>
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        <recommendedName>
            <fullName evidence="12">Myoneurin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">MYNN</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
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    <dbReference type="Ensembl" id="ENSEAST00005061605.1">
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        <property type="term" value="P:cellular response to leukemia inhibitory factor"/>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="R."/>
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                <person name="Q."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the CFAP298 family.</text>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="PROTEIN C21ORF59"/>
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    </dbReference>
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            <dbReference type="ARBA" id="ARBA00009619"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <sequence length="297" mass="33951" checksum="0F343323D80F67E7" modified="2023-09-13" version="1">MVLLHVKRGDESQFLLQAPGSTELEELTVQVARVYNARLKVQRVCSEMEELAEHGVFLPPNMQGLTDDQIEELKLRDEWGEKCVPSGGSVFKKDDIGRRNGQAPNEKMKQVLKKTVEEAKAIISKKQVEAGVCVTAEMVKDALDQLRGAVMIVYPMGLPPYDPVRMEFENKEDLSGTQRSLPWPQAGLSVIEESEAQLWWAAKELRRTKKLSDYVGKNEKTKIIVKIQQRGQGAPAREPIISSEEQKQLMLHYHRRQEELKKLEENDDDSCLNSPWADNTALKRHFHGVKDIKWRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IG62</accession>
    <name>A0A9L0IG62_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Mitochondrial fission factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">MFF</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Plays a role in mitochondrial and peroxisomal fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. May be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. Interacts with DNM1L. Interacts with C11orf65/MFI; the interaction inhibits MFF interaction with DNM1L.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Mitochondrion outer membrane</location>
            <topology evidence="12">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the Tango11 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070345724.1">
        <property type="nucleotide sequence ID" value="XM_070489623.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070345725.1">
        <property type="nucleotide sequence ID" value="XM_070489624.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070345726.1">
        <property type="nucleotide sequence ID" value="XM_070489625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IG62"/>
    <dbReference type="Ensembl" id="ENSEAST00005050359.1">
        <property type="protein sequence ID" value="ENSEASP00005039925.1"/>
        <property type="gene ID" value="ENSEASG00005008270.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106827861"/>
    <dbReference type="CTD" id="56947"/>
    <dbReference type="GeneTree" id="ENSGT00390000009776"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090141">
        <property type="term" value="P:positive regulation of mitochondrial fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090314">
        <property type="term" value="P:positive regulation of protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039433">
        <property type="entry name" value="Mff-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008518">
        <property type="entry name" value="Mff/Tango-11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16501:SF17">
        <property type="entry name" value="MITOCHONDRIAL FISSION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16501">
        <property type="entry name" value="TRANSPORT AND GOLGI ORGANIZATION PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05644">
        <property type="entry name" value="Miff"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 12" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="10 12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="Mff-like" evidence="14">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="Mff-like" evidence="14">
        <location>
            <begin position="148"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="169"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU368040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF05644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="218" mass="25052" checksum="3A0A90B0CA075D93" modified="2023-09-13" version="1">MAEISRIQYEMEYTEGISQRMRVPEKLKVAPPNADLEQGFQEGVPNASVVMQVPERIVVAGNNEDIPFSRPADLDLIQATPFKPLALKTPPRVLTLSERPLDFLDLERPPPTPQNEEIRAVGRLKRERSMSENAVRQNGQLVRTDSMYGISNADTTLEATSDDMTVVDAASLRRQIIKLNRRLQLLEEENKERAKREMVMYSITVAFWLLNSWLWFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IGF9</accession>
    <name>A0A9L0IGF9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Small RNA 2'-O-methyltransferase</fullName>
            <ecNumber evidence="12">2.1.1.386</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">HEN1 methyltransferase homolog 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">HENMT1</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Methyltransferase that adds a 2'-O-methyl group at the 3'-end of piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. This probably protects the 3'-end of piRNAs from uridylation activity and subsequent degradation. Stabilization of piRNAs is essential for gametogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>small RNA 3'-end nucleotide + S-adenosyl-L-methionine = small RNA 3'-end 2'-O-methylnucleotide + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:37887"/>
            <dbReference type="Rhea" id="RHEA-COMP:10415"/>
            <dbReference type="Rhea" id="RHEA-COMP:10416"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:74898"/>
            <dbReference type="EC" id="2.1.1.386"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyltransferase superfamily. HEN1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.386" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0IGF9"/>
    <dbReference type="Ensembl" id="ENSEAST00005056616.1">
        <property type="protein sequence ID" value="ENSEASP00005039973.1"/>
        <property type="gene ID" value="ENSEASG00005000165.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004798"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090486">
        <property type="term" value="F:small RNA 2'-O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034587">
        <property type="term" value="P:piRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001510">
        <property type="term" value="P:RNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000124">
        <property type="entry name" value="HEN methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026610">
        <property type="entry name" value="Hen1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21404">
        <property type="entry name" value="HEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21404:SF3">
        <property type="entry name" value="SMALL RNA 2'-O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="10" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00053983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="422" mass="48021" checksum="AC71D4C3F166F06C" modified="2023-09-13" version="1">MAENKTEFQCSGVVGGDFEAVRKKGIEFDPPLYKQRYFFVKDLVNQHKTKKVADLGCGDNTLLWILKIHSCAELLVGVDINDSVLHYSRNKLSPSWGDQLSPRDLDLTVTLYIGSAVERDSRLRGFDLITCIELIEHLNSEDLARFPEVVFGYFSPSMIVISTPNSEFNPLFPSGNALRDLDHKFEWNRMQFQTWALDVANRYNYSVEFTGVGEPPAGAENVGYCTQIGIFRKNEAKATKLCISEQDDEHVYELFYTELYPSLQQEKVRKFVVLNEVYRQVYLMRRRFIKILDPLAASIFNSADCDPAKVEERRLLGPPLFTDARLAEIRLLGPPFTEAEKTEFILLGPAFTEAEKTKIVNSPKPFCVGNEFCVPLERLLAYPKVNRICDSIETLKMYIADTVTLSSDGSAMKVDIPDHDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IGL3</accession>
    <name>A0A9L0IGL3_EQUAS</name>
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        <recommendedName>
            <fullName evidence="4 15">Zinc finger MYND domain-containing protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ZMYND10</name>
    </gene>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13 15">Plays a role in axonemal structure organization and motility. Involved in axonemal pre-assembly of inner and outer dynein arms (IDA and ODA, respectively) for proper axoneme building for cilia motility. May act by indirectly regulating transcription of dynein proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts (via C-terminus) with DNAAF11 (via CS domain); this interaction stabilizes DNAAF11 at the protein level. Interacts (via C-terminus) with DNAL1; this interaction stabilizes DNAL1 at the protein level. Interacts with DNAAF4, HSPA8, IQUB, RUVBL2 and DYNTL5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Apical cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
            <location evidence="2">Centriolar satellite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Dynein axonemal particle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the ZMYND10 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IGL3"/>
    <dbReference type="Ensembl" id="ENSEAST00005070961.1">
        <property type="protein sequence ID" value="ENSEASP00005040023.1"/>
        <property type="gene ID" value="ENSEASG00005012147.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106830733"/>
    <dbReference type="CTD" id="51364"/>
    <dbReference type="GeneTree" id="ENSGT00940000153820"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034451">
        <property type="term" value="C:centriolar satellite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120293">
        <property type="term" value="C:dynein axonemal particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003341">
        <property type="term" value="P:cilium movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036159">
        <property type="term" value="P:inner dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044458">
        <property type="term" value="P:motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036158">
        <property type="term" value="P:outer dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905505">
        <property type="term" value="P:positive regulation of motile cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061512">
        <property type="term" value="P:protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="6.10.140.2220:FF:000009">
        <property type="entry name" value="Zinc finger MYND domain-containing protein 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.2220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017333">
        <property type="entry name" value="UCP037948_Znf-MYND"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052298">
        <property type="entry name" value="ZMYND10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002893">
        <property type="entry name" value="Znf_MYND"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13244">
        <property type="entry name" value="ZINC FINGER MYND DOMAIN CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13244:SF7">
        <property type="entry name" value="ZINC FINGER MYND DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01753">
        <property type="entry name" value="zf-MYND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037948">
        <property type="entry name" value="UCP037948_Znf_MYND10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01360">
        <property type="entry name" value="ZF_MYND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50865">
        <property type="entry name" value="ZF_MYND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYND-type" evidence="17">
        <location>
            <begin position="394"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00024190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRNR" id="PIRNR037948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005040023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="440" mass="50450" checksum="A988E2649B408F24" modified="2023-09-13" version="1">MGDLELLLPGEADVLVRGLRSFPLREMGSGGWNQQHENLEKLNMQAILDATASQGEPIQELLVTHGKIPVLVEELIAVEMWKQKVFPVLCRLEDFKPLNTFPIYMVVHHEASIINLLETVFFHKEVCESAEDTVLDLVDYCHRKLTLLVARSGDSGPPEDDESRYSTPMQELQKQAELMEFEIALKALSVLRYITDCVDSLSLSTLSRMLSTHNLPCLLVELLEHSPWSRREGGKLQQFEGSRWQTVAPSEQQKLSKLDGQVWIALYNLLLSPETRARYCLTSFAKGQLLKLRAFLTDTLLDQLPNLADLQSFLTQLALAEPQPPKKDLILEQIPEIWERLERENRGKWQAIAKYQLRHVFSPSEQDLRLQARRWAETYRLDVLEAVAPERPRCAYCSAEASKRCSRCQNEWYCCRECQVKHWEKHGKACVLAAQGDRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IHY3</accession>
    <name>A0A9L0IHY3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Leukotriene A(4) hydrolase</fullName>
            <shortName evidence="16">LTA-4 hydrolase</shortName>
            <ecNumber evidence="16">3.3.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">LTA4H</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>leukotriene A4 + H2O = leukotriene B4</text>
            <dbReference type="Rhea" id="RHEA:22324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57461"/>
            <dbReference type="ChEBI" id="CHEBI:57463"/>
            <dbReference type="EC" id="3.3.2.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15 16">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15 16">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 16">Lipid metabolism; leukotriene B4 biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.3.2.6" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0IHY3"/>
    <dbReference type="Ensembl" id="ENSEAST00005057502.1">
        <property type="protein sequence ID" value="ENSEASP00005040493.1"/>
        <property type="gene ID" value="ENSEASG00005019525.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156375"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004301">
        <property type="term" value="F:epoxide hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004463">
        <property type="term" value="F:leukotriene-A4 hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019370">
        <property type="term" value="P:leukotriene biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09599">
        <property type="entry name" value="M1_LTA4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000003">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.320:FF:000002">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000004">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2010.30:FF:000001">
        <property type="entry name" value="Leukotriene A(4) hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.320">
        <property type="entry name" value="Peptidase M1, leukotriene A4 hydrolase/aminopeptidase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012777">
        <property type="entry name" value="LTA4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049980">
        <property type="entry name" value="LTA4H_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038502">
        <property type="entry name" value="M1_LTA-4_hydro/amino_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034015">
        <property type="entry name" value="M1_LTA4H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015211">
        <property type="entry name" value="Peptidase_M1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02411">
        <property type="entry name" value="leuko_A4_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45726:SF3">
        <property type="entry name" value="LEUKOTRIENE A-4 HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09127">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01263">
        <property type="entry name" value="Leuk-A4-hydro_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 16" id="KW-0434">Leukotriene biosynthesis</keyword>
    <keyword evidence="7 15 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 16" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6 16" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 15 16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M1 leukotriene A4 hydrolase/aminopeptidase C-terminal" evidence="17">
        <location>
            <begin position="586"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="419"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="13">
        <location>
            <position position="506"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="257"/>
            <end position="259"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="389"/>
            <end position="394"/>
        </location>
        <ligand>
            <name>a peptide</name>
            <dbReference type="ChEBI" id="CHEBI:60466"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="422"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="441"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="686"/>
            <end position="688"/>
        </location>
        <ligand>
            <name>a peptide</name>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004716"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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            <dbReference type="ARBA" id="ARBA00022723"/>
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    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU361141"/>
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            <dbReference type="SMART" id="SM01263"/>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005040493.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
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    <name>A0A9L0IIU6_EQUAS</name>
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        <submittedName>
            <fullName evidence="7">Solute carrier family 26 member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">SLC26A4</name>
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    <organism evidence="7 8">
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        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="gene ID" value="ENSEASG00005017498.2"/>
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    <dbReference type="KEGG" id="eai:106825236"/>
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    <dbReference type="GeneTree" id="ENSGT01150000286920"/>
    <dbReference type="OrthoDB" id="288203at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031526">
        <property type="term" value="C:brush border membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140900">
        <property type="term" value="F:chloride:bicarbonate antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015111">
        <property type="term" value="F:iodide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006885">
        <property type="term" value="P:regulation of pH"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <accession>A0A9L0ILK4</accession>
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    </dbReference>
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</entry>
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    <accession>A0A9L0IM26</accession>
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                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Heart- and skeletal muscle-specific component of the ribosome, which regulates muscle function. Component of the large ribosomal subunit in striated muscle cells: replaces the RPL3 paralog in the ribosome in these cells. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Inhibits myotube growth and muscle function.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044603076.1">
        <property type="nucleotide sequence ID" value="XM_044747141.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IM26"/>
    <dbReference type="Ensembl" id="ENSEAST00005063597.1">
        <property type="protein sequence ID" value="ENSEASP00005041197.1"/>
        <property type="gene ID" value="ENSEASG00005009140.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106836220"/>
    <dbReference type="KEGG" id="eai:106836220"/>
    <dbReference type="CTD" id="6123"/>
    <dbReference type="GeneTree" id="ENSGT00390000017606"/>
    <dbReference type="OMA" id="HVEDGKM"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010832">
        <property type="term" value="P:negative regulation of myotube differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016202">
        <property type="term" value="P:regulation of striated muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000079">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1430.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.960.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.960.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000351">
        <property type="entry name" value="Ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="entry name" value="Ribosomal protein L3, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363:SF7">
        <property type="entry name" value="RIBOSOMAL PROTEIN UL3-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00055008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00077439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005041197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="407" mass="46345" checksum="E484FD9FD6CEAE7D" modified="2023-09-13" version="1">MSHRKFSAPRHGHLGFLPHKRSRRHRGKVKTWPRDDPSQPVHLTAFLGYKAGMTHTLREVHRPGLKISKREEVEAVTIVETPPLVVVGVVGYVATPRGLRSFKTIFAEHLSDECRRRFYKDWHKSKKKAFTKACRRWRDASGKKQLQKDFAAMKKYCKVIRVIVHTQMKLLPFRQKKAHIMEIQLNGGTVAEKVAWAQARLEKQVPVHSVFSQSEVIDVIAVTKGRGVKGVTSRWHTKKLPRKTHKGLRKVACIGAWHPARVGCSIARAGQKGYHHRTELNKKIYRIGRGLHMEDGKVVKNNASTSYDVTDKSITPLGGFPHYGEVNNDFVMLKGCIAGTKKRVITLRKSLLVHHSRGALENIELKFIDTTSKFGHGRFQTAQEKRVFMGPQKKHLEKEKPETSGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0INK5</accession>
    <name>A0A9L0INK5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Transmembrane protease serine 7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Matriptase-3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">TMPRSS7</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Serine protease which preferentially hydrolyzes peptides with Arg at the P1 position.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Forms a heterodimer with SERPINA5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0INK5"/>
    <dbReference type="Ensembl" id="ENSEAST00005058486.1">
        <property type="protein sequence ID" value="ENSEASP00005038658.1"/>
        <property type="gene ID" value="ENSEASG00005011381.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160085"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000033">
        <property type="entry name" value="Transmembrane protease serine 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000065">
        <property type="entry name" value="Transmembrane protease serine 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.10:FF:000003">
        <property type="entry name" value="Transmembrane serine protease 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000040">
        <property type="entry name" value="Transmembrane serine protease 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1220.10:FF:000002">
        <property type="entry name" value="Transmembrane serine protease 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000109">
        <property type="entry name" value="Transmembrane serine protease 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1220.10">
        <property type="entry name" value="EGF-type module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.960">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000082">
        <property type="entry name" value="SEA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036364">
        <property type="entry name" value="SEA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252">
        <property type="entry name" value="ACROSIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24252:SF12">
        <property type="entry name" value="TRANSMEMBRANE SERINE PROTEASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01390">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00261">
        <property type="entry name" value="LDLRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82671">
        <property type="entry name" value="SEA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50024">
        <property type="entry name" value="SEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 17" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0720">Serine protease</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="SEA" evidence="19">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="18">
        <location>
            <begin position="111"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="18">
        <location>
            <begin position="239"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="20">
        <location>
            <begin position="480"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="358"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="365"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="377"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="412"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="433"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="16">
        <location>
            <begin position="453"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00061779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00077326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="717" mass="79773" checksum="2ADE47952C567D1D" modified="2023-09-13" version="1">MFRITNIEFLPEHRQKESREFLSVAQTVQQVVNLVYTTSAFSKFYKQSVVADVSNNKGGLLIHFWIVFVMPHAKGHIFCEDCVAAILKDSIQTSIINRTSVGSLQGLAVDMDSVVLNDKGCSQYFYADHPSFRYPLEISATSGRLMCHFKLVAIVGYLIRFSIESIEIEADNCVTDSLTIYDALLPIRSTILYRICEPTRTLMSFVSTNNLMLVTLKSPQIRSLSGIRAYLEVIPEQKCENTVLVKEIAGFEGKISSPYYPSYYPPKCTCTWKFQAPLPTLGIALKFHNYSITQKSVKGCDHGWWEINEHMYCGSYMDHQTIFRVPRPLVHVQLQCSSRLSDKPLLVEYGSYNISQPCPVASFRCSSGLCVPQAQRCDGVNDCFDESDELFCVSLQPACNTSSSGPRGPLVCDGFRDCEDGQDEQNCTQSIPCNNGTFKCSNDICLRKQNAKCDGTVDCPDGSDEEGCSCSGSSSILHRIIGGTDTQEGGWPWQVSLHFVGVAYCGASVISREWLLSAAHCFHGNRLSDPTPWTAHLGMYVQGNAKFVSPLRRIVVHEYYNSQTFDYDIALLQLSIAWPETLKQLIQPICIPPAGQKVRGGEKCWVTGWGRRHEADNKGSPVLQQAEVELIDQTLCVSTYGIITSRMLCAGVMSGKRDACRGDSGGPLSCRRKSDGKWILTGIVSWGHGCGRPNFPGVYTRVSNFVPWIHKYVPSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IPH8</accession>
    <name>A0A9L0IPH8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123281039</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IPH8"/>
    <dbReference type="Ensembl" id="ENSEAST00005060960.1">
        <property type="protein sequence ID" value="ENSEASP00005038988.1"/>
        <property type="gene ID" value="ENSEASG00005009789.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286907"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033691">
        <property type="term" value="F:sialic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000912">
        <property type="entry name" value="Myeloid cell surface antigen CD33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000829">
        <property type="entry name" value="Sialic acid-binding Ig-like lectin 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051036">
        <property type="entry name" value="SIGLEC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12035:SF132">
        <property type="entry name" value="MYELOID CELL SURFACE ANTIGEN CD33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12035">
        <property type="entry name" value="SIALIC ACID BINDING IMMUNOGLOBULIN-LIKE LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="4" id="KW-0430">Lectin</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040156652" description="Ig-like domain-containing protein" evidence="13">
        <location>
            <begin position="20"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="147"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="234"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="307"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="379" mass="41590" checksum="0A4935B90099450A" modified="2023-09-13" version="1">MLLLLLLLALLWWTEGAKGQRVPLQDYSLRMQGSVTVQEGLCVFVPCDFFYPRDSRTVLTFAHGYWFREGARVYQDASVATNDPDREVNEETQGRFHLLGDPRTYNCSLDIRDARRRDEGQYFFRVEKYSYKSNQLNLRVTALNHTPDILIPETLESGRPRNLTCSVPWACERGTPPIFSWTSAALTSLGPRARLSSVLTLTPRPQDHGTIVTCQVKFPAADVTVETTIQLNVTSPGDKDQCPPSPPTDVSPPCLSTSDSPQNLAVTVFLGNGTAPTTLKNASSLPVQEGQSLRLVCVVRQQPPCQGELGPWDPDSEPLTALEPRGPGAAPGGVESRRRIHLPSSAPLGLPAHHPESLCAEKSETFIRSGAVGCRGSRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IQH2</accession>
    <name>A0A9L0IQH2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transitional endoplasmic reticulum ATPase</fullName>
            <ecNumber evidence="5">3.6.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">15S Mg(2+)-ATPase p97 subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Valosin-containing protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">VCP</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.6" evidence="5"/>
    <dbReference type="RefSeq" id="XP_044635222.1">
        <property type="nucleotide sequence ID" value="XM_044779287.2"/>
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    <dbReference type="AlphaFoldDB" id="A0A9L0IQH2"/>
    <dbReference type="Ensembl" id="ENSEAST00005063813.1">
        <property type="protein sequence ID" value="ENSEASP00005042368.1"/>
        <property type="gene ID" value="ENSEASG00005013806.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106846998"/>
    <dbReference type="KEGG" id="eai:106846998"/>
    <dbReference type="CTD" id="7415"/>
    <dbReference type="GeneTree" id="ENSGT00900000141071"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990730">
        <property type="term" value="C:VCP-NSFL1C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051228">
        <property type="term" value="P:mitotic spindle disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19519">
        <property type="entry name" value="RecA-like_CDC48_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19528">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000004">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.330.10:FF:000001">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000003">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000048">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.150">
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
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    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
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    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF69">
        <property type="entry name" value="TRANSITIONAL ENDOPLASMIC RETICULUM ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
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    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
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    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
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    <dbReference type="SUPFAM" id="SSF50692">
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF54585">
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16" id="KW-0072">Autophagy</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="18" id="KW-0234">DNA repair</keyword>
    <keyword evidence="14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="26">
        <location>
            <begin position="25"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="25">
        <location>
            <begin position="125"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="24">
        <location>
            <begin position="237"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="24">
        <location>
            <begin position="510"/>
            <end position="649"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="765"/>
            <end position="804"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="23">
        <location>
            <begin position="775"/>
            <end position="791"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012674"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019970"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022763"/>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00031475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00031860"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048883"/>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="SMART" id="SM00382"/>
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    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="SMART" id="SM01072"/>
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    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="SMART" id="SM01073"/>
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    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005042368.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IVD5</accession>
    <name>A0A9L0IVD5_EQUAS</name>
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        <submittedName>
            <fullName evidence="8">TLC domain containing 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">TLCD1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
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                <person name="J."/>
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                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="component" value="Chromosome 13"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="TLCD"/>
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    <dbReference type="PANTHER" id="PTHR13439">
        <property type="entry name" value="CT120 PROTEIN"/>
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    <dbReference type="PANTHER" id="PTHR13439:SF5">
        <property type="entry name" value="TLC DOMAIN-CONTAINING PROTEIN 1"/>
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        <property type="entry name" value="TRAM_LAG1_CLN8"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="48"/>
            <end position="67"/>
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    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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    </feature>
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        <location>
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        </source>
    </evidence>
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    </evidence>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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                <consortium name="Ensembl"/>
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    </evidence>
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        <source>
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    </evidence>
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                <person name="X. and Zhong"/>
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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    </dbReference>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Potential calcium-dependent cell-adhesion protein.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IZ53"/>
    <dbReference type="Ensembl" id="ENSEAST00005070799.1">
        <property type="protein sequence ID" value="ENSEASP00005043037.1"/>
        <property type="gene ID" value="ENSEASG00005022753.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158335"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11304">
        <property type="entry name" value="Cadherin_repeat"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000005">
        <property type="entry name" value="Protocadherin 9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000016">
        <property type="entry name" value="Protocadherin 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000030">
        <property type="entry name" value="Protocadherin 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000036">
        <property type="entry name" value="Protocadherin 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000069">
        <property type="entry name" value="Protocadherin-11 X-linked"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.60:FF:000077">
        <property type="entry name" value="Protocadherin-11 X-linked"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.60">
        <property type="entry name" value="Cadherins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002126">
        <property type="entry name" value="Cadherin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015919">
        <property type="entry name" value="Cadherin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020894">
        <property type="entry name" value="Cadherin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013164">
        <property type="entry name" value="Cadherin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013585">
        <property type="entry name" value="Protocadherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050174">
        <property type="entry name" value="Protocadherin/Cadherin-CA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028">
        <property type="entry name" value="CADHERIN-87A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24028:SF254">
        <property type="entry name" value="PROTOCADHERIN-11 X-LINKED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00028">
        <property type="entry name" value="Cadherin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08266">
        <property type="entry name" value="Cadherin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08374">
        <property type="entry name" value="Protocadherin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00205">
        <property type="entry name" value="CADHERIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00112">
        <property type="entry name" value="CA"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49313">
        <property type="entry name" value="Cadherin-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00232">
        <property type="entry name" value="CADHERIN_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50268">
        <property type="entry name" value="CADHERIN_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 12" id="KW-0106">Calcium</keyword>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 15" id="KW-0732">Signal</keyword>
    <keyword evidence="3 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040323249" evidence="15">
        <location>
            <begin position="24"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="813"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="53"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="140"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="250"/>
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        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
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            <begin position="362"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="467"/>
            <end position="570"/>
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    <feature type="domain" description="Cadherin" evidence="16">
        <location>
            <begin position="571"/>
            <end position="673"/>
        </location>
    </feature>
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        <location>
            <begin position="685"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1016"/>
            <end position="1111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="ARBA" id="ARBA00022889"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00043"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="1111" mass="122490" checksum="1CDEC945BD3D4203" modified="2023-09-13" version="1">MDLLSGTYIFAVLLACVVFQSGAQEKNYTVREEMPENVLIGDLLKDLNLSLIPDKSLTSPMQFKLVYKTGDVPLIRIEEGTGEIFTTGARIDREKLCAGILVEARCFYEVEVAVLPDEIFRLVKIRFLIEDINDNAPLFPATVINISIPENSAINSRYALPAAIDPDIGINGVQNYQLIKGQSIFGLDVIETPEGDKMPQLIVQKELDREEKDTYVMKVKVEDGGFPQRSSTAILQVSVADTNDNRPVFKENEIEVSIPENAPVGTSVTQLHATDADIGENAKIQFYFSNLVSNIAKRLFHLNTTTGLITIKEPLDREESPNHKLLVLASDGGLMPARAMVLVNVTDINDNVPSIDIRYIINPINGTVVLSENAPLNTKIALITVTDKDADHNGRVTCFTDHEVPFRLRPVFSNQFLLETAAYLDYESTREYAIKLLAADAGKPPLNQSSMLLVKVKDENDNAPVFTQPFISLSVPENNSPGTQLTKISATDADSGRNAEISYLLGIDAPSEFNLDRRTGILTAVKKLDREKQEKYSFTVLAKDNGIPPLTTNATVLVTVLDQNDNSPIFTHNEYNFYVPENLPRHGTVGLITVTDPDYGENSAVTLSILDVNDDFTIDPQTGVIQPNISFDREKQESYTFYVKAEDGGRVSRSSTAKVTINVVDVNDNKPVFVVPSSNYSYELVLPSTNPGTVVFTVVAVDNDTGMNAELRYSIVGGNTKGLFIIDQTTGNITLKEKCVVSDLGLHRLVVKAKDLGQPDSLFSAVIVNLFVNESVTNATLIHELVRKNIETPVTQNIETADSSSPTSDYVKIMVAIVAGTITVILVIFITAVVRCRQSPHLKAAQKNKQNSEWVTPNPENRQMIMMKKKKKKKKKHAPKNLLLNFVTIEETKADDVDNDGNSVTLDLPIELEEQTMGKYNWGTTPTTFKPDSPDLARHYKSASPQPAFQIQPETPLNSKHHIIQELPLDNTFVACDSISKCSSSSSDPYSVSECSYPVTTFKTPVSVHTRPPVKEVVRSRTPMKEATTVEIWTHPQPQSQRRVTFHLPEGSQESSSDGGLGDHDGGSLPSTSHALPLGYPQEEYFDHAAPNNRTEGDGNSDPESRRLALS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J0E8</accession>
    <name>A0A9L0J0E8_EQUAS</name>
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        <recommendedName>
            <fullName evidence="3">GTP-binding protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">GTPBP8</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Sun"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
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                <person name="Zhao"/>
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                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Ji"/>
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                <person name="Jin"/>
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                <person name="G."/>
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                <person name="Xu"/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
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                <person name="X. and Zhong"/>
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            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family.</text>
    </comment>
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        <property type="project" value="TreeGrafter"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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            <authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="59" mass="6881" checksum="EDDBDA969D0D1FD4" modified="2023-09-13" version="1">MIVLGWMLFVGLACYMGTFPEFLPPTLKWKERWPVQESKARLRSRAVDEDLQLNNVEGI</sequence>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="18"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0J3N0"/>
    <dbReference type="Ensembl" id="ENSEAST00005071277.1">
        <property type="protein sequence ID" value="ENSEASP00005047759.1"/>
        <property type="gene ID" value="ENSEASG00005019192.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011669"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015616">
        <property type="term" value="F:DNA translocase activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000042">
        <property type="term" value="P:negative regulation of double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902231">
        <property type="term" value="P:positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072429">
        <property type="term" value="P:response to intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd22095">
        <property type="entry name" value="F-box_FBXO18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18786">
        <property type="entry name" value="SF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000011">
        <property type="entry name" value="F-box DNA helicase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000833">
        <property type="entry name" value="F-box DNA helicase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070:SF30">
        <property type="entry name" value="F-BOX DNA HELICASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="24">
        <location>
            <begin position="227"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="50"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="119"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="189"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
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            <dbReference type="ARBA" id="ARBA00022840"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00071173"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00079567"/>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="24">
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            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
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    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
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        </source>
    </evidence>
    <sequence length="1062" mass="120320" checksum="B2EA6CE9DAC0FDDF" modified="2023-09-13" version="1">MITNECPVSSASCYKTVRRFKRKHLLAIDCQHLARSHLAVAQPFGQRWTNRDPNHGLYPRPRTKRGTRGQGCQRRITEFFLAGRQRCTNDMAKSNSVGRDSSKDSEGEMIFAAESNCALPQEGDGEARLGSSGSALPARKRSRSFEDERNQATGTSQWDGVSKKTPRHRLSLSCTKPTEARQEAEDSLSWLSAESGESSQEVEDIGPDPIPDSYYGLLGTLPCQEPQSHICSLPSEVLRHIFAFLPVEDLYWNLSLVCHLWREIISDPLFIPWKKLYHRYLMNEEQAVSKVDGILLNYGIEKESELCVLNLIRYTATTKCSPSVDPGRALWSLRDHLLLPEAEACVRQHLPDLYVAAAGVNVWALVAAIVLLSSSVNDIQQLLFCLRRPSSTVTMPDITETLYCIAVLLYAMREKGINISNRIHYNVFYCLYLQENSSTQATEVKEEPSVWPGKKTTIRLTHEQQLILSHKMEPLQVVKIMAFAGTGKTSTLVKYAEKWSESRFLYVTFNKSIAKQAELVFPSNVTCKTFHSMAYGHVGRKYQLKKKLNLFKLTPFMVNSVLAEGKGGFIRAKLVCKTLENFFASADEELTIDHVPIWCKNSQGQRVMVEQSEKLNGVLEASRLWDNMRKLGECKEEAYQMTHDGYLKLWQLSKPLLASFDAIFVDEAQDCTPAIMNIVLSQPCGKIFVGDPHQQIYTFRGAVNALFTVPHTHVFYLTQSFRFGVEIAYVGATILDVCKRVRKKTLVGGNHQSGIRGDVQGQVALLSRTNANVFDEAVRVTEGEVPARIHLIGGIKSFGLDRIIDIWILLQPEEERRKQNLVIKDKFIRRWVHKEGFSGFKRYVTAAEDKELEAKIAVVEKYNIRIPELVERIEKCHIEDRDFAEYILGTVHKAKGLEFDTVHVLDDFVKVPCARHNLAQLPHFRVESFSEDEWNLLYVAVTRAKKRLIMTKSLENILTLAGEYFLQAELTSNVLKTGVVRCCVGQCNNAIPVDTVLTMRKLPITYSNRKENKGGYLCHSCVEQRIGPLAFLTASPEQVRSMERTVEDLVLPRHEALLFLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J4W2</accession>
    <name>A0A9L0J4W2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">(S)-2-hydroxy-acid oxidase</fullName>
            <ecNumber evidence="2">1.1.3.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">HAO2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2-hydroxyoctanoate + O2 = 2-oxooctanoate + H2O2</text>
            <dbReference type="Rhea" id="RHEA:67940"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:133514"/>
            <dbReference type="ChEBI" id="CHEBI:176689"/>
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        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:67941"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a (2S)-2-hydroxycarboxylate + O2 = a 2-oxocarboxylate + H2O2</text>
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            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:35179"/>
            <dbReference type="ChEBI" id="CHEBI:58123"/>
            <dbReference type="EC" id="1.1.3.15"/>
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        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:16790"/>
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    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
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    <comment type="similarity">
        <text evidence="5">Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.</text>
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    <dbReference type="EC" id="1.1.3.15" evidence="2"/>
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    <dbReference type="AlphaFoldDB" id="A0A9L0J4W2"/>
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    <dbReference type="GeneID" id="106832921"/>
    <dbReference type="CTD" id="51179"/>
    <dbReference type="GeneTree" id="ENSGT00390000018717"/>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
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            <begin position="20"/>
            <end position="406"/>
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            <begin position="52"/>
            <end position="406"/>
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        <location>
            <position position="78"/>
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        <ligand>
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        <ligand>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
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                <person name="Jin"/>
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                <person name="Yue"/>
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                <person name="Liu"/>
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                <person name="Xu"/>
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                <person name="Liu"/>
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                <consortium name="Ensembl"/>
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                <consortium name="Ensembl"/>
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            <topology evidence="1 6">Multi-pass membrane protein</topology>
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        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR14297:SF4">
        <property type="entry name" value="XK-RELATED PROTEIN 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="XK-related"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
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            <end position="54"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <end position="370"/>
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    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="382"/>
            <end position="405"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008789"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="449" mass="51877" checksum="13BBE2B45FCF8DD3" modified="2023-09-13" version="1">MDRVYEIPEEPSLDPISSLEENVIRGANPRFTFPFGILFSTFLYCGEAASALYVVRIYRKNSETYWMAYTFSFFMFSSIMVQLTLIFVHRDLAKDKPLSLFMHLILLGPVIRCLEAMIKYLTLWKKEGQEEPYVSLTRKKMLINGEEVLIEWEVGHSIRTLAMHRNAYKRMSQIQAFLGSVPQLTYQLYVTLISAEVPLGRAVLMVFSLISVTYGATLCNMLAIQIKYDEYKIRLGPLEVLCITIWRTLEITSRLMILVLFSATLKLKALPFLLLNFLIILFEPWVKFWRSGAQMPNNIEKNFSRVGTLVVLISITVLYAGINFSCWSALQLKLADRDLVDKGQNWGHMGLHYSVRLVENVIMVLVFKFFGVKVLLNYCHSLIALQLIIAYLISIGFMLLFFQYLHPLRSLFTHNVVDYLHCVCCRRHPRGRVEDLEPSVDAETRQSIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J801</accession>
    <name>A0A9L0J801_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 774</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZNF774</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014698097.1">
        <property type="nucleotide sequence ID" value="XM_014842611.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J801"/>
    <dbReference type="Ensembl" id="ENSEAST00005053799.1">
        <property type="protein sequence ID" value="ENSEASP00005049426.1"/>
        <property type="gene ID" value="ENSEASG00005032976.1"/>
    </dbReference>
    <dbReference type="GeneID" id="123275562"/>
    <dbReference type="KEGG" id="eai:123275562"/>
    <dbReference type="CTD" id="342132"/>
    <dbReference type="GeneTree" id="ENSGT00940000163116"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000214">
        <property type="entry name" value="replication initiator 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000056">
        <property type="entry name" value="Zinc finger and SCAN domain-containing 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000258">
        <property type="entry name" value="zinc finger and SCAN domain-containing protein 29 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000418">
        <property type="entry name" value="zinc finger protein 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000360">
        <property type="entry name" value="zinc finger protein 572"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000202">
        <property type="entry name" value="Zinc finger protein 574"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000933">
        <property type="entry name" value="zinc finger protein 771"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001385">
        <property type="entry name" value="zinc finger protein 774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001450">
        <property type="entry name" value="zinc finger protein 774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001714">
        <property type="entry name" value="Zinc finger protein 774"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000188">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF152">
        <property type="entry name" value="SI:DKEY-210J14.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13465">
        <property type="entry name" value="zf-H2C2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="152"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="180"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="208"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="236"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="264"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="292"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="320"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="348"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="376"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="404"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="432"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="460"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="89"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005049426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="484" mass="55127" checksum="EF8D71EB5EBFF8CD" modified="2023-09-13" version="1">MMWLGTSGKSGLPGPCLQNPLQGHHPAHVEEWALNGISRPSVISQLEQKEEPWVLPLPNFEVRKILRESHTDFENQVVKLNRDISETAEQCGTSSARANKDISQTPSWGGNWERGLELEGQHGTLPREGHQGPFSQEKDLNKLLAGYVGKKPVCEECGKSFNQSSYLVRHLRTHTGERPYKCIECGKGFKQSSDLVTHRRTHTGEKPYQCSGCERKFSDSSTLVKHQRTHTGERPYECPECGKTFGRKPHLIMHQRTHTGEKPYTCLECHKSFSRSSNFITHQRTHTGVKPYRCHNCGENFSQSSDLTKHQRTHTGERPFQCPECGKGFRDSSHFVAHMSTHSGERPFSCPHCHKSFSQSSHLATHQRTHTGERPFKCDDCGKGFTDSSALVKHQRIHTGERPYKCGECGKSFNQSSHFITHQRIHLGDRPYPCPECGKTFNQRSHFLTHQRTHTGEKPFHCSKCDKSFRQKAHLLCHQNTHLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J9A3</accession>
    <name>A0A9L0J9A3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Negative regulator of P-body association</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">NBDY</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="component" value="Chromosome 6"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="68" mass="7280" checksum="BC44540627963296" modified="2023-09-13" version="1">MGDQPCVSGRSMLPTGDTREIKPPKKRCLLAPRWDYPEGTPNGGITPLPSAPPPASPSLKLHPLPPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J9L9</accession>
    <name>A0A9L0J9L9_EQUAS</name>
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        <recommendedName>
            <fullName evidence="14">Receptor activity-modifying protein 2</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Accessory protein that interacts with and modulates the function of G-protein coupled receptors including calcitonin gene-related peptide type 1 receptor (CALCRL) and calcitonin receptor (CALCR). Required for the transport of CALCRL to the plasma membrane. Together with CALCRL, form a receptor complex for adrenomedullin/ADM. Together with CALCR, act as a receptor complex for calcitonin/CT/CALC. Together with CALCR, also act as a receptor complex for amylin/IAPP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Heterodimer of CALCRL and RAMP2; the interaction forms the receptor complex for adrenomedullin/ADM. Heterodimer of CALCR and RAMP2; interaction forms the AMYR2 receptor complex for calcitonin/CALC and amylin/IAPP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RAMP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J9L9"/>
    <dbReference type="Ensembl" id="ENSEAST00005067000.1">
        <property type="protein sequence ID" value="ENSEASP00005045930.1"/>
        <property type="gene ID" value="ENSEASG00005010035.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
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        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:coreceptor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032870">
        <property type="term" value="P:cellular response to hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031623">
        <property type="term" value="P:receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008277">
        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.510:FF:000003">
        <property type="entry name" value="Receptor activity-modifying protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.510">
        <property type="entry name" value="Receptor activity modifying family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006985">
        <property type="entry name" value="RAMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038126">
        <property type="entry name" value="RAMP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14076">
        <property type="entry name" value="RECEPTOR ACTIVITY MODIFYING PROTEIN RAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14076:SF9">
        <property type="entry name" value="RECEPTOR ACTIVITY-MODIFYING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04901">
        <property type="entry name" value="RAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="92"/>
            <end position="113"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="64"/>
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        </location>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005045930.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="285" mass="31111" checksum="8D573B08E366BDF4" modified="2023-09-13" version="1">GSCPAPQGKLEEREGQRQPGGGSGGRQDRVGQRLPLQPASPGRRPRPPRGRSRPSPCEEAARLPAAPAGDIGAPARTRLRAAGPLRLLAARWPRSGRSAPPAARGSLRPAPGDRQCSASSSCWAAPLPRSLPSHLPLPFLPTAVLKPQESLAQLLLTPGSLESEGKTVENYETNVQLCWQEYKVYMDSIQKDWCDWAMISRPYSILQYCLEQTAEGFGLGFPNPWAERIIFETHQIHFANCSLVQPTFSDPPEDVLLAMIIAPICLIPFLVTLVVWRSKDSEAQA</sequence>
</entry>
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    <accession>A0A9L0JC31</accession>
    <name>A0A9L0JC31_EQUAS</name>
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        <recommendedName>
            <fullName evidence="3">Immunoglobulin V-set domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="J."/>
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                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
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                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JC31"/>
    <dbReference type="Ensembl" id="ENSEAST00005070626.1">
        <property type="protein sequence ID" value="ENSEASP00005050744.1"/>
        <property type="gene ID" value="ENSEASG00005029883.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01090000263325"/>
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        <property type="component" value="Chromosome 10"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051117">
        <property type="entry name" value="TRG_var/const_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19256:SF44">
        <property type="entry name" value="T CELL RECEPTOR GAMMA VARIABLE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19256">
        <property type="entry name" value="T-CELL RECEPTOR GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="1" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Immunoglobulin V-set" evidence="3">
        <location>
            <begin position="26"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="120" mass="13611" checksum="18551D821DE8A34B" modified="2023-09-13" version="1">MLSLFQALPLAVLEAYKLFQPQLSSTKRLSKTATWNVWYLVTISTMSVYWYQESPGQVLQPLLHVSSDNTVRMESGVPMGKFKVDEIPEPSMSTLTTHNVGTEDLATYYCAFWEVHSGRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JDB6</accession>
    <name>A0A9L0JDB6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Kinesin-like protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106846362</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Microtubule-dependent motor protein required for mitochondrion morphology and transport of mitochondria in neuronal cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JDB6"/>
    <dbReference type="Ensembl" id="ENSEAST00005063875.1">
        <property type="protein sequence ID" value="ENSEASP00005051179.1"/>
        <property type="gene ID" value="ENSEASG00005036075.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163104"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22709">
        <property type="entry name" value="FHA_KIF28P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000063">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000034">
        <property type="entry name" value="kinesin-like protein KIF28P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022140">
        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12423">
        <property type="entry name" value="KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8 11" id="KW-0505">Motor protein</keyword>
    <keyword evidence="3 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Kinesin motor" evidence="12">
        <location>
            <begin position="1"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="106"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00054688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00079247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005051179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1008" mass="113181" checksum="4FCB081919895303" modified="2023-09-13" version="1">KDISWGGQFPAPTVREKNSGSKCVISMHSGTTTTIQDPKNPEHVKTFTFDLAYWSHSGFQMDKDGVFISADPSGKFAGQRDVFRDLGGGILDSAWQGYNATLLAYGQTGSGKSYSMIGFGANKGLIPSVCEELFQAIENRERNQEYQVTFSMLEIYNEQVRDLLSRTKKPGGLKVREDQQLGFYVDGLKSVPCENYAQIERLMEQGTRLRTTASTNMNASSSRSHMVITIQFKQVFLDRELTKQSSINLVDLAGSERQKSSGSEGDRLREGSRVNLSLTNLGNVISALADAAMGKRVLHVPYRDSVLTKLLQSALGGNSRTTLIAAISPADICYEETLSTLRYAERAKKIRNKAVVNTSTLMRESRTENNKLLLGCGNSSMAGQPAYLLAEHGLGPHADPMSWAHQLEQARKEWQQQYVAIARERQMTETLPHLLNVNEDPQLTGVLKYFIQPGSCDAGQATSNAITLHGLGISEKHASFTNLDGKVTVTPHSKCKVVVNGVPITTATKLQHLDRIILGSNSAYLYIGFPSERGSEDLSRFDYDFFQLERAAAEGVSVDKLGAVNSGDGKADPSVLAVFQDYIKLMPLVAEANQMSEELGKGLKMELKVKNLALSDSRGYDLQKEVRVPLWVWIWSKAKFINRKFLMEELYQRFLDGEDSHVAQEDDPFWDPLEVIHLGSAHIWLQSLAYCMKLEEQVEFLNCDGLEEAVLHIHISPCSPTGQACGEEDVVIDPLELLGKRMDFQIRIVRCLGAKWLKEDAERGVQIGYRIYDLPNTLYTKPVWKTVNPQIEETVQFTALNASQEFLNYLQTNALVVDLWGLQEGCAELSCCEPDLMITGEGHIMVDTKKISTSTSNQISELYLKLLKLEQETELLRNINRALREENVLLKASLEKTGSSQQGERLLESWWFTARDINQVCSQQASYDREFAKALKVFYQSMNMARGQFLRLRLYKPPEDDQMLRPFVHQQSQMLKDFGDLLESSLWKLKNDVALIVKKKRECLLRVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JDN9</accession>
    <name>A0A9L0JDN9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein 218</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TMEM218</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in ciliary biogenesis or function.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TMEM218 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JDN9"/>
    <dbReference type="Ensembl" id="ENSEAST00005063764.1">
        <property type="protein sequence ID" value="ENSEASP00005051426.1"/>
        <property type="gene ID" value="ENSEASG00005023774.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016247"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="Tmem218"/>
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    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="6"/>
            <end position="31"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="43"/>
            <end position="64"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="84"/>
            <end position="107"/>
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    </feature>
    <feature type="domain" description="Transmembrane protein 218 N-terminal" evidence="11">
        <location>
            <begin position="4"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00010775"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00015054"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF25810"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
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            <dbReference type="Ensembl" id="ENSEASP00005051426.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="118" mass="12786" checksum="9D32CE8949A1B6DB" modified="2023-09-13" version="1">MGEMAGTVLGVGAGVFILALLWVSVLLLCVLLSRASGIARFSVIFIFLGALIITAVLLLFPRAAEVPAPEVEMKIVDAFFIGRYVLLAFLTAVFLGGLFLVLIHYILEPIYAKPLRSY</sequence>
</entry>
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    <name>A0A9L0JGJ5_EQUAS</name>
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        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 24</fullName>
        </alternativeName>
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        <name evidence="9" type="primary">MED24</name>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 24 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_070483208.1"/>
    </dbReference>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_070483210.1"/>
    </dbReference>
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        <property type="gene ID" value="ENSEASG00005002779.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 13"/>
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    </dbReference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12898">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 24"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11277">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007864"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00019693"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031960"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
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        </source>
    </evidence>
    <sequence length="989" mass="110123" checksum="B8E41031A30149EA" modified="2023-09-13" version="1">MKVVNLKQAILQAWKERWSDYQWAINMKKFFPKGATWDILNLAEALLEQAMIGPSPNPLILSYLKYAISSQMVSYSSVLTAISKFDDFSRELCVQALLDIMDMFCDRLSCHGKAEECIGLCRALLSALHWLLRCTAASAERLREGLEAGSPATGEKQLAMCLQRLEKTLSSTKNRALLHIAKLEEASSWTAIEHSLLKLGEILANLSNPQLRSQAEQCGTLIRSIPTMLSVHSEQLHKTGFPTVHAVVLLEGTMNLTGETQPLVEQLMMVKRMQHIPTPLFVLEIWKACFVGLIESPEGTEELKWTAFTFLKIPQVLVKLKKYSHGDKDFTEDVNCAFEFLLKLTPLLDKADQRYNCDCTDFLLQECGKQGLLSEASVNNLVAKRAADRKHVPQLKSSENANIQPNPGLILRAEPTVTNILKTMDADHSKSPEGLLGVLGHMLSGKSLDLLLAAAAATGKLKSFARKFINLNEFTTHGSGESTKAASVRALLFDISFLMLCHVAQTYGSEVILSESSAGAEVPFFETWMQTCMPEEGKILNPDHPCFRPDSTKVESLVALLNNSSEMKLVQMKWHEACLSISAAILEILNAWENGVLAFESIQKITDNIKGKVCSLAVCAVAWLVAHVRMLGLDEREKSLQMIRQLAGPLYSENTLQFYNERVVIMSSILEHMCADVLQQTATQIKFPSTGVDTMPYWNLLPPKRPIKEVLTDIFAKVLEKGWVDSRSIHIFDTLLHMGGVYWFCNNLIKELLKETRKEHTLRAVELLYSIFCLDMQQVTLVLLGHILPGLLTDSSKWHSLMDPPGTALAKLAVWCALSSYSSHKGQASSRQKKRHREDIEDYISLFPLDDMQPSKLMRLLSSNEEDANILSSPTDRSMSSSLSASQLHTVNMRDPLNRVLANLFLLISSILGSRTAGPHTQFVQWFMEECVDCLEQGSRGSILQFMPFTTVSELVKVSAMSSPKVVLAITDLSLPLGRQVAAKAIAAL</sequence>
</entry>
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            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
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                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="J."/>
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                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22656">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22656:SF1">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
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        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="4">
        <location>
            <begin position="206"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="2130" mass="238792" checksum="01A6D36761384804" modified="2023-09-13" version="1">METKVHLFYQAEENTNLSEDDCNSFATHLPSIYIDSKKYIKFSKRTEKRISPEIRGLSLEHRKMYETSVCLCGEEKSSDFSREKKVRRKKSLQAQLHTERTEIASSSAKLSKEKMTRKENSLCKLPNQYSIHKTLLPLCTSSSIIRKKERLSNLYDTLYDEVPHGHLHSQQLSALQKACKIFSKIRSGKIYVNDLPMIHCILKISISDSEMRKALKTVDIDVNGMLDFSDFLKAVNDVCYLVSQDPAFKGALKIFCGIKGGRVAVDEVAAVLDSMDIPVTPETLQEVIKHANIDNNHMVNIGDIIFTLGELQQQYEDVSIMEGSALDETTSKRKLSNVSGCYLQYRKKNIFSSRQPEPSLSQKIIRKGPQDCKIMEENDDFEFKRSKNTLQMRKFLDDVDSSDVGFQEPYAKDGVNFDRSSEKMEIHDSKSKPQNLKSITSLKKSLDKSDIFSTPKLQKTAVRRHSSLLKQISSKEKIVINALENVSEAISKLQENYLAAEELQSILPSIGITLSDKEFKKIVTDTTRNENGMVKLDDFMSALSKEQSLPGYDVLTDVVKAIDKIKDKNIDCEDLNACLQNFGVYLSKPEFEKIIELTEADENKNVNFKEFIDTMMSNMELFSEKLLLPRTVENLHNLSKEKMTVSDLWKTLSGLNSNLKKDEFLAVLELGTVGEDNVVNVKDCIKALRDTQKFSNFIALKETIDTLDCVTESNQSAKDKYSDVLENTDRTYFTDDSLQEVLNDFFLEDLRKKTLSSNLKLPKADAIKEAAHILSHVDNGKIGIPNLERALKNLNVNLTEEDFNEALKSCDMSDSKEVDLKDFLKGIRESPHFKESVATQLLLATTQILQNDLIDVSDLKALLMKDDLHAANALLDAVSRHLPEHENGKIAIQEFMIKFSNTLTNPEAIGIEDAWNIVNGVCDGKVEVKDLLSTLESLGKPLNENQLKVLMNSPTETSEVSLKQILETLNTSKPAPEFEDTRAALNTVNVMSCNRIQVNDLKDAFDDLNVCLKPEEHQMLEKTLDVDEKGNVSLKSALLALKSNKRFQDFREVSELAKALDKVTHEKIDVDDVKAVLQGLGIYFPEEELQEVLTSISVDDEGKVDLKDCLTRLMQTPYFTKGSKIDNLLKVLVSIRKNVANPDDLGSMMKSIGVPLPQDVIERALKNVALTEDGTVNLEEFMGNLVNSEFSSLLERNRIDDSNLDTFLGNMRMEMSVDKTVNKNEIKDRRSSMGRKIDFSNVDAVLKNMDIKLTEDEQQELLEHLSATTDEDMDMETLIGIVKMLKEKIEICNLDNFLDDMGIELTYKEYNELVNHLPTSADGKINQLKLVDTMMTLKGGKVNTQKLSNVLEKMGVDLTNKEFQNLREHLPADADRQVELNKLVNEAKAVTGLQVDVHNLDTILGNMAIKLTAEDVNDLTLNLPVDGGEVVFNNMKKVPGNMGIELKDEKHWELVNNLPTDGGNISVSNLDSILGEMRIKLTEKEQEKLTENLLLAGGIVDVTKLDTVLQNVGMKLTGTESKDLIKNLAVDDNGKVKMKKLMDALKAFTGKKVDASDLVKVLGNMGIELTEGEFMKLEQTIPIDAAGQVYQNRLLDGVKSLKGGMVKVDDLDTVLENLGFKLTEQEHEDLTKNLPLNTNRKVHLNKLVNTVETITGEEVDVSDVEDVLGKMGITFTNKEFMKLLENLPLDANEKVYKKRLLGALKSLNGGVIDVNKLDAVLGNMGLNLTEEEIKDLKRNLPTDADEKVEIRKLWDGLKAFTGQKIDVQYLPDFLSNMGIELTDKEQMELLKMLPVDAAGKICENRLLDDVKSFKGGKVERCKINTVLEKMGSKLTEEEIKSLTDNLPVNANGKVDLDKLMEGVKALTGGEIDVSDVENVLGNMGIELTEKEWLKLLKNVPVDAVGKTYQNRLMSSIKSLRGGKVDISNLKPLLEKMDIKLTKKELVQLRENLPTDANGKVDLSKMMDGVKTVTGGNVDINDVNTVLENMGIELKDKEGLELVKNLPFDDDDKIFQNRLLEVVKSLKGGNVNVNNLSTILDNMGIKLADEELRDLTQNLPVDVDKKISLETLMNKVKAFTGEKIDSSDLKNVLGNLGIELTDKEKEKLLETLPIDAAGKVYEKRLLKGLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JHH3</accession>
    <name>A0A9L0JHH3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Acid sphingomyelinase-like phosphodiesterase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">SMPDL3B</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Lipid-modulating phosphodiesterase. Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. Acts as a negative regulator of Toll-like receptor signaling. Has in vitro phosphodiesterase activity, but the physiological substrate is unknown. Lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP (in vitro).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="24">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="24">Binds 2 Zn(2+) per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Interacts with TLR4, TLR7, TLR8 and TLR9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 23">Belongs to the acid sphingomyelinase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044626106.1">
        <property type="nucleotide sequence ID" value="XM_044770171.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JHH3"/>
    <dbReference type="Ensembl" id="ENSEAST00005073692.1">
        <property type="protein sequence ID" value="ENSEASP00005051798.1"/>
        <property type="gene ID" value="ENSEASG00005009506.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106839138"/>
    <dbReference type="KEGG" id="eai:106839138"/>
    <dbReference type="CTD" id="27293"/>
    <dbReference type="GeneTree" id="ENSGT00950000183182"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016798">
        <property type="term" value="F:hydrolase activity, acting on glycosyl bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004767">
        <property type="term" value="F:sphingomyelin phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006685">
        <property type="term" value="P:sphingomyelin catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00842">
        <property type="entry name" value="MPP_ASMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000057">
        <property type="entry name" value="Acid sphingomyelinase-like phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017064">
        <property type="entry name" value="ASM-like_Pdiesterase_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045473">
        <property type="entry name" value="ASM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041805">
        <property type="entry name" value="ASMase/PPN1_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340:SF25">
        <property type="entry name" value="ACID SPHINGOMYELINASE-LIKE PHOSPHODIESTERASE 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10340">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19272">
        <property type="entry name" value="ASMase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036767">
        <property type="entry name" value="ASM-like_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="17" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="20" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="10 23" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0391">Immunity</keyword>
    <keyword evidence="18" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="6" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="13" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="19" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 23 24" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 23" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 23" id="KW-0732">Signal</keyword>
    <keyword evidence="11 23 24" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="23">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040557356" description="Acid sphingomyelinase-like phosphodiesterase" evidence="23">
        <location>
            <begin position="19"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="25">
        <location>
            <begin position="22"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingomyelin phosphodiesterase C-terminal" evidence="26">
        <location>
            <begin position="293"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="279"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00058717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00063194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRNR" id="PIRNR036767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR036767-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF19272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005051798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="456" mass="50713" checksum="6BA28EFB7DF4A202" modified="2023-09-13" version="1">MRLLKLLIFLAHWGVARAGLGKFWHISDLHLDPDYKVSEDPLQVCPSAGSQPVPSAGPWGDHLCDSPWVLINSSIYAMKEIEPEPDFILWTGDDTPHVPNEKLGEAAVLEIVERLTKLIREVFPDTKVYAALGNHDFHPKSQLPPGSNNIYNQVAEMWRPWLSNESIALFREGAFYSEKLPGPSGAGRIVVLNTNLYYSNNEQTAGMADPGQQFQWLEDVLTNASRAGDMVYIIGHVPPGFFEKTRNKAWFREGFNEEYLKVVQKHHRVIAGQFFGHHHTDSFRMFYDDAGAPISVMFLTPGVTPWKTTLPGVVNGANNPGIRVFEYDQATLSLQDMVTYFVNLSQANAQGAPSWELEYRVTEAYGVPNASARSMHAALDRIANDQGALQRYYAYNSVSYDLQACGEACRTEHVCALREVAFDAYSACLRAPGAAPAPRLALLFTALLGLRTLLAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JIZ2</accession>
    <name>A0A9L0JIZ2_EQUAS</name>
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        <recommendedName>
            <fullName evidence="9">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="ENSEASG00005037401.1"/>
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        <property type="component" value="Chromosome 5"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_4"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
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    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="15"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="48"/>
            <end position="66"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="136"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="9">
        <location>
            <begin position="31"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
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    </evidence>
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        <source>
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    <sequence length="182" mass="20544" checksum="618D795FEC4DB5CE" modified="2023-09-13" version="1">MFLLMGFTGDVDVQVFLFLLFLAICLFTLIGNLELVVFFIGDSWLHDPMYYFLSLSSFLDACYSSVVIPKMLINFLLENQTISFLVYAAQMFSYDIFGATECFILAAMVYDCYVAIYNPLLYSVNMSLRVHVPLSIAFYVGGIFKASVHTGAAFSLSFCSSNEIRTFFYDIPPVFAISFSDT</sequence>
</entry>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
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                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
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                <person name="Wang"/>
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                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Liu"/>
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                <person name="Pan"/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
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                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
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                <person name="Wu"/>
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                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="4" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
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        <property type="project" value="InterPro"/>
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    <dbReference type="InterPro" id="IPR042457">
        <property type="entry name" value="TSTD1_mml"/>
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    <dbReference type="PANTHER" id="PTHR45544">
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        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="Pfam" id="PF00581">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
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    </dbReference>
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    <proteinExistence type="predicted"/>
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        <location>
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        </location>
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            <begin position="1"/>
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        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="active site" description="Cysteine persulfide intermediate" evidence="1">
        <location>
            <position position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat G protein beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044627052.1">
        <property type="nucleotide sequence ID" value="XM_044771117.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044627053.1">
        <property type="nucleotide sequence ID" value="XM_044771118.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044627056.1">
        <property type="nucleotide sequence ID" value="XM_044771121.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070365482.1">
        <property type="nucleotide sequence ID" value="XM_070509381.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070365483.1">
        <property type="nucleotide sequence ID" value="XM_070509382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JL74"/>
    <dbReference type="SMR" id="A0A9L0JL74"/>
    <dbReference type="Ensembl" id="ENSEAST00005075370.1">
        <property type="protein sequence ID" value="ENSEASP00005050722.1"/>
        <property type="gene ID" value="ENSEASG00005016867.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106829312"/>
    <dbReference type="KEGG" id="eai:106829312"/>
    <dbReference type="CTD" id="59345"/>
    <dbReference type="GeneTree" id="ENSGT01000000214413"/>
    <dbReference type="OMA" id="GDTNCAL"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000007">
        <property type="entry name" value="Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016346">
        <property type="entry name" value="G-protein_beta_1-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19850">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN BETA G PROTEIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25391">
        <property type="entry name" value="WD40_Gbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002394">
        <property type="entry name" value="GN-bd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="51"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="139"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="180"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="222"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="273"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="308"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="340" mass="37611" checksum="756DDBCBC8E2F562" modified="2023-09-13" version="1">MSELEQLRQEAEQLRNQIQDARKACNDATLIQITSNMDSVGRIQMRTRRTLRGHLAKIYAMHWGYDSRLLVSASQDGKLIIWDSYTTNKMHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELPGHTGYLSCCRFLDDSQIVTSSGDTTCALWDIETAQQTTMFTGHSGDVMSLSLSPDMRTFVSGACDASSKLWDIRDGMCRQSFTGHVSDINAVSFFPNGYAFATGSDDATCRLFDLRADQELLLYSHDNIICGITSVAFSKSGRLLLAGYDDFNCNVWDTLKGDRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLRIWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JLI5</accession>
    <name>A0A9L0JLI5_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 599</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZNF599</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JLI5"/>
    <dbReference type="Ensembl" id="ENSEAST00005044776.1">
        <property type="protein sequence ID" value="ENSEASP00005054079.1"/>
        <property type="gene ID" value="ENSEASG00005027248.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106829206"/>
    <dbReference type="KEGG" id="eai:106829206"/>
    <dbReference type="CTD" id="148103"/>
    <dbReference type="GeneTree" id="ENSGT00940000159953"/>
    <dbReference type="OrthoDB" id="9548399at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004682">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004935">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:005166">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000040">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000348">
        <property type="entry name" value="zinc finger protein 260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000352">
        <property type="entry name" value="zinc finger protein 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002402">
        <property type="entry name" value="Zinc finger protein 347"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000281">
        <property type="entry name" value="Zinc finger protein 558 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000877">
        <property type="entry name" value="zinc finger protein 599"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000099">
        <property type="entry name" value="Zinc finger protein 79"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="Q."/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
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                <person name="J."/>
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                <person name="M."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        </source>
    </evidence>
    <sequence length="576" mass="64168" checksum="67B49D2E52D27566" modified="2023-09-13" version="1">MIVFGWAVFLVSRSLGQGLLWTLGEHIARFLGTRGAAATMGNSCICRDDSGAEDSVDAQQQQAENSAVPTAGTRSQPRNPVRPPRRGRGPHEPRRKKQNVDGLVLDTLAVIRTLVDNDQEPPYSMITLHEMAETDEGWLDVVQSLIRVIPLEDPLGPAVITLLLDECPLPTKEALQKLTEILNLNGEVACQDSGHPAKHRNTSAVLGCLAEKLAGPASIGLFSPGILEYLLQCLKLQSHPTVMLFALIALEKFAQTSENKLTISESSISDRLVTLESWADDPDYLKRQVGFCAQWSLDNLFLKEGRQLTYEKVDLNSIRTMLNSNDVSEYLKISPHGLEARCDASSFESVRCTFCVDAGVWYYEVTVVTSGVMQIGWATRASKFLNHEGYGIGDDEYSCAYDGCRQLIWYNARSKPHLHPCWKEGDTVGFLLDLDEKQMIFFLNGNQLPPENQVFSSTVSGFFAAASFMSYQQCEFNFGAKPFKYPPSTKFSTFNDYAFLTAEEKIILPRHRRLALLKQVSIRENCCSLCCDEVADTQLKPCGHSDLCMDCALQLETCPLCRKEIVSRVRQISHIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JNL4</accession>
    <name>A0A9L0JNL4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ERI1 exoribonuclease 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Exonuclease domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106839003</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ERI2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014708917.1">
        <property type="nucleotide sequence ID" value="XM_014853431.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JNL4"/>
    <dbReference type="Ensembl" id="ENSEAST00005080477.1">
        <property type="protein sequence ID" value="ENSEASP00005050755.1"/>
        <property type="gene ID" value="ENSEASG00005031839.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106839003"/>
    <dbReference type="KEGG" id="eai:106839003"/>
    <dbReference type="GeneTree" id="ENSGT00530000063205"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000175">
        <property type="term" value="F:3'-5'-RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06133">
        <property type="entry name" value="ERI-1_3'hExo_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000062">
        <property type="entry name" value="ERI1 exoribonuclease 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051274">
        <property type="entry name" value="3-5_Exoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047201">
        <property type="entry name" value="ERI-1_3'hExo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013520">
        <property type="entry name" value="Ribonucl_H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010666">
        <property type="entry name" value="Znf_GRF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044">
        <property type="entry name" value="3'-5' EXONUCLEASE ERI1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23044:SF61">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00929">
        <property type="entry name" value="RNase_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06839">
        <property type="entry name" value="Zn_ribbon_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00479">
        <property type="entry name" value="EXOIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51999">
        <property type="entry name" value="ZF_GRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="GRF-type" evidence="14">
        <location>
            <begin position="595"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="528"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="556"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="689" mass="76834" checksum="5B4E6863A3223344" modified="2023-09-13" version="1">MATKRLARQLGLIRRKSIVPANGNLGSSKSKQLFDYLIVIDFESTCWNDGKHHQSQEIIEFPAVLLNTSTGEIESEFHAYVQPQEHPILSEFCMELTGIKQAQVDEGVPLKICLSQFCKWIQKIQQQKKITFASGVADLSTSEVKLCAFVTWSDWDLGVCLEYECKRKQLLKPVFLNSWIDLRVTYKIFYKRKPKGLSGALQEVGIEFLGREHSGLDDSRNTALLAWKMIRDGCFMKITRPLKKVPTKKNPNILARNLNTNQVEETSACNSNTQGPSIFDSEPKNAINPHEKVKVTSVGVNPPIMVQQDQLQLKNDIKVDLHNVKSCFSLFNTKSSTSLGQLQSPSLDTPMQKQIKNERLAFNTKSKSSAIGSELVRVSTTLSSVNHVSDTEMSSAFHCLPMLADWEDIALLPASQPEQSVDFMPAISDSNLDTSFNSGERLMVLKESEMLSHENLGGTEETPQKSETSKSIVYKSPHTTIYDVKEPKNPGSDVSDFKLPECKSSSFNSVNASMSHLSVLGKHPLLLGGTERSPSVPSAFPPAKKQTFTSHEEKPTSSLGSPGRRSSQKVLPSVLTSTVNLREPWKSGRMTPPLCKCGRRCKRLVVSNNGPNHGKVFYCCPIGKYQENRKCCGYFKWEQTLQKERANSIVQPHSPGGLAFSSPEISPICDRNVNFSTENSLRLRPSMRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JR39</accession>
    <name>A0A9L0JR39_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Immunity related GTPase cinema</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">IRGC</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JR39"/>
    <dbReference type="Ensembl" id="ENSEAST00005021400.2">
        <property type="protein sequence ID" value="ENSEASP00005055751.1"/>
        <property type="gene ID" value="ENSEASG00005027388.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005053288.1">
        <property type="protein sequence ID" value="ENSEASP00005045502.1"/>
        <property type="gene ID" value="ENSEASG00005027388.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005074182.1">
        <property type="protein sequence ID" value="ENSEASP00005045646.1"/>
        <property type="gene ID" value="ENSEASG00005027388.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005075549.1">
        <property type="protein sequence ID" value="ENSEASP00005062555.1"/>
        <property type="gene ID" value="ENSEASG00005027388.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005080165.1">
        <property type="protein sequence ID" value="ENSEASP00005055791.1"/>
        <property type="gene ID" value="ENSEASG00005027388.1"/>
    </dbReference>
    <dbReference type="KEGG" id="eai:106848434"/>
    <dbReference type="GeneTree" id="ENSGT00950000183007"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097226">
        <property type="term" value="C:sperm mitochondrial sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04104">
        <property type="entry name" value="p47_IIGP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000541">
        <property type="entry name" value="Immunity related GTPase M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030385">
        <property type="entry name" value="G_IRG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007743">
        <property type="entry name" value="Immunity-related_GTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051515">
        <property type="entry name" value="IRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="PANTHER" id="PTHR32341">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR32341:SF10">
        <property type="entry name" value="INTERFERON-INDUCIBLE GTPASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05049">
        <property type="entry name" value="IIGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51716">
        <property type="entry name" value="G_IRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IRG-type G" evidence="6">
        <location>
            <begin position="53"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="405"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005055791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="464" mass="50649" checksum="DF3E9A6BF31C81F0" modified="2023-09-13" version="1">MATSKLPAVPREEETTILMAKEELEALRTAFESGDIPQAASRLRELLASSESTRLEVGVTGESGAGKSSLINALRGLGAEDPGAALTGVVETTMHPSPYPHPQFPDVTLWDLPGAGSPGCPADKYLKQVDFGRYDFFLLVSPRRCGAVETRLASEILRQGKKFYFVRTKVDEDLAATRTQRPSGFSEAAVLQEIREHCAERLRVAGVTDPRIFLVSNLSPVRYDFPLLVSTWEHDLPAHRRHAGLLSLPDISLEALQKKKDMLQEQVLKTALVSGVIQALPVPGLAAAYDDALLIRSLRGYHRSFGLDDDSLAKLAEQVGKQAGDLRSVIRSPLANELSPETILRLYSRSSDGAMRVARAFEKGIPVFGTLVAGGISFGTVYTMLQGCLNEMAEDAQRVRIKALEEDEPQSEVSLEAAGDNGVEKRASGEGVSEEAPLSARRKLGLLLKYILESWKKRDLSEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JR70</accession>
    <name>A0A9L0JR70_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Potassium inwardly rectifying channel subfamily J member 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">KCNJ8</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014687939.1">
        <property type="nucleotide sequence ID" value="XM_014832453.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JR70"/>
    <dbReference type="SMR" id="A0A9L0JR70"/>
    <dbReference type="Ensembl" id="ENSEAST00005039405.1">
        <property type="protein sequence ID" value="ENSEASP00005052482.1"/>
        <property type="gene ID" value="ENSEASG00005031838.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106825577"/>
    <dbReference type="KEGG" id="eai:106825577"/>
    <dbReference type="CTD" id="3764"/>
    <dbReference type="GeneTree" id="ENSGT01140000282491"/>
    <dbReference type="OMA" id="RMITEHC"/>
    <dbReference type="OrthoDB" id="273257at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034702">
        <property type="term" value="C:monoatomic ion channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030016">
        <property type="term" value="C:myofibril"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015272">
        <property type="term" value="F:ATP-activated inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099508">
        <property type="term" value="F:voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902282">
        <property type="term" value="F:voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060922">
        <property type="term" value="P:atrioventricular node cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070588">
        <property type="term" value="P:calcium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061762">
        <property type="term" value="P:CAMKK-AMPK signaling cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060976">
        <property type="term" value="P:coronary vasculature development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008340">
        <property type="term" value="P:determination of adult lifespan"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030010">
        <property type="term" value="P:establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045444">
        <property type="term" value="P:fat cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015908">
        <property type="term" value="P:fatty acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048144">
        <property type="term" value="P:fibroblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061535">
        <property type="term" value="P:glutamate secretion, neurotransmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003007">
        <property type="term" value="P:heart morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001774">
        <property type="term" value="P:microglial cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050905">
        <property type="term" value="P:neuromuscular process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044546">
        <property type="term" value="P:NLRP3 inflammasome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038066">
        <property type="term" value="P:p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150103">
        <property type="term" value="P:reactive gliosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008217">
        <property type="term" value="P:regulation of blood pressure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002027">
        <property type="term" value="P:regulation of heart rate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033198">
        <property type="term" value="P:response to ATP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034097">
        <property type="term" value="P:response to cytokine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034976">
        <property type="term" value="P:response to endoplasmic reticulum stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043330">
        <property type="term" value="P:response to exogenous dsRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001666">
        <property type="term" value="P:response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032868">
        <property type="term" value="P:response to insulin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002931">
        <property type="term" value="P:response to ischemia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032496">
        <property type="term" value="P:response to lipopolysaccharide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904638">
        <property type="term" value="P:response to resveratrol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009410">
        <property type="term" value="P:response to xenobiotic stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051124">
        <property type="term" value="P:synaptic assembly at neuromuscular junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019226">
        <property type="term" value="P:transmission of nerve impulse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042311">
        <property type="term" value="P:vasodilation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003229">
        <property type="term" value="P:ventricular cardiac muscle tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000050">
        <property type="entry name" value="ATP-sensitive inward rectifier potassium channel 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000001">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003278">
        <property type="entry name" value="K_chnl_inward-rec_Kir6.1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF11">
        <property type="entry name" value="ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005465">
        <property type="entry name" value="GIRK_kir"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01331">
        <property type="entry name" value="KIR61CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9 14" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
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    <keyword evidence="7 14" id="KW-0630">Potassium</keyword>
    <keyword evidence="3 14" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 14" id="KW-0813">Transport</keyword>
    <keyword evidence="6 14" id="KW-0851">Voltage-gated channel</keyword>
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        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="154"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="17">
        <location>
            <begin position="37"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="18">
        <location>
            <begin position="191"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="375"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="387"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="site" description="Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium" evidence="13">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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    </evidence>
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            <dbReference type="RuleBase" id="RU003822"/>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF01007"/>
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            <dbReference type="Pfam" id="PF17655"/>
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            <dbReference type="Ensembl" id="ENSEASP00005052482.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="424" mass="47936" checksum="376EAD31C0959F2A" modified="2023-09-13" version="1">MLARKSIIPEEYVLARIAAENLRKPRIRDRLPKARFIAKSGACNLAHKNIREQGRFLQDIFTTLVDLKWRHTLVIFTMSFLCSWLLFAIMWWLVAFAHGDIYAYMEKSGMEKSGLESTVCVTNVRSFTSAFLFSIEVQVTIGFGGRMMTEECPLAITVLILQNIVGLIINAVMLGCIFMKTAQAHRRAETLIFSRHAVIAVRNGKLCFMFRVGDLRKSMIISASVRIQVVKKTTTPEGEVVPIHQLDIPVDNPIESNNIFLVAPLIICHVIDKRSPLYDISASDLANQDLEVIVILEGVVETTGITTQARTSYIAEEIQWGHRFVSIVTEEEGVYSVDYSKFGNTVKVAAPRCSARELDEKPSILIQTLQKSELSHQNSLRKRNSMRRNNSMRRNNSIRRNNSSLIVPKVQFMTPEGNQNTSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JS32</accession>
    <name>A0A9L0JS32_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">ATP synthase F(0) complex subunit f, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">ATP synthase membrane subunit f</fullName>
        </alternativeName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
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                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Subunit f, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Component of the ATP synthase complex composed at least of ATP5F1A/subunit alpha, ATP5F1B/subunit beta, ATP5MC1/subunit c (homooctomer), MT-ATP6/subunit a, MT-ATP8/subunit 8, ATP5ME/subunit e, ATP5MF/subunit f, ATP5MG/subunit g, ATP5MK/subunit k, ATP5MJ/subunit j, ATP5F1C/subunit gamma, ATP5F1D/subunit delta, ATP5F1E/subunit epsilon, ATP5PF/subunit F6, ATP5PB/subunit b, ATP5PD/subunit d, ATP5PO/subunit OSCP. ATP synthase complex consists of a soluble F(1) head domain (subunits alpha(3) and beta(3)) - the catalytic core - and a membrane F(0) domain - the membrane proton channel (subunits c, a, 8, e, f, g, k and j). These two domains are linked by a central stalk (subunits gamma, delta, and epsilon) rotating inside the F1 region and a stationary peripheral stalk (subunits F6, b, d, and OSCP).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase F chain family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JS32"/>
    <dbReference type="Ensembl" id="ENSEAST00005058002.1">
        <property type="protein sequence ID" value="ENSEASP00005055999.1"/>
        <property type="gene ID" value="ENSEASG00005034763.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00510000046986"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019344">
        <property type="entry name" value="F1F0-ATPsyn_F_prd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13080">
        <property type="entry name" value="ATP SYNTHASE F CHAIN, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13080:SF16">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT F, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10206">
        <property type="entry name" value="WRW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4" id="KW-0138">CF(0)</keyword>
    <keyword evidence="7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005895"/>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="87" mass="10188" checksum="C3FF0A5AB3C31E90" modified="2023-09-13" version="1">MDVKIGELPSWKVMWDFTPKGIVGAFQRGYCCYYDKYEQVCQCEERGRCWGFYGAYVLFNYCLSCKELKCERPHKHHQGETSVDGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JY59</accession>
    <name>A0A9L0JY59_EQUAS</name>
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            <fullName evidence="3">Inhibin beta A chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Activin beta-A chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">INHBA</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Inhibin A is a dimer of alpha/INHA and beta-A/INHBA that functions as a feedback regulator in the hypothalamic-pituitary-gonadal (HPG) axis. Inhibits the secretion of FSH from the anterior pituitary gland by acting on pituitary gonadotrope cells. Antagonizes activin A by binding to the proteoglycan, betaglycan, and forming a stable complex with and, thereby, sequestering type II activin receptors while excluding type I receptor.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Dimeric, linked by one or more disulfide bonds. Inhibin A is a dimer of alpha/INHA and beta-A/INHBA. Activin A is a homodimer of beta-A/INHBA. Activin AB is a dimer of beta-A/INHBA and beta-B/INHBB. Interacts with FST and FSTL3; these interactions prevent activin A interaction to its type II receptor. Activin A interacts with ACVR2A. Activin A interacts with BMPR2. Inhibin A interacts with ACVR1; this interaction creates a non-signaling complex (NSC) that inhibits ACVR1-mediated BMP signaling. Inhibin A interacts with ACVR2A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the TGF-beta family.</text>
    </comment>
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        <property type="component" value="Chromosome 1"/>
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        <property type="term" value="C:activin A complex"/>
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        <property type="term" value="C:enzyme activator complex"/>
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        <property type="term" value="F:cytokine activity"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:peptide hormone binding"/>
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        <property type="term" value="P:eyelid development in camera-type eye"/>
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        <property type="term" value="P:hemoglobin biosynthetic process"/>
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        <property type="term" value="P:positive regulation of extrinsic apoptotic signaling pathway in absence of ligand"/>
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        <property type="entry name" value="TGF-b_C"/>
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        <property type="entry name" value="TGF-b_propeptide"/>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
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    <name>A0A9L0JYC7_EQUAS</name>
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            <fullName evidence="26">Histone-lysine N-methyltransferase NSD3</fullName>
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        <alternativeName>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Laurasiatheria</taxon>
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        </lineage>
    </organism>
    <reference evidence="36 37" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="36" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="36" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="22">Histone methyltransferase. Preferentially dimethylates 'Lys-4' and 'Lys-27' of histone H3 forming H3K4me2 and H3K27me2. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-lysyl(27)-[histone H3] + 2 S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl(27)-[histone H3] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
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            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61976"/>
            <dbReference type="EC" id="2.1.1.371"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>L-lysyl(4)-[histone H3] + 2 S-adenosyl-L-methionine = N(6),N(6)-dimethyl-L-lysyl(4)-[histone H3] + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
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    </comment>
    <comment type="subunit">
        <text evidence="23">Interacts with BRD4. Interacts (via KIKL motif) with BRD3 (via NET domain).</text>
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        <subcellularLocation>
            <location evidence="2">Chromosome</location>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00050891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00052270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00056539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00065976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00066818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00066819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00068930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00081740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="35">
        <source>
            <dbReference type="PROSITE" id="PS51215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005058144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="37">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1438" mass="161582" checksum="B9E149382F89AC80" modified="2023-09-13" version="1">MDFSFSFMQGIMGNTIQQPPQLIDSANIRQEDAFDNNSDIVEDGGQTPYEATLQQGFQYPPTTEDLPPLTNGYPPSISMYETQTKYQSYNQYPNGSANGFGAVRNFSPADYYHSDIPNTRPHEILEKPSPPQPPPPPSVPQTVIPKKTGSPEIKLKITKTIQNGRELFESSLCGDLLNEVQASEHTKSKHESRKEKRKKSNKHDSSRSEERKSHKIPKLEPEEQNRPNERVDTVSEKPREDLVLKEETPVQPILSSVPTTEVSTGVKFQVGDLVWSKVGTYPWWPCMVSSDPQLEVHTKINTRGAREYHVQFFSNQPERAWVHEKRVREYKGHKQYEELLAEATKQASNHSEKQKIRKPRPQRERAQWDIGIAHAEKALKMTREERIEQYTFIYIDKQPEEALSQAKKNVASKAEVKKTRRPRSVLNTQPEQTNAGEVASSLSSTEIRRHSQRRHMSVEEEEPPPVKIAWKTAAARKSLPASITMHKGSLDLQKCNMSPVVKIEQVFALQNATGDGKFIDQFVYSTKGIGNKTEISVRGQDRLIISTPNQRNEKATQNISSPETTSGSTGSAEKKQQRRSIRTRSESEKSTEVVPKKKIKKEQVETVPQATVKTGLQKGASEISDSCKPLKKRSRASTDVEMTSSAYRDTSDSDSRGLSDLQVGFGKQVDSPSATAEADVSDVQSVDSSLSRRGIGMSKKDAVCQVCESSGDSLIPCEGECCKHFHLECLGLASLPDGKFVCMECKTGQHPCFSCKMSGTDVKRCSVGACGKFYHEACVRKFPTAIFESKGFRCPQHCCSACSMEKDVHKASKGRMMRCLRCPVAYHTGDACIAAGSMSVSSCIFICSNHSKRSSNSSSAVNVGFCFVCARGLIVQDHSDPMFSSYAYKSHYLLNESNRAELMKLPMIPSSSASKKKCEKGGRLLCCESCPASFHPECLSIEMPEGCWNCNDCKAGKKLHYKQIVWVKLGNYRWWPAEICNPRSVPLNIQGLKHDLGDFPVFFFGSHDYYWVHQGRVFPYVEGDKSFAEGQTSINKTFKKALEEAAKRFQELKAQRESKEALEIEKSSRKPPPYKHIKANKVIGKVQIQVADLSEIPRCNCKPADENPCGLESECLNRMLQYECHPQVCPAGDRCQNQCFTKRLYPDAEIIKTERRGWGLRTKRSIKKGEFVNEYVGELIDEEECRLRIKRAHENSVTNFYMLTVTKDRIIDAGPKGNYSRFMNHSCNPNCETQKWTVNGDVRVGLFALCDIPAGMELTFNYNLDCLGNGRTECHCGADNCSGFLGVRPKSACASTTEEKAKNAKLKQKRRKIKTEPKQMHEDYCFQCGDGGELVMCDKKDCPKAYHLLCLNLTQPPYGKWECPWHQCNECSSAAVSFCEFCPRSFCKDHEKGVLVPSALEGRLCCSEHDPVSPVSSEYWSKIKCKLESQDPGEEVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K4Q9</accession>
    <name>A0A9L0K4Q9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphodiesterase</fullName>
            <ecNumber evidence="10">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PDE7B</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.4.53"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_014706075.1">
        <property type="nucleotide sequence ID" value="XM_014850589.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K4Q9"/>
    <dbReference type="Ensembl" id="ENSEAST00005059986.1">
        <property type="protein sequence ID" value="ENSEASP00005060466.1"/>
        <property type="gene ID" value="ENSEASG00005011165.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837186"/>
    <dbReference type="CTD" id="27115"/>
    <dbReference type="GeneTree" id="ENSGT00940000159413"/>
    <dbReference type="OrthoDB" id="189220at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000004">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0114">cAMP</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="12">
        <location>
            <begin position="149"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="19"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="468"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="475"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="490"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="225"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="225"/>
            <end position="229"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="375"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="426"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00061458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005060466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="503" mass="57309" checksum="4D109594A35A23EA" modified="2023-09-13" version="1">MPVLERYFPSAELGRRWTAPEGVLPSSPGGRPGCQQGPLPWDSPEMIRMVKLVWKSKSELQATKQRGVLETEDALHGFPGDVRLRGQTGVPAERRGSYPFIDFRPLNNTTHSGEIGTKKKVKRLLSFQRYFHASRLLRGIVPQAPLHLLDEDYLGQARHMLSKVGMWDFDIFLFDRLTNGNSLVTLLCHLFSTHGLIHHFKLDMVTLHRFLVMVQEDYHSQNPYHNAVHAADVTQAMHCYLKEPKLASFLTPLDIMLGLLAAAAHDVDHPGVNQPFLIKTNHHLANLYQNMSVLENHHWRSTIGMLRESRLLAHLPKEMTQDIEQQLGSLILATDINRQNEFLARLRTHLHNKDLRLEDGRDRHFMLQIALKCADICNPCRVWEMSKQWSERVCEEFYRQGDLEQKFELEISPLCNQQKDSIPSIQIGFMTYIVEPLFREWARFTGNSTLSENMLSHLAHNKAQWKSLLPKPHRGGSGGGGGPDLAGQGAEKEEQTGTRGDTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K571</accession>
    <name>A0A9L0K571_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major allergen I polypeptide chain 1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106829201</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Secreted</location>
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    <comment type="similarity">
        <text evidence="2">Belongs to the secretoglobin family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Androgen-binding protein"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="CH1-like"/>
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    <dbReference type="InterPro" id="IPR035960">
        <property type="entry name" value="Secretoglobin_sf"/>
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        <property type="entry name" value="ABPA10-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ABPA10-RELATED"/>
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        <property type="entry name" value="Uteroglobin"/>
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        <property type="entry name" value="FELALLERGEN"/>
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        <property type="entry name" value="Uteroglobin-like"/>
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        <property type="entry name" value="SCGB"/>
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        <location>
            <begin position="1"/>
            <end position="22"/>
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        <location>
            <begin position="23"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005060626.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Yang"/>
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                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="Pan"/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="C."/>
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                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Cell membrane</location>
            <topology evidence="2 15">Multi-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
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    <dbReference type="GeneTree" id="ENSGT00940000162891"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="7tmA_OR11G-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF218">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="29"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="64"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="103"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
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    <feature type="transmembrane region" description="Helical" evidence="15">
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    <feature type="transmembrane region" description="Helical" evidence="15">
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    <feature type="transmembrane region" description="Helical" evidence="15">
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    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="45"/>
            <end position="294"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005062471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="311" mass="34953" checksum="964D78847FA81B1C" modified="2023-09-13" version="1">MKNSNILNNFSNITGFILLGFPCPREGQILLFVLFSVVYLLTLMGNGSIICAVRWDQRLHTPMYILLANFSFLEICYVTSTVPNMLANFLSDTKVISFSGCFVQFYFFFSLGSTECFFLAIMAFDRYLAICWPLRYPTLMTGRLCTNLVVSCWVLGFLWFPVPIIIISQMSFCGSKIIDHFLCDPGPLLALACARAPVMEFYWTFISSLLLFMPFLCIMGSYALLLRAVFSLPSAAGRRKAFSTCGSHLAVVSLFYGSVMVMYLSPASEHEAGMQKLVTLFYSVGTPLINPVIYSLRNKDMKHALQKLMGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KJP0</accession>
    <name>A0A9L0KJP0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Mitochondrial glycine transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Solute carrier family 25 member 38</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 15" type="primary">SLC25A38</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Mitochondrial glycine transporter that imports glycine into the mitochondrial matrix. Plays an important role in providing glycine for the first enzymatic step in heme biosynthesis, the condensation of glycine with succinyl-CoA to produce 5-aminolevulinate (ALA) in the miochondrial matrix. Required during erythropoiesis.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Plays a role as pro-apoptotic protein that induces caspase-dependent apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>glycine(in) = glycine(out)</text>
            <dbReference type="Rhea" id="RHEA:70715"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="11">Mitochondrion inner membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the mitochondrial carrier (TC 2.A.29) family. SLC25A38 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KJP0"/>
    <dbReference type="Ensembl" id="ENSEAST00005062411.1">
        <property type="protein sequence ID" value="ENSEASP00005061930.1"/>
        <property type="gene ID" value="ENSEASG00005007597.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075117"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015187">
        <property type="term" value="F:glycine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030218">
        <property type="term" value="P:erythrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904983">
        <property type="term" value="P:glycine import into mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000100">
        <property type="entry name" value="Mitochondrial glycine transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000118">
        <property type="entry name" value="Mitochondrial glycine transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03064">
        <property type="entry name" value="SLC25A38"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030847">
        <property type="entry name" value="Hem25/SLC25A38"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46181">
        <property type="entry name" value="MITOCHONDRIAL GLYCINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46181:SF3">
        <property type="entry name" value="MITOCHONDRIAL GLYCINE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 11 13" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="25"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="121"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="12">
        <location>
            <begin position="215"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="304" mass="33647" checksum="99B970D0355B91CD" modified="2023-09-13" version="1">MIQKSRPALLQPQDVGDRVETLMLHPVIKAFLCGSVSGTCSTLLFQPLDLLKTRLQTLQPSPHGSRRIGMLALLLKVVRTESLLGLWKGMSPSIVRCVPGVGIYFGTLYSLKQYFLRGHPPTALESVILGVGSRSVAGVCMSPITVIKTRYESGRYGYESIHAALRSIYRNEGHRGLFSGLTATLLRDAPFSGIYLMFYNQTKNIMLHDQLDVAFLPVVNFSCGIFAGILASLVTQPADVIKTHMQLSPVKFRWIGQAVTLIFKDYGLRGFFQGGVPRALRRTLMAAMAWTVYEEMMAKMGLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KJY5</accession>
    <name>A0A9L0KJY5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">POU3F2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the POU transcription factor family. Class-3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KJY5"/>
    <dbReference type="Ensembl" id="ENSEAST00005055391.1">
        <property type="protein sequence ID" value="ENSEASP00005062040.1"/>
        <property type="gene ID" value="ENSEASG00005031507.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164385"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000005">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000001">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016362">
        <property type="entry name" value="TF_POU_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF115">
        <property type="entry name" value="POU DOMAIN, CLASS 3, TRANSCRIPTION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00157">
        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
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    <dbReference type="PIRSF" id="PIRSF002629">
        <property type="entry name" value="Transcription_factor_POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00028">
        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00352">
        <property type="entry name" value="POU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0371">Homeobox</keyword>
    <keyword evidence="7 8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="13">
        <location>
            <begin position="173"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="263"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
            <begin position="265"/>
            <end position="324"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="20"/>
            <end position="115"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="144"/>
            <end position="178"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="11">
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            <begin position="82"/>
            <end position="93"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="11">
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            <begin position="105"/>
            <end position="115"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
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            <dbReference type="ARBA" id="ARBA00010250"/>
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    <evidence type="ECO:0000256" key="3">
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            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
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    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
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        <source>
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        <source>
            <dbReference type="RuleBase" id="RU361194"/>
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    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005062040.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="349" mass="37417" checksum="49EB19A50467E81C" modified="2023-09-13" version="1">MATAASNHYSLLTSSASIVHAEPPGGMQQGAGGYREAQSLVQGDYGALQSNGHPLSHRGGGGGDGSPWSTSPLGQPDIKPSQQQQQQQQQQQQRPPHLVHHAANHHPGPGAWRSAAAAAHLPPSMGASNGGLLYSQPSFTVNGMLGAGGQPAGLHHHGPPGHPGAHHDPHSDEDTPTSDDLEQFAKQFKQRRIKLGFTQADVGLALGTLYGNVFSQTTICRFEALQLSFKNMCKLKPLLNKWLEEADSSSGSPTSIDKIAAQGRKRKKRTSIEVSVKGALESHFLKCPKPSAQEITSLADSLQLEKEVVRVWFCNRRQKEKRMTPPGGTLPGAEDVYGGSRDTRCNDMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KRB3</accession>
    <name>A0A8C4KRB3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tubulin beta chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TUBB4A</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KRB3"/>
    <dbReference type="Ensembl" id="ENSEAST00005000400.2">
        <property type="protein sequence ID" value="ENSEASP00005000318.2"/>
        <property type="gene ID" value="ENSEASG00005027092.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161972"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005930">
        <property type="term" value="C:axoneme"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045171">
        <property type="term" value="C:intercellular bridge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033269">
        <property type="term" value="C:internode region of axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043209">
        <property type="term" value="C:myelin sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005200">
        <property type="term" value="F:structural constituent of cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031115">
        <property type="term" value="P:negative regulation of microtubule polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd02187">
        <property type="entry name" value="beta_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000002">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000003">
        <property type="entry name" value="Tubulin beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002453">
        <property type="entry name" value="Beta_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01163">
        <property type="entry name" value="BETATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="14">
        <location>
            <begin position="106"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="15">
        <location>
            <begin position="305"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000318.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="503" mass="56284" checksum="DD2466E13C1F7C24" modified="2023-09-13" version="2">MSSSPMSALSLDVRPQRRPRGAGTVLLRRRRRRRVHHQPRRRSPSIRIPAYASPTSATAMREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLERINVYYNEATGGNYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDAVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEFPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLSVQSKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEGEFEEEAEEEVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KWC6</accession>
    <name>A0A8C4KWC6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">EGF domain-specific O-linked N-acetylglucosamine transferase</fullName>
            <ecNumber evidence="2">2.4.1.255</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Extracellular O-linked N-acetylglucosamine transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">EOGT</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in extracellular proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). Specifically glycosylates the Thr residue located between the fifth and sixth conserved cysteines of folded EGF-like domains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl)-L-seryl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48904"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:12251"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:90838"/>
            <dbReference type="EC" id="2.4.1.255"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonyl-[protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48908"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:12252"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:90840"/>
            <dbReference type="EC" id="2.4.1.255"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 61 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.255" evidence="2"/>
    <dbReference type="RefSeq" id="XP_014691568.1">
        <property type="nucleotide sequence ID" value="XM_014836082.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044610547.1">
        <property type="nucleotide sequence ID" value="XM_044754612.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KWC6"/>
    <dbReference type="Ensembl" id="ENSEAST00005002768.2">
        <property type="protein sequence ID" value="ENSEASP00005002518.1"/>
        <property type="gene ID" value="ENSEASG00005001959.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106827921"/>
    <dbReference type="KEGG" id="eai:106827921"/>
    <dbReference type="CTD" id="285203"/>
    <dbReference type="GeneTree" id="ENSGT00940000156493"/>
    <dbReference type="OMA" id="GHCELNR"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097363">
        <property type="term" value="F:protein O-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049625">
        <property type="entry name" value="Glyco_transf_61_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007657">
        <property type="entry name" value="Glycosyltransferase_61"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961:SF148">
        <property type="entry name" value="EGF DOMAIN-SPECIFIC O-LINKED N-ACETYLGLUCOSAMINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20961">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04577">
        <property type="entry name" value="Glyco_transf_61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034205579" description="EGF domain-specific O-linked N-acetylglucosamine transferase" evidence="13">
        <location>
            <begin position="21"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 61 catalytic" evidence="14">
        <location>
            <begin position="338"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005002518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="527" mass="61629" checksum="6A20555BADF83C05" modified="2022-01-19" version="1">MLMLLVFGVLLHDIPLSGQAEASPEADGIPGEPLFNYASIRLPEEHIPFFLHNNGHIATVCKKDSHCPYKKHLENLKYCWGYEKSCKPEFRFGYPVCTYVDMGWTDTLESAQDIFWKQADFGYAGERLEELHVLCQPKEANDSSLVCSRYLQYCRAANLYLDLRNIQRNHDRFKEDFFQSGEIGGHCKLDIRTLMSEGQRKSPLQSWFAELQSYTQLPFRPIEDAKCDIVIEKPTYFMKLDAGVNMYHHFCDFINLYITQHVNNSFSTDVHIVMWDTSSYGYGDLFSDTWKAFTDYDVIHLKTYDSKRVCFKEAVFSLLPRMRYGLFYNTPLISGCQNTGLFRAFSQHVLHRLNITQEGPKDGKIRVTILARSTEYRKILNQNELVNALKTVSTFEVQIVDYKYKQLGFLDQLRITHNTDIFIGMHGAGLTHLLFLPDWAAVFELYNCEDERCYLDLARLRGVHYITWRRQNKVFPQDKGHHPTLGEHPKFTNYSFDVEEFMYLVLQAADYVLQHPKWPFKKKRDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KZD1</accession>
    <name>A0A8C4KZD1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein AAR2 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">AAR2 splicing factor homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">AAR2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the U5 snRNP complex that is required for spliceosome assembly and for pre-mRNA splicing.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with PRPF8 (via RNase H homology domain). Component of a U5 snRNP complex that contains PRPF8.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AAR2 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014706289.1">
        <property type="nucleotide sequence ID" value="XM_014850803.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014706290.1">
        <property type="nucleotide sequence ID" value="XM_014850804.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014706291.1">
        <property type="nucleotide sequence ID" value="XM_014850805.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044604189.1">
        <property type="nucleotide sequence ID" value="XM_044748254.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KZD1"/>
    <dbReference type="SMR" id="A0A8C4KZD1"/>
    <dbReference type="Ensembl" id="ENSEAST00005004931.2">
        <property type="protein sequence ID" value="ENSEASP00005004493.1"/>
        <property type="gene ID" value="ENSEASG00005003415.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837293"/>
    <dbReference type="KEGG" id="eai:106837293"/>
    <dbReference type="CTD" id="25980"/>
    <dbReference type="GeneTree" id="ENSGT00390000007796"/>
    <dbReference type="OMA" id="VWQSGGL"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000244">
        <property type="term" value="P:spliceosomal tri-snRNP complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13778">
        <property type="entry name" value="Aar2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13777">
        <property type="entry name" value="Aar2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.550:FF:000001">
        <property type="entry name" value="AAR2 splicing factor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.20:FF:000001">
        <property type="entry name" value="protein AAR2 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
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                <person name="Wang"/>
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                <person name="J."/>
                <person name="Li"/>
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                <person name="Yin"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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</entry>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Fang"/>
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                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="Ji"/>
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                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="Liu"/>
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                <person name="Xu"/>
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                <person name="Liu"/>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Mammalia</taxon>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
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                <person name="Wang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
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                <person name="G."/>
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                <person name="W."/>
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                <person name="J."/>
                <person name="Pan"/>
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                <person name="G."/>
                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
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                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
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                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
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                <person name="M."/>
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                <person name="D.D."/>
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                <person name="S."/>
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                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms a complex with RUVBL1 and RUVBL2. Part of a complex composed of SMG1, DHX34 and UPF1; within the complex DHX34 acts as a scaffolding protein to facilitate SMG1 phosphorylation of UPF1. Interacts with UPF1, MOV10, EIF4A3, XRN2, SMG6, SMG7, SMG9, UPF3A, UPF3B, CASC3/MLN51, XRN1, DIS3 and DCP1A; the interactions are RNA-independent. Interacts with NCBP1/CPB80; the interaction is RNA-dependent. Interacts (via C-terminus) with SMG1; the interaction is RNA-independent.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4L3A4"/>
    <dbReference type="Ensembl" id="ENSEAST00005006139.2">
        <property type="protein sequence ID" value="ENSEASP00005005617.1"/>
        <property type="gene ID" value="ENSEASG00005004155.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158721"/>
    <dbReference type="OMA" id="CIFANSP"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000623">
        <property type="term" value="P:negative regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042327">
        <property type="term" value="P:positive regulation of phosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd17979">
        <property type="entry name" value="DEXHc_DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000056">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000540">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000725">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056382">
        <property type="entry name" value="DHX34_Znf-C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF221">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24485">
        <property type="entry name" value="zf-C2H2_DHX34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="172"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="368"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="725"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="959"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1092"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="14">
        <location>
            <begin position="967"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1097"/>
            <end position="1107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00077457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00083360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1147" mass="128634" checksum="7CD122113431D57D" modified="2022-01-19" version="1">MPPPRTREGRDHRDRPRAPREKEAPEKWDWNCPETRRLFEDAFFRDEDYIRQGSEECQKFWTFFERLQKFQHLKTARKEEKDAGPPERSIPALADLPRAYDPRYRINLSVLGPDPRGSRGPGGRLPRDRVSEFRRALLHYLDFGQKQAFGRLAKLQRERAALPITQYASRILQTLERHQVVVVAGDTGCGKSTQVPQYLLAAGFSHVACTQPRRIACISLAKRVGFESLSQYGSQVGYQIRFESTRSPATKIVFLTVGLLLRQLQREPRLPQYQVLIVDEVHERHLHNDFLLGVLRRLLPQRPDLKVVLMSATINISLFSSYFGDAPVVQVPGRLFPITVVYQPQEAEPTTSKSEKLDPRPFLRVLEAIDSKYPPEERGDLLVFLSGMAEISAVLEAAQPYASHTQRWVVLPLHSALSVADQDKVFDVAPPGVRKCILSTNIAETSVTIDGIRFVVDSGKVKEMSCDPQAKLQRLQEFWISQASAEQRKGRAGRTGPGVCFRLYAESDYDAFAPYPVPEIRRVALDALVLQMKSMSVGDPRTFPFIEPPPPASLETAILYLRDQGALDSSEALTPIGSLLAQLPVDVVIGKMLILGSTFRLAEPVLTIAAALSVQSPFTRSAQGNPECAAARRPLESDQGDPFTLFNIFNAWVQVKCERSSNSRKWCRRRGIEEHRLYEMANLRRQFKELLEDHGLLAGAQALEPGDSYSRLRQRRERQALFQLKRQHEEGGARRRKVLRLGDQDGGSSDEDPARPASQGAGGSVDIQDVKFKLRHNLEQLRVAASAAQALSREQLALLKLVLARGLYPQLAAPDPFNSGRKDSDQIFHTQAKQGAVLHPTCVFASSPEVLHTREPEARGGEGSRDDKDKMSSRHQLLAFVSLLETTKPYLVNCVRVPALQSLLLFSRSLDTNGDCSRLVADGWLELQLADSESAVGLLAASLRLRARWEQVLARQLARQARRRPEEEEEDEDEEEAAVHRREEAALSRELLRFAASQVPYSLRRLMGLEIQNLYVGPQTITAAPSLPGLFGSSTLSPHPTKGGYVVTDFLTYNCLTSDTDLYSDCLRTFWTCPHCGLHAPLTPLERVAHENSCPQAPQDGAPGAEEAAPEPPQKASALQRPYHCEACQQDFLFTPTEVLRHRRQHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L419</accession>
    <name>A0A8C4L419_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Glutamate decarboxylase 1-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106842730</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the group II decarboxylase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L419"/>
    <dbReference type="Ensembl" id="ENSEAST00005003801.2">
        <property type="protein sequence ID" value="ENSEASP00005003468.1"/>
        <property type="gene ID" value="ENSEASG00005002683.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164469"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048786">
        <property type="term" value="C:presynaptic active zone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004351">
        <property type="term" value="F:glutamate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009449">
        <property type="term" value="P:gamma-aminobutyric acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06450">
        <property type="entry name" value="DOPA_deC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000016">
        <property type="entry name" value="Glutamate decarboxylase like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021115">
        <property type="entry name" value="Pyridoxal-P_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45677:SF6">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45677">
        <property type="entry name" value="GLUTAMATE DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00392">
        <property type="entry name" value="DDC_GAD_HDC_YDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 7 8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="556" mass="62710" checksum="D54223A3C5D12705" modified="2022-01-19" version="1">MRKKSVHTFESTEKDVKKDTRPENLMTNLSNAYASGMNLILGAGSIFFGTLLTDLVVYLFFPLDLLPSKDGQDLTESFLLHVVDILLHYIQKTFDVKSKVLDFHHPHQLLEGLDGFSLELSDHPESLEQLLVDCTDTLKYGVKTGHPRFLNQLSSGLDVIGLAGEWLTATANANMFTYEIAPVFTVMETILLKKMYEIIGWGETEGDGLFSPGGSISNLYGILTARYKQYPEIKRKGMTVLPGIVLFVSEQGHYSVKKAAAILGIGTDNVIEVKCDERGKMIPAELEKNILQAKRKGQTPFCVTATAGSTVLGAFDPLQDVADICERHNLWMHVDAAWGGGLLLSRNHSYKLSGIERANSVTWNPHKLMGVPLQCSAILIREKGLLEACNQMQAGYLFQPDKLYNVDFDTGDKTIQCGRHVDIFKLWLMWKAKGTSGFEAQINRYMELAKYFYKVLKKKDNFKLVLDAEPEFTNVCFWYFPPRLKHIPKGFERDQELQKIAPKIKAQMLEEGTAMISYQPCGDKVNFFRMVFSNPATRQTDVDYLIDEIERLGKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L536</accession>
    <name>A0A8C4L536_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exocyst complex component 3-like protein 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">EXOC3L4</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC6 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L536"/>
    <dbReference type="Ensembl" id="ENSEAST00005005987.2">
        <property type="protein sequence ID" value="ENSEASP00005005480.2"/>
        <property type="gene ID" value="ENSEASG00005004047.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234613"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
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        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051601">
        <property type="term" value="P:exocyst localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.70:FF:000006">
        <property type="entry name" value="Exocyst complex component 3 like 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.70">
        <property type="entry name" value="Exocyst complex component Sec6, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010326">
        <property type="entry name" value="EXOC3/Sec6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042532">
        <property type="entry name" value="EXOC3/Sec6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292:SF14">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 3-LIKE PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06046">
        <property type="entry name" value="Sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00070215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005480.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="728" mass="80144" checksum="35593DE91B6C8709" modified="2023-09-13" version="2">MPSPRTVTSGPELHSPKQPAEPQTPVQGTRRASSVDAPSTHHEDWRLGLNTFRRAFSRVSQGASGQAPEEDWGLLRSSSRFLFRSLRRALNDGPTADQSQATAVPGAAHGLDMPSRVMDGVSQRSSTGMGPEELEPKAGKSVADLIAQRQLLAAFEQLRHLETRLVAEKASRTFEQDPTGFARRAMDVCLHYDGLAAEIGAVVRETLGPDGVDAAALAELARVVRAEEEAHPAPPADGDFLLTPRRWRQHWEDAVRRSAQERVQLVGAGEAPGAAEGASGLPGLLDELGGLVRRDLQRVQREVQPAYAAAGFPVWEAYLRAFHAAVAQRLQELARDARGCEQLYVLLHWVANVYGSPDFLGTSDLALPAEPLPPLLAPEVWARLESDYASSLETKITSCFDGILQLEQSRWAASEASDVLQGRYHTPLSIDVHMLVAQQVKVSGAISAELEATTLRICARALGLFVPRFEKVCLESELLSEPHLGANINACEELRTSLLARFPGTFEELEKPLMVATCNFQKRLLQGLQRDVQPLFKVLCTRAWLTQDVLLPLMDKVVAFALHLEHVAPPWAQETLQEVHRYVIREYLAQVLRPRDRFRGIERMTASQKMGLEAQAIGDTFQGLGSEATWLGQAIPCVADILGETDKDNIQRHLETLIGSYPDIRQDHVLAILALRRLGRRRNQRLLQHAQNLLRVAAKAGGSGAAVGRVLFEEIEVPTSVDLLITWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L8C7</accession>
    <name>A0A8C4L8C7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Kinase</fullName>
            <ecNumber evidence="7">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ITPKC</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>1D-myo-inositol 1,4,5-trisphosphate + ATP = 1D-myo-inositol 1,3,4,5-tetrakisphosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11020"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57895"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.127"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:11021"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="7"/>
    <dbReference type="RefSeq" id="XP_014715767.1">
        <property type="nucleotide sequence ID" value="XM_014860281.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L8C7"/>
    <dbReference type="Ensembl" id="ENSEAST00005007891.2">
        <property type="protein sequence ID" value="ENSEASP00005007232.1"/>
        <property type="gene ID" value="ENSEASG00005005300.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106843300"/>
    <dbReference type="KEGG" id="eai:106843300"/>
    <dbReference type="CTD" id="80271"/>
    <dbReference type="GeneTree" id="ENSGT00940000160033"/>
    <dbReference type="OMA" id="WADNLWT"/>
    <dbReference type="OrthoDB" id="338650at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000828">
        <property type="term" value="F:inositol hexakisphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008440">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071277">
        <property type="term" value="P:cellular response to calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.160:FF:000001">
        <property type="entry name" value="Kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.160">
        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005522">
        <property type="entry name" value="IPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038286">
        <property type="entry name" value="IPK_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12400:SF106">
        <property type="entry name" value="INOSITOL-TRISPHOSPHATE 3-KINASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03770">
        <property type="entry name" value="IPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="191"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="317"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="60"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="96"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="239"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="666" mass="73856" checksum="7F10A8F9A5F5E2D1" modified="2022-01-19" version="1">MRRCPCRGSLSEAEAGALPGAARMGLEAPRGGRRRQPGQQRPGPGAGGPAGRPEGGGPRTRTEESSLHIEPERAGLGPEPGTDRQAEPKAAGFGAETERPSRKTEPDRSSLRTHLERSSRGSELETAGPWTETRTDGFWTNPHRSGLQPQPERARLWTEPGVDGPWTELEIHGSPTQLERAKPCTNLWTHQNRSSLWTQPERGCPSTEPSADGSWKELYTDGSRTQQDTEGPWTEPHTDGSQIQQDTETAWTQPGAHGPQMQDTHRPQTESWTDCLLGEPNQDDSLEEPEHGELVTHLYSHLESSSLTPVPRLIITPETPEPEAQPVGPPTRLEGGSGGFSSASSFDESEDDVVAGGGGASDPEDRSGNKPWKKLKTVLKYSPFMVSFRKHYPWVQLSGHAGNFQAGEDGRILKRFCQCEQHSLEQLMSDPLRPFVPAYYGTVQRDGQAFNQMEDLLADFDGPSIMDCKMGSRTYLEEELVKARERPRPRKDMYEKMVAVDPGAPTPEEHAQGAVTKPRYMQWRETVSSTSTLGFRIEGIKKANGTCNTNFKKTQEVEQVTKVLEDFVDGNHGLLRKYVARLEDLREALENSPFFKTHEVVGSSLLFVHDHTGLAKVWMIDFGKTVALPDHQTLSHRLPWAEGNREDGYLWGLDNMICLLQGLAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L8X6</accession>
    <name>A0A8C4L8X6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ribonuclease</fullName>
            <ecNumber evidence="11">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">RNASEH2A</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">The RNase H2 complex is a heterotrimer composed of the catalytic subunit RNASEH2A and the non-catalytic subunits RNASEH2B and RNASEH2C.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase HII family. Eukaryotic subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4L8X6"/>
    <dbReference type="Ensembl" id="ENSEAST00005006094.2">
        <property type="protein sequence ID" value="ENSEASP00005005576.2"/>
        <property type="gene ID" value="ENSEASG00005004118.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010768"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd07181">
        <property type="entry name" value="RNase_HII_eukaryota_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.460:FF:000001">
        <property type="entry name" value="Ribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000016">
        <property type="entry name" value="Ribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.460">
        <property type="entry name" value="Ribonuclease hii. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004649">
        <property type="entry name" value="RNase_H2_suA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023160">
        <property type="entry name" value="RNase_HII_hlx-loop-hlx_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00729">
        <property type="entry name" value="ribonuclease HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954:SF7">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-2" evidence="12">
        <location>
            <begin position="28"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005576.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="348" mass="38574" checksum="926718A3B60D0151" modified="2023-09-13" version="2">MDLSELERDNTGRCRLNSPVPAVCLKQPCALGVDEAGRGPVLGPMVYAICYCPLSRLADLEALKVADSKTLSESERDRLFARMKEDGDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTAAGLVQYALDQGVKVAQVFVDTVGMPETYQQRLQQRFPGIEVTVKAKADSLYPVVSAASICAKVARDQAVKNWKFVEKLQDSDTDYGSGYPNDPKTKAWLRRHVEPVFGFPQFVRFSWRTAQSILEKEAEDVVWDDSVAEDQEGVGRITAYFDEGLRSRTRLPHRVCSRKPGRHSLLHTWAACCESCGTAVLPAVRCRRKHHYAGHDSIAVLEAESPRSGCQRGGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LGC0</accession>
    <name>A0A8C4LGC0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Multicilin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Multiciliate differentiation and DNA synthesis-associated cell cycle protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Protein Idas</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">GMNN</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Transcription regulator specifically required for multiciliate cell differentiation. Acts in a multiprotein complex containing E2F4 and E2F5 that binds and activates genes required for centriole biogenesis. Required for the deuterosome-mediated acentriolar pathway. Plays a role in mitotic cell cycle progression by promoting cell cycle exit. Modulates GMNN activity by reducing its affinity for CDT1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterodimer (via coiled-coil domain) with GMNN (via coiled-coil domain); targets GMNN to the nucleus. Can form homodimers (in vitro, via coiled-coil domain), but these are much less stable than the heterodimer formed with GMNN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the geminin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014707093.1">
        <property type="nucleotide sequence ID" value="XM_014851607.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070371654.1">
        <property type="nucleotide sequence ID" value="XM_070515553.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070371655.1">
        <property type="nucleotide sequence ID" value="XM_070515554.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070371656.1">
        <property type="nucleotide sequence ID" value="XM_070515555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LGC0"/>
    <dbReference type="SMR" id="A0A8C4LGC0"/>
    <dbReference type="Ensembl" id="ENSEAST00005011793.2">
        <property type="protein sequence ID" value="ENSEASP00005010846.1"/>
        <property type="gene ID" value="ENSEASG00005007649.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837862"/>
    <dbReference type="KEGG" id="eai:106837862"/>
    <dbReference type="CTD" id="51053"/>
    <dbReference type="GeneTree" id="ENSGT00940000153270"/>
    <dbReference type="OMA" id="HWNDQLI"/>
    <dbReference type="OrthoDB" id="10043826at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030030">
        <property type="term" value="P:cell projection organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045786">
        <property type="term" value="P:negative regulation of cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008156">
        <property type="term" value="P:negative regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22589">
        <property type="entry name" value="geminin_CC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1180:FF:000001">
        <property type="entry name" value="Truncated geminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1180">
        <property type="entry name" value="Geminin coiled-coil domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022786">
        <property type="entry name" value="Geminin/Multicilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13372">
        <property type="entry name" value="GEMININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13372:SF4">
        <property type="entry name" value="GEMININ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07412">
        <property type="entry name" value="Geminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111469">
        <property type="entry name" value="Geminin coiled-coil domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="6 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="49"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="161"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="102"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="172"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="Ensembl" id="ENSEASP00005010846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="211" mass="23858" checksum="F066A3FF0C83316E" modified="2022-01-19" version="1">MMNPSMKQKQEGIQENVKNSPIPRRTLKMIQPSAAGSLVGRENELVKVLSKRKHRKDQLTSKTSSSGVVIVPEHSENKSPGGVTQEAFDLMITENPSSQYWKEVAEKRRKALYEALKENEKLHKEIEQKDNEIARLKKENRELAEVAEHVQYMAEVIERLNGEPLDNFESPDSQEFDSEEETGEDSEVEDSEIATCAEEIVSSSTDAKPCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LGC4</accession>
    <name>A0A8C4LGC4_EQUAS</name>
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        <recommendedName>
            <fullName evidence="10">Immunoglobulin superfamily member 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">IGSF3</name>
    </gene>
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        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
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    <dbReference type="AlphaFoldDB" id="A0A8C4LGC4"/>
    <dbReference type="Ensembl" id="ENSEAST00005009636.2">
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        <property type="gene ID" value="ENSEASG00005006358.2"/>
    </dbReference>
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    <dbReference type="GeneTree" id="ENSGT00940000155177"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin superfamily member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000604">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulin superfamily member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000689">
        <property type="entry name" value="Immunoglobulin superfamily member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000827">
        <property type="entry name" value="Immunoglobulin superfamily member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000932">
        <property type="entry name" value="Immunoglobulin superfamily member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin superfamily, member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051102">
        <property type="entry name" value="IgSF_V-set/TM_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207:SF21">
        <property type="entry name" value="IMMUNOGLOBULIN SUPERFAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 13" id="KW-0732">Signal</keyword>
    <keyword evidence="2 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040494442" description="Immunoglobulin superfamily member 3" evidence="13">
        <location>
            <begin position="20"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="1124"/>
            <end position="1147"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="6"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="143"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="276"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="401"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="559"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="676"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="813"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="14">
        <location>
            <begin position="949"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="997"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1007"/>
            <end position="1027"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00067302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008847.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1194" mass="134874" checksum="DCE8E42BE7CD6DDB" modified="2023-09-13" version="2">MKCFFPVLSCLAVLGVVSAQRQVAVQEGPLYRTEGSHITIWCNVSGYQEPSEQNFQWSIYLPSAPEREVQIVSTMDSSFPYAIYTQRVRSGKIYVERVQGNSALLHITDLQARDTGEYECHTPNTEKQYFGSYSAKMNLVVIPDSLQTTAVPQTLHKVEQDPLELTCEVATETFQHSHLSVTWLRQRGGEKPVEVISLSRDFVLHSSSEYGQRQSLGEVRLDKLGSTTFRLTVFHLQPSDQGEFYCEAAEWIQDPDGSWYAITRKRSEGTVVNVQPTDKEFTVRLETEKRLYTVGEPVEFRCILEAQNIPDRYFAVSWAFNSSLIASMGPNAVPVLNSEFTHREARGQLKVAKESDSVFVLKIYHLRQEDGGKYNCRVTEREKTVTGEFIDKESKRPKNIPIIVLPIKSSISVEVASNASVILEGENLHFSCSVRTAGRPQGRFSVIWQLVDRQNRRSNIMWLDRDGTVQPGASYWERSSFGGVQMEQVQPNSFSLGIFNSRREDEGQYECHVTEWVRAVDGEWQIVGERRASTPVSITALETGFAVTAISRTPGVTYSDSFDLQCIIKPHYPARVPVSVTWRFQPVGTVEFHDLVTFTRDGGVQWGDRSSSFRTRTAIEKAESSNNVRLSISRASDTEAGKYQCVAELWRRNYNNTWTRLAERTSNLLEIRVLQPVTKLQVSKSKRTLTLVEDRPIQLNCSVKSQTSQNSHFAVLWYVHKPSDADGKLILKTTHNSAFEYGTYAEEEGLRARLQFERHVSGGLFSLTVQRAEVSDSGSYYCHVEEWLLSPNYAWYKLAEEVSGRTEVTVKQPDSRLKLSQAQGNLSVLETQQVQLECVVVNRTRVASQLVVEWFVWKPNHPERETVARLSREATFHYGEQAAKNNLEGRLHLESPSSGVYRLFIQNVAVQDSGTYSCRVEEWLPSPSGVWYKRAEDTAGQTAVTVMRPDAALQVDTVVPNATVSEKAAFQLDCSIVSRSSQDSRFAVAWYSLRTKAGGKRGSPGLEEQEEEGEEGAEAEEGEEEDPMERMALLSVGPDAVFGPEGSPWEGRLRFQRLSPLLYRLTVLQASPRDTGNYSCRVEEWLPSPQKEWYRLTEEESAPIGIRVLDTSSTLQSVICSNDALFYFIFFYPFPIFGILIITILLVRFKSRNSSKNSDGKNGVPLLWIKEPHLNYSPTCLEPPVLSIHPGAID</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LGZ3</accession>
    <name>A0A8C4LGZ3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L-2-hydroxyglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="7">1.1.99.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">L2HGDH</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(S)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2</text>
            <dbReference type="Rhea" id="RHEA:21252"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:16782"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="EC" id="1.1.99.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the L2HGDH family.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.2" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4LGZ3"/>
    <dbReference type="Ensembl" id="ENSEAST00005012836.2">
        <property type="protein sequence ID" value="ENSEASP00005011814.2"/>
        <property type="gene ID" value="ENSEASG00005008274.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00490000043421"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047545">
        <property type="term" value="F:(S)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008726">
        <property type="entry name" value="PRK11728.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104:SF2">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="9">
        <location>
            <begin position="50"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011814.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="463" mass="50669" checksum="BA1972E87D6ADA59" modified="2023-09-13" version="2">MAPALRYLGGACARARGGFSGALAWGWGPAPGAPWPLGRGGRRASTSSFDVVIVGGGIVGLASARALILRHPALSIGVLEKEKDLAVHQTGHNSGVIHSGIYYKPESLKARLCVQGAALIYEYCNQKGISYKQCGKLIVAVEQEEIPRLQALYERGLQNGVRGLRLIQPEDIKKKEPYCRGLMAIDCPYTGIVDYRQVALSFAQDFQEAGGSVLTNFEVKEIETAKESPSRSKDGVTYPIVIRNTKGEEVRCQYVVTCAGLYSDRISELSGCNPDPRIVPFRGDYLVLKPEKRYLVKGNIYPVPDSRFPFLGVHFTPRMDGSIWLGPNAVLALKREGYRPFDFSARDVMDVIIKSGLIKLVFQNFSYGVNEMYKALFLSATVKHLQKFIPEITVSDIRRGPAGVRAQALDRAGNLVEDFVFDGGVGDMGNRILHVRNAPSPAATSSLAISGMIADEVQQRFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LJW1</accession>
    <name>A0A8C4LJW1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Dehydrodolichyl diphosphate synthase complex subunit NUS1</fullName>
            <ecNumber evidence="6">2.5.1.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Dehydrodolichyl diphosphate synthase complex subunit nus1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">Nogo-B receptor</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="24">Nuclear undecaprenyl pyrophosphate synthase 1 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">NUS1</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">With DHDDS, forms the dehydrodolichyl diphosphate synthase (DDS) complex, an essential component of the dolichol monophosphate (Dol-P) biosynthetic machinery. Both subunits contribute to enzymatic activity, i.e. condensation of multiple copies of isopentenyl pyrophosphate (IPP) to farnesyl pyrophosphate (FPP) to produce dehydrodolichyl diphosphate (Dedol-PP), a precursor of dolichol phosphate which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER). Synthesizes long-chain polyprenols, mostly of C95 and C100 chain length. Regulates the glycosylation and stability of nascent NPC2, thereby promoting trafficking of LDL-derived cholesterol. Acts as a specific receptor for the N-terminus of Nogo-B, a neural and cardiovascular regulator.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>n isopentenyl diphosphate + (2E,6E)-farnesyl diphosphate = a di-trans,poly-cis-polyprenyl diphosphate + n diphosphate</text>
            <dbReference type="Rhea" id="RHEA:53008"/>
            <dbReference type="Rhea" id="RHEA-COMP:19494"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:136960"/>
            <dbReference type="ChEBI" id="CHEBI:175763"/>
            <dbReference type="EC" id="2.5.1.87"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">The active dehydrodolichyl diphosphate synthase complex is a heterotetramer composed of a dimer of heterodimer of DHDDS and NUS1. Interacts with NPC2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the UPP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.87" evidence="6"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4LJW1"/>
    <dbReference type="Ensembl" id="ENSEAST00005014240.2">
        <property type="protein sequence ID" value="ENSEASP00005013105.2"/>
        <property type="gene ID" value="ENSEASG00005009141.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003223"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904423">
        <property type="term" value="C:dehydrodolichyl diphosphate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042632">
        <property type="term" value="P:cholesterol homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006489">
        <property type="term" value="P:dolichyl diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903589">
        <property type="term" value="P:positive regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090050">
        <property type="term" value="P:positive regulation of cell migration involved in sprouting angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032383">
        <property type="term" value="P:regulation of intracellular cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038084">
        <property type="term" value="P:vascular endothelial growth factor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1180.10:FF:000008">
        <property type="entry name" value="NUS1, dehydrodolichyl diphosphate synthase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038887">
        <property type="entry name" value="Nus1/NgBR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21528">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT NUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21528:SF0">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT NUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="7" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="11" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="26">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00058032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00065178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00068682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00074069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00075582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00079334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005013105.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="293" mass="33162" checksum="D50E2978FC9D92F0" modified="2023-09-13" version="2">MTGLYELVWRVLHALLCLHRTLTSWLRVRFGTWNWIWRRCCRAASAAVLAPLGFTLRKRPAAGRNRRHHRHPRGGPGPAGAHLRLRWRADGRALEKLPVHMGLVITEEEQEPSFSDIASLVVWCMAVGISYISVYDHQGIFKRNNSRLMDEILKQQQELLGLDCSKYSPEFANSNDKDDQVLNCQSAVKVLSPEDGKADIVRAAQDFCQLVAQQQKKSTDLDVAMLDSLLSSNGFPDPDLVLKFGPVDSTLGFLPWHIRLTEIVSLPSHLNISYEDFFSALRQYAACEQRLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LPA1</accession>
    <name>A0A8C4LPA1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="26">Transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="30" type="primary">SLC6A4</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>serotonin(out) + K(+)(in) + Na(+)(out) + H(+)(in) = serotonin(in) + K(+)(out) + Na(+)(in) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:75839"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:350546"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:75840"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Neuron projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Endosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="22">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A4 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014682535.2">
        <property type="nucleotide sequence ID" value="XM_014827049.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070338734.1">
        <property type="nucleotide sequence ID" value="XM_070482633.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070338735.1">
        <property type="nucleotide sequence ID" value="XM_070482634.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070338736.1">
        <property type="nucleotide sequence ID" value="XM_070482635.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LPA1"/>
    <dbReference type="Ensembl" id="ENSEAST00005015091.2">
        <property type="protein sequence ID" value="ENSEASP00005013900.1"/>
        <property type="gene ID" value="ENSEASG00005009690.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822048"/>
    <dbReference type="KEGG" id="eai:106822048"/>
    <dbReference type="CTD" id="6532"/>
    <dbReference type="GeneTree" id="ENSGT00940000157855"/>
    <dbReference type="OMA" id="GEDCQGN"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005925">
        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045121">
        <property type="term" value="C:membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043005">
        <property type="term" value="C:neuron projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005178">
        <property type="term" value="F:integrin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005326">
        <property type="term" value="F:neurotransmitter transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050998">
        <property type="term" value="F:nitric-oxide synthase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051378">
        <property type="term" value="F:serotonin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:serotonin:sodium:chloride symporter activity"/>
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    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
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    <sequence length="535" mass="59772" checksum="F593F0B21B53AA38" modified="2023-09-13" version="2">MYHRRTSVKGPSAPTQIYQQPPRLLIVHIALPSWADICSNLCEALQNFFSLACSLMGPSRMSLFSLYMVRNQHECVLPFVQVKGNFARLQACISELRMLQREGCFRPQGASLQMAVEDGLQQFKQYSRHVTTGAALTYTSLEITVLTSQPGKEVVKQLEEGLKDTDLVRVRRLQVVEITKGVLEGMDSASPVEEPSNDESSILGTDIDLQTIDNDIVSMEIFFKAWLHNSGTDQEHMHLLLPSRSYSSTSRARDNPMCLKCDLQERLLSPSLLPGTADGSLRMDDPKGDFSTLYQMASQSSASPYKLRVIKALKSSGVCESLTYGLPFILRPTSCWQLDWDELETNQQHFHALCHSLLKREWLLLAKGEPLSGGHSMREPASTFYVILPSRSPTLLVKAVATRELMLPSPFPLLPEDLPDDSLKIVESTLDSLELEPTYNPLQVWSHLYSHLSSASVKPQGRLHPSWESRALRKHPCKAGQLQMNRVRATVAPLTMTPAPGKAHKMLAASKASSEAFFQLSMEEEAEEEEYLSGP</sequence>
</entry>
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    <accession>A0A8C4LS12</accession>
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        <recommendedName>
            <fullName evidence="8">MHC class I-like antigen recognition-like domain-containing protein</fullName>
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        <name type="scientific">Equus asinus</name>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="X."/>
                <person name="Zhao"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
                <person name="Y."/>
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                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Ju"/>
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                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
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                <person name="J."/>
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                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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            <location evidence="1">Membrane</location>
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    <dbReference type="AlphaFoldDB" id="A0A8C4LS12"/>
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        <property type="gene ID" value="ENSEASG00005031406.1"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="UL16 BINDING PROTEIN 21"/>
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    <keyword evidence="4" id="KW-1015">Disulfide bond</keyword>
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    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="255"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="MHC class I-like antigen recognition-like" evidence="8">
        <location>
            <begin position="63"/>
            <end position="229"/>
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    </feature>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="1"/>
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        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
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        <source>
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        </source>
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    </evidence>
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</entry>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="Liu"/>
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                <person name="Amills"/>
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                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="12" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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            <location evidence="1">Cell membrane</location>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="T cell receptor alpha variable 38-1"/>
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    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
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    <dbReference type="PANTHER" id="PTHR19367:SF47">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF07686">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulin"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
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    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0391">Immunity</keyword>
    <keyword evidence="9" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
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    <keyword evidence="10" id="KW-1279">T cell receptor</keyword>
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        <location>
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            <dbReference type="ARBA" id="ARBA00004236"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
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        </source>
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        </source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="99" mass="11521" checksum="FE5C163022025E24" modified="2023-09-13" version="2">MAQTVTQPQPEISVHEAETVTLNCMYDTSDSDYYLFWYKQPPNGEMILIIRQEAYKQQNATNNRFSVNFQKADKSFGLRISDSQLEDAAMYFCAFMRAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LX58</accession>
    <name>A0A8C4LX58_EQUAS</name>
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        <recommendedName>
            <fullName evidence="13">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="14" type="primary">PHF8</name>
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    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Interacts with POLR1B, UBTF, SETD1A, HCFC1, E2F1 and ZNF711. Interacts with ZNF263; recruited to the SIX3 promoter along with other proteins involved in chromatin modification and transcriptional corepression where it contributes to transcriptional repression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="ENSEASG00005010838.2"/>
    </dbReference>
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        <property type="component" value="Chromosome X"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Histone lysine demethylase PHF8"/>
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    </dbReference>
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        <property type="entry name" value="Cupin"/>
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        <property type="entry name" value="JHDM1_Histone_Demethylase"/>
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    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
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    <dbReference type="PANTHER" id="PTHR23123">
        <property type="entry name" value="PHD/F-BOX CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="JmjC"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="185"/>
            <end position="341"/>
        </location>
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                <person name="Wang"/>
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                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Guo"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
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                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
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                <person name="Yue"/>
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                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
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        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 35"/>
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            <begin position="441"/>
            <end position="1011"/>
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    <sequence length="1103" mass="125034" checksum="B67134F19E83AF2E" modified="2023-09-13" version="2">MDKILEAVVTSSYPASVKQGLVRRVLEAARQPLEREQCLALLALGARLYVTGADELPRRVGCQLLHVAGRHHPDVFAEFFSARRVLRLLQGGAGPPGVRALACVQLGLQLMPEGPAADEVLAVLRRELLRTVRERPGPAACAQVARLLARHPRCVPEGPHRLLFCQQLVRCLGRFRCPAEGEEGAVEFLEQAQQVSGLLAQLWRAQPAAILPCLKELFAVISCTEEEPPSSALASVVQHLPLELMDGVVRNLSNDDSVSDSQMLTAISRMIDWVSWPLGKNIDKWIIALLKGLAAVKKFSILIEVSLAKIEKVFSKLLYPIVRGAALSVLKYMLLTFQHSHEAFHLLLSHIPPMVASLVKEDSNSGTSCLEQLAELVHCMVFRFPGFPDLYEPVMEAIKDLHVPNEDRIKQLLGQDAWTSQKSELAGFYPRLMAKSDTGKIGLINLGNTCYVNSILQALFMASDFRHCVLRLTENNSQPLMTKLQWLFAFLEHSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEKTGTRIYQKLKQSSLPSPPEEPPSPTSTSVEKMFGGKIMTRICCLHCLNVSSREEAFTDLSLAFPPAERCRRRRLGSVMFPAEDIGARDPPSPSRAQVPSRAGPRRQRKHCITGGAPPAVLDIEGLGPQGPWEQRSQEEKVEKEEEAKEERVEEKEERAEEKETVEEKEEEAEEERAEETKERVEKEEEREKEGEQVKEEEERVKEERVKEKEERVKEERVKEKEERVKEERVKEKEERVKEERGERRRRREQRRRRREQRRRRREKRRRQRREEKEKEAENEKKKAEDSLGPGTNRAAATSPGEGSRSVLDLVNYFLSPERLTAENRYYCESCASLQDAEKVVELSQGPRYLILTLLRFSFDLRTMRRRKILDDVSIPLLLRLPLAGGHGQAYDLCSVVVHSGVSSESGHYYCYAREGAARPAPSVGTAERPEPENQWYLFNDTRVSFSSFESVSNVTSFFPKDTAYVLFYRQRPGEGPEAEPGSPRVRAEPTLHKDLMEAISKDNILYLQEQEKEARSRAAYISTLPSSPRWGRGFDEDKDEDEGSPGGCNPAGGNGGDFHRLVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M2P8</accession>
    <name>A0A8C4M2P8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cilia- and flagella-associated protein 44</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">CFAP44</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Flagellar protein involved in sperm flagellum axoneme organization and function.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the CFAP44 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_044771585.2"/>
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        <property type="protein sequence ID" value="ENSEASP00005016527.2"/>
        <property type="gene ID" value="ENSEASG00005011452.2"/>
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    <dbReference type="CTD" id="55779"/>
    <dbReference type="GeneTree" id="ENSGT00940000161555"/>
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        <property type="component" value="Chromosome 5"/>
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    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:cell morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0006997">
        <property type="term" value="P:nucleus organization"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0007288">
        <property type="term" value="P:sperm axoneme assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="Cilia- and flagella-associated protein 44"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cilia- and flagella-associated protein 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055439">
        <property type="entry name" value="Beta-prop_EML_1st"/>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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        <property type="entry name" value="WD40_repeat_CS"/>
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    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
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        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 43-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14885:SF3">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23409">
        <property type="entry name" value="Beta-prop_EML"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
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    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="7 15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 14" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="EML-like first beta-propeller" evidence="17">
        <location>
            <begin position="193"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="14">
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            <begin position="366"/>
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    </feature>
    <feature type="repeat" description="WD" evidence="14">
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            <begin position="469"/>
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    <feature type="repeat" description="WD" evidence="14">
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            <begin position="574"/>
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            <begin position="1499"/>
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        <location>
            <begin position="1416"/>
            <end position="1468"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="1560"/>
            <end position="1615"/>
        </location>
    </feature>
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        <location>
            <begin position="1650"/>
            <end position="1705"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="1771"/>
            <end position="1834"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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            <begin position="30"/>
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            <begin position="46"/>
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    </feature>
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            <begin position="700"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
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            <begin position="1062"/>
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    </feature>
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        <location>
            <begin position="1312"/>
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    </feature>
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        <location>
            <begin position="1504"/>
            <end position="1537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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            <dbReference type="ARBA" id="ARBA00022574"/>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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            <dbReference type="ARBA" id="ARBA00023273"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00055223"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00060934"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00074727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF23409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005016527.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1855" mass="213156" checksum="10F2D21B898CA02F" modified="2023-09-13" version="2">MKEPDDQDTDGGKSDRSKSDRRPSQGSSKSEPRSAVKEDYKFLENTTDETVTEGEESYLEDDDSYTEHLEGSSSSLQEDYSESVGESRYMETPAPAAEEAEEKVKKKISETFFYDYTQLISTPFVTPDSNIPLDLLTLVHSFGYDCRKRANLQLLDNNTVLYVAGNQLILLNLKTKEQIYLRSSSGTGIGVIGVHPDKTYFTVAEKGFSPKIVVYEYPSLRPYRILRDGTDLGYAYVDFNYNGTLLASVGGNPDHTLTIWNWKEEQPILRTKAFSQEVFKVTFNPEDDEQLTTSGSGHIKFWEMALTFTGLKLQGLLGRFGKTATTDIEGYMELPDGKVLSGSEWGNMLVWEAGLIKVELCRAARKSCHQGPINQIVLDEGEVITVGSDGCIRIWDFETIDTADSIDDTGLLEIEPINELQVDKNVKLFSMIKMNETGHNFWLAQDASGAIWKLDLSFSNITQDPECLFSFHSGAIEALALSPLTYLMATTALDCSVRIYDFASKTPLAQMRFKQGGTALAWVPLLVSYTGAQIIAGFEDGVVRILELYDPKKLTVFAGRKKILDAEIRLKQVFKPHTAHVTALAYERDGEILATGSKDQTVFFFEVEKGYKPIGYITTPGPVCQLLWSPSSHPESTLLIICENGYVLEAPLPTIEEEEEGHDVVSYEIKDMSIKCFHFSSVKSKILRLIELEKRKKKKELREKEKEERRKQLAAEMGEDGEKELQGKEEEKEEEEEEPLPQIFIPSTPCHILCGFYSGPGKFWVSLGGYDAGFLYHCAFPPYDKSRDIKEQRDEPFDFRFLQDTEDNPIQAITFSINQDVMFCGMKNGAIRVYILSQNDLSLTNMADYWHFNMHDNNYGCVKGISASFDDRFLVTTGADSNIFVFNVFSEFELKKTIKAKVPSPRFGIETEPLPKDIEDPKTYSIENARRKREHDKLMKEVEEIKAGKREQLKALRTEFWKLLEMNEELPKHMQFKRTDFDVDSKIRAEIDRKTVYKIQQVEKELGWEKEKHRIGLMKLQNRFRDSLESDMVVVHAIQSDHKVSSYRLVKPSKYSRSKRASQSERRTSKFERFEKEGAGRKDSQRDTGGSISVPEESIIEKGKKFRPKTLSEIMVENQIEKTRKLIVKAERAQIKIRQRKKEWEELYKSKPDDDYEDPKDVQAIKEAQLCMGDFNLKTASDYKIPEHMRINAAKKEEELGHLDSLAHSKKMHMNKCILSLRDLKVAVIEEIQCLVQEVKNIQSTLHISKHVPIPQIPQIHPEEVPEKRFQYDEETLLVFKRQQMKSRAGKDPEVECAGSSGSAGGFLRISSGKDGDLTTRDSLSRSSKASSFGLEVPKLLEFERADPTEVELEVMKRDEIKHMYMQQYLTNRIKELIVTFDAELRLLRHQKLKLDTQMKLSDLHHVTLFQEILLLKNFEKQENILQERVNSLDKEEQDLQWKINETLKEMEEKKNEVTRLQDQEKALYAGFQAALGENNKFANFLMKVLKKRIKRSKKKEVEGDADEDEDSDESSEEESSLESDEDESGSEDEVFDDSICPTNCDTGLFELALQLREKRLDIEEALVEEKKTIDNLKKEYDTMSKKVKIVGTNLNAAEEALEAYQREKQQRLNELLVVIPLKLHQIEYVVFGEIPTDLSGTLVFSNHSLGRLQERIVQLQEENSKQQKLNKEYRERRKQLIREKREMAKNINQMEETVRQLMISKFGRVINLEALQTLSVNTTLEELKIKKLRKELSNAKEMKMWEEKIAQMRWELMMKTKEHTKKLHQMNDLCIEKKKLDSRLNTLQNQQGNAFQGPRKADIVAREKVTELIQVQAERISALKKEIALLRKKGGFILPPIQPPQENQMKPMDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M398</accession>
    <name>A0A8C4M398_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TBCC domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">TBCCD1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a role in the regulation of centrosome and Golgi apparatus positioning, with consequences on cell shape and cell migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TBCC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014698827.1">
        <property type="nucleotide sequence ID" value="XM_014843341.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044627191.1">
        <property type="nucleotide sequence ID" value="XM_044771256.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070365363.1">
        <property type="nucleotide sequence ID" value="XM_070509262.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070365364.1">
        <property type="nucleotide sequence ID" value="XM_070509263.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070365365.1">
        <property type="nucleotide sequence ID" value="XM_070509264.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M398"/>
    <dbReference type="Ensembl" id="ENSEAST00005022181.2">
        <property type="protein sequence ID" value="ENSEASP00005020432.2"/>
        <property type="gene ID" value="ENSEASG00005014056.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106832531"/>
    <dbReference type="KEGG" id="eai:106832531"/>
    <dbReference type="CTD" id="55171"/>
    <dbReference type="GeneTree" id="ENSGT00470000042284"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031616">
        <property type="term" value="C:spindle pole centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051661">
        <property type="term" value="P:maintenance of centrosome location"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051684">
        <property type="term" value="P:maintenance of Golgi location"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.70:FF:000005">
        <property type="entry name" value="TBCC domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017901">
        <property type="entry name" value="C-CAP_CF_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016098">
        <property type="entry name" value="CAP/MinC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036223">
        <property type="entry name" value="CAP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006599">
        <property type="entry name" value="CARP_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039589">
        <property type="entry name" value="TBCC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012945">
        <property type="entry name" value="Tubulin-bd_cofactor_C_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16052">
        <property type="entry name" value="TBCC DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16052:SF0">
        <property type="entry name" value="TBCC DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07986">
        <property type="entry name" value="TBCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00673">
        <property type="entry name" value="CARP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69340">
        <property type="entry name" value="C-terminal domain of adenylylcyclase associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51329">
        <property type="entry name" value="C_CAP_COFACTOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C-CAP/cofactor C-like" evidence="8">
        <location>
            <begin position="290"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020432.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="557" mass="63361" checksum="475174BC5AED83E3" modified="2023-09-13" version="2">MDQSGVLLWVKAEPFMVGALQVPPPSKFSLHYLRKISTYVRTRATEGAYPRLYWSTWRHIACGKLQLAKDLAWLYFEIFDSLAVKTPEERLEWSEILSNCMTEDEVEKQRNQLSVDTLQFLLFLYIQQLNKVSLRTSLIGEEWPSPRNRSQSPDLTEKSSCHNKNWNDYSHQAFVYDHLSDLLELLLDPEQLTASFHSTHSSLVSQEAVVALSFLIEGTASRAGKIYPLHELALWQPLHAENGFSKISKTFSFYKLEAWLRACLTGNPFGTSACLKSGKKLAWAHQVEGTTKRAKIACNTHVAPRMHRMVVMSQVYKQTLAKSSDTLVGAHVKIHRCNESFIYLLSPLRSVTIEKCRNSTFVLGPVQTALHLHSCDNIKVIAVCHRLSISSTTGCVFHILTPTRPLILSGNQTVTFAPFHTHYPMLEDHMARTGLATVPNYWDKPMVVCRENSDTSVFRLLPPCEFYIFIIPFEMEGDTTEIPGGLPSAYQKALGQREQKIQIWQKIVKEARLTKDQRKQFQVLVENKFYEWLIHTGHRQQLDSLVPPAAGSKQAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M3H2</accession>
    <name>A0A8C4M3H2_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Kelch like family member 35</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">KLHL35</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M3H2"/>
    <dbReference type="Ensembl" id="ENSEAST00005022494.2">
        <property type="protein sequence ID" value="ENSEASP00005020721.2"/>
        <property type="gene ID" value="ENSEASG00005014246.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161093"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd18265">
        <property type="entry name" value="BTB_POZ_KLHL35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000001">
        <property type="entry name" value="Kelch-like family member 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030601">
        <property type="entry name" value="KLHL35_BTB_POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412">
        <property type="entry name" value="KELCH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24412:SF187">
        <property type="entry name" value="KELCH-LIKE PROTEIN 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="41"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020721.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="587" mass="63003" checksum="B6F0E63115AFEA03" modified="2023-09-13" version="2">MREGPGPEESEPGSEAPCAGSCHAQRVLQTLNAYRRSGTLTDVVLRAGGRDFPCHRAALSAGSAYFRSLFAAGRPERGPALGPAVPVVQVAPDAPGAGPAGAAAAALAVVLDYVYGAGVRLRAEDEAAAVLALAERLGVAGLREACARFLEGRLRAANSLALRRVAAAFSLTSLAERCGRVLRQAFAEVARHADFLELGPDEVAALLADPALGVAREEAVFEAAMRWVRHDAPGRRGQLRRLLEHVRLPLLAPAYFLEKVEADELLQACGECRPLLLEARACFILGREAGALRARPRRFMDLAEVIVVIGGCDRKGLLKLPFADAYHPESRRWTPLPSLPGYTRSEFAACALRNDVYVSGGHINSRDVWMFSSHLHTWIKVASLHKGRWRHKMAVLQGQLFAVGGFDGLRRLRSVERYDPFSNTWAAAAPLPEAVSSAAVAPCAGQLYVIGGAGQDGVSTDKVQCFDPKEDRWSLRAPAPFSQRCLEAVSLEDTIYVVGGLMSRIFTYDPSTDVWGEAAVLPSPVESCGVTVCDGKVHVVGGRDDHGESTDRVFTFDPSSGQVEAQPSLQRCTSSHGCVTIVQSLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M5C7</accession>
    <name>A0A8C4M5C7_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Activating transcription factor 7 interacting protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">ATF7IP</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MCAF family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4M5C7"/>
    <dbReference type="Ensembl" id="ENSEAST00005023203.2">
        <property type="protein sequence ID" value="ENSEASP00005021376.2"/>
        <property type="gene ID" value="ENSEASG00005014635.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063707"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005667">
        <property type="term" value="C:transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006346">
        <property type="term" value="P:DNA methylation-dependent constitutive heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026085">
        <property type="entry name" value="ATF7-int"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031870">
        <property type="entry name" value="ATF7IP_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056565">
        <property type="entry name" value="Fn3_ATF7IP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23210">
        <property type="entry name" value="ACTIVATING TRANSCRIPTION FACTOR 7 INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23210:SF22">
        <property type="entry name" value="ACTIVATING TRANSCRIPTION FACTOR 7-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16788">
        <property type="entry name" value="ATF7IP_BD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16794">
        <property type="entry name" value="fn3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fibronectin type-III" evidence="9">
        <location>
            <begin position="1153"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="108"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="448"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="651"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="815"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="879"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1108"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="193"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="267"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="300"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="339"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="470"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="490"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="517"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="670"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="815"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="836"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="910"/>
            <end position="922"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="923"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="981"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1009"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1121"/>
            <end position="1144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005021376.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1263" mass="136072" checksum="046C948B6AA0D78F" modified="2023-09-13" version="2">MHQDQRFKMDGIEEPQKKVFKARKTMRVSDRQQLEAVYKVREELLKTDVKLLNGNHENGDLDPTSPLENMDYINDKEEVNGIEELCFDPEKSKSEWKETPCTLNINVKNKQDDDLKCESSSPNNVAPELASKLTAEPTSDPATGDLATEDLVSGDSASGDVASEVLTSGDPTSGDPASHNFTCSDPVSGDPASGEPVSGEPVSGESVSGEPVSSELVSGEPVPGELVSSEPVSSDPASDDSSFGDPASGALTSDDPASGDLASGDPASDEPTSDELTPESAFDPTFEPSSVPVCESVPETDNREFSSNKGDDFLEKNRDDDKLDQIFSFDEKNKVDSNIDADEETLETDDTIICSDLPPENEKKVEEDAIIEPALGEEAISSSIEIDQSEKNEDENSADLAETINENVMKDDKNENILENTDSMETDEIIPILEKLAPAEDELTCFSKTSILPIDETNPDLEEKMEGSFGSPSKQESSESLPKEAFLVLSDEEDISGEKDESEVISQNETCSAAEEESNKKDSKPEEEEQVIHEDERPSEKSEFSRRKRSKSEDMDNVQSKRRRYMEEEYEAEFQVKITAKGDINQKLQKVVQWLLEEKLCALQCAVFDKTLAELKTRVEKIECNKRHKTVLTELQAKIARLTKRFGAAKEDLKKRQENPPNPPVSPGKSVVDVNSNNSVPYRNVTTVRQMLESKRNVSESAPPSFQTPVNTVSSTNLVTPPAVVTSQPKLQTPVTSSSLTAPSVLPAPNTATVVATTQVPSGNPQPTISLQPLPVILHVPVAVSSQPQLLQSHPGTLVTNQPSGNVEFISVQSPPTVSGLTKNPVSLPSLPNPTKPNNVPSVPSPSIQRNSPASAAPLGTTLAVQAVPTAHSIVQATRTSLPTVGPSGLYSPSNNRGPIQMKIPISAFSTSSSAEQTSTTTPRIENQTNKTIDASVNKKAADSTSQSGKAAGSDSGGVIDLTMDDEESGASQDPKKLNHTPVSTMGSSQPVSRPLQPIQPAPPLQPSGVPTSGPSQTTIHLLPTAPTTVNVTHRPVTQVTTRLPVPRAPANHQVVYTTLPAPPAQAPLRGTVMQAPAVRQVNPQNSVTVRVPQATTYVVNNGLTLGSTGPQLTVHHRPPQVHPEPPRPVHPAPLPEAPQPQRLPPEAASTSLPQKPHLKLARVQSQNGIVLSWSVLEVDRSCATVDSYHLYAYHEEPSATVPSQWKKIGEVKALPLPMACTLTQFVSGSKYYFAVRAKDIYGRFGPFCDPQSTDVISSSQSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M6G7</accession>
    <name>A0A8C4M6G7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="4">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">HERC4</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4M6G7"/>
    <dbReference type="Ensembl" id="ENSEAST00005024408.2">
        <property type="protein sequence ID" value="ENSEASP00005022491.2"/>
        <property type="gene ID" value="ENSEASG00005015346.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158924"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000012">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000004">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2410.10:FF:000003">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1750.10:FF:000010">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051709">
        <property type="entry name" value="Ub-ligase/GTPase-reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45622:SF5">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HERC4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45622">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="RCC1" evidence="10">
        <location>
            <begin position="46"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="10">
        <location>
            <begin position="99"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="10">
        <location>
            <begin position="151"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="10">
        <location>
            <begin position="204"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="HECT" evidence="11">
        <location>
            <begin position="620"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="9">
        <location>
            <position position="915"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005022491.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="947" mass="106865" checksum="75560A1AC056F6FD" modified="2023-09-13" version="2">MSIDTLVRTCSGISYGRIRNIKSLSDIQIVQVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDYKKQASPQLIKSLLGIPFMQVAAGGAHSFVLTLSGAIFGWGRNKFGQLGLNDENDRYFPNLLKSLRTQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTSHEINPRKVFELMGSIVTQIACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFPYNGQCPTDIDSEEYFCVKRIFSGGDQSFAHYSNPQNCRPPDDFRCTDPSKQIWTVNEALIQKWLSYPSGRVPVEIANEIDGTFSSSGCLNGSFLAVSNDDHYKTGAKFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNLIPKLTGSLPDVEALRFYLTLPECPLMNDPNNFITIAIPFGRALVNLEKAPLKVLEKWWSVLEPPLFLKIVELFKEVVVHLLKEYKRCIASPERRIFNSFLHTALKVLEILHRVNEKSGQIIQYDKFYIHEVQELIDIRNDYINWVQQQAYGMDVNHGLTELADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPCLILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYEDSRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKKKPSLDDLKELVPDVGRSMQQLLDYPEDDVEETFCLNFTITVENFGATEVKELIVNGADTAVNKQNRKEFVDAYVDYIFNKSVASLFDAFHAGFHKVCGGKVLQLFQPNELQAMVIGNTNYDWKELEQNTEYKGEYWAEHPTIKIFWEVFHELPLEKKKQFLLFLTGSDRIPILGMKSLKLAIQSTGGGEEYLPVSHTCFNLLDLPKYTEKETLRSKLIQAIDHNEGFSLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MB61</accession>
    <name>A0A8C4MB61_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">GPI ethanolamine phosphate transferase 3, catalytic subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Phosphatidylinositol-glycan biosynthesis class O protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PIGO</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGG/PIGN/PIGO family. PIGO subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MB61"/>
    <dbReference type="Ensembl" id="ENSEAST00005021729.2">
        <property type="protein sequence ID" value="ENSEASP00005020009.1"/>
        <property type="gene ID" value="ENSEASG00005013817.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00910000144278"/>
    <dbReference type="OMA" id="YPSFDIF"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051377">
        <property type="term" value="F:mannose-ethanolamine phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd16023">
        <property type="entry name" value="GPI_EPT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000041">
        <property type="entry name" value="GPI ethanolamine phosphate transferase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037675">
        <property type="entry name" value="PIG-O_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039524">
        <property type="entry name" value="PIGO/GPI13"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071:SF1">
        <property type="entry name" value="GPI ETHANOLAMINE PHOSPHATE TRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23071">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="448"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="481"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="508"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="539"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="572"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="668"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="703"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="734"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="831"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="857"/>
            <end position="879"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="944"/>
            <end position="963"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1010"/>
            <end position="1030"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1042"/>
            <end position="1064"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008695"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
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            <dbReference type="ARBA" id="ARBA00022679"/>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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            <dbReference type="ARBA" id="ARBA00022824"/>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00093602"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020009.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="1094" mass="119289" checksum="58A79D1E630DE8C6" modified="2022-01-19" version="1">MQKISVLLFLAWVSFLFYAGIALFTGGFLLTRLELSNHSSCREPPGPGSLPWGSRGEPGACWMASRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSLPFLGKLGSLQRILEIQPHHARLYQSKAHPPTTTMQRLKALTTGSLPTFIDAGSNFASYAIVEDNLIKQLTNAGRRVVFMGDDTWKDLFPGAFSQAFFFPSFNVRDLHTVDNGILEHLYPTMDSGEWDVLIAHFLGVDHCGHKHGPYHPEMAKKLSQMDQVIQELVERLENDTLLVVIGDHGMTMSGDHGGDSELEISAALFLYSPAALFPSAPPEEPEVIPQISLVPTLALLLGLPIPFGNIGEVIAELFSEVEDSQPHFSALAQASALHLNAQQVSRFLYTYSAAAQDLQVKELHRLQNLFSKASADYQRLLQSPQRAEAALQTVIPELQQFLRGVQAMCLESWARFSLIRMAVGAAILAAACFLCLLVSQWATSPGFCFHRLLLIPMAWGLAGAIVCAGLLATTELKLDPVILGAMAAVGSLLPFLWKASADWGSKRPLAALLPIPGPVLLLLLIRFAAFFSDSFVVAEARATPFLLGSLILLLVVRLHWEGRLLPPKLLTIPRLGFSAPTGPPRHNGVQALGLGVGLLLCIRLAGLFHRCPEETPACRSSPWLSPLASMVGGRAKNLWYGACVGALVALLAAVRLWLRRYGDLKSPEPPVLFVRWGLPVMVLGTAAYWALASGADEAPPRLRALVAGASVVLPRAVAGLAASGLMLLLWRPVTVLVKAATGAPKSRTVLAPFSGPPTSQADLDYVVPQIYRHMQEEFRGRLERAKPQRPLTVAARQLGSVYSAAVVTALTLLAFPLLLLHAERISLVFLLLFLQSFLLLHLLAAGIPITTPGPFTVPWQAVSAWALMATQTFYSTGHQPVFPAIHWHAAFVGFPEGHGSSTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCESQGPQKRRQPPGNEAEEEEEPLMEMRLRDVPHHFNAALLQLGLKYLFVLGIQILACALAASVLRRHLMVWKVFAPKFIFEAVGFIVSSVGLFLGIALVMRVDGAVNSWFRQLVLAQQSLLLLCHVEVLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MB83</accession>
    <name>A0A8C4MB83_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Replication factor C subunit 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Activator 1 subunit 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">RFC4</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Subunit of the replication factor C (RFC) complex which acts during elongation of primed DNA templates by DNA polymerases delta and epsilon, and is necessary for ATP-dependent loading of proliferating cell nuclear antigen (PCNA) onto primed DNA. The RFC4 subunit probably functions as a scaffold on which the other complex components can assemble.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Subunit of the RFC complex, an heteropentameric complex consisting of a large subunit RFC1 and four small subunits RFC2, RFC3, RFC4 and RFC5; the RFC complex interacts with PCNA. Forms an heterotetrameric complex with RFC2, RFC3 and RFC5; this complex has ATPase activity but is not stimulated by PCNA. The heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 interacts with RAD17. Interacts with ATAD5. Interacts with CTF18. Interacts with CNTD1; this interaction facilitates crossover formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MB83"/>
    <dbReference type="Ensembl" id="ENSEAST00005022241.2">
        <property type="protein sequence ID" value="ENSEASP00005020482.2"/>
        <property type="gene ID" value="ENSEASG00005014105.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074917"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000032">
        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000010">
        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000237">
        <property type="entry name" value="replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="13">
        <location>
            <begin position="70"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00070186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00080378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020482.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="356" mass="38819" checksum="920E6283EDEEEF27" modified="2023-09-13" version="2">MQAFLKGASISAKPLMPKDRGVAATPGSSGENKKAKPVPWVEKYRPKCVDEVAFQEEVVAVLKKSLEGADLPNLLFYGPPGTGKTSTILAAARELFGPELFRLRVLELNASDERGIQVVREKVKNFAQLTVSGSRSDGKPCPPFKIVILDEADSMTSAAQAALRRTMEKESKTTRFCLICNYISRCSKFRFKPLSDKIQQQRLLDIAGKEHVKISNEGIAYLVKVSEGDLRKAITFLQSATRLTGGKEVTEKVITDIAGVIPAETIDGLLAACQSGSFDKLEAAVKDLIDEGHAATQLVNQLHDVIVENDNLSDKQKSIITEKLAEVDKCLADGADEHLQLMSLCATVMQQLTQNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MBU6</accession>
    <name>A0A8C4MBU6_EQUAS</name>
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        <recommendedName>
            <fullName evidence="8">UPAR/Ly6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LY6G6D</name>
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    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MBU6"/>
    <dbReference type="Ensembl" id="ENSEAST00005025102.2">
        <property type="protein sequence ID" value="ENSEASP00005023132.2"/>
        <property type="gene ID" value="ENSEASG00005015762.2"/>
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    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
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        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
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    <dbReference type="GO" id="GO:0030550">
        <property type="term" value="F:acetylcholine receptor inhibitor activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:acetylcholine receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="TFP_LU_ECD_Ly6G6d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016054">
        <property type="entry name" value="LY6_UPA_recep-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026524">
        <property type="entry name" value="LY6G6d/LY6G6f"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32286:SF9">
        <property type="entry name" value="LYMPHOCYTE ANTIGEN 6 COMPLEX LOCUS PROTEIN G6D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32286">
        <property type="entry name" value="LYMPHOCYTE ANTIGEN 6 COMPLEX LOCUS PROTEIN G6F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00021">
        <property type="entry name" value="UPAR_LY6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="UPAR/Ly6" evidence="8">
        <location>
            <begin position="70"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="186" mass="19861" checksum="76DC95DB35ECDDAD" modified="2023-09-13" version="2">MHIHTRAHTPSSVTRLPERLSSSLYLVPLQSPCLGPTAPSNACLARDLGLREGISNRHLEKDTRHQTGNRMRCYDCGGGPSSSCKETVTTCGEGERCGFLERKPRNKPSGNPSVTVIHHHPACVAAHHCNQVETELVGDVTYTTHRDCCIGDLCNSAVAASSVAPACILAAAATALAWLVPGLWRG</sequence>
</entry>
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    <accession>A0A8C4ME03</accession>
    <name>A0A8C4ME03_EQUAS</name>
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        <submittedName>
            <fullName evidence="4">Transmembrane protein 119</fullName>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="J."/>
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                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_044631663.1">
        <property type="nucleotide sequence ID" value="XM_044775728.2"/>
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    <dbReference type="AlphaFoldDB" id="A0A8C4ME03"/>
    <dbReference type="Ensembl" id="ENSEAST00005022984.2">
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        <property type="gene ID" value="ENSEASG00005037151.1"/>
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        <property type="gene ID" value="ENSEASG00005037151.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005066144.1">
        <property type="protein sequence ID" value="ENSEASP00005044306.1"/>
        <property type="gene ID" value="ENSEASG00005037151.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005070735.1">
        <property type="protein sequence ID" value="ENSEASP00005059007.1"/>
        <property type="gene ID" value="ENSEASG00005037151.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106844824"/>
    <dbReference type="KEGG" id="eai:106844824"/>
    <dbReference type="CTD" id="338773"/>
    <dbReference type="GeneTree" id="ENSGT00390000017134"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030501">
        <property type="term" value="P:positive regulation of bone mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045669">
        <property type="term" value="P:positive regulation of osteoblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033690">
        <property type="term" value="P:positive regulation of osteoblast proliferation"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031453">
        <property type="entry name" value="TMEM119"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28645">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28645:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15724">
        <property type="entry name" value="TMEM119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044680410" evidence="3">
        <location>
            <begin position="25"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
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        <source>
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    <evidence type="ECO:0000313" key="4">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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    </evidence>
    <sequence length="275" mass="27996" checksum="1D7DD8434D8A195F" modified="2023-09-13" version="2">MVSAVAPSLVVSLLLLLRALPAAAHSVTLQAAFLEDSGGSGEAEGSSASSPSLPPPRTPALSPTSLGPQPTPLAGPKPPTNFLDGIVDFFRQYVMLIAVVGSLAFLLMFIVCAALITRQKHKASAYYPSSFPKKKYVDQSDRAGGPRAFSEVPDRAPDGRPEEALDSSQQLQADILAATQNLKSPTRATPGAGDGARMTEGQSEEEQGSQAGDQPAQACGDPAEKPEVPEEPCSAGEAGEGQGEPGAAPSLAQEAGDPAGPLESSCAGGSIPPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MEC2</accession>
    <name>A0A8C4MEC2_EQUAS</name>
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        <name evidence="22" type="primary">SMC3</name>
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            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="17">Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex also plays an important role in spindle pole assembly during mitosis and in chromosomes movement.</text>
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        <subcellularLocation>
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        <subcellularLocation>
            <location evidence="1 18">Nucleus</location>
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    </comment>
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        <text evidence="3">Belongs to the SMC family. SMC3 subfamily.</text>
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            <begin position="962"/>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MEF0</accession>
    <name>A0A8C4MEF0_EQUAS</name>
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            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
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                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
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                <person name="Yang"/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
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                <person name="Pan"/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
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                <person name="Y."/>
                <person name="Yue"/>
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                <person name="Liu"/>
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                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="RefSeq" id="XP_014695472.1">
        <property type="nucleotide sequence ID" value="XM_014839986.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A8C4MHP8"/>
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        <property type="protein sequence ID" value="ENSEASP00005027368.2"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="F:serine-type endopeptidase activity"/>
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        <property type="term" value="P:proteolysis"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Complement factor D"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Serine protease 37"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Peptidase_S1_PA"/>
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    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
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    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
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    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
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    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033116">
        <property type="entry name" value="TRYPSIN_SER"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF81">
        <property type="entry name" value="GRANZYME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
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    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
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    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
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    <dbReference type="PROSITE" id="PS00135">
        <property type="entry name" value="TRYPSIN_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2 8" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040104900" description="Peptidase S1 domain-containing protein" evidence="8">
        <location>
            <begin position="19"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="9">
        <location>
            <begin position="21"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    <evidence type="ECO:0000256" key="7">
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    </evidence>
    <evidence type="ECO:0000256" key="8">
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            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="252" mass="27643" checksum="5B88FA7764C449DD" modified="2023-09-13" version="2">MQPLLLLLAFLLSPGAEAGEIIGGHEARPHSRPYMAFVLSLEKEKGKSCGGVLVRQDFVLTAAHCWGSSIKVTLGAHNIQKQERTQQVISVKEAIPHPHYNSKNFNNDIMLLKLERKAMWTAAVRPLSLPRGTAQVRPGEVCSVAGWGSVTPIGRASDTLQEVELTVQQDQVCEFLWHDYNKTTQLCVGDPKEKKSSYKGDSGGPLVCKNVIQGIVSYGRILGIPPGAFTKVSSFLPWIKKTMKGPARWRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MIQ7</accession>
    <name>A0A8C4MIQ7_EQUAS</name>
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        <recommendedName>
            <fullName evidence="18">Very long-chain specific acyl-CoA dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="17">1.3.8.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="34" type="primary">ACADVL</name>
    </gene>
    <organism evidence="34 35">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="34 35" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="34" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="34" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Very long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Among the different mitochondrial acyl-CoA dehydrogenases, very long-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 12 to 24 carbons long primary chains.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>a very-long-chain 2,3-saturated fatty acyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = a very-long-chain (2E)-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:19181"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:83724"/>
            <dbReference type="ChEBI" id="CHEBI:83728"/>
            <dbReference type="EC" id="1.3.8.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:19182"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>dodecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-dodecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47296"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57330"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:47297"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>eicosanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-eicosenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47236"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57380"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:74691"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:47237"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>octadecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-octadecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47240"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:71412"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:47241"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>oxidized [electron-transfer flavoprotein] + hexadecanoyl-CoA + H(+) = (2E)-hexadecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:61526"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:43449"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>tetracosanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-tetracosenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47232"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:65052"/>
            <dbReference type="ChEBI" id="CHEBI:74693"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:47233"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>tetradecanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-tetradecenoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:47316"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:61405"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:47317"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 28">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; mitochondrial fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Homodimer. Homodimerizes after import into the mitochondrion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 28">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.9" evidence="17"/>
    <dbReference type="RefSeq" id="XP_014716976.1">
        <property type="nucleotide sequence ID" value="XM_014861490.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MIQ7"/>
    <dbReference type="Ensembl" id="ENSEAST00005029688.2">
        <property type="protein sequence ID" value="ENSEASP00005027340.1"/>
        <property type="gene ID" value="ENSEASG00005018265.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106844174"/>
    <dbReference type="CTD" id="37"/>
    <dbReference type="GeneTree" id="ENSGT00940000158535"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000062">
        <property type="term" value="F:fatty-acyl-CoA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017099">
        <property type="term" value="F:very-long-chain fatty acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01161">
        <property type="entry name" value="VLCAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000008">
        <property type="entry name" value="acyl-CoA dehydrogenase family member 9, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000017">
        <property type="entry name" value="very long-chain specific acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.40.110.10:FF:000006">
        <property type="entry name" value="very long-chain specific acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000001">
        <property type="entry name" value="Very long-chain-specific acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049448">
        <property type="entry name" value="ACAD9/ACADV-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
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    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
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    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43884:SF11">
        <property type="entry name" value="VERY LONG-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF21343">
        <property type="entry name" value="ACAD9-ACADV_C"/>
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    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
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        <property type="entry name" value="Acyl-CoA_dh_M"/>
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        <property type="entry name" value="ACYL_COA_DH_2"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9 28" id="KW-0274">FAD</keyword>
    <keyword evidence="10" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="6 28" id="KW-0285">Flavoprotein</keyword>
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    <keyword evidence="15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13 28" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
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    <keyword evidence="8" id="KW-0702">S-nitrosylation</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="32">
        <location>
            <begin position="104"/>
            <end position="209"/>
        </location>
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    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="31">
        <location>
            <begin position="213"/>
            <end position="315"/>
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    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="30">
        <location>
            <begin position="327"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="domain" description="ACAD9/ACADV-like C-terminal" evidence="33">
        <location>
            <begin position="527"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="29">
        <location>
            <begin position="11"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="29">
        <location>
            <begin position="54"/>
            <end position="68"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00005198"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
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            <dbReference type="ARBA" id="ARBA00022799"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00049140"/>
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            <dbReference type="ARBA" id="ARBA00049224"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU362125"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Pfam" id="PF00441"/>
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        <source>
            <dbReference type="Pfam" id="PF02770"/>
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            <dbReference type="Pfam" id="PF21343"/>
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        <source>
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    </evidence>
    <sequence length="655" mass="70069" checksum="BF1A762282EA5273" modified="2022-01-19" version="1">MQAARMATSVGRQLLRLGGGSSRPSALLGQPRPGPAQRPYASGATQAVLDKSDSVSSETSTSGKQAKAKSKSFAAGMFKGQLTTDQVFPYPSVLNEEQNQFLKELVGPVSRFFEEVNDPAKNDMLKQVEETTMQGLKDLGAFGLQVPSELGGVGLCNTQYARLVEIVGMHDLGVGITLGAHQSIGFKGILLFGTKAQKEKYLPKLASGETIAAFCLTEPSSGSDAASIRSSAVPSPCGKYYTLNGSKIWISNGGLADIFTVFAKTPVTDAATGAVKEKITAFVVEKSFGGVTHGPPEEKMGIKASNTAEVYFDGVRVPSDNVLGEVGGGFKVAMHILNNGRFGMAAALAGTMKGIIAKAVDHAANRTQFGEKIHNFGIIQEKLARMAMLQYVTESMAYMVSANMDQGSTDFQIEAAISKIFGSEAAWQVTDECIQIMGGMGFMKEAGVERVLRDLRIFRIFEGTNDILRLFVALQGCMDKGKELSKLGSALKNPFGNAGLLLGEAGKQLKRRAGMGSGLSLSGIVHPDLSRSGELAVQALEQFATVVEAKLIKHKKEIVNEQFLLQRLADSAIDLYAMVVVLSRASRSLSEGHPTAQHEKMLCDSWCIEAAARIRETMAALQSDPQQQELFRNFKSISKALVERGGVVTSNPLGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8C4MKM9_EQUAS</name>
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        <recommendedName>
            <fullName evidence="7">Ig-like domain-containing protein</fullName>
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    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="ENSEASP00005027972.2"/>
        <property type="gene ID" value="ENSEASG00005019010.2"/>
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        <property type="component" value="Chromosome 13"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05716">
        <property type="entry name" value="IgV_pIgR_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CMRF35-like molecule 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF115">
        <property type="entry name" value="IMMUNOGLOBULIN SUBTYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="153"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="7">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005027972.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="193" mass="21526" checksum="E0D87855ACBF5F3F" modified="2023-09-13" version="2">PGSLSLSGPRTVTGTIGGSLSVQCRYDEEYREHKKYWCRNSKWPPWRKKIVETTESEREVSRGRVSIRDHPANLTFTVTLESLTEADTGTYCCGIDTRWSEGVLHDSTFQVEVSVLPAAPTPKIFTSTPGPPTTLPATTRSSTTRSLLSSVRFLLLVFLEVPLLLSMLSAVLWVNRPLRGCGQRRSQPDCENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MPV3</accession>
    <name>A0A8C4MPV3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106823669</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cell membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MPV3"/>
    <dbReference type="Ensembl" id="ENSEAST00005032491.2">
        <property type="protein sequence ID" value="ENSEASP00005029896.2"/>
        <property type="gene ID" value="ENSEASG00005025418.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154619"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15234">
        <property type="entry name" value="7tmA_OR7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000009">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48001">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="187"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="31"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005029896.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="307" mass="34340" checksum="BA39EFFEE936942D" modified="2023-09-13" version="2">LKNMEPRNHDPELQPFIFCLFLSMYLVTILGNLLIILAVISDSHLHKPMYFFLSNLSFSDICLSTTTIPKMLVNIQARNQSITYIGCLTQSCFVLVSVGLENCLLAVMAYDRYVAICHPLKYMVIMNSRFCGLMILLSLFISIVDALLHSLMVLQLTFCTNLEIPLFFCEVVQVIKLACSDTLINNIVIYFATSIFGGIPVCGIIFSYAQIFSSFLRMPSVAGKCKTFSTCGSHLSVVCLFYGTGLGMYINSALTKFSINTAVASVMYTAVPQMLNPFIYSLRNRDMKKALRKLIGKIPFVFQECAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MTX9</accession>
    <name>A0A8C4MTX9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Taste receptor type 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC123288005</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the G-protein coupled receptor T2R family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044631627.1">
        <property type="nucleotide sequence ID" value="XM_044775692.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MTX9"/>
    <dbReference type="Ensembl" id="ENSEAST00005033652.2">
        <property type="protein sequence ID" value="ENSEASP00005030951.1"/>
        <property type="gene ID" value="ENSEASG00005021104.2"/>
    </dbReference>
    <dbReference type="GeneID" id="123288005"/>
    <dbReference type="KEGG" id="eai:123288005"/>
    <dbReference type="GeneTree" id="ENSGT01150000286961"/>
    <dbReference type="OMA" id="KFQQPFR"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033038">
        <property type="term" value="F:bitter taste receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000055">
        <property type="entry name" value="Taste receptor type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007960">
        <property type="entry name" value="TAS2R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11394">
        <property type="entry name" value="TASTE RECEPTOR TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11394:SF57">
        <property type="entry name" value="TASTE RECEPTOR TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05296">
        <property type="entry name" value="TAS2R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="3 12" id="KW-0919">Taste</keyword>
    <keyword evidence="10 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="181"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="234"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="301" mass="34474" checksum="3D05679E24F89C8C" modified="2022-01-19" version="1">MVSPLSAILHVLIMSAEFITGITVNGFLIIINCHELIKSRKLTPMQLLFVCIGTSRFGLQIVLMVQSFFSIFFPLLYAVKIYGPVMIFLWMFFSSVSLWFATCLSVFYCLKIAGFTQSYFLWLKFRISKLMPWLLLGSLLASVSIAALCTEVDYPLHVGDILRNTTLKRTELKIKQISEVLLVNLALIFPLAIFVMCTFVLFISLYKHTRRMQKGPHGFSNASTAAHINALRTVITFFCFFISYFAAFMTNITFSVPYRSQNFFVLKFIMAAYPSGHSVIIILYHSKFQQPFRRLLCLKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MWM3</accession>
    <name>A0A8C4MWM3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sorting and assembly machinery component 50 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SAMM50</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Plays a crucial role in the maintenance of the structure of mitochondrial cristae and the proper assembly of the mitochondrial respiratory chain complexes. Required for the assembly of TOMM40 into the TOM complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAM50/omp85 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014719907.1">
        <property type="nucleotide sequence ID" value="XM_014864421.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MWM3"/>
    <dbReference type="Ensembl" id="ENSEAST00005034877.2">
        <property type="protein sequence ID" value="ENSEASP00005032024.2"/>
        <property type="gene ID" value="ENSEASG00005021837.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106845952"/>
    <dbReference type="KEGG" id="eai:106845952"/>
    <dbReference type="CTD" id="25813"/>
    <dbReference type="GeneTree" id="ENSGT00390000011355"/>
    <dbReference type="OrthoDB" id="1724197at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033108">
        <property type="term" value="P:mitochondrial respiratory chain complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045040">
        <property type="term" value="P:protein insertion into mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.50:FF:000002">
        <property type="entry name" value="sorting and assembly machinery component 50 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.310:FF:000010">
        <property type="entry name" value="sorting and assembly machinery component 50 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.310">
        <property type="entry name" value="membrane protein fhac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.50">
        <property type="entry name" value="membrane protein fhac: a member of the omp85/tpsb transporter family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000184">
        <property type="entry name" value="Bac_surfAg_D15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039910">
        <property type="entry name" value="D15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034746">
        <property type="entry name" value="POTRA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815:SF18">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY COMPONENT 50 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12815">
        <property type="entry name" value="SORTING AND ASSEMBLY MACHINERY SAMM50 PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01103">
        <property type="entry name" value="Omp85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51779">
        <property type="entry name" value="POTRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1134">Transmembrane beta strand</keyword>
    <feature type="domain" description="POTRA" evidence="10">
        <location>
            <begin position="45"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005032024.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="469" mass="51877" checksum="57BC25AD62EE4124" modified="2023-09-13" version="2">MGTVHARSLEPLPASGPDFGALGEEAEFVEVEPEAKQEILENKDVVVQHVHFDGLGRTKDDIIMCEIGDVFKAKNLIEVMRKSHEAREKLLRLGIFRQVDVLIDTCQGDDALPNGLDVTFEVTELRRLTGSYNTMVGNNEGSMVLGLKLPNLLGRAEKVTFQFSYGTKETSYGLSFFKPQPGNFERNFSVNLYKVTGQFPWSSLRETDRGVSAEYSFPIWKTSHTVKWEGVWRELGCLSRTASFAVRKESGHSLKSSLSHAMVIDSRNSSILPRKGALLKVNQELAGYTGGDVSFIKEDFELQLNKQLILESVFSASLWGGMLVPIGDKPSSIADRFYLGGPTSVRGFSMHSVGPQSEGDYLGGEAYWAGGLHLYTPLPFRPGQGGFGELFRTHFFLNAGNLCNLNYGEGPKAHIRKLAECIRWSYGAGIVLRLGNIARLELNYCVPMGVQRGDRICDGVQFGAGIRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MXT1</accession>
    <name>A0A8C4MXT1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Beta-defensin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">DEFB105B</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Has antibacterial activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the beta-defensin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MXT1"/>
    <dbReference type="SMR" id="A0A8C4MXT1"/>
    <dbReference type="Ensembl" id="ENSEAST00005035624.2">
        <property type="protein sequence ID" value="ENSEASP00005032695.1"/>
        <property type="gene ID" value="ENSEASG00005022324.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106823822"/>
    <dbReference type="KEGG" id="eai:106823822"/>
    <dbReference type="GeneTree" id="ENSGT00390000002317"/>
    <dbReference type="OMA" id="CRRMCLE"/>
    <dbReference type="OrthoDB" id="9511204at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042742">
        <property type="term" value="P:defense response to bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="InterPro" id="IPR025933">
        <property type="entry name" value="Beta_defensin_dom"/>
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    <dbReference type="Pfam" id="PF13841">
        <property type="entry name" value="Defensin_beta_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0044">Antibiotic</keyword>
    <keyword evidence="6" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="6" id="KW-0211">Defensin</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034402139" description="Beta-defensin" evidence="6">
        <location>
            <begin position="28"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-defensin" evidence="7">
        <location>
            <begin position="46"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU231113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005032695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="78" mass="9042" checksum="913E5C7548A243F5" modified="2022-01-19" version="1">MAPNRKMFYFFFAFFFVLAQFPSGCQAGLEYSQPFPGGEFAACEPCRLRRGKCRRMCAEDEKAVGNCKLNFFCCRQRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MZV6</accession>
    <name>A0A8C4MZV6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Beta-galactosidase</fullName>
            <ecNumber evidence="5">3.2.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GLB1L2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glycosyl hydrolase 35 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="5"/>
    <dbReference type="RefSeq" id="XP_070345985.1">
        <property type="nucleotide sequence ID" value="XM_070489884.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MZV6"/>
    <dbReference type="Ensembl" id="ENSEAST00005036372.2">
        <property type="protein sequence ID" value="ENSEASP00005033359.2"/>
        <property type="gene ID" value="ENSEASG00005022844.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106846910"/>
    <dbReference type="CTD" id="89944"/>
    <dbReference type="GeneTree" id="ENSGT00950000182942"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000049">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000293">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000036">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026283">
        <property type="entry name" value="B-gal_1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048912">
        <property type="entry name" value="BetaGal1-like_ABD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048913">
        <property type="entry name" value="BetaGal_gal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031330">
        <property type="entry name" value="Gly_Hdrlase_35_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019801">
        <property type="entry name" value="Glyco_hydro_35_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001944">
        <property type="entry name" value="Glycoside_Hdrlase_35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23421">
        <property type="entry name" value="BETA-GALACTOSIDASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21317">
        <property type="entry name" value="BetaGal_ABD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21467">
        <property type="entry name" value="BetaGal_gal-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01301">
        <property type="entry name" value="Glyco_hydro_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006336">
        <property type="entry name" value="B-gal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00742">
        <property type="entry name" value="GLHYDRLASE35"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01182">
        <property type="entry name" value="GLYCOSYL_HYDROL_F35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase 35 catalytic" evidence="7">
        <location>
            <begin position="53"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase 1-like first all-beta" evidence="8">
        <location>
            <begin position="470"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase galactose-binding" evidence="9">
        <location>
            <begin position="592"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR006336-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01301"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF21317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005033359.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="680" mass="77118" checksum="209F130AC6C0C67E" modified="2023-09-13" version="2">MTTWNLRRRPGRALGLVLLGFLTLLVLRRLDWSNLTRPWLRRRQLGLQAKDQNFMLEDSTFWIFGGSVHYFRVPKEYWRDRLLKMKACGLNTLTTYVPWNLHEPERGRFDFSGNLDLEAFVLTAAEIGLWVILRPGPYICSEIDLGGLPSWLLQDSGMRLRTTYKGFTNAVDLYFDHLMPRVVPLQYKHGGPIIAVQVENEYGSYNKDPTYMPYIKKALEDRGIEELLLTSDNKDGLSNGAVDGVLATINLQSQHDLQLLSTFLFTVQGARPKMVMEYWTGWFDSWGGTHNILDSSEVLKTVSAIIDAGSSINLYMFHGGTNFGFINGAMHYYDYKSHVTSYDYDAVLTEAGDYTAKYLQLRDFFGSISGTWPAAGSSTTMGPCFCSETPEEFFPDHPTSTPIDLCARMSWTRTGTPLPPPPDPLPKTAYESVTPAFYLSLWDALKYMEAPINSEQPVNMENLPVNNGNGQSFGYTLYETTIASSGVLSAFVRDRGQVFVNTVSIGFLDYKRKEINIPLIQGYTTLRILVENCGRVNYGEIDNQRKGLIGNIYLNDSPLSKFRIYSLDMKKTFFQRFSFDEWNKVPEAPTFPAFFLGALSVALSPSDTFMKLEGWEKGVVFINGQNLGRYWNIGPQETLYLPGTWLDQGINQVIVFEEKMAGSVIQFTETPHLGRSRYIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N069</accession>
    <name>A0A8C4N069_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ARF guanine nucleotide exchange factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ARFGEF2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Perinuclear region</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005037261.2">
        <property type="protein sequence ID" value="ENSEASP00005034177.2"/>
        <property type="gene ID" value="ENSEASG00005023348.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106825867"/>
    <dbReference type="CTD" id="10564"/>
    <dbReference type="GeneTree" id="ENSGT00940000158950"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034237">
        <property type="term" value="F:protein kinase A regulatory subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007032">
        <property type="term" value="P:endosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032760">
        <property type="term" value="P:positive regulation of tumor necrosis factor production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001881">
        <property type="term" value="P:receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00171">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000143">
        <property type="entry name" value="ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1000.11:FF:000003">
        <property type="entry name" value="Brefeldin A-inhibited guanine nucleotide-exchange protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.20:FF:000002">
        <property type="entry name" value="Brefeldin A-inhibited guanine nucleotide-exchange protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032629">
        <property type="entry name" value="DCB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015403">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032691">
        <property type="entry name" value="Mon2/Sec7/BIG1-like_HUS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046455">
        <property type="entry name" value="Sec7/BIG1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663:SF124">
        <property type="entry name" value="BREFELDIN A-INHIBITED GUANINE NUCLEOTIDE-EXCHANGE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10663">
        <property type="entry name" value="GUANYL-NUCLEOTIDE EXCHANGE FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20252">
        <property type="entry name" value="BIG2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16213">
        <property type="entry name" value="DCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09324">
        <property type="entry name" value="Sec7-like_HDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12783">
        <property type="entry name" value="Sec7-like_HUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SEC7" evidence="12">
        <location>
            <begin position="635"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="208"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="586"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="228"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="594"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="612"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005034177.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1784" mass="201519" checksum="8CCD97B29FA239A8" modified="2023-09-13" version="2">MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAEIEKQRLGAAAPPKANFIEADKYFLPFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETICNCFQGPQTDEGVQLQIIKALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEKPIQSKPQSPVIQAAAVSPKSSRLKQSQAQSKPTTPEKTDLTNGEHAGSDSGKVSSENGDAPREGGPSLPGTDDGAQEVVKEILEDVVTSAIKAAQKHRLTEPERVLGELGCQECAIPPAADENSQTNGIADDRQSLSSADNLESDSQGHQVTARFSHILQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLSVLQNAGPVFRTHEMFISAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFLNILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTPLQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQTSLGQERPTDQEMGDGKGLDMARRSSVTSMESTVSSGTQTAVQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGTSVEDIAQFLHQEERLDSTQVGDFLGDSMRFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLFASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYEEIEGKKIAMKETKEHTIATKSTKQSVASEKQRRLLYNLEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLAAYSIGLQNCDDTEVASLCLEGIRCAIRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKTLITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHTMAGEEFMGLGLGNLVTGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSLQKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDFLRPFEHIMKKNRSPTIRDMVIRCIAQMVNSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTGHIVTTIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDRVWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFEKHWWQDLFRIVFRIFDNMKLPEQQSEKSEWMTTTCNHALYAICDVFTQFYEALNEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFSPAVWDETCNCMLDIFKTTIPHVLLTWRPAGMEEDSSEKHLDVDLDRQSLSSIDKNASERGQSQLSNPTDDSWKGRPYANQKLFASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDADIHIETENQGMYKCMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDENRRDSWAEIQQRLLTVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKINDEKFKAHASMYYPYLCEIMQFDLIPELRAVLRKFFLRIGVVYKIWIPEEPSQVPGTLSPVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N3R9</accession>
    <name>A0A8C4N3R9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serpin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC106825412</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the serpin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014687635.3">
        <property type="nucleotide sequence ID" value="XM_014832149.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4N3R9"/>
    <dbReference type="Ensembl" id="ENSEAST00005034277.2">
        <property type="protein sequence ID" value="ENSEASP00005031534.2"/>
        <property type="gene ID" value="ENSEASG00005021473.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106825412"/>
    <dbReference type="KEGG" id="eai:106825412"/>
    <dbReference type="GeneTree" id="ENSGT00940000166310"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19559">
        <property type="entry name" value="serpinA14_UTMP_UABP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.497.10:FF:000001">
        <property type="entry name" value="Serine protease inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.39.10">
        <property type="entry name" value="Alpha-1-antitrypsin, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.497.10">
        <property type="entry name" value="Antithrombin, subunit I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023795">
        <property type="entry name" value="Serpin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023796">
        <property type="entry name" value="Serpin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000215">
        <property type="entry name" value="Serpin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036186">
        <property type="entry name" value="Serpin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042178">
        <property type="entry name" value="Serpin_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042185">
        <property type="entry name" value="Serpin_sf_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461">
        <property type="entry name" value="SERINE PROTEASE INHIBITOR, SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11461:SF164">
        <property type="entry name" value="UTEROFERRIN-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00079">
        <property type="entry name" value="Serpin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00093">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56574">
        <property type="entry name" value="Serpins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00284">
        <property type="entry name" value="SERPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040209978" description="Serpin domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="domain" description="Serpin" evidence="4">
        <location>
            <begin position="59"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="30"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00093"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="421" mass="48732" checksum="B5BC9B96AB76366B" modified="2023-09-13" version="2">MSHRKMHLALSLVLTLCGLLNSIFCETQPNPKRDPHPVSSRKTSPPSHEADTDHEAFAYKLFEALSVEYHRKNLIFSPESISTALAMLSLGTRSTTLSNLVAGLGFDLQRIRAWEVHTSFQRLVQTLNELDRQRHLKHRDFLFIDINRRIKPKFLQETERLYEVEAQVADFRHRGIAKEQINQFVAQRLAHRIEEVVTSLHPHTFLFLLNYIFFKGVWEVAFQARFTQKENFFLEDNTSVQVHMMRKTERMIYSRAEHLFATIVKLPYTGNVSLVLVLPDAGRFDFVAKELAARRARPLQSRDTRLVHLILPKFKISSRIDLNRLLPKVGIEDIFSRRANFSGITDETFPTIFEAIHEARLEVNEKGVIKAAAEDVHAKRAHHAPHADATVVKFDRPFLLFVEDELNQRQLFVGQVFNPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N8X9</accession>
    <name>A0A8C4N8X9_EQUAS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Gao"/>
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                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
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                <person name="G."/>
                <person name="Liu"/>
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                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
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                <person name="Zhang"/>
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                <person name="Yin"/>
                <person name="Q."/>
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                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="J."/>
                <person name="Liu"/>
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                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="322" mass="34603" checksum="FCD069FD4FCF1070" modified="2023-09-13" version="2">VHALDDVPAVVEHPADVLGVHSAGEVGVAVVAPVPTSVSTHQKFVTDEVLGPGHAWVLSGLGSTSKFWKVVLNFRFSSKDFLSEQVLLVEEQNHRDGAQPSNKEGNALEEVQGLLQAVGLVVLPDDHVVAAAGHHEDDGSHIFTLDPFAAFIALATHVKHAVGQKQHLVTPKVDFVHLELGLKDSRSQDAAAEQILKQNLVSLLDDINLVQERSGLLLGAVNQLVLVGALIAGTHSFILPQSLGGLAYRREVEVGHVHHAQDVVHSELVLRVGQLHRGHQVAHGDASTYFNGLFQVVFDVLHLGCLLTTVGLKRKKSQVATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PI87</accession>
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            <fullName evidence="5">Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3</fullName>
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            <fullName evidence="15">Membrane-associated guanylate kinase inverted 3</fullName>
        </alternativeName>
    </protein>
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        <name evidence="21" type="primary">MAGI3</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
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                <person name="Y."/>
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                <person name="G."/>
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                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the MAGUK family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005008221.2">
        <property type="protein sequence ID" value="ENSEASP00005007538.1"/>
        <property type="gene ID" value="ENSEASG00005005514.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06730">
        <property type="entry name" value="PDZ0_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06731">
        <property type="entry name" value="PDZ1_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06732">
        <property type="entry name" value="PDZ2_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06733">
        <property type="entry name" value="PDZ3_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06734">
        <property type="entry name" value="PDZ4_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06735">
        <property type="entry name" value="PDZ5_MAGI-1_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000005">
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    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000006">
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    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000012">
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    <dbReference type="FunFam" id="2.20.70.10:FF:000002">
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    <dbReference type="FunFam" id="2.30.42.10:FF:000042">
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    </dbReference>
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        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
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    <dbReference type="InterPro" id="IPR008144">
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    <dbReference type="InterPro" id="IPR027417">
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    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
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    <dbReference type="PANTHER" id="PTHR10316">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10316:SF10">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
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        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0965">Cell junction</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0796">Tight junction</keyword>
    <feature type="domain" description="PDZ" evidence="20">
        <location>
            <begin position="27"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Guanylate kinase-like" evidence="19">
        <location>
            <begin position="114"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="18">
        <location>
            <begin position="294"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="18">
        <location>
            <begin position="340"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="20">
        <location>
            <begin position="411"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="20">
        <location>
            <begin position="579"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="20">
        <location>
            <begin position="727"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="20">
        <location>
            <begin position="852"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="20">
        <location>
            <begin position="1022"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="182"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="373"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="554"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="939"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1117"/>
            <end position="1139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1171"/>
            <end position="1481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="191"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="256"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="391"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="558"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="946"/>
            <end position="956"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1171"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1194"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1222"/>
            <end position="1232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1247"/>
            <end position="1264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1308"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1353"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1374"/>
            <end position="1383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1396"/>
            <end position="1442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00033438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00056578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1481" mass="162850" checksum="0A82C2F36085E859" modified="2022-01-19" version="1">MSKTLKKKKHWLSKVQECAVSWAGPPGDLGAEIRGGAERGEFPYLGRLRDEPGGGTCCVVSGKAPSPGDVLLEVNGTPVSGLTNRDTLAVIRHFREPIRLKTVKPGKVINKDLRHYLSLQFQKGSIDHKLQQVIRDNLYLRTIPCTTRAPRDGEVPGVDYNFISVEQFKALEESGALLESGTYDGNFYGTPKPPAEPSPFQPDPVDQVLFDNEFDTESQRKRTTSVSKMERMDSSLPEEEEDEDKEAVNGSGGAVENRERHSESSDWMKTVPSYNQTNSSMDFRNYMMRDENLEPLPKNWEMAYTDTGMVYFIDHNTKTTTWLDPRLCKKAKAPEDCEDGELPYGWEKIEDPQYGTYYVDHLNQKTQFENPVDEAKRKKQLGQADTGSSKPDVEKSHFTRDPSQLKGVLLRASLKKSTMGFGFTIIGGDRPDEFLQVKNVLKDGPAAQDGKIAPGDVIVDINGNCVLGHTHADVVQMFQLVPVNQYVNLTLCRGYPLPDENEDPVVDIVAATPVINGQSLTKGETCMNTQDFKSGAMVLDQNGKSGHTLIGDRLNGLSDPSEQRASMASSGSSQPELVTIPLIKGPKGFGFAIADSPTGQKVKMILDSQWCQGLQKGDIIKEIYHQNVQNLTHLQVVEVLKQFPVGADVPLLILRGGPPSPTKTAKMKTDKKENSGSLEAINEPIPQPMPFPPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRVLGGDGPDQPIYIGAIIPLGAAEKDGRLRAADELMCIDGIPVKGKSHKQVLDLMTTAARNGHVLLTVRRKIFYGEKQLEDDSSQAFISTHNGSPRLNRAEIPARPPPQEAYDVVLQRKENEGFGFVILTSKSKPPPGVIPHKIGRVIEGSPADRCGKLKVGDHISAVNGQSIIELSHDSIVQLIKDAGITVTLTVVADEEHHGPPSGTNSARQSPALQHRPMGQSQANHIPGDRSALEGEIGKDVSTSYRHSWSDHRPLAQPDTAMISVVGSRHNQSLGCYPVELERGPRGFGFSLRGGKEYNMGLFILRLAEEGPAIKDGRIHVGDQIVEINGEPTQGITHTRAIELIQAGGNKVLLLLRPGTGLIPDHGDWDMNSPSSSSVIYDEQSPFPPSPHSASMFEESHVPVIEESLMRVQICEKAEELRDTVHEKKSTLNEKQSDIKHQSLLQKNVNKRDPPNSLGHSDKRNLLKVETGIPQRGRSASPQKSASRYSEEHLEKIPSPLKNEPKTRPRDRSLSPRKGENSGQPGARAGSGQEHSRQGRGQSSSPKKQQKTEGSKDQSSAEAKLLELESRRGGGRARDRAERVWDGKEKAGVLRKDMKQSQSGKNRTRSPEEKSKRMDEKSLLAKKTDNTTSRAASENDQGRKATSGETSSSKDKIGESVKVSEKRKQEPEERMVLNKPEDHKGKELEAADKNRESGGFKPESGSPAKKTPITPGPWKVPSAHRATGTTGMAEKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PQP3</accession>
    <name>A0A8C4PQP3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">Ferroptosis suppressor protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="24">Apoptosis-inducing factor homologous mitochondrion-associated inducer of death</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="25">p53-responsive gene 3 protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">AIFM2</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>menadione + NADH + H(+) = menadiol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:69695"/>
            <dbReference type="ChEBI" id="CHEBI:6746"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28869"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:69696"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>menaquinone-4 + NADH + H(+) = menaquinol-4 + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:74079"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:78277"/>
            <dbReference type="ChEBI" id="CHEBI:193091"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:74080"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>phylloquinone + NADH + H(+) = phylloquinol + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:74075"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18067"/>
            <dbReference type="ChEBI" id="CHEBI:28433"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:74076"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>ubiquinone-10 + NADH + H(+) = ubiquinol-10 + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:61984"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:46245"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64183"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:61985"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="22">
            <name>6-hydroxy-FAD</name>
            <dbReference type="ChEBI" id="CHEBI:60470"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="30">Interacts with importin subunits KPNA2 and IPO5; this interaction likely mediates the translocation into the nucleus upon oxidative stress.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070345441.1">
        <property type="nucleotide sequence ID" value="XM_070489340.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070345446.1">
        <property type="nucleotide sequence ID" value="XM_070489345.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070345449.1">
        <property type="nucleotide sequence ID" value="XM_070489348.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070345451.1">
        <property type="nucleotide sequence ID" value="XM_070489350.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070345454.1">
        <property type="nucleotide sequence ID" value="XM_070489353.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070345458.1">
        <property type="nucleotide sequence ID" value="XM_070489357.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PQP3"/>
    <dbReference type="Ensembl" id="ENSEAST00005025594.2">
        <property type="protein sequence ID" value="ENSEASP00005023588.1"/>
        <property type="gene ID" value="ENSEASG00005016041.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106845022"/>
    <dbReference type="CTD" id="84883"/>
    <dbReference type="GeneTree" id="ENSGT00390000004582"/>
    <dbReference type="OMA" id="TWEIAPP"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008637">
        <property type="term" value="P:apoptotic mitochondrial changes"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="Apoptosis-inducing factor, mitochondrion-associated, 2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43735:SF3">
        <property type="entry name" value="FERROPTOSIS SUPPRESSOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0007">Acetylation</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0551">Lipid droplet</keyword>
    <keyword evidence="21" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="19" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-0519">Myristate</keyword>
    <keyword evidence="20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="31">
        <location>
            <begin position="13"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00037027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00040253"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00041541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00042318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00049236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00049275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00049479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00066050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005023588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="373" mass="40651" checksum="1968D2A45A996743" modified="2022-01-19" version="1">MGSQVSMDVGAVHVVIVGGGFGGIVAASKLQALNIPFVLVDMKDSFHHNVAALRASVESGFAKKTFISYSVTFRESFRQGLVVEIDLKNQTVLLEDGEALPFSHLILATGSTGPFPGKFNQVSSQQVAIQLYEDMVTQVQRAQSIVVVGGGSAGVEMAAEIKTDYPEKEVTLIHSQMALADKELLPCVRQEVKEILLRKGVQLLLSERVSNLEELPFNEYRECIKVQTDKGTEVATNLVIVCNGIKINSFAYRSAFDSHLASTGALRVNKYLQVEGYSHIYAIGDCADVKEPKMAYHASLHANVAVANIINSMQQRPLKAYVPGSLTFLLALGRNDGVGQISGFYVGRLMVRLAKSRDLLVSTSWKTMRQCPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I8G0</accession>
    <name>A0A9L0I8G0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Lysophospholipase-like protein 1</fullName>
            <ecNumber evidence="3">3.1.2.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LYPLAL1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Palmitoyl thioesterase that catalyzes depalmitoylation of CGAS and KCNMA1. Acts as a regulator of innate immunity by mediating depalmitoylation of CGAS, thereby preventing CGAS homodimerization and cyclic GMP-AMP synthase activity. Does not exhibit phospholipase nor triacylglycerol lipase activity, able to hydrolyze only short chain substrates due to its shallow active site.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:19234"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0I8G0"/>
    <dbReference type="Ensembl" id="ENSEAST00005053021.1">
        <property type="protein sequence ID" value="ENSEASP00005036832.1"/>
        <property type="gene ID" value="ENSEASG00005003523.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159171"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140896">
        <property type="term" value="P:cGAS/STING signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160049">
        <property type="term" value="P:negative regulation of cGAS/STING signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000139">
        <property type="entry name" value="lysophospholipase-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050565">
        <property type="entry name" value="LYPA1-2/EST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655:SF17">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="10">
        <location>
            <begin position="14"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005036832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="239" mass="26572" checksum="A8B6924AF08FEF2F" modified="2023-09-13" version="1">MAAAWGSVRLQRCMVSPAGRHSASLIFLHGSGDSGQGLRTWIKQVLNQDLTFQHIKIIYPTAPPRPYTPLEGSISNVWFDRYKISNDCPEHLESINGMCQVLTDLIDDEVKSGIKKNRILVGGFSMGGCMAMHLAYRNHQDVAGVFALSSFLNKTSAVYQALQKSDGVLPELFQCHGTADELVLHCWGEETNTMLKSLGVSTQFHSFPGLCHELSKAELEKLKSWVLTKLPGEMERHNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IAK5</accession>
    <name>A0A9L0IAK5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zinc finger protein 575</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ZNF575</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044614923.1">
        <property type="nucleotide sequence ID" value="XM_044758988.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044614924.1">
        <property type="nucleotide sequence ID" value="XM_044758989.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044614925.1">
        <property type="nucleotide sequence ID" value="XM_044758990.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044614926.1">
        <property type="nucleotide sequence ID" value="XM_044758991.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IAK5"/>
    <dbReference type="Ensembl" id="ENSEAST00005063443.1">
        <property type="protein sequence ID" value="ENSEASP00005034739.1"/>
        <property type="gene ID" value="ENSEASG00005036438.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106848400"/>
    <dbReference type="KEGG" id="eai:106848400"/>
    <dbReference type="CTD" id="284346"/>
    <dbReference type="GeneTree" id="ENSGT00940000162551"/>
    <dbReference type="OMA" id="AAHRWTH"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000565">
        <property type="entry name" value="Zinc finger protein 575"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226">
        <property type="entry name" value="ZINC FINGER AND SCAN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23226:SF206">
        <property type="entry name" value="ZINC FINGER PROTEIN 575"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="64"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="92"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="178"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="15">
        <location>
            <begin position="214"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00068599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005034739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="246" mass="26529" checksum="5A47D3F7164B478B" modified="2023-09-13" version="1">MLERDAESAAGDTDPRPAGKEPVTKGGAPHQGPPQTPSQSVPGPALSAGAPSRPRRRPPPQRPHRCPDCDKAFSYPSKLATHRLAHGGARPHPCPDCPKAFSYPSKLAAHRLTHSGARPHPCPHCPKAFGHRSKLAAHLWTHAPARPYPCPDCPKSFCYPSKLAAHRHTHHATDARPYPCPHCPKAFSFPSKLAAHRLCHDPPTAPGSQPAARHHCSSCGQAFGQKRLLLLHQRSHPAAESQGEPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0ID73</accession>
    <name>A0A9L0ID73_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15 16">Biliverdin reductase A</fullName>
            <shortName evidence="16">BVR A</shortName>
            <ecNumber evidence="14 16">1.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">BLVRA</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11 16">Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IXalpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor. Does not reduce bilirubin IXbeta. Uses the reactants NADH or NADPH depending on the pH; NADH is used at the acidic pH range (6-6.9) and NADPH at the alkaline range (8.5-8.7). NADPH, however, is the probable reactant in biological systems.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(4Z,15Z)-bilirubin IXalpha + NAD(+) = biliverdin IXalpha + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15797"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:57977"/>
            <dbReference type="ChEBI" id="CHEBI:57991"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>(4Z,15Z)-bilirubin IXalpha + NADP(+) = biliverdin IXalpha + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57977"/>
            <dbReference type="ChEBI" id="CHEBI:57991"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="18">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12 16">Porphyrin-containing compound metabolism; protoheme degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 16">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 16">Cytoplasm</location>
            <location evidence="1 16">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 16">Belongs to the Gfo/Idh/MocA family. Biliverdin reductase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.24" evidence="14 16"/>
    <dbReference type="RefSeq" id="XP_014709208.2">
        <property type="nucleotide sequence ID" value="XM_014853722.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070360418.1">
        <property type="nucleotide sequence ID" value="XM_070504317.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070360425.1">
        <property type="nucleotide sequence ID" value="XM_070504324.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0ID73"/>
    <dbReference type="Ensembl" id="ENSEAST00005061831.1">
        <property type="protein sequence ID" value="ENSEASP00005038161.1"/>
        <property type="gene ID" value="ENSEASG00005017707.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106839159"/>
    <dbReference type="CTD" id="644"/>
    <dbReference type="GeneTree" id="ENSGT00390000011072"/>
    <dbReference type="OMA" id="IQVAFIC"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106276">
        <property type="term" value="F:biliberdin reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042167">
        <property type="term" value="P:heme catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000020">
        <property type="entry name" value="Biliverdin reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000179">
        <property type="entry name" value="Biliverdin reductase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017094">
        <property type="entry name" value="Biliverdin_Rdtase_A"/>
    </dbReference>
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        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
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        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3 16" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 16 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 16" id="KW-0520">NAD</keyword>
    <keyword evidence="7 16 17" id="KW-0521">NADP</keyword>
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    <keyword evidence="6 16 18" id="KW-0862">Zinc</keyword>
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        <location>
            <begin position="9"/>
            <end position="125"/>
        </location>
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        <location>
            <begin position="133"/>
            <end position="243"/>
        </location>
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        <location>
            <begin position="16"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:58349"/>
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            <begin position="44"/>
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        <location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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        <source>
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    </evidence>
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            <fullName evidence="3">sphingomyelin phosphodiesterase</fullName>
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                <person name="Huang"/>
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                <person name="Zhao"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Li"/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <text evidence="1">Lipid metabolism; sphingolipid metabolism.</text>
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        <text evidence="2">Sphingolipid metabolism.</text>
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        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE 5"/>
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        <property type="entry name" value="DNase I-like"/>
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    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
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            <begin position="26"/>
            <end position="50"/>
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            <begin position="71"/>
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            <begin position="301"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="500"/>
            <end position="519"/>
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    </feature>
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    </evidence>
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    </evidence>
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            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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</entry>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Z."/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
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                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Zhuang"/>
                <person name="G."/>
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                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
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                <person name="J."/>
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                <person name="R."/>
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                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
                <person name="Q."/>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(R)-3-hydroxybutanoate + NAD(+) = acetoacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20521"/>
            <dbReference type="ChEBI" id="CHEBI:10983"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.30"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Siderophore biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.104" evidence="6"/>
    <dbReference type="EC" id="1.1.1.30" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0II28"/>
    <dbReference type="Ensembl" id="ENSEAST00005046047.1">
        <property type="protein sequence ID" value="ENSEASP00005038333.1"/>
        <property type="gene ID" value="ENSEASG00005037239.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005051604.1">
        <property type="protein sequence ID" value="ENSEASP00005040533.1"/>
        <property type="gene ID" value="ENSEASG00005031037.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005056735.1">
        <property type="protein sequence ID" value="ENSEASP00005062235.1"/>
        <property type="gene ID" value="ENSEASG00005028214.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005061591.1">
        <property type="protein sequence ID" value="ENSEASP00005041132.1"/>
        <property type="gene ID" value="ENSEASG00005029137.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005065651.1">
        <property type="protein sequence ID" value="ENSEASP00005045503.1"/>
        <property type="gene ID" value="ENSEASG00005031221.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005066634.1">
        <property type="protein sequence ID" value="ENSEASP00005057279.1"/>
        <property type="gene ID" value="ENSEASG00005024511.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005078782.1">
        <property type="protein sequence ID" value="ENSEASP00005058016.1"/>
        <property type="gene ID" value="ENSEASG00005033852.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156721"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003858">
        <property type="term" value="F:3-hydroxybutyrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016617">
        <property type="term" value="F:4-oxoproline reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019290">
        <property type="term" value="P:siderophore biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051122">
        <property type="entry name" value="SDR_DHRS6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43477:SF4">
        <property type="entry name" value="DEHYDROGENASE_REDUCTASE SDR FAMILY MEMBER 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43477">
        <property type="entry name" value="DIHYDROANTICAPSIN 7-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00042565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00043083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005040533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005041132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="67" mass="7164" checksum="508286D1FC59C84B" modified="2023-09-13" version="1">MGRLDGKVIVLTAAAQGIGRATAIAFAREGAQVIATDINESKLQELEKYPGKQLSSLNLGFRVHKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IK71</accession>
    <name>A0A9L0IK71_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">OTU deubiquitinase with linear linkage specificity like</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">OTULINL</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C65 family. Otulin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IK71"/>
    <dbReference type="Ensembl" id="ENSEAST00005042513.1">
        <property type="protein sequence ID" value="ENSEASP00005040547.1"/>
        <property type="gene ID" value="ENSEASG00005017778.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009802"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098554">
        <property type="term" value="C:cytoplasmic side of endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd22798">
        <property type="entry name" value="OTU_OTULL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023235">
        <property type="entry name" value="FAM105"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023236">
        <property type="entry name" value="OTULINL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33662:SF1">
        <property type="entry name" value="INACTIVE UBIQUITIN THIOESTERASE OTULINL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33662">
        <property type="entry name" value="OTU DEUBIQUITINASE WITH LINEAR LINKAGE-SPECIFICITY A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16218">
        <property type="entry name" value="Peptidase_C101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02055">
        <property type="entry name" value="PROTEINF105"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02056">
        <property type="entry name" value="PROTEINF105A"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="9"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005040547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="423" mass="48774" checksum="5985361BBE597525" modified="2023-09-13" version="1">MGRRGRGQPGRAPSAGRRLGPGFGVRGPRPGRRGAPEQGEEATGCTPHAPCRVGAAVEARGASHHGTTPSRAEPFGSRQRANPQGPQGGNDQVHAWTLVTSQALDTAWRIAKGSVMLAVSFLVAALCYFKRLHLYLGRRLKWWIGYLQRKFKRNLSVEAEVDLLSYCAREWKGETPRAKLMRKAYEELFWRRHIKCVRQVKRDNYDALRSVLFQIFSQGLSFPSWMKEKDIVKLPEKLLFSQGCNWIQQYSFGPEKYTGSNVFGKLRKCVELLKTQWTEFSGIKDYHKRGSMCNILFSNAILEYKLYEALKFIMLYQVTEVYEQMKTKKVIPSLFRLLFSRETSCDPLSFMMNHLNSVGDTCGLEQIDMFILGYSLEVKIKVFRLFKFNSRDFEVCYPEEPLREWPEISLLTENDRHYHIPVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IKL2</accession>
    <name>A0A9L0IKL2_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small nuclear RNA activating complex polypeptide 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">SNAPC1</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <begin position="362"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="377" mass="43799" checksum="5C7CE3C515F04156" modified="2023-09-13" version="1">MGAPPGLQADCEALLSRFQETDSVRFEDFTELWRSMKFATIFCGRMRNLEKNTFTKEALALAWRYFLPPYTFQIRVGALYLLYGLYNTQLCQPKQKIRVALKDWDEILKFQQDLINAQHFDAAYIFRKLRLDKAFHFTAMPKLLSYRMKKKIQRAEVTEEFKDPNDRVMKLITSDVLEEMLNVHDHYQNMKHIISADKSNPDKALSLIKDDFFDNIKNIVLEHQQWHKDRKNPSLKSKGKDGEEKREGNSQESERCERAESLAKIKSKAFSVVVQASKSRRHRQVKLDSSDSDSASGQGQIKATRKKGSQDTLKSAGRKMSSRNRDDMQNVHKEDKSLSLSMPVITEEEEENESSETEFTALKRKRKHRTKSLVSSS</sequence>
</entry>
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    <accession>A0A9L0IN33</accession>
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        <recommendedName>
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        </recommendedName>
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        <name evidence="11" type="primary">CAMKV</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Eutheria</taxon>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
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                <person name="Y."/>
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                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="Y."/>
                <person name="Gao"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
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                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="J."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <cofactor evidence="1">
            <name>Ca(2+)</name>
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        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with calmodulin, in the presence of calcium.</text>
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    <comment type="subcellular location">
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            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050804">
        <property type="term" value="P:modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099159">
        <property type="term" value="P:regulation of modification of postsynaptic structure"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14088">
        <property type="entry name" value="STKc_CaMK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000188">
        <property type="entry name" value="CaM kinase-like vesicle-associated, like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CaM kinase-like vesicle-associated, like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="24"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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        </location>
    </feature>
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        </source>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00006692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022860"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="473" mass="51945" checksum="FB0E6840B714CEC6" modified="2023-09-13" version="1">MPFGCVTLGDKKNYNQPSEVTDRYDLGQVIKTEEFCEIFRAKDKTTGKLHTCKKFQKRDGRKVRKAAKNEIGILKMVKHPNILQLVDVFVTRKEYFIFLELATGREVFDWILDQGYYSERDTSNVVRQVLEAVAYLHSLKIVHRNLKLENLVYYNRLKNSKIVISDFHLAKLENGLIKEPCGTPEYLAPEVVGRQRYGRPVDCWAIGVIMYILLSGNPPFYEEVEEDDYENHDKNLFRKILAGDYEFDSPYWDDISQAAKDLVTRLMEVEQDQRITAEEAISHEWISGNAASDKNIKDGVCAQIEKNFARAKWKKAVRVTTLMKRLRAPEQSSTAAAPSAPATDTATPGATAPADRSATPATDGSTTPATDGSITPATDGSITPATDGSVTPATDRSVTPATDGRATPATEESTVPTTQSSVTLATKAAATPEPALAQPDSTAPGGTTGQAPPSSKGEEAAGYAQESQRVETS</sequence>
</entry>
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    <accession>A0A9L0INY8</accession>
    <name>A0A9L0INY8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Li"/>
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                <person name="Li"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
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                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
                <person name="J."/>
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                <person name="M."/>
                <person name="Wu"/>
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                <person name="X. and Zhong"/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSEASG00005031954.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286925"/>
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        <property type="component" value="Chromosome 25"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="146" mass="16941" checksum="C1A4722D477DCEDF" modified="2023-09-13" version="1">FILLQGCEDWRIPGEKHAALVIEGRNSRELEPIHTSIFKVSAWTWYRSHLLTSIVVRPSPKSVGGENTLPKGKQVRLLALKSTLTFEWLYQISSVSISRVRCTPMFIAALFTIAKTWNQPTCPETDDWIKKMWYIYTMEYYSAIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IRF9</accession>
    <name>A0A9L0IRF9_EQUAS</name>
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        <recommendedName>
            <fullName evidence="12">Amine oxidase</fullName>
            <ecNumber evidence="12">1.4.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106826525</name>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <cofactor evidence="12">
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </cofactor>
        <text evidence="12">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>L-topaquinone</name>
            <dbReference type="ChEBI" id="CHEBI:79027"/>
        </cofactor>
        <text evidence="11">Contains 1 topaquinone per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="11 12">Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the copper/topaquinone oxidase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.3.-" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0IRF9"/>
    <dbReference type="Ensembl" id="ENSEAST00005079921.1">
        <property type="protein sequence ID" value="ENSEASP00005040371.1"/>
        <property type="gene ID" value="ENSEASG00005010057.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183207"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008131">
        <property type="term" value="F:primary methylamine oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009308">
        <property type="term" value="P:amine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046677">
        <property type="term" value="P:response to antibiotic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.40:FF:000001">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.20">
        <property type="entry name" value="Copper amine oxidase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049948">
        <property type="entry name" value="Cu_Am_ox_TPQ-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000269">
        <property type="entry name" value="Cu_amine_oxidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015798">
        <property type="entry name" value="Cu_amine_oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036460">
        <property type="entry name" value="Cu_amine_oxidase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016182">
        <property type="entry name" value="Cu_amine_oxidase_N-reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015802">
        <property type="entry name" value="Cu_amine_oxidase_N3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638">
        <property type="entry name" value="COPPER AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10638:SF23">
        <property type="entry name" value="MEMBRANE PRIMARY AMINE OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01179">
        <property type="entry name" value="Cu_amine_oxid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02728">
        <property type="entry name" value="Cu_amine_oxidN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00766">
        <property type="entry name" value="CUDAOXIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49998">
        <property type="entry name" value="Amine oxidase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54416">
        <property type="entry name" value="Amine oxidase N-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01164">
        <property type="entry name" value="COPPER_AMINE_OXID_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0106">Calcium</keyword>
    <keyword evidence="7 12" id="KW-0186">Copper</keyword>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 12" id="KW-0801">TPQ</keyword>
    <feature type="domain" description="Copper amine oxidase N3-terminal" evidence="14">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="domain" description="Copper amine oxidase catalytic" evidence="13">
        <location>
            <begin position="105"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via topaquinone" evidence="10">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <feature type="modified residue" description="2',4',5'-topaquinone" evidence="11">
        <location>
            <position position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR600269-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005040371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="466" mass="52313" checksum="D6A60344AE478D69" modified="2023-09-13" version="1">MNTAPRGLQSGDRATWFGLYYNISGAGFFLHPVGLELLVDHKALDPAHWTIRKVFFQGRYYESLAQLEDQFEAGLVNVVLVPDNGTGGSWSLKSQVPPGPAPPLQVHPEGPRFSVQGNRVVSSLWTFSFGLGAFSGPRIFDIRFQGERIAYELSLQEALAVYGGNSPSSLRSRYIDGGFGLGHFSSPLTHGVDCPYLATYMDWHFLLESQDPKTIHDAFCVFEQNQGLPLRRHHSDIHSHYFGGLAETVLVVRSVSTMLNYDYVWDMVFHPNGAIEVKFHATGYISSAFLFGAARRYGNQVGEHTLGTIHTHSAHFKVDLDAGGLENWVWAEDMAFDLTSVPWSPEHQIQRLRVTQKLLETEEQAAFPLGGTHPRYLYLASNHSNKWGHPRGYRIQMLSFAGEPLPRNSSMERAFSWGRYQLAVTQRKEGEPSSTSIYNLNDPWTPTVDFTDFINNETVAGTWWPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IRN8</accession>
    <name>A0A9L0IRN8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 13">Bis(5'-adenosyl)-triphosphatase</fullName>
            <ecNumber evidence="13">3.6.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">FHIT</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 13">
            <text>P(1),P(3)-bis(5'-adenosyl) triphosphate + H2O = AMP + ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13893"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.1.29"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>adenosine 5'-phosphoramidate + H2O = NH4(+) + AMP</text>
            <dbReference type="Rhea" id="RHEA:67916"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57890"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>adenosine 5'-phosphosulfate + H2O = sulfate + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:17041"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>adenosine 5'-phosphosulfate + NH4(+) = adenosine 5'-phosphoramidate + sulfate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:19197"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57890"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="EC" id="2.7.7.51"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Interacts with UBE2I. Interacts with MDM2. Interacts with CTNNB1. Identified in a complex with CTNNB1 and LEF1.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.29" evidence="13"/>
    <dbReference type="RefSeq" id="XP_014724237.1">
        <property type="nucleotide sequence ID" value="XM_014868751.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070348870.1">
        <property type="nucleotide sequence ID" value="XM_070492769.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070348871.1">
        <property type="nucleotide sequence ID" value="XM_070492770.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070348872.1">
        <property type="nucleotide sequence ID" value="XM_070492771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IRN8"/>
    <dbReference type="SMR" id="A0A9L0IRN8"/>
    <dbReference type="Ensembl" id="ENSEAST00005080111.1">
        <property type="protein sequence ID" value="ENSEASP00005040451.1"/>
        <property type="gene ID" value="ENSEASG00005034025.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106848673"/>
    <dbReference type="KEGG" id="eai:106848673"/>
    <dbReference type="CTD" id="2272"/>
    <dbReference type="GeneTree" id="ENSGT00510000047967"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047627">
        <property type="term" value="F:adenylylsulfatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047352">
        <property type="term" value="F:adenylylsulfate-ammonia adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047710">
        <property type="term" value="F:bis(5'-adenosyl)-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015964">
        <property type="term" value="P:diadenosine triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072332">
        <property type="term" value="P:intrinsic apoptotic signaling pathway by p53 class mediator"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032435">
        <property type="term" value="P:negative regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006163">
        <property type="term" value="P:purine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01275">
        <property type="entry name" value="FHIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000011">
        <property type="entry name" value="Fragile histidine triad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052677">
        <property type="entry name" value="Dinucleoside_ppp_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039383">
        <property type="entry name" value="FHIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46981">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46981:SF1">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="14">
        <location>
            <begin position="2"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="12">
        <location>
            <begin position="94"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="9">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="89"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for induction of apoptosis" evidence="11">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00065296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU366076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005040451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="149" mass="17076" checksum="0A9454B99B556620" modified="2023-09-13" version="1">MSFRFGQHLIKPSVVFLKTELSFALVNRKPVVPGHVLVCPLRPVERFRDLRPEEVADLFQATQRVGTVVEKHFQGTSLTFSMQDGPEAGQTVKHVHIHVLPRKAGDFHRNDSIYEELQKHDKEEEDSPALWRSEEDMAAEAAALRVYFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IRQ3</accession>
    <name>A0A9L0IRQ3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106832652</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IRQ3"/>
    <dbReference type="Ensembl" id="ENSEAST00005059192.1">
        <property type="protein sequence ID" value="ENSEASP00005039769.1"/>
        <property type="gene ID" value="ENSEASG00005035710.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282532"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15912">
        <property type="entry name" value="7tmA_OR6C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000013">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047132">
        <property type="entry name" value="Olfact_rcpt_6C-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26454:SF39">
        <property type="entry name" value="OLFACTORY RECEPTOR 6C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
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            <begin position="49"/>
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        <source>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="322" mass="36669" checksum="6EEBE961FA7A6A5C" modified="2023-09-13" version="1">MRNFVIKNSLMWSFLSSEFFILLGLSDDPDLQIVIFLFLFIMYVLSVTGNLAIITLTLVDSHLQTPMYFFLRNFSFLEISFTTVCIPRFLGAIITRDKTISYNNCAAQLFFFIFMGVTEFYILTAMSYDRYVAICKPLHYTTIMNRKLCSLLVLCAWLGGFLTIFPPLMLLLQCDYCASNVIDHFACDYFPLLQLSCSDTWLLEVIGFYFALVTLLITLALVVLSYMYIIRTILRIPSASQRKKAFSTCSSHMIVISISYGSCIFMYANPTAKEKASLTKGVAILNTSVAPMLNPFIYTLRNQQVKQAFKDVVHKVAFSATK</sequence>
</entry>
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    <accession>A0A9L0IUT8</accession>
    <name>A0A9L0IUT8_EQUAS</name>
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        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
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                <person name="Wang"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Yu"/>
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                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IUT8"/>
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        <property type="gene ID" value="ENSEASG00005024805.1"/>
    </dbReference>
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        <property type="component" value="Chromosome 24"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052251">
        <property type="entry name" value="GH-ZnFinger_Regulators"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057986">
        <property type="entry name" value="TPR_Rlf/292/654"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15507:SF14">
        <property type="entry name" value="ZINC FINGER PROTEIN 292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15507">
        <property type="entry name" value="ZINC FINGER PROTEIN RLF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TPR_Rlf"/>
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    </dbReference>
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        <property type="entry name" value="zf-C2H2_ZN292"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="219"/>
            <end position="246"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00003767"/>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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    <accession>A0A9L0IY83</accession>
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        <recommendedName>
            <fullName evidence="5">Solute carrier family 66 member 3</fullName>
        </recommendedName>
    </protein>
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        <name evidence="3" type="primary">SLC66A3</name>
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            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="S."/>
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                <person name="Y."/>
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                <person name="Z."/>
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                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IY83"/>
    <dbReference type="Ensembl" id="ENSEAST00005055909.1">
        <property type="protein sequence ID" value="ENSEASP00005045073.1"/>
        <property type="gene ID" value="ENSEASG00005034683.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000153916"/>
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        <property type="component" value="Chromosome 6"/>
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        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR016817">
        <property type="entry name" value="MannP-dilichol_defect-1"/>
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    <dbReference type="PANTHER" id="PTHR12226">
        <property type="entry name" value="MANNOSE-P-DOLICHOL UTILIZATION DEFECT 1 LEC35 -RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR12226:SF3">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 66 MEMBER 3"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005045073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="293" mass="32269" checksum="73AB7AEA6FA86C07" modified="2023-09-13" version="1">GPGGQGPRAGKAEGLGEPGGPVSTGCSWSCDPTLRAPRPRPRPRPVRRRHVGGNAGRLQLEHPGRVRRAEAAADLRRADGAQRAGHQPAEPAAGAGGASTTISWVLQTILCSWVRRGLRAVSAESSSSSELRGALPLHRFLVFLRYQCYYEYPLLTYLEYPILILQDLILLLCVFHFNGDVRRAAPYIALLVTSWFLLTLQKWIIDLAINLCTFISAASKFAQLQDLWKTKDSGVMSALTCSLASYTCATRIITTLMTTNDLTILTRFVIMMTLNIWVTATVLRYQKTDVKAE</sequence>
</entry>
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    <accession>A0A9L0J051</accession>
    <name>A0A9L0J051_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transmembrane protein 14C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TMEM14C</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Required for normal heme biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM14 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014700066.1">
        <property type="nucleotide sequence ID" value="XM_014844580.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014700073.1">
        <property type="nucleotide sequence ID" value="XM_014844587.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014700081.1">
        <property type="nucleotide sequence ID" value="XM_014844595.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J051"/>
    <dbReference type="Ensembl" id="ENSEAST00005072264.1">
        <property type="protein sequence ID" value="ENSEASP00005046621.1"/>
        <property type="gene ID" value="ENSEASG00005035461.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106833388"/>
    <dbReference type="KEGG" id="eai:106833388"/>
    <dbReference type="CTD" id="51522"/>
    <dbReference type="GeneTree" id="ENSGT00940000154772"/>
    <dbReference type="OMA" id="ANSHKIM"/>
    <dbReference type="OrthoDB" id="5620at2759"/>
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        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070453">
        <property type="term" value="P:regulation of heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1740:FF:000002">
        <property type="entry name" value="Transmembrane protein 14C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1740">
        <property type="entry name" value="Transmembrane protein 14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005349">
        <property type="entry name" value="TMEM14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044890">
        <property type="entry name" value="TMEM14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 14, 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 14C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03647">
        <property type="entry name" value="Tmemb_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="89"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005046621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="115" mass="11823" checksum="CBD99D589907B70F" modified="2023-09-13" version="1">MQKDSGPLVPLHWIGFGYAALIASGGIIGYAKAGSVPSLAAGLLFGGLAGLGAYQLSQDPRNIWVFLATSGTLAGIMGMRFYHSGKFMPAGLIAGASLLMVAKLGISVFSRPHQS</sequence>
</entry>
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    <accession>A0A9L0J0P9</accession>
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            <fullName evidence="18">E3 SUMO-protein ligase PIAS4</fullName>
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        <alternativeName>
            <fullName evidence="21">Protein inhibitor of activated STAT protein 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Protein inhibitor of activated STAT protein gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">PIAS4</name>
    </gene>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein sumoylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with AR, GATA2, LEF1, TP53 and STAT1 (IFNG-induced). Interacts with TICAM1. Interacts with MTA1. Interacts with PRDM1/Blimp-1. Interacts with TRIM32 upon treatment with UVB and TNF-alpha.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Nucleus</location>
            <location evidence="16">Nuclear body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIAS family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044609336.1">
        <property type="nucleotide sequence ID" value="XM_044753401.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J0P9"/>
    <dbReference type="Ensembl" id="ENSEAST00005076830.1">
        <property type="protein sequence ID" value="ENSEASP00005046709.1"/>
        <property type="gene ID" value="ENSEASG00005022327.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106832367"/>
    <dbReference type="KEGG" id="eai:106832367"/>
    <dbReference type="CTD" id="51588"/>
    <dbReference type="GeneTree" id="ENSGT01030000234539"/>
    <dbReference type="OMA" id="TPIVECK"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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</entry>
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    <accession>A0A9L0J568</accession>
    <name>A0A9L0J568_EQUAS</name>
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            <fullName evidence="13">Vomeronasal type-1 receptor</fullName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative pheromone receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J568"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0019236">
        <property type="term" value="P:response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:sensory perception of chemical stimulus"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Vomeronasal type-1 receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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    <dbReference type="InterPro" id="IPR004072">
        <property type="entry name" value="Vmron_rcpt_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24062">
        <property type="entry name" value="VOMERONASAL TYPE-1 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03402">
        <property type="entry name" value="V1R"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="VOMERONASL1R"/>
    </dbReference>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="47"/>
            <end position="67"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="129"/>
            <end position="151"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="171"/>
            <end position="196"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="228"/>
            <end position="250"/>
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        <location>
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            <end position="289"/>
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            <begin position="22"/>
            <end position="278"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00003878"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022507"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023040"/>
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            <dbReference type="ARBA" id="ARBA00023170"/>
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            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU364061"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50262"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005047513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="299" mass="34020" checksum="B95AEA74296B2E74" modified="2023-09-13" version="1">MFSSDTILGIFLISQICIGFLGNSLLFVLHMYTFLAQSHLKKPIDLIFIHLTLANVLTMMLKLIPHVAASFGIRHFLDDLGCKVTLYIYRVTRGFSICITSLLSAFQAITISPSNSKWARLKSKLSTCIFPSFLFFWIINMLIYTNIIQTVGANRNFTIVGSGHSKGQPTVLFLSIIVIRDLLFVVLMMESSIYMVNLLYKHRQRAQHVHSPSVSSQPSPEMKATHNILLLVSCFVFFYCAHNFSTLYFFYRPEKNLRLERIPGIISACYPTICPFVLMKISGVIFTLFGSVRLNLFFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J5J3</accession>
    <name>A0A9L0J5J3_EQUAS</name>
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        <recommendedName>
            <fullName evidence="2">Nuclear speckle splicing regulatory protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Coiled-coil domain-containing protein 55</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NSRP1</name>
    </gene>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NSRP1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044600656.1">
        <property type="nucleotide sequence ID" value="XM_044744721.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J5J3"/>
    <dbReference type="Ensembl" id="ENSEAST00005042938.1">
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        <property type="gene ID" value="ENSEASG00005009692.2"/>
    </dbReference>
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    <dbReference type="CTD" id="84081"/>
    <dbReference type="GeneTree" id="ENSGT00940000154049"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046850">
        <property type="entry name" value="NRP1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042816">
        <property type="entry name" value="Nsrp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018612">
        <property type="entry name" value="NSRP1_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31938">
        <property type="entry name" value="NUCLEAR SPECKLE SPLICING REGULATORY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31938:SF4">
        <property type="entry name" value="NUCLEAR SPECKLE SPLICING REGULATORY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="NSRP1_N"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nuclear speckle splicing regulatory protein 1 N-terminal" evidence="7">
        <location>
            <begin position="62"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="domain" description="Nuclear speckle splicing regulatory protein 1 RS-like" evidence="8">
        <location>
            <begin position="295"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
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            <begin position="22"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="197"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="110"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="205"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
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            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
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        <location>
            <begin position="311"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="346"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="362"/>
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        </location>
    </feature>
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        <location>
            <begin position="453"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="506"/>
            <end position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010126"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00020556"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="5">Ig-like domain-containing protein</fullName>
        </recommendedName>
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        <name type="scientific">Equus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
                <person name="J."/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
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                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J5W4"/>
    <dbReference type="Ensembl" id="ENSEAST00005045613.1">
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        <property type="gene ID" value="ENSEASG00005035156.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234540"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
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        <location>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
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    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="102" mass="11039" checksum="411B2041D3244FE1" modified="2023-09-13" version="1">MCHATLRCSVEEGGENVTYEWTSMGQGAVVSKKGSILNDSWSLGDLNWTYTCTAMNTVSNSVSNLIHAGQLCAVSKAATGTYCPVTWILLVKVLLLLFSWGF</sequence>
</entry>
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    <accession>A0A9L0J872</accession>
    <name>A0A9L0J872_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">C-type lectin domain containing 16A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">CLEC16A</name>
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    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
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                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
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                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
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                <person name="X."/>
                <person name="Yin"/>
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                <person name="Ji"/>
                <person name="C."/>
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                <person name="Yue"/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CLEC16A/gop-1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J872"/>
    <dbReference type="Ensembl" id="ENSEAST00005073591.1">
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        <property type="gene ID" value="ENSEASG00005011527.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 14"/>
    </dbReference>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901097">
        <property type="term" value="P:negative regulation of autophagosome maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039272">
        <property type="entry name" value="CLEC16A/TT9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045820">
        <property type="entry name" value="CLEC16A/TT9_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019155">
        <property type="entry name" value="CLEC16A/TT9_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21481">
        <property type="entry name" value="PROTEIN CLEC16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21481:SF0">
        <property type="entry name" value="PROTEIN CLEC16A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19439">
        <property type="entry name" value="CLEC16A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09758">
        <property type="entry name" value="FPL"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FPL" evidence="4">
        <location>
            <begin position="51"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="CLEC16A/TT9 C-terminal" evidence="5">
        <location>
            <begin position="245"/>
            <end position="1018"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="376"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="451"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="890"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1016"/>
            <end position="1051"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="381"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
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            <begin position="891"/>
            <end position="936"/>
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    </feature>
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            <begin position="1028"/>
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    </feature>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J882</accession>
    <name>A0A9L0J882_EQUAS</name>
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        <recommendedName>
            <fullName evidence="14">Small ribosomal subunit protein uS4</fullName>
        </recommendedName>
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        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">RPS9</name>
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    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
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    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Gao"/>
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                <person name="Ju"/>
                <person name="Z."/>
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                <person name="J."/>
                <person name="Li"/>
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                <person name="Hou"/>
                <person name="M."/>
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                <person name="Pan"/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014712052.1">
        <property type="nucleotide sequence ID" value="XM_014856566.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044614850.1">
        <property type="nucleotide sequence ID" value="XM_044758915.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J882"/>
    <dbReference type="SMR" id="A0A9L0J882"/>
    <dbReference type="Ensembl" id="ENSEAST00005060821.1">
        <property type="protein sequence ID" value="ENSEASP00005049506.1"/>
        <property type="gene ID" value="ENSEASG00005028556.1"/>
    </dbReference>
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    <dbReference type="KEGG" id="eai:106840884"/>
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    <dbReference type="GeneTree" id="ENSGT00550000074829"/>
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        <property type="component" value="Chromosome 26"/>
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        <property type="term" value="C:cytosolic small ribosomal subunit"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:rRNA binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:translation"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="40S ribosomal protein S9"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
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    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_uS4_CS"/>
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    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_uS4_euk/arc"/>
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    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003139">
        <property type="entry name" value="PRK04051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF46">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US4"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 18" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="10 18" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="9 17" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 17" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="21">
        <location>
            <begin position="3"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="RNA-binding S4" evidence="20">
        <location>
            <begin position="108"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="162"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
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    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00035254"/>
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        <source>
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            <dbReference type="RuleBase" id="RU003699"/>
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    <evidence type="ECO:0000256" key="19">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM00363"/>
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        <source>
            <dbReference type="SMART" id="SM01390"/>
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    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005049506.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="194" mass="22591" checksum="E9CE3CBD59524F81" modified="2023-09-13" version="1">MPVARSWVCRKTYVTPRRPFEKSRLDQELKLIGEYGLRNKREVWRVKFTLAKIRKAARELLTLDEKDPRRLFEGNALLRRLVRIGVLDEGKMKLDYILGLKIEDFLERRLQTQVFKLGLAKSIHHARVLIRQRHIRVRKQVVNIPSFIVRLDSQKHIDFSLRSPYGGGRPGRVKRKNAKKGQGGAGAGDDEEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JAM0</accession>
    <name>A0A9L0JAM0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070348203.1">
        <property type="nucleotide sequence ID" value="XM_070492102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JAM0"/>
    <dbReference type="Ensembl" id="ENSEAST00005077970.1">
        <property type="protein sequence ID" value="ENSEASP00005050356.1"/>
        <property type="gene ID" value="ENSEASG00005024885.1"/>
    </dbReference>
    <dbReference type="GeneID" id="139041296"/>
    <dbReference type="GeneTree" id="ENSGT00940000153505"/>
    <dbReference type="OMA" id="HECNESG"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:004935">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002402">
        <property type="entry name" value="Zinc finger protein 347"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000023">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000060">
        <property type="entry name" value="zinc finger protein 436"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000011">
        <property type="entry name" value="zinc finger protein 615 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000307">
        <property type="entry name" value="Zinc finger protein ZFP69 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050589">
        <property type="entry name" value="Ikaros_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404:SF114">
        <property type="entry name" value="KLUMPFUSS, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24404">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="228"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="256"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="284"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="312"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="340"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="368"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="396"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="424"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="452"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JEE2</accession>
    <name>A0A9L0JEE2_EQUAS</name>
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            <fullName evidence="13 14">Multifunctional fusion protein</fullName>
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        </domain>
    </protein>
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        <name evidence="16" type="primary">ALDH4A1</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 13">
            <text>L-glutamate 5-semialdehyde + NAD(+) + H2O = L-glutamate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:30235"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="EC" id="1.2.1.88"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.88" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0JEE2"/>
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        <property type="gene ID" value="ENSEASG00005015650.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00560000077335"/>
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        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003842">
        <property type="term" value="F:L-glutamate gamma-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07123">
        <property type="entry name" value="ALDH_F4-17_P5CDH"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="1-pyrroline-5-carboxylate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000005">
        <property type="entry name" value="1-pyrroline-5-carboxylate dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005931">
        <property type="entry name" value="P5CDH/ALDH4A1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01236">
        <property type="entry name" value="D1pyr5carbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14516">
        <property type="entry name" value="1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14516:SF3">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 13" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="15">
        <location>
            <begin position="168"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
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    <accession>A0A9L0JI08</accession>
    <name>A0A9L0JI08_EQUAS</name>
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        <recommendedName>
            <fullName evidence="4">steroid 11beta-monooxygenase</fullName>
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        </recommendedName>
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            <fullName evidence="14">Cytochrome P450C11</fullName>
        </alternativeName>
    </protein>
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        <name evidence="19" type="primary">LOC106827552</name>
    </gene>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
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                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
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                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
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                <person name="J."/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>21-hydroxyprogesterone + 2 reduced [adrenodoxin] + O2 + 2 H(+) = corticosterone + 2 oxidized [adrenodoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:46104"/>
            <dbReference type="Rhea" id="RHEA-COMP:9998"/>
            <dbReference type="Rhea" id="RHEA-COMP:9999"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16827"/>
            <dbReference type="ChEBI" id="CHEBI:16973"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:46105"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a steroid + 2 reduced [adrenodoxin] + O2 + 2 H(+) = an 11beta-hydroxysteroid + 2 oxidized [adrenodoxin] + H2O</text>
            <dbReference type="Rhea" id="RHEA:15629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9998"/>
            <dbReference type="Rhea" id="RHEA-COMP:9999"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:35341"/>
            <dbReference type="ChEBI" id="CHEBI:35346"/>
            <dbReference type="EC" id="1.14.15.4"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:15630"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.15.4" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0JI08"/>
    <dbReference type="Ensembl" id="ENSEAST00005064147.1">
        <property type="protein sequence ID" value="ENSEASP00005049637.1"/>
        <property type="gene ID" value="ENSEASG00005036856.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163354"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047783">
        <property type="term" value="F:corticosterone 18-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004507">
        <property type="term" value="F:steroid 11-beta-monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032342">
        <property type="term" value="P:aldosterone biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071375">
        <property type="term" value="P:cellular response to peptide hormone stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008203">
        <property type="term" value="P:cholesterol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034650">
        <property type="term" value="P:cortisol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006704">
        <property type="term" value="P:glucocorticoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000015">
        <property type="entry name" value="Cholesterol side-chain cleavage enzyme, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050479">
        <property type="entry name" value="CYP11_CYP27_families"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24279:SF1">
        <property type="entry name" value="CYTOCHROME P450 11B2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 17 18" id="KW-0349">Heme</keyword>
    <keyword evidence="9 17 18" id="KW-0408">Iron</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 17 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10 18" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="8 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0755">Steroidogenesis</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="binding site" description="axial binding residue" evidence="17">
        <location>
            <position position="452"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00042800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005049637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="505" mass="57864" checksum="EE19B7D0E5B01A47" modified="2023-09-13" version="1">MAFRAKADVRLAGSWLSLRWAHSLGSRATPAPKAMLPFEAMPECPGNRWMRVLQIWREQGSENLHLEIHQTFQELGPIFRYHMGGTQTVFVMLPEDVKRLQQVESHHPRRATVGPWLAYRQHCGHKYGVFLLNGPEWRYDRLRLNPDVLSPQAVEKYIPMVDGVVARDFSKALKSKVLLQNARGSLTLDARPSIFHYTIEASNLALFGERLGLLGHSPSPASLHFIRALETMFKSTVQLMFMPRSLSRWTSTKAWKEHFEAWDYIFQYANNSIQKIYQELALGHPRHYSGIVAELLLRADMSLDAIRANSIDLTAGSVDTTAYPLLMTLFELARNPKVQQALRQESLVAEARISENPRRATTELPLLRAALKETLRLYPVGVFLEREVSSDLVLQNYHIPAGTTVKVALYSLGRNPAVFVRPERYHPQRWLDNRASSTRFPYLAFGFGLRQCLGRRLAEVEMLLLLHHVLKNFVVETLTQEDVKMIYRFILMPSTLPLLTFRAIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JI19</accession>
    <name>A0A9L0JI19_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Uteroglobin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Secretoglobin family 1A member 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">LOC123278007</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the secretoglobin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JI19"/>
    <dbReference type="Ensembl" id="ENSEAST00005060315.1">
        <property type="protein sequence ID" value="ENSEASP00005052844.1"/>
        <property type="gene ID" value="ENSEASG00005031748.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155073"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019834">
        <property type="term" value="F:phospholipase A2 inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00633">
        <property type="entry name" value="Secretoglobin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.210.10:FF:000001">
        <property type="entry name" value="Uteroglobin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.210.10">
        <property type="entry name" value="Secretoglobin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016126">
        <property type="entry name" value="Secretoglobin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043215">
        <property type="entry name" value="Secretoglobin_1C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035960">
        <property type="entry name" value="Secretoglobin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000329">
        <property type="entry name" value="Uteroglobin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10136">
        <property type="entry name" value="SECRETOGLOBIN FAMILY 1 MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10136:SF6">
        <property type="entry name" value="UTEROGLOBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01099">
        <property type="entry name" value="Uteroglobin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00486">
        <property type="entry name" value="UTEROGLOBIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00096">
        <property type="entry name" value="UTG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48201">
        <property type="entry name" value="Uteroglobin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51311">
        <property type="entry name" value="SCGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0593">Phospholipase A2 inhibitor</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="122" mass="13045" checksum="3ED26D4F2B72A6E7" modified="2023-09-13" version="1">TISYPSPSTKRLLSLADRPGCLHLGWAQPGSWPAWKGAWIHVLSLSRAPASAEICQSFAGVIQSLFLGTPASFEAAVEPFNPDADMKAAATQLKTLVDLLPKNTKDSILKLMDKIAKSPLCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JK10</accession>
    <name>A0A9L0JK10_EQUAS</name>
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        <recommendedName>
            <fullName evidence="17 20">Adenosine kinase</fullName>
            <shortName evidence="20">AK</shortName>
            <ecNumber evidence="5 20">2.7.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18 20">Adenosine 5'-phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">ADK</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives.</text>
    </comment>
    <comment type="function">
        <text evidence="16">Catalyzes the phosphorylation of the purine nucleoside adenosine at the 5' position in an ATP-dependent manner. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>adenosine + ATP = AMP + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20824"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.20"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:20825"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="20">Binds 3 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 20">Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 20">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.20" evidence="5 20"/>
    <dbReference type="RefSeq" id="XP_014716117.1">
        <property type="nucleotide sequence ID" value="XM_014860631.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JK10"/>
    <dbReference type="Ensembl" id="ENSEAST00005061014.1">
        <property type="protein sequence ID" value="ENSEASP00005052718.1"/>
        <property type="gene ID" value="ENSEASG00005022986.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106843549"/>
    <dbReference type="KEGG" id="eai:106843549"/>
    <dbReference type="CTD" id="132"/>
    <dbReference type="GeneTree" id="ENSGT00390000014320"/>
    <dbReference type="OrthoDB" id="432447at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004001">
        <property type="term" value="F:adenosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004136">
        <property type="term" value="F:deoxyadenosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044209">
        <property type="term" value="P:AMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106383">
        <property type="term" value="P:dAMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006175">
        <property type="term" value="P:dATP biosynthetic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032263">
        <property type="term" value="P:GMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01168">
        <property type="entry name" value="adenosine_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000175">
        <property type="entry name" value="Adenosine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001805">
        <property type="entry name" value="Adenokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45769:SF3">
        <property type="entry name" value="ADENOSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00989">
        <property type="entry name" value="ADENOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="13 20" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9 20" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 20" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="21">
        <location>
            <begin position="45"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="19">
        <location>
            <position position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00056297"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00068771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00080816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR601805-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU368116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="362" mass="40661" checksum="63D96B86840BBD93" modified="2023-09-13" version="1">MAAAEEERKPKKLKAEAPRALRENILFGMGNPLLDISAVVDKDFLDKYSLKANDQILAEDKHKELFDELVKKFKVEYHAGGSTQNSIKVAQWMIQQPHKAATFFGCIGIDKFGEILKKKTAEVHVDAHYYEQNEQPTGTCAVCITDDNRSLVANLAAANCYKKEKHLDMEKNWMLVEKARVYYIAGFFLTVSPESVLKVACHASENNRIFTLNLSAPFISQFFKESLMKVMPYVDILFGNETEAATFAREQGFETEDIKEIARKTQALPKVNSKRQRIVIFTQGRDDTIMATGSEVMAFPVLDQNQKEIVDTNGAGDAFVGGFLSQLVSDKPLTECIRAGHYAASVIIRRTGCTFPEKPDFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JL32</accession>
    <name>A0A9L0JL32_EQUAS</name>
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        <recommendedName>
            <fullName evidence="4">Protein kish-B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Transmembrane protein 167B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">TMEM167B</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the early part of the secretory pathway.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the KISH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JL32"/>
    <dbReference type="Ensembl" id="ENSEAST00005045782.1">
        <property type="protein sequence ID" value="ENSEASP00005053939.1"/>
        <property type="gene ID" value="ENSEASG00005030330.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162196"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042863">
        <property type="entry name" value="Kish-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009653">
        <property type="entry name" value="Ksh1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46815">
        <property type="entry name" value="PROTEIN KISH-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46815:SF1">
        <property type="entry name" value="PROTEIN KISH-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06842">
        <property type="entry name" value="DUF1242"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005053939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="119" mass="12947" checksum="8E7725BC54F0C37D" modified="2023-09-13" version="1">MTNVYSLDGILVFGLLFVCTCAYFKKVPRLKTWLLSEKKGVWGVFYKGKIISGQGEEIAISGQGATNRCDWNQAACCCGNRLHCNGLLRLIYKMNSKASISPTANQGDEGEEPVGLGPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JMF7</accession>
    <name>A0A9L0JMF7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">G-patch domain and KOW motifs-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">GPKOW</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">RNA-binding protein involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the MOS2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JMF7"/>
    <dbReference type="Ensembl" id="ENSEAST00005047856.1">
        <property type="protein sequence ID" value="ENSEASP00005051162.1"/>
        <property type="gene ID" value="ENSEASG00005019852.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015154"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13152">
        <property type="entry name" value="KOW_GPKOW_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13153">
        <property type="entry name" value="KOW_GPKOW_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041993">
        <property type="entry name" value="GPKOW_KOW1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041994">
        <property type="entry name" value="GPKOW_KOW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045166">
        <property type="entry name" value="Spp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026822">
        <property type="entry name" value="Spp2/MOS2_G-patch"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15818">
        <property type="entry name" value="G PATCH AND KOW-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15818:SF2">
        <property type="entry name" value="G-PATCH DOMAIN AND KOW MOTIFS-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12656">
        <property type="entry name" value="G-patch_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25088">
        <property type="entry name" value="GPKOW_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="G-patch" evidence="7">
        <location>
            <begin position="164"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="216"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="299"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="224"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="324"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005051162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="484" mass="53262" checksum="D6BDACDA551A4701" modified="2023-09-13" version="1">MADAEEGVLRPAGASTAPISFGFSRTSARRRLADPGDGAGAPPEEKDFLKTVEGRELQSVKPSEAPKELVIPLIQNGHRRQPLTQAPGPSTDTEALADGMLSQAVKELIEESKKSLEERENAGVDPTLAIPMIQKRCTPSGEGADSERWAETVPEEADYEAVPVEAYGLALLRGMGWKPGEGIGRTFNQVVKPRVNSLRPKGLGLGANLTEVQALAPTGSHRLPRPDEEQEKDKEDQPQGLVPGGAVVVRSGPHRGLYGKVEGLDPDNVRAMVRLAVGSRVVTLSEYYLQPVSQQEFDKNSMELSQRNRSSPGQQNGTASSWKALRDQDLHIRQEDSERKRKHLPDRQDGPAAKSEKAAPRSQHWLHRDLRVRFVDKLHKGGQYYNTKMTIEDVLSPSTCVCRTDEGQVLEGLREDMLETLVPKVEGNRVMVVLGPQAGRVGRLLGRDGERSRALVQLRRENRLVELHYDAVCQYVGPSDSEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JMS9</accession>
    <name>A0A9L0JMS9_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Solute carrier family 35 member G6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SLC35G6</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the SLC35G solute transporter family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JMS9"/>
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        <name type="scientific">Equus asinus</name>
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        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Metazoa</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="ZINC FINGER MYM-TYPE PROTEIN"/>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
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        </location>
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            <begin position="26"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JR77</accession>
    <name>A0A9L0JR77_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Citrate synthase</fullName>
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    </protein>
    <gene>
        <name evidence="11" type="primary">CS</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Zhao"/>
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                <person name="Zhang"/>
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                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Zhuang"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
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                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
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                <person name="Liu"/>
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                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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                <person name="Li"/>
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                <person name="Li"/>
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                <person name="Liu"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_014841556.3"/>
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        <location>
            <begin position="1"/>
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        <source>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Li"/>
                <person name="S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <property type="entry name" value="SAM_domain-containing_protein"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="118"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005055979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="202" mass="22744" checksum="6B6414CCC6367E1F" modified="2023-09-13" version="1">MFTELRTKLSPPRGRAGAVRAGFGERRDVDATAHFSFCRTLLEHTVSAESIPCHLPRTPGTSLTWHDSRSQRAAGDRPVKLLQQPGTEAPQGRLYAEHYGLYHTSPSLGGLTRPVVLWSQQDVCKWLKKHCPHNYLVYVEAFSQHAITGRALLRLNAEKLQRMGLAQEAQRQEVLQQVLRLQLPSETCRSCCLRLEPQTPAL</sequence>
</entry>
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    <accession>A0A9L0JS52</accession>
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        <submittedName>
            <fullName evidence="9">TUB like protein 3</fullName>
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    </protein>
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        <name evidence="9" type="primary">TULP3</name>
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    <reference evidence="9 10" key="1">
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TUB family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A9L0JS52"/>
    <dbReference type="Ensembl" id="ENSEAST00005041599.1">
        <property type="protein sequence ID" value="ENSEASP00005056141.1"/>
        <property type="gene ID" value="ENSEASG00005017122.2"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
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    <dbReference type="GO" id="GO:0005929">
        <property type="term" value="C:cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005737">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0061512">
        <property type="term" value="P:protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="FunFam" id="3.20.90.10:FF:000001">
        <property type="entry name" value="Tubby-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.90.10">
        <property type="entry name" value="Tubby Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tubby-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000007">
        <property type="entry name" value="Tubby_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018066">
        <property type="entry name" value="Tubby_C_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005398">
        <property type="entry name" value="Tubby_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517">
        <property type="entry name" value="TUBBY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16517:SF138">
        <property type="entry name" value="TUBBY-RELATED PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01167">
        <property type="entry name" value="Tub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16322">
        <property type="entry name" value="Tub_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01573">
        <property type="entry name" value="SUPERTUBBY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01574">
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Zhao"/>
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                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Liu"/>
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                <person name="Liu"/>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JV44</accession>
    <name>A0A9L0JV44_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BLOC-1-related complex subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">BORCS5</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BORCS5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014689688.1">
        <property type="nucleotide sequence ID" value="XM_014834202.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JV44"/>
    <dbReference type="Ensembl" id="ENSEAST00005078381.1">
        <property type="protein sequence ID" value="ENSEASP00005057029.1"/>
        <property type="gene ID" value="ENSEASG00005029117.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106826718"/>
    <dbReference type="KEGG" id="eai:106826718"/>
    <dbReference type="CTD" id="118426"/>
    <dbReference type="GeneTree" id="ENSGT00390000015016"/>
    <dbReference type="OMA" id="EGRPHDP"/>
    <dbReference type="OrthoDB" id="10035640at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099078">
        <property type="term" value="C:BORC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098574">
        <property type="term" value="C:cytoplasmic side of lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032418">
        <property type="term" value="P:lysosome localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072384">
        <property type="term" value="P:organelle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903744">
        <property type="term" value="P:positive regulation of anterograde synaptic vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22789">
        <property type="entry name" value="BORCS5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018780">
        <property type="entry name" value="TBORCS5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31634">
        <property type="entry name" value="BLOC-1-RELATED COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31634:SF2">
        <property type="entry name" value="BLOC-1-RELATED COMPLEX SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10158">
        <property type="entry name" value="LOH1CR12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0458">Lysosome</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005057029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="196" mass="22192" checksum="CC354D58616F8EB8" modified="2023-09-13" version="1">MGSEQSSEAESRPNDLNSSVTPSPAKHRAKMDDIVVVAQGSQASRNVSNDPDVIKLQEIPTFQPLLKGLLSGQTSPTNAKLEKLDSQQVLQLCLRYQDHLHQCAEAVAFDQNALVKRIKEMDLSVETLFSFMQERQKRYAKYAEQIQKVNEMSAILRRIQMGIDQTVPLMERLNSLLPEGERLEPFSMKPDREPQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JVA6</accession>
    <name>A0A9L0JVA6_EQUAS</name>
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        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106823674</name>
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    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014684978.1">
        <property type="nucleotide sequence ID" value="XM_014829492.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JVA6"/>
    <dbReference type="Ensembl" id="ENSEAST00005075241.1">
        <property type="protein sequence ID" value="ENSEASP00005056398.1"/>
        <property type="gene ID" value="ENSEASG00005038042.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106823674"/>
    <dbReference type="KEGG" id="eai:106823674"/>
    <dbReference type="GeneTree" id="ENSGT00940000162532"/>
    <dbReference type="OrthoDB" id="9444602at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15234">
        <property type="entry name" value="7tmA_OR7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000009">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48001">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="103"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="237"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-methionyl-[F-actin] + NADPH + O2 + H(+) = L-methionyl-(R)-S-oxide-[F-actin] + NADP(+) + H2O</text>
            <dbReference type="Rhea" id="RHEA:51308"/>
            <dbReference type="Rhea" id="RHEA-COMP:12953"/>
            <dbReference type="Rhea" id="RHEA-COMP:12956"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.14.13.225"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the Mical family.</text>
    </comment>
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    <dbReference type="Ensembl" id="ENSEAST00005067797.1">
        <property type="protein sequence ID" value="ENSEASP00005059096.1"/>
        <property type="gene ID" value="ENSEASG00005005893.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158780"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120501">
        <property type="term" value="F:F-actin monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd21195">
        <property type="entry name" value="CH_MICAL2_3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09439">
        <property type="entry name" value="LIM_Mical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000004">
        <property type="entry name" value="protein-methionine sulfoxide oxidase MICAL2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.418.10:FF:000026">
        <property type="entry name" value="protein-methionine sulfoxide oxidase MICAL3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000043">
        <property type="entry name" value="protein-methionine sulfoxide oxidase MICAL3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.418.10">
        <property type="entry name" value="Calponin-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022735">
        <property type="entry name" value="bMERB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001715">
        <property type="entry name" value="CH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036872">
        <property type="entry name" value="CH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050540">
        <property type="entry name" value="F-actin_Monoox_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
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    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057494">
        <property type="entry name" value="Rossman_Mical"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167:SF39">
        <property type="entry name" value="[F-ACTIN]-MONOOXYGENASE MICAL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23167">
        <property type="entry name" value="CALPONIN HOMOLOGY DOMAIN-CONTAINING PROTEIN DDB_G0272472-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12130">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00307">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF25413">
        <property type="entry name" value="Rossman_Mical"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00033">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01203">
        <property type="entry name" value="DUF3585"/>
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    </dbReference>
    <dbReference type="SMART" id="SM00132">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47576">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50021">
        <property type="entry name" value="CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="19" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14 18" id="KW-0440">LIM domain</keyword>
    <keyword evidence="8 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 18" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Calponin-homology (CH)" evidence="21">
        <location>
            <begin position="516"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="22">
        <location>
            <begin position="1000"/>
            <end position="1062"/>
        </location>
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            <begin position="1799"/>
            <end position="1948"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="660"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="747"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="887"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1063"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1166"/>
            <end position="1328"/>
        </location>
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        <location>
            <begin position="1364"/>
            <end position="1636"/>
        </location>
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        <location>
            <begin position="1689"/>
            <end position="1796"/>
        </location>
    </feature>
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        <location>
            <begin position="1874"/>
            <end position="1942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="664"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="765"/>
            <end position="774"/>
        </location>
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    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="896"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="20">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
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            <begin position="1185"/>
            <end position="1196"/>
        </location>
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            <begin position="1239"/>
            <end position="1250"/>
        </location>
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            <begin position="1257"/>
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        </location>
    </feature>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K2D8</accession>
    <name>A0A9L0K2D8_EQUAS</name>
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        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106821889</name>
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    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014682247.3">
        <property type="nucleotide sequence ID" value="XM_014826761.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K2D8"/>
    <dbReference type="Ensembl" id="ENSEAST00005044793.1">
        <property type="protein sequence ID" value="ENSEASP00005059671.1"/>
        <property type="gene ID" value="ENSEASG00005028945.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106821889"/>
    <dbReference type="KEGG" id="eai:106821889"/>
    <dbReference type="GeneTree" id="ENSGT00950000183216"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="CDD" id="cd15234">
        <property type="entry name" value="7tmA_OR7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000009">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48001">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
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    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="23"/>
            <end position="47"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="59"/>
            <end position="78"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
            <end position="120"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="140"/>
            <end position="158"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="237"/>
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    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="290"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005059671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
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                <person name="Li"/>
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                <person name="Huang"/>
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                <person name="Min"/>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
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        <property type="protein sequence ID" value="ENSEASP00005056547.1"/>
        <property type="gene ID" value="ENSEASG00005033101.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000161727"/>
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        <property type="component" value="Chromosome 13"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Asialoglycoprotein receptor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="C-type_lectin-like"/>
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        <property type="entry name" value="C-type_lectin-like/link_sf"/>
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        <property type="entry name" value="C-type_lectin/snaclec_domain"/>
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        <property type="entry name" value="C-type_lectin_CS"/>
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    <dbReference type="InterPro" id="IPR033989">
        <property type="entry name" value="CD209-like_CTLD"/>
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    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
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    <dbReference type="PANTHER" id="PTHR22803">
        <property type="entry name" value="MANNOSE, PHOSPHOLIPASE, LECTIN RECEPTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03954">
        <property type="entry name" value="Lectin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0430">Lectin</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="C-type lectin" evidence="9">
        <location>
            <begin position="312"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="19"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005056547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="436" mass="48208" checksum="2B184E1E429BE0D6" modified="2023-09-13" version="1">MKLLGDGSGQCPLMEEGATREERMKGTPPHPSSQGCISASGSNLRGLKSGYSAFSLCIQPKRSGIHGERKKTPSLAPLRGELTPQLAAHSMSLLRFTDWVLKALRRATSSPATLSTPLLWNPPPPALPGPQPPAAGGCLCDRIPEWVPRGWAGKGAMLRGTVVEGELVAVMVNAAPSFGSIPTGSCPDSKLQEELQALRKTFSNFTVSTEAEVKALSVQGEEGWGWGWEAAEGWRRWDTEQVSPPGGGVGRKMKSLESQLEKQQQELSEDHSSLLLHVKQFVSDLRSLSCQMAILQGNGSERTCCPVNWLEHEGSCYWFSRSGKPWPEANKYCQLENAHLVVVGSWDEQKFVQHHMGPVHTWMGLTDQNGPWKWVDGTDYETGFKNWRPEQPDDWYGHGLGGGEDCAHFTEDGRWNDDVCQRPYRWVCETELNRDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K6R1</accession>
    <name>A0A9L0K6R1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">FH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the formin homology family. Cappuccino subfamily.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005054900.1">
        <property type="protein sequence ID" value="ENSEASP00005060523.1"/>
        <property type="gene ID" value="ENSEASG00005003276.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161899"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005884">
        <property type="term" value="C:actin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045010">
        <property type="term" value="P:actin nucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030866">
        <property type="term" value="P:cortical actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2220:FF:000029">
        <property type="entry name" value="Formin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.2220:FF:000030">
        <property type="entry name" value="Formin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015425">
        <property type="entry name" value="FH2_Formin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001265">
        <property type="entry name" value="Formin_Cappuccino_subfam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920">
        <property type="entry name" value="FORMIN HOMOLOGY 2 DOMAIN CONTAINING, ISOFORM I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45920:SF7">
        <property type="entry name" value="FORMIN-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02181">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00828">
        <property type="entry name" value="FORMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00498">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101447">
        <property type="entry name" value="Formin homology 2 domain (FH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51444">
        <property type="entry name" value="FH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FH2" evidence="3">
        <location>
            <begin position="1227"/>
            <end position="1621"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="206"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="404"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="600"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="777"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="845"/>
            <end position="1200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1333"/>
            <end position="1352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="61"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="159"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="173"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="206"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="270"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="327"/>
            <end position="338"/>
        </location>
    </feature>
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            <begin position="371"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="409"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="431"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="793"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="858"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="870"/>
            <end position="1191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005060523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1645" mass="172112" checksum="29C32F400B584C34" modified="2023-09-13" version="1">MGNQDGKLKRSAGDALHEGGGGAEDAVGPRDVEATKKGSGGKKALGKHGKGGGGGGGGGEPGKKKSKSDSRASVFSNLRIRKNLSKGKGAGGSREDVLDSQALQTGELDSAHSLATKTPDLSLSADETGLSDTECADPFEVTRPGGPGPAEAGAGGRAVAGDLEAAAGAQDGQRTSSGSDTDIYSFHSATEQEDLLSDIQQAIRLQLQLQGSEEPAAPPTAASPQTGAFLGLDQFLLGPSSAAGEAPGSPDTEQALSALSDLPESLAAEPPLPEQPQPPGAHGASEAPGLPAAQPATADSPSSTAFPFPESVPGEGAAGAPVRGAGDTDEEGEEDAFEDAPRGSPGEEWGLERGEAPQRLGAEPEEGARGPGAPAAASLPGSPAPSPRCFKPYPLITPCYIKTTTRQLSSPNHSPNQSPSQSPRIKRRLESSLSRGPRAALASAAAPAKKQRADGGLAGGLSRSADWTEELGARAPRPGGSAHLLERAAGPPGGGGAPPARAQPPAGGFQNVFTGRTLLEKLFSQQENGPPEEAEKFCSRIIAMGLLLPFSDCFREPCRQNAPSSSAPFDQDQLYTWAAVSQPTHSLDCTEGQFPRRVPSVWPPSGPPDEEHGPTAADAECQSAVLETPRKSSDAVQQEVFDMRSEGQATVIQQLEQTIEDLRTKIAELEKHYPTVDMEMTSGHHGIQNGLAPSEDVCLEALRLGEKDVQHQRILQAKSIQTSPTEEGGIPTLPSVDALPASPPCPPGAEHGPQTKFCSEISLIVSPRRISLQLDTHQSIRPSPPPSLPCSDGQGQAGSQPSHPSLHTEFETSHEHYVFSSFENGCNIPPPPPLPCTESSSSLPGLGMAVAPPPPLPGMTGPALPSAAIPSPPPLPSTEMLPPPPPPLPGEEITPPPPLPGMGIPSPPPLPGMGIPSPPPLPGMGIPPPPPLPGMGIPSPPPLPGMGIPSPPPLPGMGIPPPPPPPLPGMGIPSPPPLPGMGIPSPPPLPGMGIPPPPPLPGMGIPSPPPLPGMGIPPPPPLPGMGIPPPPPLPGMGIPPPPTLPGVGIPPTPPLPGGGIPPPPPLPGVGIPPPPPLPGVGIPPPPPLPGVGIPPPPPLPGVGIPPPPPLPGLGIPPPPPLPGVGIPPPPPLPGVGIPPPPPLPGVGIPPPPPLPGMGIPPAPAPPPHPGTGIPPPPPPPLLPGSGLPPAPQAGGSTLPTPQVCGFLPPPLPTGLFGLGMNQDKGSRKRPIEPCRPMKPLYWTRIQLQSRRDSSPSLIWEKIEEPSIDCHEFEELFSKTAVKERKKPISDTITKTKAKQVVKLLSNKRSQAVGILMSSLHLDMKDIQHRAQSDELEKIEKHSRSSKDKENAKSLDKPEQFLYELSLIPNFSERVFCILFQSTFSESICSIRRKLELLQKLCETLANGAGVTQVLGLVLAFGNYMNGGNKTRGQADGFGLDILPKLKDVKSSDNSRSLLSYIVSYYLRNFDEDAGKEQCVFPLPEPQDLFQASQMKFEDFQKDLRKLKKDLKACEIEAGKVYQASSKEHIQPFKENMEQFIIQAKIDQEAEENSLTETHKCFLETTAYFFMKPKIGEKEVSPNVFFSIWHEFSSDFKDFWKKENKLILQERVREAEEVCRQKKGKSLYKIKPRHDSGIKAKISMKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K8R4</accession>
    <name>A0A9L0K8R4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC123281259</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K8R4"/>
    <dbReference type="Ensembl" id="ENSEAST00005048052.1">
        <property type="protein sequence ID" value="ENSEASP00005057705.1"/>
        <property type="gene ID" value="ENSEASG00005024047.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154693"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001833">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000348">
        <property type="entry name" value="zinc finger protein 260"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000135">
        <property type="entry name" value="Zinc finger protein 358"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000200">
        <property type="entry name" value="zinc finger protein 510 isoform X2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000292">
        <property type="entry name" value="zinc finger protein 619"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050758">
        <property type="entry name" value="Znf_C2H2-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234:SF10">
        <property type="entry name" value="RIKEN CDNA 6720489N17 GENE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="14">
        <location>
            <begin position="14"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="230"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="258"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="286"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="314"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="342"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="370"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="398"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="426"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="454"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="482"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="510"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="538"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="566"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005057705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="590" mass="67484" checksum="114E60BE991448B8" modified="2023-09-13" version="1">MAAAARREPAQGCVTFEDVAIYFSQEEWGLLDEAQRILYHDVMLENFVLTTSFDCWCGVEDEEVVSDQRISVEGVSQVRTSKAGPSTQSTQLCEKCIPVLEDVLHLPELQATYPGQKPYLDGASRGFWFSTNLQQYQKHNSRGKIFKMDMDQPSFVTSSRFHVLGKPFTYGESGKDFLATSGLLQQQAIPGCGEPFNSGNSHDKWLECRKVFSNTDTLLRQRVSTGKGLDECNKFGKSFNCKYKLGQHQQIHTGERPYECTECRKSFTYKSHLIRHQRVHTGVRPYECNECGKSFSQKSILIQHQRVHTGERPYECSGCGKFFSQKSALVHHERVHTGERPYECSECGKTFTFSSRLRYHQRVHTGERPYECSECGKAFTFSFRLRDHQRVHTGGGPYECIECGKSFSANSVLIQHQRVHTGERPYECSECGKSFSQSSGLLLHKRAHTRARLYECTDCGKAFGCKSKLVQHQRIHTGVRPYECIECGKSFSVKFVLIQHQRIHSGERPYECSQCGKSFSQKSVLIQHQRIHTGERPYDCSECWKSFSRKAQLIQHRTVHSGARPHECSECGKSFSQRSGLLRHRRAHVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KB75</accession>
    <name>A0A9L0KB75_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AIG1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. IAN subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KB75"/>
    <dbReference type="Ensembl" id="ENSEAST00005071862.1">
        <property type="protein sequence ID" value="ENSEASP00005058520.1"/>
        <property type="gene ID" value="ENSEASG00005038527.1"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006703">
        <property type="entry name" value="G_AIG1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04548">
        <property type="entry name" value="AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AIG1-type G" evidence="3">
        <location>
            <begin position="53"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005058520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="104" mass="11580" checksum="383F6B01E7AF226D" modified="2023-09-13" version="1">MLVGNSGGCPWVGILGEKQLEVHHNTATFVPYLQLRLVLVGKTGAGKSSTGNKLMKEFGDRYCLFNNKATGAEQEAQRAQLLALVEHVDELESSRRKWHKWRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KCB1</accession>
    <name>A0A9L0KCB1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Leukotriene B4 receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LTB4R</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
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    <comment type="similarity">
        <text evidence="11">Belongs to the chemokine-like receptor (CMKLR) family.</text>
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        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Sun"/>
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                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
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                <person name="Zhuang"/>
                <person name="G."/>
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                <person name="Q."/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
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                <person name="Yin"/>
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                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
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                <person name="Yue"/>
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                <person name="Liu"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <property type="entry name" value="Znf_C2H2_sf"/>
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        <property type="entry name" value="U11_U12 SMALL NUCLEAR RIBONUCLEOPROTEIN 48 KDA PROTEIN"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
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    <feature type="compositionally biased region" description="Basic residues" evidence="5">
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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        <source>
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        </source>
    </evidence>
    <sequence length="339" mass="39855" checksum="BE44227593B2BF98" modified="2023-09-13" version="1">MEVEPPPVEERRRLQEDLSEFVESCCRTLEEVTASLGWSLDRLDPGEAEAAEDEVVICPYDSNHHMPKSSLAKHMVSCQLRKLGYTKEEEDKMYNSDFFYENVKIPSITLNKDSQFQIIKQARTAVGKDGDCYNQRTYSSLPVEVPLNHKRCVCDLTQADRLALYDFVIEETKKKRSDSQIIENDSDLFVDLAAKVNQDNSRKSPKSYLEILAEVRDYKRRRQSYRAKNVHITKKSYTEVIRDVINVHMEELSSQWQEEQEKAEDAAEKNEERRSASVDSRQSGGSYLDAESSRHRRDRSRSPHKRKRNKDKDKNWDSRRRKERDGERHHSHRRRKQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KG52</accession>
    <name>A0A9L0KG52_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Claudin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106830069</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell junction</location>
            <location evidence="8">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the claudin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KG52"/>
    <dbReference type="Ensembl" id="ENSEAST00005042716.1">
        <property type="protein sequence ID" value="ENSEASP00005043642.1"/>
        <property type="gene ID" value="ENSEASG00005035036.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005079812.1">
        <property type="protein sequence ID" value="ENSEASP00005061622.1"/>
        <property type="gene ID" value="ENSEASG00005035036.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005080014.1">
        <property type="protein sequence ID" value="ENSEASP00005061089.1"/>
        <property type="gene ID" value="ENSEASG00005035036.1"/>
    </dbReference>
    <dbReference type="KEGG" id="eai:106830068"/>
    <dbReference type="GeneTree" id="ENSGT00940000161922"/>
    <dbReference type="OrthoDB" id="8612291at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000001">
        <property type="entry name" value="Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006187">
        <property type="entry name" value="Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017974">
        <property type="entry name" value="Claudin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12002">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01077">
        <property type="entry name" value="CLAUDIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01346">
        <property type="entry name" value="CLAUDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0796">Tight junction</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="82"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="118"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="165"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU060637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="220" mass="24457" checksum="8D57EEF7B6E55489" modified="2023-09-13" version="1">MAVVVRTVAQLAGLSLSLLGWVLSCLTNYLPQWKNLNLDLNEMENWTMGLWQTCVIQEEVGMQCKDFDSFLALPAELRGSRILMFLSNGLGLLGLLVSGFGLDCLRIGERQPACKKRLLILGGLLFWTAGITTVVPVSWVAHLIVREFWDETIPEIVPRWEFGEALFLGWFAGFSLLLGGCLLHCAACSTQAPPALGHHAVAEMQYPHQHLEMKNAHLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KIW5</accession>
    <name>A0A9L0KIW5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Barrier-to-autointegration factor-like protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Barrier-to-autointegration factor 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">BANF2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">May play a role in BANF1 regulation and influence tissue-specific roles of BANF1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Heterodimerizes with BANF1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KIW5"/>
    <dbReference type="Ensembl" id="ENSEAST00005062971.1">
        <property type="protein sequence ID" value="ENSEASP00005061605.1"/>
        <property type="gene ID" value="ENSEASG00005034425.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162003"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000793">
        <property type="term" value="C:condensed chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051276">
        <property type="term" value="P:chromosome organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.40:FF:000002">
        <property type="entry name" value="Barrier to autointegration factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.40">
        <property type="entry name" value="Barrier-to-autointegration factor, BAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051387">
        <property type="entry name" value="BAF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004122">
        <property type="entry name" value="BAF_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036617">
        <property type="entry name" value="BAF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47507">
        <property type="entry name" value="BARRIER TO AUTOINTEGRATION FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47507:SF4">
        <property type="entry name" value="BARRIER-TO-AUTOINTEGRATION FACTOR-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02961">
        <property type="entry name" value="SAM_BAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01023">
        <property type="entry name" value="BAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47798">
        <property type="entry name" value="Barrier-to-autointegration factor, BAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00059723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00065228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00074730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00079764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="97" mass="11156" checksum="D8E60645A0748A3B" modified="2023-09-13" version="1">GLRRTKKMDHMSPRLRAFLSEPIGEKDVVWVDGISHELAINLVTKGFNKAYTLLGQFLLMHKNEAEFQKWLICCFGATECEAQESSNCLKEWCTCFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KKN7</accession>
    <name>A0A9L0KKN7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2'-5'-oligoadenylate synthetase 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
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                <person name="Fang"/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
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                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Guo"/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Yin"/>
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                <person name="Ji"/>
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                <person name="Jin"/>
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                <person name="Yue"/>
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                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="Wu"/>
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                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the 2-5A synthase family.</text>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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                <person name="M."/>
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                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Belongs to the SNAPIN family.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="SNARE-ASSOCIATED PROTEIN SNAPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Snapin_Pallidin"/>
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    </dbReference>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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        <location>
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        <location>
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    </feature>
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        <location>
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    </feature>
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        <location>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DRAM/TMEM150 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014708303.1">
        <property type="nucleotide sequence ID" value="XM_014852817.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KUS4"/>
    <dbReference type="Ensembl" id="ENSEAST00005002090.2">
        <property type="protein sequence ID" value="ENSEASP00005001887.1"/>
        <property type="gene ID" value="ENSEASG00005001495.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106838598"/>
    <dbReference type="KEGG" id="eai:106838598"/>
    <dbReference type="CTD" id="129303"/>
    <dbReference type="GeneTree" id="ENSGT01030000234578"/>
    <dbReference type="OMA" id="DPAKHGY"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0072659">
        <property type="term" value="P:protein localization to plasma membrane"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="InterPro" id="IPR019402">
        <property type="entry name" value="CWH43_N"/>
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    <dbReference type="InterPro" id="IPR050911">
        <property type="entry name" value="DRAM/TMEM150_Autophagy_Mod"/>
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    <dbReference type="PANTHER" id="PTHR21324">
        <property type="entry name" value="FASTING-INDUCIBLE INTEGRAL MEMBRANE PROTEIN TM6P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21324:SF6">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 150A"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF10277">
        <property type="entry name" value="Frag1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="141"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="203"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="domain" description="CWH43-like N-terminal" evidence="11">
        <location>
            <begin position="4"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00030381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF10277"/>
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    </evidence>
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            <dbReference type="Ensembl" id="ENSEASP00005001887.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
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    <name>A0A8C4KV89_EQUAS</name>
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            <fullName evidence="10">Procollagen C-endopeptidase enhancer 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Procollagen COOH-terminal proteinase enhancer 2</fullName>
        </alternativeName>
    </protein>
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        <name evidence="16" type="primary">PCOLCE2</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Binds to the C-terminal propeptide of types I and II procollagens and may enhance the cleavage of that propeptide by BMP1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with heparin with high affinity, and type I or II collagen.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014707537.1">
        <property type="nucleotide sequence ID" value="XM_014852051.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KV89"/>
    <dbReference type="Ensembl" id="ENSEAST00005000273.2">
        <property type="protein sequence ID" value="ENSEASP00005000196.2"/>
        <property type="gene ID" value="ENSEASG00005000245.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106838129"/>
    <dbReference type="KEGG" id="eai:106838129"/>
    <dbReference type="CTD" id="26577"/>
    <dbReference type="GeneTree" id="ENSGT00940000157649"/>
    <dbReference type="OrthoDB" id="6116165at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008201">
        <property type="term" value="F:heparin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016504">
        <property type="term" value="F:peptidase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990830">
        <property type="term" value="P:cellular response to leukemia inhibitory factor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd03576">
        <property type="entry name" value="NTR_PCOLCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000005">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.120:FF:000015">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.290:FF:000026">
        <property type="entry name" value="Procollagen C-endopeptidase enhancer 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001134">
        <property type="entry name" value="Netrin_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018933">
        <property type="entry name" value="Netrin_module_non-TIMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035814">
        <property type="entry name" value="NTR_PCOLCE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008993">
        <property type="entry name" value="TIMP-like_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251">
        <property type="entry name" value="OVOCHYMASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24251:SF31">
        <property type="entry name" value="PROCOLLAGEN C-ENDOPEPTIDASE ENHANCER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01759">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00643">
        <property type="entry name" value="C345C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50242">
        <property type="entry name" value="TIMP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50189">
        <property type="entry name" value="NTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0358">Heparin-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040488464" description="Procollagen C-endopeptidase enhancer 2" evidence="13">
        <location>
            <begin position="24"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="14">
        <location>
            <begin position="33"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="14">
        <location>
            <begin position="154"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="domain" description="NTR" evidence="15">
        <location>
            <begin position="297"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="154"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00065159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000196.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="415" mass="45463" checksum="0F82DBC3494765D5" modified="2023-09-13" version="2">MRGAGAWAPLCLLLAAAAQPSRQQPPERPVFACGGILTGESGFIGSEGFPGVYPPNSKCTWKITVPEGKVVVLNFRFIDLESDNLCRYDFVDVYNGHANGQRIGRFCGTFRPGALVSSGNKMMVQMISDANTAGNGFMAMFSAAEPNERGDQYCGGLLERPSGSFTTPNWPDRDYPAGVTCVWHIVAPKNQLVELKFEKFDVERDNYCRYDYVAVFNGGEANDAQRIGKYCGDSPPAPIVSERNELLIQFLSDLSLTADGFIAHYQFRPKKLPTTTVPPVTTTFPVTTDLKPTVALCQQKCRRTGTLESNYCSSNFVLAGTVITTITRGGSLHATVSIINIYKEGNLAIQQAGKNMSAKVVVVCKQCPLLRRGLNYIIMGQVGEDGRGKIMPNSFIMMFKTKNQKLLNALENKQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KWT6</accession>
    <name>A0A8C4KWT6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Gametogenetin-binding protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Protein ZNF403</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">GGNBP2</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in spermatogenesis.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044601640.1">
        <property type="nucleotide sequence ID" value="XM_044745705.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070338947.1">
        <property type="nucleotide sequence ID" value="XM_070482846.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KWT6"/>
    <dbReference type="Ensembl" id="ENSEAST00005003032.2">
        <property type="protein sequence ID" value="ENSEASP00005002765.2"/>
        <property type="gene ID" value="ENSEASG00005002133.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106844686"/>
    <dbReference type="CTD" id="79893"/>
    <dbReference type="GeneTree" id="ENSGT00390000009552"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026073">
        <property type="entry name" value="GGNBP2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13601">
        <property type="entry name" value="GAMETOGENETIN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13601:SF2">
        <property type="entry name" value="GAMETOGENETIN-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="352"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00031743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005002765.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="696" mass="78978" checksum="841D79AE908FA91F" modified="2023-09-13" version="2">MARLVAVCRDGEEEFPFERRQIPLYIDDTLTMVMEFPDNVLNLDGHQNNGAQLKQFIQRHSMLKQQDLSIAMVVTSREVLSALSQLVPCVGCRRSVERLFSQLVESGNPALEPLTVGPKGVLSVTRSCMTDAKKLYTLFYVHGSKLNDMIDAIPKSKKNKRCQLHSLDTHKPKPLGGCWMDVWELMSQECRDEVVLIDSSCLLETLETYLRKHRFCTDCKNKVLRAYNILIGELDCSKEKGYCAALYEGLRCCPHERHIHVCCETDFIAHLLGRAEPEFAGGRRERHAKTIDIAQEEVLTCLGIHLYERLHRIWQKLRAEEQTWQMLFYLGVDALRKSFEMTVEKVQGISRLEQLCEEFSEEERVRELKQEKKRQKRKNRRKNKCVCDIPTPLQTADEKEVSQEKETDFIENSCKACGSTEDGNSCVEVIVTNENTSCTCPSSGSLLGSPKIKKGLSPHCNGSDCGYSSSMEGSETGSREGSDVACTEGICNHDEHVDDSCVHHCEDKEDDGDSCVECWANSEENTTKGKNKKKKKKSKMLKCDEHIQKLGSCITDPGNRETSGNTMHTVFHRDKTKDAHPESCCSSEKGGQPLPWFEHRKNVPQFAEPTETSFGPDSGKGAKSLVELLDESECTSDEEIFISQDEIQSFMANNQSFYSNREQYRQHLKEKFNKYCRLNDHKRPICSGWLTTAGAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L2A8</accession>
    <name>A0A8C4L2A8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Catenin delta 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CTNND1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Adherens junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the beta-catenin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014683306.1">
        <property type="nucleotide sequence ID" value="XM_014827820.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L2A8"/>
    <dbReference type="Ensembl" id="ENSEAST00005002778.2">
        <property type="protein sequence ID" value="ENSEASP00005002527.2"/>
        <property type="gene ID" value="ENSEASG00005001961.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822567"/>
    <dbReference type="CTD" id="1500"/>
    <dbReference type="GeneTree" id="ENSGT00940000156045"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000007">
        <property type="entry name" value="ARVCF, delta catenin family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028435">
        <property type="entry name" value="Plakophilin/d_Catenin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372:SF6">
        <property type="entry name" value="CATENIN DELTA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10372">
        <property type="entry name" value="PLAKOPHILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00514">
        <property type="entry name" value="Arm"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50176">
        <property type="entry name" value="ARM_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="8" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ARM" evidence="10">
        <location>
            <begin position="408"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="10">
        <location>
            <begin position="451"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="repeat" description="ARM" evidence="10">
        <location>
            <begin position="710"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="855"/>
            <end position="938"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="875"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="889"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="909"/>
            <end position="922"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005002527.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="938" mass="104982" checksum="0779C76CDB2BC3A1" modified="2023-09-13" version="2">MDDSEVESTASILASVKEQEAQFEKLTRALEEERRHVSAQLERVRVSPQDANPLMANGTLTRRHQNGRFVGDADLERQKFSDLKLNGPQDHSHLLYSTIPRMQEPGQIVETYTEEDPEGAMSVVSVETSDDGTTRRTETTVKKVVKTVTTRTVQPVPMGPDGLPVDASSVSNNYIQTLGRDFRKNGNGGPGPYVGQAGTATLPRNFHYPPDGYSRHYEDGYPSSSDNYGSLSRVTRIEERYRPSMEGYRAPSRQDVYGPQPQVRVGGSSVDLHRFHPEPYGLEDDQRSVAYDDLDYGMMSDYGTARRTGTPSDPRRRLRSYEDMIGEEVPSDQYYWAPLAQHERGSLASLDSLRKGGPPPPNWRQPELPEVIAMLGFRLDAVKSNAAAYLQHLCYRNDKVKTDVRKLKGIPVLVGLLDHPKKEVHLGACGALKNISFGRDQDNKIAIKNCDGVPALVRLLRKARDMDLTEVITGTLWNLSSHDSIKMEIVDHALHALTDEVIIPHSGWEREPNEDCKPRHIEWESVLTNTAGCLRNVSSERSEARRKLRECDGLVDALIFIVQAEIGQKDSDSKLVENCVCLLRNLSYQVHREIPQAERYQEAPPNVANNAGPHAASCFGAKKGKDEWFSRGKKPAEDPANDTVDFPKRTSPARGYELLFQPEVVRIYISLLKESKTPAILEASAGAIQNLCAGRWTYGRYIRSALRQEKALSAIADLLTNEHERVVKAASGALRNLAVDARNKELIGKHAIPNLVKNLPERQQSSSQNFSEDTVVSILNTINEVIADNLEAAKKLRETQGIEKLVLINKSGNRSEKEVRAAALVLQTIWGYKELRKPLEKEGWKKSDFQVNLHNASRSQSSHSYDDSTLPLIDRNQKSDKKPDREEIQMSNMGSNTKSLDNNYSTLNERGDHSRTLDRSGDLGEMEPLKGTPLMQKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L5Y0</accession>
    <name>A0A8C4L5Y0_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">ALX homeobox 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ALX3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the paired homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014690674.1">
        <property type="nucleotide sequence ID" value="XM_014835188.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L5Y0"/>
    <dbReference type="Ensembl" id="ENSEAST00005007975.2">
        <property type="protein sequence ID" value="ENSEASP00005007309.2"/>
        <property type="gene ID" value="ENSEASG00005005343.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106827383"/>
    <dbReference type="KEGG" id="eai:106827383"/>
    <dbReference type="CTD" id="257"/>
    <dbReference type="GeneTree" id="ENSGT00940000160669"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035115">
        <property type="term" value="P:embryonic forelimb morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035116">
        <property type="term" value="P:embryonic hindlimb morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007389">
        <property type="term" value="P:pattern specification process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042981">
        <property type="term" value="P:regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000093">
        <property type="entry name" value="ALX homeobox protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050649">
        <property type="entry name" value="Paired_Homeobox_TFs"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24329:SF578">
        <property type="entry name" value="HOMEOBOX PROTEIN ARISTALESS-LIKE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="151"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="153"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007309.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="343" mass="36990" checksum="C0A1BA1AFA5CCFDD" modified="2023-09-13" version="2">MDPERCAPFGVGPASGRYEAAGDEPPGSQGTPATAPHLHPAPPRGPRLIRFPACGPLEPYLPEPAKPPAKYLQDLGPGPALNGGHFYEGPAEAEEKASKAASFPQLPLDCRGGPRDGPSNLQGSPGPCLASLRVPLSPGLPDSMELAKNKSKKRRNRTTFSTFQLEELEKVFQKTHYPDVYAREQLALRTDLTEARVQVWFQNRRAKWRKRERYGKIQEGRNPFTSAYDISMLPRTDSHPQLQNCLWPSPGSGSPGGPCLASPEGIPSPCMSPYSHSHGNVAGFMGVPASPGAHPGIYSIHGFSPPLGGHSFEPASDGDYKSPSLVSLRVKPKEPPSLLNWTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L7I7</accession>
    <name>A0A8C4L7I7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein amnionless</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">AMN</name>
    </gene>
    <organism evidence="12 13">
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        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L7I7"/>
    <dbReference type="Ensembl" id="ENSEAST00005005900.2">
        <property type="protein sequence ID" value="ENSEASP00005005395.2"/>
        <property type="gene ID" value="ENSEASG00005004014.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007463"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031528">
        <property type="term" value="C:microvillus membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038024">
        <property type="term" value="F:cargo receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009235">
        <property type="term" value="P:cobalamin metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015889">
        <property type="term" value="P:cobalamin transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006898">
        <property type="term" value="P:receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026112">
        <property type="entry name" value="AMN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14995">
        <property type="entry name" value="AMNIONLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14995:SF2">
        <property type="entry name" value="PROTEIN AMNIONLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14828">
        <property type="entry name" value="Amnionless"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="458"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005395.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="544" mass="56545" checksum="5B9085B5954A6664" modified="2023-09-13" version="2">ACGEAGATPRPPTLSVTPGTPPQNRSFLSSLTRFRAALRLPSGFGSWNPRAPGLYSFSGVTGEGNGPGKCYQAPNSTLPGLRLGPARWPAALLPLAYLTLSQGEAPPGPDLPCPLCGHDLGSPPQGRGAGVGAEREPLAPWTSAWRRLSLRFLQLLPLDGEFVLASGARFSAPDASSDSDCSAGAPALFLDPDRFSWHDPSVWRSGHAARGLFSVDSERVPCRHDDVVFPSDASFRVGLGLGARTVRVRSVWALGQTFTRDEDLAAFLASRAGRLRFHGPGALSVGSEACADPSGCVCGNAEVQPWICEALLQPLGGHCPPAACHDALRPEGQCCDLCGAIVLLTHGPAFDLDRYRARLLDSFLALPQYQGLQMAVSKVPRTPQSREATGAEADTEIQVVLAETGTETGGAGRLARALLADVAEHGEALGVLSATARESGAPVGDGSAAAQGGPAPRAALAGGVVAALLLLLALLAGALLLRRAGRLRWRRRDEAAPAPAGAPLGFDNPVFDVAGSAEQPPAPQADARSTSRSYFVNPLFEAEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L7M1</accession>
    <name>A0A8C4L7M1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Transferrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">TF</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the transferrin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L7M1"/>
    <dbReference type="Ensembl" id="ENSEAST00005005938.2">
        <property type="protein sequence ID" value="ENSEASP00005005433.2"/>
        <property type="gene ID" value="ENSEASG00005003981.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154388"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009925">
        <property type="term" value="C:basal plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030139">
        <property type="term" value="C:endocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990712">
        <property type="term" value="C:HFE-transferrin receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019899">
        <property type="term" value="F:enzyme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034986">
        <property type="term" value="F:iron chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990459">
        <property type="term" value="F:transferrin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019731">
        <property type="term" value="P:antibacterial humoral response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071281">
        <property type="term" value="P:cellular response to iron ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060586">
        <property type="term" value="P:multicellular organismal-level iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051241">
        <property type="term" value="P:negative regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030316">
        <property type="term" value="P:osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048260">
        <property type="term" value="P:positive regulation of receptor-mediated endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd13618">
        <property type="entry name" value="PBP2_transferrin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000095">
        <property type="entry name" value="Lactotransferrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000105">
        <property type="entry name" value="Serotransferrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016357">
        <property type="entry name" value="Transferrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001156">
        <property type="entry name" value="Transferrin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018195">
        <property type="entry name" value="Transferrin_Fe_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11485:SF31">
        <property type="entry name" value="SEROTRANSFERRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11485">
        <property type="entry name" value="TRANSFERRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00405">
        <property type="entry name" value="Transferrin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002549">
        <property type="entry name" value="Transferrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00422">
        <property type="entry name" value="TRANSFERRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00094">
        <property type="entry name" value="TR_FER"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00205">
        <property type="entry name" value="TRANSFERRIN_LIKE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00206">
        <property type="entry name" value="TRANSFERRIN_LIKE_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00207">
        <property type="entry name" value="TRANSFERRIN_LIKE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51408">
        <property type="entry name" value="TRANSFERRIN_LIKE_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 14" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0410">Iron transport</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 16" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="16">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040226389" evidence="16">
        <location>
            <begin position="20"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin-like" evidence="17">
        <location>
            <begin position="23"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Transferrin-like" evidence="17">
        <location>
            <begin position="363"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>hydrogencarbonate</name>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <label>1</label>
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        <location>
            <position position="270"/>
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        <ligand>
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        <location>
            <position position="476"/>
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        <ligand>
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    </feature>
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        <location>
            <position position="480"/>
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        <ligand>
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        </ligand>
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        <ligand>
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        <location>
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        <ligand>
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            <label>1</label>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L816</accession>
    <name>A0A8C4L816_EQUAS</name>
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        <submittedName>
            <fullName evidence="10">LETM1 domain containing 1</fullName>
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        <name evidence="10" type="primary">LETMD1</name>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
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                <person name="Yue"/>
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                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
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                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="LETM1-like_RBD"/>
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        <property type="entry name" value="LETM1/MDM38-like"/>
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        <property type="entry name" value="LETM1 DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14009">
        <property type="entry name" value="LEUCINE ZIPPER-EF-HAND CONTAINING TRANSMEMBRANE PROTEIN"/>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="Letm1 RBD" evidence="9">
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            <begin position="199"/>
            <end position="373"/>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51758"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008254.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="373" mass="43182" checksum="590DA77D4EB9DAD0" modified="2023-09-13" version="2">MALSRVCWARAALWGSAVTPGLYVTRKLQLVRSGLAWGAPGSSKLHLSPKTDVKNLISYVVTKTKVINGKYHRFLGRHFPRFYVLYTIFMKGLQMLWADAKKARRIKTNMWKHNIKFHQLSYREMEHLRQVWAGGRYPEVHGEFRRDVTKCLFLGIISIPPFANYLVFLLMYLFPRQLLIRHFWTPKQQIDFLDIYHALRKQSHPEILCCLERVIPLISDAGLRWHMAELCTKIQHGTHPAVHDILALRNCFSNYPLGMNQLHALQMKALSRAMLLTPYLPPLLLRHRLKTHTTVIHHLDKALAKLGIGQLTAQEVKSACYLRGLNSTHVAEDRCRTWLGEWLQISCSLKEAELSLLLHNVVLLSTNYIGTRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L8D6</accession>
    <name>A0A8C4L8D6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="29">N-fatty-acyl-amino acid synthase/hydrolase PM20D1</fullName>
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            <ecNumber evidence="3">3.5.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="30">Peptidase M20 domain-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="33" type="primary">PM20D1</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Secreted enzyme that regulates the endogenous N-fatty acyl amino acid (NAAs) tissue and circulating levels by functioning as a bidirectional NAA synthase/hydrolase. It condenses free fatty acids and free amino acids to generate NAAs and bidirectionally catalyzes the reverse hydrolysis reaction. Some of these NAAs stimulate oxidative metabolism via mitochondrial uncoupling, increasing energy expenditure in a UPC1-independent manner. Thereby, this secreted protein may indirectly regulate whole body energy expenditure. PM20D1 circulates in tight association with both low- and high-density (LDL and HDL,respectively) lipoprotein particles.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoate + L-phenylalanine = N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-L-phenylalanine + H2O</text>
            <dbReference type="Rhea" id="RHEA:51312"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:134022"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:51313"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="15">
            <dbReference type="Rhea" id="RHEA:51314"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(9Z)-octadecenoate + glycine = N-(9Z-octadecenoyl)glycine + H2O</text>
            <dbReference type="Rhea" id="RHEA:51316"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:133992"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="11">
            <dbReference type="Rhea" id="RHEA:51318"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>L-phenylalanine + (9Z)-octadecenoate = N-(9Z-octadecenoyl)-L-phenylalanine + H2O</text>
            <dbReference type="Rhea" id="RHEA:51300"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:134020"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:51301"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="26">
            <dbReference type="Rhea" id="RHEA:51302"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-L-phenylalanine + H2O = (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + L-phenylalanine</text>
            <dbReference type="Rhea" id="RHEA:64132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:77016"/>
            <dbReference type="ChEBI" id="CHEBI:149701"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:64133"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycine</text>
            <dbReference type="Rhea" id="RHEA:64108"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:59002"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:64109"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="19">
            <dbReference type="Rhea" id="RHEA:64110"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-L-serine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + L-serine</text>
            <dbReference type="Rhea" id="RHEA:64116"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:149697"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:64117"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="27">
            <dbReference type="Rhea" id="RHEA:64118"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>N-(9Z-octadecenoyl)-L-asparagine + H2O = L-asparagine + (9Z)-octadecenoate</text>
            <dbReference type="Rhea" id="RHEA:64136"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:149730"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:64137"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>N-(9Z-octadecenoyl)-L-glutamine + H2O = L-glutamine + (9Z)-octadecenoate</text>
            <dbReference type="Rhea" id="RHEA:51356"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:134033"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:51357"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>N-(9Z-octadecenoyl)-L-leucine + H2O = L-leucine + (9Z)-octadecenoate</text>
            <dbReference type="Rhea" id="RHEA:51360"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="ChEBI" id="CHEBI:134035"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:51361"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="24">
            <dbReference type="Rhea" id="RHEA:51362"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>N-(9Z-octadecenoyl)-L-lysine + H2O = L-lysine + (9Z)-octadecenoate</text>
            <dbReference type="Rhea" id="RHEA:64192"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:149731"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:64193"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>N-(9Z-octadecenoyl)-L-methionine + H2O = (9Z)-octadecenoate + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:64144"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:149732"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:64145"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>N-(9Z-octadecenoyl)-L-serine + H2O = L-serine + (9Z)-octadecenoate</text>
            <dbReference type="Rhea" id="RHEA:51352"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:134031"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:51353"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>N-(9Z-octadecenoyl)-L-tryptophan + H2O = L-tryptophan + (9Z)-octadecenoate</text>
            <dbReference type="Rhea" id="RHEA:64176"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:149733"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:64177"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N-(9Z-octadecenoyl)-L-tyrosine + H2O = L-tyrosine + (9Z)-octadecenoate</text>
            <dbReference type="Rhea" id="RHEA:64184"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:149734"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:64185"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N-hexadecanoyl-L-phenylalanine + H2O = hexadecanoate + L-phenylalanine</text>
            <dbReference type="Rhea" id="RHEA:64124"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:149699"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:64125"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N-octadecanoyl-L-phenylalanine + H2O = octadecanoate + L-phenylalanine</text>
            <dbReference type="Rhea" id="RHEA:64128"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:149700"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:64129"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>an N-acyl-L-amino acid + H2O = an L-alpha-amino acid + a carboxylate</text>
            <dbReference type="Rhea" id="RHEA:15565"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="EC" id="3.5.1.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:15566"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="20">
            <dbReference type="Rhea" id="RHEA:15567"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>an N-acyl-aromatic L-alpha-amino acid + H2O = an aromatic L-alpha-amino acid + a carboxylate</text>
            <dbReference type="Rhea" id="RHEA:54184"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:84824"/>
            <dbReference type="ChEBI" id="CHEBI:138093"/>
            <dbReference type="EC" id="3.5.1.114"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:54185"/>
        </physiologicalReaction>
        <physiologicalReaction direction="right-to-left" evidence="25">
            <dbReference type="Rhea" id="RHEA:54186"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M20A family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.114" evidence="9"/>
    <dbReference type="EC" id="3.5.1.14" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4L8D6"/>
    <dbReference type="Ensembl" id="ENSEAST00005008618.2">
        <property type="protein sequence ID" value="ENSEASP00005007899.2"/>
        <property type="gene ID" value="ENSEASG00005005744.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156659"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004046">
        <property type="term" value="F:aminoacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990845">
        <property type="term" value="P:adaptive thermogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043604">
        <property type="term" value="P:amide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043605">
        <property type="term" value="P:amide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097009">
        <property type="term" value="P:energy homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05674">
        <property type="entry name" value="M20_yscS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.900:FF:000003">
        <property type="entry name" value="N-fatty-acyl-amino acid synthase/hydrolase PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.10:FF:000027">
        <property type="entry name" value="N-fatty-acyl-amino acid synthase/hydrolase PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047177">
        <property type="entry name" value="Pept_M20A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE/HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45962:SF1">
        <property type="entry name" value="N-FATTY-ACYL-AMINO ACID SYNTHASE_HYDROLASE PM20D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="31" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="31">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040434395" description="N-fatty-acyl-amino acid synthase/hydrolase PM20D1" evidence="31">
        <location>
            <begin position="26"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="32">
        <location>
            <begin position="241"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="ARBA" id="ARBA00034807"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="ARBA" id="ARBA00046147"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047450"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047567"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047866"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047874"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047879"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048145"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
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            <dbReference type="ARBA" id="ARBA00048380"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
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            <dbReference type="ARBA" id="ARBA00048402"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048579"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00048597"/>
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    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00048729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
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            <dbReference type="ARBA" id="ARBA00048822"/>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
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            <dbReference type="ARBA" id="ARBA00048827"/>
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    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048840"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00048879"/>
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    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00049100"/>
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    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00049457"/>
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    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00069960"/>
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    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00079007"/>
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    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
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    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007899.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="503" mass="55449" checksum="3268C80EF930337F" modified="2023-09-13" version="2">MARGYVCVLALAAVLLLGIAAVSRSKGLRGKEPQRASRVPSEFSQGERVAMKEGLKGAIQIPTVSFSPNEFNTTALAEFGEYIHEVFPTVFKTSFIQHEVVGEYSHLFTVQGSDPNLQPYMLLAHIDVVPAPDEGWEVPPFSGLERDGFIYGRGTLDNKNSVMAILQALELLLIRSYVPQRSFFIALGHDEEVSGANGAQKISALLQARGIQLAFIVDEGSFILDGLVPNLKKPFAMVAVSEKGMLNLELQVNMTPGHSSAPPEETSIGILAAAVSRLEQTPMPNMFGGGPLKMALQQLANEFPFPINIVLSNLWLFRPLVSRLMERNKITSALVRTTTAVTMFKAGIKVNVIPSVAQATVNFRIHPAQTVQQVLELVKNIVADDRVQFHVLNAIDPLPNSPSDSQAFGYQLLHQTIQSVFPEVHIVVPGICIGNTDSRHYTNLTTGIYRFNPVYLQPQGSYGIHGRNEKISVQAYETQVKFIFEFIQNADRDWVLVPHQHEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L9P0</accession>
    <name>A0A8C4L9P0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">ADAM metallopeptidase domain 20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106824088</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L9P0"/>
    <dbReference type="Ensembl" id="ENSEAST00005006477.2">
        <property type="protein sequence ID" value="ENSEASP00005005923.2"/>
        <property type="gene ID" value="ENSEASG00005028455.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005068233.1">
        <property type="protein sequence ID" value="ENSEASP00005059596.1"/>
        <property type="gene ID" value="ENSEASG00005028455.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005080816.1">
        <property type="protein sequence ID" value="ENSEASP00005050122.1"/>
        <property type="gene ID" value="ENSEASG00005028455.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161067"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990913">
        <property type="term" value="C:sperm head plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008584">
        <property type="term" value="P:male gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04269">
        <property type="entry name" value="ZnMc_adamalysin_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.390.10:FF:000002">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.70.10:FF:000001">
        <property type="entry name" value="Disintegrin and metalloproteinase domain-containing protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006586">
        <property type="entry name" value="ADAM_Cys-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018358">
        <property type="entry name" value="Disintegrin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002870">
        <property type="entry name" value="Peptidase_M12B_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034027">
        <property type="entry name" value="Reprolysin_adamalysin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF232">
        <property type="entry name" value="DISINTEGRIN AND METALLOPROTEINASE DOMAIN-CONTAINING PROTEIN 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08516">
        <property type="entry name" value="ADAM_CR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01562">
        <property type="entry name" value="Pep_M12B_propep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01421">
        <property type="entry name" value="Reprolysin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00289">
        <property type="entry name" value="DISINTEGRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00608">
        <property type="entry name" value="ACR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00427">
        <property type="entry name" value="DISINTEGRIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044680394" description="ADAM metallopeptidase domain 20" evidence="9">
        <location>
            <begin position="26"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="12">
        <location>
            <begin position="208"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="11">
        <location>
            <begin position="405"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="10">
        <location>
            <begin position="629"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="342"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="346"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="352"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="8">
        <location>
            <begin position="359"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="463"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="652"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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    </evidence>
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            <dbReference type="Google" id="ProtNLM"/>
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</entry>
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            <fullName evidence="9">Tether containing UBX domain for GLUT4</fullName>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Eutheria</taxon>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="12" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
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            <topology evidence="8">Peripheral membrane protein</topology>
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        <subcellularLocation>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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    </dbReference>
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        <property type="entry name" value="UBX1_UBXN9"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021569">
        <property type="entry name" value="TUG-UBL1"/>
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    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
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    <dbReference type="InterPro" id="IPR059238">
        <property type="entry name" value="UBX1_UBXN9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001012">
        <property type="entry name" value="UBX_dom"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="Pfam" id="PF11470">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="390"/>
            <end position="466"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="176"/>
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            <begin position="503"/>
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            <begin position="203"/>
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                <person name="Yang"/>
                <person name="C."/>
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                <person name="Y."/>
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                <person name="Gao"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
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                <person name="Li"/>
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    <reference evidence="4" key="2">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
            </authorList>
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        <property type="entry name" value="Immunoglobulin heavy constant gamma 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulin heavy constant gamma 1"/>
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        <property type="entry name" value="Immunoglobulin heavy constant gamma 1"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
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    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR003006">
        <property type="entry name" value="Ig/MHC_CS"/>
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    <dbReference type="InterPro" id="IPR003597">
        <property type="entry name" value="Ig_C1-set"/>
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    <dbReference type="InterPro" id="IPR050380">
        <property type="entry name" value="Immune_Resp_Modulators"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23411">
        <property type="entry name" value="TAPASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07654">
        <property type="entry name" value="C1-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00407">
        <property type="entry name" value="IGc1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00290">
        <property type="entry name" value="IG_MHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="161"/>
            <end position="264"/>
        </location>
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    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="280"/>
            <end position="379"/>
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    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="388"/>
            <end position="494"/>
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        <location>
            <begin position="1"/>
            <end position="33"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="50"/>
            <end position="75"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="135"/>
            <end position="165"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="146"/>
            <end position="165"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006421.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="499" mass="54512" checksum="A0F31F5B8F4E4817" modified="2023-09-13" version="2">MEGGPWDGRSLGVKGPGASLRGPDRIQFSGPGTPVLELVGTLDRRGRRAEAVWGDTRQGSRQKEERGPHWGLSWGCQHGERPGRALGLSRQASVRGDRTGLGVRGRGSTLAHTGFWGGWHLEERNARGQRMASRLHRGASCAGEGPRLEEGSAVSKELDRPKSREGGGQAALVFQLTPSCGITSDATVALGCLVSDYYPEPVTVSWNSGALTSGVHTFPSVLQSSGLYALSSMVTVPASTWTSETYICNVAHPASSTKVDKRISEWRGRREGIPCETGGPSVFIFPPNPKDALMISRTPVVTCVVVNLSDQYPDVQFSWYVDNTEVHSAITKQRELQSNSTYRVVSVLPIQHQDWLSGKEFKCSVTNIGVPQPISRAISRGKGEPRVPQVYVLPPHPDELAKSKVSVTCLVKDFYPPDISVEWQSNRWPELEGKYSTTPAQLDSDGSYFLYSKLSLETSRWQQEVIQQVAAGESFTCAVMHEALHNHFTKTDISESLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LF24</accession>
    <name>A0A8C4LF24_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phosphodiesterase</fullName>
            <ecNumber evidence="16">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">PDE3B</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:25278"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>3',5'-cyclic GMP + H2O = GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16957"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57746"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:16958"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a nucleoside 3',5'-cyclic phosphate + H2O = a nucleoside 5'-phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14653"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57867"/>
            <dbReference type="ChEBI" id="CHEBI:58464"/>
            <dbReference type="EC" id="3.1.4.17"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:14654"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="16">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="16"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4LF24"/>
    <dbReference type="Ensembl" id="ENSEAST00005009053.2">
        <property type="protein sequence ID" value="ENSEASP00005008306.1"/>
        <property type="gene ID" value="ENSEASG00005005968.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159336"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032045">
        <property type="term" value="C:guanyl-nucleotide exchange factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016525">
        <property type="term" value="P:negative regulation of angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007162">
        <property type="term" value="P:negative regulation of cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050995">
        <property type="term" value="P:negative regulation of lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000008">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0114">cAMP</keyword>
    <keyword evidence="4" id="KW-0140">cGMP</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="111"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="195"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="domain" description="PDEase" evidence="19">
        <location>
            <begin position="656"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="420"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1023"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1089"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="430"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="458"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1023"/>
            <end position="1048"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1109"/>
            <end position="1123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1123" mass="125133" checksum="6613F650DE6A62F1" modified="2022-01-19" version="1">MRRDERDAKAMRCPPPPDRAGSPPESLRNGYVKSCVSPLRQDPPRGFFFHLCRLCNVELLLPPPASPLQPRRRSPFSRARLSLGALAAFVLALLLGSGLEGWAAGAARLRTLLSVCSLSLSPLFSIACAFFFLTCFLTRTKHGPGPGPGPGGGAWWLLALPACCYLGDFLVWQCWSWSWGPGEAGAPAPHTPPAVAGRLLWVLGCVGLLTLARPLRLRHSILVLLFSSCVWWVSFTSLGALPSALRPLLSCLAGGTGCLLALGLDHFFQIREAPHHPRLSSTAEEKVPVIRPRRRSSCVSFGETSASYYGSSKMFRRPSLPCISREQMILWDWDLKQWYKPHYQNSGGGSGVDLSVLNEARKMVSDLLVDPTLPPQVISSLRSISSLMGAFSGSCRPKINPLTPFPGFYPCSEIEDPAEKGDRKLHKGLNSRNSLPTPQLRRSSGTSGLLPVEQSSSRWERNNGKRPHQEFGISSQGCCLNGPFSSNLLTIPKQRSSSVSLTHHIGLRRAGALPSLSPVNSLSHGSVSAGSLTSRSPIEFPDTADFLTKPSVVLHRSLGNAPNSPDFYQQLRNSDSNLCNSCGHQILNYVSTSESESGTDCHSGKSGDEDNTIFSKESFSITEAQHEVETENRDSRKLFLEGDNHLTEEAQNEQQPNIEQEASLDLILMEDYDSLIEKMSNWNFQIFELVEKMGEKSGRILSQVMYALFQDTGLLEIFKIPTLQFMNYFRALENGYRDIPYHNRIHATDVLHAVWYLTTRPIPGLQQTHNDPATGNETDSDGRISPEQTAYISSRSCSIPDESYGCLSANIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASAWNLYLSRPEYNFLLNLDHVEFKRFRFLVIEAILATDLKKHFDFLAEFNAKANDVNSNGIEWSNENDRLLVCQVCIKLADINGPAKVRDLHLKWTEGIVNEFYEQGDEEANLGLPISPFMDRSSPQLAKLQESFITHIVGPLCNSYDAAGLLPGQWIEAEEDDDAESCYEEEDGEELDTDEEEIEDNLNPKPQRRKGRRRIFCQLMHHLAENHKIWKEIIEEEEKCKSDGNKLQVENSSLPQADEIQVIEEADEEEERQFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LHF9</accession>
    <name>A0A8C4LHF9_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">AT-hook containing transcription factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">AHCTF1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005008809.2">
        <property type="protein sequence ID" value="ENSEASP00005008075.1"/>
        <property type="gene ID" value="ENSEASG00005005859.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018900"/>
    <dbReference type="OMA" id="KWNHDCL"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032040">
        <property type="entry name" value="ELYS-bb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052620">
        <property type="entry name" value="ELYS/MEL-28_NucAsmblyFactor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025151">
        <property type="entry name" value="ELYS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21583">
        <property type="entry name" value="ELYS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21583:SF8">
        <property type="entry name" value="PROTEIN ELYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13934">
        <property type="entry name" value="ELYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16687">
        <property type="entry name" value="ELYS-bb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ELYS beta-propeller" evidence="5">
        <location>
            <begin position="1"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="ELYS-like" evidence="4">
        <location>
            <begin position="722"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1288"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1793"/>
            <end position="1821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1873"/>
            <end position="2083"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2185"/>
            <end position="2256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1289"/>
            <end position="1300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1316"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1802"/>
            <end position="1815"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1948"/>
            <end position="1957"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1998"/>
            <end position="2009"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="2071"/>
            <end position="2083"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="2198"/>
            <end position="2207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF16687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="2275" mass="252325" checksum="4772C1511542414C" modified="2022-01-19" version="1">MRDLTAQVTSALLQFPEVTVQALGEEEIRLEAVLRGKFAAGKNGLACLACGPQLEVVNSLTGERLSAYRFSGADGQSPIVLAVKEFSWQKRTGLLIGLEDAEGSVLCLYDLGTSRVVKAVVLPGRVTAIEPIINHGGASASTQHLHPSLRWLFGVAAVVTDVGQILLIDLCLDDLSCSQNEVEASDLEVVTGIPAEVPHFRESVMREGRHLCFQLLNPSGTAVSTLSYINRTNQLAIGFSDGYLALWNMKSMKREYYTQLEGGRVPVYAVTFQEPENDPCNCCYLWAVQSTQDSEGDVLSLHLLQLAFGDRKCLASGQILYEGLEYCEERYTLDLTGGVFPLRGQTSNTKLLGCQSIEKFRSHGDREDSVNEALSPDTSVSVFTWQVNIYGQGKPSMYLGLFDINRWYHAQMPDSLRSGEYLHNCSYFALWSLDAVVSRTSPHYVLDILVHERSLSRGLPPSYPPPEQFFNPSAYNFDATCLLNSGVVHITCTGFQKETLTFLKKSGSSLNELIPDSYNRCLVAGLLSPRLIDIQPSSLSQEEQLEAILSAAIHTSSLGLLTGCITRWIAEEQPNSAANLHFVLEWTWNKVVLTKEEFDRLCTPLFDGSCRFIDSQTIQSLQQCHLLLSNLSTVLSCFATEAQEITERGLMDLSNKCMVTQLICQYAQVVLWFSHSGLLPEGLDDAVQLSRLCYNYPVIQNYYTSRRQKCERLSRGKWSPDCLMIDEMVSQLGVGVEKLWKRDEGGTGRYPPTSLHALLDIYLLDNVTEASKHSITIYLLLDIMYSFPNKTDTSIESFPTAFAISWGQVKLIQGFWLIDHNDYESGLDLLFHPATAKPLSWQHSKIIQAFMSQGEHRQALRYIQTMKPTVSSGSDVILHLTVLLFNRCMVEAWSLLRQHSTSLNIEELLKHTYEVCQEMGLMEDLLKLPFTHTEQECLVRFLQSSTSVQNHEFLLVHHLQRANYIPALKLNQTLKINLMNDRDPRLRERSLARNSILDQYGKILPRVQRKLAIERAKPYHLSTSSVLREVSRPKPLSAVPKQAVTGTVLTRSTFINNVLSKIGEVWASNEPKNSISAYSSSKIEDPCPIVYSLPEPEPPEAFVGTPISKASEKISRLLDLVVHPVAQPSQCLGFIQQSPTRSPLYLASSSLPVNSQSKVSQQNTSRASELQLLETPLVVKKAKTLAMSVTSSGLSDFTPQSILRSGLRATPLASPSLSPGRSLTPPLRLKETRISFMEEGMTVKWTSGAADDSKTKIFVTTPLHECGVPAETEWLKNKDKAISFSLNSPEKDHQEMDVRSQDTPSQSLEKLDMSKENSSISTRSDQTTLEYQDAPSPEDFEDIFMATKPASSSTELIPNLTEQTEKDSDNDVFESEVTPPDLEKQMGTLEDSETKDLLVAEEALAELNHLSHVKGVEASLQSFHEGKSLTLKPKVPVLDEGLVSVETCASAVRAGDDKSVPGILGDSSSSGLVISEGPVVSERRLDQEVALGLEEDRAVEVDALKASVDLPEEKPPISDDPPNTQEVHVIEREELEVQDSGQEARSLSFNELYPSGTLKLQYNFDTIEQQFCDLPDNKDSAECDIAEVDGELFVAQSNFTLILEGEEGEVETGDSAASNVLAKAANTATEEKHVYSGENDNHGHAASLPSVITSDHESQKVETLPYVPEPIKVAIAENLLDVIKDTRSKEITSDTMEQSICENRPLISQKVMCSTKLVKSTCKTVQEASTVTINVSPVNDMISSRTRTRGQRVRNLNVRSAQEEESADVAAPELLGLSVTKKSRKTKEISEASESACADVKGTSQNQQIPQNSVTPRRGRRKREINQDTLESINSMEQGLQVTPGRELRRLKSSQLVEPATEETSFRKEVKLSSVTKRTPKRAKKSVENQESVEIVSDLKVSKVASPGRSTRKLRSANLEASEKAGYEQAGKSIEQQLPTQHPRRVQKREVGASHVEDDSELESSQLTLRAEVDTPATPRKRGRPRKVNPSQDVGSEAVKEVSPKKRETPSIPRRSTRNTPAKRERADVGKPASEKSILVPNEELAPVMSSKKKLTKRTENQSQKHPLHSISEKCVDEEITRKEPNDQEEKLLVTAALTKSSRSTRTRSSKAILLPDLSEPKNESFLFSPPLSKVPRKEKAKKIEAPEQLKELVSDLSSQFVFSPPTLRTRRKNASNTSKLVDELEDDAQSVEAVGKQKVKRARTAKTKQASKNTEKESWSPPPVEIKLISPLASPIDGIKSKPRKTAEVTGKALGRSRKKLSAFPKQVLRRKML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LIA6</accession>
    <name>A0A8C4LIA6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106843510</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LIA6"/>
    <dbReference type="Ensembl" id="ENSEAST00005013405.2">
        <property type="protein sequence ID" value="ENSEASP00005012343.2"/>
        <property type="gene ID" value="ENSEASG00005008612.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106843510"/>
    <dbReference type="KEGG" id="eai:106843510"/>
    <dbReference type="GeneTree" id="ENSGT01140000282520"/>
    <dbReference type="OrthoDB" id="9975554at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
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    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="25"/>
            <end position="48"/>
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            <begin position="60"/>
            <end position="80"/>
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            <begin position="41"/>
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            <dbReference type="RuleBase" id="RU363047"/>
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            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="317" mass="35875" checksum="D8700B6ADA6A4A68" modified="2023-09-13" version="2">MEGKNQTVLSEFIILGFSNLSELQFLLFTIFFLTYLCTLGGNIFIILVTMADPHLHTPMYHFLGNLAFLDICYTTTNVPQMMVHLVSKKKSISFWGCVAQLFAFIFFVGSECLLLAAMAYDRYIAIRKPLRYSVIMNKVLYSQLAASCWTGGFLNSVVHTVLTFRLPFCGNNQINYFFCDIPPLLILSCGDTSVNELALLSIGVFIGWIPFLCIVLSYLYITSAILRIRSSEGRRKAFSTCASHLIIVLLYYGSAIFTYVRPISAYSLEKDRLISVLYSVVTPTLNPIIYTLRNKDIREAVKAVGRKWQPPIPSFNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LKX4</accession>
    <name>A0A8C4LKX4_EQUAS</name>
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        <recommendedName>
            <fullName evidence="9">Peptidase A1 domain-containing protein</fullName>
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        <name evidence="10" type="primary">LOC106844376</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
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                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="G."/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peptidase A1 family.</text>
    </comment>
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        <property type="gene ID" value="ENSEASG00005008103.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 17"/>
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        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:aspartic-type endopeptidase activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Cathepsin E"/>
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        <property type="entry name" value="Pepsin A"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
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        <property type="entry name" value="Aspartic_peptidase_AS"/>
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    <dbReference type="InterPro" id="IPR012848">
        <property type="entry name" value="Aspartic_peptidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
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    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PEPSIN A-5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="A1_Propeptide"/>
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    </dbReference>
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        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase A1" evidence="9">
        <location>
            <begin position="75"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="93"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="276"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="106"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="267"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="310"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023157"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="389" mass="42859" checksum="850B7B80D4E5E7CC" modified="2023-09-13" version="2">FLLHVLLGVLLALPPCLCVIPLVKIKSLRENLREKDMLKEYLEKYPFRLTHKLLHKHADSGVAFEPMRNYLDIAYMGIISVGTPPQEFQVIFDTGSADLWVPSIYCSSPACSNHNTFNPLRSSTFVASGQPIKLIYGTGKMSGFVGYDTIKISSLVDRNQAFGLSVEEPDKILELATFDGILGLSYPSLSVKGVTPVFDNLWNQGLLSQKLFAFYLSSKGKKGSVVMFGGVDPSYYTGELHWVPVSKPLYWQISMDSISINGKVIACDGGCQAIVDTGTSLLLGPQDAVLNIQEIIQARRSTSGEYFIDCDAVNTLPDILFTIDGIGYPVPANAYIQKDAALGICFSSFEGNEDISNNSEEWILGDVFLRLYFTVFDRENDRIGLATAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LM71</accession>
    <name>A0A8C4LM71_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cleavage and polyadenylation specificity factor subunit 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CPSF6</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. CPSF6 enhances NUDT21/CPSF5 binding to 5'-UGUA-3' elements localized upstream of pA signals and promotes RNA looping, and hence activates directly the mRNA 3'-processing machinery. Plays a role in mRNA export.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RRM CPSF6/7 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044611522.1">
        <property type="nucleotide sequence ID" value="XM_044755587.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070350812.1">
        <property type="nucleotide sequence ID" value="XM_070494711.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LM71"/>
    <dbReference type="SMR" id="A0A8C4LM71"/>
    <dbReference type="Ensembl" id="ENSEAST00005012461.2">
        <property type="protein sequence ID" value="ENSEASP00005011462.1"/>
        <property type="gene ID" value="ENSEASG00005008066.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106843577"/>
    <dbReference type="CTD" id="11052"/>
    <dbReference type="GeneTree" id="ENSGT00730000110905"/>
    <dbReference type="OMA" id="CTRQNLN"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12643">
        <property type="entry name" value="RRM_CFIm68"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000081">
        <property type="entry name" value="Cleavage and polyadenylation specificity factor subunit 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034772">
        <property type="entry name" value="CPSF6/7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057951">
        <property type="entry name" value="CPSF6/7_RSLD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034769">
        <property type="entry name" value="CPSF6_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23204">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFIC FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25524">
        <property type="entry name" value="RSLD_CPSF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="81"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="169"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="477"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="169"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="207"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="220"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="285"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="377"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="389"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="489"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="504"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="515"/>
            <end position="551"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00057527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="551" mass="59210" checksum="721A5DA1B456AA79" modified="2022-01-19" version="1">MADGVDHIDIYADVGEEFNQEAEYGGHDQIDLYDDVISPSANNGDAPEDRDYMDTLPPTVGDDVGKGAAPNVVYTYTGKRIALYIGNLTWWTTDEDLTEAVHSLGVNDILEIKFFENRANGQSKGFALVGVGSEASSKKLMDLLPKRELHGQNPVVTPCNKQFLSQFEMQSRKTTQSGQMSGEGKAGPPGGSSRAAFPQGGRGRGRFPGAVPGGDRFPGPAGPGGPPPPFPAGQTPPRPPLGPPGPPGPPGPPPPGQVLPPPLAGPPNRGDRPPPPVLFPGQPFGQPPLGPLPPGPPPPVPGYGPPPGPPPPQQGPPPPPGPFPPRPPGPLGPPLTLAPPPHLPGPPPGAPPPAPHVNPAFFPPPTNSGMPTSDSRGPPPTDPYGRPPPYDRGDYGPPGREMDTARTPLSEAEFEEIMNRNRAISSSAISRAVSDASAGDYGSAIETLVTAISLIKQSKVSADDRCKVLISSLQDCLHGIESKSYGSGSRRERSRERDHSRSREKSRRHKSRSRDRHDDYYRERSRERERHRDRDRDRDRERDREREYRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LQB9</accession>
    <name>A0A8C4LQB9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="5">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">LATS1</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Microtubule organizing center</location>
            <location evidence="3">Centrosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4LQB9"/>
    <dbReference type="Ensembl" id="ENSEAST00005013881.2">
        <property type="protein sequence ID" value="ENSEASP00005012773.2"/>
        <property type="gene ID" value="ENSEASG00005008927.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837214"/>
    <dbReference type="KEGG" id="eai:106837214"/>
    <dbReference type="CTD" id="9113"/>
    <dbReference type="GeneTree" id="ENSGT00940000157684"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030331">
        <property type="term" value="F:nuclear estrogen receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000082">
        <property type="term" value="P:G1/S transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000086">
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    <keyword evidence="18" id="KW-0131">Cell cycle</keyword>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LQS4</accession>
    <name>A0A8C4LQS4_EQUAS</name>
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        <recommendedName>
            <fullName evidence="8">Zinc transporter ZIP13</fullName>
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        <alternativeName>
            <fullName evidence="9">Solute carrier family 39 member 13</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Zrt- and Irt-like protein 13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">SLC39A13</name>
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    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Functions as a zinc transporter transporting Zn(2+) from the Golgi apparatus to the cytosol and thus influences the zinc level at least in areas of the cytosol. May regulate beige adipocyte differentiation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Zn(2+)(in) = Zn(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29351"/>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LQS4"/>
    <dbReference type="Ensembl" id="ENSEAST00005016851.2">
        <property type="protein sequence ID" value="ENSEASP00005015493.2"/>
        <property type="gene ID" value="ENSEASG00005010790.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157349"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16950">
        <property type="entry name" value="ZINC TRANSPORTER SLC39A7 HISTIDINE-RICH MEMBRANE PROTEIN KE4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16950:SF16">
        <property type="entry name" value="ZINC TRANSPORTER ZIP13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="3 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0864">Zinc transport</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040294326" description="Zinc transporter ZIP13" evidence="13">
        <location>
            <begin position="44"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="120"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="353"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00042542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00042972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005015493.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="375" mass="39663" checksum="A612D4225F342AFF" modified="2023-09-13" version="2">MKGATREAARGMSGCPCPGCGMAGQRLLFLTALALELLGGAGGSQQALRNRGAAAACRLDNKESESWGALLSGERLDTWICSLLGSLMVGLSGVFPLLVIPLEMGTTLRSEAGARRLKQLLSFALGGLLGNVFLHLLPEAWAYTCSASPGGEGQSLQQQQQLGLWVIAGFLTFLVLEKMFLDSKEKEGTSQAPSKDPAAAAALNGGRYLAQPAAEPGLSAMVRSIKVSGYLNLLANTIDNFTHGLAVAASFLVSKKIGLLTTMAILLHEIPHEVGDFAILLRAGFDRWSAAKLQLSTALGGLLGACFAICTQSPKGVEETVAWILPFTSGGFLYIALVNVLPDLLEEDDPWRSLQQVLLLCTGIVVMVLFSIFIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LR38</accession>
    <name>A0A8C4LR38_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LR38"/>
    <dbReference type="Ensembl" id="ENSEAST00005012734.2">
        <property type="protein sequence ID" value="ENSEASP00005011715.2"/>
        <property type="gene ID" value="ENSEASG00005008225.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00520000061410"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011715.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="164" mass="18252" checksum="E325F09FB51A8AD8" modified="2023-09-13" version="2">TARRIFWGQGGLTRGNIYLPLLNFPAAPVFAFFLADPLGSGSLRLAEESEPVTLKKSSRRPWVLPWRLLLSRLTPLRIRSSENCFSPHMNLTSSSSFGSLHFSSTVSGSRTSGSRKSCWASLWSQFSGTGYLLGSSRRTISSMLLCFWMRSTARLGPMPRMLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LRB7</accession>
    <name>A0A8C4LRB7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Palmitoyltransferase</fullName>
            <ecNumber evidence="7">2.3.1.225</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ZDHHC16</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-cysteinyl-[protein] + hexadecanoyl-CoA = S-hexadecanoyl-L-cysteinyl-[protein] + CoA</text>
            <dbReference type="Rhea" id="RHEA:36683"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="2.3.1.225"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The DHHC domain is required for palmitoyltransferase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the DHHC palmitoyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.225" evidence="7"/>
    <dbReference type="RefSeq" id="XP_014718193.1">
        <property type="nucleotide sequence ID" value="XM_014862707.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044608957.1">
        <property type="nucleotide sequence ID" value="XM_044753022.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070342133.1">
        <property type="nucleotide sequence ID" value="XM_070486032.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070342156.1">
        <property type="nucleotide sequence ID" value="XM_070486055.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070342167.1">
        <property type="nucleotide sequence ID" value="XM_070486066.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070342173.1">
        <property type="nucleotide sequence ID" value="XM_070486072.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070342176.1">
        <property type="nucleotide sequence ID" value="XM_070486075.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070342179.1">
        <property type="nucleotide sequence ID" value="XM_070486078.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070342188.1">
        <property type="nucleotide sequence ID" value="XM_070486087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LRB7"/>
    <dbReference type="Ensembl" id="ENSEAST00005013510.2">
        <property type="protein sequence ID" value="ENSEASP00005012445.1"/>
        <property type="gene ID" value="ENSEASG00005008679.2"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:protein-cysteine S-palmitoyltransferase activity"/>
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    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="75"/>
            <end position="103"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="7">
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            <begin position="115"/>
            <end position="134"/>
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            <begin position="199"/>
            <end position="222"/>
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        <location>
            <begin position="267"/>
            <end position="290"/>
        </location>
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    <feature type="domain" description="Palmitoyltransferase DHHC" evidence="8">
        <location>
            <begin position="155"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU079119"/>
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    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01529"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005012445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="377" mass="43627" checksum="EC08AF541CF86FE5" modified="2022-01-19" version="1">MRGQRSLLLGPARLCLRLLLLLGYRRRRPPLLRGLVQRWRYGKVCLRSLLFNSFGSSDAAIDAAFEPIYWLVDNMIRWCGVVFVVLVIVLTSSIVAIAYLCVLPLILRTYSVPRLCWHFFYSHWNLILIVFHYYQAITTPPGYPPQGRTDIATVSICKKCIYPKPARTHHCSICNRCVLKMDHHCPWLNNCVGHYNHRYFFSFCFFMTLGCVYCSYGSWDLFREAYAAIEKMKQLDKNKLQAVTNQTYHQTPPPTFSFRERVTHKSLVYLWFLCSSVALALGALTVWHAVLISRGETSIERLTNRKESCRLRAKGKVFRNHYNYGCLDNWKVFLGVDTGRHWLTRVLLPSSHLPHGNGMSWDPPPWVTAHSTSVMAV</sequence>
</entry>
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    <accession>A0A8C4LRW9</accession>
    <name>A0A8C4LRW9_EQUAS</name>
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        <recommendedName>
            <fullName evidence="9">Fructose-bisphosphate aldolase</fullName>
            <ecNumber evidence="9">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ALDOC</name>
    </gene>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the class I fructose-bisphosphate aldolase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="9"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4LRW9"/>
    <dbReference type="Ensembl" id="ENSEAST00005015493.2">
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        <property type="gene ID" value="ENSEASG00005009946.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182987"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00183"/>
    </dbReference>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00948">
        <property type="entry name" value="FBP_aldolase_I_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000205">
        <property type="entry name" value="Fructose-bisphosphate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000208">
        <property type="entry name" value="Fructose-bisphosphate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029768">
        <property type="entry name" value="Aldolase_I_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000741">
        <property type="entry name" value="FBA_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11627">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00274">
        <property type="entry name" value="Glycolytic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00158">
        <property type="entry name" value="ALDOLASE_CLASS_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 9" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0704">Schiff base</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU004257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014252.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="336" mass="36205" checksum="28BD7664051AFBB4" modified="2023-09-13" version="2">MPHSYPALSAEQKKELSDIALRIVAPGKGILAADESVGSMAKRLSQIGVENTEENRRLYRQVLFSADDRVKKCIGGVIFFHETLYQKDDNGVPFVRTIQDKGIVVGIKVDKGVVPLAGTDGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKISERTPSALAILENANVLARYASICQQVLAAVYKALSDHHVYLEGTLLKPNMVTPGHACPIKYSPEEIAMATVTALRRSVPPAVPGVTFLSGGQSEEEASLNLNAINRCPLPRPWALTFSYGRALQASALNAWRGQQDNAGAATEEFIKRAEVNGLAAQGKYEGSGEDGGAAAQSLYIANHAY</sequence>
</entry>
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    <accession>A0A8C4LT59</accession>
    <name>A0A8C4LT59_EQUAS</name>
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        <recommendedName>
            <fullName evidence="5">Interferon-related developmental regulator 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">IFRD1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Zhao"/>
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                <person name="S."/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Wang"/>
                <person name="X."/>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
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                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="4">Interacts with PSIP1/LEDGF.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the IFRD family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LT59"/>
    <dbReference type="Ensembl" id="ENSEAST00005013671.2">
        <property type="protein sequence ID" value="ENSEASP00005012584.2"/>
        <property type="gene ID" value="ENSEASG00005008784.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013347"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000259">
        <property type="entry name" value="interferon-related developmental regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039777">
        <property type="entry name" value="IFRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006921">
        <property type="entry name" value="Interferon-rel_develop_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007701">
        <property type="entry name" value="Interferon-rel_develop_reg_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12354">
        <property type="entry name" value="INTERFERON-RELATED DEVELOPMENTAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12354:SF6">
        <property type="entry name" value="INTERFERON-RELATED DEVELOPMENTAL REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05004">
        <property type="entry name" value="IFRD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04836">
        <property type="entry name" value="IFRD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0221">Differentiation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Interferon-related developmental regulator N-terminal" evidence="8">
        <location>
            <begin position="49"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Interferon-related developmental regulator C-terminal" evidence="7">
        <location>
            <begin position="391"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00062183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00068647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005012584.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="450" mass="50146" checksum="E298740F5AE20384" modified="2023-09-13" version="2">MPKNKKRNTPHRGGNGGGGSGAAAATAATAGGQHRNVQPFSDEDASIETMSHCSGYSDPSSFAEDGPEVLDEEGTQEDLEYKLKGFIDLTLDKSAKIRQAALEGIKNALASKMLYEFVLERRMTLTDSIERCLKKGKSDEQRAAAALASVLCIQLGPGIESEEVLKTLGPILKKIICDGTASIQARQTCATCCGVCSFIATDDITELYSTLECLENIFTKSYLKEKNTNVVCSTPNTVLHISSLLAWTLLLTICPINEVKKKLEMHFHKLPSLLSSDDVNMRIAAGESLALLFELARGMDSDFFYEDMESLTQILRALATDGNKHRAKVDKRKQRSVFRDILRAVEERDFPTETVKFGPERMYIDCWVKKHTYDTFKEVLGSGMQYHLQSNEFLRNVFELGPPVMLDAAMLKTMKISRFERHLYNSAAFKARTKARSKCRDKRADVGEFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LTF8</accession>
    <name>A0A8C4LTF8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Mitochondrial inner membrane protein Mpv17</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Protein Mpv17</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">MPV17</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LTF8"/>
    <dbReference type="Ensembl" id="ENSEAST00005014460.2">
        <property type="protein sequence ID" value="ENSEASP00005013314.2"/>
        <property type="gene ID" value="ENSEASG00005009270.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160891"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015267">
        <property type="term" value="F:channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042592">
        <property type="term" value="P:homeostatic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901858">
        <property type="term" value="P:regulation of mitochondrial DNA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF17">
        <property type="entry name" value="PROTEIN MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005013314.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="237" mass="25825" checksum="9175FDAD384DF0D3" modified="2023-09-13" version="2">MALWRAYQRALTAHPWKVQVLTAGSLMGLGDVISQQLVERRGLWEHQTSRTLTMFSLGCGFVGPVVGGWYRVLDRLIPGTTKVDALKKMLLDQGGFAPCFLGCFLPLVGAVNGLSAQDNWAKLQRDYPDALITNYYVRADTSTACFPASLNPDVPRDWGPPDIEVPQLGLLSCSQCGHILRKALTRLFNLFLSTEVGVRPDKAVGCCPVCCCYLELLPVLEGTSALSLPLSVVSALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LTT5</accession>
    <name>A0A8C4LTT5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">40S ribosomal protein S30</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eS30 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LTT5"/>
    <dbReference type="Ensembl" id="ENSEAST00005017636.2">
        <property type="protein sequence ID" value="ENSEASP00005016231.2"/>
        <property type="gene ID" value="ENSEASG00005011272.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01040000240876"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006846">
        <property type="entry name" value="Ribosomal_eS30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12650">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S30/UBIQUITIN-LIKE PROTEIN FUBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12650:SF15">
        <property type="entry name" value="RIBOSOMAL PROTEIN S30, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04758">
        <property type="entry name" value="Ribosomal_S30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="19"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU364011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005016231.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="56" mass="6181" checksum="42B43275550E0D34" modified="2023-09-13" version="2">YLHGSVSGGLVRDQAPKVAKQEKKETGRGKQRMLYKLHFVNVVPTFGKKKGPNANS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LUS2</accession>
    <name>A0A8C4LUS2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Receptor-type tyrosine-protein phosphatase eta</fullName>
            <ecNumber evidence="4">3.1.3.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="21">Protein-tyrosine phosphatase receptor type J</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="27" type="primary">PTPRJ</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Monomer. Interacts with CTNNB1 (phosphorylated) and JUP (phosphorylated). Interacts with FLT3 (phosphorylated). Interacts with GAB1 and GRB2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Ruffle membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the protein-tyrosine phosphatase family. Receptor class 3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="4"/>
    <dbReference type="Ensembl" id="ENSEAST00005018371.2">
        <property type="protein sequence ID" value="ENSEASP00005016913.2"/>
        <property type="gene ID" value="ENSEASG00005011324.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156870"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001772">
        <property type="term" value="C:immunological synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032587">
        <property type="term" value="C:ruffle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045296">
        <property type="term" value="F:cadherin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070097">
        <property type="term" value="F:delta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045295">
        <property type="term" value="F:gamma-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051019">
        <property type="term" value="F:mitogen-activated protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005161">
        <property type="term" value="F:platelet-derived growth factor receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002376">
        <property type="term" value="P:immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030308">
        <property type="term" value="P:negative regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030336">
        <property type="term" value="P:negative regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008285">
        <property type="term" value="P:negative regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042059">
        <property type="term" value="P:negative regulation of epidermal growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051898">
        <property type="term" value="P:negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010642">
        <property type="term" value="P:negative regulation of platelet-derived growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050860">
        <property type="term" value="P:negative regulation of T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043116">
        <property type="term" value="P:negative regulation of vascular permeability"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030220">
        <property type="term" value="P:platelet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048008">
        <property type="term" value="P:platelet-derived growth factor receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050918">
        <property type="term" value="P:positive chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051894">
        <property type="term" value="P:positive regulation of focal adhesion assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050778">
        <property type="term" value="P:positive regulation of immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="CDD" id="cd14615">
        <property type="entry name" value="R-PTPc-J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000009">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000362">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase eta"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001417">
        <property type="entry name" value="Receptor-type tyrosine-protein phosphatase eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000242">
        <property type="entry name" value="PTP_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041201">
        <property type="entry name" value="PTPRJ_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050713">
        <property type="entry name" value="RTP_Phos/Ushers"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957">
        <property type="entry name" value="CYTOKINE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46957:SF5">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18861">
        <property type="entry name" value="PTP_tm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00102">
        <property type="entry name" value="Y_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00700">
        <property type="entry name" value="PRTYPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00194">
        <property type="entry name" value="PTPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50055">
        <property type="entry name" value="TYR_PHOSPHATASE_PTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="7 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 23" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="1021"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="26">
        <location>
            <begin position="414"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="26">
        <location>
            <begin position="672"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="26">
        <location>
            <begin position="766"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="24">
        <location>
            <begin position="1086"/>
            <end position="1343"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="25">
        <location>
            <begin position="1261"/>
            <end position="1334"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="114"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="22">
        <location>
            <begin position="336"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="22">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="22">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="22">
        <location>
            <begin position="336"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00064171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00071459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00081746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005016913.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1382" mass="151679" checksum="A785E4EC52D8A862" modified="2023-09-13" version="2">MSPGKPGAGGAGTRRTGWRRRRRRRRPEAATRAPELGHTAGPGSRVPGTFQGARGMKPAAREARPPPRSPGLRWALPPLLLLLRLGQIVCAGDLTSAAIEVKGTEAPGHFSTVTVTSPSGQSQWNSQGDGAGKTSSEGLTPGTSYPVSFSDASSTCCQVQTEPSPTSVTEAVSIGPSSETSTWKNNNITASNNGELLTTKSETENSPMTLVASPTGDNITDFSPTTPHPHSITTELVSKPNGEPADRAVITDPHPVSDLCVAFVGVTQVTLTWRNGNDTATCRMLLELTGSPKEMTQDLAVNISDLKPGIQYKVTLYFPESNEAQRDLLVTESSLGTNTTDSYSQPPRSPSALVINDRPESSNSSSDGRVTRDTEVLLLGLKSDTQYNATVYCRAEGGTEGQPQTIKFKTNSSQVFDIQVVNISATNMTLTWKINDNESSSVYMYEIQVATETSVLNVTVNETRALISQLSPSTLYNITVRPFLGNGSKGMPGFLQVYTPPGPVSDFRVTSVSMREIGLAWSKNDSDSFEIHTTWVPAGTHSVETTTNQSIVIGGLYPGTEYCFEIFPQGPNGTKGDSQTICDRTAPGAVFDIRVIHVNTTEMQLEWRNTDNASEYIYHLVVQSENGSCERNSSYRAVTLRDLSPGTLYNITICPEVNHVRGISNFTEQYTRPSTVSDIEISTNTTAATLKWQNSDEASPRYTYRLLFGKDGNSSHTAVTVTGVTSATVTGLIPGSSYTVEIFTQVGDVTESLAPGSQSFCTDPAPVASFHCEVVPKEPTLVLKWACPPGTNEGFRLEISCEAWQNLTYRKSCSSENNTEYRTEVTYLNFSTSYNISITALSCNKTAPPSQSMCLTGITDPPSPDGSPNITSVSHNSVKVKFSGFEASHGPIKAYALVLTTGEAVHPSTDVLRYTYEDFKKGDSDTYVTYLITTEGKRRSQGLSEVLKYEVDVGNESTTHGYYNGKLEPLGSYRACVAGFTNIIFHSSNDGLIDGAKSYVSFGRCSDAVFLPQDPGVICGAVFGCIFGALVIVAVGGFIFWRKKRKDAKNNELSFSQIKPRKSKLIKVENFEAYFKKQQADSNCGFAEEYEDLKIVGINLPKYAAELAENRGKNRYNNVLPYDISRVKLSIQTHSTDDYINANYMPGYHSKRDFIATQGPLPNTLKDFWRMVWEKNIYAIVMLTKCVEQGRTKCEEYWPSKQAQDYGDITVAMTSESVLPEWTIRDFTVKNIQTSESHPLRQFHFTSWPDHGVPDTTDLLINFRYLVRDYMKQSPPESPVLVHCSAGVGRTGTFIAIDRLIYQIENENSVDVYGIVSDLRMHRPLMVQTEDQYVFLNQCVLDIIRSQRDSKVDLIYQNTNAMTIYENIAPVSTFGKTNGYIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LV59</accession>
    <name>A0A8C4LV59_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">HPS4 biosis of lysosomal organelles complex 3 subunit 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">HPS4</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LV59"/>
    <dbReference type="Ensembl" id="ENSEAST00005015202.2">
        <property type="protein sequence ID" value="ENSEASP00005013994.2"/>
        <property type="gene ID" value="ENSEASG00005009754.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106841703"/>
    <dbReference type="KEGG" id="eai:106841703"/>
    <dbReference type="CTD" id="89781"/>
    <dbReference type="GeneTree" id="ENSGT00390000007349"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031085">
        <property type="term" value="C:BLOC-3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042827">
        <property type="term" value="C:platelet dense granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007596">
        <property type="term" value="P:blood coagulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007040">
        <property type="term" value="P:lysosome organization"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030318">
        <property type="term" value="P:melanocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903232">
        <property type="term" value="P:melanosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043987">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043989">
        <property type="entry name" value="CCZ1/INTU/HSP4_longin_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026091">
        <property type="entry name" value="HPS4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14407:SF9">
        <property type="entry name" value="BLOC-3 COMPLEX MEMBER HPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14407">
        <property type="entry name" value="HERMANSKY-PUDLAK SYNDROME 4 PROTEIN LIGHT-EAR PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19031">
        <property type="entry name" value="Intu_longin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19033">
        <property type="entry name" value="Intu_longin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCZ1/INTU/HSP4 first Longin" evidence="2">
        <location>
            <begin position="15"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="CCZ1/INTU/HPS4 third Longin" evidence="3">
        <location>
            <begin position="591"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005013994.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="700" mass="75748" checksum="AC406DFB79B089A4" modified="2023-09-13" version="2">MAASSSAETKSASWWNYFFLYDGSKVKEEGDPTRAGICYFYPSQTLLDQQELLCGQIAGVVHCISDISGSPPTLIRLRKLKFAVKVDGDYLWVLGCTVELPDVSCTRFLDQLIGFFHFYNGPVSLAYKSCSREELSTEWDTFIEQILKNTSDLHKIFNSLWNLDQTKVEPLLLLKAALILQTCQRSPHILAGCILYQGLIVSTQLPPSLTAKVLLRRAALRDQRTPAGGEALQDRGAALPLNVQIIPVFLSEEEAVSLREFPGEPITSSPAPPARLQERASALKEGATRHVESTAWTVATTLESTSPDVAWPNGRGENGHVSDHPAELLDTAWGRAPGLSHSLGEQAALFRETEDLDLSEIHIPQDQEMGTSSDYFAFQCVCAPEGGGPHCKESVSDSGNPDPRLPETVPVGTAISGLFSPSAPEMLSQNGALEQCKDLPGDSSAAPISREDCLSRRTSWPLSSPRLESRQTGAELPVGDQGMEQGVDGVHESCSAPHLERGSGSADSQGTRPPADRTDCGSTPASRTGLVRMNLYTHSVKGLVLSLLAEEPLLGDSAAVEEVYHSSLASLNGLEVHLKETLPGDEAASTSRTYSFAHLDRIQNVLMANLPQVATPQDRRFLQAVSLLHSDFAQLPALYEMTVRNASAAVYACCNPAQETYFQQLAAAARSSGFPSPQDSAFSLPGRARQKLLKHGVNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LZF4</accession>
    <name>A0A8C4LZF4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Left-right determination factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106844730</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 12">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LZF4"/>
    <dbReference type="Ensembl" id="ENSEAST00005021079.2">
        <property type="protein sequence ID" value="ENSEASP00005019429.2"/>
        <property type="gene ID" value="ENSEASG00005025761.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010056"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 30"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005160">
        <property type="term" value="F:transforming growth factor beta receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009948">
        <property type="term" value="P:anterior/posterior axis specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13758">
        <property type="entry name" value="TGF_beta_LEFTY1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000028">
        <property type="entry name" value="Left-right determination factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000015">
        <property type="entry name" value="Left-right determination factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003942">
        <property type="entry name" value="LRDF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF226">
        <property type="entry name" value="LEFT-RIGHT DETERMINATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037402">
        <property type="entry name" value="TGFb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01427">
        <property type="entry name" value="TGFBETA4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="4 11" id="KW-0202">Cytokine</keyword>
    <keyword evidence="3 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11 12" id="KW-0339">Growth factor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040385571" description="Left-right determination factor" evidence="13">
        <location>
            <begin position="22"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="14">
        <location>
            <begin position="243"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR037402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005019429.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="367" mass="40795" checksum="31866463143137BC" modified="2023-09-13" version="2">MQPVWLCWALWALPLTGPGAALTEEQILGSLLQQLHLSEVPALDQGDVGQLVIPAHVRAQYVALLQHSHGTHSRGKRFSQNFREVAGRLLGSDASRHLLVFAMEQRLHPDRELLQAVLRLFQEPVPEAALRTHGRLSPRSHRARVTVEWLHVRDDGSNRTSLIDSRLVSVHESGWKAFDVTEAVNFWRQLGRPRQPLLLQVSVQREQPGPRAASAHRLVRFASQGPAGGGRGEPQLELHSLDLGRYGAQGDCDPEAPVTEGTRCCRREMYIDLEGLKWAENWVLEPPGFLAYECVGTCRQPPEPLSFKWPFLGPRQCIASETTSLPVIVSVKEGGRPRAQVVSLPDMRVQKCSCASDGAPVPRKLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LZF6</accession>
    <name>A0A8C4LZF6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cullin-5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CUL5</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10 11">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LZF6"/>
    <dbReference type="Ensembl" id="ENSEAST00005017196.2">
        <property type="protein sequence ID" value="ENSEASP00005015813.2"/>
        <property type="gene ID" value="ENSEASG00005010757.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156648"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031466">
        <property type="term" value="C:Cul5-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090734">
        <property type="term" value="C:site of DNA damage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160072">
        <property type="term" value="F:ubiquitin ligase complex scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140896">
        <property type="term" value="P:cGAS/STING signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001837">
        <property type="term" value="P:epithelial to mesenchymal transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038162">
        <property type="term" value="P:erythropoietin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060397">
        <property type="term" value="P:growth hormone receptor signaling pathway via JAK-STAT"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160049">
        <property type="term" value="P:negative regulation of cGAS/STING signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010719">
        <property type="term" value="P:negative regulation of epithelial to mesenchymal transition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051895">
        <property type="term" value="P:negative regulation of focal adhesion assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120184">
        <property type="term" value="P:negative regulation of focal adhesion disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060400">
        <property type="term" value="P:negative regulation of growth hormone receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051894">
        <property type="term" value="P:positive regulation of focal adhesion assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070979">
        <property type="term" value="P:protein K11-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070936">
        <property type="term" value="P:protein K48-linked ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000142">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000009">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000014">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000017">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.130:FF:000004">
        <property type="entry name" value="Cullin 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016157">
        <property type="entry name" value="Cullin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01256">
        <property type="entry name" value="CULLIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Cullin family profile" evidence="12">
        <location>
            <begin position="403"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00040451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005015813.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="780" mass="90989" checksum="71E7B2B3FF0CB241" modified="2023-09-13" version="2">MATSNRSENKGSLQFEDKWDFMRPIVLKLLRQECVTKQQWFDLFSDVHAVCLWDDKGPAKIHQALKEDILEFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEITLMGKQGSNKKSNVEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLLYEPQVNSPKDFTEGTLFSVNQEFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQIEWLIEHKYIRRDESDINTFIYMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M2E6</accession>
    <name>A0A8C4M2E6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Cystinosin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">CTNS</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Cystine/H(+) symporter that mediates export of cystine, the oxidized dimer of cysteine, from lysosomes. Plays an important role in melanin synthesis by catalyzing cystine export from melanosomes, possibly by inhibiting pheomelanin synthesis. In addition to cystine export, also acts as a positive regulator of mTORC1 signaling in kidney proximal tubular cells, via interactions with components of the v-ATPase and Ragulator complexes. Also involved in small GTPase-regulated vesicle trafficking and lysosomal localization of LAMP2A, independently of cystine transporter activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-cystine(out) + H(+)(out) = L-cystine(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:66172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:35491"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:66173"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="17">Interacts with components of the V-ATPase complex. Interacts with components of the Ragulator complex. Interacts with RRAGA/RagA and RRAGC/RagC. Interacts with AP-3 complex subunit mu (AP3M1 or AP3M2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="16">Melanosome membrane</location>
            <topology evidence="16">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cystinosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070338674.1">
        <property type="nucleotide sequence ID" value="XM_070482573.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070338675.1">
        <property type="nucleotide sequence ID" value="XM_070482574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M2E6"/>
    <dbReference type="Ensembl" id="ENSEAST00005021022.2">
        <property type="protein sequence ID" value="ENSEASP00005019373.1"/>
        <property type="gene ID" value="ENSEASG00005013325.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106825663"/>
    <dbReference type="CTD" id="1497"/>
    <dbReference type="GeneTree" id="ENSGT00390000005338"/>
    <dbReference type="OMA" id="WIDVIYT"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033162">
        <property type="term" value="C:melanosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015184">
        <property type="term" value="F:L-cystine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015295">
        <property type="term" value="F:solute:proton symporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007628">
        <property type="term" value="P:adult walking behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046034">
        <property type="term" value="P:ATP metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007420">
        <property type="term" value="P:brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007625">
        <property type="term" value="P:grooming behavior"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002088">
        <property type="term" value="P:lens development in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007616">
        <property type="term" value="P:long-term memory"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042438">
        <property type="term" value="P:melanin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048021">
        <property type="term" value="P:regulation of melanin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008542">
        <property type="term" value="P:visual learning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000016">
        <property type="entry name" value="Cystinosin homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.290:FF:000015">
        <property type="entry name" value="cystinosin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005282">
        <property type="entry name" value="LC_transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00951">
        <property type="entry name" value="2A43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13131:SF5">
        <property type="entry name" value="CYSTINOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0458">Lysosome</keyword>
    <keyword evidence="10" id="KW-0470">Melanin biosynthesis</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 20" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0769">Symport</keyword>
    <keyword evidence="4 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="20">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034095041" description="Cystinosin" evidence="20">
        <location>
            <begin position="23"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="162"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="262"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="302"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00060313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00060435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00066203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005019373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="397" mass="44939" checksum="86299E7AE261F370" modified="2022-01-19" version="1">MIRRWLIIFILSLLKLIGKCESTVSLTVPPTVKLENGSSANVSIILQPPLNATLVITFEITFRSKNITILELPDEVVVPPGVTHSSFQVTPQNVGQVTVFLHGNHSNQTGPRIRFLVIHSNVVSIVDQVIGWIYFVAWSISFYPQVITNWRRKSVVGLSFDFVSLNLTGFVAYSVFNIGLLWVPYFKEQFFLKYPNGVNPVDANDVFFSLHAVTLTLVVLVQCCLYERGNQRVSWPAIGFLVLSWLFALITMILAAVGATTWLQFLFCFSYIKLAVTLVKYFPQAYMNFHYKSTEGWSIGNVLLDFTGGSFSLLQMFLQSYNNDQWTLIFGDPTKFGLGIFSIFFDIVFFIQHFCLYRKKPGLQAAHTARPRQDWALSLEPKALPQATSVSRSSLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M2R5</accession>
    <name>A0A8C4M2R5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Elongation of very long chain fatty acids protein 7</fullName>
            <ecNumber evidence="10">2.3.1.199</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">3-keto acyl-CoA synthase ELOVL7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">ELOVL fatty acid elongase 7</fullName>
            <shortName evidence="10">ELOVL FA elongase 7</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Very long chain 3-ketoacyl-CoA synthase 7</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Very long chain 3-oxoacyl-CoA synthase 7</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">ELOVL7</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme with higher activity toward C18 acyl-CoAs, especially C18:3(n-3) acyl-CoAs and C18:3(n-6)-CoAs. Also active toward C20:4-, C18:0-, C18:1-, C18:2- and C16:0-CoAs, and weakly toward C20:0-CoA. Little or no activity toward C22:0-, C24:0-, or C26:0-CoAs. May participate to the production of saturated and polyunsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>a very-long-chain acyl-CoA + malonyl-CoA + H(+) = a very-long-chain 3-oxoacyl-CoA + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:32727"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:90725"/>
            <dbReference type="ChEBI" id="CHEBI:90736"/>
            <dbReference type="EC" id="2.3.1.199"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">Lipid metabolism; fatty acid biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Endoplasmic reticulum membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The C-terminal di-lysine motif may confer endoplasmic reticulum localization.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ELO family. ELOVL7 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.199" evidence="10"/>
    <dbReference type="RefSeq" id="XP_014686523.1">
        <property type="nucleotide sequence ID" value="XM_014831037.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044627994.1">
        <property type="nucleotide sequence ID" value="XM_044772059.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M2R5"/>
    <dbReference type="Ensembl" id="ENSEAST00005018613.2">
        <property type="protein sequence ID" value="ENSEASP00005017137.1"/>
        <property type="gene ID" value="ENSEASG00005011863.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106824625"/>
    <dbReference type="KEGG" id="eai:106824625"/>
    <dbReference type="CTD" id="79993"/>
    <dbReference type="GeneTree" id="ENSGT01050000244838"/>
    <dbReference type="OMA" id="GWANGYS"/>
    <dbReference type="UniPathway" id="UPA00094"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009922">
        <property type="term" value="F:fatty acid elongase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034625">
        <property type="term" value="P:fatty acid elongation, monounsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034626">
        <property type="term" value="P:fatty acid elongation, polyunsaturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019367">
        <property type="term" value="P:fatty acid elongation, saturated fatty acid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035338">
        <property type="term" value="P:long-chain fatty-acyl-CoA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030148">
        <property type="term" value="P:sphingolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042761">
        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03207">
        <property type="entry name" value="VLCF_elongase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030457">
        <property type="entry name" value="ELO_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002076">
        <property type="entry name" value="ELO_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033670">
        <property type="entry name" value="ELOVL7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157:SF118">
        <property type="entry name" value="ELONGATION OF VERY LONG CHAIN FATTY ACIDS PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11157">
        <property type="entry name" value="FATTY ACID ACYL TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01151">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01188">
        <property type="entry name" value="ELO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10 11" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="5 10 11" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2 10 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 10 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="28"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="69"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="118"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="206"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10 11">
        <location>
            <begin position="233"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Di-lysine motif" evidence="10">
        <location>
            <begin position="277"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005017137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="281" mass="33603" checksum="C91486A6FCF55BEA" modified="2022-01-19" version="1">MAFSDLTSRTMRLYDNWIKHADPRVEDWLLMSSPLPQTIILVLYVYFVTSLGPKLMENRKPFDLKKVMITYNFFIVLFSVYLCYEFVMSGWGTGYSLRCEIVDYSWSPTALRMARTCWLYYFSKFIELLDTIFFVLRKKNSQVTFLHVFHHTIMPWTWWFGVKFAAGGLGTFHAFLNTAVHVVMYSYYGLCALGPAYQQYLWWKKYLTSLQLVQFLIVTIHISQFFFMEDCKYQFPVFLYIIMSYGCIFLLLFLHFWYRAYTKGQRLPKTMKNGICKNKDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M4D6</accession>
    <name>A0A8C4M4D6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BOD1/SHG1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">BOD1</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BOD1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4M4D6"/>
    <dbReference type="Ensembl" id="ENSEAST00005018734.2">
        <property type="protein sequence ID" value="ENSEASP00005017244.2"/>
        <property type="gene ID" value="ENSEASG00005011948.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154979"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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            <end position="123"/>
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                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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</entry>
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                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
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        <property type="entry name" value="CTDL_fold"/>
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        <property type="entry name" value="Ly49_N"/>
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        <property type="entry name" value="Mouse_KLRs"/>
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        <property type="entry name" value="NKR-like_CTLD"/>
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    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0430">Lectin</keyword>
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    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="47"/>
            <end position="70"/>
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    <feature type="domain" description="C-type lectin" evidence="13">
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            <begin position="160"/>
            <end position="270"/>
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            <end position="36"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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            <dbReference type="ARBA" id="ARBA00023180"/>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="282" mass="32871" checksum="0A106610C5455291" modified="2023-09-13" version="2">MSNQEVTYCSLRFLQSPSESQNTLRPGGTQRPGKTDDKEFSVPWHPFVVTLGILCLLLLVTVTVLGTMIFQCFKEKHQQEEILRNLIPKYDSIRNNNYSKEQLLINKTLECDILKNKTLHQKKELDSLFMEKMRCHIKQEIFSKSLQSTGKFNEDHWSCCGVNCYYFTSENENWMGCKETCQRYNLSLLKIDDEDELNFVQRQTYRDSYWIGLSYNATESKWKWIDSGMSSGINFKIMSLPSGRRGKCAFLSAIRIADIDCAKKYKCICEKRTDCDFTACFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M7P9</accession>
    <name>A0A8C4M7P9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Triacylglycerol lipase</fullName>
            <ecNumber evidence="14">3.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Pancreatic lipase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">PNLIPRP1</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a triacylglycerol + H2O = a diacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12044"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17855"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="EC" id="3.1.1.3"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the AB hydrolase superfamily. Lipase family.</text>
    </comment>
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    <dbReference type="Ensembl" id="ENSEAST00005023117.2">
        <property type="protein sequence ID" value="ENSEASP00005021293.2"/>
        <property type="gene ID" value="ENSEASG00005014579.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162375"/>
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        <property type="component" value="Chromosome 2"/>
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    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004465">
        <property type="term" value="F:lipoprotein lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00707">
        <property type="entry name" value="Pancreat_lipase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01759">
        <property type="entry name" value="PLAT_PL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000033">
        <property type="entry name" value="Pancreatic triacylglycerol lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.60.20:FF:000003">
        <property type="entry name" value="Triacylglycerol lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.60.20">
        <property type="entry name" value="PLAT/LH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013818">
        <property type="entry name" value="Lipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016272">
        <property type="entry name" value="Lipase_LIPH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033906">
        <property type="entry name" value="Lipase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002331">
        <property type="entry name" value="Lipase_panc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001024">
        <property type="entry name" value="PLAT/LH2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036392">
        <property type="entry name" value="PLAT/LH2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000734">
        <property type="entry name" value="TAG_lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610:SF108">
        <property type="entry name" value="INACTIVE PANCREATIC LIPASE-RELATED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11610">
        <property type="entry name" value="LIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00151">
        <property type="entry name" value="Lipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01477">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000865">
        <property type="entry name" value="Lipoprotein_lipase_LIPH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00823">
        <property type="entry name" value="PANCLIPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00821">
        <property type="entry name" value="TAGLIPASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00308">
        <property type="entry name" value="LH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49723">
        <property type="entry name" value="Lipase/lipooxygenase domain (PLAT/LH2 domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50095">
        <property type="entry name" value="PLAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 11 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 14" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="7 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 14" id="KW-0964">Secreted</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040535326" description="Triacylglycerol lipase" evidence="14">
        <location>
            <begin position="18"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="domain" description="PLAT" evidence="15">
        <location>
            <begin position="356"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9">
        <location>
            <position position="194"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="9">
        <location>
            <position position="281"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="21"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="109"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="303"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="317"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11 12">
        <location>
            <begin position="451"/>
            <end position="467"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010701"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000865-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000865-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000865-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU362046"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005021293.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="468" mass="51505" checksum="5C032C8EB048D579" modified="2023-09-13" version="2">MGNIWTIVLFLLGAAKGKEICYDHVGCFSDSEPWAGTAIRPLNVLPWSPERIGTRFLLYTNENPDTFQSLLPSDPSTVEASNFQADKKTRFIIHGFIDKGDESWLLDMCKNMFKVEEVNCICVDWKKGSQTTYTQAANNVRVVGAQVAQMLGMLSVNYSYSPSQVHLIGHSLGAHVAGEAGSRTPGLGRITGLDPVEASFQGTPEEVRLDPSDAVFVDVIHTDAAPLIPFLGFGTKQQMGHLDFFPNGGEEMPGCEKNALSQIVDLDGIWAGTRDFVACNHLRSYKYYSESILSPDGFAAYPCDSYRAFESNKCFPCPDAGCPQMGHYADKFAGKTSEEQQKFFLNTGDSSNFARWRYGISITLSGRTVTGQVKVALFGNKGNTHQYDVFRGIIQPGSTHSSEFDAELDVGTIEKVKFLWNNNVVNPTLPRVGAAKITVQKGEEKTGYSFCSEDTVREDLLLTLLPCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M937</accession>
    <name>A0A8C4M937_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Voltage-dependent R-type calcium channel subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">CACNA1E</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1E gives rise to R-type calcium currents.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>Ca(2+)(in) = Ca(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29671"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 19">Membrane</location>
            <topology evidence="1 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005025659.2">
        <property type="protein sequence ID" value="ENSEASP00005023651.2"/>
        <property type="gene ID" value="ENSEASG00005007400.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155601"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005891">
        <property type="term" value="C:voltage-gated calcium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008331">
        <property type="term" value="F:high voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098703">
        <property type="term" value="P:calcium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007268">
        <property type="term" value="P:chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.350:FF:000001">
        <property type="entry name" value="Voltage-dependent L-type calcium channel subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <dbReference type="FunFam" id="1.10.287.70:FF:000023">
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        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR45628">
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    <dbReference type="SMART" id="SM01062">
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    <keyword evidence="5 19" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="4 19" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="14" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0407">Ion channel</keyword>
    <keyword evidence="12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="13 22" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 22" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 22" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <keyword evidence="10 19" id="KW-0851">Voltage-gated channel</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="22">
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00036634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR602077-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005023651.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="2351" mass="265907" checksum="2ADB0D52C06870FD" modified="2023-09-13" version="2">MKRRRGGLIEQRDIIKAHEAHKMQSTPQARRKEWEMARFGEAVVGRPGSGDGDSDQSRNRQGTPVPASGPAAAYKQSKAQRARTMALYNPIPVRQNCFTVNRSLFIFGEDNIVRKYAKKLIDWPPFEYMILATIIANCIVLALEQHLPEDDKTPMSRRLEKTEPYFIGIFCFEAGIKIVALGFIFHKGSYLRNGWNVMDFIVVLSGILATAGTHFNTHVDLRTLRAVRVLRPLKLVSGIPSLQIVLKSIMKAMVPLLQIGLLLFFAILMFAIIGLEFYSGKLHRACFMNNSGILEGFDPPHPCGVQGCPAGYECRDWIGPNDGITQFDNILFAVLTVFQCITMEGWTTVLYNTNDALGATWNWLYFIPLIIIGSFFVLNLVLGVLSGEFAKERERVENRRAFMKLRRQQQIERELNGYRAWIDKAEEVMLAEENKNAGTSALEVLRRATIKRSRTEAMTRDSSDEHCVDISSVGTPLARASIKSAKVDGASYFRHKERLLRISIRHMVKSQVFYWIVLSLVALNTACVAIVHHNQPQWLTHLLYYAEFLFLGLFLLEMSLKMYGMGPRLYFHSSFNCFDFGVTVGSIFEVVWAIFRPGTSFGISVLRALRLLRIFKITKYWASLRNLVVSLMSSMKSIISLLFLLFLFIVVFALLGMQLFGGRFNFNDGTPSANFDTFPAAIMTVFQILTGEDWNEVMYNGIRSQGGVSSGMWSAIYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAFNQKHALQKAKEVSPMSAPNMPSIERDRRRRHHMSMWEPRSSHLRERRRRHHMSVWEQRTSQLRRHMQMSSQEALNKEEAPPMNPLNPLNPLSPLNPLNAHPSLYRRSRPIEGLALGLGLEKCEEERISRGGSLKGDGGDLSSALDNQRSPLSLGKREPPWLSRPCHGNCDLTQQEAGGGETVVTFEDRARHRQSQRRSRHRRVRTEGKEPTSTSRSRSASQERSLDEGVSTEGEKDCEPRGSHGVKEPTIQEEERARDLRRTNSLMVSRGSGLAGALDEANTPLVLPQPELEVGKDAALTEQEPEGSSEQALLGDVHLDMGRAISQSEPDLSCSTANTDKAATESTSVTVAIPDVGPLVDSTVVHISNKTDGEASPLKEAEIKEEEEEEVEKKQQKKEKRETGKAMVPHSSMFIFSTTNPIRRACHYIVNLRYFEMCILLVIAASSIALAAEDPVLTNSERNKVLRYFDYVFTGVFTFEMVIKMIDQGLILQDGSYFRDLWNILDFVVVVGALVAFALANALGTNKGRDIKTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVTSLKNVFNILIVYKLFMFIFAVIAVQLFKGKFFYCTDSSKDTEKECIGNYVDHEKNKMEVKGREWKRHEFHYDNIIWALLTLFTVSTGEGWPQVLQHSVDVTEEDRGPSRSNRMEMSIFYVVYFVVFPFFFVNIFVALIIITFQEQGDKMMEECSLEKNERACIDFAISAKPLTRYMPQNRHTFQYRVWHFVVSPSFEYTIMAMIALNTIVLMMKYYSAPCTYELALKYLNIAFTMVFSLECVLKIIAFGFLNYFRDTWNIFDFITVIGSITEIILTDSKLVNTSGFNMSFLKLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIKLDEESHINRHNNFRSFFGSLMLLFRSATGEAWQEIMLSCLGEKGCEPDTTAPSGQTENERCGTDLAYVYFVSFIFFCSFLMLNLFVAVIMDNFEYLTRDSSILGPHHLDEFVRVWAEYDRAACGRIHYTEMYEMLTLMSPPLGLGKRCPSKVAYKRLVLMNMPVAEDMTVHFTSTLMALIRTALDIKIAKGGADRQQLDAELQKETLAIWPHLSQKMLDLLVPMPKASDLTVGKIYAAMMIMDYYKQSKVKKQRQQLEEQKNAPMFQRMEPSSLPQEIIANAKALPYLQQDPVSGLSGHSGYPSMSPLSPQEIFQLACMDPADDGQFQEQQSLEPEVSEFKSVQPSNHGIYLPSDTQEHAGSGRASSMPRLTVDPQVVTDPSSMRRSFSTIRDKRSNSSWLEEFSMERSSENTYKSRRRSYHSSLRLSAHRLNSDSGHKSDTHRSGGRERGRSKERKHLLSPDVSRCNSEERGTQADWESPERRQSRSPSEGRSQTPNRQGTGSLSESSIPSVSDTSTPRRSRRQLPPVPPKPRPLLSYSSLIRQHTGSISPPADGSEGGSPLASQALESNDAILTESSNSRHAQQGPHSSPQRYISEPYLALHEDSHASDCGEEETLTFEAAVATSLGRSNTIGSAPPLRHSWQMPNGHYRRRRRGGPGPGVMCGAVSDLLSDTEEDDKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M9S7</accession>
    <name>A0A8C4M9S7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Extracellular calcium-sensing receptor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Parathyroid cell calcium-sensing receptor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">CASR</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="19">Homodimer; disulfide-linked. Interacts with VCP. Interacts with ARRB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 3 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044627432.1">
        <property type="nucleotide sequence ID" value="XM_044771497.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M9S7"/>
    <dbReference type="Ensembl" id="ENSEAST00005024604.2">
        <property type="protein sequence ID" value="ENSEASP00005022676.2"/>
        <property type="gene ID" value="ENSEASG00005015466.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106823746"/>
    <dbReference type="KEGG" id="eai:106823746"/>
    <dbReference type="CTD" id="846"/>
    <dbReference type="GeneTree" id="ENSGT00940000157596"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007193">
        <property type="term" value="P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005513">
        <property type="term" value="P:detection of calcium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900227">
        <property type="term" value="P:positive regulation of NLRP3 inflammasome complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051924">
        <property type="term" value="P:regulation of calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099505">
        <property type="term" value="P:regulation of presynaptic membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd15282">
        <property type="entry name" value="7tmC_CaSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06364">
        <property type="entry name" value="PBP1_CaSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000016">
        <property type="entry name" value="Taste 1 receptor member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.50.30:FF:000002">
        <property type="entry name" value="Vomeronasal 2 receptor, h1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000388">
        <property type="entry name" value="Vomeronasal 2, receptor 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.50.30">
        <property type="entry name" value="GPCR, family 3, nine cysteines domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000337">
        <property type="entry name" value="GPCR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011500">
        <property type="entry name" value="GPCR_3_9-Cys_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038550">
        <property type="entry name" value="GPCR_3_9-Cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017978">
        <property type="entry name" value="GPCR_3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000068">
        <property type="entry name" value="GPCR_3_Ca_sens_rcpt-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017979">
        <property type="entry name" value="GPCR_3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061">
        <property type="entry name" value="CALCIUM-SENSING RECEPTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24061:SF358">
        <property type="entry name" value="EXTRACELLULAR CALCIUM-SENSING RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00003">
        <property type="entry name" value="7tm_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07562">
        <property type="entry name" value="NCD3G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00592">
        <property type="entry name" value="CASENSINGR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00248">
        <property type="entry name" value="GPCRMGR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00979">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00980">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00981">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50259">
        <property type="entry name" value="G_PROTEIN_RECEP_F3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-0675">Receptor</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 22" id="KW-0732">Signal</keyword>
    <keyword evidence="16" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0832">Ubl conjugation</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040219868" description="Extracellular calcium-sensing receptor" evidence="22">
        <location>
            <begin position="20"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="612"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="649"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="682"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="725"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="770"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="804"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="832"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 3 profile" evidence="23">
        <location>
            <begin position="612"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
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    <sequence length="1084" mass="121468" checksum="85CE9DE395050069" modified="2023-09-13" version="2">MAFYSCCLILVAFTWCTSAYGPDQRAQKKGDIILGGLFPIHFGVAAKDQDLESRPESVECIRYNFRGFRWLQAMIFAIEEINSSPALLPNMTLGYRIFDTCNTVSKALEATLSFVAQNKIDSLNLDEFCNCSEHIPSTIAVVGATGSGVSTAVANLLGLFYIPQVSYASSSRLLSNKNQFKSFLRTIPNDEHQATAMADIIEYFRWNWVGTIAADDDYGRPGIEKFREEAEERDICIDFSELISQYSDEEEIQHVVEVIQNSTAKVIVVFSSGPDLEPLIKEIVRRNITGKIWLASEAWASSSLIAMPEYFHVVGGTIGFALRAGQIPGFREFLQKVHPKKSAHNGFAKEFWEETFNCHLQEGAKGPLPMDTFLRGHEEAGGRLSNSSTAFRPLCTGDENISSVETPYMDYTHLRISYNVYLAVYSIAHALQDIYTCSPGRGLFTNGSCADIKKVEAWQVLKHLRHLNFTNNMGEQVTFDECGDLVGNYSIINWHLSPEDGSIVFKEVGYYNVYAKKGERLFINEKKILWSGFSREVPFSNCSRDCLAGTRKGIIEGEPTCCFECVECPDGEYSDETDASACDKCPDDFWSNENHTSCIAKEIEFLSWTEPFGIALTLFAVLGIFLTGFVLGVFIKFRNTPIVKATNRELSYLLLFSLLCCFSSSLFFIGEPQDWTCRLRQPAFGISFVLCISCILVKTNRVLLVFEAKIPTSFHRKWWGLNLQFLLVFLCTFMQIVICVIWLYTAPPSSYRNHELEDEIIFITCHEGSLMALGFLIGYTCLLAAICFFFAFKSRKLPENFNEAKFITFSMLIFFIVWISFIPAYASTYGKFVSAVEVIAILAASFGLLACIFFNKVYIILFKPSRNTIEEVRCSTAAHAFKVAARATLRRSNVSRKRSSSLGGSTGSTPSSSISSKSNSEDPFPQPERQKQQQPLALTQQEQQQQQQQPQQPLTLQQQQQAQQQPRCKQKVIFGSGTVTFSLSFDEPQKSAMAHRNSTHQNSLESQKSNDTLTRHQALLPLQRGEIDSELSTQETGLQGPAVGDRQPERKNPEEMSPTLVVSNSRSFVISGGGSTVTENILHS</sequence>
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    <name>A0A8C4MAI5_EQUAS</name>
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        <submittedName>
            <fullName evidence="6">Intermediate filament family orphan 1</fullName>
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        <name evidence="6" type="primary">IFFO1</name>
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        <name type="scientific">Equus asinus</name>
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                <person name="Guo"/>
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                <person name="Sun"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
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                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Yu"/>
                <person name="J."/>
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                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="entry name" value="NON-HOMOLOGOUS END JOINING FACTOR IFFO1"/>
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    <keyword evidence="1" id="KW-0403">Intermediate filament</keyword>
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            <dbReference type="ARBA" id="ARBA00023054"/>
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    </evidence>
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</entry>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Liu"/>
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                <person name="Wang"/>
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                <person name="Guo"/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the DSH family.</text>
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    <dbReference type="KEGG" id="eai:106834527"/>
    <dbReference type="CTD" id="1857"/>
    <dbReference type="GeneTree" id="ENSGT00950000182903"/>
    <dbReference type="OMA" id="RFEEFHL"/>
    <dbReference type="OrthoDB" id="10031689at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002020">
        <property type="term" value="F:protease binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032880">
        <property type="term" value="P:regulation of protein localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060071">
        <property type="term" value="P:Wnt signaling pathway, planar cell polarity pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd04438">
        <property type="entry name" value="DEP_dishevelled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06717">
        <property type="entry name" value="PDZ_Dishevelled-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.130:FF:000001">
        <property type="entry name" value="Segment polarity protein dishevelled homolog DVL-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000040">
        <property type="entry name" value="segment polarity protein dishevelled homolog DVL-3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Segment polarity protein dishevelled homolog DVL-3"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000591">
        <property type="entry name" value="DEP_dom"/>
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    <dbReference type="InterPro" id="IPR024580">
        <property type="entry name" value="Dishevelled_C-dom"/>
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    <dbReference type="InterPro" id="IPR008339">
        <property type="entry name" value="Dishevelled_fam"/>
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    <dbReference type="InterPro" id="IPR003351">
        <property type="entry name" value="Dishevelled_protein_dom"/>
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    <dbReference type="InterPro" id="IPR001158">
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    <dbReference type="InterPro" id="IPR038207">
        <property type="entry name" value="DIX_dom_sf"/>
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    <dbReference type="InterPro" id="IPR015506">
        <property type="entry name" value="Dsh/Dvl-rel"/>
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    <dbReference type="InterPro" id="IPR008342">
        <property type="entry name" value="DVL3"/>
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    <dbReference type="InterPro" id="IPR001478">
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    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
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    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
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    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10878">
        <property type="entry name" value="SEGMENT POLARITY PROTEIN DISHEVELLED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10878:SF6">
        <property type="entry name" value="SEGMENT POLARITY PROTEIN DISHEVELLED HOMOLOG DVL-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00610">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02377">
        <property type="entry name" value="Dishevelled"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00778">
        <property type="entry name" value="DIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12316">
        <property type="entry name" value="Dsh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01760">
        <property type="entry name" value="DISHEVELLED"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01763">
        <property type="entry name" value="DISHEVELLED3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00021">
        <property type="entry name" value="DAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00049">
        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DEP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="DIX" evidence="10">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="PDZ" evidence="8">
        <location>
            <begin position="249"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="DEP" evidence="9">
        <location>
            <begin position="422"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="85"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="546"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="175"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="199"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="213"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="565"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="582"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="604"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="653"/>
            <end position="682"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008735"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="718" mass="78357" checksum="DD542DF78D9C9AD0" modified="2022-01-19" version="1">MGETKIIYHLDGQETPYLVKLPLPAERVTLADFKGVLQRPSYKFFFKSMDDDFGVVKEEISDDNAKLPCFNGRVVSWLVSAEGSHPEPAPFCADNPSELPPPMERTGGIGDSRPPSFHPHAGGGSQENLDNDTETDSLVSAQRERPRRRDGPEHTTRLNGTAKGERRREPGGYDSSSTLMSSELETTSFFDSDEDDSTSRFSSSTEQSSASRLMRRHKRRRRKQKVSRIERSSSFSSITDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNEINFENMSNDDAVRVLREIVHKPGPITLTVAKCWDPSPRGCFTLPRSEPIRPIDPAAWVSHTAAMTGTFPAYGMSPSLSTITSTSSSITSSIPDTERLDDFHLSIHSDMAAIVKAMASPESGLEVRDRMWLKITIPNAFIGSDVVDWLYHNVEGFTDRREARKYASNLLKAGFIRHTVNKITFSEQCYYIFGDLCGNMANLSLHDHDGSSGASDQDTLAPLPHPGAAPWPMAFPYQYPPPPHPYNPHPGFPELGYSYGGGSASSQHSEGSRSSGSNRSGSDRRKEKDPKTGDSKSGGSGSESDHTTRSSLRGPRERAPSERSGPAASEHSHRSHHSLASSLRSHHTHPSYGPPGVPPLYGPPMLMMPPPPAAMGPPGAPPGRDLASVPPELTASRQSFRMAMGNPTKNFGLFDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MCI0</accession>
    <name>A0A8C4MCI0_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">RAS and EF-hand domain containing</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">RASEF</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Binds predominantly GDP, and also GTP. Acts as a dynein adapter protein that activates dynein-mediated transport and dynein-dynactin motility on microtubules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. Interacts with the dynein-dynactin complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the small GTPase superfamily. Rab family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014721983.1">
        <property type="nucleotide sequence ID" value="XM_014866497.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MCI0"/>
    <dbReference type="Ensembl" id="ENSEAST00005025499.2">
        <property type="protein sequence ID" value="ENSEASP00005023500.2"/>
        <property type="gene ID" value="ENSEASG00005015967.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106847290"/>
    <dbReference type="KEGG" id="eai:106847290"/>
    <dbReference type="CTD" id="158158"/>
    <dbReference type="GeneTree" id="ENSGT00940000159488"/>
    <dbReference type="OrthoDB" id="9879408at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00154">
        <property type="entry name" value="Rab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000217">
        <property type="entry name" value="RAS and EF-hand domain containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1490:FF:000019">
        <property type="entry name" value="RAS and EF-hand domain containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001348">
        <property type="entry name" value="Ras and EF-hand domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR050227">
        <property type="entry name" value="Rab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47977">
        <property type="entry name" value="RAS-RELATED PROTEIN RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="8 12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EF-hand" evidence="14">
        <location>
            <begin position="42"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="78"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="375"/>
            <end position="405"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="457"/>
            <end position="481"/>
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    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="191"/>
            <end position="352"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
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            <begin position="375"/>
            <end position="388"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="459"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006270"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
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    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00065295"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005023500.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="740" mass="82618" checksum="289C33B1F63AF549" modified="2023-09-13" version="2">MEADGDREELARLRSLFAACDANRSGRLEREEFGALCAELRVRPADAEAVFQRLDADRDGTITFQEFARGFRGALRAGRSGGWGSPEPASAAARAGLHPGDSVEDEGDEDAAEALAAPWGPASPGLAWQDFQARLGDEAKFIPRKEQVSTLYQNINLVEPRLIQPYEHVIKNFIREIRLQSTEMENLAIAVKRAQDKAAIQLSELEEEMDQRIQAAEHKTRKDEKRKAEEALTDLRRQYETEVGDLQVTIKKLKKLEEQSKHVSRKEDVAALKKQIYDLSMENQRVKKDLLEAQTSIAFLQSELDALKSDYADQSLNSERDLEIIREYTEDRNSLERQIEMLQKANRKLHDSNDGLRSALENSYSKFNRSLRINNISPGNTLSRSSPKFNRHSPQPLGYDRSPRSSFVDEDCDSLALCDPMQRMNCEVDSLPESCFDSGLSTLRDSNEYDSEVECKQQRGFQRSHGTQESLGGDASDTDVPEIRDEETYGSEGVASVLDWNPQGSVSEGSTVGSSRKPIPALALQTDVVDDNYKSSSSQKAYKIVLAGDAAVGKSSFLMRLCKNEFRGNTSATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRRADGVLLLYDVTCEKSFLNVREWVDMIEDATHDPIPIMLVGNKADLRDTAAAEGQKCVPGYCGEKLAMTHGALFCETSAKDGSNIVEAVLHLAREVKKRIDEDDSKSITNLTGTNSKKTIQMKNCCNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MGE1</accession>
    <name>A0A8C4MGE1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Cell membrane</location>
            <topology evidence="2 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MGE1"/>
    <dbReference type="Ensembl" id="ENSEAST00005027779.2">
        <property type="protein sequence ID" value="ENSEASP00005025585.2"/>
        <property type="gene ID" value="ENSEASG00005017438.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000164344"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15913">
        <property type="entry name" value="7tmA_OR11G-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF369">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="102"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="208"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="276"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="45"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025585.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="311" mass="35037" checksum="BF28D85C06812F33" modified="2023-09-13" version="2">MKSPGGNNHSDPVTEFILLGFPCTWETQILLFSLFSVIYFLTVMGNLCIICTVWCVHHLHTPMYILLANFSFLEIWYVTSTVPNMLANFLSERNSISFSGCFLQFYFFFSMGTTETLFLSAMAFDRYLAICRPLHYPTVMTVQRCIRMGAYCWICGFSCFLLPVYLISQLPFCGPNTIDHFLCPLLALASACIPLPGTVLICGTTSSLLIFATFFYSIGSYTLVLRAVIQVPSVAGRKKAFSTCTSHLAIVFPFYGSVMMTYVIPRSGQEEDMQKLTTLFYSVLTPLFNSMIYRLQNKEMKNALKKVLGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MH81</accession>
    <name>A0A8C4MH81_EQUAS</name>
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        <submittedName>
            <fullName evidence="16">Potassium sodium-activated channel subfamily T member 1</fullName>
        </submittedName>
    </protein>
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        <name evidence="16" type="primary">KCNT1</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MH81"/>
    <dbReference type="Ensembl" id="ENSEAST00005028975.2">
        <property type="protein sequence ID" value="ENSEASP00005026684.2"/>
        <property type="gene ID" value="ENSEASG00005017622.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156880"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005228">
        <property type="term" value="F:intracellular sodium-activated potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015271">
        <property type="term" value="F:outward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051289">
        <property type="term" value="P:protein homotetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000011">
        <property type="entry name" value="Potassium channel subfamily T member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000034">
        <property type="entry name" value="Potassium channel subfamily T member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000069">
        <property type="entry name" value="Potassium sodium-activated channel subfamily T member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003929">
        <property type="entry name" value="K_chnl_BK_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047871">
        <property type="entry name" value="K_chnl_Slo-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027">
        <property type="entry name" value="CALCIUM-ACTIVATED POTASSIUM CHANNEL ALPHA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10027:SF14">
        <property type="entry name" value="POTASSIUM CHANNEL SUBFAMILY T MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03493">
        <property type="entry name" value="BK_channel_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22614">
        <property type="entry name" value="Slo-like_RCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="11" id="KW-0407">Ion channel</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0630">Potassium</keyword>
    <keyword evidence="6" id="KW-0631">Potassium channel</keyword>
    <keyword evidence="4" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="178"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="211"/>
            <end position="232"/>
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    </feature>
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            <end position="321"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="333"/>
            <end position="358"/>
        </location>
    </feature>
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        <location>
            <begin position="375"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="15">
        <location>
            <begin position="804"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="683"/>
            <end position="712"/>
        </location>
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            <begin position="1078"/>
            <end position="1108"/>
        </location>
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        <location>
            <begin position="1234"/>
            <end position="1261"/>
        </location>
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            <begin position="1087"/>
            <end position="1103"/>
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        <location>
            <begin position="1235"/>
            <end position="1247"/>
        </location>
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            <dbReference type="ARBA" id="ARBA00004651"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026684.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
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    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005028071.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="98" mass="9766" checksum="1BB42ECDA225B1FC" modified="2022-01-19" version="1">TPRAAQRRRPPRAAFGHSENSGAASRPRAHGPAHPAARGSRRPHSPGAPDRAQAAARTPSRATSAGGRAPRPGSRPPAGAAGPGPPRALAAAERGCAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MMM2</accession>
    <name>A0A8C4MMM2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">POU domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">POU2F1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with POU2AF1; the interaction increases POU2F1 transactivation activity. Interacts with NR3C1, AR, PGR and HCFC1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the POU transcription factor family. Class-2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MMM2"/>
    <dbReference type="Ensembl" id="ENSEAST00005030527.2">
        <property type="protein sequence ID" value="ENSEASP00005028107.2"/>
        <property type="gene ID" value="ENSEASG00005019117.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157831"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000979">
        <property type="term" value="F:RNA polymerase II core promoter sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045892">
        <property type="term" value="P:negative regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000005">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.40:FF:000001">
        <property type="entry name" value="POU domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013847">
        <property type="entry name" value="POU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045703">
        <property type="entry name" value="POU2F1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000327">
        <property type="entry name" value="POU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050255">
        <property type="entry name" value="POU_domain_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000972">
        <property type="entry name" value="TF_octamer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636">
        <property type="entry name" value="POU DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11636:SF47">
        <property type="entry name" value="POU DOMAIN, CLASS 2, TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pou"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19536">
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    </dbReference>
    <dbReference type="PRINTS" id="PR00029">
        <property type="entry name" value="OCTAMER"/>
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        <property type="entry name" value="POUDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOMEOBOX_1"/>
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    </dbReference>
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        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00035">
        <property type="entry name" value="POU_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00465">
        <property type="entry name" value="POU_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51179">
        <property type="entry name" value="POU_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0010">Activator</keyword>
    <keyword evidence="4 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 11 12" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 11 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="POU-specific" evidence="16">
        <location>
            <begin position="329"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Homeobox" evidence="15">
        <location>
            <begin position="428"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="11">
        <location>
            <begin position="430"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="32"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="116"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
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            <begin position="307"/>
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            <begin position="563"/>
            <end position="607"/>
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            <begin position="32"/>
            <end position="48"/>
        </location>
    </feature>
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            <begin position="49"/>
            <end position="60"/>
        </location>
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            <begin position="130"/>
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            <begin position="408"/>
            <end position="422"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00008879"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
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            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00046969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU361194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005028107.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="793" mass="81299" checksum="59830A4CEAF9E12A" modified="2023-09-13" version="2">MEGGRGEGSRSEGGFIDRAILVKIFKMADGGAASQDESSAAAAAAADSRMNSPSETSKPSMDSEDGSRGTQTNGLDFQKQPVPVGGAISTAQAQAFLGHLHQVQLAGTSLQAAAQSLNVQSKSNEESGDSQQPSQPSQQPSVQAAIPQTQLMLAGGQITGLTLTPAQQQLLLQQAQAQAQLLAAAVQQHSASQQHSAAGATISASAATPMTQIPLSQPIQIAQDLQQLQQLQQQNLNLQQFVLVHPTTNLQPAQFIISQTPQGQQGLLQAQNLLTQLPQQSQANLLQSQPSITLTSQPATPTRTIAATPIQTLPQSQSTPKRIDTPSLEEPSDLEELEQFAKTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTISRFEALNLSFKNMCKLKPLLEKWLNDAENLSSDSALSSPSALSSPGLGIEGLNRRRKKRTSIETNIRVALEKSFLENQKPTSEEITMIADQLNMEKEVIRVWFCNRRQKEKRINPPSSGGTSSSPIKAIFPSPTSLVATTPSLVTSSAATTLTVNPVLPLTSAAVTNLSVTGTTDTASNSTATVISTAPPASSAVTSPSLSPSPSASASTSEASSASETNTTQTTSTPLSSLGTSQVMVTASGLQTAAAAALQGAAQLPANASLAAMAAAAGLNPGLMAPSQFAAGGALLSLNPGTLGSALSPALMSNSTLATIQALASGGSLPITSLDATGNLVFANAGGAPNIVTAPLFLSPQNLSLLTSSPVSLVSAAAASAGSSGPVASLHAASTSAESIQNSLFTVASASGAASTTTTASKAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MN81</accession>
    <name>A0A8C4MN81_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Calpain-12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Calcium-activated neutral proteinase 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CAPN12</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Calcium-regulated non-lysosomal thiol-protease.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MN81"/>
    <dbReference type="Ensembl" id="ENSEAST00005031041.2">
        <property type="protein sequence ID" value="ENSEASP00005028573.2"/>
        <property type="gene ID" value="ENSEASG00005019426.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161901"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004198">
        <property type="term" value="F:calcium-dependent cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00214">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00044">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.380:FF:000011">
        <property type="entry name" value="Calpain 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000225">
        <property type="entry name" value="Calpain-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000464">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033883">
        <property type="entry name" value="C2_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022684">
        <property type="entry name" value="Calpain_cysteine_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022682">
        <property type="entry name" value="Calpain_domain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022683">
        <property type="entry name" value="Calpain_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036213">
        <property type="entry name" value="Calpain_III_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000169">
        <property type="entry name" value="Pept_cys_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001300">
        <property type="entry name" value="Peptidase_C2_calpain_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183">
        <property type="entry name" value="CALPAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10183:SF280">
        <property type="entry name" value="CALPAIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01067">
        <property type="entry name" value="Calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00648">
        <property type="entry name" value="Peptidase_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00704">
        <property type="entry name" value="CALPAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00720">
        <property type="entry name" value="calpain_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00230">
        <property type="entry name" value="CysPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49758">
        <property type="entry name" value="Calpain large subunit, middle domain (domain III)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50203">
        <property type="entry name" value="CALPAIN_CAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00139">
        <property type="entry name" value="THIOL_PROTEASE_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Calpain catalytic" evidence="12">
        <location>
            <begin position="45"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="13">
        <location>
            <begin position="592"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 11">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 11">
        <location>
            <position position="259"/>
        </location>
    </feature>
    <feature type="active site" evidence="10 11">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00075522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR622684-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005028573.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="691" mass="77930" checksum="A96C7C588364106A" modified="2023-09-13" version="2">MACDSRRVTIQLVDEEAGPGTRGPKPFRGQSYEKIRAACLEEGVLFRDPYFPAGPDTLGYDQLGPDSEKTKGVEWMRPHEFCAEPQFICEDMSRTDVCQGSLGNCWFLAAAASLTLYPRLLCRVVPPGQGFQDGYAGVFHFQLWQFGRWVDVVVDDRLPVREGKLMFVRSAQRNEFWAPLLEKAYAKLHGSYEVMRGGHMNEAFVDFTGGVGEVLYLRQGTPGLFSALRHALAKESLVGATALSDRGEYRTEDGLVKGHAYSVTGTHKVSLGFTKVRLLRLRNPWGRVEWTGAWSDSCPRWDALPAEWREALLVKKEDGEFWMELQDFLRHFDTVQICSLSPEVLGPSPAGGGWHIHTFQGRWVRGFNSGGSQPGAETFWTNPQFRLTLLEPDDEDEDEEGPWGGWGAAGARGPAQGGRTPKCTVLLSLIQRNRRRLRAQGLTYLTVGFHVFQVGPRLLGLWDSPRSRALLPSLLRADRSPFCTRRDVSRRCRLRPGHYLVVPSAARAGDEADFTLRVFSERLNGLMTRSLPDQEEELSAPQLQTLLSIALEPARAHARTPRELGLRTCEQLLQCFGHGRSLGLHRFQQLWRHLLEWQATFDKFDEDASGTMNSYELRLALNAAGFHLNNQLTQALTSRYRDSRLRVDFERFVACVAQLTCIFRHCSQHLDGGEGVVCLTHRQWMEVATFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MNX7</accession>
    <name>A0A8C4MNX7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase</fullName>
            <ecNumber evidence="17">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">PLCB3</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Key transducer of G protein-coupled receptor signaling: activated by G(q)/G(11) G alpha proteins downstream of G protein-coupled receptors activation. In neutrophils, participates in a phospholipase C-activating N-formyl peptide-activated GPCR (G protein-coupled receptor) signaling pathway by promoting RASGRP4 activation by DAG, to promote neutrophil functional responses.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + H2O = 1D-myo-inositol 1-phosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:43484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:57880"/>
            <dbReference type="ChEBI" id="CHEBI:58433"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:43485"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:33180"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
        <text evidence="19">Binds 1 Ca(2+) ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="17"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4MNX7"/>
    <dbReference type="Ensembl" id="ENSEAST00005031915.2">
        <property type="protein sequence ID" value="ENSEASP00005029366.2"/>
        <property type="gene ID" value="ENSEASG00005019964.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160539"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005516">
        <property type="term" value="F:calmodulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
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                <person name="C."/>
                <person name="Wang"/>
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                <person name="Zhuang"/>
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                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
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                <person name="J."/>
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        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="250"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="392"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005032748.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="507" mass="55868" checksum="3C4809F1FB9E1CE1" modified="2023-09-13" version="2">MFLNLSRCKNLLLTGGLDRIIRVWNPYLPRKPTGMLKSHTAPVSYIQVSAEDNKIFSTSTDNAVKVSALWCSDAGLKGELAACLYLPGPRALWVASDTAALLHLRLRSPPEPHLVLSHKEPVGCCWYDPAFWQVVSCSEASVSVNLWDFKGEEGRLLSEFTEAHDNAGITCLTFDSGGRRYVLITGGRDGCLKIWSYNNEHCLHSLKHGNVLAARFTTCTTAPVWLWIQGTVHDFHHFWKPQPHWQDDLSHGHKEDILCVAQCPPFLLATSSYDGEIIIWNVISGHIYCKLNTSTGAQSLSPAGLSNSAVGKAQTPRCPATASLLAGSVTFWRLFGGGGLSHYKAWVSSMVVTAGDALACVADQESFVRVYDIKEYSLWGPELRPPKNVTFWKAHVSMVTSLELIEEEKFLLSSSLDRTVRLWSMDGEYTGTFGQSSPWDVFTPASWSHPRVPYEILTDAQSMPAHPVLEGDVPATHLGKEEQENAVEGKARAKVGSGTSYSSVFSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MW66</accession>
    <name>A0A8C4MW66_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Sp4 transcription factor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">SP4</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Sp1 C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044622055.1">
        <property type="nucleotide sequence ID" value="XM_044766120.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MW66"/>
    <dbReference type="SMR" id="A0A8C4MW66"/>
    <dbReference type="Ensembl" id="ENSEAST00005034669.2">
        <property type="protein sequence ID" value="ENSEASP00005031841.1"/>
        <property type="gene ID" value="ENSEASG00005021731.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106824363"/>
    <dbReference type="CTD" id="6671"/>
    <dbReference type="GeneTree" id="ENSGT00940000157827"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd22536">
        <property type="entry name" value="SP4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000014">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000026">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000061">
        <property type="entry name" value="Transcription factor Sp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039938">
        <property type="entry name" value="Sp4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14947:SF23">
        <property type="entry name" value="SP4 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14947">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="647"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="677"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="707"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="109"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="279"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="347"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="37"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="109"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="121"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="137"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="283"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="347"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="366"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005031841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="784" mass="81918" checksum="AB018F9EE7BBDF38" modified="2022-01-19" version="1">MSDQKKEEEEEAAAAAAMATEGGKTSEPENNNKKPKTSGSQDSQPSPLALLAATCSKIGTPGENQATGQQQIIIDPSQGLVQLQNQPQQLELVTTQLAGNAWQLVASTPPASKENNVSQPASSSSSSSSSNNGSASPTKAKSGNSSTPGQFQVIQVQNPSGSIQYQVIPQLQTVEGQQIQINPTSTSSLQDLQGQIQLISAGNNQAILTAANRTASGNILAQNLANQTVPVQIRPGVSIPLQLQTLPGTQAQVVTTLPINIGGVTLALPVINSVAAGGGTGQVGQPTTTTDSGTSNGSQLVSTPTTTTASASTMPESPSSSTTCTTTASTSLTSSDTLVSSADAGQYASTSASSSERTIEESQTPAATESEAQSSSQLQSNGIQNTQDQSNSLQQVQIVGQPILQQIQIQQPQQQIIQAIPPQSFQLQSGQTIQTIQQQPLQNVQLQAVNPTQVLIRAPTLTPSGQISWQTVQVQNIQSLSNLQVQNAGLSQQLTITPVSSSGGTTLAQIAPVAVAGAPITLNTAQLASVPNLQTVSVANLGAAGVQVQGVPVTITSVAGQQQGQDGVKVQQATIAPVTVAVGGIANATIGAVSPDQLTQVHLQQGQQASDQEVQPGKRLRRVACSCPNCREGEGRGSNEPGKKKQHICHIEGCGKVYGKTSHLRAHLRWHTGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKRFECPECSKRFMRSDHLSKHVKTHQNKKGGGTALAIVTSGELDSSVTEVLGSPRIVTVAAISQDSNPATPNVSTNMEEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MX13</accession>
    <name>A0A8C4MX13_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Interleukin-18 receptor accessory protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Accessory protein-like</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">CD218 antigen-like family member B</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">IL-1R accessory protein-like</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Interleukin-18 receptor accessory protein-like</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">Interleukin-18 receptor beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">IL18RAP</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Within the IL18 receptor complex, does not mediate IL18-binding, but involved in IL18-dependent signal transduction, leading to NF-kappa-B and JNK activation. May play a role in IL18-mediated IFNG synthesis from T-helper 1 (Th1) cells.</text>
    </comment>
    <comment type="subunit">
        <text evidence="17">Forms a ternary complex with IL18 and IL18R1. Within this complex, IL18R1 is involved in ligand-binding and IL18RAP in signaling leading to NF-kappa-B and JNK activation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the interleukin-1 receptor family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014709600.1">
        <property type="nucleotide sequence ID" value="XM_014854114.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070367656.1">
        <property type="nucleotide sequence ID" value="XM_070511555.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MX13"/>
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        <property type="protein sequence ID" value="ENSEASP00005032356.1"/>
        <property type="gene ID" value="ENSEASG00005022061.2"/>
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    <dbReference type="KEGG" id="eai:106839405"/>
    <dbReference type="CTD" id="8807"/>
    <dbReference type="GeneTree" id="ENSGT01090000259985"/>
    <dbReference type="OMA" id="YPHIIQD"/>
    <dbReference type="OrthoDB" id="6019866at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045092">
        <property type="term" value="C:interleukin-18 receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015026">
        <property type="term" value="F:coreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004908">
        <property type="term" value="F:interleukin-1 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042008">
        <property type="term" value="F:interleukin-18 receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008283">
        <property type="term" value="P:cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042119">
        <property type="term" value="P:neutrophil activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045954">
        <property type="term" value="P:positive regulation of natural killer cell mediated cytotoxicity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10140:FF:000002">
        <property type="entry name" value="Interleukin 1 receptor accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001504">
        <property type="entry name" value="Interleukin 18 receptor accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001761">
        <property type="entry name" value="Interleukin 18 receptor accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10140">
        <property type="entry name" value="Toll/interleukin-1 receptor homology (TIR) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015621">
        <property type="entry name" value="IL-1_rcpt_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004074">
        <property type="entry name" value="IL-1_rcpt_I/II-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041416">
        <property type="entry name" value="IL-1RAcP-like_ig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000157">
        <property type="entry name" value="TIR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035897">
        <property type="entry name" value="Toll_tir_struct_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890">
        <property type="entry name" value="INTERLEUKIN-1 RECEPTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11890:SF23">
        <property type="entry name" value="INTERLEUKIN-18 RECEPTOR ACCESSORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13895">
        <property type="entry name" value="Ig_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18452">
        <property type="entry name" value="Ig_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01582">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01536">
        <property type="entry name" value="INTRLKN1R12F"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01537">
        <property type="entry name" value="INTRLKN1R1F"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00255">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52200">
        <property type="entry name" value="Toll/Interleukin receptor TIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50104">
        <property type="entry name" value="TIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="14" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="10 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0520">NAD</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 25" id="KW-0732">Signal</keyword>
    <keyword evidence="4 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 24" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034811640" description="Interleukin-18 receptor accessory protein" evidence="25">
        <location>
            <begin position="20"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="358"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="27">
        <location>
            <begin position="148"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="27">
        <location>
            <begin position="250"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="TIR" evidence="26">
        <location>
            <begin position="405"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00058813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00063675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00075050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00075390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00075897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00079797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00080532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005032356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="585" mass="67226" checksum="71495F1C5E68BE22" modified="2022-01-19" version="1">MLYLSWLFLWLVAGEKIKGFNISDCSTKSILWTYSAGYEEGFFLFCDLPEPQKFDFYHRSQLLPTQGPEHLPCRGVKDLSDVQWYVQPQNGDLLEEITENYPHIIQDKNTLRFLTTRMNNAGTYICRPRIRSPQHEACCVKTVLEVKPETNTSCASSVSHKQYLLLGSTDSIYCPSLSCHSDAQSPKVTWYKNGKLLSEERSNPILVDEIYDYHQGTYVCDYTQSDSTSSWTVRAVVQVRTIVKDTNLKPDILDPIKDTLEVELGKPLTLNCTARFGFEKDFRPVIRWYVKDSDQEWEVPTPEGKSVKSTLKDEVIERVLLLKEVTQSDLRRKFICFAQNSLGNTTQSIQLKEKKGVVFLYILLGTIMTLAGVLTAGAFLYTYWIEIVLLYRTYQSKDETLGDKKEFDAFVSYARWSSFDSEAPSSLSEENLALNLFPEVLENKYGYTLCLFERDVAPGGVYAEDIVSVIKKSRRGIFILSPNYVNGPSVFELQAAVNLALDDQTLKLILIKFRSFREPESLPHLVKKALRVLPTVTWRGLKSVSPNSRFWTQMHYHMPVKNSKGFTWHRLRIIPRIFSLEKTPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MZL9</accession>
    <name>A0A8C4MZL9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tudor domain-containing protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">TDRD7</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of specific cytoplasmic RNA granules involved in post-transcriptional regulation of specific genes: probably acts by binding to specific mRNAs and regulating their translation. Required for lens transparency during lens development, by regulating translation of genes such as CRYBB3 and HSPB1 in the developing lens. Also required during spermatogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. Found in a complex containing CABLES1, CDK16 and CDK17. Interacts with CABLES1, CDK17 and PIWIL1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TDRD7 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_044779171.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MZL9"/>
    <dbReference type="Ensembl" id="ENSEAST00005036274.2">
        <property type="protein sequence ID" value="ENSEASP00005033265.2"/>
        <property type="gene ID" value="ENSEASG00005022765.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106842938"/>
    <dbReference type="CTD" id="23424"/>
    <dbReference type="GeneTree" id="ENSGT00890000139482"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043186">
        <property type="term" value="C:P granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070306">
        <property type="term" value="P:lens fiber cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002089">
        <property type="term" value="P:lens morphogenesis in camera-type eye"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030719">
        <property type="term" value="P:P granule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034587">
        <property type="term" value="P:piRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09986">
        <property type="entry name" value="LOTUS_1_TDRD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09973">
        <property type="entry name" value="LOTUS_2_TDRD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09974">
        <property type="entry name" value="LOTUS_3_TDRD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20427">
        <property type="entry name" value="Tudor_TDRD7_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20428">
        <property type="entry name" value="Tudor_TDRD7_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20429">
        <property type="entry name" value="Tudor_TDRD7_rpt3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="tudor domain-containing protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tudor domain-containing protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.610:FF:000008">
        <property type="entry name" value="Tudor domain-containing protein 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000045">
        <property type="entry name" value="tudor domain-containing protein 7 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="tudor domain-containing protein 7 isoform X2"/>
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        <property type="entry name" value="Tudor domain-containing protein 7 isoform X2"/>
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        <property type="match status" value="3"/>
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        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="LOTUS domain-like"/>
        <property type="match status" value="3"/>
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    <dbReference type="InterPro" id="IPR041966">
        <property type="entry name" value="LOTUS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025605">
        <property type="entry name" value="OST-HTH/LOTUS_dom"/>
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    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
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    <dbReference type="InterPro" id="IPR037978">
        <property type="entry name" value="TDRD7_LOTUS_3"/>
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        <property type="entry name" value="Tudor"/>
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        <property type="entry name" value="Tudor_domain_containing"/>
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    <dbReference type="InterPro" id="IPR047449">
        <property type="entry name" value="Tudor_TDRD7_rpt3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948">
        <property type="entry name" value="TUDOR DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22948:SF14">
        <property type="entry name" value="TUDOR DOMAIN-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12872">
        <property type="entry name" value="OST-HTH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00567">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00333">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63748">
        <property type="entry name" value="Tudor/PWWP/MBT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51644">
        <property type="entry name" value="HTH_OST"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50304">
        <property type="entry name" value="TUDOR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0221">Differentiation</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0744">Spermatogenesis</keyword>
    <feature type="domain" description="HTH OST-type" evidence="14">
        <location>
            <begin position="3"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="HTH OST-type" evidence="14">
        <location>
            <begin position="233"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="HTH OST-type" evidence="14">
        <location>
            <begin position="337"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="13">
        <location>
            <begin position="513"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="domain" description="Tudor" evidence="13">
        <location>
            <begin position="703"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="113"/>
            <end position="157"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="854"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="854"/>
            <end position="865"/>
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        <location>
            <begin position="866"/>
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    <evidence type="ECO:0000313" key="16">
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N130</accession>
    <name>A0A8C4N130_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Glucocorticoid modulatory element binding protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">GMEB2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_070486304.1"/>
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        <property type="protein sequence ID" value="ENSEASP00005033878.1"/>
        <property type="gene ID" value="ENSEASG00005023167.2"/>
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    <dbReference type="KEGG" id="eai:106836309"/>
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    <dbReference type="GeneTree" id="ENSGT00410000025596"/>
    <dbReference type="OMA" id="CEDNSEW"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.390.10:FF:000003">
        <property type="entry name" value="glucocorticoid modulatory element-binding protein 1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.390.10">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059099">
        <property type="entry name" value="GMEB1/2/Spe-44_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010919">
        <property type="entry name" value="SAND-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000770">
        <property type="entry name" value="SAND_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10417">
        <property type="entry name" value="GLUCOCORTICOID MODULATORY ELEMENT-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10417:SF2">
        <property type="entry name" value="GLUCOCORTICOID MODULATORY ELEMENT-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01342">
        <property type="entry name" value="SAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25892">
        <property type="entry name" value="Spe-44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00258">
        <property type="entry name" value="SAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63763">
        <property type="entry name" value="SAND domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50864">
        <property type="entry name" value="SAND"/>
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    <keyword evidence="8" id="KW-0804">Transcription</keyword>
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            <begin position="56"/>
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            <begin position="426"/>
            <end position="460"/>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50864"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005033878.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="653" mass="69342" checksum="25C8E2BB67008666" modified="2022-01-19" version="1">MCEGCAPIALPSARPRLGFGQVPRGAAVSEGLRTAFADPDFTPFGGVPRSERLDRVAVLFSVFGGTSILFSVVAAPVYIPVSSAQHNTARQCASVLLRTWDPGRVTRLVLGILFSCAMATPDVSVHMEEVVVVTTPDTAVDGSGVEEVKTVLVTTNLAPHGGDLTEDNMETENAAAAAAAAFTASSQLKEAVLVKMAEEEENLEAEIVYPITCGDSRANLIWRKFVCPGINVKCVQYDEHVISPKEFVHLAGKSTLKDWKRAIRMNGIMLRKIMDSGELDFYQHDKVCSNTCRSTKIDLSGARVSLSSPTSAEYIPLTPATADVNGSPATITIETCEDPSDWTTAIGDDTFAFWRGLKDAGLLDEVIQEFHQELVETMKGLQQRVQDPPLQLRDAVLLNNIVQNFGMLDLVKKVLASHKCQMDRSREQYARDLAALEQQCDEHRRRAKELKHKSQHLSNVLMTLTPVSLPPPVKRPRLARATSGPAAIASQVLAQSAQIALAPGMPVSQLTSVPLGKVVSTLPSPVLGKGSPQAAPASSPASPLLGGYTVLASSGTTFPSAVEIHPDASSLTVLSTAAMQDGSTVVKVVSPLQLLTLPGLGPTLQNVAQVSPGGSTIVTVPTGAMESAVAAPGPEEHTATIEVAALAEGHEHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N2B6</accession>
    <name>A0A8C4N2B6_EQUAS</name>
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        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106846904</name>
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    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Metazoa</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cell membrane</location>
            <topology evidence="2 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4N2B6"/>
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        <property type="protein sequence ID" value="ENSEASP00005034410.2"/>
        <property type="gene ID" value="ENSEASG00005023485.2"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15421">
        <property type="entry name" value="7tmA_OR2T-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000008">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="140"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
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        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005034410.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="314" mass="34630" checksum="2F0F02F5E8FDEE1F" modified="2023-09-13" version="2">MGESNLSMTSDFTLMGLFSHSGPPLVLLSLVVTIFTVGLLGNTVLLFLICTDSRLHTPMYFLLSQLSLLDVGFPLVTIPKMAIDFLQGESSISFQGCGAQMFFLMLMGVSEGVVLSFMSYDRYVAVCHPLHYPLLMRRQVCLLMMGTSWLSGVLVACIQTSIILHFPYCASRAVDHFFCELPALLKLSCADTSAYDLALSISGVLILLLPLSLIATSYSQVLGAIFRMHSAEARHKAFTTCCSHIAVVGLFYGAAVFMYMVPGTYHSPQQDNVVSLFYSLVTPTLNPLIYSLRNREVRMALVKVLDRAGFRSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N695</accession>
    <name>A0A8C4N695_EQUAS</name>
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        <submittedName>
            <fullName evidence="5">Serpin family D member 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">SERPIND1</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the serpin family.</text>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3 12">Endoplasmic reticulum lumen</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the ClpA/ClpB family. Torsin subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PKJ1"/>
    <dbReference type="Ensembl" id="ENSEAST00005012971.2">
        <property type="protein sequence ID" value="ENSEASP00005011936.1"/>
        <property type="gene ID" value="ENSEASG00005008361.2"/>
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    <dbReference type="GeneTree" id="ENSGT00950000182888"/>
    <dbReference type="OMA" id="ECCDDRS"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
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        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019894">
        <property type="term" value="F:kinesin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071763">
        <property type="term" value="P:nuclear membrane organization"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034504">
        <property type="term" value="P:protein localization to nucleus"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Torsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049337">
        <property type="entry name" value="TOR1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010448">
        <property type="entry name" value="Torsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017378">
        <property type="entry name" value="Torsin_1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10760">
        <property type="entry name" value="TORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10760:SF14">
        <property type="entry name" value="TORSIN-1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21376">
        <property type="entry name" value="TOR1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06309">
        <property type="entry name" value="Torsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038079">
        <property type="entry name" value="Torsin_2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 12" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
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    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Torsin-1A C-terminal" evidence="14">
        <location>
            <begin position="290"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="120"/>
            <end position="127"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR038079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR038079-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF21376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="354" mass="39619" checksum="8B6C67C87ADE6888" modified="2022-01-19" version="1">MPAPSVAAVAGWERGMRRARRLVAAAVLRLLLAAHVAAAFDPVSVGLVIGAASALTGYLSYCRFAECCREEQPLNASALKLDLEEKLFGQHLATEVILKALTGFKNNKNPKKPLTLSLHGWAGTGKNFVSQIVAENLHSKGLKSKFVHLFVSTLHFPHEQQIKLYQDQLQKWIRGNVSACASSLFIFDEMDKLHPGVIDAIKPFLDYYEQVDGVSYRKAIFIFLSNAGGDLITKTALDFWRAGRKREDIELKDLEHVLSVGVFNNKHSGLWHSSLIERNLIDYFIPFLPLEYRHVKMCVRAEMRARGSAIDEDIVTMVAEEMTFFPKDEKIYSDKGCKTVQSRLDFHEAPNGAG</sequence>
</entry>
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    <accession>A0A8C4PSR7</accession>
    <name>A0A8C4PSR7_EQUAS</name>
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        <recommendedName>
            <fullName evidence="5">Guanine nucleotide-binding protein-like 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">GNL1</name>
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    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Possible regulatory or functional link with the histocompatibility cluster.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014687790.1">
        <property type="nucleotide sequence ID" value="XM_014832304.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PSR7"/>
    <dbReference type="Ensembl" id="ENSEAST00005029652.2">
        <property type="protein sequence ID" value="ENSEASP00005027306.2"/>
        <property type="gene ID" value="ENSEASG00005018610.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106825505"/>
    <dbReference type="KEGG" id="eai:106825505"/>
    <dbReference type="CTD" id="2794"/>
    <dbReference type="GeneTree" id="ENSGT00940000158047"/>
    <dbReference type="OrthoDB" id="391988at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01857">
        <property type="entry name" value="HSR1_MMR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043358">
        <property type="entry name" value="GNL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709:SF3">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45709">
        <property type="entry name" value="LARGE SUBUNIT GTPASE 1 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="7">
        <location>
            <begin position="178"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="547"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="550"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037770"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00039902"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS51721"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I7W9</accession>
    <name>A0A9L0I7W9_EQUAS</name>
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        <submittedName>
            <fullName evidence="8">ADAMTS like 5</fullName>
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    <gene>
        <name evidence="8" type="primary">ADAMTSL5</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0I7W9"/>
    <dbReference type="Ensembl" id="ENSEAST00005077676.1">
        <property type="protein sequence ID" value="ENSEASP00005036557.1"/>
        <property type="gene ID" value="ENSEASG00005021735.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160456"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.830:FF:000001">
        <property type="entry name" value="A disintegrin and metalloproteinase with thrombospondin motifs 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.830">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013273">
        <property type="entry name" value="ADAMTS/ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050439">
        <property type="entry name" value="ADAMTS_ADAMTS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045371">
        <property type="entry name" value="ADAMTS_CR_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010294">
        <property type="entry name" value="ADAMTS_spacer1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723">
        <property type="entry name" value="ADAMTS A DISINTEGRIN AND METALLOPROTEASE WITH THROMBOSPONDIN MOTIFS PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13723:SF173">
        <property type="entry name" value="ADAMTS-LIKE PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19236">
        <property type="entry name" value="ADAMTS_CR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05986">
        <property type="entry name" value="ADAMTS_spacer1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01857">
        <property type="entry name" value="ADAMTSFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="ADAMTS/ADAMTS-like cysteine-rich" evidence="7">
        <location>
            <begin position="245"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="ADAMTS/ADAMTS-like Spacer 1" evidence="6">
        <location>
            <begin position="351"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="469"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="655"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="481"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
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            <begin position="199"/>
            <end position="233"/>
        </location>
    </feature>
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        <location>
            <begin position="203"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="4">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR613273-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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            <dbReference type="Pfam" id="PF05986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005036557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IBK6</accession>
    <name>A0A9L0IBK6_EQUAS</name>
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        <recommendedName>
            <fullName evidence="8">Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1</fullName>
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            <fullName evidence="10">Penta-phosphate guanosine-3'-pyrophosphohydrolase</fullName>
        </alternativeName>
    </protein>
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        <name evidence="15" type="primary">HDDC3</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">ppGpp hydrolyzing enzyme involved in starvation response.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>guanosine 3',5'-bis(diphosphate) + H2O = GDP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14253"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:77828"/>
            <dbReference type="EC" id="3.1.7.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the MESH1 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.7.2" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0IBK6"/>
    <dbReference type="Ensembl" id="ENSEAST00005078388.1">
        <property type="protein sequence ID" value="ENSEASP00005038269.1"/>
        <property type="gene ID" value="ENSEASG00005006697.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011608"/>
    <dbReference type="OMA" id="PPWRERK"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008893">
        <property type="term" value="F:guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000012">
        <property type="entry name" value="HD domain containing 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052194">
        <property type="entry name" value="MESH1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46246">
        <property type="entry name" value="GUANOSINE-3',5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE MESH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46246:SF1">
        <property type="entry name" value="GUANOSINE-3',5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE MESH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13328">
        <property type="entry name" value="HD_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="14">
        <location>
            <begin position="91"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00040793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00041770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00079108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="238" mass="26443" checksum="20F4F6785BD1498A" modified="2023-09-13" version="1">MLRTAAQHPALSAGRFSPGGTRPGHTPPSPGLRGARGRCGRSVRAPRAPGTAVVRRTRAMGSEAARLLEAADFAARKHRWQRRKDPEGTPYINHPIGVARILTHEAGITDIVVLQAALLHDTVEDTDTTLDEVELHFGAQVRRLVEEVTDDKTLPKLERKRLQVEQAPHSSPGAKLVKLADKLYNLRDLNRCTPEGWSEQRVQEYFEWAAQVVKGLQGTNRQLEEALKQLLKERGLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0ID02</accession>
    <name>A0A9L0ID02_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleolar protein 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">NOL12</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Multifunctional RNA binding protein that plays a role in RNA metabolism and DNA maintenance. Participates in the resolution of DNA stress and the maintenance of genome integrity by localizing to sites of DNA insults. Also plays a role in proper nucleolar organization by limiting nucleolar size and regulating nucleolar number. Mechanistically, regulates the nucleolar levels of fibrillarin and nucleolin, two key players in pre-rRNA processing and ribosome assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with KIAA1191.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRP17 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0ID02"/>
    <dbReference type="Ensembl" id="ENSEAST00005067829.1">
        <property type="protein sequence ID" value="ENSEASP00005038762.1"/>
        <property type="gene ID" value="ENSEASG00005036935.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015973"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019186">
        <property type="entry name" value="Nucleolar_protein_12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14577">
        <property type="entry name" value="NUCLEOLAR PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14577:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09805">
        <property type="entry name" value="Nop25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="195"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="107"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="241"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="270"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="285" mass="31041" checksum="1547C2BEC69652DB" modified="2023-09-13" version="1">MLLHGGGGGGGGGGGGGGGGGGGGAAPLKGGAWRAELRCTAAARRPRKGENAAPGSVSGGWASRRPGLSAMGRSKKKKQRDGDDRRPRLVLSFDEEKRREYLTGFHKRKVERKKAAIEEIKQRLKEEQRKLREERHQEYLKMLAEREEALEEADELDRLVTAKTESVQYDHPNHTVTVTTISDLDLSGARLLGLPSAEQGAGHGSEEEAASVEKPTRALPRKSRDPLLSQRISSLTASLHAHSRKKVKRKHPRRAQDSTKKPPSATRTSKSQRRRLTGKARHGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IGS6</accession>
    <name>A0A9L0IGS6_EQUAS</name>
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        <recommendedName>
            <fullName evidence="8">40S ribosomal protein S8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RPS8</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the small ribosomal subunit. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Part of the small subunit (SSU) processome, composed of more than 70 proteins and the RNA chaperone small nucleolar RNA (snoRNA) U3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the eukaryotic ribosomal protein eS8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014683572.1">
        <property type="nucleotide sequence ID" value="XM_014828086.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IGS6"/>
    <dbReference type="Ensembl" id="ENSEAST00005064914.1">
        <property type="protein sequence ID" value="ENSEASP00005040135.1"/>
        <property type="gene ID" value="ENSEASG00005027242.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106822751"/>
    <dbReference type="KEGG" id="eai:106822751"/>
    <dbReference type="CTD" id="6202"/>
    <dbReference type="GeneTree" id="ENSGT00390000012433"/>
    <dbReference type="OrthoDB" id="1703270at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022626">
        <property type="term" value="C:cytosolic ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11380">
        <property type="entry name" value="Ribosomal_S8e_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.168.20:FF:000001">
        <property type="entry name" value="40S ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.70:FF:000004">
        <property type="entry name" value="40S ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.70:FF:000005">
        <property type="entry name" value="40S ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.168.20">
        <property type="entry name" value="Ribosomal protein S8e, subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001047">
        <property type="entry name" value="Ribosomal_eS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018283">
        <property type="entry name" value="Ribosomal_eS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042563">
        <property type="entry name" value="Ribosomal_protein_eS8_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00307">
        <property type="entry name" value="eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10394">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01193">
        <property type="entry name" value="RIBOSOMAL_S8E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="8"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005040135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="208" mass="24191" checksum="594E5571B59545B1" modified="2023-09-13" version="1">MGISRDNWHKRRKTGGKRKPYHKKRKYELGRPAANTKIGPRRIHTVRVRGGNKKYRALRLDVGNFSWGSECCTRKTRIIDVVYNASNNELVRTKTLVKNCIVLVDSTPYRQWYESHYALPLGRKKGAKLTPEEEEILNKKRSKKIQKKYDERKKNAKISSLLEEQFQQGKLLACIASRPGQCGRADGYVLEGKELEFYLRKIKARKGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IJJ7</accession>
    <name>A0A9L0IJJ7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Voltage-dependent calcium channel gamma-3 subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Neuronal voltage-gated calcium channel gamma-3 subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Transmembrane AMPAR regulatory protein gamma-3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CACNG3</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="17">The L-type calcium channel is composed of five subunits: alpha-1, alpha-2/delta, beta and gamma. Acts as an auxiliary subunit for AMPA-selective glutamate receptors (AMPARs). Found in a complex with GRIA1, GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG2, CACNG4, CACNG5, CACNG7 and CACNG8. Interacts with AP4M1 and GRIA1; associates GRIA1 with the adaptor protein complex 4 (AP-4) to target GRIA1 to the somatodendritic compartment of neurons.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Membrane</location>
            <topology evidence="1 18">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014685236.1">
        <property type="nucleotide sequence ID" value="XM_014829750.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IJJ7"/>
    <dbReference type="Ensembl" id="ENSEAST00005047697.1">
        <property type="protein sequence ID" value="ENSEASP00005037950.1"/>
        <property type="gene ID" value="ENSEASG00005026147.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106823836"/>
    <dbReference type="KEGG" id="eai:106823836"/>
    <dbReference type="CTD" id="10368"/>
    <dbReference type="GeneTree" id="ENSGT01050000244893"/>
    <dbReference type="OMA" id="YEQDTSE"/>
    <dbReference type="OrthoDB" id="9990458at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032281">
        <property type="term" value="C:AMPA glutamate receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098839">
        <property type="term" value="C:postsynaptic density membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098685">
        <property type="term" value="C:Schaffer collateral - CA1 synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036477">
        <property type="term" value="C:somatodendritic compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016247">
        <property type="term" value="F:channel regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005245">
        <property type="term" value="F:voltage-gated calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099590">
        <property type="term" value="P:neurotransmitter receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099645">
        <property type="term" value="P:neurotransmitter receptor localization to postsynaptic specialization membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098943">
        <property type="term" value="P:neurotransmitter receptor transport, postsynaptic endosome to lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051968">
        <property type="term" value="P:positive regulation of synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098970">
        <property type="term" value="P:postsynaptic neurotransmitter receptor diffusion trapping"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019226">
        <property type="term" value="P:transmission of nerve impulse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.150:FF:000002">
        <property type="entry name" value="Voltage-dependent calcium channel gamma-2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051072">
        <property type="entry name" value="CACNG_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004031">
        <property type="entry name" value="PMP22/EMP/MP20/Claudin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008368">
        <property type="entry name" value="VDCC_gsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12107">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12107:SF5">
        <property type="entry name" value="VOLTAGE-DEPENDENT CALCIUM CHANNEL GAMMA-3 SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00822">
        <property type="entry name" value="PMP22_Claudin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01792">
        <property type="entry name" value="VDCCGAMMA"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18" id="KW-0106">Calcium</keyword>
    <keyword evidence="6 18" id="KW-0107">Calcium channel</keyword>
    <keyword evidence="5 18" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="13 18" id="KW-0407">Ion channel</keyword>
    <keyword evidence="11 18" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 18" id="KW-0813">Transport</keyword>
    <keyword evidence="9 18" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="177"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00046938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU363085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005037950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="315" mass="35515" checksum="6EBCFD22197329EC" modified="2023-09-13" version="1">MRMCDRGIQMLITTVGAFAAFSLMTIAVGTDYWLYSRGVCRTKSTSDNETSRKNEEVMTHSGLWRTCCLEGAFRGVCKKIDHFPEDADYEQDTAEYLLRAVRASSIFPILSVTLLFFGGLCVAASEFHRSRHNVILSAGIFFVSAGLSNIIGIIVYISANAGDPGQRDSKKSYSYGWSFYFGAFSFIIAEIVGVVAVHIYIEKHQQLRAKSHSELLKKSTFARLPPYRYRFRRRSSSRSTEPRSRDLSPISKGFHTIPSTDISMFTLSRDPSKITMGTLLNSDRDHAFLQFHNSTPKEFKDSLHNNPANRRTTPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IKI6</accession>
    <name>A0A9L0IKI6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">RIMS-binding protein 3A-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106848279</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
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        <text evidence="2">Belongs to the RIMBP family.</text>
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        <property type="protein sequence ID" value="ENSEASP00005038295.1"/>
        <property type="gene ID" value="ENSEASG00005035039.1"/>
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        <property type="component" value="Chromosome 8"/>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:benzodiazepine receptor binding"/>
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        <property type="entry name" value="SH3_RIM-BP_1"/>
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        <property type="entry name" value="UDM1_RNF168_RNF169-like"/>
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        <property type="entry name" value="RIMS-binding protein 2 isoform X1"/>
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        <property type="entry name" value="RIMS-binding protein 2 isoform X1"/>
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        <property type="entry name" value="RIMS-binding protein 3A-like"/>
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        <property type="entry name" value="RIMS-binding protein 3A-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="3"/>
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        <property type="entry name" value="FN3_dom"/>
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        <property type="entry name" value="FN3_RIM-BP1/2/3"/>
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    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="RIMB1/RIM3A-C-like_N"/>
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        <property type="entry name" value="RIMBP1/2/3"/>
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        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14234">
        <property type="entry name" value="RIM BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14234:SF21">
        <property type="entry name" value="RIMS-BINDING PROTEIN 3A-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RIMB1_N"/>
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        <property type="entry name" value="SH3"/>
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        <property type="entry name" value="Fibronectin type III"/>
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            <end position="913"/>
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<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
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                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="protein sequence ID" value="ENSEASP00005038560.1"/>
        <property type="gene ID" value="ENSEASG00005033972.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154633"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0035023">
        <property type="term" value="P:regulation of Rho protein signal transduction"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:signal transduction"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="CDD" id="cd04402">
        <property type="entry name" value="RhoGAP_ARHGAP20"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rho GTPase-activating protein 20"/>
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        <property type="entry name" value="ARHGAP20-like_RhoGAP"/>
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        <property type="entry name" value="Rho_GTPase_activation_prot"/>
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        <property type="entry name" value="RhoGAP_dom"/>
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        <property type="entry name" value="1700006A11RIK PROTEIN"/>
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        <property type="entry name" value="T-CELL ACTIVATION RHO GTPASE ACTIVATING PROTEIN-RELATED"/>
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        <location>
            <begin position="1"/>
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        </location>
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        <location>
            <begin position="210"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="233"/>
            <end position="244"/>
        </location>
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        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="244" mass="26476" checksum="25DE26916531F8D9" modified="2023-09-13" version="1">MLCFLKQKGPLTEGIFRKSANMKLCRALKETLNAGDKVNLDCESVLVVACVLKDFLRNIPGSVFTSDLYAKWVSITDGENEEEKIAATQRLLDELPRANAELLRYLFGVLHNIQQHSSVNQMTAYNLATCIAPSILCLPNACSAELESDITRKISLVQFLIENCLQIFGEDIASLLGESSMHCDGSEKAADPSTDFGEDIASLLGESSMHCDGSEKAQLQNDAEAPRGVGPPSSLSAMLSVTED</sequence>
</entry>
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        <recommendedName>
            <fullName evidence="3">Neuroblastoma breakpoint family member 5</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    </dbReference>
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        <property type="entry name" value="NEUROBLASTOMA BREAKPOINT FAMILY MEMBER 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14199">
        <property type="entry name" value="NEUROBLASTOMA BREAKPOINT FAMILY MEMBER 6-LIKE PROTEIN"/>
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    </dbReference>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="10512" checksum="764C2FC40905C27D" modified="2023-09-13" version="1">MAVSLGPVSDPGAEMTLLEINQELQSQLEQSKQDFRDLKQKFLVSESTAYILANQLQKYKSGACKHIIESVLGEKLQFQEGKPTEKSTLAEKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IRN0</accession>
    <name>A0A9L0IRN0_EQUAS</name>
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        <recommendedName>
            <fullName evidence="14">Betaine--homocysteine S-methyltransferase 1</fullName>
            <ecNumber evidence="13">2.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">BHMT</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
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                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
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                <person name="Li"/>
                <person name="R."/>
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                <person name="X."/>
                <person name="Yin"/>
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                <person name="Y."/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-homocysteine + glycine betaine = N,N-dimethylglycine + L-methionine</text>
            <dbReference type="Rhea" id="RHEA:22336"/>
            <dbReference type="ChEBI" id="CHEBI:17750"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="ChEBI" id="CHEBI:58251"/>
            <dbReference type="EC" id="2.1.1.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:22337"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16 17">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="16 17">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 16">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.5" evidence="13"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0IRN0"/>
    <dbReference type="Ensembl" id="ENSEAST00005044567.1">
        <property type="protein sequence ID" value="ENSEASP00005043621.1"/>
        <property type="gene ID" value="ENSEASG00005001952.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003122"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047150">
        <property type="term" value="F:betaine-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006577">
        <property type="term" value="P:amino-acid betaine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071267">
        <property type="term" value="P:L-methionine salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.330:FF:000003">
        <property type="entry name" value="Betaine--homocysteine S-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.330">
        <property type="entry name" value="Homocysteine-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051524">
        <property type="entry name" value="BHMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017226">
        <property type="entry name" value="BHMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003726">
        <property type="entry name" value="HCY_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036589">
        <property type="entry name" value="HCY_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46120">
        <property type="entry name" value="BETAINE--HOMOCYSTEINE S-METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46120:SF2">
        <property type="entry name" value="BETAINE--HOMOCYSTEINE S-METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02574">
        <property type="entry name" value="S-methyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037505">
        <property type="entry name" value="Betaine_HMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82282">
        <property type="entry name" value="Homocysteine S-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50970">
        <property type="entry name" value="HCY"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
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                <person name="Yu"/>
                <person name="J."/>
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                <person name="M."/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="8">Involved in maintaining Golgi structure. Stimulates the formation of Golgi stacks and ribbons. Involved in intra-Golgi retrograde transport.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
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    <dbReference type="AlphaFoldDB" id="A0A9L0IZX8"/>
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        <property type="term" value="F:small GTPase binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="InterPro" id="IPR019177">
        <property type="entry name" value="Golgin_subfamily_A_member_5"/>
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        <property type="entry name" value="GOLGIN SUBFAMILY A MEMBER 5"/>
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    <dbReference type="PANTHER" id="PTHR13815">
        <property type="entry name" value="GOLGIN-84"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Golgin_A5"/>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
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    <name>A0A9L0J2Z9_EQUAS</name>
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            <fullName evidence="3">dolichol kinase</fullName>
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        <name type="scientific">Equus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
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                <person name="Q."/>
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                <person name="Ju"/>
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                <person name="Jin"/>
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                <person name="J."/>
                <person name="Liu"/>
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                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <subcellularLocation>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="Polypren_kinase"/>
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        <property type="entry name" value="DOLICHOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13205">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 15-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
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    <feature type="transmembrane region" description="Helical" evidence="10">
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            <end position="248"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="296"/>
            <end position="317"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="473"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005046753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="538" mass="59112" checksum="127C260A8A6072F6" modified="2023-09-13" version="1">MTRQCAPPASGTGAPLSGSVLAEAAVVFAVVLSIHAAVWDRYSWCAVALAVQAFYVQYKWDRLLQQGSAVFRFRTSANSGLLPASVVMPLLGLVMKERCQSAGNPYFERFGIVVAASGMAVALFSSVLALGITRPVPSNTCVISGLAGGVIIYIMKHSLSVGEVIEVLEVLLIFVYLNMILLYLLPRCFTPGEALLVLGGISFMLNQLIRRSLTVVESQGDPVDFFLLVVVVGMVLMGVFFSTLFVFMDSGTWASSIFFHLMTCVLALGVVLPWLHRLIHRNPLLWLLQFLFQTNTRIYLLAYWTLLATLACLVVLYQNAKRSSSESKKHQAPTIARKYFHFIVVATYIPGIVFDRSLLYVAATVCLAVFLFLEYVRYFRIKPLGHTLRSLLSLFLDERDSGPLILTHIYLLLGMSLPIWLVPRPCTQKGSLGGARALVPYAGVLAVGVGDTVASIFGSTMGEIRWPGTKKTFEGTMTSIFAQIISVALILIFDSGVDLNYSYAWILGSISIVSLLEAYTTQIDNLLLPLYLLILLMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J371</accession>
    <name>A0A9L0J371_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">GLI family zinc finger 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">GLI1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GLI C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J371"/>
    <dbReference type="Ensembl" id="ENSEAST00005048666.1">
        <property type="protein sequence ID" value="ENSEASP00005043725.1"/>
        <property type="gene ID" value="ENSEASG00005007134.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160235"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009653">
        <property type="term" value="P:anatomical structure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007224">
        <property type="term" value="P:smoothened signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000019">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000031">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000036">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000048">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000068">
        <property type="entry name" value="GLI family zinc finger 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043359">
        <property type="entry name" value="GLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056436">
        <property type="entry name" value="Znf-C2H2_ZIC1-5/GLI1-3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR CUBITUS INTERRUPTUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45718:SF2">
        <property type="entry name" value="ZINC FINGER PROTEIN GLI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23561">
        <property type="entry name" value="zf-C2H2_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="194"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="227"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="260"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="290"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="321"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="334"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="465"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="577"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="790"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="868"/>
            <end position="997"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1013"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="372"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="401"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="503"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="597"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="811"/>
            <end position="831"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005043725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1065" mass="113400" checksum="0402C617DFE8878E" modified="2023-09-13" version="1">MFNSMTPPPVSSYGEPCCLRPLPSQGAPSMGTEVKLTKKRALSISPLSDASLDLQTVIRTSPSSLVAFINSRCASPGGSYGHLSISTMSPSLGFPAQMNHQKGTSPSFAVQPCGPHDPTHSGMMPHPQSRGPLPTCQLKSELDMLVSKCPEEPLEGDMSSPNSTGTQDPLLGLLDGREDLEREEKPEPESVYETDCRWDGCSQEFDSQEQLVHHINSEHIHGERKEFVCHWGGCSRELRPFKAQYMLVVHMRRHTGEKPHKCTFEGCRKSYSRLENLKTHLRSHTGEKPYMCEHEGCSKAFSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRVPSLSTVEPKRERDGGPIREESRLAVPEGAMKPQPSPGAQSSCSSDHSPAGSAANTDSGVEMTGNAGGSTEDLSSLDEGPCIAGTGLSTLRRLENLRLDQLHQLRPGGPRGLKLPSLTHTGTPVSRRLGPPASLDRRSSSSSSVSSAYTVSRRSSLASPFPPGSPPENGASSLPGLTPAQHYLLRARYASARGGGTPPTAAPSLDRMGGLPAPPWRSRAEYPGYNPNVGVTRRASDPTRAADRPAPARVQRFKSLGCVHTPPTMTGGGPNFDPQLSTSVYSPQPPSITENIAMDTRGLREEPEIGTSMMGSGLNPYMDFPSTDTLGYGGPEGAAAEPYGARGPGSLPLGPGPPTNYGPNPCPQQVSYPDPTPETWGEFPSHTGLYPGPKPSVGAYSQCPRLEHYGQVQVKPEQGCPVGSDSTGLAPCLNAHPNEGPPRPQPLFSHYPQPPPPQYPQAGPYPQPPPDYLPSESRPGLDFDSPTHSTGQLKAQLVCDYVQSQQELLWDGGGRGDPPVQEPLYQSPKFLGGSQVSPNPAKAPVATYGPGFAPNLPHHKSGSYPTPSPCHENFAVGANKSSHQAAAPPRLLPPLPTCYGPLKAGGSNPSCGHPEVGRLGGGPALYPPPEGQVCNPLDSLDLDNTQLDFVAILDEAQGLSPPPAHDQGDSSEHTPPPSGPPNMAVGNMSVLLGSLPGETQFLNSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J460</accession>
    <name>A0A9L0J460_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Solute carrier family 4 member 1 adaptor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">SLC4A1AP</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
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                <person name="D.D."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="nucleotide sequence ID" value="XM_014850390.3"/>
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    <dbReference type="GeneID" id="106837058"/>
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    <dbReference type="CTD" id="22950"/>
    <dbReference type="GeneTree" id="ENSGT00940000155320"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
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        <property type="term" value="C:nucleoplasm"/>
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        <property type="term" value="C:plasma membrane"/>
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    <dbReference type="CDD" id="cd19856">
        <property type="entry name" value="DSRM_Kanadaptin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22677">
        <property type="entry name" value="FHA_Kanadaptin"/>
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        <property type="entry name" value="Solute carrier family 4 member 1 adaptor protein"/>
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        <property type="entry name" value="Cell_Proc_Reg/RNA_Proc"/>
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        <property type="entry name" value="dsRBD_dom"/>
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        <property type="entry name" value="FHA_dom"/>
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    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
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        <property type="entry name" value="NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1"/>
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        <property type="entry name" value="dsrm"/>
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            <begin position="132"/>
            <end position="192"/>
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    <feature type="region of interest" description="Disordered" evidence="2">
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    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="560"/>
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            <begin position="442"/>
            <end position="469"/>
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            <begin position="1"/>
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            <begin position="69"/>
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            <begin position="84"/>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J547</accession>
    <name>A0A9L0J547_EQUAS</name>
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        <recommendedName>
            <fullName evidence="10">Pepsin A</fullName>
            <ecNumber evidence="3">3.4.23.1</ecNumber>
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        <name evidence="16" type="primary">LOC106847581</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="1">Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the peptidase A1 family.</text>
    </comment>
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        <property type="gene ID" value="ENSEASG00005031016.1"/>
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        <property type="component" value="Chromosome 17"/>
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        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05478">
        <property type="entry name" value="pepsin_A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cathepsin E"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Pepsin A"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012848">
        <property type="entry name" value="Aspartic_peptidase_N"/>
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    <dbReference type="InterPro" id="IPR034162">
        <property type="entry name" value="Pepsin_A"/>
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    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF22">
        <property type="entry name" value="PEPSIN A-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07966">
        <property type="entry name" value="A1_Propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="6" id="KW-0222">Digestion</keyword>
    <keyword evidence="9 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 13" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
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            <begin position="16"/>
            <end position="387"/>
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    <feature type="domain" description="Peptidase A1" evidence="15">
        <location>
            <begin position="75"/>
            <end position="384"/>
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    <feature type="active site" evidence="11">
        <location>
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    <feature type="active site" evidence="11">
        <location>
            <position position="276"/>
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            <begin position="106"/>
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    <feature type="disulfide bond" evidence="12">
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            <dbReference type="ARBA" id="ARBA00007447"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00011924"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005047493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="387" mass="41909" checksum="041A3E23C54BBBB8" modified="2023-09-13" version="1">MKWLLLLSLVALSECLIYKVPLVRKKSLRQNLIEHGLLEDFLKKHTPNPASKFFPKEAATLVDSEPLENYLDEEYFGTISIGTPPQEFTVIFDTGSSNLWVPSTYCSSLACYDHKRFNPEKSSTYQATSESISITYGTGSMTGILGYDTVRVGGIEDTNQIFGLSEKEPGFFLFLAPFDGILGLGYPSISASGATPVFDNIWDQGLVSQDLFSVYLSSDDESGSVVMFGGIDSSYYTGSLHWVPVTTEGYWQIAVDSITINGESIACSGGCQAIVDTGTSLLAGPTSGIDNIQSYIGARKDLLGEGVIGCSAIDSLPDIVFTMNGVEFPLPPSAYILKEDDSCISGFEGVDLDTSSGELWILGDVFIRQYFTVFDRANNQVGLAPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J5G3</accession>
    <name>A0A9L0J5G3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ectonucleotide pyrophosphatase/phosphodiesterase family member 3</fullName>
            <ecNumber evidence="4">3.1.4.1</ecNumber>
            <ecNumber evidence="10">3.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Alkaline phosphodiesterase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Dinucleoside polyphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Nucleotide diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">Nucleotide pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Phosphodiesterase I beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Phosphodiesterase I/nucleotide pyrophosphatase 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">LOC106835758</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP. Limits mast cells and basophils response during inflammation and during the chronic phases of allergic responses by eliminating extracellular ATP, a signaling molecule activating these cells in an autocrine manner. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells. Has a broad specificity and can also hydrolyze UDP-GlcNAc into UMP and GlcNAc-1-phosphate and potentially several other intracellular nucleotide sugars, including UDP-GalNAc, CMP-NeuAc, GDP-Fuc, and UDP-GlcA. Thereby, could modulate glycan biosynthesis and protein glycosylation. Can hydrolyze extracellular dinucleoside polyphosphates, including the vasoactive adenosine polyphosphates as well. In addition, displays an alkaline phosphodiesterase activity in vitro.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14245"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:14246"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>CTP + H2O = CMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27762"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:27763"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>GTP + H2O = GMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29391"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:29392"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.</text>
            <dbReference type="EC" id="3.1.4.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>P(1),P(3)-bis(5'-adenosyl) triphosphate + H2O = AMP + ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13893"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:13894"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>P(1),P(4)-bis(5'-adenosyl) tetraphosphate + H2O = AMP + ATP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:32039"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58141"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:32040"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>P(1),P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GMP + GTP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57553"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>P(1),P(5)-bis(5'-adenosyl) pentaphosphate + H2O = adenosine 5'-tetraphosphate + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:32051"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58450"/>
            <dbReference type="ChEBI" id="CHEBI:62041"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:32052"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>UDP-N-acetyl-alpha-D-glucosamine + H2O = N-acetyl-alpha-D-glucosamine 1-phosphate + UMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:29547"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:29548"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>UTP + H2O = UMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29395"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:29396"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-phosphate + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23996"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="EC" id="3.6.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:23997"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer and homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.1.4.1" evidence="4"/>
    <dbReference type="EC" id="3.6.1.9" evidence="10"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0J5G3"/>
    <dbReference type="Ensembl" id="ENSEAST00005075450.1">
        <property type="protein sequence ID" value="ENSEASP00005047608.1"/>
        <property type="gene ID" value="ENSEASG00005031877.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159640"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004528">
        <property type="term" value="F:phosphodiesterase I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009143">
        <property type="term" value="P:nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00091">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.20:FF:000001">
        <property type="entry name" value="Ectonucleotide pyrophosphatase/phosphodiesterase family member 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.570.10:FF:000005">
        <property type="entry name" value="ectonucleotide pyrophosphatase/phosphodiesterase family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.410.20:FF:000004">
        <property type="entry name" value="ectonucleotide pyrophosphatase/phosphodiesterase family member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.720.10:FF:000145">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.410.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.570.10">
        <property type="entry name" value="Extracellular Endonuclease, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044929">
        <property type="entry name" value="DNA/RNA_non-sp_Endonuclease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001604">
        <property type="entry name" value="Endo_G_ENPP1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020821">
        <property type="entry name" value="ENPP1-3/EXOG-like_nuc-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044925">
        <property type="entry name" value="His-Me_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036024">
        <property type="entry name" value="Somatomedin_B-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001212">
        <property type="entry name" value="Somatomedin_B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF107">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE_PHOSPHODIESTERASE FAMILY MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01033">
        <property type="entry name" value="Somatomedin_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00892">
        <property type="entry name" value="Endonuclease_NS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00477">
        <property type="entry name" value="NUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00201">
        <property type="entry name" value="SO"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54060">
        <property type="entry name" value="His-Me finger endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90188">
        <property type="entry name" value="Somatomedin B domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00524">
        <property type="entry name" value="SMB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50958">
        <property type="entry name" value="SMB_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="30" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="30" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="30" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="30">
        <location>
            <begin position="67"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="SMB" evidence="31">
        <location>
            <begin position="94"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="SMB" evidence="31">
        <location>
            <begin position="138"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="29">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="907" mass="102353" checksum="3AC9870080F7D096" modified="2023-09-13" version="1">MECGEGGHGRRVGSAVSGRDPSRGHASPAPGDPQVAESLLAPMEVGEEPLEKAARAGAAKDPNTYKVLSLVLLALLVIVSLGLGLGLGLRQPEKQGSCRRRCFDSSYRGLEGCRCDVGCKDRGDCCWDFEDTCVESTRIWTCNAFRCGETRLESSLCSCSDDCLQRKDCCTNYKSVCQGESPWVQEECGTAQQSRCPEGFDLPPVILFSMDGFRAEYLQTWKTCGIHSKYLRPVYPTKTFPNHYTIVTGLYPESHGIIDNNMYDVNLNKSFSIFSKGTFDPAWWHGQPVWLTAMNQGLKAGTYFWPGSDVAINGSFPSLYMVYNSVPYEERISTLLTWLDLPKAERPDFYTLYVGQPDSAGHEKGPVSAAVIQALQLVDNALGMLMEGLKQRNLDNCVNIILVTDHGMDQTYCEKVEYMTDYLPQVNFYMYEGPAPRIRAYNVPQDFYSFNSEEIVRNLSCRKPDQHFKPYLSPDLPKRLHFAKNIRIDKVHLLVNPPWLAVRSKDFSYCGGGTHGYDNKFKSMEAIFLAHGPSFKEKTEIEPFENIELYNLICDLLHIQPAPNNGTHGSLNHLLKVPFYEPSHEEEVSKFSVCGFTVPLPTDALDCSCPQLQRDTSLEYANQMLNLTQDEITATEKLNLPFGRPRVMQKNKEHCLLYHREYVSGFGKDIRMPLWSSYTVPNPGDTSPLPPTVPDCLRADVRVAPSESQKCSFYLADKNVTHGFLYPPANNRTSESQYDALITSNLVPMYEEFKKIWDYFHSVLLVKHAVERNGVNVVSGPVFDYNYDGRFDASNEIIEYVVNTSVPIPTHYFVVLTSCKNKSHTPDACPGWLDVLPFVIPHRPTNVESCPEGKQEANWVEERLKEHVARVRDVELLTGLDFYQEKVQPVSEILQLKTYLPTFETII</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JCF6</accession>
    <name>A0A9L0JCF6_EQUAS</name>
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        <submittedName>
            <fullName evidence="4">DnaJ heat shock protein family (Hsp40) member B7</fullName>
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        <name evidence="4" type="primary">DNAJB7</name>
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        <name type="scientific">Equus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Tian"/>
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                <person name="Li"/>
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                <person name="Ju"/>
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                <person name="Li"/>
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                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
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                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="3">
        <location>
            <begin position="3"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="290"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="312" mass="36111" checksum="5372496B79E02BAC" modified="2023-09-13" version="1">MVDYYEVLGVQRYASPEDIKKAYHKVALKWHPDKNPENKEEAERKFKEVAEAYEVLSNDKKRDTYDKYGKEGLNGRVRSHFDDSFEYGFTFRKPDDVFREIFGERDPFSFHFFEDSLEDLLNGPRSCYGSRNRGGRCVFSPSSEHSIFERFSSCDTGYTPYGSLGHEGLNSFSSLAFDDSGMGNYISVTTSGKIVNGRNTNTKRIIENDQEREEIEENGELKSSLMKSVTGEEGFVEECSWRRQSFNKYSPKSHSPEHVCEYTFVDNDEQAIPWVTGNWDPLIFSAGFKEGGKRKKKKHKEVQKKKSTKRNR</sequence>
</entry>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Y."/>
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                <person name="J."/>
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                <person name="J."/>
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                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the FXYD family.</text>
    </comment>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017080">
        <property type="term" value="F:sodium channel regulator activity"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:monoatomic ion transport"/>
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        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="FXYD_motif"/>
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    <dbReference type="InterPro" id="IPR000272">
        <property type="entry name" value="Ion-transport_regulator_FXYD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14132:SF15">
        <property type="entry name" value="FXYD DOMAIN-CONTAINING ION TRANSPORT REGULATOR 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14132">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF02038">
        <property type="entry name" value="ATP1G1_PLM_MAT8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01310">
        <property type="entry name" value="FXYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040531401" description="FXYD domain-containing ion transport regulator" evidence="9">
        <location>
            <begin position="19"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="66"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
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        </source>
    </evidence>
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</entry>
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            <fullName evidence="3">BLOC-1 related complex subunit 6</fullName>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSEASG00005033955.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00490000043453"/>
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        <property type="component" value="Chromosome 13"/>
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        <property type="term" value="C:BORC complex"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032418">
        <property type="term" value="P:lysosome localization"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019314">
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    </dbReference>
    <dbReference type="InterPro" id="IPR046465">
        <property type="entry name" value="BORCS6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13440">
        <property type="entry name" value="BLOC-1 RELATED COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13440:SF8">
        <property type="entry name" value="BLOC-1-RELATED COMPLEX SUBUNIT 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10157">
        <property type="entry name" value="BORCS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BLOC-1-related complex subunit 6 C-terminal helix" evidence="2">
        <location>
            <begin position="251"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="354" mass="36926" checksum="87E9C754C15DFFEB" modified="2023-09-13" version="1">MESPRGRPGPEADLLAAGEQQAAILGGGPSRTPSEPPSGLRLSEEEEAENVGGTSPHPRASPKTSRCSVVHPPEREAREDGPGRGGTPSGAGSCRGVPGPEHDPHGSSRRKDPEPPEDKPASERACRRGSPGGGGMDAEQEEEDDEEVAAAGRVGRSFSSRLQDSRSLDGLSGACGGVGSAAGAESGAGGGRRATISSPLELEGTVSRHGDLTHFVANNLQLKIRLSGAPQPPPPAPARPCSMPAPTPAIPPIDPDVLRDLERLSRELGGRVDRLLRGLGGAVQELTALSVGCIQTYRDAVDSLGEAVDMSIKGMYTLLARCEELERALQPVQGLARQVRDIRRTLEVFEALCK</sequence>
</entry>
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    <accession>A0A9L0JE24</accession>
    <name>A0A9L0JE24_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Prefoldin subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">VBP1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eutheria</taxon>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Sun"/>
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                <person name="J."/>
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                <person name="X."/>
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                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
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                <person name="Wang"/>
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                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="3 6">Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterohexamer of two PFD-alpha type and four PFD-beta type subunits. Binds to the C-terminal part of VHL.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014836839.3"/>
    </dbReference>
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    <dbReference type="SMR" id="A0A9L0JE24"/>
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        <property type="protein sequence ID" value="ENSEASP00005047480.1"/>
        <property type="gene ID" value="ENSEASG00005035328.1"/>
    </dbReference>
    <dbReference type="GeneID" id="139039673"/>
    <dbReference type="KEGG" id="eai:106828394"/>
    <dbReference type="CTD" id="7411"/>
    <dbReference type="GeneTree" id="ENSGT00390000018904"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001540">
        <property type="term" value="F:amyloid-beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015631">
        <property type="term" value="F:tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007017">
        <property type="term" value="P:microtubule-based process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905907">
        <property type="term" value="P:negative regulation of amyloid fibril formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007021">
        <property type="term" value="P:tubulin complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23156">
        <property type="entry name" value="Prefoldin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.370:FF:000001">
        <property type="entry name" value="Prefoldin subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016655">
        <property type="entry name" value="PFD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
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    <sequence length="197" mass="22503" checksum="9D20E7CCDFE7A196" modified="2023-09-13" version="1">MAAAKDSCGLGEVAAGNGRRLHLGIPEAVFVEDVDSFMKQPGNETADTVLKKLDEQYQKYKFMELNLAQKKRRLKGQIPEIKQTLEILKYMQKKKESTNSLETRFLLADNLYCKASVPPTDKVCLWLGANVMLEYDIDEAQALLEKNLSTATKNLDSLEEDLDFLRDQFTTTEVNMARVYNWDVKRRNKDDSTKNKA</sequence>
</entry>
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    <accession>A0A9L0JF98</accession>
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        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Yang"/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
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                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="A."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
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        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="7tm_4"/>
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    </dbReference>
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    </dbReference>
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            <end position="49"/>
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    </evidence>
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        </source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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</entry>
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                <person name="Liu"/>
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                <person name="Wang"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Gao"/>
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                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
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                <person name="Hou"/>
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                <person name="G."/>
                <person name="Liu"/>
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                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
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                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="55" mass="6252" checksum="5E9ABA2798A65E8E" modified="2023-09-13" version="1">MATPTVPCHTTSQKMRWLLTWNLLRTCMNCTRRSLQMSSSWAGTLQAMTSQSTLC</sequence>
</entry>
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    <accession>A0A9L0JPU6</accession>
    <name>A0A9L0JPU6_EQUAS</name>
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        <recommendedName>
            <fullName evidence="13">Zinc finger and BTB domain-containing protein 25</fullName>
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    <gene>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">May be involved in transcriptional regulation.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
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    <dbReference type="GeneTree" id="ENSGT00940000159887"/>
    <dbReference type="OMA" id="YCDNWSD"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000075">
        <property type="entry name" value="zinc finger and BTB domain-containing protein 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BTB/POZ_dom"/>
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    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF51">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING 25"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
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    </dbReference>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
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    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
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    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
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<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
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                <person name="Liu"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        </location>
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        <source>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <sequence length="158" mass="18476" checksum="A09AE41D8FBCA96E" modified="2023-09-13" version="1">MAKPDHATSEVYEIMVKCWNSEPEKRPSFYHLSEIVENLLPGQYKKSYEKIHLDFLKSDHPAVARMRVDSDNAYIGVTYKNEEDKLKDWEGGLDEQRLRVRTVATSSLCRTSTLFRRRKTWARGTDTARRPLKRVPSRQVPAVLPSLRERMRPLRTST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JUH1</accession>
    <name>A0A9L0JUH1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">2-oxoglutarate and iron-dependent oxygenase domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">OGFOD3</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>L-ascorbate</name>
            <dbReference type="ChEBI" id="CHEBI:38290"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the OGFOD3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JUH1"/>
    <dbReference type="Ensembl" id="ENSEAST00005050652.1">
        <property type="protein sequence ID" value="ENSEASP00005056088.1"/>
        <property type="gene ID" value="ENSEASG00005005909.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005895"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051213">
        <property type="term" value="F:dioxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031418">
        <property type="term" value="F:L-ascorbic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.620:FF:000019">
        <property type="entry name" value="2-oxoglutarate and iron-dependent oxygenase domain-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039210">
        <property type="entry name" value="OGFOD3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006620">
        <property type="entry name" value="Pro_4_hyd_alph"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14650:SF1">
        <property type="entry name" value="2-OXOGLUTARATE AND IRON-DEPENDENT OXYGENASE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14650">
        <property type="entry name" value="PROLYL HYDROXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00702">
        <property type="entry name" value="P4Hc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0223">Dioxygenase</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0847">Vitamin C</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="17">
        <location>
            <begin position="203"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005056088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="315" mass="34863" checksum="AB4192408D158CD7" modified="2023-09-13" version="1">MAPQRRGAPKAPEGSGAAERRRPNGMKNVRAPRETQRKWLRAATLGACAALTALLLWGSLGGDDGVTEVLAHRSEVLLGRFIEVPCSEDYDSHRRFEGCSPRKCGRGVTDAVITRDEARRIRSIAEKGLSLGGSDGGASILDLHSGALSVGRHFVNLYRYFGDKVQNIFSEEDFQLYRDVRQKVQLAIAQAFGISASSLYLTKPTFFSRINSTEARTAHDEYWHAHVDKVTYGSFDYTSLLYLSDYLDDFGGGRFVFMEEGANKTVEPRAGRVSFFTSGSENLHRVEKVLWGTRYAVTIAFTCDPDHGIADPVLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JYC6</accession>
    <name>A0A9L0JYC6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Zinc finger protein 709-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106834348</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JYC6"/>
    <dbReference type="Ensembl" id="ENSEAST00005074600.1">
        <property type="protein sequence ID" value="ENSEASP00005054110.1"/>
        <property type="gene ID" value="ENSEASG00005026098.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182755"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002568">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000193">
        <property type="entry name" value="Zinc finger protein 300"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000184">
        <property type="entry name" value="Zinc finger protein 333"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000156">
        <property type="entry name" value="Zinc finger protein 568"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050758">
        <property type="entry name" value="Znf_C2H2-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234:SF9">
        <property type="entry name" value="ZINC FINGER PROTEIN 850-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a phosphate monoester + H2O = an alcohol + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15017"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:67140"/>
            <dbReference type="EC" id="3.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.1" evidence="3 12"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0JZ94"/>
    <dbReference type="Ensembl" id="ENSEAST00005048386.1">
        <property type="protein sequence ID" value="ENSEASP00005054425.1"/>
        <property type="gene ID" value="ENSEASG00005026369.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183063"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018299">
        <property type="entry name" value="Alkaline_phosphatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596:SF30">
        <property type="entry name" value="INTESTINAL-TYPE ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00123">
        <property type="entry name" value="ALKALINE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 10 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0732">Signal</keyword>
    <keyword evidence="7 10 12" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040232100" description="Alkaline phosphatase" evidence="14">
        <location>
            <begin position="21"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="9">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="326"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601952-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005054425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="466" mass="50625" checksum="F90C92205F9FA24D" modified="2023-09-13" version="1">MRGAWVLLLLLGLRLRLSLGVIPGCSPARLTQPPLSPWPVEEEDPAFWNRQAAQALDTAKKLQPIQTAAKNLIIFLGDGEYGWGCPTVTATRILKAQMDGKLGPETPLAMDHFPYLALSKTYNVDRQVPDSAGTATAYLCAGATRQTSVVQPHLCHPQGSQWGVVTTSRVQHASPAGTYAHTVNRNWYSDADMPAEALKEGCQDIATQLISNMDIDVILGGGRKYMFPKGTPDPEYPNDASQNGTRVDQRNLVQEWLGAQYVWNRTALIQASQDPSVTHLMGLFEPEDLKYEAHRDLTLDPSLTEMTEAALRVLSRNPSGFYLFVEGLAPSKASDGKAYTSILYGNGPGYVLRGGSRPDINESQSSDRQPYPCRRRPTAARTRRLFARGPQAHLVHGVQEQTFVAHVMAFAACLEPYTACDLSPPATSTDSTNPGLTDAALPRPAAGSLSLQLLVGALLLLLSATH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K139</accession>
    <name>A0A9L0K139_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Tropomyosin 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">TPM2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the tropomyosin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014721548.1">
        <property type="nucleotide sequence ID" value="XM_014866062.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K139"/>
    <dbReference type="SMR" id="A0A9L0K139"/>
    <dbReference type="Ensembl" id="ENSEAST00005039926.1">
        <property type="protein sequence ID" value="ENSEASP00005059089.1"/>
        <property type="gene ID" value="ENSEASG00005027967.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106846982"/>
    <dbReference type="CTD" id="7169"/>
    <dbReference type="GeneTree" id="ENSGT01030000234542"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000013">
        <property type="entry name" value="Tropomyosin 1 (alpha)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000005">
        <property type="entry name" value="Tropomyosin alpha-1 chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.340:FF:000001">
        <property type="entry name" value="Tropomyosin alpha-1 chain isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000533">
        <property type="entry name" value="Tropomyosin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19269">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00261">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00194">
        <property type="entry name" value="TROPOMYOSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00326">
        <property type="entry name" value="TROPOMYOSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0514">Muscle protein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="115"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="12"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005059089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="284" mass="33012" checksum="206AB9B510BA37D1" modified="2023-09-13" version="1">MDAIKKKMQMLKLDKENAIDRAEQAEADKKQAEDRCKQLEEEQQALQKKLKGTEDEVEKYSESVKDAQEKLEQAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIENRAMKDEEKMELQEMQLKEAKHIAEDSDRKYEEVARKLVILEGELERSEERAEVAESRARQLEEELRTMDQALKSLMASEEEYSTKEDKYEEEIKLLEEKLKEAETRAEFAERSVAKLEKTIDDLEDEVYAQKMKYKAISEELDNALNDITSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K3Z6</accession>
    <name>A0A9L0K3Z6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">F-BAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <location evidence="1">Clathrin-coated pit</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FCHO family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K3Z6"/>
    <dbReference type="Ensembl" id="ENSEAST00005044741.1">
        <property type="protein sequence ID" value="ENSEASP00005060221.1"/>
        <property type="gene ID" value="ENSEASG00005005938.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160489"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030136">
        <property type="term" value="C:clathrin-coated vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048268">
        <property type="term" value="P:clathrin coat assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000016">
        <property type="entry name" value="FCH domain only protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054713">
        <property type="entry name" value="GMIP/FCHO2-like_FCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF6">
        <property type="entry name" value="F-BAR DOMAIN ONLY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22699">
        <property type="entry name" value="GMIP-like_FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0168">Coated pit</keyword>
    <keyword evidence="4 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="9">
        <location>
            <begin position="2"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="320"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005060221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="368" mass="40658" checksum="1A901A2CA87355F0" modified="2023-09-13" version="1">LQQLTKVEKLWDGDGEKNHGFDVLYQSMKQGPVSTKELADFIRERATIEETYSKAMAKLSKLASNGTPMGTFAPLWEVFRVSSDKLALCHLELTRKLQDLIKDVLRYGEEQLKAHKKCKEEAVGTLDAVQVLAGVSQLLPKSRENYLNRCMDQERLRRESTNQKEMDKAETKTKKAAESLRRSVEKYNSARADFEQKMLDSALRFQALEETHLRHMKALLGSYAHSVENTHVQIGQVHEEFKQNIENVSVEMLLRKFAESKGTGREKPGPLDFEAYSTAALQEGKYVWAGRPGCAAAGGGRPEREGARLSAPSVLSYEAAAGRQGLSPPGTEPARAGATCSCVRSPHPAPAQASPGPPLPRLAQKSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K5W8</accession>
    <name>A0A9L0K5W8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 subunit J</fullName>
            <shortName evidence="6">eIF3j</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Eukaryotic translation initiation factor 3 subunit 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">eIF-3-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">eIF3 p35</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">EIF3J</name>
        <name evidence="6" type="synonym">EIF3S1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 6">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="6">Phosphorylated. Phosphorylation is enhanced upon serum stimulation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eIF-3 subunit J family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K5W8"/>
    <dbReference type="Ensembl" id="ENSEAST00005073290.1">
        <property type="protein sequence ID" value="ENSEASP00005060866.1"/>
        <property type="gene ID" value="ENSEASG00005018015.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018400"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.60:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.60">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03009">
        <property type="entry name" value="eIF3j"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023194">
        <property type="entry name" value="eIF3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013906">
        <property type="entry name" value="eIF3j"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08597">
        <property type="entry name" value="eIF3_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="3 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="initiator methionine" description="Removed" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="168"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Promotes stable association with the 40S ribosome" evidence="6">
        <location>
            <begin position="194"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="40"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="modified residue" description="N-acetylalanine" evidence="6">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="6">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="6">
        <location>
            <position position="20"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphothreonine" evidence="6">
        <location>
            <position position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00065260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005060866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="209" mass="23059" checksum="58E6480073A1CC7B" modified="2023-09-13" version="1">MAAAAAAAGDSDSWDADTFSVEDPVRKVGGGGTAGGDRWEGEDEDEDVKLEEPEEPKVLTPEEQLADKLRLKKLQEESDLELAKETFGVNNTVYGIDAMNPSSRDDFTEFGKLLKDKITQYEKSLYYASFLEALVRDVCISLEIDDLKKITNSLTVLCSEKQKQEKQSKAKKKKKGVVPGGGLKATMKDDLADYGGYDGGYVQDYEDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K7F5</accession>
    <name>A0A9L0K7F5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Multiple epidermal growth factor-like domains protein 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Epidermal growth factor-like protein 4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">MEGF8</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Acts as a negative regulator of hedgehog signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005041546.1">
        <property type="protein sequence ID" value="ENSEASP00005060763.1"/>
        <property type="gene ID" value="ENSEASG00005006253.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160262"/>
    <dbReference type="OMA" id="PVCQWCD"/>
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        <property type="component" value="Chromosome 26"/>
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    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035904">
        <property type="term" value="P:aorta development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030509">
        <property type="term" value="P:BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042074">
        <property type="term" value="P:cell migration involved in gastrulation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060976">
        <property type="term" value="P:coronary vasculature development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097094">
        <property type="term" value="P:craniofacial suture morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071907">
        <property type="term" value="P:determination of digestive tract left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061371">
        <property type="term" value="P:determination of heart left/right asymmetry"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990403">
        <property type="term" value="P:embryonic brain development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042733">
        <property type="term" value="P:embryonic digit morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060971">
        <property type="term" value="P:embryonic heart tube left/right pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003143">
        <property type="term" value="P:embryonic heart tube morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055113">
        <property type="term" value="P:epiboly involved in gastrulation with mouth forming second"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097155">
        <property type="term" value="P:fasciculation of sensory neuron axon"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045879">
        <property type="term" value="P:negative regulation of smoothened signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061626">
        <property type="term" value="P:pharyngeal arch artery morphogenesis"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0048842">
        <property type="term" value="P:positive regulation of axon extension involved in axon guidance"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0007224">
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        <property type="project" value="Ensembl"/>
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    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="4"/>
    </dbReference>
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        <property type="entry name" value="Laminin"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
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    <dbReference type="InterPro" id="IPR056737">
        <property type="entry name" value="Beta-prop_ATRN-MKLN-like"/>
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        <property type="entry name" value="EGF"/>
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    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
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    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
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    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
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    <dbReference type="InterPro" id="IPR002049">
        <property type="entry name" value="LE_dom"/>
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    <dbReference type="InterPro" id="IPR056863">
        <property type="entry name" value="LMN_ATRN_NET-like_EGF"/>
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    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002165">
        <property type="entry name" value="Plexin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF16">
        <property type="entry name" value="MULTIPLE EGF-LIKE-DOMAINS 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24981">
        <property type="entry name" value="Beta-prop_ATRN-LZTR1"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07645">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00053">
        <property type="entry name" value="EGF_laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24973">
        <property type="entry name" value="EGF_LMN_ATRN"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23106">
        <property type="entry name" value="EGF_Teneurin"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01437">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00011">
        <property type="entry name" value="EGFLAMININ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00180">
        <property type="entry name" value="EGF_Lam"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01248">
        <property type="entry name" value="EGF_LAM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50027">
        <property type="entry name" value="EGF_LAM_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0106">Calcium</keyword>
    <keyword evidence="11 18" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 18" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="2" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="13 19" id="KW-0424">Laminin EGF-like domain</keyword>
    <keyword evidence="10 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 22" id="KW-0732">Signal</keyword>
    <keyword evidence="5 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040296961" description="Multiple epidermal growth factor-like domains protein 8" evidence="22">
        <location>
            <begin position="28"/>
            <end position="2789"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="2592"/>
            <end position="2614"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="23">
        <location>
            <begin position="30"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="24">
        <location>
            <begin position="170"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="24">
        <location>
            <begin position="1074"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="25">
        <location>
            <begin position="1211"/>
            <end position="1261"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="23">
        <location>
            <begin position="1263"/>
            <end position="1405"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="24">
        <location>
            <begin position="1403"/>
            <end position="1445"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="24">
        <location>
            <begin position="2122"/>
            <end position="2160"/>
        </location>
    </feature>
    <feature type="domain" description="Laminin EGF-like" evidence="25">
        <location>
            <begin position="2197"/>
            <end position="2245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="2466"/>
            <end position="2507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="2765"/>
            <end position="2789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="2471"/>
            <end position="2484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="2488"/>
            <end position="2500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="2765"/>
            <end position="2775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="2780"/>
            <end position="2789"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="17">
        <location>
            <begin position="30"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="174"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="1233"/>
            <end position="1242"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="1245"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="18">
        <location>
            <begin position="1435"/>
            <end position="1444"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="2217"/>
            <end position="2226"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="2229"/>
            <end position="2243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00053684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005060763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="2789" mass="297260" checksum="F796DF09850C7231" modified="2023-09-13" version="1">MALGQALATALALSLAVLGPLSRGALAGDCKGQRQVLREAPGFVTDGAGNYSVNGNCEWLIEAPSPQHRILLDFLFLDTECTYDYLFVYDGDSPRGHLLASLSGSTRPPPIEASSGKMLLHLFSDANYNLLGFNASFRFSLCPGGCRSHGQCRPPGVCACEPGWGGPDCGLQECPAYCGSHGTCASPLGPCLCEPGFLGRACDLHLWENQGAGWWHNVSAGDPAFSARIGAAGAFLSPPGLLAVFGGQDLNRALGDLVLYNFSANTWERWDLSPAPAARHSHVAVAWAGSLVLMGGELADGSLTSDVWAFSPQGGGHWELLAPPVSSSLGPPGLAGHAAALVDDIWLYVSGGRTQHDLFSSGLFRFRLDSTSRGYWEQVIPAGGRPPAATGHSMVFHAPSRTLLVHGGHRPSTARFSVRVNSTELFHVDRRVWTTLKGRDGLQGPRERAFHTASVLGNYMVVYGGNVHTHYQEEKCYEDGIFFYHLGCHQWVSGAELAPPGTPEGRAAPPSGRYSHVAAVLGGSVLLVAGGYSGRPRGDLMAYKVPPFVFQAPAPDYHLDYCSMYTDHSVCSRDPECSWCQGACQAAPPPGTPSGACPAASCLGLGRLLGDCQACLAFSSPAAPPRGPGALGWCVHNESCLPRPEQARCRGEQISGTVGWWGPAPVFVTSLEACITQSFLPGLHLLTFQQPPNASQPDKVLIVRSTTITLTPSPETDVSLVYRGFIHPLLPGGPGGPGAEDVAVWARAQRLHVLARMARGPDTENMEEVGRWVAQQEKETRRLQRPGSARLFPLPGRGHKYAVEIRGQLNGSAGPGHSELTLLWDRTGVPGGSEISFFFLEPYRSSACASYSSCLGCLADQGCGWCLTSATCHLRQSGAHCGADGAGGSLLVLVPALCPLCEEHRDCHACTQDPFCEWHQSTSRKGDAACSRRGRGLGALKSPEECPPLCSQRLTCDDCLANSSQCAWCQSTHTCFLFAAYLARYPHGGCRGWDDSVHSEPRCRSCDRFLTCHECLQSHECGWCGNEDNPTLGRCLQGDFSGPLGGGNCSLWVGEGLGLSVALPARWAYARCPDVDECRLGLARCHLRATCLNTPLSYECHCQRGYQGDGVTHCSRTCLEDCGHGVCSGPPDFTCVCDLGWTSDLPPPTPAPGPPAPRCSRDCGCNFHSHCRKRGPGFCDECQDWTWGEHCERCRPGSFGNATGSGGCRPCQCNGHGDPRRGHCDNLSGLCFCQDHTEGAHCQLCSPGYYGDPRAGGSCFRECGGRALLTNVSSVALGSRRVGGLLPPGGGAARTGTGLSYCVWVVSATEVLQPCAPGTLCPPLTLTFSPDSSTPCTLSYVLAFDGFPRFLDTGVVQSDRSLIAAFCGQRRDRPLTVQALSGLLVLHWEANGSSSWGFNASVGSARCGSGGPGSCPVPQECVPQDEAAGAGLCRCPQGWAGPHCRMALCPENCNAHSGAGTCNQSLGVCICAEGFGGPDCATKLDGGQLVWETLMDSRLSADTASRFLHRLGHTMVEGPDATLWMFGGLGLPQGLLGNLYRYSVSERRWTQMLAGAEDGGPGPSPRSFHAAAYVPAGRGAMYLLGGLTAGGVTRDFWVLNLTTLQWRQEKVPQTVELPAVAGHTLTARRGLSLLLVGGYSPENGFNQQLLEYQLATGTWVSGAQSGTPPTGLYGHSAVYHEATDSLYVFGGFRFHVELAAPSPELYSLHCPDRTWSLLAPSQGAKPRPRLFHASALLGDTMVVLGGRSDPDEFSSDVLLYQVNCNAWLLPDLTRPASVGPPMEESVAHAVAAVGGRLYISGGFGGVALGRLLALTLPPDPCRLLPSPEACNQSGACTWCHRACLSGDQAHRLGCGDPPCSPMPRSPEECRRLRTCSECLARHPRTLQPGDGEASAPRCKWCTNCPEGACIGRNGSCTSENDCRINQREVFWAGNCSEAACGAADCEQCTREGKCMWTRQFKRTGETRRILSVQPTYDWTCFSHSLLNVSPMPVESSPPLPCPTPCHLLPNCTSCLDSKGADGGWQHCVWSSSLQQCLSPSYLPLRCMAGGCGRLLRGPESCSLGCAQASQCALCLRRPHCGWCAWGGQDGGGSCLEGGLSGPREGLTCGRPGTSWAFLSCPPEDECANGHHDCNETQNCHDRPHGYECSCKTGYTMDNVTGLCRPVCAQGCVNGSCVEPDHCRCHFGFVGRNCSTECRCNRHSECAGVGARDHCLLCRNHTKGSHCEQCLPLFVGSAVGGGTCRPCHAFCRGNSHVCVSRKELEMARREPEKYSLDPEEIENWVTEGPSEDEAVCVSCQNNSYGDKCESCLHGYFLLDGKCTKCQCNGHADTCNEQDGTGCPCQNNTETGTCQGSSPSDRRDCYKYQCAKCRESFHGSPLGGQQCYRLISVEQECCLDPTSQTNCFHEPKRRALGPGRTVLFGVQPKFTNVDIRLTLDVTFGAVDLYVSTSYDTFVVRVAPDTGVHTVHIQPPPPPPPPPPPADSGPRGTGDPGGPGPGTVPGTTAEPRVREVWPRGLITYVTVTEPSAVLVVRSVRDRLVITYPHEHHALKSSRFYLLLLGVGDPNGPGANGSADSQGLLFFRQDQAHIDLFVFFSVFFSCFFLFLSLCVLLWKAKQALDQRQEQRRHLQEMTKMASRPFAKVTVCFPPDPAAPAPTWKPAGLPPPTFRRSEPFLAPLLLTGAGGPWGPVGGGCCPPAIPTTTAGLRAGPITLEPTEDGMAGVATLLLQLPGGPHAPNGACLGSALVTLRHRLHEYCGGGGGAGGSGHGAGAGRKGLLSQDNLTSMSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K9Q8</accession>
    <name>A0A9L0K9Q8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Catechol O-methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Catechol O-methyltransferase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">TOMT</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Required for auditory function. Component of the cochlear hair cell's mechanotransduction (MET) machinery. Involved in the assembly of the asymmetric tip-link MET complex. Required for transportation of TMC1 and TMC2 proteins into the mechanically sensitive stereocilia of the hair cells. The function in MET is independent of the enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>2-hydroxy-17beta-estradiol + S-adenosyl-L-methionine = 2-hydroxy-3-methoxy-17beta-estradiol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28744"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:89268"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:53093"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>2-hydroxy-17beta-estradiol + S-adenosyl-L-methionine = 2-methoxy-17beta-estradiol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53088"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28744"/>
            <dbReference type="ChEBI" id="CHEBI:28955"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:53089"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>2-hydroxyestrone + S-adenosyl-L-methionine = 2-hydroxy-3-methoxy-estrone + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53108"/>
            <dbReference type="ChEBI" id="CHEBI:1156"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:136980"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:53109"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>2-hydroxyestrone + S-adenosyl-L-methionine = 2-methoxyestrone + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53100"/>
            <dbReference type="ChEBI" id="CHEBI:1156"/>
            <dbReference type="ChEBI" id="CHEBI:1189"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:53101"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>4-hydroxy-17beta-estradiol + S-adenosyl-L-methionine = 4-methoxy-17beta-estradiol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53096"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:62845"/>
            <dbReference type="ChEBI" id="CHEBI:136975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:53097"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>4-hydroxyestrone + S-adenosyl-L-methionine = 4-methoxyestrone + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53104"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:87602"/>
            <dbReference type="ChEBI" id="CHEBI:136972"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:53105"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>a catechol + S-adenosyl-L-methionine = a guaiacol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33566"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:134251"/>
            <dbReference type="EC" id="2.1.1.6"/>
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        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:17878"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
            <orientation evidence="2">Extracellular side</orientation>
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        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.</text>
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        <property type="component" value="Chromosome 20"/>
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        <property type="term" value="C:apical part of cell"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="P:auditory receptor cell development"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:catecholamine catabolic process"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:methylation"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:1904591">
        <property type="term" value="P:positive regulation of protein import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0007605">
        <property type="term" value="P:sensory perception of sound"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000054">
        <property type="entry name" value="Catechol O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43836">
        <property type="entry name" value="CATECHOL O-METHYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43836:SF1">
        <property type="entry name" value="TRANSMEMBRANE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9" id="KW-1009">Hearing</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-0531">Neurotransmitter degradation</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="7" id="KW-0808">Transferase</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00003256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012880"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016706"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022867"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022939"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00033047"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00045199"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047302"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047350"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047582"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00048257"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005058020.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="258" mass="28910" checksum="D2E70E44E50937A9" modified="2023-09-13" version="1">MSPAIALAFLPLVVTLLVRYRHYFRLLVRTVLLRSLRDCLSGLRIEERAFSYVLTHTLPGDPGHILTTLDHWSSHCEYLSHMGPIKGQILMRLVEEKAPASVLELGTYCAYSTLFIARALPPGGRLLTVERDPRTAAVAEKLIRLAGFDERTVELIVGRSEEVIPRLRTQYQLSRADLVLLAHRPRCYLRDLQLLEAHALLPAGATVLADHVLFPGAPRFLQYTKSCGRYRCRLHHTGLPDFPAIKDGIAQLTYAGPG</sequence>
</entry>
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    <accession>A0A9L0KBL1</accession>
    <name>A0A9L0KBL1_EQUAS</name>
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        <submittedName>
            <fullName evidence="4">ARB2 cotranscriptional regulator A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ARB2A</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_070517781.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0KBL1"/>
    <dbReference type="Ensembl" id="ENSEAST00005063130.1">
        <property type="protein sequence ID" value="ENSEASP00005062203.1"/>
        <property type="gene ID" value="ENSEASG00005019511.2"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 11"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035197">
        <property type="term" value="F:siRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048263">
        <property type="entry name" value="Arb2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053858">
        <property type="entry name" value="Arb2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21357:SF3">
        <property type="entry name" value="COTRANSCRIPTIONAL REGULATOR FAM172A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21357">
        <property type="entry name" value="FAM172 FAMILY PROTEIN HOMOLOG CG10038"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22749">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040357843" evidence="2">
        <location>
            <begin position="21"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="domain" description="Arb2" evidence="3">
        <location>
            <begin position="56"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005062203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="416" mass="48058" checksum="CB417C6B8C930A9F" modified="2023-09-13" version="1">MSISLSSLILLPIWINMSQIQQGGPDEKEKTTALKDLLSRIDLDELMKKDEPPLDFPDTLEGFEYAFNEKGQLRHIKTGEPFVFNYREDLHRWNQKRYEALGEIITKYVYDLLEKDCNLKKIFIPVDATESEPKSFIFMSEDALTNPQKLMVLIHGSGVVRAGQWARKLIINEDLDSGTQIPFIKRAMDEGYGVIVLNPNENCIEVEKPKTHVQSSSDSSDEPVEKRERKDKVFKETKKRRDFYEKYRNPQREKEMMQLYIRENGSPEEHTVYVWDHFIAKSAAENVFFVAHSYGGLAFVELMIQREADVKSKVTAVALTDSVHNVWHQEAGKTIREWMRENCCNWVSSSEPLDTSVESMLPDCPRVSAGTDRHELTSWKSLPSIFRFFTEASEAKTSSLRPAGTRRSHRLKPEEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KCH2</accession>
    <name>A0A9L0KCH2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Secretory carrier-associated membrane protein</fullName>
            <shortName evidence="18">Secretory carrier membrane protein</shortName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
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        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="Li"/>
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                <person name="Li"/>
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                <person name="Pan"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
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                <person name="M."/>
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                <person name="D.D."/>
                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="17">Interacts (via C-terminal part) with SYT1 and SYT2; interaction with synaptotagmins making a link with the SNARE molecules. Interacts with SLC9A7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cell membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle</location>
            <location evidence="3">Synaptic vesicle membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Membrane</location>
            <topology evidence="18">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16">Belongs to the SCAMP family. SCAMP5 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KCH2"/>
    <dbReference type="Ensembl" id="ENSEAST00005053490.1">
        <property type="protein sequence ID" value="ENSEASP00005063152.1"/>
        <property type="gene ID" value="ENSEASG00005033277.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157577"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:recycling endosome membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:synaptic vesicle membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:exocytosis"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:protein transport"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="SCAMP"/>
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        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN 5"/>
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    <dbReference type="PANTHER" id="PTHR10687">
        <property type="entry name" value="SECRETORY CARRIER-ASSOCIATED MEMBRANE PROTEIN SCAMP"/>
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        <property type="entry name" value="SCAMP"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9" id="KW-0967">Endosome</keyword>
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    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5 18" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004644"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038169"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038598"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363122"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="73" mass="8245" checksum="02F228C7C5B50CDC" modified="2023-09-13" version="1">MQCALPTEKVNNFPPLPKFIPLKPCFYQDFEADIPPQHLSMTKRLYYLWMCECCGMGVGQGGWGGGGRKVCRC</sequence>
</entry>
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    <name>A0A9L0KDS4_EQUAS</name>
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        <recommendedName>
            <fullName evidence="10">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
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            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="H."/>
                <person name="Guo"/>
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                <person name="Huang"/>
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                <person name="Liu"/>
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                <person name="Yang"/>
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                <person name="Tian"/>
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                <person name="Zhang"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="ENSEASG00005032514.1"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051117">
        <property type="entry name" value="TRG_var/const_region"/>
    </dbReference>
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        <property type="entry name" value="T CELL RECEPTOR GAMMA CONSTANT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19256">
        <property type="entry name" value="T-CELL RECEPTOR GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-0472">Membrane</keyword>
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    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040169420" description="Ig-like domain-containing protein" evidence="7">
        <location>
            <begin position="18"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005060632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="104" mass="11929" checksum="1EDCDB916C3E26C6" modified="2023-09-13" version="1">MLKALALLLAFLAPATQISPNLEGTQMSVTRQARSSVEITCDIKQSSDHIHWYRFQKRMAPRHLLYYQFSSSKFVESGTSSQKYRAYEGPGRTCKLYHQISGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KHG6</accession>
    <name>A0A9L0KHG6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase</fullName>
        </recommendedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
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                <person name="S."/>
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                <person name="Y."/>
                <person name="Liu"/>
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                <person name="Wang"/>
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                <person name="Zhuang"/>
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                <person name="Li"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
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        <text evidence="12">Interacts with ERBB4.</text>
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    <comment type="subcellular location">
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        <text evidence="3">Belongs to the neuregulin family.</text>
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    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KYH2"/>
    <dbReference type="Ensembl" id="ENSEAST00005002665.2">
        <property type="protein sequence ID" value="ENSEASP00005002421.2"/>
        <property type="gene ID" value="ENSEASG00005026540.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286921"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="CDD" id="cd15407">
        <property type="entry name" value="7tmA_OR5B-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
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    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
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    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 9" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0552">Olfaction</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="8 9" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="25"/>
            <end position="49"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="61"/>
            <end position="84"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="90"/>
            <end position="113"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="134"/>
            <end position="156"/>
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    <feature type="transmembrane region" description="Helical" evidence="10">
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="271"/>
            <end position="290"/>
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    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="11">
        <location>
            <begin position="39"/>
            <end position="288"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005002421.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="310" mass="34429" checksum="A6C01A0A37F9004C" modified="2023-09-13" version="2">VKNSTEVTQFILLGLTNAPELQLPLFITFTLIYIITLIGNLGIIMLILLNSHLHTPMYFFLSNLSLVDLCYSSAVTPTVMAGFITVDKVISYNACAAQMFFFVAFATVENYLLASMAYDRYAAVCKPLHYSTTMTTGVCACLTTGSYICGFLNASIHIGDTFSLSFCMSNVVHDFFCDIPAVMVLSCSYRHVSELVLVYVVSFNIFFALLVILISYVIIFITILKMHSASGYQKALSTCASHFTAVSIFYGTIIFMYLQPSSSHSMDTDKMASVFYSMIIPMLNPVVYSLRNKEVKVAFMKSLVRAGFMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KYW1</accession>
    <name>A0A8C4KYW1_EQUAS</name>
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        <submittedName>
            <fullName evidence="10">GDNF family receptor alpha 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">GFRA4</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GDNFR family.</text>
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    <dbReference type="RefSeq" id="XP_044604796.1">
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    <dbReference type="Ensembl" id="ENSEAST00005001280.2">
        <property type="protein sequence ID" value="ENSEASP00005001122.2"/>
        <property type="gene ID" value="ENSEASG00005000940.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106847671"/>
    <dbReference type="CTD" id="64096"/>
    <dbReference type="GeneTree" id="ENSGT00940000160491"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016167">
        <property type="term" value="F:glial cell-derived neurotrophic factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030279">
        <property type="term" value="P:negative regulation of ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007399">
        <property type="term" value="P:nervous system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="entry name" value="GDNF family receptor alpha 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.110">
        <property type="entry name" value="GDNF binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016017">
        <property type="entry name" value="GDNF/GAS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037193">
        <property type="entry name" value="GDNF_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003438">
        <property type="entry name" value="GDNF_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269:SF2">
        <property type="entry name" value="GDNF FAMILY RECEPTOR ALPHA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10269">
        <property type="entry name" value="GDNF RECEPTOR ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02351">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01316">
        <property type="entry name" value="GDNFRECEPTOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00907">
        <property type="entry name" value="GDNF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110035">
        <property type="entry name" value="GDNF receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040215441" evidence="8">
        <location>
            <begin position="26"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="9">
        <location>
            <begin position="29"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="GDNF/GAS1" evidence="9">
        <location>
            <begin position="120"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001122.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="272" mass="29162" checksum="4495C2703D02439C" modified="2023-09-13" version="2">MARCLGPLLLLLLLLLLLGSPSSEGRNRCVDAAEACTADALCQQLRTEYLARCLDRAAPGGCPRARCRRALRRFFARGPPALTHALLFCPCAGPACAERRRQTFVPACAFSGPGLAPPSCLAPLDACEHSRVCRPRLLAFQASCAPAPSAPDGCLPDQAPSCRRAYAGLVGTAITPNYVDNASARVAPWCDCGASGNRREECEAFRGLFTRNRCLDSAIQAFVSWWPPILQDQLDSLQDPGHSLLQVSSADEPPEGSSLFFMLPVLALQPLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KZ50</accession>
    <name>A0A8C4KZ50_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">S-adenosylmethionine synthase</fullName>
            <ecNumber evidence="11">2.5.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">MAT2A</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
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                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="11">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="11">Binds 1 potassium ion per subunit. The potassium ion interacts primarily with the substrate.</text>
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    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per subunit. The magnesium ions interact primarily with the substrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the AdoMet synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="11"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4KZ50"/>
    <dbReference type="SMR" id="A0A8C4KZ50"/>
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        <property type="protein sequence ID" value="ENSEASP00005001840.2"/>
        <property type="gene ID" value="ENSEASG00005001463.2"/>
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    <dbReference type="GeneTree" id="ENSGT00950000183185"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
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    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
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    <dbReference type="GO" id="GO:0048269">
        <property type="term" value="C:methionine adenosyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:identical protein binding"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:methionine adenosyltransferase activity"/>
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        <property type="term" value="P:cellular response to amino acid starvation"/>
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        <property type="term" value="P:cellular response to leukemia inhibitory factor"/>
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        <property type="term" value="P:cellular response to methionine"/>
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        <property type="term" value="P:negative regulation of TORC1 signaling"/>
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        <property type="term" value="P:protein hexamerization"/>
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        <property type="term" value="P:protein localization to lysosome"/>
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        <property type="term" value="P:protein-containing complex localization"/>
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        <property type="match status" value="3"/>
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        <property type="entry name" value="S_AdoMet_synth1"/>
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        <property type="entry name" value="ADOMET_SYNTHASE_CS"/>
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        <property type="entry name" value="S-AdoMet_synt_C"/>
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        <property type="entry name" value="S-AdoMet_synt_central"/>
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        <property type="entry name" value="S-AdoMet_synthetase"/>
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        <property type="entry name" value="metK"/>
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    </dbReference>
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        <property type="entry name" value="S-AdoMet_synt_M"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="MAT"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="S-adenosylmethionine synthetase"/>
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        <property type="entry name" value="ADOMET_SYNTHASE_1"/>
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        <property type="entry name" value="ADOMET_SYNTHASE_2"/>
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    </dbReference>
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    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="9 11" id="KW-0630">Potassium</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S-adenosylmethionine synthetase N-terminal" evidence="13">
        <location>
            <begin position="18"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosylmethionine synthetase central" evidence="14">
        <location>
            <begin position="129"/>
            <end position="250"/>
        </location>
    </feature>
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        <location>
            <begin position="252"/>
            <end position="385"/>
        </location>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00048344"/>
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        <source>
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            <dbReference type="RuleBase" id="RU004462"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
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</entry>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
                <person name="Y."/>
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                <person name="Liu"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Li"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <accession>A0A8C4L322</accession>
    <name>A0A8C4L322_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">BPI fold containing family B member 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">BPIFB4</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L322"/>
    <dbReference type="Ensembl" id="ENSEAST00005006612.2">
        <property type="protein sequence ID" value="ENSEASP00005006037.2"/>
        <property type="gene ID" value="ENSEASG00005004467.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01100000263546"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.20.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017943">
        <property type="entry name" value="Bactericidal_perm-incr_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051660">
        <property type="entry name" value="BPI_fold-BPI/LBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001124">
        <property type="entry name" value="Lipid-bd_serum_glycop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017942">
        <property type="entry name" value="Lipid-bd_serum_glycop_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46019:SF4">
        <property type="entry name" value="BPI FOLD-CONTAINING FAMILY B MEMBER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46019">
        <property type="entry name" value="BPI FOLD-CONTAINING FAMILY B MEMBER 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01273">
        <property type="entry name" value="LBP_BPI_CETP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02886">
        <property type="entry name" value="LBP_BPI_CETP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00328">
        <property type="entry name" value="BPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00329">
        <property type="entry name" value="BPI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55394">
        <property type="entry name" value="Bactericidal permeability-increasing protein, BPI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040105158" evidence="2">
        <location>
            <begin position="18"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="domain" description="Lipid-binding serum glycoprotein N-terminal" evidence="3">
        <location>
            <begin position="173"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="domain" description="Lipid-binding serum glycoprotein C-terminal" evidence="4">
        <location>
            <begin position="417"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006037.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="619" mass="65503" checksum="9C2A9FC1925E729C" modified="2023-09-13" version="2">MWTAWCVAALSVAAVCGVRQDTNTVLRVTKDVLSNAISGALQKSDALHSALREVPMGVGGVPYNDFHVREPPPKYTNGKQLGGNYKYGHIETNDNAAQLGGKYRYGEIPESDGSIRDVRNGDYRSAENAYGGHRGRGRYRSAEGAATVGRLHRRELRPGEIPPGVATGALGPGGLLGTGGMLAADGILSGQGGLLGGGGLLGDGGLLGGGGVLGVLGEGGILSTVQGITGLRIVELTLPRVSVRLLPGVGVYLSLYTRVAINGKSLIGFLDIAVEVNITAKVRLTMDRMGYPRLVIERCDTLLGGIKVKLLRGLLPNLVDNLVNRVLVNVLPDLLCPIVDVVLGLVNDQLGLVDSLVPLGILGSVQYTFSSLPLVTGEFLELDLNTLVGEAGGDLIDYPLGRPAMAPRQKMPELPPMGDNTNSQLAISANFLGSVLTLLQKQGALDIDITDGMFQELPPLTTSTLGALIPKVFQQYPESRPLTIRIQVPNPPSVTLQKDKALVKVFATSEIMVSQPKDVETTICLIDVDTELLATFSVEGDKLMIDAKLDKTSLNLRTSNVGNFDVGLMEVLVGKIFDLAFMPVMNAVLGSGVPLPKILNIDFSNADIDVLEDLLVLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L6W0</accession>
    <name>A0A8C4L6W0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">OR6N2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Cell membrane</location>
            <topology evidence="2 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L6W0"/>
    <dbReference type="Ensembl" id="ENSEAST00005004846.2">
        <property type="protein sequence ID" value="ENSEASP00005004421.2"/>
        <property type="gene ID" value="ENSEASG00005003340.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835801"/>
    <dbReference type="KEGG" id="eai:106835801"/>
    <dbReference type="CTD" id="81442"/>
    <dbReference type="GeneTree" id="ENSGT00940000158895"/>
    <dbReference type="OrthoDB" id="9975554at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15914">
        <property type="entry name" value="7tmA_OR6N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF227">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="200"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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            <dbReference type="RuleBase" id="RU363047"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50262"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005004421.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="317" mass="35707" checksum="A13ED475D69AC9BD" modified="2023-09-13" version="2">MEQHNRSSLAEFVLLGFPKVGHVRGWLFVLLLLAYLFTICGNLLIFLVIRLDAALHIPMYHFVSLLSFLELWYTAATTPKMLANLLSEKKTISFAGCLLQTYFFHSLGASECYLLTAMAYDRYLAICRPLHYPAIMTPTLCGRLAAGCWVCGFLCPISEVILVSQLPFCGYNEIQHIFCDFPPLLSLACKDTSTNVLVDFAINAFIILVTFLFVMISYGRIIVAVLKIKTSVGRKKAFSTCASHLIVVLIFFGSITFMYVRLKESYSLTLDRTLAVVYSVLTPLVNPVIYSLRNQELIKAIKRTIFQKQGRASPIHQ</sequence>
</entry>
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    <name>A0A8C4L7C0_EQUAS</name>
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        <submittedName>
            <fullName evidence="8">Fc alpha and mu receptor</fullName>
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        <name evidence="8" type="primary">FCAMR</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
                <person name="Q."/>
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                <person name="Yang"/>
                <person name="C."/>
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                <person name="Zhuang"/>
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                <person name="Gao"/>
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                <person name="Li"/>
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                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
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                <person name="G."/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
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        </subcellularLocation>
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        <property type="component" value="Chromosome 25"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Ig_sub"/>
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    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
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        <property type="entry name" value="HIGH AFFINITY IMMUNOGLOBULIN ALPHA AND IMMUNOGLOBULIN MU FC RECEPTOR"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
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    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="41"/>
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            <begin position="42"/>
            <end position="499"/>
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    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="412"/>
            <end position="434"/>
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        <location>
            <begin position="28"/>
            <end position="147"/>
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        <location>
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            <begin position="359"/>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="CTCK_2"/>
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    <keyword evidence="3 8" id="KW-0202">Cytokine</keyword>
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    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
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    <keyword evidence="4 8" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044536390" description="Gremlin" evidence="8">
        <location>
            <begin position="22"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="CTCK" evidence="11">
        <location>
            <begin position="73"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9 10">
        <location>
            <begin position="73"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="87"/>
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    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="97"/>
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        </location>
    </feature>
    <feature type="disulfide bond" evidence="9">
        <location>
            <begin position="101"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR037254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR037254-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS01225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005004311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="168" mass="19259" checksum="310BDCDA1082C8A5" modified="2022-01-19" version="1">MLWKLSLSLFLVAVLVKVAEARKNRPAGAIPSPYKDGSSNTSERWQHQIKEVLASSQEALVVTERKYLKSDWCKTQPLRQTVSEEGCRSRTILNRFCYGQCNSFYIPRHVKKEEESFQSCAFCKPQRVTSVLVELDCPGLDPPFRLKKIQKVKQCRCMSVNLSDSDKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LA73</accession>
    <name>A0A8C4LA73_EQUAS</name>
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        <recommendedName>
            <fullName evidence="11">Calcium-binding tyrosine phosphorylation-regulated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106822894</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
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                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="Liu"/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell projection</location>
            <location evidence="1">Cilium</location>
            <location evidence="1">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
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            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
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        <property type="gene ID" value="ENSEASG00005004560.2"/>
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    <dbReference type="KEGG" id="eai:106822894"/>
    <dbReference type="GeneTree" id="ENSGT00390000000444"/>
    <dbReference type="OMA" id="MCKNPVE"/>
    <dbReference type="OrthoDB" id="252964at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0035686">
        <property type="term" value="C:sperm fibrous sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048240">
        <property type="term" value="P:sperm capacitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12100">
        <property type="entry name" value="DD_CABYR_SP17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.890.10:FF:000005">
        <property type="entry name" value="calcium-binding tyrosine phosphorylation-regulated protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.890.10">
        <property type="entry name" value="cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038848">
        <property type="entry name" value="CABYR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003117">
        <property type="entry name" value="cAMP_dep_PK_reg_su_I/II_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047579">
        <property type="entry name" value="DD_CABYR_SP17"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15494">
        <property type="entry name" value="CALCIUM-BINDING TYROSINE PHOSPHORYLATION-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15494:SF0">
        <property type="entry name" value="CALCIUM-BINDING TYROSINE PHOSPHORYLATION-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02197">
        <property type="entry name" value="RIIa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00394">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47391">
        <property type="entry name" value="Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
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    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="12"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
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    </feature>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
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        </location>
    </feature>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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    <accession>A0A8C4LBV7</accession>
    <name>A0A8C4LBV7_EQUAS</name>
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        <recommendedName>
            <fullName evidence="4">17-beta-hydroxysteroid dehydrogenase type 6</fullName>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LBV7"/>
    <dbReference type="Ensembl" id="ENSEAST00005009607.2">
        <property type="protein sequence ID" value="ENSEASP00005008819.2"/>
        <property type="gene ID" value="ENSEASG00005006329.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162028"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008202">
        <property type="term" value="P:steroid metabolic process"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
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    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
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    <dbReference type="PANTHER" id="PTHR43313:SF11">
        <property type="entry name" value="RETINOL DEHYDROGENASE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43313">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE/REDUCTASE FAMILY 9C"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008819.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="118" mass="13852" checksum="33B45D30B2B82D5D" modified="2023-09-13" version="2">MWLYLAVLVGLYYLLRWYRERQVVSNLRDKYIFITGCDSGFGNQLARQLDLRGLRVLAACLTEKGAEQLRDRTSDRLETVILDVTKTESIAAATQWVKERTRDRVFYNLMGFFQNHIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LC97</accession>
    <name>A0A8C4LC97_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">TLE family member 3, transcriptional corepressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TLE3</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat Groucho/TLE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014703691.1">
        <property type="nucleotide sequence ID" value="XM_014848205.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LC97"/>
    <dbReference type="Ensembl" id="ENSEAST00005008218.2">
        <property type="protein sequence ID" value="ENSEASP00005007535.2"/>
        <property type="gene ID" value="ENSEASG00005005509.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835666"/>
    <dbReference type="KEGG" id="eai:106835666"/>
    <dbReference type="CTD" id="7090"/>
    <dbReference type="GeneTree" id="ENSGT01030000234519"/>
    <dbReference type="OrthoDB" id="2624652at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990907">
        <property type="term" value="C:beta-catenin-TCF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003714">
        <property type="term" value="F:transcription corepressor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120163">
        <property type="term" value="P:negative regulation of cold-induced thermogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000001">
        <property type="entry name" value="transducin-like enhancer protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005617">
        <property type="entry name" value="Groucho/TLE_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009146">
        <property type="entry name" value="Groucho_enhance"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10814">
        <property type="entry name" value="TRANSDUCIN-LIKE ENHANCER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10814:SF24">
        <property type="entry name" value="TRANSDUCIN-LIKE ENHANCER PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03920">
        <property type="entry name" value="TLE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01850">
        <property type="entry name" value="GROUCHOFAMLY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <keyword evidence="4" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="Groucho/TLE N-terminal Q-rich" evidence="11">
        <location>
            <begin position="18"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="582"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="624"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="139"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="195"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="26"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="219"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="294"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="310"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="320"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00045617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF03920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007535.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="782" mass="84494" checksum="F3F60BD12E6079F0" modified="2023-09-13" version="2">MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNTILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGVRGLPSLPFTQQQLQAQHLSHATHGPPVQLPPHPSGLQPPGIPPVTGSSSGLLALGALGGQAHLTVKDEKNHHELDHRERESSVNNSVSPSESLRASEKHRGSADYSMEAKKRKAEEKDSLSRYDSDGDKSDDLVVDVSNEDPATPRVSPAHSPPENGLDKARGLKKDAPTSPASVASSSSTPSSKTKDLGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRAMPGKPPGMDPIGIMASALRTPISITSSYAAPFAMMGHHEMNGSLTSPSAYAGLHNIPPQMSAAAAAAAAAYGRSPMVSFGAVGFDPHPPMRATGLPSSLASIPGGKPAYSFHVSADGQMQPVPFPHDALAGPNIPRHARQINTLSHGEVVCAVTISNPTRHVYTGGKGCVKIWDISQPGSKSPISQLDCLNRDNYIRSCKLLPDGRTLIVGGEASTLTIWDLASPTPRIKAELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTKPDKYQLHLHESCVLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGSGDKKATVYEVIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LD94</accession>
    <name>A0A8C4LD94_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Sodium-independent sulfate anion transporter</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Solute carrier family 26 member 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">SLC26A11</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Sodium-independent anion exchanger mediating bicarbonate, chloride, sulfate and oxalate transport. Exhibits sodium-independent sulfate anion transporter activity that may cooperate with SLC26A2 to mediate DIDS-sensitive sulfate uptake into high endothelial venules endothelial cells (HEVEC). In the kidney, mediates chloride-bicarbonate exchange, facilitating V-ATPase-mediated acid secretion. May function as a chloride channel, playing an important role in moderating chloride homeostasis and neuronal activity in the cerebellum.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>hydrogencarbonate(in) + chloride(out) = hydrogencarbonate(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:72363"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>oxalate(in) + chloride(out) = oxalate(out) + chloride(in)</text>
            <dbReference type="Rhea" id="RHEA:72263"/>
            <dbReference type="ChEBI" id="CHEBI:17996"/>
            <dbReference type="ChEBI" id="CHEBI:30623"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>sulfate(in) + H(+)(in) = sulfate(out) + H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:28574"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16189"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Apical cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Basolateral cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LD94"/>
    <dbReference type="Ensembl" id="ENSEAST00005007653.2">
        <property type="protein sequence ID" value="ENSEASP00005007008.2"/>
        <property type="gene ID" value="ENSEASG00005005138.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286920"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016324">
        <property type="term" value="C:apical plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008509">
        <property type="term" value="F:monoatomic anion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008271">
        <property type="term" value="F:secondary active sulfate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.750.24:FF:000013">
        <property type="entry name" value="Solute carrier family 26 member 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018045">
        <property type="entry name" value="S04_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01130">
        <property type="entry name" value="SLC26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0039">Anion exchange</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0458">Lysosome</keyword>
    <keyword evidence="11 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 19" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="76"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="308"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="380"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="407"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="440"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="20">
        <location>
            <begin position="480"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00050316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00052349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00054369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007008.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="606" mass="65085" checksum="841E84E1159FE618" modified="2023-09-13" version="2">MPSSVKALGQARPSGAGMAPQACCCSPAAVQRRLPILAWLPDYSMQWLKMDFIAGLSVGLTVIPQALAYAEVAGLPPQYGLYSAFMGCFVYFFLGTSKDVTLGPTAIMSLLVSCYTFHEPAYAVLLAFLSGCIQLAMGFLRLGFLLDFISCPVIKGFTSAAAVTIGFGQIKSLLGLQNIPRQFFLQVYHTFRNIGETRVGDAVLGLVCMVLLLVLKLMRDHVPPVHPEMPPGVRLSHGLVWTATTARNALVVSFAALVAYSFEVTGYQPFILTGEIAEGLPPVRTPPFSVTTANGTVSFTEMVQDMGAGLAVVPLMGLLESIAVAKSFASQNNYRIDANQELLAIGLTNMLGSLVSSYPVTGSFGRTAVNAQSGVCTPAGGLVTGVLVLLSLDYLTSLFYYIPKSALAAVIIMAVAPLFDTKIIGTLWRVKRLDLLPLCVTFLLCFWEVQYGILAGTLVSVLILLHSVARPKMQVSEGPVLVLQPVSGLHFPAVEALREAILSRALEASPPRCAVLECTHICSIDYTVVLGLGELLEDFHKQGVTLAFVGLQVPVLRVLLSADLKGVQYFSTLEEAEKYLKQEPGSQPYNISEDSVPEHKVALLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LDV5</accession>
    <name>A0A8C4LDV5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I, II, and III subunit RPABC5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">DNA-directed RNA polymerase III subunit L</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">POLR2L</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LDV5"/>
    <dbReference type="Ensembl" id="ENSEAST00005011466.2">
        <property type="protein sequence ID" value="ENSEASP00005010536.2"/>
        <property type="gene ID" value="ENSEASG00005007478.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000007087"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000024">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00250">
        <property type="entry name" value="RNApol_arch_Rpo10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023580">
        <property type="entry name" value="RNA_pol_su_RPB10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020789">
        <property type="entry name" value="RNA_pol_suN_Zn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000268">
        <property type="entry name" value="RPABC5/Rpb10"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003089">
        <property type="entry name" value="PRK04016.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431:SF11">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01194">
        <property type="entry name" value="RNA_pol_N"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF46924">
        <property type="entry name" value="RNA polymerase subunit RPB10"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS01112">
        <property type="entry name" value="RNA_POL_N_8KD"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00020813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00025720"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00054640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078853"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005010536.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="177" mass="18280" checksum="D2BE9EBB52EAFDCB" modified="2023-09-13" version="2">MRPRETRTAAAEPLRLRAAAVGRNVPKQQLGGAAGSRRGRAGPGTSDPRGGSSLRRAADAGGAGAGGAGWRRPGAGSATHPLLYGRVRGGAGATRGSAAGPRSASSSAAAMIIPVRCFTCGKIVGNKWEAYLGLLQAEYTEGDALDALGLKRYCCRRMLLAHVDLIEKLLNYAPLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LFX1</accession>
    <name>A0A8C4LFX1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Sodium/potassium-transporting ATPase subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">ATP1A3</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Cell membrane</location>
            <topology evidence="1 20">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 20">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_044760320.2"/>
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        <property type="gene ID" value="ENSEASG00005004546.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106847933"/>
    <dbReference type="KEGG" id="eai:106847933"/>
    <dbReference type="CTD" id="478"/>
    <dbReference type="GeneTree" id="ENSGT00940000160476"/>
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        <property type="component" value="Chromosome 26"/>
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    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043025">
        <property type="term" value="C:neuronal cell body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031090">
        <property type="term" value="C:organelle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005890">
        <property type="term" value="C:sodium:potassium-exchanging ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001540">
        <property type="term" value="F:amyloid-beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005391">
        <property type="term" value="F:P-type sodium:potassium-exchanging transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006883">
        <property type="term" value="P:intracellular sodium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990535">
        <property type="term" value="P:neuron projection maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd02608">
        <property type="entry name" value="P-type_ATPase_Na-K_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000106">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000001">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000004">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1110.10:FF:000095">
        <property type="entry name" value="Sodium/potassium-transporting ATPase subunit alpha-1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006068">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004014">
        <property type="entry name" value="ATPase_P-typ_cation-transptr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050510">
        <property type="entry name" value="Cation_transp_ATPase_P-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005775">
        <property type="entry name" value="P-type_ATPase_IIC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01106">
        <property type="entry name" value="ATPase-IIC_X-K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294">
        <property type="entry name" value="SODIUM/POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43294:SF15">
        <property type="entry name" value="SODIUM_POTASSIUM-TRANSPORTING ATPASE SUBUNIT ALPHA-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00689">
        <property type="entry name" value="Cation_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00690">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00121">
        <property type="entry name" value="NAKATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00831">
        <property type="entry name" value="Cation_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="17 20" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13 20" id="KW-0630">Potassium</keyword>
    <keyword evidence="5 20" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="16" id="KW-0915">Sodium</keyword>
    <keyword evidence="19" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="7" id="KW-0740">Sodium/potassium transport</keyword>
    <keyword evidence="14" id="KW-1278">Translocase</keyword>
    <keyword evidence="8 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 20" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 20" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="134"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="295"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="325"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="853"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="917"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="957"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="989"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="domain" description="Cation-transporting P-type ATPase N-terminal" evidence="22">
        <location>
            <begin position="46"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="15"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="SMART" id="SM00831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008100.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1027" mass="113088" checksum="7B570BBBE313028C" modified="2023-09-13" version="2">MGSGGSDSYRVATSQDKKDDKGSPKKNKGTKERRDLDDLKKEVAMTEHKMSVEEVCRKYNTDCVQGLTHSKAQEILARDGPNALTPPPTTPEWVKFCRQLFGGFSILLWIGAILCFLAYGIQAGTEDDPSGDNLYLGIVLAAVVIITGCFSYYQEAKSSKIMESFKNMVPQQALVIREGEKMQVNAEEVVVGDLVEIKGGDRVPADLRIISAHGCKVDNSSLTGESEPQTRSPDCTHDNPLETRNITFFSTNCVEGTARGVVVATGDRTVMGRIATLASGLEVGKTPIAIEIEHFIQLITGVAVFLGVSFFILSLILGYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTEDQSGTSFDKSSHTWVALSHIAGLCNRAVFKGGQDNIPVLKRDVAGDASESALLKCIELSSGSVKLMRERNKKVAEIPFNSTNKYQLSIHETEDPNDNRYLLVMKGAPERILDRCSTILLQGKEQPLDEEMKEAFQNAYLELGGLGERVLGFCHYYLPEEQFPKGFAFDCDDVNFTTDNLCFVGLMSMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVIHGTDLKDFTSEQIDEILQNHTEIVFARTSPQQKLIIVEGCQRQGAIVAVTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVEEGRLIFDNLKKSIAYTLTSNIPEITPFLLFIMANIPLPLGTITILCIDLGTDMVPAISLAYEAAESDIMKRQPRNPRTDKLVNERLISMAYGQIGMIQALGGFFSYFVILAENGFLPGNLVGIRLNWDDRTVNDLEDSYGQQWTYEQRKVVEFTCHTAFFVSIVVVQWADLIICKTRRNSVFQQGMKNKILIFGLFEETALAAFLSYCPGMDVALRMYPLKPSWWFCAFPYSFLIFVYDEIRKLILRRNPGGWVEKETYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LIR5</accession>
    <name>A0A8C4LIR5_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">DNA replication fork stabilization factor DONSON</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">DONSON</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DONSON family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LIR5"/>
    <dbReference type="Ensembl" id="ENSEAST00005009393.2">
        <property type="protein sequence ID" value="ENSEASP00005008617.2"/>
        <property type="gene ID" value="ENSEASG00005006187.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000447"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030894">
        <property type="term" value="C:replisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033260">
        <property type="term" value="P:nuclear DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024861">
        <property type="entry name" value="Donson"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12972">
        <property type="entry name" value="DOWNSTREAM NEIGHBOR OF SON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12972:SF0">
        <property type="entry name" value="PROTEIN DOWNSTREAM NEIGHBOR OF SON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02064">
        <property type="entry name" value="DONSON"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="329"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="44"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="132"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="329"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008617.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="558" mass="61611" checksum="0D25371F77F430A1" modified="2023-09-13" version="2">MALSVPGYSPSFKRPPETLRLRRKRARSRGAPSRPEPAPRRAALEAGLPLRPFPAAAAAGRGGGPAAAPRNPFARLDNRPRAAAEPPEEPARGQQEAPGPLLGANQENDLPREEKFPEGTAAPESLQTPHVSSSESDILSSGSTELPVDWSIKTRLLFTSSQPFTWADHLKAQEEAQGVVQHCRATEVTLPPSIQDPKLCTELRCAFQQSLVYWLHPAFPWLPLFPRIGADRKMAGKASPWSADEALQDILMSDWSVSFTSLYNLLKTKLCPYFYVCTYQFTILFRAAGLAGNDVITALISPTTRGLREAMKNEGIEFSLPLIKENGQEKKASRTSLGHEEEQAMSDEDEEESFSWLEEMGVQDKIKKPDILSIRLRKEKHEVQMDHRPESVVLVKGMNTFTLLNFLINCKSLIATSGPQAGLPPTLLSPIAFRGATMQMLKARSVNVKTQALSGYKDQFSLEITGPVMPHSLHSVTMLLKSSQSGSFSAGLYTHEPTAVFNICPPVTEVQDKETVHEELANCGLHPKTLDQLSQTPVLGKSSLRHVKMSDYIYNWRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LPC0</accession>
    <name>A0A8C4LPC0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Poly(U)-binding-splicing factor PUF60</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">60 kDa poly(U)-binding-splicing factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">PUF60</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM half pint family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044636918.1">
        <property type="nucleotide sequence ID" value="XM_044780983.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LPC0"/>
    <dbReference type="Ensembl" id="ENSEAST00005015117.2">
        <property type="protein sequence ID" value="ENSEASP00005013924.1"/>
        <property type="gene ID" value="ENSEASG00005009695.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106841890"/>
    <dbReference type="CTD" id="22827"/>
    <dbReference type="GeneTree" id="ENSGT00940000155594"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000380">
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        <property type="term" value="P:apoptotic process"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0006376">
        <property type="term" value="P:mRNA splice site recognition"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd12648">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="poly(U)-binding-splicing factor PUF60 isoform X1"/>
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        <property type="match status" value="3"/>
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    <dbReference type="InterPro" id="IPR006532">
        <property type="entry name" value="PUF60-like"/>
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        <property type="entry name" value="PUF60_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034209">
        <property type="entry name" value="PUF60_RRM1"/>
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    <dbReference type="InterPro" id="IPR034211">
        <property type="entry name" value="PUF60_RRM2"/>
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        <property type="entry name" value="PUF60_RRM3"/>
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    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
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        <property type="entry name" value="RRM_dom"/>
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    <dbReference type="InterPro" id="IPR003954">
        <property type="entry name" value="RRM_euk-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01645">
        <property type="entry name" value="half-pint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47330:SF1">
        <property type="entry name" value="POLY(U)-BINDING-SPLICING FACTOR PUF60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47330">
        <property type="entry name" value="POLY(U)-BINDING-SPLICING FACTOR PUF60-B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00361">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
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        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0678">Repressor</keyword>
    <keyword evidence="14" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 16" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RRM" evidence="18">
        <location>
            <begin position="129"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="18">
        <location>
            <begin position="226"/>
            <end position="304"/>
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    </feature>
    <feature type="domain" description="RRM" evidence="18">
        <location>
            <begin position="462"/>
            <end position="549"/>
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    <feature type="region of interest" description="Disordered" evidence="17">
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            <begin position="1"/>
            <end position="22"/>
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    <feature type="region of interest" description="Disordered" evidence="17">
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            <begin position="416"/>
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            <begin position="427"/>
            <end position="437"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005987"/>
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            <dbReference type="ARBA" id="ARBA00018665"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
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            <dbReference type="ARBA" id="ARBA00022491"/>
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            <dbReference type="ARBA" id="ARBA00022664"/>
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    <evidence type="ECO:0000256" key="6">
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            <dbReference type="PROSITE" id="PS50102"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="559" mass="59884" checksum="A0D782F58535D707" modified="2022-01-19" version="1">MASLLLPQVNGQQGGGSEPAAAAAAVVAAGDKWKPPQGTDSIKMENGQSTAAKLGLPPLTPEQQEALQKAKKYAMEQSIKSVLVKQTIAHQQQQLTNLQMAAVTMGFGDPLSPLQSMAAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLATPGLVSPALTLAQPLGALPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LPJ7</accession>
    <name>A0A8C4LPJ7_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Homeobox C5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">HOXC5</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the Antp homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LPJ7"/>
    <dbReference type="Ensembl" id="ENSEAST00005015840.2">
        <property type="protein sequence ID" value="ENSEASP00005014571.2"/>
        <property type="gene ID" value="ENSEASG00005010157.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161023"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030054">
        <property type="term" value="C:cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009952">
        <property type="term" value="P:anterior/posterior pattern specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048704">
        <property type="term" value="P:embryonic skeletal system morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000055">
        <property type="entry name" value="Homeobox protein Hox-A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050609">
        <property type="entry name" value="Antp_homeobox_Deformed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017995">
        <property type="entry name" value="Homeobox_antennapedia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001827">
        <property type="entry name" value="Homeobox_Antennapedia_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45771:SF13">
        <property type="entry name" value="HOMEOBOX C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45771">
        <property type="entry name" value="HOMEOTIC PROTEIN DEFORMED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00025">
        <property type="entry name" value="ANTENNAPEDIA"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00032">
        <property type="entry name" value="ANTENNAPEDIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="9 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="14">
        <location>
            <begin position="153"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="155"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="68"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="74"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="107"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
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    </evidence>
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    </evidence>
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    </evidence>
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    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014571.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="222" mass="25041" checksum="359FDEC18F6A97B9" modified="2023-09-13" version="2">MSSYVANSFYKQSPNIPAYYMQTCGNYGSASEVQASRYCYGGLDLSITFPPPAPSNSLHGVDMAANPRAHPDRPACSAAAAPGHALGRDEAAPLNPGMYSQKAARPALEERAKSSGEIKEEQAQTGQPAGLSQPPAPPQIYPWMTKLHMSHETDGKRSRTSYTRYQTLELEKEFHFNRYLTRRRRIEIANNLCLNERQIKIWFQNRRMKWKKDSKMKSKEAL</sequence>
</entry>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Zhao"/>
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                <person name="Wang"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8 9">Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers. Gamma-tubulin is a key component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation. The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the tubulin family.</text>
    </comment>
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    <dbReference type="SMR" id="A0A8C4LQW5"/>
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        <property type="component" value="Chromosome 13"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Tubulin gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002454">
        <property type="entry name" value="Gamma_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
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    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
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    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
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    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
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        <property type="entry name" value="Tubulin_C"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR01164">
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="10">
        <location>
            <begin position="48"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="11">
        <location>
            <begin position="249"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="451" mass="51079" checksum="B7109C1534B5C693" modified="2023-09-13" version="1">MPREIITLQLGQCGNQIGFEFWKQLCAEHGISPEGIVEEFATEGTDRKDVFFYQADDEHYIPRAVLLDLEPRVIHSILNSPYAKLYNPENIYLSEHGGGAGNNWASGFSQGEKIHEDIFDIIDREADGSDSLEGFVLCHSIAGGTGSGLGSYLLERLNDRYPKKLVQTYSVFPNQDEMSDVVVQPYNSLLTLKRLTQNADCVVVLDNTALNRIATDRLHIQNPSFSQINQLVSTIMSASTTTLRYPGYMNNDLIGLIASLIPTPRLHFLMTGYTPLTTDQSVASVRKTTVLDVMRRLLQPKNVMVSTGRDRQTNHCYIAILNIIQGEVDPTQVHKSLQRIRERKLANFIPWGPASIQVALSRKSPYLPSAHRVSGLMMANHTSISSLFESSCQQYDKLRKREAFLEQFRKEDIFKENFDELDRSREVVQELIDEYHAATRPDYISWGTQEQ</sequence>
</entry>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="J."/>
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                <person name="Zhang"/>
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                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="Zhuang"/>
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                <person name="Gao"/>
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                <person name="Ju"/>
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                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="G."/>
                <person name="Liu"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
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                <person name="Yin"/>
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                <person name="Ji"/>
                <person name="C."/>
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                <person name="Yue"/>
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                <person name="Wu"/>
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                <person name="X. and Zhong"/>
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            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the H-rev107 family.</text>
    </comment>
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        <property type="term" value="F:N-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0008970">
        <property type="term" value="F:phospholipase A1 activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:N-acylphosphatidylethanolamine metabolic process"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="HRAS like suppressor 2"/>
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        <property type="entry name" value="endopeptidase domain like (from Nostoc punctiforme)"/>
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    <dbReference type="InterPro" id="IPR051496">
        <property type="entry name" value="H-rev107_PLA/AT"/>
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    <dbReference type="InterPro" id="IPR007053">
        <property type="entry name" value="LRAT_dom"/>
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    <dbReference type="PANTHER" id="PTHR13943">
        <property type="entry name" value="HRAS-LIKE SUPPRESSOR - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13943:SF2">
        <property type="entry name" value="PHOSPHOLIPASE A AND ACYLTRANSFERASE 5"/>
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        <property type="entry name" value="LRAT"/>
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        <property type="entry name" value="LRAT"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="LRAT" evidence="6">
        <location>
            <begin position="118"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="86"/>
            <end position="114"/>
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        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="86"/>
            <end position="95"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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            <dbReference type="ARBA" id="ARBA00023098"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51934"/>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005017423.1"/>
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    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="262" mass="28713" checksum="AAEAB2C00DA89E63" modified="2022-01-19" version="1">MGLSRAPGGECRPSLSKFPRSRPKPASRTAGMGPNDSQPAPEESGDSQALVQLLPKQPEQGTLEQGRSIQQGEKVVVNLEITPNQKRAEWNSTPKPTRAGKLTKQAAKGKPKPRPGDLIEIFRIGYEHWAIYVEDDCVVHLAPPSEEFEAGSITSIFSNRAVVKYSRLEDVLHGCSWKINNKLDGTYLPLPVDNIIQRTKQMINKIVQYSLIEGNCEHFVNNLRYGVPRSQQVEHALMEGAKAAGAVISAVVGSMKPKPVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LZ97</accession>
    <name>A0A8C4LZ97_EQUAS</name>
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        <recommendedName>
            <fullName evidence="5">Vascular endothelial growth factor receptor 3</fullName>
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        </recommendedName>
        <alternativeName>
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        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Tyrosine-protein kinase receptor FLT4</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="38" type="primary">FLT4</name>
    </gene>
    <organism evidence="38 39">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="38 39" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="38" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="38" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="25 40">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.10.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="32">Membrane</location>
            <topology evidence="32">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="32">Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.</text>
    </comment>
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        <property type="gene ID" value="ENSEASG00005012804.2"/>
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        <property type="component" value="Chromosome 9"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019838">
        <property type="term" value="F:growth factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019903">
        <property type="term" value="F:protein phosphatase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005021">
        <property type="term" value="F:vascular endothelial growth factor receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048286">
        <property type="term" value="P:lung alveolus development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:lymphangiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of apoptotic process"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of endothelial cell migration"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of endothelial cell proliferation"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010575">
        <property type="term" value="P:positive regulation of vascular endothelial growth factor production"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060312">
        <property type="term" value="P:regulation of blood vessel remodeling"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:respiratory system process"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:sprouting angiogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:vascular endothelial growth factor receptor signaling pathway"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05862">
        <property type="entry name" value="IgI_VEGFR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05863">
        <property type="entry name" value="IgI_VEGFR-3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Vascular endothelial growth factor receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000532">
        <property type="entry name" value="Vascular endothelial growth factor receptor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000041">
        <property type="entry name" value="Vascular endothelial growth factor receptor 2"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:000143">
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        <property type="entry name" value="Vascular endothelial growth factor receptor 3"/>
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    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000411">
        <property type="entry name" value="Vascular endothelial growth factor receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000479">
        <property type="entry name" value="Vascular endothelial growth factor receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="vascular endothelial growth factor receptor 3"/>
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    <dbReference type="FunFam" id="2.60.40.10:FF:000949">
        <property type="entry name" value="vascular endothelial growth factor receptor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_I-set"/>
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    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
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    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
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    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050122">
        <property type="entry name" value="RTK"/>
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    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020635">
        <property type="entry name" value="Tyr_kinase_cat_dom"/>
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    <dbReference type="InterPro" id="IPR001824">
        <property type="entry name" value="Tyr_kinase_rcpt_3_CS"/>
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    <dbReference type="InterPro" id="IPR041348">
        <property type="entry name" value="VEGFR-2_TMD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055229">
        <property type="entry name" value="VEGFR1-3_5th"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055238">
        <property type="entry name" value="VEGFR1-3_N_Ig-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416">
        <property type="entry name" value="TYROSINE-PROTEIN KINASE RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24416:SF49">
        <property type="entry name" value="VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22971">
        <property type="entry name" value="Ig_VEGFR-1-like_5th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21339">
        <property type="entry name" value="VEGFR-1-like_Ig-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17988">
        <property type="entry name" value="VEGFR-2_TMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22854">
        <property type="entry name" value="VEGFR1-3_N_Ig-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="TyrPK_CSF1-R"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR01832">
        <property type="entry name" value="VEGFRECEPTOR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01835">
        <property type="entry name" value="VEGFRECEPTR3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00219">
        <property type="entry name" value="TyrKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00240">
        <property type="entry name" value="RECEPTOR_TYR_KIN_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="16 29 31" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="20" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="22" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="24 32" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="15" id="KW-0418">Kinase</keyword>
    <keyword evidence="30" id="KW-0460">Magnesium</keyword>
    <keyword evidence="18 34" id="KW-0472">Membrane</keyword>
    <keyword evidence="30" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 29 31" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21 32" id="KW-0675">Receptor</keyword>
    <keyword evidence="39" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0677">Repeat</keyword>
    <keyword evidence="12 35" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="11 32 34" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="17 34" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="19" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="signal peptide" evidence="35">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040192569" description="Vascular endothelial growth factor receptor 3" evidence="35">
        <location>
            <begin position="21"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="34">
        <location>
            <begin position="779"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="37">
        <location>
            <begin position="222"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="37">
        <location>
            <begin position="425"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="37">
        <location>
            <begin position="558"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="37">
        <location>
            <begin position="681"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="36">
        <location>
            <begin position="848"/>
            <end position="1176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="33">
        <location>
            <begin position="1291"/>
            <end position="1366"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="28">
        <location>
            <position position="1040"/>
        </location>
    </feature>
    <feature type="binding site" evidence="29">
        <location>
            <begin position="855"/>
            <end position="862"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="29 31">
        <location>
            <position position="882"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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        <location>
            <position position="1044"/>
        </location>
        <ligand>
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        </ligand>
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        <location>
            <position position="1045"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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        <location>
            <position position="1058"/>
        </location>
        <ligand>
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    <evidence type="ECO:0000256" key="1">
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022258"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022657"/>
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    </evidence>
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    </evidence>
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    </evidence>
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            <taxon>Metazoa</taxon>
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            <taxon>Eutheria</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
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                <person name="Jin"/>
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                <person name="Yue"/>
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                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="33"/>
            <end position="67"/>
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        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005018410.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="412" mass="48804" checksum="445E7D1B7B5E807B" modified="2023-09-13" version="2">MENTAKANKKDVQNGPIKEVKLPTSEALLEYYCQIKESAVERYMVQIKKLREKNQKYHERNKRLKDEQGWHLRNLLKELSEAKSEGLPVVTREEVEDAMKEKWNFERDQEQNLKDMRMQISNAEKLFLEKLSEKEYWEEYKNVGSEQHAKLIISLQNDINTVKENAEKMSEQYKITLEDARKRIIRETLLQLDQKKEWATENAIKFIDKGSCREIWENDWLKKEIANHKKEVEELENTIYELEEENLVLIEQLFNCRLVDLKIPRRLYLTQAAGLEVPPEEMPLEFPETYIGKAKSQPTEVKRRDTMSSSAGSSALHVSHEDSIQYGQYPKIDEKEISCIEFGASDMKYLLYEDERDFKDYVKLGPLEVKLMSVQSKKMPIHFQEKEMPVKFCEDVRIPESHITYKMMKSFL</sequence>
</entry>
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    <accession>A0A8C4M1E9</accession>
    <name>A0A8C4M1E9_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Zinc finger protein 234</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ZNF234</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4M1E9"/>
    <dbReference type="Ensembl" id="ENSEAST00005021473.2">
        <property type="protein sequence ID" value="ENSEASP00005019786.2"/>
        <property type="gene ID" value="ENSEASG00005013627.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163462"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000709">
        <property type="entry name" value="GDNF-inducible zinc finger protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001313">
        <property type="entry name" value="Zinc finger protein 155"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000274">
        <property type="entry name" value="zinc finger protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001158">
        <property type="entry name" value="zinc finger protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002239">
        <property type="entry name" value="Zinc finger protein 226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001534">
        <property type="entry name" value="zinc finger protein 227 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000623">
        <property type="entry name" value="Zinc finger protein 234"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002175">
        <property type="entry name" value="Zinc finger protein 234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000358">
        <property type="entry name" value="zinc finger protein 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000522">
        <property type="entry name" value="zinc finger protein 285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002153">
        <property type="entry name" value="Zinc finger protein 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002331">
        <property type="entry name" value="Zinc finger protein 672"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000624">
        <property type="entry name" value="zinc finger protein 697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000188">
        <property type="entry name" value="Zinc finger protein 787"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000028">
        <property type="entry name" value="zinc finger protein 90 homolog"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
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    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
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    <dbReference type="InterPro" id="IPR050527">
        <property type="entry name" value="Snail/Krueppel_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388:SF96">
        <property type="entry name" value="GENE, 32687-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24388">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="18"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="19"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="8"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="117"/>
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    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
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    <feature type="domain" description="C2H2-type" evidence="13">
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    <feature type="domain" description="C2H2-type" evidence="13">
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            <begin position="425"/>
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    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="453"/>
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    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="481"/>
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    <feature type="domain" description="C2H2-type" evidence="13">
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            <begin position="621"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
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            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
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    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
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    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M233</accession>
    <name>A0A8C4M233_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ORC ubiquitin ligase 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">OBI1</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>NAD(+) + ATP = ADP + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18629"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.23"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NAD kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.23" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4M5G3"/>
    <dbReference type="Ensembl" id="ENSEAST00005023262.2">
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        <property type="gene ID" value="ENSEASG00005014681.2"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:protein homodimerization activity"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:NAD+ metabolic process"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:NADP+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.30:FF:000027">
        <property type="entry name" value="NAD kinase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000021">
        <property type="entry name" value="NAD kinase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.30">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017437">
        <property type="entry name" value="ATP-NAD_kinase_PpnK-typ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002504">
        <property type="entry name" value="NADK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13158">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13158:SF5">
        <property type="entry name" value="NAD KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01513">
        <property type="entry name" value="NAD_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-0520">NAD</keyword>
    <keyword evidence="10" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="69"/>
            <end position="93"/>
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    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="117"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="117"/>
            <end position="133"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    <evidence type="ECO:0000256" key="7">
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            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
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            <dbReference type="ARBA" id="ARBA00023027"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
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            <dbReference type="ARBA" id="ARBA00068714"/>
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    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00077019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00080688"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    <evidence type="ECO:0000313" key="20">
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            <dbReference type="Ensembl" id="ENSEASP00005021428.1"/>
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    <evidence type="ECO:0000313" key="21">
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            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M6Z6</accession>
    <name>A0A8C4M6Z6_EQUAS</name>
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        <recommendedName>
            <fullName evidence="16">Transforming growth factor beta receptor type 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Betaglycan</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">Transforming growth factor beta receptor III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">TGFBR3</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
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            <taxon>Mammalia</taxon>
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    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subunit">
        <text evidence="15">Forms homodimers and homooligomers. Interacts with DYNLT4. Interacts with integrin ITGA5:ITGB1; this interaction promotes the internalization and trafficking of ITGA5:ITGB1 into endocytic vesicles. Interacts with TGFB1, BMP2, BMP5, BMP7 or GDF5 and inhibin A via the ligand binding domains. Interacts with ALK3/BMPR1A; this interaction results in the cell surface retention of BMPR1A. Interacts with ALK6/BMPR1B; this interaction enhances BMPR1B-mediated stimulation of the BMP signaling pathway. Interacts with the scaffolding protein beta-arrestin2/ARRB2; this interaction mediates internalization of TGFBR3 and thus regulates migration, actin cytoskeleton and activation of CDC42.</text>
    </comment>
    <comment type="subcellular location">
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            <topology evidence="1">Single-pass type I membrane protein</topology>
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            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
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        <property type="gene ID" value="ENSEASG00005015486.2"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0034673">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043235">
        <property type="term" value="C:receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036122">
        <property type="term" value="F:BMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015026">
        <property type="term" value="F:coreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005539">
        <property type="term" value="F:glycosaminoglycan binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046332">
        <property type="term" value="F:SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050431">
        <property type="term" value="F:transforming growth factor beta binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070123">
        <property type="term" value="F:transforming growth factor beta receptor activity, type III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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    <accession>A0A8C4MIU1</accession>
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            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Axon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the tenascin family. Teneurin subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005029562.2">
        <property type="protein sequence ID" value="ENSEASP00005027228.2"/>
        <property type="gene ID" value="ENSEASG00005018486.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234566"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 27"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007157">
        <property type="term" value="P:heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048666">
        <property type="term" value="P:neuron development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000016">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000021">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000026">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000003">
        <property type="entry name" value="Teneurin transmembrane protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.180.10.10:FF:000004">
        <property type="entry name" value="Teneurin transmembrane protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000013">
        <property type="entry name" value="Teneurin transmembrane protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.30:FF:000005">
        <property type="entry name" value="Teneurin transmembrane protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000008">
        <property type="entry name" value="teneurin-3 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.25.10">
        <property type="entry name" value="Laminin"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.180.10.10">
        <property type="entry name" value="RHS repeat-associated core"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.30">
        <property type="entry name" value="TolB, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011042">
        <property type="entry name" value="6-blade_b-propeller_TolB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008969">
        <property type="entry name" value="CarboxyPept-like_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057627">
        <property type="entry name" value="FN-plug_TEN1-4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022385">
        <property type="entry name" value="Rhs_assc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056823">
        <property type="entry name" value="TEN-like_YD-shell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009471">
        <property type="entry name" value="Ten_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056822">
        <property type="entry name" value="TEN_NHL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056820">
        <property type="entry name" value="TEN_TTR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051216">
        <property type="entry name" value="Teneurin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057629">
        <property type="entry name" value="Teneurin1-4_GBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028916">
        <property type="entry name" value="Tox-GHH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006530">
        <property type="entry name" value="YD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03696">
        <property type="entry name" value="Rhs_assc_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01643">
        <property type="entry name" value="YD_repeat_2x"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219">
        <property type="entry name" value="TENEURIN AND N-ACETYLGLUCOSAMINE-1-PHOSPHODIESTER ALPHA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11219:SF65">
        <property type="entry name" value="TENEURIN-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25024">
        <property type="entry name" value="EGF_TEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24329">
        <property type="entry name" value="FN-plug_TEN1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23093">
        <property type="entry name" value="GBD_Tenm3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06484">
        <property type="entry name" value="Ten_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25021">
        <property type="entry name" value="TEN_NHL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25023">
        <property type="entry name" value="TEN_YD-shell"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23538">
        <property type="entry name" value="Teneurin_ABD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15636">
        <property type="entry name" value="Tox-GHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25020">
        <property type="entry name" value="TTR_TEN1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49464">
        <property type="entry name" value="Carboxypeptidase regulatory domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101898">
        <property type="entry name" value="NHL repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51361">
        <property type="entry name" value="TENEURIN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="14" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12 19" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 19" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 21" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="21">
        <location>
            <begin position="153"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="Teneurin N-terminal" evidence="23">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="454"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="488"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="domain" description="EGF-like" evidence="22">
        <location>
            <begin position="583"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="476"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="511"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="587"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="19">
        <location>
            <begin position="608"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00077040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00081435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00083960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005027228.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="2543" mass="283304" checksum="FE28F704A2F838FF" modified="2023-09-13" version="2">MDVCERKGESIGCREQPANNQGQSTLQPLPPSHKQHSAQHHPSITSLNRNSLTNRRNQSPAPPAALPAELQTTPESVQLQDSWVLGSNVPLESRHFLFKTGTGTTPLFSTATPGYTMASGSVYSPPTRPLPRNTLSRSAFKFKKSSKYCSWKCTALCAVGVSVLLAILLSYFIAMHLFGLNWQLQQTENDTFENGKVNSDTMPTNTVSLPSGDNGKLGGFTQENNTIDSGELDIGRRAIQEVPPGIFWRSQLFIDQPQFLKFNISLQKDALIGVYGRKGLPPSHTQYDFVELLDGSRLIAREQRNLLESERAGRQARSVSLHEAGFIQYLDSGIWHLAFYNDGKNAEQVSFNTIVIESVVECPRNCHGNGECVSGTCHCFPGFLGPDCSRAACPVLCSGNGQYSKGRCLCFSGWKGTECDVPTTQCIDPLCGGRGICIMGSCACNSGYKGENCEEADCLDPGCSNHGVCIHGECHCSPGWGGNNCEILKTMCPDQCSGHGTYLQESGSCTCDPNWTGPDCSNEICSVDCGSHGVCMGGTCRCEEAWTGPACNQRACHPRCAEHGTCKDGKCECSQGWNGEHCTIEGCPGLCNSNGRCTLDQNGWHCVCQPGWRGAGCDVAMETLCTDSKDNEGDGLVDCMDPDCCLQSSCQNQPYCRGLPDPQDIISQSLQSPSQQAAKSFYDRISFLIGSDSTHVIPGESPFNKSLASVIRGQVLTADGTPLIGVNVSFFHYPEYGYTITRQDGMFDLVANGGASLTLVFERSPFLTQYHTVWIPWNVFYVMDTLVMKKEENDIPSCDLSGFVRPNPIIVSSPLSTFFRSSPEDSPIIPETQVLHEETTIPGTDLKLSYLSSRAAGYKSVLKITMTQSVIPFNLMKVHLMVAVVGRLFQKWFPASPNLAYTFIWDKTDAYNQKVYGLSEAVVSVGYEYESCLDLTLWEKRTAILQGYELDASNMGGWTLDKHHVLDVQNGILYKGNGENQFISQQPPVVSSIMGNGRRRSISCPSCNGQADGNKLLAPVALACGIDGSLYVGDFNYVRRIFPSGNVTSVLELSSNPAHRYYLATDPVTGDLYVSDTNTRRIYRPKSLTGAKDLTKNAEVVAGTGEQCLPFDEARCGDGGKAVEATLMSPKGMAIDKNGLIYFVDGTMIRKVDQNGIISTLLGSNDLTSARPLTCDTSMHISQVRLEWPTDLAINPMDNSIYVLDNNVVLQITENRQVRIAAGRPMHCQVPGVEYSVGKHAVQTTLESATAIAVSYSGVLYITETDEKKINRIRQVTTDGEISLVAGIPSECDCKNDANCDCYQSGDGYAKDAKLNAPSSLAASPDGTLYIADLGNIRIRAVSKNKPSLNSVNFYEVASPTDQELYIFDINGTHQYTVSLVTGDYLYNFSYSNDNDITAVTDSNGNTLRIRRDPNRMPVRVVSPDNQVIWLTIGTNGCLKSMTAQGLELVLFTYHGNSGLLATKSDETGWTTFFDYDSEGRLTNVTFPTGVVTNLHGDMDKAITVDIESSSRDEDVSITSNLSSIDSFYTMVQDQLRNSYQIGYDGSLRIIYASGLDSHYQTEPHVLAGTANPTVAKRNMTLPGENGQNLVEWRFRKEQAQGKVNVFGRKLRVNGRNLLSVDFDRTTKTEKIYDDHRKFLLRIAYDMSGHPTLWLPSSKLMAVNVTYSSTGQIASIQRGTTSEKVDYDSQGRIVSRVFADGKTWSYTYLEKSMVLLLHSQRQYIFEYDMWDRLSAITMPSVARHTMQTIRSIGYYRNIYNPPESNASVITDYNEEGLLLQTAFLGTSRRVLFKYRRQTRLSEILYDSTRVSFTYDETAGVLKTVNLQSDGFICTIRYRQIGPLIDRQIFRFSEDGMVNARFDYSYDNSFRVTSMQGVINETPLPIDLYQFDDISGKVEQFGKFGVIYYDINQIISTAVMTYTKHFDAHGRIKEIQYEIFRSLMYWITIQYDNMGRVTKREIKIGPFANTTKYAYEYDVDGQLQTVYLNEKIMWRYNYDLNGNLHLLNPSNSARLTPLRYDLRDRITRLGDVQYRLDEDGFLRQRGTEIFEYSSKGLLTRVYSKGSGWTVIYRYDGLGRRVSSKTSLGQHLQFFYADLTYPTRITHVYNHSSSEITSLYYDLQGHLFAMEISSGDEFYIASDNTGTPLAVFSSNGLMLKQIQYTAYGEIYFDSNIDFQLVIGFHGGLYDPLTKLIHFGERDYDILAGRWTTPDIEIWKRIGKDPAPFNLYMFRNNNPASKIHDVKDYISDVNSWLVTFGFHLHNAIPGFPVPKFDLTEPSYELVKSQQWDDIPPIFGVQQQVARQAKAFLSLGKMAEVQVSRRKGGGAQSWLWFATVKSLIGKGVMLAVSQGRVQTNVLNIANEDCIKVAAVLNNAFYLENLHFTIEGKDTHYFIKTTTPESDLGTLRLTSGRKALENGINVTVSQSTTVVNGRTRRFADVEMQFGALALHVRYGMTLDEEKARILEQARQRALARAWAREQQRVRDGEEGARLWTEGEKRQLLSAGKVQGYDGYYVLSVEQYPELADSANNIQFLRQSEIGKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4ML34</accession>
    <name>A0A8C4ML34_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Inter-alpha-trypsin inhibitor heavy chain 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ITIH2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ITIH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4ML34"/>
    <dbReference type="Ensembl" id="ENSEAST00005030751.2">
        <property type="protein sequence ID" value="ENSEASP00005028318.2"/>
        <property type="gene ID" value="ENSEASG00005019240.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157945"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004867">
        <property type="term" value="F:serine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006953">
        <property type="term" value="P:acute-phase response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030212">
        <property type="term" value="P:hyaluronan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01461">
        <property type="entry name" value="vWA_interalpha_trypsin_inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000013">
        <property type="entry name" value="inter-alpha-trypsin inhibitor heavy chain H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010600">
        <property type="entry name" value="ITI_HC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050934">
        <property type="entry name" value="ITIH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013694">
        <property type="entry name" value="VIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10338">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10338:SF14">
        <property type="entry name" value="INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06668">
        <property type="entry name" value="ITI_HC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08487">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00609">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51468">
        <property type="entry name" value="VIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0646">Protease inhibitor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0722">Serine protease inhibitor</keyword>
    <keyword evidence="5 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040108353" evidence="8">
        <location>
            <begin position="19"/>
            <end position="935"/>
        </location>
    </feature>
    <feature type="domain" description="VIT" evidence="10">
        <location>
            <begin position="45"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="9">
        <location>
            <begin position="299"/>
            <end position="482"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005028318.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="935" mass="105367" checksum="B6379A2EEDF960C4" modified="2023-09-13" version="2">MKRLTCFLIWFFVSEALGFEIPTNDLPEFAEYGDLVELTPRKFQFVPENRRYQEDVDQVTLHSYKVRSTITSRTANTVIQTKVVNNSPQPQNVVFDVQIPKGAFISNFSMTVDGTTFTSSIKEKSVGRALYSQARAKGKTAGLVRSRALDMEDFKTEVNILPGAKVQFELHYQEVKWRKLGSYEHRLHLQPGRLAKHLEVDVWIIEPQGLRFVHVPDTFDGHFDGVPVISKGPQKAHVSFKPTVAQQRKCSNCLETAVDGELVVLYDVNREEKAGELEVFNGYFVHFFAPDNMDPIPKNILFVIDVSGSMWGIKMKQTVEAMKTILDDLRTEDQFSVVDFNHNVRTWRNDLVSATTTQVADAKKYIEKIQPSGGTNINEALLRAIFILNEANNLGLLDPNSVSLIILVSDGDPTVGELKLSKIQKNVKQNIQDNISLFSLGIGFDVDYDFLKRLSNENRGIAHRIYGNQDTSSQLKKFYNQVSTPLLRNIQFNYPHTSVTDVTQNSFPNYFGGSEIVVAGKFHPEKLEQLQGIITVTSANTELVLETLAEMDDLEDFLSKDKHADPDFTKKLWAYLTINQLLAERSLAPTAAAKRKITKTILQMSLDHHIVTPLTSMVIENEAGDERMLADSPPQDHSCCSGALYYGSKVAPGSIPSWVNPSPTPMIPLPVKGAQVLESTPPPHVMRVENDPHFIIYLPKSQENICFNIDSEPGKILSLVSDPESGILVNGQLIGAKKTKNEKLSTYFGKLGFYFQSEDMKIEISTETITLSWGSRASTLSWADTARVINQRVLVSVKKEKTVTVTLDKKMSFSVLLHRVWKKHPINVDFLGIYIPPTNKFSPNVHGLIGQFMNEPKIHIFNERLGKDLKKPEASMEVKGHKLIVTRGLQKDYRTDIVFGTDVPCWFVHDSGKGFIDGHYKDYFVPQLYSFLKRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MRB5</accession>
    <name>A0A8C4MRB5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Kinesin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">KIF20A</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="16 17">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MRB5"/>
    <dbReference type="Ensembl" id="ENSEAST00005033052.2">
        <property type="protein sequence ID" value="ENSEASP00005030402.2"/>
        <property type="gene ID" value="ENSEASG00005020674.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106839208"/>
    <dbReference type="KEGG" id="eai:106839208"/>
    <dbReference type="CTD" id="10112"/>
    <dbReference type="GeneTree" id="ENSGT00940000156931"/>
    <dbReference type="OrthoDB" id="2403182at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032154">
        <property type="term" value="C:cleavage furrow"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045171">
        <property type="term" value="C:intercellular bridge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030496">
        <property type="term" value="C:midbody"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001578">
        <property type="term" value="P:microtubule bundle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061952">
        <property type="term" value="P:midbody abscission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051231">
        <property type="term" value="P:spindle elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01368">
        <property type="entry name" value="KISc_KIF23_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21787">
        <property type="entry name" value="RBD_KIF20A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000094">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000095">
        <property type="entry name" value="Kinesin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047149">
        <property type="entry name" value="KIF11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970:SF29">
        <property type="entry name" value="KINESIN FAMILY MEMBER 20B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47970">
        <property type="entry name" value="KINESIN-LIKE PROTEIN KIF11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8 16 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 17" id="KW-0493">Microtubule</keyword>
    <keyword evidence="13 16 17" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7 16 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Kinesin motor" evidence="20">
        <location>
            <begin position="64"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="659"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="825"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="18">
        <location>
            <begin position="560"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="662"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="160"/>
            <end position="167"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00055972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030402.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="891" mass="100214" checksum="BD535ADC5DFE3F9A" modified="2023-09-13" version="2">MSQGILSPPAGLLSDEEVVVSPMFESTAADLGSVVRKDLLSDCSVISASLEDKQQAPSEDSTEKVKVYLRVRPLLPSELERQEDQGCVHIENVETLVLQAPKDSFAQKSNERGIGQATHRFTFSQIFGPEVGQASFFNLTVKEMVKDVLKGQNWLIYAYGVTNSGKTHTIQGTIKDGGILPRSLALIFNSLQGQLHPTPDLKPLLSNEVIWLDSKQIRQQETKKLALLNGGLQEEELSTSLKKSVYIESRMGTSTSFDSGIAGLSSTSQFTSSSQLDETNHRWAQPDTAPVSVPADIRFSIWISFFEIYNELLYDLLEPPSQQRKRQTLRLCEDQNGNPYVKDLNWIHVQDAEEAWKLLKVGRKNQSFASTHLNQNSSRSHSIFSIRILHLQGEGDMVPKISELSLCDLAGSERCKDQKSGERLKEAGNINTSLHTLGRCIAALRQNQQNRSKQNLVPFRDSKLTRVFQGFFTGRGRSCMIVNVNPCASTYDETLHVAKFSAIASQLVHAPPVQLGFPLLHSFIKEPSLQASPSLQTGAKTDPGLDDDIENEVDISMYGKEELLQVVEAMKALLLKERQEKLQLEIQLRDEICNEMVEQMQQREQWCSEHLDTQKELLEEMYEEKLKILKESLTSFYQEELQERDEKIEELEALLQEAKQQSMAHQQSGSELSLRRSQRLAASTSTQQVQEVKAKLEQCRAELNSTNEELRKYQKMLEPPPSAKPFTTDVDKKLEEGQKNIRLLRTELQKLGESLQSAERACCHSTGAGKLRQALTSCDDILIKQDQTLAELQNNMMLVKLDLRKKAACIAEQYHTVLKLQGQASSTKKRLGANQENQQPNQQPPGKKPFLRNLLPRTPTCQSSTDCSPYARILRSRCSPLLKSGPFGKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MS62</accession>
    <name>A0A8C4MS62_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Interleukin-7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">IL7</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis. Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG. Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Interacts with IL7R and CSF2RG.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IL-7/IL-9 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044621846.1">
        <property type="nucleotide sequence ID" value="XM_044765911.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MS62"/>
    <dbReference type="Ensembl" id="ENSEAST00005032841.2">
        <property type="protein sequence ID" value="ENSEASP00005030207.2"/>
        <property type="gene ID" value="ENSEASG00005020566.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106839170"/>
    <dbReference type="CTD" id="3574"/>
    <dbReference type="GeneTree" id="ENSGT00390000004451"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005139">
        <property type="term" value="F:interleukin-7 receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042100">
        <property type="term" value="P:B cell proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045453">
        <property type="term" value="P:bone resorption"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019725">
        <property type="term" value="P:cellular homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097191">
        <property type="term" value="P:extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:homeostasis of number of cells within a tissue"/>
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        <property type="term" value="P:immune response"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0038111">
        <property type="term" value="P:interleukin-7-mediated signaling pathway"/>
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    <dbReference type="GO" id="GO:2001240">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway in absence of ligand"/>
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    <dbReference type="GO" id="GO:0035265">
        <property type="term" value="P:organ growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045579">
        <property type="term" value="P:positive regulation of B cell differentiation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030890">
        <property type="term" value="P:positive regulation of B cell proliferation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032722">
        <property type="term" value="P:positive regulation of chemokine production"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0001961">
        <property type="term" value="P:positive regulation of cytokine-mediated signaling pathway"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of organ growth"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:positive regulation of T cell differentiation"/>
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        <property type="term" value="P:T cell lineage commitment"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="Interleukin-7"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="IL-7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018049">
        <property type="entry name" value="IL-7/IL-9_CS"/>
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    <dbReference type="InterPro" id="IPR038325">
        <property type="entry name" value="IL7_sf"/>
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        <property type="entry name" value="INTERLEUKIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="INTERLEUKIN-7"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF01415">
        <property type="entry name" value="IL7"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="IL-7"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="INTERLEUKIN7"/>
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00255">
        <property type="entry name" value="INTERLEUKIN_7_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0202">Cytokine</keyword>
    <keyword evidence="8 12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0339">Growth factor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040494449" description="Interleukin-7" evidence="13">
        <location>
            <begin position="26"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="27"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="79"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="12">
        <location>
            <begin position="92"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007621"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019460"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
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        <source>
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    <evidence type="ECO:0000256" key="13">
        <source>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030207.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="197" mass="22652" checksum="FD7FBBFC20BC3EB8" modified="2023-09-13" version="2">MFHVSFRYIFGIPPLILVLLPVASSDCDIEGKDGKEYQNVLMISIDELGEFIRTERNLDSSPGLNTRFTDSMIEIDSNCLNNESNFFKKHSCDDNKEPLFLNRAARKMRQFLKMNISEDFNLHLARVSQGTLKLSNCTTKGKGRKPPSLGEAQPTKNLEENKSLKEQKKQNDLCFLKILLQKIKTCWNKILRDAKEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MTW3</accession>
    <name>A0A8C4MTW3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="19">Sidekick cell adhesion molecule 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">SDK1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Synapse</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the sidekick family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005034326.2">
        <property type="protein sequence ID" value="ENSEASP00005031580.2"/>
        <property type="gene ID" value="ENSEASG00005021355.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157747"/>
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        <property type="component" value="Chromosome 14"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048148">
        <property type="term" value="P:behavioral response to cocaine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060998">
        <property type="term" value="P:regulation of dendritic spine development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00063">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="CDD" id="cd00096">
        <property type="entry name" value="Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000202">
        <property type="entry name" value="Sidekick cell adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000253">
        <property type="entry name" value="Sidekick cell adhesion molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000158">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000177">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000206">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000209">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000231">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000236">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000237">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000261">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000266">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000271">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000301">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000359">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000360">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000420">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000434">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000485">
        <property type="entry name" value="Sidekick cell adhesion molecule 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003961">
        <property type="entry name" value="FN3_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036116">
        <property type="entry name" value="FN3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013098">
        <property type="entry name" value="Ig_I-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003598">
        <property type="entry name" value="Ig_sub2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170">
        <property type="entry name" value="PROTEIN SIDEKICK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44170:SF49">
        <property type="entry name" value="PROTEIN SIDEKICK-1 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00041">
        <property type="entry name" value="fn3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07679">
        <property type="entry name" value="I-set"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13927">
        <property type="entry name" value="Ig_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00014">
        <property type="entry name" value="FNTYPEIII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00060">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SMART" id="SM00408">
        <property type="entry name" value="IGc2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49265">
        <property type="entry name" value="Fibronectin type III"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50853">
        <property type="entry name" value="FN3"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="9 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0770">Synapse</keyword>
    <keyword evidence="3 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="1910"/>
            <end position="1933"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="25"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="214"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="307"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="401"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="17">
        <location>
            <begin position="495"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="591"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="692"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="793"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="895"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="993"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1097"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1200"/>
            <end position="1297"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1301"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1400"/>
            <end position="1497"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1502"/>
            <end position="1615"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1620"/>
            <end position="1702"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1703"/>
            <end position="1801"/>
        </location>
    </feature>
    <feature type="domain" description="Fibronectin type-III" evidence="18">
        <location>
            <begin position="1804"/>
            <end position="1902"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1977"/>
            <end position="2000"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2018"/>
            <end position="2046"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="2026"/>
            <end position="2037"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005031580.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="2113" mass="232388" checksum="941B70CA27BDC106" modified="2023-09-13" version="2">MPFSYVLHSGFFSFFSEYISDDVVPYFKTEPGLPQIHLEGNRLVLTCLAEGSWPLEFKWMHNDSELTTYTSEYKYIIPSLQRLDAGFYRCVVRNRMGALLQRRSEVQVAYMGNFMDTDQRKTVSQGHAAILNLLPITSCPRPQVTWFREGHKIIPSNRIAITLENQLVILAAVASDSGPYYVQAVNERNGENKTSPLIYLSVARDVGTPETMAPVIVIPPGNRSVVAGSSEVTLECIANARPVEDLSVSWKRNGVRITSGLHSFGRHLTISNPTSSDTGAYVCEATLRGSALEPARAKAFLSIIEPPYFTAEPESRILVEVEETVDIVCQAMGVPLPILQWYKDAISISKLQNPRYKVLSSGGLRIQKLRPEDSGIFQCFATNEGGEIQTYTYLDVTNVAPAFTQLPVDTTVTDGMTAILKCEVSGAPKPAITWKRGNHILASGSVRIPRFMLLESGGLQITPVFIQDAGNYTCYAANTEGSLNASAALTVWNRTSIINPPEDRVVIKGTTATLDCGATHDPRISLRYVWKKDNVVITPSSSSRIVVEKDGSLLISQTWSGDIGDYTCEIISEGGNDSRMARLEVIELPHSPQNLLASLNSSHSHTVMLSWVRPFDGNSPVLYYIVELSENNSPWKVHLSNVGPEMTGVAVRGLTPARTYQFRVCAVNQVGKGQYSTETSRLMLPEEPPSAPPKNIVASGRTNQSIMVQWQPPPETEHNGVLRGYILRYRLAGLPGEYQQRNITSPEVNYCLVTELIIWTQYEIQVAAYNGAGLGVFSRAVTEYTLQGVPTAPPQNVQAEAVNSTTIQFLWNPPPQQFINGINQGYKLLAWPADVPEASTVVTIAPDFHGVHHGYITNLKKFTAYLTSVLCFTTPGDGPPSAPQLIWTHEDKPGAVGHLSFTEILDTSLKVSWQEPLEKNGIITGYQISWEVHGKSDSRLTHTLSSTTHEHKIEGLSSLTTYTIEVAAVTAAGAGLATSSTISSGVPPELPGAPSNLVISNISPRSATLQFRPGYDGKTSISRWIIEGQVGAIGDEEEWVSLYEEENEPDAQMLEIPNLTPYTHYRFRMRQVNIVGASPFSQSSRVIQTLQAPPDVAPTSLTVRTASETSLRLRWVPLPDSQYNGNPESVGYRIKYWRPDLQSPALTQVINDRLEREFTIEELEEWTEYELQMQAFNAIGAGPWSEVVRGRTRESVPSAAPENVSAEAVSSTQILLTWASVPEQDQNGLILGYKILFRAKDLDPEPRSHVVRGNHTQSALLAGLRKFVLYELQVLAFTRIGNGVPSSPLVLERTKDDAPGPPVRLVFPEVRLTSVRIVWQPPEEPNGIILGYQIAYRLASSSPNTFTTVEVGATVRQFTATELAPESAYIFRLSAKTRQGWGEPLEATVITTEKRERPAPPRELLVPQAEVTARSLRLQWVPGSDGASPIRYFTVQVRELPRGEWQTYSSSISHEATACEVERLRPFTSYKLRLKATNDIGDSDFSVETEAVTTLQDVPGEPPSFISVTPHTTSSVLIQWQPPRDESLNGLLQGYRIYYRELEYEAASATESKMLKTPSALRAELTAQSSFKTVNSLSQLLNEAKYRRYEVVMTAYNIIGESPASAPVEVFVGEAAPALAPQNVQVNPLTASQLEVTWDPPPPESQNGNIQGYKAMLSRMVWLFLPERTVKLHTKYLVSISAFNAAGDGPKSDPRQGHTHEAAPGTPSFLAFSEITSTTLNVSWGEPLAANGILQGYRVVYEPLAPVQGVSKVVTVDVKGNRQRWLKVRDLTKGVTYFFRVQARTITYGPELQANVTAGPAVGSPGAPRHISVTKAASELTLQWTEGDAGKAPTTGYVIEARPSDEGLWDMFVKDIPRSATSYTVSLDRLRQGVTYEFRVVAVNQVGYGEPSRPSTAVSAQVETPFYEEWWFLLVMALSSLIIILLVVFALVLHGQNKKYKNCTTGKSLSNMEESVTLDNGGFAALELNSRHLNVKNTFSKKNGTRSPPRPSPGGLHYSDEDICNKYNGAVLTESMNLKEKSVDASESEGTDSESEDAPPQHSFVNHYMSDPTYYNSWKRRAPGGRAAPHRYQAVAGAEAGPPLHAVVTTQSAVFAPAGPGARTPLTGFSSFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MWT4</accession>
    <name>A0A8C4MWT4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transforming growth factor-beta-induced protein ig-h3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TGFBI</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Plays a role in cell adhesion. May play a role in cell-collagen interactions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Binds to type I, II, and IV collagens.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014683912.1">
        <property type="nucleotide sequence ID" value="XM_014828426.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MWT4"/>
    <dbReference type="SMR" id="A0A8C4MWT4"/>
    <dbReference type="Ensembl" id="ENSEAST00005032760.2">
        <property type="protein sequence ID" value="ENSEASP00005030143.1"/>
        <property type="gene ID" value="ENSEASG00005020514.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822996"/>
    <dbReference type="KEGG" id="eai:106822996"/>
    <dbReference type="CTD" id="7045"/>
    <dbReference type="GeneTree" id="ENSGT00530000063860"/>
    <dbReference type="OMA" id="MYQNIDI"/>
    <dbReference type="OrthoDB" id="286301at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031012">
        <property type="term" value="C:extracellular matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005518">
        <property type="term" value="F:collagen binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050840">
        <property type="term" value="F:extracellular matrix binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030198">
        <property type="term" value="P:extracellular matrix organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000001">
        <property type="entry name" value="periostin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000002">
        <property type="entry name" value="periostin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000003">
        <property type="entry name" value="periostin isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000007">
        <property type="entry name" value="Transforming growth factor-beta-induced protein ig-h3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050904">
        <property type="entry name" value="Adhesion/Biosynth-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011489">
        <property type="entry name" value="EMI_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016666">
        <property type="entry name" value="TGFBI/POSTN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900">
        <property type="entry name" value="PERIOSTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900:SF82">
        <property type="entry name" value="TRANSFORMING GROWTH FACTOR-BETA-INDUCED PROTEIN IG-H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016553">
        <property type="entry name" value="BIGH3_OSF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51041">
        <property type="entry name" value="EMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="3" id="KW-0301">Gamma-carboxyglutamic acid</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 13" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034005246" description="Transforming growth factor-beta-induced protein ig-h3" evidence="13">
        <location>
            <begin position="24"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="domain" description="EMI" evidence="15">
        <location>
            <begin position="45"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="14">
        <location>
            <begin position="103"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="14">
        <location>
            <begin position="240"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="14">
        <location>
            <begin position="375"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="14">
        <location>
            <begin position="502"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="683" mass="74314" checksum="CB6BCC8501CE4D53" modified="2022-01-19" version="1">MALLVRLLPLALALALGPAASLAGPARSPYQLVLQHSRLRGRQHGPNVCAVQKLIGTNKKYFTNCKQWYQRKICGKSTVISYECCPGYEKVPGEKGCPAALPLSNLYETLGVVGSTTTQLYTDRTEKLRPEMEGPGSFTIFAPSNEAWASLPAEVLDSLVSNVNIELLNALRYHMVNRRVLTDELKHGMALTSMYQNSDIQIHHYPNGIVTVNCARLLKADHHATNGVVHLIDKVISTVTNNIQQIIEIEDTFETLRAAVAASGLNTLLEGDGQFTLLAPTNEAFEKIPAETLNRILGDPEALRDLLKNHILKSAMCAEAIVAGMSMETLEGTTLEVGCSGDMLTINGKPIISNKDVLATNGVIHFIDELLIPDSAKTLFELAADSDVSTAVDLFRQAGLGTHLSGSERLTLLAPLNSVFKDGTPRIDAQMKNLLLNHIIKDQLASKYLYHGQTLDTLGGKKLRVFVYRNSLCIENSCIAAHDKRGRYGTLFTMDRVLTPPVGTVMDVLKGDNRFSMLVAAIQSAGLTETLNREGVYTVFAPTDEAFQALPPGELNKLLGNAKELANILKYHVGDEILVSGGIGALVRLKSLQGDKLEVSSKNNVVNVNKEPVAEADIMATNGVVYAINTVLQPPANRPQERGDELADSALEIFKQASAFSRAAQSSVKLAPVYQRLLERMKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MX68</accession>
    <name>A0A8C4MX68_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Rab9 effector protein with kelch motifs</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RABEPK</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Rab9 effector required for endosome to trans-Golgi network (TGN) transport.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MX68"/>
    <dbReference type="Ensembl" id="ENSEAST00005032958.2">
        <property type="protein sequence ID" value="ENSEASP00005030314.2"/>
        <property type="gene ID" value="ENSEASG00005020648.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158763"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052124">
        <property type="entry name" value="Rab9_kelch_effector"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46647">
        <property type="entry name" value="RAB9 EFFECTOR PROTEIN WITH KELCH MOTIFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46647:SF1">
        <property type="entry name" value="RAB9 EFFECTOR PROTEIN WITH KELCH MOTIFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="433"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="441"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030314.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="491" mass="53127" checksum="EDD9DE03962FB2A1" modified="2023-09-13" version="2">MPNPWHFACKSLPSSSGLLTQPRGESPRDDPTCRFPGHAQPSRPPGAQRAAPSKAGPGDPKQGSGWPPRSSGVSGRGEGSSPAPIRGGAKARDWLKPPQDTMKQLPVLEPGDKPRKATWYTLTPRGDSPCARVGHSCSYLPPVGDAKRGKLFIVGGADPNRSFSDVHTMDLATHQWDLATSEGLLPRYEHASFVPSCTPHSIWVFGGADQSGNRNCLQVLNPETRTWTMPEMTSPPPSPRTFHTSAAAIGNQLYVFGGGERGAQPVQDVELHVFDANTLTWSQPETLGIPPSPRHGHVMVAAGTKLFIHGGLAGDKFYDDLHCIDISRSLTLKSGDILIWKKKEGDMKWQKLSPTGAAPTGCAAHSGVAVGKHLYIFGGMTPTGALDTMYQYDIEKQHWTLLKFDTFLPPGRLDHSMCIIPWPVMCTSEKEDSNSVPLNCDAEKGESTDKRVTQGGDSHEESQTDTLLCFVFGGMNTEGEIYNDCIVTVVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MY29</accession>
    <name>A0A8C4MY29_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Lipocalin/cytosolic fatty-acid binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">LOC123275594</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the calycin superfamily. Lipocalin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MY29"/>
    <dbReference type="Ensembl" id="ENSEAST00005036076.2">
        <property type="protein sequence ID" value="ENSEASP00005033101.2"/>
        <property type="gene ID" value="ENSEASG00005022642.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012130"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006953">
        <property type="term" value="P:acute-phase response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002682">
        <property type="term" value="P:regulation of immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd19451">
        <property type="entry name" value="lipocalin_AGP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.128.20:FF:000012">
        <property type="entry name" value="Alpha-1-acid glycoprotein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001500">
        <property type="entry name" value="A1A_glycop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012674">
        <property type="entry name" value="Calycin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000566">
        <property type="entry name" value="Lipocln_cytosolic_FA-bd_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11967">
        <property type="entry name" value="ALPHA-1-ACID GLYCOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11967:SF2">
        <property type="entry name" value="ALPHA-1-ACID GLYCOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00061">
        <property type="entry name" value="Lipocalin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00708">
        <property type="entry name" value="A1AGLPROTEIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50814">
        <property type="entry name" value="Lipocalins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0011">Acute phase</keyword>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040515726" description="Lipocalin/cytosolic fatty-acid binding domain-containing protein" evidence="10">
        <location>
            <begin position="19"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="Lipocalin/cytosolic fatty-acid binding" evidence="11">
        <location>
            <begin position="40"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005033101.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="201" mass="23167" checksum="05BD7FA132230BBB" modified="2023-09-13" version="2">MALPWVLTVLSLLPLLDAQSLGCAKFRALPITDATLDRLSGKWFYIASASRNAGYKHSAKTYQAAFFYFDPSHKEDTIQLRAYSTIGNQCVYKSALLNVHRKEGTLSIHENGREQFAYVWLTKDPRTFMFAYFLDDKQNMGLSFYADRPEVTPEQMSEFHEAIGCIDIDKSEIMYTDEKKDQCGPLEKEHEEERKKKQEEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MYV4</accession>
    <name>A0A8C4MYV4_EQUAS</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="1 2">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MYV4"/>
    <dbReference type="Ensembl" id="ENSEAST00005032533.2">
        <property type="protein sequence ID" value="ENSEASP00005029936.2"/>
        <property type="gene ID" value="ENSEASG00005020358.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163677"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33426:SF44">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33426">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005029936.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="219" mass="24540" checksum="65601661F3D8942D" modified="2023-09-13" version="2">MVTEMGPRDKRLPTLTALIRFLSSVNSLMTTEFGARDKRFSTFTALIRPFSCVNSLMITEGGINGKRFSTFTALIRPFSCVNSQMICEGATTGKRFPTFTALIRPFSCVNSLMITKGRTRSKRFPTFTTCVRPFSCMNSLMIIEGRTTGKRFPTFTAHIRIFFCVNSPVLNECAPIFKRFLTLTALITPFSHVNSLVLTKVNAGSKSFPTFSTFIRPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MZF2</accession>
    <name>A0A8C4MZF2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">STK39</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4MZF2"/>
    <dbReference type="Ensembl" id="ENSEAST00005036436.2">
        <property type="protein sequence ID" value="ENSEASP00005033420.2"/>
        <property type="gene ID" value="ENSEASG00005022885.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154621"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010820">
        <property type="term" value="P:positive regulation of T cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06610">
        <property type="entry name" value="STKc_OSR1_SPAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000043">
        <property type="entry name" value="serine/threonine-protein kinase OSR1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000068">
        <property type="entry name" value="STE20/SPS1-related proline-alanine-rich protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000562">
        <property type="entry name" value="STE20/SPS1-related proline-alanine-rich protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024678">
        <property type="entry name" value="Kinase_OSR1/WNK_CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF14">
        <property type="entry name" value="STE20_SPS1-RELATED PROLINE-ALANINE-RICH PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12202">
        <property type="entry name" value="OSR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="1"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="296"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="314"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005033420.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="478" mass="53127" checksum="33AE4C568CCDB8F2" modified="2023-09-13" version="2">MSNGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLLTRTPDIAQRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAFSQEKSRRVKEENPEIAVNASSIPEQIQSLSVQDSQGLPNASEDYREASCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGAEIPDEVKLIGFAQLSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MZG0</accession>
    <name>A0A8C4MZG0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Microtubule associated protein 1 light chain 3 beta</fullName>
        </recommendedName>
    </protein>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Lipid-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATG8 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MZG0"/>
    <dbReference type="Ensembl" id="ENSEAST00005032309.2">
        <property type="protein sequence ID" value="ENSEASP00005029727.2"/>
        <property type="gene ID" value="ENSEASG00005020206.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154158"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016236">
        <property type="term" value="P:macroautophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000149">
        <property type="entry name" value="microtubule-associated proteins 1A/1B light chain 3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0072">Autophagy</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6 9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="lipid moiety-binding region" description="Phosphatidylserine amidated glycine; alternate" evidence="9">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR604241-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005029727.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="16855" checksum="F71CBB6592AFFD66" modified="2023-09-13" version="2">MFLPPTPLLSEKTFKLRCTFEQRVEGVRLTREQHPTKIPLPVLDKTKFLIPDHVYRSELLKRIRRRLPLNANQVFFLVVNGHSLVSASTPISEGYESKKDGDGFLCMAYASQETFGMNLSLSGIAPQNAFAIIVGKKRGTWGCMRKLNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N5W3</accession>
    <name>A0A8C4N5W3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Kelch like family member 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">KLHL9</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates the ubiquitination of AURKB and controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL9, KLHL13 and RBX1. Interacts with AURKB.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070351614.1">
        <property type="nucleotide sequence ID" value="XM_070495513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4N5W3"/>
    <dbReference type="SMR" id="A0A8C4N5W3"/>
    <dbReference type="Ensembl" id="ENSEAST00005035751.2">
        <property type="protein sequence ID" value="ENSEASP00005032804.2"/>
        <property type="gene ID" value="ENSEASG00005022429.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106824729"/>
    <dbReference type="KEGG" id="eai:106824729"/>
    <dbReference type="CTD" id="55958"/>
    <dbReference type="GeneTree" id="ENSGT00940000154359"/>
    <dbReference type="OrthoDB" id="1925334at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
        <property type="term" value="P:regulation of cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd18449">
        <property type="entry name" value="BACK_KLHL9_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18239">
        <property type="entry name" value="BTB_POZ_KLHL9_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000002">
        <property type="entry name" value="Kelch-like family member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000001">
        <property type="entry name" value="Kelch-like family member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000011">
        <property type="entry name" value="Kelch-like family member 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF11">
        <property type="entry name" value="KELCH-LIKE PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="2" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="11">
        <location>
            <begin position="50"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="595"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00056604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005032804.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="617" mass="69355" checksum="109523BEADE80B7D" modified="2023-09-13" version="2">MKVSLGNGDMGVSAHLQPCKAGTTRFFTSNTHSSVVLQGFDQLRIEGLLCDVTLVPGDGDEIFPVHRAMMASASDYFKAMFTGGMKEQDLMCIKLHGVNKVGLKKIIDFIYTAKLSLNMDNLQDTLEAASFLQILPVLDFCKVFLISGVSLDNCVEVGRIANTYNLIEVDKYVNNFILKNFPALLSTGEFLKLPFERLAFVLSSNSLKHCTELELFKAACRWLRLEDPRMECAAKLMKNIRFPLMTPQDLINYVQTVDFMRTDNTCVNLLLEASNYQMMPYMQPVMQSDRTAIRSDSTHLVTLGGVLRQQLVVSKELRMYDERAQEWRSLAPMDAPRYQHGIAVIGNFLYVVGGQSNYDTKGKTAVDTVFRFDPRYNKWMQVASLNEKRTFFHLSALKGHLYAVGGRSAAGELATVECYNPRMNEWSYVAKMCEPHYGHAGTVYGGLMYISGGITHDTFQNELMCFDPDTDKWTQKAPMTTVRGLHCMCTVGDKLYVIGGNHFRGTSDYDDVLSCEYYSPTLDQWTPIAAMLRGQSDVGVAVFENKIYVVGGYSWNNRCMVEIVQKYDPEKDEWHKVFDLPESLGGIRACTLTVFPPEENPGSPSRESPLSAPSDHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PF57</accession>
    <name>A0A8C4PF57_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Unk like zinc finger</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">UNKL</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the unkempt family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PF57"/>
    <dbReference type="Ensembl" id="ENSEAST00005000457.2">
        <property type="protein sequence ID" value="ENSEASP00005000370.2"/>
        <property type="gene ID" value="ENSEASG00005000274.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158822"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040594">
        <property type="entry name" value="UNK_Znf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057295">
        <property type="entry name" value="UNK_Znf_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057296">
        <property type="entry name" value="UNK_Znf_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045234">
        <property type="entry name" value="Unkempt-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493:SF37">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE UNKL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14493">
        <property type="entry name" value="UNKEMPT FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13920">
        <property type="entry name" value="zf-C3HC4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23261">
        <property type="entry name" value="zf-CCCH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25427">
        <property type="entry name" value="zf-CCCH_UNK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23035">
        <property type="entry name" value="zf-CCCH_UNK-like_4th"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18384">
        <property type="entry name" value="zf_CCCH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00356">
        <property type="entry name" value="ZnF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90229">
        <property type="entry name" value="CCCH zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="75"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="115"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="243"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="C3H1-type" evidence="11">
        <location>
            <begin position="285"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="10">
        <location>
            <begin position="698"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="75"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="115"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="243"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="8">
        <location>
            <begin position="285"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="336"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="392"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="449"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="558"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="336"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="392"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="477"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="563"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000370.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="739" mass="80263" checksum="6335CABF58F1FE54" modified="2023-09-13" version="2">MPSVSKAAAAALSGSPPQTEKPTHYRYLKEFRTEQCALFVQHKCTQHRPFTCFHWHFLNQRRRRPLRRRDGTFNYSPDVYCSKYDESSGECPDGDECPYLHRTTGDTERKYHLRYYKTGTCIHETDARGHCVKNGPHCAFAHGPLDLRPPVCDIRELQAQEALQNGQLGAGDGVPDLQPGVLASQAMIEKILGEDPRWQDTHFVLGSYKTEQCPKPPRLCRQGYACPHFHNSRDRRRNPRRFQYRSTPCPSVKHGDEWGEPSRCESGDSCQYCHSRTEQQFHPEIYKSTKCNDMRQTGYCPRGPFCAFAHVETCCPPFLAESLGMANDWGCRDLTSASSSAANSGQPGNAKRRDSPAEGSQKASDHDSKQTHLAVLAVVHPLAPSVSSSVASSLASSAGSGGSSPTALPALSARAHPLDPTGSTVESVPGSALDLHLSDVSLASLDKELEEQDGGDPGPPGQRSPGGSAPVTIPGCLPRSPSLHSSSSLSTSPLSSLSQSLSGPLVSSAMTPPQQPPPLTSEPGTLGPSAYSSLGLNGVPGNIWDFVSGSFSPSPSPILNAGPASSSSTSPSSAELARVRRQLDEAKRKIRQWEESWQQVQQACDAWQREAKEAKERALAADSARQLALQKKEEVEAQFRRLQEELEGRGVSSTLPGLRSCGDIGAIPLPKLHSLQSQLRLDLEAVDGVIFQLRAQQCVVCGERARGAVLRPCQHRVLCEPCAAGAPECPYCEGQPLPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PFE9</accession>
    <name>A0A8C4PFE9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">LOC106831616</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
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                <person name="M."/>
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                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
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            <location evidence="2">Membrane</location>
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        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
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                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
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                <person name="J."/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="2">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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        <location>
            <begin position="982"/>
            <end position="1000"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1019"/>
            <end position="1035"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1415"/>
            <end position="1430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF15308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005004126.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1430" mass="157945" checksum="0FC1B01F5A29273C" modified="2023-09-13" version="2">MRVPEEALKHEKFTIQLQLSQKSSESELSKSACAKSIDAKAADTVTEVQHKATEALKSEERAMDTSAVPRGTPLYGQPSWWGDDEVHEQRALKANGKPEEKNHETGASGCSIDAKQVEEQSAAANEEVLFPFCREPSYFEIPTKEFQQPSQITESTIHEIPTKDTPSSHTTGAGHASFTIEFDDSTPGKVTIRDHVTKFTSDQRHKSKKSSPGTQDLPGIQTGMMAPENKVADWLAQNNPPQMIWERTEEDSKSIKSDVPVYLKRLKGNKHDDGTQSDSENAGAHRRCSKRAALEEHLRRHHSEQKKLQKAQAPEKQQEQAATSSAHHRGGHGVPHGKLLKQKSEEPSVSIPFLQTALLRSSGSLGHRPSQEMDKMLKNQATSAASEKDNDDDQSDKGTYTIELENPNSEEVEARKMIDKVFGVDDNQDYNRPVINEKHKDLIKEWALNSAAVVMEERKPVTIPGFHNTEEGTSSSGNKRWVSQWASLAANHTRHDQEERIMELSVPVPLENETDISESGISVRSTGSGTSLASQGERRRRTLPQLPNEEKSLESSRAKVLMQRSEIGEKQDTELQEKEAPTQVYQKDQQDADRALNQMSRAVNGETLKTSGENKTLLHLGIASAGKEKSETEKEASSVKQTSVKVQQQEQREQAQWTPTKLSSSKSVSGQIDRAREESFKQESQPQEKIPGHSASKAERVIQSEGKRRKAEEVLKSQTSKGGDKKESSKSLVRQGSFTIEKPSPNIPIELIPHINKQTSSTPASAFTSASRIRERSDSMDTDSSLDTTLILKDTEAVMAFLEAKLREDNKTDEGPDTPSYNRDNSISPESDVDTASTISLVTGETERKSTQKRRSFTSLYKDRCSIGSPSKDVTKSSSGTREKIEKKTKSRSTEVGSRGDGRKFVQSSGRMRQPSVDLTDDDQTSSVPHSAISDIMSSDQETYSCKSHGRTPLTSTEEHAHSKLEGSKVTKSKTSPVAPGSSSKSTTLPRPRPTRTSLLRRARLGEASDSELADADKASVASEVSTTSSTSKPPTGRRNISRIDLLAQPRRTRLGSLSARSDSEATISRSSASSRTAEAIIRSGARLVPSDKFSPRIRANSISRLSDSKVKSMASAHGSPSVNSRWRRFPTDYASTSEDEFGSNRNSPKHTRLRTSPALKTTRLQSSGSTMPTSSSFKHRIKEQEDYIRDWTAHREEIARISQDLALIAREINDVAGEIDSVTSSGTAPSTTVSTAATTPGSAIDTREELVDRVFDESLNFRKIPPLVHSKTPEGNNCRSGDPRPQPAEPPDHLTITRRRTWSRDEVMGDNLLLSSVFQFSKKIRQSIDKTAGKIRILFKDKDRNWDEIESKLRAESEVPIVKTSSMEISSILQELKRVEKQLQAINAMIDPDGTLEALNNMGFPSVILPSPPKQKSSPVNNHSSPAML</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PHE6</accession>
    <name>A0A8C4PHE6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PHE6"/>
    <dbReference type="Ensembl" id="ENSEAST00005006269.2">
        <property type="protein sequence ID" value="ENSEASP00005005739.2"/>
        <property type="gene ID" value="ENSEASG00005004242.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182755"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002568">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000100">
        <property type="entry name" value="Zinc finger 45-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000184">
        <property type="entry name" value="Zinc finger protein 333"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000773">
        <property type="entry name" value="Zinc finger protein 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001270">
        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001016">
        <property type="entry name" value="zinc finger protein 850-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002179">
        <property type="entry name" value="Zinc finger protein 961"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050752">
        <property type="entry name" value="C2H2-ZF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384">
        <property type="entry name" value="FINGER PUTATIVE TRANSCRIPTION FACTOR FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24384:SF247">
        <property type="entry name" value="ZINC FINGER PROTEIN 977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="103"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="159"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="187"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="271"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="299"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="327"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="355"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="383"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="411"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005739.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="430" mass="49430" checksum="D56971C16E836E9C" modified="2023-09-13" version="2">MVGRLCESEDGSQCGENFSLLPNLSLNKKTTVGAKPWGCSACGRVFTHHSSLKTHIRCHSERKTYDYQKYGENPYKCKECGKAFTYLTLLRVHERTHTGEKPYECKKCSKAFRRSSHLRLHERTHTGEKPYECKKCNKAFTRSCSLQRHERLHTGEKPYECKKCSKAFARSCHLRLHERTHTGEKPYECKKCSKAFTTSRYLGLHEKTHTGEKPYECKKCSTAFTCSSNLQNHERIHTGEKPYECKKCSKAFTCSSSLQVHERTHTGEKPYECKKCSKAFASSSSLRLHETTHTGEKPYECKKCSKAFTHSCSLQRHERLHTGEKPYECKKCSKAFASSSSLRLHERAHTGEKPYECKKCSKAFTCSSYLRLHERIHTGEKPYECKKCSKAFKYSSSLRVHARIHTGEKPFECKICGKAFISTSSLSKHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PIQ9</accession>
    <name>A0A8C4PIQ9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Quinone oxidoreductase-like protein 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Ferry endosomal RAB5 effector complex subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Quinone oxidoreductase homolog 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Zeta-crystallin homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">CRYZL1</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the FERRY complex (Five-subunit Endosomal Rab5 and RNA/ribosome intermediary). The FERRY complex directly interacts with mRNAs and RAB5A, and functions as a RAB5A effector involved in the localization and the distribution of specific mRNAs most likely by mediating their endosomal transport. The complex recruits mRNAs and ribosomes to early endosomes through direct mRNA-interaction.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Early endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PIQ9"/>
    <dbReference type="Ensembl" id="ENSEAST00005009377.2">
        <property type="protein sequence ID" value="ENSEASP00005008601.2"/>
        <property type="gene ID" value="ENSEASG00005006175.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013113"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05195">
        <property type="entry name" value="enoyl_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.180.10:FF:000060">
        <property type="entry name" value="Crystallin zeta like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.180.10">
        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042633">
        <property type="entry name" value="CRYZL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020843">
        <property type="entry name" value="ER"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44461">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44461:SF1">
        <property type="entry name" value="QUINONE OXIDOREDUCTASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00829">
        <property type="entry name" value="PKS_ER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enoyl reductase (ER)" evidence="12">
        <location>
            <begin position="130"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="38"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="84"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00073876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00078317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00080963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008601.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="469" mass="50718" checksum="61817E79623698D4" modified="2023-09-13" version="2">MPGDVVPSAVAMAFSTVSGSPGVTSGLTNGSSAWARGGGLSAKGLEGGGGEGAGRWGRSEAGEPGETRGVSWETRIWLKLERRPAKPSSLGADGGSHRTPASDPLGPRPRSAKAAEGRVTMKGLYFQQSSTNEEITFVFQERENLPATEDYYVKLQVKACALSQINTKLLAEMKMEKDFFPVGREVAGIVLDVGSKVSFFQPDDEVVGILPLDSEDPGLCEVVRVHEHYLVHKPEKVTWPEAAGTIRDGVRAYTALHYLSHLSPGKSVLIMDGASALGTIAIQLAHHRGARVISTACSLEDKQYLERLRPSLARVIDLSNGKAHVAESCLEETGGLGVDIVLDAGVRLYSKDDEPAVKLQPLPHKHDIITLLGVGGHWVTTEENLQLDPPDSHCLFLKGATVAFLNDEVWNLSNVQQGKYLCILKDVMEKLSAGVFRPQLDEPIPLYEAKVSMEVVQKNQGRKKQVVQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PJC6</accession>
    <name>A0A8C4PJC6_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Family with sequence similarity 168 member B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">FAM168B</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAM168 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PJC6"/>
    <dbReference type="Ensembl" id="ENSEAST00005010787.2">
        <property type="protein sequence ID" value="ENSEASP00005009935.2"/>
        <property type="gene ID" value="ENSEASG00005007041.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106828815"/>
    <dbReference type="KEGG" id="eai:106828815"/>
    <dbReference type="CTD" id="130074"/>
    <dbReference type="GeneTree" id="ENSGT00390000005140"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029247">
        <property type="entry name" value="FAM168A/MANI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31844">
        <property type="entry name" value="MYELIN-ASSOCIATED NEURITE-OUTGROWTH INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14944">
        <property type="entry name" value="TCRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009935.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="195" mass="20297" checksum="27B03ED5856B6BDD" modified="2023-09-13" version="2">MNPVYSPGSSGVPYANAKGIGYPAGFPMGYAAAAPAYSPSMYPGANPTFQTGYTPGTPYKVSCSPTSGAVPPYSSSPNPYQTAVYPVRSAYPQQSPYAQQGTYYTQPLYAAPPHVIHHTTVVQPNGMPATVYPAPIPPPRGNGVTMGMVAGTTMAMSAGTLLTAHSPTPVAPHPVTVPTYRAPGTPTYSYVPPQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PKJ0</accession>
    <name>A0A8C4PKJ0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein zer-1 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Zyg-11 homolog B-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ZER1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="6">Interacts with the ELOC-ELOB/Elongin BC complex. Part of an E3 ubiquitin ligase complex including ZER1, CUL2 and Elongin BC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the zyg-11 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014719015.1">
        <property type="nucleotide sequence ID" value="XM_014863529.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PKJ0"/>
    <dbReference type="Ensembl" id="ENSEAST00005012958.2">
        <property type="protein sequence ID" value="ENSEASP00005011924.1"/>
        <property type="gene ID" value="ENSEASG00005007785.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106845425"/>
    <dbReference type="KEGG" id="eai:106845425"/>
    <dbReference type="CTD" id="10444"/>
    <dbReference type="GeneTree" id="ENSGT00530000063187"/>
    <dbReference type="OrthoDB" id="5783533at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031462">
        <property type="term" value="C:Cul2-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000111">
        <property type="entry name" value="Protein zer-1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000085">
        <property type="entry name" value="protein zer-1 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000160">
        <property type="entry name" value="Protein zer-1 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000225">
        <property type="entry name" value="Armadillo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056845">
        <property type="entry name" value="LRR_Zer-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055142">
        <property type="entry name" value="ZER1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051341">
        <property type="entry name" value="Zyg-11_UBL_adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12904">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12904:SF23">
        <property type="entry name" value="PROTEIN ZER-1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25013">
        <property type="entry name" value="LRR_Zer-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22964">
        <property type="entry name" value="ZER1-like_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00185">
        <property type="entry name" value="ARM"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0007">Acetylation</keyword>
    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Zer-1-like leucine-rich repeats region" evidence="10">
        <location>
            <begin position="193"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Protein zer-1 homolog-like C-terminal" evidence="9">
        <location>
            <begin position="401"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00062951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00081214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF25013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="766" mass="87951" checksum="FFA9EB09800EFAA3" modified="2022-01-19" version="1">MASDTPESLMALCTDFCLHNLEGTLGYLLDKETLRLHPDVFLPSEICDRLVNEYVELVNAACNFEPHESFFSLFSDPRSTRLTRIHLREDLVQDQDLEAIRKQDLVELYLTNCEKLSAKSLQTLRSFSHTLVSLSLFGCANIFYEEENPGGCEDECLVNPTCQVLVKDFTFEGFSRLRFLNLGRMIDGVPVESLLRPLNSLAALDLSGIQTSDAAFLTQWKDSLVSLVLYNMDLSEDHIRVIVQLHKLRHLDISRDRLSSYYKFKLTRKVLSLFVQKLGNLMSLDISGHMILENCSISKMDEEAGQTSIEPSKSSIMPFRALKRPLQFLGLFETSLCRLTHIPAYKVSGDKNEEQVLNAIEAYTEHRPEITSRAINLLFDIARIERCNQLLRALKLVITALKCHKYDKNIQVTGSAALFYLTNSEYRSEQSVKLRRQVIQVVLNGMESYQEVTVQRNCCLTLCNFSIPEELEFQYRRVNELLLSILNPTRQDESIQRIAVHLCNALVCQVDNDHKEAVGKMGFVVTMLKLIQKKLLDKICDQVMEFSWSALWNITDETPDNCEMFLNFNGMKLFLDCLKEFPEKQELHRNMLGLLGNVAEVKELRPQLMTSQFISVFSNLLESKADGIEVSYNACGVLSHIMFDGPEAWGICEPQREEVEERMWAAIQSWDINSRRNINYRSFEPILRLLPQGISPVSQHWATWALYNLVSVYPDKYCPLLIKEGGMPLLRDMIKMATARQETKEMARKVIEHCSNFKEENMDTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PQQ8</accession>
    <name>A0A8C4PQQ8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Dymeclin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">DYM</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the dymeclin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PQQ8"/>
    <dbReference type="Ensembl" id="ENSEAST00005025682.2">
        <property type="protein sequence ID" value="ENSEASP00005023672.2"/>
        <property type="gene ID" value="ENSEASG00005015845.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000008772"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019142">
        <property type="entry name" value="Dymeclin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12895">
        <property type="entry name" value="DYMECLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12895:SF9">
        <property type="entry name" value="DYMECLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09742">
        <property type="entry name" value="Dymeclin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0519">Myristate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005023672.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="724" mass="82249" checksum="C5599AA035A6A358" modified="2023-09-13" version="2">MGSNSSRIGDLPKNEYLKKLSGTESVSENDPFWNQLLSFSFPAPTSSTELKLLEEATISVCRSLVENNPRTGNFGALIKVFLSRTKELKLSAECQNHIFIWQTHNALFIICCLLKVFICEMSEEELQLHFTYEDKSPGTYSSDSEDLLEELLCCLMQLITDIPLLDITYEISVEAVSTMIVFLSCQLFHKEVLRQSISHKYLMQGRCLPYTSKLVKTLLYNFIRQEKPPPPGAHVFPQQSEAGGLLYGLASGVATGLWTVFTLGGVGSKAAATPELSSPLANQSLLLLLVLANLTDAADAPNPYKQAIMSFKNTQDSSPFPSSVPHAFQINFNSLYTALCEQQTSDQATLLLYTLLHQNSNVRTYMLARTDMENLVLPILEILYHVEERNSHHVYMALIILLILTEDDGFNRSIHEVILKNITWYSERVLTEISLGSLLILVVIRTIQYNMTRTRDKYLHTNCLAALANMSAQFRSLHQYAAQRIISYTCRHLRRYVCVLDKLCFPHSHCSTLRQCFSTLSDNGEELLSLTCSHILRSYASSLFSLLSKKHNKVLEQATQSLRGSLSSNDAPLPDYAQDLNVIEEVIRMMLEIINSCLTNSLHHNPNLVYALLYKRDLFEQFRTHPSFQDIMQNIDLVITFFSSRLLQAGAELSVERVLEIIKQGVVALPKDRLKKFPELKFKYVEEEQPEEFFIPYVWSLVYNSAVGLYWNPQDIQLFTMDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PSV5</accession>
    <name>A0A8C4PSV5_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="18">Solute carrier family 24 member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">SLC24A2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>Ca(2+)(out) + K(+)(out) + 4 Na(+)(in) = Ca(2+)(in) + K(+)(in) + 4 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:69967"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC24A subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044612708.1">
        <property type="nucleotide sequence ID" value="XM_044756773.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PSV5"/>
    <dbReference type="SMR" id="A0A8C4PSV5"/>
    <dbReference type="Ensembl" id="ENSEAST00005030686.2">
        <property type="protein sequence ID" value="ENSEASP00005028255.2"/>
        <property type="gene ID" value="ENSEASG00005019206.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106834006"/>
    <dbReference type="KEGG" id="eai:106834006"/>
    <dbReference type="CTD" id="25769"/>
    <dbReference type="GeneTree" id="ENSGT01030000234532"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
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            <fullName evidence="6">Sphingomyelin phosphodiesterase 4</fullName>
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        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="term" value="F:sphingomyelin phosphodiesterase D activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:cellular response to tumor necrosis factor"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:ceramide biosynthetic process"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001701">
        <property type="term" value="P:in utero embryonic development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006685">
        <property type="term" value="P:sphingomyelin catabolic process"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="Sphingomy_SMPD4"/>
    </dbReference>
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        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12988:SF6">
        <property type="entry name" value="SPHINGOMYELIN PHOSPHODIESTERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14724">
        <property type="entry name" value="mit_SMPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="819"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030786.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="866" mass="97384" checksum="38D75AEF5263F6A2" modified="2023-09-13" version="2">MTTFGRSVTERQPLSVRQATLWTPHCFPETAVSTSTGAVMAFPHLQQPSFLLASLKADSVNKPFAQRCQDLVKVIEDFPAKELQTIFPWLVESIFGSLDGVLVGWNLRCLQGRVSPVEYSIAMEFLDPGGPMMKLVYKLQAEDYKFDFPVSYLPGPVKASIQERVLPDSPLYHNKVQFPPPGGLGLNLALSPFEYYLFFFALSLITQKPLPGALHIRTSDCAYFILVDRYLSWFLPTEGNVLPLLSSSPGAPSPSPAPRTPAMPFASYGLHHTSLLKRHISHQTSVNADPASHEIWRSETLLQVFVEMWLHHYSLEMYQKMQSPHAKLEVLHYRLSVSSALHSPAQPSLQALHAYQEPFMPTEEHVLVVRLLLKHLHAFSHSLKAEQLSPSAHSHAASPLEEFKRAAVPRFVQQKLYLFLQHCFGHWPLDASFRAVLEMWLSYLQPWRYAPEKQAQSSDSQARCVSERWAPFVQENLLMYTKLFVGFLSRALRTDLVSPKNALMVFRVAKVFAQPNLAEMIQKGEQLFLEPELVIPHRQHRLLAAPPFTSSFLSSWPPAVTDNSFKVKSHVYGLEGQDCKYTPMFGPEVRTLVLRLAQLITQAKQTAKCLSDQCGESAAARPFLSWLGFCPTDTNGSYAANDLDELGQDSVRKTDEYLEKALEYLCQMFRLSEAQLAQLTLALGTTQDENGKKQLPDCIVGEDGLILTPLGRYQIINGLRKFDIEYQGDAELQPIRSYEIASLVRLLFRLSSAINCRFAGQMAALCSRADFLGSFCRYHLTEPGLADRHLLSPVQRGRTASRAQGPRLSLRFLGSYRTLLSLLLAFFVASLFCIGPLPCTLLLVLGYLLYAVAMTLLTERGKLHHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PVV7</accession>
    <name>A0A8C4PVV7_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Zinc finger protein 831</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ZNF831</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSEAST00005037031.2">
        <property type="protein sequence ID" value="ENSEASP00005033971.2"/>
        <property type="gene ID" value="ENSEASG00005023229.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161664"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47166">
        <property type="entry name" value="ZINC FINGER PROTEIN 831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47166:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 831"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="143"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="171"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="192"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="283"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="530"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="574"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="715"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="969"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1002"/>
            <end position="1085"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1101"/>
            <end position="1132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1210"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1318"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1367"/>
            <end position="1416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1518"/>
            <end position="1599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1663"/>
            <end position="1683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="194"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="212"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="316"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="338"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="608"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="732"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="797"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="823"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="839"/>
            <end position="848"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="860"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1033"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1273"/>
            <end position="1290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1340"/>
            <end position="1349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1381"/>
            <end position="1391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1545"/>
            <end position="1556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005033971.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1683" mass="177286" checksum="2A968698853265C4" modified="2023-09-13" version="2">MEVPELTCPASPARDQPAPAPGPPGAPGGQASPRLTLGPVILPPEQGLAPTVFLKALPIPLYHTVPPGGLQPRAPLVTGSLDGGNVPFILSPLLQPEGLGPTQMGKPVAPTLTVNIVGALPVLSPGLGPTLGSPGKVRNAGKYLCPHCGRDCLKPSVLEKHIRSHTGERPFPCATCGIAFKTQSNLYKHRRTQTHLNNSRLSSESDGGGGSHLEEGDKAGETSRADGRGDSWSQRVRDAAQSERPLSPGAQRAGHCPLPTTRLFPVAKNLDLKLEAIPCPGSTFTDREAPLDSTHTASAGLPVAGTQPRRKLLEQKSPTASRSCFVQQQQQQAMSAEKPWDPKASEGRLRKCESTDSGYLSRSDSVEQPLAPSSPLHSLSEHSAESEGEGAPGPGGARAEPGEQGPSLELEKKQLEERIARLISHNQAVVDDSQLDNVRPRKTVLSKQGSIDLPMPYTYKDSFHFDLRALEPGRRRQAALCSARSTFTPLDKTRPLFFHSVPTQLSTTVECVPVTRSNSLPFVEGTRMWQEPLDPQDTCPRRQKPLSPRPTPARLVGVPSSHPRALVRQAAVEDLPCPPTGDTPAPAEAPDGKRTAVGERGAGQARATSRKGSQRKLKMFSQEKWQVYGSETFKRIYQKMRASHHGSRKAKEETVGSGTGLDHPLQAEAAGCKGAAPAQDERTPVLGDISVGAKPGPWGSLPAPEGFLVTEPQKQRETVARAGGSDQPGVNRATSPPTLSCTEPPCLGSKSPQIPPKGRLELGCQLSPAPGPLKESDLQAPRLASPDPKLEGGTCNGRGTKTCQRAQTVLRQPSSNSGEPQALEDKLPSERKKLKVEELSCQEQSECSGAEGEAPGSPMQAASPSSQNQDSGPGNKLGGLHGSSDCMARGSAGQQGKPLETSSASSAASSVALWQVGPRAEGPSLCPAAAATMSPSQPAVQPRPPGALAAPADAAFPPKYLLRLPQGETHPSLLDARGPAQGQAPLCGRRCPEERAPFVESGLGALLPPSAVSGLAPGGEDSFEEDPCQSRLGDWRKGVQGDEKGDLDTSTPAAGESPGLAAGAPRETASFLPTPVCDSPRSATRDTRHLCMGSTLARARPSGGVLNPWAPNRELRAPPKSAPEDPPSGSLAGPSACCSLWPSSFLSAPMPSGWPELALCPHSETPGSSRARGPFPTLRAEPLLVWCCLSRSLPLPMEQKEKAASVYLALHSPGGTPRDKGPDAQPGRKAVSGGWPRTSPGEGGQAQTLKLSHPLAPGMASQDPVTEPEWKKGLRRRRVKMSRGSSKQKKLSLNSKRYKGNFSQSRVQLRAGRLRKPLWVPRKDCPPPPLKGLGPRRALQQASSETAGVNLQGEPSYAVSELSLRCGNGEEEEDDGGGQISGSFSPDISSRTAEETEETTVKAISPSAGEHGDCCPQNTAVGSGLSVQSDSRMAVANDSLLSCGKGLDMGLLELQLLPSQEQVSVDPKLCVFLDAQEPSSLESKGTSPRHDVATSVAATCTSVGARAGDTTLGIHSAEPQDYSQAAGETLTQSSPDRKARAEGISPSLLPGKSSSGQRISGSVPLGSTGKTHLEIPASGPDSTSSPQEEGRHKTSFPSRGQYECGEMVVSCPPIGKDSGKCQVSGLITLKDCVVPFNPGQPTEFPEAPLKTIRKRSLEGMRKQTRVEFSDTSSDDEDRLVIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I7L1</accession>
    <name>A0A9L0I7L1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">N-terminal EF-hand calcium-binding protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Neuronal calcium-binding protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">NECAB2</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">May act as a signaling scaffold protein that senses intracellular calcium. Can modulate ligand-induced internalization of ADORA2A and coupling efficiency of mGluR5/GRM5; for both receptors may regulate signaling activity such as promoting MAPK1/3 (ERK1/2) activation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts (calcium-dependent) with ADORA2A and GRM5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0I7L1"/>
    <dbReference type="Ensembl" id="ENSEAST00005079880.1">
        <property type="protein sequence ID" value="ENSEASP00005036434.1"/>
        <property type="gene ID" value="ENSEASG00005013703.2"/>
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    <dbReference type="GeneTree" id="ENSGT00950000183131"/>
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        <property type="component" value="Chromosome 28"/>
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        <property type="term" value="C:axon"/>
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        <property type="term" value="C:cytoplasm"/>
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    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0042984">
        <property type="term" value="P:regulation of amyloid precursor protein biosynthetic process"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.10.238.10:FF:000334">
        <property type="entry name" value="N-terminal EF-hand calcium binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.100:FF:000028">
        <property type="entry name" value="N-terminal EF-hand calcium-binding protein 2"/>
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        <property type="entry name" value="EF-hand"/>
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        <property type="entry name" value="ABM_dom"/>
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    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
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    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
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    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
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    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039862">
        <property type="entry name" value="NECAB1/2/3"/>
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    <dbReference type="PANTHER" id="PTHR12178">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12178:SF2">
        <property type="entry name" value="N-TERMINAL EF-HAND CALCIUM-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF03992">
        <property type="entry name" value="ABM"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF47473">
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        <property type="entry name" value="EF_HAND_2"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13" id="KW-0966">Cell projection</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="67"/>
            <end position="102"/>
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    <feature type="domain" description="EF-hand" evidence="19">
        <location>
            <begin position="103"/>
            <end position="136"/>
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    <feature type="domain" description="ABM" evidence="20">
        <location>
            <begin position="293"/>
            <end position="381"/>
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    <feature type="region of interest" description="Disordered" evidence="18">
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            <begin position="42"/>
            <end position="67"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
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            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023054"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00081394"/>
        </source>
    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51725"/>
        </source>
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    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005036434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="393" mass="43669" checksum="9E9EC4C8932260FB" modified="2023-09-13" version="1">MCERAARLCRAGAHRLLREPPPQGRALGGLLLRWVGARMGRAPGAAGPRRPAAAAADPGPGPASPRGGTAVILDIFRRADKNDDGKLSLEEFQLFFADGVLNEKELEDLFHTIDSDNTNHVDTKELCDYFVDHMGDYEDVLASLETLNHSVLKAMGYTKKVYEGGSNVDQFVTRFLLKETANQIQSLLSSVESAVEAIEEQTSQIRQNHSKASHGAVEPWYGSPTPPYAPNHKLMATEQGTSLPSVTADPKEEGLEAQISRLAELIGRLENKTLWFDLQQRLSDEEGTNMHLQLVRQEMAVCPEQLSEFLDSLRQYLRGTTGAKNCFHIAAVRLSDGLTFVVYEFWETEEEWKRHLQSPVCKAFRHAKVDTLSQPEALSRISVPATWCTVGRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I8H1</accession>
    <name>A0A9L0I8H1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106835794</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cell membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0I8H1"/>
    <dbReference type="Ensembl" id="ENSEAST00005004935.2">
        <property type="protein sequence ID" value="ENSEASP00005036847.1"/>
        <property type="gene ID" value="ENSEASG00005038317.1"/>
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        <property type="component" value="Chromosome 25"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15225">
        <property type="entry name" value="7tmA_OR10A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000385">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="59"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="97"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="131"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="196"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="236"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="271"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="40"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005036847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="310" mass="34267" checksum="1A9FB236403688F2" modified="2023-09-13" version="1">MKINQTILQEFILVGFSVYPHVQTFLFVVFFCLYLLTLTGNLAIIGLTCLDRALHNPMYLFLSALSFSETCYTLTIIPKMLADLLAKSRSISVIGCGLQMCFFLGLGGTNCIILTLMGYDRFLAICNPLRYPLLMTNMACGQLVASAWAGGFFISLIETALIFKGSFCIPNLVKHFFCHMRAVVRLNCLDSDLTEFIVTVISVSGLLGTFLLIILTYVFILSTVLRIPSAEGKQKAFSTCASHITVVIIHFGFASIVYLKPEASGGDDTLIAVPYTVITPFLSPLIFSLRNKDMKTAFGKVLGKARYLYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IA46</accession>
    <name>A0A9L0IA46_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL18m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L18, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MRPL18</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Together with thiosulfate sulfurtransferase (TST), acts as a mitochondrial import factor for the cytosolic 5S rRNA. The precursor form shows RNA chaperone activity; is able to fold the 5S rRNA into an import-competent conformation that is recognized by rhodanese (TST). Both the cytoplasmic and mitochondrial forms are able to bind to the helix IV-loop D in the gamma domain of the 5S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IA46"/>
    <dbReference type="Ensembl" id="ENSEAST00005051767.1">
        <property type="protein sequence ID" value="ENSEASP00005037642.1"/>
        <property type="gene ID" value="ENSEASG00005028667.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006394"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000005">
        <property type="entry name" value="39S ribosomal protein L18, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005484">
        <property type="entry name" value="Ribosomal_uL18_bac/plant/anim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L18, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12899:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL18M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00861">
        <property type="entry name" value="Ribosomal_L18p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="51"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00059887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00082661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005037642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="357" mass="38102" checksum="D68F82FF8C82F4BF" modified="2023-09-13" version="1">KPGSHRPSRAGWWPTPPPAHSGLARPYRSGSGWPPRCCGPQTRTAAPPSSPQGTRRASLTPQATSGSVPRPGGGGSGGSGGSGGSAERARATPRGLLGTPLPAPRARRRAGGGVRRESGQEVSSRKRAGPALSPNKRADEEGAKGGERKSGSGAAAGRRHWAEASPQAWVARAPLRILVPSRKGCGVAALSTRPTPAVKPHRDPLDNEAVAPEFTNRNPRNLELLAVARKERGWGTVWPSREFWHRLRVIKTQHHIEALVEHRSGKVVVSASTREWAIKKHLYRTKNVVACESIGRVLAERCLEAGINFMVYQPTPWEAASDSLKRLKSAMTEGGVVLQEPRRIYEGNSSSAGNGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IBN5</accession>
    <name>A0A9L0IBN5_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Synaptophysin like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">SYPL2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the synaptophysin/synaptobrevin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014690739.1">
        <property type="nucleotide sequence ID" value="XM_014835253.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IBN5"/>
    <dbReference type="Ensembl" id="ENSEAST00005080063.1">
        <property type="protein sequence ID" value="ENSEASP00005037621.1"/>
        <property type="gene ID" value="ENSEASG00005002976.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106827411"/>
    <dbReference type="KEGG" id="eai:106827411"/>
    <dbReference type="CTD" id="284612"/>
    <dbReference type="GeneTree" id="ENSGT01030000234637"/>
    <dbReference type="OMA" id="VFYLRFH"/>
    <dbReference type="OrthoDB" id="10006326at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008253">
        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001285">
        <property type="entry name" value="Synaptophysin/porin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10306">
        <property type="entry name" value="SYNAPTOPHYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10306:SF8">
        <property type="entry name" value="SYNAPTOPHYSIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00220">
        <property type="entry name" value="SYNAPTOPHYSN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="117"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="2">Belongs to the splicing factor SR family.</text>
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    <dbReference type="KEGG" id="eai:106839968"/>
    <dbReference type="CTD" id="10772"/>
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        <property type="term" value="P:negative regulation of mRNA splicing, via spliceosome"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="RBD_domain_sf"/>
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        <property type="entry name" value="RBM"/>
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    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
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        <property type="entry name" value="TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
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    <keyword evidence="5 13" id="KW-0694">RNA-binding</keyword>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="262" mass="31271" checksum="204A80D379AEFAB4" modified="2023-09-13" version="1">MSRYLRPPNTSLFVRNVADDTRSEDLRREFGRYGPIVDVYVPLDFYTRRPRGFAYVQFEDVRDAEDALHNLDRKWICGRQIEIQFAQGDRKTPNQMKAKEGRNVYSSSRYDDYDRYRRSRSRSYERRRSRSRSFDYNYRRSYSPRNSRPTGRPRRSRSHSDNDRFKHRNRSFSRSKSNSRSRSKSQPKKEMKAKSRSRSASHTKARGTSKTDSKTHYKSGSRYEKESRKKEPPRSKSQSRSQSRSRSKSRSRSWTSPKSSGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IKZ7</accession>
    <name>A0A9L0IKZ7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">LOC106833396</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Cell membrane</location>
            <topology evidence="2 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070374832.1">
        <property type="nucleotide sequence ID" value="XM_070518731.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IKZ7"/>
    <dbReference type="Ensembl" id="ENSEAST00005073780.1">
        <property type="protein sequence ID" value="ENSEASP00005038455.1"/>
        <property type="gene ID" value="ENSEASG00005034910.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106833396"/>
    <dbReference type="GeneTree" id="ENSGT00940000163826"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050939">
        <property type="entry name" value="Olfactory_GPCR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24242:SF197">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8 14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 15" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 14" id="KW-0675">Receptor</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="13 14" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="61"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="92"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="197"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="273"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="16">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="292" mass="32749" checksum="0B37D29371AA175D" modified="2023-09-13" version="1">MNNSATRTVTEFVLLGFPGCQQLQSFLFSLFFGIYMFTMMGNGAIVCAVRLDQQLHTPMYLLLGNFAFLEIWYITSTVPSMLVNFLSVTKSISFVGCFLQLYFFTSLGTTEAYFLCIMAYDRYLAICHPLHYTTVMTQRLCYILMSLCWVFGFLSYSVSTVQISQLPFCGPNTIDHFVCDMDPLIALSCTPAPVTEIIFYVLSSLIIILTFLYILSSYSLLLIAVLKIPSAAGRQKAFSTCGSHLTVVCLFFGALMAMYVSPTTDNPAEMQKIVTLFYSVVTPFLNPLIYSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0ILA7</accession>
    <name>A0A9L0ILA7_EQUAS</name>
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        <recommendedName>
            <fullName evidence="5">Sulfotransferase</fullName>
            <ecNumber evidence="5">2.8.2.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">HS3ST4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the sulfotransferase 1 family.</text>
    </comment>
    <dbReference type="EC" id="2.8.2.-" evidence="5"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0ILA7"/>
    <dbReference type="Ensembl" id="ENSEAST00005075459.1">
        <property type="protein sequence ID" value="ENSEASP00005037853.1"/>
        <property type="gene ID" value="ENSEASG00005030950.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161466"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008467">
        <property type="term" value="F:[heparan sulfate]-glucosamine 3-sulfotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002590">
        <property type="entry name" value="Sulfotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037359">
        <property type="entry name" value="NST/OST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000863">
        <property type="entry name" value="Sulfotransferase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605:SF11">
        <property type="entry name" value="HEPARAN SULFATE GLUCOSAMINE 3-O-SULFOTRANSFERASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10605">
        <property type="entry name" value="HEPARAN SULFATE SULFOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00685">
        <property type="entry name" value="Sulfotransfer_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Sulfotransferase" evidence="7">
        <location>
            <begin position="197"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="67"/>
            <end position="139"/>
        </location>
    </feature>
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            <begin position="158"/>
            <end position="192"/>
        </location>
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    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="1"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="74"/>
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            <begin position="123"/>
            <end position="134"/>
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        <location>
            <begin position="206"/>
            <end position="210"/>
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        <ligand>
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            <position position="287"/>
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        <ligand>
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            <dbReference type="Pfam" id="PF00685"/>
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        <source>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
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            <begin position="48"/>
            <end position="65"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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            <begin position="72"/>
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            <begin position="150"/>
            <end position="324"/>
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    </evidence>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IR19</accession>
    <name>A0A9L0IR19_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL11 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IR19"/>
    <dbReference type="Ensembl" id="ENSEAST00005058891.1">
        <property type="protein sequence ID" value="ENSEASP00005039529.1"/>
        <property type="gene ID" value="ENSEASG00005024989.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006922"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="4">
        <location>
            <begin position="61"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="98" mass="11229" checksum="2DD39C729C7D5ABE" modified="2023-09-13" version="1">RSWCFVSPGHQDWSPRLASWFCLQKKVFDDITKATSDWKGLRITVKLTIQNQQVQIEAVFSASALIITALQEPPRDQKKQKNMKHSGNITFDEIVNIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0ITH6</accession>
    <name>A0A9L0ITH6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LOC106847618</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the HSF family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014722462.1">
        <property type="nucleotide sequence ID" value="XM_014866976.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0ITH6"/>
    <dbReference type="Ensembl" id="ENSEAST00005040736.1">
        <property type="protein sequence ID" value="ENSEASP00005044281.1"/>
        <property type="gene ID" value="ENSEASG00005026082.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106847618"/>
    <dbReference type="KEGG" id="eai:106847618"/>
    <dbReference type="GeneTree" id="ENSGT00940000161825"/>
    <dbReference type="OrthoDB" id="6418155at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000349">
        <property type="entry name" value="Heat shock transcription factor, Y-linked"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF140">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR, X-LINKED MEMBER 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="9">
        <location>
            <begin position="80"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="196"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="204"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="220"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005044281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="358" mass="40003" checksum="DE7E5CAA3D451F27" modified="2023-09-13" version="1">MASQSTDQTEQVKQAPSGDREPATEVPSNSSLDPNLDSREILETHSDQAMSQDPGPQDNPRPQDPNQGTVHMEENHHLLGLSFPRKLWMVAEDDAFTSVRWNDEGDTVIIEEDLFQREILHQRGPGRIFETDSLKSFIRQLNLYGFSKIRPNDPSLHSSFSGNKRIMMYRNSKFQKDKPLLIENIRRRGDLRIIARPGTSATTPKRKKQVAATRRSPRIRHNEPTKAADEMAQKETPKAQGTSGTWWFLCSDICSVRCALENGRRSEPGSPSGEGTSRNSMFVPLATAGREGAGEPPTSPHYPDHDSVMSLYNTCYSILLAALSVMSSNEAPDEDEEHKGSSDYKCVLCEHCKDNPGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IUR1</accession>
    <name>A0A9L0IUR1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">CD300 molecule like family member g</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">CD300LG</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_070339426.1">
        <property type="nucleotide sequence ID" value="XM_070483325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IUR1"/>
    <dbReference type="Ensembl" id="ENSEAST00005038444.1">
        <property type="protein sequence ID" value="ENSEASP00005044716.1"/>
        <property type="gene ID" value="ENSEASG00005036131.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106836439"/>
    <dbReference type="CTD" id="146894"/>
    <dbReference type="GeneTree" id="ENSGT00940000162429"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05716">
        <property type="entry name" value="IgV_pIgR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000370">
        <property type="entry name" value="CMRF35-like molecule 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050671">
        <property type="entry name" value="CD300_family_receptors"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860:SF62">
        <property type="entry name" value="CMRF35-LIKE MOLECULE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11860">
        <property type="entry name" value="POLYMERIC-IMMUNOGLOBULIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0732">Signal</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040350690" evidence="8">
        <location>
            <begin position="18"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="264"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="14"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="147"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="183"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="204"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="232"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005044716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="358" mass="38781" checksum="67231E39A99A20F9" modified="2023-09-13" version="1">MRPLVLLWGCLVLPGYGVLVGPKEISGFEGDTLSLQCTYGEELRKHKKYWCREGGLLISRCSGTIYAEEDGQERTAGRVSVWDSPKELTLTVTLRNLTLQDAGKYWCGVSRLGFDWSFLVSLRVLPGPCCLPSPTPSFQPLATRSLQPKAKTWQTQPPELTSPGLHPTVTTAKQKTGAEASPFTGTSASRHTQASLYAGPAPYTGTSPHAVTSPFTGTPPHEATSPHAGTSRPATQLDSTSAETSLVPSSRSSKSRVSIPMVRILAPVLVLLSLLLTAGLAALGRYLLRRRKEAQLATETQRMEKVHLPHSEVVARSGREDRSREVGKPLRVQGGVLCFGDLPQSEKDVLSICSPLRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IUW7</accession>
    <name>A0A9L0IUW7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106843497</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014716017.1">
        <property type="nucleotide sequence ID" value="XM_014860531.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IUW7"/>
    <dbReference type="Ensembl" id="ENSEAST00005070422.1">
        <property type="protein sequence ID" value="ENSEASP00005044674.1"/>
        <property type="gene ID" value="ENSEASG00005023782.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106843497"/>
    <dbReference type="KEGG" id="eai:106843497"/>
    <dbReference type="GeneTree" id="ENSGT00940000156465"/>
    <dbReference type="OrthoDB" id="5967130at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15915">
        <property type="entry name" value="7tmA_OR12D-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050516">
        <property type="entry name" value="Olfactory_GPCR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26452">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="20"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="204"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="237"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="39"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005044674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="308" mass="34875" checksum="23072C37D7098E21" modified="2023-09-13" version="1">MLNQTSVAEFLLLGLTDIQILQPFLFVIFLAIYFLIVAGNGAILMIVISDPKLHSPMYFFLGNLSCLDICYSTVTLPKMLENFLSTHKAISFLGCISQLHFFHFLGSTEAMLLAVMAFDRFVAICKPLHYPLIMNHQLCTQMAITIWTIGFSHALLHSIMTSRLNFCGSNHIHHFFCDVKPLLELACGNTELNQWLLNTVTGTIAMGPFFLTLISYFYIIIYLFVKTRCCSMLHKALSTCASHFMVVILLYAPVVFTYIRPASGSSMDQDWIMAIMYSVVTPVLNPLIYTLRNKDVKGALSRVIRRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IV56</accession>
    <name>A0A9L0IV56_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RH1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="protein sequence ID" value="ENSEASP00005044769.1"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:JUN kinase binding"/>
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        <property type="term" value="F:kinesin binding"/>
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        <property type="term" value="F:MAP-kinase scaffold activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:signaling receptor complex adaptor activity"/>
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        <property type="term" value="P:vesicle-mediated transport"/>
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        <property type="entry name" value="C-Jun-amino-terminal kinase-interacting protein 3 isoform X1"/>
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        <property type="entry name" value="JIP3/JIP4"/>
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        <property type="entry name" value="C-JUN-AMINO-TERMINAL KINASE-INTERACTING PROTEIN 3"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="12"/>
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        <location>
            <begin position="183"/>
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        <location>
            <begin position="66"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="184"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51776"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="283" mass="32613" checksum="BE8969E76BD08661" modified="2023-09-13" version="1">MMEIQMDEGGGVVVYQDDYCSGSVMSERVSGLAGSIYREFERLIHCYDEEVVKELMPLVVNVLENLDSVLSENQEHEVELELLREDNEQLLTQYEREKALRKQAEEKFIEFEDALEQEKKELQIQVEHYEFQTRQLELKAKNYADQISRLEERESEMKKEYNALHQRHTEMIQTYVEHIERSKMQQVGGNSQTESSLPGRSRKERPTSLNVFPLADSTVRAQMGGKLVPAGDHWHLSDLGQLQFSSSYQVLSPCCGVRVSFPSPHVHSGSPFSAYNPLQLPWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IZX4</accession>
    <name>A0A9L0IZX4_EQUAS</name>
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        <recommendedName>
            <fullName evidence="7">E2 NEDD8-conjugating enzyme</fullName>
            <ecNumber evidence="7">2.3.2.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">UBE2F</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
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                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
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                <person name="Jiang"/>
                <person name="Q."/>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. Together with the E3 ubiquitin ligase RNF7/RBX2, specifically neddylates cullin-5 (CUL5). Does not neddylate CUL1, CUL2, CUL3, CUL4A or CUL4B. Mediates neddylation of the CUL9-RBX1 complex.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine + [E2 NEDD8-conjugating enzyme]-L-cysteine = [E1 NEDD8-activating enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with UBA3 and RBX2. Interacts (N-terminally acetylated form) with (via DCUN1 domain) DCUN1D1, DCUN1D2, DCUN1D3, DCUN1D4 and DCUN1D5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.34" evidence="7"/>
    <dbReference type="RefSeq" id="XP_044607239.1">
        <property type="nucleotide sequence ID" value="XM_044751304.2"/>
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    <dbReference type="AlphaFoldDB" id="A0A9L0IZX4"/>
    <dbReference type="SMR" id="A0A9L0IZX4"/>
    <dbReference type="Ensembl" id="ENSEAST00005071595.1">
        <property type="protein sequence ID" value="ENSEASP00005045683.1"/>
        <property type="gene ID" value="ENSEASG00005007386.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106832191"/>
    <dbReference type="KEGG" id="eai:106832191"/>
    <dbReference type="CTD" id="140739"/>
    <dbReference type="GeneTree" id="ENSGT00940000154349"/>
    <dbReference type="OMA" id="VMQYAKR"/>
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        <property type="component" value="Chromosome 19"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061654">
        <property type="term" value="F:NEDD8 conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd23794">
        <property type="entry name" value="UBCc_UBE2F_UBE2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000033">
        <property type="entry name" value="NEDD8-conjugating enzyme UBE2F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
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    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
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    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="13">
        <location>
            <begin position="32"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="10">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00060254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00065994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005045683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="399" mass="42940" checksum="9D2E536A5FF1D41F" modified="2023-09-13" version="1">MFFRKEWNSTYTFGIRGNEPIYTLTQEDCINSCCSTKSISGDKVCNLMIFDTRKTARQPNCYLFYCPSEEACPLKPAKGLMTYRIIRDFPSLARTDLPSQELAQEGPLFRGRSSQAVTPTAPPVTGDSKPTGVAWREAFSQKLGSSDHSEKLLKMDQAGPLFPVYKEKGRSQSSRFTSEQQIAHVLPENVTAFLTTGAKASLHAISATPEPALLPAASVPVTPSVTSQPQEPTSARPVARLTSQPPTVLIPTILTRAVVTPQAGLTTMAAPMTVSQAPTALTGPRDTVPSREMSTPTLNTEAAHSPATPPLSHVGSSTTNATASQGNGKASPGGSSLSSVPRSLHGLAFEKWLLTGTLLFGVLFLAIGLVLLGRMLSESLRRKRYSRLDYLINGIYVDI</sequence>
</entry>
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        <submittedName>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J2R8"/>
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        <property type="protein sequence ID" value="ENSEASP00005043570.1"/>
        <property type="gene ID" value="ENSEASG00005020383.2"/>
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        <property type="component" value="Chromosome 26"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:zinc ion binding"/>
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    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.160.60:FF:000800">
        <property type="entry name" value="zinc finger protein 181 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zinc finger protein 3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Zinc finger protein 550"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="zinc finger protein 583 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zinc finger protein 879 isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
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        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050758">
        <property type="entry name" value="Znf_C2H2-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234:SF8">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23234">
        <property type="entry name" value="ZNF44 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="12">
        <location>
            <begin position="12"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
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            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="230"/>
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            <begin position="258"/>
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        <location>
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            <begin position="342"/>
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            <begin position="370"/>
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        <location>
            <begin position="398"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
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            <begin position="77"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
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            <begin position="175"/>
            <end position="195"/>
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        <location>
            <begin position="87"/>
            <end position="99"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="115"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
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            <begin position="142"/>
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        </location>
    </feature>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="421" mass="48382" checksum="DFDB12596BDDB6DA" modified="2023-09-13" version="1">MAPPLDPVQVLVTFKDVAVTFTQEEWGQLDLHQRTLYQEVMLETCRLLVSLGHPVPTPEFFPLLEHRQELWTVRTGLSRSPCPGDTTKLQTREPSTSQLVLPEGDLLQGSLTEGSSRDSRLGQARDEEGLLEMQKGQWRPATEPHKETHPGEISLEDSHLGTHDSLHSRVLQERVPQGDVLHERDPQGPEKGPTIHAGSNLYKCKQCGKGFNRKWYLVRHQRVHTGMKPYECNACGKAFSQSSTLIRHYLIHTGEKPYQCMECGKAFKRRSYLLQHRPIHTGERPYECSQCGKAFTHRSTFIRHSRTHTGEKPFECKDCEKSFSNRAHLIQHSIIHTGEKPYECSECRKAFRCSSELMQHQRVHTGEKPYECTQCGKAFHRSTYLLQHSVIHTGETPYKCLECGKAFKRRSHLLQHQRVHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J341</accession>
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        <recommendedName>
            <fullName evidence="7">Ran GTPase-activating protein 1</fullName>
        </recommendedName>
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        <name evidence="10" type="primary">RANGAP1</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
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                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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    <comment type="similarity">
        <text evidence="6">Belongs to the RNA1 family.</text>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="Ran GTPase activating protein 1"/>
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    </dbReference>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Leu-rich_rpt"/>
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        <property type="entry name" value="LRR_dom_sf"/>
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        <property type="entry name" value="Ran_GTPase_activating_1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
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        <property type="entry name" value="RanGAP1_C_sf"/>
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    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF12">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
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    <dbReference type="Pfam" id="PF07834">
        <property type="entry name" value="RanGAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69099">
        <property type="entry name" value="Ran-GTPase activating protein 1 (RanGAP1), C-terminal domain"/>
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        <property type="entry name" value="RNI-like"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
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            <begin position="463"/>
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        <location>
            <begin position="407"/>
            <end position="490"/>
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            <begin position="407"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00060740"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00074239"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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            <dbReference type="Pfam" id="PF07834"/>
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            <dbReference type="Ensembl" id="ENSEASP00005044437.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
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    <accession>A0A9L0J749</accession>
    <name>A0A9L0J749_EQUAS</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
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                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        </location>
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            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="169" mass="18227" checksum="7EDBA9DC75E733FF" modified="2023-09-13" version="1">MLKMSQPKNPHKPVLSPLPQHLPSAGLQGLTCRKTHQSSGHCHCLRQNKTGRGQSPQETRLKKDCEITAPSTTVTVTMKRPRPAMTPTVSEEPLHARALHVVPEALRRTGRLPLAPGDLSRRVRRGPGAQVLVQGDVIGAVVGVLTVPAEEAPVRPHVAAIHVLEGVRP</sequence>
</entry>
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            <fullName evidence="17">Pulmonary surfactant-associated protein A</fullName>
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        <name evidence="21" type="primary">LOC106839384</name>
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    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Oligomeric complex of 6 set of homotrimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular space</location>
            <location evidence="2">Extracellular matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Surface film</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the SFTPA family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JED9"/>
    <dbReference type="Ensembl" id="ENSEAST00005054479.1">
        <property type="protein sequence ID" value="ENSEASP00005047585.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000156653"/>
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        <property type="term" value="C:collagen trimer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:carbohydrate binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:metal ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:respiratory gaseous exchange by respiratory system"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="CLECT_collectin_like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Pulmonary surfactant-associated protein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
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        <property type="entry name" value="C-type_lectin-like/link_sf"/>
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        <property type="entry name" value="C-type_lectin_CS"/>
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    <dbReference type="InterPro" id="IPR051077">
        <property type="entry name" value="Ca-dependent_lectin"/>
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    <dbReference type="InterPro" id="IPR033990">
        <property type="entry name" value="Collectin_CTLD"/>
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        <property type="entry name" value="CTDL_fold"/>
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        <property type="entry name" value="PULMONARY SURFACTANT-ASSOCIATED PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24024:SF13">
        <property type="entry name" value="PULMONARY SURFACTANT-ASSOCIATED PROTEIN A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57944">
        <property type="entry name" value="Triple coiled coil domain of C-type lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0106">Calcium</keyword>
    <keyword evidence="11" id="KW-0176">Collagen</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="6" id="KW-0305">Gaseous exchange</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0430">Lectin</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 19" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0767">Surface film</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040471832" description="Pulmonary surfactant-associated protein A" evidence="19">
        <location>
            <begin position="21"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="20">
        <location>
            <begin position="148"/>
            <end position="247"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="34"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="84"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022713"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023119"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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            <dbReference type="ARBA" id="ARBA00041095"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50041"/>
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    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005047585.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="248" mass="26035" checksum="FEA090D21FF92674" modified="2023-09-13" version="1">MLLCSLTLTLILLAVSGTKCDVKEFCAACSGVPGIPGSPGLPGRDGRDGVKGDPGPPGPIGPPGGMPGSPGHDGLIGPPGPPGERGDKGEPGERGPPGPPAYPDEELQTTLHDIRHQILQLMGALSLQGSVLAVGEKVFSTNGQVVDFDAVRESCARAGGRIAVPKSLEENAAIASFVTKHNTYAYLGLEEGPTAGDFYYLDGAPVNYTNWYPGEPRGRGKEKCVEMYTDGQWNDRSCLQYRLAICEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JF42</accession>
    <name>A0A9L0JF42_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Translation initiation factor eIF2B subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">eIF2B GDP-GTP exchange factor subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">EIF2B1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the translation initiation factor 2B (eIF2B) complex which is a heterodecamer of two sets of five different subunits: alpha, beta, gamma, delta and epsilon. Subunits alpha, beta and delta comprise a regulatory subcomplex and subunits epsilon and gamma comprise a catalytic subcomplex. Within the complex, the hexameric regulatory complex resides at the center, with the two heterodimeric catalytic subcomplexes bound on opposite sides.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the eIF-2B alpha/beta/delta subunits family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014714297.1">
        <property type="nucleotide sequence ID" value="XM_014858811.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JF42"/>
    <dbReference type="Ensembl" id="ENSEAST00005065539.1">
        <property type="protein sequence ID" value="ENSEASP00005050953.1"/>
        <property type="gene ID" value="ENSEASG00005012766.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106842340"/>
    <dbReference type="KEGG" id="eai:106842340"/>
    <dbReference type="CTD" id="1967"/>
    <dbReference type="GeneTree" id="ENSGT00550000074853"/>
    <dbReference type="OMA" id="GDWESCK"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005851">
        <property type="term" value="C:eukaryotic translation initiation factor 2B complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002183">
        <property type="term" value="P:cytoplasmic translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014003">
        <property type="term" value="P:oligodendrocyte development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1070:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2B subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000001">
        <property type="entry name" value="Translation initiation factor eIF-2B subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1070">
        <property type="entry name" value="Translation initiation factor eIF-2B, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051501">
        <property type="entry name" value="eIF2B_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042528">
        <property type="entry name" value="elF-2B_alpha_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45860:SF1">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF-2B SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00046432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="305" mass="34046" checksum="E56961DB505BB977" modified="2023-09-13" version="1">MDDKELIEYFKSQMKEDPDMASAVAAIQTLLEYLKRDKGETIQGLRANFTRAIKTLCGVDSSVAVSSGGELFLRFISLTSLEYSDYSKCKEIMIERGELFLRRISLSRNKIADLCHTFIKDGARILTHAYSRVVLRVLEAAVRAKKRFSVYITESQPDLSGKKMAKALCHLNVPVTVVLDAAVGYIMEKVDLVIVGAEGVVENGGIINKIGTNQMAVCAKAQNKPFYVVAESFKFVRLFPLNQQDVPDEFKYKPDTLKSAQTGQDLKEEHPWVDYTSPSLITLLFTDLGVLTPSAVSDELIKLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JGP1</accession>
    <name>A0A9L0JGP1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zinc finger CCHC domain-containing protein 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ZCCHC9</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">May down-regulate transcription mediated by NF-kappa-B and the serum response element.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JGP1"/>
    <dbReference type="Ensembl" id="ENSEAST00005046904.1">
        <property type="protein sequence ID" value="ENSEASP00005052491.1"/>
        <property type="gene ID" value="ENSEASG00005004088.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183041"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000017">
        <property type="entry name" value="zinc finger CCHC domain-containing protein 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.60.10:FF:000091">
        <property type="entry name" value="Zinc finger CCHC-type-containing 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042246">
        <property type="entry name" value="ZCCHC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46242:SF1">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46242">
        <property type="entry name" value="ZINC FINGER CCHC DOMAIN-CONTAINING PROTEIN 9 ZCCHC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="12">
        <location>
            <begin position="130"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="12">
        <location>
            <begin position="185"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="12">
        <location>
            <begin position="213"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00071051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="271" mass="30386" checksum="C7451EC28125321D" modified="2023-09-13" version="1">MTRWARVTTTNNKRPLRATSWEDMKKGAFEGKSQNLPKSKQLEGHRLSLKSDAPQAKHKKNKKKKEYLNEDVNGFMEYLRQNSQMVHNGEVIAADSQEVREEIAVALKKDSRREGRRLKRQATKKNAMVCFHCRKPGHGVADCPAALENHDMGTGICYRCGSTEHEITKCKAKVDPALGEFPFAKCFVCGEMGHLSRSCPDNPKGLYADGGCCRLCGSVAHFQKDCPESQNSDRTVTVGRWAKGMSADHEEILDVPKPQKPKTKIPKVVNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JH07</accession>
    <name>A0A9L0JH07_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Adaptor related protein complex 1 subunit sigma 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">AP1S3</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <location evidence="3">Clathrin-coated pit</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044607401.1">
        <property type="nucleotide sequence ID" value="XM_044751466.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JH07"/>
    <dbReference type="Ensembl" id="ENSEAST00005066851.1">
        <property type="protein sequence ID" value="ENSEASP00005051628.1"/>
        <property type="gene ID" value="ENSEASG00005023947.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106832468"/>
    <dbReference type="KEGG" id="eai:106832468"/>
    <dbReference type="CTD" id="130340"/>
    <dbReference type="GeneTree" id="ENSGT00970000193372"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030121">
        <property type="term" value="C:AP-1 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005905">
        <property type="term" value="C:clathrin-coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14831">
        <property type="entry name" value="AP1_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000005">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044733">
        <property type="entry name" value="AP1_sigma"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000804">
        <property type="entry name" value="Clathrin_sm-chain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00989">
        <property type="entry name" value="CLAT_ADAPTOR_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0168">Coated pit</keyword>
    <keyword evidence="10" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
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    </evidence>
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        <recommendedName>
            <fullName evidence="13">Vomeronasal type-1 receptor</fullName>
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                <person name="Guo"/>
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                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
    </evidence>
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        <source>
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                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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                <consortium name="Ensembl"/>
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        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000001">
        <property type="entry name" value="F-box/WD repeat-containing protein 11 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000004">
        <property type="entry name" value="F-box/WD repeat-containing protein 11 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021977">
        <property type="entry name" value="Beta-TrCP_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050995">
        <property type="entry name" value="WD-F-box_domain-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14604:SF5">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14604">
        <property type="entry name" value="WD40 REPEAT PF20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12125">
        <property type="entry name" value="Beta-TrCP_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM01028">
        <property type="entry name" value="Beta-TrCP_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="1 5" id="KW-0853">WD repeat</keyword>
    <keyword evidence="2" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="domain" description="F-box" evidence="6">
        <location>
            <begin position="190"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="312"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="339"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="379"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="422"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="462"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="502"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="551"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005053316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="605" mass="68860" checksum="892D13A370FF6E07" modified="2023-09-13" version="1">MDPAEAVLQEKALKFMCSMPRSLWLGCSSLADSMPSLRCLYNPGTGALTAFQNSSEREDCNNGEPPRKIIPEKNSLRQTYNSCARLCLNQETVCLASTAMKTENCVAKTKLTNGTSSMIVPKQRKLSASYEKEKELCVKYFEQWSESDQVEFVEHLISQMCHYQHGHINSYLKPMLQRDFITALPARGLDHIAENILSYLDAKSLCAAELVCKEWYRVTSDGMLWKKLIERMVRTDSLWRGLAERRGWGHYLFKNKPPDGNAPPNSFYRALYPKIIQDIETIESNWRCGRHSLQRIHCRSETSKGVYCLQYDDQKIVSGLRDNTIKIWDKSTLECKRILTGHTGSVLCLQYDERVIITGSSDSTVRVWDVNTGEMLNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMASPTDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWNTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLVAALDPRAPAGTLCLRTLVEHSGRVFRLQFDEFQIVSSSHDDTILIWDFLNDPAAQAEPPHSPSRTYTYISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JLI2</accession>
    <name>A0A9L0JLI2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106822160</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JLI2"/>
    <dbReference type="Ensembl" id="ENSEAST00005060106.1">
        <property type="protein sequence ID" value="ENSEASP00005054074.1"/>
        <property type="gene ID" value="ENSEASG00005028804.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282524"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15225">
        <property type="entry name" value="7tmA_OR10A-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="123"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="191"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="238"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="32"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005054074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="305" mass="33842" checksum="5AE8683DF307AF2B" modified="2023-09-13" version="1">MEFVFLGYPSNPEVCILFFLGVNLAYTLIICGHVLILVAIQTEARLHTPMYYFLGSLSAVEICYTAVVVPHILANIVQPEKTITLLGCATQMIFFIGLGSADCFLLAAMAYDRYVAICHPLQYPLIMTLTLCIHLVVASVVIGLFLSLQLVAFIFSLPFCRAQSIEHFFCDVPPVMHLVCANSHIHEQSVLVAATLAVAAPFFLITTSYAFIMVAVLKIHSAAGRHQAFSTCSSHLTVVLLQYGCCAFMYLCPNSIYYPKQDQFISLVYTLGTPLLNPLIYTLRNSEIKGILGRCLTRNYLFQNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JSS4</accession>
    <name>A0A9L0JSS4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytosolic Fe-S cluster assembly factor NUBP2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Nucleotide-binding protein 2</fullName>
            <shortName evidence="12">NBP 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 14" type="primary">NUBP2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the cytosolic iron-sulfur (Fe/S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. The NUBP1-NUBP2 heterotetramer forms a Fe-S scaffold complex, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Negatively regulates cilium formation and structure.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 4 [4Fe-4S] clusters per heterotetramer. Contains two stable clusters in the N-termini of NUBP1 and two labile, bridging clusters between subunits of the NUBP1-NUBP2 heterotetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotetramer of 2 NUBP1 and 2 NUBP2 chains. Interacts with KIFC1. Interacts with NUBP1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cytoplasm</location>
            <location evidence="12">Cytoskeleton</location>
            <location evidence="12">Microtubule organizing center</location>
            <location evidence="12">Centrosome</location>
        </subcellularLocation>
        <text evidence="12">Enriched at the centrosomes during mitosis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the Mrp/NBP35 ATP-binding proteins family. NUBP2/CFD1 subfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="ENSEASP00005052205.1"/>
        <property type="gene ID" value="ENSEASG00005035230.1"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000796">
        <property type="entry name" value="Cytosolic Fe-S cluster assembly factor NUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03039">
        <property type="entry name" value="NUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028600">
        <property type="entry name" value="NUBP2/Cfd1_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF19">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 12" id="KW-0408">Iron</keyword>
    <keyword evidence="9 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="82"/>
            <end position="89"/>
        </location>
        <ligand>
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        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
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            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
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        </ligand>
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        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <submittedName>
            <fullName evidence="2">Junctional cadherin complex regulator</fullName>
        </submittedName>
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        <name evidence="2" type="primary">JHY</name>
    </gene>
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        <name type="scientific">Equus asinus</name>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        </citation>
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        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00179">
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00183">
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="10">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="8">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005054472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="147" mass="16810" checksum="93194DA66925325A" modified="2023-09-13" version="1">MALQRIHKELLNLTHDPPAQCSAGPVGDDLFQWKATIMGPEDNPYQGGVFFLKIQFPFDYHFRPPKIAFTTRIYHPNINENGSICLDILGSQWSPVLTISKVLLSICSMLCDPNPNDPLVPEIAKIYLKDRQKYDRTAQEWTQTYAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JXA0</accession>
    <name>A0A9L0JXA0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">ATPase family gene 2 protein homolog A</fullName>
            <ecNumber evidence="3">3.6.4.10</ecNumber>
            <ecNumber evidence="4">3.6.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="27">AFG2 AAA ATPase homolog A</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="28">Ribosome biogenesis protein SPATA5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="26">Spermatogenesis-associated factor protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="29">Spermatogenesis-associated protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="33" type="primary">AFG2A</name>
    </gene>
    <organism evidence="33 34">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="33 34" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="33" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="33" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">ATP-dependent chaperone part of the 55LCC heterohexameric ATPase complex which is chromatin-associated and promotes replisome proteostasis to maintain replication fork progression and genome stability. Required for replication fork progression, sister chromatid cohesion, and chromosome stability. The ATPase activity is specifically enhanced by replication fork DNA and is coupled to cysteine protease-dependent cleavage of replisome substrates in response to replication fork damage. Uses ATPase activity to process replisome substrates in S-phase, facilitating their proteolytic turnover from chromatin to ensure DNA replication and mitotic fidelity. Plays an essential role in the cytoplasmic maturation steps of pre-60S ribosomal particles by promoting the release of shuttling protein RSL24D1/RLP24 from the pre-ribosomal particles. May be involved in morphological and functional mitochondrial transformations during spermatogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.10"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="24">Part of the 55LCC heterohexameric ATPase complex composed at least of AIRIM, AFG2A, AFG2B and CINP. Associates with pre-60S ribosomal particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="23">Belongs to the AAA ATPase family. AFG2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.10" evidence="3"/>
    <dbReference type="EC" id="3.6.4.6" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0JXA0"/>
    <dbReference type="Ensembl" id="ENSEAST00005072161.1">
        <property type="protein sequence ID" value="ENSEASP00005057916.1"/>
        <property type="gene ID" value="ENSEASG00005018182.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157323"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19503">
        <property type="entry name" value="RecA-like_CDC48_NLV2_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19511">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000567">
        <property type="entry name" value="ATPase, AAA family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000068">
        <property type="entry name" value="spermatogenesis-associated protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000069">
        <property type="entry name" value="spermatogenesis-associated protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000015">
        <property type="entry name" value="spermatogenesis-associated protein 5 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF27">
        <property type="entry name" value="ATPASE FAMILY GENE 2 PROTEIN HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0143">Chaperone</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="19" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="12" id="KW-0221">Differentiation</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="17" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="34" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="16" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="32">
        <location>
            <begin position="5"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="31">
        <location>
            <begin position="344"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="31">
        <location>
            <begin position="618"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="30">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="30">
        <location>
            <begin position="170"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00050527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00053563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00061477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00065200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00073856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00081938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00082434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00082694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00082953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="32">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005057916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="34">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="851" mass="93655" checksum="3B5D715C2992005B" modified="2023-09-13" version="1">MAASDLILCERRDDKVPKTFQNSLVQLGLNTMKSANICIGRPVLLTSLDGKQEVYTAWPVSGFPGGKVGLSEMAQKNIGVRIGDTIQVQPLLGAVLQAEEMDVALSDKNVDINEEQLTGCLLRKLDGKIVLPGNFLYCTFYGRPCKLQVLQVKGVDGTILRKPQSDSDTDTQGMTSKWSSTETSTLDISVQLSQLDLEEPQDPTSSSTPCKPVDDRMIKKAGDILLDVTQSPRDDGGLGLEEVTGLTCSFESAREGNEQLVNKERLLKSASLGAKCNTDTFYFISSTTRVSFTKIRTNSKEQDNKFKVTYDMIGGLNSQLKAIREIIELPLKQPELFKSYGIPPPRGVLLYGPPGTGKTMIARAVANEVGAYVSIINGPEIISKFYGETEARLRQIFAEATLRHPSIIFIDELDALCPKREGAQNEVEKRIVASLLTLMDGIGSEGSEGQVLVLGATNRPHALDAALRRPGRFDKEIEIGVPNAQDRLDILQKLVRRVPHLLTKAELLQLANSAHGYVGADLKALCNEAGLYALRRVLKKQPNLPDSKVAGLVKITLNDFLQGMNDIRPSAMREVAIDVPNVSWSDIGGVENIKLKLKQAVEWPLKHPESFIRMGIQPPKGVLLYGPPGCSKTMIAKALANESGLNFLAIKGPELMNKYVGESERAIREIFRKARAVAPSIIFFDELDALAIERGSSSGAGNVADRVLAQLLTEMDGIEQLKDVTILAATNRPDRIDKALMRPGRIDRIIYVPLPNAATRREIFNLQFHAMPISNEVDLDELILQTDSYSGAEIIAVCREAALLTLEEDIQARCIMRRHFTQALNTVTPRIPESLIRFYEDYQEKSGLHTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JXU8</accession>
    <name>A0A9L0JXU8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL15m</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">39S ribosomal protein L15, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC123283960</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JXU8"/>
    <dbReference type="Ensembl" id="ENSEAST00005060699.1">
        <property type="protein sequence ID" value="ENSEASP00005058101.1"/>
        <property type="gene ID" value="ENSEASG00005037034.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009040"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000006">
        <property type="entry name" value="39S ribosomal protein L15, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_uL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_uL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005749">
        <property type="entry name" value="Ribosomal_uL15_bac-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12934:SF11">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="10">
        <location>
            <begin position="69"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00035423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005058101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="249" mass="28053" checksum="62FD57F9D73D70ED" modified="2023-09-13" version="1">SRIRAPGNWKDDRGGGEEVEDVAEATDKGERQRGTRPRLGFEGGQTPFYIRIPKYGFNEGHSFRRQYQPLSLNRLQYLIDLGRVDPTQPIDLTQLVNGRGVTIQPYKRDYGVQLVEEGADTFKAKVNIEVQLASERAIAAIEESGGVVTTAFCDPRSLEILCKPVPFFLRGQPIPKRMLPPEALVPYYTDAKNRGDLADPAKFPEGRLELAQKYGYILPDVTKDELFKVLTTRKDPRQIFFGLAPGRGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JYT9</accession>
    <name>A0A9L0JYT9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Cyclin-dependent kinase inhibitor 3</fullName>
            <ecNumber evidence="4 11">3.1.3.16</ecNumber>
            <ecNumber evidence="3 11">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">CDKN3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">May play a role in cell cycle regulation. Dual specificity phosphatase active toward substrates containing either phosphotyrosine or phosphoserine residues.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with cyclin-dependent kinases such as CDK1, CDK2 and CDK3. Does not interact with CDK4. Interacts (via C-terminus) with phosphorylated CDK2 (via C-terminal helix). Interacts with MS4A3 (via C-terminus); the interaction enhances CDKN3 enzymatic activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
            <location evidence="1 11">Perinuclear region</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the protein-tyrosine phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="4 11"/>
    <dbReference type="EC" id="3.1.3.48" evidence="3 11"/>
    <dbReference type="RefSeq" id="XP_014720196.1">
        <property type="nucleotide sequence ID" value="XM_014864710.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JYT9"/>
    <dbReference type="SMR" id="A0A9L0JYT9"/>
    <dbReference type="Ensembl" id="ENSEAST00005070469.1">
        <property type="protein sequence ID" value="ENSEASP00005054275.1"/>
        <property type="gene ID" value="ENSEASG00005003194.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106846121"/>
    <dbReference type="KEGG" id="eai:106846121"/>
    <dbReference type="CTD" id="1033"/>
    <dbReference type="GeneTree" id="ENSGT00390000004717"/>
    <dbReference type="OMA" id="CRYKDIR"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14505">
        <property type="entry name" value="CDKN3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000046">
        <property type="entry name" value="Cyclin-dependent kinase inhibitor 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008425">
        <property type="entry name" value="CDK_inhib_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022778">
        <property type="entry name" value="CDKN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
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                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Microtubule inner protein component of sperm flagellar doublet microtubules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Flagellum axoneme</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014691748.1">
        <property type="nucleotide sequence ID" value="XM_014836262.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K1M4"/>
    <dbReference type="Ensembl" id="ENSEAST00005044047.1">
        <property type="protein sequence ID" value="ENSEASP00005059279.1"/>
        <property type="gene ID" value="ENSEASG00005016042.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106828024"/>
    <dbReference type="KEGG" id="eai:106828024"/>
    <dbReference type="CTD" id="93190"/>
    <dbReference type="GeneTree" id="ENSGT00390000014553"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005879">
        <property type="term" value="C:axonemal microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054709">
        <property type="entry name" value="CFAP107"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037662">
        <property type="entry name" value="CFAP68/107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31180:SF2">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31180">
        <property type="entry name" value="CILIA- AND FLAGELLA-ASSOCIATED PROTEIN 107-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22595">
        <property type="entry name" value="CFAP107"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0282">Flagellum</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005059279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="195" mass="23046" checksum="3A9177C5375C9AFC" modified="2023-09-13" version="1">MQLLTAVSPQSLSTPSWKIETKYSTRVLIGNWVEERRKFTKATEKTPQCIYRKEYVPFPGHRPDQISRWYGKRRIEGLPYKHLITHHQEPSHRHLISTYDDHYNRHNYNPGLPPRRTWNGHKLLWLPEKADFPLLAPPTNYGLHERLKQRWLKPVAGPREPVYTSSYPRPPLCALSQREHAIPVPPPRLHPVPHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K531</accession>
    <name>A0A9L0K531_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cx9C motif-containing protein 4</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Mature T-cell proliferation 1 neighbor protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CMC4</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CMC4 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K531"/>
    <dbReference type="Ensembl" id="ENSEAST00005039215.1">
        <property type="protein sequence ID" value="ENSEASP00005060459.1"/>
        <property type="gene ID" value="ENSEASG00005036735.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000012647"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1130:FF:000002">
        <property type="entry name" value="cx9C motif-containing protein 4 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1130">
        <property type="entry name" value="CytochromE C oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027179">
        <property type="entry name" value="CMC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15590">
        <property type="entry name" value="CX9C MOTIF-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15590:SF0">
        <property type="entry name" value="CX9C MOTIF-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08991">
        <property type="entry name" value="CMC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="27"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="7">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00080130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR627179-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005060459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="87" mass="10067" checksum="6064CACCA529072A" modified="2023-09-13" version="1">MHFIYFITLNPSINALSFLDMPQKDPCQKQACEIQKCLQANNYMESKCQAVIKELRKCCARYPKGRSLICSGFEKEDERLTLKSISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K631</accession>
    <name>A0A9L0K631_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106831379</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K631"/>
    <dbReference type="Ensembl" id="ENSEAST00005048265.1">
        <property type="protein sequence ID" value="ENSEASP00005060926.1"/>
        <property type="gene ID" value="ENSEASG00005030992.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157727"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000024">
        <property type="entry name" value="Anthrax toxin receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008400">
        <property type="entry name" value="Anthrax_toxin_rcpt_extracel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16059">
        <property type="entry name" value="ANTHRAX TOXIN RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16059:SF16">
        <property type="entry name" value="ANTHRAX TOXIN RECEPTOR-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05587">
        <property type="entry name" value="Anth_Ig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00092">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0732">Signal</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040485636" description="VWFA domain-containing protein" evidence="9">
        <location>
            <begin position="28"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="468"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="VWFA" evidence="10">
        <location>
            <begin position="63"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005060926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="627" mass="69238" checksum="29C633A6E9E215B1" modified="2023-09-13" version="1">MGSRGPRVPGPVLFLLLLLLPPPPLQSTESFPSRVPGNRDFRRKAGRRRRDVNEWKTCNGVFDVYFVLDASDIVKDNWEDIHKFVNETAKKYKNPKLRMSFITYSTEGHTLMNLTSDRNEIHDGLDRLKNVVPSGDRNLQEGFKKANEQIQQVHSKDQKAASLIIALTAGPLQQKTVQDAKNEAKKAQKMGTLIYGVGVKDYEASQLSNVAGSKNQIYEARKGFKSLRDIIGWLVVNSCAEVIPGGSYYVCVGESYEVTFHLSSSADINKDEVICRYKLGYSEPFYKNATSVQRGSVVCPGHVFKRGGQEVSIQSSQDNGTFYEKPMTLVSITCPDATTVPKETSIPTTVTQKTTVLTTVTQKKTTVTTTVTQEAPVPTVVTQKETSVPTTVTQKETPVPTTVTQKATPVPTTVTQKATPVPTVVTQKETPVPTVVTQKETPVPTTVTQKETSVPTVAPPGLSPDNPLFFPVLVPAVLIIPVLICCIWYCRRTVKEPPPVQKAEKEPETCPTVIVPWCGCQQDRIRRMEGKLDTLCNFVQSCNQVPLMWRDKGRCINFTLVKPPCVQMPCGSNICLRPSQESFPLNSCCSRGQPLRAICSRPASRMLPLIPPHAQALCRTTLSLPPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K685</accession>
    <name>A0A9L0K685_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Scavenger receptor class A member 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 14" type="primary">SCARA5</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Ferritin receptor that mediates non-transferrin-dependent delivery of iron. Mediates cellular uptake of ferritin-bound iron by stimulating ferritin endocytosis from the cell surface with consequent iron delivery within the cell. Delivery of iron to cells by ferritin is required for the development of specific cell types, suggesting the existence of cell type-specific mechanisms of iron traffic in organogenesis, which alternatively utilize transferrin or non-transferrin iron delivery pathways. Ferritin mediates iron uptake in capsule cells of the developing kidney. Binds preferrentially ferritin light chain (FTL) compared to heavy chain (FTH1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homotrimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SCARA5 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014698801.1">
        <property type="nucleotide sequence ID" value="XM_014843315.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K685"/>
    <dbReference type="Ensembl" id="ENSEAST00005070920.1">
        <property type="protein sequence ID" value="ENSEASP00005060976.1"/>
        <property type="gene ID" value="ENSEASG00005016320.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106832521"/>
    <dbReference type="KEGG" id="eai:106832521"/>
    <dbReference type="CTD" id="286133"/>
    <dbReference type="GeneTree" id="ENSGT00950000183074"/>
    <dbReference type="OMA" id="LCDEVST"/>
    <dbReference type="OrthoDB" id="536948at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070287">
        <property type="term" value="F:ferritin receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034605">
        <property type="term" value="P:cellular response to heat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070207">
        <property type="term" value="P:protein homotrimerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031638">
        <property type="term" value="P:zymogen activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.250.10:FF:000011">
        <property type="entry name" value="Scavenger receptor class A member 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.250.10">
        <property type="entry name" value="SRCR-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03070">
        <property type="entry name" value="SCARA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008160">
        <property type="entry name" value="Collagen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034726">
        <property type="entry name" value="SCARA5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001190">
        <property type="entry name" value="SRCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036772">
        <property type="entry name" value="SRCR-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071:SF24">
        <property type="entry name" value="DELETED IN MALIGNANT BRAIN TUMORS 1 PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48071">
        <property type="entry name" value="SRCR DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01391">
        <property type="entry name" value="Collagen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00530">
        <property type="entry name" value="SRCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00258">
        <property type="entry name" value="SPERACTRCPTR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00202">
        <property type="entry name" value="SR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56487">
        <property type="entry name" value="SRCR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00420">
        <property type="entry name" value="SRCR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50287">
        <property type="entry name" value="SRCR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0410">Iron transport</keyword>
    <keyword evidence="5 9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2 9 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="topological domain" description="Cytoplasmic" evidence="9">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="79"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="topological domain" description="Extracellular" evidence="9">
        <location>
            <begin position="100"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="domain" description="SRCR" evidence="13">
        <location>
            <begin position="410"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="319"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="331"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="382"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="435"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="448"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="10">
        <location>
            <begin position="479"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005060976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="512" mass="55485" checksum="D8A1B943EB7D17E4" modified="2023-09-13" version="1">MPETEATGSAASVEAQDRMENKAMYLHTVSDRDTSSIFEEPFDGRSLSKLNLCEDGPCHKRRTGGCCTQLGSLSALKHAVLGLYLLVFLILVGIFILAVSRPRSSPDDLKALTRNVNRLNESFRDLQLRLLQAPLQADLTEQVWKMQDALQNQSDSLLALAGAVQRLEGALWGLQAQAAQTEQAVALLRDSTGQQSDAAQLELYQLQVESNRSQLLLRRHAGLLDGLARRVGVLGEELADVGGALRGLNHSLSYDVALQGTRLRDLQVLVSNASEDTRRMRLVHMGMELQLKQELAVLNTVTEDLRLKDWEHSIALRNISLAKGPPGPKGDPGDKGKEGEPGTPGLPGLRGLPGERGIPGLPGPKGDDGKLGATGPMGMRGFKGDRGLKGEKGEKGDRAGDASAVEAVMIRLVNGSGPHEGRVEVYHDRRWGTVCDDGWDKKDGDVVCRMLGFRGAEEVSRTARFGQGTGRIWMDDVACKGTEDTIFRCSFSKWGVTNCGHAEDAGVTCNRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K7R8</accession>
    <name>A0A9L0K7R8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">UPAR/Ly6 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K7R8"/>
    <dbReference type="Ensembl" id="ENSEAST00005061397.1">
        <property type="protein sequence ID" value="ENSEASP00005061394.1"/>
        <property type="gene ID" value="ENSEASG00005030304.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159966"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007339">
        <property type="term" value="P:binding of sperm to zona pellucida"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23550">
        <property type="entry name" value="TFP_LU_ECD_Ly6K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018363">
        <property type="entry name" value="CD59_antigen_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016054">
        <property type="entry name" value="LY6_UPA_recep-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052874">
        <property type="entry name" value="Sperm-ZP_regulatory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035076">
        <property type="entry name" value="Toxin/TOLIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15049">
        <property type="entry name" value="GLYCOSYL-PHOSPHATIDYLINOSITOL-ANCHORED MOLECULE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15049:SF1">
        <property type="entry name" value="LYMPHOCYTE ANTIGEN 6K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00087">
        <property type="entry name" value="Toxin_TOLIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00134">
        <property type="entry name" value="LU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00983">
        <property type="entry name" value="LY6_UPAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="UPAR/Ly6" evidence="6">
        <location>
            <begin position="29"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="154" mass="17256" checksum="2EA2D3D95ECE6E20" modified="2023-09-13" version="1">MTLLLALLLAMGLPQVETNVTGSGKQDVLRCHVCERENNFNCAGPTNCSDRETYCSFAVVRIFPRFFYVSKQCAEYCSIKPAYLEKSFVIIKPMPFLYVMCCQTSLCNDAEPDIRDEEDNTKKKPKKASAVRSSRAGLLVFLTHASAFLGLRLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K852</accession>
    <name>A0A9L0K852_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kelch-like protein 15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">KLHL15</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Dimerization does not affect PPP2R5B-binding, but is required for its proteasomal degradation. Interacts with CUL3. Directly interacts with PPP2R5B; this interaction leads to PPP2R5B proteasomal degradation. Interacts with RBBP8/CtIP; this interaction leads to RBBP8 proteasomal degradation. Interacts with PACMP micropeptide; interaction prevents ubiquitination and degradation of RBBP8/CtIP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014723356.1">
        <property type="nucleotide sequence ID" value="XM_014867870.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044619193.1">
        <property type="nucleotide sequence ID" value="XM_044763258.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K852"/>
    <dbReference type="SMR" id="A0A9L0K852"/>
    <dbReference type="Ensembl" id="ENSEAST00005073041.1">
        <property type="protein sequence ID" value="ENSEASP00005058552.1"/>
        <property type="gene ID" value="ENSEASG00005017768.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106848135"/>
    <dbReference type="KEGG" id="eai:106848135"/>
    <dbReference type="CTD" id="80311"/>
    <dbReference type="GeneTree" id="ENSGT00940000159116"/>
    <dbReference type="OMA" id="PRHNSWL"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031463">
        <property type="term" value="C:Cul3-RING ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071630">
        <property type="term" value="P:nuclear protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18454">
        <property type="entry name" value="BACK_KLHL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18244">
        <property type="entry name" value="BTB_POZ_KLHL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000087">
        <property type="entry name" value="Kelch-like family member 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.420:FF:000009">
        <property type="entry name" value="Kelch-like protein 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.120.10.80:FF:000014">
        <property type="entry name" value="Kelch-like protein 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011705">
        <property type="entry name" value="BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017096">
        <property type="entry name" value="BTB-kelch_protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030597">
        <property type="entry name" value="BTB_POZ_KLHL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006652">
        <property type="entry name" value="Kelch_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047030">
        <property type="entry name" value="KLHL15_BACK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632:SF12">
        <property type="entry name" value="KELCH-LIKE PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45632">
        <property type="entry name" value="LD33804P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07707">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01344">
        <property type="entry name" value="Kelch_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037037">
        <property type="entry name" value="Kelch-like_protein_gigaxonin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00875">
        <property type="entry name" value="BACK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00612">
        <property type="entry name" value="Kelch"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="BTB" evidence="9">
        <location>
            <begin position="31"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00062127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005058552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="604" mass="69799" checksum="C91C5B740A5601A4" modified="2023-09-13" version="1">MAGDVEGFCSSIHDTSVSAGFRALYEEGLLLDVTLVIEDHQFQAHKALLATQSDYFRIMFTADMRERDQDKIHLKGLTATGFSHVLQFMYYGTIELSMNTVHEILQAAMYVQLIEVVKFCCSFLLAKICLENCAEIMRLLDDFGVNIEGVREKLDAFLLDNFVPLMSRPDFLSYLSFEKLMSYLDNDHLSRFPEIELYEAVQSWLRHDRRRWRHTDTIIQNIRFCLMTPSSVFEKVKTSEFYRYSRQLRYEVEQALNYFQNIHQQPLLDTKSSRIRSAKPQTTVFRGMIGHSMVNSKILLLKKPRVWWELEGPQVPLRPDCLAIVNNFVFLLGGEELGPDGEFHASSKVFRYDPRQNSWLRMADMSVPRSEFAVGVIGKFIYAVAGRTRDETFYSTERYDITNDKWEFVDPYPVNKYGHEGTVLNNKLFITGGITSSSTSKQVCVFDPSKEGTIEQRTRRTQVVTNCWENKSKMNYARCFHKMISYNGKLYVFGGVCVILRASFESQGCPSTEVYNPETDQWTILASMPIGRSGHGVTVLDKQIMVLGGLCYNGHYSDSILTFDPEENKWKEDEYPRMPCKLDGLQVCNLHFPEYILDEVRRCN</sequence>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Li"/>
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                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="J."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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            <authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <property type="component" value="Chromosome 9"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005059255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="101" mass="11657" checksum="691565AB872953C1" modified="2023-09-13" version="1">MISDDEHLFMCLLAIFISSFEKCLFMSSAHFLIVLFVFLLLSHVSSLYIMEINPLLDKWLVNIFSQLVSCFFVSILFSLALKKLFSLMKSHLFILSIVSLN</sequence>
</entry>
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    <accession>A0A9L0KEH0</accession>
    <name>A0A9L0KEH0_EQUAS</name>
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        <submittedName>
            <fullName evidence="2">Coiled-coil domain containing 15</fullName>
        </submittedName>
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    <gene>
        <name evidence="2" type="primary">CCDC15</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="protein sequence ID" value="ENSEASP00005060917.1"/>
        <property type="gene ID" value="ENSEASG00005002206.2"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:cilium assembly"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of centriole elongation"/>
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        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="CCDC15"/>
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        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 15"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 15"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="99"/>
            <end position="133"/>
        </location>
    </feature>
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        <location>
            <begin position="424"/>
            <end position="449"/>
        </location>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="437"/>
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        </location>
    </feature>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="Ensembl" id="ENSEASP00005060917.1"/>
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    </evidence>
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            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
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    <sequence length="798" mass="92358" checksum="44B5E2496243DB49" modified="2023-09-13" version="1">MPGSMAPLKKTGNTTRLPLALNPLKSKDVLAVLAERNLAIVPVGAWVEPASPHSSEIPASTSAYMIEEELKEELRKKQEALRRFQRQVKHRVNQQIRLRKKQQLQKSYEAAEKESSIAMQSSDPAHLTPKRTSVFPNNLNAAIGSARSLLSEMLGDGIEDRENQNELFLQQAQAEPESEESSSITVDKKGRENLFWEGWQDLLCEDKDKPFSRVQKVHFKNPLFAVMEEKELERLALQGLQDILPETHDYLPEAQDDLLETQGDLTGIQSVEPEAQSIEPDTQALELEGQAIEPKAQAIQPKAQDVKTETQGIMLEAQSTEPEDERIALKIQDFQPLNQAFLPKDQNILPTYQDQAFRPKDQHVLSKDQNILSKCQDQDFPPEDPDFLPRDQKAKLKEPASILIGGREREAFPLDVHQDLLHRHEDQASSRGKKVHFKEPYSDTTNEKGREDFSLAGYQYLPPKLQDQACIRDQNMFLEQPSSFMREGGDELPQEYHQFVKHKIQDQASSREQIEWNTNKCLKQPSSFEKWEIERGISPGVPSSLYFRQQPSVGTAERWREDLLLDGPQHLPPKHQREASRRRQVYDEYQSGLNTEFQTPLALQSGVDQEEDKKERQKQYLRYRRLFMDIEREQVKEQQRQKEHRKKIEKIKKKKEQQRYAEEQRILRMSFHKEPDSGEKMSEILAQLQLEEIKGAREKQQQREKEYQRYVEALRAQIQEKMQLYNVTLPPLCCCGPDFWDAHPDTCANNCIFYKNHRAYTRALHSVINSCDIPEGNSALRVAIHNFASAHRRTLKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KKM5</accession>
    <name>A0A9L0KKM5_EQUAS</name>
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        <recommendedName>
            <fullName evidence="14">Anamorsin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Cytokine-induced apoptosis inhibitor 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Fe-S cluster assembly protein DRE2 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14 17" type="primary">CIAPIN1</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
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                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13 14">Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis, facilitating the de novo assembly of a [4Fe-4S] cluster on the scaffold complex NUBP1-NUBP2. Electrons are transferred to CIAPIN1 from NADPH via the FAD- and FMN-containing protein NDOR1. NDOR1-CIAPIN1 are also required for the assembly of the diferric tyrosyl radical cofactor of ribonucleotide reductase (RNR), probably by providing electrons for reduction during radical cofactor maturation in the catalytic small subunit. Has anti-apoptotic effects in the cell. Involved in negative control of cell death upon cytokine withdrawal. Promotes development of hematopoietic cells.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Monomer. Interacts with NDOR1. Interacts with CHCHD4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="14">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="14">The C-terminal domain binds 2 Fe-S clusters but is otherwise mostly in an intrinsically disordered conformation.</text>
    </comment>
    <comment type="domain">
        <text evidence="14">The N-terminal domain has structural similarity with S-adenosyl-L-methionine-dependent methyltransferases, but does not bind S-adenosyl-L-methionine. It is required for correct assembly of the 2 Fe-S clusters.</text>
    </comment>
    <comment type="domain">
        <text evidence="14">The twin Cx2C motifs are involved in the recognition by the mitochondrial CHCHD4/MIA40-GFER/ERV1 disulfide relay system. The formation of 2 disulfide bonds in the Cx2C motifs through dithiol/disulfide exchange reactions effectively traps the protein in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the anamorsin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KKM5"/>
    <dbReference type="Ensembl" id="ENSEAST00005060299.1">
        <property type="protein sequence ID" value="ENSEASP00005062390.1"/>
        <property type="gene ID" value="ENSEASG00005005394.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011417"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030097">
        <property type="term" value="P:hemopoiesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000658">
        <property type="entry name" value="Anamorsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03115">
        <property type="entry name" value="Anamorsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007785">
        <property type="entry name" value="Anamorsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049011">
        <property type="entry name" value="Anamorsin_N_metazoan"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046408">
        <property type="entry name" value="CIAPIN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13273">
        <property type="entry name" value="ANAMORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13273:SF14">
        <property type="entry name" value="ANAMORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20922">
        <property type="entry name" value="Anamorsin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05093">
        <property type="entry name" value="CIAPIN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3 14" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 14" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 14" id="KW-0408">Iron</keyword>
    <keyword evidence="10 14" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Anamorsin N-terminal" evidence="16">
        <location>
            <begin position="64"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="Anamorsin C-terminal" evidence="15">
        <location>
            <begin position="325"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Fe-S binding site B" evidence="14">
        <location>
            <begin position="329"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cx2C motif 1" evidence="14">
        <location>
            <begin position="329"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cx2C motif 2" evidence="14">
        <location>
            <begin position="340"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="292"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="329"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00054715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF05093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005062390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="367" mass="38833" checksum="7E719ADE96FC44D7" modified="2023-09-13" version="1">MAEDVRGASVGHQVSAVLAAREPELPQRSPPGPLSSLNALISYSQPRPSVCSAAGMADFGISAGQFVAVIWDKSCPAEALKDLVDKLQALTGDEGHVSVENINQLLQSAHKESSFDIVLSGVIPGSSIVHSAEILAEVARILRPGGCLFLKEPVETAVVNNSKVRTASKLCSALTLSGLVEVKELQRESLSPEEMQSVQEHLGYHSDSLLSVQITGKKPNFEVGSSSQLKLSIAKKSSPSGKPAVDPAAAKLWTLSANDMEDDSVELIDSDDLLDPEDLKKPDPASLRAASCGEGKKRTACRNCTCGLAEELEKEKSRGQMSSQPKSACGNCYLGDAFRCASCPYLGMPAFKPGEKVLLSDSNLNDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KR24</accession>
    <name>A0A8C4KR24_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nucleotidyltransferase MB21D2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Mab-21 domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">MB21D2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Probable nucleotidyltransferase that catalyzes the formation of cyclic dinucleotide second messenger in response to some unknown stimulus.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the mab-21 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KR24"/>
    <dbReference type="Ensembl" id="ENSEAST00005000414.2">
        <property type="protein sequence ID" value="ENSEASP00005000331.2"/>
        <property type="gene ID" value="ENSEASG00005000343.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244976"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.40:FF:000005">
        <property type="entry name" value="MB21D2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.90:FF:000003">
        <property type="entry name" value="MB21D2 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046903">
        <property type="entry name" value="Mab-21-like_nuc_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046906">
        <property type="entry name" value="Mab-21_HhH/H2TH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024810">
        <property type="entry name" value="MAB21L/cGLR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656">
        <property type="entry name" value="CELL FATE DETERMINING PROTEIN MAB21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656:SF47">
        <property type="entry name" value="NUCLEOTIDYLTRANSFERASE MB21D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03281">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20266">
        <property type="entry name" value="Mab-21_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01265">
        <property type="entry name" value="Mab-21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Mab-21-like nucleotidyltransferase" evidence="9">
        <location>
            <begin position="96"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="Mab-21-like HhH/H2TH-like" evidence="10">
        <location>
            <begin position="285"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="391"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="391"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00054069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00073596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03281"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF20266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000331.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="451" mass="50960" checksum="CAB30CDE6D6CF425" modified="2023-09-13" version="2">MPSLPGKEETHPGEQAASPALLFFAANNFSGMVQKLDQKLPVANEYLLLSGGVREGVVDLDLDELNVYARGTDYDMDFTLLVPALKLHDRNQPVTLDMRHSALCHSWLSLRLFDEGTISKWKDCCTIVDHINGATNYFFSPTKVADWFYDSISIVLSEIQKKPQRGMPKVEKVEKNGTIISIILGVGSSRMLYDIVPVVSFKGWPAVAQSWLMENHFWDGKITEEEVISGFYLVPACSYKGKKDNEWRLSFARSEVQLKKCISSSLMQAYQACKAIIIKLLSRPKAISPYHLRSMMLWACDRLPANYLAQEDYAAHFLLGLIDDLQHCLVNKMCPNYFIPQCNMLEHLSEETVMLHARKLSSVRSDPAEHLRTAIEHVKAANRLTLELQRRGSTTSIPSPQSDGGDPNQPDDRLAKKLQQLVTENPGKSISVFINPDDVTRPHFRIDDKFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KX98</accession>
    <name>A0A8C4KX98_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Tetraspanin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">CD63</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Functions as a cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Interacts with TIMP1 and ITGB1 and recruits TIMP1 to ITGB1. Interacts with CD9. Identified in a complex with CD9 and ITGB3. Interacts with PMEL. Interacts with KDR/VEGFR2; identified in a complex with ITGB1 and KDR/VEGFR2 and is required to recruit KDR to ITGB1 complexes. Interacts with SYT7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cell surface</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Endosome</location>
            <location evidence="6">Multivesicular body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Lysosome membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Melanosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="24">Membrane</location>
            <topology evidence="24">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Secreted</location>
            <location evidence="5">Extracellular exosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8 24">Belongs to the tetraspanin (TM4SF) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044611996.1">
        <property type="nucleotide sequence ID" value="XM_044756061.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070350033.1">
        <property type="nucleotide sequence ID" value="XM_070493932.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070350034.1">
        <property type="nucleotide sequence ID" value="XM_070493933.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KX98"/>
    <dbReference type="Ensembl" id="ENSEAST00005003150.2">
        <property type="protein sequence ID" value="ENSEASP00005002876.1"/>
        <property type="gene ID" value="ENSEASG00005002208.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106841310"/>
    <dbReference type="KEGG" id="eai:106841310"/>
    <dbReference type="CTD" id="967"/>
    <dbReference type="GeneTree" id="ENSGT00940000156832"/>
    <dbReference type="OMA" id="RSWDIMQ"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031904">
        <property type="term" value="C:endosome lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005765">
        <property type="term" value="C:lysosomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042470">
        <property type="term" value="C:melanosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032585">
        <property type="term" value="C:multivesicular body membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097487">
        <property type="term" value="C:multivesicular body, internal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016477">
        <property type="term" value="P:cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035646">
        <property type="term" value="P:endosome to melanosome transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001046">
        <property type="term" value="P:positive regulation of integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002092">
        <property type="term" value="P:positive regulation of receptor internalization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901379">
        <property type="term" value="P:regulation of potassium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900746">
        <property type="term" value="P:regulation of vascular endothelial growth factor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03166">
        <property type="entry name" value="CD63_LEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1450.10:FF:000019">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1450.10">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042028">
        <property type="entry name" value="CD63_LEL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018499">
        <property type="entry name" value="Tetraspanin/Peripherin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000301">
        <property type="entry name" value="Tetraspanin_animals"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018503">
        <property type="entry name" value="Tetraspanin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008952">
        <property type="entry name" value="Tetraspanin_EC2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282:SF471">
        <property type="entry name" value="CD63 ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19282">
        <property type="entry name" value="TETRASPANIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00335">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002419">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00259">
        <property type="entry name" value="TMFOUR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48652">
        <property type="entry name" value="Tetraspanin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00421">
        <property type="entry name" value="TM4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="23" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0967">Endosome</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="20" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="19" id="KW-0458">Lysosome</keyword>
    <keyword evidence="16 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0564">Palmitate</keyword>
    <keyword evidence="14" id="KW-0653">Protein transport</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0964">Secreted</keyword>
    <keyword evidence="12 24" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15 24" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="51"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="24">
        <location>
            <begin position="203"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="145"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00006840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00043922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00046382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR002419-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU361218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005002876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="238" mass="25646" checksum="D1D4004D2871402A" modified="2022-01-19" version="1">MAVEGGMKCVKFMLYVLLLAFCACAVGLIAVGVATQVVLSQTIIPGATTGSLVPVVIIAVGAFLFLVAFVGCCGACKENYCLMITFAVFLSLITLVEVAAAIAGYVFRDRVMSEFSKDFKQQMQNYPKDNHTALILDRMQEKFHCCGATNYTDWEIIPLPVKGQVPDSCCVNVTQGCGTKFTEKDIYTKGCVEKIGGWLRKNVLVVAAAALGIAFVEVLGIVFACCLVKSIRSGYEVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KYM3</accession>
    <name>A0A8C4KYM3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ADP-ribosylation factor GTPase-activating protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Zinc finger protein 289</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ARFGAP2</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KYM3"/>
    <dbReference type="Ensembl" id="ENSEAST00005001174.2">
        <property type="protein sequence ID" value="ENSEASP00005001024.2"/>
        <property type="gene ID" value="ENSEASG00005000180.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155568"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048205">
        <property type="term" value="P:COPI coating of Golgi vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09029">
        <property type="entry name" value="ArfGap_ArfGap2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000004">
        <property type="entry name" value="Putative ADP-ribosylation factor GTPase-activating protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEIN 3, ISOFORM H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45686:SF10">
        <property type="entry name" value="ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12 17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Arf-GAP" evidence="19">
        <location>
            <begin position="11"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="138"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="383"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="269"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="397"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00043100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001024.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="573" mass="62134" checksum="2B5190EEF60276BB" modified="2023-09-13" version="2">MATEPSKTEIQTLFKRLRAIPTNKACFDCGAKNPSWASITYGVFLCIDCSGVHRSLGVHLSFIRSTELDSNWSWFQLRCMQVGGNANATAFFRQHGCTASDANTKYNSRAAQMYREKIRQLGSAALARHGTDLWIDNMTSASGHSPEKKDSDFFTEHTQPPAWNAPATDPAETQQPAPAESSGLAQPEHGPNTDLLGTSPKASLELKTSLIGKKKPAAAKKGLGAKKGLGAQKVSSQSFSEIERQAQVAEKLREQQVADAKKQAEESMVASMRLAYQELQIDRKKEEKKLQNLEGKKREQAERLGMGLVSRSSVSHSVLSEMQVIEQETPVTAKSSRSQLDLFDDVGTFASGPPKYKDNPFSLGESFGSRWDTDASWGMDRMEEKEPEVTISSIRPISERVTNRREAESRSSGLESSEARQKFAGAKAISSDMFFGREVDAEYEARSRLQQLSGSSAISSSDLFGDVDGAHGAGSVSLGNVLPTADIAQFKQGVKSVAGKMAVLANGVMNSLQVRLGLLALGWEGLRSSAWRLAFSTSSRHCCGEPLSLRACTFLEGQLSSSELGTRAGLSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KYR7</accession>
    <name>A0A8C4KYR7_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Synaptotagmin 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">SYT14</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">May be involved in the trafficking and exocytosis of secretory vesicles in non-neuronal tissues. Is Ca(2+)-independent.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Can also form heterodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the synaptotagmin family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KYR7"/>
    <dbReference type="Ensembl" id="ENSEAST00005003852.2">
        <property type="protein sequence ID" value="ENSEASP00005003518.2"/>
        <property type="gene ID" value="ENSEASG00005002709.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159420"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08389">
        <property type="entry name" value="C2A_Synaptotagmin-14_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08408">
        <property type="entry name" value="C2B_Synaptotagmin-14_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000153">
        <property type="entry name" value="Synaptotagmin 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000062">
        <property type="entry name" value="synaptotagmin-14 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043541">
        <property type="entry name" value="SYT14/14L/16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46129">
        <property type="entry name" value="SYNAPTOTAGMIN 14, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46129:SF3">
        <property type="entry name" value="SYNAPTOTAGMIN-14-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="7">
        <location>
            <begin position="249"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="7">
        <location>
            <begin position="404"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="215"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00056709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00063654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003518.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="563" mass="63230" checksum="41064FB0A465146C" modified="2023-09-13" version="2">MLTAVTFFSPVSPEAVGFLSAVGVFIILMLLLFLYINKKFCFENVGGFPDLGSGYSTRKNSQDKIYNSYMDKDEHCSSSESEDEALGKYHEALSRTHNSRLPLADSRQKNYTWETRQKYSPLSAEYDGYSSEGSIDEGNCIQRMRRTPPLDELQPPPYQDDSGSPHLSCTPSEMGDSKCEFSHCNSPRCSYNKCPSEGSTGHEIESFHNKGYEEEIPSDSTAVLSPEDMSARGSSSQLPKPFDPEPEAKYGTLDVTFDYDSQEQKLLVTVTAVTDIPTYNRTGGNSWQVHLVLLPIKKQRAKTSIQRGPCPVFTETFKFNHVESEMIGNYAVRFRLYGVHRMKKEKIVGEKIFYLTKLNLQGKMSLPVILEPSYNHSGCGSQMSVSEMSCSESTSSCQSLEHGSVPEILIGLLYHATTGRLSAEVIKGSHFKNLAANRPPNGLFCCLKHLIGGQVYIIRDTYVKLTLLNSMGQEMSKCKTSIRRGQPNPVYKETFVFQVALFQLSDVTLILSVYNKRSMKRKEMIGWISLGLNSSGEEELNHWTEMKESKGQQVCRWHALLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KZD4</accession>
    <name>A0A8C4KZD4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Thyroid hormone receptor alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Nuclear receptor subfamily 1 group A member 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="primary">THRA</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
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                <person name="F."/>
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                <person name="G."/>
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                <person name="Y."/>
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                <person name="Q."/>
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                <person name="Y."/>
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                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
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                <person name="G."/>
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                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
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                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
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                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
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                <person name="J."/>
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                <person name="A."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
            <location evidence="12">Nucleus</location>
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        <text evidence="1">Belongs to the nuclear hormone receptor family. NR1 subfamily.</text>
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        <property type="project" value="UniProtKB-KW"/>
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</entry>
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        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
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                <person name="Y."/>
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                <person name="Y."/>
                <person name="Min"/>
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                <person name="J."/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
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                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="J."/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
            <location evidence="1">Extracellular matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the TGF-beta family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014720199.1">
        <property type="nucleotide sequence ID" value="XM_014864713.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L0E8"/>
    <dbReference type="Ensembl" id="ENSEAST00005004758.2">
        <property type="protein sequence ID" value="ENSEASP00005004343.1"/>
        <property type="gene ID" value="ENSEASG00005003287.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106846123"/>
    <dbReference type="KEGG" id="eai:106846123"/>
    <dbReference type="CTD" id="652"/>
    <dbReference type="GeneTree" id="ENSGT00940000159502"/>
    <dbReference type="OMA" id="FQDVGWS"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008083">
        <property type="term" value="F:growth factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051216">
        <property type="term" value="P:cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071773">
        <property type="term" value="P:cellular response to BMP stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060441">
        <property type="term" value="P:epithelial tube branching involved in lung morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051241">
        <property type="term" value="P:negative regulation of multicellular organismal process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001503">
        <property type="term" value="P:ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030858">
        <property type="term" value="P:positive regulation of epithelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045778">
        <property type="term" value="P:positive regulation of ossification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090189">
        <property type="term" value="P:regulation of branching involved in ureteric bud morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043408">
        <property type="term" value="P:regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd19391">
        <property type="entry name" value="TGF_beta_BMP4_BMP2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.90.10:FF:000103">
        <property type="entry name" value="Bone morphogenetic protein 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.970:FF:000005">
        <property type="entry name" value="Bone morphogenetic protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047833">
        <property type="entry name" value="BMP4_TGF_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF165">
        <property type="entry name" value="BONE MORPHOGENETIC PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00250">
        <property type="entry name" value="TGF_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0891">Chondrogenesis</keyword>
    <keyword evidence="7" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="4" id="KW-0202">Cytokine</keyword>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-0221">Differentiation</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0272">Extracellular matrix</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11 16" id="KW-0339">Growth factor</keyword>
    <keyword evidence="10" id="KW-0892">Osteogenesis</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034326667" description="Bone morphogenetic protein 4" evidence="18">
        <location>
            <begin position="25"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="19">
        <location>
            <begin position="287"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="284"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00035683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005004343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="409" mass="46597" checksum="2D13A923661D09B6" modified="2022-01-19" version="1">MIPGNRMLMVVLLCQVLLGGASHASLIPETGKKKVAEIQGHAGGRRSGQSHELLRDFEATLLQMFGLRRRPQPSKSAVVPDYMRDLYRLQSGEEEEEEQIHSVGLEYPERPASRANTVRSFHHEEHLESIPGTSENSAFRFLFNLSSIPENEVISSAELRLFREQVDQGPDWEQGFHRINVYEVMKPPAEVVPGHLITRLLDTRLVHHNVTRWETFDVSPAVLRWTREKQPNYGLAIEVTHLHQTRTHQGQHVRISRSLPQGSGDWAQLRPLLVTFGHDGRGHALTRRRRAKRSPKHHPQRARKKTKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L3V3</accession>
    <name>A0A8C4L3V3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Angiomotin-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">AMOTL2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Podosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the angiomotin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014684207.1">
        <property type="nucleotide sequence ID" value="XM_014828721.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L3V3"/>
    <dbReference type="Ensembl" id="ENSEAST00005005756.2">
        <property type="protein sequence ID" value="ENSEASP00005005255.2"/>
        <property type="gene ID" value="ENSEASG00005003931.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106823188"/>
    <dbReference type="KEGG" id="eai:106823188"/>
    <dbReference type="CTD" id="51421"/>
    <dbReference type="GeneTree" id="ENSGT00940000156577"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005923">
        <property type="term" value="C:bicellular tight junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002102">
        <property type="term" value="C:podosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003365">
        <property type="term" value="P:establishment of cell polarity involved in ameboidal cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035329">
        <property type="term" value="P:hippo signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009114">
        <property type="entry name" value="Angiomotin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051747">
        <property type="entry name" value="Angiomotin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024646">
        <property type="entry name" value="Angiomotin_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14826">
        <property type="entry name" value="ANGIOMOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14826:SF3">
        <property type="entry name" value="ANGIOMOTIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12240">
        <property type="entry name" value="Angiomotin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01807">
        <property type="entry name" value="ANGIOMOTIN"/>
    </dbReference>
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        <text evidence="1">Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.</text>
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        <text evidence="5">Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD8, a base containing 6 ATPases and few additional components. Interacts with DDI2. Interacts with TASOR.</text>
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                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Dephosphorylates both phosphorylated Thr and Tyr residues in MAPK1, and dephosphorylation of phosphotyrosine is slightly faster than that of phosphothreonine. Can dephosphorylate MAPK1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:47005"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:10685"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with MAPK14; this interaction does not lead to catalytic activation of DUSP2 and dephosphrylation of MAPK14.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="10"/>
    <dbReference type="EC" id="3.1.3.48" evidence="10"/>
    <dbReference type="RefSeq" id="XP_044628829.1">
        <property type="nucleotide sequence ID" value="XM_044772894.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L5N3"/>
    <dbReference type="Ensembl" id="ENSEAST00005003339.2">
        <property type="protein sequence ID" value="ENSEASP00005003058.1"/>
        <property type="gene ID" value="ENSEASG00005002348.2"/>
    </dbReference>
    <dbReference type="GeneID" id="123286716"/>
    <dbReference type="KEGG" id="eai:123286716"/>
    <dbReference type="CTD" id="1844"/>
    <dbReference type="GeneTree" id="ENSGT00940000161605"/>
    <dbReference type="OMA" id="EARPVHW"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017017">
        <property type="term" value="F:MAP kinase tyrosine/serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001706">
        <property type="term" value="P:endoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043409">
        <property type="term" value="P:negative regulation of MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14641">
        <property type="entry name" value="DSP_DUSP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01446">
        <property type="entry name" value="DSP_MapKP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000034">
        <property type="entry name" value="Dual specificity phosphatase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000015">
        <property type="entry name" value="Dual specificity phosphatase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000340">
        <property type="entry name" value="Dual-sp_phosphatase_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008343">
        <property type="entry name" value="MKP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003595">
        <property type="entry name" value="Tyr_Pase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020422">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10159:SF109">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN PHOSPHATASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00782">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000939">
        <property type="entry name" value="MAPK_Ptase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01908">
        <property type="entry name" value="ADSPHPHTASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01764">
        <property type="entry name" value="MAPKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00195">
        <property type="entry name" value="DSPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00404">
        <property type="entry name" value="PTPc_motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50054">
        <property type="entry name" value="TYR_PHOSPHATASE_DUAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4 10" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="14">
        <location>
            <begin position="23"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine-protein phosphatase" evidence="12">
        <location>
            <begin position="172"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="13">
        <location>
            <begin position="237"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="11">
        <location>
            <position position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00057539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00065606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000939-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="314" mass="33980" checksum="8B28E6DBE3A5B8C6" modified="2022-01-19" version="1">MGLEAARELDCGALGALLREPREAERTLLLDCRPFLAFCRRHVRAARPVPWNALLRRRARGPPAAALACLLPDRALRARLARGELARVVVLDEGSASVAELAPDGPAHVLLAALLHETRAGPTAVCFLRGGFDGFQACCPDLCSESPVPALPPAAAENSRSDARAPFYDQGGPVEILPYLFLGSCSHSADLQGLQACGITAVLNVSASCPNHFEGLLHYKSIPVEDNQMVEISAWFQEAIGFIDSVKNSGGRVLVHCQAGISRSATICLAYLIQSRRVRLDEAFDFVKQRRGVISPNFSFMGQLLQFETQVLCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L6A9</accession>
    <name>A0A8C4L6A9_EQUAS</name>
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        <submittedName>
            <fullName evidence="1">Family with sequence similarity 200 member B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">FAM200B</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L6A9"/>
    <dbReference type="Ensembl" id="ENSEAST00005008150.2">
        <property type="protein sequence ID" value="ENSEASP00005007470.1"/>
        <property type="gene ID" value="ENSEASG00005005463.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913">
        <property type="entry name" value="EPM2A-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45913:SF19">
        <property type="entry name" value="LOW QUALITY PROTEIN: ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 5-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="657" mass="75761" checksum="5BBD3CB358A8D7CD" modified="2022-01-19" version="1">MDHFVFKRKRINEVKYAEACSSSTVGSDSVNSDSIEKNIDSNLQTSTLFEPHFKKKKVSARRYNEDYLKYGFIKCEKPFEKDRPQCVICNNILANESLKPSKLKRHLETQHAELIDKPLEYFQRKKKDVKLSTQFLSSTTVSEKALLSSYLVAYRVAKEKMAHTAAEKIILPACLDMVRTIFDDKSADKLKTIPSDNTISLRICTIAEHLETMLITRLQSGVDFAIQLDESTDIGSCMTLLVYVRYVWQDDFMEDFLCCLNLTSHLSGLNIFTELEKCIVGQYKLNWKNCKGITSDGTANITGKHSRVVKKLLEVTNNGAVWNHCFIHREALASREIPQNLMEVLKNAVKVVNFIKGSSLNSRLLEIFCSEIGTNHAHLLYHTKVRWLSHGKILSRVYELRNEIHIFLIEKKSHLASIFEDDIWVTKLAYLTDIFGILNELSLKLQGKSSDIFEHVERIQGFRKTLLLWEARLKSNHPSYYMFPRFLQHIEENIINENILKEIKLEILLHLTSLSQTFNHFFPEEKFETLRGNCWVKDPFAFRNPESIVELNLVPEEENELLQLSSSYTLKNDYETLSLSAFWIKIKEDFPLLSRKSVLLLLPFTTTSLCELGFSVLTKLKTKERNGLNGTADMRVALSSCVPDWNELINRQAHPSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L7C8</accession>
    <name>A0A8C4L7C8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">von Willebrand factor A domain containing 3A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">VWA3A</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
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                <person name="Min"/>
                <person name="J."/>
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                <person name="J."/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_070340873.1">
        <property type="nucleotide sequence ID" value="XM_070484772.1"/>
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        <property type="protein sequence ID" value="ENSEASP00005003783.2"/>
        <property type="gene ID" value="ENSEASG00005002837.2"/>
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    <dbReference type="GeneTree" id="ENSGT00940000159290"/>
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        <property type="component" value="Chromosome 14"/>
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        <property type="entry name" value="von Willebrand factor, type A domain"/>
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        <property type="entry name" value="VWF_A"/>
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    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46478">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46478:SF1">
        <property type="entry name" value="VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN 3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13768">
        <property type="entry name" value="VWA_3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="946"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="716"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="716"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003783.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1171" mass="132219" checksum="79BF477FEE00215D" modified="2023-09-13" version="2">MATQTSHDFHGQDNKFLENHCMRRNNGRDPKKQLNQKDINGLGQNPDDGLLVTHVNQTQDLLRLQGDETQPSAREDSEDWLSVHSLKFEKLTLADLIRQGTAVLEEGSNVMRKIHFSTEIIHQFESKLSEAVELYQQRIQWLTENSKKAFGLIKGARVGILIDVSAINSSPQTEEFQNDLVSLVDEQLSLKEKLYVLSFGTTTSALWPDPVEVKASTLQELKLWVKKLQPDVGSNLLQALKKIFAVKELNSLVTIMGSCPDQPSEILSDYIQQSVVGRGLITHIITYKCDHQVPPAVLRNLAEAVGGYYHCYSPETEICTSREVDELLAEIQKAQNLLGHVQALHHSTPWDELTCMMRELSTEIAKGPLTSLLPKPPKHEAPLTIEFPNLDKTSAEWLKINGLKAKKLSLYQVLAPNAFSPVEEFVPILQKTVSSTIHEKAMVQFEWHDGTVKNVHVDPPFLYEYQKQLSRAMRMYERRIEWLSLASRRIWGTVCEKRVVILLDISATNSMYIIHIQHSLRLLLEEQLSNKDHFNIIAFGSTIESWKPEMVAVSHDNLQSAWRWALTLQCRGSRNVLSALRKAVEVDFKDKDKHQSQGIYLFTGGVPDQDMPTLRAYVAEACGGCDLQLHICLFYVGEPQMDTTPPACYASRTDTAAAYRDVTRAAGGRFHWFGDTGIYESDDINAIMSEMEKALNYSQKCALLVSSLKNQSRKELESAALPKEKSKMLKQRSQPKKLCPSKPTAPSVARMSIKGGPDREKSPPLRALIWRPPSDQPGIPPAAAQPKREGTAERRRKTKSREAETSLLLFYTETGNNVGSVYKKYPQGRGVRRTSSSIELPRKNTVCSSQEWVANYGLKKLKMEISRYIGPKCSHQKSAQRSASAKYCSVFPSVEINGTVRHIQWTPRELEAYITHLEKVIRRYVQRLQWLLSGSRRLFGTILESKVCILLDTSGSMGPYLQQVKTELILLIWEQLRKHCDSFNLLSFAEGLQLWQDTLVETTDVACHEAMQWVTHLHAQGSTSVLEALLKAFSFPDVEGLYLLTDGKPDTSCSLILSEVQRLQENRDVKVHTISLSCSDRAAANFLRRLASLTGGRYHCPAGEDTLVRIHGLLTKGSVDERDPTLPLFEGDDLRRLAQEIAKARSFLWQAQSFRSQLQKKTNTEPKVALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LAF7</accession>
    <name>A0A8C4LAF7_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transmembrane protein 151A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TMEM151A</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM151 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LAF7"/>
    <dbReference type="Ensembl" id="ENSEAST00005007322.2">
        <property type="protein sequence ID" value="ENSEASP00005006698.1"/>
        <property type="gene ID" value="ENSEASG00005004946.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013762"/>
    <dbReference type="OMA" id="IHSCGAV"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026767">
        <property type="entry name" value="Tmem151"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31893">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 151 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31893:SF3">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 151A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14857">
        <property type="entry name" value="TMEM151"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="384"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="468" mass="51227" checksum="4ABF86F052C7C689" modified="2022-01-19" version="1">MPEDGGGDGGEVPALIPDGEPLREEQRPLKQSLGSSLCRESHWKCLLLTLLIHACGAVVAWCRLATVPRLVLGPEAALARGAGGPPPTYPASPCSDGYLYIPLAFVSLLYLLYLAECWHCHVRSCQAPRTDANTVLALIRRLQQAPPCVWWKATSYHYVRRTRQITRYRNGDAYTTTQVYHERADSRTARGEFDYSAHGVRDVSKELVGLADHAATRLRFTKCFSFGSAEAEASYLTQRARFFSANEGLDDYLEAREGMHLKDVDFRDSLMVFADPRSPPWYARAWVFWLVSAATLSWPLRVVAAYGTAHVHYQVEKLFGATSPPPGAVPSGPPLSRVATVDFTELEWHICSNRQLVPSYSEAVVMGAGSGAYLRGCQRCRRSVSSNSLPPARPSGPRLPFSRSRLSLGAGGRATPGVFRSLSGGPLGRRGEDTEPLESPPCYEDALYFPVLIVHGDSGCQGDGQGAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LAV4</accession>
    <name>A0A8C4LAV4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">T-cell surface glycoprotein CD3 delta chain</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">T-cell receptor T3 delta chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CD3D</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="15">The TCR-CD3 complex is composed of a CD3D/CD3E and a CD3G/CD3E heterodimers that preferentially associate with TCRalpha and TCRbeta, respectively, to form TCRalpha/CD3E/CD3G and TCRbeta/CD3G/CD3E trimers. In turn, the hexamer interacts with CD3Z homodimer to form the TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta. Interacts with coreceptors CD4 and CD8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014711077.1">
        <property type="nucleotide sequence ID" value="XM_014855591.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A8C4LAV4"/>
    <dbReference type="Ensembl" id="ENSEAST00005007528.2">
        <property type="protein sequence ID" value="ENSEASP00005006890.1"/>
        <property type="gene ID" value="ENSEASG00005024247.1"/>
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    <dbReference type="GeneID" id="106840277"/>
    <dbReference type="KEGG" id="eai:106840277"/>
    <dbReference type="CTD" id="915"/>
    <dbReference type="GeneTree" id="ENSGT00940000153312"/>
    <dbReference type="OMA" id="YQPLRDH"/>
    <dbReference type="OrthoDB" id="8941324at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
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    <dbReference type="GO" id="GO:0042105">
        <property type="term" value="C:alpha-beta T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045059">
        <property type="term" value="P:positive thymic T cell selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001361">
        <property type="entry name" value="T-cell surface glycoprotein CD3 delta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.770">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015484">
        <property type="entry name" value="CD3_esu/gsu/dsu"/>
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    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR032052">
        <property type="entry name" value="Ig_4"/>
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    <dbReference type="InterPro" id="IPR003110">
        <property type="entry name" value="Phos_immunorcpt_sig_ITAM"/>
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    <dbReference type="PANTHER" id="PTHR10570:SF5">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD3 DELTA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10570">
        <property type="entry name" value="T-CELL SURFACE GLYCOPROTEIN CD3 GAMMA CHAIN / DELTA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16680">
        <property type="entry name" value="Ig_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02189">
        <property type="entry name" value="ITAM"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00077">
        <property type="entry name" value="ITAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51055">
        <property type="entry name" value="ITAM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0391">Immunity</keyword>
    <keyword evidence="10 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-0675">Receptor</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 17" id="KW-0732">Signal</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034854695" description="T-cell surface glycoprotein CD3 delta chain" evidence="17">
        <location>
            <begin position="22"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="CD3 gamma/delta subunit Ig-like" evidence="18">
        <location>
            <begin position="31"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030628"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047083"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF16680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005006890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="165" mass="18391" checksum="D3514CF798BFC36F" modified="2022-01-19" version="1">MEHCRFLGSLILAALLSQVSLFKIDMDEIEERLILKCNSSIMQLEGTMGTPYKGGLDLGKRILDPRGTYMCGGAKENSSLQVYYRMCQNCVELDSATLAGIVITDIIATLLLALGVYCFAGHETGRLPRAADAQALLRNDQLYQPLRDRSDAQYSRLGENWPRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LB69</accession>
    <name>A0A8C4LB69_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Hypoxia-inducible factor 3-alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">HIF3-alpha-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">HIF3A</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044615015.1">
        <property type="nucleotide sequence ID" value="XM_044759080.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LB69"/>
    <dbReference type="Ensembl" id="ENSEAST00005005869.2">
        <property type="protein sequence ID" value="ENSEASP00005005366.2"/>
        <property type="gene ID" value="ENSEASG00005003993.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106845173"/>
    <dbReference type="KEGG" id="eai:106845173"/>
    <dbReference type="CTD" id="64344"/>
    <dbReference type="GeneTree" id="ENSGT00940000161745"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001525">
        <property type="term" value="P:angiogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071456">
        <property type="term" value="P:cellular response to hypoxia"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd19729">
        <property type="entry name" value="bHLH-PAS_HIF3a_PASD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000005">
        <property type="entry name" value="Hypoxia-inducible factor 1 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000042">
        <property type="entry name" value="hypoxia-inducible factor 3-alpha isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000076">
        <property type="entry name" value="hypoxia-inducible factor 3-alpha isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021537">
        <property type="entry name" value="HIF_alpha-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013767">
        <property type="entry name" value="PAS_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23043:SF18">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 3-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23171">
        <property type="entry name" value="bHLH_HIF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11413">
        <property type="entry name" value="HIF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00989">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14598">
        <property type="entry name" value="PAS_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50112">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0037">Angiogenesis</keyword>
    <keyword evidence="9" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="17" id="KW-0379">Hydroxylation</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0678">Repressor</keyword>
    <keyword evidence="13" id="KW-0346">Stress response</keyword>
    <keyword evidence="15" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="BHLH" evidence="22">
        <location>
            <begin position="33"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="21">
        <location>
            <begin position="109"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="PAS" evidence="21">
        <location>
            <begin position="265"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="372"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="444"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="533"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="20">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="568"/>
            <end position="581"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="621"/>
            <end position="632"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="648"/>
            <end position="664"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022657"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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            <dbReference type="ARBA" id="ARBA00022843"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023016"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023278"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00068506"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00077359"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50112"/>
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    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
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    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005366.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="687" mass="74216" checksum="7F4A78F5EEAC7D9F" modified="2023-09-13" version="2">MWQHPTCLSPPPLSLPGTPPVNAAHAGRSSTELRKEKSRDAARSRRSQETEVLYQLAHTLPFARGVSAHLDKASIMRLTISYLRMHRLCAAGEWNQVAAGGGPLDACYLKALEGFVMVLTAEGDMAYLSENVSKHLGLSQLELIGHSIFDFIHPCDQEELQDALTPRHSLSKKKPEAPTERCFSLRMKSTLTSRGRTLNLKAATWKVLHCSGHMRAYKPPAQTSPAGSPTLEPPLQCLVLICEAIPHPGSLEPPLGRGAFLSRHSLDMKFTYCDERIAEVAGYSPDDLIGCSAYEYIHALDSDAVGQSIHTLLSKGQAVTGQYRFLARSGGYLWTQTQATVVSGGRGPQSESIVCVHFLISRVEETEVVLSLEQTERHSRRPVQRGIPSQDGPNLGDSLDASGPRILAFLHPPSLSEAALAADPRRFCSPDLCRLLAPILDGTSAAATPSAPPAARRPQSPLPADLPDELLVDVENAHKVFASGKDLEAVETDLDIAQDVDALDLEMLAPYISMDDDFQLNSSEQLPRAYHRPLGAVPRPRARSFHGLSPPTPEPSLLPRWGSDPRLSCSSLSRGNPSASSPMAGARKRALAQSSEDEAEGVELLGVRPPKRSPSPDPENFLLPPLSLSFLLTGGPAPGSQQDPSTHLLELNEPLGLGPSLLSLYQDEDTAQPRSQFQPAAGLAQAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LBV0</accession>
    <name>A0A8C4LBV0_EQUAS</name>
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        <submittedName>
            <fullName evidence="10">Nuclear envelope integral membrane protein 1</fullName>
        </submittedName>
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    <gene>
        <name evidence="10" type="primary">NEMP1</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
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                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
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                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="Y."/>
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                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="W."/>
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                <person name="J."/>
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                <person name="G."/>
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                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
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                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
            <orientation evidence="1">Nucleoplasmic side</orientation>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the NEMP family.</text>
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        <property type="nucleotide sequence ID" value="XM_014843969.3"/>
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    <dbReference type="Ensembl" id="ENSEAST00005009641.2">
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        <property type="gene ID" value="ENSEASG00005006360.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 22"/>
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    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043131">
        <property type="term" value="P:erythrocyte enucleation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:nuclear membrane organization"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="InterPro" id="IPR019358">
        <property type="entry name" value="NEMP_fam"/>
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    <dbReference type="PANTHER" id="PTHR13598">
        <property type="entry name" value="AT07567P-RELATED"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR13598:SF2">
        <property type="entry name" value="NUCLEAR ENVELOPE INTEGRAL MEMBRANE PROTEIN 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF10225">
        <property type="entry name" value="NEMP"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="163"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="186"/>
            <end position="208"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="215"/>
            <end position="234"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="246"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="286"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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            <begin position="421"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
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            <begin position="421"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="433"/>
            <end position="444"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004575"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005748"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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    <sequence length="444" mass="50733" checksum="46EEC8E146008B77" modified="2023-09-13" version="2">MAGGMKVAVSPAVGAGPWGWGTGGGGAVRLLLVLSGCWVCGSAGIDVNVVMLQESRVDFVNTNQQFCYKNVLIPKWHDVWTRIQIRVNSSKLVRVTQVENEEKLKELEQFSIWNFFSSFLKEKLNDTYVNVGLYSTKTCLKVEIIEDDTTYSVIMTRRFDPKLFLVFLLGLTLFFCGDLLSRSQIFYYSTGMSVGIVASLLIIIFILSKFMPKKSPIYVILVGGWSFSLYLIQLVFKNLQEIWRCYWQYLLSYVLSVGFMSFAVCYKYGPLENERSINLLTWTLQLMGLCFMYSSIQIPHIALATVLIALCTKNLEYPIQWLYITYRKMCKATGKTVPPRLLTEEEYRIQGEVETRKALEELREYCNSPGCSPWKTVSRIQSPKRFADFVEGSFHLTPNEVSVHEQEYGLGSLIAQDEIYEETSSEEEDSDSQYPTVTQNNFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LHL5</accession>
    <name>A0A8C4LHL5_EQUAS</name>
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        <recommendedName>
            <fullName evidence="5">Centrosomal protein of 19 kDa</fullName>
        </recommendedName>
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        <name evidence="11" type="primary">CEP19</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
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            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
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                <person name="Y."/>
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                <person name="C."/>
                <person name="Wang"/>
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                <person name="Y."/>
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                <person name="Yu"/>
                <person name="J."/>
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                <person name="Yin"/>
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                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
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                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Cilium basal body</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
            <location evidence="1">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Spindle pole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the CEP19 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014723618.1">
        <property type="nucleotide sequence ID" value="XM_014868132.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014723619.1">
        <property type="nucleotide sequence ID" value="XM_014868133.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014723620.1">
        <property type="nucleotide sequence ID" value="XM_014868134.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014723621.1">
        <property type="nucleotide sequence ID" value="XM_014868135.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044627295.1">
        <property type="nucleotide sequence ID" value="XM_044771360.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044627296.1">
        <property type="nucleotide sequence ID" value="XM_044771361.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LHL5"/>
    <dbReference type="Ensembl" id="ENSEAST00005010241.2">
        <property type="protein sequence ID" value="ENSEASP00005009423.1"/>
        <property type="gene ID" value="ENSEASG00005006726.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106848323"/>
    <dbReference type="KEGG" id="eai:106848323"/>
    <dbReference type="CTD" id="84984"/>
    <dbReference type="GeneTree" id="ENSGT00390000016356"/>
    <dbReference type="OMA" id="AKKCGIQ"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034454">
        <property type="term" value="P:microtubule anchoring at centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097712">
        <property type="term" value="P:vesicle targeting, trans-Golgi to periciliary membrane compartment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029412">
        <property type="entry name" value="CEP19"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31539:SF1">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 19 KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31539">
        <property type="entry name" value="CENTROSOMAL PROTEIN OF 19K CEP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14933">
        <property type="entry name" value="CEP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0969">Cilium</keyword>
    <keyword evidence="7" id="KW-0970">Cilium biogenesis/degradation</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="163" mass="19282" checksum="3042B9093CD77B52" modified="2022-01-19" version="1">MTCTAKKCGIRFQPPAIILIYENEMKGKSRQRIMPVRNFSKFSDCSRAAEQLKNNPRHKSYLERVSLRQLEKLFTFLRGYLWGQSLAETMEQIQRETTIDPEEDLNKLDDKELAKRKSIMDELFEKNQKKKDDPNFVYDVEVEFPQDEQLQSCGWDTESADEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LIN1</accession>
    <name>A0A8C4LIN1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Small G protein signaling modulator 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SGSM1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RUTBC family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LIN1"/>
    <dbReference type="Ensembl" id="ENSEAST00005012265.2">
        <property type="protein sequence ID" value="ENSEASP00005011279.2"/>
        <property type="gene ID" value="ENSEASG00005007929.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156871"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15784">
        <property type="entry name" value="PH_RUTBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17703">
        <property type="entry name" value="RUN_SGSM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000004">
        <property type="entry name" value="Small G protein signaling modulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.900:FF:000002">
        <property type="entry name" value="small G protein signaling modulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000006">
        <property type="entry name" value="Small G protein signaling modulator 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.230:FF:000001">
        <property type="entry name" value="Small G protein signaling modulator 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004012">
        <property type="entry name" value="Run_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037213">
        <property type="entry name" value="Run_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047344">
        <property type="entry name" value="RUN_SGSM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037745">
        <property type="entry name" value="SGSM1/2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021935">
        <property type="entry name" value="SGSM1/2_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF187">
        <property type="entry name" value="SMALL G PROTEIN SIGNALING MODULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12068">
        <property type="entry name" value="PH_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02759">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00593">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140741">
        <property type="entry name" value="RUN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50826">
        <property type="entry name" value="RUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RUN" evidence="8">
        <location>
            <begin position="36"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="Rab-GAP TBC" evidence="7">
        <location>
            <begin position="562"/>
            <end position="1027"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="382"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="646"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="84"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="385"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="647"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="687"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="702"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="766"/>
            <end position="776"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011279.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1094" mass="123685" checksum="096A82846CC5A82A" modified="2023-09-13" version="2">MASGPAEAETRQRLLRTVKKEVKQIMEEAVTRKFVHEDSSHIVSLCAAVEACVLHGLRRRAAGFLRSNKIAALFMKVGKSFPPAEELSRKVQDLEQLMENARNQIQGLQENVRKLPKLPNLSPLAIKHLWIRTALFEKVLDKIVHYLVENSSKYYEKEALLMDPVDGPILASLLVGPCALEYTKMKTADHFWTDPSADELVQRHRIHSSHLRQDSPTKRPALCIQKRHSSGSMDDRPSLSARDYVESLHQNSRATLLYGKNNVLVQPRDDMEAVPGYLSLHQTADVMTLKWTPNQLMNGSVGDLDYEKSVYWDYAMTIRLEEIVYLHCHQQVDSGGTVVLVSQDGIQRPPFRFPKGGHLLQFLSCLENGLLPHGQLDPPLWSQRGKGKVFPRLRKRSPQGSAESTSSDKEDDEATDYVFRIIYAGMQSEFVPQDLMDVSVSNLPSLWQPSPRKSSCSSCSQSGSGDGGSTNGCNHERAPLKLLCDNMKYQILSRAFYGWLAYCRHLSTVRTHLSALVNHMIVSPDLPCDAGRGLTAGIWEQYLQDSTSYEEQELLRLIYYGGIQPDIRKAVWPFLLGHYQFGMTETERKEVDEQIHACYAQTMAEWLGCEAIVRQRERESHAAALAKCSSGASLDGHLHRLMHRDSTISNESSQSCSSGRQNVRLQSDSSSSTQVFESVDEVEQVEAEGRPEEKQPKIPNGNLVNGTCSPDSGHPSSHNFSSGLSEHSEPSLSTEDSVMDAQRNAPLMVRPRDSSVDDGQSSEATTSRDEAHREELAVQDSLESDLLANESMDEFMSITGSMDVALPEKEGALMEGWGGSRVEKHSQVDSEDTLSEEPEMESFFPALASLAVTTSANNEASPVSSSGVTYSPELLDLYTVNLHRIEKDVQRCDRNYWYFTPTNLEKLRNIMCSYIWQHIEIGYVQGMCDLLAPLLVILDDEALAFSCFTELMKRMNQNFPHGGAMDTHFANMRSLIQILDSELFELMHQNGDYTHFYFCYRWFLLDFKRELIYDDIFSVWETIWAAKHVSSAHYVLFIALALVEVYRDIILENNMDFTDIIKFFNEMAERHNTKQILKLARDLVYKVQTLIENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LL77</accession>
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        <submittedName>
            <fullName evidence="4">Pleckstrin homology and RhoGEF domain containing G3</fullName>
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        <name evidence="4" type="primary">PLEKHG3</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
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            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LL77"/>
    <dbReference type="Ensembl" id="ENSEAST00005013428.2">
        <property type="protein sequence ID" value="ENSEASP00005012363.2"/>
        <property type="gene ID" value="ENSEASG00005008624.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156521"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0030334">
        <property type="term" value="P:regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:2000114">
        <property type="term" value="P:regulation of establishment of cell polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.20.900.10:FF:000019">
        <property type="entry name" value="Pleckstrin homology domain-containing family G member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45924">
        <property type="entry name" value="FI17866P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45924:SF4">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY G MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00621">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00325">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48065">
        <property type="entry name" value="DBL homology domain (DH-domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="3">
        <location>
            <begin position="92"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="368"/>
            <end position="499"/>
        </location>
    </feature>
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            <begin position="533"/>
            <end position="602"/>
        </location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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        </location>
    </feature>
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            <begin position="1015"/>
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        </location>
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            <begin position="1121"/>
            <end position="1185"/>
        </location>
    </feature>
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        <location>
            <begin position="1208"/>
            <end position="1267"/>
        </location>
    </feature>
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            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
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            <begin position="370"/>
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            <begin position="1243"/>
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    </feature>
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            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="5">
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LLM7</accession>
    <name>A0A8C4LLM7_EQUAS</name>
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        <submittedName>
            <fullName evidence="11">Moesin</fullName>
        </submittedName>
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        <name evidence="11" type="primary">MSN</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
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            <taxon>Mammalia</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="InterPro"/>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
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                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
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                <person name="M."/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="J."/>
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                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LAP (LRR and PDZ) protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LPR8"/>
    <dbReference type="Ensembl" id="ENSEAST00005012123.2">
        <property type="protein sequence ID" value="ENSEASP00005011148.2"/>
        <property type="gene ID" value="ENSEASG00005007851.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000165286"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043194">
        <property type="term" value="C:axon initial segment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016323">
        <property type="term" value="C:basolateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045211">
        <property type="term" value="C:postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045197">
        <property type="term" value="P:establishment or maintenance of epithelial cell apical/basal polarity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098968">
        <property type="term" value="P:neurotransmitter receptor transport postsynaptic membrane to endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098887">
        <property type="term" value="P:neurotransmitter receptor transport, endosome to postsynaptic membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010976">
        <property type="term" value="P:positive regulation of neuron projection development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043113">
        <property type="term" value="P:receptor clustering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06749">
        <property type="entry name" value="PDZ_densin_erbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.42.10:FF:000036">
        <property type="entry name" value="Erbin isoform 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050614">
        <property type="entry name" value="Synaptic_Scaffolding_LAP-MAGUK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119">
        <property type="entry name" value="DISCS LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23119:SF48">
        <property type="entry name" value="LEUCINE-RICH REPEAT-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00595">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="PDZ" evidence="6">
        <location>
            <begin position="734"/>
            <end position="819"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="16"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="414"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="467"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="518"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="616"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="70"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="143"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="156"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="414"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="477"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="527"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="616"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011148.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="821" mass="91533" checksum="0A44E45538CAF837" modified="2023-09-13" version="2">MMKMLGKLRIAQSFPQPLDSKPLLSQREAVPPGNLPQRPDQLPMSDAFTDNWTDGSHYDNTGFVAEETTGENADNNALLNSKSRSTSSHGRRPLIRQDRIVGVPLELEQSTHRHTPETEVPPSNPWQNWTRTPSPFEDRTAFPSKLETTPTTSPLPERKEHIKESAEIPSPFSPGVPWEYHDSNPNRSLSNVFSQIHCRPESSKGVISISKSTERLSPLMKDIKSNKFKKSQSIDEIDIGTYKVYNIPLENYASGSEHLGSHERPDKMLGPEHGMSSMSRSQSVPMLDDEMLTYGGSKGPQQQKASMTKKVYQFDQSFNPQGAVEVKAEKRIPPPFQHNSEYVQQTGKNLAKDLVSPRAYRGYPPMEQMFSFSQPSVNEDAVVNAQFASQGARAGFLRRADSLASSTEMAMFRRVSEPHELPPSDRYGRPPYRGGPDRQGSVSVTESQFLKRNGRYEDEHPSYQEVKAQAGSFPVKNLTQRRPLSARSYSTESYGASQTRPVSARPTMAALLEKIPSDYNLGNYGDKPSDNSDIKTRPTPVKGEESCGKMPADWRQQLLRHIEARRLDRNAAYKHNTVNLGMLPYGGISAMHAGRSMTLNLQTKSKFDLQELPLQKTPSQQSNILDNGQEDVSPSGQWNPYPLGRRDVPPDTITKKAGSHIQTLMGSQSLQHRSREQQPYEGNINKVTIQQFQSPLPIQIPSSQATRGPQPGRCLIQTKGQRSMDGYPEQFCVRIEKNPGLGFSISGGISGQGNPFKPSDKGIFVTRVQPDGPASNLLQPGDKILQANGHSFVHMEHEKAVLLLKSFQNTVDLVIQRELTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LQP6</accession>
    <name>A0A8C4LQP6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Transmembrane protein 230</fullName>
        </recommendedName>
    </protein>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Involved in trafficking and recycling of synaptic vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasmic vesicle</location>
            <location evidence="5">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Secretory vesicle</location>
            <location evidence="3">Synaptic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Early endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus</location>
            <location evidence="6">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Late endosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the TMEM134/TMEM230 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LQP6"/>
    <dbReference type="Ensembl" id="ENSEAST00005014065.2">
        <property type="protein sequence ID" value="ENSEASP00005012940.2"/>
        <property type="gene ID" value="ENSEASG00005009048.2"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008021">
        <property type="term" value="C:synaptic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048489">
        <property type="term" value="P:synaptic vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044234">
        <property type="entry name" value="TMEM230"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008590">
        <property type="entry name" value="TMEM_230/134"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15664">
        <property type="entry name" value="C20ORF30 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15664:SF6">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 230"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05915">
        <property type="entry name" value="TMEM_230_134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0770">Synapse</keyword>
    <keyword evidence="9 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00007743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00024003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00024088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005012940.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="110" mass="12105" checksum="4FAD8615400A3C71" modified="2023-09-13" version="2">MVPTCTNVALGIPSRKVKYSKFSVKRKYLLRFLIRPLPAPLPFLIGAFLFIVGILLLVGSLSKVGANQAILVLIVGILGFLPGFCHLLIAHRAYEGCQGYSYHDLQDCDD</sequence>
</entry>
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    <accession>A0A8C4LXA8</accession>
    <name>A0A8C4LXA8_EQUAS</name>
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        <recommendedName>
            <fullName evidence="4">Nuclear transition protein 2</fullName>
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        <name evidence="15" type="primary">TNP2</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="J."/>
                <person name="Sun"/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
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                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Li"/>
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                <person name="Ju"/>
                <person name="Z."/>
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                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the nuclear transition protein 2 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LXA8"/>
    <dbReference type="Ensembl" id="ENSEAST00005018085.2">
        <property type="protein sequence ID" value="ENSEASP00005016641.2"/>
        <property type="gene ID" value="ENSEASG00005011525.2"/>
    </dbReference>
    <dbReference type="KEGG" id="eai:106836750"/>
    <dbReference type="GeneTree" id="ENSGT00390000008176"/>
    <dbReference type="OrthoDB" id="9809326at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:penetration of zona pellucida"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="8" id="KW-0221">Differentiation</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="44"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
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            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="80"/>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
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    </evidence>
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        </source>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Liu"/>
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                <person name="Wang"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Fan"/>
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                <person name="Li"/>
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                <person name="Li"/>
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                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="Liu"/>
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                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <dbReference type="SMR" id="A0A8C4LXI8"/>
    <dbReference type="Ensembl" id="ENSEAST00005018190.2">
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    <dbReference type="GeneTree" id="ENSGT00740000116858"/>
    <dbReference type="OMA" id="VVEIFCN"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
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    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051287">
        <property type="entry name" value="TCR_variable_region"/>
    </dbReference>
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        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19367">
        <property type="entry name" value="T-CELL RECEPTOR ALPHA CHAIN V REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-0391">Immunity</keyword>
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        <location>
            <begin position="1"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005016738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="94" mass="10308" checksum="BE518A24225AEF92" modified="2022-01-19" version="1">KDQVLQPSTVVSSEGAVAEISCNHSISTAYNFFWYLHLPGCAPRLLIKGSSPSQQGRYNMTYERFSSSLLILQVQVADAATYYCALEDTEAGNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M186</accession>
    <name>A0A8C4M186_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Complement component C7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="29" type="primary">C7</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis. The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways. The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. C7 serves as a membrane anchor. During MAC assembly, associates with C5b and C6 to form the C5b-7 complex, a key lipophilic precursor of the MAC complex, which associates with the outer leaflet and reduces the energy for membrane bending.</text>
    </comment>
    <comment type="subunit">
        <text evidence="22">Monomer or dimer; as a C5b-7 complex it can also form multimeric rosettes. Component of the membrane attack complex (MAC), composed of complement C5b, C6, C7, C8A, C8B, C8G and multiple copies of the pore-forming subunit C9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Target cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complement C6/C7/C8/C9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4M186"/>
    <dbReference type="Ensembl" id="ENSEAST00005021936.2">
        <property type="protein sequence ID" value="ENSEASP00005020204.2"/>
        <property type="gene ID" value="ENSEASG00005013918.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156804"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005579">
        <property type="term" value="C:membrane attack complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044218">
        <property type="term" value="C:other organism cell membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902495">
        <property type="term" value="C:transmembrane transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006957">
        <property type="term" value="P:complement activation, alternative pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006958">
        <property type="term" value="P:complement activation, classical pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160257">
        <property type="term" value="P:complement activation, GZMK pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031640">
        <property type="term" value="P:killing of cells of another organism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00112">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000075">
        <property type="entry name" value="Complement component C7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000077">
        <property type="entry name" value="Complement component C7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.400.10:FF:000099">
        <property type="entry name" value="Complement component C7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.400.10">
        <property type="entry name" value="Low-density Lipoprotein Receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.100.10">
        <property type="entry name" value="Thrombospondin type-1 (TSP1) repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048827">
        <property type="entry name" value="C7_FIM2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048825">
        <property type="entry name" value="C7_KAZAL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003884">
        <property type="entry name" value="FacI_MAC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040729">
        <property type="entry name" value="Kazal_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036055">
        <property type="entry name" value="LDL_receptor-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023415">
        <property type="entry name" value="LDLR_class-A_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002172">
        <property type="entry name" value="LDrepeatLR_classA_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001862">
        <property type="entry name" value="MAC_perforin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020864">
        <property type="entry name" value="MACPF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020863">
        <property type="entry name" value="MACPF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000884">
        <property type="entry name" value="TSP1_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036383">
        <property type="entry name" value="TSP1_rpt_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742">
        <property type="entry name" value="COMPLEMENT COMPONENT C6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45742:SF2">
        <property type="entry name" value="COMPLEMENT COMPONENT C7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21284">
        <property type="entry name" value="C7_FIM2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18434">
        <property type="entry name" value="Kazal_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21330">
        <property type="entry name" value="Kazal_C7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00057">
        <property type="entry name" value="Ldl_recept_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01823">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00090">
        <property type="entry name" value="TSP_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00764">
        <property type="entry name" value="COMPLEMENTC9"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01705">
        <property type="entry name" value="TSP1REPEAT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00057">
        <property type="entry name" value="FIMAC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00192">
        <property type="entry name" value="LDLa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00457">
        <property type="entry name" value="MACPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00209">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57424">
        <property type="entry name" value="LDL receptor-like module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82895">
        <property type="entry name" value="TSP-1 type 1 repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01209">
        <property type="entry name" value="LDLRA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50068">
        <property type="entry name" value="LDLRA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00279">
        <property type="entry name" value="MACPF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51412">
        <property type="entry name" value="MACPF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50092">
        <property type="entry name" value="TSP1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0179">Complement alternate pathway</keyword>
    <keyword evidence="13" id="KW-0180">Complement pathway</keyword>
    <keyword evidence="11" id="KW-0204">Cytolysis</keyword>
    <keyword evidence="16 24" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0473">Membrane attack complex</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0964">Secreted</keyword>
    <keyword evidence="9 26" id="KW-0732">Signal</keyword>
    <keyword evidence="8 24" id="KW-0768">Sushi</keyword>
    <keyword evidence="6" id="KW-1052">Target cell membrane</keyword>
    <keyword evidence="19" id="KW-1053">Target membrane</keyword>
    <feature type="signal peptide" evidence="26">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040263945" description="Complement component C7" evidence="26">
        <location>
            <begin position="23"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="domain" description="MACPF" evidence="28">
        <location>
            <begin position="68"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="27">
        <location>
            <begin position="516"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="Sushi" evidence="27">
        <location>
            <begin position="576"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="464"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="23">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="24">
        <location>
            <begin position="546"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00073222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00093281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00093478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020204.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="790" mass="86727" checksum="BFECF8D0BE53608A" modified="2023-09-13" version="2">MKAISLLILVGFIGEFQVFTSASSPVNCQWASYGPWSECSGCTKTQTRRRSIAVYGQYAGHPCVGSAFETQSCEPTRGCPTEEGCGERFRCFSGQCISKSLVCNGDSDCEEDSADEDRCEDSESRPSCDLDKPPPNIELTGNGYNALTGQFKNRVINTKSFGGQCRKVFSGDRKDFYRLSGNILSYTFQAIVYSYQLLVIQNTVEVAQFINNNPEFLQLAEPFWKELSYLPSLYDYSAYRRLIDRYGTHYLQSGSLGGEYKVLFYVDSEKMKQSGISSADEMECTSSDVNLIFKSSKHKCQALKEALKSAAGTEGNVLRGKPFVRGGSAGFVSGLSYLELDNPAGNKNRYSSWAGSVTDLPQVIKQKLTPLYELVKEVPCASVKRLYLKRALEEYLDEFDPCHCQPCQNGGIATVEGTQCQCHCKPYTFGVACERGVLVGDQAGGVDGDWSCWSSWGPCVQGKKTRRRECNNPPPSRGGKSCIGETSESRQCEDEELKHFRLLEPHCFPLSLVPTEFCSSPPALKDGFVQDEGAMFPVGKNIVYTCNEGYSLVGDPVARCGEDLQWLVGKMHCQKIACVLPALTDGIQSHPHKPFYTIGEKVTFSCSGGMSLEGPSAFLCGSSLKWSPEIKNVRCVQKGAPSTHTVPACQRWEKLKNSRCVCKMPYECGSSLDVCARDEKTKRILPLTVCKMHVLRCQGRNYTLTGRESCSLPASAEKACGVCPLWGKCDDQSSKCVCREAPECEEEGFSVCVEVNGKEQTMTECEAGTLRCGGQSISVTSITPCAAGTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M1I5</accession>
    <name>A0A8C4M1I5_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Chromosome 5 open reading frame 34</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">C5orf34</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M1I5"/>
    <dbReference type="Ensembl" id="ENSEAST00005021293.2">
        <property type="protein sequence ID" value="ENSEASP00005019627.2"/>
        <property type="gene ID" value="ENSEASG00005013488.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00500000044987"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053901">
        <property type="entry name" value="C5orf34-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053899">
        <property type="entry name" value="C5orf34-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027865">
        <property type="entry name" value="C5orf34-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053900">
        <property type="entry name" value="C5orf34-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027830">
        <property type="entry name" value="C5orf34-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34531:SF1">
        <property type="entry name" value="CHROMOSOME 5 OPEN READING FRAME 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34531">
        <property type="entry name" value="ZGC:153352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15025">
        <property type="entry name" value="C5orf34-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22833">
        <property type="entry name" value="C5orf34_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15016">
        <property type="entry name" value="C5orf34_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22834">
        <property type="entry name" value="Polo_box_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C5orf34-like N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="C5orf34-like second" evidence="3">
        <location>
            <begin position="120"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="domain" description="C5orf34-like" evidence="4">
        <location>
            <begin position="334"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="C5orf34-like C-terminal" evidence="1">
        <location>
            <begin position="450"/>
            <end position="539"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF15016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF15025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005019627.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="638" mass="72800" checksum="B58853EFA80BC02A" modified="2023-09-13" version="2">MAAEVRMVLYEDDSVQVQYVDGSRLQLSPCGSEFLFEKAPPVSAHPLEQPERIRQRTHFVISTYREQLQRALDFRNSSATCPFLSDSIIPSEKKKRIFIDVSEVRWPSLDTDDGVIRMQSGTVKISSLDGHAYLCLPKSQQEFTVQFLCKVSQKPDSSRVLSEKNNQAKKDKPVEKAGKICTYGNLSGQRLKNKENELHCQILKPRDPLEKKSCVNGTKGREELPSSGTKYTCVYTWVKQCWSVASCPEEWKYPLSLALRFHNKISNMSRIDVNITQSGILTYDVSEERGKEVSVLPRALLLSCPAPHLHRWNFCDSLSQRQSEEEEYSYPEPVKVVWYQGITYRLTHKNVNSIEIYPGDGSIFKSEGAYFGNYFTYYSIQEGSEEREEKTYSVNNLPPDRPGSLFSVRSLIKQATRILQHCAKMRLSLSHNYRVCCWKMVPGINDSNILPVLLRESLIPSVGRFLAYSDDKVHAIFLDGVTLTLNWNFASFTEKGQVNRGLQLGWCKLTFPDGQGQLIQVEHPGPYERYVAAVILWCRRLAQTSQREQPRRPSSSILEENWSVASELEKIQKFNLLLENSGILNQTAKKTNEQSSDQYEPKSSETVLEVNENRVLAALKKTSEILQDIDCLLSNSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M386</accession>
    <name>A0A8C4M386_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Lymphocyte cytosolic protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">SH2 domain-containing leukocyte protein of 76 kDa</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">SLP-76 tyrosine phosphoprotein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LCP2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Adapter protein primarily involved in signaling pathways within T-cells, as well as other immune cells such as platelets, mast cells, and natural killer (NK) cells. Plays a crucial role for transducing signal from the T-cell receptor (TCR) after antigen recognition leading to T-cell activation. Mechanistically, once phosphorylated by the kinase ZAP70, mediates interactions with the guanine-nucleotide exchange factor VAV1, the adapter protein NCK and the kinase ITK. In turn, stimulates the activation of PKC-theta/PRKCQ and NF-kappa-B transcriptional activity in response to CD3 and CD28 costimulation. Also plays an essential role in AGER-induced signaling pathways including p38 MAPK and ERK1/2 activation leading to cytokine release and pro-inflammatory responses.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with SLA. Interacts with CBLB. Interacts with GRB2. Interacts with SHB. Interacts with PRAM1. Interacts (via SH2 domain) with CD6 (via tyrosine phosphorylated C-terminus). Interacts with FYB1 and the phosphorylated form of FYB2. Interacts with 14-3-3 adapter/YWHAZ; this phosphorylation leads to YWHAZ proteolytic degradation. Interacts with VAV1; this interaction plays a role in TCR-mediated cytokine production. Interacts with AGER; this interaction plays an important role in AGER-mediated pro-inflammatory responses and cytokine release.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014706366.1">
        <property type="nucleotide sequence ID" value="XM_014850880.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M386"/>
    <dbReference type="Ensembl" id="ENSEAST00005021229.2">
        <property type="protein sequence ID" value="ENSEASP00005019568.2"/>
        <property type="gene ID" value="ENSEASG00005013437.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837366"/>
    <dbReference type="KEGG" id="eai:106837366"/>
    <dbReference type="CTD" id="3937"/>
    <dbReference type="GeneTree" id="ENSGT00940000156835"/>
    <dbReference type="OrthoDB" id="9934029at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005911">
        <property type="term" value="C:cell-cell junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044853">
        <property type="term" value="C:plasma membrane raft"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036398">
        <property type="term" value="C:TCR signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007169">
        <property type="term" value="P:cell surface receptor protein tyrosine kinase signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045576">
        <property type="term" value="P:mast cell activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050852">
        <property type="term" value="P:T cell receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd09522">
        <property type="entry name" value="SAM_SLP76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09929">
        <property type="entry name" value="SH2_BLNK_SLP-76"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.505.10:FF:000016">
        <property type="entry name" value="B-cell linker protein isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.50:FF:000051">
        <property type="entry name" value="Lymphocyte cytosolic protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.505.10">
        <property type="entry name" value="SH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.50">
        <property type="entry name" value="Transcription Factor, Ets-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051751">
        <property type="entry name" value="Immunoreceptor_sig_adapters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001660">
        <property type="entry name" value="SAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013761">
        <property type="entry name" value="SAM/pointed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000980">
        <property type="entry name" value="SH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036860">
        <property type="entry name" value="SH2_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14098:SF1">
        <property type="entry name" value="LYMPHOCYTE CYTOSOLIC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14098">
        <property type="entry name" value="SH2 DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07647">
        <property type="entry name" value="SAM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00017">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00454">
        <property type="entry name" value="SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00252">
        <property type="entry name" value="SH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47769">
        <property type="entry name" value="SAM/Pointed domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55550">
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    <sequence length="374" mass="42502" checksum="1655821BED13DA81" modified="2023-09-13" version="2">METSGEGSNPSNRATPRPLAQLQELETRVARRRLSQAHHRATLAGLFSNLRKTVYSQSDLTASKWQVLNKAKNHIQELEQTLDNLLKLKEYFSLKDGNANSLEEVKEEYASMYSRNHSLISNKFHQKGFTTWYPVETVGKDAEEEEKEEEEEEDQEEEEEEEDEEEDEEEEEKNVELLHSPVTLLPDLMEFERYLNFYKQMMDLLIESGIVSSQEVTLPIVSAAISHLWQNLSEERKASLLQAWAQKHSGLPGLTGACQEPACAEGSVKDSGVDSQGASCSLVSTPEEILFEDAFDVASFLDKSEAPSTSSSSSMFASCNPENPEEKFQLYMQIIDFFKGLCCVNTQFKQEPDLPMDDEMIMLRCMETFDDEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M5T2</accession>
    <name>A0A8C4M5T2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="24">Diacylglycerol kinase</fullName>
            <shortName evidence="24">DAG kinase</shortName>
            <ecNumber evidence="24">2.7.1.107</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="primary">DGKI</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="27 28" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="27" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="27" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>1,2-di-(9Z-octadecenoyl)-sn-glycerol + ATP = 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40327"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:52333"/>
            <dbReference type="ChEBI" id="CHEBI:74546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:40328"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol + ATP = 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40323"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:75728"/>
            <dbReference type="ChEBI" id="CHEBI:77091"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:40324"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>a 1,2-diacyl-sn-glycerol + ATP = a 1,2-diacyl-sn-glycero-3-phosphate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10272"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58608"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.107"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:10273"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="22">Glycerolipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Lipid metabolism; glycerolipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
            <location evidence="4">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 24">Belongs to the eukaryotic diacylglycerol kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.107" evidence="24"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4M5T2"/>
    <dbReference type="Ensembl" id="ENSEAST00005022416.2">
        <property type="protein sequence ID" value="ENSEASP00005020645.2"/>
        <property type="gene ID" value="ENSEASG00005011706.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158094"/>
    <dbReference type="UniPathway" id="UPA00230"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042995">
        <property type="term" value="C:cell projection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098978">
        <property type="term" value="C:glutamatergic synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032045">
        <property type="term" value="C:guanyl-nucleotide exchange factor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098794">
        <property type="term" value="C:postsynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098685">
        <property type="term" value="C:Schaffer collateral - CA1 synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004143">
        <property type="term" value="F:ATP-dependent diacylglycerol kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005095">
        <property type="term" value="F:GTPase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060079">
        <property type="term" value="P:excitatory postsynaptic potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046486">
        <property type="term" value="P:glycerolipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046959">
        <property type="term" value="P:habituation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007269">
        <property type="term" value="P:neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007200">
        <property type="term" value="P:phospholipase C-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046579">
        <property type="term" value="P:positive regulation of Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099171">
        <property type="term" value="P:presynaptic modulation of chemical synaptic transmission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900452">
        <property type="term" value="P:regulation of long-term synaptic depression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051966">
        <property type="term" value="P:regulation of synaptic transmission, glutamatergic"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd20850">
        <property type="entry name" value="C1_DGKiota_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20896">
        <property type="entry name" value="C1_DGKiota_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000061">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.40:FF:000002">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10330:FF:000002">
        <property type="entry name" value="Diacylglycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10330">
        <property type="entry name" value="Probable inorganic polyphosphate/atp-NAD kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017438">
        <property type="entry name" value="ATP-NAD_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047486">
        <property type="entry name" value="C1_DGKiota_rpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047487">
        <property type="entry name" value="C1_DGKiota_rpt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037607">
        <property type="entry name" value="DGK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056383">
        <property type="entry name" value="DGKI-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000756">
        <property type="entry name" value="Diacylglycerol_kin_accessory"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001206">
        <property type="entry name" value="Diacylglycerol_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016064">
        <property type="entry name" value="NAD/diacylglycerol_kinase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255">
        <property type="entry name" value="DIACYLGLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11255:SF92">
        <property type="entry name" value="DIACYLGLYCEROL KINASE IOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00609">
        <property type="entry name" value="DAGK_acc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00781">
        <property type="entry name" value="DAGK_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23578">
        <property type="entry name" value="DGKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00045">
        <property type="entry name" value="DAGKa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00046">
        <property type="entry name" value="DAGKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111331">
        <property type="entry name" value="NAD kinase/diacylglycerol kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50146">
        <property type="entry name" value="DAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 23" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="13 24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="18" id="KW-0966">Cell projection</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 24" id="KW-0418">Kinase</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="11 24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="9 24" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAGKc" evidence="26">
        <location>
            <begin position="375"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="23">
        <location>
            <begin position="974"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="23">
        <location>
            <begin position="1010"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="35"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="333"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1038"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="25">
        <location>
            <begin position="35"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="25">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="25">
        <location>
            <begin position="340"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="1038"/>
            <end position="1048"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00060536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU361128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020645.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1081" mass="118953" checksum="0D3F69C919FF5F22" modified="2023-09-13" version="2">MDAAGRGCHLLPLPVARGPAGSPAAAAASAVSRPGLCGAAASPPSAAAGAVAMNPSSSAGEEKGATGGSSSGGSGAGSCCLGAEGGADPRGARGRAAAGPPGGPGLPWPAGAGPKEKDEALEEKLRNLTFRKQVSYRKAISRAGLQHLAPAHPLSLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGTEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDIHQVQAADLRRVSAPPGSFTIPQSVPDRLRIRVNKIGLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVLSQQAGTPPGMPDLVVEQASGMSDWWNPALRKRMLSDSGLGMITPHYEDSDLKDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQDRAQQAGDPDLAAYLESRLNYKIIGHEDLETAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M7P2</accession>
    <name>A0A8C4M7P2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box only protein 39</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">FBXO39</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Directly interacts with SKP1 and CUL1.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014688174.1">
        <property type="nucleotide sequence ID" value="XM_014832688.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044601101.1">
        <property type="nucleotide sequence ID" value="XM_044745166.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044601102.1">
        <property type="nucleotide sequence ID" value="XM_044745167.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044601103.1">
        <property type="nucleotide sequence ID" value="XM_044745168.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070338630.1">
        <property type="nucleotide sequence ID" value="XM_070482529.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070338631.1">
        <property type="nucleotide sequence ID" value="XM_070482530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M7P2"/>
    <dbReference type="Ensembl" id="ENSEAST00005024321.2">
        <property type="protein sequence ID" value="ENSEASP00005022411.1"/>
        <property type="gene ID" value="ENSEASG00005015321.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106825724"/>
    <dbReference type="KEGG" id="eai:106825724"/>
    <dbReference type="CTD" id="162517"/>
    <dbReference type="GeneTree" id="ENSGT00510000048837"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd22108">
        <property type="entry name" value="F-box_FBXO39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.50:FF:000027">
        <property type="entry name" value="F-box only protein 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000237">
        <property type="entry name" value="F-box only protein 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045048">
        <property type="entry name" value="FBXO31/39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10706">
        <property type="entry name" value="F-BOX FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10706:SF160">
        <property type="entry name" value="F-BOX ONLY PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="13"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00062469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00068850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005022411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="443" mass="52704" checksum="C88F712E9BB1DFED" modified="2022-01-19" version="1">MDEESQLLQPQEQSCWAALPDVCLRRVFWWLGDRDRSRAALVCRKWNQTMYSADLWRYRTITFSGRPSRVHASEFESALWYVKKFGRYLEHLEIKFLNPYNAVLTKKFQITMRGLLSCLGKSNNRLKSLSIQHLELDRLVWRNSIRTSFIKSLSFFLKKTGKHLDYLNLKGARLTVEQGCHVLNSLSYLRNESMASELNIEDYFSHHLAIYSSPQFNKTMATFRNLASLTLNYNCLSDELLENLCENNAGTLWTINIKCHIHDPHGQVIWGMSWANLARHATNLKVNFFFERVMKYERLARILLQEIPIRSISLRSCYFSDPDWSMRPTLVDLLPTFRHTLQKLTFEFNNNHESLDDELHLLILSCRKLFYFKIWAFLDVRFVERILKSQEEGQCALRTLKVRIYTNRYETNEEDKTLREIYRKYRKLIDSELNYFVIAYPMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MBX5</accession>
    <name>A0A8C4MBX5_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Meis homeobox 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">MEIS2</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TALE/MEIS homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044621121.1">
        <property type="nucleotide sequence ID" value="XM_044765186.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MBX5"/>
    <dbReference type="Ensembl" id="ENSEAST00005026690.2">
        <property type="protein sequence ID" value="ENSEASP00005024610.1"/>
        <property type="gene ID" value="ENSEASG00005016725.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835157"/>
    <dbReference type="CTD" id="4212"/>
    <dbReference type="GeneTree" id="ENSGT00940000155643"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001654">
        <property type="term" value="P:eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045638">
        <property type="term" value="P:negative regulation of myeloid cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110024">
        <property type="term" value="P:positive regulation of cardiac muscle myoblast proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045931">
        <property type="term" value="P:positive regulation of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008542">
        <property type="term" value="P:visual learning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000004">
        <property type="entry name" value="Meis2 homeobox isoform 2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032453">
        <property type="entry name" value="PKNOX/Meis_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16493">
        <property type="entry name" value="Meis_PKNOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="7">
        <location>
            <begin position="274"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="276"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="193"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="193"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="239"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005024610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="477" mass="51774" checksum="94E30B601A374AC4" modified="2022-01-19" version="1">MAQRYDELPHYGGMDGVGVPASMYGDPHAPRPIPPVHHLNHGPPLHATQHYGAHAPHPNVMPASMGSAVNDALKRDKDAIYGHPLFPLLALVFEKCELATCTPREPGVAGGDVCSSDSFNEDIAVFAKQVRAEKPLFSSNPELDNLMIQAIQVLRFHLLELEKVHELCDNFCHRYISCLKGKMPIDLVIDERDGSSKSDHEELSGSSTNLADHNPASWRDHDDATSTHSAGTPGPSSGGHASQSGDNSSEQGDGLDNSVASPGTGDDDDPDKDKKRQKKRGIFPKVATNIMRAWLFQHLTHPYPSEEQKKQLAQDTGLTILQVNNWFINARRRIVQPMIDQSNRAGFLLDPSVSQGAAYSPEGQPMGSFVLDGQQHMGIRPAGLQSMPGDYVSQGGPMGMSMAQPSYTPPQMTPHPTQLRHGPPMHSYLPSHPHHPAMMMHGGPPTHPGMTMSAQSPTMLNSVDPNVGGQVMDIHAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MDH9</accession>
    <name>A0A8C4MDH9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Enolase-phosphatase E1</fullName>
            <ecNumber evidence="8">3.1.3.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">2,3-diketo-5-methylthio-1-phosphopentane phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">MASA homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">ENOPH1</name>
        <name evidence="8" type="synonym">MASA</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:49252"/>
            <dbReference type="ChEBI" id="CHEBI:58828"/>
            <dbReference type="EC" id="3.1.3.77"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="8">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the HAD-like hydrolase superfamily. MasA/MtnC family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.77" evidence="8"/>
    <dbReference type="RefSeq" id="XP_014720708.1">
        <property type="nucleotide sequence ID" value="XM_014865222.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MDH9"/>
    <dbReference type="Ensembl" id="ENSEAST00005026006.2">
        <property type="protein sequence ID" value="ENSEASP00005023977.1"/>
        <property type="gene ID" value="ENSEASG00005016274.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106846433"/>
    <dbReference type="KEGG" id="eai:106846433"/>
    <dbReference type="CTD" id="58478"/>
    <dbReference type="GeneTree" id="ENSGT00440000039914"/>
    <dbReference type="OMA" id="LQGMVWE"/>
    <dbReference type="OrthoDB" id="272500at2759"/>
    <dbReference type="UniPathway" id="UPA00904">
        <property type="reaction ID" value="UER00876"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043874">
        <property type="term" value="F:acireductone synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01629">
        <property type="entry name" value="HAD_EP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.720.60:FF:000002">
        <property type="entry name" value="Enolase-phosphatase E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000102">
        <property type="entry name" value="Enolase-phosphatase E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.720.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01681">
        <property type="entry name" value="Salvage_MtnC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03117">
        <property type="entry name" value="Salvage_MtnC_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023943">
        <property type="entry name" value="Enolase-ppase_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027511">
        <property type="entry name" value="ENOPH1_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01691">
        <property type="entry name" value="enolase-ppase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20371">
        <property type="entry name" value="ENOLASE-PHOSPHATASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20371:SF1">
        <property type="entry name" value="ENOLASE-PHOSPHATASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01133">
        <property type="entry name" value="C1.5.4:_Enolase-phosphatase_Li"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00044">
        <property type="entry name" value="enolase-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 8" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="153"/>
            <end position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005023977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="261" mass="28920" checksum="10C3455B7C173DF3" modified="2022-01-19" version="1">MVVLSVPAEVTVILLDIEGTTTPIAFVKDILFPYIKENVKEYLQTHWEEEECQEDVGLLRRQAEEDSHLEGAVPIPAVSDNGAADLQQVIQAVVDNVCWQMSLDRKTTALKQLQGHIWRAAFTAGRMKAEFFEDVVPAVRKWREAGMKVYIYSSGSVEAQKLLFGHSTEGDILELFDGHFDTKIGHKVESESYRKIADSIGCSTNNILFLTDVTLEASAAEEADVHVAVVVRPGNAGLTDDEKTYYSLITSFSELDLPASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MEK9</accession>
    <name>A0A8C4MEK9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Synaptobrevin homolog YKT6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">YKT6</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Vesicular soluble NSF attachment protein receptor (v-SNARE) mediating vesicle docking and fusion to a specific acceptor cellular compartment. Functions in endoplasmic reticulum to Golgi transport; as part of a SNARE complex composed of GOSR1, GOSR2 and STX5. Functions in early/recycling endosome to TGN transport; as part of a SNARE complex composed of BET1L, GOSR1 and STX5. Has a S-palmitoyl transferase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20">Identified in 2 different SNARE complexes; the first one composed of GOSR1, GOSR2 and STX5 and the second one composed of BET1L, GOSR1 and STX5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Cytoplasmic vesicle membrane</location>
            <topology evidence="18">Lipid-anchor</topology>
            <orientation evidence="18">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the synaptobrevin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014683824.1">
        <property type="nucleotide sequence ID" value="XM_014828338.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MEK9"/>
    <dbReference type="SMR" id="A0A8C4MEK9"/>
    <dbReference type="Ensembl" id="ENSEAST00005028260.2">
        <property type="protein sequence ID" value="ENSEASP00005026031.1"/>
        <property type="gene ID" value="ENSEASG00005017757.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822931"/>
    <dbReference type="KEGG" id="eai:106822931"/>
    <dbReference type="CTD" id="10652"/>
    <dbReference type="GeneTree" id="ENSGT00390000015164"/>
    <dbReference type="OMA" id="HYIGIIR"/>
    <dbReference type="OrthoDB" id="27923at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd14824">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15867">
        <property type="entry name" value="R-SNARE_YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000020">
        <property type="entry name" value="synaptobrevin homolog YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.50:FF:000013">
        <property type="entry name" value="Synaptobrevin homolog YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.50">
        <property type="entry name" value="Longin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010908">
        <property type="entry name" value="Longin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045848">
        <property type="entry name" value="R-SNARE_YKT6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45806:SF1">
        <property type="entry name" value="SYNAPTOBREVIN HOMOLOG YKT6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13774">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00957">
        <property type="entry name" value="Synaptobrevin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01270">
        <property type="entry name" value="Longin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50859">
        <property type="entry name" value="LONGIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 21" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="10" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="13" id="KW-0564">Palmitate</keyword>
    <keyword evidence="15" id="KW-0636">Prenylation</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Longin" evidence="22">
        <location>
            <begin position="8"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="23">
        <location>
            <begin position="138"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00025256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00025701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00026133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00062919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="198" mass="22304" checksum="25C48C7D50D5DC74" modified="2022-01-19" version="1">MKLYSLSVLYKGEPKAVLLKAAYDVSSFSFFQRSSVQEFMTFTSQLIVERSAKGSRASVKEQEYLCHVYVRNDSLAGVVIADSEYPSRVAFTLLEKVLDEFSKQVDKVEWPVGSPATIHYAALDGHLSRYQNPREADPMSKVQAELDETKIILHNTMESLLERGEKLDDLVSKSEVLGTQSKAFYKTARKQNSCCAIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MG17</accession>
    <name>A0A8C4MG17_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">protein-tyrosine-phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">LOC106845198</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <reaction evidence="10">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
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            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
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        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.</text>
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    <dbReference type="AlphaFoldDB" id="A0A8C4MG17"/>
    <dbReference type="Ensembl" id="ENSEAST00005023920.2">
        <property type="protein sequence ID" value="ENSEASP00005022045.2"/>
        <property type="gene ID" value="ENSEASG00005015049.2"/>
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    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0050839">
        <property type="term" value="F:cell adhesion molecule binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0004726">
        <property type="term" value="F:non-membrane spanning protein tyrosine phosphatase activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0030971">
        <property type="term" value="F:receptor tyrosine kinase binding"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0070374">
        <property type="term" value="P:positive regulation of ERK1 and ERK2 cascade"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="PTPc-N11_6"/>
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        <property type="entry name" value="SH2_C-SH2_SHP_like"/>
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    <sequence length="554" mass="59238" checksum="1E1DD2CF7AA951D0" modified="2023-09-13" version="2">MEPSGPGAEAGMEGAAGDPYQRPARRTQWLLSALAHHYGLDRGVENEIVVLATGLDQYLQEVFHHLDCRGAGRLPRADFRALCAVLGLRAEGAAAAGEATRDAAAGDTSSGNVTAGDAAAGLDTDRDADDEEEARLALSAEPPELTFRQFHARLCGYFGTRAGPRLPRGALSEHIETQIRLRRPRRRRRPPRTPGPDGGPDGGPDSERLARLEEENSSLRELVEDLRAALQSSDARCLALQVGLWKSQAGAPEAAARELRLRGALAAAEARAGRLRQGQAEVRRRAEQARQALQRSVRRVRELEALARRVPGLQRWVRRLEGELRRYRSEGTQLPASPRASPEPEAKSSEPEDCGTRGSDAPPEGAWQSEGSSESRALDEAVGEQLFRSVEGQAASDEEEEDKEKWQEEQRPPAEVKMLLAHLHSCGSGCDAQMAKELTTSFSLFGSTTHAHALGELKAHIAMLVEQLGTQGCSQKTLGSSGQEAELQQKVEENEALEAGAADGGDGASEAVLAGGEAGGCAAAPAEAPGPEHIEESAGEDVAEHTGRLQGPRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MJR2</accession>
    <name>A0A8C4MJR2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Axin-1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Axis inhibition protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">AXIN1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MJR2"/>
    <dbReference type="Ensembl" id="ENSEAST00005028914.2">
        <property type="protein sequence ID" value="ENSEASP00005026631.2"/>
        <property type="gene ID" value="ENSEASG00005018173.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156947"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
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        <property type="term" value="C:beta-catenin destruction complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016328">
        <property type="term" value="C:lateral plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015630">
        <property type="term" value="C:microtubule cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990909">
        <property type="term" value="C:Wnt signalosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008013">
        <property type="term" value="F:beta-catenin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070411">
        <property type="term" value="F:I-SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120283">
        <property type="term" value="F:protein serine/threonine kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070412">
        <property type="term" value="F:R-SMAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035591">
        <property type="term" value="F:signaling adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990756">
        <property type="term" value="F:ubiquitin-like ligase-substrate adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048320">
        <property type="term" value="P:axial mesoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904885">
        <property type="term" value="P:beta-catenin destruction complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048468">
        <property type="term" value="P:cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009950">
        <property type="term" value="P:dorsal/ventral axis specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044725">
        <property type="term" value="P:epigenetic programming in the zygotic pronuclei"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060322">
        <property type="term" value="P:head development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001701">
        <property type="term" value="P:in utero embryonic development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045599">
        <property type="term" value="P:negative regulation of fat cell differentiation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051248">
        <property type="term" value="P:negative regulation of protein metabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034244">
        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase II"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031398">
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    <dbReference type="GO" id="GO:0036342">
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0030163">
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    <dbReference type="GO" id="GO:0007605">
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    <dbReference type="InterPro" id="IPR024066">
        <property type="entry name" value="RGS_subdom1/3"/>
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    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
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    <keyword evidence="12" id="KW-0832">Ubl conjugation</keyword>
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    <feature type="region of interest" description="Disordered" evidence="17">
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            <end position="276"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
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            <end position="390"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="582"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="594"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026631.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1037" mass="114259" checksum="D7FEB04A87D58F74" modified="2023-09-13" version="2">MGQEEDGHGRNPGTQQQLCRTKDRLIHFGAVSTDVLGCSPRCSLTQSPKMSVQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDPRPVSHSFCSGKGPGIKGETSTATPRRSDLDLGYEPEGSASPTPPYLKWAESLHSLLDDQDGINLFRTFLKQEDCADLLDFWFACSGFRKLEPCDSNEEKRLKLAKAIYRKYILDNNGIVSRQTKPATKSFIKDCIMKQLIDPAMFDQAQTEIQSTMEENTYPSFLKSDIYLEYTRTGSESPKLCSDQSSGSGTGKGIPGYLPTLNEDEEWKCDQDVDEDDGRDPGPPGRLTQKLLLETAAPRASASRRYSEGREFRYGSWREPVNPYYVNSGYALAPATSANDSEQQSLSSDADSLSLTDSSVDGVPPYRIRKQHRREMQESVQVNGRVPLPHIPRTYRMPKEIRVEPQKFAAELIHRLEAIQRTREAEEKLEERLKRVRMEEEGEDGDVSCGPPGASHKLPSAPAWHHFPPRYADMGCTGLRDAHEENPESILDEHVQRVMRTPGCQSPGPGHRSPDSVHVPKVPGVLGGIAPGHGKHALKSGAKLDAAGLHLHRHSHHHGHHGLARPKEQAEAEAARRVQSSFSWGLEQHGHTAKPRSHSESVGAAHITSDGLAYSGKAGTTCKRNTKKAESGKSMGAEAPGPSEDAEKNQKIMQWIIEGEKEISRHRKAGHGSSGTKKQQGHESSRPLSIERPGAVHPWVSAQLRNSVQPSHLFIQDPTMPPNPAPNPLTQLEEARRRLEEEEKRASKLPSKQRYVQEVIQRGRSCARPACTPVLSVVPAVSDLELSETETKSQRKVGSSSTQPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGNYRYYFKKVSDEFDCGVVFEEVREDEAVLPVFEEKIIGKVEKVVVPPSGPTGFHPAADEEGARLPSTAILGGAAPMTHPSGPAADHRLAHWRLAGSPHHGLCFSLWDSPPRPALVGTREASVNCTCHRVPSTQLVSCPPLWTWQRSAAVPSLLRPQVPKRRLSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4ML20</accession>
    <name>A0A8C4ML20_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="1">PPIase</shortName>
            <ecNumber evidence="1">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="1"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4ML20"/>
    <dbReference type="Ensembl" id="ENSEAST00005029725.2">
        <property type="protein sequence ID" value="ENSEASP00005027376.1"/>
        <property type="gene ID" value="ENSEASG00005018652.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183087"/>
    <dbReference type="OMA" id="DPRNEIC"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF532">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="2">
        <location>
            <begin position="20"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005027376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="88" mass="10032" checksum="11384648FAFE34DD" modified="2022-01-19" version="1">MIRGEENIKGYPALVSPTMFFHFIVNNEPLDSVSFELFEDKIPKTAENFCVLNTGEKGFDYKAFCFQRIILGSMCQAGDFPCHNGTWQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MM54</accession>
    <name>A0A8C4MM54_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Dolichol-phosphate mannosyltransferase subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">DPM3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Stabilizer subunit of the dolichol-phosphate mannose (DPM) synthase complex; tethers catalytic subunit to the ER.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the dolichol-phosphate mannose (DPM) synthase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Endoplasmic reticulum membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DPM3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014692542.1">
        <property type="nucleotide sequence ID" value="XM_014837056.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014692543.1">
        <property type="nucleotide sequence ID" value="XM_014837057.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MM54"/>
    <dbReference type="Ensembl" id="ENSEAST00005031042.2">
        <property type="protein sequence ID" value="ENSEASP00005028574.1"/>
        <property type="gene ID" value="ENSEASG00005019433.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106828503"/>
    <dbReference type="CTD" id="54344"/>
    <dbReference type="GeneTree" id="ENSGT00390000008892"/>
    <dbReference type="OMA" id="KLMQWLF"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033185">
        <property type="term" value="C:dolichol-phosphate-mannose synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0180047">
        <property type="term" value="P:dolichol phosphate mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013174">
        <property type="entry name" value="DPM3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16433">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 3"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005028841.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="304" mass="34354" checksum="3140546E97B2F669" modified="2023-09-13" version="2">MEKYTESPPALQVEHSSLQAENLILRPQMQYLTEENPAIRGQVMPSLATSMMPVSCSLEHLSQFRDDPASLSGFLAQVTTYLTALKIPNPADDAQVKFFFDYLYQQMKSCGIISGPDKRTLLKQYENFVLEFQQSFRELTKHKMNNLDKGDNSQQDATTFQILAQNLSYNETNQTDHFQEGLADPIQDEVSGTDMVDNLPDLVTQCIQLDRKRSDRPELLQSEPQLPMLASLINHQALSSPIGLSPKEEPIQLRGSQLPLTPAKRARQQETQLCLYCSQSGHFTRDCLAKRSRAPARINDPAHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MQT5</accession>
    <name>A0A8C4MQT5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Succinate dehydrogenase assembly factor 3</fullName>
            <shortName evidence="6">SDH assembly factor 3</shortName>
            <shortName evidence="6">SDHAF3</shortName>
        </recommendedName>
    </protein>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Promotes maturation of the iron-sulfur protein subunit of the SDH catalytic dimer, protecting it from the deleterious effects of oxidants. May act together with SDHAF1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Interacts with the iron-sulfur protein subunit within the SDH catalytic dimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the complex I LYR family. SDHAF3 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MQT5"/>
    <dbReference type="Ensembl" id="ENSEAST00005032814.2">
        <property type="protein sequence ID" value="ENSEASP00005030184.1"/>
        <property type="gene ID" value="ENSEASG00005020547.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000010029"/>
    <dbReference type="OMA" id="WSSHCRE"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034553">
        <property type="term" value="P:mitochondrial respiratory chain complex II assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006105">
        <property type="term" value="P:succinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd20270">
        <property type="entry name" value="Complex1_LYR_SDHAF3_LYRM10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008381">
        <property type="entry name" value="SDHAF3/Sdh7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137">
        <property type="entry name" value="DC11 ACN9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13137:SF6">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13233">
        <property type="entry name" value="Complex1_LYR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 6" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034163684" description="Succinate dehydrogenase assembly factor 3" evidence="8">
        <location>
            <begin position="24"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="110"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="141"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="159" mass="17580" checksum="FA93E91E9545B546" modified="2022-01-19" version="1">MQCYASLACLTGLGLVLVPLAAAQGSAPELKTPGDQYMKDEFRRHKTVGSEEAQCFLQEWEVYAAVLWQQANENRQNSTEKACFGTTLPEEKLSDFRDEQTGQLQELMQEASKPNRQFSGTELLPKYSGGAGDTPGPGEKWSSHCREGLAFKKKKKGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MR70</accession>
    <name>A0A8C4MR70_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Zinc finger protein 467</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ZNF467</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Transcription factor that promotes adipocyte differentiation and suppresses osteoblast differentiation in the bone marrow. Enhances the osteoclast-supporting ability of stromal cells. Binds with STAT3 the consensus sequence 5'-CTTCTGGGAAGA-3' of the acute phase response element (APRE). Transactivates several promoters including FOS, OSM and PPARG. Recruits a histone deacetylase complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with STAT3. Enhances STAT3 activity by keeping it in the nucleus.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MR70"/>
    <dbReference type="Ensembl" id="ENSEAST00005032999.2">
        <property type="protein sequence ID" value="ENSEASP00005030352.2"/>
        <property type="gene ID" value="ENSEASG00005020669.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162497"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000214">
        <property type="entry name" value="replication initiator 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000750">
        <property type="entry name" value="replication initiator 1 isoform X2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001598">
        <property type="entry name" value="Zinc finger protein 467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001683">
        <property type="entry name" value="Zinc finger protein 467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001789">
        <property type="entry name" value="zinc finger protein 467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001800">
        <property type="entry name" value="Zinc finger protein 467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001993">
        <property type="entry name" value="Zinc finger protein 467"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000320">
        <property type="entry name" value="Zinc finger protein 777"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF50">
        <property type="entry name" value="GASTRULA ZINC FINGER PROTEIN XLCGF57.1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="12"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="12"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 16" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="161"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="217"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="245"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="273"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="301"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="353"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="421"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="449"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="477"/>
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        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="505"/>
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        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="18">
        <location>
            <begin position="533"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="316"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="330"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00053501"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063249"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00067785"/>
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    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030352.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="585" mass="64213" checksum="A6F9984D1AF79821" modified="2023-09-13" version="2">MRETLEALSSLGFSVGQPEMAPQSEPGERSHHAQGQMSSPQEDRALGMGAGHEAPRLEEGAHTEQAEAPCRGGQVRTARKAEPTGSCPGDEWMTRKVKVEKEDQEAEEEVEWPQHLSLLPGPFPAPDLGPLAAAYKLEPGAPGALGGLALAGWAPASEKPYGCGECERRFRDQLTLRLHQRLHRGEGPCACPDCGRSFTQRAHMLMHQRSHRGERPFPCSECDKRFSKKAHLTRHLRTHTGERPYPCAECGKRFSQKIHLGSHQKTHTGERPFPCTECEKRFRKKTHLIRHQRIHTGERPYQCAQCARSFTHKQHLVRHQRVHEAAGRTPPSPDVPSSPAPSPTPSPPGPKPFACSDCGLSFGWKKNLATHQRLHRSEGRPFGCEECALGATVDPAAAEPLAGVPRSTAAPQGAPASERSLFCPDCGRGFAHGQHLARHRRVHTGERPFACAQCGRRFGSRPNLVAHSRAHSGARPFACTQCGRRFSRKSHLGRHQAVHTGSRPHSCAICARSFSSKTNLVRHQAIHTGSRPFSCPQCGKSFSRKTHLVRHQRIHGDVAHPASDADLSAPAWPTAAEVAAPPLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MS09</accession>
    <name>A0A8C4MS09_EQUAS</name>
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        <recommendedName>
            <fullName evidence="7">Alpha/beta-tubulin-N-acetyltransferase 9</fullName>
            <ecNumber evidence="6">2.3.1.308</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">NAT9</name>
    </gene>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
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                <person name="S."/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="G."/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">N-acetyltransferase that mediates the acetylation of the N-terminal residues of alpha- and beta-tubulin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>N-terminal L-methionyl-[tubulin] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-[tubulin] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:69607"/>
            <dbReference type="Rhea" id="RHEA-COMP:17729"/>
            <dbReference type="Rhea" id="RHEA-COMP:17730"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="ChEBI" id="CHEBI:133414"/>
            <dbReference type="EC" id="2.3.1.308"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetyltransferase family. GNAT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.308" evidence="6"/>
    <dbReference type="RefSeq" id="XP_014695232.1">
        <property type="nucleotide sequence ID" value="XM_014839746.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014695235.1">
        <property type="nucleotide sequence ID" value="XM_014839749.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014695236.1">
        <property type="nucleotide sequence ID" value="XM_014839750.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MS09"/>
    <dbReference type="Ensembl" id="ENSEAST00005030417.2">
        <property type="protein sequence ID" value="ENSEASP00005028006.2"/>
        <property type="gene ID" value="ENSEASG00005019038.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106830268"/>
    <dbReference type="KEGG" id="eai:106830268"/>
    <dbReference type="CTD" id="26151"/>
    <dbReference type="GeneTree" id="ENSGT00390000012745"/>
    <dbReference type="OrthoDB" id="5043642at2759"/>
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        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120519">
        <property type="term" value="F:tubulin N-terminal-methionine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000040">
        <property type="entry name" value="N-acetyltransferase 9 (putative)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039135">
        <property type="entry name" value="NAT9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13256:SF16">
        <property type="entry name" value="ALPHA_BETA-TUBULIN-N-ACETYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13256">
        <property type="entry name" value="N-ACETYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="8">
        <location>
            <begin position="14"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="206" mass="23604" checksum="7B600F39B3380E84" modified="2023-09-13" version="2">MRLNQNILLLGKKVALVPYTSEHVPRYHEWMKSEELQRLTASEPLTLEQEYAMQHSWREDADKCTFIVLDAEKWQAQPAATEESCMVGDVNLFLTDRGDPSLGEIEVMIAEPSCRGKGFGTEAVLMMLSYGVTRLGLTKFEAKIGEGNEPSIQMFRKLHFEQVAVSTVFQEVTLRLMMSEPERQWLLQQTSHVEEKPYRDGSLEPH</sequence>
</entry>
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    <accession>A0A8C4MUW8</accession>
    <name>A0A8C4MUW8_EQUAS</name>
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        <submittedName>
            <fullName evidence="5">Coiled-coil domain containing 42</fullName>
        </submittedName>
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    <gene>
        <name evidence="5" type="primary">CCDC42</name>
    </gene>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Metazoa</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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                <person name="X. and Zhong"/>
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleoplasm</location>
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    <dbReference type="SMR" id="A0A8C4MW20"/>
    <dbReference type="Ensembl" id="ENSEAST00005031287.2">
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    <accession>A0A8C4MXY5</accession>
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        <recommendedName>
            <fullName evidence="5">Ras suppressor protein 1</fullName>
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        <name evidence="8" type="primary">RSU1</name>
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        <name type="scientific">Equus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
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                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="4">Potentially plays a role in the Ras signal transduction pathway. Capable of suppressing v-Ras transformation in vitro.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="Ras suppressor protein 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ras suppressor protein 1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
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        <property type="entry name" value="Leu-rich_rpt"/>
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        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
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        <property type="entry name" value="RAS SUPPRESSOR PROTEIN 1"/>
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    <dbReference type="Pfam" id="PF00560">
        <property type="entry name" value="LRR_1"/>
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        <property type="entry name" value="LRR_14"/>
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    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LRR_BAC"/>
        <property type="match status" value="5"/>
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    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="7"/>
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    <dbReference type="SUPFAM" id="SSF52058">
        <property type="entry name" value="L domain-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="LRR"/>
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    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
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    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Disease resistance R13L4/SHOC-2-like LRR" evidence="7">
        <location>
            <begin position="110"/>
            <end position="203"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="7"/>
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</entry>
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        <submittedName>
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        <name evidence="2" type="primary">IL9</name>
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            <taxon>Mammalia</taxon>
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        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_014683893.2">
        <property type="nucleotide sequence ID" value="XM_014828407.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A8C4N0C2"/>
    <dbReference type="Ensembl" id="ENSEAST00005032745.2">
        <property type="protein sequence ID" value="ENSEASP00005030129.1"/>
        <property type="gene ID" value="ENSEASG00005020504.2"/>
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        <property type="component" value="Chromosome 12"/>
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        <property type="term" value="C:extracellular space"/>
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    <dbReference type="GO" id="GO:0005140">
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        <property type="term" value="P:B cell differentiation"/>
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    <dbReference type="GO" id="GO:0042100">
        <property type="term" value="P:B cell proliferation"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0016064">
        <property type="term" value="P:immunoglobulin mediated immune response"/>
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        <property type="term" value="P:positive regulation of interleukin-5 production"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0046425">
        <property type="term" value="P:regulation of receptor signaling pathway via JAK-STAT"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="InterPro" id="IPR020447">
        <property type="entry name" value="IL-9"/>
    </dbReference>
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        <property type="entry name" value="INTERLEUKIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="INTERLEUKIN-9"/>
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    <dbReference type="PRINTS" id="PR01926">
        <property type="entry name" value="INTERLEUKIN9"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034281393" evidence="1">
        <location>
            <begin position="19"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="147" mass="16128" checksum="31DA2C198B5ABB52" modified="2022-01-19" version="1">MLLVVVLASALLLCSVAGQGCSTTAGIMDVKYLIDNLQEHPPSKCSCSTNVTDCLCLPIPSDDCTTACFQEGLSQMTNTTVKTRFALIFNRVKKTVAALKNNKCQLFSCEQPCNQTTRGNTVTFLKSLLEIFQKKRIRDAETQRGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N2R9</accession>
    <name>A0A8C4N2R9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Endoribonuclease Dicer</fullName>
            <ecNumber evidence="5">3.1.26.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="31" type="primary">DICER1</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="21">Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="22 23">Belongs to the helicase family. Dicer subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.3" evidence="5"/>
    <dbReference type="Ensembl" id="ENSEAST00005034302.2">
        <property type="protein sequence ID" value="ENSEASP00005031559.2"/>
        <property type="gene ID" value="ENSEASG00005021485.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 7"/>
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        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016442">
        <property type="term" value="C:RISC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070578">
        <property type="term" value="C:RISC-loading complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004530">
        <property type="term" value="F:deoxyribonuclease I activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070883">
        <property type="term" value="F:pre-miRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019904">
        <property type="term" value="F:protein domain specific binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035197">
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:apoptotic DNA fragmentation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:global gene silencing by mRNA cleavage"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:negative regulation of tumor necrosis factor production"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:negative regulation of tumor necrosis factor-mediated signaling pathway"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:RISC complex assembly"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:siRNA processing"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="CDD" id="cd18034">
        <property type="entry name" value="DEXHc_dicer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15903">
        <property type="entry name" value="Dicer_PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10843">
        <property type="entry name" value="DSRM_DICER"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd02843">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd18802">
        <property type="entry name" value="SF2_C_dicer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000023">
        <property type="entry name" value="Endoribonuclease dcr-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000015">
        <property type="entry name" value="endoribonuclease Dicer"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.40.50.300:FF:000628">
        <property type="entry name" value="Endoribonuclease Dicer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.380:FF:000002">
        <property type="entry name" value="Endoribonuclease Dicer isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000588">
        <property type="entry name" value="Endoribonuclease Dicer isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000005">
        <property type="entry name" value="Putative endoribonuclease dicer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.260.10:FF:000002">
        <property type="entry name" value="Putative Endoribonuclease Dicer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.380">
        <property type="entry name" value="Dicer dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038248">
        <property type="entry name" value="Dicer_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005034">
        <property type="entry name" value="Dicer_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044441">
        <property type="entry name" value="DICER_DSRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048513">
        <property type="entry name" value="Dicer_PBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048512">
        <property type="entry name" value="Dicer_platform"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014720">
        <property type="entry name" value="dsRBD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950">
        <property type="entry name" value="DICER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950:SF37">
        <property type="entry name" value="ENDORIBONUCLEASE DICER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03368">
        <property type="entry name" value="Dicer_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20932">
        <property type="entry name" value="Dicer_dsRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20930">
        <property type="entry name" value="Dicer_PBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20931">
        <property type="entry name" value="Dicer_platform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00358">
        <property type="entry name" value="DSRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51327">
        <property type="entry name" value="DICER_DSRBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50137">
        <property type="entry name" value="DS_RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00517">
        <property type="entry name" value="RNASE_3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="15" id="KW-0347">Helicase</keyword>
    <keyword evidence="14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="20" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0677">Repeat</keyword>
    <keyword evidence="18 23" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="19" id="KW-0943">RNA-mediated gene silencing</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="28">
        <location>
            <begin position="51"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="29">
        <location>
            <begin position="433"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="domain" description="Dicer dsRNA-binding fold" evidence="30">
        <location>
            <begin position="630"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="domain" description="PAZ" evidence="27">
        <location>
            <begin position="895"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="26">
        <location>
            <begin position="1285"/>
            <end position="1373"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="26">
        <location>
            <begin position="1665"/>
            <end position="1823"/>
        </location>
    </feature>
    <feature type="domain" description="DRBM" evidence="25">
        <location>
            <begin position="1848"/>
            <end position="1913"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="24">
        <location>
            <begin position="409"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="24">
        <location>
            <begin position="414"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00024841"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00035116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS51327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005031559.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1921" mass="217707" checksum="D97F683F2F18EB74" modified="2023-09-13" version="2">MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGAFSRSGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVSASWTKEKWNQEFTKHQVLVMTCYVALNVLKNDYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIEPVVDDDDVFPPYVLRPEDGGPRVTVNTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCIRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPKPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFTLSLQMLELITRLHQYIFSHILRLEKPALEFKPADADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYSKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPVDFRYPNLDFGWKKSIDSKSFISTANSSSAENDSYCKHSTIVVPEHAAHPGANRAPSPEHRDQMSVSCRTLLSRPPGELPTEVSADLAAVNGLSYNGNLANGSYDLANRDFCQGNQLNDYKQEIPVQPTTSSPIQSLYNYENQPKPSDECTLLSNKYLDGNANTSTSDGSPLMAATPGTAEAVKALKARADSEQSPSPGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGRLDRGLEEEEEEDLLWRAPREDADDDDDFLEYDQEHIKFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKGLCPPRENFSSQQKNLSGGWAAASAASSRSSVCKDLEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLEMVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PIX1</accession>
    <name>A0A8C4PIX1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glycoprotein endo-alpha-1,2-mannosidase</fullName>
            <ecNumber evidence="9">3.2.1.130</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">MANEA</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-{alpha-Glc-(1-&gt;3)-alpha-Man-(1-&gt;2)-alpha-Man-(1-&gt;2)-alpha-Man-(1-&gt;3)-[alpha-Man-(1-&gt;2)-alpha-Man-(1-&gt;3)-[alpha-Man-(1-&gt;2)-alpha-Man-(1-&gt;6)]-alpha-Man-(1-&gt;6)]-beta-Man-(1-&gt;4)-beta-GlcNAc-(1-&gt;4)-beta-GlcNAc}-L-asparaginyl-[protein] + H2O = alpha-D-glucosyl-(1-&gt;3)-D-mannopyranose + N(4)-{alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlaNAc-(1-&gt;4)-beta-D-GlcNAc}-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,2)</text>
            <dbReference type="Rhea" id="RHEA:54824"/>
            <dbReference type="Rhea" id="RHEA-COMP:14010"/>
            <dbReference type="Rhea" id="RHEA-COMP:14011"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:52996"/>
            <dbReference type="ChEBI" id="CHEBI:59080"/>
            <dbReference type="ChEBI" id="CHEBI:60627"/>
            <dbReference type="EC" id="3.2.1.130"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 99 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.130" evidence="9"/>
    <dbReference type="RefSeq" id="XP_014698728.1">
        <property type="nucleotide sequence ID" value="XM_014843242.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044613142.1">
        <property type="nucleotide sequence ID" value="XM_044757207.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PIX1"/>
    <dbReference type="Ensembl" id="ENSEAST00005009164.2">
        <property type="protein sequence ID" value="ENSEASP00005008410.1"/>
        <property type="gene ID" value="ENSEASG00005006055.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106832462"/>
    <dbReference type="KEGG" id="eai:106832462"/>
    <dbReference type="CTD" id="79694"/>
    <dbReference type="GeneTree" id="ENSGT00390000016054"/>
    <dbReference type="OMA" id="GFLDYNP"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004569">
        <property type="term" value="F:glycoprotein endo-alpha-1,2-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd11574">
        <property type="entry name" value="GH99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000019">
        <property type="entry name" value="glycoprotein endo-alpha-1,2-mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026071">
        <property type="entry name" value="Glyco_Hydrolase_99"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13572">
        <property type="entry name" value="ENDO-ALPHA-1,2-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13572:SF1">
        <property type="entry name" value="GLYCOPROTEIN ENDO-ALPHA-1,2-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16317">
        <property type="entry name" value="Glyco_hydro_99"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="461" mass="53467" checksum="EAA3F0D3DE6E595B" modified="2022-01-19" version="1">MAKFRRKTCIILSLFILFIFSLMMGLKMLRPNKATFGDPFGLDLLPELRQQSHLGKSFDSQKSDKINSETNVKNLKSVEITIKASKASEPNLEEQPPLNYYLHVFYYSWYGNPQFDGKYVHWNHPILKHWDPKITKNYPQGKHNPPDDIGSSFYPELGSYSSRDPSVIETHMKQMYSASIGVLALSWYPPHSKDENGEPTDDLVPGILDKAHKYNLKVTFHIEPYSNRDENNMYRNVKYIIDKYGNHPAFYRYKTKNGNALPMFYVYDSYITKPEKWANLLTASGSQSIRNSPYDGLFIALLVENKHKYDILRGGFDGIYTYFATNGFTYGSSYENWGRLKFFCDQFNLMFIPSVGPGYIDTSIRPWNAQNTRNRVNGKYYETALSAALQAQPSVISITSFNEWHEGTQIEKAVPKRTSNTVYLDYRPHKPSLYLELTRKWSEKYSKERATYELDHQLPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PKL2</accession>
    <name>A0A8C4PKL2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="16">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">USP1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes.</text>
    </comment>
    <comment type="function">
        <text evidence="14">Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with FANCD2 and PCNA. Interacts with WDR48. Interacts with ATAD5; the interaction regulates USP1-mediated PCNA deubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="16"/>
    <dbReference type="RefSeq" id="XP_044605136.1">
        <property type="nucleotide sequence ID" value="XM_044749201.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PKL2"/>
    <dbReference type="SMR" id="A0A8C4PKL2"/>
    <dbReference type="Ensembl" id="ENSEAST00005013705.2">
        <property type="protein sequence ID" value="ENSEASP00005012616.2"/>
        <property type="gene ID" value="ENSEASG00005008805.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106839757"/>
    <dbReference type="CTD" id="7398"/>
    <dbReference type="GeneTree" id="ENSGT00910000144243"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02671">
        <property type="entry name" value="Peptidase_C19O"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000053">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000077">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033815">
        <property type="entry name" value="USP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF905">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5 16" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 16" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="11" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="7 16" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="18">
        <location>
            <begin position="80"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="228"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="361"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="685"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="228"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="285"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="365"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="708"/>
            <end position="717"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005012616.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PKN6</accession>
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        <submittedName>
            <fullName evidence="13">Zinc finger protein 577</fullName>
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        <name evidence="13" type="primary">ZNF577</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PKN6"/>
    <dbReference type="Ensembl" id="ENSEAST00005013267.2">
        <property type="protein sequence ID" value="ENSEASP00005012212.2"/>
        <property type="gene ID" value="ENSEASG00005008529.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163379"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0001817">
        <property type="term" value="P:regulation of cytokine production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002682">
        <property type="term" value="P:regulation of immune system process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000384">
        <property type="entry name" value="Zinc finger protein 550"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001157">
        <property type="entry name" value="Zinc finger protein 793"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF49">
        <property type="entry name" value="ZINC FINGER PROTEIN 674"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="12">
        <location>
            <begin position="8"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="205"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="261"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="289"/>
            <end position="316"/>
        </location>
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    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="317"/>
            <end position="344"/>
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        <location>
            <begin position="345"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="373"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="11">
        <location>
            <begin position="401"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
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            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
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            <dbReference type="Ensembl" id="ENSEASP00005012212.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="531" mass="60420" checksum="347330FF1D57C8C7" modified="2023-09-13" version="2">MTKAQGALSFEDVAVGFTWEEWQLLDPSQKDLYRDVMLENYNNLLSVGYQAMKPDSLFRLEQGEPPWIAEGAAHSQTCPEEIWEIDHTQWHPENQNEIKTLERCQQSYALANNFDVCKSLVPLTQRHNKFGSHCKSLKSHLGFVIQHRRYAGKDSDEFNGYGKSSLYIQHDKTLTGIKYHGCVKPSSTKSQLSDHQKTYPGEKPHECSECGKAFSRKAQLVRHQRTERGEKPHGCDECGKTFMRKIQLTEHQRTHTGEKPHECNECGKAFSRKSQLMVHQRTHTGEKPYGCSECGKAFSRKCRLNRHQRSHTGEKLYGCSVCGKAFSQKAYLIAHQRLHTGEKPYECSECGRTFFFKSDLTKHQRIHTGEKPYECSECEKAFRSKSKLIQHQRTHTGERPYACSECGKAFAHMSVLIKHKKTHTREKAINPLKMEKAGSGSRRSLYMNELAQEQNAMNTVPVEMSSSGTQTLLNLSELLGGRNVVIVEQPFPRSQPSVDNQEFAQGISLANAVNVTTPSVINYVLYVTDIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PLM3</accession>
    <name>A0A8C4PLM3_EQUAS</name>
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        <recommendedName>
            <fullName evidence="15">Sarcosine dehydrogenase, mitochondrial</fullName>
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    <gene>
        <name evidence="20" type="primary">SARDH</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
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                <person name="Liu"/>
                <person name="G."/>
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                <person name="Guo"/>
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                <person name="Pan"/>
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                <person name="G."/>
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                <person name="R."/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
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                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the last step of the oxidative degradation of choline to glycine. Converts sarcosine into glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(6S)-5,6,7,8-tetrahydrofolyl-(gamma-L-Glu)(n) + sarcosine + oxidized [electron-transfer flavoprotein] + H(+) = (6R)-5,10-methylenetetrahydrofolyl-(gamma-L-Glu)(n) + reduced [electron-transfer flavoprotein] + glycine</text>
            <dbReference type="Rhea" id="RHEA:19793"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="Rhea" id="RHEA-COMP:13257"/>
            <dbReference type="Rhea" id="RHEA-COMP:14738"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57433"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="ChEBI" id="CHEBI:136572"/>
            <dbReference type="ChEBI" id="CHEBI:141005"/>
            <dbReference type="EC" id="1.5.8.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:19794"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13">Amine and polyamine degradation; sarcosine degradation; formaldehyde and glycine from sarcosine: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GcvT family.</text>
    </comment>
    <dbReference type="EC" id="1.5.8.3" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4PLM3"/>
    <dbReference type="Ensembl" id="ENSEAST00005015497.2">
        <property type="protein sequence ID" value="ENSEASP00005014256.2"/>
        <property type="gene ID" value="ENSEASG00005009944.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157589"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008480">
        <property type="term" value="F:sarcosine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.120:FF:000023">
        <property type="entry name" value="Sarcosine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.9.10:FF:000046">
        <property type="entry name" value="Sarcosine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000769">
        <property type="entry name" value="Sarcosine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.110:FF:000006">
        <property type="entry name" value="Sarcosine dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1400:FF:000004">
        <property type="entry name" value="Sarcosine dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.110">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1400">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032503">
        <property type="entry name" value="FAO_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028896">
        <property type="entry name" value="GcvT/YgfZ/DmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029043">
        <property type="entry name" value="GcvT/YgfZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013977">
        <property type="entry name" value="GcvT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCVT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757:SF11">
        <property type="entry name" value="SARCOSINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16350">
        <property type="entry name" value="FAO_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08669">
        <property type="entry name" value="GCV_T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101790">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="16">
        <location>
            <begin position="72"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="FAD dependent oxidoreductase central" evidence="19">
        <location>
            <begin position="433"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="domain" description="GCVT N-terminal" evidence="17">
        <location>
            <begin position="491"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="domain" description="Aminomethyltransferase C-terminal" evidence="18">
        <location>
            <begin position="859"/>
            <end position="933"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00059128"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060644"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00074437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF08669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF16350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014256.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="944" mass="103614" checksum="328C9517FFD120D0" modified="2023-09-13" version="2">MLMPGVPMASLSRALCVAATRPRWSPACSLGLRPLTSGAVPTTEKSVPYQRTLKQAQVAQGPTRPLPSTANVVVIGGGSLGCQTLYHLAKLGVSGAVLLERERLTSGTTWHTAGLLWQLRPSDVEVELLAHTRRVVSRDLEEETGLHTGWIQNGGLFIASNRQRLDEYKRLMSLGKAYGVESHVLSPAETKALYPLMNVDDLYGTLYVPQDGTMDPAGTCTTLARAATARGAQVIENCPVTGIRVRTDDFGVRRVAAVETEHGSIQTPCVVNCAGVWAGAVGRMAGVKVPLVAMHHAYVVTERIEGIQNMPNVRDHDASVYLRLQGDALSVGGYEANPIFWEEVSDKFAFGLFDLDWDVFTQHIEGAINRVPVLEKTGVKSTVCGPESFTPDHKPLMGEAPELRGFFLGCGFNSAGMMLGGGCGQELAHWIVHGRPEKDMYGYDIRRFHHSLTDHRRWIRERSHESYAKNYSVVFPHDEPLAGRNMRRDPLHEELLARGCVFQERHGWERPGWFNPGGGTAPVLPYDYYGAYGHAAHEDHAYGRLLQDEYTFDFPPHHDVIKKECLACRGAAAVFNMSYFGKFYLVGLDARRAADWLFSADVSRPPGSTVYTCMLNQRGGTESDLTVSRLAPGTQASALAPAFEGDGYYLAVGGAAAQHNWSHISTVLQDHKFRCQLVDSSEDLGMISIQGPASRAILQEVLDADLSNEAFPFSTHKLVRAAGHLVRAMRLSFVGELGWELHIPGPSCVPVYQAVMAAGAKHGLVNAGYRAIDSLSIEKGYRHWHSDLRQDDSPLEAGLAFTCKLKSAIPFLGREALERQRAEGLRRRLVCFTVDEPKHRCFPSGASGVPAISVASSRKVPMFGLEAIWRNGQVVGHIRRADFGFAIDKTLAYGYIRDPSGGPVSLDFVKSGDYVLERMGVTYPARAHLKSPFDPDNKRVKGLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PMY8</accession>
    <name>A0A8C4PMY8_EQUAS</name>
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        <submittedName>
            <fullName evidence="4">NYN domain and retroviral integrase containing</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">NYNRIN</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSEAST00005019234.2">
        <property type="protein sequence ID" value="ENSEASP00005017713.1"/>
        <property type="gene ID" value="ENSEASG00005012250.2"/>
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    <dbReference type="GeneTree" id="ENSGT00940000161519"/>
    <dbReference type="OMA" id="PYTHMAV"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036464">
        <property type="term" value="C:cytoplasmic ribonucleoprotein granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09032">
        <property type="entry name" value="KH-I_N4BP1_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22477">
        <property type="entry name" value="KH-I_NYNRIN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18728">
        <property type="entry name" value="PIN_N4BP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11980:FF:000001">
        <property type="entry name" value="ZC3H12A isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11980">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041588">
        <property type="entry name" value="Integrase_H2C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056629">
        <property type="entry name" value="KH_N4BP1_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056630">
        <property type="entry name" value="KH_N4BP1_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021869">
        <property type="entry name" value="RNase_Zc3h12_NYN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002156">
        <property type="entry name" value="RNaseH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041577">
        <property type="entry name" value="RT_RNaseH_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051101">
        <property type="entry name" value="ZC3H12/N4BP1_RNase_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876">
        <property type="entry name" value="N4BP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12876:SF2">
        <property type="entry name" value="PROTEIN NYNRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17921">
        <property type="entry name" value="Integrase_H2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23050">
        <property type="entry name" value="KH_N4BP1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23052">
        <property type="entry name" value="KH_N4BP1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11977">
        <property type="entry name" value="RNase_Zc3h12a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17919">
        <property type="entry name" value="RT_RNaseH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50879">
        <property type="entry name" value="RNASE_H_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-1" evidence="2">
        <location>
            <begin position="1339"/>
            <end position="1485"/>
        </location>
    </feature>
    <feature type="domain" description="Integrase catalytic" evidence="3">
        <location>
            <begin position="1644"/>
            <end position="1765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="475"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="617"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="994"/>
            <end position="1055"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="525"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="666"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="728"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="748"/>
            <end position="757"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1017"/>
            <end position="1040"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005017713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1933" mass="211809" checksum="DC657243D6B77E60" modified="2022-01-19" version="1">MLLSGGDPPAQEWFMVQTKSKPRVQRQRLQVQRIFRVKLNAFQSRPDTPYFWLQLEGPRENMGKAKEYLKGLCSPELWKEVRYPAALHCAFLGAQGLFLDCLCWSTLAYLVPGPPGSLMVGGLTESFIMTQNWLEELVGRLRWGPAPLLTPRGIWEAEVTRAFGALVWIRGDQYAGDLLQLPPAVQELLLSLVRDAAGKEDIIEWLSRIGTSDSRSDPEVLICPPHQQKEGPAMVSVGDGPGPLLEMGTLQNGSPENSKRLTSLGATRSLVSGTPSQNAQQETANQLVRVSSNNHGDTDSAREEGPLQATGSQDSANHTQALSQQRQVQRVEDKLPFQPPVSALGVCPPWKAWAPGPAFGPLWPGAIAATFWRINELHSLHLAWLLSQACLSFPFWQRPLGPIQFKLPGQNALPLNLEWKQKELVPLPSVESPDCRPDGGPGGEAALQNCARPETPKKVMSLLVVSGGSGVKDKVSPGLPQIGPPLTSMPQLQAGSELGDQRSVQLDCKGPEEGPCPVPPTGQEVPPAQGGPVAQGGPTVQSVPDAQTVPETRKVPVAAAVPTAQPPPTNQVLPAAPEVPTAQMMPAVQAEPATPKVPLAPTKPTAQVVPTAQKAAVGQPVSAARMVPAAPKTPTAQKMPAAKASPAGPKTPKAHPGPAAKTGSTAPKAPVAPKAPAAPKAPAAPKAPAAPKASAASKAPRVPQTPAAQKVPTDLGSNLDAAKLLSERQPSSRGSTSFPKGQGDVGRQGSQSSSTLAPRSKHQPQGEGLLGAWEGAPRQPPRHPQANSTVTSFQRYHEALNTPFELNLSGEPGDQGLRRVVIDGSSVAMVHGLQHFFSCRGIAMAVQYFWNRGHREVTVFVPTWQLKKNRRVRESHFLTKLHSLKMLSITPSQLENGKKIATYDYRFMVKLAEETDGIIVTNEQLHILMNNSKKLMVKDRLLPFTFAGNLFMVPDDPLGRDGPTLDEFLKKPNRLDTDIGNFLKVWKTLPPSSASISELSDDADPAPLESPQTVEEVREEEEEVEEEVEEEEGQDEEQREEQEMHEPVEEKDDLDSSLASVFKAECPSLSEEILQCLSLHDPSDRALDIDLLPAVASPHLGIPWDGKPPCQQVLAHLAQLTIPSNFTALSFFVGFMDSHRDVIPDYEALVGPLHSLLKQKPDWQWDREHEKAFLALKRALVSALCLAAPNPQLPFRLEVMVSHVALTAIVYQEHSGRKHPIAYTSKPLLPDEDSEGPQSGGDSPYAVAWALKHFSRCIGDTPVVLDLSYASRTTVDPEAREDRRVSKAWLIRWSLLVQDKGKRALELTLLQGLLGGNRLLTPASSMPRFFQVLPPFSDLSTFVCIHMSGYCFYREDEWCAGFGLYVLSPTSAPVSLSFSCSPYTPTYAHLAAVACGLERFGQSHLPVVFLTHCNWIFSLLWELLPLWKARGFLSSDGAPLPHPSLLSYIISLTSSLSSLPFIYRTSYRGSLFAVTVDTLAKQGAQGGGQWWNLPKDVPAPVVSPHTMGKRPDLLALQLSDSTLADIIAKLQAGQKLSSSSPFSSAFNSLSLDKESGLLMFKGDKKPRVWVVPRQLRRDLIFSVHDLPMGAHQRPEETYKKLRLLGWWPGMQEHVRDYCRSCLFCIPRNLIGSELKVIESLWPLRSTAPWSNLQIEVVGPVTISEEGHKHVLIVADPNTRWVEAFPLKPYTHTAVAQLLLQHVFARWGVPVRLEAAQGPQFARHVLVSCGLALGAQVASLSRDLQFPCLTSSGAYWEFKRALKEFIFLHGKKWAASLPLLHLAFRASSSQATPVQILTGAEVRLTEPLWWEMSSANIEGLKMDVFLLQLMRELLELHCRVADKASEKAENRRFRRESQEKEWNVGDQVLLLSLPRNGSSAKWVGPFYIGDRLSLSLYRVWGFPTPEKLGCIYPSSLMKAFAKSGTPLSFKVLEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PSU9</accession>
    <name>A0A8C4PSU9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Rab GDP dissociation inhibitor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">GDI2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">GDP-dissociation inhibitor preventing the GDP to GTP exchange of most Rab proteins. By keeping these small GTPases in their inactive GDP-bound form regulates intracellular membrane trafficking. Negatively regulates protein transport to the cilium and ciliogenesis through the inhibition of RAB8A.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Regulates the GDP/GTP exchange reaction of most RAB proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with RHOH. Interacts with the GDP-bound inactive forms of RAB3A, RAB3B, RAB3C, RAB5A, RAB5B, RAB5C, RAB8A, RAB8B, RAB10, RAB12, RAB35, and RAB43; binds RAB3D to a lesser extent. Interacts with DZIP1; this interaction negatively regulates the interaction of GDI2 with GDP-bound RAB8A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Golgi apparatus</location>
            <location evidence="3">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the Rab GDI family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044618248.1">
        <property type="nucleotide sequence ID" value="XM_044762313.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PSU9"/>
    <dbReference type="SMR" id="A0A8C4PSU9"/>
    <dbReference type="Ensembl" id="ENSEAST00005030646.2">
        <property type="protein sequence ID" value="ENSEASP00005028219.1"/>
        <property type="gene ID" value="ENSEASG00005019187.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106833790"/>
    <dbReference type="KEGG" id="eai:106833790"/>
    <dbReference type="CTD" id="2665"/>
    <dbReference type="GeneTree" id="ENSGT00950000182994"/>
    <dbReference type="OMA" id="FETKAKM"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005093">
        <property type="term" value="F:Rab GDP-dissociation inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902018">
        <property type="term" value="P:negative regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903565">
        <property type="term" value="P:negative regulation of protein localization to cilium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.405.10:FF:000001">
        <property type="entry name" value="Rab GDP dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.519.10:FF:000005">
        <property type="entry name" value="Rab GDP dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.519.10:FF:000014">
        <property type="entry name" value="Rab GDP dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000158">
        <property type="entry name" value="Rab GDP dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000232">
        <property type="entry name" value="Rab GDP dissociation inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.519.10">
        <property type="entry name" value="Guanine Nucleotide Dissociation Inhibitor, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018203">
        <property type="entry name" value="GDP_dissociation_inhibitor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000806">
        <property type="entry name" value="RabGDI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787:SF1">
        <property type="entry name" value="RAB GDP DISSOCIATION INHIBITOR BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11787">
        <property type="entry name" value="RAB GDP-DISSOCIATION INHIBITOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00996">
        <property type="entry name" value="GDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00892">
        <property type="entry name" value="RABGDI"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00891">
        <property type="entry name" value="RABGDIREP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0007">Acetylation</keyword>
    <keyword evidence="6 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="5 13" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00066224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005028219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="445" mass="50420" checksum="8ABE56413BCFB5BE" modified="2022-01-19" version="1">MNEEYDVIVLGTGLTECILSGIMSVNGKKVLHMDRNPYYGGESASITPLEDLYKRFKIPGAPPASMGRGRDWNVDLIPKFLMANGQLVKMLLYTEVTRYLDFKVTEGSFVYKGGKIYKVPSTEAEALASSLMGLFEKRRFRKFLVYVANFDEKDPRTLEGIDPKKTAMREVYKKFDLGQDVIDFTGHALALYRTDDYLDQPCCETINRIKLYSESLARYGKSPYLYPLYGLGELPQGFARLSAIYGGTYMLNKPIEEIIVQNGKVIGVKSEGEIARCKQLICDPSYVKDRVEKVGQVIRVICILSHPIKNTNDANSCQIIIPQNQVNRKSDIYVCMISSAHNVAAQGKYIAIVSTTVETKEPEKEIRPALELLEPIEQKFVSISDLLVPKDLGTESQIFISRTYDATTHFETTCDDIKDIYKRMTGSEFDFEEMKRKKNDIYGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I817</accession>
    <name>A0A9L0I817_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ankyrin repeat domain 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">ANKRD9</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0I817"/>
    <dbReference type="Ensembl" id="ENSEAST00005043192.1">
        <property type="protein sequence ID" value="ENSEASP00005059413.1"/>
        <property type="gene ID" value="ENSEASG00005036749.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005050973.1">
        <property type="protein sequence ID" value="ENSEASP00005040299.1"/>
        <property type="gene ID" value="ENSEASG00005036749.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005071306.1">
        <property type="protein sequence ID" value="ENSEASP00005036649.1"/>
        <property type="gene ID" value="ENSEASG00005036749.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005071318.1">
        <property type="protein sequence ID" value="ENSEASP00005046668.1"/>
        <property type="gene ID" value="ENSEASG00005036749.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018116"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="170"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005036649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="40709" checksum="7B25A558D1AD0695" modified="2023-09-13" version="1">MPWDARRPGGGARTAGPEGAGAARSRAQKQCRKSSFAFYQAVRDLLPVWLLEDMRASEAFHWDERGRAAAYSPSEALLYALVHDHQAYAHYLLATFPRRALAPPSAGFRCCAAPGPHVALAVRYNRVGILRRILRTVRDFRPRRALARVLDRRRLQPRGGRRHVAARGLRAGAPRVPLPAARPRRLARPARRRRPHAARAAAAPAGGRDAGPPPPPPGRPRSRAPAPPAAARPAGAVHARGRRRPGPPRAAGRPAALAAAAGRGQVPVAGGPGATLALRARHAGAGHRHLSRPLPRGPGRAAAAALPAAAGPHGQGLDRGDTLGAGFLENTIFQSVVPGTLHILISARRNLPRLSPALGAAPGRAGGPHAAGFLGPAALSAPPPCAERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IDY6</accession>
    <name>A0A9L0IDY6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Beta-1,3-N-acetylglucosaminyltransferase</fullName>
            <ecNumber evidence="18">2.4.1.222</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">MFNG</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16 18">
            <text>3-O-(alpha-L-fucosyl)-L-seryl-[EGF-like domain protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-alpha-L-fucosyl)-L-seryl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70511"/>
            <dbReference type="Rhea" id="RHEA-COMP:17919"/>
            <dbReference type="Rhea" id="RHEA-COMP:17920"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:189632"/>
            <dbReference type="ChEBI" id="CHEBI:189633"/>
            <dbReference type="EC" id="2.4.1.222"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 18">
            <text>3-O-(alpha-L-fucosyl)-L-threonyl-[EGF-like domain protein] + UDP-N-acetyl-alpha-D-glucosamine = 3-O-(N-acetyl-beta-D-glucosaminyl-(1-&gt;3)-alpha-L-fucosyl)-L-threonyl-[EGF-like domain protein] + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:70531"/>
            <dbReference type="Rhea" id="RHEA-COMP:17922"/>
            <dbReference type="Rhea" id="RHEA-COMP:17923"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:189631"/>
            <dbReference type="ChEBI" id="CHEBI:189634"/>
            <dbReference type="EC" id="2.4.1.222"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18 21">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the glycosyltransferase 31 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.222" evidence="18"/>
    <dbReference type="RefSeq" id="XP_014706720.1">
        <property type="nucleotide sequence ID" value="XM_014851234.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IDY6"/>
    <dbReference type="Ensembl" id="ENSEAST00005046262.1">
        <property type="protein sequence ID" value="ENSEASP00005039092.1"/>
        <property type="gene ID" value="ENSEASG00005018841.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837591"/>
    <dbReference type="KEGG" id="eai:106837591"/>
    <dbReference type="CTD" id="4242"/>
    <dbReference type="GeneTree" id="ENSGT00940000159564"/>
    <dbReference type="OrthoDB" id="8959630at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033829">
        <property type="term" value="F:O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007389">
        <property type="term" value="P:pattern specification process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.50:FF:000003">
        <property type="entry name" value="Beta-1,3-N-acetylglucosaminyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017374">
        <property type="entry name" value="Fringe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003378">
        <property type="entry name" value="Fringe-like_glycosylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10811">
        <property type="entry name" value="FRINGE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02434">
        <property type="entry name" value="Fringe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038073">
        <property type="entry name" value="B-acetylgalactosaminyltfrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 18" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11 18" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="15 18 21" id="KW-0464">Manganese</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 18 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 18" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Fringe-like glycosyltransferase" evidence="22">
        <location>
            <begin position="50"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="active site" evidence="19">
        <location>
            <position position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="21">
        <location>
            <position position="256"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047713"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049245"/>
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    </evidence>
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        <source>
            <dbReference type="PIRNR" id="PIRNR038073"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02434"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="321" mass="36101" checksum="330B365191ACAB89" modified="2023-09-13" version="1">MQCRLLRGLAGALLTLLCMGLLSLRYHLRLSPQGVQETPEPGPPSSRPPELQLHDVFIAVKTTRAFHRSRLELLLDTWVSRTREQTFIFTDSPDEGLQERLGSHLVVTNCSAEHSHPALSCKMAAEFDAFLASGLRWFCHVDDDNYVNARALLKLLKAFPRTRDIYIGRPSLNRPIHASEPRPRNRTRLVQFWFATGGAGFCINRKLALKMAPWASGPRFVDTSALIRLPDDCTVGYIVECKLGGRLQPSPLFHSHLETLQLLGAAQLPEQVTLSYGVFEGKLNVIKLQGPFSPEEDPSRFRSLHCLLYPDTPWCPQLVAR</sequence>
</entry>
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    <name>A0A9L0IJ31_EQUAS</name>
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        </recommendedName>
        <alternativeName>
            <fullName evidence="13">RNA-binding motif protein 3</fullName>
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    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
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                <person name="Yu"/>
                <person name="J."/>
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                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Dendrite</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IJ31"/>
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        <property type="protein sequence ID" value="ENSEASP00005037053.1"/>
        <property type="gene ID" value="ENSEASG00005008251.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153524"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12449">
        <property type="entry name" value="RRM_CIRBP_RBM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000312">
        <property type="entry name" value="RNA-binding protein 3 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050441">
        <property type="entry name" value="RBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034278">
        <property type="entry name" value="RBM3/CIRBP_RRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48034">
        <property type="entry name" value="TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0346">Stress response</keyword>
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        <location>
            <begin position="6"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
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</entry>
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            <fullName evidence="8">Ig-like domain-containing protein</fullName>
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    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Yang"/>
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                <person name="Fan"/>
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                <person name="Li"/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
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                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0ILY0"/>
    <dbReference type="Ensembl" id="ENSEAST00005082596.1">
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    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162848"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
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    <dbReference type="GO" id="GO:0043296">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005886">
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007156">
        <property type="term" value="P:homophilic cell adhesion via plasma membrane adhesion molecules"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002891">
        <property type="term" value="P:positive regulation of immunoglobulin mediated immune response"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033005">
        <property type="term" value="P:positive regulation of mast cell activation"/>
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        <location>
            <begin position="458"/>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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                <consortium name="Ensembl"/>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038850.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="105" mass="11872" checksum="06918C553B1ECAD4" modified="2023-09-13" version="1">MICFWRSGARAPASCLRRSYVNEGAGHGTRDADGQGKRESGRCPRRPVPEPSVRGGEERRGCRRELDSWRHRLMHCVGFESILEGLYGPRLRRDLSLFEGLSTIS</sequence>
</entry>
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    <accession>A0A9L0ISH8</accession>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="Jordana"/>
                <person name="J."/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="11">Interacts with YBX2.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoplasmic ribonucleoprotein granule</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ZAR1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0ISH8"/>
    <dbReference type="Ensembl" id="ENSEAST00005071294.1">
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        <property type="component" value="Chromosome 3"/>
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    <dbReference type="GO" id="GO:0036464">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048477">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026775">
        <property type="entry name" value="Zar1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027377">
        <property type="entry name" value="ZAR1/RTP1-5-like_Znf-3CxxC"/>
    </dbReference>
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        <property type="entry name" value="ZYGOTE ARREST PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31054">
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    <dbReference type="Pfam" id="PF13695">
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        <property type="entry name" value="zf-3CxxC"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0221">Differentiation</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0896">Oogenesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
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            <begin position="307"/>
            <end position="392"/>
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            <begin position="93"/>
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            <begin position="46"/>
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            <begin position="170"/>
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            <begin position="220"/>
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            <begin position="237"/>
            <end position="246"/>
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            <dbReference type="ARBA" id="ARBA00022473"/>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0ISP2</accession>
    <name>A0A9L0ISP2_EQUAS</name>
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        <recommendedName>
            <fullName evidence="3">thioredoxin-disulfide reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TXNRD3</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
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        <property type="gene ID" value="ENSEASG00005006188.2"/>
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        <property type="component" value="Chromosome 21"/>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:flavin adenine dinucleotide binding"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
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        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
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        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011899">
        <property type="entry name" value="Glutaredoxin_euk/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046952">
        <property type="entry name" value="GSHR/TRXR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006338">
        <property type="entry name" value="Thioredoxin/glutathione_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02180">
        <property type="entry name" value="GRX_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01438">
        <property type="entry name" value="TGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737:SF6">
        <property type="entry name" value="THIOREDOXIN-DISULFIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 11" id="KW-0274">FAD</keyword>
    <keyword evidence="4 11" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="8 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10 11" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0712">Selenocysteine</keyword>
    <feature type="domain" description="Glutaredoxin" evidence="13">
        <location>
            <begin position="77"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="15">
        <location>
            <begin position="166"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="14">
        <location>
            <begin position="521"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005043133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Eutheria</taxon>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">G-protein-coupled receptor of lysophosphatidylserine (LysoPS) that plays different roles in immune response. Acts a damage-sensing receptor that triggers tissue repair upon recognition of dying neutrophils. Mechanistically, apoptotic neutrophils release lysophosphatydilserine that are recognized by type 3 innate lymphoid cells (ILC3s) via GPR34, which activates downstream PI3K-AKT and RAS-ERK signaling pathways leading to STAT3 activation and IL-22 production. Plays an important role in microglial function, controlling morphology and phagocytosis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014840766.3"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:G protein-coupled purinergic nucleotide receptor activity"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="probable G-protein coupled receptor 34"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048057">
        <property type="entry name" value="GPR34_7tmA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24233:SF1">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTOR 34-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24233">
        <property type="entry name" value="P2Y PURINOCEPTOR-RELATED G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00001">
        <property type="entry name" value="7tm_1"/>
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    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01157">
        <property type="entry name" value="P2YPURNOCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
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    <keyword evidence="10 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 13 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 14" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="60"/>
            <end position="80"/>
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            <begin position="92"/>
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    <feature type="transmembrane region" description="Helical" evidence="14">
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            <begin position="311"/>
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    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="71"/>
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    </feature>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
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        </source>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
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            <dbReference type="Proteomes" id="UP000694387"/>
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    <accession>A0A9L0IUX8</accession>
    <name>A0A9L0IUX8_EQUAS</name>
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        <submittedName>
            <fullName evidence="3">Thymocyte expressed, positive selection associated 1</fullName>
        </submittedName>
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        <name evidence="3" type="primary">TESPA1</name>
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            <taxon>Mammalia</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <subcellularLocation>
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        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR048077">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24233">
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    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    <keyword evidence="3 10 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="20"/>
            <end position="39"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005045183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="319" mass="36626" checksum="9DA6E94B8012811D" modified="2023-09-13" version="1">MTNSSTFCPVYRDLEPFTYFFYLVFLVGIIGSCFAAWAFTQKNTNRRCISIYLVNLLTADVLLTLALPVKIVVDLGVAPWKLRIFHCQVTACLIYINMYLSIIFLAFVSIDRCLQLTHSYKVYRIQEPGFAKMISTVVWLMVLLIMVPNMVIPIKDIEEKPNVGCMEFKKEFGRNWHLLTNFICVAIFLNFSAIILISNCLVIRKLYRHKDNEDYPNVKRALISILLVTAAYIICFVPYHIVRIPYTLSQTEVISDCPTRIALFKAKEATLLLAVSNLCFDPILYYHLSKAFRLKVAETFTSRKETKAQKGKSSCENDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IZ84</accession>
    <name>A0A9L0IZ84_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC123287150</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the C/M/P thioester hydrolase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IZ84"/>
    <dbReference type="Ensembl" id="ENSEAST00005049334.1">
        <property type="protein sequence ID" value="ENSEASP00005043062.1"/>
        <property type="gene ID" value="ENSEASG00005036740.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01010000222336"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047617">
        <property type="term" value="F:fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.2240:FF:000001">
        <property type="entry name" value="acyl-coenzyme A thioesterase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000024">
        <property type="entry name" value="acyl-coenzyme A thioesterase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2240">
        <property type="entry name" value="Acyl-CoA thioester hydrolase/BAAT N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016662">
        <property type="entry name" value="Acyl-CoA_thioEstase_long-chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014940">
        <property type="entry name" value="BAAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006862">
        <property type="entry name" value="Thio_Ohase/aa_AcTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042490">
        <property type="entry name" value="Thio_Ohase/BAAT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10824:SF16">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10824">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08840">
        <property type="entry name" value="BAAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04775">
        <property type="entry name" value="Bile_Hydr_Trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016521">
        <property type="entry name" value="Acyl-CoA_hydro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA thioester hydrolase/bile acid-CoA amino acid N-acetyltransferase" evidence="4">
        <location>
            <begin position="16"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="BAAT/Acyl-CoA thioester hydrolase C-terminal" evidence="5">
        <location>
            <begin position="205"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="327"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="3">
        <location>
            <position position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR016521-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF04775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005043062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="422" mass="45707" checksum="560A3CDACD029D2F" modified="2023-09-13" version="1">MAATVTVEPAGRCLWDEPVRIAVRGLAPGQPVTLRASLRDEKGALFRAHARYPAPTPRASWTWSARPALGGSFAGLEPMGLLWALEPEKPLLRLVKRDVQTPFAVQLEVLDGHEPEAGRLLGRAVHERAFLGPGVRREPVRAGRVRATLFLPPEPGPFPGIVDVFGAGGGLLEYRASLLAGKGFAVMALAYYNYDDLPKSMESLHLEYFEEAVNCLLSHPQVKGPGVGLLGISKGGDLCLSMASFLKGITATVIINGSVANVGGALHYKGETLPPVSSNINRIRVTSEGFADILDVLNSPLEGPDQKSFIPVERAESAFLFLVGQDDHNWKSEFYASEASKRLQVHGKERPQIICYPGAGHYIEPPYFPMCPASMHTLVGRPVIWGGEPRAHAMAQVDAWKQLQAFFHKHLGGVKGTIPAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IZF6</accession>
    <name>A0A9L0IZF6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="49">Acyl-coenzyme A thioesterase 8</fullName>
            <ecNumber evidence="5">3.1.2.1</ecNumber>
            <ecNumber evidence="48">3.1.2.11</ecNumber>
            <ecNumber evidence="14">3.1.2.2</ecNumber>
            <ecNumber evidence="47">3.1.2.27</ecNumber>
            <ecNumber evidence="46">3.1.2.3</ecNumber>
            <ecNumber evidence="45">3.1.2.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="50">Choloyl-coenzyme A thioesterase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="52">Peroxisomal acyl-coenzyme A thioester hydrolase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="51">Peroxisomal long-chain acyl-CoA thioesterase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="55" type="primary">ACOT8</name>
    </gene>
    <organism evidence="55 56">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="55 56" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="55" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="55" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="44">Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels. Displays no strong substrate specificity with respect to the carboxylic acid moiety of Acyl-CoAs. Hydrolyzes medium length (C2 to C20) straight-chain, saturated and unsaturated acyl-CoAS but is inactive towards substrates with longer aliphatic chains. Moreover, it catalyzes the hydrolysis of CoA esters of bile acids, such as choloyl-CoA and chenodeoxycholoyl-CoA and competes with bile acid CoA:amino acid N-acyltransferase (BAAT). Is also able to hydrolyze CoA esters of dicarboxylic acids. It is involved in the metabolic regulation of peroxisome proliferation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>(3S)-3-hydroxy-3-methylglutaryl-CoA + H2O = 3-hydroxy-3-methylglutarate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16305"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17325"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="EC" id="3.1.2.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="29">
            <dbReference type="Rhea" id="RHEA:16306"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40151"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32395"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57368"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:40152"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(9Z)-hexadecenoyl-CoA + H2O = (9Z)-hexadecenoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40131"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32372"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:61540"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:40132"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(9Z)-octadecenoyl-CoA + H2O = (9Z)-octadecenoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40139"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30823"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:40140"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>(9Z)-tetradecenoyl-CoA + H2O = (9Z)-tetradecenoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40135"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32370"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65060"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:40136"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="35">
            <text>(9Z,12Z)-octadecadienoyl-CoA + H2O = (9Z,12Z)-octadecadienoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40143"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30245"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57383"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="35">
            <dbReference type="Rhea" id="RHEA:40144"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="34">
            <text>2,6-dimethylheptanoyl-CoA + H2O = 2,6-dimethylheptanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:59952"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:84847"/>
            <dbReference type="ChEBI" id="CHEBI:143533"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="34">
            <dbReference type="Rhea" id="RHEA:59953"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>2-methyloctadecanoyl-CoA + H2O = 2-methyloctadecanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:59940"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:143530"/>
            <dbReference type="ChEBI" id="CHEBI:143531"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="26">
            <dbReference type="Rhea" id="RHEA:59941"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oyl-CoA + H2O = 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-26-oate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:59936"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:63001"/>
            <dbReference type="ChEBI" id="CHEBI:85674"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="40">
            <dbReference type="Rhea" id="RHEA:59937"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>4,8-dimethylnonanoyl-CoA + H2O = 4,8-dimethylnonanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40223"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:77061"/>
            <dbReference type="ChEBI" id="CHEBI:77063"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:40224"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>acetoacetyl-CoA + H2O = acetoacetate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15673"/>
            <dbReference type="ChEBI" id="CHEBI:13705"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="EC" id="3.1.2.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="21">
            <dbReference type="Rhea" id="RHEA:15674"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="43">
            <text>acetyl-CoA + H2O = acetate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="3.1.2.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="43">
            <dbReference type="Rhea" id="RHEA:20290"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>butanoyl-CoA + H2O = butanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40111"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17968"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57371"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:40112"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>chenodeoxycholoyl-CoA + H2O = chenodeoxycholate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31511"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36234"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:62989"/>
            <dbReference type="EC" id="3.1.2.27"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="27">
            <dbReference type="Rhea" id="RHEA:31512"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="33">
            <text>choloyl-CoA + H2O = cholate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14541"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29747"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57373"/>
            <dbReference type="EC" id="3.1.2.27"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="33">
            <dbReference type="Rhea" id="RHEA:14542"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>decanedioyl-CoA + H2O = decanedioate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40591"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:76283"/>
            <dbReference type="ChEBI" id="CHEBI:76316"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="23">
            <dbReference type="Rhea" id="RHEA:40592"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>decanoyl-CoA + H2O = decanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40059"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:27689"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:61430"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:40060"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>dodecanedioyl-CoA + H2O = dodecanedioate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40595"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:76273"/>
            <dbReference type="ChEBI" id="CHEBI:76315"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="36">
            <dbReference type="Rhea" id="RHEA:40596"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>dodecanoyl-CoA + H2O = dodecanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30135"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18262"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:30136"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="37">
            <text>eicosanoyl-CoA + H2O = eicosanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40147"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32360"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57380"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="37">
            <dbReference type="Rhea" id="RHEA:40148"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="32">
            <text>glutaryl-CoA + H2O = glutarate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40575"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30921"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57378"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="32">
            <dbReference type="Rhea" id="RHEA:40576"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>hexadecanoyl-CoA + H2O = hexadecanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16645"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="EC" id="3.1.2.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:16646"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>hexanedioyl-CoA + H2O = hexanedioate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40583"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17128"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:76327"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="28">
            <dbReference type="Rhea" id="RHEA:40584"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>hexanoyl-CoA + H2O = hexanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40115"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17120"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:62620"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:40116"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>malonyl-CoA + H2O = malonate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40219"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15792"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="38">
            <dbReference type="Rhea" id="RHEA:40220"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="31">
            <text>octadecanoyl-CoA + H2O = octadecanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30139"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25629"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="31">
            <dbReference type="Rhea" id="RHEA:30140"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>octanedioyl-CoA + H2O = octanedioate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40587"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:76282"/>
            <dbReference type="ChEBI" id="CHEBI:76317"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="25">
            <dbReference type="Rhea" id="RHEA:40588"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>octanoyl-CoA + H2O = octanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30143"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:25646"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57386"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:30144"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="41">
            <text>propanoyl-CoA + H2O = propanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40103"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17272"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57392"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="41">
            <dbReference type="Rhea" id="RHEA:40104"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="30">
            <text>prostaglandin F2alpha-CoA + H2O = prostaglandin F2alpha + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:59948"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57404"/>
            <dbReference type="ChEBI" id="CHEBI:143532"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="30">
            <dbReference type="Rhea" id="RHEA:59949"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="42">
            <text>succinyl-CoA + H2O = succinate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11516"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="EC" id="3.1.2.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="42">
            <dbReference type="Rhea" id="RHEA:11517"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>tetradecanoyl-CoA + H2O = tetradecanoate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:40119"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30807"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:40120"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the C/M/P thioester hydrolase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.1" evidence="5"/>
    <dbReference type="EC" id="3.1.2.11" evidence="48"/>
    <dbReference type="EC" id="3.1.2.2" evidence="14"/>
    <dbReference type="EC" id="3.1.2.27" evidence="47"/>
    <dbReference type="EC" id="3.1.2.3" evidence="46"/>
    <dbReference type="EC" id="3.1.2.5" evidence="45"/>
    <dbReference type="RefSeq" id="XP_070341505.1">
        <property type="nucleotide sequence ID" value="XM_070485404.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IZF6"/>
    <dbReference type="Ensembl" id="ENSEAST00005071431.1">
        <property type="protein sequence ID" value="ENSEASP00005042450.1"/>
        <property type="gene ID" value="ENSEASG00005029828.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106843034"/>
    <dbReference type="CTD" id="10005"/>
    <dbReference type="GeneTree" id="ENSGT00390000004207"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005782">
        <property type="term" value="C:peroxisomal matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047603">
        <property type="term" value="F:acetoacetyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003986">
        <property type="term" value="F:acetyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033882">
        <property type="term" value="F:choloyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047994">
        <property type="term" value="F:hydroxymethylglutaryl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052816">
        <property type="term" value="F:long-chain fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052815">
        <property type="term" value="F:medium-chain fatty acyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004778">
        <property type="term" value="F:succinyl-CoA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006637">
        <property type="term" value="P:acyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036109">
        <property type="term" value="P:alpha-linolenic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043649">
        <property type="term" value="P:dicarboxylic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009062">
        <property type="term" value="P:fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901570">
        <property type="term" value="P:fatty acid derivative biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042759">
        <property type="term" value="P:long-chain fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010561">
        <property type="term" value="P:negative regulation of glycoprotein biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006636">
        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd03444">
        <property type="entry name" value="Thioesterase_II_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03445">
        <property type="entry name" value="Thioesterase_II_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.210:FF:000002">
        <property type="entry name" value="acyl-coenzyme A thioesterase 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.210">
        <property type="entry name" value="Acyl-CoA thioesterase, double hotdog domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042171">
        <property type="entry name" value="Acyl-CoA_hotdog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003703">
        <property type="entry name" value="Acyl_CoA_thio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049449">
        <property type="entry name" value="TesB_ACOT8-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025652">
        <property type="entry name" value="TesB_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00189">
        <property type="entry name" value="tesB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066">
        <property type="entry name" value="ACYL-COA THIOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11066:SF34">
        <property type="entry name" value="ACYL-COENZYME A THIOESTERASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13622">
        <property type="entry name" value="4HBT_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02551">
        <property type="entry name" value="Acyl_CoA_thio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
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    </dbReference>
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    <keyword evidence="9" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="7" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="56" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="Acyl-CoA thioesterase-like N-terminal HotDog" evidence="54">
        <location>
            <begin position="44"/>
            <end position="127"/>
        </location>
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            <begin position="198"/>
            <end position="278"/>
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            <dbReference type="ARBA" id="ARBA00023098"/>
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            <dbReference type="ARBA" id="ARBA00047734"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00047969"/>
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</entry>
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    <accession>A0A9L0J0D0</accession>
    <name>A0A9L0J0D0_EQUAS</name>
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        <submittedName>
            <fullName evidence="4">Growth arrest and DNA damage inducible beta</fullName>
        </submittedName>
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    <gene>
        <name evidence="4" type="primary">GADD45B</name>
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    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the GADD45 family.</text>
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        <property type="gene ID" value="ENSEASG00005033645.1"/>
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        <property type="component" value="Chromosome 20"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046330">
        <property type="term" value="P:positive regulation of JNK cascade"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900745">
        <property type="term" value="P:positive regulation of p38MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051726">
        <property type="term" value="P:regulation of cell cycle"/>
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        <property type="project" value="Ensembl"/>
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        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR024824">
        <property type="entry name" value="GADD45"/>
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    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10411">
        <property type="entry name" value="GROWTH ARREST AND DNA DAMAGE-INDUCIBLE PROTEIN GADD45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10411:SF5">
        <property type="entry name" value="GROWTH ARREST AND DNA DAMAGE-INDUCIBLE PROTEIN GADD45 BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="3">
        <location>
            <begin position="22"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="183"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="211"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005043472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="224" mass="23958" checksum="233105D3A02E8C51" modified="2023-09-13" version="1">MTLEELVACDNAAQKMQTVSAAVEELLVAAQRQDRLTVGVYESAKLMNVDPDSVVLCLLAIDEEEEDDIALQIHFTLIQSFCCDNDINIVRVSGMQRLAQLLGEPAETQGTTEARDLHCLLVTVSWASAVLPRPTDPAPGPCLSTKLVSHQLSTQPCLACPVGSRAWASVTQRAILSQPVFPKRALGEGGSPPTPGAPAEPRLLSPPAEPSYRRLEKPRPGGGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J1Y2</accession>
    <name>A0A9L0J1Y2_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Colipase like 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">CLPSL2</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_014686330.1">
        <property type="nucleotide sequence ID" value="XM_014830844.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J1Y2"/>
    <dbReference type="Ensembl" id="ENSEAST00005065027.1">
        <property type="protein sequence ID" value="ENSEASP00005047271.1"/>
        <property type="gene ID" value="ENSEASG00005035770.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106824523"/>
    <dbReference type="CTD" id="389383"/>
    <dbReference type="GeneTree" id="ENSGT00390000011494"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007586">
        <property type="term" value="P:digestion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032094">
        <property type="term" value="P:response to food"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.80.10">
        <property type="entry name" value="Lipase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001981">
        <property type="entry name" value="Colipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10041">
        <property type="entry name" value="COLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10041:SF3">
        <property type="entry name" value="COLIPASE-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51342">
        <property type="entry name" value="COLIPASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040505243" evidence="1">
        <location>
            <begin position="18"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005047271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="100" mass="10517" checksum="2800A6BDD64F9C1B" modified="2023-09-13" version="1">MAAAAAILAGVLLPCWGAFPQLNKGLLKRNGAGCSHHLECLSDCCLMDLDHGGTFCAPKAGRAMTCLPQTKGATNIMCPCRMGLTCVSKDLACPRRCHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J5V7</accession>
    <name>A0A9L0J5V7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dual-specificity kinase</fullName>
            <ecNumber evidence="3">2.7.12.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">DYRK1B</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10596"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.12.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.12.1" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0J5V7"/>
    <dbReference type="Ensembl" id="ENSEAST00005050738.1">
        <property type="protein sequence ID" value="ENSEASP00005045407.1"/>
        <property type="gene ID" value="ENSEASG00005011791.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160345"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004712">
        <property type="term" value="F:protein serine/threonine/tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060612">
        <property type="term" value="P:adipose tissue development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007520">
        <property type="term" value="P:myoblast fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14226">
        <property type="entry name" value="PKc_DYRK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000087">
        <property type="entry name" value="Dual specificity tyrosine-phosphorylation-regulated kinase 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000264">
        <property type="entry name" value="dual specificity tyrosine-phosphorylation-regulated kinase 1B isoform X3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044131">
        <property type="entry name" value="PKc_DYR1A/1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF12">
        <property type="entry name" value="DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION-REGULATED KINASE 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0829">Tyrosine-protein kinase</keyword>
    <feature type="domain" description="Protein kinase" evidence="17">
        <location>
            <begin position="290"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="125"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="246"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="559"/>
            <end position="578"/>
        </location>
    </feature>
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            <begin position="615"/>
            <end position="808"/>
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        <location>
            <begin position="1"/>
            <end position="11"/>
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    <feature type="compositionally biased region" description="Gly residues" evidence="16">
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            <begin position="18"/>
            <end position="30"/>
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            <begin position="160"/>
            <end position="174"/>
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            <begin position="753"/>
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            <position position="319"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:30616"/>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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    <evidence type="ECO:0000256" key="2">
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            <dbReference type="ARBA" id="ARBA00008867"/>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50011"/>
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            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="808" mass="86824" checksum="C33D5AD65556E6A6" modified="2023-09-13" version="1">MAGADCQTQSGPARLGSAGWGGPLGWGAGRRGPRGRVAARGGAGPAGRGLSRPLPRPPAFSFPRVPRADPRPLLSPPPAAAAAVVTHRAACPACPPRRGACRAGRAGTGGRLRTAATVAMLAARPPHWGPHRAPAPRGPRASPDPGLSGGGSRGAGCEKAPPGRAPAPGLAPLRPSEPTMAVPPGHGPFSGFPGPQEHTQVLPDVRLLPRRLPLAFRDATSAPLRKLSVDLIKTYKHINEVYYAKKKRRAQQAPPQDSSTKKEKKVLNHGYDDDNHDYIVRSGERWLERYEIDSLIGKGSFGQVVKAYDHQTQELVAIKIIKNKKAFLNQAQIELRLLELMNRHDTEMKYYIVHLKRHFMFRNHLCLVFELLSYNLYDLLRNTHFRGVSLNLTRKLAQQLCTALLFLATPELSIIHCDLKPENILLCNPKRSAIKIVDFGSSCQLGQRIYQYIQSRFYRSPEVLLGTPYDLAIDMWSLGCILVEMHTGEPLFSGSNEVDQMSRIVEVLGIPPAPMLDQAPKARKYFERLPGGGWTLRRTKELRKDYQGPGTRRLQEVLGVQTGGPGGRRAGEPGHSPADYLRFQDLVLRMLEYEPAARISPLGALQHGFFRRTADEATNTGPAGSSASTSPAPLDTCPSSSTASSISSSGGSSGSSSDNRTYRYSNRYCGGPGPPITDCEMNSPQVPASQPLRPWAGGDVPHKTHQAPASASSLPGAGAQLPPQPRCLGRPPSPTSPPPPELMDVSLVGGPPDCSPPHPAPAPQHPAASALRTRMTGGRPPLPPPDDPATLGPRLGLRGVPQSTAASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J8X1</accession>
    <name>A0A9L0J8X1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Zyxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">ZYX</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Adhesion plaque protein. Binds alpha-actinin and the CRP protein. Important for targeting TES and ENA/VASP family members to focal adhesions and for the formation of actin-rich structures. May be a component of a signal transduction pathway that mediates adhesion-stimulated changes in gene expression.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell junction</location>
            <location evidence="3">Focal adhesion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the zyxin/ajuba family.</text>
    </comment>
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        <property type="gene ID" value="ENSEASG00005009228.2"/>
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        <property type="component" value="Chromosome 1"/>
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        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="C:focal adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="C:phagocytic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001725">
        <property type="term" value="C:stress fiber"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007160">
        <property type="term" value="P:cell-matrix adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071346">
        <property type="term" value="P:cellular response to type II interferon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007229">
        <property type="term" value="P:integrin-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043149">
        <property type="term" value="P:stress fiber assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd09353">
        <property type="entry name" value="LIM2_Zyxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000027">
        <property type="entry name" value="lipoma-preferred partner isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000057">
        <property type="entry name" value="Zyxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000076">
        <property type="entry name" value="Zyxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24212:SF1">
        <property type="entry name" value="ZYXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24212">
        <property type="entry name" value="ZYXIN/TRIP6"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00412">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
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        <property type="match status" value="3"/>
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    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
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    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="10" id="KW-0965">Cell junction</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11 16" id="KW-0440">LIM domain</keyword>
    <keyword evidence="6 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="18">
        <location>
            <begin position="381"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="18">
        <location>
            <begin position="441"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
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            <begin position="23"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
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            <begin position="156"/>
            <end position="350"/>
        </location>
    </feature>
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            <begin position="63"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
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            <begin position="93"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
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            <begin position="202"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="253"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="318"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004246"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009611"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039396"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005049609.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="601" mass="64534" checksum="B692EC5FEE386DA4" modified="2023-09-13" version="1">MAAPRPPPAISVSVSAPAFYAPQKKFGPVVAPKPKVNPFRPGDSEAPSAAGAQRAQMGRVGEIPPPPPEDLPLPPPPLLGDGDDTEGALGGAFPPPPPPIEEPFPPAPLEEEIFPSPPPPVVEEGGPEAPIPLPPQPREKVSSIDLEIDSLSSLLDDMTKNDPFKARVSSGYTPPAVATPFISKSSTKPATGGTAPLPPWKSPSSSQSLPQVQAQSPSQTQFHVQPQPQAKPQVQLHVQPQPQSVPLANTQPRGPPAPPPAPKFSPVTPKFTPVASKFSPGAPGGPGSQPNQKLGPPEVPSSTGPGSPQPPSFTYAQQREKPRVQEKQHPVPPPAQNQNQVRSPGAPGPLTLKEVEELEQLTQQLMQDMEHPQRQNVAVNEFCGRCHQPLARAQPAVRALGQLFHITCFTCHQCEQQLQGQQFYSLEGAPYCEGCYTDTLEKCNTCGQPITDRMLRATGKAYHPQCFTCVVCACPLEGTSFIVDQNNRPHCVPDYHKQYAPRCSVCAEPIMPEPGREETVRVVALDKNFHMKCYKCEVSCPCGPSGLEWWDTAHQPGGGWRQPCGNGCCNFEGGQWQCPCPRAQMPPLLQERECGGFGGLL</sequence>
</entry>
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        <recommendedName>
            <fullName evidence="2">BMERB domain-containing protein</fullName>
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        <name evidence="3" type="primary">BMERB1</name>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
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            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        </source>
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            <dbReference type="PROSITE" id="PS51848"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
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    <accession>A0A9L0JCD5</accession>
    <name>A0A9L0JCD5_EQUAS</name>
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        <submittedName>
            <fullName evidence="4">S-phase cyclin A associated protein in the ER</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">SCAPER</name>
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    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
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    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Yin"/>
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                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_014696917.1">
        <property type="nucleotide sequence ID" value="XM_014841431.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014696918.1">
        <property type="nucleotide sequence ID" value="XM_014841432.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044620105.1">
        <property type="nucleotide sequence ID" value="XM_044764170.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005060443.1">
        <property type="protein sequence ID" value="ENSEASP00005050966.1"/>
        <property type="gene ID" value="ENSEASG00005015656.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831301"/>
    <dbReference type="KEGG" id="eai:106831301"/>
    <dbReference type="CTD" id="49855"/>
    <dbReference type="GeneTree" id="ENSGT00390000011159"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001547">
        <property type="term" value="P:antral ovarian follicle growth"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060041">
        <property type="term" value="P:retina development in camera-type eye"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000680">
        <property type="entry name" value="S phase cyclin A-associated protein in the endoplasmic reticulum"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032446">
        <property type="entry name" value="SCAPER_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31434">
        <property type="entry name" value="S PHASE CYCLIN A-ASSOCIATED PROTEIN IN THE ENDOPLASMIC RETICULUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31434:SF2">
        <property type="entry name" value="S PHASE CYCLIN A-ASSOCIATED PROTEIN IN THE ENDOPLASMIC RETICULUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16501">
        <property type="entry name" value="SCAPER_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="U1-type" evidence="3">
        <location>
            <begin position="797"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="37"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="426"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="547"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="875"/>
            <end position="886"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
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    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        </source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1408" mass="158876" checksum="DDF43815FB93C786" modified="2023-09-13" version="1">MMASFQRSNSHDKVRRIVAEEGRTARNLIAWSVPLESKDDDGKSKCQAGGKSKRTIQSTHKTTKQNTAVDCKITSSTAGDKHFDKSPTKTRHPRKIDLRARYWAFLFDNLRRAVDEIYVTCESDQSVVECKEVLMMLDNYVRDFKALIDWIQLQEKLEKTDAQSRPTSLAWEVKKMSPGRHVIPSPSTDRISVTSNARRSLNFGSSPGTGAAPRLAPTGVSWADKVKAHHTGSTASLDVAPAQSCPPMPVQKTSRKNERKDAEGWETVQRGRPVRSRSTAVMPKLSLATEALRSKDDSDKENVRLLPDESIQKGEFVRDGPSNTVESRPKDSLQFCDHPLAERSQFTVSTLDDVKNSGSSFLQDSSVRTSEIPAVYIDTECVSITQQADTPPLQTNEDRFSAEKARIESEMDPSDISNVSAANLSMAEVLAKKEELADRLEKANEEAIASAIAEEEQLTREIEAEENNDINIETDNDSDFSASVGSGSVSFCGMSMDWNDVLADYEARESWRQNTSWGDIVEEEPARPPGHGIHMHEKLSSPSRKRTIAESKKKHEEKQMKAQQLREKLREEKTLKLQKLLEREKDVRKWKEELLDQRRRMMEEKLLHAEFKREVQLQAIVKKAQEEEAKVNEIAFINTLEAQNKRHDVLSKLKEYEQRLNELQEERQRRQEEKQARDEAVQERKRALEAERQARVEELLMKRKEQEARIEQQRQEKEKAREDAARERARDREERLAALTAAQQEAMEELQKKIQLKHDESIRRHMEQIEQRKEKAAELSSGRHANTDYAPKLTPYERKKQCSLCNVLISSEVYLFSHIKGKKHQQAVRENSSIQGRELSDEEVEHLSLKKYIIDIVVESAAPAEPLKDGEERQKNKKKAKKIKARMNSRAKEYESLMETKNSGSDSPYKAKLQRLAKDLLKQQQVQDSGSWANNKVSALDRTLGEIARILEKENVADQIAFQVAGGLTALEHILQVVIPAANLNTVSRIPPKSLCNAVNVYNLTCNNCSENCTDVLFSNKITFLMDLLIHQLTVYVPDEKNAILGRNTNKQVFEGLTTGLLKVSAVVFGCLIASRPDGNSQLATSKISTQEMKNKPSQGDAFNSRVQDLISYVVNIGLIEKLCGCFLSVQGPVDENPKMATFLQHAAGLLDGMCTLCFAVTRRSYSIFDNNRQDPTGLTAALQATDMAGVLHMLYCVLFHGTVSDPSTASPKESYAQNTIQVAVQSLRFFNSFAALDLPAFQSIVGAEGLSLAFRHIASSLLGHCSQVSCESLLHEIIVCVGYFTVNHPDNQVIVQSGRHPTVLQKLCQLPFQYFSDPRLIKVLFPSLIAACYNNHQNKIILEQEMSCVLLATFIQDFAQTPGQADNQSDQSKGKCLGSQDYLELANRFPRQAWEEARQFFLKKEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JCT6</accession>
    <name>A0A9L0JCT6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Mitochondrial import inner membrane translocase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">TIMM9</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Mitochondrial intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. May also be required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="function">
        <text evidence="15">Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Heterohexamer; composed of 3 copies of TIMM9 and 3 copies of TIMM10/TIM10A, named soluble 70 kDa complex. The complex forms a 6-bladed alpha-propeller structure and associates with the TIMM22 component of the TIM22 complex. Interacts with multi-pass transmembrane proteins in transit. Also forms a complex composed of TIMM9, TIMM10/TIM10A and FXC1/TIM10B.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Mitochondrion inner membrane</location>
            <topology evidence="1 15">Peripheral membrane protein</topology>
            <orientation evidence="1 15">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="15">The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the small Tim family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014719785.1">
        <property type="nucleotide sequence ID" value="XM_014864299.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014719786.1">
        <property type="nucleotide sequence ID" value="XM_014864300.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JCT6"/>
    <dbReference type="SMR" id="A0A9L0JCT6"/>
    <dbReference type="Ensembl" id="ENSEAST00005062091.1">
        <property type="protein sequence ID" value="ENSEASP00005050168.1"/>
        <property type="gene ID" value="ENSEASG00005032848.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106845857"/>
    <dbReference type="KEGG" id="eai:106845857"/>
    <dbReference type="CTD" id="26520"/>
    <dbReference type="GeneTree" id="ENSGT00940000160102"/>
    <dbReference type="OMA" id="QDFLRMY"/>
    <dbReference type="OrthoDB" id="1551503at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042719">
        <property type="term" value="C:mitochondrial intermembrane space chaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032977">
        <property type="term" value="F:membrane insertase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.810:FF:000004">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit Tim9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.810">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit tim13 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050673">
        <property type="entry name" value="Mito_inner_translocase_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004217">
        <property type="entry name" value="Tim10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035427">
        <property type="entry name" value="Tim10-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13172">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM9B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02953">
        <property type="entry name" value="zf-Tim10_DDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144122">
        <property type="entry name" value="Tim10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11 15" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 15" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 15" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7 15" id="KW-0653">Protein transport</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 15" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 15" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tim10-like" evidence="16">
        <location>
            <begin position="9"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00063641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF02953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="89" mass="10378" checksum="00F17CF6332ABF48" modified="2023-09-13" version="1">MAAQIPESDQIKQFKEFLGTYNKLTETCFLDCVKDFTTREVKPEETTCSEHCLQKYLKMTQRISMRFQEYHIQQNEALAAKAGLLGQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JDJ7</accession>
    <name>A0A9L0JDJ7_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Proteasome 20S subunit alpha 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">PSMA2</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase T1A family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JDJ7"/>
    <dbReference type="SMR" id="A0A9L0JDJ7"/>
    <dbReference type="Ensembl" id="ENSEAST00005083596.1">
        <property type="protein sequence ID" value="ENSEASP00005048077.1"/>
        <property type="gene ID" value="ENSEASG00005017678.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000074870"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
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                <person name="X. and Zhong"/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="1" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JEQ0"/>
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    </dbReference>
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        <property type="component" value="Chromosome X"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11667:SF27">
        <property type="entry name" value="ADENYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="194" mass="21065" checksum="0508146D1A6AEFE5" modified="2023-09-13" version="1">FSLCHLQLGDVSHRFWTQDVASPVTADLVVSVVVVGPDSFHQLSQSSFVFRVDLCDGDGGTGLPVDQTPQPGLGLDDAVGHPHLPTQGWKEDDQLDGIHVVCDHYQLSLLVLHQGGDSIDPCSKDRWPLSGDIPFASSLLLSPGQQPLLLLLLCLWSVLVGQLEQLSSYLAVQGLGELVDRGRYLDPLIEDRPL</sequence>
</entry>
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    <name>A0A9L0JGZ5_EQUAS</name>
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        <recommendedName>
            <fullName evidence="7">Myelin and lymphocyte protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106838524</name>
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    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the MAL family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JGZ5"/>
    <dbReference type="Ensembl" id="ENSEAST00005078436.1">
        <property type="protein sequence ID" value="ENSEASP00005049287.1"/>
        <property type="gene ID" value="ENSEASG00005033988.1"/>
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        <property type="component" value="Chromosome 6"/>
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        <property type="term" value="C:membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0019911">
        <property type="term" value="F:structural constituent of myelin sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:myelination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="entry name" value="MAL"/>
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        <property type="entry name" value="Marvel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050578">
        <property type="entry name" value="MARVEL-CKLF_proteins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776">
        <property type="entry name" value="MARVEL-CONTAINING POTENTIAL LIPID RAFT-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22776:SF12">
        <property type="entry name" value="MYELIN AND LYMPHOCYTE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01284">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01884">
        <property type="entry name" value="MALPROTEIN"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51225">
        <property type="entry name" value="MARVEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="68"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="MARVEL" evidence="10">
        <location>
            <begin position="31"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005049287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="182" mass="20668" checksum="5E55B232FB7DDB12" modified="2023-09-13" version="1">MPPAVLCLHPVFLPLVPQRDLWVWPFLGVDSPGPMLTLPSFPLQVFGGLVWILVASSLVPLPLTQGWVMFVSVFCFINTTVLFFLYVISGNYEETSWVFLEAAYHCIASVFYLSVSILEGLATIVIHDGFAYEHYLENVFATVFSSIATLLYVEHAWFSLIRWKWFSLRRKKTSSQNPDGVN</sequence>
</entry>
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        <recommendedName>
            <fullName evidence="14">ADP-ribosylation factor</fullName>
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            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
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                <person name="Z."/>
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                <person name="J."/>
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                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="14">GTP-binding protein involved in protein trafficking; modulates vesicle budding and uncoating within the Golgi apparatus.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the small GTPase superfamily. Arf family.</text>
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    </dbReference>
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    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
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    <dbReference type="GO" id="GO:0005794">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04150">
        <property type="entry name" value="Arf1_5_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000024">
        <property type="entry name" value="ADP-ribosylation factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045872">
        <property type="entry name" value="Arf1-5-like"/>
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    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
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    <keyword evidence="7 14" id="KW-0653">Protein transport</keyword>
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    <keyword evidence="3 14" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="24"/>
            <end position="31"/>
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        <ligand>
            <name>GTP</name>
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    <feature type="binding site" evidence="12">
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            <position position="31"/>
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        <ligand>
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            <position position="48"/>
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            <name>GTP</name>
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            <dbReference type="RuleBase" id="RU369003"/>
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            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="180" mass="20530" checksum="25EF3D4895408147" modified="2023-09-13" version="1">MGLTVSALFSRIFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVQESADELQKMLQEDELRDAVLLVFANKQDMPNAMPVSELTDKLGLQHLRSRTWYVQATCATQGTGLYDGLDWLSHELSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JKI4</accession>
    <name>A0A9L0JKI4_EQUAS</name>
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        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
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        <name evidence="15" type="primary">LOC106843940</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
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    </organism>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
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                <person name="J."/>
                <person name="Pan"/>
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                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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    <comment type="subcellular location">
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            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="component" value="Chromosome 20"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="entry name" value="Olfact_rcpt"/>
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        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="7tm_4"/>
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    </dbReference>
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        <property type="entry name" value="GPCRRHODOPSN"/>
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        <property type="entry name" value="OLFACTORYR"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
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    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
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    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
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    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="25"/>
            <end position="51"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="63"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="144"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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            <end position="229"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="241"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="276"/>
            <end position="295"/>
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    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="44"/>
            <end position="293"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022725"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363047"/>
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    </evidence>
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            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="314" mass="34632" checksum="854DADD64560D5C7" modified="2023-09-13" version="1">MDALGDGNHTSTTGFILLGLTNDPILRIILFMIILCIYLVTISGNLSAIILIRISSQLHHPMYFFLSHLALADIGYSSSVTPNMLVNFLVGRNTISYFGCATQLGSVVFFGTAEFFLLAAMAYDRFVAICSPLLYSTKMSTEVCVQLLVVAYIGGFLNACSFTICFYSLVFCGPNRVNHFFCDFAPLVELSCSDISIPAAVPSFTAGSIIVVTVLVIAVSYIYILITILKMRSTEGRHKAFSTCTSHLTVVTLFYGTITYIYVVPKSSYSTDQNKVVSVFYMVVIPMLNPLIYSLRNNEIKGALKRELARKIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JP24</accession>
    <name>A0A9L0JP24_EQUAS</name>
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        <submittedName>
            <fullName evidence="3">Coiled-coil domain containing 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">CCDC14</name>
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    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JP24"/>
    <dbReference type="Ensembl" id="ENSEAST00005056241.1">
        <property type="protein sequence ID" value="ENSEASP00005050910.1"/>
        <property type="gene ID" value="ENSEASG00005007069.2"/>
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        <property type="entry name" value="CCDC14"/>
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        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 14"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="CCDC14"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="64"/>
            <end position="86"/>
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            <begin position="137"/>
            <end position="199"/>
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            <begin position="788"/>
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            <begin position="828"/>
            <end position="851"/>
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        <location>
            <begin position="489"/>
            <end position="570"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="14"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="70"/>
            <end position="83"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="172"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="838"/>
            <end position="851"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="957" mass="106474" checksum="2E9A441058ED746E" modified="2023-09-13" version="1">MERDLGGAPETVRRGVRRAPSRKRKLGGRAKRGRESAAVDSFYREVLLHCVWASLRLREMVRSGSRPGQVLSSGKQTGPSKLTNGKKGIHLRKTSHFNADSGYPIHSDSESQTETVQGLDGCASLLRDILRNEDSGSEIAYSENRSNPRPLEGKRYRSKKRGCGKHTVPSAVRREILSSDNKKQVPNEASAGSERDSSDVVQNWSLQDHYRMYSPIIYQALCEHVQTQMSLMNNLASKNSPNGISAVPCHSVSGSESQATLHSGYGLSTSTPVRLPQQPPCPPMVHSEVQTDGDNQFAPQDKTISVDCTDVQKNSFRTGLGFPRNLCKTDKPVVPTFQQLGLADGILPQQGVSKEPDLLKCFQTYMNLLHSHPDSQIRQSPTLLQPVFLATNEEKCAKEQIGEVTSEGKDLKICVHDSRIKDVQKAKNMNQSAEKVRTIKYLLGELKALVTEQEDPELQRLVTELEACISLLPAISGNTNIQVEIALAVQPLRSENAQLRRQLRILNQKLKEQEKTQKTSGSLQCNLELFSLQSLNKSLQNQLQESLKSQELLQSKNEELLKVVENQKDENKKFAGIFKEKDQTLLEHKQQFDIETTRMKIELEEALVNVKSSQFKLETVEKENQLLGITLRQRDAEVTRLRELTRTLQSSMAKLLSDLSMDSAHCKPGNNLTKSLLNIYEKQPQHDPIPAHTSIMSYLNKLETGHTFTRSELLSTIDNEENMVPDRPYENVLPPKGPQPRDTKNMEEVCAPGIISTLSKHGSDEESEIKTLIEDECNLDNTIYIPFARSTSKEKPPLSKRVSPQPQISVAPPQLVSSSALVSEKENKLCAPRVSSSSKKEAEVAPEKLSRTADMEDKHLLEKIKEALCKIPAATGEPQVPAASHGPSTCRSSSIQVKSSAIPDGSFLNSDLMSDWSISSFSTFTSRDEQDFRNGLAALDANIARLQKSLRTSLLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JPW6</accession>
    <name>A0A9L0JPW6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Adhesion G protein-coupled receptor E5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">Leukocyte antigen CD97</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">ADGRE5</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
            <location evidence="1">Extracellular space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JPW6"/>
    <dbReference type="Ensembl" id="ENSEAST00005040922.1">
        <property type="protein sequence ID" value="ENSEASP00005052022.1"/>
        <property type="gene ID" value="ENSEASG00005023556.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160578"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007166">
        <property type="term" value="P:cell surface receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000177">
        <property type="entry name" value="Adhesion G protein-coupled receptor E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000216">
        <property type="entry name" value="Adhesion G protein-coupled receptor E2"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.10.25.10:FF:000269">
        <property type="entry name" value="Adhesion G protein-coupled receptor E2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Adhesion G protein-coupled receptor E2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Adhesion G protein-coupled receptor E5"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="fibrillin-1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.220.50">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Laminin"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001881">
        <property type="entry name" value="EGF-like_Ca-bd_dom"/>
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    <dbReference type="InterPro" id="IPR000152">
        <property type="entry name" value="EGF-type_Asp/Asn_hydroxyl_site"/>
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    <dbReference type="InterPro" id="IPR018097">
        <property type="entry name" value="EGF_Ca-bd_CS"/>
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    <dbReference type="InterPro" id="IPR057244">
        <property type="entry name" value="GAIN_B"/>
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    <dbReference type="InterPro" id="IPR046338">
        <property type="entry name" value="GAIN_dom_sf"/>
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    <dbReference type="InterPro" id="IPR017981">
        <property type="entry name" value="GPCR_2-like_7TM"/>
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    <dbReference type="InterPro" id="IPR003056">
        <property type="entry name" value="GPCR_2_ADGRE2_ADGRE5"/>
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    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
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    <dbReference type="InterPro" id="IPR017983">
        <property type="entry name" value="GPCR_2_secretin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049883">
        <property type="entry name" value="NOTCH1_EGF-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011:SF348">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR E5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12011">
        <property type="entry name" value="ADHESION G-PROTEIN COUPLED RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
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    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
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    <dbReference type="PRINTS" id="PR01278">
        <property type="entry name" value="CD97PROTEIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00179">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00303">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00010">
        <property type="entry name" value="ASX_HYDROXYL"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01187">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00650">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50261">
        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50221">
        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="16" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6 23" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="14" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="18" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="15 24" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-0675">Receptor</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
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        <source>
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    <sequence length="241" mass="25251" checksum="B91FAF318FA66C20" modified="2023-09-13" version="1">IQVVGLRKRQPLGSTSRRVLSPAGRGPGSPRPRPARAQPRPWPSRGRPHRENQLFRCARVSQLGAAPLCPAGSPRLLGVASGPHGAPGPHNFSRTLPPAGKWPRDVIGARRPPARKRDAGRCGVTVVMEPLGLPGAGTLRGLACRDGQGFLRGGAASRVWTGHKCCSTGCGRICRGDIEGGRDGECPQVFLGLCIFNCVSDANCEAGEKCCKSGCGRFCVPAVLLSLAGHETQVGITSDSE</sequence>
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    <accession>A0A9L0JSR6</accession>
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                <person name="Jordana"/>
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                <person name="M."/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the G-protein coupled receptor 1 family.</text>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="12">Gamma-delta TR is a heterodimer composed of a gamma and delta chain; disulfide-linked. The gamma-delta TR is associated with the transmembrane signaling CD3 coreceptor proteins following the stoichiometry: a single gamma-delta TR heterodimer associates with one CD3D-CD3E heterodimer, one CD3G-CD3E heterodimer and one CD247 homodimer forming a stable octameric structure. Upon activation, gamma-delta TR complex associates with FCER1G to initiate intracellular signaling.</text>
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    <comment type="subcellular location">
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            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
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        <property type="component" value="Chromosome 1"/>
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    <dbReference type="GO" id="GO:0042101">
        <property type="term" value="C:T cell receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002250">
        <property type="term" value="P:adaptive immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:001866">
        <property type="entry name" value="T cell receptor gamma variable 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051117">
        <property type="entry name" value="TRG_var/const_region"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19256:SF65">
        <property type="entry name" value="T CELL RECEPTOR GAMMA CONSTANT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19256">
        <property type="entry name" value="T-CELL RECEPTOR GAMMA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0391">Immunity</keyword>
    <keyword evidence="11" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0732">Signal</keyword>
    <keyword evidence="13" id="KW-1279">T cell receptor</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="14">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040192600" description="Ig-like domain-containing protein" evidence="14">
        <location>
            <begin position="21"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="15">
        <location>
            <begin position="14"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00038578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005057489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="116" mass="13019" checksum="392C85F084B21E5D" modified="2023-09-13" version="1">MLWALALLLAFLAPATQVSSNLEGKQMSLTRQAGLSAVITCDIEHTSNYIHWYQYQEGMAPRRLLYYQVFSSNVMVESGITSGKYHAYGGTGRTCKFSLENLEESDSGVYYCAVWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="10" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JZL7</accession>
    <name>A0A9L0JZL7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methanethiol oxidase</fullName>
            <shortName evidence="3">MTO</shortName>
            <ecNumber evidence="3">1.8.3.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Selenium-binding protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">LOC106846504</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the oxidation of methanethiol, an organosulfur compound known to be produced in substantial amounts by gut bacteria. Selenium-binding protein which may be involved in the sensing of reactive xenobiotics in the cytoplasm. May be involved in intra-Golgi protein transport.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>methanethiol + O2 + H2O = hydrogen sulfide + formaldehyde + H2O2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11812"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:16007"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:16842"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="EC" id="1.8.3.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Organosulfur degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with USP33.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
        </subcellularLocation>
        <text evidence="3">May associate with Golgi membrane. May associate with the membrane of autophagosomes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the selenium-binding protein family.</text>
    </comment>
    <dbReference type="EC" id="1.8.3.4" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0JZL7"/>
    <dbReference type="Ensembl" id="ENSEAST00005047688.1">
        <property type="protein sequence ID" value="ENSEASP00005055517.1"/>
        <property type="gene ID" value="ENSEASG00005024206.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000014244"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018549">
        <property type="term" value="F:methanethiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008430">
        <property type="term" value="F:selenium binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008826">
        <property type="entry name" value="Se-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23300">
        <property type="entry name" value="METHANETHIOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23300:SF0">
        <property type="entry name" value="METHANETHIOL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05694">
        <property type="entry name" value="SBP56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75011">
        <property type="entry name" value="3-carboxy-cis,cis-mucoante lactonizing enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0711">Selenium</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU369071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005055517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="515" mass="57288" checksum="C8C829A941DD4114" modified="2023-09-13" version="1">MGLLQEGTRESRLAALAGGWGVCCSKQERRLQGLLLEPSGNTPVVFAATKCGDCGPGYPTPQEAMKGPREEIVYLPCIYRNTGTEAPDYLATVDVDPKSPQYSQVIHRLPMPHLKDELHHSGWNTCSSCFGDSTKSRNKLVLPSFISSRIYVVDVGTEPRAPKLHKVIEAKDIHEKCGLGTPHTSHCLPTGEVMISTLGDPKGNGKGGFVLLDGETFEVKGTWERPGGSAPMGYDFWYQPRHNVMISTEWAAPNVLRDGFNPADVEAGLYGKHLHVWDWQRHEIVQTLLMPDGHIPLEIRFLHDPAASQGFVGCALSSTIQRTWSVEKVIEVPPKKVKGWMLPEMPGLITDILLSLDDRFLYFNNWLHGDMRQYDISDPKRPRLVGQIFLGGSIVKGGPVQVLEDQELKSQPEPLVVKRLSTPLGFPWCPQMIQLSLDGKRIYVTTSLYSDWDKQFYPDLIREGSVMLQIDVDTVKGGLKLNPNFLVDFGKEPLGPALAHEIRYPGGDCTSDIWL</sequence>
</entry>
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    <accession>A0A9L0K6P5</accession>
    <name>A0A9L0K6P5_EQUAS</name>
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        <recommendedName>
            <fullName evidence="14">alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">ST6GALNAC2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>a 3-O-[N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + CMP-N-acetyl-beta-neuraminate = a 3-O-[N-acetyl-alpha-neuraminosyl-(2-&gt;6)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:81643"/>
            <dbReference type="Rhea" id="RHEA-COMP:11689"/>
            <dbReference type="Rhea" id="RHEA-COMP:19720"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:87075"/>
            <dbReference type="ChEBI" id="CHEBI:231970"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:81644"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 3-O-[N-acetyl-alpha-neuraminyl-(2-&gt;3)-beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl]-L-threonyl-[protein] + CMP-N-acetyl-beta-neuraminate = a 3-O-{alpha-Neu5Ac-(2-&gt;3)-beta-D-Gal-(1-&gt;3)-[alpha-Neu5Ac-(2-&gt;6)]-alpha-D-GalNAc}-L-threonyl-[protein] + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:81659"/>
            <dbReference type="Rhea" id="RHEA-COMP:14417"/>
            <dbReference type="Rhea" id="RHEA-COMP:16763"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:139598"/>
            <dbReference type="ChEBI" id="CHEBI:156398"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:81660"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a beta-D-galactosyl-(1-&gt;3)-N-acetyl-alpha-D-galactosaminyl derivative + CMP-N-acetyl-beta-neuraminate = a beta-D-galactosyl-(1-&gt;3)-[N-acetyl-alpha-neuraminyl-(2-&gt;6)]-N-acetyl-alpha-D-galactosaminyl derivative + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11136"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57812"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:133470"/>
            <dbReference type="ChEBI" id="CHEBI:140764"/>
            <dbReference type="EC" id="2.4.3.3"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:11137"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyltransferase 29 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.3.3" evidence="14"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0K6P5"/>
    <dbReference type="Ensembl" id="ENSEAST00005057922.1">
        <property type="protein sequence ID" value="ENSEASP00005061014.1"/>
        <property type="gene ID" value="ENSEASG00005004280.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160433"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001665">
        <property type="term" value="F:alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006493">
        <property type="term" value="P:protein O-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1480.20:FF:000013">
        <property type="entry name" value="ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1480.20">
        <property type="entry name" value="Glycosyl transferase family 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001675">
        <property type="entry name" value="Glyco_trans_29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038578">
        <property type="entry name" value="GT29-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45941:SF5">
        <property type="entry name" value="ALPHA-N-ACETYLGALACTOSAMINIDE ALPHA-2,6-SIALYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45941">
        <property type="entry name" value="ALPHA-N-ACETYLGALACTOSAMINIDE ALPHA-2,6-SIALYLTRANSFERASE 2-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00777">
        <property type="entry name" value="Glyco_transf_29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="72"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00036348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00050664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="437" mass="48236" checksum="FF86251338F81685" modified="2023-09-13" version="1">GRHSRLRSRRAAPEGAGLGRPGPGEQERQGGEGPRGQRAGRAWRGGAGRGRSGRPPPGARPGPPKPLPSARDGGGQGARGSGLHGAPARPVLLAAAPDRGCQLRDPLRPVLVGGAAVPGAPVQSQSLRCRHPLSLNIRRHPHFRGLFNLSTPVLLSWPLFTQELWDSLSQHKAPYGWQGLSRQAITSTLRLLNGSESTELFTGSREPRPHCIRCAVVGNGGILNGSRQGRNIDAHDYVFRLNGAVIKGFEDDVGTKTSFYGFTVNTMKNSLISYWTLGFTSVPQGQDLHYIFIPSDIRDYVMLRSAILGVRVPEGPDKGDRPWTYFGPEASASKFKLLHPDFISYLTERFLKSKLINTTFGDLYMPSTGALMLLTALHTCDQVSAYGFITRNYWKFSDHYFERIKKPLIFYANHDLSLEATLWRDLHKAGILWLYQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K6R0</accession>
    <name>A0A9L0K6R0_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106825595</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K6R0"/>
    <dbReference type="Ensembl" id="ENSEAST00005042480.1">
        <property type="protein sequence ID" value="ENSEASP00005061156.1"/>
        <property type="gene ID" value="ENSEASG00005035862.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154693"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000024">
        <property type="entry name" value="zinc finger protein 140 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001586">
        <property type="entry name" value="Zinc finger protein 354B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000200">
        <property type="entry name" value="zinc finger protein 510 isoform X2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000281">
        <property type="entry name" value="Zinc finger protein 558 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000098">
        <property type="entry name" value="Zinc finger protein 614"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000011">
        <property type="entry name" value="zinc finger protein 615 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001697">
        <property type="entry name" value="zinc finger protein 623"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001489">
        <property type="entry name" value="Zinc finger protein interacting with ribonucleoprotein K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF51">
        <property type="entry name" value="ZINC FINGER PROTEIN 833-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="14"/>
            <end position="92"/>
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    <sequence length="599" mass="68238" checksum="58E94B85F2DEE2E6" modified="2023-09-13" version="1">MAAAARREPAQGCVTFEDVVIYFSQEEWVLLDEAQRILYHDVMLENFVLTTSLDCWCGAEDEEIPSEQSISVDAVSQIRTSKAGPLTQKTHPCEKCVSVLKDVFHLTELQTTYSGHKPCFGGVSRGFWFSANLQQYQKHGSGEEIFKMNIDWTSFVTNCRYHMSGKPLTCGESGKDFLATSGLLLHQATPNGEKPHSNIDCEEAFNSRKSYDKWIECRKVFSNTNTVHHQRVCTREGLDECSKCGNAFSCKYKFVQHQPIHIREWPYKCGECVKLFTHETYLIQHCKVHSGAKPYECIECGRSFSWKSDLIQHQRVHTGERPYACSECGKSFSQSSGLLRHKKAHTRARLHECAECGKAFGCKSKLVGHQRIHTGARPYECAECGKFFRQSSSLVQHQRIHTGARPYECIECGKSFSKKSVLIQHQRVHTGERPYECRECGKFFSQKSILIRHQRVHTGERPYECSECGKAFRCKSKLVGHQRIHSGARPYECSECGKFFRQSSSLVQHQRIHTGARPYKCGECGKSFSQSAILIQHQRVHTGERPYECNECGKSFSQSSSLIQHQRVHTGARPYECTECGKSFSQSSGLLQHRRAHLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K870</accession>
    <name>A0A9L0K870_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Neurobeachin-like protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">NBEAL2</name>
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    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Probably involved in thrombopoiesis. Plays a role in the development or secretion of alpha-granules, that contain several growth factors important for platelet biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat neurobeachin family.</text>
    </comment>
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        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="P:intracellular protein localization"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:platelet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="neurobeachin isoform X1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Neurobeachin-like protein 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
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    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
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    <dbReference type="InterPro" id="IPR000409">
        <property type="entry name" value="BEACH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036372">
        <property type="entry name" value="BEACH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050865">
        <property type="entry name" value="BEACH_Domain"/>
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    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR046851">
        <property type="entry name" value="NBCH_WD40"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031570">
        <property type="entry name" value="NBEA/BDCP_DUF4704"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046852">
        <property type="entry name" value="Neurobeachin_a-sol"/>
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    <dbReference type="InterPro" id="IPR023362">
        <property type="entry name" value="PH-BEACH_dom"/>
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    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
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    <dbReference type="PANTHER" id="PTHR13743">
        <property type="entry name" value="BEIGE/BEACH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13743:SF111">
        <property type="entry name" value="NEUROBEACHIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02138">
        <property type="entry name" value="Beach"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15787">
        <property type="entry name" value="DUF4704"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF16057">
        <property type="entry name" value="DUF4800"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20426">
        <property type="entry name" value="NBCH_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20425">
        <property type="entry name" value="Neurobeachin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14844">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM01026">
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        <property type="match status" value="1"/>
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    <dbReference type="SMART" id="SM00320">
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        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
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        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81837">
        <property type="entry name" value="BEACH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50197">
        <property type="entry name" value="BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51783">
        <property type="entry name" value="PH_BEACH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 8" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="BEACH-type PH" evidence="11">
        <location>
            <begin position="1909"/>
            <end position="2034"/>
        </location>
    </feature>
    <feature type="domain" description="BEACH" evidence="10">
        <location>
            <begin position="2047"/>
            <end position="2339"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="2492"/>
            <end position="2525"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="2543"/>
            <end position="2584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1298"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1362"/>
            <end position="1435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1301"/>
            <end position="1311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1385"/>
            <end position="1395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1422"/>
            <end position="1434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00068540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="2748" mass="301419" checksum="1B548BC8E05B3F8B" modified="2023-09-13" version="1">MAASERLYELWLLYYAQKDLGYLQQWLTAFVSAFEKSISLSSLEPRRPEEAGAEVPLLPLDALHVLAEQLDKGDLEQALLLLKLFIILCRNLENVEAGWGPVLVPRVLALLTGLAAELKGPPSPQEGRGPQLENVALHALLLCEGLFDPYQTWRRQHSGEVISSKEKSKYKFPPATLPCEFSDFFRESLQDADRLPPKLLLRLIHLFGAVLAGGKENGQMAVSAGSVQGLLGVVRGWGHGPAQDPRLVPLALKALVGAVHILHASRTPPRGPELRALLEGYFRVLNADWPAGPSSGPEEGLITLRVSMLDAIPMMLACEDRPVLQATFLSNNCFEHLTRLIQNSKLYLQARAPPEGDSDLATRLLTEPDVQKVLDQDTDAIAVHVVRVLTCIMSSSPSAKEVFKERIGYPHLHEVLQSHGPPTHRLLQELLNMAVEGDHSICPPPPIRNEQPVLMLMQWLPALPTAELRLFLAQRLWWLCDSCPASRATCVQAGLVGCLLETLSTGVALGARCQEQLLALLQALGRVSLRPLELRHLLRPPPGLDSGPNGAEAENARHAGAIIRALSGMARHHGPARALRYFDLTPSMAGIMVPPVQRWPGPGFTFHAWLCLHPTAIAPAPAPTRPPQRKQLYSFFTSSGSGFEAFFTAAGTLVVAVCTRKEYLTVSLPEVSFVDSVWHCVAIVHVPGRRPFSQNLVHVYKDGHLVKTAPLRCPSLSEPFSSCCIGSAGHRTTTTTTGLPAPPVPAALAHTHPSLTRSQSVPVTTGLGWGSGLVAPLQEGSISSTLAGTQDTRWGSPTSLEGELGAVAIFHEALQAAALRVLCNLGPNETAPFKPEGDLHDLGTKLLLHYSPQACKNNICLDLSPGHGLDGRLTGHRVETWDVKDVVNCVGGMGALLPLLERVAAQPQDAEAGPAETHDLVGLELTSGHNTQGLLLPLGKSSEERMERNAVAAFLLMLRNFLQGHAVNQESLVQCQGPAIIGALLRKVPSWAMDMNVLMSTQMLMEQVAAEGSGSLLYLLYQHLLFNFHLWTLSDFAVRLGHIQYVSSIVREHRQKLRKKYGVQFILDALRTHYSPQRERPLAADDLRTVQTSLLGLAREFLARSSSTDDMQVVLSFLAAAGDDGQVVGTLDLLLALLQASSVQESLALFLLELGNLEVLLALLVRPSSLPLLPDRVCKILRRLQQNERLPERSRQRLRLRECGLQGLIACLPEGAVSPQLCQGLYKLFLGADCLNLSDLLAVVQLSLQADLSVRLDICRQLFHLIYGQPEIVRLLARQAGWQDVLTRLYVLEAATAGSPLPSPPEPPASPEPALFKPPTESPEPSDVFLPSEAPCPDPDPFYHALSPFCAPFDLGLERASVGSGNTAGGGSSSGTLTPASQPGTPSPLDGPRPFPAARGRHSSSLSNVLEDGSLPEPTISGDDTSNTSNPQQTSEEELCNLLTNVLFSVTWRGVEGSDEAAWRERGQVFSVLTQLGASATLVRPPDCIKRSLLEMMLESALTDIKEASLGVLASLTQQALWLLRLLQDFLCAEGHGNQELWSEKLFEGVCSLLDRLGAWPHLANGTADLREMAQIGLRLVLGYIVLEDPQLHAQAYVKLHSLLQTAVPMRREEACYVLSKLEAVLARALNTSPSEPATEDKKSPAAAAAAAERCSWLVPLVRTLLDRAYGPLGLQWGLPSLPPTNGSPTFFEDFQAFCVTPEWRHFIDKQVQPTMSQFEMDTYAKSHDLMSGFWNACYDMLMSSGQRRQRERAHSRRAFQELVLEPAQRRVRLEGLRYAAALKQQAAQHSTALLHWGALWRQLSSPCGAWALRDPPTPRWKVSSAETYSRMRLKLVPNHHFNPHLEASALRDNLGEAPLTPTQEASLPLAMTKEAKVSTLPEELQEDELGEEELAALETAMEAAELDEQHEKLVLSAECQLVTVVAVVPGLLEVTTQHVYFYDGSAERVETEEGIGHDFRRPLAQLREVHLRRFNLRRSALELFFIDQANYFLNFPSKVGGATASSPSQAPRPQPCPIPSHTQVRNQVYSWLLRLRPPTQGYLSSRSPQEMLRASGLTQKWVQREISNFEYLMQLNTIAGRTYNDLSQYPVFPWVLQDYVSPTLDLSNPAVFRDLSKPIGVVNPKHAQLVREKYESFEDPAGTIDKFHYGTHYSNAAGVMHYLIRMEPFTSLHVQLQSGRFDCSDRQFHSVAAAWQARLESPADVKELIPEFFYFPDFLENQNGFDLGCLQLTNEKVGDVVLPPWASSPEDFIQQHRRALESEYVSSHLHEWIDLIFGYKQRGPAAEEALNVFYYCTYEGAVDLDHVADERERKALEGIISNFGQTPCQLLKEPHPARLSAEEAAQRLARLDTNSPSIFQHLDQLKAFFAEVISDGVPLVLALVPHRQPHSFITQGSPDLLVTVSASGLLGTHTWLPYDRNISNYFSFSKDPTMGNPKMQRLLSGPWVPGSGVSGQALAVAPDGKLLFSGGHWDGSLRVTALPRGKLLNQLSRHLDVVTCLALDTCGIYLISGSRDTTCMVWRLLQQGGLSVGLASKPVQVLYGHEAAVSCVAISTELDMAVSGSEDGTVIIHTVRHGQFVAALRPPGASLPGPVSYLVLGSEGQIVVQSSARERLGAQVTYSLHLYSVNGRLRASLPLVEQPTALAVTEDFVLLGTAQCALHILHLNKLLPAAPPLPMKVPIRSVAVTKERSHVLVGLEDGKLIVVGAGQPSEVRSSQFARKLWRSSRRISQVSSGETEYNPGEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KAG5</accession>
    <name>A0A9L0KAG5_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">LOC106828270</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the alpha/beta interferon family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014692090.2">
        <property type="nucleotide sequence ID" value="XM_014836604.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0KAG5"/>
    <dbReference type="Ensembl" id="ENSEAST00005072086.1">
        <property type="protein sequence ID" value="ENSEASP00005058265.1"/>
        <property type="gene ID" value="ENSEASG00005032526.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106828270"/>
    <dbReference type="KEGG" id="eai:106828270"/>
    <dbReference type="GeneTree" id="ENSGT01000000214430"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 23"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005125">
        <property type="term" value="F:cytokine activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005126">
        <property type="term" value="F:cytokine receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.10:FF:000001">
        <property type="entry name" value="Interferon alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009079">
        <property type="entry name" value="4_helix_cytokine-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000471">
        <property type="entry name" value="Interferon_alpha/beta/delta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691:SF37">
        <property type="entry name" value="INTERFERON OMEGA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11691">
        <property type="entry name" value="TYPE I INTERFERON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00143">
        <property type="entry name" value="Interferon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00266">
        <property type="entry name" value="INTERFERONAB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00076">
        <property type="entry name" value="IFabd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47266">
        <property type="entry name" value="4-helical cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00252">
        <property type="entry name" value="INTERFERON_A_B_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="2 7" id="KW-0202">Cytokine</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040324703" evidence="8">
        <location>
            <begin position="22"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005058265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="195" mass="21918" checksum="076F344AAB015F6E" modified="2023-09-13" version="1">MAFSVSSLMALVVISSSPVSSMSCDLPASLDLRKQETLRILHQMETISPLSCLKHRTDFKFPQEQLDGSQFPEAQAMSVLQEMLQQTFNLFHTERSSAAWNTTLLDTLLTGLHQQLEDLNTCLDEQRGEEESALGTVDPTLAVKRYFRRIRLYLTEKKYSGCAWEIVRVEIMRSISSSANLQGRLGMKDGDLGSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KC48</accession>
    <name>A0A9L0KC48_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mediator of RNA polymerase II transcription subunit 31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">MED31</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7 9">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Mediator complex subunit 31 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KC48"/>
    <dbReference type="Ensembl" id="ENSEAST00005059927.1">
        <property type="protein sequence ID" value="ENSEASP00005062960.1"/>
        <property type="gene ID" value="ENSEASG00005027204.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015531"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:limb development"/>
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        <property type="term" value="P:hair follicle development"/>
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    <dbReference type="GO" id="GO:0035878">
        <property type="term" value="P:nail development"/>
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    <dbReference type="GO" id="GO:0043587">
        <property type="term" value="P:tongue morphogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000130">
        <property type="entry name" value="Homeobox protein Hox-D12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051003">
        <property type="entry name" value="AP_axis_regulatory_Homeobox"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022067">
        <property type="entry name" value="HoxA13_N"/>
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    <dbReference type="PANTHER" id="PTHR45804:SF5">
        <property type="entry name" value="HOMEOBOX PROTEIN HOX-C13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45804">
        <property type="entry name" value="SEGMENTATION PROTEIN FUSHI TARAZU-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="SMART" id="SM00389">
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="5 9 10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 9 10" id="KW-0371">Homeobox</keyword>
    <keyword evidence="8 9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Homeobox" evidence="12">
        <location>
            <begin position="243"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="9">
        <location>
            <begin position="245"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
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            <begin position="26"/>
            <end position="46"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004123"/>
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            <dbReference type="ARBA" id="ARBA00006317"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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            <dbReference type="RuleBase" id="RU000682"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="315" mass="34024" checksum="1B488357BB14AD2F" modified="2023-09-13" version="1">MRTARRRAAAAAAAAAAAGVRRRGAAAGGGCSGASPGKAQSMDGLGSSCPASHCRDLLPHPVLGRPPAPLGAPQGSVYTDIPTPEAARQCAPPPAPPTSSSATLGYGYPFGGSYYGCRLSHNVNLQQKPCAYHPGDKYPEPSGALPGDDLSSRAKEFAFYPSFASSYQAMPGYLDVSVVPGISGHPEPRHDALIPVEGYQHWALSNGWDSQVYCSKEQSQSAHLWKSPFPDVVPLQPEVSSYRRGRKKRVPYTKVQLKELEKEYAASKFITKEKRRRISATTNLSERQVTIWFQNRRVKEKKVVSKSKAPHLHST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KKZ1</accession>
    <name>A0A9L0KKZ1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transmembrane protein 126A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">TMEM126A</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Protein required for the cotranslational protein quality control in the inner membrane of the mitochondria. Associates with newly synthesized polypeptides and may act as a chaperone that cooperates with OXA1L for the insertion of newly synthesized mitochondrial proteins into the inner membrane. Required for the assembly of the ND4 module of mitochondrial complex I.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with OXA1L; promoting cotranslational quality control in mitochondria.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TMEM126 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014723509.1">
        <property type="nucleotide sequence ID" value="XM_014868023.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0KKZ1"/>
    <dbReference type="Ensembl" id="ENSEAST00005068171.1">
        <property type="protein sequence ID" value="ENSEASP00005062520.1"/>
        <property type="gene ID" value="ENSEASG00005023675.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106848252"/>
    <dbReference type="KEGG" id="eai:106848252"/>
    <dbReference type="CTD" id="84233"/>
    <dbReference type="GeneTree" id="ENSGT00520000055616"/>
    <dbReference type="OMA" id="GDLHCET"/>
    <dbReference type="OrthoDB" id="6234762at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141164">
        <property type="term" value="P:mitochondrial protein quality control"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021554">
        <property type="term" value="P:optic nerve development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032979">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane from matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034142">
        <property type="term" value="P:toll-like receptor 4 signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009801">
        <property type="entry name" value="TMEM126"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16296:SF4">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 126A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16296">
        <property type="entry name" value="UNCHARACTERIZED HYPOTHALAMUS PROTEIN HT007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07114">
        <property type="entry name" value="TMEM126"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005062520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="196" mass="21650" checksum="4CBCE54DE86EC4C3" modified="2023-09-13" version="1">MEDHEPDDTVKEKIFYIITRKINQLPEAERNLLEHGSTYIGLNAALCGLIANSLFRRVLNVTQARIAAGLPMAVIPFLTAHLSYKGFVSFPLNTGDLNCETCTITRGGLVGLVFGGLYPVILAIPVNGGLAARYESAPLPEKGNILTYWTRISKPIFRKMLFPILLQTGFAAYLGSRQYKLVIKALQLPEPGLEFQ</sequence>
</entry>
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    <accession>A0A8C4KR98</accession>
    <name>A0A8C4KR98_EQUAS</name>
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        <recommendedName>
            <fullName evidence="7">TELO2-interacting protein 1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">TTI1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Regulator of the DNA damage response (DDR). Part of the TTT complex that is required to stabilize protein levels of the phosphatidylinositol 3-kinase-related protein kinase (PIKK) family proteins. The TTT complex is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), ultraviolet (UV) and mitomycin C (MMC). Together with the TTT complex and HSP90 may participate in the proper folding of newly synthesized PIKKs. Promotes assembly, stabilizes and maintains the activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the tti1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044604726.1">
        <property type="nucleotide sequence ID" value="XM_044748791.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KR98"/>
    <dbReference type="Ensembl" id="ENSEAST00005000452.2">
        <property type="protein sequence ID" value="ENSEASP00005000365.1"/>
        <property type="gene ID" value="ENSEASG00005000367.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835066"/>
    <dbReference type="KEGG" id="eai:106835066"/>
    <dbReference type="CTD" id="9675"/>
    <dbReference type="GeneTree" id="ENSGT00390000009748"/>
    <dbReference type="OMA" id="PHPKKPW"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110078">
        <property type="term" value="C:TTT Hsp90 cochaperone complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000003">
        <property type="term" value="P:positive regulation of DNA damage checkpoint"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032006">
        <property type="term" value="P:regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000229">
        <property type="entry name" value="TELO2-interacting protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000240">
        <property type="entry name" value="TELO2-interacting protein 1 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052587">
        <property type="entry name" value="TELO2-interacting_protein_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057567">
        <property type="entry name" value="TPR_TTI1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057566">
        <property type="entry name" value="TPR_TTI1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016441">
        <property type="entry name" value="Tti1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049362">
        <property type="entry name" value="TTI1_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460">
        <property type="entry name" value="TEL2 INTERACTING PROTEIN 1 TTI1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18460:SF3">
        <property type="entry name" value="TELO2-INTERACTING PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24176">
        <property type="entry name" value="TPR_TTI1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24181">
        <property type="entry name" value="TPR_TTI1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24173">
        <property type="entry name" value="TPR_TTI1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21547">
        <property type="entry name" value="TTI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005250">
        <property type="entry name" value="UCP005250"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="TTI1 N-terminal TPR" evidence="9">
        <location>
            <begin position="11"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="TTI1 C-terminal TPR" evidence="10">
        <location>
            <begin position="759"/>
            <end position="1046"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="828"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="842"/>
            <end position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00056900"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00061544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00071526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF24181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1092" mass="121931" checksum="0924FE4C86F7BD41" modified="2022-01-19" version="1">MAVFDTPEEAFGVLRPVCVQLTKTQTVENVERLQAQLQAVSDSALQELQQYILFPLRFTLKAPGPKRERLIQSVVECLTFVLSSTCVKEQELLQELFSELSACLYSPTSQKPAAVSEELKLAVIQGLSTLMHSAYGDILVTFYEPSILPRLGFAVSLLLGLAEQEKSKQIKIAALKCLQVLLFQCDCEDHPRSLDELEQKQLGDLFAAFLPGISTALTRVITGDFKQGHSIVVSSLQIFYKTVGFVMADEQLRRLSKVQAKPAVEHRVVELMVHREADWVKNTSDKLTILIKKIIECVSVHPHWKVRLELIDLVEALLVKCSQSLVESVGPLLKALVSLVNDESSEVQAHCSKALRHFADQKVVVGNRAFADILSENLHSLATSLPRLMNSQDDQGKFSTLSLLLGYLKLLGPRVNFVLNSVSHLQRLSKALIQVLELDVADIKVVEERRWNSGHLSASPKTSAAGPRRQIQRRYFRFFTDERVFLLLRQVCHLLGFYGNLYLLVDHFLELYHESAVYRKQAAMILNELVAGAAGLEVENLHEKHTETSPEELREIVTSILEEYTSQENWYLVTCIEAEEMGEELTVKQSGLQAITSGAHTCQVSSFLAFSNPSPTICSMNSNIWQICIQLEGIGHFAYALGKDFRLLLMSALYPVLEKAGDQTLLISQAAASAMMDICHACGYDSLQHLINQNSDYLVNGISLNLRHLSLHPHTPKVLEVMLQNSDASLLPLVADVVQDVLATLDQFYDKRATSFVSVLHALLAALARWFPDTGNLGQLREQSVGEEGSHLSQRLATLEEGLENTTTAEDIEQFLLNYLKEKDVADGNVSDFDNEEEEQADPPKLEENDTRPDMEPPLPVHIQIATDVMERCIHLLSDKNLKIRLKVLDVLDLCVVVLQSHKNQLLPLAHRTWPSLVRRLTNDDPLAVLRAFKVLRTLGGKCGDFLRSRFCKDVLPKLAGSLVTQAPVSARAGPVYSHTLAFKLQLAVLQGLGPLCEKLDLGEGDLNKVAAACLIYLSAKQPVKLQEAARSVFLHLMKADPDSTWFLLNELYCPMQFTPPHPSLHPVRFQGATEQQNPYSANVLRLLRELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KY38</accession>
    <name>A0A8C4KY38_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">OR8B4</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cell membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KY38"/>
    <dbReference type="Ensembl" id="ENSEAST00005003320.2">
        <property type="protein sequence ID" value="ENSEASP00005003040.2"/>
        <property type="gene ID" value="ENSEASG00005002331.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01010000222320"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15405">
        <property type="entry name" value="7tmA_OR8B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000004">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 14" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 14" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="90"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="196"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="241"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="15">
        <location>
            <begin position="41"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003040.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="309" mass="34401" checksum="72C15E4440AB48F0" modified="2023-09-13" version="2">MTLRNSSSVTEFILMGLSEQSEFQLILFLLFLGIYVLTVVGNLGLITLIALSSSLHTPMYFFLFNLSFIDLCYSCVFTPQMLKDFVSENIISYMGCMAQLFFFCFFVNSECYVLISMAYDRYVAICNPLLYTVTMSPQVCSGLVFGSYVIGFAGAMAHTGSMLRLTFCDSNIIDHYLCEVLPLLQLFCTSTHVNKLVFFIVVGVVITLSSISIFISYAFILSNILCIPSAEGRSQAFSTCGSHIVAVAVFLGSGAFTYLTTSFPGSRDQRKFASVFYTNVVPMLNPLIYSLRNKDVKLALSKTLKRVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KY56</accession>
    <name>A0A8C4KY56_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nuclear pore complex protein Nup98-Nup96</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="25" type="primary">NUP98</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. NUP98 and NUP96 are involved in the bidirectional transport across the NPC. May anchor NUP153 and TPR to the NPC. In cooperation with DHX9, plays a role in transcription and alternative splicing activation of a subset of genes. Involved in the localization of DHX9 in discrete intranuclear foci (GLFG-body).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Nucleoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the nucleoporin GLFG family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044608158.1">
        <property type="nucleotide sequence ID" value="XM_044752223.2"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005000963.2">
        <property type="protein sequence ID" value="ENSEASP00005000829.1"/>
        <property type="gene ID" value="ENSEASG00005000732.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831644"/>
    <dbReference type="KEGG" id="eai:106831644"/>
    <dbReference type="CTD" id="4928"/>
    <dbReference type="GeneTree" id="ENSGT00550000074799"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042405">
        <property type="term" value="C:nuclear inclusion body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044614">
        <property type="term" value="C:nuclear pore cytoplasmic filaments"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044615">
        <property type="term" value="C:nuclear pore nuclear basket"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140693">
        <property type="term" value="F:molecular condensate scaffold activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990841">
        <property type="term" value="F:promoter-specific chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017056">
        <property type="term" value="F:structural constituent of nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051292">
        <property type="term" value="P:nuclear pore complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048026">
        <property type="term" value="P:positive regulation of mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034398">
        <property type="term" value="P:telomere tethering at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.2360:FF:000001">
        <property type="entry name" value="Nuclear pore complex protein Nup98-Nup96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.690:FF:000001">
        <property type="entry name" value="Nuclear pore complex protein Nup98-Nup96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1610.10:FF:000001">
        <property type="entry name" value="Nuclear pore complex protein Nup98-Nup96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.690">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1610.10">
        <property type="entry name" value="Peptidase S59, nucleoporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037665">
        <property type="entry name" value="Nucleoporin_S59-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007230">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036903">
        <property type="entry name" value="Nup98_auto-Pept-S59_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021967">
        <property type="entry name" value="Nup98_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198:SF6">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN NUP98-NUP96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23198">
        <property type="entry name" value="NUCLEOPORIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04096">
        <property type="entry name" value="Nucleoporin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12110">
        <property type="entry name" value="Nup96"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21240">
        <property type="entry name" value="Nup98_GLEBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82215">
        <property type="entry name" value="C-terminal autoproteolytic domain of nucleoporin nup98"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51434">
        <property type="entry name" value="NUP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="20" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="19" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-0653">Protein transport</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="14" id="KW-0720">Serine protease</keyword>
    <keyword evidence="18" id="KW-0811">Translocation</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="15" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Peptidase S59" evidence="24">
        <location>
            <begin position="738"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="512"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="608"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="663"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="885"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="525"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="23">
        <location>
            <begin position="670"/>
            <end position="683"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004620"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00059309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1817" mass="197293" checksum="D662FD0D6BD8DB25" modified="2022-01-19" version="1">MFNKSFGTPFGGGTGGFGTTSTFGQNTGFGTTSGGAFGTSAFGSSNNTGGLFGNSQTKPGGLFGTNSFSQPATSTSTGFGFGTSTGTSNSLFGTASTGTSLFSSQNNAFAQNKPTGFGNFGTSTSSGGLFGTTNTTSNPFGSTSGSLFGPSSFTAAPTGTTIKFNPPTGTDTMVKAGVSTNISTKHQCITAMKEYESKSLEELRLEDYQANRKGPQNQVGAGTTTGLFGSSPATSSATGLFSSSTTNSGFAYGQNKTAFGTSTTGFGTNPGGLFGQQNQQTTSLFGKPFAQAITTQNTGFSFGNTSTLGQPSTNTMGLFGVTQASQPGGLFGTATNTSTGTAFGTGTGLFGQTNTGFGAVGSTLFGNNKLTTFGTSTTSAPSFGTTSGGLFGNKPTLTLGTNTNTSNFGFGTNTSGNSIFGNKAGPGTLGTGLGAGFGTALGAGQASLFGNNQPKIGGPLGTGAFGAPGFNTTTATLGFGAPLAPVALTDPNASAAQQAVLQQHINSLTYSPFGDSPLFRNPMSDPKKKEERLKPTNPAAQKALTTPTHYKLTPRPATRVRPKALQTTGTAKSHLFDGLDDDEPSLANGAFMPKKSIKKLVLKNLNNSNLFSPVNRDSEDLASPSDYPENGERFSFLSKPVDENHQQDGDDDSLVSRFYTNPIAKPIPQTPESAGNKHNSSSSVDDTIVALNMRAALRNGLEGSSEETSFHEESLQDDREEIENNTYHMHPAGIVLTKVGYYTIPSMDDLAKITNEKGECIVSDFTIGRKGYGSIYFEGDVNLTNLNLDDIVHIRRKEVIVYLDDNQKPPVGEGLNRKAEVTLDGVWPTDKTSRCLIKSPDRLADINYEGRLEAVSRKQGAQFKEYRPETGSWVFKVSHFSKYGLQDSDEEEEERPSKTSTKKLKTAPLPPAGQATPFQMALNGKPAPPPQSQSPEVEQLGRVVELDSDMVDITQEPVLDSVLEESVPEDQEPVSASTHIASSLGINPHVLQIMKASLLADEEDVDTVLDQRFSRLPSKADTSQEICSPRLPISASHSSKSRSLVGGLLQSKFTSGAFLPPSASVQECRTPRASSLMNIPSTSPWSVPPTLTSVFSVPSPAPEVQLKTVGTRRQPGLVPLEKSVTYGKGKLLMDIALFMGRSFRVGWGPNWTLANSGEQLSGSHELENHQIADSMEYGFLPNPVAVKSLTESPFKVHLEKLSLRQKKPDEDLQLYQTPLELKLKHSTVHVDELCPLVVPNPGVAVIHDYADWVKEASGDLLEAQIVKHWSLTWTLCEALWGHLKELDSQLDEPSEYIQILERRRAFSRWLSQTAAPQIEEEVSLTRKDNPVEAVFSYLTGKRISEACSLAQQSGDHRLALLLSQLVGSQSIQELLTMQLVDWHQLQADCFIQDERLRIFALLAGKPVWQLSEQKQINVCSQLDWKRSLAVHLWYLLPPTASISTALSMYEEAFQNTSEGDRYACSPLPSYLEGSGCVLEEEQDSRRPLQDVCFHLLKLYSDRHYDLNQLLEPRSITADPLDYRLSWHLWEVLRALNYTHLSEQCEGVLQASYAGQLESEGLWEWAIFVLLHIDNSSMREKAVRELLTRHCQLLETPESWAKETFLTQKLCVPAEWIHEAKAVRAHMESNKHLEALYLFKAGHWNRCHKLVIRHLASDAIINENYDYLKGFLEDLAPPERSSLIQDWETSGLVYLDYIQVIEMLHHVQQVDCTGYELEQLHSKVTSLCNRIEQIQCYNAKDRLAQSDMAKRVANLLRVVLSLQHAPDTTSDSTPDPQRVPLRLLAPHIGRLPMPEDYALEELRSLTQSYLRELTVGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KZ30</accession>
    <name>A0A8C4KZ30_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Aminopeptidase</fullName>
            <ecNumber evidence="20">3.4.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="primary">NPEPPS</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Release of an N-terminal amino acid, preferentially alanine, from a wide range of peptides, amides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18 20">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="18 20">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 20">Belongs to the peptidase M1 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.-" evidence="20"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4KZ30"/>
    <dbReference type="Ensembl" id="ENSEAST00005002017.2">
        <property type="protein sequence ID" value="ENSEASP00005001815.2"/>
        <property type="gene ID" value="ENSEASG00005000896.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155246"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016285">
        <property type="term" value="F:alanyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042277">
        <property type="term" value="F:peptide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043171">
        <property type="term" value="P:peptide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09601">
        <property type="entry name" value="M1_APN-Q_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.390.10:FF:000001">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.50.20:FF:000004">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1730:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1910:FF:000002">
        <property type="entry name" value="Aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045357">
        <property type="entry name" value="Aminopeptidase_N-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024571">
        <property type="entry name" value="ERAP1-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034016">
        <property type="entry name" value="M1_APN-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001930">
        <property type="entry name" value="Peptidase_M1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050344">
        <property type="entry name" value="Peptidase_M1_aminopeptidases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014782">
        <property type="entry name" value="Peptidase_M1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533">
        <property type="entry name" value="PROTEASE M1 ZINC METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11533:SF174">
        <property type="entry name" value="PUROMYCIN-SENSITIVE AMINOPEPTIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11838">
        <property type="entry name" value="ERAP1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01433">
        <property type="entry name" value="Peptidase_M1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17900">
        <property type="entry name" value="Peptidase_M1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00756">
        <property type="entry name" value="ALADIPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 20" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 20" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 18 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13 20" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="14" id="KW-0944">Nitration</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 20" id="KW-0645">Protease</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 18 20" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminopeptidase N-like N-terminal" evidence="23">
        <location>
            <begin position="102"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M1 membrane alanine aminopeptidase" evidence="21">
        <location>
            <begin position="322"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="ERAP1-like C-terminal" evidence="22">
        <location>
            <begin position="620"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="17">
        <location>
            <position position="395"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="394"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="19">
        <location>
            <position position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00052895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR634016-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU364040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF11838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF17900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001815.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="961" mass="108376" checksum="33B7F7F1D418E9FF" modified="2023-09-13" version="2">MELIYFLFMKNTGWVGNILSTPHISNSHSNSHFKLQKRVKAGISWQQLPMDCSPTFCVPGLWNPFTIFCNRSSRRRRRLHSLGLAAMPEKRPFERLPADVSPINYSLCLKPDLLDFTFEGKLEAAAQVRQATNQIVMNCADIDIITASYAPEGDEEIHATGFNYQNEDEKVTLSFPSILQTGTGTLKIDFVGELNDKMKGFYRSKYTTPSGEVRYAAVTQFEATDARRAFPCWDEPAIKATFDISLIVPKDRVALSNMNVIDRKPYPDDENVVEVKFARTPVMSTYLVAFVVGEYDFVETRSKDGVCVRVYTPVGKAEQGKFALEVAAKTLPFYKDYFNVPYPLPKIDLIAIADFAAGAMENWGLVTYRETALLIDPKNSCSSSRQWVALVVGHELAHQWFGNLVTMEWWTHLWLNEGFASWIEYLCVDHCFPEYDIWTQFVSADYTRAQELDALDNSHPIEVSVGHPSEVDEIFDAISYSKGASVIRMLHDYIGDKDFKKGMNMYLTKFQQKNAATEDLWESLENASGKPIAAVMNTWTKQMGFPLIYVEAEQVEDDRLLRLSQRKFCASGPYVGEDCPQWMVPITISTSEDPNLAKLKILMDKPEMSVVLKNVKPDQWVKLNLGTVGFYRTQYSSAMLESLLPGIRDLSLPPVDRLGLQNDLFSLARAGIISTVEVLKVMEAFVNEPNYTVWSDLSCNLGILSTLLSHTDFYEEIQEFVKDVFSPIGERLGWDPKPGEGHLDALLRGLVLGKLGKAGHKATLEEARRRFKDHVEGKQILSADLRSPVYLTVLKHGDGTTLDIMLKLHKQADMQEEKNRIERVLGATPSPELIQKVLTFALSEEVRPQDTVSVIGGVAGGSKHGRKAAWKFIKDNWEELYNRYQGGFLISRLIKLSVEGFAVDKMAGEVKAFFESHPAPSAERTIQQCCENILLNAAWLKRDAESIHQYLLQRKASPATA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L0J6</accession>
    <name>A0A8C4L0J6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Filensin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Beaded filament structural protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">BFSP1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Required for the correct formation of lens intermediate filaments as part of a complex composed of BFSP1, BFSP2 and CRYAA. Involved in altering the calcium regulation of MIP water permeability.</text>
    </comment>
    <comment type="subunit">
        <text evidence="19">Part of a complex required for lens intermediate filament formation composed of BFSP1, BFSP2 and CRYAA. Identified in a complex that contains VIM, EZR, AHNAK, BFSP1, BFSP2, ANK2, PLEC, PRX and spectrin. Found in a complex composed of PPL (via C-terminal linker domain), BFSP1 and BFSP2 in the retinal lens. Within the complex interacts with BFSP2. Interacts (via C-terminus) with MIP (via C-terminus) in aged lens fiber cells.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cell cortex</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4L0J6"/>
    <dbReference type="Ensembl" id="ENSEAST00005002694.2">
        <property type="protein sequence ID" value="ENSEASP00005002447.2"/>
        <property type="gene ID" value="ENSEASG00005001908.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016976"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005882">
        <property type="term" value="C:intermediate filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005212">
        <property type="term" value="F:structural constituent of eye lens"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048469">
        <property type="term" value="P:cell maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070307">
        <property type="term" value="P:lens fiber cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000094">
        <property type="entry name" value="Beaded filament structural protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000009">
        <property type="entry name" value="filensin isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042358">
        <property type="entry name" value="BFSP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14069">
        <property type="entry name" value="FILENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14069:SF0">
        <property type="entry name" value="FILENSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="12" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="13 20" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8" id="KW-0273">Eye lens protein</keyword>
    <keyword evidence="11" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="16" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0519">Myristate</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="IF rod" evidence="22">
        <location>
            <begin position="39"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="355"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="447"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="494"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="57"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="20">
        <location>
            <begin position="217"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="360"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="375"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="500"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="530"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="21">
        <location>
            <begin position="573"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="585"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00056753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00064349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005002447.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="662" mass="73649" checksum="4BF3097E12F19A18" modified="2023-09-13" version="2">MYRRSYVFQTRKEQYERAEEASRAAEQDSLADGRAAAPGLVALQGLGERVAAHVQRARVLEQRHAVLRRQLDAFQRLDELAGPEDALARHVESNRQRVRDLAAERVRLERQGAEAQRALDEFRSKYENECECQLLLKEMLERLNKEADEALLHNLRLQIEAQFLQDDISAAKDRYKKNLLEIQTYVSILQQIVQTTPQVSAITSGMREEKLLTEREATALQSQLEEGREMLSFLQAQRAELQAQTATLEQAIKDAHDCYDGEIQLYNEQIESLRKEIEETERSLEKSSYDCRQLVVAQQTLKNELDRYHRIIENEGSRLSSAFIETPITLFTLSHGASVSPRTGGKDLTRAVQDITTAKPRQKGLPRNIPRKKIIAKDKAEESLEDAPPKDPEDTKLVQVVLKEEGESKLESGGEEASPPTPEETPEDVPDGGQISKAFGKLCKMVKERVRSPQEPEPPADLCTEEQHVLVSGDASYMNPEFCSFSMPARGQVVVSTEDDSVRRDSHAEPSPEQPKPPLEDGQGDSQGGEEGRPPDQHPAEKESEISAEELKDTGEKHDGQREEEGSQRPCPVVTPGPEGPSTPPSQTSVVSEGGSEGSGAQSSSLPERGPPRTLAYEKVQVVESIEKFSTESVQTYEETAVVVETVTGKTKAKKKLGETGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L1K2</accession>
    <name>A0A8C4L1K2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106830138</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
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            <authorList>
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            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
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    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
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    <dbReference type="EC" id="2.7.11.25" evidence="3"/>
    <dbReference type="RefSeq" id="XP_014685661.1">
        <property type="nucleotide sequence ID" value="XM_014830175.3"/>
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    <dbReference type="AlphaFoldDB" id="A0A8C4L8T0"/>
    <dbReference type="Ensembl" id="ENSEAST00005006025.2">
        <property type="protein sequence ID" value="ENSEASP00005005515.2"/>
        <property type="gene ID" value="ENSEASG00005004074.2"/>
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    <dbReference type="GeneID" id="106824083"/>
    <dbReference type="KEGG" id="eai:106824083"/>
    <dbReference type="CTD" id="4293"/>
    <dbReference type="GeneTree" id="ENSGT00940000158243"/>
    <dbReference type="OrthoDB" id="339325at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0004706">
        <property type="term" value="F:JUN kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd12059">
        <property type="entry name" value="SH3_MLK1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14145">
        <property type="entry name" value="STKc_MLK1"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.10.510.10:FF:000076">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
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    <dbReference type="FunFam" id="3.30.200.20:FF:000085">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035779">
        <property type="entry name" value="MLK1-3_SH3"/>
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    <dbReference type="InterPro" id="IPR016231">
        <property type="entry name" value="MLK1-4"/>
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    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
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    <dbReference type="InterPro" id="IPR001245">
        <property type="entry name" value="Ser-Thr/Tyr_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051681">
        <property type="entry name" value="Ser/Thr_Kinases-Pseudokinases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329:SF35">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44329">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE TNNI3K-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07714">
        <property type="entry name" value="PK_Tyr_Ser-Thr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000556">
        <property type="entry name" value="MAPKKK9_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00452">
        <property type="entry name" value="SH3DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00109">
        <property type="entry name" value="TYRKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
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    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4 15" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
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        <location>
            <begin position="50"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="20">
        <location>
            <begin position="142"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="17"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="532"/>
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    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="571"/>
            <end position="643"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="711"/>
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    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="781"/>
            <end position="827"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="897"/>
            <end position="1087"/>
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    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="421"/>
            <end position="488"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="540"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="571"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="730"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="792"/>
            <end position="804"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
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            <begin position="900"/>
            <end position="917"/>
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            <begin position="935"/>
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            <begin position="1021"/>
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            <begin position="1037"/>
            <end position="1048"/>
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    <feature type="active site" description="Proton acceptor" evidence="13">
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            <position position="266"/>
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    <feature type="binding site" evidence="14">
        <location>
            <begin position="148"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="14 16">
        <location>
            <position position="169"/>
        </location>
        <ligand>
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            <dbReference type="ARBA" id="ARBA00001946"/>
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    <evidence type="ECO:0000256" key="2">
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            <dbReference type="ARBA" id="ARBA00006529"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
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            <dbReference type="ARBA" id="ARBA00012406"/>
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    <evidence type="ECO:0000256" key="18">
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    <evidence type="ECO:0000313" key="22">
        <source>
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</entry>
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    <accession>A0A8C4L918</accession>
    <name>A0A8C4L918_EQUAS</name>
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            <fullName evidence="13">Olfactory receptor</fullName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A8C4L918"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:G protein-coupled receptor activity"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15225">
        <property type="entry name" value="7tmA_OR10A-like"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26453">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0552">Olfaction</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="11 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="104"/>
            <end position="126"/>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="261"/>
            <end position="283"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="295"/>
            <end position="314"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="326"/>
            <end position="344"/>
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    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="47"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
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            <dbReference type="ARBA" id="ARBA00022606"/>
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    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363047"/>
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    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005007715.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="356" mass="39912" checksum="F06EED455C1522C3" modified="2023-09-13" version="2">MEYEEIKAEDNVSTVIQFVLLGFSDLPNLQGLLFGVFSIMYIIILFGNCLIIIITRLDTAQQKPMYFFLSNFSSLEMCYVSVTLPRIPVNLWTQDRSISIRNCAAQMCFLLMLGATECFLLAVMAYDRYVAICNPLRYPLVMNQKMCIQLAIGSWISGIPVQIGQTCQIFSLHFCNSNQINHFFCDIPPILKLACGDTSIHEMSVYVVAMLFVAVPFMLILIQLPFCGLNVIDHFMCDLIPLLELPCTDTHTLGPLIAENSGSLCLLIFSMLVVSYIIILCSLSSEGRHKALSTCGSHVTVVILFFVLCSFLYLRPVTSFPIDKAVTVFCTIVTPTLNALIYTFRNAEVKNVMKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L9K4</accession>
    <name>A0A8C4L9K4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Zinc finger and SCAN domain-containing protein 10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Zinc finger protein 206</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="24" type="primary">ZSCAN10</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Embryonic stem (ES) cell-specific transcription factor required to maintain ES cell pluripotency. Can both activate and /or repress expression of target genes, depending on the context. Specifically binds the 5'-[GA]CGCNNGCG[CT]-3' DNA consensus sequence. Regulates expression of POU5F1/OCT4, ZSCAN4 and ALYREF/THOC4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with POU5F1/OCT4 and SOX2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014688724.3">
        <property type="nucleotide sequence ID" value="XM_014833238.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L9K4"/>
    <dbReference type="Ensembl" id="ENSEAST00005009217.2">
        <property type="protein sequence ID" value="ENSEASP00005008456.2"/>
        <property type="gene ID" value="ENSEASG00005006087.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106826081"/>
    <dbReference type="KEGG" id="eai:106826081"/>
    <dbReference type="CTD" id="84891"/>
    <dbReference type="GeneTree" id="ENSGT00940000162513"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07936">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000321">
        <property type="entry name" value="myeloid zinc finger 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000982">
        <property type="entry name" value="Zinc finger and SCAN domain containing 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001080">
        <property type="entry name" value="Zinc finger and SCAN domain containing 10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001341">
        <property type="entry name" value="Zinc finger and SCAN domain containing 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001786">
        <property type="entry name" value="Zinc finger and SCAN domain containing 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001917">
        <property type="entry name" value="Zinc finger and SCAN domain containing 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000512">
        <property type="entry name" value="zinc finger protein 197 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.4020.10:FF:000001">
        <property type="entry name" value="zinc finger protein 263 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000690">
        <property type="entry name" value="Zinc finger protein 354C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000290">
        <property type="entry name" value="Zinc finger protein 697 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="13"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4020.10">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003309">
        <property type="entry name" value="SCAN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038269">
        <property type="entry name" value="SCAN_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399:SF70">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24399">
        <property type="entry name" value="ZINC FINGER AND BTB DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02023">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00431">
        <property type="entry name" value="SCAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47353">
        <property type="entry name" value="Retrovirus capsid dimerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50804">
        <property type="entry name" value="SCAN_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="14"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0488">Methylation</keyword>
    <keyword evidence="14 20" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="10" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SCAN box" evidence="23">
        <location>
            <begin position="44"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="345"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="374"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="402"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="430"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="474"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="520"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="548"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="576"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="604"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="632"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="660"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="688"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="726"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="22">
        <location>
            <begin position="754"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="146"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="255"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="29"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="21">
        <location>
            <begin position="170"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="21">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="268"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="290"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00057877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00062677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00070945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00079281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS50804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008456.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="782" mass="86865" checksum="CE88A5A394AD1C07" modified="2023-09-13" version="2">MLAEPVSATREPEQLGAVKLEEEESAGQEDPRQPEARPRPEVAHQLFRCFQYQEDMGPRASLSRLRELCSHWLRPALHTKKQILELLVLEQFLSVLPPHLLARLQGQQLRDGEEVVLLLEGVQRESSNVGPLDFSFNAGKNCPRADITLEEQGGPSQVSSHSPKKEMPSEGPPAVEPPNELPPSQPGPSKPAELGAWRHSPSSKQPQSPGPKRTFQALQESAPQGPPLWPEENSRDQELAAVLESLTFEDVPAKKSWPVHPLGFGSRTPDEEFKEEPKGVAWPAATSAESRADSPRVAEEPHAPSLGQGPEVSGTGEAVSSPSKNDILEVKVAGEAPKSEPEMKVICTECGVSFPELARLETHQLRSHPGSRSFPCLCCGKSFGRSSILKLHMRTHTDERPHACHLCSHRFRQSSHLSKHLQTHSSEPAFLCAECGQGFQRRSSLMQHLLAHAQDQKLPCAPETKTEAPEPAVVLCSHCGQTFQRRSSLKRHLRIHAKDQGHQCSECSGSLRPGPERRPYVCSDCGKAFRRSEHLGAHRRVHTGERPFSCQVCGRSFSQSSQLICHQRVHTGEKPYGCPHCGKRFVRRAGLARHLLTHGGPRPHHCTQCGKSFSQTQDLARHRRSHTGEKPCRCSQCGEGFSQSAHLARHQRIHTGEKPHVCDTCGHRFRNSSNLARHRRSHTGERPYGCQTCGRSFRRNAHLQRHLATHAGAGDEAVNGQAEPPQECLECGKSFSRSCNLLRHMLVHTGARPYSCTQCGRGFSRNSHLLRHLRTHARETLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LJI2</accession>
    <name>A0A8C4LJI2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Syntaxin-binding protein 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Lethal(2) giant larvae protein homolog 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Tomosyn-1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">STXBP5</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat L(2)GL family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014706131.1">
        <property type="nucleotide sequence ID" value="XM_014850645.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LJI2"/>
    <dbReference type="Ensembl" id="ENSEAST00005013940.2">
        <property type="protein sequence ID" value="ENSEASP00005012828.1"/>
        <property type="gene ID" value="ENSEASG00005008967.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106837222"/>
    <dbReference type="CTD" id="134957"/>
    <dbReference type="GeneTree" id="ENSGT00950000182906"/>
    <dbReference type="OMA" id="IVWKFFD"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045159">
        <property type="term" value="F:myosin II binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15893">
        <property type="entry name" value="R-SNARE_STXBP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:003138">
        <property type="entry name" value="Syntaxin binding protein 5 like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000001">
        <property type="entry name" value="syntaxin-binding protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000186">
        <property type="entry name" value="syntaxin-binding protein 5-like isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000664">
        <property type="entry name" value="Lethal2_giant"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013905">
        <property type="entry name" value="Lgl_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013577">
        <property type="entry name" value="LLGL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042855">
        <property type="entry name" value="V_SNARE_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241">
        <property type="entry name" value="LETHAL 2 GIANT LARVAE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10241:SF22">
        <property type="entry name" value="SYNTAXIN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08596">
        <property type="entry name" value="Lgl_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08366">
        <property type="entry name" value="LLGL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00962">
        <property type="entry name" value="LETHAL2GIANT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50892">
        <property type="entry name" value="V_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="12 18" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <keyword evidence="9 17" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="17">
        <location>
            <begin position="233"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="V-SNARE coiled-coil homology" evidence="20">
        <location>
            <begin position="1050"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="557"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="674"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="845"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="17"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="845"/>
            <end position="856"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00069759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00075853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00081591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005012828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1115" mass="123306" checksum="41E0F614AE3A0200" modified="2022-01-19" version="1">MRKFNIRKVLDGLTAGSSSASQQQPQQHPPGTREPEIQETLQSEHFQLCKTVRHGFPYQPSALAFDPVQKILAVGTQTGALRLFGRPGVECYCQHESGAAVIQLQFLINEGALVSALADDTLHLWNLRQKRPAILHSLKFCRERVTFCHLPFQSKWLYVGTERGNIHIVNVESFTLSGYVIMWNKAIELSSKSHPGPVVHISDNPMDEGKLLIGFESGTVVLWDLKSKKADYRYTYDEAIHSVAWHHEGKQFICSHSDGTLTIWNVRSSAKPVQTITPHGKQLKDGKKPEPCKPILKVEFKTTRSGEPFIILSGGLSYDTVGRRPCLTVMHGKSTAVLEMDYSIVDFLTLCETPYPNDFQEPYAVVVLLEKDLVLIDLAQNGYPIFENPYPLSIHESPVTCCEYFADCPVDLIPALYSVGARQKRQGYSKKEWPINGGNWGLGTQSYPEIIITGHADGSVKFWDASAVTLQVLYKLKTSKVFEKSRNKDDRPNTDIVDEDPYAIQIISWCPESRMLCIAGVSAHVIIYRFSKQEVITEVIPMLEVRLLYEINDVESPEGEQAPPLPTPVGGANPQPIPPQSHPSTSSSSSDGLRDNVPCLKVKNSPLKQSPGYQTELVIQLVWVGGEPPQQITSLAVNSSYGLVVFGNCNGIAMVDYLQKAVLLNLGTIELYGSNDPYRREPRSPRKSRQPSGAGLCDISEGTAVPEDRCKSPTSAKMSRKLSLPTDLKPDLDIKDNSFSRSRSSSVTSIDKESREAISALHFCETFTRKADSSPSPCLWVGTTLGTVLVIALNLPPGGEQRLLQPVIVSPSGTILRLKGAILRMAFLDATGCLVPPAYESWREHNVPEEKDEKEKLKKRRPVSVSPSSSQEISENQYAVICSEKQAKVISLPTQNCAYKQNITETSFVLRGDVVAMSNSICLACFCANGHIMTFSLPSLRPLLDVYYLPLTNMRIARTFCFTNNGQALYLVSPTEIQRLTYSQETCENLQEMLGELFTPVETPEAPNRGFFKGLFGGGAQSLDREELFGESSSGKASRSLAQHIPGPGGIEGVKGAASGVVGELARARLALDERGQKLGDLEERTAAMLSSADAFSKHAHEMMLKYKDKKWYQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LK02</accession>
    <name>A0A8C4LK02_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">OR1D2</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LK02"/>
    <dbReference type="Ensembl" id="ENSEAST00005014186.2">
        <property type="protein sequence ID" value="ENSEASP00005013053.1"/>
        <property type="gene ID" value="ENSEASG00005035853.1"/>
    </dbReference>
    <dbReference type="KEGG" id="eai:106821986"/>
    <dbReference type="GeneTree" id="ENSGT00940000160386"/>
    <dbReference type="OMA" id="PFGFMII"/>
    <dbReference type="OrthoDB" id="6151005at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd15918">
        <property type="entry name" value="7tmA_OR1_7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000009">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48001">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="9 11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="99"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="41"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005013053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="313" mass="35303" checksum="B93CA93932AADF2C" modified="2022-01-19" version="1">MDGSNQSGVSEFLLLGLSESPEEQHVLFWMFLSMYLVTVVGNVLIILAIGFDSRLHTPMYFFLANLSFTDLFFVTNTIPKMLVNIQSQNKAISYAGCLTQLYFLVSLVALDNLILATMAYDRYVAICRPLHYTTAMSSRLCILLLALCWVFSVLYGLIHTLLMTRVTFCGSRKIHYIFCEMYVLLRLACSNTQVNHTVLIATGSFIFLTPLGFMIMSYVWIVKAILQIPSASSKYKAFSTCASHLAVVSLFYGTLCMVYLQPLHTYSMKDSVATVMYAVVTPMMNPFIYSLRNKDMHGALGRLLLGKVFQRLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LPR0</accession>
    <name>A0A8C4LPR0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Keratin, type II cytoskeletal 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cytokeratin-8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Keratin-8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Type-II keratin Kb8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">KRT8</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus matrix</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
            <location evidence="3">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14 15">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LPR0"/>
    <dbReference type="Ensembl" id="ENSEAST00005014413.2">
        <property type="protein sequence ID" value="ENSEASP00005013269.2"/>
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</entry>
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    <name>A0A8C4LQZ7_EQUAS</name>
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        <recommendedName>
            <fullName evidence="10">Zinc finger protein 750</fullName>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
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                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
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                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="P:establishment of skin barrier"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044091">
        <property type="term" value="P:membrane biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010719">
        <property type="term" value="P:negative regulation of epithelial to mesenchymal transition"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010628">
        <property type="term" value="P:positive regulation of gene expression"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039363">
        <property type="entry name" value="ZNF750"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039064">
        <property type="entry name" value="ZNF750_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14678">
        <property type="entry name" value="PROLINE-RICH PROTEIN 35-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14678:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 750"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15269">
        <property type="entry name" value="zf-C2H2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger protein 750-like zinc finger" evidence="12">
        <location>
            <begin position="6"/>
            <end position="57"/>
        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="478"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="560"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="581"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="597"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="647"/>
            <end position="667"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00040216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF15269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005012313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="712" mass="76802" checksum="621C2CCF162EAADF" modified="2022-01-19" version="1">MSLLKERKPKKPHYIPRPPGKPFKYKCFQCPFTCNEKSHLFNHMKYGLCKNSITLVSEQDRVPKCPKSNSLDSKQTSQLDPVAKPTSSKSVTSGLSHFDAKLQHSKDAKENVDLHTCGSHTCPGQKPALLKEAVPLSPEATMGTQPALEGVVRPSAFVPVGEQRLKGPEHVEPPAVLPLPTPSTKATSFHTKSAFHAPGYPWRAGSPFLPPEFPHKVPPTKGFGAVSPYMHPTIPDYPPHFYTEHGLATVYSPYLLAGNSPECDATLLAVYGAQDHRRFLPHPGPIPKHLSPSPSTYDHYRFFQQHHSNLPIPYGFYRPESAFSSYGLRLPPVAGISQDQSSHLLEEAALVYPASSPSKLNPPNSHRKHTEFEKESPTPEAKDPSKDGQRDTEGTKMSPRAGSAATGSPGRPSPTNFTQTSQPCAGLCSLSDKLASSSLGRLHQPEQSLAAFKPIKRSPERPHSQAPENRDEAPKSLNAVNTDATAQTGSACHGLEAAPCSPEDGSRMAPLNLSKKPETRPAAAHDPVYEDFTELQDMPLNLSVKDPCNALTPRPSFHSPPQEAEPATAAPKTEPESSGDGPDHTETKRNSLDSDEAPSTSPTGKAQGSRGAADSSDEQKQTAAVALCQLAAYSPGNVRVGDGEPTAQGSACQQRSPTLSATGSQEAQCDLRPKGQKRTSPRDAGKSQQGTKKTKPSDTARVFTLRKRTRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LT34</accession>
    <name>A0A8C4LT34_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Solute carrier family 35 member E4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SLC35E4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Putative transporter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TPT transporter family. SLC35E subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014690140.2">
        <property type="nucleotide sequence ID" value="XM_014834654.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LT34"/>
    <dbReference type="Ensembl" id="ENSEAST00005016088.2">
        <property type="protein sequence ID" value="ENSEASP00005014794.1"/>
        <property type="gene ID" value="ENSEASG00005010325.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106827025"/>
    <dbReference type="KEGG" id="eai:106827025"/>
    <dbReference type="CTD" id="339665"/>
    <dbReference type="GeneTree" id="ENSGT00730000111291"/>
    <dbReference type="OMA" id="MAGLNKW"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004853">
        <property type="entry name" value="Sugar_P_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050186">
        <property type="entry name" value="TPT_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11132">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03151">
        <property type="entry name" value="TPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="76"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="254"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="310"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Sugar phosphate transporter" evidence="8">
        <location>
            <begin position="48"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00093767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00093775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="350" mass="36996" checksum="18F50364959323AB" modified="2022-01-19" version="1">MCRCWLEHHDGRMTSAEAVAVADNARVAGSPEWPLDAPQALGQPGRARVAMAALVWLLAGASMSSLNKWIFTVHGFGQPLLLSALHMLAAALACHWGAQRPMPGRARRQVLLLSLTFGTSMACGNVGLSAVPLDLAQLATTTTPLITLALSALLLGRRHHPLQFAAMGPLCLGAACSLAGELRTPPAGCGFLLAATCLRGLKSIQQSALLQEERLDAVTLLYATSLPSFCLLAGAALVLEAGVAPPPAPTDSRLWACILLSCLLSVLYNLASFSLLALTSALTVHVLGNLTVVGNLVLSRLLFGSRLSTLSYVGIALTLSGMFLYHNCEFVASWAVRRGFWRRDQPGKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LWV9</accession>
    <name>A0A8C4LWV9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Tektin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">TEKT2</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia and flagellar axoneme. Forms filamentous polymers in the walls of ciliary and flagellar microtubules. Required for normal sperm mobility.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia and flagellar axoneme. Plays a key role in the assembly or attachment of the inner dynein arm to microtubules in sperm flagella and tracheal cilia. Forms filamentous polymers in the walls of ciliary and flagellar microtubules.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Microtubule inner protein component of sperm flagellar doublet microtubules. May interact with CCDC172.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="15">Cytoplasm</location>
            <location evidence="15">Cytoskeleton</location>
            <location evidence="15">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Flagellum axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the tektin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014695606.1">
        <property type="nucleotide sequence ID" value="XM_014840120.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LWV9"/>
    <dbReference type="Ensembl" id="ENSEAST00005019069.2">
        <property type="protein sequence ID" value="ENSEASP00005017562.1"/>
        <property type="gene ID" value="ENSEASG00005012098.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106830535"/>
    <dbReference type="KEGG" id="eai:106830535"/>
    <dbReference type="CTD" id="27285"/>
    <dbReference type="GeneTree" id="ENSGT00950000182894"/>
    <dbReference type="OMA" id="FDHRGKM"/>
    <dbReference type="OrthoDB" id="440745at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160111">
        <property type="term" value="C:axonemal A tubule inner sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036159">
        <property type="term" value="P:inner dynein arm assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048256">
        <property type="entry name" value="Tektin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000435">
        <property type="entry name" value="Tektins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19960">
        <property type="entry name" value="TEKTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19960:SF29">
        <property type="entry name" value="TEKTIN-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03148">
        <property type="entry name" value="Tektin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00511">
        <property type="entry name" value="TEKTIN"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="10 15" id="KW-0969">Cilium</keyword>
    <keyword evidence="9 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="8 15" id="KW-0282">Flagellum</keyword>
    <keyword evidence="6" id="KW-0493">Microtubule</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0832">Ubl conjugation</keyword>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="276"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="342"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00046707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU367040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005017562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="430" mass="49917" checksum="755084875768931C" modified="2022-01-19" version="1">MATLSVKPSQRYRLPDWHTNSYLLSTNAERQRDASHQIRQEARVLRNETTNQTIWDEHDNRTRLAERIDTVNRWKEMLDKCLMDLDAEIDALTQMRESAEQSLQAKNLPLDVAIECLTLRDSRRDIDVVKDPVEEELHKEVEVIDATKKALQQKISQAFEKLCLLQEVRQQLNSDHRGKMETLDIDRGCLSLNLKSPNISLKVDPTRVPNGSSTLKQWDDFSQFNKNRAEAEMKGAIELREAIVLTIAETNNQLEAQRVATEFAFRKRLREMEKVYSELKWQEKNTLEEIAELQEDIRHLEEDLRRKLLNLKLCHTRLESRTYRPNVELCRDQAQYGLTDEVHQLEATIAALKQKLAQAQDALDALYKHLARLQADIACKANSMLLDTKCMDTRRKLTVPAEKFVPEVDTFTRTTNRTLSPLKSCQLELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LYT8</accession>
    <name>A0A8C4LYT8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Charged multivesicular body protein 4A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">CHMP4A</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Late endosome membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LYT8"/>
    <dbReference type="Ensembl" id="ENSEAST00005018781.2">
        <property type="protein sequence ID" value="ENSEASP00005017285.2"/>
        <property type="gene ID" value="ENSEASG00005011974.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163323"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031902">
        <property type="term" value="C:late endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1060:FF:000001">
        <property type="entry name" value="Charged multivesicular body protein 4b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF14">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="93"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="301"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005017285.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="352" mass="38825" checksum="327371B098077953" modified="2023-09-13" version="2">MIPLCSPHPHPSPELCQEHEELFHWPQVVGCVTLHISLQETSTLFWGGGRPFYIVENEASALREARGSGAGGLGHVIEMGSSAPLDDVVAVPQRGIGRGQSPRDETGPSPPRARRKCAGRRGAQAGEAGVAMSGLGRLFGRGKKEKGPTPEEAIQKLKETEKILIKKQEFLEHKIQQELQTAKKHGTKNKRAALQALRRKKRLEQQLAQIDGTLSTLEFQREAIENATTNVEVLRTMELAAQGMKKAYQDMDIDKVDELMADITEQQEVAQQISDAISRPLGFGEDVDEDELLEELEQLEQEESARELLPVDDKEEEPPVKLPSVPTHVPAEPAPEADEDAEALKQLATWVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M0Z4</accession>
    <name>A0A8C4M0Z4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Olfactomedin like 2B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">OLFML2B</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014696629.2">
        <property type="nucleotide sequence ID" value="XM_014841143.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M0Z4"/>
    <dbReference type="Ensembl" id="ENSEAST00005021657.2">
        <property type="protein sequence ID" value="ENSEASP00005019943.2"/>
        <property type="gene ID" value="ENSEASG00005013764.2"/>
    </dbReference>
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    <dbReference type="KEGG" id="eai:106831123"/>
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</entry>
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    <accession>A0A8C4M124</accession>
    <name>A0A8C4M124_EQUAS</name>
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        <recommendedName>
            <fullName evidence="11">Left-right determination factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">LOC106844732</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <location evidence="1 11">Secreted</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2 11 12">Belongs to the TGF-beta family.</text>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Left-right determination factor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.90.10">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029034">
        <property type="entry name" value="Cystine-knot_cytokine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003942">
        <property type="entry name" value="LRDF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001839">
        <property type="entry name" value="TGF-b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001111">
        <property type="entry name" value="TGF-b_propeptide"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015615">
        <property type="entry name" value="TGF-beta-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017948">
        <property type="entry name" value="TGFb_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848:SF226">
        <property type="entry name" value="LEFT-RIGHT DETERMINATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11848">
        <property type="entry name" value="TGF-BETA FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00019">
        <property type="entry name" value="TGF_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00688">
        <property type="entry name" value="TGFb_propeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037402">
        <property type="entry name" value="TGFb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01427">
        <property type="entry name" value="TGFBETA4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00204">
        <property type="entry name" value="TGFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57501">
        <property type="entry name" value="Cystine-knot cytokines"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00250">
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    <dbReference type="PROSITE" id="PS51362">
        <property type="entry name" value="TGF_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="3 11" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11 12" id="KW-0339">Growth factor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="7 13" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040419215" description="Left-right determination factor" evidence="13">
        <location>
            <begin position="22"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="domain" description="TGF-beta family profile" evidence="14">
        <location>
            <begin position="243"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU000354"/>
        </source>
    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="367" mass="40774" checksum="BB2ABF008597C47C" modified="2023-09-13" version="2">MQPVWLCWALWALPLTGPGAALTEEQILGSLLQQLHLSEVPALDQGDVGQLVIPAHVRAQYVALLQRSHGTHSRGKRFSQNFREVAGRLLGSDASRHLLVFAMEQRLPPDRELLQAVLRLFQEPVPEAALRTHGRLSPRSHRARVTVEWLHVRDDGSNRTSLIDSRLVSVHESGWKAFDVTEAVNFWRQLGRPRQPLLLQVSVQREQPGPRAASAHRLVRFASQGPAGGGRGEPQLELHSLDLGRYGAQGDCDPEAPVTEGTRCCRREMYIDLEGLKWAENWVLEPPGFLAYECVGTCRQPPEPLSFKWPFLGPRQCIASETTSLPVIVSVKEGGRPRAQVVSLPDMRVQKCSCASDGAPVPRKLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M1D9</accession>
    <name>A0A8C4M1D9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zinc finger protein 283</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ZNF283</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014868299.3"/>
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    <dbReference type="RefSeq" id="XP_014723788.2">
        <property type="nucleotide sequence ID" value="XM_014868302.3"/>
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    <dbReference type="RefSeq" id="XP_044614896.1">
        <property type="nucleotide sequence ID" value="XM_044758961.2"/>
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    <dbReference type="RefSeq" id="XP_044614897.1">
        <property type="nucleotide sequence ID" value="XM_044758962.2"/>
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    <dbReference type="RefSeq" id="XP_044614898.1">
        <property type="nucleotide sequence ID" value="XM_044758963.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070354698.1">
        <property type="nucleotide sequence ID" value="XM_070498597.1"/>
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    <dbReference type="RefSeq" id="XP_070354699.1">
        <property type="nucleotide sequence ID" value="XM_070498598.1"/>
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    <dbReference type="RefSeq" id="XP_070354700.1">
        <property type="nucleotide sequence ID" value="XM_070498599.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070354701.1">
        <property type="nucleotide sequence ID" value="XM_070498600.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M1D9"/>
    <dbReference type="Ensembl" id="ENSEAST00005021459.2">
        <property type="protein sequence ID" value="ENSEASP00005019774.2"/>
        <property type="gene ID" value="ENSEASG00005013615.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106848429"/>
    <dbReference type="CTD" id="284349"/>
    <dbReference type="GeneTree" id="ENSGT00940000161267"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000053">
        <property type="entry name" value="zinc finger protein 182 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000295">
        <property type="entry name" value="zinc finger protein 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000204">
        <property type="entry name" value="Zinc finger protein 331"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000044">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000338">
        <property type="entry name" value="zinc finger protein 383"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001498">
        <property type="entry name" value="Zinc finger protein 404"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000773">
        <property type="entry name" value="Zinc finger protein 44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001402">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002254">
        <property type="entry name" value="Zinc finger protein 540"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000052">
        <property type="entry name" value="zinc finger protein 546 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000737">
        <property type="entry name" value="Zinc finger protein 565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000710">
        <property type="entry name" value="Zinc finger protein 768"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="zinc finger protein 90 homolog"/>
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        <property type="entry name" value="zinc finger protein 90 homolog"/>
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        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
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    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24393:SF159">
        <property type="entry name" value="ZINC FINGER PROTEIN 345-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13912">
        <property type="entry name" value="zf-C2H2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 13" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="15">
        <location>
            <begin position="43"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="204"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="232"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="260"/>
            <end position="287"/>
        </location>
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        <location>
            <begin position="288"/>
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        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="316"/>
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        </location>
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    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="344"/>
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        </location>
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    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="372"/>
            <end position="399"/>
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    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="400"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="456"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="484"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="512"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="540"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="568"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="596"/>
            <end position="623"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00067219"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005019774.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="650" mass="74830" checksum="02F94ABC959755F9" modified="2023-09-13" version="2">MSYSSSMFDSCSDFSGFCASPVEEPHGALTSSYDSRTMTDALVTFSDVAIGFSQEEWECLDPAQRDLYMDVMLENYSNVASLDLESTDETKNTFSEKDNSEIKFSQWEIKERSKTLGLEASIFKNNWNCESKFEGLQGPQEGYFSQMIISYEEMPTYRESESLTAHQRIHSQEEPYVCKEFGEACSPDSKLGEDERTQMAEEHFECKECGKDFLNAYHLTVHQRFHTGEKPYECKECGKTFSWGSSLIKHVRIHTGEKPFACKECGRTFSRGYHLTEHQKIHIGVKSYECKECGKAFFWGSSLAKHEIIHTGEKPYKCKECGKAFSRGYQLTQHQKIHTGRKPYECKVCGKAFRWGYQLTRHQIFHTGEKAYECKECGKNFNCGSSLLQHERIHTGEKPYECKDCGKAFSRGYHLTEHQKIHTGVKPFECKECGKAFTWGSSLVKHERVHTGVKSHECKECGKAFGSGYQLTRHQRFHTGEKPYECKACGKAFNCASSLVKHERIHTGEKPYGCKECGKAFIRGYHLTQHQRIHTGEKPFKCKECGKAFRWGSSLVKHERIHTGEKSYECKECGQAFDNGYQLSFHQIFHADEKPYQCKEFGNTFPHGSSLVQHERTHSNDEPYKYEEWGEAFMWTPYSNETVDTGETYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M2L4</accession>
    <name>A0A8C4M2L4_EQUAS</name>
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        <recommendedName>
            <fullName evidence="8">Actin-like protein 7B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">LOC106830712</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2 4">Belongs to the actin family.</text>
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        <property type="nucleotide sequence ID" value="XM_014840457.3"/>
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        <property type="component" value="Chromosome 21"/>
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        <property type="term" value="C:cytoskeleton"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="entry name" value="Actin like 7B"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Actin, alpha skeletal muscle"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Actin, Chain A, domain 4"/>
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        <property type="entry name" value="Actin"/>
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        <property type="entry name" value="Actin_CS"/>
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        <property type="entry name" value="ATPase_NBD"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Actin"/>
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        <property type="entry name" value="ACTIN"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="ACTINS_2"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0206">Cytoskeleton</keyword>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
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        <name evidence="10" type="primary">EIF4G3</name>
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        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:positive regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="entry name" value="Putative eukaryotic translation initiation factor 4 gamma 1"/>
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        <property type="entry name" value="Initiation_fac_eIF4g_MI"/>
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        <property type="entry name" value="MIF4G-like_typ-3"/>
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        <property type="entry name" value="W2_domain"/>
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    <dbReference type="PANTHER" id="PTHR23253">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMA 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="W2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="eIF5C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00544">
        <property type="entry name" value="MA3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="3"/>
    </dbReference>
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        <property type="entry name" value="MI"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0810">Translation regulation</keyword>
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            <end position="1532"/>
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    <feature type="domain" description="W2" evidence="8">
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            <begin position="1605"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00005775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005020864.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1774" mass="194946" checksum="635D950CBC38B677" modified="2023-09-13" version="2">VKKIRRHRLSPATEKGAASPGEGDKDPPPPAHEDIAVKGSAAAAGTGPGTGAAAAAAAVPPPHPAAAAAAAAAAAAAAAAAAAAPPHPNIRALQTPAPQQIPRGPVQQPLEDRIFTPTVSAVYSTVTQVARQPGPPTPSPYSAHEINKGHPNLAATPPGHASSPGLSQTPYPSGQNAGPTTLVYPQAPQTMNSQPQTRSPFFQRPQIQPPRATIPNSSPSIRPGAQTPTAVYQANQHIMMVNHLPMPYPVPQGPQYCIPQYRHSGPPYVGPPQQYPVQPPGPGPFYPGPGPGDFPNAYGTPFYPSQPVYQSAPIIVPTQQQPPPAKREKKTIRIRDPNQGGKDITEEIMSGGGSRNPTPPIGRPTSTPTPPQLPSQVPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQAGQTSETTAVESIPELPLPPSPTTVSPLARSTIASPTSAALSSQPILTTAIDDRCELYSSKEDTIPVPSPASCTETSDPSPTDIIDDDICKKSCSVAPNDSPLIVSTNLINEMNGVSEKLPATESIVEIVKQEVLPLTLELEILQSPPEEMKVECVPAPITRSTVPSFSPSPPTPPVSPPLTAVIIAAATTNVNTASAPTAVQRVLEEDENIRTCLSEDAPEIQNKIETEADEQTEEIVDSQNLSSRKSPVPAQTAITAPKMWKKPKDRMRTTEEVLEAESEPKAEEELSGDKVLDSEQDKMSQGFHPERDPSDLKKVKAGEENGEEAEPVRNGAESVSEGEGIDANSGSTDSSGDGVPFPFKPESWKPADTEGKKQYDREFLLDFQFMPACIQKPEGLPPISDVVLDKINQPKLPMRTLDPRILPRGPDFTPAFADFGRQTPGGRGVPLLNVGPRRSQPSQRREPRKIITVPVKEDVHLKKAENAWKPSQKRDIQAEDPENIKTQELFRKVRSILNKLTPQMFNQLMKQVSGLTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLVTLKVPMADKPGNTVNFRKLLLNRCQKEFEKDKADDDVFEKKQKELEAASAPEERTRLHDELEEAKDRARRRSIGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLCNWVSRRADQGPKTIEQIHKEAKIEEQEEQRKVQQLMTKEKRRPGVQRVDEGGWNTVQGAKNSRVLDPSKFLKITKPTIDEKIQLVPKAQLGSWGKGSSGGAKASETDALRSSASSLNRFSALQPPAPSGSASSTPLEFDSRRTLTSRGSMGREKNDKPLPSATTRPNTFMRGSSSKDLLDNQSQEEQRREMLETVKQLTGGMDVERSSTEADRSKTRESAKSEIPATSASDKPTLSEEEMERKSKSIIDEFLHINDFKEAMQCVEELNAQGLLHVFVRMGVESTLERSQITRDHMGQLLYQLVQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYLAELVTPMLKEGGISMRELIIEFSKPLLPVGRAGILLSEILHLLCKQMSHKKVGAVWREADLSWKDFLPEGEDVHSFLLEQKLDFIESDSSCSSEALSKKELSAEELYKRLEKLIIEDKANDEQIFDWVEANLDESQMSSPTFLRALMTAVCKAAIIADSSAFRVDTAVIKQRVPILLKYLDSDTEKELQALYALQASIVKLDQPANLLRMFFDCLYDEEVISEDAFYKWESSKDPAEQNGKGVALKSVTAFFTWLREAEEESEDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M833</accession>
    <name>A0A8C4M833_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Taste receptor type 2</fullName>
        </recommendedName>
    </protein>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the G-protein coupled receptor T2R family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4M833"/>
    <dbReference type="Ensembl" id="ENSEAST00005023314.2">
        <property type="protein sequence ID" value="ENSEASP00005021478.2"/>
        <property type="gene ID" value="ENSEASG00005014701.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01150000286975"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033038">
        <property type="term" value="F:bitter taste receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15027">
        <property type="entry name" value="7tm_TAS2R43-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000042">
        <property type="entry name" value="Taste receptor type 2 member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007960">
        <property type="entry name" value="TAS2R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11394">
        <property type="entry name" value="TASTE RECEPTOR TYPE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11394:SF27">
        <property type="entry name" value="TASTE RECEPTOR TYPE 2 MEMBER 20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05296">
        <property type="entry name" value="TAS2R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="8 12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="3 12" id="KW-0919">Taste</keyword>
    <keyword evidence="10 12" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="46"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="84"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="116"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="167"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="215"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005021478.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="298" mass="34511" checksum="4C38B88A474D62B8" modified="2023-09-13" version="2">MITLLPIIFSILKMTEFILGNFANGFIALVNSDQILMALSISRTGLLWLMLINWHATVLTPVLYSLEVRMIVRIAWAVSNHFSMWLAASLSIFYLLKIANFSSCIFLYLKWRVKSVLLGILLGTSVFLVSYLAVLCIDENTQTNEYEGKITWKTKLRHTVYLSDMTLFMLINFVPFVMSLTCFLLLIISLWKHLRKMQLNDKGSQDPSTKVHQRAMQTVVSFLFLLSCYFLTLVISVWNSNRLQNEWLLMLCQALGMLHPSSHSFILIWGNKKLRQALKNVRKIVCRFYCLNLQSFGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M8Y4</accession>
    <name>A0A8C4M8Y4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="20">Integrin subunit alpha L</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ITGAL</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 17">Membrane</location>
            <topology evidence="1 17">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17">Belongs to the integrin alpha chain family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014697888.1">
        <property type="nucleotide sequence ID" value="XM_014842402.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M8Y4"/>
    <dbReference type="Ensembl" id="ENSEAST00005020721.2">
        <property type="protein sequence ID" value="ENSEASP00005019087.2"/>
        <property type="gene ID" value="ENSEASG00005013044.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106831918"/>
    <dbReference type="KEGG" id="eai:106831918"/>
    <dbReference type="CTD" id="3683"/>
    <dbReference type="GeneTree" id="ENSGT00940000161495"/>
    <dbReference type="OrthoDB" id="5317514at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 14"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034687">
        <property type="term" value="C:integrin alphaL-beta2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030369">
        <property type="term" value="F:ICAM-3 receptor activity"/>
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        <property type="entry name" value="Alpha L integrin"/>
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        <property type="entry name" value="Int_alpha_beta-p"/>
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    <dbReference type="InterPro" id="IPR000413">
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    <dbReference type="InterPro" id="IPR013649">
        <property type="entry name" value="Integrin_alpha_Ig-like_1"/>
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</entry>
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            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="Ensembl"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:membrane hyperpolarization"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048240">
        <property type="term" value="P:sperm capacitation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd07042">
        <property type="entry name" value="STAS_SulP_like_sulfate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.24">
        <property type="entry name" value="STAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018045">
        <property type="entry name" value="S04_transporter_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011547">
        <property type="entry name" value="SLC26A/SulP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001902">
        <property type="entry name" value="SLC26A/SulP_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002645">
        <property type="entry name" value="STAS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036513">
        <property type="entry name" value="STAS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00815">
        <property type="entry name" value="sulP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11814">
        <property type="entry name" value="SULFATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01740">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00916">
        <property type="entry name" value="Sulfate_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52091">
        <property type="entry name" value="SpoIIaa-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01130">
        <property type="entry name" value="SLC26A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50801">
        <property type="entry name" value="STAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="338"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="369"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="406"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="464"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="domain" description="STAS" evidence="6">
        <location>
            <begin position="519"/>
            <end position="714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025677.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="758" mass="84009" checksum="75FD04234B35EBB2" modified="2023-09-13" version="2">MIEPFGNQYVVARRVYSANDFGEEHKKKERRHKTPLDHLKQCCSCSTKKAKRIALSLFPIASWLPAYQIKEWLLSDIVSGISTGLVAVLQGLAFALLVNVPPSYGLYAAFFPVIIYFFLGTSRHISVGPFPVLSMMVGAVVVRVTSKPVQGGNNTSISNDLDSSSDEQKVMVAAMVTILSGIIQLLLGVLQIGFVVIYLSESLISGFTTAAAIHVLVSQLKFILQLTVPAHTDPFSIFKVLESVFTQIQKTNIADLVTALIILVVVSAVKEVNQRFKAKLPVPIPIELIMTVIAAGVSYGFDFKTRFNVAVVGKMNSGFQPPIQPDPKIFQDTIGDSFGIAIVGFAVAFSVARVYSLKYDYPINGNQELIALGLGNIFGGSFRGFASSTALSRSGVQESTGGKTQIAGILSAVIVLIVIVAIGFLLEPLQKSVLAALALGNLKGMLMQFAEIGRLWRKDKYDCLIWIMTFIFAIVLGLGLGLAASVAFQLLTIVFRTQFPKCSTLANVGRSNIYKNRKDYSDMYEPEGIKIFRCPSPIYFANIGFFRQKLFDAVGFSPLRILRKRKKALKKIRKMQKKGLIQVTPKGCICTVDNFKDSDEELDNHEIEELDQPINTTDLPFQIDWNADLPLNIVVPKISLHSLILDFSAVSFLDVSSMRGLKKIFQEFIRIEVDVYIVGTDDDFIEKLTRCEFFDDEVKDSIFFLTIHDAVLHILMKKDYITSKVSTSQEKEAKTDFIVNTNGGLRNRECQVPLETKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MFV9</accession>
    <name>A0A8C4MFV9_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">RCC1 and BTB domain containing protein 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">RCBTB2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_014694407.1">
        <property type="nucleotide sequence ID" value="XM_014838921.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014694408.1">
        <property type="nucleotide sequence ID" value="XM_014838922.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014694409.1">
        <property type="nucleotide sequence ID" value="XM_014838923.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014694410.1">
        <property type="nucleotide sequence ID" value="XM_014838924.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014694411.1">
        <property type="nucleotide sequence ID" value="XM_014838925.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044633098.1">
        <property type="nucleotide sequence ID" value="XM_044777163.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070376760.1">
        <property type="nucleotide sequence ID" value="XM_070520659.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070376762.1">
        <property type="nucleotide sequence ID" value="XM_070520661.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MFV9"/>
    <dbReference type="Ensembl" id="ENSEAST00005027516.2">
        <property type="protein sequence ID" value="ENSEASP00005025341.2"/>
        <property type="gene ID" value="ENSEASG00005017255.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106829684"/>
    <dbReference type="KEGG" id="eai:106829684"/>
    <dbReference type="CTD" id="1102"/>
    <dbReference type="GeneTree" id="ENSGT00940000158925"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="CDD" id="cd18529">
        <property type="entry name" value="BACK_RCBTB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18354">
        <property type="entry name" value="BTB_POZ_RCBTB2_CHC1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000033">
        <property type="entry name" value="RCC1 and BTB domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000038">
        <property type="entry name" value="RCC1 and BTB domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000040">
        <property type="entry name" value="RCC1 and BTB domain-containing protein 2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.420">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058923">
        <property type="entry name" value="RCC1-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051625">
        <property type="entry name" value="Signaling_Regulatory_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872">
        <property type="entry name" value="BTK-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872:SF3">
        <property type="entry name" value="RCC1 AND BTB DOMAIN CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00651">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25390">
        <property type="entry name" value="WD40_RLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00633">
        <property type="entry name" value="RCCNDNSATION"/>
    </dbReference>
    <dbReference type="SMART" id="SM00225">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00626">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="118"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="171"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="224"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="2">
        <location>
            <begin position="276"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="3">
        <location>
            <begin position="394"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005025341.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="551" mass="60281" checksum="F946C6EF7959BE98" modified="2023-09-13" version="1">MEEELPLFCGESGKPVQATLSSLKMLDVGKWPIFSLCSEEELQLIRQACVFGSAGNEVLYTTVNDEIFVLGTNCCGCLGLGDVQSTIEPRRLDSLTGKKVACLSYGSGPHVVLATSEGEVFTWGHNAYSQLGNGTTNHGLVPCHISTNLSNKRVIEVACGSYHSLVLTSDGEVFAWGYNNSGQVGSGSTANQPIPRRVTGCLQNKVVVNIACGQMCSMAVVDTGEVYVWGYSGNGQLGLGGSGNQPTPCRVAALQGIRVQRAACGYAHTLVLTDEGQVYVWGANSYGQLGTGNKSNQPYPTPIIVEKDRIIEIAACHSTHTSAAKTQGGHVYMWGQCRGQSVILPHLTHFSCTDDVFACFASPAVSWRLLTVEPDDHLTVAESLKREFDNPNTADLKFLVDGKYIYAHKVLLKIRCEHFRSSLEDHEDDIVEMSEFSYPVYRAFLEYLYTDSISLSPEEAVGLLDLATFYRENRLKKLCQQTIKQGICEENAIALLSAAVKYDAQDLEEFCFRFCINHLTMVTQTAGFAEMDHDLMKNFISKASRVGAFKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MHI1</accession>
    <name>A0A8C4MHI1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Probable ATP-dependent RNA helicase DDX56</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">DEAD box protein 56</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">DDX56</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Nucleolar RNA helicase that plays a role in various biological processes including innate immunity, ribosome biogenesis or nucleolus organization. Plays an essential role in maintaining nucleolar integrity in planarian stem cells. Maintains embryonic stem cells proliferation by conventional regulation of ribosome assembly and interaction with OCT4 and POU5F1 complex. Regulates antiviral innate immunity by inhibiting the virus-triggered signaling nuclear translocation of IRF3. Mechanistically, acts by disrupting the interaction between IRF3 and importin IPO5. May play a role in later stages of the processing of the pre-ribosomal particles leading to mature 60S ribosomal subunits. Has intrinsic ATPase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 24">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">May form homooligomeric complexes. Interacts with IRF3. Interacts with OCT4 and POU5F1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DEAD box helicase family. DDX56/DBP9 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="RefSeq" id="XP_014683870.1">
        <property type="nucleotide sequence ID" value="XM_014828384.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MHI1"/>
    <dbReference type="Ensembl" id="ENSEAST00005028319.2">
        <property type="protein sequence ID" value="ENSEASP00005026084.1"/>
        <property type="gene ID" value="ENSEASG00005017795.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822950"/>
    <dbReference type="KEGG" id="eai:106822950"/>
    <dbReference type="CTD" id="54606"/>
    <dbReference type="GeneTree" id="ENSGT00550000074946"/>
    <dbReference type="OMA" id="NASEQCV"/>
    <dbReference type="OrthoDB" id="1191041at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17961">
        <property type="entry name" value="DEADc_DDX56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000939">
        <property type="entry name" value="Probable ATP-dependent RNA helicase DDX56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001046">
        <property type="entry name" value="Probable ATP-dependent RNA helicase ddx56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050079">
        <property type="entry name" value="DEAD_box_RNA_helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE RHLE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47959:SF21">
        <property type="entry name" value="DEAD-BOX HELICASE 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="21">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="19">
        <location>
            <begin position="38"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="20">
        <location>
            <begin position="230"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="503"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="17">
        <location>
            <begin position="7"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="505"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="531"/>
            <end position="546"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00066108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00075547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="546" mass="61311" checksum="4AA47F670E664005" modified="2022-01-19" version="1">MAEPEALGFEHMGLDPRLLQAVTDLGWSRPTLIQEKAIPLALEGKDLLARARTGSGKTAAYAIPMLQLLLHRKATGPAVEQAVRGLVLVPTKELARQAQSMIQQLAAYCARDIRVANVSAAEDSASQRAVLMEKPDVVVGTPSRILNHLQQDSLKLRDSLELLVVDEADLLFSFGFEEELKSLLCHLPRIYQAFLMSATFNEDVQALKELVLHNPVTLKLQESQLPGPEQLQQFQVVCETEEDKFLLLYALLKLSLIRGKSLLFVNTLERSYRLRLFLEQFSIPTCVLNGELPLRSRCHIISQFNQGFYDCVIATDAEVLGAPVKGKRQGKGPKGDRASDPEAGVARGIDFHHVCAVLNFDLPPTPEAYIHRAGRTARANNPGIVLTFVLPTEQSHLGKIEELLGGESTAPVLLPYQFRMEEIEGFRYRCRDAMRSVTKQSIREARLKEIKEELLHSEKLKTYFEDNPRDLQLLRHDLPLHPAVVKPHLGHVPDYLVPSALRGLVHPRKKRKKPSSSKKAKKAKTQNPLRSFKHRREKRRPTAMPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MI18</accession>
    <name>A0A8C4MI18_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Draxin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Dorsal inhibitory axon guidance protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Dorsal repulsive axon guidance protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">DRAXIN</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Chemorepulsive axon guidance protein required for the development of spinal cord and forebrain commissures. Acts as a chemorepulsive guidance protein for commissural axons during development. Able to inhibit or repel neurite outgrowth from dorsal spinal cord. Inhibits the stabilization of cytosolic beta-catenin (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist of Wnt signaling pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with LRP6.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the draxin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044625611.1">
        <property type="nucleotide sequence ID" value="XM_044769676.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MI18"/>
    <dbReference type="Ensembl" id="ENSEAST00005028297.2">
        <property type="protein sequence ID" value="ENSEASP00005026063.1"/>
        <property type="gene ID" value="ENSEASG00005017783.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106836670"/>
    <dbReference type="KEGG" id="eai:106836670"/>
    <dbReference type="CTD" id="374946"/>
    <dbReference type="GeneTree" id="ENSGT00390000013828"/>
    <dbReference type="OMA" id="WTPQTSH"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021960">
        <property type="term" value="P:anterior commissure morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021528">
        <property type="term" value="P:commissural neuron differentiation in spinal cord"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021516">
        <property type="term" value="P:dorsal spinal cord development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110088">
        <property type="term" value="P:hippocampal neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030517">
        <property type="term" value="P:negative regulation of axon extension"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090090">
        <property type="term" value="P:negative regulation of canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110091">
        <property type="term" value="P:negative regulation of hippocampal neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016055">
        <property type="term" value="P:Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03060">
        <property type="entry name" value="Draxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029094">
        <property type="entry name" value="Draxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28610">
        <property type="entry name" value="DRAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28610:SF1">
        <property type="entry name" value="DRAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15550">
        <property type="entry name" value="Draxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0964">Secreted</keyword>
    <keyword evidence="3 5" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0879">Wnt signaling pathway</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5035016666" description="Draxin" evidence="5">
        <location>
            <begin position="28"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="38"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="115"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="132"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="348" mass="38239" checksum="B17636839CBA7FCF" modified="2022-01-19" version="1" precursor="true">MAGLPAHVTSMLFLALLLLLELSLVDSLGPEAPARNLPENHIDLPGPALWTPQASHHRRRGLGKKERGPGMPGLAQDGAVVTATRQASRLPGAGGLLPGQRPAGLLQGKDLLLGLALPDPEKDNRSPGSERTKKRGREHKRRRERLKLHRGRALVRGPSSLMKKAEPAEDQAPDAAMEESSTSLAPTILYLTTFEAAPATEESLILPVTSLWPQAQPRPDGEVMPTLDMALFDWTDYEDLKPEVWPSAKKKEKHRGKLSIDGNETSPAEGEPCDHHQDCLPGTCCDLREHLCTPHNRGLNNKCFDDCMCSEGLRCYAKFHRNRRVTRRKGRCVEPETANGDQGSFINV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MI94</accession>
    <name>A0A8C4MI94_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FERM domain-containing protein 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">FRMD5</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">May be involved in regulation of cell migration. May regulate cell-matrix interactions via its interaction with ITGB5 and modifying ITGB5 cytoplasmic tail interactions such as with FERMT2 and TLN1. May regulate ROCK1 kinase activity possibly involved in regulation of actin stress fiber formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell junction</location>
            <location evidence="1">Adherens junction</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014702574.2">
        <property type="nucleotide sequence ID" value="XM_014847088.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MI94"/>
    <dbReference type="Ensembl" id="ENSEAST00005028707.2">
        <property type="protein sequence ID" value="ENSEASP00005026434.2"/>
        <property type="gene ID" value="ENSEASG00005027709.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106835008"/>
    <dbReference type="CTD" id="84978"/>
    <dbReference type="GeneTree" id="ENSGT00940000156346"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005912">
        <property type="term" value="C:adherens junction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031032">
        <property type="term" value="P:actomyosin structure organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13192">
        <property type="entry name" value="FERM_C_FRMD3_FRMD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17102">
        <property type="entry name" value="FERM_F1_FRMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000458">
        <property type="entry name" value="Erythrocyte membrane protein band 4.1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000043">
        <property type="entry name" value="FERM domain-containing protein 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000006">
        <property type="entry name" value="FERM domain-containing protein 5 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280">
        <property type="entry name" value="4.1 G PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23280:SF5">
        <property type="entry name" value="FERM DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08736">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00373">
        <property type="entry name" value="FERM_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09379">
        <property type="entry name" value="FERM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00935">
        <property type="entry name" value="BAND41"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00661">
        <property type="entry name" value="ERMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00295">
        <property type="entry name" value="B41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01195">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01196">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47031">
        <property type="entry name" value="Second domain of FERM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00660">
        <property type="entry name" value="FERM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50057">
        <property type="entry name" value="FERM_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0965">Cell junction</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FERM" evidence="7">
        <location>
            <begin position="17"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="344"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="385"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="458"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="388"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00054411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00067909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005026434.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="512" mass="57722" checksum="9AE418DC5EF93215" modified="2023-09-13" version="2">MLSRLMSGSSRSLEREYSCTVRLLDDSEYTCTIQRDAKGQYLFDLLCHHLNLLEKDYFGIRFVDPDKQRHWLEFTKSVVKQLRSQPPFTMCFRVKFYPADPAALKEEITRYLVFLQIKRDLYHGRLLCKTSDAALLAAYILQAEIGDYDPGKHPEGYSSKFQFFPKHSEKLERKIAEIHKTELSGQTPATSELNFLRKAQTLETYGVDPHPCKDVSGNAAFLAFTPFGFVVLQGNKRVHFIKWNEVTKLKFEGKTFYLYVSQKEEKKIILTYFAPTPEACKHLWKCGIENQAFYKLEKSSQVRTVSSSNLFFKGSRFRYSGRVAKEVMESSAKIKREPPEIHRAGMVPSRSCPSITHGPRLSSVPRTRRRAVHISIMEGLESLRDSAHSTPVRSSSHGDTFLPHVRSSRADSNERVAVIADEAYSPADSVLPTPVAEHSLELMLLSRQINGATCSIEEEKESEASTPTAAEVEALGGELRALCQGHSRPEEEQSGHLKGPPLQQAAAAGVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MIB7</accession>
    <name>A0A8C4MIB7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribosome-releasing factor 2, mitochondrial</fullName>
            <shortName evidence="7">RRF2mt</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Elongation factor G 2, mitochondrial</fullName>
            <shortName evidence="7">EF-G2mt</shortName>
            <shortName evidence="7">mEF-G 2</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">GFM2</name>
        <name evidence="7" type="synonym">EFG2</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Mitochondrial GTPase that mediates the disassembly of ribosomes from messenger RNA at the termination of mitochondrial protein biosynthesis. Acts in collaboration with MRRF. GTP hydrolysis follows the ribosome disassembly and probably occurs on the ribosome large subunit. Not involved in the GTP-dependent ribosomal translocation step during translation elongation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MIB7"/>
    <dbReference type="Ensembl" id="ENSEAST00005029341.2">
        <property type="protein sequence ID" value="ENSEASP00005027031.2"/>
        <property type="gene ID" value="ENSEASG00005018285.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106824016"/>
    <dbReference type="CTD" id="84340"/>
    <dbReference type="GeneTree" id="ENSGT00550000074890"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01886">
        <property type="entry name" value="EF-G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16262">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03713">
        <property type="entry name" value="EFG_mtEFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04092">
        <property type="entry name" value="mtEFG2_II_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01693">
        <property type="entry name" value="mtEFG2_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000053">
        <property type="entry name" value="Ribosome-releasing factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000033">
        <property type="entry name" value="Ribosome-releasing factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000008">
        <property type="entry name" value="Ribosome-releasing factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000005">
        <property type="entry name" value="Ribosome-releasing factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000514">
        <property type="entry name" value="Ribosome-releasing factor 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03059">
        <property type="entry name" value="mEF_G_2"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR053905">
        <property type="entry name" value="EF-G-like_DII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030851">
        <property type="entry name" value="EFG2"/>
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    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009022">
        <property type="entry name" value="EFG_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035649">
        <property type="entry name" value="EFG_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
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    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43261:SF1">
        <property type="entry name" value="RIBOSOME-RELEASING FACTOR 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43261">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22042">
        <property type="entry name" value="EF-G_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="8">
        <location>
            <begin position="68"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="77"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="141"/>
            <end position="145"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="195"/>
            <end position="198"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005027031.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MJG6</accession>
    <name>A0A8C4MJG6_EQUAS</name>
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        <recommendedName>
            <fullName evidence="13">MAX gene-associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">MGA</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Functions as a dual-specificity transcription factor, regulating the expression of both MAX-network and T-box family target genes. Functions as a repressor or an activator. Binds to 5'-AATTTCACACCTAGGTGTGAAATT-3' core sequence and seems to regulate MYC-MAX target genes. Suppresses transcriptional activation by MYC and inhibits MYC-dependent cell transformation. Function activated by heterodimerization with MAX. This heterodimerization serves the dual function of both generating an E-box-binding heterodimer and simultaneously blocking interaction of a corepressor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014712821.2">
        <property type="nucleotide sequence ID" value="XM_014857335.3"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005025413.2">
        <property type="protein sequence ID" value="ENSEASP00005023418.2"/>
        <property type="gene ID" value="ENSEASG00005015686.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106841350"/>
    <dbReference type="CTD" id="23269"/>
    <dbReference type="GeneTree" id="ENSGT00940000156269"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071339">
        <property type="term" value="C:MLL1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001708">
        <property type="term" value="P:cell fate specification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045893">
        <property type="term" value="P:positive regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18911">
        <property type="entry name" value="bHLHzip_MGA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20195">
        <property type="entry name" value="T-box_MGA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.820:FF:000009">
        <property type="entry name" value="MAX gene-associated protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.280.10:FF:000040">
        <property type="entry name" value="MAX gene-associated protein isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.820">
        <property type="entry name" value="Transcription factor, T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037935">
        <property type="entry name" value="MAX_gene-associated_bHLHzip"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032060">
        <property type="entry name" value="MGA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046360">
        <property type="entry name" value="T-box_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036960">
        <property type="entry name" value="T-box_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001699">
        <property type="entry name" value="TF_T-box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018186">
        <property type="entry name" value="TF_T-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267:SF32">
        <property type="entry name" value="MAX GENE-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11267">
        <property type="entry name" value="T-BOX PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16059">
        <property type="entry name" value="MGA_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00907">
        <property type="entry name" value="T-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00937">
        <property type="entry name" value="TBOX"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00425">
        <property type="entry name" value="TBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01264">
        <property type="entry name" value="TBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50252">
        <property type="entry name" value="TBOX_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="11 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="T-box" evidence="16">
        <location>
            <begin position="79"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="domain" description="BHLH" evidence="17">
        <location>
            <begin position="2423"/>
            <end position="2474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="259"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="595"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="886"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="926"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="969"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1248"/>
            <end position="1330"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1378"/>
            <end position="1429"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1469"/>
            <end position="1515"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1895"/>
            <end position="1923"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1990"/>
            <end position="2012"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2116"/>
            <end position="2163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2279"/>
            <end position="2315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2530"/>
            <end position="2596"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2676"/>
            <end position="2711"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="2940"/>
            <end position="2967"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
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    </feature>
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        <location>
            <begin position="901"/>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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            <dbReference type="PROSITE-ProRule" id="PRU00201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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        </source>
    </evidence>
    <sequence length="3065" mass="335423" checksum="655060F45868FC14" modified="2023-09-13" version="2">MEEKQQIILANQDGGTVAGTAPTFFVILKQPGNGKTDQGILVTNRDACALASSVSSPGKSKGKICLPADCTVGGITVTLDNNSMWNEFYHRSTEMILTKQGRRMFPYCRYWITGLDSNMKYILVMDISPVDNHRYKWNGRWWEPSGKAEPHVLGRVFIHPESPSTGHYWMHQPVSFYKLKLTNNTLDQEGHIILHSMHRYLPRLHLVPAEKATEVIQLNGPGVHTFTFPQTEFFAVTAYQNIQITQLKIDYNPFAKGFRDDGLNSKPQRDGKQKNSSDQEGSSVPSSPGQRVRLTEGEGSDIQLTDSDALSRGHETSGKGLEKPSLNVKRDFLGFMDIDSALGEVPQLKQEVSESLVANNFEDGSHVASPLDPNGNFNVVIKEEPLDDYEYGLGECPEGVTVKQEETDEETDVYSNSDDDPILEKQLKRHNKVDNLEADHPSSKWLPNSPSGVAKAKMFKLDAGKMPVVYLEPCAVTKSTVKISELPDNMLSTSRKDKSSVLTELDYLPMYIENSNETGFCLGKESENGLRKHSPDLRVVQKYPSLKEPQWKYPDISDSFNTETILDSSKSSTGDSFLGKEDLGRKRATMLKISTASKAVNANQNSPLNVPGKRGRPRKLKLSKAGRPPKNTGKSLTSTKNTPLGPGSTFPDVKPDLEDVDGVLFVSFESKEALDIHAVDGTTEEPCSLQASTTNDPGCRARISQLEKELIEDLKALRHKQVIHPGLQEVGLKLNSVDPTMSIDLKYLGVQLPLAPATSFPFWNLTGTNPASPDAGFPFVSRTGKTNDFTKIKGWRGKFHSASASRNEGGNSEGSLKNRSAFCSDKLDEYLENEGKLMETNMGFSSSAPTSPVVYQLPTKSTSYVRTLDSVLKKQSTISPCTSYSLKPHSVPSASRKVKSQNKQATFSGRTKSSYKSILPYPISPKQKHTHIIPGDKVTKNSSSTISENPVNNFVVPTLDENTFPKQISLRQAHQQQQQQQQGTRPPGLSKSQVKLMDLEDCALWEGKPRTYITEERADVSLTTLLTAQASLKTKPIHTIIRKRAPPCNNDFCRLGCVCSSLALEKRQPAHCRRPDCMFGCTCLKRKVVLVKGGSKTKHFQRKAAHRDPVFYDTLGEEQREGEEGVKEEEEQLKEKKKRKKLEYTICETEPEQPVRHYPLWVKVEGEVDPEPVYIPTPSVIEPMKPLLLPQPEVLSTTVKGKLLTGIKPARAYTPKPNPVIREEDKDPVYLYFESMMTCARVRVYERKKEEQKQPSPSASPSFQQQGSCHSSPENCNAKELNSEQQPLKPITCDLEDDSDKSQEKSWKSSCNEGESSSTSYVHQRSPGGPTKLIEIISDCNWEEDRNKILSILSQHINSNMPQSLKVGSFIIELASQRKSRGEKNPPVYSSRVKISMPSCQDQDDMAEKSGSETPDGPLSPGKMDDISPVQTDALDSVRERLHGGKGLPFYAGLSPAGKLVAYKRKPSSSTSGLIQVASNAKVAASRKPRTLLPSTSNSKTASSSGTTTNRPGKNLKAFVPAKRPIAARPSPGGVFTQFVMSKVGALQQKIPGVSTPQPLTGPQKFSIRPSPVMVVTPVVSSEPVQVCSPVTAAVTTTTPQVFLENVTAVTPMTAISDVGAKETTSGATTAGVVEVSETNTSTPVTPTQSAATVNLIKTTGITTPVASVAFPKSLVASPPTITLPVASTASTSIVVVTTAASSSMVTTPTSSLGSVPIILSGIDGNPPVSQRPENAPQIPVATSQVSPNTVKRPGPRLLLIPVQQGSPTLRPVPNTQLQGHRMVLQPVRSPSGMNLFRHPNGQIVQLLPLHQLRGSNTQPNLQPVVFRNPGSVMGIRLPTPSKPSETPPSSASSSAFSVMNPVIQAVGSSPAVNVITQAPSLLSSGPSFVSQSGTLTLRISPPEPQSFASKTGSETKITYSSGGQPVGTASLIPLQSGSFALLQLPGQKPVPSSILQHVASLQMKRESQNADQKDETCFLKREQEAKKALQSEGGAIDSETNIIKQNSGAPASEEILNDSLEDEGDHLDEESLIEEDPATVKPSEHSCISGSHIGEDYKDTDEEYGIRNHNSSKEKLTVLEVKTISEKASDMTVQSVSNVQFKKPGGVKMELQKGLVDPEEKSNEFPVISKEESKLELSGSTVVEQQSNLQPEAKEKGCGDSLEKDSIRERWRKHLKGPLTEKCVGTSQECKEEADEQLIKETKTCPENSDVFQQEQGISDLLGKNGTTENTRILKTECDSWSRISSPAAFSVVPKRAPKGSRGEGHFQGHLMLSGEHIQPKQEKKGGRSSADFAVLDLEEEDEEDENDKTDDSIDEIVDVVSDYQSEEVDDVEKVNNCVEYIEDDEEQVDIETVEELSEEINVAHIKTTAAHTQTFKQPCRTHISADEKAAEKSRKAPPIPLKLKPDYWSEKLQKEAEAFAYYRRTHTANERRRRGEMRDLFEKLKITLGLLHSSKVSKSLILTRAFSEIQGLTDQADKLIGQKNLLTRKRNILIRKVSSLSGKTEEVVLKKLEYIYAKQQALEAQKRKKKMGSDEFDVSPRTSKQQEGSSASSVDLGQMFLNNRKGKPLILSRKRDQTTENTSPSNTPHTSANIVMTPQGQLLTLKGPLFSGPVVTVSPALLEADLKPQVASSAMAQSENDDLFMMPRIVNVTSLATEGGLVEMGGSKYPHELPDGKPPDHLQNTVRNEDNSFEDSGRISSRGNHRDGRMALGPTQVFLANKDSGFPQIVDVSSMQEAQEFLPKKISGDMRGIQYKWKESESRRERLKPKESPFHKLKMKDLKDSSIEMELRKVASAIEEAALDPNELLTNMEDEDDTDETLTSLLNEIAFLNQQLNDDSVSLAELPSSMDAEFPGDARRAFISKLPPGNRTTFQVGHLGTDLKELPDVQGESDSVSPLLLHLEDDDFSESEKQLAEPASEPDVLKIVIDSEIKDPLLSHRKANDGGKNTSGVPAEPESVSSPPILHMKAGLENSSTDTLWRPMPKLAPLGLKVANPSSDADGQSLKVMPCLAPVAAKVGSVGHKINLTGNDQECRESKVMPTLAPIVAKLGNSGASPSSAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MK30</accession>
    <name>A0A8C4MK30_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein SDA1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SDAD1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <text evidence="1 7">Required for 60S pre-ribosomal subunits export to the cytoplasm.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
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                <person name="Guo"/>
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                <person name="Sun"/>
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                <person name="Wang"/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Zhang"/>
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                <person name="Ji"/>
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                <person name="Jin"/>
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                <person name="Yue"/>
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                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="M."/>
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                <person name="Li"/>
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                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
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        </citation>
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        <text evidence="2">Protein modification; protein glycosylation.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
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            <topology evidence="1">Single-pass type II membrane protein</topology>
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    <comment type="similarity">
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        <property type="match status" value="1"/>
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    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="464" mass="54120" checksum="CEF96F784AE2E1C0" modified="2023-09-13" version="2">MPEENVLTSSCSWEEVHGPSRMKTFRCCFKYPLQQKVFVLFLTLWLFSLLKLLNVSRLLFPQRGIYLVEYSLSTSPFVRNRYTQVKGEAGYEVNCSAVYEQEPLEIGKSLEIRRRDIIDLDDDDVGAMTSDCDIYQTLRRYHQKLVSREEKSFPIAYSLVVHKDAIMVERLIQAIYNRHNIYCIHYDRKSPEAFKVAMNNLAKCFSNIFIASKLETVQYAHISRLQADLNCLSDLLRSSVQWKYVINLCGQDFPLKSNFELVSELKKLNGANMLETVKPPHSKIERFTYRHELRQLPYEYVKLPVRTNVSKDAPPHDIEIFVGSAYFVLSRAFVKYVFNTSLVKDFFAWSEDTYSPDEHFWATLIRVPGVPGEISRSAQDVSDLQSKTRLVKWNYHEGLFYPRCTGSHLRSVCIYGAAELRWLIKDGHWFANKFDSKVDPVLIKCLAEKLEEQQREWITLSSEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MLU1</accession>
    <name>A0A8C4MLU1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">DnaJ heat shock protein family (Hsp40) member B12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">DNAJB12</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MLU1"/>
    <dbReference type="Ensembl" id="ENSEAST00005026444.2">
        <property type="protein sequence ID" value="ENSEASP00005024374.1"/>
        <property type="gene ID" value="ENSEASG00005016559.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106830620"/>
    <dbReference type="KEGG" id="eai:106830620"/>
    <dbReference type="CTD" id="54788"/>
    <dbReference type="GeneTree" id="ENSGT00940000155590"/>
    <dbReference type="OMA" id="ARSREHN"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031965">
        <property type="term" value="C:nuclear membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030544">
        <property type="term" value="F:Hsp70 protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071218">
        <property type="term" value="P:cellular response to misfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.110:FF:000004">
        <property type="entry name" value="DnaJ (Hsp40) homolog, subfamily B, member 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051100">
        <property type="entry name" value="DnaJ_subfamily_B/C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015399">
        <property type="entry name" value="DUF1977_DnaJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908">
        <property type="entry name" value="AT29763P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43908:SF8">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09320">
        <property type="entry name" value="DUF1977"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="J" evidence="8">
        <location>
            <begin position="145"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="83"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="83"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005024374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="412" mass="46098" checksum="1DEBFF73964AA9CA" modified="2022-01-19" version="1">MSSFCARLPATRRPLAQPFARPASPSLVPRSGSAMESNKDEAERCISIALKAIQSSQPERALRFLEKAQRLYPTQRVRTLIESLNQKPQSASDQPQPTDTTRATHRKAGGADAPSANGEAGGGESTKGYTAEQVAAVKRVKQCKDYYEILGVSRGASDEDLKKAYRKLALKFHPDKNHAPGATEAFKAIGTAYAVLSNPEKRKQYDQFGDDKSQAARHGHGHGDFHRGFEADISPEDLFNMFFGSGFPSSNVHVYSNGRMRYTYQQRQDRRENQGDGGLGVFVQLMPILILILVSALSQLMVSSPPYSLSPRPSVGHIHKRVTDHLKVIYYVADTFSEEYTGSSLKTVERNVEDDYIANLRNNCWKEKQQKEGLLYRARYFGDTDMYHKAQKMGTPSCNRLSETMKSLENFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MPS0</accession>
    <name>A0A8C4MPS0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Probable ATP-dependent RNA helicase DDX46</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">DEAD box protein 46</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">DDX46</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, DDX46 plays essential roles during assembly of pre-spliceosome and proofreading of the branch site.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 27">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="19">Component of the 17S U2 SnRNP complex, a ribonucleoprotein complex that contains small nuclear RNA (snRNA) U2 and a number of specific proteins. Within the 17S U2 SnRNP complex, DDX46 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Recruited to the 17S U2 SnRNP complex following release of DDX42; DDX42 and DDX46 bind the SF3B subcomplex in a competitive manner.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus speckle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Cajal body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the DEAD box helicase family. DDX46/PRP5 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="RefSeq" id="XP_014683907.1">
        <property type="nucleotide sequence ID" value="XM_014828421.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MPS0"/>
    <dbReference type="Ensembl" id="ENSEAST00005032677.2">
        <property type="protein sequence ID" value="ENSEASP00005030064.2"/>
        <property type="gene ID" value="ENSEASG00005020329.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106822993"/>
    <dbReference type="CTD" id="9879"/>
    <dbReference type="GeneTree" id="ENSGT00940000157753"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015030">
        <property type="term" value="C:Cajal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd17953">
        <property type="entry name" value="DEADc_DDX46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22473">
        <property type="entry name" value="KH-I_DDX46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000079">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000584">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056149">
        <property type="entry name" value="PRP5/DDX46/KHDC4_KH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23469">
        <property type="entry name" value="KH_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="12" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="24">
        <location>
            <begin position="372"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="22">
        <location>
            <begin position="403"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="23">
        <location>
            <begin position="592"/>
            <end position="753"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="20">
        <location>
            <begin position="372"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="26"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="21">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="112"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="21">
        <location>
            <begin position="219"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00038511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00050029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00050042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00064119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030064.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1031" mass="117362" checksum="2E6B02BF8394AEBD" modified="2023-09-13" version="2">MGRESRHYRKRSASRGRSGSRSRSRSPSDKRSKRGDDRRSRSRDRDRRRERSRSRDKRRSRSRDRKRLRRSRSRERDRSRERRRSRSRDRRRSRSRSRGRRSRSSSPGNKSKKAENRSRSKEKTDSGESSKEKKKDKDDKEDEKEKDAGNFDQNKLEEEMRKRKERVEKWREEQRKKAMENIGELKKEIEEMKQGKKWSLEDDDDDEDDPAEAEKEGNEMEGEELDPLDAYMEEVKEEVKKFNMRSVKGGGGNEKKSGPTVTKVVTVVTTKKAVVDSDKKKGELMENDQDAMEYSSEEEEVDLQTALTGYQTKQRKLLEPVDHGKIEYEPFRKNFYVEVPELAKMSQEEVNVFRLEMEGITVKGKGCPKPIKSWVQCGISMKILNSLKKHGYEKPTPIQTQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMFRHIMDQRSLEEGEGPIAVIMTPTRELALQITKECKKFSKTLGLRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLAANSGRVTNLRRVTYVVLDEADRMFDMGFEPQVMRIVDNVRPDRQTVMFSATFPRAMEALARRILSKPIEVQVGGRSVVCSDVEQQVIVIEEEKKFLKLLELLGHYQESGSVIIFVDKQEHADGLLKDLMRASYPCMSLHGGIDQYDRDSIINDFKNGTCKLLVATSVAARGLDVKHLILVVNYSCPNHYEDYVHRAGRTGRAGNKGYAYTFITEDQARYAGDIIKALELSGTAVPPDLEKLWSDFKDQQKAEGKIIKKSSGFSGKGFKFDETEQALANERKKLQKAALGLQDSDDEDAAVDIDEQIESMFNSKKRVKDMAAPGTSSVPAPTAGNAEKLEIAKRLALRINAQKNLGIESQDVMQQATNAILRGGTILAPTVSAKTIAEQLAEKINAKLNYVPLEKQEEERQDGGQNESFKRYEEELEINDFPQTARWKVTSKEALQRISEYSEAAITIRGTYFPPGKEPKEGERKIYLAIESANELAVQKAKAEITRLIKEELIRLQNSYQPTNKGRYKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MQB6</accession>
    <name>A0A8C4MQB6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">E3 ubiquitin-protein ligase HERC2</fullName>
            <ecNumber evidence="5">2.3.2.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="22">HECT domain and RCC1-like domain-containing protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="23">HECT-type E3 ubiquitin transferase HERC2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">HERC2</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">E3 ubiquitin-protein ligase that regulates ubiquitin-dependent retention of repair proteins on damaged chromosomes. Recruited to sites of DNA damage in response to ionizing radiation (IR) and facilitates the assembly of UBE2N and RNF8 promoting DNA damage-induced formation of 'Lys-63'-linked ubiquitin chains. Acts as a mediator of binding specificity between UBE2N and RNF8. Involved in the maintenance of RNF168 levels. E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of XPA which influences the circadian oscillation of DNA excision repair activity. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway. Also modulates iron metabolism by regulating the basal turnover of FBXL5.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Microtubule organizing center</location>
            <location evidence="2">Centrosome</location>
            <location evidence="2">Centriole</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="5"/>
    <dbReference type="Ensembl" id="ENSEAST00005033328.2">
        <property type="protein sequence ID" value="ENSEASP00005030654.2"/>
        <property type="gene ID" value="ENSEASG00005020256.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154975"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030182">
        <property type="term" value="P:neuron differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd08664">
        <property type="entry name" value="APC10-HERC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14402">
        <property type="entry name" value="UBA_HERC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02344">
        <property type="entry name" value="ZZ_HERC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000015">
        <property type="entry name" value="E3 ubiquitin-protein ligase HERC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.30:FF:000003">
        <property type="entry name" value="E3 ubiquitin-protein ligase HERC2 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
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                <person name="Zhao"/>
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                <person name="Wang"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="J."/>
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                <person name="A."/>
                <person name="Amills"/>
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                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <location evidence="10">Nucleus</location>
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    </dbReference>
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        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24083">
        <property type="entry name" value="NUCLEAR HORMONE RECEPTOR"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00104">
        <property type="entry name" value="Hormone_recep"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="zf-C4"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="RETINOIDXR"/>
    </dbReference>
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        <property type="entry name" value="STRDHORMONER"/>
    </dbReference>
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        <property type="entry name" value="STROIDFINGER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00430">
        <property type="entry name" value="HOLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00399">
        <property type="entry name" value="ZnF_C4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Nuclear receptor ligand-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 10" id="KW-0863">Zinc-finger</keyword>
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            <end position="276"/>
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            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
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            <end position="182"/>
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    <feature type="region of interest" description="Disordered" evidence="11">
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    <feature type="compositionally biased region" description="Low complexity" evidence="11">
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    <feature type="compositionally biased region" description="Pro residues" evidence="11">
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    <feature type="compositionally biased region" description="Pro residues" evidence="11">
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    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="319"/>
            <end position="328"/>
        </location>
    </feature>
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        <source>
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        </source>
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    </evidence>
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        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MTI7</accession>
    <name>A0A8C4MTI7_EQUAS</name>
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        <recommendedName>
            <fullName evidence="3">Serine/threonine-protein phosphatase 1 regulatory subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">PPP1R10</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Huang"/>
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                <person name="Wang"/>
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                <person name="Fang"/>
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                <person name="Zhao"/>
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                <person name="Wang"/>
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                <person name="Zhang"/>
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                <person name="Wang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
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                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
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                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="G."/>
                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="15">Component of the PNUTS-PP1 complex (also named PTW/PP1 complex), composed of PPP1R10/PNUTS, TOX4, WDR82, and PPP1CA (or PPP1CB or PPP1CC).</text>
    </comment>
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        <subcellularLocation>
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        <subcellularLocation>
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        </subcellularLocation>
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        <property type="protein sequence ID" value="ENSEASP00005027819.2"/>
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        <property type="component" value="Chromosome 8"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Serine/threonine-protein phosphatase 1 regulatory subunit 10"/>
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    </dbReference>
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        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
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        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
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        <property type="entry name" value="Znf_CCCH"/>
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    <dbReference type="InterPro" id="IPR036855">
        <property type="entry name" value="Znf_CCCH_sf"/>
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    <dbReference type="PANTHER" id="PTHR46557">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 1 REGULATORY SUBUNIT 10-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="Med26"/>
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    </dbReference>
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        <property type="entry name" value="ZnF_C3H1"/>
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    <keyword evidence="6" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8 17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="14 16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
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            <begin position="73"/>
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        </location>
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            <begin position="153"/>
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            <begin position="247"/>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8C4MYM8_EQUAS</name>
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        <recommendedName>
            <fullName evidence="3">mitogen-activated protein kinase kinase kinase</fullName>
            <ecNumber evidence="3">2.7.11.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="primary">MAP3K2</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.25" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4MYM8"/>
    <dbReference type="Ensembl" id="ENSEAST00005035807.2">
        <property type="protein sequence ID" value="ENSEASP00005032857.2"/>
        <property type="gene ID" value="ENSEASG00005022464.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000156884"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071260">
        <property type="term" value="P:cellular response to mechanical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06405">
        <property type="entry name" value="PB1_Mekk2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06652">
        <property type="entry name" value="STKc_MEKK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000071">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000026">
        <property type="entry name" value="Mitogen-activated protein kinase kinase kinase 3 isoform 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034879">
        <property type="entry name" value="PB1_MEKK2/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584:SF369">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PB1" evidence="17">
        <location>
            <begin position="43"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="Protein kinase" evidence="16">
        <location>
            <begin position="356"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="25"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="201"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="290"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="203"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="300"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="385"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022741"/>
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            <dbReference type="ARBA" id="ARBA00022777"/>
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            <dbReference type="ARBA" id="ARBA00047559"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
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            <dbReference type="Ensembl" id="ENSEASP00005032857.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="619" mass="69791" checksum="F88765084C4CBE37" modified="2023-09-13" version="2">MDDQQALNSIMQDLAVLHKASRPALSLQETRKTKSSSPKKQNDVRVKFEHRGEKRILQFPRPVKLEDLRSKAKIAFGQTMDLHYTNNELVIPLTTQDDLDKAVELLDRSIHMKSLKILLVINGSTQATNLEPLPSLEDLDNTVFGAERKKRLSIIGPTSRDRSSPPPGYIPDELHQVARNGSFTSINSEGEFIPESMDQMLDPLSLSSPENSGSGSCPSLDSPLDGESYPKSRMPRAQSYPDNHQEFSDYDNPIFEKFGKGGTYPRRYHVSYHHQDYNDGRKTFPRARRTQGTSFRSPVSFSPTDHSLSTSSGSSIFTPEYDDTRIRRRGSDIDNPTLTVMDISPPSRSPRAPTNWRLGKLLGQGAFGRVYLCYDVDTGRELAVKQVQFDPDSPETSKEVNALECEIQLLKNLLHERIVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENVTRKYTRQILEGVHYLHSNMIVHRDIKGANILRDSTGNVKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPEVISGEGYGRKADVWSVGCTVVEMLTEKPPWAEFEAMAAIFKIATQPTNPKLPPHVSDYTRDFLKRIFVEAKLRPSADELLRHMFVHYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MYP9</accession>
    <name>A0A8C4MYP9_EQUAS</name>
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        <recommendedName>
            <fullName evidence="5">Nebulin</fullName>
        </recommendedName>
    </protein>
    <organism evidence="3 4">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="Ensembl" id="ENSEAST00005037410.2">
        <property type="protein sequence ID" value="ENSEASP00005034322.2"/>
        <property type="gene ID" value="ENSEASG00005023364.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154533"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030018">
        <property type="term" value="C:Z disc"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071691">
        <property type="term" value="P:cardiac muscle thin filament assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055297">
        <property type="entry name" value="NEBU/NEBL"/>
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    <dbReference type="InterPro" id="IPR013998">
        <property type="entry name" value="Nebulin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000900">
        <property type="entry name" value="Nebulin_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11039">
        <property type="entry name" value="NEBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11039:SF37">
        <property type="entry name" value="NEBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00880">
        <property type="entry name" value="Nebulin"/>
        <property type="match status" value="42"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00510">
        <property type="entry name" value="NEBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00227">
        <property type="entry name" value="NEBU"/>
        <property type="match status" value="75"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51216">
        <property type="entry name" value="NEBULIN"/>
        <property type="match status" value="54"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005034322.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2617" mass="305243" checksum="5785E6D1047C2615" modified="2023-09-13" version="2">MTGYDLRADAIGFQHAKASRDIASDYLYKTTYEKQKGHYIGLPSAKEDPKLVWAAKVMKMQNDRLYKKVYNDHKAKITIPVDMVSITAAKEGQELASNVDYRQYLHHWSCFPDQNDVIQARKAYDLQSDAIYKADLEWLRGIGWMPEGSPEILRVRNAQKIFLDSVYRTPVVKLKYSSIVDTPEVVLAKANAENISIPKYREVWDRDKTSIHIMPDTPEINLAKVNAVNVSKNIYRKGWDEMKMSCDVRLDAIPIQAAKASREIASDYKYKLDHEKQKGHYVGTRTAKDDNKIRWALIAGKIQNEREYRLPWAKWKTKFQSPADMLSITHSKHSQTLVSDIDYHNYLHQWTCLPDQNDVIQAKKAYELQSDAVYKADLEWLRGIGWLPSDSVSVNHAKHAADIFSEKKYRTKIETLNFTPVDDRVDYVTAKQSGEILNDIKYRKDWNDTKSKYTLTETPLLHTAQEAARILDQYLYKEGWEKQKATGYILPPDAVPFVHAHHSGDVQSELKYKAEHVKQRGHYVGVPTMRDDPKLVWFEHAGQIQNDRLYKEQYHKTKAKINIPPDMVSVLAAKEGQTLASDIDYRHYLHQWMCHPDQNDVIQARKAYDLQSDNIYKADLEWLRGIGWIPLDSVDHVRVTRNQEMVNQIKYKKDALDNYPNFTSVVDPPAVVLAKINSVNQSDVKYKETFNKEVKGKYIFSPDTPYITHSKDMGKLYSTILYKGAWEGTKAYGYTLDERYIPIVGAKHADYVNSELKYKETYEKLKGHYLAGKEISEFPAVVHCLDFQKMRSVLNYRRQYEDTKANVHIPIDMMNHVLAKRCQYILSDLEYRHYFHQWTSLPGEPSVILARNAQEILSDNVYKDDLNWLRGIGCYVWDTPQILHAKKAYDLQSQIQYTAAGKENLQNYNLVTDTPLYVTALQSGINASEVKYKENYHQIKDKYTTILETVDYDRIKNLKDLYSSNLYKEAWDKVKATSYILPSSTLSLTHAKNQKHLASIVKYREEYEKFKALYTLPKSVDDDPNTARCLRVGKFNIDRLYRSVYEKNKMKIHIVPDMVEMVTAKDSQKKVSEIDYRLHLHEWICHPDLKVNSHVRKVTDQISDIVYKDDLNWLKGIGCYVWDTPEILHAKHAYDLRNDIKYKEHVRKTRNDYKLVTDTPVYVQAVKSGKQLSDAVYHHDYVHSVRGKVAPTTKTVDLDRALHAYKLQSENLYRRPGLRSLPTGYRLPVDTPHFKHTKDTRYMSSYFKYKEVYEHIKAHGYTLGPNDVPFVNVRRVNNVTSERLYRQLYHKLKDKIHTTPDTPEIRQVKKTQEAVSELIYKSDFFKMQGHMISMPYTPQVIHCRYVGDITSDIKYKEDLRILRGLGCFLYDTPDMVRSRHLRKLWSHYLYTDNARKIRDKYKVVLDTPEYRKVQELKTHLSELVYRAAGRKQKSVFTSVPDTPDLLRAKRGQKLQSQYLYVELATKERPHHHAGNQTTALKHARDVKDMVSETKYKIQYEKMKDRYTPVPDTPILIRAKRAYWNASDLRYKETFQKTKGKYHTVKDALDIVYHRKVTDDISKVKYKKNYMSQLGIWRSIADRPEHFHHRAVTDAISDIKYKSDLTWLKGIGCYAYDTPDFTLAEKNKTLYSKYKYKEVFERIKSDFKYVADCPINRHFKYATQLMNEKKYRADYEQRKDKYHLVVDEPRHLLAKITGDQISQIRYRKNYEKTKDKFTSIVDTPEHLRTTKVNKQISDILYKLEYNKARPRGYTTIHDTPMLLHVRKVKDEVSDIKYKEVYQRNKSNCTIKPDASYIKAAKDAYKVNTNLDYKKQYEANKAHWRWIPDRPDFIQAAKSSLQQSDFEYKLDREFLRGCKLSVTDDKDMVLALRNSLIESDLKYKEKHVKERGSCHAVPDTPQILLAKTVSNLVSENKYKAYVKKRLAQGSYTTLPETRDTVHVKEVTKNVSDTNYKKKFVKERGKSNYSIMLEPPDVKHAMEVAKKQSDVAYRKSAKENLHYTTVADRPDIKKATQAAKQASEVEYRAKHRKEGSHGLSMLGRPDIEMAKKAAKLSSQVQYRENFNKEKGKTPKYNPKESQLYKVMKDANNLASEVKYKADLKKLHKPVTDMKESLIMHHVLNTSQLASSYQYKKNYEKSKGHYHTIPDNLEQLHLKEATELQSIVKYKEKYEKERGKPMLDFETPTYITAKESQQMQSGKEYRKEYDESIKGRNLTGLEVTPALLHVKYATKIASEKEYRRDLEESIRGKGLSEMEDTPDMLRAKNATQILNEKEYKRDLELEVKGRGLNAMANETPDFMRARNATDIASQIKYKQLAEMEKANFTSVVDTPEIIHAQQVKNLSSKKKYKEDAEKCMSYYETVLDTPEMQRVRENQKNFSLLQYQCDLKNSKGKITVVQDTPEILRVKENQKNFSSVLYKEDVSPGTAIGKTPEMMRVKQTQDHISSVKYKEVIGQGTPIPDLPEVKRVKETQKHISSVMYKENLGTGIPTPVTPEIERVKRNQENFSSVLYKENLGKGTPTPVTPEMERVKRNQENFSSILYKENLSKGTPLPVTPEMERVKRNQENFSSILYKENLSKGTPLPVTPEMERVKRNQENFSSVYLRGQKIKSLNSYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MZ85</accession>
    <name>A0A8C4MZ85_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Homeobox protein DLX-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">DLX1</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a role as a transcriptional activator or repressor. Inhibits several cytokine signaling pathways, such as TGFB1, activin-A/INHBA and BMP4 by interfering with the transcriptional stimulatory activity of transcription factors, such as MSX2, FAST2, SMAD2 and SMAD3 during hematopoietic cell differentiation. Plays a role in terminal differentiation of interneurons, such as amacrine and bipolar cells in the developing retina. Likely to play a regulatory role in the development of the ventral forebrain. May play a role in craniofacial patterning and morphogenesis and may be involved in the early development of diencephalic subdivisions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Interacts with SMAD4 (via homeobox DNA-binding domain). Interacts (via homeobox DNA-binding domain) with POU4F2; this interaction suppresses DLX1-mediated transcriptional activity in postnatal retina and enhances retinal ganglion cell (RGC) differentiation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the distal-less homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014702064.1">
        <property type="nucleotide sequence ID" value="XM_014846578.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070364205.1">
        <property type="nucleotide sequence ID" value="XM_070508104.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MZ85"/>
    <dbReference type="SMR" id="A0A8C4MZ85"/>
    <dbReference type="Ensembl" id="ENSEAST00005036075.2">
        <property type="protein sequence ID" value="ENSEASP00005033100.1"/>
        <property type="gene ID" value="ENSEASG00005022643.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106834718"/>
    <dbReference type="KEGG" id="eai:106834718"/>
    <dbReference type="CTD" id="1745"/>
    <dbReference type="GeneTree" id="ENSGT00940000160029"/>
    <dbReference type="OMA" id="GPPVWNT"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 4"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071773">
        <property type="term" value="P:cellular response to BMP stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071560">
        <property type="term" value="P:cellular response to transforming growth factor beta stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021893">
        <property type="term" value="P:cerebral cortex GABAergic interneuron fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048706">
        <property type="term" value="P:embryonic skeletal system development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021877">
        <property type="term" value="P:forebrain neuron fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021766">
        <property type="term" value="P:hippocampus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030514">
        <property type="term" value="P:negative regulation of BMP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903845">
        <property type="term" value="P:negative regulation of cellular response to transforming growth factor beta stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043524">
        <property type="term" value="P:negative regulation of neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048715">
        <property type="term" value="P:negative regulation of oligodendrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046533">
        <property type="term" value="P:negative regulation of photoreceptor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014016">
        <property type="term" value="P:neuroblast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051402">
        <property type="term" value="P:neuron apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007219">
        <property type="term" value="P:Notch signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042475">
        <property type="term" value="P:odontogenesis of dentin-containing tooth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048709">
        <property type="term" value="P:oligodendrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902871">
        <property type="term" value="P:positive regulation of amacrine cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009954">
        <property type="term" value="P:proximal/distal pattern formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021544">
        <property type="term" value="P:subpallium development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000074">
        <property type="entry name" value="Distal-less homeobox 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050460">
        <property type="entry name" value="Distal-less_Homeobox_TF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020479">
        <property type="entry name" value="HD_metazoa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000047">
        <property type="entry name" value="HTH_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327">
        <property type="entry name" value="HOMEOBOX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24327:SF33">
        <property type="entry name" value="HOMEOBOX PROTEIN DLX-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00024">
        <property type="entry name" value="HOMEOBOX"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00031">
        <property type="entry name" value="HTHREPRESSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4 10 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 10 11" id="KW-0371">Homeobox</keyword>
    <keyword evidence="6 10 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="13">
        <location>
            <begin position="126"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="10">
        <location>
            <begin position="128"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="95"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="204"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="25"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00053292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067381"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <person name="X. and Zhong"/>
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            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RNase PH family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PIU5"/>
    <dbReference type="Ensembl" id="ENSEAST00005009605.2">
        <property type="protein sequence ID" value="ENSEASP00005008817.2"/>
        <property type="gene ID" value="ENSEASG00005006327.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153348"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000177">
        <property type="term" value="C:cytoplasmic exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000791">
        <property type="term" value="C:euchromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000176">
        <property type="term" value="C:nuclear exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045006">
        <property type="term" value="P:DNA deamination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071028">
        <property type="term" value="P:nuclear mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071051">
        <property type="term" value="P:poly(A)-dependent snoRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016075">
        <property type="term" value="P:rRNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034475">
        <property type="term" value="P:U4 snRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11372">
        <property type="entry name" value="RNase_PH_RRP46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.70">
        <property type="entry name" value="GHMP Kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001247">
        <property type="entry name" value="ExoRNase_PH_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015847">
        <property type="entry name" value="ExoRNase_PH_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036345">
        <property type="entry name" value="ExoRNase_PH_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027408">
        <property type="entry name" value="PNPase/RNase_PH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050080">
        <property type="entry name" value="RNase_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11953:SF1">
        <property type="entry name" value="EXOSOME COMPLEX COMPONENT RRP46"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01138">
        <property type="entry name" value="RNase_PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03725">
        <property type="entry name" value="RNase_PH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55666">
        <property type="entry name" value="Ribonuclease PH domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0271">Exosome</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="7">
        <location>
            <begin position="59"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="domain" description="Exoribonuclease phosphorolytic" evidence="8">
        <location>
            <begin position="113"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008817.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="197" mass="21257" checksum="1ABB56AE13BFF2C4" modified="2023-09-13" version="2">MEGVMRSDAKICGESGTESSPRGLGCSLRHFACEQNLLSRPDGSASFLQGVAEKSREWLIRNTCEAVVLGTLHPRTSITVVLQVISDAGSLLACCLNAACMALVDAGVPMRALFCGVTCALDSDGTLMLDPTAKQEKEARAVLTFALDSVERKLLMSTTKGLYSDAELQQCLAAAQAASQHVFRFYRESLQRRYSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PJ49</accession>
    <name>A0A8C4PJ49_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA replication licensing factor MCM2</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">DNA replication licensing factor mcm2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="22">Minichromosome maintenance protein 2 homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Nuclear protein BM28</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="primary">MCM2</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="25 26" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="25" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="25" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="19">Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="RefSeq" id="XP_014701587.1">
        <property type="nucleotide sequence ID" value="XM_014846101.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PJ49"/>
    <dbReference type="Ensembl" id="ENSEAST00005010296.2">
        <property type="protein sequence ID" value="ENSEASP00005009477.1"/>
        <property type="gene ID" value="ENSEASG00005006759.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106834468"/>
    <dbReference type="KEGG" id="eai:106834468"/>
    <dbReference type="CTD" id="4171"/>
    <dbReference type="GeneTree" id="ENSGT01150000286951"/>
    <dbReference type="OMA" id="TYERVTT"/>
    <dbReference type="OrthoDB" id="844at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000228">
        <property type="term" value="C:nuclear chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd17753">
        <property type="entry name" value="MCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000002">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000005">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000138">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008045">
        <property type="entry name" value="MCM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059098">
        <property type="entry name" value="WHD_MCM2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF44">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12619">
        <property type="entry name" value="MCM2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23669">
        <property type="entry name" value="WHD_MCM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01658">
        <property type="entry name" value="MCMPROTEIN2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12" id="KW-0347">Helicase</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="24">
        <location>
            <begin position="474"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="23">
        <location>
            <begin position="116"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="23">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="62"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="23">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="23">
        <location>
            <begin position="116"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00054135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00074927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00078742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00079628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="904" mass="101872" checksum="CF18CDAA57EB3837" modified="2022-01-19" version="1">MAESSESFTTASSPARRRRSNDPLTSSPGRSSRRTDALTSSPGRDLPPFEDESEGLLGTEGPLEEEEEDGEELIGDGMERDYRAIPELDTYEAEGMALDDEDVEELTASQREAAERVMRQRDREAGRGLGRMRRGLLYDSDEEDEERPARKRRQVERATEDGEEDEEMIESIENLEDLKGHSVREWVSMAGPRLEIHHRFKNFLRTHVDGHGHNVFKERISDMCKENRESLVVNYEDLAAREHVLAYFLPEAPAELLQIFDEAALEVVLAMYPKYDRIASHIHVRISHLPLVEELRSLRQLHLNQLIRTSGVVTSCTGVLPQLSMVKYNCNKCSFVLGPFCQSQNQEVKPGSCPECQSAGPFEVNMEETIYQNYQRIRIQESPGKVAAGRLPRSKDAILLADLVDSCKPGDEIELTGIYHNNYDGSLNTANGFPVFATVILANHVAKKDNKVAVGELTDEDVKMIISLSKDQQIGEKIFASIAPSIYGHEDIKRGLALALFGGEPKNPGGKHKVRGDINVLLCGDPGTAKSQFLKYIEKVSSRAIFTTGQGASAVGLTAYVQRHPVSREWTLEAGALVLADRGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQARCTVIAAANPIGGRYDPSLTFSENVDLTEPIISRFDVLCVVRDTVDPVQDEMLARFVVGSHVRHHPSNKEEEGLGGGTPEPAMPNTYGVEPLPQEVLKKYIIYAKEKVHPKLNQMDQDKVAKMYSDLRKESMATGSIPITVRHIESMIRMAEAHARIHLRDYVIEDDVNMAIRVMLESFIDTQKFSVMRSMRKTFSRYLSFRRDNNELLLFILKQLVAEQVTYQRNRFGAQQDTIEVPEKDLVDKARQINIHSLSAFYDSELFRMNKFSHDLKRKMILQQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PKW5</accession>
    <name>A0A8C4PKW5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="18">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="primary">MAN1A1</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + 4 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 4 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56008"/>
            <dbReference type="Rhea" id="RHEA-COMP:14356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:139493"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + 3 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 3 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56028"/>
            <dbReference type="Rhea" id="RHEA-COMP:14358"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60628"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="14">Endomembrane system</location>
            <topology evidence="14">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="18"/>
    <dbReference type="RefSeq" id="XP_070352197.1">
        <property type="nucleotide sequence ID" value="XM_070496096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PKW5"/>
    <dbReference type="Ensembl" id="ENSEAST00005014441.2">
        <property type="protein sequence ID" value="ENSEASP00005013296.2"/>
        <property type="gene ID" value="ENSEASG00005009271.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106830488"/>
    <dbReference type="CTD" id="4121"/>
    <dbReference type="GeneTree" id="ENSGT00940000158188"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
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        <property type="entry name" value="Seven-hairpin glycosidases"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9 17" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10 18" id="KW-0326">Glycosidase</keyword>
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    <keyword evidence="7" id="KW-0735">Signal-anchor</keyword>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8C4PM91_EQUAS</name>
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        <recommendedName>
            <fullName evidence="9">Cationic amino acid transporter C-terminal domain-containing protein</fullName>
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        <name type="scientific">Equus asinus</name>
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        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <property type="gene ID" value="ENSEASG00005010846.2"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="probable cationic amino acid transporter"/>
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        <property type="entry name" value="Amino acid/polyamine transporter I"/>
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        <property type="entry name" value="AA/rel_permease1"/>
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    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF4">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="229"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="316"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="366"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="391"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="461"/>
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="8">
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    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="561"/>
            <end position="578"/>
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    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="9">
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            <begin position="531"/>
            <end position="580"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="7">
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            <begin position="418"/>
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            <dbReference type="ARBA" id="ARBA00004141"/>
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            <dbReference type="Pfam" id="PF13906"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
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            <dbReference type="Proteomes" id="UP000694387"/>
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            <authorList>
                <consortium name="Ensembl"/>
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</entry>
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                <person name="Liu"/>
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                <person name="Pan"/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0I6W7"/>
    <dbReference type="Ensembl" id="ENSEAST00005049077.1">
        <property type="protein sequence ID" value="ENSEASP00005035491.1"/>
        <property type="gene ID" value="ENSEASG00005036227.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282514"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0807">Transducer</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="135"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="179"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005035491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="23554" checksum="AF40940C5ABFEA70" modified="2023-09-13" version="1">MYFFLSHLSSVTSAIPQQLGPRCWWTSLPRASQSPSLAWLCDFLSSLSFQILSVYCWQWWPLIGTRTLEAHCSTVNMSSRACSLLVAGVYLVGIADALIHTTLTFCLGFSGSNEMSHFFCHISPPLSLSYSDTQINELVICTIIGFTELSTISGVLVSYCCIILSFLKIHTAEGRLKAFSTCVSHLTAVAISQGTTLFMYFRASPSYTLDQDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I794</accession>
    <name>A0A9L0I794_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peptidase S1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">LOC123276441</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S1 family. CLIP subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070338384.1">
        <property type="nucleotide sequence ID" value="XM_070482283.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0I794"/>
    <dbReference type="Ensembl" id="ENSEAST00005064003.1">
        <property type="protein sequence ID" value="ENSEASP00005035656.1"/>
        <property type="gene ID" value="ENSEASG00005026599.1"/>
    </dbReference>
    <dbReference type="GeneID" id="123276441"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009003">
        <property type="entry name" value="Peptidase_S1_PA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043504">
        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051487">
        <property type="entry name" value="Ser/Thr_Proteases_Immune/Dev"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24256">
        <property type="entry name" value="TRYPTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50240">
        <property type="entry name" value="TRYPSIN_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040287728" description="Peptidase S1 domain-containing protein" evidence="3">
        <location>
            <begin position="18"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="4">
        <location>
            <begin position="9"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005035656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="274" mass="30076" checksum="30F8B052C2F4FBFD" modified="2023-09-13" version="1">MLPLLLPLLFGPMPAEAYSRKGPATWPWQASIFLDTRYRCEGALISPEWVLTGASCFGSWPSSLFSVTLGPNRLALDACESTSSVEELLLSPGGPEGSGPGGLALARLERPPSLSSAVQPVSLATGPQPSPWPKLCWAQGFDLDFDPYIPPKELHSVPLKPLDVSSCKDAFRLQPDCPDLKVSLPKGSQCTRLPYSYSKLVVSDGAPLVCSQGRNWLLQGVMTWSPCLGTRLPEVYMPVYPFRSWIREKVSNATFYTLAKKCPLILPKTRKRRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IAJ7</accession>
    <name>A0A9L0IAJ7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Complex I-B14</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">NADH-ubiquinone oxidoreductase B14 subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">NDUFA6</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed to be not involved in catalysis. Required for proper complex I assembly. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Mammalian complex I is composed of 45 different subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I LYR family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IAJ7"/>
    <dbReference type="Ensembl" id="ENSEAST00005052532.1">
        <property type="protein sequence ID" value="ENSEASP00005037822.1"/>
        <property type="gene ID" value="ENSEASG00005024363.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000018898"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 6"/>
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        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE B14 SUBUNIT"/>
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    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
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            <begin position="1"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011790"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00016386"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00030213"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00032352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005037822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="267" mass="29857" checksum="14110AE0EE80603B" modified="2023-09-13" version="1">MRLSLSRRLGAAPASDRGSSPTPCAPTGVPNERRSTARAGFPPSLQPVARAPPGGAVGPRLGRPTAERAFPQMSMRRPHLPIFSRPQSLSSQSKSPRAAHPWGLGAGRLILANDRRYSFFRHTRALKRGVGLGGDFLSKMAASGVSRAAAAAGTSVKPIFSRDMNEAKRRVRELYRAWYREVPNTVHLFQLDISVKQGRDKVREMFMKNAHVMDPRVVDLLVIKGKMELEETIKVWKQRTHIMRFFHETEAPRPKDFLSKFYVGHDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IBJ5</accession>
    <name>A0A9L0IBJ5_EQUAS</name>
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        <submittedName>
            <fullName evidence="7">Ankyrin repeat domain 24</fullName>
        </submittedName>
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        <name evidence="7" type="primary">ANKRD24</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Gao"/>
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                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
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                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IBJ5"/>
    <dbReference type="Ensembl" id="ENSEAST00005055594.1">
        <property type="protein sequence ID" value="ENSEASP00005038242.1"/>
        <property type="gene ID" value="ENSEASG00005038678.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159237"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042420">
        <property type="entry name" value="RAI14/UACA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24129">
        <property type="entry name" value="ANKYCORBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24129:SF0">
        <property type="entry name" value="ANKYCORBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 4" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3 5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="57"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="90"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="4">
        <location>
            <begin position="156"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="245"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="472"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="635"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="497"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="529"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1066" mass="115186" checksum="223A3E92B1297276" modified="2023-09-13" version="1">PCGGGGPAPCSSGCPPQSQDWGKSDERLLQAVENNDATRVAALITRKGLVPTKLDPEGKSAFHLAAMRGAASCLEVMLAHGANVMSTDGAGYSALHLAAKYGHPQCLKQLLQASCVVDTVDSSGWTALHHAAAGGCIACSEMLCSFKAHLNPRDRLGTTPLIIAAQMCHTDLCRLLLQQGAAANDQDLLLTSLGGGESSCPETVEVLLQGGAQPGITDALGQDAAHYGALAGDKFILHLLQEAARRPSPPSGMPNSVSSREKRGATKKRKAPQPPASIPMLDDRDAYEEIVRLRQERGRLLQKIRGLEQHQERRKQEAEASSLHSLERQVQELQQLLAEKQEEKESLGREVESLQSRLSLLENERENTSYDVATLQDEEGELPDFLGAEALLSKRLSPSAQELLASLQEQVAALRRENQELMEKVQILENFEKDEMDANGSAEVIPLALYDSLRAELDQLRRQHAEALRALEEQGAREAPGEEDAAPGEGEGLGAKTTRNGPTETQLNGTVAPETKVNGPESTEEEAAGVETTEATPTGAKATETKGVGAESLEIKAMEAEVTETNALEGGGNPETKATGVEAATTKAEEPKMKPGEVGAVEAELMGTETTDMEATGVEATDPEAAGSVLHPGAAEASEKLQAELETRIRALEEALRQREREAATELEAARGKCEAAEAEAGRLREQVREAEGGAVGVASSSDMVQLRAALEQAREDLRARDSRLRELEATAAWLDEARAGRLLAEEEARGLRAELAQREEVRLEQSRELEALRKQLATAMAAGEQQRAAAAELGRARDEAEARAAELSAACEEARRGLAELREASEALRQSAVPASEHHRLQEEALELRGRAASLEQEVVATGKEAARLRAELERERVGSVARLDHDRVVGALQAEVARLEGQLEELGRRHEKTSAEVFQVQREALFMKSERHAAEAQLAVAEQQLRGLRTEVEQARQAQSRAQEALDRAKEKDKKITELSKEVFSLKEALKEQPAAPGTPEVEALRAQVKGLQQQLEEAARGHSAVVALYRSHLLYAIQGQMDADVQRILSQILQMQRLQAQGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IEN0</accession>
    <name>A0A9L0IEN0_EQUAS</name>
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        <recommendedName>
            <fullName evidence="16">Lipid transferase CIDEC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Cell death-inducing DFFA-like effector protein C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">CIDEC</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
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        <lineage>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
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                <person name="Wang"/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
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                <person name="Ju"/>
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                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Yang"/>
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                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="Guo"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
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                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a triacyl-sn-glycerol(in) = a triacyl-sn-glycerol(out)</text>
            <dbReference type="Rhea" id="RHEA:39011"/>
            <dbReference type="ChEBI" id="CHEBI:64615"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Lipid droplet</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the CIDE family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014703614.1">
        <property type="nucleotide sequence ID" value="XM_014848128.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044611144.1">
        <property type="nucleotide sequence ID" value="XM_044755209.2"/>
    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR001478">
        <property type="entry name" value="PDZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23348">
        <property type="entry name" value="PERIAXIN/AHNAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23348:SF37">
        <property type="entry name" value="PROTEIN AHNAK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00228">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50106">
        <property type="entry name" value="PDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDZ" evidence="4">
        <location>
            <begin position="101"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="62"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="189"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="370"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="524"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="563"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="667"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="993"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1331"/>
            <end position="1356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1433"/>
            <end position="1459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1771"/>
            <end position="1796"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2107"/>
            <end position="2132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2611"/>
            <end position="2636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2777"/>
            <end position="2816"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2881"/>
            <end position="2911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="2983"/>
            <end position="3262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3280"/>
            <end position="3320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3501"/>
            <end position="3530"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3572"/>
            <end position="3599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="3613"/>
            <end position="3748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="191"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="256"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="415"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="446"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="476"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="2805"/>
            <end position="2816"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="3038"/>
            <end position="3050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="3076"/>
            <end position="3092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="3119"/>
            <end position="3140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="3247"/>
            <end position="3259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="3303"/>
            <end position="3314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="3501"/>
            <end position="3510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="3572"/>
            <end position="3586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="3587"/>
            <end position="3597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="3692"/>
            <end position="3705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="3707"/>
            <end position="3724"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005040750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="3748" mass="387960" checksum="899256738C2BE582" modified="2023-09-13" version="1">MCDCFHVVLPTWPGAPGSGAPPPLAEGNLSLSCQVTEGPVDENIRPRPQGSSPVYECATEGAGFGLPEDTPSRQVSSGRRRSWWKRDSGDSRTFSRMSCCLLRTRVMEVGASGYSVTGGGDQGIFVKQVLKDSSAAKLFNLREGDQLLSTTIFFDDIKYEDALKILQYSEPYKVQFKIRRKLPAREDEAWASSSAQRGPQGSEKQAKDVGDGSTETPTKTLEGDGDQERLISKPREGRGRRPQKERLSWPKFQTIKSKRTPGPRRSHSSSEAYERGDVPDVSPTSTDTEAQLPAEEQEQKAGPGGQRRRRFLNLKFRMGSGRGLAPVGRPGRGVQGGAVQAGVLEEPTQPGHPRVGAVGEGAALVPGRATMTKEAKVQEEPVPQEGADQGPAPGVSGEGQREGVQGLEIGIARLSLKDTADEDSTRKREPEFQIRIPTLKTPKFGFSKEKGLGTEESKTAPQRGLMEEGAGQTDTEGAREGPESVPEQEKRRAEEDVSGHKGDAKMVEKAGEGKMKMLKFKRPSFGWSPVKHPRTVGGKPTKEREKEIGGSITFVKLDIEIKDTDAIGEDTETHPPEITGEREEKMRMEGEDTLREKDVVVRDSKFRMPKFKMPSFGVSAPSKSVEASLDASLPKVQADVSLPSIQADVKTADLSVELPSAELEVKTAGAGVKLPEGPGPEGEPQEPAAGAGLKGHLPKVQMPSIKMPKVDFKAPQVDIKGPKLDVKGAKGEVTAPGVEVSLPSVEVDAQVPGAKLEGDASLGDKEVAARDSKLKMPKFKMPSFGVSAAGKDLGTSVDVPAPKVGGEATLPSVGAELTAPEGSVPVPSAEVRLPGGELEVALPEGEAAGAELKGKAEGARIKAHLPKVQMPSIKMPKVDIRAPQVDIKGPSADLKGLKGEATAPGVEVSLPSVEVDAQVPGAKLEGDASLGDKEVAARDSKFKMPNKSVEASLDASLPKVQADVSLPSIQADVKTADLSVELPSAELEVKTAGAGVKLPEGPGPEGEPQEPAAGAGLKGHLPKVQMPSIKVPKVDFKAPQVDIKGPKLDVKGAKGEVTAPGVEVSLPSVEVDAQVPGAKLEGDASLGDKEVAARDSKLKMPKFKMPSFGVSAAGKDLGTSVDVPAPKVGGEATLPSVGAELTAPEGSVPVPSAEVRLPGGELGVALPEGEAAGAELKGKAEGARIKAHLPKVQMPSIKMPKVDIRAPQVDIKGPSADLKGLKGEATAPGVEVSLPSVEVDAQVPGAKLEGDASLGDKEVAARDSKFKMPKFKMPSFGVSAPSKSVEASLDASLPKVQADVSLPSIQADVKTADLSVELPSAELEVKTAGAGVKLPEGPGPEGEPQEPAAGAGLKGHLPKVQMPSIKMPKVDFKFKMPSFGVSAPSKSVEASLDASLPKVQADVSLPSIQADVKTADLSVELPSAELEVKTAGAGVKLPEGPGPEGEPQEPAAGAGLKGHLPKVQMPSIKMPKVDFKAPQVDIKGPKLDVKGAKGEVTAPGVEVSLPSVEVDAQVPGAKLEGDASLGDKEVAARDSKLKMPKFKMPSFGVSAAGKDLGTSVDVPAPKVGGEATLPSVGAELTAPEGSVPVPSAEVRLPGGELGVALPEGEAAGAELKGKAEGARIKAHLPKVQMPSIKMPKVDIRAPQVDIKGPSADLKGLKGEATAPGVEVSLPSVEVDAQVPGAKLEGDASLGDKEVAAARDSKFKMPKFKMPSFGVSAPSKSVEASLDASLPKVQADVSLPSIQADVKTADLSVELPSAELEVKTAGAGVKLPEGPGPEGEPQEPAAGAGLKGHLPKVQMPSIKMPKVDFKAPQVDIKGPKLDVKGAKGEVTAPGVEVSLPSVEVDAQVPGAKLEGDASLGDKEVAARDSKLKMPKFKMPSFGVSAAGKDLGTSVDVPAPKVGGEATLPSVGAELTAPEGSVPVPSAEVRLPGGELGVALPEGEAAGAELKGKAEGARIKAHLPKVQMPSIKMPQVDIRAPQVDIKGPSADLKGLKGEATAPGVEVSLPSVEVDAQVPGAKLEGDASLGDKEVAARDSKFKMPKFKMPSFGVSAPSKSVEASLDASLPKVQADVSLPSIQADVKTADLSVELPSAELEVKTAGAGVKLPEGPGPEGEPQEPAAGAGLKGHLPKVQMPSIKMPKVDFKAPQVDIKGPKLDVKGAKGEVTAPGVEVSLPSVEVDAQVPGAKLEGDASLGDKEVAARDSKFKMPKFKMPSFGVSAPSKSVEASLDASLPKVQADVSLPSIQADVKTADLSVELPSAELEVKTAGAGVKLPEGPGPEGEPQEPAAGAGLKGHLPKVQMPSIKMPKVDFKAPQVDIKGPKLDVKGAKGEVTAPGVEVSLPSVEVDAQVPGAKLEGDASLGDKEVAARDSKLKMPKFKMPSFGVSAAGKDLGTSVDVPAPKVGGEATLPSVGAELTAPEGSVPVPSAEVRLPGGELGVALPEGEAAGAELKGKAEGARIKAHLPKVQMPSIKMPQVDIRAPQVDIKGPSADLKGLKGEATAPGVEVSLPSVEVDAQVPGAKLEGDASLGDKEVAARDSKFKMPKFKMPSFGVSAPSKSVEASLDASLPKVQADVSLPSIQADVKTADLSVELPSAELEVKTAGAGVKLPEGPGPEGEPQEPAAGAGLKGHLPKVQMPSIKMPKVDFKAPQVDIKGPKLDVKGAKGEVTAPGVEVSLPSVEVDAQVPGAKLEGDASLGDKEVAARDSKLKMPKFKMPSFGVSAAGKDLGTDMFPEVCAGDPSVSGVGFDGSVSVEKCAEKVKSRKSHFKFPKLFSPPERAPGDSLALDTDSAHPLPPSVSVPASEVPGSRSSLQRSRLGWAAFPSAKLELSGPQAEVPTLTECEGVTLTKYQVTVPRAAVPLDVPQEALSGSQVGAHLPSMESSADFPSPERVSLSPTHGHPGPVDPLFPASYGQVTFPIFHRPKFGFSVPKAADAEVEPRGVECAPTLSPLSPTPGLDSSAAEAAVFPLPGTHLPSLEVSVSQGPEGPQDTVETSAMAPGRAPAEEADCEGKSSPLKMPRLKLPSLRWSPKKGAEPKEDPGRSLEDEELSVVVDVGPTGVPPEVPVPGMEAHRDMPPEEDGEEKRAKTPGFGNLKLALPRMKTSKAGADLPQGRPDPSLSSPPGGVGGTSVMVGLPTEAPPQPSCRAPDAEASTAGSPQGDAAAGEVPATSQESWFRMPALHLPSFLRSSKDRGGAGGPGAQMPVPAASTSREGEAPATVKSQGAPVSWSEVEAPTSLQSPEGGADSAAAESAPHTDVLRCSLDSRGLKLDLPADSVSTGMTPLPGPAGPAGAGEGAGERSSHPEGPLRLKASSTDVPSQISVVTMGQLWEDSVQTVRFPKLEVPRFCFPAPSSEADVFVPTVREVQCSESSLDFALSTESPGDWGASILKTGAGVPGEQPVALDLSSEAPPISKVRVHIQGTEVESQEVTIHSRVTAEFAHLLGPEAISTQIVRESDIPASQIRTPSYGFSLLKAKIPEPPTQAGLHMVTRDSRLSEDLREAPRQVTPGADPISGDLQPDTGEPFEIISPGVSVPELQMCAFDVRPGHQFADSCSDEEPAEILEFPPEDSQEAPLADEDGAPKEKPESKRSAGLFRFWLPNIGFSSSVDETSAEPKDDVPKSAPIQTQPEAQPEAGPPKKQEKAGWFRFPKLGFSSTPTKKSKTPKDEAGPAEQNLQEEAVTFFDARETFSPEEKDGEPAEASGAGPGSGAMVASAARTELILLEQDREASEEPAPGPATE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IKH8</accession>
    <name>A0A9L0IKH8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="10">LSM2 homolog, U6 small nuclear RNA and mRNA degradation associated</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">LSM2</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the snRNP Sm proteins family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IKH8"/>
    <dbReference type="Ensembl" id="ENSEAST00005041258.1">
        <property type="protein sequence ID" value="ENSEASP00005041398.1"/>
        <property type="gene ID" value="ENSEASG00005029150.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000016597"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990726">
        <property type="term" value="C:Lsm1-7-Pat1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046540">
        <property type="term" value="C:U4/U6 x U5 tri-snRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01725">
        <property type="entry name" value="LSm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016654">
        <property type="entry name" value="U6_snRNA_Lsm2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13829">
        <property type="entry name" value="SNRNP CORE PROTEIN FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13829:SF2">
        <property type="entry name" value="U6 SNRNA-ASSOCIATED SM-LIKE PROTEIN LSM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
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                <person name="Guo"/>
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                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
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                <person name="Guo"/>
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                <person name="Yang"/>
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                <person name="Tian"/>
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                <consortium name="Ensembl"/>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
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                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="Zhou"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005044563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="247" mass="25860" checksum="7FAB70DCF73731A0" modified="2023-09-13" version="1">MAQPLCPPRPEAWMLSAGWGPARPPRPSDGDCSCSPASSSHSWASAANGSAVPSPGRPAPPAARAPTAGSGQRQSASEREKLRMRTLARALHELRRFLPPAVAPAGQSLTKIETLRLAIRYIGHLSAVLGLSEDSLRRRRPRPSASPRPRGCPPLPRRRGPAQVQTQTQTRGLGLGSAAGAAVSWGSPPADPGAPGAASEPCDPLELHNEAAGPQALAMEPGPSSLLFPDEVLALLETWMPLEWPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IZI8</accession>
    <name>A0A9L0IZI8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome c oxidase copper chaperone</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">COX17</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Copper metallochaperone essential for the assembly of the mitochondrial respiratory chain complex IV (CIV), also known as cytochrome c oxidase. Binds two copper ions and delivers them to the metallochaperone SCO1 which transports the copper ions to the Cu(A) site on the cytochrome c oxidase subunit II (MT-CO2/COX2).</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Interacts with COA1. Interacts with the chaperone CHCHD4; this is important for correct folding and the formation of disulfide bonds that stabilize the structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the COX17 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014693644.1">
        <property type="nucleotide sequence ID" value="XM_014838158.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014693645.1">
        <property type="nucleotide sequence ID" value="XM_014838159.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IZI8"/>
    <dbReference type="SMR" id="A0A9L0IZI8"/>
    <dbReference type="Ensembl" id="ENSEAST00005060155.1">
        <property type="protein sequence ID" value="ENSEASP00005045538.1"/>
        <property type="gene ID" value="ENSEASG00005038393.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106829186"/>
    <dbReference type="KEGG" id="eai:106829186"/>
    <dbReference type="CTD" id="10063"/>
    <dbReference type="GeneTree" id="ENSGT00390000002329"/>
    <dbReference type="OMA" id="QEACTKW"/>
    <dbReference type="OrthoDB" id="1915887at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016531">
        <property type="term" value="F:copper chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903136">
        <property type="term" value="F:cuprous ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008284">
        <property type="term" value="P:positive regulation of cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1130:FF:000001">
        <property type="entry name" value="cytochrome c oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1130">
        <property type="entry name" value="CytochromE C oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007745">
        <property type="entry name" value="Cyt_c_oxidase_Cu-chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05051">
        <property type="entry name" value="COX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51808">
        <property type="entry name" value="CHCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7 14" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00055863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00065132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00071062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR607745-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005045538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="63" mass="6851" checksum="7273DF70AEE144E9" modified="2023-09-13" version="1">MPGLTAQGPAPSDSQEKKPLKPCCACPETKKARDACIMEKGEEHCGHLIEAHKECMRALGFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IZY1</accession>
    <name>A0A9L0IZY1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ferritin heavy chain</fullName>
            <ecNumber evidence="4">1.16.3.1</ecNumber>
        </recommendedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Delivery to lysosomes is mediated by the cargo receptor NCOA4 for autophagic degradation and release of iron.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>4 Fe(2+) + O2 + 4 H(+) = 4 Fe(3+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:11148"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="EC" id="1.16.3.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited. Interacts with NCOA4; NCOA4 promotes targeting of the iron-binding ferritin complex to autolysosomes following starvation or iron depletion.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Lysosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ferritin family.</text>
    </comment>
    <dbReference type="EC" id="1.16.3.1" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0IZY1"/>
    <dbReference type="Ensembl" id="ENSEAST00005069270.1">
        <property type="protein sequence ID" value="ENSEASP00005043327.1"/>
        <property type="gene ID" value="ENSEASG00005037310.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000182841"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005764">
        <property type="term" value="C:lysosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431:SF37">
        <property type="entry name" value="FERRITIN HEAVY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0458">Lysosome</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin/DPS" evidence="11">
        <location>
            <begin position="19"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00039731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601519-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005043327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="71" mass="8365" checksum="919CE7E37DA85871" modified="2023-09-13" version="1">MTTAFPSQVRQNYHQDSEAAINRQINLELHASYVYLSMSFYFDQTATCMCACIHICTYIWKHIHIDLFCIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J0R8</accession>
    <name>A0A9L0J0R8_EQUAS</name>
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        <recommendedName>
            <fullName evidence="10">RAB11-binding protein RELCH</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">LisH domain and HEAT repeat-containing protein KIAA1468</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">RAB11-binding protein containing LisH, coiled-coil, and HEAT repeats</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">RELCH</name>
    </gene>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="9">Regulates intracellular cholesterol distribution from recycling endosomes to the trans-Golgi network through interactions with RAB11 and OSBP. Functions in membrane tethering and promotes OSBP-mediated cholesterol transfer between RAB11-bound recycling endosomes and OSBP-bound Golgi-like membranes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Recycling endosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J0R8"/>
    <dbReference type="Ensembl" id="ENSEAST00005056848.1">
        <property type="protein sequence ID" value="ENSEASP00005043607.1"/>
        <property type="gene ID" value="ENSEASG00005002668.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000004385"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032367">
        <property type="term" value="P:intracellular cholesterol transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000080">
        <property type="entry name" value="lisH domain and HEAT repeat-containing protein KIAA1468 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000218">
        <property type="entry name" value="lisH domain and HEAT repeat-containing protein KIAA1468 homolog isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040362">
        <property type="entry name" value="RELCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32059">
        <property type="entry name" value="RAB11-BINDING PROTEIN RELCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32059:SF0">
        <property type="entry name" value="RAB11-BINDING PROTEIN RELCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00667">
        <property type="entry name" value="LisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0967">Endosome</keyword>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="HEAT" evidence="13">
        <location>
            <begin position="1005"/>
            <end position="1043"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="155"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="407"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="198"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="360"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="7"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="15">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="15">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="422"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="432"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="467"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00058592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00081570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00084053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005043607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1217" mass="134528" checksum="1892826001DA3C87" modified="2023-09-13" version="1">MAAMAPGGSGSGGGGVNPFLSDSDEDDDEVAATEERRAGLRLGSGGGLDPGSADSLSPQDPVALGSSARSGLPGEAAAAAVALGGGGETPGRLSIDAIAAQLLRDQYLLTALELHTELLESGRELPRLRDYFSNPGNFERQSGTPPGMGAPGIPGAAGVGGAGGREPSTTSGGGQLNRAGSISTLDSLDFARYSDDGNRETDERVAVLEFELRKAKETIQALRANLTKAAEHEVPLQERKNYKSSPEIQEPIKPLEKRALNFLVNEFLLKNNYKLTSITFSDENDDQDFELWDDVGLNIPKPPDLLQLYRDFGNHQVTGKDLVDVASGVEEDELEALTPILGNLPPTLEASQTVQNSLLVQKLEDKISLLNSEKWSLMEQIGRLESEMNFLKNEHFAISAICDSVQPSLDQSSHKDSEDSEQNPVTNSSDKGQNKDIHLSKSDEVDSTIPKENFPNSFPRREREGMPSSSPSSKNTVHFDKPNRKLSPAFHQALLSFCRMSADSRLGSEVSRIADSEKSVMLMLGRCLPHIVPNVLLAKREELIPLILCTACLHPEPKERDQLLHILFNLIKRPDDEQRQMILTGCVAFARHVGPTRVEAELLPQCWEQINHKYPERRLLVAESCGALAPYLPKEIRSSLVLSMLQQMLMEDKADLVREAVIKSLGIIMGYIDDPDKYQQGFELLLSALGDPSERVVSATHQVFLPAYAAWTTELGNLQSHLIPTLLNKIEKLLREGEHGLDEHKLHMYLSALQSLIPSLFALVLQNAPFSSKAKLHGEVPQIEVTRFPRPMSPLQDVSTIIGSREQLAVLLQLYDHQLEHEGTTGWESLLWVVNQLLPQLIEIVGKINVTSTACVHEFSRFFWRLCRTFGKIFTNTKVKPQFQEILRLSEENIDSSAGNGVLTKATVPIYATGVLTCYIQEEDRKLLVGFLEDVMTLLSLSHAPLDSLKASFVELGANPAYHELLLTVLWYGVVHTSALVRCTAARMFELTLRGMSEALVDKRVAPALVTLSSDPEFSVRIATIPAFGTIMETVIQRELLERVKMQLASFLEDPQYQDQYSLHTEIIKTFGRVGPNAEPRFRDEFVIPHLHKLALVNNLQIVDSKRLDIATHLFEAYSALSCCFISEDLMVNHFLPGLRCLRIDMEQLSPEHEVILTSMIKECEQKVENKTVQEPQGSMSIAASLVSEDTKTKFLNKMGQLTTSGAMLANVFQRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J552</accession>
    <name>A0A9L0J552_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">G-protein coupled receptor 26</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">GPR26</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
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                <person name="Y."/>
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                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
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                <person name="A."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="entry name" value="NEFA-INTERACTING NUCLEAR PROTEIN NIP30"/>
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                <person name="X. and Zhong"/>
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="match status" value="1"/>
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            <end position="59"/>
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    <feature type="domain" description="SPATA31-like" evidence="8">
        <location>
            <begin position="70"/>
            <end position="155"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="6">
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            <end position="255"/>
        </location>
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        <location>
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    <feature type="compositionally biased region" description="Pro residues" evidence="6">
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        </location>
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    <feature type="compositionally biased region" description="Pro residues" evidence="6">
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    <feature type="compositionally biased region" description="Pro residues" evidence="6">
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        </location>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF15371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="380" mass="41394" checksum="40E1FFF9E6ABC37F" modified="2023-09-13" version="1">MEFSQWNVLSFLNSRIELFLSICSTFLDSDHSLTIVCGLWLLLLFLCFLVGIPSLPTFWKTKIYQKRQGRAKRRRKGGTPSGWRNYQREREEKRRLISILKRPLGRPLDTTRFRQLLCPDPSCEVCNSTTAEINRLLEDLEDDTASVSSLASTASGTESSFSLSSAFSEVPPGDLTPSPPPDPSPPPPSVLSPNPTTSLAGFLSPSPPGHSMPPEPSPPLESKFPADHCPPQPLALPPLPAHDTQATGPILQPEATLSPNTIFSLDRTLSQDINPLPNLSQIINPTDSLACHHTPPSLSVSPPTDHPLTATRSKSVSILLKSVPENSSPDRPGGLSTYVPTVRGTDHSSLSISELSWWQACAKDLFLAPSTLAPCAFNRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JFN9</accession>
    <name>A0A9L0JFN9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Double C2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106833483</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JFN9"/>
    <dbReference type="Ensembl" id="ENSEAST00005050946.1">
        <property type="protein sequence ID" value="ENSEASP00005052136.1"/>
        <property type="gene ID" value="ENSEASG00005005966.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162875"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098793">
        <property type="term" value="C:presynapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017158">
        <property type="term" value="P:regulation of calcium ion-dependent exocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061669">
        <property type="term" value="P:spontaneous neurotransmitter secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04035">
        <property type="entry name" value="C2A_Rabphilin_Doc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000032">
        <property type="entry name" value="Double c2-like domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000023">
        <property type="entry name" value="Double C2-like domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
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        <property type="entry name" value="C2_domain_sf"/>
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        <property type="entry name" value="Doc2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043566">
        <property type="entry name" value="Rabphilin/DOC2/Noc2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047022">
        <property type="entry name" value="Rabphilin_Doc2_C2A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001565">
        <property type="entry name" value="Synaptotagmin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45729:SF7">
        <property type="entry name" value="DOUBLE C2-LIKE DOMAIN-CONTAINING PROTEIN GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45729">
        <property type="entry name" value="RABPHILIN, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036931">
        <property type="entry name" value="Doc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00360">
        <property type="entry name" value="C2DOMAIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00399">
        <property type="entry name" value="SYNAPTOTAGMN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="C2" evidence="6">
        <location>
            <begin position="85"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="6">
        <location>
            <begin position="245"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="54"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR036931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="389" mass="43288" checksum="780395291D8EE258" modified="2023-09-13" version="1">MAGTAAASRGRPQRVSMQEHMAIDVSPGPIRPIHLISDFFPHFYPFAGPTLRAPGPRPAVTPALCSAPQPQPDPEPEGDSDDSTALGTLEFTLLFDADNSALHCTAHRAKGLKPPASGSVDTYVKANLLPGTSKASQLRTRTVRGTRGPVWEETLTYHGFTRQDAGRKTLRLCVCEDPRLRRRRRAPPLGELRVPLRKLVPNRARSFDVCLEKRRLTKRPKSLDTARGMSLYEEEEVEAEVAGEERGRILLSLCYSSQRGGLLVGVLRCAHLAPMDANGYSDPFVRLFLHPNVGKKSKYKTSVRRKTLNPEFNEEFFYAGPREELAQKTLLVSVWDYDLGTADDFIGRVQLSSRASGEGQQHWSECLGHSDRRLELWHPLDGAPFQLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JHI2</accession>
    <name>A0A9L0JHI2_EQUAS</name>
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        <recommendedName>
            <fullName evidence="6">PBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TALE/PBX homeobox family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JHI2"/>
    <dbReference type="Ensembl" id="ENSEAST00005083454.1">
        <property type="protein sequence ID" value="ENSEASP00005051808.1"/>
        <property type="gene ID" value="ENSEASG00005033171.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162643"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008422">
        <property type="entry name" value="KN_HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005542">
        <property type="entry name" value="PBX_PBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050224">
        <property type="entry name" value="TALE_homeobox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
        <property type="entry name" value="HOMEOBOX PROTEIN TRANSCRIPTION FACTORS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05920">
        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51978">
        <property type="entry name" value="PBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0371">Homeobox</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PBC" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005051808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="126" mass="14445" checksum="DE671C70DFBF90C5" modified="2023-09-13" version="1">MQLKQSTCEAVMTLCSQFLDARRNWWNFSKQATQVLNEYFYSHLSNPYRSKEAKEELARKGGITVSQAAQCFVGSNPGRGHGTAHQTTLRQHPTCQNLKDSQRRIYNYVLGGFGEKKEKNKISEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JIB5</accession>
    <name>A0A9L0JIB5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sperm equatorial segment protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SPESP1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in fertilization ability of sperm.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Secretory vesicle</location>
            <location evidence="2">Acrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the SPESP1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JIB5"/>
    <dbReference type="Ensembl" id="ENSEAST00005073341.1">
        <property type="protein sequence ID" value="ENSEASP00005052939.1"/>
        <property type="gene ID" value="ENSEASG00005028304.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000005362"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001669">
        <property type="term" value="C:acrosomal vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045171">
        <property type="term" value="C:intercellular bridge"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007340">
        <property type="term" value="P:acrosome reaction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007342">
        <property type="term" value="P:fusion of sperm to egg plasma membrane involved in single fertilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026743">
        <property type="entry name" value="Equatorial_segment"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31667">
        <property type="entry name" value="SPERM EQUATORIAL SEGMENT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31667:SF2">
        <property type="entry name" value="SPERM EQUATORIAL SEGMENT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15754">
        <property type="entry name" value="SPESP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="4" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="29"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="118"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005052939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="341" mass="38311" checksum="322CB304FE88D018" modified="2023-09-13" version="1">MTVTPDEEQNLNHYVQVLQNLILSVPTREPIRGRKSKSPNDIYSRGSKVSQVEETVTHGGASTENDVLINPISEEPTTFPTSGFTLGIGKKKDTESTAFWSIKPNNVSVVLHAEEPYIEKEEPEPEPVLKEKEAPKPFPDVTQLSPSPDVTSGKPFLTSSGRSTDWDTGAETEDVPQLSGEYEMDKPEALTFDKHLNNDDILKKISDINSEVQQVPLAESLKPEYREDIQASRDHLKRSLALAAAAEHKLKNMYKSQMSPVGRSSSEIDDIETVINMLYNSRSKLPEYLDIKYVPPEMRRRATTVFNTLKKILCVSRAETQNLIRKLLNNNIKILNLLDIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JLV9</accession>
    <name>A0A9L0JLV9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Interferon-induced transmembrane protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LOC106825562</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CD225/Dispanin family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014688308.2">
        <property type="nucleotide sequence ID" value="XM_014832822.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070344657.1">
        <property type="nucleotide sequence ID" value="XM_070488556.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070344658.1">
        <property type="nucleotide sequence ID" value="XM_070488557.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070344659.1">
        <property type="nucleotide sequence ID" value="XM_070488558.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070344660.1">
        <property type="nucleotide sequence ID" value="XM_070488559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JLV9"/>
    <dbReference type="Ensembl" id="ENSEAST00005037951.1">
        <property type="protein sequence ID" value="ENSEASP00005054209.1"/>
        <property type="gene ID" value="ENSEASG00005029736.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106825562"/>
    <dbReference type="KEGG" id="eai:106825562"/>
    <dbReference type="GeneTree" id="ENSGT00950000182857"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046597">
        <property type="term" value="P:host-mediated suppression of symbiont invasion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045071">
        <property type="term" value="P:negative regulation of viral genome replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035455">
        <property type="term" value="P:response to interferon-alpha"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035456">
        <property type="term" value="P:response to interferon-beta"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034341">
        <property type="term" value="P:response to type II interferon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060337">
        <property type="term" value="P:type I interferon-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007593">
        <property type="entry name" value="CD225/Dispanin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051517">
        <property type="entry name" value="IFITM_antiviral_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13999">
        <property type="entry name" value="INTERFERON INDUCIBLE TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13999:SF4">
        <property type="entry name" value="INTERFERON-INDUCED TRANSMEMBRANE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04505">
        <property type="entry name" value="CD225"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="108"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005054209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="147" mass="16230" checksum="59EA8379FB8FBF1F" modified="2023-09-13" version="1">MNRTFQTFVSGAHTGVPPTYEMLKEEHEVSVLGAPQSSAPVTTTVINIHSETSVPDHIVWSLFNTLFMNWCCLGFVAFAYSVKSRDRKMVGDVTGAQSYASTAKCLNIWALVLGLFLSTGFIVLMGFTCLTLYQIIVKAMQDGRGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JLW7</accession>
    <name>A0A9L0JLW7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">F-box only protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">FBXO3</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of a SCF (SKP1-cullin-F-box) protein ligase complex SCF(FBXO3) consisting of FBXO3, SKP1, CUL1 and RBX1. Interacts with PML, interaction is direct and takes place either alone or within the SCF complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="F-box only protein 3"/>
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        <property type="entry name" value="ApaG domain"/>
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        <property type="entry name" value="SMI1/KNR4-like"/>
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        <property type="entry name" value="F-box_SCF_Substrate_Recog"/>
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    </dbReference>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0833">Ubl conjugation pathway</keyword>
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        <location>
            <begin position="165"/>
            <end position="295"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="306"/>
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        <location>
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    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Li"/>
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                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="Guo"/>
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                <person name="Pan"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Liu"/>
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                <person name="Jordana"/>
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                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="13" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045820">
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:neuron development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090063">
        <property type="term" value="P:positive regulation of microtubule nucleation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099171">
        <property type="term" value="P:presynaptic modulation of chemical synaptic transmission"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032465">
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0008277">
        <property type="term" value="P:regulation of G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="term" value="P:regulation of synaptic vesicle exocytosis"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:synaptic vesicle recycling"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="ARF GTPASE-ACTIVATING PROTEIN GIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46097">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR KINASE INTERACTING ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ank_2"/>
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    </dbReference>
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    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 9" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="1"/>
            <end position="124"/>
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            <begin position="166"/>
            <end position="198"/>
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            <end position="615"/>
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        <location>
            <begin position="449"/>
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            <begin position="366"/>
            <end position="383"/>
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        <location>
            <begin position="394"/>
            <end position="403"/>
        </location>
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    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="483"/>
            <end position="493"/>
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            <dbReference type="ARBA" id="ARBA00022468"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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            <dbReference type="SAM" id="Coils"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
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            <dbReference type="PROSITE" id="PS50115"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JMN4</accession>
    <name>A0A9L0JMN4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">COMM domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">COMMD3</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the COMM domain-containing protein 3 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JMN4"/>
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        <property type="protein sequence ID" value="ENSEASP00005054484.1"/>
        <property type="gene ID" value="ENSEASG00005006975.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015971"/>
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        <property type="component" value="Chromosome 29"/>
    </dbReference>
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        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04751">
        <property type="entry name" value="Commd3"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="COMM"/>
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    <dbReference type="InterPro" id="IPR037355">
        <property type="entry name" value="COMMD3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31159">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31159:SF1">
        <property type="entry name" value="COMM DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="COMM_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="COMM_HN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51269">
        <property type="entry name" value="COMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="COMM" evidence="4">
        <location>
            <begin position="389"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="90"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00093469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="460" mass="51028" checksum="189D0378B01472F4" modified="2023-09-13" version="1">MRTIGKCVCVYTHTPPHAGRNQLPYSRNDNEKGKINNYIIRGLQFHSLLGEGEWFKLLSEKKKNPNPKKQPVALNWSVPQAFALSEERGRAVFGGGGGGDRDSFQHLPRGSWRGRSRGAPQTLVTVPLGARWRRKHRARPQSARGRPCRGGAAAPAAGAEGNAGRKRRRGRRGGGGRRRGGRLGARAESQRGGLGRGGPQPAPPPGRRARAADQLSARRASRGARVEGHGALTMELSEFVQKGVQRLADPGSFDSNAFTLLLRAAFQSLLDAQADEAVLDHPDLKHIDPVVLKHCHAAAATYILEAAKQSADKSTLSTYLEDCKFDRERIELFCTEYQVTYLKFFKLTITIFLLLCLQRCVFFLSFFPPQNNKNSLEILLGSIGTSLPHITDVSWRLEYQIKTNQLQKMYRPAYSVTLNVENTDSQSHPEISFRCNMEQLQDLVGKLKDAAKSLERATQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JPR0</accession>
    <name>A0A9L0JPR0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tubulin polymerization-promoting protein family member 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TPPP3</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
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            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
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                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="Jin"/>
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                <person name="Yue"/>
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                <person name="Liu"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="term" value="P:embryo implantation"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="entry name" value="Tubulin polymerization-promoting protein family member 3"/>
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    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="TPP/p25"/>
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        <property type="entry name" value="P25 ALPHA-RELATED"/>
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        <property type="entry name" value="TUBULIN POLYMERIZATION-PROMOTING PROTEIN FAMILY MEMBER 3"/>
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    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005051967.1"/>
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    </evidence>
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        <source>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
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                <person name="Yin"/>
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                <person name="Jin"/>
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                <person name="Liu"/>
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                <person name="Jordana"/>
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                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <property type="term" value="F:microtubule binding"/>
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        <property type="project" value="InterPro"/>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005055073.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="99" mass="10474" checksum="BF5D4A66944DA544" modified="2023-09-13" version="1">MADLSLADALTDPPPEIEEEIKRDFIATLEAEAFDDVVGETVGKTDYIPLLDVDEKTGNSESKKKPCSDASQVEGTPSSKPAVLANGDHGIEGNDTIEA</sequence>
</entry>
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            <fullName evidence="8">39S ribosomal protein L51, mitochondrial</fullName>
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    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mL51 family.</text>
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                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="G."/>
                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="J."/>
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                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
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                <person name="Yue"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <text evidence="1">Odorant receptor.</text>
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    <comment type="function">
        <text evidence="2">Putative odorant or sperm cell receptor.</text>
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        <property type="entry name" value="7tmA_OR13-like"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </evidence>
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        <source>
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                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Guo"/>
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                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Li"/>
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                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
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                <consortium name="Ensembl"/>
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        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Mitochondrial NAD(+) transporter SLC25A47</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SLC25A47</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Mitochondrial NAD(+) transporter that acts as a 'metabolic gate' in hepatic lipogenesis. Provides NAD(+) substrate to mitochondrial SIRT3 deacetylase and enables its NAD(+)-dependent activities in mitochondrial energy metabolism. This triggers downstream activation of PRKAA1/AMPK-alpha signaling cascade that negatively regulates sterol regulatory element-binding protein (SREBP) transcriptional activities and ATP-consuming lipogenesis to restore cellular energy balance. May transport other mitochondrial metabolites having an aromatic nucleotide and phosphate groups, such as acetyl-CoA. Does not transport amino acids. The transport mechanism remains to be elucidated.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>NAD(+)(in) = NAD(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:65408"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>acetyl-CoA(in) = acetyl-CoA(out)</text>
            <dbReference type="Rhea" id="RHEA:75039"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 18">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014710052.2">
        <property type="nucleotide sequence ID" value="XM_014854566.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JVZ0"/>
    <dbReference type="Ensembl" id="ENSEAST00005038529.1">
        <property type="protein sequence ID" value="ENSEASP00005054197.1"/>
        <property type="gene ID" value="ENSEASG00005021609.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106839660"/>
    <dbReference type="KEGG" id="eai:106839660"/>
    <dbReference type="CTD" id="283600"/>
    <dbReference type="GeneTree" id="ENSGT00940000159694"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000069">
        <property type="entry name" value="Solute carrier family 25 member 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.40.10:FF:000072">
        <property type="entry name" value="solute carrier family 25 member 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002067">
        <property type="entry name" value="MCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF3">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 25 MEMBER 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00926">
        <property type="entry name" value="MITOCARRIER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 17 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 18" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="17">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="17">
        <location>
            <begin position="93"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="17">
        <location>
            <begin position="217"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="123"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="123"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00050469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00052765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00053322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00070489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00081036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005054197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="310" mass="33759" checksum="35C606AB42103556" modified="2023-09-13" version="1">MDFVAGAIGGVCGVAVGYPLDTVKVRIQTEPRYTGIWHCVWDTYRRERVWGFYRGLSLPVCTVSLVSSLSFGTYRHCLSHICRFRYGSADAKPAKTDITLSGFASGLVRVFLTSPTEVAKVRLQTQTQPQPQQRRLSTSGPSAVPSVCPAPPGRPVPGPKYHGPLHCLATVAREEGLRGLYRGSSALLFRDGHSFATYFLSYALLCEWLTPAGHSQPDVLGVLVAGGCAGVLAWAVATPMDVIKSRLQVDGQGQRRYRGLLHCMAASVREEGPRVLFKGLTLNCCRAFPVNMAVFVTYEAVLRLARGLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JW37</accession>
    <name>A0A9L0JW37_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetyl-CoA carboxylase 1</fullName>
            <ecNumber evidence="4">6.4.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="23">ACC-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="31" type="primary">ACACA</name>
    </gene>
    <organism evidence="31 32">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="31 32" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="31" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="31" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="20">Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis. This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>hydrogencarbonate + acetyl-CoA + ATP = malonyl-CoA + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11308"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="22">
            <dbReference type="Rhea" id="RHEA:11309"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Monomer, homodimer, and homotetramer. Can form filamentous polymers. Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. Interacts with MID1IP1; interaction with MID1IP1 promotes oligomerization and increases its activity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="6.4.1.2" evidence="4"/>
    <dbReference type="RefSeq" id="XP_070338937.1">
        <property type="nucleotide sequence ID" value="XM_070482836.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005078018.1">
        <property type="protein sequence ID" value="ENSEASP00005057511.1"/>
        <property type="gene ID" value="ENSEASG00005000212.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106844682"/>
    <dbReference type="CTD" id="31"/>
    <dbReference type="GeneTree" id="ENSGT00940000156706"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003989">
        <property type="term" value="F:acetyl-CoA carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071380">
        <property type="term" value="P:cellular response to prostaglandin E stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055088">
        <property type="term" value="P:lipid homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051289">
        <property type="term" value="P:protein homotetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001894">
        <property type="term" value="P:tissue homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.460.10:FF:000001">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000005">
        <property type="entry name" value="Acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1770.10:FF:000001">
        <property type="entry name" value="acetyl-CoA carboxylase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000005">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000010">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.460.10">
        <property type="entry name" value="Biotin dependent carboxylase carboxyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1770.10">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049076">
        <property type="entry name" value="ACCA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049074">
        <property type="entry name" value="ACCA_BT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034733">
        <property type="entry name" value="AcCoA_carboxyl_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013537">
        <property type="entry name" value="AcCoA_COase_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011763">
        <property type="entry name" value="COA_CT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011762">
        <property type="entry name" value="COA_CT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728:SF5">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45728">
        <property type="entry name" value="ACETYL-COA CARBOXYLASE, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08326">
        <property type="entry name" value="ACC_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21385">
        <property type="entry name" value="ACCA_BT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01039">
        <property type="entry name" value="Carboxyl_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50989">
        <property type="entry name" value="COA_CT_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50980">
        <property type="entry name" value="COA_CT_NTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="13 24" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="18" id="KW-0092">Biotin</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="16" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0436">Ligase</keyword>
    <keyword evidence="7" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="17" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="19" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="11 24" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="32" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="25" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="25">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040305804" description="Acetyl-CoA carboxylase 1" evidence="25">
        <location>
            <begin position="22"/>
            <end position="2384"/>
        </location>
    </feature>
    <feature type="domain" description="Biotin carboxylation" evidence="28">
        <location>
            <begin position="155"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="27">
        <location>
            <begin position="313"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="26">
        <location>
            <begin position="783"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase N-terminal" evidence="29">
        <location>
            <begin position="1614"/>
            <end position="1952"/>
        </location>
    </feature>
    <feature type="domain" description="CoA carboxyltransferase C-terminal" evidence="30">
        <location>
            <begin position="1956"/>
            <end position="2272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00045132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00046553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00075764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS50980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="30">
        <source>
            <dbReference type="PROSITE" id="PS50989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005057511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="2384" mass="270222" checksum="1359E2AB45EAC8E1" modified="2023-09-13" version="1">MWWSTLMSILRASSFWRWISAQTERIIRALRAHFDRIMDEPSPLAKLALELNQHSRFIIGSVSEDNSEDEISSLVKLDLLEEKEGSLSPASVGSDALSDLGISSLQDGLALHMRSSMSGLHIVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRWSYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWGHASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWQENDFTKRILNVPQELYEKGYVKDVDDGLKAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMRLAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPAAIATPAVFEHMEQCAVKLAKMVGYVSAGTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGLSPWGDAPIDFENSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHCFSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQLNRIDTGWLDRLIAEKVQAERPDTMLGVVCGALHVADVSLRNSISNFLHSLERGQVLPAHTLLNTVDVELIYEGVKYVLKVTRQSPNSYVVVMNGSCVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVMRSPSAGKLIQYIVEDGGHVFAGQCYAEIEVMKMVMTLTAAESGCIHYVKRPGAALDPGCVIAKMQLDNPSKVQQAELHTGSLPRIHSTALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSRVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRIPPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIRGHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCGRDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENLQKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPNRGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFCDSPPQSPTFPEAGHTSLYDEDKVPRNEPIHILNVAIKTDGDIEDDRLAAMFREFTQQKKSTLVEHGIRRLTFLVAQKDFRKQVNYEVDQRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHKMHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDCNHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYLDISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLIKLWESMSTHAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTLKSPEYPEGRDIIVIGNDITYRIGSFGPQEDLLFLRASELARAEGIPRIFVAANSGARIGLAEEIRHMFHVAWVDPEDPYKGYKYLYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGLGVENLRSSGMIAGESSLAYDEIITISLVTCRAIGIGAYLVRLGQRTIQVENSHLILTGAAALNKVLGREVYTSNNQLGGIQIMHNNGVTHSKVCDDFEGVFTVLHWLSYMPKSVHSSVPLLNSKDPIDRIIEFVPTKAPYDPRWMLAGRPHPTQKGQWLSGFFDYGSFSEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAIKDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDGLRECSQPVMVYIPPQAELRGGSWVVIDPTINPRHMEMYADRESRGSILEPEGTVEIKFRRKDLVKTMRRVDPVYIHLAERLGTPELSAAERKELENKLKEREEFLIPIYHQVAVQFADLHDTPGRMQEKGIINDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELTDGQIQAMLRRWFVEVEGTVKAYVWDNNKDLVEWLEKQLTEEDGVRSVIEENIKYISRDYVLKQIRSLVQANPEVAMDSIIHMTQHISPTQRAEVVRILSTMDSPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JW59</accession>
    <name>A0A9L0JW59_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Proprotein convertase subtilisin/kexin type 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Proprotein convertase 5</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Proprotein convertase 6</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Subtilisin/kexin-like protease PC5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="29" type="primary">PCSK5</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="29 30" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="29" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="29" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Serine endoprotease that processes various proproteins by cleavage at paired basic amino acids, recognizing the RXXX[KR]R consensus motif. Likely functions in the constitutive and regulated secretory pathways. Plays an essential role in pregnancy establishment by proteolytic activation of a number of important factors such as BMP2, CALD1 and alpha-integrins. May be responsible for the maturation of gastrointestinal peptides. May be involved in the cellular proliferation of adrenal cortex via the activation of growth factors.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="16">Endomembrane system</location>
            <topology evidence="16">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 23 24">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070351425.1">
        <property type="nucleotide sequence ID" value="XM_070495324.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005066621.1">
        <property type="protein sequence ID" value="ENSEASP00005053365.1"/>
        <property type="gene ID" value="ENSEASG00005004745.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106827811"/>
    <dbReference type="CTD" id="5125"/>
    <dbReference type="GeneTree" id="ENSGT00940000155770"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007565">
        <property type="term" value="P:female pregnancy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00064">
        <property type="entry name" value="FU"/>
        <property type="match status" value="17"/>
    </dbReference>
    <dbReference type="CDD" id="cd04059">
        <property type="entry name" value="Peptidases_S8_Protein_convertases_Kexins_Furin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000011">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000018">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000033">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000035">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000037">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000040">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000042">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000044">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000049">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000057">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000076">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000006">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.850:FF:000001">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000002">
        <property type="entry name" value="Proprotein convertase subtilisin/kexin type 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.220.10:FF:000022">
        <property type="entry name" value="proprotein convertase subtilisin/kexin type 5 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.220.10">
        <property type="entry name" value="Hormone Receptor, Insulin-like Growth Factor Receptor 1, Chain A, domain 2"/>
        <property type="match status" value="15"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.850">
        <property type="entry name" value="Peptidase S8, pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006212">
        <property type="entry name" value="Furin_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032778">
        <property type="entry name" value="GF_recep_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009030">
        <property type="entry name" value="Growth_fac_rcpt_cys_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034182">
        <property type="entry name" value="Kexin/furin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002884">
        <property type="entry name" value="P_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032815">
        <property type="entry name" value="S8_pro-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038466">
        <property type="entry name" value="S8_pro-domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN/KEXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42884:SF7">
        <property type="entry name" value="PROPROTEIN CONVERTASE SUBTILISIN_KEXIN TYPE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14843">
        <property type="entry name" value="GF_recep_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01483">
        <property type="entry name" value="P_proprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16470">
        <property type="entry name" value="S8_pro-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00181">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SMART" id="SM01411">
        <property type="entry name" value="Ephrin_rec_like"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00261">
        <property type="entry name" value="FU"/>
        <property type="match status" value="22"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57184">
        <property type="entry name" value="Growth factor receptor domain"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51829">
        <property type="entry name" value="P_HOMO_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0165">Cleavage on pair of basic residues</keyword>
    <keyword evidence="15" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10 23 24" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0635">Pregnancy</keyword>
    <keyword evidence="4 23 24" id="KW-0645">Protease</keyword>
    <keyword evidence="30" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="11 23 24" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8 27" id="KW-0732">Signal</keyword>
    <keyword evidence="6 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 26" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="14" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="27">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040221181" description="Proprotein convertase subtilisin/kexin type 5" evidence="27">
        <location>
            <begin position="33"/>
            <end position="1859"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="1744"/>
            <end position="1764"/>
        </location>
    </feature>
    <feature type="domain" description="P/Homo B" evidence="28">
        <location>
            <begin position="461"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1786"/>
            <end position="1821"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="22 23">
        <location>
            <position position="171"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="22 23">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="22 23">
        <location>
            <position position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00053092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00069955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00076732"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00076945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00076948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS51829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005053365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1859" mass="206943" checksum="722D39864B3AD75E" modified="2023-09-13" version="1">MGWGSRCCCPGRLDLLCVLALLGGCLLPVCRTRVYTNHWAVKIAGGFPEANRIASKYGFINMGQIGALKDYYHFYHSRTIKRSVLSSRGTHSFISMEPKVEWIQQQVVKKRTKRDYDFSRAQSTYFNDPKWPSMWYMHCSDNTHPCQSDMNIEGAWKRGYTGKNIVVTILDDGIERTHPDLMQNYDALASCDVNGNDLDPMPRYDASNENKHGTRCAGEVAAAANNSHCTVGIAFNAKIGGVRMLDGDVTDMVEAKSVSFNPQHVHIYSASWGPDDDGKTVDGPAPLTRQAFENGVRMGRRGLGSVFVWASGNGGRSKDHCSCDGYTNSIYTISISSTAESGKKPWYLEECSSTLATTYSSGESYDKKIITTDLRQRCTDNHTGTSASAPMAAGIIALALEANPFLTWRDVQHVIVRTSRAGHLNANDWKTNAAGFKVSHLYGFGLMDAEAMVMEAEKWTTVPQQHVCVESTDRQIKTIRPNSAVRSIYKASGCSDNPNHHVNYLEHVVVRITITHPRRGDLAIYLTSPSGTRSQLLANRLFDHSMEGFKNWEFMTIHCWGERAAGDWILEVYDTPSQLRNFKTPGKLKEWSLVLYGTSVQPYSPTNEFPKVERVRYSRVEDPTDDYGTEDYPGPCDPECSEVGCDGPGPDHCNDCLHYYYKLKNNTRICVSSCPPGHYHADKKRCRKCAPNCEACFGSHGDQCLSCKYGYFLNEETNSCVTHCPDGSYQDSKKNLCRKCSENCKTCTESHNCTECRDGLSLQGSRCFVACEDGRYFNGQDCQPCHRFCATCAGAGADGCINCTDGYFMEDGRCVQSCSISYYFDQSSENGYKSCKKCDASCLTCNGPGFKNCTSCPSGYLLDLGMCQMGAICKDGEYIDEHGHCQICDASCAKCWGPTQEDCTGCPVTRIFDDGRCILNCPPGKFEFKSQCHPCHYTCRECRGNKPSNCTSCGVDKHGQERFLYQGECWESCPAGHYPAKGHACLPCPDNCELCHSAHICTQCVSGYFMVPTNHTCQKVECGPGEVQDPDYEECVPCEEGCLGCSLDDPGTCTSCAMGYYMFEHHCYKACPEKTYSEELECKACDTNCGSCDQNECYWCEEGFFLLGGSCVRNCGPGLHGDPEIGECEPCHRACKTCTGLAYDECSSCREGLWLLNGTCVSPTQTQVEGKFWNDIWRKDQPCHPSCKTCNGSETLCTSCHKGKYLLAQACVSSCPQGTWPSVRSGSCEICTEDCVSCSGANLCKECRTQPDQPLFLHEGRCYTKCPKGFYAADSTCERCSSPCRTCEGNATNCHSCEGGLVLHHGECRETCSERHVAVEGVCKHCPEMCQDCIHEKTCKECMPGSFLYEDMCHQSCPNHFYEDARQCVPCHEDCLECSGPSADDCDLCAEESLVLYNGRCLDECPEGTYYEKMIKVCKDCHKSCQICSPSGTCMTCQDGWRMNHHGDCVPHQECAPSEYWDEGALGCKPCHAKCFHCTGPSEDQCHTCLRDSLLLNTTCVQDCPEGYYADEDSHRCVPCHSSCRTCEGRYSMQCLSCQPGWFQLGKECLPQCREGYYAENATGRCERCNRSCKACRGPRPTDCLSCDPFFFLLRSKGECRRTCPEHYYAEQSAQTCERCHPTCDKCKGREALDCLSCVWSYHLVGGICASDCLVGEYRVGEGEKLNCEKCHESCIECKGPGAKNCTVCPANLVLHMDDGRCLRCCNASDPTDAQECCDCQGTMDECILRASEIGPAAERSKTALFITSSIMLVLLFGAAVMVWRKSRGRVQRAEKVRYEKLADPSKSYSSYKSSPRESTSFEEDQVIEYRDRDYDEDDDDDIVYMGQDGTVYRKFKYGLLNDDDDDELEYDDESYSYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JWG4</accession>
    <name>A0A9L0JWG4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Small integral membrane protein 8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SMIM8</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SMIM8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014703933.1">
        <property type="nucleotide sequence ID" value="XM_014848447.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JWG4"/>
    <dbReference type="Ensembl" id="ENSEAST00005077003.1">
        <property type="protein sequence ID" value="ENSEASP00005054382.1"/>
        <property type="gene ID" value="ENSEASG00005027622.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106835846"/>
    <dbReference type="KEGG" id="eai:106835846"/>
    <dbReference type="CTD" id="57150"/>
    <dbReference type="GeneTree" id="ENSGT00390000011674"/>
    <dbReference type="OMA" id="YMHATRE"/>
    <dbReference type="OrthoDB" id="1880105at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026686">
        <property type="entry name" value="UPF0708"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14274">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14274:SF1">
        <property type="entry name" value="SMALL INTEGRAL MEMBRANE PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14937">
        <property type="entry name" value="DUF4500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005054382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="97" mass="11031" checksum="B4C2426844436382" modified="2023-09-13" version="1">MSSAPEPPTFEKGPPKEKDFRNPGLRGVRTTTLFRAVNPELFIKPNKPVMAFGLITLSLCVAYIGYLHATQENKKELYEAVDSEGHSYMRRKTSKWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JZG9</accession>
    <name>A0A9L0JZG9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">BMP and activin membrane-bound inhibitor homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">BAMBI</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Negatively regulates TGF-beta signaling.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the BAMBI family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JZG9"/>
    <dbReference type="Ensembl" id="ENSEAST00005067984.1">
        <property type="protein sequence ID" value="ENSEASP00005055467.1"/>
        <property type="gene ID" value="ENSEASG00005029914.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154101"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 29"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005109">
        <property type="term" value="F:frizzled binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007179">
        <property type="term" value="P:transforming growth factor beta receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd23576">
        <property type="entry name" value="TFP_LU_ECD_BAMBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.60.10:FF:000018">
        <property type="entry name" value="BMP and activin membrane-bound inhibitor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.60.10">
        <property type="entry name" value="CD59"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045806">
        <property type="entry name" value="BAMBI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045807">
        <property type="entry name" value="BAMBI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045860">
        <property type="entry name" value="Snake_toxin-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15505:SF1">
        <property type="entry name" value="BMP AND ACTIVIN MEMBRANE-BOUND INHIBITOR HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15505">
        <property type="entry name" value="RIIA DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06211">
        <property type="entry name" value="BAMBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19337">
        <property type="entry name" value="BAMBI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57302">
        <property type="entry name" value="Snake toxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="337"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="BMP and activin membrane-bound inhibitor N-terminal" evidence="12">
        <location>
            <begin position="215"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="BMP and activin membrane-bound inhibitor C-terminal" evidence="13">
        <location>
            <begin position="313"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00061218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00073194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF06211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF19337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005055467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="449" mass="48094" checksum="DF14817917125E7F" modified="2023-09-13" version="1">RLELAAGAPCARSGASACAESGRPRALPGLRRRVERGAGGHRAGPRKSSGSRARPPAALHAPAAARPPRPVADGARRPGQHSCSARAGSRRLQLRRGSGSRRGRQWIAIPATSSSGCSSNCAPWPCCSPKVGARRPGAGSGEGRGAPGRPPPPRAFEAGCQGFVSAGRAFLPRWSPQVATSCLFFGVAAVASRPSPRLSRARRVEVASDLGASGCEIRCYCDAAHCVATGYMCKSELSACFSRLLDPQNTNSPLTHGCLDSLASTADICQAKQAHNHSGPTMPTLECCHEDMCNYRGLHDVLSPPKGDASGQRNRHQHDGSRNLITKVQELTSSKELWFRAAVIAVPIAGGLILVLLIMLALRMLRSENKRLHDQRQQMLSRLHYSFHGHHSKKGQVAKLDLECMVPVSGHENCCLTCDKMRQADLSNDKILSLVHWGMYSGHGKLEFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JZU1</accession>
    <name>A0A9L0JZU1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Regulator of G-protein signaling 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RGS4</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="6">Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(z)-alpha is inhibited by phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-alpha-1 is inhibited by palmitoylation of the G-protein.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014696657.2">
        <property type="nucleotide sequence ID" value="XM_014841171.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JZU1"/>
    <dbReference type="Ensembl" id="ENSEAST00005073054.1">
        <property type="protein sequence ID" value="ENSEASP00005054615.1"/>
        <property type="gene ID" value="ENSEASG00005031920.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106831140"/>
    <dbReference type="KEGG" id="eai:106831140"/>
    <dbReference type="CTD" id="5999"/>
    <dbReference type="GeneTree" id="ENSGT00940000159036"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08714">
        <property type="entry name" value="RGS_RGS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.196.10:FF:000001">
        <property type="entry name" value="Regulator of G-protein signaling 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.196.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034953">
        <property type="entry name" value="RGS_RGS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024066">
        <property type="entry name" value="RGS_subdom1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF184">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="4" id="KW-0564">Palmitate</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0734">Signal transduction inhibitor</keyword>
    <feature type="domain" description="RGS" evidence="8">
        <location>
            <begin position="62"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="180"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="180"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00020122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005054615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="205" mass="23259" checksum="680B5E20AC2F54A7" modified="2023-09-13" version="1">MCKGLAGLPASCLRSAKDMKHRLGFLLQKSDSCEHNSSHSKKDKVVICQRVSQEEVKKWAESLENLISHECGLAAFKAFLKSEYSEENIDFWISCEEYKKIKSPSKLSPKAKKIYNEFISVQATKEVNLDSCTREETSRNMLEPTITCFDEAQKKIFNLMEKDSYRRFLKSRFYLDLANPSSCGSEKQKGAKSSSDCPSLVPQCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K1L7</accession>
    <name>A0A9L0K1L7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="37">Cyclic GMP-AMP synthase</fullName>
            <ecNumber evidence="41">2.7.7.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="42">2'3'-cGAMP synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="46" type="primary">CGAS</name>
    </gene>
    <organism evidence="46 47">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="46 47" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="46" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="46" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="38">
            <text>GTP + ATP = 2',3'-cGAMP + 2 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:42064"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:143093"/>
            <dbReference type="EC" id="2.7.7.86"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="38">
            <dbReference type="Rhea" id="RHEA:42065"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="39">
            <text>GTP + ATP = pppGp(2'-5')A + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23748"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:78318"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="39">
            <dbReference type="Rhea" id="RHEA:23749"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="40">
            <text>pppGp(2'-5')A = 2',3'-cGAMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23924"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78318"/>
            <dbReference type="ChEBI" id="CHEBI:143093"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="40">
            <dbReference type="Rhea" id="RHEA:23925"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
            <location evidence="7">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the mab-21 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.86" evidence="41"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0K1L7"/>
    <dbReference type="Ensembl" id="ENSEAST00005070572.1">
        <property type="protein sequence ID" value="ENSEASP00005058658.1"/>
        <property type="gene ID" value="ENSEASG00005028339.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01050000244827"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Mab-21-like_nuc_Trfase"/>
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        <property type="entry name" value="Mab-21_HhH/H2TH-like"/>
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    <dbReference type="InterPro" id="IPR024810">
        <property type="entry name" value="MAB21L/cGLR"/>
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    <dbReference type="PANTHER" id="PTHR10656">
        <property type="entry name" value="CELL FATE DETERMINING PROTEIN MAB21-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10656:SF35">
        <property type="entry name" value="CYCLIC GMP-AMP SYNTHASE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Mab-21"/>
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        <property type="entry name" value="Mab-21_C"/>
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    </dbReference>
    <dbReference type="SMART" id="SM01265">
        <property type="entry name" value="Mab-21"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="27" id="KW-0007">Acetylation</keyword>
    <keyword evidence="21" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="28" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="23" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0158">Chromosome</keyword>
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    <keyword evidence="15" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="13" id="KW-1017">Isopeptide bond</keyword>
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    <keyword evidence="36" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="24" id="KW-0460">Magnesium</keyword>
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    <keyword evidence="11" id="KW-0488">Methylation</keyword>
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    <keyword evidence="25" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="22" id="KW-0862">Zinc</keyword>
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            <begin position="277"/>
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            <begin position="470"/>
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            <begin position="1"/>
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            <begin position="12"/>
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            <begin position="158"/>
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        </location>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K1S7</accession>
    <name>A0A9L0K1S7_EQUAS</name>
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            <fullName evidence="15">Ig-like domain-containing protein</fullName>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="12">Gamma-delta TR is a heterodimer composed of a gamma and delta chain; disulfide-linked. The gamma-delta TR is associated with the transmembrane signaling CD3 coreceptor proteins following the stoichiometry: a single gamma-delta TR heterodimer associates with one CD3D-CD3E heterodimer, one CD3G-CD3E heterodimer and one CD247 homodimer forming a stable octameric structure. Upon activation, gamma-delta TR complex associates with FCER1G to initiate intracellular signaling.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
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    <keyword evidence="10" id="KW-0675">Receptor</keyword>
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    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
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            <end position="153"/>
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            <end position="114"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00038578"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00043266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005056292.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="153" mass="17760" checksum="D84EC340F2C59EA4" modified="2023-09-13" version="1">MLWTLAFLLAFLAPATWISSNVEGTQTSITKQAGSSVVFTCDSKQRITYVHWYRHKEGMAPQRIVYYHHQDSRLVSDPGIRLERYRVYESTERNRKIEIRKLEQSDSGVYYCAIWEAHSDSHLPYSSLKILLVAATKDRPSPCLTYRPDFTVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K377</accession>
    <name>A0A9L0K377_EQUAS</name>
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        <recommendedName>
            <fullName evidence="13">Vomeronasal type-1 receptor</fullName>
        </recommendedName>
    </protein>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Putative pheromone receptor.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0007606">
        <property type="term" value="P:sensory perception of chemical stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd13949">
        <property type="entry name" value="7tm_V1R_pheromone"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Vomeronasal type-1 receptor"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004072">
        <property type="entry name" value="Vmron_rcpt_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24062">
        <property type="entry name" value="VOMERONASAL TYPE-1 RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03402">
        <property type="entry name" value="V1R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01534">
        <property type="entry name" value="VOMERONASL1R"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 13" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 13" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="10 13" id="KW-0675">Receptor</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 13" id="KW-0807">Transducer</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="50"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="88"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
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            <begin position="133"/>
            <end position="155"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="184"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="240"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="14">
        <location>
            <begin position="27"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003878"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022507"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023040"/>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU364061"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="318" mass="36348" checksum="78953EC0DE3C0B18" modified="2023-09-13" version="1">FVTTPARFKSLFLGIIFSLQTTFGILGNFSLLYHYLFLYLTGCRLRSTDLIIKNVIAANLLVLFSMGIPHALSSFGWYQIFDYFGCKTFYYVCGVGRGMSISTTCLLSVFQAIMISPNNSRWAELKVKAPRCIVPSILLCWILQVLVNVVFPMFMTSNWSNKNITNQKDFGYCSAVQHDKIRDFLYAALLSSPDVFCLGLMLWSSSSMVFILYRHKQRVQHLRRTNVSSRSSPESRATKTILLLVSTFVYFYTLSSIFRVCLALFNSPNWFLLNTNSGITLCFPTLSPFLFMSCDSSVSKFCFTWIRNTKSPNIIRNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K3Y2</accession>
    <name>A0A9L0K3Y2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Zinc finger protein 620</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ZNF620</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>2-hydroxy-17beta-estradiol + S-adenosyl-L-methionine = 2-hydroxy-3-methoxy-17beta-estradiol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28744"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:89268"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:53093"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>2-hydroxy-17beta-estradiol + S-adenosyl-L-methionine = 2-methoxy-17beta-estradiol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53088"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28744"/>
            <dbReference type="ChEBI" id="CHEBI:28955"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:53089"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>2-hydroxyestrone + S-adenosyl-L-methionine = 2-hydroxy-3-methoxy-estrone + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53108"/>
            <dbReference type="ChEBI" id="CHEBI:1156"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:136980"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:53109"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>2-hydroxyestrone + S-adenosyl-L-methionine = 2-methoxyestrone + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53100"/>
            <dbReference type="ChEBI" id="CHEBI:1156"/>
            <dbReference type="ChEBI" id="CHEBI:1189"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:53101"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>4-hydroxy-17beta-estradiol + S-adenosyl-L-methionine = 4-methoxy-17beta-estradiol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53096"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:62845"/>
            <dbReference type="ChEBI" id="CHEBI:136975"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:53097"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>4-hydroxyestrone + S-adenosyl-L-methionine = 4-methoxyestrone + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53104"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:87602"/>
            <dbReference type="ChEBI" id="CHEBI:136972"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:53105"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a catechol + S-adenosyl-L-methionine = a guaiacol + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17877"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33566"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:134251"/>
            <dbReference type="EC" id="2.1.1.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:17878"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
            <orientation evidence="2">Extracellular side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.6" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0K4I9"/>
    <dbReference type="Ensembl" id="ENSEAST00005074431.1">
        <property type="protein sequence ID" value="ENSEASP00005057267.1"/>
        <property type="gene ID" value="ENSEASG00005035506.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000155317"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016206">
        <property type="term" value="F:catechol O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042424">
        <property type="term" value="P:catecholamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032502">
        <property type="term" value="P:developmental process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042417">
        <property type="term" value="P:dopamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000054">
        <property type="entry name" value="Catechol O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002935">
        <property type="entry name" value="SAM_O-MeTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43836:SF3">
        <property type="entry name" value="CATECHOL O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43836">
        <property type="entry name" value="CATECHOL O-METHYLTRANSFERASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01596">
        <property type="entry name" value="Methyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51682">
        <property type="entry name" value="SAM_OMT_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0128">Catecholamine metabolism</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0531">Neurotransmitter degradation</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00049079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005057267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="374" mass="40649" checksum="3634A3E4D331A23E" modified="2023-09-13" version="1">MVPGNLGSGATTNGRTQLRLGCTGASTGHSTQQHTPGAPRATSPPRPPYLPRPAPHPPRPGSATGSARRVPAGPPPSPPSRASPRGDLRRRAARRAACTGTAPGEMLEPTPMLLAAFSLGLALLPLLFFLRRWGWLLIGWNEFILQPIHNLLMGDSKEQRILRHVLQHAVAGDPQSVLETIDAYCSQKEWAMNVGDKKGQFLDAVVQEQQPSVLLELGAYCGYSAVRMARLLPPGARLLTIEINPDYAAITQQMLDFAGLQDRVTVVLGASQDIIPQLKKKYDVDTLDVVFLDHWKDRYLPDTLLLEECGLLRKGTVLLADNVIVPGAPDFLAHVRGSGRFECTHFSSYLEYSLWVVDGVEKAVYLGPGSPAQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K675</accession>
    <name>A0A9L0K675_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Interleukin 13 receptor subunit alpha 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">IL13RA2</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K675"/>
    <dbReference type="Ensembl" id="ENSEAST00005052123.1">
        <property type="protein sequence ID" value="ENSEASP00005060966.1"/>
        <property type="gene ID" value="ENSEASG00005020346.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000159971"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009897">
        <property type="term" value="C:external side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004896">
        <property type="term" value="F:cytokine receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016064">
        <property type="term" value="P:immunoglobulin mediated immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035772">
        <property type="term" value="P:interleukin-13-mediated signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002638">
        <property type="term" value="P:negative regulation of immunoglobulin production"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:negative regulation of mast cell degranulation"/>
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            <begin position="1"/>
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</entry>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
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                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Protein modification; protein ubiquitination.</text>
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    <comment type="subunit">
        <text evidence="7">Stably associated with the tyrosine-phosphorylated IL3 receptor beta chain and tyrosine-phosphorylated EPO receptor (EPOR).</text>
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        <property type="term" value="P:intracellular signal transduction"/>
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        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Cytokine-inducible SH2-containing protein b"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Suppressor of cytokine signaling 2"/>
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        <property type="entry name" value="PHOSPHATIDYLINOSITOL 3-KINASE REGULATORY SUBUNIT"/>
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    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
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        <location>
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            <begin position="242"/>
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            <begin position="205"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="9">
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            <begin position="7"/>
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    <evidence type="ECO:0000256" key="1">
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    </evidence>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K6S3</accession>
    <name>A0A9L0K6S3_EQUAS</name>
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        <recommendedName>
            <fullName evidence="4 9">Beta-casein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">CSN2</name>
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    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 9">Important role in determination of the surface properties of the casein micelles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the beta-casein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K6S3"/>
    <dbReference type="Ensembl" id="ENSEAST00005071701.1">
        <property type="protein sequence ID" value="ENSEASP00005058062.1"/>
        <property type="gene ID" value="ENSEASG00005016726.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000001890"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004869">
        <property type="term" value="F:cysteine-type endopeptidase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007595">
        <property type="term" value="P:lactation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001588">
        <property type="entry name" value="Casein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016345">
        <property type="entry name" value="Casein_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031305">
        <property type="entry name" value="Casein_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11500">
        <property type="entry name" value="BETA CASEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11500:SF0">
        <property type="entry name" value="BETA-CASEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00363">
        <property type="entry name" value="Casein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002372">
        <property type="entry name" value="Beta-casein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00306">
        <property type="entry name" value="CASEIN_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0494">Milk protein</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040389241" description="Beta-casein" evidence="11">
        <location>
            <begin position="16"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR002372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005058062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="241" mass="27092" checksum="A82651CB9237BF86" modified="2023-09-13" version="1">MKILILACLVALALAREKEELNVSSETVESLSSNEPDSSSEESITHINKEKVQKFKHEGQQQREVEHQDKISRFVQPQPVVYPYAEPVPYAVVPQNILPLAQPPIVPFLQPEIMEVSQAKETLLPKRKVMPFLKSPIVPFSERQILNPTNGENLRLPVHLIQPFMHQVPQSLLQTLMLPSQPVLSPPQSKVAPFPQPVVPYPQRDTPVQAFLLYQDPQLGLTGEFDPATQPIVPVHNPVIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K798</accession>
    <name>A0A9L0K798_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">p-selectin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">CD62 antigen-like family member P</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="20">Granule membrane protein 140</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">Leukocyte-endothelial cell adhesion molecule 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Platelet activation dependent granule-external membrane protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">SELP</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="22">Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG. Mediates cell-cell interactions and cell adhesion via the interaction with integrin alpha-IIb/beta3 (ITGA2B:ITGB3) and integrin alpha-V/beta-3 (ITGAV:ITGB3).</text>
    </comment>
    <comment type="subunit">
        <text evidence="23">Interacts with SNX17. Interacts with SELPLG/PSGL1 and PODXL2 and mediates neutrophil adhesion and leukocyte rolling. This interaction requires the sialyl-Lewis X epitope of SELPLG and PODXL2, and specific tyrosine sulfation on SELPLG. Interacts (via C-type lectin domain) with alpha-IIb/beta3 integrin ITGA2B:ITGB3 and alpha-V/beta-3 integrin ITGAV:ITGB3. Interacts with alpha5/beta1 integrin ITGA5:ITGB1 and alpha4/beta1 integrin ITGA4:ITGB.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the selectin/LECAM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="24">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K798"/>
    <dbReference type="Ensembl" id="ENSEAST00005069648.1">
        <property type="protein sequence ID" value="ENSEASP00005061336.1"/>
        <property type="gene ID" value="ENSEASG00005019024.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000161063"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00033">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="CDD" id="cd03592">
        <property type="entry name" value="CLECT_selectins_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00054">
        <property type="entry name" value="EGF_CA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.100.10:FF:000007">
        <property type="entry name" value="L-selectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.25.10:FF:000176">
        <property type="entry name" value="Selectin P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.70.10:FF:000001">
        <property type="entry name" value="Selectin P"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.70.10">
        <property type="entry name" value="Complement Module, domain 1"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050350">
        <property type="entry name" value="Compl-Cell_Adhes-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000742">
        <property type="entry name" value="EGF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033991">
        <property type="entry name" value="Selectin_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002396">
        <property type="entry name" value="Selectin_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035976">
        <property type="entry name" value="Sushi/SCR/CCP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000436">
        <property type="entry name" value="Sushi_SCR_CCP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325">
        <property type="entry name" value="COMPLEMENT COMPONENT-RELATED SUSHI DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19325:SF484">
        <property type="entry name" value="P-SELECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00008">
        <property type="entry name" value="EGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00084">
        <property type="entry name" value="Sushi"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00343">
        <property type="entry name" value="SELECTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00032">
        <property type="entry name" value="CCP"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57535">
        <property type="entry name" value="Complement control module/SCR domain"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57196">
        <property type="entry name" value="EGF/Laminin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00022">
        <property type="entry name" value="EGF_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01186">
        <property type="entry name" value="EGF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50026">
        <property type="entry name" value="EGF_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50923">
        <property type="entry name" value="SUSHI"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0106">Calcium</keyword>
    <keyword evidence="12" id="KW-0130">Cell adhesion</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15 24" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4 24" id="KW-0245">EGF-like domain</keyword>
    <keyword evidence="16" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0430">Lectin</keyword>
    <keyword evidence="14 26" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="5 25" id="KW-0768">Sushi</keyword>
    <keyword evidence="6 26" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 26" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="26">
        <location>
            <begin position="708"/>
            <end position="732"/>
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    <feature type="domain" description="EGF-like" evidence="27">
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</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K7B6</accession>
    <name>A0A9L0K7B6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Carnitine O-palmitoyltransferase 2, mitochondrial</fullName>
            <ecNumber evidence="4">2.3.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Carnitine palmitoyltransferase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="32" type="primary">CPT2</name>
    </gene>
    <organism evidence="32 33">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="32 33" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="32" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="32" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Involved in the intramitochondrial synthesis of acylcarnitines from accumulated acyl-CoA metabolites. Reconverts acylcarnitines back into the respective acyl-CoA esters that can then undergo beta-oxidation, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Active with medium (C8-C12) and long-chain (C14-C18) acyl-CoA esters.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>(5Z)-tetradecenoyl-CoA + (R)-carnitine = O-(5Z)-tetradecenoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:44852"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:84649"/>
            <dbReference type="ChEBI" id="CHEBI:84650"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>(9Z)-tetradecenoyl-CoA + (R)-carnitine = O-(9Z)-tetradecenoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:44848"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:65060"/>
            <dbReference type="ChEBI" id="CHEBI:84647"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>(R)-carnitine + (9Z)-octadecenoyl-CoA = O-(9Z)-octadecenoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:44856"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:84651"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>(R)-carnitine + hexadecanoyl-CoA = O-hexadecanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:12661"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:17490"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57379"/>
            <dbReference type="EC" id="2.3.1.21"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="28">
            <dbReference type="Rhea" id="RHEA:12663"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="27">
            <text>(R)-carnitine + octadecanoyl-CoA = O-octadecanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:44840"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="ChEBI" id="CHEBI:84644"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="26">
            <text>4,8-dimethylnonanoyl-CoA + (R)-carnitine = O-4,8-dimethylnonanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:44860"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:77061"/>
            <dbReference type="ChEBI" id="CHEBI:84654"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>decanoyl-CoA + (R)-carnitine = O-decanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:44828"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:28717"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:61430"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>dodecanoyl-CoA + (R)-carnitine = O-dodecanoyl-R-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:40279"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57375"/>
            <dbReference type="ChEBI" id="CHEBI:77086"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>eicosanoyl-CoA + (R)-carnitine = O-eicosanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:44844"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57380"/>
            <dbReference type="ChEBI" id="CHEBI:84645"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>octanoyl-CoA + (R)-carnitine = O-octanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:17177"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:18102"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57386"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="25">
            <text>tetradecanoyl-CoA + (R)-carnitine = O-tetradecanoyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:44832"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:84634"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 30">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.21" evidence="4"/>
    <dbReference type="RefSeq" id="XP_044626746.1">
        <property type="nucleotide sequence ID" value="XM_044770811.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K7B6"/>
    <dbReference type="Ensembl" id="ENSEAST00005043562.1">
        <property type="protein sequence ID" value="ENSEASP00005061351.1"/>
        <property type="gene ID" value="ENSEASG00005014524.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106833186"/>
    <dbReference type="KEGG" id="eai:106833186"/>
    <dbReference type="CTD" id="1376"/>
    <dbReference type="GeneTree" id="ENSGT01150000286999"/>
    <dbReference type="OMA" id="HILVMRR"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004095">
        <property type="term" value="F:carnitine O-palmitoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006853">
        <property type="term" value="P:carnitine shuttle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001701">
        <property type="term" value="P:in utero embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001676">
        <property type="term" value="P:long-chain fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120162">
        <property type="term" value="P:positive regulation of cold-induced thermogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.275.20:FF:000001">
        <property type="entry name" value="carnitine O-palmitoyltransferase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1280.180:FF:000001">
        <property type="entry name" value="Carnitine O-palmitoyltransferase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.10:FF:000010">
        <property type="entry name" value="carnitine O-palmitoyltransferase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.20">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042572">
        <property type="entry name" value="Carn_acyl_trans_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF51">
        <property type="entry name" value="CARNITINE O-PALMITOYLTRANSFERASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00439">
        <property type="entry name" value="ACYLTRANSF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00440">
        <property type="entry name" value="ACYLTRANSF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0007">Acetylation</keyword>
    <keyword evidence="14 30" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="13" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="33" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 30" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="31">
        <location>
            <begin position="52"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="29">
        <location>
            <position position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005005"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
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    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040346"/>
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            <dbReference type="ARBA" id="ARBA00042919"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00046213"/>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047431"/>
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    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00047449"/>
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    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00047809"/>
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    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00047819"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048565"/>
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    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048758"/>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00048764"/>
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    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00048991"/>
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    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00048999"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049066"/>
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    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00049150"/>
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    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="RuleBase" id="RU003801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="31">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="32">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005061351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="33">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="658" mass="73888" checksum="BA75689339837946" modified="2023-09-13" version="1">MVTRLLLRPWARGPAMGPAAPCRPLSAGSRPGQYLQRSLVPTMHYQDSLPRLPIPKLEDTIRRYLNAQKPLLDDSQFRKTEQLCKSFESGIGKELHEQLVAQDKQNKHTSYISGPWFDMYLTARDSIVLNFNPFIAFNPDPKSEYNDQLTRATNLTVSAVRFLKTLRAGLLEPEVFHLNPAKSDTDTFKRLIRFVPSSLSWYGAYLVNAYPLDMSQYFRLFNSTRLPRPSRDELFTDDKARHLLVLRKGHFYVFDVLDQDGNIVSPSEIQAHLKYILSDNSPAPEFPLAYLTSENRDVWAELRQKLVNGGNEETLRKVDSAVFCLCLDDFPIKDLIHLSHSMLHGDGTNRWFDKSFNLVIAEDGTAGVHFEHAWGDGVAVLRFFHEVFKDSTQAPAITPQSQPARTDSSIAVQKLSFKLSDALKTGISAAKDKFDATVTTLTIDIIRFQRGGKEFLKKQKLSPDSVAQLAFQMAFLRQYGQTVATYESCSTAAFKHGRTETIRPASVFTKRCSEAFVREPSKHSAAELQQMMAECSKYHSRLTKEAAMGQGFDRHLFVLRYLAAAKGISVPELFLDPAYGQMNHNVLSTSTLSSPEVSLGGFAPVVPDGFGVGYAVHDDWIGCNVSSYPGRNSQEFLQCVEKALEDIFDALEGKAIKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KC47</accession>
    <name>A0A9L0KC47_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">GABA type A receptor associated protein like 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">GABARAPL1</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the ATG8 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014706407.1">
        <property type="nucleotide sequence ID" value="XM_014850921.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0KC47"/>
    <dbReference type="SMR" id="A0A9L0KC47"/>
    <dbReference type="Ensembl" id="ENSEAST00005042645.1">
        <property type="protein sequence ID" value="ENSEASP00005062388.1"/>
        <property type="gene ID" value="ENSEASG00005034033.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106837407"/>
    <dbReference type="KEGG" id="eai:106837407"/>
    <dbReference type="CTD" id="23710"/>
    <dbReference type="GeneTree" id="ENSGT00940000156876"/>
    <dbReference type="OMA" id="KNQIRAK"/>
    <dbReference type="OrthoDB" id="6738456at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036064">
        <property type="term" value="C:ciliary basal body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030957">
        <property type="term" value="F:Tat protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061723">
        <property type="term" value="P:glycophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd16127">
        <property type="entry name" value="Ubl_ATG8_GABARAP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000037">
        <property type="entry name" value="Gamma-aminobutyric acid receptor-associated protein-like 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004241">
        <property type="entry name" value="Atg8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10969">
        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEINS 1A/1B LIGHT CHAIN 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02991">
        <property type="entry name" value="ATG8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8 10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="lipid moiety-binding region" description="Phosphatidylserine amidated glycine; alternate" evidence="10">
        <location>
            <position position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR604241-50"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004384"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005062388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="117" mass="14044" checksum="7F56E345255F564B" modified="2023-09-13" version="1">MKFQYKEDHPFEYRKKEGEKIRKKYPDRVPVIVEKAPKARVPDLDKRKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNTIPPTSATMGQLYEDNHEEDYFLYVAYSDESVYGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KLT5</accession>
    <name>A0A9L0KLT5_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Hes related family bHLH transcription factor with YRPW motif 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">HEY2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the HEY family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014713799.1">
        <property type="nucleotide sequence ID" value="XM_014858313.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0KLT5"/>
    <dbReference type="Ensembl" id="ENSEAST00005042798.1">
        <property type="protein sequence ID" value="ENSEASP00005062891.1"/>
        <property type="gene ID" value="ENSEASG00005024401.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106842000"/>
    <dbReference type="KEGG" id="eai:106842000"/>
    <dbReference type="CTD" id="23493"/>
    <dbReference type="GeneTree" id="ENSGT00940000160636"/>
    <dbReference type="OMA" id="DTTPCRL"/>
    <dbReference type="OrthoDB" id="6371181at2759"/>
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        <property type="component" value="Chromosome 24"/>
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        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0017053">
        <property type="term" value="C:transcription repressor complex"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0000987">
        <property type="term" value="F:cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061629">
        <property type="term" value="F:RNA polymerase II-specific DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009948">
        <property type="term" value="P:anterior/posterior axis specification"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003180">
        <property type="term" value="P:aortic valve morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060842">
        <property type="term" value="P:arterial endothelial cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035910">
        <property type="term" value="P:ascending aorta morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060413">
        <property type="term" value="P:atrial septum morphogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003214">
        <property type="term" value="P:cardiac left ventricle morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010659">
        <property type="term" value="P:cardiac muscle cell apoptotic process"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060038">
        <property type="term" value="P:cardiac muscle cell proliferation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014898">
        <property type="term" value="P:cardiac muscle hypertrophy in response to stress"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003215">
        <property type="term" value="P:cardiac right ventricle morphogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060948">
        <property type="term" value="P:cardiac vascular smooth muscle cell development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045165">
        <property type="term" value="P:cell fate commitment"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090102">
        <property type="term" value="P:cochlea development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060977">
        <property type="term" value="P:coronary vasculature morphogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035912">
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:endocardial cushion to mesenchymal transition involved in heart valve formation"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060347">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060716">
        <property type="term" value="P:labyrinthine layer blood vessel development"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0014031">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003150">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070168">
        <property type="term" value="P:negative regulation of biomineral tissue development"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0010667">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010629">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045746">
        <property type="term" value="P:negative regulation of Notch signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051151">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060633">
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    <dbReference type="GO" id="GO:0007219">
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003151">
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    <dbReference type="GO" id="GO:0060045">
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    <dbReference type="GO" id="GO:0003184">
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    <dbReference type="GO" id="GO:0045607">
        <property type="term" value="P:regulation of inner ear auditory receptor cell differentiation"/>
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    <dbReference type="GO" id="GO:0003195">
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        <property type="term" value="P:umbilical cord morphogenesis"/>
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    <dbReference type="GO" id="GO:0001570">
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    <dbReference type="GO" id="GO:0055015">
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    <dbReference type="GO" id="GO:0003222">
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    </dbReference>
    <dbReference type="CDD" id="cd18920">
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        <property type="match status" value="1"/>
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        <property type="entry name" value="hairy/enhancer-of-split related with YRPW motif protein 1"/>
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        <property type="entry name" value="Orange_dom"/>
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        <property type="entry name" value="BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED"/>
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    <dbReference type="Pfam" id="PF07527">
        <property type="entry name" value="Hairy_orange"/>
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    <dbReference type="Pfam" id="PF00010">
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    <dbReference type="SMART" id="SM00511">
        <property type="entry name" value="ORANGE"/>
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    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
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    <dbReference type="PROSITE" id="PS51054">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0914">Notch signaling pathway</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="48"/>
            <end position="103"/>
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    <feature type="domain" description="Orange" evidence="12">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
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    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="307"/>
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    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="8"/>
            <end position="18"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="22"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="312"/>
            <end position="325"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
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    <evidence type="ECO:0000256" key="2">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1699"/>
            <end position="1714"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001465.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="2032" mass="231404" checksum="1C904AE53747AA18" modified="2023-09-13" version="2">MPKSGFTKPVQSENSDSDSNMVEKPYGRKSKDKIASYSKTPKIDRSDVGKEMKEKSSMKRKLPFTISPSRNEERDSDTEKEGPEKKKTKKEAGNKKSTPVSILFGYPLSERKQMALLMQMTARDNSPDSTPNHPSQTTPAQKKTPSSSSRQKDKVNKRNERGETPLHMAAIRGDVKQVKELISLGANVNVKDFAGWTPLHEACNVGYYDVAKILIAAGADVNTQGLDDDTPLHDSASSGHRDIVKLLLRHGGNPFQANKHGERPVDVAETEELELLLKREVPLSDDDESYTDSEEARSVNPSSVDENIDSETEKDSLICESKQIIPSKAPLPSALDEYEFKDDDDEELNKMIDDRHILRKELRKESESEIEKNSLFAKQEKTFYPKSLKSKKQKPSRVLYSSSESSDEEILQNKKVSAPCSVPETSNSDTQTKKEYGISNEHKQKGKVKRKLKNQNKNKENQELKQEKEGKENTRVTNLAVNTTLDCSEKTREEGNFRKSFSPKDDSSLHLFHISTGKSPKHSCGLSEKQSSPLKQEHTKTCLSPGSSETSLQPDLVRYDNTESEFLPESSSVKSCKHKEKSKHQKDFHLEFGEKSNAKIKDEDHSPTFENSDCTLKKMDKEGKTLKKHKLKHKEREKEKHKKEIEGEKEKYKNRDGTKELQRSVEFDREFWKENFFKSDETEDLFLNMEHESLILEKKSKLEKNIKDDKSTKEKHVSKERNFKEEREKIKKESEKSFREEKIKDLKEERENVPIDKETEFSSLGVSVSEESIGLHSMEKEIEIEKHIKESKEKSEKRFQAKEKDIEKTEKKNSEKEKKKHEHKSEKDKLDLSECVDKIKEKDKLYSHHTEKCHKEGEKIKNITAIKKADDREKSREKIERKHDKEKSDRERHVAESKEKHLMEKKPKQLDNSDYTKSEKSKNKDREIDKKEKSREKESVNITNSKHFQEEKKSSIADSSKAQHEKTLSLKEKTKDEPLKTPDGKEKDKKDKDIDRYKERDKHKDKIQLNSLLKLKSEADKPKPKSSPASKDARPKEKRLVNDDLMQTSFERMLSLKDLEIEQWHKKHKEKIKQKEKERLRNRNCLELKVKDKEKTKHALVESKNKELIRSKSSELTDAYTKEKQSKDAISNRSQSVDTKNIMNLGKSSFVSDNSLNRSPRSESEKLGLSSRSVSLISVASSEDSCHTTVTTPRPPVEYDSDFMLEGSDSQMSFSQSPFFPVAKSPALHERELDSLAELPERIKPPYTSRLPTSHLRSSSIEDAKLVISEGRPTVEVRRCSMPSVICEHTKQFQTISEESNQSGLAVPRDICPSPKPEVSLHVPERELSNVSNIHSSFAASPTRSVNSKYISADINVTKSTAAVSTLMNSPVHLEPSNQVGVIQNKSWGIPVDRLESLSTSDFISPNSSTPDQDSSVQGFCNSENKILKEQNTDFLSLHQTDPPGHSCAQDPPSFLPSQHPCSFHSQSLSDAESVSKHVSLSSVANQEPGILQQKNAVQIISSALDSESAKDMENTFVLTDVQKTDAFVPVYSESIVQEASPNFEKANTLPVLPSEKDFSGSDASSQLSTPYAFSRLMYKSSGSHGVENSTSDIQIISHEKESKLESLVLTHLNDKCDSDLCDMNAGMPKGNLNEQDNPKHCPESEKCLLSIEDEESQQSTLSSLENQSQQSAQPEVHKYGQLVKVELEENAEDDKTENQIPQRMTRNKANTVASQNKQILASCALLPEKDSESSSPRGRIRLTEEDDPQIHHPRKRKVSRVPQPVQVSPSLLQAKEKTQQSLAAIVDSLKLDEIQPYSSERANPYFEYLHIRKKIEEKRKLLCSVIPQAPQYYDEYVTFNGSYLLDGNPLSKICIPTITPPPSLSDPLKELFRQQEVVRMKLRLQHSIEREKLIVSNEQEVLRVHYRAARTLANQTLPFSACTVLLDAEVYSVPLDAQSDDSKTSVRDRFNARQFMSWLQDVDDKFDKLKTCLLMRQQHEAAALNAVQRLEWQLKLQELDPATYKSISIYEIQEFYVPLVDVNDDFELTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KUL8</accession>
    <name>A0A8C4KUL8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Zinc finger CCCH-type containing 18</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ZC3H18</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="RefSeq" id="XP_044616974.1">
        <property type="nucleotide sequence ID" value="XM_044761039.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KUL8"/>
    <dbReference type="Ensembl" id="ENSEAST00005000725.2">
        <property type="protein sequence ID" value="ENSEASP00005000611.2"/>
        <property type="gene ID" value="ENSEASG00005000446.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106828261"/>
    <dbReference type="KEGG" id="eai:106828261"/>
    <dbReference type="CTD" id="124245"/>
    <dbReference type="GeneTree" id="ENSGT00730000111190"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 28"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052647">
        <property type="entry name" value="Zinc_finger_CCCH-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041367">
        <property type="entry name" value="Znf-CCCH_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46582">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46582:SF1">
        <property type="entry name" value="ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18044">
        <property type="entry name" value="zf-CCCH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="E3 ligase CCCH-type zinc finger" evidence="5">
        <location>
            <begin position="246"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="409"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="61"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="168"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="185"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="414"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="528"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="563"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="658"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="679"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="708"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="752"/>
            <end position="766"/>
        </location>
    </feature>
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        <location>
            <begin position="776"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="794"/>
            <end position="814"/>
        </location>
    </feature>
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        <location>
            <begin position="840"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="909"/>
            <end position="943"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF18044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000611.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="972" mass="108467" checksum="62294C50BFE158FA" modified="2023-09-13" version="2">MDVAESPERDPRSPEDEEEEQQGLSDDDILRESGSDQDLDGAGGRASDLEDEENPAPGLSQEEEESRSDEEDQDQDSEAQELSRGPASPPRVEEGDGGEEDRTSDLRDEASSVTRELDEHELDYDEEVPEEPAPAGQEDEAERAGPEGDEEKGAGALGEEGPAGAHGVQDREPLEAAKEKRKEDDDGEIDDGEIDDDDLEEGEVKDPSDRKVRPRPTCRFFMKDVVTPLSTLLPPISNSIPFRGQVKGNCTWGMNCRFIHPGVNDKGNYSLITKAEPFPPNGAPPLGPHPLMPANPWGGPIVDEILPPPPPEPPTESAWERGLRHAKEVLKKATIRKEQEPDFEEKRFTVTIGEDEREFDKENEAFRDWNYRITRDVRDTALEPYADPYYDYEIERFWRGGQYENFRVQYTETEPYHNYRERERERERENRQRERERERDRERERERRQRERERERERERDKERQRRKEEWERERAKRDEKDRQHRDRDRDKEREKEKEKPKPRSPQPPSRQAEPPKKEVASAGPQVKRADEWKDPWRRSKSPKKKLGVSVSPSRARRRRKTSASSASASNSSRSSSRSSSYSGSGSSRSRSRSSSYSSYSSRSSRHSSFSGSRSRSRSFSSSPSPSPTPSPHRPSIRTKGEPAPPPGKAGEKLVKKPAPPPAPPQAPKTTAPAPEPAKPGDPREARRRERQARTPPRRRTISGSGSGSSYSGSSSRSRSLSVSSVSSVSSATSSSSSVHSVDSDDMYADLASPVSSASSRSPTPAQTKKEKGKSKKEDGVKEEKRKRDSSTQPPKSSKPSAGGKSSQQPTTPQQAPPGQPQQGAFMAHKEIKLTLLNKAADKASRKRYEPSDKDRQSPPPAKRANVSPDRGSRDRKSGGRLSSPKPERQRGQNSKALVAPPDRKRQLSPQSKSSSKVTSVPGKASDAGATTATGTKSGKASTLSRREELLKQLKAVEDAIARKRAKIPGKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KWR8</accession>
    <name>A0A8C4KWR8_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">SRC kinase signaling inhibitor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SRCIN1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
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                <person name="Li"/>
                <person name="H."/>
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                <person name="Huang"/>
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                <person name="Sun"/>
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                <person name="Wang"/>
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                <person name="Fang"/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Gao"/>
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                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
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                <person name="Liu"/>
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                <person name="Liu"/>
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        <property type="entry name" value="Collagen_superfamily"/>
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        <property type="entry name" value="COLLAGEN ALPHA"/>
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        <property type="entry name" value="COLLAGEN TRIPLE HELIX REPEAT"/>
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        <property type="match status" value="5"/>
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    <sequence length="688" mass="64752" checksum="087437E5B22B02D5" modified="2022-01-19" version="1">MAAAAAPRSLLVLLQVLGLALAQIRGPPGEQGPPGPPGPPGVPGSDGIDGDKGPPGKAGPPGPKGEPGQAGPDGPDGKPGTDGLTGAKGEPGPMGIPGVKGQPGLPGPPGLPGPGFAGPPGPPGPVGLPGEIGITGPKGDPGPDGPSGPPGPPGKPGRPGTIAGLEGSADFLCPTNCPAGAKGPPGLQGVKGHPGKRGALGDSGRQGKPGPKGDVGASGEQGIPGPPGPQGVRGYPGMEGPKGETGPRGYKGMLGSIGAAGSPGEEGPRGPPGRAGEKGDVGSQGVRGPQGITGPKGATGPPGIDGKDGTPGTPGVKGSAGQAGRPGNPGHQGLAGVPGQPGTKGGPGDKGEPGQQGLPGFSGPPGKEGEPGPRGEIGPRGIVGQKGDQGERGPVGQPGPQGRQGPKGEQGPPGIPGPQGLPGIKGDKGSPGKTGPRGGAGDPGVAGLPGEKGEKGESGEPGPKGQQGVRGEPGYPGPSGDAGAPGVQGYPGPPGPRGLAGDRGVPGQPGRQGVAGRDASDQHIVDVVLKMLQEQLAEVAVSAKREALGATGMVGPPGPPGPPGYPGKQGPHGHPGPRGIPGIVGAMGQIGNTGPKGKRGEKGDQGDTGRGHPGMPGPPGIPGLPGRPGQAINGKDGDRGSPGAPGEAGRPGLPGPVGLPGFCEPAACLGASAYASARLTEPGSIKGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KZ81</accession>
    <name>A0A8C4KZ81_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">GTPase RhebL1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Ras homolog enriched in brain-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">RHEBL1</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Binds GTP and exhibits intrinsic GTPase activity. May activate NF-kappa-B-mediated gene transcription. Promotes signal transduction through MTOR, activates RPS6KB1, and is a downstream target of the small GTPase-activating proteins TSC1 and TSC2.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="16">Interacts with MTOR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13">Endomembrane system</location>
            <topology evidence="13">Lipid-anchor</topology>
            <orientation evidence="13">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the small GTPase superfamily. Rheb family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014705614.1">
        <property type="nucleotide sequence ID" value="XM_014850128.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KZ81"/>
    <dbReference type="Ensembl" id="ENSEAST00005001423.2">
        <property type="protein sequence ID" value="ENSEASP00005001257.2"/>
        <property type="gene ID" value="ENSEASG00005001042.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106836900"/>
    <dbReference type="KEGG" id="eai:106836900"/>
    <dbReference type="CTD" id="121268"/>
    <dbReference type="GeneTree" id="ENSGT00940000161665"/>
    <dbReference type="OrthoDB" id="25818at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000971">
        <property type="entry name" value="GTPase RhebL1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0636">Prenylation</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00056542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00065080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00076144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001257.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="200" mass="22247" checksum="04FC17B616C33872" modified="2023-09-13" version="2">MEAALERGCRARMEDGGGKAGVVVVCGELQEAGAWKTSLAHQFVEGEFLEGYDPTVENTYSKIVTLGKDEFHLHLVDTAGQDEYSILPYSFIIGVHGYVLVYSVTSLHSFQVIESLYQKLHEGHGKTRLPVVLVGNKADLSPYREVQADEGKKLAESWGATFMESSARENQLTQGIFTKVIQEIARVENSYGQERRCQLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KZN2</accession>
    <name>A0A8C4KZN2_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transmembrane protein 247</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">TMEM247</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4KZN2"/>
    <dbReference type="Ensembl" id="ENSEAST00005004401.2">
        <property type="protein sequence ID" value="ENSEASP00005004012.2"/>
        <property type="gene ID" value="ENSEASG00005003049.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000002879"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 6"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029200">
        <property type="entry name" value="TMEM247"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36691">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36691:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 247"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15444">
        <property type="entry name" value="TMEM247"/>
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</entry>
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        <name type="scientific">Equus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
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                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001787.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="219" mass="22143" checksum="50DCDF2D50F4455D" modified="2023-09-13" version="2">MPAGEARAPLGQGLPPDVLAEQVELWWSQQPRRSALCFAVAVGLVAGCGAAGVALLSSTSSRSGEWRLAAGTVLCLLALLVLVKQLMSSAVQDMNCIRQPHHVALLRSGGAADALVVLLSGLVLLVTGLTLAGLASAPAPARPLAAMLSVGIALTASGSLLLLGLLLYQVGVSGHCPPIRSAPPATHSDHSGNGSIFSISGRLSAGQRHETTSSIASLI</sequence>
</entry>
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    <accession>A0A8C4L175</accession>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
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                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
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                <person name="A."/>
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                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Odorant receptor.</text>
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    <comment type="function">
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            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="7tmA_OR52E-like"/>
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        <property type="entry name" value="Olfactory receptor"/>
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    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450:SF170">
        <property type="entry name" value="OLFACTORY RECEPTOR 52E8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR26450">
        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 11" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 12" id="KW-0552">Olfaction</keyword>
    <keyword evidence="11" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="10 11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="29"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="67"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="243"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="276"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="13">
        <location>
            <begin position="47"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022725"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
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    <evidence type="ECO:0000256" key="8">
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    <evidence type="ECO:0000256" key="11">
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    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005002042.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="320" mass="36603" checksum="2EF5EF0A7D84316B" modified="2023-09-13" version="2">MTERMSLLNDTQFHPSSFLLLGIPSLEDVHVWIGFPFFFVYLVALLGNAAVLFVIQTEHSLHEPVYYFLAMLDSIDLGLSTATIPKMLGIFWFSFRDISFESCLSQMFFIHFFTAMESIVLVAMSFDRYIAICKPLQYTMILTNKIISIIAGIAILRSLCMVAPLVFLLLRLPFCEHHIIPHTYCEHMGIARLACASIKANIIFGLGDMSILLLDVFLITLSYVRILYTVFCLPSWEARFKALNTCGSHIGVILAFFTPAFFSFLTHRFGHSIPQYIHILLANLYVVVPPALNPVIYGIRTKQIRERVSWIFFRKIVNHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L439</accession>
    <name>A0A8C4L439_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Huntingtin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">HTT</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May play a role in microtubule-mediated transport or vesicle function.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Promotes the formation of autophagic vesicles.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with PFN1. Interacts through its N-terminus with PRPF40A. Interacts with PQBP1. Interacts with SETD2. Interacts with SH3GLB1. Interacts with SYVN. Interacts with TPR; the interaction is inhibited by forms of Huntingtin with expanded polyglutamine stretch. Interacts with ZDHHC13 (via ANK repeats). Interacts with ZDHHC17 (via ANK repeats). Interacts with F8A1/F8A2/F8A3. Found in a complex with F8A1/F8A2/F8A3, HTT and RAB5A; mediates the recruitment of HTT by RAB5A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
            <location evidence="3">Autophagosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the huntingtin family.</text>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005005965.2">
        <property type="protein sequence ID" value="ENSEASP00005005459.2"/>
        <property type="gene ID" value="ENSEASG00005003073.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015863"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 3"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005776">
        <property type="term" value="C:autophagosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030424">
        <property type="term" value="C:axon"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005814">
        <property type="term" value="C:centriole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030659">
        <property type="term" value="C:cytoplasmic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030425">
        <property type="term" value="C:dendrite"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016234">
        <property type="term" value="C:inclusion body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005770">
        <property type="term" value="C:late endosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099524">
        <property type="term" value="C:postsynaptic cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0099523">
        <property type="term" value="C:presynaptic cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048487">
        <property type="term" value="F:beta-tubulin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034452">
        <property type="term" value="F:dynactin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019900">
        <property type="term" value="F:kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002039">
        <property type="term" value="F:p53 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004721">
        <property type="term" value="F:phosphoprotein phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005522">
        <property type="term" value="F:profilin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044325">
        <property type="term" value="F:transmembrane transporter binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001237">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905337">
        <property type="term" value="P:positive regulation of aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905291">
        <property type="term" value="P:positive regulation of CAMKK-AMPK signaling cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045724">
        <property type="term" value="P:positive regulation of cilium assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904504">
        <property type="term" value="P:positive regulation of lipophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901526">
        <property type="term" value="P:positive regulation of mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031648">
        <property type="term" value="P:protein destabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042297">
        <property type="term" value="P:vocal learning"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000273">
        <property type="entry name" value="Huntingtin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000388">
        <property type="entry name" value="Huntingtin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048412">
        <property type="entry name" value="Htt_bridge"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048413">
        <property type="entry name" value="Htt_C-HEAT_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048411">
        <property type="entry name" value="Htt_N_HEAT_rpt-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000091">
        <property type="entry name" value="Huntingtin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028426">
        <property type="entry name" value="Huntingtin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024613">
        <property type="entry name" value="Huntingtin_N_HEAT_rpt-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10170:SF10">
        <property type="entry name" value="HUNTINGTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10170">
        <property type="entry name" value="HUNTINGTON DISEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20925">
        <property type="entry name" value="Htt_bridge"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20927">
        <property type="entry name" value="Htt_C-HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12372">
        <property type="entry name" value="Htt_N-HEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20926">
        <property type="entry name" value="Htt_N-HEAT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00375">
        <property type="entry name" value="HUNTINGTIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="11" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7" id="KW-0519">Myristate</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="514"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1162"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="2618"/>
            <end position="2646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="16">
        <location>
            <begin position="22"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="458"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="536"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1193"/>
            <end position="1208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="2618"/>
            <end position="2629"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="2630"/>
            <end position="2642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00065428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00068126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005459.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="3126" mass="345328" checksum="7C3C94762D2F7660" modified="2023-09-13" version="2">MATLEKLMKAFESLKSFQQQQQPPPPPPQPPQPPPQAQPPPPQQQQPPPPPPPPPGPAAAEEPLHRPKKELSATKKDRVNHCLTICENIVAQSLRNSPEFQKLLGIAMELFLLCSDDAESDVRMVADECLNKVIKALMDSNLPRLQLELYKEIKKNGAPRSLRAALWRFAELAHLVRPQKCRPYLVNLLPCLTRTSKRPEESVQETLAAAIPKIMASFGNFANDNEIKVLLKAFIANLKSSSPTIRRTAAGSAVSICQHSRRTQYFYTWLLNVLLGLLVPVEDEHSTLLILGVLLTLRYLVPLLQQQVKDTSLKGSFGVTRKEMEVSPSTEQLVQVYELTLHYTQHQDHNVVTGALELLQQLFRTPPPELLQALTTAGGLGRLAVAKDEPSCRSRSGSIVELIAGGGSSCSPVLSRKQKGKVLLGEAAALEEDSESRSDSSSPAFAASVKGEIGGELAASSGVSTPGSASSAADSAGHDIITEQPRSQHTLQPDSVDLTGCDLTSAATDGVEEDILSHSSSQISAVPSDPAMDLNDGTQASSPISDSSQTTTEGPDSAVTPSDSSEIVLDGPDGQYSGMQIGQPRDEGEEAAGLLPDEDVDAFTDSAVALQQAHLLERLGHSRQPSDSSIDKFVSRDEATEPGDQENKPCRIKGDIGRSADDDSAPLVHCVRLLSASFLLTGEKNALVPDRDVRVSVKALALSCVGAAVALHPESFFSKLYKVPLETTEYPEEQYVSDVLNYIDHGDPQVRGATAILCGTLVYSILSRSRFHVGDWVATVRTLTGNTFSLVDCIPLLQKTLKDESSVTCKLACTAVRHCVMSLCSSSYSEFGLQLITDVLTLRNSSYWLVRTELLETLAEIDFRLVSFLEAKAENIHRGAHHYTGRLKLQERVLNNVVIYLLGDEDPRVRHVAAASLMRLVPKLFYKCDQGQADPVVAVARDQSSVYLKLLMHETQPPSHFSVSTITRIYRGYNLLPSITDVTMENNLSRVIAAVSHELITSTTRALTFGCCEALCLLSTAFPVCVWSLGWHCGVPPLSASDEARKSCTVGMATMILTLLSSAWFPLDLSAHQDALILAGNLLAASAPKSLRSSWTSEEEANPAATKQEEIWPALGDRTLVPMVEQLFSHLLKVINICAHVLDDVAPGPAIKAALPSLTNPPSLSPIRRKGKEKEPGEQASVPLSPKKGNEASPASRQSDTSGPVPANKSSSLGSFYHLPSYLKLHDVLKATHANYKVTLDLQNSTEKFGGFLRSALDVLSQILELATLQDIGKCVEEILGYLKSCFSREPMMATVCVQQLLKTLFGTNLASQFDGLSSNPSKSQGRAQRLGSSSVRPGLYHYCFMAPYTHFTQALADASLRNMVQAEQDHDTSGWFDVLQKVSTQLKTNLTSVTKNRADKNAIHNHIRLFEPLVIKALKQYTTTTSVQLQKQVLDLLAQLVQLRVNYCLLDSDQVFIGFVLKQFEYIEVGQFRESEAIIPNIFFFLVLLSYERYHSKQIIGIPKIIQLCDGIMASGRKAVTHAIPALQPIVHDLFVLRGTNKADAGKELETQKEVVVSMLLRLIQYHQVLEMFILVLQQCHKENEDKWKRLSRQIADIILPMLAKQQMHIDSHEALGVLNTLFEILAPSSLRPVDMLLRSMFVTPDTMASVSTVQLWISGILAILRVLISQSTEDIVLSRIQELSFSPYLISCPIINRLRDGDSNSTPEEQGEGRQVKNLPEETFSRFLLQLVGILLEDIVTKQLKVEMSEQQHTFYCQELGTLLMCLIHIFKSGMFRRITAAASRLFTADGADSSFYGLESLNARVRSMVPTHPALVLLWCQILLLVSHTDYRWWAEVQQTPKRRSLSSTKSLCPQMSGEEEDSDLAAKLGMCNREIVRRGALILFCDYVCQNLHDSEHLTWLIVNHVQDLISLSHEPPVQDFISAVHRNSAASGLFIQAIQSRCENLSTPTTLKKTLQCLEGIHLSQSGAVLMLYVDKLLCTPLRVLARMVDTLACRRVEMLLAANLQSNMAQLPVEELNRIQEYLQNSGLAQRHQRLYSLLDRLRLATAPGSCGPSPPVTSHPLDGDGPLALETVNPDKDWYIRLVKSQCWTRSDSALLEGAELVNRLPPGDMSAFMMNSEFNLSLLAPCLGLGMREISGGQKSPLFEAARVVTLDRVTSMVQQLPPVHQVFQPFLPVEPSTYWGKVNDLFGDAVLYRSLTTLARALAQYLLVFSKLPSHLHLPPEKEKDTVKFVVMTLEALSWHLMHKQIPLSLDLQAGLDCCCLALQLPGLWSVLSSPEMVTHACSLIHCVRFLLEAIVVQPGDQLLSPERRTNTPKAAREDEVDSNTLNPKYITAACGMVAEMVESLPSVLALGHKRNSNTPAFLTSVLKNIIISLARLPLVNSYTRVPPLVWKLGWAPKPGGDFGTAFPEIPVEFLQEKEVFKEFIYRINALGWTSRTQFEETWATLLGVLVTQPLVMEQEESPPEEDTERTQINVLAVQAITSLVLSAMTVPVAGNPAVSCLEQQPRNKPLKALDTRFGRKLSIIRGIVEQEIQAMVSKRENIATHHLYQAWDPVPSLSPATTGALISHDKLLLQINPERELGNMSYKLGQVSIHSVWLGNSITPLREEEWDEEEEEEADVPAPSSAPTSPVNSRKHRAGVDIHSCSQFLLELYSRWILPSSSARRTPIILISEVVRSLLVVSDLFTERSQFEMMYLTLTELRRVHPSEDEILVQYLVPATCKAAAVLGMDKAVAEPVSRLLESTLRSSHLPSKIGALHGVLYVLECDLLDDTAKQLIPVISDYLLSNLKGVAHCVNIHSQQHVLVMCATAFYLIENYPLDVGPEFSASIIQMCGVMLSGSEESTPSIIYHCVLRGLERLLLSEQLSRLDAESLVKLSVDRVNVHSPHRAMAALGLMLTCMYTGKEKISPGRTSDPSPAAPDSESVIVAMERVSVLFDRIRKGFPCEARVVARILPQFLDDFFPPQDVMNKVIGEFLSNQQPYPQFMATVVYKVFQTLHSTGQSSMVRDWVMLSLSNFTQRTPVAMAVWSLSCFFVSASTSPWVSAVLPHVISRMGKLEQVDVNLFCLVATDFYRHQIEEELDRRAFQSVFEVVAAPGNPYHQLLTCLRNVHKVSTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L6G2</accession>
    <name>A0A8C4L6G2_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_070375405.1">
        <property type="nucleotide sequence ID" value="XM_070519304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L6G2"/>
    <dbReference type="Ensembl" id="ENSEAST00005003742.2">
        <property type="protein sequence ID" value="ENSEASP00005003413.2"/>
        <property type="gene ID" value="ENSEASG00005031031.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106831785"/>
    <dbReference type="GeneTree" id="ENSGT00940000154646"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd20679">
        <property type="entry name" value="CYP4F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.630.10:FF:000005">
        <property type="entry name" value="cytochrome P450 4F22 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050196">
        <property type="entry name" value="Cytochrome_P450_Monoox"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291:SF198">
        <property type="entry name" value="CYTOCHROME P450 4F3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24291">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 10 11" id="KW-0349">Heme</keyword>
    <keyword evidence="7 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 11" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="10">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="524" mass="59778" checksum="C6E06390D59EA8BC" modified="2023-09-13" version="2">MPQLSLSWLGLGPVAASLWLLLLLVGVSWLLARILVWTYTFYDIVRCLRCFPQPPKRNWFLGHLGLFPPTEEGLRDVTQLVANYPQGFVTWMGPVMPLVTLCHPDMVRTIVSASAAIAPKDVVFYRFLKPWLGDGLLLSGGDKWSRHRRMLTPALHFNILKSYVRIFNDSVNIMHAKWKRLASEGSAHLDMFEHISLMTLDSLQKCVFSFDSNCQEKPSEYISAVLELSALVTKRNQQITLHMDSLYHLIPDGWRFRRACRLVHDFTDSVIQERRRTLPDQGLDDLLKAKAKVKTLDFIDVLLLAKDEDGKELSDEDIRAEADTFMFAGHDTTASGLAWVLYNLARHPEHQERCRQEVRELLRDREPKEIEWDDLAQLPFLTMCIKESLRLHPPVTVISRRCTQDIVLPDGRVIPKGVICLISIFGTHHSPSVWPDPEVYDPFRFDPENTKETSPLAFIPFSAGPRNCIGQTFAMTEMKVVLALTLLSFRVLPAGEEPRRKPELILRAEGGLWLRVEPLSAGPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L856</accession>
    <name>A0A8C4L856_EQUAS</name>
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    <gene>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="8">Associates with the adapter-like complex 4 (AP-4) and may therefore play a role in vesicular trafficking of proteins at the trans-Golgi network.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with AP4B1 and AP4E1; the interaction is direct and mediates the association of TEPSIN with the adapter-like complex 4 (AP-4), a heterotetramer composed of AP4B1, AP4E1, AP4M1 and AP4S1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasmic vesicle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014719554.2">
        <property type="nucleotide sequence ID" value="XM_014864068.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L856"/>
    <dbReference type="Ensembl" id="ENSEAST00005008499.2">
        <property type="protein sequence ID" value="ENSEASP00005007790.1"/>
        <property type="gene ID" value="ENSEASG00005005682.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106845699"/>
    <dbReference type="KEGG" id="eai:106845699"/>
    <dbReference type="CTD" id="146705"/>
    <dbReference type="GeneTree" id="ENSGT00390000015076"/>
    <dbReference type="OMA" id="KASQRCP"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030662">
        <property type="term" value="C:coated vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="GO" id="GO:0005829">
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032588">
        <property type="term" value="C:trans-Golgi network membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03572">
        <property type="entry name" value="ENTH_like_Tepsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.90:FF:000029">
        <property type="entry name" value="AP-4 complex accessory subunit Tepsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013809">
        <property type="entry name" value="ENTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035802">
        <property type="entry name" value="ENTH/VHS_tepsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039273">
        <property type="entry name" value="TEPSIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058028">
        <property type="entry name" value="Tepsin_VHS/ENTH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21514">
        <property type="entry name" value="AP-4 COMPLEX ACCESSORY SUBUNIT TEPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21514:SF0">
        <property type="entry name" value="AP-4 COMPLEX ACCESSORY SUBUNIT TEPSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01417">
        <property type="entry name" value="ENTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25827">
        <property type="entry name" value="TVHS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50942">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="7" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="8"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="130"/>
            <end position="178"/>
        </location>
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            <begin position="196"/>
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        <location>
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            <end position="450"/>
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            <begin position="558"/>
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            <begin position="137"/>
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            <begin position="154"/>
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            <begin position="218"/>
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            <begin position="261"/>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
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        </source>
    </evidence>
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            <dbReference type="PROSITE" id="PS50942"/>
        </source>
    </evidence>
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            <dbReference type="Ensembl" id="ENSEASP00005007790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
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    <accession>A0A8C4LA22</accession>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
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                <person name="Sun"/>
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                <person name="J."/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="J."/>
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                <person name="G."/>
                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
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                <person name="Zhang"/>
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                <person name="Jin"/>
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                <person name="D.D."/>
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                <person name="X. and Zhong"/>
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            </authorList>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <property type="match status" value="1"/>
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                <person name="Guo"/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
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                <person name="Hou"/>
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                <person name="Pan"/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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                <consortium name="Ensembl"/>
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            <location evidence="1">Cytoplasm</location>
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    </dbReference>
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    </dbReference>
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    </evidence>
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    <accession>A0A8C4LGR4</accession>
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                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>K(+)(in) = K(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29463"/>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="23">Associates with KCNJ3/GIRK1 or KCNJ6/GIRK2 to form a G-protein-activated heteromultimer pore-forming unit. The resulting inward current is much larger.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 25">Membrane</location>
            <topology evidence="1 25">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ5 subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ6 subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014717519.1">
        <property type="nucleotide sequence ID" value="XM_014862033.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070344963.1">
        <property type="nucleotide sequence ID" value="XM_070488862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LGR4"/>
    <dbReference type="SMR" id="A0A8C4LGR4"/>
    <dbReference type="Ensembl" id="ENSEAST00005009195.2">
        <property type="protein sequence ID" value="ENSEASP00005008438.1"/>
        <property type="gene ID" value="ENSEASG00005006078.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106844532"/>
    <dbReference type="KEGG" id="eai:106844532"/>
    <dbReference type="CTD" id="3763"/>
    <dbReference type="GeneTree" id="ENSGT01080000257365"/>
    <dbReference type="OMA" id="SHEFNEH"/>
    <dbReference type="OrthoDB" id="273257at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 18"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990566">
        <property type="term" value="C:I(KACh) inward rectifier potassium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015467">
        <property type="term" value="F:G-protein activated inward rectifier potassium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990573">
        <property type="term" value="P:potassium ion import across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034765">
        <property type="term" value="P:regulation of monoatomic ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.70:FF:000019">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1400:FF:000005">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1400">
        <property type="entry name" value="G protein-activated inward rectifier potassium channel 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041647">
        <property type="entry name" value="IRK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016449">
        <property type="entry name" value="K_chnl_inward-rec_Kir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003275">
        <property type="entry name" value="K_chnl_inward-rec_Kir3.2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013518">
        <property type="entry name" value="K_chnl_inward-rec_Kir_cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040445">
        <property type="entry name" value="Kir_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767:SF19">
        <property type="entry name" value="G PROTEIN-ACTIVATED INWARD RECTIFIER POTASSIUM CHANNEL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11767">
        <property type="entry name" value="INWARD RECTIFIER POTASSIUM CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01007">
        <property type="entry name" value="IRK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17655">
        <property type="entry name" value="IRK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005465">
        <property type="entry name" value="GIRK_kir"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01328">
        <property type="entry name" value="KIR32CHANNEL"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01320">
        <property type="entry name" value="KIRCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15 25" id="KW-0407">Ion channel</keyword>
    <keyword evidence="13 25" id="KW-0406">Ion transport</keyword>
    <keyword evidence="14 27" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11 25" id="KW-0630">Potassium</keyword>
    <keyword evidence="7 25" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 25 27" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 27" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6 25" id="KW-0813">Transport</keyword>
    <keyword evidence="10 25" id="KW-0851">Voltage-gated channel</keyword>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="95"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="27">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Potassium channel inwardly rectifying transmembrane" evidence="28">
        <location>
            <begin position="59"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="Inward rectifier potassium channel C-terminal" evidence="29">
        <location>
            <begin position="205"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="394"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="406"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="site" description="Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium" evidence="24">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00031132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00031384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00031386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00032153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00032506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00034430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00064617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR005465-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="RuleBase" id="RU003822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="Pfam" id="PF17655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="425" mass="48684" checksum="2688D4448F4488D4" modified="2022-01-19" version="1">MTMAKLTESMTNVLEGDSMDQDVESPVAIHQPKLPKQARDDLPRHISRDRTKRKIQRYVRKDGKCNVHHGNVRETYRYLTDIFTTLVDLKWRFNLLIFVMVYTVTWLFFGMIWWLIAYIRGDMDHIEDPSWTPCVTNLNGFVSAFLFSIETETTIGYGYRVITDKCPEGIILLLIQSVLGSIVNAFMVGCMFVKISQPKKRAETLVFSTHAVISMRDGKLCLMFRVGDLRNSHIVEASIRAKLIKSKQTSEGEFIPLNQTDINVGYYTGDDRLFLVSPLIISHEINQQSPFWEISKAQLPKEELEIVVILEGMVEATGMTCQARSSYVTSEILWGYRFTPVLTLEDGFYEVDYNSFHETYETSTPSLSAKELAELASRAELPLSWSVSSKLNQHAELETEEEEKNLEEQTERNGDVAKLENESKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LK72</accession>
    <name>A0A8C4LK72_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Serine/threonine-protein kinase OSR1</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Oxidative stress-responsive 1 protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">OXSR1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="RefSeq" id="XP_070348416.1">
        <property type="nucleotide sequence ID" value="XM_070492315.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LK72"/>
    <dbReference type="SMR" id="A0A8C4LK72"/>
    <dbReference type="Ensembl" id="ENSEAST00005011532.2">
        <property type="protein sequence ID" value="ENSEASP00005010595.1"/>
        <property type="gene ID" value="ENSEASG00005007524.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106834262"/>
    <dbReference type="CTD" id="9943"/>
    <dbReference type="GeneTree" id="ENSGT00940000162134"/>
    <dbReference type="OMA" id="KMRTANC"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010820">
        <property type="term" value="P:positive regulation of T cell chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06610">
        <property type="entry name" value="STKc_OSR1_SPAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000043">
        <property type="entry name" value="serine/threonine-protein kinase OSR1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000114">
        <property type="entry name" value="serine/threonine-protein kinase OSR1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000068">
        <property type="entry name" value="STE20/SPS1-related proline-alanine-rich protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024678">
        <property type="entry name" value="Kinase_OSR1/WNK_CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE OSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12202">
        <property type="entry name" value="OSR1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0007">Acetylation</keyword>
    <keyword evidence="12 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="21">
        <location>
            <begin position="17"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="315"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="393"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="334"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00042936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005010595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="527" mass="58008" checksum="576E8737DC446B49" modified="2022-01-19" version="1">MSEDSSALPWSINRDDYELQEVIGSGATAVVQAAYCAPKKEKVAIKRINLEKCQTSMDELLKEIQAMSQCHHPNIVSYYTSFVVKDELWLVMKLLSGGSVLDIIKHIVAKGEHKSGVLDEPTIATILREVLEGLEYLHKNGQIHRDVKAGNILLGEDGSVQIADFGVSAFLATGGDITRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADIWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPSLETGVQDKEMLKKYGKSFRKMISLCLQKDPEKRPTAAELLRHKFFQKAKNKEFLQEKLLQRAPTISERAKKVRRVPGSSGRLHKTEDGGWEWSDDEFDEESEEGKAAISQLRSPRVKESLSNSELFPATDPVGTLLQVPEQISAHLPQPAGQMPAQPTQVSLPPPAEPAKTAQALSSGAGSQETKIPISLVLRLRNSKKELNDIRFEFTPGRDTAEGVSQELISAGLVDGRDLVIVAANLQKIVEEPQSNRSVTFKLASGVEGSDIPDDGKLIGFAQLSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LSJ1</accession>
    <name>A0A8C4LSJ1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cationic amino acid transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LSJ1"/>
    <dbReference type="Ensembl" id="ENSEAST00005017307.2">
        <property type="protein sequence ID" value="ENSEASP00005015921.2"/>
        <property type="gene ID" value="ENSEASG00005038122.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000154637"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002293">
        <property type="entry name" value="AA/rel_permease1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029485">
        <property type="entry name" value="CAT_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243:SF4">
        <property type="entry name" value="CATIONIC AMINO ACID TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43243">
        <property type="entry name" value="INNER MEMBRANE TRANSPORTER YGJI-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13520">
        <property type="entry name" value="AA_permease_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13906">
        <property type="entry name" value="AA_permease_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="109"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="196"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="264"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="310"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="457"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="485"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="524"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="551"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="domain" description="Cationic amino acid transporter C-terminal" evidence="9">
        <location>
            <begin position="521"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="414"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="414"/>
            <end position="426"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005015921.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="624" mass="66738" checksum="CF2DA74AD82B2EE3" modified="2023-09-13" version="2">RVPLHRAPSLARKLNRLKTLEDSTTKRSLRHHLTMLDLILLGMGTTGSVGLYVLVGTVAKEMAGPAVLVSFSMAAVASLLGALCYAEFGVCVPGRGSAYLFTYVSMGELWAFLVGWNTILGYLIGGAAVARVWSSYVDAIFSHRIRSFTMAHVGIWQVPFLAQYPDFLAAGVILLASALVSYGVGVSSWLNRTFSAINLVVILFIIILGFVLARPHNWSTEEGGFAPFGFSGIIDGAATCFFAFQGFGAIAASSMEAQNPKRAVPMAIAISLGLAAGTNILVSAALTFMVPWRSLDPDLALTDAFNQRGYSWAGFIVAAGAICAMNTFLLRTVIFLPRMVHAMAADGLFFQVFTYMHPQTLVPIVGIMVFGVLMAFLALLLDFKALVQFMSISALLNHTIVATSIVILRIHKSPPSSSLGSVSPVGTEQASAPEPRQLRPALSPYPSFPGGYRPEAAVAWALGVLVASAITLDCMLVFGGSALHLPPWGHTLLLLLSSVMFLLSLQIRPQASSPSLPLSPQVPMVPLTPALSILLNVFLMLHLSYLNWLSLSIWLLIGLVVYFGYGIWHSKENQRQLPGLTATCGGLEETVPVPGCGGPPAWVHASLRHRPTHSSPGGAHPCAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LVE3</accession>
    <name>A0A8C4LVE3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">OR10A7</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
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                <person name="M."/>
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                <person name="D.D."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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    <comment type="similarity">
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="Jin"/>
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                <person name="Yue"/>
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        <property type="match status" value="2"/>
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        <location>
            <begin position="507"/>
            <end position="528"/>
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    <feature type="domain" description="Ig-like" evidence="3">
        <location>
            <begin position="253"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="364"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="384"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="462"/>
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        <source>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005016379.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="573" mass="64131" checksum="2A2A4CFB68055963" modified="2023-09-13" version="2">MEKKWTYCAVYSIIQIHFVRGVWEEMFNAEEDVYALPGSDVNLTCQIRKKGFLVQMQWSKFTDKLDLIALYHPEYGSYCAIGSPCKSLVTYREISGNVSQWTLHLRNMSSSVTGKYECSFTLYPEGIWTKIYNLLIQTNVIQDEWRSNHTIEIEINRTLEIPCFQNISSEIASEFTFAWLVEHNGTQETLITQDRPISNSTLFKDRVRLGTDHGLRLSPVQIHDDHRKFSCHVMVRPGKIFRSTITVKVFAKPETPVIVENNSMDVLGKRIFNCSLRNVFPTANLTWFTDRSFPQGEREGRYITNEERKGKGGFLELKSVLTMVYGNKPAQSNNLTIWCTALSPAPGNKVWNISSEKITFSLGLADPPIDPPTSRTESALGTQPSPASSTSPTRSPAASSIALVDESTSTPNATPQTSDSNVTTQGFNYSWTSSGKDAKNREHNSASWMPSEMYSSSPSGADSTLHGDVFPSTTRTFSEVPKAASGSTKNNDIRITGIVVNKPKDGMSWPVIVAALVFSCVVVFGLGVRKWCQYQKEIMERPPPFKPPPPPIKYTCIQESIGCDLPCQEMETL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LW21</accession>
    <name>A0A8C4LW21_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aminomethyltransferase</fullName>
            <ecNumber evidence="11">2.1.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Glycine cleavage system T protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">AMT</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1 11">The glycine cleavage system catalyzes the degradation of glycine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>N(6)-[(R)-S(8)-aminomethyldihydrolipoyl]-L-lysyl-[protein] + (6S)-5,6,7,8-tetrahydrofolate = N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16945"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:10492"/>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="ChEBI" id="CHEBI:83143"/>
            <dbReference type="EC" id="2.1.2.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4 11">The glycine cleavage system is composed of four proteins: P, T, L and H.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the GcvT family.</text>
    </comment>
    <dbReference type="EC" id="2.1.2.10" evidence="11"/>
    <dbReference type="RefSeq" id="XP_014695903.1">
        <property type="nucleotide sequence ID" value="XM_014840417.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LW21"/>
    <dbReference type="SMR" id="A0A8C4LW21"/>
    <dbReference type="Ensembl" id="ENSEAST00005018034.2">
        <property type="protein sequence ID" value="ENSEASP00005016602.1"/>
        <property type="gene ID" value="ENSEASG00005011487.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106830686"/>
    <dbReference type="KEGG" id="eai:106830686"/>
    <dbReference type="CTD" id="275"/>
    <dbReference type="GeneTree" id="ENSGT00940000157524"/>
    <dbReference type="OMA" id="MPVQYPA"/>
    <dbReference type="OrthoDB" id="10263536at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005960">
        <property type="term" value="C:glycine cleavage complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004047">
        <property type="term" value="F:aminomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019464">
        <property type="term" value="P:glycine decarboxylation via glycine cleavage system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.110:FF:000002">
        <property type="entry name" value="Aminomethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.120:FF:000014">
        <property type="entry name" value="Aminomethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1400:FF:000001">
        <property type="entry name" value="Aminomethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.1250.10:FF:000002">
        <property type="entry name" value="Aminomethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.120:FF:000018">
        <property type="entry name" value="Aminomethyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.110">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1400">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1250.10">
        <property type="entry name" value="Aminomethyltransferase fragment"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006223">
        <property type="entry name" value="GcvT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028896">
        <property type="entry name" value="GcvT/YgfZ/DmdA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029043">
        <property type="entry name" value="GcvT/YgfZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013977">
        <property type="entry name" value="GcvT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006222">
        <property type="entry name" value="GCVT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00528">
        <property type="entry name" value="gcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001567">
        <property type="entry name" value="PRK00389.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43757:SF16">
        <property type="entry name" value="AMINOMETHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01571">
        <property type="entry name" value="GCV_T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08669">
        <property type="entry name" value="GCV_T_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006487">
        <property type="entry name" value="GcvT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101790">
        <property type="entry name" value="Aminomethyltransferase beta-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="GCVT N-terminal" evidence="12">
        <location>
            <begin position="38"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="domain" description="Aminomethyltransferase C-terminal" evidence="13">
        <location>
            <begin position="318"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="232"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR006487-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005016602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="403" mass="43715" checksum="FA3769B413642754" modified="2022-01-19" version="1">MQRAVSMVARLGLRLQAFPSTLGRPLSCTQDVLRRTPLYDFHLAHGGKMVAFAGWSLPVQYRDSHVDSHLHTRQHCSLFDVSHMLQTKIFGCDRVRLMESLVVGDIAELRPNQGTLSLFTNEAGGILDDLIVTNTSEGHLYVVSNAGCWDKDLALMQDKVRELQNIGSDVGLEVVDNALLALQGPTAAQVLQAGVADDLKKLPFMTSAVMEVFGVSGCRVTRCGYTGEDGVEISVPAVGAVRLATALLENPEVKLAGLAARDSLRLEAGLCLYGNDIDEHTTPVEGSLSWTLGKRRRAAMDFPGATVIVPQLKGKVQRRRVGLMCEGAPMRAHSPILSTEGTVIGTVTSGCPSPCLKKNVAMGYVPCEYSRPGTPLLVEVRQKQQMAVVSKMPFVPTNYYILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LZ76</accession>
    <name>A0A8C4LZ76_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Lysozyme like 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">LYZL6</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
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        <cofactor evidence="1">
            <name>Ca(2+)</name>
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        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00010859"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU004440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005015107.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="28115" checksum="74AE417EDDAB0FBD" modified="2023-09-13" version="2">MARALLLSLVSCLAAVSQASLIRRCDLAKVLHEEDLDGFEGYSLSDWLCLAFVQSNFNISKVNENTDGSFDYGIFQINSHYWCNDHKSHSENLCDVDCQGLAWAPGWERVAEPQSALDHQLCKKDRVQCRGDEELGRMEVALFGPAALLLDDRVPPGVRPCAGLLWSQPRTPLFAWEFFISSSSCLPVIFFPSRLQIKLTRAPGINGFLGLSPYFHPGPGPWFLTFANQEDHHDKVSVFLELLKHSLYKE</sequence>
</entry>
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    <accession>A0A8C4LZV1</accession>
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            <fullName evidence="8">Peptidase S1 domain-containing protein</fullName>
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        <name evidence="9" type="primary">LOC106848556</name>
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        <name type="scientific">Equus asinus</name>
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        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Equus</taxon>
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    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
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                <person name="X."/>
                <person name="Zhao"/>
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                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Wang"/>
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                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Li"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
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                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
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                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="gene ID" value="ENSEASG00005012262.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234551"/>
    <dbReference type="OMA" id="PRIDPYN"/>
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        <property type="component" value="Chromosome 2"/>
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        <property type="term" value="C:cytoplasm"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00190">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Complement factor D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.10">
        <property type="entry name" value="Trypsin-like serine proteases"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Peptidase_S1_PA"/>
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        <property type="entry name" value="Peptidase_S1_PA_chymotrypsin"/>
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    <dbReference type="InterPro" id="IPR001314">
        <property type="entry name" value="Peptidase_S1A"/>
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    <dbReference type="InterPro" id="IPR001254">
        <property type="entry name" value="Trypsin_dom"/>
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    <dbReference type="InterPro" id="IPR018114">
        <property type="entry name" value="TRYPSIN_HIS"/>
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    <dbReference type="PANTHER" id="PTHR24271">
        <property type="entry name" value="KALLIKREIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24271:SF22">
        <property type="entry name" value="MAST CELL PROTEASE 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00089">
        <property type="entry name" value="Trypsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00722">
        <property type="entry name" value="CHYMOTRYPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00020">
        <property type="entry name" value="Tryp_SPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50494">
        <property type="entry name" value="Trypsin-like serine proteases"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00134">
        <property type="entry name" value="TRYPSIN_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <keyword evidence="2 7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0865">Zymogen</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034837902" description="Peptidase S1 domain-containing protein" evidence="7">
        <location>
            <begin position="19"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S1" evidence="8">
        <location>
            <begin position="21"/>
            <end position="244"/>
        </location>
    </feature>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
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    <gene>
        <name evidence="18" type="primary">YAP1</name>
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            <taxon>Mammalia</taxon>
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    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
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                <person name="Q."/>
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                <person name="Y."/>
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                <person name="J."/>
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                <person name="R."/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
                <person name="J."/>
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                <person name="Yin"/>
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                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="18" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="18" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell junction</location>
            <location evidence="2">Tight junction</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="14">Belongs to the YAP1 family.</text>
    </comment>
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<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M0Q0</accession>
    <name>A0A8C4M0Q0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">E3 ubiquitin-protein ligase MARCHF3</fullName>
            <ecNumber evidence="5">2.3.2.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">Membrane-associated RING finger protein 3</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Membrane-associated RING-CH protein III</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="21">RING-type E3 ubiquitin transferase MARCHF3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="26" type="primary">MARCHF3</name>
    </gene>
    <organism evidence="26 27">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">E3 ubiquitin-protein ligase which may be involved in endosomal trafficking. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Early endosome membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="5"/>
    <dbReference type="RefSeq" id="XP_014715020.1">
        <property type="nucleotide sequence ID" value="XM_014859534.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M0Q0"/>
    <dbReference type="Ensembl" id="ENSEAST00005019673.2">
        <property type="protein sequence ID" value="ENSEASP00005018122.1"/>
        <property type="gene ID" value="ENSEASG00005012516.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106842815"/>
    <dbReference type="KEGG" id="eai:106842815"/>
    <dbReference type="CTD" id="115123"/>
    <dbReference type="GeneTree" id="ENSGT00940000159206"/>
    <dbReference type="OMA" id="FNDQPIC"/>
    <dbReference type="OrthoDB" id="273089at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16809">
        <property type="entry name" value="RING_CH-C4HC3_MARCH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000119">
        <property type="entry name" value="E3 ubiquitin-protein ligase MARCH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011016">
        <property type="entry name" value="Znf_RING-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46065">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCH 2/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46065:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE MARCHF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12906">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00744">
        <property type="entry name" value="RINGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51292">
        <property type="entry name" value="ZF_RING_CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="6" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="10" id="KW-0967">Endosome</keyword>
    <keyword evidence="15 23" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 23" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="14 23" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="11 22" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="23">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="RING-CH-type" evidence="25">
        <location>
            <begin position="63"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="24">
        <location>
            <begin position="71"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00037102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00040157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00041977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00043181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00043230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS51292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005018122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="253" mass="28521" checksum="6E08C605CEBC8A88" modified="2022-01-19" version="1">MTTSRCSHLPEVLPDCTSSAAPVVKTVEDCGSLVNGQPQYVMQVSAKDGQLLSTVVRTLATQSPFNDRPMCRICHEGSSQEDLLSPCECTGTLGTIHRSCLEHWLSSSNTSYCELCHFRFAVERKPRPLVEWLRNPGPQHEKRTLFGDMVCFLFITPLATISGWLCLRGAVDHLHFSSRLEAVGLIALTVALFTIYLFWTLVSFRYHCRLYNEWRRTNQRVILLIPKSVNVPSNQQSLLGLHSAKRNSKETIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4M2H4</accession>
    <name>A0A8C4M2H4_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Proline rich transmembrane protein 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">PRRT3</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_044609839.1">
        <property type="nucleotide sequence ID" value="XM_044753904.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070349934.1">
        <property type="nucleotide sequence ID" value="XM_070493833.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4M2H4"/>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Zhuang"/>
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                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
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                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MAG2"/>
    <dbReference type="Ensembl" id="ENSEAST00005024491.2">
        <property type="protein sequence ID" value="ENSEASP00005022571.2"/>
        <property type="gene ID" value="ENSEASG00005015417.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00400000024234"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038963">
        <property type="entry name" value="C17orf107"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38506">
        <property type="entry name" value="RIKEN CDNA 4930544D05 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38506:SF1">
        <property type="entry name" value="RIKEN CDNA 4930544D05 GENE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17688">
        <property type="entry name" value="DUF5536"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005022571.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="248" mass="26094" checksum="938E49F86A258A14" modified="2023-09-13" version="2">MKGTPSSLETLLWVYRFHSSTEVRLPQCPPELAPRDPLGRSPTPPPRPLPCDPIFLLQVALQPPLLSSLELAVAAAHEYLEQRFRELKSLEPKKPPTPKPTLGLVLREAAASVVSFGATLLEISALWLQQEVRRLDGGNGGLSPAPDGGDPGGALARVAEAAGQGARQVGAAAGASAGLLLQGAWLCLCGRGLHGSASFLQQWRRQLGLGTAGEPRYSGDLKVASSSSAEDGEPENPPPPHPTPRPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MAW7</accession>
    <name>A0A8C4MAW7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">USP30</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
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                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4MAW7"/>
    <dbReference type="Ensembl" id="ENSEAST00005023038.2">
        <property type="protein sequence ID" value="ENSEASP00005021218.2"/>
        <property type="gene ID" value="ENSEASG00005014534.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00550000075075"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008053">
        <property type="term" value="P:mitochondrial fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035871">
        <property type="term" value="P:protein K11-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044313">
        <property type="term" value="P:protein K6-linked deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02662">
        <property type="entry name" value="Peptidase_C19F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF888">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="95"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="10">
        <location>
            <begin position="128"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="423"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005021218.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="576" mass="64648" checksum="DE65F1E417FECCF0" modified="2023-09-13" version="2">MWSVGSCSPAAAASGPLGSRSFGLLSPEELGVWLRVPPPREPAEPWVRPRRGRPAFRGAAMLSSRAEAAVTAADRAIQRFLRTGAAVRYKVMKNWGVIGGIAAALAAGIYVIWGPITERKKRRKGLVPGLVNLGNTCFMNSLLQGLSACPAFIRWLEEFTSQYARDQMEPPPHQYLSLTLLHLLKALSCQEVTDDEVLDASCLLDVLRMYRWQISSFEEQDAHELFHVITSSLEDERDRQPRVTHLFDVHSLEQSEATPKQITCRTRGSLHPTFTHWKSQHPFHGRLTSNMVCKHCEHQSPVRFDTFDSLSLSIPAATWGHPLTLDHCLHHFISSESVRDVVCDNCTKIEAKGTLNGEKVEHQRTTFVKQLKLGKLPQCLCIHLQRLSWSSHGTPLKRHEHVQFNEFLMMDIYKYHLLGHKPGQRGPKLKENPGPAVELQDGPAAPRSVLNQPGGPKTQIFMNGACSPSLLPTLPTPMPFPLPVLPDYSSSSYLFRLMAVVVHHGDMHSGHFVTYRRSPPSAKNPLSTSSQWLWISDDSVRKASLQEVLSSSAYLLFYERVLSRTQQQSPEYKSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MCW0</accession>
    <name>A0A8C4MCW0_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Synaptic vesicle 2-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SVOP</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasmic vesicle</location>
            <location evidence="1">Secretory vesicle</location>
            <location evidence="1">Synaptic vesicle membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014723292.1">
        <property type="nucleotide sequence ID" value="XM_014867806.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MCW0"/>
    <dbReference type="Ensembl" id="ENSEAST00005023025.2">
        <property type="protein sequence ID" value="ENSEASP00005021205.1"/>
        <property type="gene ID" value="ENSEASG00005014529.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106848087"/>
    <dbReference type="KEGG" id="eai:106848087"/>
    <dbReference type="CTD" id="55530"/>
    <dbReference type="GeneTree" id="ENSGT00940000155403"/>
    <dbReference type="OMA" id="LLWFIWM"/>
    <dbReference type="OrthoDB" id="4139357at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030672">
        <property type="term" value="C:synaptic vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17441">
        <property type="entry name" value="MFS_SVOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000084">
        <property type="entry name" value="Synaptic vesicle 2-related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004749">
        <property type="entry name" value="Orgcat_transp/SVOP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047969">
        <property type="entry name" value="SVOP-like_MFS_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00898">
        <property type="entry name" value="2A0119"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511:SF5">
        <property type="entry name" value="MAJOR FACILITATOR-TYPE TRANSPORTER HXNZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23511">
        <property type="entry name" value="SYNAPTIC VESICLE GLYCOPROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0770">Synapse</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005021205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="548" mass="60724" checksum="A844748CFC8FFFB8" modified="2022-01-19" version="1">MEEDLFQLRQLPVVKFRRTGESARSEDDTASGEHEVQIEGVRTGLEAVELDDGAAVPKEFANPTDDTFMVEDAVEAIGFGKFQWKLSVLTGLAWMADAMEMMILSILAPQLHCEWRLPSWQVALLTSVVFVGMMSSSTLWGNISDQYGRKTGLKISVLWTLYYGVLSAFAPVYSWILVLRGLVGFGIGGVPQSVTLYAEFLPMKARAKCILLIEVFWAIGTVFEVVLAVFVMPSLGWRWLLILSAVPLLLFAVLCFWLPESARYDVLSGNQEKAIATLKRIATENGAPMPLGKLIVSRQEDRGKMRDLFTPHFRWTTLLLWFIWFSNAFSYYGLVLLTTELFQAGDVCSISSRKKAVEAKCSLACEYLSEEDYMDLLWTTLSEFPGVLVTLWIIDRLGRKKTMALCFAIFSLCSLLLFICVGRNMLTLLLFIARAFISGGFQAAYVYTPEVYPTATRALGLGSCSGMARVGALITPFIAQVMLESSVYLTLAVYSGCCLLAALASCFLPIETKGRGLQESSHREWGQEMVGRGTLGTGVTRSNSGSQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MFW9</accession>
    <name>A0A8C4MFW9_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Zinc finger protein 286A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ZNF286A</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014719699.1">
        <property type="nucleotide sequence ID" value="XM_014864213.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_014719700.1">
        <property type="nucleotide sequence ID" value="XM_014864214.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MFW9"/>
    <dbReference type="Ensembl" id="ENSEAST00005027528.2">
        <property type="protein sequence ID" value="ENSEASP00005025353.2"/>
        <property type="gene ID" value="ENSEASG00005017262.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106845790"/>
    <dbReference type="KEGG" id="eai:106845790"/>
    <dbReference type="CTD" id="57335"/>
    <dbReference type="GeneTree" id="ENSGT00940000161686"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07765">
        <property type="entry name" value="KRAB_A-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000040">
        <property type="entry name" value="RB associated KRAB zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000424">
        <property type="entry name" value="Zinc finger protein 140"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001089">
        <property type="entry name" value="zinc finger protein 140 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001337">
        <property type="entry name" value="Zinc finger protein 286A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001434">
        <property type="entry name" value="zinc finger protein 286A isoform X1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002343">
        <property type="entry name" value="Zinc finger protein 33A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000016">
        <property type="entry name" value="zinc finger protein 37 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:002090">
        <property type="entry name" value="Zinc finger protein 473"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000737">
        <property type="entry name" value="Zinc finger protein 565"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001909">
        <property type="entry name" value="KRAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036051">
        <property type="entry name" value="KRAB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF178">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01352">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00349">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109640">
        <property type="entry name" value="KRAB domain (Kruppel-associated box)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50805">
        <property type="entry name" value="KRAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="KRAB" evidence="15">
        <location>
            <begin position="45"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="243"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="271"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="326"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="354"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="382"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="410"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="438"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="466"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="494"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
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        <source>
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            <dbReference type="ARBA" id="ARBA00023015"/>
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            <dbReference type="ARBA" id="ARBA00023125"/>
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            <dbReference type="ARBA" id="ARBA00023163"/>
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            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
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    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
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            <dbReference type="PROSITE" id="PS50805"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="521" mass="60230" checksum="ED578BCD5F854CDA" modified="2023-09-13" version="2">MDTDLAEMPEKRVLSSQDSSFSQEKSTEEGEVAALRLTARSQASMTFKDVAMDFTPEEWGKLDPAQRDVMLENYRNLVSLWLPVSKPENYYLENAKEPLMLERKAPKSGYSDLETSPESKDATSVQDFSKEESCQVAIIDRLTRNSVYDSNLETTLECENWLEDQQGNQERHLREMFTHMNSLSEERAHEHDVYWKNCSQKSVLLTQDRGPKGSYAFHTLENRLKQKSNLMKKQRTYKEKKPHKCNDCGELFTYHSVLIRHQRVHTGEKPYSCNECGKSFSHRANLTKHQRTHTRILFECSECKKAFTESSSLAIHQRIHIGERPYECGECGKGFNRSTHLVQHQLIHTGVKPYECNECDKAFIHSSALIKHQRTHTGEKPYKCQECGKAFSHCSSLTKHQRVHTGEKPYECSECGKTFSQSTHLVQHQRIHTGEKPYECNECGKTFSRSSNFAKHQRIHIGKKPYKCNECGKAFIHSSALIQHQRTHTGEKPYRCNECGKSFKCSSSLIRHQRIHTEEQP</sequence>
</entry>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Tian"/>
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                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Gao"/>
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                <person name="Ju"/>
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                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
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                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="439" mass="46631" checksum="43045288E2C97230" modified="2023-09-13" version="2">MGQSSVWGVLVTLGIQETMLSGAAGCARLEGLAGYPQTALGKEPQPPALLTLKHGMETGKPCHCHFKSPEEGGPSPTAHHTEVSERRKWGRVWRTPSPAAPPACSHLQTSCNETGNRCRPHHHSPQSLGQRGAMDRPRALLLLLALLTLCITAGTPKVWVQVQTEAAKSPSFTVRCGFLGSGSISLVTVSCGKPDGAEGASLAVLHPEFGTELKAPARQAHWETKTSISLTLEGSEGRSPSPNTTFCCKFVSFPEGSQAACGNPSLSTDQGLSASTPAPVVRADLAGIWGVSGVLLFGCFYLLHLLRRQRHWSVMKLQPPLSSPQTQMPARAAGPASLASLHIPYATVNTSYFCPATLDTVLPPQPLSRWAQLPTHRTRQPQAPAPWASLPASARSSFISVENGLYTQAGERPPHAGPNLTPFPDPLGPRAVEGRLGVR</sequence>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Zhang"/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="A."/>
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                <person name="D.D."/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        </location>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    <sequence length="853" mass="98191" checksum="3123BA6F81C9693C" modified="2023-09-13" version="2">MALRLAADFDLRKDVLPGLRAAAGARGGGAGILENNYESLRVLNVERNGNIIYTYKDDKGNVFFGLFDCHTRQNEHLYTFEKDLQVVSCSVNRERTLLAASLVQSTKEGRRNELQPGSKCLTLLVEIHPVNNVKVLKAVDSYIWVQFLHPHVESNPLPENHLLLISEEKYIEQFHIQVIQEDGNGVVIKNSGHLPRERVAEDFVWAQWDMSEQRLYYVELKKSRSILKCIQFYADENFNLMFEAPLDIALSDSGFNLVNFGCTDLQDQEKLSEHPTLCVFTNHTGSLCVCYSPKLDSWEQITYSVFYFHKGHSKTFTAALGSADSRVTKGITFLNLDYFVAVYLPGHFFHLLNIQHPDLVCHSLFLTGNNEVVDMLPHGPLQSLSGSLVLDWCSGRLYRADLNRSYLLEFLWDARLDWEKMAVLHCVLSCRQDTRFLEAKIIQWISENISACHSFDLIQEFIIASSYWSIYPETGNMDKLLPYSSVLTWNTEIPGITLVTEEIALPLMRVHSFKGYWEKLHSNLEYVKYSEPHSHYSNSVVRREWHSLISEEKTGKRRSMAYVRNILDNAIKVISKLEARRLEPRLTPLFQEEDNHQRLLVGLMVSELKDHLLRHLPGIEKKRIEQMVLDYISKQLDLICQILEASWRRHNLHPWIFHFNRRASAAEFAVFHIMTRILEATHSLFLPLPPGFHTLHTVLGVRCLPLHNLLHYIDNGVLLLTETAVTRLMKDLDNTEKSEKLKFSIIVRLPPFEVHSKITWKICTLRVHPVSSNIISRNHVKRLLQDYKKQPRSSVIDKSSYSVEFLPLNYFTGILTDIESSSQALNAFEGHDNVDAKFVEEAALKHTTVLLGL</sequence>
</entry>
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    <accession>A0A8C4MPD4</accession>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Zhao"/>
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                <person name="Wang"/>
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                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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                <consortium name="Ensembl"/>
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        <citation type="submission" date="2025-09" db="UniProtKB">
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                <consortium name="Ensembl"/>
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        <source>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="11">Selenoprotein with thioredoxin reductase-like oxidoreductase activity. Protects dopaminergic neurons against oxidative stress and cell death. Involved in ADCYAP1/PACAP-induced calcium mobilization and neuroendocrine secretion. Plays a role in fibroblast anchorage and redox regulation. In gastric smooth muscle, modulates the contraction processes through the regulation of calcium release and MYLK activation. In pancreatic islets, involved in the control of glucose homeostasis, contributes to prolonged ADCYAP1/PACAP-induced insulin secretion.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the SelWTH family. Selenoprotein T subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4MS57"/>
    <dbReference type="Ensembl" id="ENSEAST00005034145.2">
        <property type="protein sequence ID" value="ENSEASP00005031412.1"/>
        <property type="gene ID" value="ENSEASG00005021389.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000011725"/>
    <dbReference type="OMA" id="LKFQICC"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 21"/>
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    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035773">
        <property type="term" value="P:insulin secretion involved in cellular response to glucose stimulus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031016">
        <property type="term" value="P:pancreas development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000085">
        <property type="entry name" value="Selenoprotein T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011893">
        <property type="entry name" value="Selenoprotein_Rdx-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019389">
        <property type="entry name" value="Selenoprotein_T"/>
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    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02174">
        <property type="entry name" value="CXXU_selWTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13544">
        <property type="entry name" value="SELENOPROTEIN T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13544:SF0">
        <property type="entry name" value="THIOREDOXIN REDUCTASE-LIKE SELENOPROTEIN T"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF10262">
        <property type="entry name" value="Rdx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
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    <keyword evidence="9 12" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0712">Selenocysteine</keyword>
    <keyword evidence="3 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034464002" description="Selenoprotein T" evidence="12">
        <location>
            <begin position="20"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007551"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022933"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048132"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00059375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005031412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="189" mass="21460" checksum="287D0DD8820E0CF4" modified="2022-01-19" version="1">MRLLLLLAVAASAVLRSDASGSLGGVPGKRLKMQYATGPLLKFQIWRVFEEYMRVISQRYPDIRIEGENYLPQPIYRHIASFLSVFKLVLIGLIIVGKDPFAFFGMQAPSIWQWGQENKVYACMMVFFLSNMIENQCMSTGAFEITLNDVPVWSKLESGHLPSMQQLVQILDNEMKLNVHMDPIPHHGS</sequence>
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        <submittedName>
            <fullName evidence="2">Small integral membrane protein 32</fullName>
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        <name evidence="2" type="primary">SMIM32</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
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            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
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            <taxon>Equus</taxon>
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    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
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                <person name="Fan"/>
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                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="103" mass="10889" checksum="B247BFCF91C18788" modified="2022-01-19" version="1">MYGDVFNATGGPEAAVGGALALAATVKAEGALPLELATARGMRDGAATKPDLPTYLLLFFLLLLSVALVILFIGCQLRHSAFAALPHDRSLRDARAPWKMRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MUY0</accession>
    <name>A0A8C4MUY0_EQUAS</name>
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        <recommendedName>
            <fullName evidence="19">Sodium channel regulatory subunit beta-1</fullName>
        </recommendedName>
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        <name evidence="24" type="primary">SCN1B</name>
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    <organism evidence="24 25">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
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            <taxon>Mammalia</taxon>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="24 25" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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                <person name="Sun"/>
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                <person name="Zhao"/>
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                <person name="S."/>
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                <person name="Wu"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="24" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="24" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Regulatory subunit of multiple voltage-gated sodium (Nav) channels directly mediating the depolarization of excitable membranes. Navs, also called VGSCs (voltage-gated sodium channels) or VDSCs (voltage-dependent sodium channels), operate by switching between closed and open conformations depending on the voltage difference across the membrane. In the open conformation they allow Na(+) ions to selectively pass through the pore, along their electrochemical gradient. The influx of Na+ ions provokes membrane depolarization, initiating the propagation of electrical signals throughout cells and tissues. The accessory beta subunits participate in localization and functional modulation of the Nav channels. Modulates the activity of SCN1A/Nav1.1, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN4A/Nav1.4, SCN5A/Nav1.5, SCN8A/Nav1.6, SCN9A/Nav1.7 and SCN10A/Nav1.8.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cell projection</location>
            <location evidence="3">Axon</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Perikaryon</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the sodium channel auxiliary subunit SCN1B (TC 8.A.17) family.</text>
    </comment>
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    <dbReference type="Ensembl" id="ENSEAST00005034166.2">
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        <property type="gene ID" value="ENSEASG00005021402.2"/>
    </dbReference>
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        <property type="component" value="Chromosome 26"/>
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        <property type="term" value="C:intercalated disc"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0033268">
        <property type="term" value="C:node of Ranvier"/>
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    </dbReference>
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        <property type="term" value="C:perikaryon"/>
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        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
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        <property type="term" value="C:T-tubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001518">
        <property type="term" value="C:voltage-gated sodium channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019871">
        <property type="term" value="F:sodium channel inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="F:transmembrane transporter binding"/>
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        <property type="term" value="P:cardiac muscle cell action potential involved in contraction"/>
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        <property type="term" value="P:corticospinal neuron axon guidance"/>
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        <property type="entry name" value="Immunoglobulins"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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        <property type="entry name" value="Ig-like_fold"/>
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    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027098">
        <property type="entry name" value="Na_channel_b1/b3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10546:SF2">
        <property type="entry name" value="SODIUM CHANNEL SUBUNIT BETA-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10546">
        <property type="entry name" value="SODIUM CHANNEL SUBUNIT BETA-1 AND 3"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="16" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 21" id="KW-0472">Membrane</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 22" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0915">Sodium</keyword>
    <keyword evidence="14" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="6 21" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 21" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="22">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
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    <feature type="chain" id="PRO_5040353698" description="Sodium channel regulatory subunit beta-1" evidence="22">
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            <begin position="20"/>
            <end position="284"/>
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            <begin position="161"/>
            <end position="182"/>
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            <begin position="24"/>
            <end position="134"/>
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            <dbReference type="ARBA" id="ARBA00022475"/>
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            <dbReference type="ARBA" id="ARBA00022692"/>
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            <dbReference type="ARBA" id="ARBA00022729"/>
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            <dbReference type="Proteomes" id="UP000694387"/>
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    <sequence length="284" mass="31743" checksum="A01F10822F0D803B" modified="2023-09-13" version="2">MGTLLAFVVGTALVSSAWAGCVEVDSETEAVYGMTFKILCISCKRRSETTAETFTEWTFRQKGTQEFVKILRYENEVLQLEEDERFEGRVVWNGSRGTKDLQDLSIFITNVTYNHSGDYQCHVYRLLFFDNYEHNTSVVKKIHLEVVDKANRDMASIVSEVMMYVLIVVLTIWLVAEMVYCYKKIAAATEAAAQENASEYLAITSESKENCTGVQALGFTSRKSQPYWETPGHSLPPMGVGHSWASTSLRDLPMEPPGWEGAGGLADTPMQPSFSGPIAHPRHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MXE1</accession>
    <name>A0A8C4MXE1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Anoctamin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ANO4</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="8">Membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the anoctamin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014690559.1">
        <property type="nucleotide sequence ID" value="XM_014835073.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MXE1"/>
    <dbReference type="Ensembl" id="ENSEAST00005031895.2">
        <property type="protein sequence ID" value="ENSEASP00005029348.1"/>
        <property type="gene ID" value="ENSEASG00005019948.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106827284"/>
    <dbReference type="KEGG" id="eai:106827284"/>
    <dbReference type="CTD" id="121601"/>
    <dbReference type="GeneTree" id="ENSGT00940000158600"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005229">
        <property type="term" value="F:intracellularly calcium-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032394">
        <property type="entry name" value="Anoct_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007632">
        <property type="entry name" value="Anoctamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049452">
        <property type="entry name" value="Anoctamin_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308">
        <property type="entry name" value="ANOCTAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12308:SF28">
        <property type="entry name" value="ANOCTAMIN-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16178">
        <property type="entry name" value="Anoct_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04547">
        <property type="entry name" value="Anoctamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="350"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="426"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="502"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="548"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="597"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="717"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="767"/>
            <end position="791"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin dimerisation" evidence="12">
        <location>
            <begin position="111"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="Anoctamin transmembrane" evidence="11">
        <location>
            <begin position="339"/>
            <end position="920"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="72"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="919"/>
            <end position="953"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005029348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="955" mass="111429" checksum="93715F153E4654D3" modified="2022-01-19" version="1">MEASSSGITNGKTKVFHPEGGVDLQGYQLDMQILPDGPKSDVDFSEILNAIQEMAKDVNILFDELEAVNSPCKDDDSLLHPGNLTSTSDDASRLEAGGETMPERNKSNGLYFRDGKCRIDYILVYRKSNPQTEKREVFERNIRAEGLHMEKESSLINSDIIFVKLHAPWEVLGRYAEQMNVRMPFRRKIYYLPRRYKFMSRIDKQISSFRRWLPKKPMRLDKETLPDLEENDCYTAPFSQQRIHHFIIHNKDTFFNNATRSRIVHHILQRIKYEEGKNKIGLNRLLTNGSYEAAFPLHEGGYRSKNSIRTHGAENHRHLLYECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAFIGLFVFLYGVITLDHCQVSKEVCQATDIIMCPVCDKYCPFMRLSDSCVYAKVTHLFDNGATVFFAVFMAVWATVFLEFWKRRRAVIAYDWDLIDWEEEEEEIRPQFEAKYSKKERMNPISGKPEPYQAFADKCSRLVVSASGIFFMICVVIAAVFGIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYEKVALLLTNLEQPRTESEWENSFTLKMFLFQFVNLNSSTFYIAFFLGRFTGHPGAYLRLINRWRLEECHPSGCLIDLCMQMGIIMVLKQTWNNFMELGYPLIQNWWTRRKVRQEHGPERRISFPQWEKDYNLQPMNAYGLFDEYLEMILQFGFTTIFVAAFPLAPLLALLNNIIEIRLDAYKFVTQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAITSDFIPRLVYAYKYGPCAGQGEAGQKCMVGYVNASLSVFRISDFENRSEPESDGSEFSGTPLKYCRYRDYRDPPHSLVPYGYTLQFWHVLAARLAFIIVFEHLVFCIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERLQKERKERKKNGKAHHNEWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4MYX4</accession>
    <name>A0A8C4MYX4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="14">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">POMT2</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="function">
        <text evidence="13">Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient. Essentially dedicated to O-mannosylation of alpha-DAG1 and few other proteins but not of cadherins and protocaherins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="14"/>
    <dbReference type="RefSeq" id="XP_014696387.2">
        <property type="nucleotide sequence ID" value="XM_014840901.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4MYX4"/>
    <dbReference type="Ensembl" id="ENSEAST00005032067.2">
        <property type="protein sequence ID" value="ENSEASP00005029508.2"/>
        <property type="gene ID" value="ENSEASG00005020051.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106830956"/>
    <dbReference type="KEGG" id="eai:106830956"/>
    <dbReference type="CTD" id="29954"/>
    <dbReference type="GeneTree" id="ENSGT00940000156829"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 7"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071711">
        <property type="term" value="P:basement membrane organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021542">
        <property type="term" value="P:dentate gyrus development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0150103">
        <property type="term" value="P:reactive gliosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd23282">
        <property type="entry name" value="beta-trefoil_MIR_POMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000026">
        <property type="entry name" value="Blast:Protein O-mannosyl-transferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF46">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="209"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="293"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="346"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="663"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="707"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="736"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="768"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="15">
        <location>
            <begin position="401"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="15">
        <location>
            <begin position="470"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="15">
        <location>
            <begin position="531"/>
            <end position="588"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00046005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005029508.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="817" mass="91603" checksum="89D77022247EF2F6" modified="2023-09-13" version="2">MLCATSRFLAATALRAQPRGRGPALRCRRRELHFPGHPEPPLCDFLTGQLGRPSVPAGRRAILRRGRMPPAVGGGLAGSELRARRGRCGSQAARAVSPDVAVEAAARSHKQPAPGSRHFEAAGRWALLALVTLLSFATRFHRLDEPQHICWDETHFGKMGSYYINRTFFFDVHPPLGKMLIGLAGYLSGYDGTFSFQKPGDKYEHHNYMGMRGFCASLGSLLVPFAYLTVLELSKSLPAALLTAALLTFDTGCLTLSQYILLDPILMFFIMAAMLSMVKYNSCADRPFSAPWWFWLSLTGINLAGALGVKFVGLFIIVQVGWNTISDLWHLFGDLSLSLVTVGKHLTARILCLIVLPLALYTATYAVHFMVLNKSGPGDGFFSSAFQARLSGNNLHNASIPEYLAYGSVITVKNLRMAIGYLHSHRHLYPEGIGARQQQVTTYLHKDYNNLWIIKKHNTNSDPLDPSFPVEFVRHGDIIRLEHKETSRNLHSHYHEAPLTRKHYQVTGYGINGTGDSNDFWRIEVVNRKFGNRIKVLRSRIRFIHLVTGCVLGSSGKVLPKWGWEQLEVTCTPYLKETLNSIWNVEDHINPKLPNISLDVLQPSFPEILLESHMVMIRGNNGLKPKDNEFTSKPWHWPINYQGLRFSGINDTDFRVYLLGNPVVWWLNLLSIALYLLSGSIIAVAMQRGARLPPEVEGLSQVLLRGGGQLLLGWMLHYFPFFLMGRILYFHHYFPAMLFSSMLTGVLWDTLLRLCAWSLASSSLARGIHTVGIMSLLLGAAYSFYLFHPLAYGMVGPLAQDPRSPMAGLRWLESWDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N0P3</accession>
    <name>A0A8C4N0P3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">RPS24</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="6" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS24 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4N0P3"/>
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        <property type="protein sequence ID" value="ENSEASP00005033687.1"/>
        <property type="gene ID" value="ENSEASG00005023056.2"/>
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    <dbReference type="GeneTree" id="ENSGT00390000000153"/>
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        <property type="component" value="Chromosome 28"/>
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        <property type="term" value="C:cytosolic small ribosomal subunit"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="F:structural constituent of ribosome"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="FunFam" id="3.30.70.3370:FF:000001">
        <property type="entry name" value="40S ribosomal protein S24"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="3.30.70.3370">
        <property type="match status" value="1"/>
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    <dbReference type="HAMAP" id="MF_00545">
        <property type="entry name" value="Ribosomal_eS24"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR053709">
        <property type="entry name" value="eRP_eS24_sf"/>
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    <dbReference type="InterPro" id="IPR001976">
        <property type="entry name" value="Ribosomal_eS24"/>
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    <dbReference type="InterPro" id="IPR018098">
        <property type="entry name" value="Ribosomal_eS24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
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    <dbReference type="PANTHER" id="PTHR10496">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01282">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00529">
        <property type="entry name" value="RIBOSOMAL_S24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="119"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005033687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="211" mass="23926" checksum="D3E474AD7DFEC7B9" modified="2022-01-19" version="1">MNDTVTIRTRKFMTNRLLQRKQMVIDVLHPGKATVPKTEIREKLAKMYKTTPDVIFVFGFRTHFGGGKTTGFGMIYDSLDYAKKNEPKHRLARVGLLIYCTAAEDVFSVMLCCGFSGMSKSKQSGEGQHKHLKNSEGVQRKEGDERGSLIKYRGSEVGSAAFEEVAFELGLEGQEKWCLWRWGLEAGGEFGKLGWYQIEILITKCEWTKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4N183</accession>
    <name>A0A8C4N183_EQUAS</name>
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        <recommendedName>
            <fullName evidence="19">EF-hand calcium-binding domain-containing protein 6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20">DJ-1-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="23" type="primary">EFCAB6</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="17">Negatively regulates the androgen receptor by recruiting histone deacetylase complex, and protein DJ-1 antagonizes this inhibition by abrogation of this complex. Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating.</text>
    </comment>
    <comment type="subunit">
        <text evidence="18">Microtubule inner protein component of sperm flagellar doublet microtubules. Binds PARK7. Part of a ternary complex containing PARK7, EFCAB6/DJBP and AR.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell projection</location>
            <location evidence="2">Cilium</location>
            <location evidence="2">Flagellum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
            <location evidence="3">Cytoskeleton</location>
            <location evidence="3">Cilium axoneme</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="Ensembl" id="ENSEAST00005034901.2">
        <property type="protein sequence ID" value="ENSEASP00005032047.2"/>
        <property type="gene ID" value="ENSEASG00005021875.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000013629"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160111">
        <property type="term" value="C:axonemal A tubule inner sheath"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036126">
        <property type="term" value="C:sperm flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030317">
        <property type="term" value="P:flagellated sperm motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000242">
        <property type="entry name" value="EF-hand calcium binding domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000243">
        <property type="entry name" value="EF-hand calcium binding domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000325">
        <property type="entry name" value="EF-hand calcium binding domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000492">
        <property type="entry name" value="EF-hand calcium binding domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000121">
        <property type="entry name" value="EF-hand calcium-binding domain-containing protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000179">
        <property type="entry name" value="EF-hand calcium-binding domain-containing protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000240">
        <property type="entry name" value="EF-hand calcium-binding domain-containing protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000285">
        <property type="entry name" value="EF-hand calcium-binding domain-containing protein 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015070">
        <property type="entry name" value="EF_hand_DJBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052603">
        <property type="entry name" value="EFCB6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20875:SF2">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20875">
        <property type="entry name" value="EF-HAND CALCIUM-BINDING DOMAIN-CONTAINING PROTEIN 6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08976">
        <property type="entry name" value="EF-hand_11"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13833">
        <property type="entry name" value="EF-hand_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="16" id="KW-0966">Cell projection</keyword>
    <keyword evidence="12" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="14" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-0282">Flagellum</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0678">Repressor</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="11" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="EF-hand" evidence="22">
        <location>
            <begin position="97"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="22">
        <location>
            <begin position="199"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="22">
        <location>
            <begin position="774"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="22">
        <location>
            <begin position="908"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="22">
        <location>
            <begin position="989"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="22">
        <location>
            <begin position="1094"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="22">
        <location>
            <begin position="1459"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="608"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1269"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="612"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1285"/>
            <end position="1299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00054968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00063636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00069150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00083181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005032047.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1521" mass="174735" checksum="9A88FF8DEA6328FF" modified="2023-09-13" version="2">MKRNIAKTNVAPVNTSKSTNTKARDLMCKMAIIPDWQISYPHTRKFTSLRPHSSPCRVDLRNSFQNVFRPSSSTTALANPILSFLDVKRILFQKITDKGDELKKAFQLLDTSQSMTVSKSDLRRVVTTFLLPLTREQFQDVLAQIPLTSSGAVPYNEFLSRFGGIDLNISVIKRGDGNETNCCRTLGELEIQVGEKIFKNIKTVMKALKLIDVNKTGLVQARELRRVLETFCLKMKDDEYKKFAKHYNIDTDTAVDYNAFLKNLSVNNALNLRYHMGDQEVSWENQQIKSSRRERLPSSISSEEVWRDYSLDEIERAFCQELSKSYEKVEKALSAGDPSKAGHVSLNYLKVVLDTFVYQLPRRIFIQLMKRFGLKTTAKVNWKQFLTSLYESPWLEVNNIVPPTKRNSINSRNKSCKENIITKLFRHEDRYTSLKKALLLTNAQPDGQITGEELRHILNCMVVKISDSEFKELMQILDPGGTGCVNVNTFGELLEEKAATGKTSSCADTKAPLLLAWDSVEEIVHDAITRNLQAFYNMLRSYDLGDTGLIGRNNFKKILRIFCPFLTTEHLIKLCSKFHDMASGRILYKKLLTCLGIKGPPAISPVLVPKDQLSKEQPQKEEEQQPDLSERAKPAESKITATRKMTKEEVIEKLKSCIRRQDPVFRRRFLDVSKELHHKINAHDFKKVLEDHGMPMDDDQYALLTAKIGFKKEGMSYLDFATGFEGARVQEREVTPSQTPVPAKSNLDSFVTAEECLRQFPQRLKESFRGPYSAFFEMDTDRDGIVSMLNLHQLLQRLLLNLRDEEFERFLGLLGLRLSTTLNFREFQNLCEKRPLRTDDAPQRLIRPKQKVADSELACEQAHQYLVTKAKTRWSDLSKNFIETDNEGNGILRRRDIKNALYGFDIPLTPREFEKLWMSYDTEGRGHITYQEFLQKLGISYSADVHQPYAEDYFNFMGHFTKPQQVQEEIKELQQSTEKAVPAREKLKDHYQDISKAVTKLDKSKNGYVSLSSMQKVLQECGCPLREEELTDLLSSWGISWHDNSINYLDFLKAVENSKPTRPQPEEKEESMPINFATLNPEDVLKSIQEVVASSGPALSTAFSALDKEDTGFVKASEFGQVLKDFCYKLTDSQYHYFLRKLRIHLTPHVNWKYFLQNFGDFLEETAAEWAEKMPKGPPPRSSKEMANREILARLHKAVTSHYHTIAQEFENFDTMKTHTASRDEFRSICTRHVQVLTDEQFDRLWNEMPVNAKGRLKYLDFLSRFSSERAATPPDTDDSAKAQRGSSVPNVSAESSPTGDPKAGSKPRSHPCTPASAILEPSAQPLQNCEPIETKLRKRIQSCWREFLRECKERDVNKQGEITAPEFLALVEKFSLDVNRAECQQLLVKYDLKNSGRFAYCDFIQSCVLLLKAKETSVMQRMKIQNAHKMKEAGAETSSFYAALLRIQPKIVHCWRPMRRTFKAYDEGSTGLLSVADFRQVLRQYSVNLSEEEFFHILEYYDKTLTSKISYNDFLRAFLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PGH2</accession>
    <name>A0A8C4PGH2_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Astrocytic phosphoprotein PEA-15</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">15 kDa phosphoprotein enriched in astrocytes</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">PEA15</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PGH2"/>
    <dbReference type="Ensembl" id="ENSEAST00005003531.2">
        <property type="protein sequence ID" value="ENSEASP00005003227.2"/>
        <property type="gene ID" value="ENSEASG00005002499.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000000230"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000165">
        <property type="term" value="P:MAPK cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046325">
        <property type="term" value="P:negative regulation of D-glucose import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902042">
        <property type="term" value="P:negative regulation of extrinsic apoptotic signaling pathway via death domain receptors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902043">
        <property type="term" value="P:positive regulation of extrinsic apoptotic signaling pathway via death domain receptors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd08338">
        <property type="entry name" value="DED_PEA15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.533.10:FF:000026">
        <property type="entry name" value="astrocytic phosphoprotein PEA-15 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001875">
        <property type="entry name" value="DED_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029546">
        <property type="entry name" value="PEA15_DED"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48169:SF1">
        <property type="entry name" value="ASTROCYTIC PHOSPHOPROTEIN PEA-15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48169">
        <property type="entry name" value="DED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01335">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00031">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50168">
        <property type="entry name" value="DED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DED" evidence="9">
        <location>
            <begin position="40"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005003227.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="167" mass="18893" checksum="0ABAD9E54ECC177D" modified="2023-09-13" version="2">MVPGADSQRALFLYQPGRATAGPFCLLAPGCASERAFMAEYGTLLQDLTNNITLEDLEQLKSACKEDIPSEKSEEITTGSAWFSFLESHNKLDKDNLSYIEHIFEISRRPDLLTMVVDYRTRVLKISEEDELDTKLTRIPSAKKYKDIIRQPSEEEIIKLAPPPKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PTV8</accession>
    <name>A0A8C4PTV8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Zinc finger protein 784</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="primary">ZNF784</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="17" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="17" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4PTV8"/>
    <dbReference type="Ensembl" id="ENSEAST00005033084.2">
        <property type="protein sequence ID" value="ENSEASP00005030432.2"/>
        <property type="gene ID" value="ENSEASG00005020727.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162820"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000446">
        <property type="entry name" value="Zinc finger protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="zinc finger protein 784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000787">
        <property type="entry name" value="Zinc finger protein 784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001426">
        <property type="entry name" value="zinc finger protein 784"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379">
        <property type="entry name" value="KRAB AND ZINC FINGER DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24379:SF133">
        <property type="entry name" value="ZFP617 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="106"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="173"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="201"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="16">
        <location>
            <begin position="229"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="127"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="246"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="286"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00069084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030432.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="301" mass="32067" checksum="F42C4A6A2295259B" modified="2023-09-13" version="2">QLQVLVPDDGHPTTPPSDLIEIQVVKVTDTTLVPEPPEPGSLHCALCPAAFRLVSELLFHEHGHLAQAEGGRQGGDPSRCHVCGHSCPGPASLRAHYSLHTGERPYRCALCPRAFKALAPLLRHQHRHGVEPGTSQRPPEAAAARDPRPGVPQERSEVVMAAAAAGAAVGKPFACRFCAKPFRRSSDMRDHERVHTGERPYHCGVCGKGFTQSSVLSGHARIHTGERPFRCALCDRTFNNSSNFRKHQRTHFHGPGPGPGLGDSGSQLASGAEGSGSGCGAGNTLEEGRGETAKVKVEVDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PUF9</accession>
    <name>A0A8C4PUF9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADP-retinol dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.300</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">RDH13</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>all-trans-retinol + NADP(+) = all-trans-retinal + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25033"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17336"/>
            <dbReference type="ChEBI" id="CHEBI:17898"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.300"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; retinol metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.300" evidence="3"/>
    <dbReference type="RefSeq" id="XP_014712125.1">
        <property type="nucleotide sequence ID" value="XM_014856639.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PUF9"/>
    <dbReference type="Ensembl" id="ENSEAST00005033605.2">
        <property type="protein sequence ID" value="ENSEASP00005030906.1"/>
        <property type="gene ID" value="ENSEASG00005021074.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106840917"/>
    <dbReference type="KEGG" id="eai:106840917"/>
    <dbReference type="CTD" id="112724"/>
    <dbReference type="GeneTree" id="ENSGT00940000159641"/>
    <dbReference type="OMA" id="LMCATEP"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052650">
        <property type="term" value="F:all-trans-retinol dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042462">
        <property type="term" value="P:eye photoreceptor cell development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009644">
        <property type="term" value="P:response to high light intensity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010842">
        <property type="term" value="P:retina layer formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042574">
        <property type="term" value="P:retinal metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd09807">
        <property type="entry name" value="retinol-DH_like_SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000145">
        <property type="entry name" value="Retinol dehydrogenase 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS F PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43157:SF59">
        <property type="entry name" value="RETINOL DEHYDROGENASE 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034928689" description="NADP-retinol dehydrogenase" evidence="9">
        <location>
            <begin position="17"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005030906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="334" mass="36279" checksum="7D37FB263A852438" modified="2022-01-19" version="1">MNRYLLPLSVLGTVAGGAVLLRDYVAGGACPSKATIHGKTVIVTGANTGIGKQTALELAKRGGNVILACRDMEKCEAAAKEIRGETLNHHVNARHLDLASLRSVREFATKILAEEEQVHVLVNNAAVMRCPHWTTEDGFEMQFGVNYLGHFLLTNLLLDKLKASAPSRIINLSSLAHVAGHIDFDDLNWEKRKYDTKAAYCQSKLAIVLFTKELSRRLQGTGVTVNALHPGVARTELGRHTGLHSSAFSSFMLGPIFWLLVKSPQLAAQPSTYLAVAEELEGVSGKYFDGLKEKAPAPEAEDEEVARRLWAESAHLVGLEMSHGSSVKGQPLPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PUI0</accession>
    <name>A0A8C4PUI0_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Ephrin A2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">EFNA2</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9 10">Belongs to the ephrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044609641.1">
        <property type="nucleotide sequence ID" value="XM_044753706.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PUI0"/>
    <dbReference type="SMR" id="A0A8C4PUI0"/>
    <dbReference type="Ensembl" id="ENSEAST00005034620.2">
        <property type="protein sequence ID" value="ENSEASP00005031810.1"/>
        <property type="gene ID" value="ENSEASG00005021688.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106834223"/>
    <dbReference type="KEGG" id="eai:106834223"/>
    <dbReference type="CTD" id="1943"/>
    <dbReference type="GeneTree" id="ENSGT00940000160040"/>
    <dbReference type="OMA" id="HMEHYVL"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046875">
        <property type="term" value="F:ephrin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046849">
        <property type="term" value="P:bone remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048013">
        <property type="term" value="P:ephrin receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030316">
        <property type="term" value="P:osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd10425">
        <property type="entry name" value="Ephrin-A_Ectodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.420:FF:000005">
        <property type="entry name" value="Ephrin A5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031328">
        <property type="entry name" value="Ephrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034252">
        <property type="entry name" value="Ephrin-A_Ecto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019765">
        <property type="entry name" value="Ephrin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001799">
        <property type="entry name" value="Ephrin_RBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304">
        <property type="entry name" value="EPHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11304:SF4">
        <property type="entry name" value="EPHRIN-A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00812">
        <property type="entry name" value="Ephrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01347">
        <property type="entry name" value="EPHRIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01299">
        <property type="entry name" value="EPHRIN_RBD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51551">
        <property type="entry name" value="EPHRIN_RBD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="5 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034691304" evidence="11">
        <location>
            <begin position="28"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Ephrin RBD" evidence="12">
        <location>
            <begin position="35"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005031810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="214" mass="23973" checksum="A5BC2E78A3D7342A" modified="2022-01-19" version="1">MAPAQRPLLPLLLLLLPLPPPPPFARAEDAARANSDRYAVYWNRSNPRFHAGAADDGGGYTVEVSINDYLDIYCPHYGAPLPPAERMEHYVLYMVNGEGHASCDHRQRGFKRWECNRPAAPGGPLKFSEKFQLFTPFSLGFEFRPGHEYYYISATPPNAVDRPCLRLKVYVRPTNESLYESPEPIFTSNNSCGAPRGRRLLLGAVPVLWTLLGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4PVT6</accession>
    <name>A0A8C4PVT6_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Zinc finger protein 512B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ZNF512B</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014704550.1">
        <property type="nucleotide sequence ID" value="XM_014849064.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044603763.1">
        <property type="nucleotide sequence ID" value="XM_044747828.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4PVT6"/>
    <dbReference type="Ensembl" id="ENSEAST00005036885.2">
        <property type="protein sequence ID" value="ENSEASP00005033836.2"/>
        <property type="gene ID" value="ENSEASG00005023148.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106836295"/>
    <dbReference type="KEGG" id="eai:106836295"/>
    <dbReference type="CTD" id="57473"/>
    <dbReference type="GeneTree" id="ENSGT00940000159165"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902894">
        <property type="term" value="P:negative regulation of miRNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000177">
        <property type="entry name" value="Zinc finger protein 512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000270">
        <property type="entry name" value="Zinc finger protein 512"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000820">
        <property type="entry name" value="Zinc finger protein 512B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000857">
        <property type="entry name" value="Zinc finger protein 512B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052274">
        <property type="entry name" value="Krueppel_C2H2_Zn-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048408">
        <property type="entry name" value="ZNF512_C2HC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22979:SF3">
        <property type="entry name" value="ZINC FINGER PROTEIN 512B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22979">
        <property type="entry name" value="ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21276">
        <property type="entry name" value="ZNF512_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="141"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="547"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="637"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="13">
        <location>
            <begin position="791"/>
            <end position="815"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="333"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="568"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="653"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="819"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="377"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="425"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="826"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="856"/>
            <end position="882"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005033836.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="898" mass="97756" checksum="270F895EF045CFB9" modified="2023-09-13" version="2">MADPFCVGGGRRLPGSSKSGPGKDGSRSEVRLPVLHDPPKLGVPVVRGGQTVPSQAPLCFDPGSQATDRTEGKKKGRPKAENQALRDIPLSLMNQWKDEFKAHSRVKCPNSGCWLEFPSIYGLKYHYQRCQGGAISERLTFPCPFCEAAFTSKTQLEKHRIWNHMDRPLPTPKPGPVSRPVTISRPVGVSKPIGVSKPVTIGKPVGVSKPIGISKPVTVSRPVPVTKTVTVSRPVPVTKPVTVSRPVPVTKAVPVTKPVTVNKPVPVTKPVPVTKPVTINKPVPMTKLVTVTKPVPVTKLVTVSRPIVVSKPVTVSRPIAISRHTTPCKMVLLTKSENKTPRAAGRSSGKKRAADSLDACPVLPKQARPENGECGPSTASQGSAFQLSTDPSGGPLALGSRPLGGKEVPRAAGPGSPPEEGTERTKHRRKQKTPKKFTGEQPSISGTFGLKGLAKAEDKSRAHRTKKQEGPSPEDVRKKVPAPTSTVSKEVPAPVAHPGPGGPEEQWQRAIHERGEAVCPTCNVVARKTLVGLKKHMEVCQKLQDALKCQHCRKQFKSKAGLNYHTMAEHSTKPSDAEASEGSEQEERERLRKVLKQMGRLRCPQEGCGAAFSSLMGYQYHQRRCGKPPCEVDSPSFPCIHCGKTYRSKAGHDYHMRSEHTAPPPEEAEDKPAEAEDLLGVERTPSGRIRRTSAQVAVFHLQEIAEDELARDWTKRRMKDDLVPETARLNYTRPGLPTVNPQLLEAWKNEVKEKGHVNCPNDCCEAIYSSVSGLKAHLASCSKGDHLVGKYCCLLCPKEFSSESGVKYHILKTHAENWFRTSADPPPKHRSQDSLAPKKEEKSLAGGKKRGRKPKERPPEEPAPKMPPRRDDWPPGGRDKGARGSTARKVGASKPPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0I4K1</accession>
    <name>A0A9L0I4K1_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">BICD family like cargo adaptor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">BICDL1</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0I4K1"/>
    <dbReference type="Ensembl" id="ENSEAST00005062514.1">
        <property type="protein sequence ID" value="ENSEASP00005034898.1"/>
        <property type="gene ID" value="ENSEASG00005007623.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157442"/>
    <dbReference type="OMA" id="PRQFGQY"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055107">
        <property type="term" value="P:Golgi to secretory granule transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051149">
        <property type="entry name" value="Spindly/BICDR_Dynein_Adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123">
        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123:SF12">
        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="68"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="387"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="122"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="249"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="468"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="80"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="388"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005034898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="574" mass="64778" checksum="B145172083FB6EFF" modified="2023-09-13" version="1">MSAFCLGLAGRVSAPAEPDSACCMELPAAAADAVASPAAAAALISFPGGPGELELALEEELALLAAGERPSDPGEHPQAEPGPPAEGAGPQPPPAQDPELLSVIRQKEKDLVLAARLGKALLERNQDMSRQYEQMHKELTDKLEHLEQEKHELRRRFENREGEWEGRVSELESDVKQLQDELERQQVHLREADREKTRAVQELSEQNQRLLDQLSRASEVERQLSMQVHALREDFREKNSSTNQHIIRLESLQAEIKMLSDRKRELEHRLSATLEENDLLQGTVEELQDRVLILERQGHDKDLQLHQSQLELQEVRLSYRQLQGKVEELTEERSLQSSAATSTSLLSEIEQSMEAEELEQEREQLRLQLWEAYCQVRYLCSHLRGNDSADSAVSTDSSMDESSETSSAKDVPAGSLRTALSELKRLIQSIVDGMEPTVTLLSVEMTALKEERDQLRVTSEDKEPKEQLQKAIRDRDEAIAKKNAVELELAKCKMDMMSLNSQLLDAIQQKLNLSQQLEAWQDDMHRVIDRQLMDTHLKERSRPAAALARAHGAGRGDEPSTTEGKRLFSFFRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IAR7</accession>
    <name>A0A9L0IAR7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="25">Protein S100</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="25">S100 calcium-binding protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="28" type="primary">LOC106846279</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="28 29" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
                <person name="J."/>
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                <person name="Zhao"/>
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                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
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                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="28" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="28" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="24">Homodimer. Preferentially exists as a heterodimer or heterotetramer with S100A8 known as calprotectin (S100A8/A9). S100A9 interacts with ATP2A2. S100A9 interacts with AGER, and with the heterodimeric complex formed by TLR4 and LY96 in the presence of calcium and/or zinc ions. S100A9 binds quinoline-3-carboxamides in the presence of calcium and/or zinc ions. S100A9 interacts with amyloid-beta protein 40. Calprotectin (S100A8/9) interacts with CEACAM3 and tubulin filaments in a calcium-dependent manner. Heterotetrameric calprotectin (S100A8/A9) interacts with ANXA6 and associates with tubulin filaments in activated monocytes. Calprotectin (S100A8/9) interacts with NCF2/P67PHOX, RAC1, RAC2, CYBA and CYBB. Calprotectin (S100A8/9) interacts with NOS2 to form the iNOS-S100A8/A9 transnitrosylase complex; induced by LDL(ox). Calprotectin (S100A8/9) interacts with CD69.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 25">Belongs to the S-100 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044614020.1">
        <property type="nucleotide sequence ID" value="XM_044758085.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070353731.1">
        <property type="nucleotide sequence ID" value="XM_070497630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IAR7"/>
    <dbReference type="Ensembl" id="ENSEAST00005055393.1">
        <property type="protein sequence ID" value="ENSEASP00005037914.1"/>
        <property type="gene ID" value="ENSEASG00005028352.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106846279"/>
    <dbReference type="GeneTree" id="ENSGT00940000161606"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005856">
        <property type="term" value="C:cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070062">
        <property type="term" value="C:extracellular exosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016209">
        <property type="term" value="F:antioxidant activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:calcium ion binding"/>
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        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0048306">
        <property type="term" value="F:calcium-dependent protein binding"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:antimicrobial humoral immune response mediated by antimicrobial peptide"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
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        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0014002">
        <property type="term" value="P:astrocyte development"/>
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        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0035425">
        <property type="term" value="P:autocrine signaling"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:autophagy"/>
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        <property type="term" value="P:chronic inflammatory response"/>
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        <property type="term" value="P:endothelial cell migration"/>
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        <property type="term" value="P:innate immune response"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:leukocyte migration involved in inflammatory response"/>
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        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:neutrophil aggregation"/>
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        <property type="term" value="P:neutrophil chemotaxis"/>
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        <property type="term" value="P:regulation of integrin biosynthetic process"/>
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        <property type="entry name" value="calgranulins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EF-hand"/>
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        <property type="entry name" value="EF-hand-dom_pair"/>
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        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
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        <property type="entry name" value="S100/CaBP7/8-like_CS"/>
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        <property type="entry name" value="S100_Ca-bd_sub"/>
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        <property type="entry name" value="PROTEIN S100-A9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11639">
        <property type="entry name" value="S100 CALCIUM-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01023">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01394">
        <property type="entry name" value="S_100"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS00303">
        <property type="entry name" value="S100_CABP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="19" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="13" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="20" id="KW-0072">Autophagy</keyword>
    <keyword evidence="17 25" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="23" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="18" id="KW-0391">Immunity</keyword>
    <keyword evidence="22" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="12" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="21" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 25" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0488">Methylation</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0964">Secreted</keyword>
    <keyword evidence="16" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="EF-hand" evidence="27">
        <location>
            <begin position="53"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="26">
        <location>
            <begin position="96"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="26">
        <location>
            <begin position="117"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="26">
        <location>
            <begin position="132"/>
            <end position="148"/>
        </location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
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    <evidence type="ECO:0000256" key="2">
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            <dbReference type="ARBA" id="ARBA00004245"/>
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            <dbReference type="ARBA" id="ARBA00004613"/>
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            <dbReference type="ARBA" id="ARBA00022862"/>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00046583"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005037914.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="148" mass="16633" checksum="3EA4692AEEC594FD" modified="2023-09-13" version="1">MAELSQMERDLETIINVFHQYSVRLGHPDTLNQKEFKQLVQKELANFLQNEQKDEGAINHILEDLDTNEDKQLSFEEFIMLVIRLTLASHQKMHENAPKLPGHHHGGGLGEGGSQHEGSHEDHGHSHEGHGHSHGGRGHSHRGPGHSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IBJ1</accession>
    <name>A0A9L0IBJ1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphodiesterase</fullName>
            <ecNumber evidence="10">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">PDE4D</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3',5'-cyclic AMP + H2O = AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58165"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.4.53"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:25278"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10">Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="10"/>
    <dbReference type="RefSeq" id="XP_044627902.1">
        <property type="nucleotide sequence ID" value="XM_044771967.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IBJ1"/>
    <dbReference type="Ensembl" id="ENSEAST00005049826.1">
        <property type="protein sequence ID" value="ENSEASP00005038237.1"/>
        <property type="gene ID" value="ENSEASG00005011890.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106843380"/>
    <dbReference type="CTD" id="5144"/>
    <dbReference type="GeneTree" id="ENSGT00940000155674"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004115">
        <property type="term" value="F:3',5'-cyclic-AMP phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1300.10:FF:000001">
        <property type="entry name" value="Phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1300.10">
        <property type="entry name" value="3'5'-cyclic nucleotide phosphodiesterase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040844">
        <property type="entry name" value="PDE4_UCR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023088">
        <property type="entry name" value="PDEase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002073">
        <property type="entry name" value="PDEase_catalytic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036971">
        <property type="entry name" value="PDEase_catalytic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023174">
        <property type="entry name" value="PDEase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11347">
        <property type="entry name" value="CYCLIC NUCLEOTIDE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18100">
        <property type="entry name" value="PDE4_UCR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00233">
        <property type="entry name" value="PDEase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00387">
        <property type="entry name" value="PDIESTERASE1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00126">
        <property type="entry name" value="PDEASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51845">
        <property type="entry name" value="PDEASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0114">cAMP</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PDEase" evidence="12">
        <location>
            <begin position="323"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="40"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="647"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="699"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="716"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="399"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="399"/>
            <end position="403"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="403"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
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        <location>
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        <ligand>
            <name>AMP</name>
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        <location>
            <position position="440"/>
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        <ligand>
            <name>Zn(2+)</name>
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            <label>1</label>
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        <location>
            <position position="440"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
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        <location>
            <position position="557"/>
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        <ligand>
            <name>AMP</name>
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        <location>
            <position position="557"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
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        <location>
            <position position="608"/>
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        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009517"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023149"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00033681"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-1"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-2"/>
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    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR623088-3"/>
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    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51845"/>
        </source>
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    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="746" mass="84573" checksum="D2B2638A0245281F" modified="2023-09-13" version="1">MAQQTTSPDTLTVPEVDNPRCPNAWLNEDLVKSLRENLLQHEKSKTSRKSVSPKLSPVISPRNSPRLLRRMLLSSNIPKQRRFTVAHTCFDVDNGTSSGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSSIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDQEEGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVGEEEESQPEACAVEDRSPDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IBX7</accession>
    <name>A0A9L0IBX7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="34">NACHT, LRR and PYD domains-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="40" type="primary">NLRP3</name>
    </gene>
    <organism evidence="40 41">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="40 41" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="40" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="40" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="35">Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth. During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription. Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3'. May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="36">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="36">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Microtubule organizing center</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Golgi apparatus membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Inflammasome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the NLRP family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IBX7"/>
    <dbReference type="Ensembl" id="ENSEAST00005082711.1">
        <property type="protein sequence ID" value="ENSEASP00005038387.1"/>
        <property type="gene ID" value="ENSEASG00005017967.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000162415"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072559">
        <property type="term" value="C:NLRP3 inflammasome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140297">
        <property type="term" value="F:DNA-binding transcription factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006954">
        <property type="term" value="P:inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002674">
        <property type="term" value="P:negative regulation of acute inflammatory response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901223">
        <property type="term" value="P:negative regulation of non-canonical NF-kappaB signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00116">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08320">
        <property type="entry name" value="Pyrin_NALPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.10.10:FF:000360">
        <property type="entry name" value="NACHT, LRR and PYD domains-containing protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.533.10">
        <property type="entry name" value="Death Domain, Fas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004020">
        <property type="entry name" value="DAPIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011029">
        <property type="entry name" value="DEATH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029495">
        <property type="entry name" value="NACHT-assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007111">
        <property type="entry name" value="NACHT_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041267">
        <property type="entry name" value="NLRP_HD2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050637">
        <property type="entry name" value="NLRP_innate_immun_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041075">
        <property type="entry name" value="NOD1/2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45690">
        <property type="entry name" value="NACHT, LRR AND PYD DOMAINS-CONTAINING PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45690:SF19">
        <property type="entry name" value="NACHT, LRR AND PYD DOMAINS-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14484">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13516">
        <property type="entry name" value="LRR_6"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05729">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17776">
        <property type="entry name" value="NLRC4_HD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02758">
        <property type="entry name" value="PYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17779">
        <property type="entry name" value="WHD_NOD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01288">
        <property type="entry name" value="FISNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SMART" id="SM01289">
        <property type="entry name" value="PYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47986">
        <property type="entry name" value="DEATH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50824">
        <property type="entry name" value="DAPIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50837">
        <property type="entry name" value="NACHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="27" id="KW-0010">Activator</keyword>
    <keyword evidence="19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="30" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="18" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="23" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="17" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="21" id="KW-0391">Immunity</keyword>
    <keyword evidence="31" id="KW-1271">Inflammasome</keyword>
    <keyword evidence="29" id="KW-0395">Inflammatory response</keyword>
    <keyword evidence="13" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="10" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="14" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="33" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="25" id="KW-0472">Membrane</keyword>
    <keyword evidence="24" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="32" id="KW-0539">Nucleus</keyword>
    <keyword evidence="26" id="KW-0564">Palmitate</keyword>
    <keyword evidence="12" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="41" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0964">Secreted</keyword>
    <keyword evidence="28" id="KW-0804">Transcription</keyword>
    <keyword evidence="22" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="20" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Pyrin" evidence="38">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="NACHT" evidence="39">
        <location>
            <begin position="213"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="37">
        <location>
            <begin position="681"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="37">
        <location>
            <begin position="684"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00008665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00022859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00023198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00023233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="33">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="34">
        <source>
            <dbReference type="ARBA" id="ARBA00040040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="35">
        <source>
            <dbReference type="ARBA" id="ARBA00045987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="36">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="37">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="38">
        <source>
            <dbReference type="PROSITE" id="PS50824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="39">
        <source>
            <dbReference type="PROSITE" id="PS50837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="40">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005038387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="41">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1000" mass="114064" checksum="07DA1DE39A014641" modified="2023-09-13" version="1">MASVRCKLARYLEDLEDADLKKFKMHLEDYPPQKGCTRPPRGQTEKADPVDLATLMIDCNGEEKAWAMAVWIFAAINRRDLHEKAKLEEPEWDHSHLICQEESIEEEWIGLLEYLSRISICKKKKDYCKKYRKHVRSRFQCIKDRNARLGESVNLNKRYTRLCLLKEHRSQQEREQELLAIGRTSPKTQDCPMSSMNLELLFEPDDQHSEPVHTVVFQGAAGIGKTILARKIMLDWASEKLYKNRFDYLFYIHCREVSLGTRRSLGDLIVSCCPDPKPPIGKILSKPSRILFLMDGFDELQGAFDEHTEALCTDWQKVERGDILLSSLIRKRLLPEASLLITTRPVALEKLQHLLDRPRHVEILGFSEAKRKEYFFKYFSDEQQAREAFRLIQENEILFTMCFIPLVCWIVCTGLKQQMDSGKSLAQTSKTTTAVYVFFLSSLLQSRGGSQKQHLSANVQGLCSLAADGIWNQKILFEESDLRKHGLQRADASAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEDEEQGSVRNVPWSRSNLPNRDVSVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWISVKAEVKKLQTQPSQLELFYCLYEMQEEDFVRRAMGYFPKIEISLSTRMDHVVSSFCIENCRSVESLSLRLLYNSPKEEEEEEEEEEEDGVRHSDMDRCVPSDATYSPRLVNCSLTSSFCQSLFSALSTNWSLTELNLSDNTLGDSGMKVLCDMLQHPGCNIQRLWLGRCWLSHQCCFNISSVLSSNQKLVELDLSHNALGDFGIRLLCVGLRHLFCNLKTLWLVGCCLTSACCEDLASVLSTSHSLIRLYLGENALGDSGVGILCEKAKHPRCNLQVLGLVNSGLTSGCCPALSSVLSANHNLKQLYLRGNALGDTGVKLLCEGLSHPNCKLQMLELDSCSLTSHCCWDLSTLLTSNQSLRELSLGGNDLGDLGVMLLCEVLKQQGCLLRSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0ICA3</accession>
    <name>A0A9L0ICA3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 19">tRNA dimethylallyltransferase</fullName>
            <ecNumber evidence="4 19">2.5.1.75</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="primary">TRIT1</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="23" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="23" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="18">Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 of both cytosolic and mitochondrial tRNAs, leading to the formation of N6-(dimethylallyl)adenosine (i6A37). Mediates modification of a limited subset of tRNAs: tRNA(Ser)(AGA), tRNA(Ser)(CGA), tRNA(Ser)(UGA), as well as partial modification of the selenocysteine tRNA(Ser)(UCA). TRIT1 is therefore required for selenoprotein expression.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 19">
            <text>adenosine(37) in tRNA + dimethylallyl diphosphate = N(6)-dimethylallyladenosine(37) in tRNA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:26482"/>
            <dbReference type="Rhea" id="RHEA-COMP:10162"/>
            <dbReference type="Rhea" id="RHEA-COMP:10375"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57623"/>
            <dbReference type="ChEBI" id="CHEBI:74411"/>
            <dbReference type="ChEBI" id="CHEBI:74415"/>
            <dbReference type="EC" id="2.5.1.75"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19 20">Belongs to the IPP transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.75" evidence="4 19"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0ICA3"/>
    <dbReference type="Ensembl" id="ENSEAST00005072203.1">
        <property type="protein sequence ID" value="ENSEASP00005038507.1"/>
        <property type="gene ID" value="ENSEASG00005000884.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000015214"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052381">
        <property type="term" value="F:tRNA dimethylallyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070900">
        <property type="term" value="P:mitochondrial tRNA modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd02019">
        <property type="entry name" value="NK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001625">
        <property type="entry name" value="tRNA dimethylallyltransferase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.140:FF:000002">
        <property type="entry name" value="tRNA dimethylallyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00185">
        <property type="entry name" value="IPP_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039657">
        <property type="entry name" value="Dimethylallyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030666">
        <property type="entry name" value="IPP_transferase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018022">
        <property type="entry name" value="IPT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00174">
        <property type="entry name" value="miaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11088:SF89">
        <property type="entry name" value="TRNA DIMETHYLALLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01715">
        <property type="entry name" value="IPPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039110">
        <property type="entry name" value="IPP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 19 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="11 19 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 19 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="15" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9 19" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="13" id="KW-0862">Zinc</keyword>
    <keyword evidence="12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger double-stranded RNA binding" evidence="22">
        <location>
            <begin position="395"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="422"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
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            <dbReference type="ARBA" id="ARBA00057810"/>
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            <dbReference type="Pfam" id="PF12171"/>
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    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="467" mass="52578" checksum="F09DC775EFF3F7A2" modified="2023-09-13" version="1">AAAAAAARAVSVGPGLRGLQGTLPLVVILGATGTGKSTLALQLGQRLGGEIVSADSMQVYEGLDIITNKVSAREQRMCQHHMISFVDPLVTNYTVVDFRNKATALIEDIFARDKIPIVVGGTNYYIEALLWKVLVNAKPQEMDTEKVIDRKVELEKEDGHVLHKRLSQVDPERAAKLHPHDKRKVARSLQVFEETGISHSEFLHRQQAEEGGGPLGGPLKFPNPCILWLHTDQTVLDERLDKRVDDMLAAGLLDELRDFHRRYNQKKVAENSQDYQHGIFQSIGFKEFHEYLITEGKCTPETSNQLLKKGIEALKQVTKRYARKQNRWVKNRFLSRPGPSVPPVYGLEVSDVSKWEESVLEPALEIVRSFIQGHQPAAAPVKMPCNETENKRSYHMCDLCDRIIIGDREWAAHRKSKSHLHQLKKRRKLDSDAVATIESQSISPDRDKELKEKGSPGQNDEELKFSV</sequence>
</entry>
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            <fullName evidence="2">Leucine-rich repeat-containing protein 14</fullName>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
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                <person name="Zhuang"/>
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                <person name="Li"/>
                <person name="R."/>
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                <person name="M."/>
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                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Xu"/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="5" key="2">
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="5" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
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        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the PRAME family. LRRC14 subfamily.</text>
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                <person name="Jin"/>
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                <person name="G."/>
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                <person name="J."/>
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                <person name="Jordana"/>
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                <person name="Noce"/>
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                <person name="Wu"/>
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                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    </dbReference>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005039447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="126" mass="14361" checksum="E6C06ED7A585E3C1" modified="2023-09-13" version="1">MDEDFSPQVKKMALAIGTSLLDKDKLLPTDTRDQGSFNYLKFSEYMKEFQAMGQLESVIPKAFQTLDKDKSCFIKWNEIKYILSIASSGPAALLMAEKAEAIIQVADRIDSEEFFELIKKKIPKEK</sequence>
</entry>
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    <accession>A0A9L0IH33</accession>
    <name>A0A9L0IH33_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Achaete-scute family bHLH transcription factor 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">ASCL2</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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        <lineage>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
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            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
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    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="J."/>
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                <person name="Zhao"/>
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                <person name="Wang"/>
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                <person name="Liu"/>
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                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
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                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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                <person name="Zhang"/>
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                <person name="Liu"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
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                <person name="Yu"/>
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                <person name="Yin"/>
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                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
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                <person name="Yue"/>
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                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
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                <person name="J."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
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        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 53"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR24160:SF1">
        <property type="entry name" value="ANKYRIN REPEAT DOMAIN-CONTAINING PROTEIN 53"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="211"/>
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        </location>
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    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="248"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="140"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
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        <location>
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            <fullName evidence="3">Family with sequence similarity 133 member B</fullName>
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    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Belongs to the FAM133 family.</text>
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        <property type="gene ID" value="ENSEASG00005020075.2"/>
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    <dbReference type="KEGG" id="eai:106832258"/>
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    <dbReference type="GeneTree" id="ENSGT00730000111097"/>
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        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026766">
        <property type="entry name" value="Fam133"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31911">
        <property type="entry name" value="PROTEIN FAM133"/>
        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR31911:SF3">
        <property type="entry name" value="PROTEIN FAM133B"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <begin position="19"/>
            <end position="38"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="70"/>
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            <begin position="70"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="2">
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            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005041415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="247" mass="28379" checksum="172284D295D0D414" modified="2023-09-13" version="1">MGKRDNRVAYMNPIAMARSRGPIQSSGPTIQDYLNRPRPTWEEVKEQLEKKKKGSKALAEFEEKMNENWKKELEKHREKLLSGSESSSKKRQRKKKEKKKSGRYSSSSSSSSDSSSSSSDSEDEDKKQGKRKKKKKNRSHKSSESSISETESDSKDSLKKKKKSKDATEKEKDIKGLSKKRKMYPEDKPLSSESLSESDYIEEVRAKKKKSSEEREKTTEKTKKKKKHKKHSKKKKKKAASSSPDSP</sequence>
</entry>
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        <recommendedName>
            <fullName evidence="13">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
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        <name type="scientific">Equus asinus</name>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="J."/>
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                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="1">Putative odorant or sperm cell receptor.</text>
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            <topology evidence="2">Multi-pass membrane protein</topology>
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        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0807">Transducer</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="98"/>
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    <feature type="transmembrane region" description="Helical" evidence="12">
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    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="197"/>
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            <begin position="41"/>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005043089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="313" mass="35259" checksum="B3A04FF5B51DC675" modified="2023-09-13" version="1">IAKGNHSQVSEFILLGLTDNPKLQVILFGVFLGIYLVSVMGNLGLIVLIQISPQLHTPMYFFLSHLAFIDFSFTSSVTPNTLVHFLCDIKSITFYGCAIQVCCFITFVVCELYLLAIMAYDWYVAICSPLLYVTLMPRKLCYRIIASTYVYGFAVGLGQTVATFRLSFCGSNMVNHFYCDDVPLVALACSDTHIKELMLLIIAGFNTLCSLLNVMISYVFILFAVLRIHSAEGRQKAFSTCASHLTSITIFYGTIIFMYVLHNSRHSLNTDKFASEFYVVVIPMLNPLIYSLRNQEVKNALKRIREKLCLAIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IS25</accession>
    <name>A0A9L0IS25_EQUAS</name>
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        <submittedName>
            <fullName evidence="5">Glutamine amidotransferase class 1 domain containing 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">GATD3</name>
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    <organism evidence="5 6">
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    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
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                <person name="Li"/>
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                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
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                <person name="Wang"/>
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                <person name="Zhuang"/>
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                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
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                <person name="Li"/>
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                <person name="Li"/>
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                <person name="Hou"/>
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                <person name="Liu"/>
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                <person name="Jin"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="ES1 protein homolog, mitochondrial isoform X1"/>
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        <property type="entry name" value="Class_I_gatase-like"/>
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        <property type="entry name" value="ES1 PROTEIN HOMOLOG, MITOCHONDRIAL"/>
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        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE-LIKE CLASS 1 DOMAIN-CONTAINING PROTEIN 3, MITOCHONDRIAL-RELATED"/>
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        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
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        <location>
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        <source>
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        <source>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005043776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
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    </evidence>
    <sequence length="373" mass="39346" checksum="6CA75CB581DCCFD1" modified="2023-09-13" version="1">MLSRCCPAPGPETRDTGPGQRSREPDTRSGREAAGRANPSARGRDHPLPRGQSQSSPGRLRRRGPAPGALAGVERDEVTAQGRPAGFRAWLRGHVTLRPPPSRAPMAAVRALMAPRLAAASALAPLPGNTRMPSPRAALHGSAPRPGPKVALVLSGCGVYDGTELHEASAILVHLSRGGAEVQIFAPDVPQMHVIDHTKGQPAESETRNVLTESARIARGKITDLARLSAANHDAAIFPGGFGAAKNLSTFAVDGKDCKVNKDVERVLKEFHEAGKPIGLCCIAPVLAARVLRGVEVTVGHEQEEGGKWPYAGTAEAIKALGAKHCVKGVTEAHVDQKNKVVTTPAFMCETALHHIHDGIGAMVKKVLELTRK</sequence>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING"/>
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        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING PROTEIN 1"/>
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        <location>
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            <end position="81"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005043583.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="8812" checksum="B474ADE473CD3F20" modified="2023-09-13" version="1">MEAVRLPSTAGKFVVVGGGIAGVTCAEQLAIHFPSEDILLVTASPVIKAVTNFKQFKKPVRKAEDEGKQGLHNKERVLRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IV29</accession>
    <name>A0A9L0IV29_EQUAS</name>
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        <submittedName>
            <fullName evidence="11">CCAAT enhancer binding protein alpha</fullName>
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    <gene>
        <name evidence="11" type="primary">CEBPA</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="D.D."/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2">Belongs to the bZIP family. C/EBP subfamily.</text>
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        <property type="protein sequence ID" value="ENSEASP00005044739.1"/>
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    <dbReference type="KEGG" id="eai:106827744"/>
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    <dbReference type="OMA" id="QMPHLQY"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 26"/>
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    <dbReference type="GO" id="GO:1990647">
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:chromatin DNA binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
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                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="8" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0IVB8"/>
    <dbReference type="Ensembl" id="ENSEAST00005081886.1">
        <property type="protein sequence ID" value="ENSEASP00005041716.1"/>
        <property type="gene ID" value="ENSEASG00005037380.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00950000183029"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005853">
        <property type="term" value="C:eukaryotic translation elongation factor 1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0045202">
        <property type="term" value="C:synapse"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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    <dbReference type="GO" id="GO:0043065">
        <property type="term" value="P:positive regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000090">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_TR_CS"/>
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    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
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    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
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    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="7">
        <location>
            <begin position="5"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
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    </evidence>
    <evidence type="ECO:0000256" key="4">
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        <source>
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        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005041716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="392" mass="42815" checksum="4ABA405AC78A96BB" modified="2023-09-13" version="1">MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETTKYYITIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPAYSEKRYDEIVKEVSAYIKKIGYNPATVPFVPISGWHGDNMLEPSPNMPWFKGWKVERKEGNASGVSLLEALPGDNVGFNVKNVSVKDIRRGNVCGDSKSDPPQEAAQFTSQVIILNHPGQISAGYSPVIDCHTAHIACKFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVEMVPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKNVEKKSGGAGKVTKSAQKAQKAGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
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    <name>A0A9L0IXW7_EQUAS</name>
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        <submittedName>
            <fullName evidence="14">Discoidin, CUB and LCCL domain containing 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">DCBLD1</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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    <dbReference type="Ensembl" id="ENSEAST00005075692.1">
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        <property type="gene ID" value="ENSEASG00005009128.2"/>
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        <property type="component" value="Chromosome 24"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00041">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00057">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000002">
        <property type="entry name" value="Coagulation factor VIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.130.20">
        <property type="entry name" value="LCCL-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.290">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000859">
        <property type="entry name" value="CUB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004043">
        <property type="entry name" value="LCCL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036609">
        <property type="entry name" value="LCCL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050633">
        <property type="entry name" value="Neuropilin_MCO_CoagFactor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035914">
        <property type="entry name" value="Sperma_CUB_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806:SF1">
        <property type="entry name" value="DISCOIDIN, CUB AND LCCL DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46806">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00431">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00754">
        <property type="entry name" value="F5_F8_type_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03815">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00042">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00231">
        <property type="entry name" value="FA58C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00603">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69848">
        <property type="entry name" value="LCCL domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49854">
        <property type="entry name" value="Spermadhesin, CUB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01180">
        <property type="entry name" value="CUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50820">
        <property type="entry name" value="LCCL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040429577" evidence="10">
        <location>
            <begin position="35"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="458"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="domain" description="CUB" evidence="11">
        <location>
            <begin position="41"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="LCCL" evidence="13">
        <location>
            <begin position="152"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="F5/8 type C" evidence="12">
        <location>
            <begin position="260"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="560"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="620"/>
            <end position="699"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="564"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="632"/>
            <end position="649"/>
        </location>
    </feature>
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            <begin position="41"/>
            <end position="68"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004479"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00059"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005045729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="713" mass="77522" checksum="68DE7AB4E6F5987A" modified="2023-09-13" version="1">MGAGACGGRPLAGAARYGLLALLLVVSAPLGLQAAEPGDGCGHFVTYQDSGTMTSKNYPGTYPNHTVCEKTISVPKGKRLILRLGDLDIESQTCASDYLLFTSSSHQYGPYCGSMTVPKELLLNTSEVTVRFESGSHISGRGFLLTYASSDHPDLITCLERANHYMKTEYSKFCPAGCRDIAGDISGNVVDGYRDTSLLCKAAIHAGIIADELGGQISVHQRKGLSRYEGILANGVLSRDGSLSDKRFLFTSNGHIVPSCTRSLSLEPDRQIRASSSWQWVNETGDQVHWSPGQARLQDQGPSWASADSLKNHKQREWLEIDLGEKKKITGIRTAGSTQSNFNFYVKSFVMSFKNNNSKWRTYKGIVNNGEKVFQGNSNFRDPVRNNFIPPIVARYVRVVPQTWHQRIALKVELIGCQITQGNDSMVWRKTSSDTVGSTKGEAIPSEEIPKGINVTTVALPLALLALLVAGMGLFAALRKRKKKGSPYGSATAQKTDCWKQIKYPFARHQSAEFTISYDNEKEMTQKLDLITSDMADYQQPLMIGTGTVTRKGSTFRPMDTEAGEAGPSAEAGSHYDRPLPAGRHEYALPLTNPEPEYATPIVERHLARAHTFSAQSGYRVPGLRPAHKHSLSSGSFSPAAGAGATSAGYQMPQSPAPADPGYDKPKASGCVTADRADANYQKPQLHPTTGDAYSTPRDCLRPLNQTAMTALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IY18</accession>
    <name>A0A9L0IY18_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">LOC106836866</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cell membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014705589.1">
        <property type="nucleotide sequence ID" value="XM_014850103.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IY18"/>
    <dbReference type="Ensembl" id="ENSEAST00005066425.1">
        <property type="protein sequence ID" value="ENSEASP00005042657.1"/>
        <property type="gene ID" value="ENSEASG00005024483.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106836866"/>
    <dbReference type="KEGG" id="eai:106836866"/>
    <dbReference type="GeneTree" id="ENSGT00940000162388"/>
    <dbReference type="OMA" id="VMSSQFC"/>
    <dbReference type="OrthoDB" id="5964498at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48001">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00237">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 10" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0552">Olfaction</keyword>
    <keyword evidence="8 10" id="KW-0675">Receptor</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="9 10" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="192"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="237"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="12">
        <location>
            <begin position="41"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005042657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="311" mass="34421" checksum="2B8592D145064776" modified="2023-09-13" version="1">MALRNHSIITEFILIGLSDDPHIEALLFVLFLGIYILTIMGNLTMLLVVRTDSHLHTPMYFFLSNLSFPDLCFSSVTVPKLLKDLLSEKKTISVEGCLTQVFFVFITAGTEAFLLSVMAYDRYVAICHPLLYGQLMSNQLCVKLVLASWGLASLSSVVIVLLAINLDFCEAQTVQHYTCELPSLFPLSCSDISISIDILICSILLHGFGTFLPVFFSYTRIVSTILSISSTTGRSKAFSTCSSHLIAVILFVGSGLIRYLMPTSGSSLDLLSSLQYSAVTPMLNPLIYSLKNMEVKAAVKRTLGKYLQYFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0IY35</accession>
    <name>A0A9L0IY35_EQUAS</name>
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        <recommendedName>
            <fullName evidence="5">Transmembrane protein 98</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">TMEM98</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Functions as a negative regulator of MYRF in oligodendrocyte differentiation and myelination. Interacts with the C-terminal of MYRF inhibiting MYRF self-cleavage and N-fragment nuclear translocation. The secreted form promotes differentiation of T helper 1 cells (Th1).</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts (via N-terminal region) with MYRF; the interaction inhibits MYRF self-cleavage.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Single-pass type II membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
            <location evidence="2">Extracellular exosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TMEM98 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014717674.1">
        <property type="nucleotide sequence ID" value="XM_014862188.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0IY35"/>
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    <sequence length="226" mass="24597" checksum="5F4FC333B765899D" modified="2023-09-13" version="1">METVVIVAIGVLATIFLASFAALVVVCRQRYCRPRDLLQRYDSKPIVDLIGAMETQSEPSELELDDVVITNPHIEAILENEDWIEDASGLMSHCIAILKICHTLTEKLVAMTMGSGAKMKTSASVSDIIVVAKRISPRVDDVVKSMYPPLDPKLLDARTTALLLSVSHLVLVTRNACHLTGGLDWIDQSLSAAEEHLEVLREAALASEPDKGLPGPEGFLQEQSAI</sequence>
</entry>
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    <accession>A0A9L0IYN6</accession>
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        <recommendedName>
            <fullName evidence="4">Olduvai domain-containing protein</fullName>
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        <name type="scientific">Equus asinus</name>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
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                <person name="Yang"/>
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                <person name="A."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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</entry>
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    <accession>A0A9L0J014</accession>
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                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a hydroperoxide + [thioredoxin]-dithiol = an alcohol + [thioredoxin]-disulfide + H2O</text>
            <dbReference type="Rhea" id="RHEA:62620"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30879"/>
            <dbReference type="ChEBI" id="CHEBI:35924"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.11.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peroxiredoxin family. AhpC/Prx1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.24" evidence="2"/>
    <dbReference type="RefSeq" id="XP_014723371.1">
        <property type="nucleotide sequence ID" value="XM_014867885.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J014"/>
    <dbReference type="Ensembl" id="ENSEAST00005081766.1">
        <property type="protein sequence ID" value="ENSEASP00005043362.1"/>
        <property type="gene ID" value="ENSEASG00005004804.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106848141"/>
    <dbReference type="CTD" id="10549"/>
    <dbReference type="GeneTree" id="ENSGT00940000153430"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome X"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008379">
        <property type="term" value="F:thioredoxin peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033554">
        <property type="term" value="P:cellular response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03015">
        <property type="entry name" value="PRX_Typ2cys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.30.10:FF:000003">
        <property type="entry name" value="Peroxiredoxin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000866">
        <property type="entry name" value="AhpC/TSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050217">
        <property type="entry name" value="Peroxiredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019479">
        <property type="entry name" value="Peroxiredoxin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681:SF171">
        <property type="entry name" value="PEROXIREDOXIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10681">
        <property type="entry name" value="THIOREDOXIN PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10417">
        <property type="entry name" value="1-cysPrx_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00578">
        <property type="entry name" value="AhpC-TSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0049">Antioxidant</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="3" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="7" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040508594" description="thioredoxin-dependent peroxiredoxin" evidence="9">
        <location>
            <begin position="39"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="10">
        <location>
            <begin position="80"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005043362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="272" mass="30522" checksum="8376D8009F8A3E64" modified="2023-09-13" version="1">MEAPRPLPAAFIPAPGSSRRLLLLPLLLLLLPAGPLRGWEAEERPRTREEECHFYAGGQVYPGEASRVSVADHSLHLSKAKISKPAPYWEGTAVINGEFKELKLTDYRGKYLVFFFYPLDFTFVCPTEIIAFGDRIEEFRSINTEVVACSVDSQFTHLAWINTPRRQGGLGPIRIPLLSDLTHQISKDYGVYLEDSGHTLRGLFIIDDKGILRQITLNDLPVGRSVDETLRLVQAFQYTDKHGEVCPAGWKPGSETIIPDPAGKLKYFDKLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J245</accession>
    <name>A0A9L0J245_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bactericidal permeability-increasing protein</fullName>
            <shortName evidence="7">BPI</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">BPIFC</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Monomer. Homodimer; disulfide-linked.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">The N- and C-terminal barrels adopt an identical fold despite having only 13% of conserved residues.</text>
    </comment>
    <comment type="domain">
        <text evidence="7">The N-terminal region may be exposed to the interior of the granule, whereas the C-terminal portion may be embedded in the membrane. During phagocytosis and degranulation, proteases may be released and activated and cleave BPI at the junction of the N- and C-terminal portions of the molecule, providing controlled release of the N-terminal antibacterial fragment when bacteria are ingested.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014707841.2">
        <property type="nucleotide sequence ID" value="XM_014852355.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0J245"/>
    <dbReference type="Ensembl" id="ENSEAST00005059001.1">
        <property type="protein sequence ID" value="ENSEASP00005047336.1"/>
        <property type="gene ID" value="ENSEASG00005019055.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106838342"/>
    <dbReference type="KEGG" id="eai:106838342"/>
    <dbReference type="CTD" id="254240"/>
    <dbReference type="GeneTree" id="ENSGT01150000286994"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050829">
        <property type="term" value="P:defense response to Gram-negative bacterium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045087">
        <property type="term" value="P:innate immune response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.15.20.10:FF:000001">
        <property type="entry name" value="Phospholipid transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.15.10.10:FF:000001">
        <property type="entry name" value="phospholipid transfer protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.10.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.15.20.10">
        <property type="entry name" value="Bactericidal permeability-increasing protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017943">
        <property type="entry name" value="Bactericidal_perm-incr_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030675">
        <property type="entry name" value="BPI/LBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032942">
        <property type="entry name" value="BPI/LBP/Plunc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001124">
        <property type="entry name" value="Lipid-bd_serum_glycop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017942">
        <property type="entry name" value="Lipid-bd_serum_glycop_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10504">
        <property type="entry name" value="BACTERICIDAL PERMEABILITY-INCREASING BPI PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10504:SF17">
        <property type="entry name" value="BPI FOLD-CONTAINING FAMILY C PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01273">
        <property type="entry name" value="LBP_BPI_CETP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02886">
        <property type="entry name" value="LBP_BPI_CETP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002417">
        <property type="entry name" value="Lipid_binding_protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00328">
        <property type="entry name" value="BPI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00329">
        <property type="entry name" value="BPI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55394">
        <property type="entry name" value="Bactericidal permeability-increasing protein, BPI"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0044">Antibiotic</keyword>
    <keyword evidence="7" id="KW-0929">Antimicrobial</keyword>
    <keyword evidence="4 6 7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0391">Immunity</keyword>
    <keyword evidence="7" id="KW-0399">Innate immunity</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040217241" description="Bactericidal permeability-increasing protein" evidence="8">
        <location>
            <begin position="24"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Lipid-binding serum glycoprotein N-terminal" evidence="9">
        <location>
            <begin position="33"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="Lipid-binding serum glycoprotein C-terminal" evidence="10">
        <location>
            <begin position="272"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="6">
        <location>
            <begin position="161"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR002417-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU369039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005047336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="507" mass="56117" checksum="6DFBA61FD3FAAD99" modified="2023-09-13" version="1">MHEKTVHVLWGCFLLWNLHIASSQTIHPGIKARATQRALDYGVQAGMEIIEQMVKERKIPDLKGNESLEFLKIDYVNYNFSNIKINAFSFPNTSLAFVPGVGIKVLTNHGTTNISTDWEVKSPLFQDTGGADLFLSGVYFTGVLILARNDFGQPTVKLQDCYAQVSHAQVSFSGELKVLYNSFAEPMEKPILRNLNEILCPIITSEVEALNANLSVLEVLTKIDNYTRLDYSLISSPEITENYLDLNLKGVFYPLENLTDPPFSPVPIVLPERSDSMLYIGLSKYFFKSASFAYFTAGAFNVTLSTKEISSHLIPNSQGLGSMLSRLAEIYILSQPLMVRIMATEPPVISLLPGNFTLDIPASIIILTQAENSTAETIVSMDFVASTSVGLVILGQRLICSLSLNRFRLSLPESSRGSIEVLRFENILSSIIHFGVLPLANAKLQQGFPLPHPHKISFVNSDVEVLEDLLLISTNLKYETSLKQPPSFPGWADLNLISRQLSGKPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J2E1</accession>
    <name>A0A9L0J2E1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Polyadenylate-binding protein-interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">PAIP1</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs. Its stimulatory activity on translation is mediated via its action on PABPC1. Competes with PAIP2 for binding to PABPC1. Its association with EIF4A and PABPC1 may potentiate contacts between mRNA termini. May also be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with the RRM1-RRM2 and C-terminus regions of PABPC1 in a 1:1 stoichiometry. Interacts with EIF4A.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J2E1"/>
    <dbReference type="Ensembl" id="ENSEAST00005048869.1">
        <property type="protein sequence ID" value="ENSEASP00005046538.1"/>
        <property type="gene ID" value="ENSEASG00005013479.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000153432"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 10"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106002">
        <property type="term" value="C:mCRD-mediated mRNA stability complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008494">
        <property type="term" value="F:translation activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070934">
        <property type="term" value="P:CRD-mediated mRNA stabilization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044794">
        <property type="term" value="P:host-mediated activation of viral process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900152">
        <property type="term" value="P:negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000767">
        <property type="term" value="P:positive regulation of cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006446">
        <property type="term" value="P:regulation of translational initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.180:FF:000016">
        <property type="entry name" value="polyadenylate-binding protein-interacting protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003890">
        <property type="entry name" value="MIF4G-like_typ-3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051367">
        <property type="entry name" value="mRNA_TranslReg/HistoneTransl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009818">
        <property type="entry name" value="PAM2_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23254">
        <property type="entry name" value="EIF4G DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23254:SF15">
        <property type="entry name" value="POLYADENYLATE-BINDING PROTEIN-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02854">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07145">
        <property type="entry name" value="PAM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00543">
        <property type="entry name" value="MIF4G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="MIF4G" evidence="8">
        <location>
            <begin position="160"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="436"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="7">
        <location>
            <begin position="11"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="44"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00053366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00063561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00074029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005046538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="480" mass="53588" checksum="CC938D967DEFDC9C" modified="2023-09-13" version="1">MSDGFDRAPGAGRGRSRGLGRGGGGPEGGGFPNGAGPAERPRHPPPPQPKAPGFLQPPPLRQPRTAPPPGAQCEVPAGPQRPPRPGALPEQARLLRAPPISQDTVPQQNSESAMAKPQVVVAPVLMSKLSVNAPEFYPSGYSSNYAESYEDGCEDYPTLSEYVQDFLNHLTEQPGSFETEIEQFAETLNGWVTTDDALQELVELIYQQATSIPNFSYMGARLCNYLSHHLTISPQSGNFRQLLLKRCRTEYEVKDQAAKGDEVTRKRFHAFVLFLGELYLNLEIKGTNGQVTRADILQVGLRELLNALFSNPMDDNLICAVKLLKLTGSVLEDAWKEKGKTDMEEIIQRIENVVLDANCSRDVKQMLLKLVELRSSNWGRVHATSTYREATPENDPNYFMNEPTFYTSDGVPFTAADPDYQEKYQELLEREDFFPDYEENGTDLSGAGDPYLDDIDDEMDPEIEEAYEKFCLESERKRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0J558</accession>
    <name>A0A9L0J558_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Enoyl-CoA hydratase domain containing 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">ECHDC2</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="4" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0J558"/>
    <dbReference type="Ensembl" id="ENSEAST00005074932.1">
        <property type="protein sequence ID" value="ENSEASP00005045147.1"/>
        <property type="gene ID" value="ENSEASG00005014472.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158798"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 5"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000022">
        <property type="entry name" value="methylglutaconyl-CoA hydratase, mitochondrial isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF44">
        <property type="entry name" value="ENOYL-COA HYDRATASE DOMAIN-CONTAINING PROTEIN 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="417" mass="45447" checksum="6177060B406C4F76" modified="2023-09-13" version="1">MLRPLTSALRLLRPWKPLQTRGCASDEAARGREIQVRALEGPDQGITEILMNRPRARNALGNVFVSELLEALAQLREDRQVRVLLFRSAVKGVFCAGADLKEREQMSEAEVEVFVQRLRGMMTEIAAFPAPTIAALDGFALGGGLELALACDLRVAAASTAVMGLIETTRGLLPGAGGTQRLPRCLGVALAKELIFTGRRLSGTQAQVLGLVNHAVAQNEEGNAAYHRARALAQEILPQAPIAVRLSKVAIDRGMEVDIASGMAIEGICYAQKNKFSARREYSGFCQEEQLSYQILEKSAPLKATGSPGLAVRRQVGYDTCCKIADFHFFALEQAADMLHHTLCHQLRGESSDFSHVVLQVRWSREYQGEERRGASGIGSSPSALTRSDTFRTLGPSLLPLYQLTPTIPPLDNEDDQ</sequence>
</entry>
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        <name type="common">Donkey</name>
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        <lineage>
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            <taxon>Mammalia</taxon>
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            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
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                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
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                <person name="Yin"/>
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                <person name="Jin"/>
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                <person name="Yue"/>
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                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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    <dbReference type="InterPro" id="IPR050169">
        <property type="entry name" value="Krueppel_C2H2_ZnF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232">
        <property type="entry name" value="KRAB DOMAIN C2H2 ZINC FINGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23232:SF102">
        <property type="entry name" value="KRAB DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF01352">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="6"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005044445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="126" mass="14699" checksum="B1726FD6E8293ED0" modified="2023-09-13" version="1">MAHSSMTFSDVAIDFSQQEWEGLDPVQKTLYQDVMVESYRNLVSLARHSNSKPDVITLLEQGKEPWMVVREETRRCYTRDARLGERGISQRICYLFYSTQTSSFLNLNEDESLSSPTLKSRRAYYL</sequence>
</entry>
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                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="5" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="5" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the learning-associated protein family.</text>
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    <dbReference type="AlphaFoldDB" id="A0A9L0J5K4"/>
    <dbReference type="Ensembl" id="ENSEAST00005081350.1">
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        <property type="gene ID" value="ENSEASG00005030974.1"/>
    </dbReference>
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    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001099">
        <property type="term" value="F:basal RNA polymerase II transcription machinery binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="term" value="P:positive regulation of dendritic spine development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018784">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR34253">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34253:SF1">
        <property type="entry name" value="PROTEIN LLP HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10169">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005048444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="124" mass="14618" checksum="4EE243D69AE88FF3" modified="2023-09-13" version="1">MAKSLRSKWKRKMRAEKRKKNAPKELSRLKSILKVDSDVLMKDVQEIATVVVPTECQEKTQCVVNDEKDDMKMETEIKRNKKSLLDQHGQYPIWMNPRQRKRLKAKRQKQRGRSKAKAAKGLAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JDR5</accession>
    <name>A0A9L0JDR5_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Ubiquitin carboxyl-terminal hydrolase</fullName>
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    <gene>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
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            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="J."/>
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                <person name="R."/>
                <person name="Hou"/>
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                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0JDR5"/>
    <dbReference type="Ensembl" id="ENSEAST00005070119.1">
        <property type="protein sequence ID" value="ENSEASP00005047370.1"/>
        <property type="gene ID" value="ENSEASG00005037948.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000157997"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140936">
        <property type="term" value="F:histone H2B deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051898">
        <property type="term" value="P:negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043491">
        <property type="term" value="P:phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000067">
        <property type="entry name" value="Ubiquitinyl hydrolase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001607">
        <property type="entry name" value="Znf_UBP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF7">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02148">
        <property type="entry name" value="zf-UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00290">
        <property type="entry name" value="ZnF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50271">
        <property type="entry name" value="ZF_UBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3 12" id="KW-0645">Protease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="6 12" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UBP-type" evidence="15">
        <location>
            <begin position="2"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="14">
        <location>
            <begin position="248"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="169"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="287"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="301"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="311"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005047370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="654" mass="74749" checksum="C9093FD5933AE0EB" modified="2023-09-13" version="1">MDRCKHVGRLRLAQDHSILNPQKWCCRECTTTESVWACLKCSHVACGRYIEDHALKHFEETGHPLAMEVWDLYVFCYLCKDYVLNDNPEGDLKLLRSSLLAVRGQKQDLPVRRGRMLRSMAAGEDAIPLQHAPQGQPQMLTALWYRRQRLLAKTLRLWFEKSSRGQAKLEQRRQEEALERKKEEARQRRREVKRRLLEELASAPPRKSARLLLHAPRAAAPAPRLAARGPAGGRLQPRRAPAMAPGVTGLRNLGNTCYMNSILQVLSHLQKFRECFLNLDPSKTEHLFPKTNGKAPLSGRPASSSATELSARSDRAESGEREGLCLNGGASISRSLELIQNKEPSSKHISLCHELHTLFRVMWSGKWALVSPFAMLHSVWSLIPAFRGYDQQDAQEFLCELLHKVQQELESEGTTHRILIPFSQRKLTKQVLKVVNTIFHGQLLSQVTCISCNYKSNTIEPFWDLSLEFPERYHCIEKGFIPLNQTECLLTEMLAKFTETEALEGRIYACDQCNSKRRKSNPKPLVLSEARKQLMIYRLPQVLRLHLKRFRWSGRNHREKIGVHVVFDQVLTMEPYCCRDMLSSLDKETFAYDLSAVVMHHGKGFGSGHYTAYCYNTEGGACALLCGVGDTEKGWLSTFHCVPFISSIKRYYIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JGE3</accession>
    <name>A0A9L0JGE3_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">LOC106835402</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JGE3"/>
    <dbReference type="Ensembl" id="ENSEAST00005041012.1">
        <property type="protein sequence ID" value="ENSEASP00005049077.1"/>
        <property type="gene ID" value="ENSEASG00005037015.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01140000282514"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 17"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15945">
        <property type="entry name" value="7tmA_OR5C1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000003">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000276">
        <property type="entry name" value="GPCR_Rhodpsn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017452">
        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000725">
        <property type="entry name" value="Olfact_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48018">
        <property type="entry name" value="OLFACTORY RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13853">
        <property type="entry name" value="7tm_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00237">
        <property type="entry name" value="GPCRRHODOPSN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00245">
        <property type="entry name" value="OLFACTORYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50262">
        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="98"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="140"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="197"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="238"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="275"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="domain" description="G-protein coupled receptors family 1 profile" evidence="10">
        <location>
            <begin position="41"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005049077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="312" mass="35242" checksum="12A1BBBBE0DBB07C" modified="2023-09-13" version="1">MDGENCSSLNEFLLLGISDNPGMKVTLFTTFLIVYLTILVANLGMIILIRIDSQLHTPVYFFLSHLSFSDLCYSTAIGPRMLVGFIAKKKSISFYGCALQWLIFCTFVDSECLLLVVMAFDWYKAISNPLLYTVNMSSRVCSLLMVVVYMTGIMDALINTILTFRLCFCGSNEINHFFCDIPPLLLLSCSDTQVNELVIFTIFGLIELVTLSGLFVSYCYIILAVIKIQSAEGRFKTFSTCTSHISVVAIFQGALLFMYFRPSSSYSLDQDKMTSLFYSLVCPMLNPLIYSLRNKDVKDALKKLKNKKWSHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JGM4</accession>
    <name>A0A9L0JGM4_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">UDP-glucuronosyltransferase</fullName>
            <ecNumber evidence="12">2.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">UGT1A6</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor beta-D-glucuronoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:132367"/>
            <dbReference type="ChEBI" id="CHEBI:132368"/>
            <dbReference type="EC" id="2.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Membrane</location>
            <topology evidence="12">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the UDP-glycosyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.17" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0JGM4"/>
    <dbReference type="Ensembl" id="ENSEAST00005047560.1">
        <property type="protein sequence ID" value="ENSEASP00005051493.1"/>
        <property type="gene ID" value="ENSEASG00005019912.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163820"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000001">
        <property type="entry name" value="UDP-glucuronosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000066">
        <property type="entry name" value="UDP-glucuronosyltransferase 1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050271">
        <property type="entry name" value="UDP-glycosyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035595">
        <property type="entry name" value="UDP_glycos_trans_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043">
        <property type="entry name" value="EG:EG0003.4 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48043:SF161">
        <property type="entry name" value="UDP GLUCURONOSYLTRANSFERASE FAMILY 1 MEMBER A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00201">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00375">
        <property type="entry name" value="UDPGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="490"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005051493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="532" mass="60707" checksum="12329F0606D01CFC" modified="2023-09-13" version="1">MACLLRAVWRVSAGVFFLSLWGMSVGDKLLVVPQDGSHWLSMKDIIEPLSEKGHDIVVLVPEVNLLLKESKYYTRRIYPVPYDKEEMVSRYRSFGKNHFAERWLLNAAQTEYRNVMIVIDMYFTNCQSLLNHSETLSFLRESKFDALFTDPALPCGVILADYLGLPSVYLFRGFPCSLEYAFTRSPNPVSYTPRCYTQFSDRMTFLQRVSNFLVSYLEKLLFYCLYSKYEELASHILKRDVHLPALYQKGSIWLLRYDSVFEYPRPVMPNMVFIGGVNCNKDGVLPQEFEAYVNASGEHGIVVFSLGSMVSEIPEKKAMEIADALGKIPQTVLWRYTGTPPPNLSKNTILVKWLPQNDLLGHPKTRAFITHSGSHGVYEGICNGVPMVMMPLFGDQMDNAKRMETRGAGVSLNVLEMTSDDLANALKTVINDKSYKENIMRLSSLHKDRPVEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLGVAFIIYKSCAFGFRKFFGKKGRVKKSHKSKTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JHB3</accession>
    <name>A0A9L0JHB3_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Semaphorin 3E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">SEMA3E</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the semaphorin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JHB3"/>
    <dbReference type="Ensembl" id="ENSEAST00005074090.1">
        <property type="protein sequence ID" value="ENSEASP00005052574.1"/>
        <property type="gene ID" value="ENSEASG00005003478.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106832427"/>
    <dbReference type="KEGG" id="eai:106832427"/>
    <dbReference type="CTD" id="9723"/>
    <dbReference type="GeneTree" id="ENSGT00940000158437"/>
    <dbReference type="OrthoDB" id="9988752at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045499">
        <property type="term" value="F:chemorepellent activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030215">
        <property type="term" value="F:semaphorin receptor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007411">
        <property type="term" value="P:axon guidance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001755">
        <property type="term" value="P:neural crest cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0030335">
        <property type="term" value="P:positive regulation of cell migration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="CDD" id="cd05871">
        <property type="entry name" value="Ig_Sema3"/>
        <property type="match status" value="1"/>
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    <dbReference type="CDD" id="cd11253">
        <property type="entry name" value="Sema_3E"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="2.130.10.10:FF:000015">
        <property type="entry name" value="Semaphorin 3B"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Semaphorin 3F like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1680.10">
        <property type="entry name" value="ligand-binding face of the semaphorins, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
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        <property type="entry name" value="Ig-like_dom"/>
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    <dbReference type="InterPro" id="IPR013151">
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    <dbReference type="InterPro" id="IPR016201">
        <property type="entry name" value="PSI"/>
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    <dbReference type="InterPro" id="IPR001627">
        <property type="entry name" value="Semap_dom"/>
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    <dbReference type="InterPro" id="IPR036352">
        <property type="entry name" value="Semap_dom_sf"/>
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    <dbReference type="InterPro" id="IPR027231">
        <property type="entry name" value="Semaphorin"/>
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    <dbReference type="InterPro" id="IPR015513">
        <property type="entry name" value="Semaphorin_3E_Sema"/>
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    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR11036">
        <property type="entry name" value="SEMAPHORIN"/>
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    <dbReference type="PANTHER" id="PTHR11036:SF22">
        <property type="entry name" value="SEMAPHORIN-3E"/>
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    <dbReference type="Pfam" id="PF00047">
        <property type="entry name" value="ig"/>
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    <dbReference type="SMART" id="SM00423">
        <property type="entry name" value="PSI"/>
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    <dbReference type="SMART" id="SM00630">
        <property type="entry name" value="Sema"/>
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    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103575">
        <property type="entry name" value="Plexin repeat"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF101912">
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50835">
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    <dbReference type="PROSITE" id="PS51004">
        <property type="entry name" value="SEMA"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
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    <feature type="chain" id="PRO_5040338942" evidence="9">
        <location>
            <begin position="26"/>
            <end position="776"/>
        </location>
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    <feature type="domain" description="Sema" evidence="11">
        <location>
            <begin position="32"/>
            <end position="516"/>
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    <feature type="domain" description="Ig-like" evidence="10">
        <location>
            <begin position="595"/>
            <end position="670"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
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            <dbReference type="ARBA" id="ARBA00004613"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009492"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
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            <dbReference type="ARBA" id="ARBA00023157"/>
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    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
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    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
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    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00352"/>
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    <evidence type="ECO:0000256" key="9">
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            <dbReference type="SAM" id="SignalP"/>
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    <evidence type="ECO:0000259" key="10">
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            <dbReference type="PROSITE" id="PS50835"/>
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            <dbReference type="Ensembl" id="ENSEASP00005052574.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="776" mass="89519" checksum="8C8A203BA612B133" modified="2023-09-13" version="1">MASAGHIVTWLLWGYLLELWTGGHTADTTHPRLRLSHKELLDLNRTSIFHSPFGFLDLHTMLLDEYQERLFVGGKDLIYSLSLEQINNGYREIHWPSTALKVEECIMKGKDTSECANYVRVLHHYNRTHLLTCGTGAFDPLCAFIRVGYHLEDPLFHLESHRWERGRGRCPFDPSSSFTSTLIGSELFTGLYSDYWGRDAAIFRSLGRLAHIRTEHDDERLLREPKFVGSHMIPDNEDGDDNKVYFFFTEKALEAENNAHAIYSRVGRLCVNDVGGQRMLVNKWSTFLKARLVCSVPGMNGIDTYFDELEDVFLLYTRDHKNPVIFGLFNTTSNIFRGHAVCVYHMSSIRAAFNGPYAHKEGPEYHWTLYEGKVPYPRPGSCASKVNGGRYGTTKDYPDDAIRFVRSHPLMYQPIKPAHKKPILVKTDGKYNLKQIAVDRVEAEDGQYDVLFIGTDNGIVLKVITIYNQETESMEEVILEELQIFKDPVPIISMEISSKRQQLYIGSTSTVAQVRFHQCDMYGSACADCCLARDPYCAWDGISCSRYYPTGSTHAKRRFRRQDVRHGDAAQQCFGQQFVGDALDKTEERLAYGIENNSTLLECTPRSLQAKVMWSVQKGHDTRKEEVKTDDRVVKMDLGLLFLRVHKMDAGTYFCQTVEHSFVHTVRKVTLEVVEEERVEEMFHKDYEEDGPHKMPCPAQSSIPQGVKPWYKEFLQLIGYSNFQRVEEYCEKVWCTEKKRKKLKMSPSKWKYANTQEKKLRPKAEHYRLPRHTLDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JIQ3</accession>
    <name>A0A9L0JIQ3_EQUAS</name>
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        <recommendedName>
            <fullName evidence="14">Immunoglobulin superfamily member 8</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">CD81 partner 3</fullName>
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        <alternativeName>
            <fullName evidence="15">Glu-Trp-Ile EWI motif-containing protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Prostaglandin regulatory-like protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="primary">IGSF8</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="21" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="21" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014703818.1">
        <property type="nucleotide sequence ID" value="XM_014848332.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044614156.1">
        <property type="nucleotide sequence ID" value="XM_044758221.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044614157.1">
        <property type="nucleotide sequence ID" value="XM_044758222.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044614158.1">
        <property type="nucleotide sequence ID" value="XM_044758223.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070353752.1">
        <property type="nucleotide sequence ID" value="XM_070497651.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070353753.1">
        <property type="nucleotide sequence ID" value="XM_070497652.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070353754.1">
        <property type="nucleotide sequence ID" value="XM_070497653.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070353755.1">
        <property type="nucleotide sequence ID" value="XM_070497654.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JIQ3"/>
    <dbReference type="Ensembl" id="ENSEAST00005077166.1">
        <property type="protein sequence ID" value="ENSEASP00005049892.1"/>
        <property type="gene ID" value="ENSEASG00005003188.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106835753"/>
    <dbReference type="KEGG" id="eai:106835753"/>
    <dbReference type="CTD" id="93185"/>
    <dbReference type="GeneTree" id="ENSGT00940000161314"/>
    <dbReference type="OMA" id="FIPCNVS"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000145">
        <property type="term" value="P:regulation of cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000191">
        <property type="entry name" value="Immunoglobulin superfamily member 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:000863">
        <property type="entry name" value="immunoglobulin superfamily member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007110">
        <property type="entry name" value="Ig-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036179">
        <property type="entry name" value="Ig-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003599">
        <property type="entry name" value="Ig_sub"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013106">
        <property type="entry name" value="Ig_V-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051102">
        <property type="entry name" value="IgSF_V-set/TM_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207:SF22">
        <property type="entry name" value="IMMUNOGLOBULIN SUPERFAMILY MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12207">
        <property type="entry name" value="V-SET AND TRANSMEMBRANE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="13" id="KW-0393">Immunoglobulin domain</keyword>
    <keyword evidence="12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="8 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0564">Palmitate</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 19" id="KW-0732">Signal</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="19">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040421667" description="Immunoglobulin superfamily member 8" evidence="19">
        <location>
            <begin position="21"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="573"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="6"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="domain" description="Ig-like" evidence="20">
        <location>
            <begin position="296"/>
            <end position="417"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00067284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00078280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00082384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005049892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="606" mass="64186" checksum="5E72A1D50406109B" modified="2023-09-13" version="1">MGALGPPLLLLLMLGVRCCAREVLVPKGPLYRVAGTAISISCNVSGYEGPAQQDFEWFLYRPEAPEAALGIVSTRDSRFSYAVFGPRVAAGEVQVQRLQGDAVVLKIARLQAQDAGIYECYTPSTDARYLGSYSGKVELRVLPDVLQVSAYPPGPRGRQAPASPPRLMVHEGQELALGCLARTSTQKHTHLAVSFGRAVPEAPVGRATLQEVVGLRPDLAVEAGAPYAERLAAGELRLGKEGTEQYRMVVGGAQAEDAGTYHCTAAEWIQDPDGSWAQIAEKRAVLAHVDVQTLSSQLAVAVGPGERRIGPGEPLELLCNVSGALPPPGRHAAYSVGWEMAPAGAPGPGRLVAQLDTEGVGSLGPGYEGRHIAMEKVASRTYRLRLEAARPGDAGTYRCLAKAYVRGSGARLREAASARSRPLPVHVREEGVVLEAVAWLAGGTVYRGETASLLCNISVRGGPPGLRLAASWWVERPEEGELSSAPAQLVGGVGRDGVAELGVRPGGGPVSVELVGPRSHRLRLHGLGPEDEGVYHCAPSAWVQHTDYSWYQAGSARSGPVTVYPYTHALDTLLVPLLVGAGVAFLTGAIVLGTITCCFMKRLRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JJA9</accession>
    <name>A0A9L0JJA9_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">ATP-dependent RNA helicase SUPV3L1, mitochondrial</fullName>
            <ecNumber evidence="5">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Suppressor of var1 3-like protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SUPV3L1</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="16">Major helicase player in mitochondrial RNA metabolism. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules. ATPase and ATP-dependent multisubstrate helicase, able to unwind double-stranded (ds) DNA and RNA, and RNA/DNA heteroduplexes in the 5'-to-3' direction. Plays a role in the RNA surveillance system in mitochondria; regulates the stability of mature mRNAs, the removal of aberrantly formed mRNAs and the rapid degradation of non coding processing intermediates. Also implicated in recombination and chromatin maintenance pathways. May protect cells from apoptosis. Associates with mitochondrial DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homodimer; in free form. Component of the mitochondrial degradosome (mtEXO) complex which is a heteropentamer containing 2 copies of SUPV3L1 and 3 copies of PNPT1. As part of mitochondrial degradosome complex, interacts with GRSF1 in a RNA-dependent manner; the interaction enhances the activity of the complex. Interacts with LAMTOR5/HBXIP, WRN and BLM.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
            <location evidence="3">Mitochondrion nucleoid</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="5"/>
    <dbReference type="RefSeq" id="XP_044612195.1">
        <property type="nucleotide sequence ID" value="XM_044756260.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JJA9"/>
    <dbReference type="Ensembl" id="ENSEAST00005049215.1">
        <property type="protein sequence ID" value="ENSEASP00005049285.1"/>
        <property type="gene ID" value="ENSEASG00005015607.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106845013"/>
    <dbReference type="CTD" id="6832"/>
    <dbReference type="GeneTree" id="ENSGT00390000003100"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045025">
        <property type="term" value="C:mitochondrial degradosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034458">
        <property type="term" value="F:3'-5' RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035946">
        <property type="term" value="P:mitochondrial mRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035945">
        <property type="term" value="P:mitochondrial ncRNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000965">
        <property type="term" value="P:mitochondrial RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043066">
        <property type="term" value="P:negative regulation of apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030307">
        <property type="term" value="P:positive regulation of cell growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000962">
        <property type="term" value="P:positive regulation of mitochondrial RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd17913">
        <property type="entry name" value="DEXQc_Suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18805">
        <property type="entry name" value="SF2_C_suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1740.140:FF:000001">
        <property type="entry name" value="ATP-dependent RNA helicase SUPV3L1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.1080:FF:000001">
        <property type="entry name" value="ATP-dependent RNA helicase SUPV3L1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000269">
        <property type="entry name" value="ATP-dependent RNA helicase SUPV3L1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000446">
        <property type="entry name" value="ATP-dependent RNA helicase SUPV3L1, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1740.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055206">
        <property type="entry name" value="DEXQc_SUV3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022192">
        <property type="entry name" value="SUV3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041082">
        <property type="entry name" value="Suv3_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044774">
        <property type="entry name" value="Suv3_DEXQc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041453">
        <property type="entry name" value="Suv3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE SUPV3L1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22527">
        <property type="entry name" value="DEXQc_Suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12513">
        <property type="entry name" value="SUV3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18147">
        <property type="entry name" value="Suv3_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18114">
        <property type="entry name" value="Suv3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0347">Helicase</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1135">Mitochondrion nucleoid</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="379"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="723"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="783"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="783"/>
            <end position="793"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00031873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00059444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00064833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005049285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="812" mass="90944" checksum="97EC56107D637521" modified="2023-09-13" version="1">MSFPRCALLWARLPAGRRAGHRAAVCSALRAHIGPFPGALGRVPAVASSSSASGGSKAPNTSLFVPLTVKPQGPSADGDVGAELTRPLDKNEVKKILDKFYKRKEIQKLAADYGLDARLFHQAFISFRNYIMQSHSLDVDIHIILNDICFSAAHVDDLFPFFLRHAKQIFPVLECKDDLRKISDLRIPPNWYPEARAIQRKIIFHSGPTNSGKTYHAIQKYLSAKSGVYCGPLKLLAHEIFEKSNAAGVPCDLVTGEERVTVEPDGKQAAHVACTVEMCSVTTPYEVAVIDEIQMIKDPARGWAWTRALLGLCAEEVHLCGESAAIDLVTELMYTTGEEVEVPDYVLCSQGYIANSPVPRQWLHRPQVRTYKRLTPITVLDHALESLDNLRPGDCIVCFSKNDIYSVSRQIEIRGLESAVIYGSLPPGTKLAQAKKFNDPDDPCKILVATDAIGMGLNLSIRRIIFYSLIKPSINEKGEKEIEPITTSQALQIAGRAGRFSSKFKEGEVTTMNREDLNLLREILNRPVDPIRAAGLHPTAEQIEMFAYHLPDTTLSNLIDIFVDFSQVDGQYFVCNMDDFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQFSRNEPLTFSWLRRYIKWPLLPPKNIKDLMDLEAVHDVLDLYLWLSYRFIDMFPDASFVRDLQKELDGIIQDGVHNITKLIKISETHKLLNLESLSTESQSRLSGTLKSQARRTRSTKTSGSKAAEPPSPHAGEKSLASRLVQQGLLTADMLKQLEKEWLTQRTEHGKEITESGTYSKGTRRKKKEPDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JK43</accession>
    <name>A0A9L0JK43_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Regulator of G-protein signaling 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">RGS5</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="7">Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="RefSeq" id="XP_014696656.1">
        <property type="nucleotide sequence ID" value="XM_014841170.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JK43"/>
    <dbReference type="SMR" id="A0A9L0JK43"/>
    <dbReference type="Ensembl" id="ENSEAST00005047305.1">
        <property type="protein sequence ID" value="ENSEASP00005050347.1"/>
        <property type="gene ID" value="ENSEASG00005024804.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106831139"/>
    <dbReference type="KEGG" id="eai:106831139"/>
    <dbReference type="CTD" id="8490"/>
    <dbReference type="GeneTree" id="ENSGT00940000157380"/>
    <dbReference type="OMA" id="FIIPYPD"/>
    <dbReference type="OrthoDB" id="196547at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 25"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009968">
        <property type="term" value="P:negative regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08717">
        <property type="entry name" value="RGS_RGS5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.167.10:FF:000001">
        <property type="entry name" value="Putative regulator of g-protein signaling 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.196.10:FF:000001">
        <property type="entry name" value="Regulator of G-protein signaling 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.196.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.167.10">
        <property type="entry name" value="Regulator of G-protein Signalling 4, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016137">
        <property type="entry name" value="RGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034956">
        <property type="entry name" value="RGS_RGS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036305">
        <property type="entry name" value="RGS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024066">
        <property type="entry name" value="RGS_subdom1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044926">
        <property type="entry name" value="RGS_subdomain_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845">
        <property type="entry name" value="REGULATOR OF G PROTEIN SIGNALING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10845:SF42">
        <property type="entry name" value="REGULATOR OF G-PROTEIN SIGNALING 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00615">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01301">
        <property type="entry name" value="RGSPROTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00315">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48097">
        <property type="entry name" value="Regulator of G-protein signaling, RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50132">
        <property type="entry name" value="RGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0734">Signal transduction inhibitor</keyword>
    <feature type="domain" description="RGS" evidence="8">
        <location>
            <begin position="64"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005050347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="181" mass="20952" checksum="4AC8A8E3AD273946" modified="2023-09-13" version="1">MCKGLAALPHSCLERAKEIKIKLGILLQKPDSAIDLVIPYNEKPEKPAKTQKPSLDEALQWRDSLDKVLQNNYGLASFKSFLKSEFSEENLEFWIACEDYKKIKSPDKMAEKAKKIYEEFIQTEAPKEVNIDHFTKDITMKNLVEPSPSSFDVAQKRIHALMEKDSLPRFVRSEFYQELIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JKZ6</accession>
    <name>A0A9L0JKZ6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Thioredoxin reductase 1, cytoplasmic</fullName>
            <ecNumber evidence="11">1.11.1.2</ecNumber>
            <ecNumber evidence="3">1.8.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Peroxidase TXNRD1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Thioredoxin reductase TR1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">TXNRD1</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="15">Reduces disulfideprotein thioredoxin (Trx) to its dithiol-containing form. Homodimeric flavoprotein involved in the regulation of cellular redox reactions, growth and differentiation. Contains a selenocysteine residue at the C-terminal active site that is essential for catalysis. Also has reductase activity on hydrogen peroxide (H2O2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>H2O2 + NADPH + H(+) = NADP(+) + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:15173"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16240"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.11.1.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="17">
            <dbReference type="Rhea" id="RHEA:15174"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
        <physiologicalReaction direction="right-to-left" evidence="16">
            <dbReference type="Rhea" id="RHEA:20347"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 18">Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.11.1.2" evidence="11"/>
    <dbReference type="EC" id="1.8.1.9" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0JKZ6"/>
    <dbReference type="Ensembl" id="ENSEAST00005075274.1">
        <property type="protein sequence ID" value="ENSEASP00005053904.1"/>
        <property type="gene ID" value="ENSEASG00005020848.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000160180"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004362">
        <property type="term" value="F:glutathione-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050137">
        <property type="term" value="F:NADPH peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008283">
        <property type="term" value="P:cell population proliferation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006749">
        <property type="term" value="P:glutathione metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001707">
        <property type="term" value="P:mesoderm formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000190">
        <property type="entry name" value="Thioredoxin reductase"/>
        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="3.30.390.30:FF:000004">
        <property type="entry name" value="Thioredoxin reductase 1, cytoplasmic"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046952">
        <property type="entry name" value="GSHR/TRXR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012999">
        <property type="entry name" value="Pyr_OxRdtase_I_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006338">
        <property type="entry name" value="Thioredoxin/glutathione_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01438">
        <property type="entry name" value="TGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737">
        <property type="entry name" value="GLUTATHIONE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42737:SF8">
        <property type="entry name" value="THIOREDOXIN-DISULFIDE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00076">
        <property type="entry name" value="PYRIDINE_REDOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5 18" id="KW-0274">FAD</keyword>
    <keyword evidence="4 18" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="8 18" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10 18" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0712">Selenocysteine</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="21">
        <location>
            <begin position="127"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="20">
        <location>
            <begin position="484"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="52"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="65"/>
            <end position="81"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    <evidence type="ECO:0000256" key="19">
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    <sequence length="601" mass="66010" checksum="F84B66994A58B634" modified="2023-09-13" version="1">MPVFEYWLCLPASHVRPCVQTVSVTQSRPHCCWFRGVLPVVPEPSCVPAMLPRGGHSAESPRPPSSTALPSTSQASYSTDPQLCPLPSTSHDRQEKNGQSGLASEEGRLQKLLKMNGSEDLPESYDYDLIIIGGGSGGLAAAKEAAKYNKKVMVLDFVTPTPLGTRWGLGGTCVNVGCIPKKLMHQAALLGQALRDSRNYGWNVEEMVKHDWEKMTEAVQNHIGSLNWGYRVALREKKVTYENAYGQFIGPHRIKTTNNKGKEKIYSAEKFLIATGERPRYLDIPGDKEYCISSDDLFSLPYCPGKTLVVGASYVALECAGFLAGIGLDVTVMVRSILLRGFDQDMANKIGEHMEEHGVKFIKQFVPIKVEQIEAGTPGRLRVVAQSTKGDETIEGEYNTVLLAIGRDACTRKIGLETVGVKINEKTGKIPVTDEEQTNVPYIYAIGDILEGKLELTPVAIQAGRLLAQRLYGGSTVKCDYENVPTTVFTPLEYGACGLSEENAVEKFGEENIEVYHSYFWPLEWTIPSRDNNKCYAKIICNIKDNERVVGFHVLGPNAGEVTQGFAAALKCGLTKQQLDSTIGIHPVCAEVGHLHFYCSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="13" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0JL76</accession>
    <name>A0A9L0JL76_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Homeobox expressed in ES cells 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">HESX1</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ANF homeobox family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014724139.1">
        <property type="nucleotide sequence ID" value="XM_014868653.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JL76"/>
    <dbReference type="Ensembl" id="ENSEAST00005057924.1">
        <property type="protein sequence ID" value="ENSEASP00005053128.1"/>
        <property type="gene ID" value="ENSEASG00005000319.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106848647"/>
    <dbReference type="KEGG" id="eai:106848647"/>
    <dbReference type="CTD" id="8820"/>
    <dbReference type="GeneTree" id="ENSGT00940000156780"/>
    <dbReference type="OMA" id="VNLCVHI"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
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        <property type="component" value="Chromosome 21"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043010">
        <property type="term" value="P:camera-type eye development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071276">
        <property type="term" value="P:cellular response to cadmium ion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070371">
        <property type="term" value="P:ERK1 and ERK2 cascade"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048853">
        <property type="term" value="P:forebrain morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010467">
        <property type="term" value="P:gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008406">
        <property type="term" value="P:gonad development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048861">
        <property type="term" value="P:leukemia inhibitory factor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035264">
        <property type="term" value="P:multicellular organism growth"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043584">
        <property type="term" value="P:nose development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030916">
        <property type="term" value="P:otic vesicle formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0021983">
        <property type="term" value="P:pituitary gland development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045995">
        <property type="term" value="P:regulation of embryonic development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048863">
        <property type="term" value="P:stem cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019827">
        <property type="term" value="P:stem cell population maintenance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030878">
        <property type="term" value="P:thyroid gland development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000214">
        <property type="entry name" value="Homeobox expressed in ES cells 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043402">
        <property type="entry name" value="Hesx1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46966">
        <property type="entry name" value="HOMEOBOX EXPRESSED IN ES CELLS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46966:SF1">
        <property type="entry name" value="HOMEOBOX EXPRESSED IN ES CELLS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="106"/>
            <end position="166"/>
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    <feature type="DNA-binding region" description="Homeobox" evidence="8">
        <location>
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        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006791"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00019285"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
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        <source>
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    </evidence>
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</entry>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
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                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
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                <person name="Yin"/>
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                <person name="Jin"/>
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                <person name="M."/>
                <person name="Wu"/>
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                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="2" key="3">
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            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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    <dbReference type="PANTHER" id="PTHR48195:SF1">
        <property type="entry name" value="RIKEN CDNA 2410002F23 GENE"/>
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            <end position="441"/>
        </location>
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            <end position="429"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005055806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="441" mass="47399" checksum="C58C6DD6A968C7D1" modified="2023-09-13" version="1">MTRSLQISEFCGHSNITVLLKSQCSSQLGLCPRRCVFKRRSHPPPPRLDVSSGTGAAPPSILEPGLGWSTTPAPFRLQVLSPGLEQPHRPSVLWPRPQASVPLPPGAPVAMAEGALAPTEVGRGDRRYTHTELLAVSQRFQQNPSELLITWVLRVYDQGGSALTLSPGELTLLGDLTSDTVFNYRCKALRGGRQPLLTWLLLAWRQRWESFLHFNSTELPFQPWTTMEEGIQLVRELGMLDWIYSELPLPATPEDVPFTPGLQQRLLTAAPSEARVSLVDLLVQGMTVLEAVMKIQAIRRRRPALASQPAGRRQARAGAQPDAQGPPGLAPEPRRAQGEGGQAAHQGPDGTLHQRGQAQPWPPGQSAGGQAAAAATTGASQLGPQGLAFHGPRSGGGRPRSGGANRAGGEHLKGHESPARPQDRLRDHLPACSPAYCGQAP</sequence>
</entry>
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    <accession>A0A9L0JT69</accession>
    <name>A0A9L0JT69_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ig-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
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            <taxon>Equus</taxon>
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    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Jin"/>
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                <person name="Yue"/>
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                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
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                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subunit">
        <text evidence="7">Alpha-beta TR is a heterodimer composed of an alpha and beta chain; disulfide-linked. The alpha-beta TR is associated with the transmembrane signaling CD3 coreceptor proteins to form the TR-CD3 (TcR or TCR). The assembly of alpha-beta TR heterodimers with CD3 occurs in the endoplasmic reticulum where a single alpha-beta TR heterodimer associates with one CD3D-CD3E heterodimer, one CD3G-CD3E heterodimer and one CD247 homodimer forming a stable octameric structure. CD3D-CD3E and CD3G-CD3E heterodimers preferentially associate with TR alpha and TR beta chains, respectively. The association of the CD247 homodimer is the last step of TcR assembly in the endoplasmic reticulum and is required for transport to the cell surface.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="Ensembl" id="ENSEAST00005052726.1">
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Ig-like_dom_sf"/>
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    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Ig_V-set"/>
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    <dbReference type="InterPro" id="IPR051896">
        <property type="entry name" value="TCR_alpha_variable"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19339:SF5">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19339">
        <property type="entry name" value="T CELL RECEPTOR ALPHA VARIABLE 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07686">
        <property type="entry name" value="V-set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00409">
        <property type="entry name" value="IG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00406">
        <property type="entry name" value="IGv"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48726">
        <property type="entry name" value="Immunoglobulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50835">
        <property type="entry name" value="IG_LIKE"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1064">Adaptive immunity</keyword>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8" id="KW-0391">Immunity</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-1279">T cell receptor</keyword>
    <feature type="domain" description="Ig-like" evidence="9">
        <location>
            <begin position="19"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="10">
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
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    </evidence>
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</entry>
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    <name>A0A9L0JU78_EQUAS</name>
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    <gene>
        <name evidence="10" type="primary">CALHM1</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CALHM family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0JU78"/>
    <dbReference type="Ensembl" id="ENSEAST00005074979.1">
        <property type="protein sequence ID" value="ENSEASP00005053562.1"/>
        <property type="gene ID" value="ENSEASG00005028407.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01030000234610"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
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    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="term" value="P:ATP export"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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    <dbReference type="InterPro" id="IPR029569">
        <property type="entry name" value="CALHM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32261">
        <property type="entry name" value="CALCIUM HOMEOSTASIS MODULATOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32261:SF2">
        <property type="entry name" value="CALCIUM HOMEOSTASIS MODULATOR PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14798">
        <property type="entry name" value="Ca_hom_mod"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="182"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Ensembl" id="ENSEASP00005053562.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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            <fullName evidence="10">HEXIM P-TEFb complex subunit 1</fullName>
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    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
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                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
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                <person name="Liu"/>
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                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="10" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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    <comment type="similarity">
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    <dbReference type="Ensembl" id="ENSEAST00005063849.1">
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097322">
        <property type="term" value="F:7SK snRNA binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0004861">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106140">
        <property type="term" value="F:P-TEFb complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140416">
        <property type="term" value="F:transcription regulator inhibitor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002218">
        <property type="term" value="P:activation of innate immune response"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007507">
        <property type="term" value="P:heart development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0034244">
        <property type="term" value="P:negative regulation of transcription elongation by RNA polymerase II"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032897">
        <property type="term" value="P:negative regulation of viral transcription"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901798">
        <property type="term" value="P:positive regulation of signal transduction by p53 class mediator"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024872">
        <property type="entry name" value="HEXIM"/>
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    <dbReference type="PANTHER" id="PTHR13469">
        <property type="entry name" value="HEXAMETHYLENE BISACETAMIDE INDUCIBLE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13469:SF7">
        <property type="entry name" value="PROTEIN HEXIM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15313">
        <property type="entry name" value="HEXIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02094">
        <property type="entry name" value="HEXIMFAMILY"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="214"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="285"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="24"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="108"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="132"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="149"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="237"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005054157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="360" mass="40432" checksum="66D5E8139EE23AAD" modified="2023-09-13" version="1">MAEPLLSEYQHQPQTSNCTGAAAVHEERNPDRPPGAEERVPKEDSRWQSRASPKSDGRPGLEGEGSLEPQPPPLQTQMCPESGCLVEAGEKSHNGDDLSAGGAPPPVAGGEPRPGAEPLAQPCPDSEANKLGAPAAGGEEAWGQQQRQLGKKKHRRRPSKKKRHWKPYYKLTWEEKKKFDEKQSLRASRIRAEMFAKGQPVAPYNTTQFLMDDHDQEEPDLKTGLYPKRAAAKSDDTSDEDFMEEAGEEDGGSDGMGGDGSEFLQRDFSETYERYHAESLQNMSKQELIKEYLELEKCLSRMEDENNRLRLESKRLGGDDARVRELELELDRLRAENLQLLTENELHRQQERAPLSKFGD</sequence>
</entry>
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            <fullName evidence="3">Transmembrane protein with metallophosphoesterase domain</fullName>
        </submittedName>
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    <gene>
        <name evidence="3" type="primary">TMPPE</name>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_014851839.3"/>
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    <dbReference type="RefSeq" id="XP_014707326.1">
        <property type="nucleotide sequence ID" value="XM_014851840.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0JXZ2"/>
    <dbReference type="Ensembl" id="ENSEAST00005061252.1">
        <property type="protein sequence ID" value="ENSEASP00005053980.1"/>
        <property type="gene ID" value="ENSEASG00005031717.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106838004"/>
    <dbReference type="KEGG" id="eai:106838004"/>
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    <dbReference type="GeneTree" id="ENSGT00390000010260"/>
    <dbReference type="OrthoDB" id="783096at2759"/>
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        <property type="component" value="Chromosome 21"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="MPP_YkuE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Calcineurin-like_PHP"/>
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        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051158">
        <property type="entry name" value="Metallophosphoesterase_sf"/>
    </dbReference>
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        <property type="entry name" value="TRANSMEMBRANE PROTEIN WITH METALLOPHOSPHOESTERASE DOMAIN"/>
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    <dbReference type="PANTHER" id="PTHR31302">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN WITH METALLOPHOSPHOESTERASE DOMAIN-RELATED"/>
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        <property type="entry name" value="Metallophos"/>
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        <property type="entry name" value="Metallo-dependent phosphatases"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="84"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="112"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="207"/>
            <end position="388"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005053980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="447" mass="48707" checksum="AD3E32659D72DBB5" modified="2023-09-13" version="1">MAIFRQLSLGMKAALAAGTVFVSMIVSRSYLAGSLELRAWRWLLRLQLALFANSLMLIGSHYIWCSAVSNLSHSSAAESACFQLWKMAVVTFLALAHSSFFTMLFLVAEEPYLFSLAAYSCLGAYIIMVFFLCTLSGMEQACQLLAWRSGRVVSSLDKTRKLALRPALAVVVTVVLSVVGLLNAAQPPAVKTVEVPIHQLPPSMDNLKIVLLSDIHLGPTVGRTKMEMFVRMVNMLEPDVTVIVGDLCDSEASTLRTAVAPLGQLHSRLGTYFVTGNHEYYTSDVSNWFALLESLNVKPLHNENVKISAIRAQHGGGEDGDWICLAGVDDIEADVLHYSGHGMDLDKALGGCSPDHTTILLAHQPLAAKRALQARPDINLILSGHTHAGQIFPLNVAAYLLNPFFAGLYQVAQTTFVYVSPGTAYYGIPMRLGSRAEITELILQRAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K023</accession>
    <name>A0A9L0K023_EQUAS</name>
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        <recommendedName>
            <fullName evidence="12">Cysteine protease</fullName>
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        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">ATG4C</name>
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    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
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                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[protein]-C-terminal L-amino acid-glycyl-phosphatidylethanolamide + H2O = [protein]-C-terminal L-amino acid-glycine + a 1,2-diacyl-sn-glycero-3-phosphoethanolamine</text>
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            <dbReference type="Rhea" id="RHEA-COMP:17323"/>
            <dbReference type="Rhea" id="RHEA-COMP:17324"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:172940"/>
            <dbReference type="ChEBI" id="CHEBI:172941"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:67549"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the peptidase C54 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.22.-" evidence="12"/>
    <dbReference type="AlphaFoldDB" id="A0A9L0K023"/>
    <dbReference type="Ensembl" id="ENSEAST00005063702.1">
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        <property type="gene ID" value="ENSEASG00005008203.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00530000063000"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 16"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004197">
        <property type="term" value="F:cysteine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019786">
        <property type="term" value="F:protein-phosphatidylethanolamide deconjugating activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035973">
        <property type="term" value="P:aggrephagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000423">
        <property type="term" value="P:mitophagy"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046793">
        <property type="entry name" value="ATG4_LIR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005078">
        <property type="entry name" value="Peptidase_C54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046792">
        <property type="entry name" value="Peptidase_C54_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22624:SF38">
        <property type="entry name" value="CYSTEINE PROTEASE ATG4C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20166">
        <property type="entry name" value="ATG4_LIR"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Peptidase_C54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 12" id="KW-0645">Protease</keyword>
    <keyword evidence="9 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Peptidase C54 catalytic" evidence="13">
        <location>
            <begin position="77"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005058871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="418" mass="47889" checksum="509F1200263ECA96" modified="2023-09-13" version="1">MEATGTDEVDKLKTKFISAWNNMKYSWVLKTKTYFSRNSPVLLLGKCYHFKYEDENKLLPARSGCTIEDHIIAGNVEEFRKDFTSRIWLTYREEFPQIEGSTLTTDCGWGCTLRTGQMLLAQGLILHFLGRAWTWPDALNIENSDFESWTSNTVKKFTASFEASLSEERELKTPTISLKETIGRYSDDHEMRNEIYHRKIISWFGDSPLALFGLHQLIEYGKKSGKKAGDWYGPAVVAHILRKAVEEARHPDLQGITIYVAQDCTVYSSDVIDKQCASMASDHADDKAVIILVPVRLGGERTNTDYLDFVKGILSLEYCVGIIGGKPKQSYYFAGFQDDSLIYMDPHYCQSFVDVSIKDFPLEMLKISSKEKYPLFTFVNGHSRDYDFTSTAAKEDDLFSEDEKKRLKRFSTEEFVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K2F8</accession>
    <name>A0A9L0K2F8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein FAM110B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">FAM110B</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-05" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Centrosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAM110 family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_014713278.1">
        <property type="nucleotide sequence ID" value="XM_014857792.3"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044636110.1">
        <property type="nucleotide sequence ID" value="XM_044780175.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044636111.1">
        <property type="nucleotide sequence ID" value="XM_044780176.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044636112.1">
        <property type="nucleotide sequence ID" value="XM_044780177.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044636114.1">
        <property type="nucleotide sequence ID" value="XM_044780179.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_044636115.1">
        <property type="nucleotide sequence ID" value="XM_044780180.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070337326.1">
        <property type="nucleotide sequence ID" value="XM_070481225.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070337327.1">
        <property type="nucleotide sequence ID" value="XM_070481226.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070337328.1">
        <property type="nucleotide sequence ID" value="XM_070481227.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_070337329.1">
        <property type="nucleotide sequence ID" value="XM_070481228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A9L0K2F8"/>
    <dbReference type="Ensembl" id="ENSEAST00005042243.1">
        <property type="protein sequence ID" value="ENSEASP00005036541.1"/>
        <property type="gene ID" value="ENSEASG00005024095.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005043990.1">
        <property type="protein sequence ID" value="ENSEASP00005052115.1"/>
        <property type="gene ID" value="ENSEASG00005024095.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005049135.1">
        <property type="protein sequence ID" value="ENSEASP00005036188.1"/>
        <property type="gene ID" value="ENSEASG00005024095.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005050858.1">
        <property type="protein sequence ID" value="ENSEASP00005040937.1"/>
        <property type="gene ID" value="ENSEASG00005024095.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005061393.1">
        <property type="protein sequence ID" value="ENSEASP00005049252.1"/>
        <property type="gene ID" value="ENSEASG00005024095.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005062934.1">
        <property type="protein sequence ID" value="ENSEASP00005052127.1"/>
        <property type="gene ID" value="ENSEASG00005024095.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005067604.1">
        <property type="protein sequence ID" value="ENSEASP00005049541.1"/>
        <property type="gene ID" value="ENSEASG00005024095.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005079342.1">
        <property type="protein sequence ID" value="ENSEASP00005042156.1"/>
        <property type="gene ID" value="ENSEASG00005024095.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005081841.1">
        <property type="protein sequence ID" value="ENSEASP00005053268.1"/>
        <property type="gene ID" value="ENSEASG00005024095.1"/>
    </dbReference>
    <dbReference type="Ensembl" id="ENSEAST00005083448.1">
        <property type="protein sequence ID" value="ENSEASP00005055520.1"/>
        <property type="gene ID" value="ENSEASG00005024095.1"/>
    </dbReference>
    <dbReference type="GeneID" id="106841665"/>
    <dbReference type="KEGG" id="eai:106841665"/>
    <dbReference type="CTD" id="90362"/>
    <dbReference type="GeneTree" id="ENSGT00950000183056"/>
    <dbReference type="OMA" id="IASMKSP"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 12"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005813">
        <property type="term" value="C:centrosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025740">
        <property type="entry name" value="FAM110"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025741">
        <property type="entry name" value="FAM110_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025739">
        <property type="entry name" value="FAM110_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14758">
        <property type="entry name" value="AGAP005440-PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14758:SF2">
        <property type="entry name" value="PROTEIN FAM110B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14160">
        <property type="entry name" value="FAM110_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14161">
        <property type="entry name" value="FAM110_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Centrosome-associated FAM110 N-terminal" evidence="8">
        <location>
            <begin position="14"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Centrosome-associated FAM110 C-terminal" evidence="7">
        <location>
            <begin position="254"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="127"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="317"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="326"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00039346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005055520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="370" mass="40624" checksum="86B20E9C1BEE8BCB" modified="2023-09-13" version="1">MPTETLQTGSMVKPVSPAGTFTSAVPLRILNKGPDYFRRQAEPNPKRLSAVERLEADKAKYVKSQEVINAKQEPVKPAVLAKPPVCPATKRALGSPTLKVFGSSAKTESGVQRENLKLEILKNIINSSEGSSSGSGHKHSSRNWPPHRPDATDLHRHSFAESLKVYPTQGRGSPQESSSHVGRRLLDQSAESFLHVSHSSSDIRKVTSVKPLKAIPCSSSAPPLPPKPKVAAIATMKSPEADPVEPACGVSRRPSLQRSKSDLSDRYFRVDADVERFFNYCGLDPEELENLGMENFARANSDIISLNFRSASMISSDCEQSQDSNSDLRNDDSANDRVPYGISAIERNARIIKWLYSIKQARESQKVSHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="11" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0K3I8</accession>
    <name>A0A9L0K3I8_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">OR9A2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0K3I8"/>
    <dbReference type="Ensembl" id="ENSEAST00005066232.1">
        <property type="protein sequence ID" value="ENSEASP00005059363.1"/>
        <property type="gene ID" value="ENSEASG00005032307.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000163070"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 1"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000015">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <reference evidence="15" key="2">
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            <authorList>
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        <scope>IDENTIFICATION</scope>
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            <authorList>
                <consortium name="Ensembl"/>
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        <scope>IDENTIFICATION</scope>
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    <comment type="function">
        <text evidence="11">Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. Interacts with beta-1 and beta-2, but not with beta-3.</text>
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    <comment type="subunit">
        <text evidence="3">G proteins are composed of 3 units, alpha, beta and gamma.</text>
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    <comment type="subunit">
        <text evidence="12">G proteins are composed of 3 units; alpha, beta and gamma.</text>
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        <subcellularLocation>
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            <topology evidence="1 12">Lipid-anchor</topology>
            <orientation evidence="1 12">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the G protein gamma family.</text>
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        <property type="component" value="Chromosome 10"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000007">
        <property type="entry name" value="Guanine nucleotide-binding protein subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G-protein_gamma"/>
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        <property type="entry name" value="G-protein_gamma-like_dom"/>
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        <property type="entry name" value="GGL_sf"/>
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    <dbReference type="PANTHER" id="PTHR13809">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN GAMMA SUBUNIT"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
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        <property type="entry name" value="GPROTEING"/>
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        <property type="entry name" value="G_gamma"/>
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    </dbReference>
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        <property type="entry name" value="GGL"/>
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    </dbReference>
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        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50058">
        <property type="entry name" value="G_PROTEIN_GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 12" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="7 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
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    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="14">
        <location>
            <begin position="107"/>
            <end position="148"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="23"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="13">
        <location>
            <begin position="30"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="65"/>
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        </location>
    </feature>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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        <source>
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    <sequence length="148" mass="14740" checksum="D9BBC04C2451E224" modified="2023-09-13" version="1">ALVGGPRRGSGCQCRRGYHLGAEGSEAGFGQLGSPGGGGGPGRGAGAAASPPRAGAAPEGAERAQRRRRHGFRGQRARPAAPGGAAQAGGRRGADEGTENTVPLVGKVSQAAAELQQYCMQNACKDALLVGVPAGSNPFREPRSCALL</sequence>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
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                <person name="Y."/>
                <person name="Yang"/>
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                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
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                <person name="W."/>
                <person name="Zhang"/>
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                <person name="Yu"/>
                <person name="J."/>
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                <person name="Yin"/>
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                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
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                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
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                <person name="J."/>
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                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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            <authorList>
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        <source>
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        </source>
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    <accession>A0A9L0K7X8</accession>
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            <fullName evidence="9">Transcription initiation factor TFIID subunit 7</fullName>
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        <name type="scientific">Equus asinus</name>
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    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Guo"/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. TAF7 forms a promoter DNA binding subcomplex of TFIID, together with TAF1 and TAF2. Part of a TFIID complex containing TAF10 (TFIID alpha) and a TFIID complex lacking TAF10 (TFIID beta).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF7 family.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014850218.3"/>
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        <property type="gene ID" value="ENSEASG00005018132.2"/>
    </dbReference>
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    <dbReference type="KEGG" id="eai:106836952"/>
    <dbReference type="CTD" id="6879"/>
    <dbReference type="GeneTree" id="ENSGT00940000160861"/>
    <dbReference type="OMA" id="NESDEHH"/>
    <dbReference type="OrthoDB" id="153872at2759"/>
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        <property type="component" value="Chromosome 12"/>
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    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0001673">
        <property type="term" value="C:male germ cell nucleus"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071339">
        <property type="term" value="C:MLL1 complex"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033276">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035035">
        <property type="term" value="F:histone acetyltransferase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
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        <property type="term" value="F:histone H3K27me3 reader activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046966">
        <property type="term" value="F:nuclear thyroid hormone receptor binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042809">
        <property type="term" value="F:nuclear vitamin D receptor binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106140">
        <property type="term" value="F:P-TEFb complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001097">
        <property type="term" value="F:TFIIH-class transcription factor complex binding"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:estrogen receptor signaling pathway"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0042789">
        <property type="term" value="P:mRNA transcription by RNA polymerase II"/>
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        <property type="project" value="Ensembl"/>
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        <property type="term" value="P:negative regulation of MHC class I biosynthetic process"/>
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060261">
        <property type="term" value="P:positive regulation of transcription initiation by RNA polymerase II"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TAF7"/>
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        <property type="entry name" value="TAFII55_prot_cons_reg"/>
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    <dbReference type="PANTHER" id="PTHR12228">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID 55 KD SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12228:SF6">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04658">
        <property type="entry name" value="TAFII55_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01370">
        <property type="entry name" value="TAFII55_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0832">Ubl conjugation</keyword>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
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                <person name="Guo"/>
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    </evidence>
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    </evidence>
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    <sequence length="121" mass="12950" checksum="FCC6AF73B15CE456" modified="2023-09-13" version="1">MMAAAPIQQNGTHTGVPIDLDPPDSRKRPLEAPPEAGSTKRTNTGEDGQYFLKVLIPSYAAGSIIGKGGQTIVQLQKETGATIKLSKSKDFYPGADSCRRNCTWSCTEGISPLCHLQSETD</sequence>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
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        <text evidence="11">Homodimer; homodimerization is required for activity. Interacts (via C-terminus) with ZNF219; forming a complex that binds to the COL2A1 promoter and activates COL2A1 expression. Interacts with DDRGK1. Interacts with EP300/p300. Interacts with beta-catenin (CTNNB1); inhibiting CTNNB1 activity by competing with the binding sites of TCF/LEF within CTNNB1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
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    <dbReference type="Ensembl" id="ENSEAST00005052273.1">
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        <property type="project" value="Ensembl"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
    </dbReference>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0001228">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097157">
        <property type="term" value="F:pre-mRNA intronic binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034236">
        <property type="term" value="F:protein kinase A catalytic subunit binding"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097065">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003180">
        <property type="term" value="P:aortic valve morphogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060018">
        <property type="term" value="P:astrocyte fate commitment"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030282">
        <property type="term" value="P:bone mineralization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001658">
        <property type="term" value="P:branching involved in ureteric bud morphogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060532">
        <property type="term" value="P:bronchus cartilage development"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060070">
        <property type="term" value="P:canonical Wnt signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001502">
        <property type="term" value="P:cartilage condensation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061323">
        <property type="term" value="P:cell proliferation involved in heart morphogenesis"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098609">
        <property type="term" value="P:cell-cell adhesion"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071364">
        <property type="term" value="P:cellular response to epidermal growth factor stimulus"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071504">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071347">
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071260">
        <property type="term" value="P:cellular response to mechanical stimulus"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071300">
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
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    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
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    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
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    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00046554"/>
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    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
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    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005058425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="509" mass="56270" checksum="0C4CFE115392B19A" modified="2023-09-13" version="1">MNLLDPFMKMTDEQEKGLSGAPSPTMSEDSAGSPCPSGSGSDTENTRPQENTFPKGEPDLKKESEEDKFPVCIREAVSQVLKGYDWTLVPMPVRVNGSSKNKPHVKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLGKLWRLLNESEKRPFVEEAERLRVQHKKDHPDYKYQPRRRKSVKNGQAEAEEATEQTHISPNAIFKALQADSPHSSSGMSEVHSPGEHSGQSQGPPTPPTTPKTDVQPGKADLKREGRPLPEGGRQPPIDFRDVDIGELSSDVISNIETFDVNEFDQYLPPNGHPGVPATHGQVTYTGSYGISSTAATPAGAGHVWMSKQQAPPPPPQQPPQAPQAPQAPPQQQQAPQPQPQAPPQQQQAHTLTTLSSEPGQSQRTHIKTEQLSPSHYSEQQQHSPQQIAYSPFNLPHYSPSYPPITRSQYDYTDHQNSGSYYSHAAGQGSGLYSTFTYMNPAQRPMYTPIADTSGVPSIPQTHSPQHWEQPVYTQLTRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KCU3</accession>
    <name>A0A9L0KCU3_EQUAS</name>
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        <submittedName>
            <fullName evidence="2">Chromosome 20 open reading frame 204</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">C20orf204</name>
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    <organism evidence="2 3">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <dbReference type="AlphaFoldDB" id="A0A9L0KCU3"/>
    <dbReference type="Ensembl" id="ENSEAST00005045974.1">
        <property type="protein sequence ID" value="ENSEASP00005062629.1"/>
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    <dbReference type="GeneTree" id="ENSGT00640000092841"/>
    <dbReference type="OrthoDB" id="9451460at2759"/>
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        <property type="component" value="Chromosome 15"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040453770" evidence="1">
        <location>
            <begin position="25"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005062629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="223" mass="24040" checksum="5DC7E044E038E019" modified="2023-09-13" version="1">MVPPKPMVWVLLLALLGIVPRQAALRACSVPDVLHHYRAVIFEDLQAVVRRVGPGAEGTGPGSRDLYFIKKNLTGAATPQRRGLMGASCGAQKEHSILLSIASLGQTLRGAVAGGRRGALEKAAWAVAVRIEAVMQRHCWTLRQRSRRPERRPARRRGGRRRLLLRALDAVATCWEKLFALRAAGTDGSRPPGRRRRAGGPWIVGASPVGGRLRAPGLLGGLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2023-09-13" modified="2026-01-28" version="12" xmlns="https://uniprot.org/uniprot">
    <accession>A0A9L0KDW7</accession>
    <name>A0A9L0KDW7_EQUAS</name>
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        <recommendedName>
            <fullName evidence="14">Histone deacetylase complex subunit SAP30L</fullName>
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            <fullName evidence="16">Sin3 corepressor complex subunit SAP30L</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Sin3-associated protein p30-like</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="primary">SAP30L</name>
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    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
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                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
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                <person name="Y."/>
                <person name="Gao"/>
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                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
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                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
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                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
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                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Functions as a transcription repressor, probably via its interaction with histone deacetylase complexes. Involved in the functional recruitment of the class 1 Sin3-histone deacetylase complex (HDAC) to the nucleolus. Binds DNA, apparently without sequence-specificity, and bends bound double-stranded DNA. Binds phosphoinositol phosphates (phosphoinositol 3-phosphate, phosphoinositol 4-phosphate and phosphoinositol 5-phosphate) via the same basic sequence motif that mediates DNA binding and nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Interacts with components of the histone deacetylase complex SIN3A, HDAC1 and HDAC2. Binds histones and nucleosomes. Interacts with FEZ1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAP30 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A9L0KDW7"/>
    <dbReference type="Ensembl" id="ENSEAST00005050337.1">
        <property type="protein sequence ID" value="ENSEASP00005060692.1"/>
        <property type="gene ID" value="ENSEASG00005037967.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006633"/>
    <dbReference type="OMA" id="SDQICCL"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 9"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001650">
        <property type="term" value="C:fibrillar center"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000118">
        <property type="term" value="C:histone deacetylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044378">
        <property type="term" value="F:non-sequence-specific DNA binding, bending"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010314">
        <property type="term" value="F:phosphatidylinositol-5-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1800.30:FF:000001">
        <property type="entry name" value="Histone deacetylase complex subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1800.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024145">
        <property type="entry name" value="His_deAcase_SAP30/SAP30L"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038291">
        <property type="entry name" value="SAP30_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025718">
        <property type="entry name" value="SAP30_Sin3-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025717">
        <property type="entry name" value="SAP30_zn-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13286:SF5">
        <property type="entry name" value="HISTONE DEACETYLASE COMPLEX SUBUNIT SAP30L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13286">
        <property type="entry name" value="SAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13867">
        <property type="entry name" value="SAP30_Sin3_bdg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13866">
        <property type="entry name" value="zf-SAP30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Histone deacetylase complex subunit SAP30 zinc-finger" evidence="18">
        <location>
            <begin position="25"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Histone deacetylase complex subunit SAP30 Sin3 binding" evidence="19">
        <location>
            <begin position="113"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="84"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
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                <person name="Liu"/>
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                <person name="Jiang"/>
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                <person name="Y."/>
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                <person name="Zhuang"/>
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                <person name="J."/>
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                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
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                <person name="Zhang"/>
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                <person name="Yin"/>
                <person name="Q."/>
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                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
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                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
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                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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                <consortium name="Ensembl"/>
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        <property type="entry name" value="LEUKEMIA NUP98 FUSION PARTNER 1"/>
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            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="178" mass="20903" checksum="86F9817B43CA3D2C" modified="2023-09-13" version="1">MEHRDDDDEDVSFAKWMSSFWGHSWIEEDERGLRDRHRSQHASYRKTSLPCPFPVLPRITSSDSHPRRRSHEDQGFQCRTHTQDYRKCSGDGSFKEPLESTGRSHSKMQAFSESSEQQLCFRTKRSVSLGPESRQERNERECLRMETGSCKKAVERRSCKKTEHREAYMAALFEKGPK</sequence>
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        <name type="scientific">Equus asinus</name>
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    </organism>
    <reference evidence="26 27" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="J."/>
                <person name="Liu"/>
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                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
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                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="26" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="26" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>1D-myo-inositol 1,3,4-trisphosphate + H2O = 1D-myo-inositol 1,3-bisphosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:43392"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58414"/>
            <dbReference type="ChEBI" id="CHEBI:83242"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="20">
            <dbReference type="Rhea" id="RHEA:43393"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>1D-myo-inositol 3,4-bisphosphate + H2O = 1D-myo-inositol 3-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:43388"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:83241"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:43389"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Signal transduction; phosphatidylinositol signaling pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21">Interacts with INPP5F.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Early endosome membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="18">Postsynaptic density</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="5">Recycling endosome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the inositol 3,4-bisphosphate 4-phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.66" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4KQU4"/>
    <dbReference type="Ensembl" id="ENSEAST00005000272.2">
        <property type="protein sequence ID" value="ENSEASP00005000195.2"/>
        <property type="gene ID" value="ENSEASG00005000242.2"/>
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    <dbReference type="GeneTree" id="ENSGT00940000157360"/>
    <dbReference type="UniPathway" id="UPA00944"/>
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        <property type="component" value="Chromosome 6"/>
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    <dbReference type="GO" id="GO:0031901">
        <property type="term" value="C:early endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0014069">
        <property type="term" value="C:postsynaptic density"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055038">
        <property type="term" value="C:recycling endosome membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0016316">
        <property type="term" value="F:phosphatidylinositol-3,4-bisphosphate 4-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04048">
        <property type="entry name" value="C2A_Copine"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Type I inositol 3,4-bisphosphate 4-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="C2 domain"/>
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        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039034">
        <property type="entry name" value="INPP4"/>
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    <dbReference type="PANTHER" id="PTHR12187">
        <property type="entry name" value="AGAP000124-PA"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12187:SF4">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE-4-PHOSPHATASE TYPE I A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="12" id="KW-0967">Endosome</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="27" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0770">Synapse</keyword>
    <feature type="domain" description="C2" evidence="25">
        <location>
            <begin position="26"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00006306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00013037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
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            <dbReference type="ARBA" id="ARBA00022475"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
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            <dbReference type="ARBA" id="ARBA00023018"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023098"/>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00034105"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00080875"/>
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    </evidence>
    <evidence type="ECO:0000256" key="24">
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            <dbReference type="ARBA" id="ARBA00082036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
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    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000195.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="977" mass="110017" checksum="C6844DA6CCEAD75E" modified="2023-09-13" version="2">MTAREHSPRHGARARAMQRASTIDVAADMLGLSLAGNIQDPDEPILEFSLACSELHTPSLDRKPNSFVAVSVTTPPQAFWTKHAQTEIIEGTNNPIFLSSIAFFQDSLINQMTQIKLSVYDVKDRSQGTMYLLGSGTFVVKDLLQDSHHRLHLTLRSAESDRVGNITVIGWQMEEKSDQRPPVTRSLDTVNGRMVLPVDESLTEALGIRSKYASLRKDTLLKSVFGGAICRMYRFPTTDGNHLRILEQMAESVLSLHVPRQFVKLLLEEDAARVCELEELGDLSPCWESLRRQIVTQYQTIILIYQENLTDLHQYKGPSFKASSLKADKKLEFVPTNLHIQRMRVQDDGGSDQNYDIVTIGAPAAHCQGFKSGGLRKKLHKFEETKKHFEECCTSSSCQSIIYIPQDIVRAKEIIAQINTLKTQVSYYAERLSRAAKDRSATGLERTLAILADKTRQLVTVCDCKLLANSIHGLNAARPDYIASKASPTSTEEEQVMLRNDQDTLMARWTGRNSRSSLQVDWHEEEWEKVWLNVDKSLECIIQRVDKLLQKERLHGEGCEDVFPCAGSCTSKKGKRDSHAYWIRPEDPFCDAPSSPCPSTMPCAACHPHLTTHCSPPPEESSPGEWSEALYPLLTTLTDCVAMMSDKAKKAMVFLLMQDSAPTIATYLSLQYRRDVVFCQTLTALICGFIIKLRNCLHDDGFLRQLYTIGLLAQFESLLSTYGEELAMLEDMSLGIMDLRNVTFKVTQATSNASTDMLPVITGNRDGFNVRIPLPGPLFDALPREIQSGMLLRVQPVLFNVGINEQQTLAERFGDTSLQEVINVESLVRLNSYFEQFKEVLPEDCLPRSRSQTCLPELLRFLGQNVHARKNKNVDILWQAAEICRRLNGVRFTSCKSAKDRTAMSVTLEQCLILQHEHGMAPQVFTQALECMRSEGCRRENTMKNVGSRKYAFNSLQLKAFPKHYRPPEGTYGKVET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4KWR7</accession>
    <name>A0A8C4KWR7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP synthase peripheral stalk subunit d, mitochondrial</fullName>
            <shortName evidence="10">ATPase subunit d</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ATP5PD</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="10">Subunit d, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ATPase d subunit family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KWR7"/>
    <dbReference type="Ensembl" id="ENSEAST00005000333.2">
        <property type="protein sequence ID" value="ENSEASP00005000254.2"/>
        <property type="gene ID" value="ENSEASG00005000278.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000003582"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 13"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.280.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008689">
        <property type="entry name" value="ATP_synth_F0_dsu_mt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036228">
        <property type="entry name" value="ATP_synth_F0_dsu_sf_mt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12700">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT D, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05873">
        <property type="entry name" value="Mt_ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005514">
        <property type="entry name" value="ATPase_F0_D_mt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161065">
        <property type="entry name" value="ATP synthase D chain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5 10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR005514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005000254.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="165" mass="19010" checksum="9EF86C44BC79618C" modified="2023-09-13" version="2">MDCKMAGRKLALKAIDWVAFGEIIPRNQKAIANSLKSWNETLTSRLATLPEAPPAIDWAYYKANVAKAGLVDDFEKKFNALKVPVPEDKYTVQVDAEEKEDVKSCAEFLSLSKARIEEYEKELEKMKNIIPFDQMTIEDLNEVFPETKLDKKKYPYWPHKPIENL</sequence>
</entry>
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    <accession>A0A8C4KXN7</accession>
    <name>A0A8C4KXN7_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Olfactory receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">LOC106836029</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="16" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="16" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="1">Putative odorant or sperm cell receptor.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Cell membrane</location>
            <topology evidence="2 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the G-protein coupled receptor 1 family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4KXN7"/>
    <dbReference type="Ensembl" id="ENSEAST00005003331.2">
        <property type="protein sequence ID" value="ENSEASP00005003050.2"/>
        <property type="gene ID" value="ENSEASG00005030952.1"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT01010000222320"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 20"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004984">
        <property type="term" value="F:olfactory receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd15405">
        <property type="entry name" value="7tmA_OR8B-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1220.70:FF:000001">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000004">
        <property type="entry name" value="Olfactory receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </evidence>
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    <sequence length="316" mass="35400" checksum="A86D651A6D29ED5E" modified="2023-09-13" version="2">LSQSKRMASGNCSFVTEFILLGLTDQPDLQLPLFFLFLVMYMVTVLGNLGLITLTGLNSRLHTPMYFFLLNLSFIDFCYSSVFTPKMLMDFLSKKNIISYLGCMSQLFFFCFFAISEGYVLTSMAYDRYVAICNPLLYNIAMSPKVCSSLMLGSYLMAFSGATAHTGCMLRLTFCDANTINHYFCDILPVLQLSCTSTYVNELVVFIVVGINIIVPSLTIFVSYGFILSTILHISSMEGRSKAFSTCSSHIIAVSLFFGSCAFMYLKPSSAMSMDEGKISSVFYTNVVPMANPLIYSLRNKDVKIALRKILRKRTF</sequence>
</entry>
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    <accession>A0A8C4KZD8</accession>
    <name>A0A8C4KZD8_EQUAS</name>
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        <recommendedName>
            <fullName evidence="12">Olfactory receptor</fullName>
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    </protein>
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        <name evidence="14" type="primary">LOC106830372</name>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Metazoa</taxon>
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            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
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                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
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                <person name="Yin"/>
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                <person name="Ji"/>
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                <person name="Jin"/>
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                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
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                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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        <text evidence="1">Odorant receptor.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Putative odorant or sperm cell receptor.</text>
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        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
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        <subcellularLocation>
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    <comment type="similarity">
        <text evidence="11">Belongs to the G-protein coupled receptor 1 family.</text>
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        <property type="gene ID" value="ENSEASG00005024255.1"/>
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        <property type="component" value="Chromosome 20"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="7tmA_OR52A-like"/>
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        <property type="entry name" value="Olfactory receptor"/>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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    </dbReference>
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        <property type="entry name" value="GPCR_Rhodpsn"/>
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        <property type="entry name" value="GPCR_Rhodpsn_7TM"/>
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        <property type="entry name" value="Olfact_rcpt"/>
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    <dbReference type="InterPro" id="IPR050402">
        <property type="entry name" value="OR51/52/56-like"/>
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        <property type="entry name" value="OLFACTORY RECEPTOR 52A5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OLFACTORY RECEPTOR 56B1-RELATED"/>
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        <property type="entry name" value="G_PROTEIN_RECEP_F1_2"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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        <location>
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    </feature>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU363047"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="339" mass="38390" checksum="0820F9809C76B119" modified="2023-09-13" version="2">MKSCHGIEDETGLSLLGSADHTTMLIFNGSIFMPSVLTLIGIPGLESVQCWIGIPFSVMYLIAVIGNSLILVIIKYENSLHQPMYIFLAILGATDMALSTCILPKMLGIFWFHLPEISFDACLLQMWFIHLFQGIESGVLLAMALDRYVAICDPLRHATIFSQQLLTYMGVGVTLRAAILTAPCLVLIKCRLKLYRTTTISHSYCEHMAIVKLATEDIRINKIYGLFVAFTILGFDIIFITLSYVQIFITVFRLPQKEARFKAFNTCIAHICVFLQFYLLAFFSFFTHRFGSQIPPYVHILLSNLYLLVPTFLNPIVYGVKTKQIRAHVLKMFFSKKTP</sequence>
</entry>
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    <accession>A0A8C4L023</accession>
    <name>A0A8C4L023_EQUAS</name>
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        <recommendedName>
            <fullName evidence="6">Transcription factor SOX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">SOX12</name>
    </gene>
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        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Metazoa</taxon>
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            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
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                <person name="A."/>
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                <person name="M."/>
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                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <scope>IDENTIFICATION</scope>
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        <name type="scientific">Equus asinus</name>
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            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
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                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
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                <person name="Guo"/>
                <person name="J."/>
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                <person name="Fan"/>
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                <person name="Zhang"/>
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                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
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        </citation>
        <scope>IDENTIFICATION</scope>
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    <reference evidence="11" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
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    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
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        <text evidence="2">Belongs to the alpha-carbonic anhydrase family.</text>
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        <property type="project" value="TreeGrafter"/>
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        <property type="project" value="UniProtKB-EC"/>
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        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Alpha carbonic anhydrase"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR001148">
        <property type="entry name" value="CA_dom"/>
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        <property type="entry name" value="CA_dom_sf"/>
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    <dbReference type="InterPro" id="IPR023561">
        <property type="entry name" value="Carbonic_anhydrase_a-class"/>
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    <dbReference type="PANTHER" id="PTHR18952">
        <property type="entry name" value="CARBONIC ANHYDRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18952:SF84">
        <property type="entry name" value="CARBONIC ANHYDRASE 14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00194">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01057">
        <property type="entry name" value="Carb_anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51069">
        <property type="entry name" value="Carbonic anhydrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51144">
        <property type="entry name" value="ALPHA_CA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5040474461" description="carbonic anhydrase" evidence="9">
        <location>
            <begin position="19"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-carbonic anhydrase" evidence="10">
        <location>
            <begin position="20"/>
            <end position="278"/>
        </location>
    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005001946.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="335" mass="37224" checksum="5AC051BB3D94EA49" modified="2023-09-13" version="2">MLFFSLLLELLWILAADGGQHWTYEGPHGQDHWPASYPECGSNAQSPINIQTDSVTFDPELPALQPHGYDEPGTEPLDMHNNGHTVQLSLPPALYLEGLPQKYVAAQLHLHWGQKGAPGGSEHQINSKATAAELHIVHYDSDSYDSLSEAAQRPQGLAVLGILIEAGETKNPAYEHILGHLHEIRHKDEKTSVPPFNVGELLPPQLGQFFRYNGSLTTPPCYQSVLWTVFNQRAQISMEQLEKLQETLFSTEGEPPELLVQNYRAPQPLNQRTVFASFIQGFLYTTGEMLSLGVGIMVGCLCLLLAVYFIARKIRKKRMGSRKSVVFTSARATEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L127</accession>
    <name>A0A8C4L127_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="23">DNA polymerase eta</fullName>
            <ecNumber evidence="5">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="26">RAD30 homolog A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="30" type="primary">POLH</name>
    </gene>
    <organism evidence="30 31">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="30 31" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="30" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="30" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="25">Interacts with REV1. Interacts with monoubiquitinated PCNA, but not unmodified PCNA. Interacts with POLI; this interaction targets POLI to the replication machinery. Interacts with PALB2 and BRCA2; the interactions are direct and are required to sustain the recruitment of POLH at blocked replication forks and to stimulate POLH-dependent DNA synthesis on D loop substrates. Interacts (via C-terminus) with TRAIP. Interacts with ubiquitin. Interacts with POLDIP2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DNA polymerase type-Y family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="5"/>
    <dbReference type="RefSeq" id="XP_014692925.1">
        <property type="nucleotide sequence ID" value="XM_014837439.3"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4L127"/>
    <dbReference type="Ensembl" id="ENSEAST00005004876.2">
        <property type="protein sequence ID" value="ENSEASP00005004448.1"/>
        <property type="gene ID" value="ENSEASG00005003364.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106828740"/>
    <dbReference type="KEGG" id="eai:106828740"/>
    <dbReference type="CTD" id="5429"/>
    <dbReference type="GeneTree" id="ENSGT00940000157048"/>
    <dbReference type="OMA" id="QNHRVAK"/>
    <dbReference type="OrthoDB" id="5723at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071494">
        <property type="term" value="P:cellular response to UV-C"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000731">
        <property type="term" value="P:DNA synthesis involved in DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006290">
        <property type="term" value="P:pyrimidine dimer repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd01702">
        <property type="entry name" value="PolY_Pol_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.100:FF:000007">
        <property type="entry name" value="DNA polymerase eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.270:FF:000022">
        <property type="entry name" value="DNA polymerase eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1170.60:FF:000003">
        <property type="entry name" value="DNA polymerase eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000014">
        <property type="entry name" value="Polymerase (DNA directed), eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017961">
        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052230">
        <property type="entry name" value="DNA_polymerase_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041298">
        <property type="entry name" value="UBZ3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873:SF1">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11799">
        <property type="entry name" value="IMS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21704">
        <property type="entry name" value="POLH-Rev1_HhH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18439">
        <property type="entry name" value="zf_UBZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036603">
        <property type="entry name" value="DPol_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51907">
        <property type="entry name" value="ZF_UBZ3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0227">DNA damage</keyword>
    <keyword evidence="20" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0235">DNA replication</keyword>
    <keyword evidence="8" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="19" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="18" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="7" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0515">Mutator protein</keyword>
    <keyword evidence="10" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="21" id="KW-0539">Nucleus</keyword>
    <keyword evidence="31" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="22" id="KW-0704">Schiff base</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="17" id="KW-0832">Ubl conjugation</keyword>
    <keyword evidence="15" id="KW-0862">Zinc</keyword>
    <keyword evidence="14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="UmuC" evidence="28">
        <location>
            <begin position="9"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="domain" description="UBZ3-type" evidence="29">
        <location>
            <begin position="627"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="449"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="489"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="27">
        <location>
            <begin position="667"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="27">
        <location>
            <begin position="456"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="27">
        <location>
            <begin position="667"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00044975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00064665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00080427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="29">
        <source>
            <dbReference type="PROSITE" id="PS51907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005004448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="31">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4L517</accession>
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        <submittedName>
            <fullName evidence="13">Adhesion G protein-coupled receptor F4</fullName>
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            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
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            <taxon>Equus</taxon>
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        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
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                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
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                <person name="Min"/>
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                <person name="Wang"/>
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                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="13" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.</text>
    </comment>
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        <property type="nucleotide sequence ID" value="XM_014834685.3"/>
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        <property type="nucleotide sequence ID" value="XM_070514789.1"/>
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        <property type="protein sequence ID" value="ENSEASP00005005456.1"/>
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    <dbReference type="GeneTree" id="ENSGT00940000161797"/>
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        <property type="component" value="Chromosome 8"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
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        <property type="project" value="TreeGrafter"/>
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    </dbReference>
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        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
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        <property type="entry name" value="GPCR_2-like_7TM"/>
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        <property type="entry name" value="GPCR_2_Ig-hepta-like_rcpt"/>
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    <dbReference type="InterPro" id="IPR000832">
        <property type="entry name" value="GPCR_2_secretin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000203">
        <property type="entry name" value="GPS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45813:SF1">
        <property type="entry name" value="ADHESION G PROTEIN-COUPLED RECEPTOR F4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45813">
        <property type="entry name" value="IG-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00002">
        <property type="entry name" value="7tm_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01825">
        <property type="entry name" value="GPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00249">
        <property type="entry name" value="GPCRSECRETIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01695">
        <property type="entry name" value="IGHEPTARCPTR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="G_PROTEIN_RECEP_F2_4"/>
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    </dbReference>
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        <property type="entry name" value="GAIN_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0732">Signal</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5034251808" evidence="10">
        <location>
            <begin position="24"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="398"/>
            <end position="420"/>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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    <feature type="transmembrane region" description="Helical" evidence="9">
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="630"/>
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            <begin position="247"/>
            <end position="395"/>
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    </feature>
    <feature type="domain" description="G-protein coupled receptors family 2 profile 2" evidence="12">
        <location>
            <begin position="399"/>
            <end position="654"/>
        </location>
    </feature>
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            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        </source>
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</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
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    <name>A0A8C4L8L6_EQUAS</name>
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        <submittedName>
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            <taxon>Equus</taxon>
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    </organism>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    <dbReference type="FunFam" id="3.10.100.10:FF:000044">
        <property type="entry name" value="CD209 antigen, isoform CRA_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.100.10">
        <property type="entry name" value="Mannose-Binding Protein A, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001304">
        <property type="entry name" value="C-type_lectin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016186">
        <property type="entry name" value="C-type_lectin-like/link_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018378">
        <property type="entry name" value="C-type_lectin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051379">
        <property type="entry name" value="C-type_Lectin_Receptor_IMM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033989">
        <property type="entry name" value="CD209-like_CTLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016187">
        <property type="entry name" value="CTDL_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46746:SF9">
        <property type="entry name" value="CD209 ANTIGEN-LIKE PROTEIN C-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46746">
        <property type="entry name" value="KILLER CELL LECTIN-LIKE RECEPTOR SUBFAMILY F MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00059">
        <property type="entry name" value="Lectin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00034">
        <property type="entry name" value="CLECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56436">
        <property type="entry name" value="C-type lectin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00615">
        <property type="entry name" value="C_TYPE_LECTIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50041">
        <property type="entry name" value="C_TYPE_LECTIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0430">Lectin</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="C-type lectin" evidence="5">
        <location>
            <begin position="114"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005005124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="238" mass="26801" checksum="CFE9D09DCF94E66E" modified="2022-01-19" version="1">MDQRDPKEPMALDEEELMTSGNRLSSKDFGFQKSHGFKNFPGCQGQGRAPLVLLFLSLVVFAGLLVAILVQVSKVPRSQGLEPSKQEEIQQILTQLKAGIALLCHPCPWGWRSFQGNCYFFSNSQLNWHDSVTACEEVEAQLVVIESAEEQNFLQVQASRFNRLTWMGLSDLKHEGTWHWVDGSPLLPSFMKYWNEGEPNSSGEEDCAEFRGKGWNDSKCSLSKFWICKKPTTSCSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LB46</accession>
    <name>A0A8C4LB46_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Creatine kinase U-type, mitochondrial</fullName>
            <ecNumber evidence="3">2.7.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Acidic-type mitochondrial creatine kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Ubiquitous mitochondrial creatine kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="primary">LOC106835019</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="22" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="22" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="12">Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Exists as an octamer composed of four MTCK homodimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Intermembrane side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 17 19">Belongs to the ATP:guanido phosphotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.3.2" evidence="3"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4LB46"/>
    <dbReference type="Ensembl" id="ENSEAST00005009188.2">
        <property type="protein sequence ID" value="ENSEASP00005008431.1"/>
        <property type="gene ID" value="ENSEASG00005005900.2"/>
    </dbReference>
    <dbReference type="KEGG" id="eai:106835019"/>
    <dbReference type="GeneTree" id="ENSGT00950000182772"/>
    <dbReference type="OMA" id="NCMASAM"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004111">
        <property type="term" value="F:creatine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901029">
        <property type="term" value="P:negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046314">
        <property type="term" value="P:phosphocreatine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00716">
        <property type="entry name" value="creatine_kinase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.135.10:FF:000002">
        <property type="entry name" value="creatine kinase S-type, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000002">
        <property type="entry name" value="Creatine kinase S-type, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.135.10">
        <property type="entry name" value="ATP:guanido phosphotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000749">
        <property type="entry name" value="ATP-guanido_PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022415">
        <property type="entry name" value="ATP-guanido_PTrfase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022414">
        <property type="entry name" value="ATP-guanido_PTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022413">
        <property type="entry name" value="ATP-guanido_PTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036802">
        <property type="entry name" value="ATP-guanido_PTrfase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547">
        <property type="entry name" value="ARGININE OR CREATINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11547:SF24">
        <property type="entry name" value="CREATINE KINASE U-TYPE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00217">
        <property type="entry name" value="ATP-gua_Ptrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02807">
        <property type="entry name" value="ATP-gua_PtransN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48034">
        <property type="entry name" value="Guanido kinase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00112">
        <property type="entry name" value="PHOSPHAGEN_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51510">
        <property type="entry name" value="PHOSPHAGEN_KINASE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51509">
        <property type="entry name" value="PHOSPHAGEN_KINASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 18" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 18 19" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 18 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Phosphagen kinase N-terminal" evidence="20">
        <location>
            <begin position="45"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphagen kinase C-terminal" evidence="21">
        <location>
            <begin position="158"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="161"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="325"/>
            <end position="329"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="353"/>
            <end position="358"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00041417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00041802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU000505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005008431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="417" mass="47028" checksum="044D2D98CF520853" modified="2022-01-19" version="1">MAGPFSRLLSARPGLRLLALAGAGSLAAGILLRPEPVRAASERRRLYPPSAEYPDLRKHNNCMASHLTPAVYARLCDKTTPTGWTLDQCIQTGVDNPGHPFIKTVGMVAGDEETYEVFAELFDPVIQERHNGYDPRTMKHTTDLDASKIRSGYFDERYVLSSRVRTGRSIRGLSLPPACTRAERREVERVVVDALSGLKGDLAGRYYRLSEMTEAEQQQLIDDHFLFDKPVSPLLTAAGMARDWPDARGIWHNNEKSFLIWVNEEDHTRVISMEKGGNMKKVFERFCRGLKEVERLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHIKLPLLSKDSRFPKILENLRLQKRGTGGVDTAATGSVFDISNLDRLGKSEVELVQLVIDGVNYLIDCERRLERGQDIRIPPPLVHNKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LC76</accession>
    <name>A0A8C4LC76_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">cyclin-dependent kinase</fullName>
            <ecNumber evidence="4">2.7.11.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Mediator complex subunit cdk-8</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Mediator of RNA polymerase II transcription subunit cdk-8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">CDK19</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.22"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.22" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4LC76"/>
    <dbReference type="Ensembl" id="ENSEAST00005010447.2">
        <property type="protein sequence ID" value="ENSEASP00005009621.2"/>
        <property type="gene ID" value="ENSEASG00005006723.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158213"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000707">
        <property type="entry name" value="Cyclin dependent kinase 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000088">
        <property type="entry name" value="cyclin-dependent kinase 8 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF570">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="18">
        <location>
            <begin position="21"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="390"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="17">
        <location>
            <begin position="436"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="452"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="486"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="496"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00053807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00083351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005009621.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="530" mass="60121" checksum="E37EEF59B2FA2C48" modified="2023-09-13" version="2">MDYDFKAKLAAERERVEDLFEYEGCKVGRGTYGHVYKARRKDGKDEKEYALKQIEGTGISMSACREIAINRIRSWDSMIASYFSRWISGEKELSFGLLRELKHPNVIALQKVFLSHSDRKVWLLFDYAEHDLWHIIKFHRASKANKKPMQLPRSMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFARLFNSPLKPLADLDPVVVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEDIKTSNPFHHDQLDRIFSVMGFPADKDWEDIRKMPEYPTLQKDFRRTTYANSSLIKYMEKHKVKPDSKVFLLLQKLLTMDPTKRITSEQALQDPYFQEDPLPTLDVFAGCQIPYPKREFLNEDEPEEKGDKNQQQQQNQHQQPTAPPQQAAAPPQAPPPQQNSTQTNGTAGGAGAGAGGTGAGLQHSQDSSLNQVPPNKKPRLGPSGANSGGPVMPSDYQHSSSRLNYQSSVQGSSQSQSTLGYSSSSQQSTQYHPSHQAHRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LDV0</accession>
    <name>A0A8C4LDV0_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">CREB regulated transcription coactivator 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">CRTC3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the TORC family.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044616920.1">
        <property type="nucleotide sequence ID" value="XM_044760985.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LDV0"/>
    <dbReference type="Ensembl" id="ENSEAST00005010501.2">
        <property type="protein sequence ID" value="ENSEASP00005009671.2"/>
        <property type="gene ID" value="ENSEASG00005006853.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106832053"/>
    <dbReference type="KEGG" id="eai:106832053"/>
    <dbReference type="CTD" id="64784"/>
    <dbReference type="GeneTree" id="ENSGT00390000010652"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="term" value="F:cAMP response element binding protein binding"/>
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        <property type="term" value="P:lipid catabolic process"/>
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        <property type="project" value="Ensembl"/>
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    <dbReference type="GO" id="GO:0042116">
        <property type="term" value="P:macrophage activation"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0071878">
        <property type="term" value="P:negative regulation of adenylate cyclase-activating adrenergic receptor signaling pathway"/>
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        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050995">
        <property type="term" value="P:negative regulation of lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051289">
        <property type="term" value="P:protein homotetramerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024786">
        <property type="entry name" value="TORC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024785">
        <property type="entry name" value="TORC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024784">
        <property type="entry name" value="TORC_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024783">
        <property type="entry name" value="TORC_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13589">
        <property type="entry name" value="CREB-REGULATED TRANSCRIPTION COACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13589:SF4">
        <property type="entry name" value="CREB-REGULATED TRANSCRIPTION COACTIVATOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12886">
        <property type="entry name" value="TORC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12885">
        <property type="entry name" value="TORC_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12884">
        <property type="entry name" value="TORC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0010">Activator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transducer of regulated CREB activity N-terminal" evidence="11">
        <location>
            <begin position="11"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Transducer of regulated CREB activity middle" evidence="12">
        <location>
            <begin position="159"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Transducer of regulated CREB activity C-terminal" evidence="13">
        <location>
            <begin position="545"/>
            <end position="619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="368"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="340"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="368"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="405"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="443"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="455"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007167"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
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    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
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            <dbReference type="ARBA" id="ARBA00023159"/>
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            <dbReference type="ARBA" id="ARBA00023163"/>
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    <evidence type="ECO:0000256" key="10">
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    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF12885"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
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    </evidence>
    <sequence length="619" mass="67062" checksum="051FBF0164E34FEF" modified="2023-09-13" version="2">MAASPGSGSANPRKFSEKIALHTQRQAEETRAFEQLMTDLTLSRVQFQKLQQLRLTQYHGGSLPNVSQLRSSAPEFQPSFHQADSLRGTRHHGLVERPSRTRFHPLHRRSGDKPGRQFDGSTFGGIYSSQPLDESWPRQQPPWKEEKHPGFRLTSALNRTNSDSALHTSALSTKPQDPYGGGAQSAWPAPYMGFCDGENDAHGEVVSFPGPLKEENLLNVPKPLPKQLWETKEMQSLSGRPRSCDVGGGNTFPHNGQNIGLSPFLGTLNTGGSLPDLTNLHYSTPLPASLDTGDHVFGSMSVGHSVGNLPAAMTHLGLRSSSGLQSSRSNPSIQATLNKTALSSSLSRHPQTSAPSASALHPSLRLFSLSNPSLPTTNLSGPSRRRQPPVSPLTLSPGPEAPQGFSRQLSSTSPLNPYPASQMVSSDRSPLSFLPTEAQAHVSPPPPYPTPQELPQPLLQQPRTQEPPAQQPQAASSLPQSDFPLLTPQGSSLTNFFPDVSFDQQSLRPDPAFTQQVPLVPQGPREPQDSFHLRPNLYSNCGNFPNTVLTEDSSTSLFKDLSSALAGMPEVSLSVDTPFPLEEELQIEPLSLDGLNMLSDSSMGLLDPSVEETFRADRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LIZ0</accession>
    <name>A0A8C4LIZ0_EQUAS</name>
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        <recommendedName>
            <fullName evidence="6">FERM, ARHGEF and pleckstrin domain-containing protein 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">FERM, RhoGEF and pleckstrin domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">FARP2</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="12" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="4">Functions as a guanine nucleotide exchange factor that activates RAC1. May have relatively low activity. Plays a role in the response to class 3 semaphorins and remodeling of the actin cytoskeleton. Plays a role in TNFSF11-mediated osteoclast differentiation, especially in podosome rearrangement and reorganization of the actin cytoskeleton. Regulates the activation of ITGB3, integrin signaling and cell adhesion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Interacts with PLXNA1. Interaction with PLXNA1 or PIP5K1C lowers its guanine nucleotide exchange activity. Dissociates from PLXNA1 when SEMA3A binds to the receptor. Interacts with PIP5K1C via its FERM domain. The interaction with PIP5K1C is enhanced by SEMA3A binding. Interacts with RAC1.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LIZ0"/>
    <dbReference type="Ensembl" id="ENSEAST00005012808.2">
        <property type="protein sequence ID" value="ENSEASP00005011788.2"/>
        <property type="gene ID" value="ENSEASG00005008264.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00940000158642"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 19"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008092">
        <property type="term" value="F:cytoskeletal protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007155">
        <property type="term" value="P:cell adhesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022405">
        <property type="term" value="P:hair cycle process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016322">
        <property type="term" value="P:neuron remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030316">
        <property type="term" value="P:osteoclast differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071800">
        <property type="term" value="P:podosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016601">
        <property type="term" value="P:Rac protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033623">
        <property type="term" value="P:regulation of integrin activation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071526">
        <property type="term" value="P:semaphorin-plexin signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd14473">
        <property type="entry name" value="FERM_B-lobe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13193">
        <property type="entry name" value="FERM_C_FARP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17190">
        <property type="entry name" value="FERM_F1_FARP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01220">
        <property type="entry name" value="PH1_FARP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13235">
        <property type="entry name" value="PH2_FARP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00160">
        <property type="entry name" value="RhoGEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000002">
        <property type="entry name" value="Band 4.1-like protein 5 isoform 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000040">
        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.80.10:FF:000005">
        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000046">
        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.900.10:FF:000020">
        <property type="entry name" value="FERM, RhoGEF and pleckstrin domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.80.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.900.10">
        <property type="entry name" value="Dbl homology (DH) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019749">
        <property type="entry name" value="Band_41_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035899">
        <property type="entry name" value="DBL_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000798">
        <property type="entry name" value="Ez/rad/moesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014847">
        <property type="entry name" value="FA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041788">
        <property type="entry name" value="FARP1/FARP2/FRMD7_FERM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014352">
        <property type="entry name" value="FERM/acyl-CoA-bd_prot_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035963">
        <property type="entry name" value="FERM_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019748">
        <property type="entry name" value="FERM_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019747">
        <property type="entry name" value="FERM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000299">
        <property type="entry name" value="FERM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018979">
        <property type="entry name" value="FERM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018980">
        <property type="entry name" value="FERM_PH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051835">
        <property type="entry name" value="RAC1-GEF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45858">
        <property type="entry name" value="FERM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45858:SF4">
        <property type="entry name" value="FERM, ARHGEF AND PLECKSTRIN DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08736">
        <property type="entry name" value="FA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09380">
        <property type="entry name" value="FERM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="9">Rho GTPase activating protein 9</fullName>
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        <name type="scientific">Equus asinus</name>
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        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
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            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
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            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
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                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
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        <property type="entry name" value="RhoGAP_dom"/>
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            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005010125.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="781" mass="86324" checksum="18FC10D679E8D6F4" modified="2023-09-13" version="2">MRRGKPKGAYTAQKSHHHPGPAPPAPGSSDGPQKVTILNTMLSGRWWPSTWGSLGLGPRSPSRGSQLCALYAFTYTGADGRQVSLVEGDRFLLLRKTNSDWWLARRLGAPSTSRPIFVPAAYVTEESISSQSPTTITPSQTLWTPGPKLFPGSLEKLHLSRKAPGRAQATSEQPPPLPPKMCRSVSVTNLRPTLRQPFQEGPSGRSLSQEDLLMESSADMARPQPLMSEPPVYCNLVDLRRCPRSPPPVPECPPLQRLDAWEQHLDPSSGRCFYINSLTGCKSWKPPRRTRTQETNPGSMDGTQALHRDNGVLQPQAKGSGSDTGTPELPDPQGSLCLRRRTSQLDPSDQPSALQPPRPLPQVLEDPHEVEKSGLLNMTKIAQGGRKLRKNWGPSWVVLAGNSLMFYREPPPPAPSPLWAPTGSRPESSVDLRGAALAHGRHLSSRRNVLHIRTVPGHEFLLQSDQETELRAWHRALRAVIERLDRENPLELRLSGSGPAELGELSAGEDEEEESEPASKPLLRLGGRRSSGRGPEGAEQNRVRNKLKRLIAKRPPLQSLQERGLLRDQVFGCQLESLCQREGDTVPSFVRLCIAAVDKRGLDVDGIYRVSGNLAVVQKLRFLVDRERAITSDGRYVFPEQPGQEGRLDLDSAEWDDIHVVTGALKLFLRELPQPLVPPLLLPKFRAALALSESEQCLSQIRELIGSMPKPNHDTLRYLLEHLCRVIAHSDKNLMTPHNLGIVFGPTLFRPEQETSDPAAHALYPGQLVQLMLTDFASLFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LL13</accession>
    <name>A0A8C4LL13_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Keratin 75</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">KRT75</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="9" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="9" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the intermediate filament family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LL13"/>
    <dbReference type="Ensembl" id="ENSEAST00005014113.2">
        <property type="protein sequence ID" value="ENSEASP00005012984.2"/>
        <property type="gene ID" value="ENSEASG00005009068.2"/>
    </dbReference>
    <dbReference type="KEGG" id="eai:106835252"/>
    <dbReference type="GeneTree" id="ENSGT00940000155862"/>
    <dbReference type="OrthoDB" id="2441647at2759"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001533">
        <property type="term" value="C:cornified envelope"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045095">
        <property type="term" value="C:keratin filament"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030280">
        <property type="term" value="F:structural constituent of skin epidermis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002244">
        <property type="term" value="P:hematopoietic progenitor cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045109">
        <property type="term" value="P:intermediate filament organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031424">
        <property type="term" value="P:keratinization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1160:FF:000001">
        <property type="entry name" value="Keratin type II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.170:FF:000004">
        <property type="entry name" value="Keratin, type II cytoskeletal 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.500:FF:000001">
        <property type="entry name" value="Type II keratin 23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.500">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1160">
        <property type="entry name" value="Vasodilator-stimulated phosphoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018039">
        <property type="entry name" value="IF_conserved"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039008">
        <property type="entry name" value="IF_rod_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032444">
        <property type="entry name" value="Keratin_2_head"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003054">
        <property type="entry name" value="Keratin_II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616">
        <property type="entry name" value="GATA-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45616:SF13">
        <property type="entry name" value="KERATIN, TYPE II CYTOSKELETAL 75"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00038">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16208">
        <property type="entry name" value="Keratin_2_head"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01276">
        <property type="entry name" value="TYPE2KERATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01391">
        <property type="entry name" value="Filament"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64593">
        <property type="entry name" value="Intermediate filament protein, coiled coil region"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00226">
        <property type="entry name" value="IF_ROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51842">
        <property type="entry name" value="IF_ROD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0403">Intermediate filament</keyword>
    <keyword evidence="1" id="KW-0416">Keratin</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="IF rod" evidence="8">
        <location>
            <begin position="147"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="516"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="144"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="202"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="358"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="532"/>
            <end position="549"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022744"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00061646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005012984.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="549" mass="59269" checksum="E333FFB2B9C06882" modified="2023-09-13" version="2">MSRQSTITFQTSGRRGFSTASATTLATGRSRFSSVSTTRSTGGSGGLGRISGAGTSFGSRSLYNLGGTKRVSMSGCGSNFRSGFGGRASGGFGVSGGFGYGGGVGGGYGGSGFPSSGGIQEVTVNQSLLTPLNLQIDPTIQRVRKEEREQIKTLNNKFASFIDKVRFLEQQNKVLETKWSLLQEQGSKTVRQNLEPLFDTYINDLRRQLDSITSERGRLDAELRNMQDVVEDFKVRYEDEINKRTAAENEFVALKKDVDAAYMNKVELEAKVNCLTDEINCFQTLFETELSQMQTRISDTSVVLSMDNNRSLDLDSIIAEVKAQYEDIANRSRAEAESWYQTKYEELQVTAGRHGDDLRNTKQEISEMNRMIQRLRAEVDSVKKQCSSLQTAIADGEQRGELALKDARAKLVELEEALQKAKQDMARLLREYQELMNVKLALDVEIATYRKLLEGEECRLSGEGVSPVNISVVTSTVSSGYGGSSGIGGGSLGLSGGSGYSFTTSGGHGLGAGLGGSGFSASSSRGPGGSGSSVKFVSTTSSSRKSYKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LLQ0</accession>
    <name>A0A8C4LLQ0_EQUAS</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Piwi like RNA-mediated silencing 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">PIWIL3</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="14" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="14" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the argonaute family. Piwi subfamily.</text>
    </comment>
    <dbReference type="RefSeq" id="XP_044631613.1">
        <property type="nucleotide sequence ID" value="XM_044775678.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="A0A8C4LLQ0"/>
    <dbReference type="Ensembl" id="ENSEAST00005012251.2">
        <property type="protein sequence ID" value="ENSEASP00005011266.2"/>
        <property type="gene ID" value="ENSEASG00005007918.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106841691"/>
    <dbReference type="KEGG" id="eai:106841691"/>
    <dbReference type="CTD" id="440822"/>
    <dbReference type="GeneTree" id="ENSGT00950000183200"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 8"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031047">
        <property type="term" value="P:regulatory ncRNA-mediated gene silencing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007283">
        <property type="term" value="P:spermatogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02845">
        <property type="entry name" value="PAZ_piwi_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04658">
        <property type="entry name" value="Piwi_piwi-like_Euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000014">
        <property type="entry name" value="Piwi-like RNA-mediated gene silencing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.260.10:FF:000003">
        <property type="entry name" value="Piwi-like RNA-mediated gene silencing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.260.10">
        <property type="entry name" value="paz domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031320">
        <property type="entry name" value="GAGE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003100">
        <property type="entry name" value="PAZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036085">
        <property type="entry name" value="PAZ_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05831">
        <property type="entry name" value="GAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02170">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23278">
        <property type="entry name" value="Piwi_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01379">
        <property type="entry name" value="GAGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00949">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101690">
        <property type="entry name" value="PAZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50821">
        <property type="entry name" value="PAZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0217">Developmental protein</keyword>
    <keyword evidence="4" id="KW-0221">Differentiation</keyword>
    <keyword evidence="9" id="KW-0469">Meiosis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8" id="KW-0943">RNA-mediated gene silencing</keyword>
    <keyword evidence="6" id="KW-0744">Spermatogenesis</keyword>
    <keyword evidence="5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="PAZ" evidence="12">
        <location>
            <begin position="297"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="domain" description="Piwi" evidence="13">
        <location>
            <begin position="574"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="16"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011266.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="880" mass="100322" checksum="6D6B53B3A1776DCC" modified="2023-09-13" version="2">MSGRARTRARGRARGRASPQPAAPGASAAPGAAAPGASAAPGGATTTRQPGQILPRPQPLPAESQVVEPRQPRGAEGTSQPSAELQLSAGLQAASLRENRGFSGVFRDLVVNTRQNLEHVRKSKTGSKGTVVRLFANHFRVTSRPQWVTYQYNVDYKPDIEDGSLRADLLKQHESLLGKCRIFDGNSLLLPRVLRKRKMEVVSKYKEQPVKITIEFSRELTPTSPDCLRYYNILFRKILKSMELKQIGRNYYNKCGATQFLDHSLEVWPGYFTSILEYENSITLCADVSHKLLRMETAYDVIMRIVDTPREGDVKQRVSKALVGLIVLTKHNNRTYRVDAINWEETPKATFKKSDGNRITFVDYYRQQYDTQVTDLTQPLLISKGKWKKSQQDTPNEPVLLVPELCSLTGLTDAMRQDYRVMRDLALHMRLDPDKRQHELQKFMNTVQRDKTAQEELRLWDLKFDTNFLSFSGRLLKEVRICQGRRVFNSHPLFAEWSKETRKGPLLKVKSLDDWLMLYTKRNYGAAISLMQCLRKVTPTMGIAMRDAKMLEVNDTVKSYINVLEKRASSKTQMVVCVLSSDRKEMYDGIKKFLCVNYPIPSQCVVARTLDKPQTLMTIATKIAQQMNCKMGGALWKVETGLRNTMFIGIDCFHDIVNRRKSIAGFVSSVNDELTQWFSQCIFQESGQELVNGLRDCLQAALKLWREHNPFMPRSVIVYRDGVGDGQLEALIKYEIPQISSCLESILQTNDVKLAVIVVKKRINTRFFVESEGKLRNPRPGTVVDVELTRKEWYDFFIVSQSVKDGTVTPTHYNVIHDTVRLSPDTVQGLTYRLCHMYYNLPGVIRVPAPCHYAHKLAYLVGQSIHQEPHHSLTNRLFYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LMI1</accession>
    <name>A0A8C4LMI1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">5'-deoxynucleotidase HDDC2</fullName>
            <ecNumber evidence="8">3.1.3.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">HD domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">HDDC2</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="15" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="15" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the dephosphorylation of the nucleoside 5'-monophosphates deoxyadenosine monophosphate (dAMP), deoxycytidine monophosphate (dCMP), deoxyguanosine monophosphate (dGMP) and deoxythymidine monophosphate (dTMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'-deoxyribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:36167"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18274"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:65317"/>
            <dbReference type="EC" id="3.1.3.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the HDDC2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.89" evidence="8"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4LMI1"/>
    <dbReference type="Ensembl" id="ENSEAST00005014588.2">
        <property type="protein sequence ID" value="ENSEASP00005013429.2"/>
        <property type="gene ID" value="ENSEASG00005009371.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000009937"/>
    <dbReference type="OMA" id="TWRLCLM"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 24"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002953">
        <property type="term" value="F:5'-deoxynucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009159">
        <property type="term" value="P:deoxyribonucleoside monophosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3210.10:FF:000011">
        <property type="entry name" value="HD domain-containing protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039356">
        <property type="entry name" value="YfbR/HDDC2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11845:SF13">
        <property type="entry name" value="5'-DEOXYNUCLEOTIDASE HDDC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13023">
        <property type="entry name" value="HD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="14">
        <location>
            <begin position="45"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00015933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005013429.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="203" mass="23396" checksum="0DED410A10BD0019" modified="2023-09-13" version="2">MASASVPLAGRGVRDLLRFLRLVGQLKRVPRTGWVYRNVERPESVSDHMYRMAVMALVTKDEHLNKDRCVRLALVHDMAECIVGDIAPADNIPKEEKHRREEEAMKQLTRLLPEDLRKELYELWEEYETQSSAEARFVKQLDQCEMILQASEYEDLENKPGRLQDFYDSTAGKFSHPEIVQLVSELEAERNANIAATTSEPHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LPD1</accession>
    <name>A0A8C4LPD1_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Zinc finger protein 219</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="primary">ZNF219</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="20" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="20" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">Transcriptional regulator. Recognizes and binds 2 copies of the core DNA sequence motif 5'-GGGGG-3'. Binds to the HMGN1 promoter and may repress HMGN1 expression. Regulates SNCA expression in primary cortical neurons. Binds to the COL2A1 promoter and activates COL2A1 expression, as part of a complex with SOX9. Plays a role in chondrocyte differentiation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Interacts with SOX9 (via C-terminus).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="AlphaFoldDB" id="A0A8C4LPD1"/>
    <dbReference type="Ensembl" id="ENSEAST00005016191.2">
        <property type="protein sequence ID" value="ENSEASP00005014883.1"/>
        <property type="gene ID" value="ENSEASG00005010408.2"/>
    </dbReference>
    <dbReference type="GeneID" id="106833268"/>
    <dbReference type="KEGG" id="eai:106833268"/>
    <dbReference type="CTD" id="51222"/>
    <dbReference type="GeneTree" id="ENSGT00940000161408"/>
    <dbReference type="OMA" id="EESWTRG"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 2"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001227">
        <property type="term" value="F:DNA-binding transcription repressor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060174">
        <property type="term" value="P:limb bud formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032332">
        <property type="term" value="P:positive regulation of chondrocyte differentiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000075">
        <property type="entry name" value="Putative zinc finger protein 536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001038">
        <property type="entry name" value="Zinc finger protein 217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:001140">
        <property type="entry name" value="Zinc finger protein 219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000591">
        <property type="entry name" value="Zinc finger protein 536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000615">
        <property type="entry name" value="Zinc finger protein 536"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051967">
        <property type="entry name" value="Krueppel_C2H2-ZF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45925">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45925:SF1">
        <property type="entry name" value="ZINC FINGER PROTEIN 219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0010">Activator</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0678">Repressor</keyword>
    <keyword evidence="12" id="KW-0804">Transcription</keyword>
    <keyword evidence="9" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 17" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="57"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="85"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="268"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="296"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="491"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="19">
        <location>
            <begin position="519"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="135"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="215"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="383"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="535"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="661"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="227"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="253"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="419"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="18">
        <location>
            <begin position="551"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="583"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="18">
        <location>
            <begin position="627"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="661"/>
            <end position="670"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00064680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00067251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005014883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="715" mass="76087" checksum="7C25899BCB66070F" modified="2022-01-19" version="1">MEGSRPRAPGGHLAPSPPAFDGELDLQRYSNGPGVSAGSPGMGAVGWSESRTGERRFPCPVCGKRFRFNSILALHLRAHPGAQAFQCPHCGHRAAQRALLRSHLRTHQPERPRSPAARLLLELEERALLREARLGRARSSGGVHATSATESLARPQAPSSSAFRCPFCKGKFRTAAERERHLHILHRPWKCGLCSFGSSQEEELLHHSLTAHGAPERPLAATSAAPQPQPPPQPEPRSVPEPDAEPEREATPAPAPAAPEEPPAPPEFRCQVCGQSFTQSWFLKGHMRKHKASFDHACPVCGRCFKEPWFLKNHMKVHASKLGPLRAPGPGSGPARAPQPPDLGLLAYEPLGPALLLAPAPTPAERREPPSLLGYLSLRAGEARPNGEGAEPGAGRSFGGFRPLPSALPARARRHRAEEPDEEEEVVEAEEESWARSRALGPLASLHPRPGEGPHSAPAAGAQARSTSTQEENGLLVGGTRPEGNRGATGKDCPFCGKSFRSAHHLKVHLRVHTGERPYKCPHCDYAGTQSGSLKYHLQRHHREQRSGAGPGPPPEPPPPSQRGSAQQSGAKPAPQPATWVEGAASPRPPSSGAAPGSRRKPASPGRTLRNGRGGEAEPLDLSLRAGPGGEAGPGGALHRCLFCPFATGAPELMALHLQVHHSRRARGRRPPQANASPPYARAPSGETPPSPSQEGEESPGLSRSGEAGLGGQER</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LQ67</accession>
    <name>A0A8C4LQ67_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">DNA helicase B</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="primary">HELB</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Equus asinus</name>
        <name type="common">Donkey</name>
        <name type="synonym">Equus africanus asinus</name>
        <dbReference type="NCBI Taxonomy" id="9793"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Metazoa</taxon>
            <taxon>Chordata</taxon>
            <taxon>Craniata</taxon>
            <taxon>Vertebrata</taxon>
            <taxon>Euteleostomi</taxon>
            <taxon>Mammalia</taxon>
            <taxon>Eutheria</taxon>
            <taxon>Laurasiatheria</taxon>
            <taxon>Perissodactyla</taxon>
            <taxon>Equidae</taxon>
            <taxon>Equus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2020" name="Nat. Commun." volume="11" first="6014" last="6014">
            <title>Donkey genomes provide new insights into domestication and selection for coat color.</title>
            <authorList>
                <person name="Wang"/>
                <person name="C."/>
                <person name="Li"/>
                <person name="H."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Huang"/>
                <person name="J."/>
                <person name="Sun"/>
                <person name="Y."/>
                <person name="Min"/>
                <person name="J."/>
                <person name="Wang"/>
                <person name="J."/>
                <person name="Fang"/>
                <person name="X."/>
                <person name="Zhao"/>
                <person name="Z."/>
                <person name="Wang"/>
                <person name="S."/>
                <person name="Zhang"/>
                <person name="Y."/>
                <person name="Liu"/>
                <person name="Q."/>
                <person name="Jiang"/>
                <person name="Q."/>
                <person name="Wang"/>
                <person name="X."/>
                <person name="Guo"/>
                <person name="Y."/>
                <person name="Yang"/>
                <person name="C."/>
                <person name="Wang"/>
                <person name="Y."/>
                <person name="Tian"/>
                <person name="F."/>
                <person name="Zhuang"/>
                <person name="G."/>
                <person name="Fan"/>
                <person name="Y."/>
                <person name="Gao"/>
                <person name="Q."/>
                <person name="Li"/>
                <person name="Y."/>
                <person name="Ju"/>
                <person name="Z."/>
                <person name="Li"/>
                <person name="J."/>
                <person name="Li"/>
                <person name="R."/>
                <person name="Hou"/>
                <person name="M."/>
                <person name="Yang"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="W."/>
                <person name="Guo"/>
                <person name="J."/>
                <person name="Pan"/>
                <person name="S."/>
                <person name="Fan"/>
                <person name="G."/>
                <person name="Zhang"/>
                <person name="W."/>
                <person name="Zhang"/>
                <person name="R."/>
                <person name="Yu"/>
                <person name="J."/>
                <person name="Zhang"/>
                <person name="X."/>
                <person name="Yin"/>
                <person name="Q."/>
                <person name="Ji"/>
                <person name="C."/>
                <person name="Jin"/>
                <person name="Y."/>
                <person name="Yue"/>
                <person name="G."/>
                <person name="Liu"/>
                <person name="M."/>
                <person name="Xu"/>
                <person name="J."/>
                <person name="Liu"/>
                <person name="S."/>
                <person name="Jordana"/>
                <person name="J."/>
                <person name="Noce"/>
                <person name="A."/>
                <person name="Amills"/>
                <person name="M."/>
                <person name="Wu"/>
                <person name="D.D."/>
                <person name="Li"/>
                <person name="S."/>
                <person name="Zhou"/>
                <person name="X. and Zhong"/>
                <person name="J."/>
            </authorList>
            <dbReference type="PubMed" id="33293529"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
    </reference>
    <reference evidence="19" key="2">
        <citation type="submission" date="2025-08" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <reference evidence="19" key="3">
        <citation type="submission" date="2025-09" db="UniProtKB">
            <authorList>
                <consortium name="Ensembl"/>
            </authorList>
        </citation>
        <scope>IDENTIFICATION</scope>
    </reference>
    <comment type="function">
        <text evidence="14">5'-3' DNA helicase involved in DNA damage response by acting as an inhibitor of DNA end resection. Recruitment to single-stranded DNA (ssDNA) following DNA damage leads to inhibit the nucleases catalyzing resection, such as EXO1, BLM and DNA2, possibly via the 5'-3' ssDNA translocase activity of HELB. As cells approach S phase, DNA end resection is promoted by the nuclear export of HELB following phosphorylation. Acts independently of TP53BP1. Unwinds duplex DNA with 5'-3' polarity. Has single-strand DNA-dependent ATPase and DNA helicase activities. Prefers ATP and dATP as substrates. During S phase, may facilitate cellular recovery from replication stress.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the RecD family. HELB subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="AlphaFoldDB" id="A0A8C4LQ67"/>
    <dbReference type="Ensembl" id="ENSEAST00005012316.2">
        <property type="protein sequence ID" value="ENSEASP00005011324.2"/>
        <property type="gene ID" value="ENSEASG00005007965.2"/>
    </dbReference>
    <dbReference type="GeneTree" id="ENSGT00390000006913"/>
    <dbReference type="Proteomes" id="UP000694387">
        <property type="component" value="Chromosome 22"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016604">
        <property type="term" value="C:nuclear body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000042">
        <property type="term" value="P:negative regulation of double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903775">
        <property type="term" value="P:regulation of DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="Ensembl"/>
    </dbReference>
    <dbReference type="CDD" id="cd17933">
        <property type="entry name" value="DEXSc_RecD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18809">
        <property type="entry name" value="SF1_C_RecD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001523">
        <property type="entry name" value="Helicase (DNA) B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058839">
        <property type="entry name" value="WHD_HELB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF6">
        <property type="entry name" value="DNA HELICASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13604">
        <property type="entry name" value="AAA_30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25894">
        <property type="entry name" value="WHD_HELB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase B winged helix" evidence="18">
        <location>
            <begin position="243"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="420"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="951"/>
            <end position="1054"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="173"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="424"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="957"/>
            <end position="977"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00061441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00072281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF25894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Ensembl" id="ENSEASP00005011324.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000694387"/>
        </source>
    </evidence>
    <sequence length="1078" mass="121927" checksum="E7204727094B629A" modified="2023-09-13" version="2">TAMAGVQWTVREVRGRLLPFKDLAEEDDDYLKEDAEEDDDPGFVDAEELCSGGLRAGGLPGRQRVMILDENTQEQCKVFGRFPMMDPWWRVKLQVKPVGSRKCYHVQGFPSYFLLSDMSPPDQKQICSLFLKECAIPDDIKMKFLDWVDEVSSFNDLNFENLNETFRTFLKNGRNDKKQSTQNEREESLPGNETSLSLENKIPFINVMRALQFPKVMEFLPVLLPRHFKQLISSGSQEVLGKIEEILRTHPWKLGFHKITFRELKLLRCEASWAAFCQCKSLFRLMNSFEKNGLIMYSELKRQCKEHGHTYVEEADLTSLLSDRMSVDDAWQSLRFLKDVGVVTYEKACVFLYDLYEAERGIASAICHLMTRPPWHLHVDVRELLGSMQTKGAGSSGGDDTLKESKADETGLENSLAILDTQDSGDHTGDNGEHEVHAEESEVQLDLDQVAALEMICSNAVTVMSGKGGCGKTTIVSHLFKHVELLEESEAERVCEDFKRDQDVSEEWLNSPGQRQPEADKAIKVLLTAPTGKAAGLLRQRTDFSAYTLYQVNYSFYSWKTKTAQDTPWKFSSVRVLVVDEGSLVSVGIFKSVLKLLCEHARLSKLIILGDVRQLPSIEPGNLLQDLFETLKPRKCAIELRTNHRAESELIVDNATRISRRQFPRFDAELNLSDNATFPVSIQDKTFVFVRLPEEDDSSQLSKDDHRAHLYSAVRTLLKEDDLRDAEKSQFIAFRRQDCDVINDCCCRHYTDHLMKDHQKRLQFGVGDKICCTRNAYLSDLLPADTLNSQQNNDFAPSGADFNGTPAGFANKHDFDSGIRLCNGEIFFITKDVTDVTFGRRRLVTIDNRAGLEVTVDFQKLMKHCHVKHAWARTIHTFQGSEERTVVYVVGKAGRQHWQHVYTAVTRGRCRVYVIAEESQLRSAISRNSFRRRTRLKHFLQNNLSMSYASPADFASPSKSSGDSGGHSTQPSASPGPTVTADLGTNDVPRSKASAADGGAFSSPRRWKFSSPEDVDMDEDLSTSRGCKRTCAMDNTESPNKVLMVDETSPQVSSRLQTLKLNSLTPRRLFKSTDNQET</sequence>
</entry>
<entry dataset="TrEMBL" created="2022-01-19" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>A0A8C4LSM6</accession>
    <name>A0A8C4LSM6_EQUAS</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Cytochrome c1, heme protein, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Complex III subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Complex III subunit IV</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15">Cytochrome b-c1 complex subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="19">Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit</
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